Ambra1 cDNA ORF clone, Mus musculus(house mouse)

  • Gene

  • Clones

  • gRNAs

The following Ambra1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ambra1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu30300 XM_006499252.2
Latest version!
Mus musculus autophagy/beclin 1 regulator 1 (Ambra1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OMu30301 XM_006499253.2
Latest version!
Mus musculus autophagy/beclin 1 regulator 1 (Ambra1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OMu18657 NM_172669.3
Latest version!
Mus musculus autophagy/beclin 1 regulator 1 (Ambra1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OMu18871 NM_001080754.1
Latest version!
Mus musculus autophagy/beclin 1 regulator 1 (Ambra1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
View Old Accession Versions >>
OMu30301 XM_006499253.1 Mus musculus autophagy/beclin 1 regulator 1 (Ambra1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OMu30300 XM_006499252.1 Mus musculus autophagy/beclin 1 regulator 1 (Ambra1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu30300
    Clone ID Related Accession (Same CDS sequence) XM_006499252.1 , XM_006499252.2
    Accession Version XM_006499252.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3816bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006499252.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAAAGTTG TCCCAGAGAA GAATGCTGTA CGAATCCTCT GGGGCCGAGA ACGGGGCACT 
    CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCT GATAGTCCAC GCTCGACCTT CTTACTGGCC
    TTCAGCCCAG ACAGGACTCT CTTGGCATCC ACACATGTGA ACCATAACAT CTATATTACA
    GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATCGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ACCCCACCAT CTCAGGCCTT ATTGCTTCTG GTTGCCTAGA TGGGGAAGTT
    AGGATTTGGG ATTTACATGG GGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATA
    GCCTCCTTGG CTTTCCACCC TACGGCTCAG CTTCTACTAA TTGCCACTGC AAATGAGATC
    CATTTCTGGG ATTGGAGTCG TCGGGAGCCC TTTGCTGTGG TGAAAACAGC CAGTGAGATG
    GAGCGTGTCC GCCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTTAC AGCTATTGTT
    AACCCTTCTA ATCAGCAGGG TGATGACGAA CCAGAAATCC CTATAGATGG AACAGAGTTA
    TCTCATTACC GTCAACGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGT TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC
    ACAGTACAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG
    CGTCCCAGGA CTTCCGCTTA CATCAGACTC CGACAGCGGG TCAGTTACCC CACCACAGTT
    GAGTGCTGCC AGCACCCTGG GATCCTGTGC CTTTGCAGCC GCTGTGCTGG CACTCGAGTT
    CCTTCCCTCT TGCCACACCA GGACAGTGTC CCCCCTGCTT CTGCCAGAGC TACTACCCCT
    TCCTTTTCTT TTGTACAGAC CGAGCCCTTC CATCCCCCGG AGCAGGCTTC ATCAACGCAG
    CAGGACCAGG GCCTCCTGAA CCGGCCGTCT GCCTTCAGTA CAGTCCAGAG CAGCACTGCG
    GGCAACACGC TCCGCAACCT CAGTCTGGGT CCTACCCGGC GCTCTTTGGG GGGCCCTTTG
    TCTAGCCACC CTTCTAGGTA TCACAGAGAA CTAGCCCCTG GGCTGACAGG TTCTGAGTGG
    ACCCGGACAG TACTCACTCT GAACTCTCGT TCTGAGGTAG AATCTATGCC CCCACCGAGG
    ACCAGTGCCT CCTCAGTGAG TTTGCTTTCT GTACTGAGAC AGCAGGAAGG TGGCTCTCAG
    GCATCTGTGT ATACTTCAGC CACAGAAGGG AGGGGTTTTC CATCATCAGG GTTGGCAACT
    GAGTCAGATG GAGGGAATGG TTCCAGCCAA AACAACTCAG GCAGCATTCG CCATGAGCTT
    CAATGTGACC TGAGACGCTT CTTTTTGGAG TATGACAGGC TGCAGGAGCT GGATCAGAGC
    CTGAGTGGGG AAACTCCCCA GACCCAACAG GCCCAGGAAA TGCTCAACAA TAATATTGAA
    TCTGAGAGAC CAGGTCCTTC CCACCTGCCT ACCCCACACA GCAGTGAGAA CAACTCCAAC
    CTGTCTCGGG GCCACCTTAA TCGATGCCGT GCTTGCCATA ATCTTCTAAC CTTCAACAAT
    GATACCCTTC GTTGGGAAAG AACCACACCT AACTACTCAT CTGGTGAGGC TAGCTCTTCC
    TGGCACGTCT CCACTACCTT TGAGGGCATG CCACCAAGTG GCAACCAGCT ACCACCTCTT
    GAGCGGACTG AGGGCCAAAT GCCTAGCTCC AGCAGGCTGG AGTTGAGCAG CTCTGCTAGT
    TCACAAGAGG AGAGGACTGT GGGGGTGGCC TTTAACCAGG AGACAGGCCA CTGGGAACGC
    ATTTATACCC AGTCCAGCAG ATCTGGAACT GTATCACAGG AGGCCTTACA TCAAGATATG
    CCTGAAGAAA GCTCTGAGGA AGACTCCCTC AGGAGATTAT CTCCTGCTGC ATACTACGCC
    CAGAGGATGA TCCAGTATCT CTCAAGGAGA GACAGTATTC GCCAGCGCTC CATGCGCTAC
    CAACAGAATC GGCTTCGTTC TTCCACCTCC TCTTCTTCCT CAGACAACCA GGGTCCATCA
    GTAGAGGGAA CCGACTTGGA ATTTGAGGAC TTTGAGGACA ATGGTGACAG ATCTAGGCAC
    CGAGCTCCCC GAAATGCCCG AATGTCTGCA CCTTCACTTG GACGATTTGT TCCAAGGCGT
    TTTTTGCTGC CTGAGTACTT GCCTTATGCT GGGATTTTTC ATGAACGTGG ACAGCCTGGC
    TTGGCTACTC ATTCTTCTGT TAACAGGGTC CTGGCAGGGG CAGTAATTGG AGATGGACAG
    TCTGCTGTGG CCAGTAACAT TGCCAATACT ACCTACAGGC TCCAGTGGTG GGACTTCACT
    AAGTTTGACC TTCCAGAAAT CAGTAATGCC TCTGTGAATG TCCTGGTACA GAATTGCAAG
    ATCTACAATG ATGCCAGCTG TGACATTTCT GCAGATGGTC AGCTCCTGGC AGCCTTCATC
    CCGAGCAGCC AGAGGGGCTT TCCTGATGAA GGCATCCTGG CAGTGTATTC CCTGGCCCCC
    CATAACTTGG GGGAAATGCT CTACACCAAG CGATTTGGTC CCAATGCCAT TTCTGTAAGC
    CTGTCCCCGA TGGGCCGATA CGTTATGGTG GGCTTGGCCT CACGAAGGAT CCTACTGCAC
    CCCTCCACAG AGCACATGGT AGCCCAGGTC TTCAGGCTGC AACAGGCCCA TGGTGGTGAG
    ACTTCCATGA GGAGAGTTTT CAATGTCCTT TACCCCATGC CTGCAGACCA ACGGAGACAC
    GTCAGCATCA ACTCTGCCCG TTGGCTACCC GAGCCAGGGC TTGGTTTGGC CTATGGCACC
    AACAAAGGAG ACTTGGTGAT CTGCCGACCA GAGGCCTTAA ACTCTGGCAT CGAGTACTAC
    TGGGACCAGC TAAGTGAAAC AGTCTTCACT GTCCACTCAA GTAGCAGGAG CAGCGAGCGG
    CCTGGAACCA GCAGAGCCAC GTGGCGGACA GACAGAGACA TGGGTTTGAT GAATGCAATT
    GGGCTGCAGC CTCGGAACCC AACCACCTCT GTGACATCTC AGGGCACCCA GACTCTGGCC
    CTCCAGTTGC AGAACGCTGA AACACAGACT GAGAGGGAGG AAGAGGAACC AGGGGCAGCA
    TCTTCAGGTC CTGGTGAAGG CGAGGGCTCA GAATATGGTG GCAGTGGGGA AGATGCCCTC
    AGCAGGATCC AGAGGCTGAT GGCCGAGGGT GGCATGACAG CTGTGGTACA ACGGGAACAG
    AGCACCACCA TGGCCTCCAT GGGTGGCTTT GGCAACAACA TCATCGTTAG CCACCGCATT
    CACCGCAGCT CCCAGACAGG CACAGAGTCA GGGGCTGCTC GTACCTCCTC ACCCCAGCCC
    TCCACCTCTC GAGGACTGCC ATCAGAACCT GGGCAACTGG CAGAGCGAGC GCTAAGCCCC
    CGGACAGCCT CCTGGGACCA GCCTAGTACC TCTGGGAGGG AACTACCCCA GCCAGCCCTG
    TCCTCTTCCT CCCCTGTCCC CATTCCAGTC CCCCTTGCCA GCAATGAGGG ACCAACCATG
    CACTGCAATG TGACCAATAA CAGTCACCTT CCTGAGGGTG ATGGTAGCAA CAGGGGGGAA
    GCTGCAGGCC CTAGTGGAGA GCCACAGAAC CGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006499315.1
    CDS342..4157
    Translation

    Target ORF information:

    RefSeq Version XM_006499252.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus autophagy/beclin 1 regulator 1 (Ambra1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006499252.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAAAGTTG TCCCAGAGAA GAATGCTGTA CGAATCCTCT GGGGCCGAGA ACGGGGCACT 
    CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCT GATAGTCCAC GCTCGACCTT CTTACTGGCC
    TTCAGCCCAG ACAGGACTCT CTTGGCATCC ACACATGTGA ACCATAACAT CTATATTACA
    GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATCGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ACCCCACCAT CTCAGGCCTT ATTGCTTCTG GTTGCCTAGA TGGGGAAGTT
    AGGATTTGGG ATTTACATGG GGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATA
    GCCTCCTTGG CTTTCCACCC TACGGCTCAG CTTCTACTAA TTGCCACTGC AAATGAGATC
    CATTTCTGGG ATTGGAGTCG TCGGGAGCCC TTTGCTGTGG TGAAAACAGC CAGTGAGATG
    GAGCGTGTCC GCCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTTAC AGCTATTGTT
    AACCCTTCTA ATCAGCAGGG TGATGACGAA CCAGAAATCC CTATAGATGG AACAGAGTTA
    TCTCATTACC GTCAACGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGT TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC
    ACAGTACAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG
    CGTCCCAGGA CTTCCGCTTA CATCAGACTC CGACAGCGGG TCAGTTACCC CACCACAGTT
    GAGTGCTGCC AGCACCCTGG GATCCTGTGC CTTTGCAGCC GCTGTGCTGG CACTCGAGTT
    CCTTCCCTCT TGCCACACCA GGACAGTGTC CCCCCTGCTT CTGCCAGAGC TACTACCCCT
    TCCTTTTCTT TTGTACAGAC CGAGCCCTTC CATCCCCCGG AGCAGGCTTC ATCAACGCAG
    CAGGACCAGG GCCTCCTGAA CCGGCCGTCT GCCTTCAGTA CAGTCCAGAG CAGCACTGCG
    GGCAACACGC TCCGCAACCT CAGTCTGGGT CCTACCCGGC GCTCTTTGGG GGGCCCTTTG
    TCTAGCCACC CTTCTAGGTA TCACAGAGAA CTAGCCCCTG GGCTGACAGG TTCTGAGTGG
    ACCCGGACAG TACTCACTCT GAACTCTCGT TCTGAGGTAG AATCTATGCC CCCACCGAGG
    ACCAGTGCCT CCTCAGTGAG TTTGCTTTCT GTACTGAGAC AGCAGGAAGG TGGCTCTCAG
    GCATCTGTGT ATACTTCAGC CACAGAAGGG AGGGGTTTTC CATCATCAGG GTTGGCAACT
    GAGTCAGATG GAGGGAATGG TTCCAGCCAA AACAACTCAG GCAGCATTCG CCATGAGCTT
    CAATGTGACC TGAGACGCTT CTTTTTGGAG TATGACAGGC TGCAGGAGCT GGATCAGAGC
    CTGAGTGGGG AAACTCCCCA GACCCAACAG GCCCAGGAAA TGCTCAACAA TAATATTGAA
    TCTGAGAGAC CAGGTCCTTC CCACCTGCCT ACCCCACACA GCAGTGAGAA CAACTCCAAC
    CTGTCTCGGG GCCACCTTAA TCGATGCCGT GCTTGCCATA ATCTTCTAAC CTTCAACAAT
    GATACCCTTC GTTGGGAAAG AACCACACCT AACTACTCAT CTGGTGAGGC TAGCTCTTCC
    TGGCACGTCT CCACTACCTT TGAGGGCATG CCACCAAGTG GCAACCAGCT ACCACCTCTT
    GAGCGGACTG AGGGCCAAAT GCCTAGCTCC AGCAGGCTGG AGTTGAGCAG CTCTGCTAGT
    TCACAAGAGG AGAGGACTGT GGGGGTGGCC TTTAACCAGG AGACAGGCCA CTGGGAACGC
    ATTTATACCC AGTCCAGCAG ATCTGGAACT GTATCACAGG AGGCCTTACA TCAAGATATG
    CCTGAAGAAA GCTCTGAGGA AGACTCCCTC AGGAGATTAT CTCCTGCTGC ATACTACGCC
    CAGAGGATGA TCCAGTATCT CTCAAGGAGA GACAGTATTC GCCAGCGCTC CATGCGCTAC
    CAACAGAATC GGCTTCGTTC TTCCACCTCC TCTTCTTCCT CAGACAACCA GGGTCCATCA
    GTAGAGGGAA CCGACTTGGA ATTTGAGGAC TTTGAGGACA ATGGTGACAG ATCTAGGCAC
    CGAGCTCCCC GAAATGCCCG AATGTCTGCA CCTTCACTTG GACGATTTGT TCCAAGGCGT
    TTTTTGCTGC CTGAGTACTT GCCTTATGCT GGGATTTTTC ATGAACGTGG ACAGCCTGGC
    TTGGCTACTC ATTCTTCTGT TAACAGGGTC CTGGCAGGGG CAGTAATTGG AGATGGACAG
    TCTGCTGTGG CCAGTAACAT TGCCAATACT ACCTACAGGC TCCAGTGGTG GGACTTCACT
    AAGTTTGACC TTCCAGAAAT CAGTAATGCC TCTGTGAATG TCCTGGTACA GAATTGCAAG
    ATCTACAATG ATGCCAGCTG TGACATTTCT GCAGATGGTC AGCTCCTGGC AGCCTTCATC
    CCGAGCAGCC AGAGGGGCTT TCCTGATGAA GGCATCCTGG CAGTGTATTC CCTGGCCCCC
    CATAACTTGG GGGAAATGCT CTACACCAAG CGATTTGGTC CCAATGCCAT TTCTGTAAGC
    CTGTCCCCGA TGGGCCGATA CGTTATGGTG GGCTTGGCCT CACGAAGGAT CCTACTGCAC
    CCCTCCACAG AGCACATGGT AGCCCAGGTC TTCAGGCTGC AACAGGCCCA TGGTGGTGAG
    ACTTCCATGA GGAGAGTTTT CAATGTCCTT TACCCCATGC CTGCAGACCA ACGGAGACAC
    GTCAGCATCA ACTCTGCCCG TTGGCTACCC GAGCCAGGGC TTGGTTTGGC CTATGGCACC
    AACAAAGGAG ACTTGGTGAT CTGCCGACCA GAGGCCTTAA ACTCTGGCAT CGAGTACTAC
    TGGGACCAGC TAAGTGAAAC AGTCTTCACT GTCCACTCAA GTAGCAGGAG CAGCGAGCGG
    CCTGGAACCA GCAGAGCCAC GTGGCGGACA GACAGAGACA TGGGTTTGAT GAATGCAATT
    GGGCTGCAGC CTCGGAACCC AACCACCTCT GTGACATCTC AGGGCACCCA GACTCTGGCC
    CTCCAGTTGC AGAACGCTGA AACACAGACT GAGAGGGAGG AAGAGGAACC AGGGGCAGCA
    TCTTCAGGTC CTGGTGAAGG CGAGGGCTCA GAATATGGTG GCAGTGGGGA AGATGCCCTC
    AGCAGGATCC AGAGGCTGAT GGCCGAGGGT GGCATGACAG CTGTGGTACA ACGGGAACAG
    AGCACCACCA TGGCCTCCAT GGGTGGCTTT GGCAACAACA TCATCGTTAG CCACCGCATT
    CACCGCAGCT CCCAGACAGG CACAGAGTCA GGGGCTGCTC GTACCTCCTC ACCCCAGCCC
    TCCACCTCTC GAGGACTGCC ATCAGAACCT GGGCAACTGG CAGAGCGAGC GCTAAGCCCC
    CGGACAGCCT CCTGGGACCA GCCTAGTACC TCTGGGAGGG AACTACCCCA GCCAGCCCTG
    TCCTCTTCCT CCCCTGTCCC CATTCCAGTC CCCCTTGCCA GCAATGAGGG ACCAACCATG
    CACTGCAATG TGACCAATAA CAGTCACCTT CCTGAGGGTG ATGGTAGCAA CAGGGGGGAA
    GCTGCAGGCC CTAGTGGAGA GCCACAGAAC CGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu30301
    Clone ID Related Accession (Same CDS sequence) XM_006499253.2 , XM_006499253.1
    Accession Version XM_006499253.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3543bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006499253.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAAAGTTG TCCCAGAGAA GAATGCTGTA CGAATCCTCT GGGGCCGAGA ACGGGGCACT 
    CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCT GATAGTCCAC GCTCGACCTT CTTACTGGCC
    TTCAGCCCAG ACAGGACTCT CTTGGCATCC ACACATGTGA ACCATAACAT CTATATTACA
    GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATCGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ACCCCACCAT CTCAGGCCTT ATTGCTTCTG GTTGCCTAGA TGGGGAAGTT
    AGGATTTGGG ATTTACATGG GGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATA
    GCCTCCTTGG CTTTCCACCC TACGGCTCAG CTTCTACTAA TTGCCACTGC AAATGAGATC
    CATTTCTGGG ATTGGAGTCG TCGGGAGCCC TTTGCTGTGG TGAAAACAGC CAGTGAGATG
    GAGCGTGTCC GCCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTTAC AGCTATTGTT
    AACCCTTCTA ATCAGCAGGG TGATGACGAA CCAGAAATCC CTATAGATGG AACAGAGTTA
    TCTCATTACC GTCAACGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGT TCCTCTGGCA TCCAGACCGA GCCCTTCCAT
    CCCCCGGAGC AGGCTTCATC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC
    TTCAGTACAG TCCAGAGCAG CACTGCGGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT
    ACCCGGCGCT CTTTGGGGGG CCCTTTGTCT AGCCACCCTT CTAGGTATCA CAGAGAACTA
    GCCCCTGGGC TGACAGGTTC TGAGTGGACC CGGACAGTAC TCACTCTGAA CTCTCGTTCT
    GAGGTAGAAT CTATGCCCCC ACCGAGGACC AGTGCCTCCT CAGTGAGTTT GCTTTCTGTA
    CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTATA CTTCAGCCAC AGAAGGGAGG
    GGTTTTCCAT CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGTTC CAGCCAAAAC
    AACTCAGGCA GCATTCGCCA TGAGCTTCAA TGTGACCTGA GACGCTTCTT TTTGGAGTAT
    GACAGGCTGC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAA CTCCCCAGAC CCAACAGGCC
    CAGGAAATGC TCAACAATAA TATTGAATCT GAGAGACCAG GTCCTTCCCA CCTGCCTACC
    CCACACAGCA GTGAGAACAA CTCCAACCTG TCTCGGGGCC ACCTTAATCG ATGCCGTGCT
    TGCCATAATC TTCTAACCTT CAACAATGAT ACCCTTCGTT GGGAAAGAAC CACACCTAAC
    TACTCATCTG GTGAGGCTAG CTCTTCCTGG CACGTCTCCA CTACCTTTGA GGGCATGCCA
    CCAAGTGGCA ACCAGCTACC ACCTCTTGAG CGGACTGAGG GCCAAATGCC TAGCTCCAGC
    AGGCTGGAGT TGAGCAGCTC TGCTAGTTCA CAAGAGGAGA GGACTGTGGG GGTGGCCTTT
    AACCAGGAGA CAGGCCACTG GGAACGCATT TATACCCAGT CCAGCAGATC TGGAACTGTA
    TCACAGGAGG CCTTACATCA AGATATGCCT GAAGAAAGCT CTGAGGAAGA CTCCCTCAGG
    AGATTATCTC CTGCTGCATA CTACGCCCAG AGGATGATCC AGTATCTCTC AAGGAGAGAC
    AGTATTCGCC AGCGCTCCAT GCGCTACCAA CAGAATCGGC TTCGTTCTTC CACCTCCTCT
    TCTTCCTCAG ACAACCAGGG TCCATCAGTA GAGGGAACCG ACTTGGAATT TGAGGACTTT
    GAGGACAATG GTGACAGATC TAGGCACCGA GCTCCCCGAA ATGCCCGAAT GTCTGCACCT
    TCACTTGGAC GATTTGTTCC AAGGCGTTTT TTGCTGCCTG AGTACTTGCC TTATGCTGGG
    ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCATT CTTCTGTTAA CAGGGTCCTG
    GCAGGGGCAG TAATTGGAGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC
    TACAGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTTC CAGAAATCAG TAATGCCTCT
    GTGAATGTCC TGGTACAGAA TTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA
    GATGGTCAGC TCCTGGCAGC CTTCATCCCG AGCAGCCAGA GGGGCTTTCC TGATGAAGGC
    ATCCTGGCAG TGTATTCCCT GGCCCCCCAT AACTTGGGGG AAATGCTCTA CACCAAGCGA
    TTTGGTCCCA ATGCCATTTC TGTAAGCCTG TCCCCGATGG GCCGATACGT TATGGTGGGC
    TTGGCCTCAC GAAGGATCCT ACTGCACCCC TCCACAGAGC ACATGGTAGC CCAGGTCTTC
    AGGCTGCAAC AGGCCCATGG TGGTGAGACT TCCATGAGGA GAGTTTTCAA TGTCCTTTAC
    CCCATGCCTG CAGACCAACG GAGACACGTC AGCATCAACT CTGCCCGTTG GCTACCCGAG
    CCAGGGCTTG GTTTGGCCTA TGGCACCAAC AAAGGAGACT TGGTGATCTG CCGACCAGAG
    GCCTTAAACT CTGGCATCGA GTACTACTGG GACCAGCTAA GTGAAACAGT CTTCACTGTC
    CACTCAAGTA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACGTG GCGGACAGAC
    AGAGACATGG GTTTGATGAA TGCAATTGGG CTGCAGCCTC GGAACCCAAC CACCTCTGTG
    ACATCTCAGG GCACCCAGAC TCTGGCCCTC CAGTTGCAGA ACGCTGAAAC ACAGACTGAG
    AGGGAGGAAG AGGAACCAGG GGCAGCATCT TCAGGTCCTG GTGAAGGCGA GGGCTCAGAA
    TATGGTGGCA GTGGGGAAGA TGCCCTCAGC AGGATCCAGA GGCTGATGGC CGAGGGTGGC
    ATGACAGCTG TGGTACAACG GGAACAGAGC ACCACCATGG CCTCCATGGG TGGCTTTGGC
    AACAACATCA TCGTTAGCCA CCGCATTCAC CGCAGCTCCC AGACAGGCAC AGAGTCAGGG
    GCTGCTCGTA CCTCCTCACC CCAGCCCTCC ACCTCTCGAG GACTGCCATC AGAACCTGGG
    CAACTGGCAG AGCGAGCGCT AAGCCCCCGG ACAGCCTCCT GGGACCAGCC TAGTACCTCT
    GGGAGGGAAC TACCCCAGCC AGCCCTGTCC TCTTCCTCCC CTGTCCCCAT TCCAGTCCCC
    CTTGCCAGCA ATGAGGGACC AACCATGCAC TGCAATGTGA CCAATAACAG TCACCTTCCT
    GAGGGTGATG GTAGCAACAG GGGGGAAGCT GCAGGCCCTA GTGGAGAGCC ACAGAACCGA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006499316.1
    CDS352..3894
    Translation

