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uaf-1 cDNA ORF clone, Caenorhabditis elegans

Summary

Gene Symbol uaf-1
Entrez Gene ID 175270
Full Name Splicing factor U2AF 65 kDa subunit
Gene Type protein-coding
Organism Caenorhabditis elegans

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_064925.6 NP_497326.2 Splicing factor U2AF 65 kDa subunit
NM_001027799.1 NP_001022970.1 U2 snRNP auxiliary factor large subunit
NM_001027796.4 NP_001022967.1 Splicing factor U2AF 65 kDa subunit
NM_001373843.1 NP_001359811.1 Splicing factor U2AF 65 kDa subunit
NM_001027798.1 NP_001022969.1 Splicing factor U2AF 65 kDa subunit
NM_001027796.3 NP_001022967.1 Splicing factor U2AF 65 kDa subunit

Pathways from BioSystems


Homologies


Homo sapiens (human) U2AF2 NP_009210.1
Mus musculus (house mouse) U2af2 NP_598432.2
Danio rerio (zebrafish) u2af2b NP_991252.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP002908 XP_311994.4
Bos taurus (cattle) U2AF2 NP_001068804.1
Neurospora crassa NCU03039 XP_964044.1
Arabidopsis thaliana (thale cress) ATU2AF65A NP_001190937.1
Oryza sativa (rice) Os11g0682300 NP_001068459.1
Canis lupus familiaris (dog) U2AF2 XP_005616162.1
Arabidopsis thaliana (thale cress) AT1G60900 NP_176287.3
Oryza sativa (rice) Os11g0636900 NP_001068330.1
Xenopus tropicalis (tropical clawed frog) u2af2 NP_001016998.1
Rattus norvegicus (Norway rat) U2af2 XP_006228382.1
Magnaporthe oryzae (rice blast fungus) MGG_00348 XP_003718735.1
Danio rerio (zebrafish) u2af2a NP_001025127.1
Drosophila melanogaster (fruit fly) U2af50 NP_476891.1
Caenorhabditis elegans (roundworm) uaf-1 NP_001022967.1
Schizosaccharomyces pombe (fission yeast) prp2 NP_595396.1

Gene Ontology


Bibliography

Related articles in PubMed

GeneRIFs: Gene References Into Functions What's a GeneRIF?

WDR-20 and WDR-48 form a complex with USP-46 and stimulate the DUB to deubiquitinate and stabilize GLR-1 in vivo.
Title: The WD40-repeat proteins WDR-20 and WDR-48 bind and activate the deubiquitinating enzyme USP-46 to promote the abundance of the glutamate receptor GLR-1 in the ventral nerve cord of Caenorhabditis elegans.

The interaction of TEG-1 CD2BP2 with UAF-1 U2AF65, combined with its previously described function in U4/U6.U5 tri-snRNP, suggests that TEG-1 CD2BP2 functions in two distinct locations in the splicing cascade.
Title: TEG-1 CD2BP2 regulates stem cell proliferation and sex determination in the C. elegans germ line and physically interacts with the UAF-1 U2AF65 splicing factor.

Mutations in the splicing factors U2AF large subunit altered the splicing of tos-1. 3' splice sites.
Title: In vivo effects on intron retention and exon skipping by the U2AF large subunit and SF1/BBP in the nematode Caenorhabditis elegans.

observations provide in vivo evidence that UAF-1 can act in regulating 3' splice-site choice and establish a system that can be used to investigate the in vivo regulation of RNA splicing in C. elegans.
Title: Mutations in the Caenorhabditis elegans U2AF large subunit UAF-1 alter the choice of a 3' splice site in vivo.

