ALAS1 cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following ALAS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ALAS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu14916 NM_199166.2
Latest version!
Homo sapiens 5'-aminolevulinate synthase 1 (ALAS1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
OHu14916 NM_001304443.1
Latest version!
Homo sapiens 5'-aminolevulinate synthase 1 (ALAS1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
OHu14916 NM_000688.6
Latest version!
Homo sapiens 5'-aminolevulinate synthase 1 (ALAS1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
OHu37039 NM_001304444.1
Latest version!
Homo sapiens 5'-aminolevulinate synthase 1 (ALAS1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
OHu56227 XM_011533477.2
Latest version!
Homo sapiens 5'-aminolevulinate synthase 1 (ALAS1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu56228 XM_011533478.2
Latest version!
Homo sapiens 5'-aminolevulinate synthase 1 (ALAS1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu93882 XM_024453393.1
Latest version!
Homo sapiens 5'-aminolevulinate synthase 1 (ALAS1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OHu93882 XM_017005873.1
Latest version!
Homo sapiens 5'-aminolevulinate synthase 1 (ALAS1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
View Old Accession Versions >>
OHu56227 XM_011533477.1 Homo sapiens 5'-aminolevulinate synthase 1 (ALAS1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu14916 NM_000688.5 Homo sapiens 5'-aminolevulinate synthase 1 (ALAS1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu14916
    Clone ID Related Accession (Same CDS sequence) NM_199166.2 , NM_000688.5 , NM_001304443.1 , NM_000688.6
    Accession Version NM_199166.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1923bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-09
    Organism Homo sapiens(human)
    Product 5-aminolevulinate synthase, nonspecific, mitochondrial isoform 1 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BM546974.1, BU173391.1, X56351.1 and AI868691.1. On Dec 15, 2011 this sequence version replaced NM_199166.1. Transcript Variant: This variant (2) lacks an alternate exon in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB063322.1, SRR1803616.10511.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGAGTG TTGTTCGCCG CTGCCCATTC TTATCCCGAG TCCCCCAGGC CTTTCTGCAG 
    AAAGCAGGCA AATCTCTGTT GTTCTATGCC CAAAACTGCC CCAAGATGAT GGAAGTTGGG
    GCCAAGCCAG CCCCTCGGGC ATTGTCCACT GCAGCAGTAC ACTACCAACA GATCAAAGAA
    ACCCCTCCGG CCAGTGAGAA AGACAAAACT GCTAAGGCCA AGGTCCAACA GACTCCTGAT
    GGATCCCAGC AGAGTCCAGA TGGCACACAG CTTCCGTCTG GACACCCCTT GCCTGCCACA
    AGCCAGGGCA CTGCAAGCAA ATGCCCTTTC CTGGCAGCAC AGATGAATCA GAGAGGCAGC
    AGTGTCTTCT GCAAAGCCAG TCTTGAGCTT CAGGAGGATG TGCAGGAAAT GAATGCCGTG
    AGGAAAGAGG TTGCTGAAAC CTCAGCAGGC CCCAGTGTGG TTAGTGTGAA AACCGATGGA
    GGGGATCCCA GTGGACTGCT GAAGAACTTC CAGGACATCA TGCAAAAGCA AAGACCAGAA
    AGAGTGTCTC ATCTTCTTCA AGATAACTTG CCAAAATCTG TTTCCACTTT TCAGTATGAT
    CGTTTCTTTG AGAAAAAAAT TGATGAGAAA AAGAATGACC ACACCTATCG AGTTTTTAAA
    ACTGTGAACC GGCGAGCACA CATCTTCCCC ATGGCAGATG ACTATTCAGA CTCCCTCATC
    ACCAAAAAGC AAGTGTCAGT CTGGTGCAGT AATGACTACC TAGGAATGAG TCGCCACCCA
    CGGGTGTGTG GGGCAGTTAT GGACACTTTG AAACAACATG GTGCTGGGGC AGGTGGTACT
    AGAAATATTT CTGGAACTAG TAAATTCCAT GTGGACTTAG AGCGGGAGCT GGCAGACCTC
    CATGGGAAAG ATGCCGCACT CTTGTTTTCC TCGTGCTTTG TGGCCAATGA CTCAACCCTC
    TTCACCCTGG CTAAGATGAT GCCAGGCTGT GAGATTTACT CTGATTCTGG GAACCATGCC
    TCCATGATCC AAGGGATTCG AAACAGCCGA GTGCCAAAGT ACATCTTCCG CCACAATGAT
    GTCAGCCACC TCAGAGAACT GCTGCAAAGA TCTGACCCCT CAGTCCCCAA GATTGTGGCA
    TTTGAAACTG TCCATTCAAT GGATGGGGCG GTGTGCCCAC TGGAAGAGCT GTGTGATGTG
    GCCCATGAGT TTGGAGCAAT CACCTTCGTG GATGAGGTCC ACGCAGTGGG GCTTTATGGG
    GCTCGAGGCG GAGGGATTGG GGATCGGGAT GGAGTCATGC CAAAAATGGA CATCATTTCT
    GGAACACTTG GCAAAGCCTT TGGTTGTGTT GGAGGGTACA TCGCCAGCAC GAGTTCTCTG
    ATTGACACCG TACGGTCCTA TGCTGCTGGC TTCATCTTCA CCACCTCTCT GCCACCCATG
    CTGCTGGCTG GAGCCCTGGA GTCTGTGCGG ATCCTGAAGA GCGCTGAGGG ACGGGTGCTT
    CGCCGCCAGC ACCAGCGCAA CGTCAAACTC ATGAGACAGA TGCTAATGGA TGCCGGCCTC
    CCTGTTGTCC ACTGCCCCAG CCACATCATC CCTGTGCGGG TTGCAGATGC TGCTAAAAAC
    ACAGAAGTCT GTGATGAACT AATGAGCAGA CATAACATCT ACGTGCAAGC AATCAATTAC
    CCTACGGTGC CCCGGGGAGA AGAGCTCCTA CGGATTGCCC CCACCCCTCA CCACACACCC
    CAGATGATGA ACTACTTCCT TGAGAATCTG CTAGTCACAT GGAAGCAAGT GGGGCTGGAA
    CTGAAGCCTC ATTCCTCAGC TGAGTGCAAC TTCTGCAGGA GGCCACTGCA TTTTGAAGTG
    ATGAGTGAAA GAGAGAAGTC CTATTTCTCA GGCTTGAGCA AGTTGGTATC TGCTCAGGCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_954635.1
    CDS206..2128
    Translation

    Target ORF information:

    RefSeq Version NM_199166.2
    Organism Homo sapiens(human)
    Definition Homo sapiens 5'-aminolevulinate synthase 1 (ALAS1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_199166.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGAGTG TTGTTCGCCG CTGCCCATTC TTATCCCGAG TCCCCCAGGC CTTTCTGCAG 
    AAAGCAGGCA AATCTCTGTT GTTCTATGCC CAAAACTGCC CCAAGATGAT GGAAGTTGGG
    GCCAAGCCAG CCCCTCGGGC ATTGTCCACT GCAGCAGTAC ACTACCAACA GATCAAAGAA
    ACCCCTCCGG CCAGTGAGAA AGACAAAACT GCTAAGGCCA AGGTCCAACA GACTCCTGAT
    GGATCCCAGC AGAGTCCAGA TGGCACACAG CTTCCGTCTG GACACCCCTT GCCTGCCACA
    AGCCAGGGCA CTGCAAGCAA ATGCCCTTTC CTGGCAGCAC AGATGAATCA GAGAGGCAGC
    AGTGTCTTCT GCAAAGCCAG TCTTGAGCTT CAGGAGGATG TGCAGGAAAT GAATGCCGTG
    AGGAAAGAGG TTGCTGAAAC CTCAGCAGGC CCCAGTGTGG TTAGTGTGAA AACCGATGGA
    GGGGATCCCA GTGGACTGCT GAAGAACTTC CAGGACATCA TGCAAAAGCA AAGACCAGAA
    AGAGTGTCTC ATCTTCTTCA AGATAACTTG CCAAAATCTG TTTCCACTTT TCAGTATGAT
    CGTTTCTTTG AGAAAAAAAT TGATGAGAAA AAGAATGACC ACACCTATCG AGTTTTTAAA
    ACTGTGAACC GGCGAGCACA CATCTTCCCC ATGGCAGATG ACTATTCAGA CTCCCTCATC
    ACCAAAAAGC AAGTGTCAGT CTGGTGCAGT AATGACTACC TAGGAATGAG TCGCCACCCA
    CGGGTGTGTG GGGCAGTTAT GGACACTTTG AAACAACATG GTGCTGGGGC AGGTGGTACT
    AGAAATATTT CTGGAACTAG TAAATTCCAT GTGGACTTAG AGCGGGAGCT GGCAGACCTC
    CATGGGAAAG ATGCCGCACT CTTGTTTTCC TCGTGCTTTG TGGCCAATGA CTCAACCCTC
    TTCACCCTGG CTAAGATGAT GCCAGGCTGT GAGATTTACT CTGATTCTGG GAACCATGCC
    TCCATGATCC AAGGGATTCG AAACAGCCGA GTGCCAAAGT ACATCTTCCG CCACAATGAT
    GTCAGCCACC TCAGAGAACT GCTGCAAAGA TCTGACCCCT CAGTCCCCAA GATTGTGGCA
    TTTGAAACTG TCCATTCAAT GGATGGGGCG GTGTGCCCAC TGGAAGAGCT GTGTGATGTG
    GCCCATGAGT TTGGAGCAAT CACCTTCGTG GATGAGGTCC ACGCAGTGGG GCTTTATGGG
    GCTCGAGGCG GAGGGATTGG GGATCGGGAT GGAGTCATGC CAAAAATGGA CATCATTTCT
    GGAACACTTG GCAAAGCCTT TGGTTGTGTT GGAGGGTACA TCGCCAGCAC GAGTTCTCTG
    ATTGACACCG TACGGTCCTA TGCTGCTGGC TTCATCTTCA CCACCTCTCT GCCACCCATG
    CTGCTGGCTG GAGCCCTGGA GTCTGTGCGG ATCCTGAAGA GCGCTGAGGG ACGGGTGCTT
    CGCCGCCAGC ACCAGCGCAA CGTCAAACTC ATGAGACAGA TGCTAATGGA TGCCGGCCTC
    CCTGTTGTCC ACTGCCCCAG CCACATCATC CCTGTGCGGG TTGCAGATGC TGCTAAAAAC
    ACAGAAGTCT GTGATGAACT AATGAGCAGA CATAACATCT ACGTGCAAGC AATCAATTAC
    CCTACGGTGC CCCGGGGAGA AGAGCTCCTA CGGATTGCCC CCACCCCTCA CCACACACCC
    CAGATGATGA ACTACTTCCT TGAGAATCTG CTAGTCACAT GGAAGCAAGT GGGGCTGGAA
    CTGAAGCCTC ATTCCTCAGC TGAGTGCAAC TTCTGCAGGA GGCCACTGCA TTTTGAAGTG
    ATGAGTGAAA GAGAGAAGTC CTATTTCTCA GGCTTGAGCA AGTTGGTATC TGCTCAGGCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14916
    Clone ID Related Accession (Same CDS sequence) NM_199166.2 , NM_000688.5 , NM_001304443.1 , NM_000688.6
    Accession Version NM_000688.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1923bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-24
    Organism Homo sapiens(human)
    Product 5-aminolevulinate synthase, nonspecific, mitochondrial isoform 1 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC097637.2, AU279391.1, BC011798.2, CD014147.1, X56351.1 and AI868691.1. On Dec 15, 2011 this sequence version replaced NM_000688.4. Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2, and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.226761.1, SRR1803614.168472.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGAGTG TTGTTCGCCG CTGCCCATTC TTATCCCGAG TCCCCCAGGC CTTTCTGCAG 
    AAAGCAGGCA AATCTCTGTT GTTCTATGCC CAAAACTGCC CCAAGATGAT GGAAGTTGGG
    GCCAAGCCAG CCCCTCGGGC ATTGTCCACT GCAGCAGTAC ACTACCAACA GATCAAAGAA
    ACCCCTCCGG CCAGTGAGAA AGACAAAACT GCTAAGGCCA AGGTCCAACA GACTCCTGAT
    GGATCCCAGC AGAGTCCAGA TGGCACACAG CTTCCGTCTG GACACCCCTT GCCTGCCACA
    AGCCAGGGCA CTGCAAGCAA ATGCCCTTTC CTGGCAGCAC AGATGAATCA GAGAGGCAGC
    AGTGTCTTCT GCAAAGCCAG TCTTGAGCTT CAGGAGGATG TGCAGGAAAT GAATGCCGTG
    AGGAAAGAGG TTGCTGAAAC CTCAGCAGGC CCCAGTGTGG TTAGTGTGAA AACCGATGGA
    GGGGATCCCA GTGGACTGCT GAAGAACTTC CAGGACATCA TGCAAAAGCA AAGACCAGAA
    AGAGTGTCTC ATCTTCTTCA AGATAACTTG CCAAAATCTG TTTCCACTTT TCAGTATGAT
    CGTTTCTTTG AGAAAAAAAT TGATGAGAAA AAGAATGACC ACACCTATCG AGTTTTTAAA
    ACTGTGAACC GGCGAGCACA CATCTTCCCC ATGGCAGATG ACTATTCAGA CTCCCTCATC
    ACCAAAAAGC AAGTGTCAGT CTGGTGCAGT AATGACTACC TAGGAATGAG TCGCCACCCA
    CGGGTGTGTG GGGCAGTTAT GGACACTTTG AAACAACATG GTGCTGGGGC AGGTGGTACT
    AGAAATATTT CTGGAACTAG TAAATTCCAT GTGGACTTAG AGCGGGAGCT GGCAGACCTC
    CATGGGAAAG ATGCCGCACT CTTGTTTTCC TCGTGCTTTG TGGCCAATGA CTCAACCCTC
    TTCACCCTGG CTAAGATGAT GCCAGGCTGT GAGATTTACT CTGATTCTGG GAACCATGCC
    TCCATGATCC AAGGGATTCG AAACAGCCGA GTGCCAAAGT ACATCTTCCG CCACAATGAT
    GTCAGCCACC TCAGAGAACT GCTGCAAAGA TCTGACCCCT CAGTCCCCAA GATTGTGGCA
    TTTGAAACTG TCCATTCAAT GGATGGGGCG GTGTGCCCAC TGGAAGAGCT GTGTGATGTG
    GCCCATGAGT TTGGAGCAAT CACCTTCGTG GATGAGGTCC ACGCAGTGGG GCTTTATGGG
    GCTCGAGGCG GAGGGATTGG GGATCGGGAT GGAGTCATGC CAAAAATGGA CATCATTTCT
    GGAACACTTG GCAAAGCCTT TGGTTGTGTT GGAGGGTACA TCGCCAGCAC GAGTTCTCTG
    ATTGACACCG TACGGTCCTA TGCTGCTGGC TTCATCTTCA CCACCTCTCT GCCACCCATG
    CTGCTGGCTG GAGCCCTGGA GTCTGTGCGG ATCCTGAAGA GCGCTGAGGG ACGGGTGCTT
    CGCCGCCAGC ACCAGCGCAA CGTCAAACTC ATGAGACAGA TGCTAATGGA TGCCGGCCTC
    CCTGTTGTCC ACTGCCCCAG CCACATCATC CCTGTGCGGG TTGCAGATGC TGCTAAAAAC
    ACAGAAGTCT GTGATGAACT AATGAGCAGA CATAACATCT ACGTGCAAGC AATCAATTAC
    CCTACGGTGC CCCGGGGAGA AGAGCTCCTA CGGATTGCCC CCACCCCTCA CCACACACCC
    CAGATGATGA ACTACTTCCT TGAGAATCTG CTAGTCACAT GGAAGCAAGT GGGGCTGGAA
    CTGAAGCCTC ATTCCTCAGC TGAGTGCAAC TTCTGCAGGA GGCCACTGCA TTTTGAAGTG
    ATGAGTGAAA GAGAGAAGTC CTATTTCTCA GGCTTGAGCA AGTTGGTATC TGCTCAGGCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000679.1
    CDS383..2305
    Translation