    Target ORF information:

    RefSeq Version XM_006499253.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus autophagy/beclin 1 regulator 1 (Ambra1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006499253.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAAAGTTG TCCCAGAGAA GAATGCTGTA CGAATCCTCT GGGGCCGAGA ACGGGGCACT 
    CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCT GATAGTCCAC GCTCGACCTT CTTACTGGCC
    TTCAGCCCAG ACAGGACTCT CTTGGCATCC ACACATGTGA ACCATAACAT CTATATTACA
    GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATCGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ACCCCACCAT CTCAGGCCTT ATTGCTTCTG GTTGCCTAGA TGGGGAAGTT
    AGGATTTGGG ATTTACATGG GGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATA
    GCCTCCTTGG CTTTCCACCC TACGGCTCAG CTTCTACTAA TTGCCACTGC AAATGAGATC
    CATTTCTGGG ATTGGAGTCG TCGGGAGCCC TTTGCTGTGG TGAAAACAGC CAGTGAGATG
    GAGCGTGTCC GCCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTTAC AGCTATTGTT
    AACCCTTCTA ATCAGCAGGG TGATGACGAA CCAGAAATCC CTATAGATGG AACAGAGTTA
    TCTCATTACC GTCAACGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGT TCCTCTGGCA TCCAGACCGA GCCCTTCCAT
    CCCCCGGAGC AGGCTTCATC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC
    TTCAGTACAG TCCAGAGCAG CACTGCGGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT
    ACCCGGCGCT CTTTGGGGGG CCCTTTGTCT AGCCACCCTT CTAGGTATCA CAGAGAACTA
    GCCCCTGGGC TGACAGGTTC TGAGTGGACC CGGACAGTAC TCACTCTGAA CTCTCGTTCT
    GAGGTAGAAT CTATGCCCCC ACCGAGGACC AGTGCCTCCT CAGTGAGTTT GCTTTCTGTA
    CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTATA CTTCAGCCAC AGAAGGGAGG
    GGTTTTCCAT CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGTTC CAGCCAAAAC
    AACTCAGGCA GCATTCGCCA TGAGCTTCAA TGTGACCTGA GACGCTTCTT TTTGGAGTAT
    GACAGGCTGC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAA CTCCCCAGAC CCAACAGGCC
    CAGGAAATGC TCAACAATAA TATTGAATCT GAGAGACCAG GTCCTTCCCA CCTGCCTACC
    CCACACAGCA GTGAGAACAA CTCCAACCTG TCTCGGGGCC ACCTTAATCG ATGCCGTGCT
    TGCCATAATC TTCTAACCTT CAACAATGAT ACCCTTCGTT GGGAAAGAAC CACACCTAAC
    TACTCATCTG GTGAGGCTAG CTCTTCCTGG CACGTCTCCA CTACCTTTGA GGGCATGCCA
    CCAAGTGGCA ACCAGCTACC ACCTCTTGAG CGGACTGAGG GCCAAATGCC TAGCTCCAGC
    AGGCTGGAGT TGAGCAGCTC TGCTAGTTCA CAAGAGGAGA GGACTGTGGG GGTGGCCTTT
    AACCAGGAGA CAGGCCACTG GGAACGCATT TATACCCAGT CCAGCAGATC TGGAACTGTA
    TCACAGGAGG CCTTACATCA AGATATGCCT GAAGAAAGCT CTGAGGAAGA CTCCCTCAGG
    AGATTATCTC CTGCTGCATA CTACGCCCAG AGGATGATCC AGTATCTCTC AAGGAGAGAC
    AGTATTCGCC AGCGCTCCAT GCGCTACCAA CAGAATCGGC TTCGTTCTTC CACCTCCTCT
    TCTTCCTCAG ACAACCAGGG TCCATCAGTA GAGGGAACCG ACTTGGAATT TGAGGACTTT
    GAGGACAATG GTGACAGATC TAGGCACCGA GCTCCCCGAA ATGCCCGAAT GTCTGCACCT
    TCACTTGGAC GATTTGTTCC AAGGCGTTTT TTGCTGCCTG AGTACTTGCC TTATGCTGGG
    ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCATT CTTCTGTTAA CAGGGTCCTG
    GCAGGGGCAG TAATTGGAGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC
    TACAGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTTC CAGAAATCAG TAATGCCTCT
    GTGAATGTCC TGGTACAGAA TTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA
    GATGGTCAGC TCCTGGCAGC CTTCATCCCG AGCAGCCAGA GGGGCTTTCC TGATGAAGGC
    ATCCTGGCAG TGTATTCCCT GGCCCCCCAT AACTTGGGGG AAATGCTCTA CACCAAGCGA
    TTTGGTCCCA ATGCCATTTC TGTAAGCCTG TCCCCGATGG GCCGATACGT TATGGTGGGC
    TTGGCCTCAC GAAGGATCCT ACTGCACCCC TCCACAGAGC ACATGGTAGC CCAGGTCTTC
    AGGCTGCAAC AGGCCCATGG TGGTGAGACT TCCATGAGGA GAGTTTTCAA TGTCCTTTAC
    CCCATGCCTG CAGACCAACG GAGACACGTC AGCATCAACT CTGCCCGTTG GCTACCCGAG
    CCAGGGCTTG GTTTGGCCTA TGGCACCAAC AAAGGAGACT TGGTGATCTG CCGACCAGAG
    GCCTTAAACT CTGGCATCGA GTACTACTGG GACCAGCTAA GTGAAACAGT CTTCACTGTC
    CACTCAAGTA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACGTG GCGGACAGAC
    AGAGACATGG GTTTGATGAA TGCAATTGGG CTGCAGCCTC GGAACCCAAC CACCTCTGTG
    ACATCTCAGG GCACCCAGAC TCTGGCCCTC CAGTTGCAGA ACGCTGAAAC ACAGACTGAG
    AGGGAGGAAG AGGAACCAGG GGCAGCATCT TCAGGTCCTG GTGAAGGCGA GGGCTCAGAA
    TATGGTGGCA GTGGGGAAGA TGCCCTCAGC AGGATCCAGA GGCTGATGGC CGAGGGTGGC
    ATGACAGCTG TGGTACAACG GGAACAGAGC ACCACCATGG CCTCCATGGG TGGCTTTGGC
    AACAACATCA TCGTTAGCCA CCGCATTCAC CGCAGCTCCC AGACAGGCAC AGAGTCAGGG
    GCTGCTCGTA CCTCCTCACC CCAGCCCTCC ACCTCTCGAG GACTGCCATC AGAACCTGGG
    CAACTGGCAG AGCGAGCGCT AAGCCCCCGG ACAGCCTCCT GGGACCAGCC TAGTACCTCT
    GGGAGGGAAC TACCCCAGCC AGCCCTGTCC TCTTCCTCCC CTGTCCCCAT TCCAGTCCCC
    CTTGCCAGCA ATGAGGGACC AACCATGCAC TGCAATGTGA CCAATAACAG TCACCTTCCT
    GAGGGTGATG GTAGCAACAG GGGGGAAGCT GCAGGCCCTA GTGGAGAGCC ACAGAACCGA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu18657
    Clone ID Related Accession (Same CDS sequence) NM_172669.3
    Accession Version NM_172669.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3903bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-25
    Organism Mus musculus(house mouse)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ150588.1, AK083596.1, AK129433.1 and BC032293.1. On Jan 24, 2007 this sequence version replaced NM_172669.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK155513.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAAGTTG TCCCAGAGAA GAATGCTGTA CGAATCCTCT GGGGCCGAGA ACGGGGCACT 
    CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCT GATAGTCCAC GCTCGACCTT CTTACTGGCC
    TTCAGCCCAG ACAGGACTCT CTTGGCATCC ACACATGTGA ACCATAACAT CTATATTACA
    GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATCGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ACCCCACCAT CTCAGGCCTT ATTGCTTCTG GTTGCCTAGA TGGGGAAGTT
    AGGATTTGGG ATTTACATGG GGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATA
    GCCTCCTTGG CTTTCCACCC TACGGCTCAG CTTCTACTAA TTGCCACTGC AAATGAGATC
    CATTTCTGGG ATTGGAGTCG TCGGGAGCCC TTTGCTGTGG TGAAAACAGC CAGTGAGATG
    GAGCGTGTCC GCCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTTAC AGCTATTGTT
    AACCCTTCTA ATCAGCAGGG TGATGACGAA CCAGAAATCC CTATAGATGG AACAGAGTTA
    TCTCATTACC GTCAACGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGT TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC
    ACAGTACAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG
    CGTCCCAGGA CTTCCGCTTA CATCAGACTC CGACAGCGGG TCAGTTACCC CACCACAGTT
    GAGTGCTGCC AGCACCCTGG GATCCTGTGC CTTTGCAGCC GCTGTGCTGG CACTCGAGTT
    CCTTCCCTCT TGCCACACCA GGACAGTGTC CCCCCTGCTT CTGCCAGAGC TACTACCCCT
    TCCTTTTCTT TTGTACAGAC CGAGCCCTTC CATCCCCCGG AGCAGGCTTC ATCAACGCAG
    CAGGACCAGG GCCTCCTGAA CCGGCCGTCT GCCTTCAGTA CAGTCCAGAG CAGCACTGCG
    GGCAACACGC TCCGCAACCT CAGTCTGGGT CCTACCCGGC GCTCTTTGGG GGGCCCTTTG
    TCTAGCCACC CTTCTAGGTA TCACAGAGAA CTAGCCCCTG GGCTGACAGG TTCTGAGTGG
    ACCCGGACAG TACTCACTCT GAACTCTCGT TCTGAGGTAG AATCTATGCC CCCACCGAGG
    ACCAGTGCCT CCTCAGTGAG TTTGCTTTCT GTACTGAGAC AGCAGGAAGG TGGCTCTCAG
    GCATCTGTGT ATACTTCAGC CACAGAAGGG AGGGGTTTTC CATCATCAGG GTTGGCAACT
    GAGTCAGATG GAGGGAATGG TTCCAGCCAA AACAACTCAG GCAGCATTCG CCATGAGCTT
    CAATGTGACC TGAGACGCTT CTTTTTGGAG TATGACAGGC TGCAGGAGCT GGATCAGAGC
    CTGAGTGGGG AAACTCCCCA GACCCAACAG GCCCAGGAAA TGCTCAACAA TAATATTGAA
    TCTGAGAGAC CAGGTCCTTC CCACCTGCCT ACCCCACACA GCAGTGAGAA CAACTCCAAC
    CTGTCTCGGG GCCACCTTAA TCGATGCCGT GCTTGCCATA ATCTTCTAAC CTTCAACAAT
    GATACCCTTC GTTGGGAAAG AACCACACCT AACTACTCAT CTGGTGAGGC TAGCTCTTCC
    TGGCACGTCT CCACTACCTT TGAGGGCATG CCACCAAGTG GCAACCAGCT ACCACCTCTT
    GAGCGGACTG AGGGCCAAAT GCCTAGCTCC AGCAGGCTGG AGTTGAGCAG CTCTGCTAGT
    TCACAAGAGG AGAGGACTGT GGGGGTGGCC TTTAACCAGG AGACAGGCCA CTGGGAACGC
    ATTTATACCC AGTCCAGCAG ATCTGGAACT GTATCACAGG AGGCCTTACA TCAAGATATG
    CCTGAAGAAA GCTCTGAGGA AGACTCCCTC AGGAGGAGGC TGCTGGAGTC TTCCCTCATT
    TCATTATCCC GTTATGATGG AGCAGGATCC AGAGAGCACC CAATTTACCC AGACCCAGCG
    AGATTATCTC CTGCTGCATA CTACGCCCAG AGGATGATCC AGTATCTCTC AAGGAGAGAC
    AGTATTCGCC AGCGCTCCAT GCGCTACCAA CAGAATCGGC TTCGTTCTTC CACCTCCTCT
    TCTTCCTCAG ACAACCAGGG TCCATCAGTA GAGGGAACCG ACTTGGAATT TGAGGACTTT
    GAGGACAATG GTGACAGATC TAGGCACCGA GCTCCCCGAA ATGCCCGAAT GTCTGCACCT
    TCACTTGGAC GATTTGTTCC AAGGCGTTTT TTGCTGCCTG AGTACTTGCC TTATGCTGGG
    ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCATT CTTCTGTTAA CAGGGTCCTG
    GCAGGGGCAG TAATTGGAGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC
    TACAGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTTC CAGAAATCAG TAATGCCTCT
    GTGAATGTCC TGGTACAGAA TTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA
    GATGGTCAGC TCCTGGCAGC CTTCATCCCG AGCAGCCAGA GGGGCTTTCC TGATGAAGGC
    ATCCTGGCAG TGTATTCCCT GGCCCCCCAT AACTTGGGGG AAATGCTCTA CACCAAGCGA
    TTTGGTCCCA ATGCCATTTC TGTAAGCCTG TCCCCGATGG GCCGATACGT TATGGTGGGC
    TTGGCCTCAC GAAGGATCCT ACTGCACCCC TCCACAGAGC ACATGGTAGC CCAGGTCTTC
    AGGCTGCAAC AGGCCCATGG TGGTGAGACT TCCATGAGGA GAGTTTTCAA TGTCCTTTAC
    CCCATGCCTG CAGACCAACG GAGACACGTC AGCATCAACT CTGCCCGTTG GCTACCCGAG
    CCAGGGCTTG GTTTGGCCTA TGGCACCAAC AAAGGAGACT TGGTGATCTG CCGACCAGAG
    GCCTTAAACT CTGGCATCGA GTACTACTGG GACCAGCTAA GTGAAACAGT CTTCACTGTC
    CACTCAAGTA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACGTG GCGGACAGAC
    AGAGACATGG GTTTGATGAA TGCAATTGGG CTGCAGCCTC GGAACCCAAC CACCTCTGTG
    ACATCTCAGG GCACCCAGAC TCTGGCCCTC CAGTTGCAGA ACGCTGAAAC ACAGACTGAG
    AGGGAGGAAG AGGAACCAGG GGCAGCATCT TCAGGTCCTG GTGAAGGCGA GGGCTCAGAA
    TATGGTGGCA GTGGGGAAGA TGCCCTCAGC AGGATCCAGA GGCTGATGGC CGAGGGTGGC
    ATGACAGCTG TGGTACAACG GGAACAGAGC ACCACCATGG CCTCCATGGG TGGCTTTGGC
    AACAACATCA TCGTTAGCCA CCGCATTCAC CGCAGCTCCC AGACAGGCAC AGAGTCAGGG
    GCTGCTCGTA CCTCCTCACC CCAGCCCTCC ACCTCTCGAG GACTGCCATC AGAACCTGGG
    CAACTGGCAG AGCGAGCGCT AAGCCCCCGG ACAGCCTCCT GGGACCAGCC TAGTACCTCT
    GGGAGGGAAC TACCCCAGCC AGCCCTGTCC TCTTCCTCCC CTGTCCCCAT TCCAGTCCCC
    CTTGCCAGCA ATGAGGGACC AACCATGCAC TGCAATGTGA CCAATAACAG TCACCTTCCT
    GAGGGTGATG GTAGCAACAG GGGGGAAGCT GCAGGCCCTA GTGGAGAGCC ACAGAACCGA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_766257.3
    CDS362..4264
    Translation

