This gene encodes a member of the small leucine-rich proteoglycan family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protein. This protein plays a role in collagen fibril assembly. Binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis. This gene and the related gene biglycan are thought to be the result of a gene duplication. Mutations in this gene are associated with congenital stromal corneal dystrophy in human patients. [provided by RefSeq, Nov 2015].
The following DCN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DCN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA663155.1, CD689331.1, BX571756.1, BM672011.1, AC007115.1, BX094949.1 and AW444736.1. This sequence is a reference standard in the RefSeqGene project. On Nov 7, 2015 this sequence version replaced NM_001920.3.
Transcript Variant: This variant (A1) represents the longest transcript and encodes the longest isoform. Both variants A1 and A2 encode the same isoform (a).
Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.
##Evidence-Data-START##
Transcript exon combination :: SRR1803617.83344.1, SRR1660807.110475.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348]
##Evidence-Data-END##
COMPLETENESS: complete on the 3' end.
Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA424310.1, BX571756.1, BM672011.1, AC007115.1, BX094949.1 and AW444736.1. On Nov 7, 2015 this sequence version replaced NM_133503.2.
Transcript Variant: This variant (A2) differs in the 5' UTR compared to variant A1. Both variants A1 and A2 encode the same isoform (a).
Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.
##Evidence-Data-START##
Transcript exon combination :: SRR1660807.218235.1, SRR1660805.207126.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348]
##Evidence-Data-END##
COMPLETENESS: complete on the 3' end.
Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process
##Genome-Annotation-Data-START##
Annotation Provider :: NCBI
Annotation Status :: Full annotation
Annotation Version :: Homo sapiens Annotation Release 108
Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline
Annotation Software Version :: 7.0
Annotation Method :: Best-placed RefSeq; Gnomon
Features Annotated :: Gene; mRNA; CDS; ncRNA
##Genome-Annotation-Data-END##
Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process
##Genome-Annotation-Data-START##
Annotation Provider :: NCBI
Annotation Status :: Full annotation
Annotation Version :: Homo sapiens Annotation Release 108
Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline
Annotation Software Version :: 7.0
Annotation Method :: Best-placed RefSeq; Gnomon
Features Annotated :: Gene; mRNA; CDS; ncRNA
##Genome-Annotation-Data-END##
Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process
##Genome-Annotation-Data-START##
Annotation Provider :: NCBI
Annotation Status :: Full annotation
Annotation Version :: Homo sapiens Annotation Release 108
Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline
Annotation Software Version :: 7.0
Annotation Method :: Best-placed RefSeq; Gnomon
Features Annotated :: Gene; mRNA; CDS; ncRNA
##Genome-Annotation-Data-END##
Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL517167.3, DA424244.1, AF138303.1, BX571756.1, BM672011.1, AC007115.1, BX094949.1 and AW444736.1. On Nov 7, 2015 this sequence version replaced NM_133506.2.
Transcript Variant: This variant (D) differs in the 5' UTR and lacks four alternate exons in the coding region compared to variant A1. The encoded isoform (d) is shorter than isoform a. This isoform (d) may undergo proteolytic processing similar to isoform a.
Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.
##Evidence-Data-START##
Transcript exon combination :: AF138303.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns SAMEA2145245, SAMEA2151358 [ECO:0000348]
##Evidence-Data-END##
COMPLETENESS: complete on the 3' end.
Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL517167.3, DA424244.1, AF138304.1, AC007115.1, BX571756.1, BM672011.1, BX094949.1 and AW444736.1. On Nov 7, 2015 this sequence version replaced NM_133507.2.
Transcript Variant: This variant (E) differs in the 5' UTR and lacks five alternate exons in the coding region compared to variant A1. The encoded isoform (e) is shorter than isoform a. This isoform (e) may undergo proteolytic processing similar to isoform a.
Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.
##Evidence-Data-START##
RNAseq introns :: single sample supports all introns SAMEA2145245, SAMEA2158569 [ECO:0000348]
##Evidence-Data-END##
COMPLETENESS: complete on the 3' end.
Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL517167.3, DA424244.1, AF138302.1, BX571756.1, BM672011.1, AC007115.1, BX094949.1 and AW444736.1. On Nov 7, 2015 this sequence version replaced NM_133505.2.
Transcript Variant: This variant (C) differs in the 5' UTR and lacks three alternate exons in the coding region compared to variant A1. The encoded isoform (c) is shorter than isoform a. This isoform (c) may undergo proteolytic processing similar to isoform a.
Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.
##Evidence-Data-START##
Transcript exon combination :: AF138302.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348]
##Evidence-Data-END##
COMPLETENESS: complete on the 3' end.
Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL517167.3, DA424244.1, AF138301.1, BX571756.1, BM672011.1, AC007115.1, BX094949.1 and AW444736.1. On Nov 7, 2015 this sequence version replaced NM_133504.2.
Transcript Variant: This variant (B) differs in the 5' UTR and lacks two alternate exons in the coding region compared to variant A1. The encoded isoform (b) is shorter than isoform a. This isoform (b) may undergo proteolytic processing similar to isoform a.
Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.
##Evidence-Data-START##
Transcript exon combination :: AF138301.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns SAMEA2467150 [ECO:0000348]
##Evidence-Data-END##
COMPLETENESS: complete on the 3' end.
User Manual
NM_133503.2