    Target ORF information:

    RefSeq Version NM_000688.5
    Organism Homo sapiens(human)
    Definition Homo sapiens 5'-aminolevulinate synthase 1 (ALAS1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000688.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGAGTG TTGTTCGCCG CTGCCCATTC TTATCCCGAG TCCCCCAGGC CTTTCTGCAG 
    AAAGCAGGCA AATCTCTGTT GTTCTATGCC CAAAACTGCC CCAAGATGAT GGAAGTTGGG
    GCCAAGCCAG CCCCTCGGGC ATTGTCCACT GCAGCAGTAC ACTACCAACA GATCAAAGAA
    ACCCCTCCGG CCAGTGAGAA AGACAAAACT GCTAAGGCCA AGGTCCAACA GACTCCTGAT
    GGATCCCAGC AGAGTCCAGA TGGCACACAG CTTCCGTCTG GACACCCCTT GCCTGCCACA
    AGCCAGGGCA CTGCAAGCAA ATGCCCTTTC CTGGCAGCAC AGATGAATCA GAGAGGCAGC
    AGTGTCTTCT GCAAAGCCAG TCTTGAGCTT CAGGAGGATG TGCAGGAAAT GAATGCCGTG
    AGGAAAGAGG TTGCTGAAAC CTCAGCAGGC CCCAGTGTGG TTAGTGTGAA AACCGATGGA
    GGGGATCCCA GTGGACTGCT GAAGAACTTC CAGGACATCA TGCAAAAGCA AAGACCAGAA
    AGAGTGTCTC ATCTTCTTCA AGATAACTTG CCAAAATCTG TTTCCACTTT TCAGTATGAT
    CGTTTCTTTG AGAAAAAAAT TGATGAGAAA AAGAATGACC ACACCTATCG AGTTTTTAAA
    ACTGTGAACC GGCGAGCACA CATCTTCCCC ATGGCAGATG ACTATTCAGA CTCCCTCATC
    ACCAAAAAGC AAGTGTCAGT CTGGTGCAGT AATGACTACC TAGGAATGAG TCGCCACCCA
    CGGGTGTGTG GGGCAGTTAT GGACACTTTG AAACAACATG GTGCTGGGGC AGGTGGTACT
    AGAAATATTT CTGGAACTAG TAAATTCCAT GTGGACTTAG AGCGGGAGCT GGCAGACCTC
    CATGGGAAAG ATGCCGCACT CTTGTTTTCC TCGTGCTTTG TGGCCAATGA CTCAACCCTC
    TTCACCCTGG CTAAGATGAT GCCAGGCTGT GAGATTTACT CTGATTCTGG GAACCATGCC
    TCCATGATCC AAGGGATTCG AAACAGCCGA GTGCCAAAGT ACATCTTCCG CCACAATGAT
    GTCAGCCACC TCAGAGAACT GCTGCAAAGA TCTGACCCCT CAGTCCCCAA GATTGTGGCA
    TTTGAAACTG TCCATTCAAT GGATGGGGCG GTGTGCCCAC TGGAAGAGCT GTGTGATGTG
    GCCCATGAGT TTGGAGCAAT CACCTTCGTG GATGAGGTCC ACGCAGTGGG GCTTTATGGG
    GCTCGAGGCG GAGGGATTGG GGATCGGGAT GGAGTCATGC CAAAAATGGA CATCATTTCT
    GGAACACTTG GCAAAGCCTT TGGTTGTGTT GGAGGGTACA TCGCCAGCAC GAGTTCTCTG
    ATTGACACCG TACGGTCCTA TGCTGCTGGC TTCATCTTCA CCACCTCTCT GCCACCCATG
    CTGCTGGCTG GAGCCCTGGA GTCTGTGCGG ATCCTGAAGA GCGCTGAGGG ACGGGTGCTT
    CGCCGCCAGC ACCAGCGCAA CGTCAAACTC ATGAGACAGA TGCTAATGGA TGCCGGCCTC
    CCTGTTGTCC ACTGCCCCAG CCACATCATC CCTGTGCGGG TTGCAGATGC TGCTAAAAAC
    ACAGAAGTCT GTGATGAACT AATGAGCAGA CATAACATCT ACGTGCAAGC AATCAATTAC
    CCTACGGTGC CCCGGGGAGA AGAGCTCCTA CGGATTGCCC CCACCCCTCA CCACACACCC
    CAGATGATGA ACTACTTCCT TGAGAATCTG CTAGTCACAT GGAAGCAAGT GGGGCTGGAA
    CTGAAGCCTC ATTCCTCAGC TGAGTGCAAC TTCTGCAGGA GGCCACTGCA TTTTGAAGTG
    ATGAGTGAAA GAGAGAAGTC CTATTTCTCA GGCTTGAGCA AGTTGGTATC TGCTCAGGCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14916
    Clone ID Related Accession (Same CDS sequence) NM_199166.2 , NM_000688.5 , NM_001304443.1 , NM_000688.6
    Accession Version NM_001304443.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1923bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-10
    Organism Homo sapiens(human)
    Product 5-aminolevulinate synthase, nonspecific, mitochondrial isoform 1 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BM546974.1, DA691060.1, Y00451.1, AK300993.1 and AB063322.1. On Jan 27, 2015 this sequence version replaced XM_005264945.1. Transcript Variant: This variant (3) lacks an alternate exon and uses an alternate splice site in the 5' UTR, compared to variant 1. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Y00451.1, SRR1803617.127480.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGAGTG TTGTTCGCCG CTGCCCATTC TTATCCCGAG TCCCCCAGGC CTTTCTGCAG 
    AAAGCAGGCA AATCTCTGTT GTTCTATGCC CAAAACTGCC CCAAGATGAT GGAAGTTGGG
    GCCAAGCCAG CCCCTCGGGC ATTGTCCACT GCAGCAGTAC ACTACCAACA GATCAAAGAA
    ACCCCTCCGG CCAGTGAGAA AGACAAAACT GCTAAGGCCA AGGTCCAACA GACTCCTGAT
    GGATCCCAGC AGAGTCCAGA TGGCACACAG CTTCCGTCTG GACACCCCTT GCCTGCCACA
    AGCCAGGGCA CTGCAAGCAA ATGCCCTTTC CTGGCAGCAC AGATGAATCA GAGAGGCAGC
    AGTGTCTTCT GCAAAGCCAG TCTTGAGCTT CAGGAGGATG TGCAGGAAAT GAATGCCGTG
    AGGAAAGAGG TTGCTGAAAC CTCAGCAGGC CCCAGTGTGG TTAGTGTGAA AACCGATGGA
    GGGGATCCCA GTGGACTGCT GAAGAACTTC CAGGACATCA TGCAAAAGCA AAGACCAGAA
    AGAGTGTCTC ATCTTCTTCA AGATAACTTG CCAAAATCTG TTTCCACTTT TCAGTATGAT
    CGTTTCTTTG AGAAAAAAAT TGATGAGAAA AAGAATGACC ACACCTATCG AGTTTTTAAA
    ACTGTGAACC GGCGAGCACA CATCTTCCCC ATGGCAGATG ACTATTCAGA CTCCCTCATC
    ACCAAAAAGC AAGTGTCAGT CTGGTGCAGT AATGACTACC TAGGAATGAG TCGCCACCCA
    CGGGTGTGTG GGGCAGTTAT GGACACTTTG AAACAACATG GTGCTGGGGC AGGTGGTACT
    AGAAATATTT CTGGAACTAG TAAATTCCAT GTGGACTTAG AGCGGGAGCT GGCAGACCTC
    CATGGGAAAG ATGCCGCACT CTTGTTTTCC TCGTGCTTTG TGGCCAATGA CTCAACCCTC
    TTCACCCTGG CTAAGATGAT GCCAGGCTGT GAGATTTACT CTGATTCTGG GAACCATGCC
    TCCATGATCC AAGGGATTCG AAACAGCCGA GTGCCAAAGT ACATCTTCCG CCACAATGAT
    GTCAGCCACC TCAGAGAACT GCTGCAAAGA TCTGACCCCT CAGTCCCCAA GATTGTGGCA
    TTTGAAACTG TCCATTCAAT GGATGGGGCG GTGTGCCCAC TGGAAGAGCT GTGTGATGTG
    GCCCATGAGT TTGGAGCAAT CACCTTCGTG GATGAGGTCC ACGCAGTGGG GCTTTATGGG
    GCTCGAGGCG GAGGGATTGG GGATCGGGAT GGAGTCATGC CAAAAATGGA CATCATTTCT
    GGAACACTTG GCAAAGCCTT TGGTTGTGTT GGAGGGTACA TCGCCAGCAC GAGTTCTCTG
    ATTGACACCG TACGGTCCTA TGCTGCTGGC TTCATCTTCA CCACCTCTCT GCCACCCATG
    CTGCTGGCTG GAGCCCTGGA GTCTGTGCGG ATCCTGAAGA GCGCTGAGGG ACGGGTGCTT
    CGCCGCCAGC ACCAGCGCAA CGTCAAACTC ATGAGACAGA TGCTAATGGA TGCCGGCCTC
    CCTGTTGTCC ACTGCCCCAG CCACATCATC CCTGTGCGGG TTGCAGATGC TGCTAAAAAC
    ACAGAAGTCT GTGATGAACT AATGAGCAGA CATAACATCT ACGTGCAAGC AATCAATTAC
    CCTACGGTGC CCCGGGGAGA AGAGCTCCTA CGGATTGCCC CCACCCCTCA CCACACACCC
    CAGATGATGA ACTACTTCCT TGAGAATCTG CTAGTCACAT GGAAGCAAGT GGGGCTGGAA
    CTGAAGCCTC ATTCCTCAGC TGAGTGCAAC TTCTGCAGGA GGCCACTGCA TTTTGAAGTG
    ATGAGTGAAA GAGAGAAGTC CTATTTCTCA GGCTTGAGCA AGTTGGTATC TGCTCAGGCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001291372.1
    CDS195..2117
    Translation

