The following FXN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FXN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID |
OHu22988 |
| Clone ID Related Accession (Same CDS sequence) |
NM_000144.5
, NM_000144.4
|
| Accession Version |
NM_000144.5 Latest version! |
Documents for ORF clone product in default vector |
| Sequence Information |
ORF Nucleotide Sequence (Length: 633bp)
Protein sequence
SNP |
| Vector |
pcDNA3.1+/C-(K)DYK or customized vector |
 User Manual |
| Clone information |
Clone Map |
MSDS |
| Tag on pcDNA3.1+/C-(K)DYK |
C terminal DYKDDDDK tags |
| ORF Insert Method |
CloneEZ™ Seamless cloning technology |
| Insert Structure |
linear |
| Update Date |
2019-11-10 |
| Organism |
Homo sapiens(human) |
| Product |
frataxin, mitochondrial isoform 1 preproprotein |
| Comment |
Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL162730.26, U43747.1 and BC023633.2. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_000144.4.
Transcript Variant: This variant (1) encodes the longer isoform (1).
Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.
##Evidence-Data-START##
Transcript exon combination :: BC048097.1, SRR1803611.272596.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348]
##Evidence-Data-END##
##RefSeq-Attributes-START##
gene product(s) localized to mito. :: reported by MitoCarta
MANE Ensembl match :: ENST00000484259.3/ ENSP00000419243.2
RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein
##RefSeq-Attributes-END##
COMPLETENESS: full length.
|
1
61
121
181
241
301
361
421
481
541
601
| ATGTGGACTC TCGGGCGCCG CGCAGTAGCC GGCCTCCTGG CGTCACCCAG CCCAGCCCAG
GCCCAGACCC TCACCCGGGT CCCGCGGCCG GCAGAGTTGG CCCCACTCTG CGGCCGCCGT
GGCCTGCGCA CCGACATCGA TGCGACCTGC ACGCCCCGCC GCGCAAGTTC GAACCAACGT
GGCCTCAACC AGATTTGGAA TGTCAAAAAG CAGAGTGTCT ATTTGATGAA TTTGAGGAAA
TCTGGAACTT TGGGCCACCC AGGCTCTCTA GATGAGACCA CCTATGAAAG ACTAGCAGAG
GAAACGCTGG ACTCTTTAGC AGAGTTTTTT GAAGACCTTG CAGACAAGCC ATACACGTTT
GAGGACTATG ATGTCTCCTT TGGGAGTGGT GTCTTAACTG TCAAACTGGG TGGAGATCTA
GGAACCTATG TGATCAACAA GCAGACGCCA AACAAGCAAA TCTGGCTATC TTCTCCATCC
AGTGGACCTA AGCGTTATGA CTGGACTGGG AAAAACTGGG TGTACTCCCA CGACGGCGTG
TCCCTCCATG AGCTGCTGGC CGCAGAGCTC ACTAAAGCCT TAAAAACCAA ACTGGACTTG
TCTTCCTTGG CCTATTCCGG AAAAGATGCT TGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq |
NP_000135.2 |
| CDS | 32..664 |
| Translation | |
Target ORF information:
| RefSeq Version | NM_000144.5 |
| Organism | Homo sapiens(human) |
| Definition | Homo sapiens frataxin (FXN), transcript variant 1, mRNA. |
Target ORF information:
| Epitope | DYKDDDDK |
| Bacterial selection | AMPR |
| Mammalian selection | NeoR |
| Vector | pcDNA3.1+/C-(K)DYK |
|
 NM_000144.5 |
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
| ATGTGGACTC TCGGGCGCCG CGCAGTAGCC GGCCTCCTGG CGTCACCCAG CCCAGCCCAG
GCCCAGACCC TCACCCGGGT CCCGCGGCCG GCAGAGTTGG CCCCACTCTG CGGCCGCCGT
GGCCTGCGCA CCGACATCGA TGCGACCTGC ACGCCCCGCC GCGCAAGTTC GAACCAACGT
GGCCTCAACC AGATTTGGAA TGTCAAAAAG CAGAGTGTCT ATTTGATGAA TTTGAGGAAA
TCTGGAACTT TGGGCCACCC AGGCTCTCTA GATGAGACCA CCTATGAAAG ACTAGCAGAG
GAAACGCTGG ACTCTTTAGC AGAGTTTTTT GAAGACCTTG CAGACAAGCC ATACACGTTT
