Ins1 cDNA ORF clone, Mus musculus(house mouse)
| Gene Symbol | Ins1 |
| Entrez Gene ID | 16333 |
| Full Name | insulin I |
| Synonyms | Ins-1,Ins2-rs1 |
| General protein information |
|
| Gene Type | protein-coding |
| Organism | Mus musculus(house mouse) |
| Genome | |
| Summary | This gene encodes insulin, a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. The encoded precursor protein undergoes proteolytic cleavage to produce a disulfide-linked heterodimeric functional protein that is stored in secretory granules. An increase in blood glucose levels, among others, induces the release of insulin from the secretory granules. Mice deficient in the functional hormone encoded by this gene develop diabetes mellitus. [provided by RefSeq, Aug 2015]. |
mRNA and Protein(s)
| mRNA | Protein | Name |
|---|---|---|
| NM_008386.4 | NP_032412.3 | insulin-1 preproprotein |
Related articles in PubMed
Amino acid sequence of the two insulins from mouse (Maus musculus).
B?nzli HF, Glatthaar B, Kunz P, M?lhaupt E, Humbel RE
Hoppe-Seyler's Zeitschrift fur physiologische Chemie353(3)451-8(1972 Mar)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
Despite higher endogenous insulin concentrations following feeding, arcuate nucleus phosphorylation of Akt (pAkt) levels were significantly lower in the pregnant group compared with the nonpregnant group.
Title: Region-Specific Suppression of Hypothalamic Responses to Insulin To Adapt to Elevated Maternal Insulin Secretion During Pregnancy.
insulin and aPC converge on a common spliced-X-box binding protein-1 (sXBP1) signaling pathway to maintain endoplasmic reticulum (ER) homeostasis.
Title: Signal integration at the PI3K-p85-XBP1 hub endows coagulation protease activated protein C with insulin-like function.
Insulin stimulation of Akt1 and Akt2 signaling in Cystic fibrosis airway cells was diminished compared with that observed in airway cells expressing wild-type CFTR.
Title: Insulin signaling via the PI3-kinase/Akt pathway regulates airway glucose uptake and barrier function in a CFTR-dependent manner.
These data implicate the insulin-FoxM1/PLK1/CENP-A pathway-regulated mitotic cell-cycle progression as an essential component in the beta cell adaptation to delay and/or prevent progression to diabetes.
Title: Insulin Signaling Regulates the FoxM1/PLK1/CENP-A Pathway to Promote Adaptive Pancreatic ??Cell Proliferation.
BMP-7 therefore is an attractive candidate for tackling a multifaceted disease such as diabetes, since it not only reduces body fat, but also strengthens insulin signaling, causing improved glucose uptake and ameliorating peripheral insulin resistance.
Title: Bone morphogenetic protein-7 (BMP-7) augments insulin sensitivity in mice with type II diabetes mellitus by potentiating PI3K/AKT pathway.
The following Ins1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ins1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OMu19250 | |
| Clone ID Related Accession (Same CDS sequence) | NM_008386.4 | |
| Accession Version | NM_008386.4 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 327bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-16 | |
| Organism | Mus musculus(house mouse) | |
| Product | insulin-1 preproprotein | |
| Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC163452.12, BP773571.1, AK008358.1 and BQ126282.1. On Aug 11, 2015 this sequence version replaced NM_008386.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BP773571.1, BP776899.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849377 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGCCCTGT TGGTGCACTT CCTACCCCTG CTGGCCCTGC TTGCCCTCTG GGAGCCCAAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_032412.3 |
| CDS | 210..536 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_008386.4 |
ORF Insert Sequence:
1 | ATGGCCCTGT TGGTGCACTT CCTACCCCTG CTGGCCCTGC TTGCCCTCTG GGAGCCCAAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
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Amino acid sequence of the two insulins from mouse (Maus musculus). |