    Target ORF information:

    RefSeq Version NM_001304443.1
    Organism Homo sapiens(human)
    Definition Homo sapiens 5'-aminolevulinate synthase 1 (ALAS1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001304443.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGAGTG TTGTTCGCCG CTGCCCATTC TTATCCCGAG TCCCCCAGGC CTTTCTGCAG 
    AAAGCAGGCA AATCTCTGTT GTTCTATGCC CAAAACTGCC CCAAGATGAT GGAAGTTGGG
    GCCAAGCCAG CCCCTCGGGC ATTGTCCACT GCAGCAGTAC ACTACCAACA GATCAAAGAA
    ACCCCTCCGG CCAGTGAGAA AGACAAAACT GCTAAGGCCA AGGTCCAACA GACTCCTGAT
    GGATCCCAGC AGAGTCCAGA TGGCACACAG CTTCCGTCTG GACACCCCTT GCCTGCCACA
    AGCCAGGGCA CTGCAAGCAA ATGCCCTTTC CTGGCAGCAC AGATGAATCA GAGAGGCAGC
    AGTGTCTTCT GCAAAGCCAG TCTTGAGCTT CAGGAGGATG TGCAGGAAAT GAATGCCGTG
    AGGAAAGAGG TTGCTGAAAC CTCAGCAGGC CCCAGTGTGG TTAGTGTGAA AACCGATGGA
    GGGGATCCCA GTGGACTGCT GAAGAACTTC CAGGACATCA TGCAAAAGCA AAGACCAGAA
    AGAGTGTCTC ATCTTCTTCA AGATAACTTG CCAAAATCTG TTTCCACTTT TCAGTATGAT
    CGTTTCTTTG AGAAAAAAAT TGATGAGAAA AAGAATGACC ACACCTATCG AGTTTTTAAA
    ACTGTGAACC GGCGAGCACA CATCTTCCCC ATGGCAGATG ACTATTCAGA CTCCCTCATC
    ACCAAAAAGC AAGTGTCAGT CTGGTGCAGT AATGACTACC TAGGAATGAG TCGCCACCCA
    CGGGTGTGTG GGGCAGTTAT GGACACTTTG AAACAACATG GTGCTGGGGC AGGTGGTACT
    AGAAATATTT CTGGAACTAG TAAATTCCAT GTGGACTTAG AGCGGGAGCT GGCAGACCTC
    CATGGGAAAG ATGCCGCACT CTTGTTTTCC TCGTGCTTTG TGGCCAATGA CTCAACCCTC
    TTCACCCTGG CTAAGATGAT GCCAGGCTGT GAGATTTACT CTGATTCTGG GAACCATGCC
    TCCATGATCC AAGGGATTCG AAACAGCCGA GTGCCAAAGT ACATCTTCCG CCACAATGAT
    GTCAGCCACC TCAGAGAACT GCTGCAAAGA TCTGACCCCT CAGTCCCCAA GATTGTGGCA
    TTTGAAACTG TCCATTCAAT GGATGGGGCG GTGTGCCCAC TGGAAGAGCT GTGTGATGTG
    GCCCATGAGT TTGGAGCAAT CACCTTCGTG GATGAGGTCC ACGCAGTGGG GCTTTATGGG
    GCTCGAGGCG GAGGGATTGG GGATCGGGAT GGAGTCATGC CAAAAATGGA CATCATTTCT
    GGAACACTTG GCAAAGCCTT TGGTTGTGTT GGAGGGTACA TCGCCAGCAC GAGTTCTCTG
    ATTGACACCG TACGGTCCTA TGCTGCTGGC TTCATCTTCA CCACCTCTCT GCCACCCATG
    CTGCTGGCTG GAGCCCTGGA GTCTGTGCGG ATCCTGAAGA GCGCTGAGGG ACGGGTGCTT
    CGCCGCCAGC ACCAGCGCAA CGTCAAACTC ATGAGACAGA TGCTAATGGA TGCCGGCCTC
    CCTGTTGTCC ACTGCCCCAG CCACATCATC CCTGTGCGGG TTGCAGATGC TGCTAAAAAC
    ACAGAAGTCT GTGATGAACT AATGAGCAGA CATAACATCT ACGTGCAAGC AATCAATTAC
    CCTACGGTGC CCCGGGGAGA AGAGCTCCTA CGGATTGCCC CCACCCCTCA CCACACACCC
    CAGATGATGA ACTACTTCCT TGAGAATCTG CTAGTCACAT GGAAGCAAGT GGGGCTGGAA
    CTGAAGCCTC ATTCCTCAGC TGAGTGCAAC TTCTGCAGGA GGCCACTGCA TTTTGAAGTG
    ATGAGTGAAA GAGAGAAGTC CTATTTCTCA GGCTTGAGCA AGTTGGTATC TGCTCAGGCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14916
    Clone ID Related Accession (Same CDS sequence) NM_199166.2 , NM_000688.5 , NM_001304443.1 , NM_000688.6
    Accession Version NM_000688.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1923bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-26
    Organism Homo sapiens(human)
    Product 5-aminolevulinate synthase, nonspecific, mitochondrial isoform 1 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AU279391.1, BC011798.2, CD014147.1, X56351.1 and AI868691.1. On Nov 22, 2018 this sequence version replaced NM_000688.5. Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2, and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.314247.1, SRR1803615.95332.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000484952.6/ ENSP00000418779.1 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGAGTG TTGTTCGCCG CTGCCCATTC TTATCCCGAG TCCCCCAGGC CTTTCTGCAG 
    AAAGCAGGCA AATCTCTGTT GTTCTATGCC CAAAACTGCC CCAAGATGAT GGAAGTTGGG
    GCCAAGCCAG CCCCTCGGGC ATTGTCCACT GCAGCAGTAC ACTACCAACA GATCAAAGAA
    ACCCCTCCGG CCAGTGAGAA AGACAAAACT GCTAAGGCCA AGGTCCAACA GACTCCTGAT
    GGATCCCAGC AGAGTCCAGA TGGCACACAG CTTCCGTCTG GACACCCCTT GCCTGCCACA
    AGCCAGGGCA CTGCAAGCAA ATGCCCTTTC CTGGCAGCAC AGATGAATCA GAGAGGCAGC
    AGTGTCTTCT GCAAAGCCAG TCTTGAGCTT CAGGAGGATG TGCAGGAAAT GAATGCCGTG
    AGGAAAGAGG TTGCTGAAAC CTCAGCAGGC CCCAGTGTGG TTAGTGTGAA AACCGATGGA
    GGGGATCCCA GTGGACTGCT GAAGAACTTC CAGGACATCA TGCAAAAGCA AAGACCAGAA
    AGAGTGTCTC ATCTTCTTCA AGATAACTTG CCAAAATCTG TTTCCACTTT TCAGTATGAT
    CGTTTCTTTG AGAAAAAAAT TGATGAGAAA AAGAATGACC ACACCTATCG AGTTTTTAAA
    ACTGTGAACC GGCGAGCACA CATCTTCCCC ATGGCAGATG ACTATTCAGA CTCCCTCATC
    ACCAAAAAGC AAGTGTCAGT CTGGTGCAGT AATGACTACC TAGGAATGAG TCGCCACCCA
    CGGGTGTGTG GGGCAGTTAT GGACACTTTG AAACAACATG GTGCTGGGGC AGGTGGTACT
    AGAAATATTT CTGGAACTAG TAAATTCCAT GTGGACTTAG AGCGGGAGCT GGCAGACCTC
    CATGGGAAAG ATGCCGCACT CTTGTTTTCC TCGTGCTTTG TGGCCAATGA CTCAACCCTC
    TTCACCCTGG CTAAGATGAT GCCAGGCTGT GAGATTTACT CTGATTCTGG GAACCATGCC
    TCCATGATCC AAGGGATTCG AAACAGCCGA GTGCCAAAGT ACATCTTCCG CCACAATGAT
    GTCAGCCACC TCAGAGAACT GCTGCAAAGA TCTGACCCCT CAGTCCCCAA GATTGTGGCA
    TTTGAAACTG TCCATTCAAT GGATGGGGCG GTGTGCCCAC TGGAAGAGCT GTGTGATGTG
    GCCCATGAGT TTGGAGCAAT CACCTTCGTG GATGAGGTCC ACGCAGTGGG GCTTTATGGG
    GCTCGAGGCG GAGGGATTGG GGATCGGGAT GGAGTCATGC CAAAAATGGA CATCATTTCT
    GGAACACTTG GCAAAGCCTT TGGTTGTGTT GGAGGGTACA TCGCCAGCAC GAGTTCTCTG
    ATTGACACCG TACGGTCCTA TGCTGCTGGC TTCATCTTCA CCACCTCTCT GCCACCCATG
    CTGCTGGCTG GAGCCCTGGA GTCTGTGCGG ATCCTGAAGA GCGCTGAGGG ACGGGTGCTT
    CGCCGCCAGC ACCAGCGCAA CGTCAAACTC ATGAGACAGA TGCTAATGGA TGCCGGCCTC
    CCTGTTGTCC ACTGCCCCAG CCACATCATC CCTGTGCGGG TTGCAGATGC TGCTAAAAAC
    ACAGAAGTCT GTGATGAACT AATGAGCAGA CATAACATCT ACGTGCAAGC AATCAATTAC
    CCTACGGTGC CCCGGGGAGA AGAGCTCCTA CGGATTGCCC CCACCCCTCA CCACACACCC
    CAGATGATGA ACTACTTCCT TGAGAATCTG CTAGTCACAT GGAAGCAAGT GGGGCTGGAA
    CTGAAGCCTC ATTCCTCAGC TGAGTGCAAC TTCTGCAGGA GGCCACTGCA TTTTGAAGTG
    ATGAGTGAAA GAGAGAAGTC CTATTTCTCA GGCTTGAGCA AGTTGGTATC TGCTCAGGCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000679.1
    CDS306..2228
    Translation