The following uaf-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the uaf-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCa340822 NM_001027796.4
Latest version!		 Caenorhabditis elegans Splicing factor U2AF 65 kDa subunit (uaf-1), partial mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $223.30
$319.00
OCa340823 NM_001373843.1
Latest version!		 Caenorhabditis elegans Splicing factor U2AF 65 kDa subunit (uaf-1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $69.30
$99.00
OCa340823 NM_064925.6
Latest version!		 Caenorhabditis elegans Splicing factor U2AF 65 kDa subunit (uaf-1), partial mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $104.30
$149.00
OCa340824 NM_001027798.1
Latest version!		 Caenorhabditis elegans Splicing factor U2AF 65 kDa subunit (uaf-1), partial mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $258.30
$369.00
OCa340825 NM_001027799.1
Latest version!		 Caenorhabditis elegans U2 snRNP auxiliary factor large subunit (uaf-1), partial mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $258.30
$369.00
View Old Accession Versions >>
OCa340822 NM_001027796.3 Caenorhabditis elegans Splicing factor U2AF 65 kDa subunit (uaf-1), partial mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $258.30
$369.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OCa340822
Clone ID Related Accession (Same CDS sequence) NM_001027796.4 , NM_001027796.3
Accession Version NM_001027796.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1491bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-10-17
Organism Caenorhabditis elegans
Product Splicing factor U2AF 65 kDa subunit
Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_001027796.3. COMPLETENESS: incomplete on the 5' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGAGTGATC ACCAGGATGG CATGAAACTT GAAGATATTG AACGCCAATT TTTGGACGTT 
GCTCAACGTG AAGGAGGTCT CGAAGCGATT CAGCCGACCA CTGGACCACT GGAAAATGAA 
GAAAATCTGA AATCATCGAC TGGTGGTGGT GGTGGAGAGG ATGATAATGA TCGGAAAAAG 
AGAAAACGAT CGAGAAGTAG AGATCGCGAT ACGAGAAGAC GCTCGAGAAG TCGTGATCGT 
GGAGAACGTC GTGGTGGTGG AGGTGGAGGT GATCGTGATC GTTCTCGATC CCGTGAACGT 
CGTCGAGGCG GTGGTGGTCG TGATGAGCCG AGACGTCGTG GAGGTGATGA TGAAGCCCGT 
TCGCGCCGTG AGCCGGAGCC CCAGAAGCCT CGTGAGCCCA AAAAGTACCG ATTCTGGGAT 
GTTCCGCCGA CTGGATTCGA GACAACTACA CCGATGGAGT ACAAGAATAT GCAGGCGGCT 
GGACAGGTTC CACGTGGATC TGTACAGTCT GCAGTGCCCG TTGTTGGCCC ATCAGTGACT 
TGTCAATCAC GTCGTCTCTA CGTTGGAAAT ATTCCGTTCG GATGCAATGA AGAAGCTATG 
CTCGACTTTT TCAATCAACA AATGCACCTC TGTGGATTGG CTCAAGCGCC TGGAAATCCG 
ATTCTTCTGT GCCAAATAAA TTTGGACAAA AATTTCGCAT TCATAGAATT CCGTTCAATA 
GACGAAACGA CGGCCGGAAT GGCATTTGAC GGAATCAATT TCATGGGACA ACAGTTGAAA 
GTTCGACGTC CAAGAGACTA TCAACCATCT CAAAATACGT TTGATATGAA CTCTCGTATG 
CCTGTTTCAA CGATTGTCGT CGATTCGGCC AATAAGATCT TTATCGGTGG TCTGCCCAAC 
TATTTGACTG AGGATCAGGT AAAAGAGCTT TTGTGCTCAT TTGGACCGCT TAAGGCGTTC 
TCGTTGAACG TTGATTCGCA AGGAAATAGC AAAGGATACG CGTTCGCCGA ATATTTGGAC 
CCAACTCTCA CTGATCAGGC CATCGCCGGG CTCAACGGTA TGCAACTCGG CGACAAGCAG 
CTCGTCGTTC AACTTGCCTG TGCTAATCAA CAACGTCATA ACACAAATCT GCCGAACTCT 
GCGTCAGCAA TTGCCGGAAT TGATCTCTCC CAAGGAGCCG GCCGAGCCAC TGAGATCCTT 
TGTCTCATGA ATATGGTGAC TGAGGATGAG CTGAAAGCTG ATGATGAGTA CGAAGAGATT 
CTGGAGGATG TACGCGATGA ATGCTCAAAA TATGGAATTG TTCGCTCGTT GGAAATTCCA 
AGACCGTATG AGGATCATCC GGTTCCAGGT GTTGGGAAGG TTTTCGTCGA ATTCGCCTCC 
ACATCCGACT GTCAACGTGC TCAGGCTGCT CTGACCGGAC GAAAATTCGC GAATCGCACC 
GTCGTCACCT CCTACTATGA CGTCGACAAG TACCACAATC GTCAATTCTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001022967.1
CDS1..1491
Translation

Target ORF information:

RefSeq Version NM_001027796.4
Organism Caenorhabditis elegans
Definition Caenorhabditis elegans Splicing factor U2AF 65 kDa subunit (uaf-1), partial mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001027796.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGAGTGATC ACCAGGATGG CATGAAACTT GAAGATATTG AACGCCAATT TTTGGACGTT 
GCTCAACGTG AAGGAGGTCT CGAAGCGATT CAGCCGACCA CTGGACCACT GGAAAATGAA 
GAAAATCTGA AATCATCGAC TGGTGGTGGT GGTGGAGAGG ATGATAATGA TCGGAAAAAG 
AGAAAACGAT CGAGAAGTAG AGATCGCGAT ACGAGAAGAC GCTCGAGAAG TCGTGATCGT 
GGAGAACGTC GTGGTGGTGG AGGTGGAGGT GATCGTGATC GTTCTCGATC CCGTGAACGT 
CGTCGAGGCG GTGGTGGTCG TGATGAGCCG AGACGTCGTG GAGGTGATGA TGAAGCCCGT 
TCGCGCCGTG AGCCGGAGCC CCAGAAGCCT CGTGAGCCCA AAAAGTACCG ATTCTGGGAT 
GTTCCGCCGA CTGGATTCGA GACAACTACA CCGATGGAGT ACAAGAATAT GCAGGCGGCT 
GGACAGGTTC CACGTGGATC TGTACAGTCT GCAGTGCCCG TTGTTGGCCC ATCAGTGACT 
TGTCAATCAC GTCGTCTCTA CGTTGGAAAT ATTCCGTTCG GATGCAATGA AGAAGCTATG 
CTCGACTTTT TCAATCAACA AATGCACCTC TGTGGATTGG CTCAAGCGCC TGGAAATCCG 
ATTCTTCTGT GCCAAATAAA TTTGGACAAA AATTTCGCAT TCATAGAATT CCGTTCAATA 
GACGAAACGA CGGCCGGAAT GGCATTTGAC GGAATCAATT TCATGGGACA ACAGTTGAAA 
GTTCGACGTC CAAGAGACTA TCAACCATCT CAAAATACGT TTGATATGAA CTCTCGTATG 
CCTGTTTCAA CGATTGTCGT CGATTCGGCC AATAAGATCT TTATCGGTGG TCTGCCCAAC 
TATTTGACTG AGGATCAGGT AAAAGAGCTT TTGTGCTCAT TTGGACCGCT TAAGGCGTTC 
TCGTTGAACG TTGATTCGCA AGGAAATAGC AAAGGATACG CGTTCGCCGA ATATTTGGAC 
CCAACTCTCA CTGATCAGGC CATCGCCGGG CTCAACGGTA TGCAACTCGG CGACAAGCAG 
CTCGTCGTTC AACTTGCCTG TGCTAATCAA CAACGTCATA ACACAAATCT GCCGAACTCT 
GCGTCAGCAA TTGCCGGAAT TGATCTCTCC CAAGGAGCCG GCCGAGCCAC TGAGATCCTT 
TGTCTCATGA ATATGGTGAC TGAGGATGAG CTGAAAGCTG ATGATGAGTA CGAAGAGATT 
CTGGAGGATG TACGCGATGA ATGCTCAAAA TATGGAATTG TTCGCTCGTT GGAAATTCCA 
AGACCGTATG AGGATCATCC GGTTCCAGGT GTTGGGAAGG TTTTCGTCGA ATTCGCCTCC 
ACATCCGACT GTCAACGTGC TCAGGCTGCT CTGACCGGAC GAAAATTCGC GAATCGCACC 
GTCGTCACCT CCTACTATGA CGTCGACAAG TACCACAATC GTCAATTCTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCa340822
Clone ID Related Accession (Same CDS sequence) NM_001027796.4 , NM_001027796.3
Accession Version NM_001027796.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1491bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-04-08
Organism Caenorhabditis elegans
Product Splicing factor U2AF 65 kDa subunit
Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Jun 26, 2012 this sequence version replaced NM_001027796.2. COMPLETENESS: incomplete on both ends. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGAGTGATC ACCAGGATGG CATGAAACTT GAAGATATTG AACGCCAATT TTTGGACGTT 
GCTCAACGTG AAGGAGGTCT CGAAGCGATT CAGCCGACCA CTGGACCACT GGAAAATGAA 
GAAAATCTGA AATCATCGAC TGGTGGTGGT GGTGGAGAGG ATGATAATGA TCGGAAAAAG 
AGAAAACGAT CGAGAAGTAG AGATCGCGAT ACGAGAAGAC GCTCGAGAAG TCGTGATCGT 
GGAGAACGTC GTGGTGGTGG AGGTGGAGGT GATCGTGATC GTTCTCGATC CCGTGAACGT 
CGTCGAGGCG GTGGTGGTCG TGATGAGCCG AGACGTCGTG GAGGTGATGA TGAAGCCCGT 
TCGCGCCGTG AGCCGGAGCC CCAGAAGCCT CGTGAGCCCA AAAAGTACCG ATTCTGGGAT 
GTTCCGCCGA CTGGATTCGA GACAACTACA CCGATGGAGT ACAAGAATAT GCAGGCGGCT 
GGACAGGTTC CACGTGGATC TGTACAGTCT GCAGTGCCCG TTGTTGGCCC ATCAGTGACT 
TGTCAATCAC GTCGTCTCTA CGTTGGAAAT ATTCCGTTCG GATGCAATGA AGAAGCTATG 
CTCGACTTTT TCAATCAACA AATGCACCTC TGTGGATTGG CTCAAGCGCC TGGAAATCCG 
ATTCTTCTGT GCCAAATAAA TTTGGACAAA AATTTCGCAT TCATAGAATT CCGTTCAATA 
GACGAAACGA CGGCCGGAAT GGCATTTGAC GGAATCAATT TCATGGGACA ACAGTTGAAA 
GTTCGACGTC CAAGAGACTA TCAACCATCT CAAAATACGT TTGATATGAA CTCTCGTATG 
CCTGTTTCAA CGATTGTCGT CGATTCGGCC AATAAGATCT TTATCGGTGG TCTGCCCAAC 
TATTTGACTG AGGATCAGGT AAAAGAGCTT TTGTGCTCAT TTGGACCGCT TAAGGCGTTC 
TCGTTGAACG TTGATTCGCA AGGAAATAGC AAAGGATACG CGTTCGCCGA ATATTTGGAC 
CCAACTCTCA CTGATCAGGC CATCGCCGGG CTCAACGGTA TGCAACTCGG CGACAAGCAG 
CTCGTCGTTC AACTTGCCTG TGCTAATCAA CAACGTCATA ACACAAATCT GCCGAACTCT 
GCGTCAGCAA TTGCCGGAAT TGATCTCTCC CAAGGAGCCG GCCGAGCCAC TGAGATCCTT 
TGTCTCATGA ATATGGTGAC TGAGGATGAG CTGAAAGCTG ATGATGAGTA CGAAGAGATT 
CTGGAGGATG TACGCGATGA ATGCTCAAAA TATGGAATTG TTCGCTCGTT GGAAATTCCA 
AGACCGTATG AGGATCATCC GGTTCCAGGT GTTGGGAAGG TTTTCGTCGA ATTCGCCTCC 
ACATCCGACT GTCAACGTGC TCAGGCTGCT CTGACCGGAC GAAAATTCGC GAATCGCACC 
GTCGTCACCT CCTACTATGA CGTCGACAAG TACCACAATC GTCAATTCTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001022967.1
CDS1..1491
Translation