    Target ORF information:

    RefSeq Version NM_172669.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus autophagy/beclin 1 regulator 1 (Ambra1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_172669.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAAGTTG TCCCAGAGAA GAATGCTGTA CGAATCCTCT GGGGCCGAGA ACGGGGCACT 
    CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCT GATAGTCCAC GCTCGACCTT CTTACTGGCC
    TTCAGCCCAG ACAGGACTCT CTTGGCATCC ACACATGTGA ACCATAACAT CTATATTACA
    GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATCGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ACCCCACCAT CTCAGGCCTT ATTGCTTCTG GTTGCCTAGA TGGGGAAGTT
    AGGATTTGGG ATTTACATGG GGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATA
    GCCTCCTTGG CTTTCCACCC TACGGCTCAG CTTCTACTAA TTGCCACTGC AAATGAGATC
    CATTTCTGGG ATTGGAGTCG TCGGGAGCCC TTTGCTGTGG TGAAAACAGC CAGTGAGATG
    GAGCGTGTCC GCCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTTAC AGCTATTGTT
    AACCCTTCTA ATCAGCAGGG TGATGACGAA CCAGAAATCC CTATAGATGG AACAGAGTTA
    TCTCATTACC GTCAACGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGT TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC
    ACAGTACAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG
    CGTCCCAGGA CTTCCGCTTA CATCAGACTC CGACAGCGGG TCAGTTACCC CACCACAGTT
    GAGTGCTGCC AGCACCCTGG GATCCTGTGC CTTTGCAGCC GCTGTGCTGG CACTCGAGTT
    CCTTCCCTCT TGCCACACCA GGACAGTGTC CCCCCTGCTT CTGCCAGAGC TACTACCCCT
    TCCTTTTCTT TTGTACAGAC CGAGCCCTTC CATCCCCCGG AGCAGGCTTC ATCAACGCAG
    CAGGACCAGG GCCTCCTGAA CCGGCCGTCT GCCTTCAGTA CAGTCCAGAG CAGCACTGCG
    GGCAACACGC TCCGCAACCT CAGTCTGGGT CCTACCCGGC GCTCTTTGGG GGGCCCTTTG
    TCTAGCCACC CTTCTAGGTA TCACAGAGAA CTAGCCCCTG GGCTGACAGG TTCTGAGTGG
    ACCCGGACAG TACTCACTCT GAACTCTCGT TCTGAGGTAG AATCTATGCC CCCACCGAGG
    ACCAGTGCCT CCTCAGTGAG TTTGCTTTCT GTACTGAGAC AGCAGGAAGG TGGCTCTCAG
    GCATCTGTGT ATACTTCAGC CACAGAAGGG AGGGGTTTTC CATCATCAGG GTTGGCAACT
    GAGTCAGATG GAGGGAATGG TTCCAGCCAA AACAACTCAG GCAGCATTCG CCATGAGCTT
    CAATGTGACC TGAGACGCTT CTTTTTGGAG TATGACAGGC TGCAGGAGCT GGATCAGAGC
    CTGAGTGGGG AAACTCCCCA GACCCAACAG GCCCAGGAAA TGCTCAACAA TAATATTGAA
    TCTGAGAGAC CAGGTCCTTC CCACCTGCCT ACCCCACACA GCAGTGAGAA CAACTCCAAC
    CTGTCTCGGG GCCACCTTAA TCGATGCCGT GCTTGCCATA ATCTTCTAAC CTTCAACAAT
    GATACCCTTC GTTGGGAAAG AACCACACCT AACTACTCAT CTGGTGAGGC TAGCTCTTCC
    TGGCACGTCT CCACTACCTT TGAGGGCATG CCACCAAGTG GCAACCAGCT ACCACCTCTT
    GAGCGGACTG AGGGCCAAAT GCCTAGCTCC AGCAGGCTGG AGTTGAGCAG CTCTGCTAGT
    TCACAAGAGG AGAGGACTGT GGGGGTGGCC TTTAACCAGG AGACAGGCCA CTGGGAACGC
    ATTTATACCC AGTCCAGCAG ATCTGGAACT GTATCACAGG AGGCCTTACA TCAAGATATG
    CCTGAAGAAA GCTCTGAGGA AGACTCCCTC AGGAGGAGGC TGCTGGAGTC TTCCCTCATT
    TCATTATCCC GTTATGATGG AGCAGGATCC AGAGAGCACC CAATTTACCC AGACCCAGCG
    AGATTATCTC CTGCTGCATA CTACGCCCAG AGGATGATCC AGTATCTCTC AAGGAGAGAC
    AGTATTCGCC AGCGCTCCAT GCGCTACCAA CAGAATCGGC TTCGTTCTTC CACCTCCTCT
    TCTTCCTCAG ACAACCAGGG TCCATCAGTA GAGGGAACCG ACTTGGAATT TGAGGACTTT
    GAGGACAATG GTGACAGATC TAGGCACCGA GCTCCCCGAA ATGCCCGAAT GTCTGCACCT
    TCACTTGGAC GATTTGTTCC AAGGCGTTTT TTGCTGCCTG AGTACTTGCC TTATGCTGGG
    ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCATT CTTCTGTTAA CAGGGTCCTG
    GCAGGGGCAG TAATTGGAGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC
    TACAGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTTC CAGAAATCAG TAATGCCTCT
    GTGAATGTCC TGGTACAGAA TTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA
    GATGGTCAGC TCCTGGCAGC CTTCATCCCG AGCAGCCAGA GGGGCTTTCC TGATGAAGGC
    ATCCTGGCAG TGTATTCCCT GGCCCCCCAT AACTTGGGGG AAATGCTCTA CACCAAGCGA
    TTTGGTCCCA ATGCCATTTC TGTAAGCCTG TCCCCGATGG GCCGATACGT TATGGTGGGC
    TTGGCCTCAC GAAGGATCCT ACTGCACCCC TCCACAGAGC ACATGGTAGC CCAGGTCTTC
    AGGCTGCAAC AGGCCCATGG TGGTGAGACT TCCATGAGGA GAGTTTTCAA TGTCCTTTAC
    CCCATGCCTG CAGACCAACG GAGACACGTC AGCATCAACT CTGCCCGTTG GCTACCCGAG
    CCAGGGCTTG GTTTGGCCTA TGGCACCAAC AAAGGAGACT TGGTGATCTG CCGACCAGAG
    GCCTTAAACT CTGGCATCGA GTACTACTGG GACCAGCTAA GTGAAACAGT CTTCACTGTC
    CACTCAAGTA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACGTG GCGGACAGAC
    AGAGACATGG GTTTGATGAA TGCAATTGGG CTGCAGCCTC GGAACCCAAC CACCTCTGTG
    ACATCTCAGG GCACCCAGAC TCTGGCCCTC CAGTTGCAGA ACGCTGAAAC ACAGACTGAG
    AGGGAGGAAG AGGAACCAGG GGCAGCATCT TCAGGTCCTG GTGAAGGCGA GGGCTCAGAA
    TATGGTGGCA GTGGGGAAGA TGCCCTCAGC AGGATCCAGA GGCTGATGGC CGAGGGTGGC
    ATGACAGCTG TGGTACAACG GGAACAGAGC ACCACCATGG CCTCCATGGG TGGCTTTGGC
    AACAACATCA TCGTTAGCCA CCGCATTCAC CGCAGCTCCC AGACAGGCAC AGAGTCAGGG
    GCTGCTCGTA CCTCCTCACC CCAGCCCTCC ACCTCTCGAG GACTGCCATC AGAACCTGGG
    CAACTGGCAG AGCGAGCGCT AAGCCCCCGG ACAGCCTCCT GGGACCAGCC TAGTACCTCT
    GGGAGGGAAC TACCCCAGCC AGCCCTGTCC TCTTCCTCCC CTGTCCCCAT TCCAGTCCCC
    CTTGCCAGCA ATGAGGGACC AACCATGCAC TGCAATGTGA CCAATAACAG TCACCTTCCT
    GAGGGTGATG GTAGCAACAG GGGGGAAGCT GCAGGCCCTA GTGGAGAGCC ACAGAACCGA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu18871
    Clone ID Related Accession (Same CDS sequence) NM_001080754.1
    Accession Version NM_001080754.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3630bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-26
    Organism Mus musculus(house mouse)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ150588.1, AK169280.1 and BC032293.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC032293.1, SRR1660813.114170.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAAAGTTG TCCCAGAGAA GAATGCTGTA CGAATCCTCT GGGGCCGAGA ACGGGGCACT 
    CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCT GATAGTCCAC GCTCGACCTT CTTACTGGCC
    TTCAGCCCAG ACAGGACTCT CTTGGCATCC ACACATGTGA ACCATAACAT CTATATTACA
    GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATCGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ACCCCACCAT CTCAGGCCTT ATTGCTTCTG GTTGCCTAGA TGGGGAAGTT
    AGGATTTGGG ATTTACATGG GGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATA
    GCCTCCTTGG CTTTCCACCC TACGGCTCAG CTTCTACTAA TTGCCACTGC AAATGAGATC
    CATTTCTGGG ATTGGAGTCG TCGGGAGCCC TTTGCTGTGG TGAAAACAGC CAGTGAGATG
    GAGCGTGTCC GCCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTTAC AGCTATTGTT
    AACCCTTCTA ATCAGCAGGG TGATGACGAA CCAGAAATCC CTATAGATGG AACAGAGTTA
    TCTCATTACC GTCAACGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGT TCCTCTGGCA TCCAGACCGA GCCCTTCCAT
    CCCCCGGAGC AGGCTTCATC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC
    TTCAGTACAG TCCAGAGCAG CACTGCGGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT
    ACCCGGCGCT CTTTGGGGGG CCCTTTGTCT AGCCACCCTT CTAGGTATCA CAGAGAACTA
    GCCCCTGGGC TGACAGGTTC TGAGTGGACC CGGACAGTAC TCACTCTGAA CTCTCGTTCT
    GAGGTAGAAT CTATGCCCCC ACCGAGGACC AGTGCCTCCT CAGTGAGTTT GCTTTCTGTA
    CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTATA CTTCAGCCAC AGAAGGGAGG
    GGTTTTCCAT CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGTTC CAGCCAAAAC
    AACTCAGGCA GCATTCGCCA TGAGCTTCAA TGTGACCTGA GACGCTTCTT TTTGGAGTAT
    GACAGGCTGC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAA CTCCCCAGAC CCAACAGGCC
    CAGGAAATGC TCAACAATAA TATTGAATCT GAGAGACCAG GTCCTTCCCA CCTGCCTACC
    CCACACAGCA GTGAGAACAA CTCCAACCTG TCTCGGGGCC ACCTTAATCG ATGCCGTGCT
    TGCCATAATC TTCTAACCTT CAACAATGAT ACCCTTCGTT GGGAAAGAAC CACACCTAAC
    TACTCATCTG GTGAGGCTAG CTCTTCCTGG CACGTCTCCA CTACCTTTGA GGGCATGCCA
    CCAAGTGGCA ACCAGCTACC ACCTCTTGAG CGGACTGAGG GCCAAATGCC TAGCTCCAGC
    AGGCTGGAGT TGAGCAGCTC TGCTAGTTCA CAAGAGGAGA GGACTGTGGG GGTGGCCTTT
    AACCAGGAGA CAGGCCACTG GGAACGCATT TATACCCAGT CCAGCAGATC TGGAACTGTA
    TCACAGGAGG CCTTACATCA AGATATGCCT GAAGAAAGCT CTGAGGAAGA CTCCCTCAGG
    AGGAGGCTGC TGGAGTCTTC CCTCATTTCA TTATCCCGTT ATGATGGAGC AGGATCCAGA
    GAGCACCCAA TTTACCCAGA CCCAGCGAGA TTATCTCCTG CTGCATACTA CGCCCAGAGG
    ATGATCCAGT ATCTCTCAAG GAGAGACAGT ATTCGCCAGC GCTCCATGCG CTACCAACAG
    AATCGGCTTC GTTCTTCCAC CTCCTCTTCT TCCTCAGACA ACCAGGGTCC ATCAGTAGAG
    GGAACCGACT TGGAATTTGA GGACTTTGAG GACAATGGTG ACAGATCTAG GCACCGAGCT
    CCCCGAAATG CCCGAATGTC TGCACCTTCA CTTGGACGAT TTGTTCCAAG GCGTTTTTTG
    CTGCCTGAGT ACTTGCCTTA TGCTGGGATT TTTCATGAAC GTGGACAGCC TGGCTTGGCT
    ACTCATTCTT CTGTTAACAG GGTCCTGGCA GGGGCAGTAA TTGGAGATGG ACAGTCTGCT
    GTGGCCAGTA ACATTGCCAA TACTACCTAC AGGCTCCAGT GGTGGGACTT CACTAAGTTT
    GACCTTCCAG AAATCAGTAA TGCCTCTGTG AATGTCCTGG TACAGAATTG CAAGATCTAC
    AATGATGCCA GCTGTGACAT TTCTGCAGAT GGTCAGCTCC TGGCAGCCTT CATCCCGAGC
    AGCCAGAGGG GCTTTCCTGA TGAAGGCATC CTGGCAGTGT ATTCCCTGGC CCCCCATAAC
    TTGGGGGAAA TGCTCTACAC CAAGCGATTT GGTCCCAATG CCATTTCTGT AAGCCTGTCC
    CCGATGGGCC GATACGTTAT GGTGGGCTTG GCCTCACGAA GGATCCTACT GCACCCCTCC
    ACAGAGCACA TGGTAGCCCA GGTCTTCAGG CTGCAACAGG CCCATGGTGG TGAGACTTCC
    ATGAGGAGAG TTTTCAATGT CCTTTACCCC ATGCCTGCAG ACCAACGGAG ACACGTCAGC
    ATCAACTCTG CCCGTTGGCT ACCCGAGCCA GGGCTTGGTT TGGCCTATGG CACCAACAAA
    GGAGACTTGG TGATCTGCCG ACCAGAGGCC TTAAACTCTG GCATCGAGTA CTACTGGGAC
    CAGCTAAGTG AAACAGTCTT CACTGTCCAC TCAAGTAGCA GGAGCAGCGA GCGGCCTGGA
    ACCAGCAGAG CCACGTGGCG GACAGACAGA GACATGGGTT TGATGAATGC AATTGGGCTG
    CAGCCTCGGA ACCCAACCAC CTCTGTGACA TCTCAGGGCA CCCAGACTCT GGCCCTCCAG
    TTGCAGAACG CTGAAACACA GACTGAGAGG GAGGAAGAGG AACCAGGGGC AGCATCTTCA
    GGTCCTGGTG AAGGCGAGGG CTCAGAATAT GGTGGCAGTG GGGAAGATGC CCTCAGCAGG
    ATCCAGAGGC TGATGGCCGA GGGTGGCATG ACAGCTGTGG TACAACGGGA ACAGAGCACC
    ACCATGGCCT CCATGGGTGG CTTTGGCAAC AACATCATCG TTAGCCACCG CATTCACCGC
    AGCTCCCAGA CAGGCACAGA GTCAGGGGCT GCTCGTACCT CCTCACCCCA GCCCTCCACC
    TCTCGAGGAC TGCCATCAGA ACCTGGGCAA CTGGCAGAGC GAGCGCTAAG CCCCCGGACA
    GCCTCCTGGG ACCAGCCTAG TACCTCTGGG AGGGAACTAC CCCAGCCAGC CCTGTCCTCT
    TCCTCCCCTG TCCCCATTCC AGTCCCCCTT GCCAGCAATG AGGGACCAAC CATGCACTGC
    AATGTGACCA ATAACAGTCA CCTTCCTGAG GGTGATGGTA GCAACAGGGG GGAAGCTGCA
    GGCCCTAGTG GAGAGCCACA GAACCGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001074223.1
    CDS362..3991
    Translation

    Target ORF information:

    RefSeq Version NM_001080754.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus autophagy/beclin 1 regulator 1 (Ambra1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001080754.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAAAGTTG TCCCAGAGAA GAATGCTGTA CGAATCCTCT GGGGCCGAGA ACGGGGCACT 
    CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCT GATAGTCCAC GCTCGACCTT CTTACTGGCC
    TTCAGCCCAG ACAGGACTCT CTTGGCATCC ACACATGTGA ACCATAACAT CTATATTACA
    GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATCGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ACCCCACCAT CTCAGGCCTT ATTGCTTCTG GTTGCCTAGA TGGGGAAGTT
    AGGATTTGGG ATTTACATGG GGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATA
    GCCTCCTTGG CTTTCCACCC TACGGCTCAG CTTCTACTAA TTGCCACTGC AAATGAGATC
    CATTTCTGGG ATTGGAGTCG TCGGGAGCCC TTTGCTGTGG TGAAAACAGC CAGTGAGATG
    GAGCGTGTCC GCCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTTAC AGCTATTGTT
    AACCCTTCTA ATCAGCAGGG TGATGACGAA CCAGAAATCC CTATAGATGG AACAGAGTTA
    TCTCATTACC GTCAACGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGT TCCTCTGGCA TCCAGACCGA GCCCTTCCAT
    CCCCCGGAGC AGGCTTCATC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC
    TTCAGTACAG TCCAGAGCAG CACTGCGGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT
    ACCCGGCGCT CTTTGGGGGG CCCTTTGTCT AGCCACCCTT CTAGGTATCA CAGAGAACTA
    GCCCCTGGGC TGACAGGTTC TGAGTGGACC CGGACAGTAC TCACTCTGAA CTCTCGTTCT
    GAGGTAGAAT CTATGCCCCC ACCGAGGACC AGTGCCTCCT CAGTGAGTTT GCTTTCTGTA
    CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTATA CTTCAGCCAC AGAAGGGAGG
    GGTTTTCCAT CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGTTC CAGCCAAAAC
    AACTCAGGCA GCATTCGCCA TGAGCTTCAA TGTGACCTGA GACGCTTCTT TTTGGAGTAT
    GACAGGCTGC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAA CTCCCCAGAC CCAACAGGCC
    CAGGAAATGC TCAACAATAA TATTGAATCT GAGAGACCAG GTCCTTCCCA CCTGCCTACC
    CCACACAGCA GTGAGAACAA CTCCAACCTG TCTCGGGGCC ACCTTAATCG ATGCCGTGCT
    TGCCATAATC TTCTAACCTT CAACAATGAT ACCCTTCGTT GGGAAAGAAC CACACCTAAC
    TACTCATCTG GTGAGGCTAG CTCTTCCTGG CACGTCTCCA CTACCTTTGA GGGCATGCCA
    CCAAGTGGCA ACCAGCTACC ACCTCTTGAG CGGACTGAGG GCCAAATGCC TAGCTCCAGC
    AGGCTGGAGT TGAGCAGCTC TGCTAGTTCA CAAGAGGAGA GGACTGTGGG GGTGGCCTTT
    AACCAGGAGA CAGGCCACTG GGAACGCATT TATACCCAGT CCAGCAGATC TGGAACTGTA
    TCACAGGAGG CCTTACATCA AGATATGCCT GAAGAAAGCT CTGAGGAAGA CTCCCTCAGG
    AGGAGGCTGC TGGAGTCTTC CCTCATTTCA TTATCCCGTT ATGATGGAGC AGGATCCAGA
    GAGCACCCAA TTTACCCAGA CCCAGCGAGA TTATCTCCTG CTGCATACTA CGCCCAGAGG
    ATGATCCAGT ATCTCTCAAG GAGAGACAGT ATTCGCCAGC GCTCCATGCG CTACCAACAG
    AATCGGCTTC GTTCTTCCAC CTCCTCTTCT TCCTCAGACA ACCAGGGTCC ATCAGTAGAG
    GGAACCGACT TGGAATTTGA GGACTTTGAG GACAATGGTG ACAGATCTAG GCACCGAGCT
    CCCCGAAATG CCCGAATGTC TGCACCTTCA CTTGGACGAT TTGTTCCAAG GCGTTTTTTG
    CTGCCTGAGT ACTTGCCTTA TGCTGGGATT TTTCATGAAC GTGGACAGCC TGGCTTGGCT
    ACTCATTCTT CTGTTAACAG GGTCCTGGCA GGGGCAGTAA TTGGAGATGG ACAGTCTGCT
    GTGGCCAGTA ACATTGCCAA TACTACCTAC AGGCTCCAGT GGTGGGACTT CACTAAGTTT
    GACCTTCCAG AAATCAGTAA TGCCTCTGTG AATGTCCTGG TACAGAATTG CAAGATCTAC
    AATGATGCCA GCTGTGACAT TTCTGCAGAT GGTCAGCTCC TGGCAGCCTT CATCCCGAGC
    AGCCAGAGGG GCTTTCCTGA TGAAGGCATC CTGGCAGTGT ATTCCCTGGC CCCCCATAAC
    TTGGGGGAAA TGCTCTACAC CAAGCGATTT GGTCCCAATG CCATTTCTGT AAGCCTGTCC
    CCGATGGGCC GATACGTTAT GGTGGGCTTG GCCTCACGAA GGATCCTACT GCACCCCTCC
    ACAGAGCACA TGGTAGCCCA GGTCTTCAGG CTGCAACAGG CCCATGGTGG TGAGACTTCC
    ATGAGGAGAG TTTTCAATGT CCTTTACCCC ATGCCTGCAG ACCAACGGAG ACACGTCAGC
    ATCAACTCTG CCCGTTGGCT ACCCGAGCCA GGGCTTGGTT TGGCCTATGG CACCAACAAA
    GGAGACTTGG TGATCTGCCG ACCAGAGGCC TTAAACTCTG GCATCGAGTA CTACTGGGAC
    CAGCTAAGTG AAACAGTCTT CACTGTCCAC TCAAGTAGCA GGAGCAGCGA GCGGCCTGGA
    ACCAGCAGAG CCACGTGGCG GACAGACAGA GACATGGGTT TGATGAATGC AATTGGGCTG
    CAGCCTCGGA ACCCAACCAC CTCTGTGACA TCTCAGGGCA CCCAGACTCT GGCCCTCCAG
    TTGCAGAACG CTGAAACACA GACTGAGAGG GAGGAAGAGG AACCAGGGGC AGCATCTTCA
    GGTCCTGGTG AAGGCGAGGG CTCAGAATAT GGTGGCAGTG GGGAAGATGC CCTCAGCAGG
    ATCCAGAGGC TGATGGCCGA GGGTGGCATG ACAGCTGTGG TACAACGGGA ACAGAGCACC
    ACCATGGCCT CCATGGGTGG CTTTGGCAAC AACATCATCG TTAGCCACCG CATTCACCGC
    AGCTCCCAGA CAGGCACAGA GTCAGGGGCT GCTCGTACCT CCTCACCCCA GCCCTCCACC
    TCTCGAGGAC TGCCATCAGA ACCTGGGCAA CTGGCAGAGC GAGCGCTAAG CCCCCGGACA
    GCCTCCTGGG ACCAGCCTAG TACCTCTGGG AGGGAACTAC CCCAGCCAGC CCTGTCCTCT
    TCCTCCCCTG TCCCCATTCC AGTCCCCCTT GCCAGCAATG AGGGACCAAC CATGCACTGC
    AATGTGACCA ATAACAGTCA CCTTCCTGAG GGTGATGGTA GCAACAGGGG GGAAGCTGCA
    GGCCCTAGTG GAGAGCCACA GAACCGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu30300
    Clone ID Related Accession (Same CDS sequence) XM_006499252.1 , XM_006499252.2