    Target ORF information:

    RefSeq Version NM_000688.6
    Organism Homo sapiens(human)
    Definition Homo sapiens 5'-aminolevulinate synthase 1 (ALAS1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000688.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGAGTG TTGTTCGCCG CTGCCCATTC TTATCCCGAG TCCCCCAGGC CTTTCTGCAG 
    AAAGCAGGCA AATCTCTGTT GTTCTATGCC CAAAACTGCC CCAAGATGAT GGAAGTTGGG
    GCCAAGCCAG CCCCTCGGGC ATTGTCCACT GCAGCAGTAC ACTACCAACA GATCAAAGAA
    ACCCCTCCGG CCAGTGAGAA AGACAAAACT GCTAAGGCCA AGGTCCAACA GACTCCTGAT
    GGATCCCAGC AGAGTCCAGA TGGCACACAG CTTCCGTCTG GACACCCCTT GCCTGCCACA
    AGCCAGGGCA CTGCAAGCAA ATGCCCTTTC CTGGCAGCAC AGATGAATCA GAGAGGCAGC
    AGTGTCTTCT GCAAAGCCAG TCTTGAGCTT CAGGAGGATG TGCAGGAAAT GAATGCCGTG
    AGGAAAGAGG TTGCTGAAAC CTCAGCAGGC CCCAGTGTGG TTAGTGTGAA AACCGATGGA
    GGGGATCCCA GTGGACTGCT GAAGAACTTC CAGGACATCA TGCAAAAGCA AAGACCAGAA
    AGAGTGTCTC ATCTTCTTCA AGATAACTTG CCAAAATCTG TTTCCACTTT TCAGTATGAT
    CGTTTCTTTG AGAAAAAAAT TGATGAGAAA AAGAATGACC ACACCTATCG AGTTTTTAAA
    ACTGTGAACC GGCGAGCACA CATCTTCCCC ATGGCAGATG ACTATTCAGA CTCCCTCATC
    ACCAAAAAGC AAGTGTCAGT CTGGTGCAGT AATGACTACC TAGGAATGAG TCGCCACCCA
    CGGGTGTGTG GGGCAGTTAT GGACACTTTG AAACAACATG GTGCTGGGGC AGGTGGTACT
    AGAAATATTT CTGGAACTAG TAAATTCCAT GTGGACTTAG AGCGGGAGCT GGCAGACCTC
    CATGGGAAAG ATGCCGCACT CTTGTTTTCC TCGTGCTTTG TGGCCAATGA CTCAACCCTC
    TTCACCCTGG CTAAGATGAT GCCAGGCTGT GAGATTTACT CTGATTCTGG GAACCATGCC
    TCCATGATCC AAGGGATTCG AAACAGCCGA GTGCCAAAGT ACATCTTCCG CCACAATGAT
    GTCAGCCACC TCAGAGAACT GCTGCAAAGA TCTGACCCCT CAGTCCCCAA GATTGTGGCA
    TTTGAAACTG TCCATTCAAT GGATGGGGCG GTGTGCCCAC TGGAAGAGCT GTGTGATGTG
    GCCCATGAGT TTGGAGCAAT CACCTTCGTG GATGAGGTCC ACGCAGTGGG GCTTTATGGG
    GCTCGAGGCG GAGGGATTGG GGATCGGGAT GGAGTCATGC CAAAAATGGA CATCATTTCT
    GGAACACTTG GCAAAGCCTT TGGTTGTGTT GGAGGGTACA TCGCCAGCAC GAGTTCTCTG
    ATTGACACCG TACGGTCCTA TGCTGCTGGC TTCATCTTCA CCACCTCTCT GCCACCCATG
    CTGCTGGCTG GAGCCCTGGA GTCTGTGCGG ATCCTGAAGA GCGCTGAGGG ACGGGTGCTT
    CGCCGCCAGC ACCAGCGCAA CGTCAAACTC ATGAGACAGA TGCTAATGGA TGCCGGCCTC
    CCTGTTGTCC ACTGCCCCAG CCACATCATC CCTGTGCGGG TTGCAGATGC TGCTAAAAAC
    ACAGAAGTCT GTGATGAACT AATGAGCAGA CATAACATCT ACGTGCAAGC AATCAATTAC
    CCTACGGTGC CCCGGGGAGA AGAGCTCCTA CGGATTGCCC CCACCCCTCA CCACACACCC
    CAGATGATGA ACTACTTCCT TGAGAATCTG CTAGTCACAT GGAAGCAAGT GGGGCTGGAA
    CTGAAGCCTC ATTCCTCAGC TGAGTGCAAC TTCTGCAGGA GGCCACTGCA TTTTGAAGTG
    ATGAGTGAAA GAGAGAAGTC CTATTTCTCA GGCTTGAGCA AGTTGGTATC TGCTCAGGCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu37039
    Clone ID Related Accession (Same CDS sequence) NM_001304444.1
    Accession Version NM_001304444.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1974bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-09
    Organism Homo sapiens(human)
    Product 5-aminolevulinate synthase, nonspecific, mitochondrial isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BM546974.1, DA691060.1, AK300993.1 and AB063322.1. On Jan 27, 2015 this sequence version replaced XM_005264944.2. Transcript Variant: This variant (4) uses an alternate splice site in its 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is longer than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.204335.1, AK300993.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGATGAGT GGCTTCTTCT CCACCTAGAT GAGCCAGCAT ACTTCCTGAA CATGGAGAGT 
    GTTGTTCGCC GCTGCCCATT CTTATCCCGA GTCCCCCAGG CCTTTCTGCA GAAAGCAGGC
    AAATCTCTGT TGTTCTATGC CCAAAACTGC CCCAAGATGA TGGAAGTTGG GGCCAAGCCA
    GCCCCTCGGG CATTGTCCAC TGCAGCAGTA CACTACCAAC AGATCAAAGA AACCCCTCCG
    GCCAGTGAGA AAGACAAAAC TGCTAAGGCC AAGGTCCAAC AGACTCCTGA TGGATCCCAG
    CAGAGTCCAG ATGGCACACA GCTTCCGTCT GGACACCCCT TGCCTGCCAC AAGCCAGGGC
    ACTGCAAGCA AATGCCCTTT CCTGGCAGCA CAGATGAATC AGAGAGGCAG CAGTGTCTTC
    TGCAAAGCCA GTCTTGAGCT TCAGGAGGAT GTGCAGGAAA TGAATGCCGT GAGGAAAGAG
    GTTGCTGAAA CCTCAGCAGG CCCCAGTGTG GTTAGTGTGA AAACCGATGG AGGGGATCCC
    AGTGGACTGC TGAAGAACTT CCAGGACATC ATGCAAAAGC AAAGACCAGA AAGAGTGTCT
    CATCTTCTTC AAGATAACTT GCCAAAATCT GTTTCCACTT TTCAGTATGA TCGTTTCTTT
    GAGAAAAAAA TTGATGAGAA AAAGAATGAC CACACCTATC GAGTTTTTAA AACTGTGAAC
    CGGCGAGCAC ACATCTTCCC CATGGCAGAT GACTATTCAG ACTCCCTCAT CACCAAAAAG
    CAAGTGTCAG TCTGGTGCAG TAATGACTAC CTAGGAATGA GTCGCCACCC ACGGGTGTGT
    GGGGCAGTTA TGGACACTTT GAAACAACAT GGTGCTGGGG CAGGTGGTAC TAGAAATATT
    TCTGGAACTA GTAAATTCCA TGTGGACTTA GAGCGGGAGC TGGCAGACCT CCATGGGAAA
    GATGCCGCAC TCTTGTTTTC CTCGTGCTTT GTGGCCAATG ACTCAACCCT CTTCACCCTG
    GCTAAGATGA TGCCAGGCTG TGAGATTTAC TCTGATTCTG GGAACCATGC CTCCATGATC
    CAAGGGATTC GAAACAGCCG AGTGCCAAAG TACATCTTCC GCCACAATGA TGTCAGCCAC
    CTCAGAGAAC TGCTGCAAAG ATCTGACCCC TCAGTCCCCA AGATTGTGGC ATTTGAAACT
    GTCCATTCAA TGGATGGGGC GGTGTGCCCA CTGGAAGAGC TGTGTGATGT GGCCCATGAG
    TTTGGAGCAA TCACCTTCGT GGATGAGGTC CACGCAGTGG GGCTTTATGG GGCTCGAGGC
    GGAGGGATTG GGGATCGGGA TGGAGTCATG CCAAAAATGG ACATCATTTC TGGAACACTT
    GGCAAAGCCT TTGGTTGTGT TGGAGGGTAC ATCGCCAGCA CGAGTTCTCT GATTGACACC
    GTACGGTCCT ATGCTGCTGG CTTCATCTTC ACCACCTCTC TGCCACCCAT GCTGCTGGCT
    GGAGCCCTGG AGTCTGTGCG GATCCTGAAG AGCGCTGAGG GACGGGTGCT TCGCCGCCAG
    CACCAGCGCA ACGTCAAACT CATGAGACAG ATGCTAATGG ATGCCGGCCT CCCTGTTGTC
    CACTGCCCCA GCCACATCAT CCCTGTGCGG GTTGCAGATG CTGCTAAAAA CACAGAAGTC
    TGTGATGAAC TAATGAGCAG ACATAACATC TACGTGCAAG CAATCAATTA CCCTACGGTG
    CCCCGGGGAG AAGAGCTCCT ACGGATTGCC CCCACCCCTC ACCACACACC CCAGATGATG
    AACTACTTCC TTGAGAATCT GCTAGTCACA TGGAAGCAAG TGGGGCTGGA ACTGAAGCCT
    CATTCCTCAG CTGAGTGCAA CTTCTGCAGG AGGCCACTGC ATTTTGAAGT GATGAGTGAA
    AGAGAGAAGT CCTATTTCTC AGGCTTGAGC AAGTTGGTAT CTGCTCAGGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001291373.1
    CDS321..2294
    Translation

    Target ORF information:

    RefSeq Version NM_001304444.1
    Organism Homo sapiens(human)
    Definition Homo sapiens 5'-aminolevulinate synthase 1 (ALAS1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001304444.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGATGAGT GGCTTCTTCT CCACCTAGAT GAGCCAGCAT ACTTCCTGAA CATGGAGAGT 
    GTTGTTCGCC GCTGCCCATT CTTATCCCGA GTCCCCCAGG CCTTTCTGCA GAAAGCAGGC
    AAATCTCTGT TGTTCTATGC CCAAAACTGC CCCAAGATGA TGGAAGTTGG GGCCAAGCCA
    GCCCCTCGGG CATTGTCCAC TGCAGCAGTA CACTACCAAC AGATCAAAGA AACCCCTCCG
    GCCAGTGAGA AAGACAAAAC TGCTAAGGCC AAGGTCCAAC AGACTCCTGA TGGATCCCAG
    CAGAGTCCAG ATGGCACACA GCTTCCGTCT GGACACCCCT TGCCTGCCAC AAGCCAGGGC
    ACTGCAAGCA AATGCCCTTT CCTGGCAGCA CAGATGAATC AGAGAGGCAG CAGTGTCTTC
    TGCAAAGCCA GTCTTGAGCT TCAGGAGGAT GTGCAGGAAA TGAATGCCGT GAGGAAAGAG
    GTTGCTGAAA CCTCAGCAGG CCCCAGTGTG GTTAGTGTGA AAACCGATGG AGGGGATCCC
    AGTGGACTGC TGAAGAACTT CCAGGACATC ATGCAAAAGC AAAGACCAGA AAGAGTGTCT
    CATCTTCTTC AAGATAACTT GCCAAAATCT GTTTCCACTT TTCAGTATGA TCGTTTCTTT
    GAGAAAAAAA TTGATGAGAA AAAGAATGAC CACACCTATC GAGTTTTTAA AACTGTGAAC
    CGGCGAGCAC ACATCTTCCC CATGGCAGAT GACTATTCAG ACTCCCTCAT CACCAAAAAG
    CAAGTGTCAG TCTGGTGCAG TAATGACTAC CTAGGAATGA GTCGCCACCC ACGGGTGTGT
    GGGGCAGTTA TGGACACTTT GAAACAACAT GGTGCTGGGG CAGGTGGTAC TAGAAATATT
    TCTGGAACTA GTAAATTCCA TGTGGACTTA GAGCGGGAGC TGGCAGACCT CCATGGGAAA
    GATGCCGCAC TCTTGTTTTC CTCGTGCTTT GTGGCCAATG ACTCAACCCT CTTCACCCTG
    GCTAAGATGA TGCCAGGCTG TGAGATTTAC TCTGATTCTG GGAACCATGC CTCCATGATC
    CAAGGGATTC GAAACAGCCG AGTGCCAAAG TACATCTTCC GCCACAATGA TGTCAGCCAC
    CTCAGAGAAC TGCTGCAAAG ATCTGACCCC TCAGTCCCCA AGATTGTGGC ATTTGAAACT
    GTCCATTCAA TGGATGGGGC GGTGTGCCCA CTGGAAGAGC TGTGTGATGT GGCCCATGAG
    TTTGGAGCAA TCACCTTCGT GGATGAGGTC CACGCAGTGG GGCTTTATGG GGCTCGAGGC
    GGAGGGATTG GGGATCGGGA TGGAGTCATG CCAAAAATGG ACATCATTTC TGGAACACTT
    GGCAAAGCCT TTGGTTGTGT TGGAGGGTAC ATCGCCAGCA CGAGTTCTCT GATTGACACC
    GTACGGTCCT ATGCTGCTGG CTTCATCTTC ACCACCTCTC TGCCACCCAT GCTGCTGGCT
    GGAGCCCTGG AGTCTGTGCG GATCCTGAAG AGCGCTGAGG GACGGGTGCT TCGCCGCCAG
    CACCAGCGCA ACGTCAAACT CATGAGACAG ATGCTAATGG ATGCCGGCCT CCCTGTTGTC
    CACTGCCCCA GCCACATCAT CCCTGTGCGG GTTGCAGATG CTGCTAAAAA CACAGAAGTC
    TGTGATGAAC TAATGAGCAG ACATAACATC TACGTGCAAG CAATCAATTA CCCTACGGTG
    CCCCGGGGAG AAGAGCTCCT ACGGATTGCC CCCACCCCTC ACCACACACC CCAGATGATG
    AACTACTTCC TTGAGAATCT GCTAGTCACA TGGAAGCAAG TGGGGCTGGA ACTGAAGCCT
    CATTCCTCAG CTGAGTGCAA CTTCTGCAGG AGGCCACTGC ATTTTGAAGT GATGAGTGAA
    AGAGAGAAGT CCTATTTCTC AGGCTTGAGC AAGTTGGTAT CTGCTCAGGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu56227
    Clone ID Related Accession (Same CDS sequence) XM_011533477.1 , XM_011533477.2
    Accession Version XM_011533477.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1836bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product 5-aminolevulinate synthase, nonspecific, mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGATGAGT GGCTTCTTCT CCACCTAGAT GAGCCAGCAT ACTTCCTGAA CATGGAGAGT 
    GTTGTTCGCC GCTGCCCATT CTTATCCCGA GTCCCCCAGG CCTTTCTGCA GAAAGCAGGC
    AAATCTCTGT TGTTCTATGC CCAAAACTGC CCCAAGATGA TGGAAGTTGG GGCCAAGCCA
    GCCCCTCGGG CATTGTCCAC TGCAGCAGTA CACTACCAAC AGATCAAAGA AACCCCTCCG
    GCCAGTGAGA AAGACAAAAC TGCTAAGGCC AAGGTCCAAC AGACTCCTGA TGGATCCCAG
    CAGAGTCCAG ATGGCACACA GCTTCCGTCT GGACACCCCT TGCCTGCCAC AAGCCAGGGC
    ACTGCAAGCA AATGCCCTTT CCTGGCAGCA CAGATGAATC AGAGAGGCAG CAGTGTCTTC
    TGCAAAGCCA GTCTTGAGCT TCAGGAGGAT GTGCAGGAAA TGAATGCCGT GAGGAAAGAG
    GTTGCTGAAA CCTCAGCAGG CCCCAGTGTG GTTAGTGTGA AAACCGATGG AGGGGATCCC
    AGTGGACTGC TGAAGAACTT CCAGGACATC ATGCAAAAGC AAAGACCAGA AAGAGTGTCT
    CATCTTCTTC AAGATAACTT GCCAAAATCT GTTTCCACTT TTCAGTATGA TCGTTTCTTT
    GAGAAAAAAA TTGATGAGAA AAAGAATGAC CACACCTATC GAGTTTTTAA AACTGTGAAC
    CGGCGAGCAC ACATCTTCCC CATGGCAGAT GACTATTCAG ACTCCCTCAT CACCAAAAAG
    CAAGTGTCAG TCTGGTGCAG TAATGACTAC CTAGGAATGA GTCGCCACCC ACGGGTGTGT
    GGGGCAGTTA TGGACACTTT GAAACAACAT GGTGCTGGGG CAGGTGGTAC TAGAAATATT
    TCTGGAACTA GTAAATTCCA TGTGGACTTA GAGCGGGAGC TGGCAGACCT CCATGGGAAA
    GATGCCGCAC TCTTGTTTTC CTCGTGCTTT GTGGCCAATG ACTCAACCCT CTTCACCCTG
    GCTAAGATGA TGCCAGGCTG TGAGATTTAC TCTGATTCTG GGAACCATGC CTCCATGATC
    CAAGGGATTC GAAACAGCCG AGTGCCAAAG TACATCTTCC GCCACAATGA TGTCAGCCAC
    CTCAGAGAAC TGCTGCAAAG ATCTGACCCC TCAGTCCCCA AGATTGTGGC ATTTGAAACT
    GTCCATTCAA TGGATGGGGC GGTGTGCCCA CTGGAAGAGC TGTGTGATGT GGCCCATGAG
    TTTGGAGCAA TCACCTTCGT GGATGAGGTC CACGCAGTGG GGCTTTATGG GGCTCGAGGC
    GGAGGGATTG GGGATCGGGA TGGAGTCATG CCAAAAATGG ACATCATTTC TGGAACACTT
    GGCAAAGCCT TTGGTTGTGT TGGAGGGTAC ATCGCCAGCA CGAGTTCTCT GATTGACACC
    GTACGGTCCT ATGCTGCTGG CTTCATCTTC ACCACCTCTC TGCCACCCAT GCTGCTGGCT
    GGAGCCCTGG AGTCTGTGCG GATCCTGAAG AGCGCTGAGG GACGGGTGCT TCGCCGCCAG
    CACCAGCGCA ACGTCAAACT CATGAGACAG ATGCTAATGG ATGCCGGCCT CCCTGTTGTC
    CACTGCCCCA GCCACATCAT CCCTGTGCGG GTTGCAGATG CTGCTAAAAA CACAGAAGTC
    TGTGATGAAC TAATGAGCAG ACATAACATC TACGTGCAAG CAATCAATTA CCCTACGGTG
    CCCCGGGGAG AAGAGCTCCT ACGGATTGCC CCCACCCCTC ACCACACACC CCAGATGATG
    AACTACTTCC TTGTTTCTCC TCAGAGAATC TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011531779.1
    CDS280..2115
    Translation

    Target ORF information:

    RefSeq Version XM_011533477.1
    Organism Homo sapiens(human)
    Definition Homo sapiens 5'-aminolevulinate synthase 1 (ALAS1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011533477.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGATGAGT GGCTTCTTCT CCACCTAGAT GAGCCAGCAT ACTTCCTGAA CATGGAGAGT 
    GTTGTTCGCC GCTGCCCATT CTTATCCCGA GTCCCCCAGG CCTTTCTGCA GAAAGCAGGC
    AAATCTCTGT TGTTCTATGC CCAAAACTGC CCCAAGATGA TGGAAGTTGG GGCCAAGCCA
    GCCCCTCGGG CATTGTCCAC TGCAGCAGTA CACTACCAAC AGATCAAAGA AACCCCTCCG
    GCCAGTGAGA AAGACAAAAC TGCTAAGGCC AAGGTCCAAC AGACTCCTGA TGGATCCCAG
    CAGAGTCCAG ATGGCACACA GCTTCCGTCT GGACACCCCT TGCCTGCCAC AAGCCAGGGC
    ACTGCAAGCA AATGCCCTTT CCTGGCAGCA CAGATGAATC AGAGAGGCAG CAGTGTCTTC
    TGCAAAGCCA GTCTTGAGCT TCAGGAGGAT GTGCAGGAAA TGAATGCCGT GAGGAAAGAG
    GTTGCTGAAA CCTCAGCAGG CCCCAGTGTG GTTAGTGTGA AAACCGATGG AGGGGATCCC
    AGTGGACTGC TGAAGAACTT CCAGGACATC ATGCAAAAGC AAAGACCAGA AAGAGTGTCT
    CATCTTCTTC AAGATAACTT GCCAAAATCT GTTTCCACTT TTCAGTATGA TCGTTTCTTT
    GAGAAAAAAA TTGATGAGAA AAAGAATGAC CACACCTATC GAGTTTTTAA AACTGTGAAC
    CGGCGAGCAC ACATCTTCCC CATGGCAGAT GACTATTCAG ACTCCCTCAT CACCAAAAAG
    CAAGTGTCAG TCTGGTGCAG TAATGACTAC CTAGGAATGA GTCGCCACCC ACGGGTGTGT
    GGGGCAGTTA TGGACACTTT GAAACAACAT GGTGCTGGGG CAGGTGGTAC TAGAAATATT
    TCTGGAACTA GTAAATTCCA TGTGGACTTA GAGCGGGAGC TGGCAGACCT CCATGGGAAA
    GATGCCGCAC TCTTGTTTTC CTCGTGCTTT GTGGCCAATG ACTCAACCCT CTTCACCCTG
    GCTAAGATGA TGCCAGGCTG TGAGATTTAC TCTGATTCTG GGAACCATGC CTCCATGATC
    CAAGGGATTC GAAACAGCCG AGTGCCAAAG TACATCTTCC GCCACAATGA TGTCAGCCAC
    CTCAGAGAAC TGCTGCAAAG ATCTGACCCC TCAGTCCCCA AGATTGTGGC ATTTGAAACT
    GTCCATTCAA TGGATGGGGC GGTGTGCCCA CTGGAAGAGC TGTGTGATGT GGCCCATGAG
    TTTGGAGCAA TCACCTTCGT GGATGAGGTC CACGCAGTGG GGCTTTATGG GGCTCGAGGC
    GGAGGGATTG GGGATCGGGA TGGAGTCATG CCAAAAATGG ACATCATTTC TGGAACACTT
    GGCAAAGCCT TTGGTTGTGT TGGAGGGTAC ATCGCCAGCA CGAGTTCTCT GATTGACACC
    GTACGGTCCT ATGCTGCTGG CTTCATCTTC ACCACCTCTC TGCCACCCAT GCTGCTGGCT
    GGAGCCCTGG AGTCTGTGCG GATCCTGAAG AGCGCTGAGG GACGGGTGCT TCGCCGCCAG
    CACCAGCGCA ACGTCAAACT CATGAGACAG ATGCTAATGG ATGCCGGCCT CCCTGTTGTC
    CACTGCCCCA GCCACATCAT CCCTGTGCGG GTTGCAGATG CTGCTAAAAA CACAGAAGTC
    TGTGATGAAC TAATGAGCAG ACATAACATC TACGTGCAAG CAATCAATTA CCCTACGGTG
    CCCCGGGGAG AAGAGCTCCT ACGGATTGCC CCCACCCCTC ACCACACACC CCAGATGATG
    AACTACTTCC TTGTTTCTCC TCAGAGAATC TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu56227
    Clone ID Related Accession (Same CDS sequence) XM_011533477.1 , XM_011533477.2
    Accession Version XM_011533477.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1836bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product 5-aminolevulinate synthase, nonspecific, mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011533477.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGATGAGT GGCTTCTTCT CCACCTAGAT GAGCCAGCAT ACTTCCTGAA CATGGAGAGT 
    GTTGTTCGCC GCTGCCCATT CTTATCCCGA GTCCCCCAGG CCTTTCTGCA GAAAGCAGGC
    AAATCTCTGT TGTTCTATGC CCAAAACTGC CCCAAGATGA TGGAAGTTGG GGCCAAGCCA
    GCCCCTCGGG CATTGTCCAC TGCAGCAGTA CACTACCAAC AGATCAAAGA AACCCCTCCG
    GCCAGTGAGA AAGACAAAAC TGCTAAGGCC AAGGTCCAAC AGACTCCTGA TGGATCCCAG
    CAGAGTCCAG ATGGCACACA GCTTCCGTCT GGACACCCCT TGCCTGCCAC AAGCCAGGGC
    ACTGCAAGCA AATGCCCTTT CCTGGCAGCA CAGATGAATC AGAGAGGCAG CAGTGTCTTC
    TGCAAAGCCA GTCTTGAGCT TCAGGAGGAT GTGCAGGAAA TGAATGCCGT GAGGAAAGAG
    GTTGCTGAAA CCTCAGCAGG CCCCAGTGTG GTTAGTGTGA AAACCGATGG AGGGGATCCC
    AGTGGACTGC TGAAGAACTT CCAGGACATC ATGCAAAAGC AAAGACCAGA AAGAGTGTCT
    CATCTTCTTC AAGATAACTT GCCAAAATCT GTTTCCACTT TTCAGTATGA TCGTTTCTTT
    GAGAAAAAAA TTGATGAGAA AAAGAATGAC CACACCTATC GAGTTTTTAA AACTGTGAAC
    CGGCGAGCAC ACATCTTCCC CATGGCAGAT GACTATTCAG ACTCCCTCAT CACCAAAAAG
    CAAGTGTCAG TCTGGTGCAG TAATGACTAC CTAGGAATGA GTCGCCACCC ACGGGTGTGT
    GGGGCAGTTA TGGACACTTT GAAACAACAT GGTGCTGGGG CAGGTGGTAC TAGAAATATT
    TCTGGAACTA GTAAATTCCA TGTGGACTTA GAGCGGGAGC TGGCAGACCT CCATGGGAAA
    GATGCCGCAC TCTTGTTTTC CTCGTGCTTT GTGGCCAATG ACTCAACCCT CTTCACCCTG
    GCTAAGATGA TGCCAGGCTG TGAGATTTAC TCTGATTCTG GGAACCATGC CTCCATGATC
    CAAGGGATTC GAAACAGCCG AGTGCCAAAG TACATCTTCC GCCACAATGA TGTCAGCCAC
    CTCAGAGAAC TGCTGCAAAG ATCTGACCCC TCAGTCCCCA AGATTGTGGC ATTTGAAACT
    GTCCATTCAA TGGATGGGGC GGTGTGCCCA CTGGAAGAGC TGTGTGATGT GGCCCATGAG
    TTTGGAGCAA TCACCTTCGT GGATGAGGTC CACGCAGTGG GGCTTTATGG GGCTCGAGGC
    GGAGGGATTG GGGATCGGGA TGGAGTCATG CCAAAAATGG ACATCATTTC TGGAACACTT
    GGCAAAGCCT TTGGTTGTGT TGGAGGGTAC ATCGCCAGCA CGAGTTCTCT GATTGACACC
    GTACGGTCCT ATGCTGCTGG CTTCATCTTC ACCACCTCTC TGCCACCCAT GCTGCTGGCT
    GGAGCCCTGG AGTCTGTGCG GATCCTGAAG AGCGCTGAGG GACGGGTGCT TCGCCGCCAG
    CACCAGCGCA ACGTCAAACT CATGAGACAG ATGCTAATGG ATGCCGGCCT CCCTGTTGTC
    CACTGCCCCA GCCACATCAT CCCTGTGCGG GTTGCAGATG CTGCTAAAAA CACAGAAGTC
    TGTGATGAAC TAATGAGCAG ACATAACATC TACGTGCAAG CAATCAATTA CCCTACGGTG
    CCCCGGGGAG AAGAGCTCCT ACGGATTGCC CCCACCCCTC ACCACACACC CCAGATGATG
    AACTACTTCC TTGTTTCTCC TCAGAGAATC TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011531779.1
    CDS239..2074
    Translation

    Target ORF information:

    RefSeq Version XM_011533477.2
    Organism Homo sapiens(human)
    Definition Homo sapiens 5'-aminolevulinate synthase 1 (ALAS1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011533477.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGATGAGT GGCTTCTTCT CCACCTAGAT GAGCCAGCAT ACTTCCTGAA CATGGAGAGT 
    GTTGTTCGCC GCTGCCCATT CTTATCCCGA GTCCCCCAGG CCTTTCTGCA GAAAGCAGGC
    AAATCTCTGT TGTTCTATGC CCAAAACTGC CCCAAGATGA TGGAAGTTGG GGCCAAGCCA
    GCCCCTCGGG CATTGTCCAC TGCAGCAGTA CACTACCAAC AGATCAAAGA AACCCCTCCG
    GCCAGTGAGA AAGACAAAAC TGCTAAGGCC AAGGTCCAAC AGACTCCTGA TGGATCCCAG
    CAGAGTCCAG ATGGCACACA GCTTCCGTCT GGACACCCCT TGCCTGCCAC AAGCCAGGGC
    ACTGCAAGCA AATGCCCTTT CCTGGCAGCA CAGATGAATC AGAGAGGCAG CAGTGTCTTC
    TGCAAAGCCA GTCTTGAGCT TCAGGAGGAT GTGCAGGAAA TGAATGCCGT GAGGAAAGAG
    GTTGCTGAAA CCTCAGCAGG CCCCAGTGTG GTTAGTGTGA AAACCGATGG AGGGGATCCC
    AGTGGACTGC TGAAGAACTT CCAGGACATC ATGCAAAAGC AAAGACCAGA AAGAGTGTCT
    CATCTTCTTC AAGATAACTT GCCAAAATCT GTTTCCACTT TTCAGTATGA TCGTTTCTTT
    GAGAAAAAAA TTGATGAGAA AAAGAATGAC CACACCTATC GAGTTTTTAA AACTGTGAAC
    CGGCGAGCAC ACATCTTCCC CATGGCAGAT GACTATTCAG ACTCCCTCAT CACCAAAAAG
    CAAGTGTCAG TCTGGTGCAG TAATGACTAC CTAGGAATGA GTCGCCACCC ACGGGTGTGT
    GGGGCAGTTA TGGACACTTT GAAACAACAT GGTGCTGGGG CAGGTGGTAC TAGAAATATT
    TCTGGAACTA GTAAATTCCA TGTGGACTTA GAGCGGGAGC TGGCAGACCT CCATGGGAAA
    GATGCCGCAC TCTTGTTTTC CTCGTGCTTT GTGGCCAATG ACTCAACCCT CTTCACCCTG
    GCTAAGATGA TGCCAGGCTG TGAGATTTAC TCTGATTCTG GGAACCATGC CTCCATGATC
    CAAGGGATTC GAAACAGCCG AGTGCCAAAG TACATCTTCC GCCACAATGA TGTCAGCCAC
    CTCAGAGAAC TGCTGCAAAG ATCTGACCCC TCAGTCCCCA AGATTGTGGC ATTTGAAACT
    GTCCATTCAA TGGATGGGGC GGTGTGCCCA CTGGAAGAGC TGTGTGATGT GGCCCATGAG
    TTTGGAGCAA TCACCTTCGT GGATGAGGTC CACGCAGTGG GGCTTTATGG GGCTCGAGGC
    GGAGGGATTG GGGATCGGGA TGGAGTCATG CCAAAAATGG ACATCATTTC TGGAACACTT
    GGCAAAGCCT TTGGTTGTGT TGGAGGGTAC ATCGCCAGCA CGAGTTCTCT GATTGACACC
    GTACGGTCCT ATGCTGCTGG CTTCATCTTC ACCACCTCTC TGCCACCCAT GCTGCTGGCT
    GGAGCCCTGG AGTCTGTGCG GATCCTGAAG AGCGCTGAGG GACGGGTGCT TCGCCGCCAG
    CACCAGCGCA ACGTCAAACT CATGAGACAG ATGCTAATGG ATGCCGGCCT CCCTGTTGTC
    CACTGCCCCA GCCACATCAT CCCTGTGCGG GTTGCAGATG CTGCTAAAAA CACAGAAGTC
    TGTGATGAAC TAATGAGCAG ACATAACATC TACGTGCAAG CAATCAATTA CCCTACGGTG
    CCCCGGGGAG AAGAGCTCCT ACGGATTGCC CCCACCCCTC ACCACACACC CCAGATGATG
    AACTACTTCC TTGTTTCTCC TCAGAGAATC TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu56228
    Clone ID Related Accession (Same CDS sequence) XM_011533478.2
    Accession Version XM_011533478.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1809bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product 5-aminolevulinate synthase, nonspecific, mitochondrial isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011533478.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGATGAGT GGCTTCTTCT CCACCTAGAT GAGCCAGCAT ACTTCCTGAA CATGGAGAGT 
    GTTGTTCGCC GCTGCCCATT CTTATCCCGA GTCCCCCAGG CCTTTCTGCA GAAAGCAGGC
    AAATCTCTGT TGTTCTATGC CCAAAACTGC CCCAAGATGA TGGAAGTTGG GGCCAAGCCA
    GCCCCTCGGG CATTGTCCAC TGCAGCAGTA CACTACCAAC AGATCAAAGA AACCCCTCCG
    GCCAGTGAGA AAGACAAAAC TGCTAAGGCC AAGGTCCAAC AGACTCCTGA TGGATCCCAG
    CAGAGTCCAG ATGGCACACA GCTTCCGTCT GGACACCCCT TGCCTGCCAC AAGCCAGGGC
    ACTGCAAGCA AATGCCCTTT CCTGGCAGCA CAGATGAATC AGAGAGGCAG CAGTGTCTTC
    TGCAAAGCCA GTCTTGAGCT TCAGGAGGAT GTGCAGGAAA TGAATGCCGT GAGGAAAGAG
    GTTGCTGAAA CCTCAGCAGG CCCCAGTGTG GTTAGTGTGA AAACCGATGG AGGGGATCCC
    AGTGGACTGC TGAAGAACTT CCAGGACATC ATGCAAAAGC AAAGACCAGA AAGAGTGTCT
    CATCTTCTTC AAGATAACTT GCCAAAATCT GTTTCCACTT TTCAGTATGA TCGTTTCTTT
    GAGAAAAAAA TTGATGAGAA AAAGAATGAC CACACCTATC GAGTTTTTAA AACTGTGAAC
    CGGCGAGCAC ACATCTTCCC CATGGCAGAT GACTATTCAG ACTCCCTCAT CACCAAAAAG
    CAAGTGTCAG TCTGGTGCAG TAATGACTAC CTAGGAATGA GTCGCCACCC ACGGGTGTGT
    GGGGCAGTTA TGGACACTTT GAAACAACAT GGTGCTGGGG CAGGTGGTAC TAGAAATATT
    TCTGGAACTA GTAAATTCCA TGTGGACTTA GAGCGGGAGC TGGCAGACCT CCATGGGAAA
    GATGCCGCAC TCTTGTTTTC CTCGTGCTTT GTGGCCAATG ACTCAACCCT CTTCACCCTG
    GCTAAGATGA TGCCAGGCTG TGAGATTTAC TCTGATTCTG GGAACCATGC CTCCATGATC
    CAAGGGATTC GAAACAGCCG AGTGCCAAAG TACATCTTCC GCCACAATGA TGTCAGCCAC
    CTCAGAGAAC TGCTGCAAAG ATCTGACCCC TCAGTCCCCA AGATTGTGGC ATTTGAAACT
    GTCCATTCAA TGGATGGCAA AGCCTTTGGT TGTGTTGGAG GGTACATCGC CAGCACGAGT
    TCTCTGATTG ACACCGTACG GTCCTATGCT GCTGGCTTCA TCTTCACCAC CTCTCTGCCA
    CCCATGCTGC TGGCTGGAGC CCTGGAGTCT GTGCGGATCC TGAAGAGCGC TGAGGGACGG
    GTGCTTCGCC GCCAGCACCA GCGCAACGTC AAACTCATGA GACAGATGCT AATGGATGCC
    GGCCTCCCTG TTGTCCACTG CCCCAGCCAC ATCATCCCTG TGCGGGTTGC AGATGCTGCT
    AAAAACACAG AAGTCTGTGA TGAACTAATG AGCAGACATA ACATCTACGT GCAAGCAATC
    AATTACCCTA CGGTGCCCCG GGGAGAAGAG CTCCTACGGA TTGCCCCCAC CCCTCACCAC
    ACACCCCAGA TGATGAACTA CTTCCTTGAG AATCTGCTAG TCACATGGAA GCAAGTGGGG
    CTGGAACTGA AGCCTCATTC CTCAGCTGAG TGCAACTTCT GCAGGAGGCC ACTGCATTTT
    GAAGTGATGA GTGAAAGAGA GAAGTCCTAT TTCTCAGGCT TGAGCAAGTT GGTATCTGCT
    CAGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011531780.1
    CDS280..2088
    Translation