Target ORF information:

RefSeq Version NM_001027796.3
Organism Caenorhabditis elegans
Definition Caenorhabditis elegans Splicing factor U2AF 65 kDa subunit (uaf-1), partial mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001027796.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGAGTGATC ACCAGGATGG CATGAAACTT GAAGATATTG AACGCCAATT TTTGGACGTT 
GCTCAACGTG AAGGAGGTCT CGAAGCGATT CAGCCGACCA CTGGACCACT GGAAAATGAA 
GAAAATCTGA AATCATCGAC TGGTGGTGGT GGTGGAGAGG ATGATAATGA TCGGAAAAAG 
AGAAAACGAT CGAGAAGTAG AGATCGCGAT ACGAGAAGAC GCTCGAGAAG TCGTGATCGT 
GGAGAACGTC GTGGTGGTGG AGGTGGAGGT GATCGTGATC GTTCTCGATC CCGTGAACGT 
CGTCGAGGCG GTGGTGGTCG TGATGAGCCG AGACGTCGTG GAGGTGATGA TGAAGCCCGT 
TCGCGCCGTG AGCCGGAGCC CCAGAAGCCT CGTGAGCCCA AAAAGTACCG ATTCTGGGAT 
GTTCCGCCGA CTGGATTCGA GACAACTACA CCGATGGAGT ACAAGAATAT GCAGGCGGCT 
GGACAGGTTC CACGTGGATC TGTACAGTCT GCAGTGCCCG TTGTTGGCCC ATCAGTGACT 
TGTCAATCAC GTCGTCTCTA CGTTGGAAAT ATTCCGTTCG GATGCAATGA AGAAGCTATG 
CTCGACTTTT TCAATCAACA AATGCACCTC TGTGGATTGG CTCAAGCGCC TGGAAATCCG 
ATTCTTCTGT GCCAAATAAA TTTGGACAAA AATTTCGCAT TCATAGAATT CCGTTCAATA 
GACGAAACGA CGGCCGGAAT GGCATTTGAC GGAATCAATT TCATGGGACA ACAGTTGAAA 
GTTCGACGTC CAAGAGACTA TCAACCATCT CAAAATACGT TTGATATGAA CTCTCGTATG 
CCTGTTTCAA CGATTGTCGT CGATTCGGCC AATAAGATCT TTATCGGTGG TCTGCCCAAC 
TATTTGACTG AGGATCAGGT AAAAGAGCTT TTGTGCTCAT TTGGACCGCT TAAGGCGTTC 
TCGTTGAACG TTGATTCGCA AGGAAATAGC AAAGGATACG CGTTCGCCGA ATATTTGGAC 
CCAACTCTCA CTGATCAGGC CATCGCCGGG CTCAACGGTA TGCAACTCGG CGACAAGCAG 
CTCGTCGTTC AACTTGCCTG TGCTAATCAA CAACGTCATA ACACAAATCT GCCGAACTCT 
GCGTCAGCAA TTGCCGGAAT TGATCTCTCC CAAGGAGCCG GCCGAGCCAC TGAGATCCTT 
TGTCTCATGA ATATGGTGAC TGAGGATGAG CTGAAAGCTG ATGATGAGTA CGAAGAGATT 
CTGGAGGATG TACGCGATGA ATGCTCAAAA TATGGAATTG TTCGCTCGTT GGAAATTCCA 
AGACCGTATG AGGATCATCC GGTTCCAGGT GTTGGGAAGG TTTTCGTCGA ATTCGCCTCC 
ACATCCGACT GTCAACGTGC TCAGGCTGCT CTGACCGGAC GAAAATTCGC GAATCGCACC 
GTCGTCACCT CCTACTATGA CGTCGACAAG TACCACAATC GTCAATTCTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCa340823
Clone ID Related Accession (Same CDS sequence) NM_064925.6 , NM_001373843.1
Accession Version NM_064925.6 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 432bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-04-08
Organism Caenorhabditis elegans
Product Splicing factor U2AF 65 kDa subunit
Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Jun 26, 2012 this sequence version replaced NM_064925.5. COMPLETENESS: incomplete on both ends. 