    Accession Version XM_006499252.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3816bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAAAGTTG TCCCAGAGAA GAATGCTGTA CGAATCCTCT GGGGCCGAGA ACGGGGCACT 
    CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCT GATAGTCCAC GCTCGACCTT CTTACTGGCC
    TTCAGCCCAG ACAGGACTCT CTTGGCATCC ACACATGTGA ACCATAACAT CTATATTACA
    GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATCGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ACCCCACCAT CTCAGGCCTT ATTGCTTCTG GTTGCCTAGA TGGGGAAGTT
    AGGATTTGGG ATTTACATGG GGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATA
    GCCTCCTTGG CTTTCCACCC TACGGCTCAG CTTCTACTAA TTGCCACTGC AAATGAGATC
    CATTTCTGGG ATTGGAGTCG TCGGGAGCCC TTTGCTGTGG TGAAAACAGC CAGTGAGATG
    GAGCGTGTCC GCCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTTAC AGCTATTGTT
    AACCCTTCTA ATCAGCAGGG TGATGACGAA CCAGAAATCC CTATAGATGG AACAGAGTTA
    TCTCATTACC GTCAACGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGT TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC
    ACAGTACAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG
    CGTCCCAGGA CTTCCGCTTA CATCAGACTC CGACAGCGGG TCAGTTACCC CACCACAGTT
    GAGTGCTGCC AGCACCCTGG GATCCTGTGC CTTTGCAGCC GCTGTGCTGG CACTCGAGTT
    CCTTCCCTCT TGCCACACCA GGACAGTGTC CCCCCTGCTT CTGCCAGAGC TACTACCCCT
    TCCTTTTCTT TTGTACAGAC CGAGCCCTTC CATCCCCCGG AGCAGGCTTC ATCAACGCAG
    CAGGACCAGG GCCTCCTGAA CCGGCCGTCT GCCTTCAGTA CAGTCCAGAG CAGCACTGCG
    GGCAACACGC TCCGCAACCT CAGTCTGGGT CCTACCCGGC GCTCTTTGGG GGGCCCTTTG
    TCTAGCCACC CTTCTAGGTA TCACAGAGAA CTAGCCCCTG GGCTGACAGG TTCTGAGTGG
    ACCCGGACAG TACTCACTCT GAACTCTCGT TCTGAGGTAG AATCTATGCC CCCACCGAGG
    ACCAGTGCCT CCTCAGTGAG TTTGCTTTCT GTACTGAGAC AGCAGGAAGG TGGCTCTCAG
    GCATCTGTGT ATACTTCAGC CACAGAAGGG AGGGGTTTTC CATCATCAGG GTTGGCAACT
    GAGTCAGATG GAGGGAATGG TTCCAGCCAA AACAACTCAG GCAGCATTCG CCATGAGCTT
    CAATGTGACC TGAGACGCTT CTTTTTGGAG TATGACAGGC TGCAGGAGCT GGATCAGAGC
    CTGAGTGGGG AAACTCCCCA GACCCAACAG GCCCAGGAAA TGCTCAACAA TAATATTGAA
    TCTGAGAGAC CAGGTCCTTC CCACCTGCCT ACCCCACACA GCAGTGAGAA CAACTCCAAC
    CTGTCTCGGG GCCACCTTAA TCGATGCCGT GCTTGCCATA ATCTTCTAAC CTTCAACAAT
    GATACCCTTC GTTGGGAAAG AACCACACCT AACTACTCAT CTGGTGAGGC TAGCTCTTCC
    TGGCACGTCT CCACTACCTT TGAGGGCATG CCACCAAGTG GCAACCAGCT ACCACCTCTT
    GAGCGGACTG AGGGCCAAAT GCCTAGCTCC AGCAGGCTGG AGTTGAGCAG CTCTGCTAGT
    TCACAAGAGG AGAGGACTGT GGGGGTGGCC TTTAACCAGG AGACAGGCCA CTGGGAACGC
    ATTTATACCC AGTCCAGCAG ATCTGGAACT GTATCACAGG AGGCCTTACA TCAAGATATG
    CCTGAAGAAA GCTCTGAGGA AGACTCCCTC AGGAGATTAT CTCCTGCTGC ATACTACGCC
    CAGAGGATGA TCCAGTATCT CTCAAGGAGA GACAGTATTC GCCAGCGCTC CATGCGCTAC
    CAACAGAATC GGCTTCGTTC TTCCACCTCC TCTTCTTCCT CAGACAACCA GGGTCCATCA
    GTAGAGGGAA CCGACTTGGA ATTTGAGGAC TTTGAGGACA ATGGTGACAG ATCTAGGCAC
    CGAGCTCCCC GAAATGCCCG AATGTCTGCA CCTTCACTTG GACGATTTGT TCCAAGGCGT
    TTTTTGCTGC CTGAGTACTT GCCTTATGCT GGGATTTTTC ATGAACGTGG ACAGCCTGGC
    TTGGCTACTC ATTCTTCTGT TAACAGGGTC CTGGCAGGGG CAGTAATTGG AGATGGACAG
    TCTGCTGTGG CCAGTAACAT TGCCAATACT ACCTACAGGC TCCAGTGGTG GGACTTCACT
    AAGTTTGACC TTCCAGAAAT CAGTAATGCC TCTGTGAATG TCCTGGTACA GAATTGCAAG
    ATCTACAATG ATGCCAGCTG TGACATTTCT GCAGATGGTC AGCTCCTGGC AGCCTTCATC
    CCGAGCAGCC AGAGGGGCTT TCCTGATGAA GGCATCCTGG CAGTGTATTC CCTGGCCCCC
    CATAACTTGG GGGAAATGCT CTACACCAAG CGATTTGGTC CCAATGCCAT TTCTGTAAGC
    CTGTCCCCGA TGGGCCGATA CGTTATGGTG GGCTTGGCCT CACGAAGGAT CCTACTGCAC
    CCCTCCACAG AGCACATGGT AGCCCAGGTC TTCAGGCTGC AACAGGCCCA TGGTGGTGAG
    ACTTCCATGA GGAGAGTTTT CAATGTCCTT TACCCCATGC CTGCAGACCA ACGGAGACAC
    GTCAGCATCA ACTCTGCCCG TTGGCTACCC GAGCCAGGGC TTGGTTTGGC CTATGGCACC
    AACAAAGGAG ACTTGGTGAT CTGCCGACCA GAGGCCTTAA ACTCTGGCAT CGAGTACTAC
    TGGGACCAGC TAAGTGAAAC AGTCTTCACT GTCCACTCAA GTAGCAGGAG CAGCGAGCGG
    CCTGGAACCA GCAGAGCCAC GTGGCGGACA GACAGAGACA TGGGTTTGAT GAATGCAATT
    GGGCTGCAGC CTCGGAACCC AACCACCTCT GTGACATCTC AGGGCACCCA GACTCTGGCC
    CTCCAGTTGC AGAACGCTGA AACACAGACT GAGAGGGAGG AAGAGGAACC AGGGGCAGCA
    TCTTCAGGTC CTGGTGAAGG CGAGGGCTCA GAATATGGTG GCAGTGGGGA AGATGCCCTC
    AGCAGGATCC AGAGGCTGAT GGCCGAGGGT GGCATGACAG CTGTGGTACA ACGGGAACAG
    AGCACCACCA TGGCCTCCAT GGGTGGCTTT GGCAACAACA TCATCGTTAG CCACCGCATT
    CACCGCAGCT CCCAGACAGG CACAGAGTCA GGGGCTGCTC GTACCTCCTC ACCCCAGCCC
    TCCACCTCTC GAGGACTGCC ATCAGAACCT GGGCAACTGG CAGAGCGAGC GCTAAGCCCC
    CGGACAGCCT CCTGGGACCA GCCTAGTACC TCTGGGAGGG AACTACCCCA GCCAGCCCTG
    TCCTCTTCCT CCCCTGTCCC CATTCCAGTC CCCCTTGCCA GCAATGAGGG ACCAACCATG
    CACTGCAATG TGACCAATAA CAGTCACCTT CCTGAGGGTG ATGGTAGCAA CAGGGGGGAA
    GCTGCAGGCC CTAGTGGAGA GCCACAGAAC CGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006499315.1
    CDS389..4204
    Translation