    Target ORF information:

    RefSeq Version XM_011533478.2
    Organism Homo sapiens(human)
    Definition Homo sapiens 5'-aminolevulinate synthase 1 (ALAS1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011533478.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGATGAGT GGCTTCTTCT CCACCTAGAT GAGCCAGCAT ACTTCCTGAA CATGGAGAGT 
    GTTGTTCGCC GCTGCCCATT CTTATCCCGA GTCCCCCAGG CCTTTCTGCA GAAAGCAGGC
    AAATCTCTGT TGTTCTATGC CCAAAACTGC CCCAAGATGA TGGAAGTTGG GGCCAAGCCA
    GCCCCTCGGG CATTGTCCAC TGCAGCAGTA CACTACCAAC AGATCAAAGA AACCCCTCCG
    GCCAGTGAGA AAGACAAAAC TGCTAAGGCC AAGGTCCAAC AGACTCCTGA TGGATCCCAG
    CAGAGTCCAG ATGGCACACA GCTTCCGTCT GGACACCCCT TGCCTGCCAC AAGCCAGGGC
    ACTGCAAGCA AATGCCCTTT CCTGGCAGCA CAGATGAATC AGAGAGGCAG CAGTGTCTTC
    TGCAAAGCCA GTCTTGAGCT TCAGGAGGAT GTGCAGGAAA TGAATGCCGT GAGGAAAGAG
    GTTGCTGAAA CCTCAGCAGG CCCCAGTGTG GTTAGTGTGA AAACCGATGG AGGGGATCCC
    AGTGGACTGC TGAAGAACTT CCAGGACATC ATGCAAAAGC AAAGACCAGA AAGAGTGTCT
    CATCTTCTTC AAGATAACTT GCCAAAATCT GTTTCCACTT TTCAGTATGA TCGTTTCTTT
    GAGAAAAAAA TTGATGAGAA AAAGAATGAC CACACCTATC GAGTTTTTAA AACTGTGAAC
    CGGCGAGCAC ACATCTTCCC CATGGCAGAT GACTATTCAG ACTCCCTCAT CACCAAAAAG
    CAAGTGTCAG TCTGGTGCAG TAATGACTAC CTAGGAATGA GTCGCCACCC ACGGGTGTGT
    GGGGCAGTTA TGGACACTTT GAAACAACAT GGTGCTGGGG CAGGTGGTAC TAGAAATATT
    TCTGGAACTA GTAAATTCCA TGTGGACTTA GAGCGGGAGC TGGCAGACCT CCATGGGAAA
    GATGCCGCAC TCTTGTTTTC CTCGTGCTTT GTGGCCAATG ACTCAACCCT CTTCACCCTG
    GCTAAGATGA TGCCAGGCTG TGAGATTTAC TCTGATTCTG GGAACCATGC CTCCATGATC
    CAAGGGATTC GAAACAGCCG AGTGCCAAAG TACATCTTCC GCCACAATGA TGTCAGCCAC
    CTCAGAGAAC TGCTGCAAAG ATCTGACCCC TCAGTCCCCA AGATTGTGGC ATTTGAAACT
    GTCCATTCAA TGGATGGCAA AGCCTTTGGT TGTGTTGGAG GGTACATCGC CAGCACGAGT
    TCTCTGATTG ACACCGTACG GTCCTATGCT GCTGGCTTCA TCTTCACCAC CTCTCTGCCA
    CCCATGCTGC TGGCTGGAGC CCTGGAGTCT GTGCGGATCC TGAAGAGCGC TGAGGGACGG
    GTGCTTCGCC GCCAGCACCA GCGCAACGTC AAACTCATGA GACAGATGCT AATGGATGCC
    GGCCTCCCTG TTGTCCACTG CCCCAGCCAC ATCATCCCTG TGCGGGTTGC AGATGCTGCT
    AAAAACACAG AAGTCTGTGA TGAACTAATG AGCAGACATA ACATCTACGT GCAAGCAATC
    AATTACCCTA CGGTGCCCCG GGGAGAAGAG CTCCTACGGA TTGCCCCCAC CCCTCACCAC
    ACACCCCAGA TGATGAACTA CTTCCTTGAG AATCTGCTAG TCACATGGAA GCAAGTGGGG
    CTGGAACTGA AGCCTCATTC CTCAGCTGAG TGCAACTTCT GCAGGAGGCC ACTGCATTTT
    GAAGTGATGA GTGAAAGAGA GAAGTCCTAT TTCTCAGGCT TGAGCAAGTT GGTATCTGCT
    CAGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu93882
    Clone ID Related Accession (Same CDS sequence) XM_017005873.1 , XM_024453393.1
    Accession Version XM_017005873.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1758bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product 5-aminolevulinate synthase, nonspecific, mitochondrial isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGAGAGTG TTGTTCGCCG CTGCCCATTC TTATCCCGAG TCCCCCAGGC CTTTCTGCAG 
    AAAGCAGGCA AATCTCTGTT GTTCTATGCC CAAAACTGCC CCAAGATGAT GGAAGTTGGG
    GCCAAGCCAG CCCCTCGGGC ATTGTCCACT GCAGCAGTAC ACTACCAACA GATCAAAGAA
    ACCCCTCCGG CCAGTGAGAA AGACAAAACT GCTAAGGCCA AGGTCCAACA GACTCCTGAT
    GGATCCCAGC AGAGTCCAGA TGGCACACAG CTTCCGTCTG GACACCCCTT GCCTGCCACA
    AGCCAGGGCA CTGCAAGCAA ATGCCCTTTC CTGGCAGCAC AGATGAATCA GAGAGGCAGC
    AGTGTCTTCT GCAAAGCCAG TCTTGAGCTT CAGGAGGATG TGCAGGAAAT GAATGCCGTG
    AGGAAAGAGG TTGCTGAAAC CTCAGCAGGC CCCAGTGTGG TTAGTGTGAA AACCGATGGA
    GGGGATCCCA GTGGACTGCT GAAGAACTTC CAGGACATCA TGCAAAAGCA AAGACCAGAA
    AGAGTGTCTC ATCTTCTTCA AGATAACTTG CCAAAATCTG TTTCCACTTT TCAGTATGAT
    CGTTTCTTTG AGAAAAAAAT TGATGAGAAA AAGAATGACC ACACCTATCG AGTTTTTAAA
    ACTGTGAACC GGCGAGCACA CATCTTCCCC ATGGCAGATG ACTATTCAGA CTCCCTCATC
    ACCAAAAAGC AAGTGTCAGT CTGGTGCAGT AATGACTACC TAGGAATGAG TCGCCACCCA
    CGGGTGTGTG GGGCAGTTAT GGACACTTTG AAACAACATG GTGCTGGGGC AGGTGGTACT
    AGAAATATTT CTGGAACTAG TAAATTCCAT GTGGACTTAG AGCGGGAGCT GGCAGACCTC
    CATGGGAAAG ATGCCGCACT CTTGTTTTCC TCGTGCTTTG TGGCCAATGA CTCAACCCTC
    TTCACCCTGG CTAAGATGAT GCCAGGCTGT GAGATTTACT CTGATTCTGG GAACCATGCC
    TCCATGATCC AAGGGATTCG AAACAGCCGA GTGCCAAAGT ACATCTTCCG CCACAATGAT
    GTCAGCCACC TCAGAGAACT GCTGCAAAGA TCTGACCCCT CAGTCCCCAA GATTGTGGCA
    TTTGAAACTG TCCATTCAAT GGATGGCAAA GCCTTTGGTT GTGTTGGAGG GTACATCGCC
    AGCACGAGTT CTCTGATTGA CACCGTACGG TCCTATGCTG CTGGCTTCAT CTTCACCACC
    TCTCTGCCAC CCATGCTGCT GGCTGGAGCC CTGGAGTCTG TGCGGATCCT GAAGAGCGCT
    GAGGGACGGG TGCTTCGCCG CCAGCACCAG CGCAACGTCA AACTCATGAG ACAGATGCTA
    ATGGATGCCG GCCTCCCTGT TGTCCACTGC CCCAGCCACA TCATCCCTGT GCGGGTTGCA
    GATGCTGCTA AAAACACAGA AGTCTGTGAT GAACTAATGA GCAGACATAA CATCTACGTG
    CAAGCAATCA ATTACCCTAC GGTGCCCCGG GGAGAAGAGC TCCTACGGAT TGCCCCCACC
    CCTCACCACA CACCCCAGAT GATGAACTAC TTCCTTGAGA ATCTGCTAGT CACATGGAAG
    CAAGTGGGGC TGGAACTGAA GCCTCATTCC TCAGCTGAGT GCAACTTCTG CAGGAGGCCA
    CTGCATTTTG AAGTGATGAG TGAAAGAGAG AAGTCCTATT TCTCAGGCTT GAGCAAGTTG
    GTATCTGCTC AGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861362.1
    CDS183..1940
    Translation

    Target ORF information:

    RefSeq Version XM_017005873.1
    Organism Homo sapiens(human)
    Definition Homo sapiens 5'-aminolevulinate synthase 1 (ALAS1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005873.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGAGAGTG TTGTTCGCCG CTGCCCATTC TTATCCCGAG TCCCCCAGGC CTTTCTGCAG 
    AAAGCAGGCA AATCTCTGTT GTTCTATGCC CAAAACTGCC CCAAGATGAT GGAAGTTGGG
    GCCAAGCCAG CCCCTCGGGC ATTGTCCACT GCAGCAGTAC ACTACCAACA GATCAAAGAA
    ACCCCTCCGG CCAGTGAGAA AGACAAAACT GCTAAGGCCA AGGTCCAACA GACTCCTGAT
    GGATCCCAGC AGAGTCCAGA TGGCACACAG CTTCCGTCTG GACACCCCTT GCCTGCCACA
    AGCCAGGGCA CTGCAAGCAA ATGCCCTTTC CTGGCAGCAC AGATGAATCA GAGAGGCAGC
    AGTGTCTTCT GCAAAGCCAG TCTTGAGCTT CAGGAGGATG TGCAGGAAAT GAATGCCGTG
    AGGAAAGAGG TTGCTGAAAC CTCAGCAGGC CCCAGTGTGG TTAGTGTGAA AACCGATGGA
    GGGGATCCCA GTGGACTGCT GAAGAACTTC CAGGACATCA TGCAAAAGCA AAGACCAGAA
    AGAGTGTCTC ATCTTCTTCA AGATAACTTG CCAAAATCTG TTTCCACTTT TCAGTATGAT
    CGTTTCTTTG AGAAAAAAAT TGATGAGAAA AAGAATGACC ACACCTATCG AGTTTTTAAA
    ACTGTGAACC GGCGAGCACA CATCTTCCCC ATGGCAGATG ACTATTCAGA CTCCCTCATC
    ACCAAAAAGC AAGTGTCAGT CTGGTGCAGT AATGACTACC TAGGAATGAG TCGCCACCCA
    CGGGTGTGTG GGGCAGTTAT GGACACTTTG AAACAACATG GTGCTGGGGC AGGTGGTACT
    AGAAATATTT CTGGAACTAG TAAATTCCAT GTGGACTTAG AGCGGGAGCT GGCAGACCTC
    CATGGGAAAG ATGCCGCACT CTTGTTTTCC TCGTGCTTTG TGGCCAATGA CTCAACCCTC
    TTCACCCTGG CTAAGATGAT GCCAGGCTGT GAGATTTACT CTGATTCTGG GAACCATGCC
    TCCATGATCC AAGGGATTCG AAACAGCCGA GTGCCAAAGT ACATCTTCCG CCACAATGAT
    GTCAGCCACC TCAGAGAACT GCTGCAAAGA TCTGACCCCT CAGTCCCCAA GATTGTGGCA
    TTTGAAACTG TCCATTCAAT GGATGGCAAA GCCTTTGGTT GTGTTGGAGG GTACATCGCC
    AGCACGAGTT CTCTGATTGA CACCGTACGG TCCTATGCTG CTGGCTTCAT CTTCACCACC
    TCTCTGCCAC CCATGCTGCT GGCTGGAGCC CTGGAGTCTG TGCGGATCCT GAAGAGCGCT
    GAGGGACGGG TGCTTCGCCG CCAGCACCAG CGCAACGTCA AACTCATGAG ACAGATGCTA
    ATGGATGCCG GCCTCCCTGT TGTCCACTGC CCCAGCCACA TCATCCCTGT GCGGGTTGCA
    GATGCTGCTA AAAACACAGA AGTCTGTGAT GAACTAATGA GCAGACATAA CATCTACGTG
    CAAGCAATCA ATTACCCTAC GGTGCCCCGG GGAGAAGAGC TCCTACGGAT TGCCCCCACC
    CCTCACCACA CACCCCAGAT GATGAACTAC TTCCTTGAGA ATCTGCTAGT CACATGGAAG
    CAAGTGGGGC TGGAACTGAA GCCTCATTCC TCAGCTGAGT GCAACTTCTG CAGGAGGCCA
    CTGCATTTTG AAGTGATGAG TGAAAGAGAG AAGTCCTATT TCTCAGGCTT GAGCAAGTTG
    GTATCTGCTC AGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu93882
    Clone ID Related Accession (Same CDS sequence) XM_017005873.1 , XM_024453393.1
    Accession Version XM_024453393.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1758bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product 5-aminolevulinate synthase, nonspecific, mitochondrial isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGAGAGTG TTGTTCGCCG CTGCCCATTC TTATCCCGAG TCCCCCAGGC CTTTCTGCAG 
    AAAGCAGGCA AATCTCTGTT GTTCTATGCC CAAAACTGCC CCAAGATGAT GGAAGTTGGG
    GCCAAGCCAG CCCCTCGGGC ATTGTCCACT GCAGCAGTAC ACTACCAACA GATCAAAGAA
    ACCCCTCCGG CCAGTGAGAA AGACAAAACT GCTAAGGCCA AGGTCCAACA GACTCCTGAT
    GGATCCCAGC AGAGTCCAGA TGGCACACAG CTTCCGTCTG GACACCCCTT GCCTGCCACA
    AGCCAGGGCA CTGCAAGCAA ATGCCCTTTC CTGGCAGCAC AGATGAATCA GAGAGGCAGC
    AGTGTCTTCT GCAAAGCCAG TCTTGAGCTT CAGGAGGATG TGCAGGAAAT GAATGCCGTG
    AGGAAAGAGG TTGCTGAAAC CTCAGCAGGC CCCAGTGTGG TTAGTGTGAA AACCGATGGA
    GGGGATCCCA GTGGACTGCT GAAGAACTTC CAGGACATCA TGCAAAAGCA AAGACCAGAA
    AGAGTGTCTC ATCTTCTTCA AGATAACTTG CCAAAATCTG TTTCCACTTT TCAGTATGAT
    CGTTTCTTTG AGAAAAAAAT TGATGAGAAA AAGAATGACC ACACCTATCG AGTTTTTAAA
    ACTGTGAACC GGCGAGCACA CATCTTCCCC ATGGCAGATG ACTATTCAGA CTCCCTCATC
    ACCAAAAAGC AAGTGTCAGT CTGGTGCAGT AATGACTACC TAGGAATGAG TCGCCACCCA
    CGGGTGTGTG GGGCAGTTAT GGACACTTTG AAACAACATG GTGCTGGGGC AGGTGGTACT
    AGAAATATTT CTGGAACTAG TAAATTCCAT GTGGACTTAG AGCGGGAGCT GGCAGACCTC
    CATGGGAAAG ATGCCGCACT CTTGTTTTCC TCGTGCTTTG TGGCCAATGA CTCAACCCTC
    TTCACCCTGG CTAAGATGAT GCCAGGCTGT GAGATTTACT CTGATTCTGG GAACCATGCC
    TCCATGATCC AAGGGATTCG AAACAGCCGA GTGCCAAAGT ACATCTTCCG CCACAATGAT
    GTCAGCCACC TCAGAGAACT GCTGCAAAGA TCTGACCCCT CAGTCCCCAA GATTGTGGCA
    TTTGAAACTG TCCATTCAAT GGATGGCAAA GCCTTTGGTT GTGTTGGAGG GTACATCGCC
    AGCACGAGTT CTCTGATTGA CACCGTACGG TCCTATGCTG CTGGCTTCAT CTTCACCACC
    TCTCTGCCAC CCATGCTGCT GGCTGGAGCC CTGGAGTCTG TGCGGATCCT GAAGAGCGCT
    GAGGGACGGG TGCTTCGCCG CCAGCACCAG CGCAACGTCA AACTCATGAG ACAGATGCTA
    ATGGATGCCG GCCTCCCTGT TGTCCACTGC CCCAGCCACA TCATCCCTGT GCGGGTTGCA
    GATGCTGCTA AAAACACAGA AGTCTGTGAT GAACTAATGA GCAGACATAA CATCTACGTG
    CAAGCAATCA ATTACCCTAC GGTGCCCCGG GGAGAAGAGC TCCTACGGAT TGCCCCCACC
    CCTCACCACA CACCCCAGAT GATGAACTAC TTCCTTGAGA ATCTGCTAGT CACATGGAAG
    CAAGTGGGGC TGGAACTGAA GCCTCATTCC TCAGCTGAGT GCAACTTCTG CAGGAGGCCA
    CTGCATTTTG AAGTGATGAG TGAAAGAGAG AAGTCCTATT TCTCAGGCTT GAGCAAGTTG
    GTATCTGCTC AGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024309161.1
    CDS360..2117
    Translation

    Target ORF information:

    RefSeq Version XM_024453393.1
    Organism Homo sapiens(human)
    Definition Homo sapiens 5'-aminolevulinate synthase 1 (ALAS1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024453393.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGAGAGTG TTGTTCGCCG CTGCCCATTC TTATCCCGAG TCCCCCAGGC CTTTCTGCAG 
    AAAGCAGGCA AATCTCTGTT GTTCTATGCC CAAAACTGCC CCAAGATGAT GGAAGTTGGG
    GCCAAGCCAG CCCCTCGGGC ATTGTCCACT GCAGCAGTAC ACTACCAACA GATCAAAGAA
    ACCCCTCCGG CCAGTGAGAA AGACAAAACT GCTAAGGCCA AGGTCCAACA GACTCCTGAT
    GGATCCCAGC AGAGTCCAGA TGGCACACAG CTTCCGTCTG GACACCCCTT GCCTGCCACA
    AGCCAGGGCA CTGCAAGCAA ATGCCCTTTC CTGGCAGCAC AGATGAATCA GAGAGGCAGC
    AGTGTCTTCT GCAAAGCCAG TCTTGAGCTT CAGGAGGATG TGCAGGAAAT GAATGCCGTG
    AGGAAAGAGG TTGCTGAAAC CTCAGCAGGC CCCAGTGTGG TTAGTGTGAA AACCGATGGA
    GGGGATCCCA GTGGACTGCT GAAGAACTTC CAGGACATCA TGCAAAAGCA AAGACCAGAA
    AGAGTGTCTC ATCTTCTTCA AGATAACTTG CCAAAATCTG TTTCCACTTT TCAGTATGAT
    CGTTTCTTTG AGAAAAAAAT TGATGAGAAA AAGAATGACC ACACCTATCG AGTTTTTAAA
    ACTGTGAACC GGCGAGCACA CATCTTCCCC ATGGCAGATG ACTATTCAGA CTCCCTCATC
    ACCAAAAAGC AAGTGTCAGT CTGGTGCAGT AATGACTACC TAGGAATGAG TCGCCACCCA
    CGGGTGTGTG GGGCAGTTAT GGACACTTTG AAACAACATG GTGCTGGGGC AGGTGGTACT
    AGAAATATTT CTGGAACTAG TAAATTCCAT GTGGACTTAG AGCGGGAGCT GGCAGACCTC
    CATGGGAAAG ATGCCGCACT CTTGTTTTCC TCGTGCTTTG TGGCCAATGA CTCAACCCTC
    TTCACCCTGG CTAAGATGAT GCCAGGCTGT GAGATTTACT CTGATTCTGG GAACCATGCC
    TCCATGATCC AAGGGATTCG AAACAGCCGA GTGCCAAAGT ACATCTTCCG CCACAATGAT
    GTCAGCCACC TCAGAGAACT GCTGCAAAGA TCTGACCCCT CAGTCCCCAA GATTGTGGCA
    TTTGAAACTG TCCATTCAAT GGATGGCAAA GCCTTTGGTT GTGTTGGAGG GTACATCGCC
    AGCACGAGTT CTCTGATTGA CACCGTACGG TCCTATGCTG CTGGCTTCAT CTTCACCACC
    TCTCTGCCAC CCATGCTGCT GGCTGGAGCC CTGGAGTCTG TGCGGATCCT GAAGAGCGCT
    GAGGGACGGG TGCTTCGCCG CCAGCACCAG CGCAACGTCA AACTCATGAG ACAGATGCTA
    ATGGATGCCG GCCTCCCTGT TGTCCACTGC CCCAGCCACA TCATCCCTGT GCGGGTTGCA
    GATGCTGCTA AAAACACAGA AGTCTGTGAT GAACTAATGA GCAGACATAA CATCTACGTG
    CAAGCAATCA ATTACCCTAC GGTGCCCCGG GGAGAAGAGC TCCTACGGAT TGCCCCCACC
    CCTCACCACA CACCCCAGAT GATGAACTAC TTCCTTGAGA ATCTGCTAGT CACATGGAAG
    CAAGTGGGGC TGGAACTGAA GCCTCATTCC TCAGCTGAGT GCAACTTCTG CAGGAGGCCA
    CTGCATTTTG AAGTGATGAG TGAAAGAGAG AAGTCCTATT TCTCAGGCTT GAGCAAGTTG
    GTATCTGCTC AGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.