1
61
121
181
241
301
361
421
ATGCAACTCG GCGACAAGCA GCTCGTCGTT CAACTTGCCT GTGCTAATCA ACAACGTCAT 
AACACAAATC TGCCGAACTC TGCGTCAGCA ATTGCCGGAA TTGATCTCTC CCAAGGAGCC 
GGCCGAGCCA CTGAGATCCT TTGTCTCATG AATATGGTGA CTGAGGATGA GCTGAAAGCT 
GATGATGAGT ACGAAGAGAT TCTGGAGGAT GTACGCGATG AATGCTCAAA ATATGGAATT 
GTTCGCTCGT TGGAAATTCC AAGACCGTAT GAGGATCATC CGGTTCCAGG TGTTGGGAAG 
GTTTTCGTCG AATTCGCCTC CACATCCGAC TGTCAACGTG CTCAGGCTGC TCTGACCGGA 
CGAAAATTCG CGAATCGCAC CGTCGTCACC TCCTACTATG ACGTCGACAA GTACCACAAT 
CGTCAATTCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_497326.2
CDS1..432
Translation

Target ORF information:

RefSeq Version NM_064925.6
Organism Caenorhabditis elegans
Definition Caenorhabditis elegans Splicing factor U2AF 65 kDa subunit (uaf-1), partial mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_064925.6

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGCAACTCG GCGACAAGCA GCTCGTCGTT CAACTTGCCT GTGCTAATCA ACAACGTCAT 
AACACAAATC TGCCGAACTC TGCGTCAGCA ATTGCCGGAA TTGATCTCTC CCAAGGAGCC 
GGCCGAGCCA CTGAGATCCT TTGTCTCATG AATATGGTGA CTGAGGATGA GCTGAAAGCT 
GATGATGAGT ACGAAGAGAT TCTGGAGGAT GTACGCGATG AATGCTCAAA ATATGGAATT 
GTTCGCTCGT TGGAAATTCC AAGACCGTAT GAGGATCATC CGGTTCCAGG TGTTGGGAAG 
GTTTTCGTCG AATTCGCCTC CACATCCGAC TGTCAACGTG CTCAGGCTGC TCTGACCGGA 
CGAAAATTCG CGAATCGCAC CGTCGTCACC TCCTACTATG ACGTCGACAA GTACCACAAT 
CGTCAATTCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCa340823
Clone ID Related Accession (Same CDS sequence) NM_064925.6 , NM_001373843.1
Accession Version NM_001373843.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 432bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-10-17
Organism Caenorhabditis elegans
Product Splicing factor U2AF 65 kDa subunit
Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). 

1
61
121
181
241
301
361
421
ATGCAACTCG GCGACAAGCA GCTCGTCGTT CAACTTGCCT GTGCTAATCA ACAACGTCAT 
AACACAAATC TGCCGAACTC TGCGTCAGCA ATTGCCGGAA TTGATCTCTC CCAAGGAGCC 
GGCCGAGCCA CTGAGATCCT TTGTCTCATG AATATGGTGA CTGAGGATGA GCTGAAAGCT 
GATGATGAGT ACGAAGAGAT TCTGGAGGAT GTACGCGATG AATGCTCAAA ATATGGAATT 
GTTCGCTCGT TGGAAATTCC AAGACCGTAT GAGGATCATC CGGTTCCAGG TGTTGGGAAG 
GTTTTCGTCG AATTCGCCTC CACATCCGAC TGTCAACGTG CTCAGGCTGC TCTGACCGGA 
CGAAAATTCG CGAATCGCAC CGTCGTCACC TCCTACTATG ACGTCGACAA GTACCACAAT 
CGTCAATTCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001359811.1
CDS1276..1707
Translation

Target ORF information:

RefSeq Version NM_001373843.1
Organism Caenorhabditis elegans
Definition Caenorhabditis elegans Splicing factor U2AF 65 kDa subunit (uaf-1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001373843.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGCAACTCG GCGACAAGCA GCTCGTCGTT CAACTTGCCT GTGCTAATCA ACAACGTCAT 
AACACAAATC TGCCGAACTC TGCGTCAGCA ATTGCCGGAA TTGATCTCTC CCAAGGAGCC 
GGCCGAGCCA CTGAGATCCT TTGTCTCATG AATATGGTGA CTGAGGATGA GCTGAAAGCT 
GATGATGAGT ACGAAGAGAT TCTGGAGGAT GTACGCGATG AATGCTCAAA ATATGGAATT 
GTTCGCTCGT TGGAAATTCC AAGACCGTAT GAGGATCATC CGGTTCCAGG TGTTGGGAAG 
GTTTTCGTCG AATTCGCCTC CACATCCGAC TGTCAACGTG CTCAGGCTGC TCTGACCGGA 
CGAAAATTCG CGAATCGCAC CGTCGTCACC TCCTACTATG ACGTCGACAA GTACCACAAT 
CGTCAATTCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCa340824
Clone ID Related Accession (Same CDS sequence) NM_001027798.1
Accession Version NM_001027798.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1425bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-04-08
Organism Caenorhabditis elegans
Product Splicing factor U2AF 65 kDa subunit
Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). COMPLETENESS: incomplete on both ends. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGAGTGATC ACCAGGATGG CATGAAACTT GAAGATATTG AACGCCAATT TTTGGACGTT 
GCTCAACGTG AAGGAGGTCT CGAAGCGATT CAGCCGACCA CTGGACCACT GGAAAATGAA 
GAAAATCTGA AATCATCGAC TGGTGGTGGT GGTGGAGAGG ATGATAATGA TCGTGGAGAA 
CGTCGTGGTG GTGGAGGTGG AGGTGATCGT GATCGTTCTC GATCCCGTGA ACGTCGTCGA 
GGCGGTGGTG GTCGTGATGA GCCGAGACGT CGTGGAGGTG ATGATGAAGC CCGTTCGCGC 
CGTGAGCCGG AGCCCCAGAA GCCTCGTGAG CCCAAAAAGT ACCGATTCTG GGATGTTCCG 
CCGACTGGAT TCGAGACAAC TACACCGATG GAGTACAAGA ATATGCAGGC GGCTGGACAG 
GTTCCACGTG GATCTGTACA GTCTGCAGTG CCCGTTGTTG GCCCATCAGT GACTTGTCAA 
TCACGTCGTC TCTACGTTGG AAATATTCCG TTCGGATGCA ATGAAGAAGC TATGCTCGAC 
TTTTTCAATC AACAAATGCA CCTCTGTGGA TTGGCTCAAG CGCCTGGAAA TCCGATTCTT 
CTGTGCCAAA TAAATTTGGA CAAAAATTTC GCATTCATAG AATTCCGTTC AATAGACGAA 
ACGACGGCCG GAATGGCATT TGACGGAATC AATTTCATGG GACAACAGTT GAAAGTTCGA 
CGTCCAAGAG ACTATCAACC ATCTCAAAAT ACGTTTGATA TGAACTCTCG TATGCCTGTT 
TCAACGATTG TCGTCGATTC GGCCAATAAG ATCTTTATCG GTGGTCTGCC CAACTATTTG 
ACTGAGGATC AGGTAAAAGA GCTTTTGTGC TCATTTGGAC CGCTTAAGGC GTTCTCGTTG 
AACGTTGATT CGCAAGGAAA TAGCAAAGGA TACGCGTTCG CCGAATATTT GGACCCAACT 
CTCACTGATC AGGCCATCGC CGGGCTCAAC GGTATGCAAC TCGGCGACAA GCAGCTCGTC 
GTTCAACTTG CCTGTGCTAA TCAACAACGT CATAACACAA ATCTGCCGAA CTCTGCGTCA 
GCAATTGCCG GAATTGATCT CTCCCAAGGA GCCGGCCGAG CCACTGAGAT CCTTTGTCTC 
ATGAATATGG TGACTGAGGA TGAGCTGAAA GCTGATGATG AGTACGAAGA GATTCTGGAG 
GATGTACGCG ATGAATGCTC AAAATATGGA ATTGTTCGCT CGTTGGAAAT TCCAAGACCG 
TATGAGGATC ATCCGGTTCC AGGTGTTGGG AAGGTTTTCG TCGAATTCGC CTCCACATCC 
GACTGTCAAC GTGCTCAGGC TGCTCTGACC GGACGAAAAT TCGCGAATCG CACCGTCGTC 
ACCTCCTACT ATGACGTCGA CAAGTACCAC AATCGTCAAT TCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001022969.1
CDS1..1425
Translation

Target ORF information:

RefSeq Version NM_001027798.1
Organism Caenorhabditis elegans
Definition Caenorhabditis elegans Splicing factor U2AF 65 kDa subunit (uaf-1), partial mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001027798.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGAGTGATC ACCAGGATGG CATGAAACTT GAAGATATTG AACGCCAATT TTTGGACGTT 
GCTCAACGTG AAGGAGGTCT CGAAGCGATT CAGCCGACCA CTGGACCACT GGAAAATGAA 
GAAAATCTGA AATCATCGAC TGGTGGTGGT GGTGGAGAGG ATGATAATGA TCGTGGAGAA 
CGTCGTGGTG GTGGAGGTGG AGGTGATCGT GATCGTTCTC GATCCCGTGA ACGTCGTCGA 
GGCGGTGGTG GTCGTGATGA GCCGAGACGT CGTGGAGGTG ATGATGAAGC CCGTTCGCGC 
CGTGAGCCGG AGCCCCAGAA GCCTCGTGAG CCCAAAAAGT ACCGATTCTG GGATGTTCCG 
CCGACTGGAT TCGAGACAAC TACACCGATG GAGTACAAGA ATATGCAGGC GGCTGGACAG 
GTTCCACGTG GATCTGTACA GTCTGCAGTG CCCGTTGTTG GCCCATCAGT GACTTGTCAA 
TCACGTCGTC TCTACGTTGG AAATATTCCG TTCGGATGCA ATGAAGAAGC TATGCTCGAC 
TTTTTCAATC AACAAATGCA CCTCTGTGGA TTGGCTCAAG CGCCTGGAAA TCCGATTCTT 
CTGTGCCAAA TAAATTTGGA CAAAAATTTC GCATTCATAG AATTCCGTTC AATAGACGAA 
ACGACGGCCG GAATGGCATT TGACGGAATC AATTTCATGG GACAACAGTT GAAAGTTCGA 
CGTCCAAGAG ACTATCAACC ATCTCAAAAT ACGTTTGATA TGAACTCTCG TATGCCTGTT 
TCAACGATTG TCGTCGATTC GGCCAATAAG ATCTTTATCG GTGGTCTGCC CAACTATTTG 
ACTGAGGATC AGGTAAAAGA GCTTTTGTGC TCATTTGGAC CGCTTAAGGC GTTCTCGTTG 
AACGTTGATT CGCAAGGAAA TAGCAAAGGA TACGCGTTCG CCGAATATTT GGACCCAACT 
CTCACTGATC AGGCCATCGC CGGGCTCAAC GGTATGCAAC TCGGCGACAA GCAGCTCGTC 
GTTCAACTTG CCTGTGCTAA TCAACAACGT CATAACACAA ATCTGCCGAA CTCTGCGTCA 
GCAATTGCCG GAATTGATCT CTCCCAAGGA GCCGGCCGAG CCACTGAGAT CCTTTGTCTC 
ATGAATATGG TGACTGAGGA TGAGCTGAAA GCTGATGATG AGTACGAAGA GATTCTGGAG 
GATGTACGCG ATGAATGCTC AAAATATGGA ATTGTTCGCT CGTTGGAAAT TCCAAGACCG 
TATGAGGATC ATCCGGTTCC AGGTGTTGGG AAGGTTTTCG TCGAATTCGC CTCCACATCC 
GACTGTCAAC GTGCTCAGGC TGCTCTGACC GGACGAAAAT TCGCGAATCG CACCGTCGTC 
ACCTCCTACT ATGACGTCGA CAAGTACCAC AATCGTCAAT TCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCa340825
Clone ID Related Accession (Same CDS sequence) NM_001027799.1
Accession Version NM_001027799.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1416bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-04-08
Organism Caenorhabditis elegans
Product U2 snRNP auxiliary factor large subunit
Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). COMPLETENESS: incomplete on both ends. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGAGTGATC ACCAGGATGG CATGAAACTT GAAGATATTG AACGCCAATT TTTGGACGTT 
GCTCAACGTG AAGGAGGTCT CGAAGCGATT CAGCCGACCA CTGGACCACT GGAAAATGAA 
GAAAATCTGA AATCATCGAC TGGTGGTGGT GGTGGAGAGG ATGATAATGA TCGGAAAAAG 
AGAAAACGAT CGAGAAGTAG AGATCGCGAT ACGAGAAGAC GCTCGAGAAG TCGTGATCGT 
GGAGAACGTC GTGGTGGTGG AGGTGGAGGT GATGATGAAG CCCGTTCGCG CCGTGAGCCG 
GAGCCCCAGA AGCCTCGTGA GCCCAAAAAG TACCGATTCT GGGATGTTCC GCCGACTGGA 
TTCGAGACAA CTACACCGAT GGAGTACAAG AATATGCAGG CGGCTGGACA GGTTCCACGT 
GGATCTGTAC AGTCTGCAGT GCCCGTTGTT GGCCCATCAG TGACTTGTCA ATCACGTCGT 
CTCTACGTTG GAAATATTCC GTTCGGATGC AATGAAGAAG CTATGCTCGA CTTTTTCAAT 
CAACAAATGC ACCTCTGTGG ATTGGCTCAA GCGCCTGGAA ATCCGATTCT TCTGTGCCAA 
ATAAATTTGG ACAAAAATTT CGCATTCATA GAATTCCGTT CAATAGACGA AACGACGGCC 
GGAATGGCAT TTGACGGAAT CAATTTCATG GGACAACAGT TGAAAGTTCG ACGTCCAAGA 
GACTATCAAC CATCTCAAAA TACGTTTGAT ATGAACTCTC GTATGCCTGT TTCAACGATT 
GTCGTCGATT CGGCCAATAA GATCTTTATC GGTGGTCTGC CCAACTATTT GACTGAGGAT 
CAGGTAAAAG AGCTTTTGTG CTCATTTGGA CCGCTTAAGG CGTTCTCGTT GAACGTTGAT 
TCGCAAGGAA ATAGCAAAGG ATACGCGTTC GCCGAATATT TGGACCCAAC TCTCACTGAT 
CAGGCCATCG CCGGGCTCAA CGGTATGCAA CTCGGCGACA AGCAGCTCGT CGTTCAACTT 
GCCTGTGCTA ATCAACAACG TCATAACACA AATCTGCCGA ACTCTGCGTC AGCAATTGCC 
GGAATTGATC TCTCCCAAGG AGCCGGCCGA GCCACTGAGA TCCTTTGTCT CATGAATATG 
GTGACTGAGG ATGAGCTGAA AGCTGATGAT GAGTACGAAG AGATTCTGGA GGATGTACGC 
GATGAATGCT CAAAATATGG AATTGTTCGC TCGTTGGAAA TTCCAAGACC GTATGAGGAT 
CATCCGGTTC CAGGTGTTGG GAAGGTTTTC GTCGAATTCG CCTCCACATC CGACTGTCAA 
CGTGCTCAGG CTGCTCTGAC CGGACGAAAA TTCGCGAATC GCACCGTCGT CACCTCCTAC 
TATGACGTCG ACAAGTACCA CAATCGTCAA TTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001022970.1
CDS1..1416
Translation