    Target ORF information:

    RefSeq Version XM_006499252.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus autophagy/beclin 1 regulator 1 (Ambra1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006499252.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAAAGTTG TCCCAGAGAA GAATGCTGTA CGAATCCTCT GGGGCCGAGA ACGGGGCACT 
    CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCT GATAGTCCAC GCTCGACCTT CTTACTGGCC
    TTCAGCCCAG ACAGGACTCT CTTGGCATCC ACACATGTGA ACCATAACAT CTATATTACA
    GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATCGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ACCCCACCAT CTCAGGCCTT ATTGCTTCTG GTTGCCTAGA TGGGGAAGTT
    AGGATTTGGG ATTTACATGG GGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATA
    GCCTCCTTGG CTTTCCACCC TACGGCTCAG CTTCTACTAA TTGCCACTGC AAATGAGATC
    CATTTCTGGG ATTGGAGTCG TCGGGAGCCC TTTGCTGTGG TGAAAACAGC CAGTGAGATG
    GAGCGTGTCC GCCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTTAC AGCTATTGTT
    AACCCTTCTA ATCAGCAGGG TGATGACGAA CCAGAAATCC CTATAGATGG AACAGAGTTA
    TCTCATTACC GTCAACGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGT TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC
    ACAGTACAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG
    CGTCCCAGGA CTTCCGCTTA CATCAGACTC CGACAGCGGG TCAGTTACCC CACCACAGTT
    GAGTGCTGCC AGCACCCTGG GATCCTGTGC CTTTGCAGCC GCTGTGCTGG CACTCGAGTT
    CCTTCCCTCT TGCCACACCA GGACAGTGTC CCCCCTGCTT CTGCCAGAGC TACTACCCCT
    TCCTTTTCTT TTGTACAGAC CGAGCCCTTC CATCCCCCGG AGCAGGCTTC ATCAACGCAG
    CAGGACCAGG GCCTCCTGAA CCGGCCGTCT GCCTTCAGTA CAGTCCAGAG CAGCACTGCG
    GGCAACACGC TCCGCAACCT CAGTCTGGGT CCTACCCGGC GCTCTTTGGG GGGCCCTTTG
    TCTAGCCACC CTTCTAGGTA TCACAGAGAA CTAGCCCCTG GGCTGACAGG TTCTGAGTGG
    ACCCGGACAG TACTCACTCT GAACTCTCGT TCTGAGGTAG AATCTATGCC CCCACCGAGG
    ACCAGTGCCT CCTCAGTGAG TTTGCTTTCT GTACTGAGAC AGCAGGAAGG TGGCTCTCAG
    GCATCTGTGT ATACTTCAGC CACAGAAGGG AGGGGTTTTC CATCATCAGG GTTGGCAACT
    GAGTCAGATG GAGGGAATGG TTCCAGCCAA AACAACTCAG GCAGCATTCG CCATGAGCTT
    CAATGTGACC TGAGACGCTT CTTTTTGGAG TATGACAGGC TGCAGGAGCT GGATCAGAGC
    CTGAGTGGGG AAACTCCCCA GACCCAACAG GCCCAGGAAA TGCTCAACAA TAATATTGAA
    TCTGAGAGAC CAGGTCCTTC CCACCTGCCT ACCCCACACA GCAGTGAGAA CAACTCCAAC
    CTGTCTCGGG GCCACCTTAA TCGATGCCGT GCTTGCCATA ATCTTCTAAC CTTCAACAAT
    GATACCCTTC GTTGGGAAAG AACCACACCT AACTACTCAT CTGGTGAGGC TAGCTCTTCC
    TGGCACGTCT CCACTACCTT TGAGGGCATG CCACCAAGTG GCAACCAGCT ACCACCTCTT
    GAGCGGACTG AGGGCCAAAT GCCTAGCTCC AGCAGGCTGG AGTTGAGCAG CTCTGCTAGT
    TCACAAGAGG AGAGGACTGT GGGGGTGGCC TTTAACCAGG AGACAGGCCA CTGGGAACGC
    ATTTATACCC AGTCCAGCAG ATCTGGAACT GTATCACAGG AGGCCTTACA TCAAGATATG
    CCTGAAGAAA GCTCTGAGGA AGACTCCCTC AGGAGATTAT CTCCTGCTGC ATACTACGCC
    CAGAGGATGA TCCAGTATCT CTCAAGGAGA GACAGTATTC GCCAGCGCTC CATGCGCTAC
    CAACAGAATC GGCTTCGTTC TTCCACCTCC TCTTCTTCCT CAGACAACCA GGGTCCATCA
    GTAGAGGGAA CCGACTTGGA ATTTGAGGAC TTTGAGGACA ATGGTGACAG ATCTAGGCAC
    CGAGCTCCCC GAAATGCCCG AATGTCTGCA CCTTCACTTG GACGATTTGT TCCAAGGCGT
    TTTTTGCTGC CTGAGTACTT GCCTTATGCT GGGATTTTTC ATGAACGTGG ACAGCCTGGC
    TTGGCTACTC ATTCTTCTGT TAACAGGGTC CTGGCAGGGG CAGTAATTGG AGATGGACAG
    TCTGCTGTGG CCAGTAACAT TGCCAATACT ACCTACAGGC TCCAGTGGTG GGACTTCACT
    AAGTTTGACC TTCCAGAAAT CAGTAATGCC TCTGTGAATG TCCTGGTACA GAATTGCAAG
    ATCTACAATG ATGCCAGCTG TGACATTTCT GCAGATGGTC AGCTCCTGGC AGCCTTCATC
    CCGAGCAGCC AGAGGGGCTT TCCTGATGAA GGCATCCTGG CAGTGTATTC CCTGGCCCCC
    CATAACTTGG GGGAAATGCT CTACACCAAG CGATTTGGTC CCAATGCCAT TTCTGTAAGC
    CTGTCCCCGA TGGGCCGATA CGTTATGGTG GGCTTGGCCT CACGAAGGAT CCTACTGCAC
    CCCTCCACAG AGCACATGGT AGCCCAGGTC TTCAGGCTGC AACAGGCCCA TGGTGGTGAG
    ACTTCCATGA GGAGAGTTTT CAATGTCCTT TACCCCATGC CTGCAGACCA ACGGAGACAC
    GTCAGCATCA ACTCTGCCCG TTGGCTACCC GAGCCAGGGC TTGGTTTGGC CTATGGCACC
    AACAAAGGAG ACTTGGTGAT CTGCCGACCA GAGGCCTTAA ACTCTGGCAT CGAGTACTAC
    TGGGACCAGC TAAGTGAAAC AGTCTTCACT GTCCACTCAA GTAGCAGGAG CAGCGAGCGG
    CCTGGAACCA GCAGAGCCAC GTGGCGGACA GACAGAGACA TGGGTTTGAT GAATGCAATT
    GGGCTGCAGC CTCGGAACCC AACCACCTCT GTGACATCTC AGGGCACCCA GACTCTGGCC
    CTCCAGTTGC AGAACGCTGA AACACAGACT GAGAGGGAGG AAGAGGAACC AGGGGCAGCA
    TCTTCAGGTC CTGGTGAAGG CGAGGGCTCA GAATATGGTG GCAGTGGGGA AGATGCCCTC
    AGCAGGATCC AGAGGCTGAT GGCCGAGGGT GGCATGACAG CTGTGGTACA ACGGGAACAG
    AGCACCACCA TGGCCTCCAT GGGTGGCTTT GGCAACAACA TCATCGTTAG CCACCGCATT
    CACCGCAGCT CCCAGACAGG CACAGAGTCA GGGGCTGCTC GTACCTCCTC ACCCCAGCCC
    TCCACCTCTC GAGGACTGCC ATCAGAACCT GGGCAACTGG CAGAGCGAGC GCTAAGCCCC
    CGGACAGCCT CCTGGGACCA GCCTAGTACC TCTGGGAGGG AACTACCCCA GCCAGCCCTG
    TCCTCTTCCT CCCCTGTCCC CATTCCAGTC CCCCTTGCCA GCAATGAGGG ACCAACCATG
    CACTGCAATG TGACCAATAA CAGTCACCTT CCTGAGGGTG ATGGTAGCAA CAGGGGGGAA
    GCTGCAGGCC CTAGTGGAGA GCCACAGAAC CGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu30301
    Clone ID Related Accession (Same CDS sequence) XM_006499253.2 , XM_006499253.1
    Accession Version XM_006499253.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3543bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAAAGTTG TCCCAGAGAA GAATGCTGTA CGAATCCTCT GGGGCCGAGA ACGGGGCACT 
    CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCT GATAGTCCAC GCTCGACCTT CTTACTGGCC
    TTCAGCCCAG ACAGGACTCT CTTGGCATCC ACACATGTGA ACCATAACAT CTATATTACA
    GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATCGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ACCCCACCAT CTCAGGCCTT ATTGCTTCTG GTTGCCTAGA TGGGGAAGTT
    AGGATTTGGG ATTTACATGG GGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATA
    GCCTCCTTGG CTTTCCACCC TACGGCTCAG CTTCTACTAA TTGCCACTGC AAATGAGATC
    CATTTCTGGG ATTGGAGTCG TCGGGAGCCC TTTGCTGTGG TGAAAACAGC CAGTGAGATG
    GAGCGTGTCC GCCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTTAC AGCTATTGTT
    AACCCTTCTA ATCAGCAGGG TGATGACGAA CCAGAAATCC CTATAGATGG AACAGAGTTA
    TCTCATTACC GTCAACGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGT TCCTCTGGCA TCCAGACCGA GCCCTTCCAT
    CCCCCGGAGC AGGCTTCATC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC
    TTCAGTACAG TCCAGAGCAG CACTGCGGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT
    ACCCGGCGCT CTTTGGGGGG CCCTTTGTCT AGCCACCCTT CTAGGTATCA CAGAGAACTA
    GCCCCTGGGC TGACAGGTTC TGAGTGGACC CGGACAGTAC TCACTCTGAA CTCTCGTTCT
    GAGGTAGAAT CTATGCCCCC ACCGAGGACC AGTGCCTCCT CAGTGAGTTT GCTTTCTGTA
    CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTATA CTTCAGCCAC AGAAGGGAGG
    GGTTTTCCAT CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGTTC CAGCCAAAAC
    AACTCAGGCA GCATTCGCCA TGAGCTTCAA TGTGACCTGA GACGCTTCTT TTTGGAGTAT
    GACAGGCTGC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAA CTCCCCAGAC CCAACAGGCC
    CAGGAAATGC TCAACAATAA TATTGAATCT GAGAGACCAG GTCCTTCCCA CCTGCCTACC
    CCACACAGCA GTGAGAACAA CTCCAACCTG TCTCGGGGCC ACCTTAATCG ATGCCGTGCT
    TGCCATAATC TTCTAACCTT CAACAATGAT ACCCTTCGTT GGGAAAGAAC CACACCTAAC
    TACTCATCTG GTGAGGCTAG CTCTTCCTGG CACGTCTCCA CTACCTTTGA GGGCATGCCA
    CCAAGTGGCA ACCAGCTACC ACCTCTTGAG CGGACTGAGG GCCAAATGCC TAGCTCCAGC
    AGGCTGGAGT TGAGCAGCTC TGCTAGTTCA CAAGAGGAGA GGACTGTGGG GGTGGCCTTT
    AACCAGGAGA CAGGCCACTG GGAACGCATT TATACCCAGT CCAGCAGATC TGGAACTGTA
    TCACAGGAGG CCTTACATCA AGATATGCCT GAAGAAAGCT CTGAGGAAGA CTCCCTCAGG
    AGATTATCTC CTGCTGCATA CTACGCCCAG AGGATGATCC AGTATCTCTC AAGGAGAGAC
    AGTATTCGCC AGCGCTCCAT GCGCTACCAA CAGAATCGGC TTCGTTCTTC CACCTCCTCT
    TCTTCCTCAG ACAACCAGGG TCCATCAGTA GAGGGAACCG ACTTGGAATT TGAGGACTTT
    GAGGACAATG GTGACAGATC TAGGCACCGA GCTCCCCGAA ATGCCCGAAT GTCTGCACCT
    TCACTTGGAC GATTTGTTCC AAGGCGTTTT TTGCTGCCTG AGTACTTGCC TTATGCTGGG
    ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCATT CTTCTGTTAA CAGGGTCCTG
    GCAGGGGCAG TAATTGGAGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC
    TACAGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTTC CAGAAATCAG TAATGCCTCT
    GTGAATGTCC TGGTACAGAA TTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA
    GATGGTCAGC TCCTGGCAGC CTTCATCCCG AGCAGCCAGA GGGGCTTTCC TGATGAAGGC
    ATCCTGGCAG TGTATTCCCT GGCCCCCCAT AACTTGGGGG AAATGCTCTA CACCAAGCGA
    TTTGGTCCCA ATGCCATTTC TGTAAGCCTG TCCCCGATGG GCCGATACGT TATGGTGGGC
    TTGGCCTCAC GAAGGATCCT ACTGCACCCC TCCACAGAGC ACATGGTAGC CCAGGTCTTC
    AGGCTGCAAC AGGCCCATGG TGGTGAGACT TCCATGAGGA GAGTTTTCAA TGTCCTTTAC
    CCCATGCCTG CAGACCAACG GAGACACGTC AGCATCAACT CTGCCCGTTG GCTACCCGAG
    CCAGGGCTTG GTTTGGCCTA TGGCACCAAC AAAGGAGACT TGGTGATCTG CCGACCAGAG
    GCCTTAAACT CTGGCATCGA GTACTACTGG GACCAGCTAA GTGAAACAGT CTTCACTGTC
    CACTCAAGTA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACGTG GCGGACAGAC
    AGAGACATGG GTTTGATGAA TGCAATTGGG CTGCAGCCTC GGAACCCAAC CACCTCTGTG
    ACATCTCAGG GCACCCAGAC TCTGGCCCTC CAGTTGCAGA ACGCTGAAAC ACAGACTGAG
    AGGGAGGAAG AGGAACCAGG GGCAGCATCT TCAGGTCCTG GTGAAGGCGA GGGCTCAGAA
    TATGGTGGCA GTGGGGAAGA TGCCCTCAGC AGGATCCAGA GGCTGATGGC CGAGGGTGGC
    ATGACAGCTG TGGTACAACG GGAACAGAGC ACCACCATGG CCTCCATGGG TGGCTTTGGC
    AACAACATCA TCGTTAGCCA CCGCATTCAC CGCAGCTCCC AGACAGGCAC AGAGTCAGGG
    GCTGCTCGTA CCTCCTCACC CCAGCCCTCC ACCTCTCGAG GACTGCCATC AGAACCTGGG
    CAACTGGCAG AGCGAGCGCT AAGCCCCCGG ACAGCCTCCT GGGACCAGCC TAGTACCTCT
    GGGAGGGAAC TACCCCAGCC AGCCCTGTCC TCTTCCTCCC CTGTCCCCAT TCCAGTCCCC
    CTTGCCAGCA ATGAGGGACC AACCATGCAC TGCAATGTGA CCAATAACAG TCACCTTCCT
    GAGGGTGATG GTAGCAACAG GGGGGAAGCT GCAGGCCCTA GTGGAGAGCC ACAGAACCGA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006499316.1
    CDS388..3930
    Translation

