F8 cDNA ORF clone, Homo sapiens(human)
Gene Symbol | F8 |
Entrez Gene ID | 2157 |
Full Name | coagulation factor VIII |
Synonyms | AHF,DXS1253E,F8B,F8C,FVIII,HEMA |
General protein information |
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Gene Type | protein-coding |
Organism | Homo sapiens(human) |
Genome | |
Summary | This gene encodes coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder. [provided by RefSeq, Jul 2008]. |
Disorder MIM: | |
Disorder Html: | Hemophilia A, 306700 (3) |
mRNA and Protein(s)
mRNA | Protein | Name |
---|---|---|
NM_000132.3 | NP_000123.1 | coagulation factor VIII isoform a preproprotein |
NM_019863.2 | NP_063916.1 | coagulation factor VIII isoform b |

Homo sapiens (human) | F8 | NP_000123.1 |
Canis lupus familiaris (dog) | F8 | NP_001003212.1 |
Pan troglodytes (chimpanzee) | F8 | XP_003317837.1 |
LOC100424151 | XP_002806506.1 | |
Rattus norvegicus (Norway rat) | F8 | NP_899160.1 |
Mus musculus (house mouse) | F8 | NP_032003.2 |
Bos taurus (cattle) | F8 | NP_001138980.1 |

The following F8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the F8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
CloneID | OHu23554![]() |
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Clone ID Related Accession (Same CDS sequence) | NM_019863.2 | |
Accession Version | NM_019863.2 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 651bp) Protein sequence SNP |
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Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2020-01-01 | |
Organism | Homo sapiens(human) | |
Product | coagulation factor VIII isoform b | |
Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI547023.1, BC064380.1 and BC022513.1. On May 19, 2005 this sequence version replaced NM_019863.1. Transcript Variant: This variant (2) contains an unique 5' exon located within intron 22 of transcript variant 1. This exon codes for eight amino acids and is spliced to exons 23-26 maintaining the reading frame. The resulting isoform (b) is considerably shorter compared to isoform a, and includes the phospholipid binding domain. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC098389.1, BC064380.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. |
1 | ATGCGGATCC AAGACCCTGG GAAGGTCTTC TTTGGCAATG TGGATTCATC TGGGATAAAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_063916.1 |
CDS | 146..796 |
Translation |

Target ORF information:
Target ORF information:
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ORF Insert Sequence:
1 | ATGCGGATCC AAGACCCTGG GAAGGTCTTC TTTGGCAATG TGGATTCATC TGGGATAAAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu23783![]() |
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Clone ID Related Accession (Same CDS sequence) | NM_000132.3 | |
Accession Version | NM_000132.3 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 7056bp) Protein sequence SNP |
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Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2020-01-01 | |
Organism | Homo sapiens(human) | |
Product | coagulation factor VIII isoform a preproprotein | |
Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from M14113.1 and BC022513.1. This sequence is a reference standard in the RefSeqGene project. On Jun 24, 2008 this sequence version replaced NM_000132.2. Transcript Variant: This variant (1) consists of 26 exons and encodes the full-length isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M14113.1, K01740.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. |
1 | ATGCAAATAG AGCTCTCCAC CTGCTTCTTT CTGTGCCTTT TGCGATTCTG CTTTAGTGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_000123.1 |
CDS | 172..7227 |
Translation |

Target ORF information:
Target ORF information:
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ORF Insert Sequence:
1 | ATGCAAATAG AGCTCTCCAC CTGCTTCTTT CTGTGCCTTT TGCGATTCTG CTTTAGTGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.