Target ORF information:

RefSeq Version NM_001027799.1
Organism Caenorhabditis elegans
Definition Caenorhabditis elegans U2 snRNP auxiliary factor large subunit (uaf-1), partial mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001027799.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGAGTGATC ACCAGGATGG CATGAAACTT GAAGATATTG AACGCCAATT TTTGGACGTT 
GCTCAACGTG AAGGAGGTCT CGAAGCGATT CAGCCGACCA CTGGACCACT GGAAAATGAA 
GAAAATCTGA AATCATCGAC TGGTGGTGGT GGTGGAGAGG ATGATAATGA TCGGAAAAAG 
AGAAAACGAT CGAGAAGTAG AGATCGCGAT ACGAGAAGAC GCTCGAGAAG TCGTGATCGT 
GGAGAACGTC GTGGTGGTGG AGGTGGAGGT GATGATGAAG CCCGTTCGCG CCGTGAGCCG 
GAGCCCCAGA AGCCTCGTGA GCCCAAAAAG TACCGATTCT GGGATGTTCC GCCGACTGGA 
TTCGAGACAA CTACACCGAT GGAGTACAAG AATATGCAGG CGGCTGGACA GGTTCCACGT 
GGATCTGTAC AGTCTGCAGT GCCCGTTGTT GGCCCATCAG TGACTTGTCA ATCACGTCGT 
CTCTACGTTG GAAATATTCC GTTCGGATGC AATGAAGAAG CTATGCTCGA CTTTTTCAAT 
CAACAAATGC ACCTCTGTGG ATTGGCTCAA GCGCCTGGAA ATCCGATTCT TCTGTGCCAA 
ATAAATTTGG ACAAAAATTT CGCATTCATA GAATTCCGTT CAATAGACGA AACGACGGCC 
GGAATGGCAT TTGACGGAAT CAATTTCATG GGACAACAGT TGAAAGTTCG ACGTCCAAGA 
GACTATCAAC CATCTCAAAA TACGTTTGAT ATGAACTCTC GTATGCCTGT TTCAACGATT 
GTCGTCGATT CGGCCAATAA GATCTTTATC GGTGGTCTGC CCAACTATTT GACTGAGGAT 
CAGGTAAAAG AGCTTTTGTG CTCATTTGGA CCGCTTAAGG CGTTCTCGTT GAACGTTGAT 
TCGCAAGGAA ATAGCAAAGG ATACGCGTTC GCCGAATATT TGGACCCAAC TCTCACTGAT 
CAGGCCATCG CCGGGCTCAA CGGTATGCAA CTCGGCGACA AGCAGCTCGT CGTTCAACTT 
GCCTGTGCTA ATCAACAACG TCATAACACA AATCTGCCGA ACTCTGCGTC AGCAATTGCC 
GGAATTGATC TCTCCCAAGG AGCCGGCCGA GCCACTGAGA TCCTTTGTCT CATGAATATG 
GTGACTGAGG ATGAGCTGAA AGCTGATGAT GAGTACGAAG AGATTCTGGA GGATGTACGC 
GATGAATGCT CAAAATATGG AATTGTTCGC TCGTTGGAAA TTCCAAGACC GTATGAGGAT 
CATCCGGTTC CAGGTGTTGG GAAGGTTTTC GTCGAATTCG CCTCCACATC CGACTGTCAA 
CGTGCTCAGG CTGCTCTGAC CGGACGAAAA TTCGCGAATC GCACCGTCGT CACCTCCTAC 
TATGACGTCG ACAAGTACCA CAATCGTCAA TTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)