    Target ORF information:

    RefSeq Version XM_006499253.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus autophagy/beclin 1 regulator 1 (Ambra1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006499253.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAAAGTTG TCCCAGAGAA GAATGCTGTA CGAATCCTCT GGGGCCGAGA ACGGGGCACT 
    CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCT GATAGTCCAC GCTCGACCTT CTTACTGGCC
    TTCAGCCCAG ACAGGACTCT CTTGGCATCC ACACATGTGA ACCATAACAT CTATATTACA
    GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATCGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ACCCCACCAT CTCAGGCCTT ATTGCTTCTG GTTGCCTAGA TGGGGAAGTT
    AGGATTTGGG ATTTACATGG GGGCAGTGAA AGCTGGTTCA CAGACAGCAA CAATGCCATA
    GCCTCCTTGG CTTTCCACCC TACGGCTCAG CTTCTACTAA TTGCCACTGC AAATGAGATC
    CATTTCTGGG ATTGGAGTCG TCGGGAGCCC TTTGCTGTGG TGAAAACAGC CAGTGAGATG
    GAGCGTGTCC GCCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTTAC AGCTATTGTT
    AACCCTTCTA ATCAGCAGGG TGATGACGAA CCAGAAATCC CTATAGATGG AACAGAGTTA
    TCTCATTACC GTCAACGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGT TCCTCTGGCA TCCAGACCGA GCCCTTCCAT
    CCCCCGGAGC AGGCTTCATC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC
    TTCAGTACAG TCCAGAGCAG CACTGCGGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT
    ACCCGGCGCT CTTTGGGGGG CCCTTTGTCT AGCCACCCTT CTAGGTATCA CAGAGAACTA
    GCCCCTGGGC TGACAGGTTC TGAGTGGACC CGGACAGTAC TCACTCTGAA CTCTCGTTCT
    GAGGTAGAAT CTATGCCCCC ACCGAGGACC AGTGCCTCCT CAGTGAGTTT GCTTTCTGTA
    CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTATA CTTCAGCCAC AGAAGGGAGG
    GGTTTTCCAT CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGTTC CAGCCAAAAC
    AACTCAGGCA GCATTCGCCA TGAGCTTCAA TGTGACCTGA GACGCTTCTT TTTGGAGTAT
    GACAGGCTGC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAA CTCCCCAGAC CCAACAGGCC
    CAGGAAATGC TCAACAATAA TATTGAATCT GAGAGACCAG GTCCTTCCCA CCTGCCTACC
    CCACACAGCA GTGAGAACAA CTCCAACCTG TCTCGGGGCC ACCTTAATCG ATGCCGTGCT
    TGCCATAATC TTCTAACCTT CAACAATGAT ACCCTTCGTT GGGAAAGAAC CACACCTAAC
    TACTCATCTG GTGAGGCTAG CTCTTCCTGG CACGTCTCCA CTACCTTTGA GGGCATGCCA
    CCAAGTGGCA ACCAGCTACC ACCTCTTGAG CGGACTGAGG GCCAAATGCC TAGCTCCAGC
    AGGCTGGAGT TGAGCAGCTC TGCTAGTTCA CAAGAGGAGA GGACTGTGGG GGTGGCCTTT
    AACCAGGAGA CAGGCCACTG GGAACGCATT TATACCCAGT CCAGCAGATC TGGAACTGTA
    TCACAGGAGG CCTTACATCA AGATATGCCT GAAGAAAGCT CTGAGGAAGA CTCCCTCAGG
    AGATTATCTC CTGCTGCATA CTACGCCCAG AGGATGATCC AGTATCTCTC AAGGAGAGAC
    AGTATTCGCC AGCGCTCCAT GCGCTACCAA CAGAATCGGC TTCGTTCTTC CACCTCCTCT
    TCTTCCTCAG ACAACCAGGG TCCATCAGTA GAGGGAACCG ACTTGGAATT TGAGGACTTT
    GAGGACAATG GTGACAGATC TAGGCACCGA GCTCCCCGAA ATGCCCGAAT GTCTGCACCT
    TCACTTGGAC GATTTGTTCC AAGGCGTTTT TTGCTGCCTG AGTACTTGCC TTATGCTGGG
    ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCATT CTTCTGTTAA CAGGGTCCTG
    GCAGGGGCAG TAATTGGAGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC
    TACAGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTTC CAGAAATCAG TAATGCCTCT
    GTGAATGTCC TGGTACAGAA TTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA
    GATGGTCAGC TCCTGGCAGC CTTCATCCCG AGCAGCCAGA GGGGCTTTCC TGATGAAGGC
    ATCCTGGCAG TGTATTCCCT GGCCCCCCAT AACTTGGGGG AAATGCTCTA CACCAAGCGA
    TTTGGTCCCA ATGCCATTTC TGTAAGCCTG TCCCCGATGG GCCGATACGT TATGGTGGGC
    TTGGCCTCAC GAAGGATCCT ACTGCACCCC TCCACAGAGC ACATGGTAGC CCAGGTCTTC
    AGGCTGCAAC AGGCCCATGG TGGTGAGACT TCCATGAGGA GAGTTTTCAA TGTCCTTTAC
    CCCATGCCTG CAGACCAACG GAGACACGTC AGCATCAACT CTGCCCGTTG GCTACCCGAG
    CCAGGGCTTG GTTTGGCCTA TGGCACCAAC AAAGGAGACT TGGTGATCTG CCGACCAGAG
    GCCTTAAACT CTGGCATCGA GTACTACTGG GACCAGCTAA GTGAAACAGT CTTCACTGTC
    CACTCAAGTA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACGTG GCGGACAGAC
    AGAGACATGG GTTTGATGAA TGCAATTGGG CTGCAGCCTC GGAACCCAAC CACCTCTGTG
    ACATCTCAGG GCACCCAGAC TCTGGCCCTC CAGTTGCAGA ACGCTGAAAC ACAGACTGAG
    AGGGAGGAAG AGGAACCAGG GGCAGCATCT TCAGGTCCTG GTGAAGGCGA GGGCTCAGAA
    TATGGTGGCA GTGGGGAAGA TGCCCTCAGC AGGATCCAGA GGCTGATGGC CGAGGGTGGC
    ATGACAGCTG TGGTACAACG GGAACAGAGC ACCACCATGG CCTCCATGGG TGGCTTTGGC
    AACAACATCA TCGTTAGCCA CCGCATTCAC CGCAGCTCCC AGACAGGCAC AGAGTCAGGG
    GCTGCTCGTA CCTCCTCACC CCAGCCCTCC ACCTCTCGAG GACTGCCATC AGAACCTGGG
    CAACTGGCAG AGCGAGCGCT AAGCCCCCGG ACAGCCTCCT GGGACCAGCC TAGTACCTCT
    GGGAGGGAAC TACCCCAGCC AGCCCTGTCC TCTTCCTCCC CTGTCCCCAT TCCAGTCCCC
    CTTGCCAGCA ATGAGGGACC AACCATGCAC TGCAATGTGA CCAATAACAG TCACCTTCCT
    GAGGGTGATG GTAGCAACAG GGGGGAAGCT GCAGGCCCTA GTGGAGAGCC ACAGAACCGA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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