Nags cDNA ORF clone, Mus musculus(house mouse)
| Gene Symbol | Nags |
| Entrez Gene ID | 217214 |
| Full Name | N-acetylglutamate synthase |
| Synonyms | 1700120E20Rik,argA |
| General protein information |
|
| Gene Type | protein-coding |
| Organism | Mus musculus(house mouse) |
| Genome |
mRNA and Protein(s)
| mRNA | Protein | Name |
|---|---|---|
| NM_145829.2 | NP_665828.1 | N-acetylglutamate synthase, mitochondrial precursor |
| NAGS | XP_001114030.1 | |
| Eremothecium gossypii | AGOS_ADL062W | NP_984034.2 |
| Xenopus tropicalis (tropical clawed frog) | nags | XP_002935592.1 |
| Canis lupus familiaris (dog) | NAGS | XP_548066.2 |
| Rattus norvegicus (Norway rat) | Nags | NP_001100523.1 |
| Pan troglodytes (chimpanzee) | NAGS | XP_001152548.1 |
| Bos taurus (cattle) | NAGS | XP_002696085.1 |
| Mus musculus (house mouse) | Nags | NP_665828.1 |
| Danio rerio (zebrafish) | LOC557716 | XP_685919.3 |
| Homo sapiens (human) | NAGS | NP_694551.1 |
Related articles in PubMed
A novel UPLC-MS/MS based method to determine the activity of N-acetylglutamate synthase in liver tissue.
Dercksen M, Duran M, IJlst L, Kulik W, Ruiter JP, van Cruchten A, Tuchman M, Wanders RJ
Molecular genetics and metabolism119(4)307-310(2016 12)
A novel biochemically salvageable animal model of hyperammonemia devoid of N-acetylglutamate synthase.
Senkevitch E, Cabrera-Luque J, Morizono H, Caldovic L, Tuchman M
Molecular genetics and metabolism106(2)160-8(2012 Jun)
Transcriptional regulation of N-acetylglutamate synthase.
Heibel SK, Lopez GY, Panglao M, Sodha S, Mari?o-Ram?rez L, Tuchman M, Caldovic L
PloS one7(2)e29527(2012)
Down-regulation of hepatic urea synthesis by oxypurines: xanthine and uric acid inhibit N-acetylglutamate synthase.
Nissim I, Horyn O, Nissim I, Daikhin Y, Caldovic L, Barcelona B, Cervera J, Tuchman M, Yudkoff M
The Journal of biological chemistry286(25)22055-68(2011 Jun)
N-acetylglutamate synthase: structure, function and defects.
Caldovic L, Ah Mew N, Shi D, Morizono H, Yudkoff M, Tuchman M
Molecular genetics and metabolism100 Suppl 1S13-9(2010)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
The specificity of the assay was validated by demonstrating a complete deficiency of NAGS in liver homogenates from Nags -/- mice. CONCLUSION: The novel NAGS enzyme assay reported herein can be used for the diagnosis of inherited NAGS deficiency and may also be of value in the study of secondary hyperammonemia present in various inborn errors of metabolism as well as drug treatment.
Title: A novel UPLC-MS/MS based method to determine the activity of N-acetylglutamate synthase in liver tissue.
Sp1, CREB, HNF-1, and NF-Y, known to be responsive to hormones and diet, regulate NAGS transcription
Title: Transcriptional regulation of N-acetylglutamate synthase.
The biochemical properties of purified recombinant human and mouse NAGS-M and NAGS-C were determined in this study with the goal of better understanding the role of the variable domain in NAGS function.
Title: Biochemical properties of recombinant human and mouse N-acetylglutamate synthase.
Is a mitochondrial enzyme that catalyzes the formation of N-acetylglutamate (NAG)
Title: Mammalian N-acetylglutamate synthase.
identification, cloning and expression
Title: Identification, cloning and expression of the mouse N-acetylglutamate synthase gene.
The following Nags gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nags cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OMu11974 | |
| Clone ID Related Accession (Same CDS sequence) | NM_145829.2 | |
| Accession Version | NM_145829.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1584bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-02-22 | |
| Organism | Mus musculus(house mouse) | |
| Product | N-acetylglutamate synthase, mitochondrial precursor | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL591145.25, AK075765.1 and AI415708.1. On Oct 6, 2012 this sequence version replaced NM_145829.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC057990.1, AF462069.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 15050968; reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGCGACGG CGTGGGTGGC CACAGCCCTG CGGTCTGCTG CAGCGGCCAG AAGGTTGCGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_665828.1 |
| CDS | 75..1658 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_145829.2 |
ORF Insert Sequence:
1 | ATGGCGACGG CGTGGGTGGC CACAGCCCTG CGGTCTGCTG CAGCGGCCAG AAGGTTGCGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
 |
A novel UPLC-MS/MS based method to determine the activity of N-acetylglutamate synthase in liver tissue. |
|
A novel biochemically salvageable animal model of hyperammonemia devoid of N-acetylglutamate synthase. |
|
Transcriptional regulation of N-acetylglutamate synthase. |
|
Down-regulation of hepatic urea synthesis by oxypurines: xanthine and uric acid inhibit N-acetylglutamate synthase. |
|
N-acetylglutamate synthase: structure, function and defects. |