GAGGACTATG ATGTCTCCTT TGGGAGTGGT GTCTTAACTG TCAAACTGGG TGGAGATCTA
GGAACCTATG TGATCAACAA GCAGACGCCA AACAAGCAAA TCTGGCTATC TTCTCCATCC
AGTGGACCTA AGCGTTATGA CTGGACTGGG AAAAACTGGG TGTACTCCCA CGACGGCGTG
TCCCTCCATG AGCTGCTGGC CGCAGAGCTC ACTAAAGCCT TAAAAACCAA ACTGGACTTG
TCTTCCTTGG CCTATTCCGG AAAAGATGCT TGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID |
OHu22634 |
| Clone ID Related Accession (Same CDS sequence) |
NM_181425.3
, NM_181425.2
|
| Accession Version |
NM_181425.3 Latest version! |
Documents for ORF clone product in default vector |
| Sequence Information |
ORF Nucleotide Sequence (Length: 591bp)
Protein sequence
SNP |
| Vector |
pcDNA3.1+/C-(K)DYK or customized vector |
User Manual |
| Clone information |
Clone Map |
MSDS |
| Tag on pcDNA3.1+/C-(K)DYK |
C terminal DYKDDDDK tags |
| ORF Insert Method |
CloneEZ™ Seamless cloning technology |
| Insert Structure |
linear |
| Update Date |
2019-11-10 |
| Organism |
Homo sapiens(human) |
| Product |
frataxin, mitochondrial isoform 2 preproprotein |
| Comment |
Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL162730.26, BF058880.1, BC023633.2 and AA232366.1. On May 31, 2019 this sequence version replaced NM_181425.2.
Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, compared to variant 1, that results in a frameshift. It encodes isoform 2, which has a shorter and distinct C-terminus, compared to isoform 1.
Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.
##Evidence-Data-START##
Transcript exon combination :: SRR1803617.270376.1, SRR1163657.196832.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348]
##Evidence-Data-END##
##RefSeq-Attributes-START##
gene product(s) localized to mito. :: reported by MitoCarta
##RefSeq-Attributes-END##
COMPLETENESS: complete on the 3' end.
|
1
61
121
181
241
301
361
421
481
541
| ATGTGGACTC TCGGGCGCCG CGCAGTAGCC GGCCTCCTGG CGTCACCCAG CCCAGCCCAG
GCCCAGACCC TCACCCGGGT CCCGCGGCCG GCAGAGTTGG CCCCACTCTG CGGCCGCCGT
GGCCTGCGCA CCGACATCGA TGCGACCTGC ACGCCCCGCC GCGCAAGTTC GAACCAACGT
GGCCTCAACC AGATTTGGAA TGTCAAAAAG CAGAGTGTCT ATTTGATGAA TTTGAGGAAA
TCTGGAACTT TGGGCCACCC AGGCTCTCTA GATGAGACCA CCTATGAAAG ACTAGCAGAG
GAAACGCTGG ACTCTTTAGC AGAGTTTTTT GAAGACCTTG CAGACAAGCC ATACACGTTT
GAGGACTATG ATGTCTCCTT TGGGAGTGGT GTCTTAACTG TCAAACTGGG TGGAGATCTA
GGAACCTATG TGATCAACAA GCAGACGCCA AACAAGCAAA TCTGGCTATC TTCTCCATCC
AGGTATGTAG TGGACCTAAG CGTTATGACT GGACTGGGAA AAACTGGGTG TACTCCCACG
ACGGCGTGTC CCTCCATGAG CTGCTGGCCG CAGAGCTCAC TAAAGCCTTA A |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq |
NP_852090.1 |
| CDS | 32..622 |
| Translation | |
Target ORF information:
| RefSeq Version | NM_181425.3 |
| Organism | Homo sapiens(human) |
| Definition | Homo sapiens frataxin (FXN), transcript variant 2, mRNA. |
Target ORF information:
| Epitope | DYKDDDDK |
| Bacterial selection | AMPR |
| Mammalian selection | NeoR |
| Vector | pcDNA3.1+/C-(K)DYK |
|
NM_181425.3 |
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
| ATGTGGACTC TCGGGCGCCG CGCAGTAGCC GGCCTCCTGG CGTCACCCAG CCCAGCCCAG
GCCCAGACCC TCACCCGGGT CCCGCGGCCG GCAGAGTTGG CCCCACTCTG CGGCCGCCGT
GGCCTGCGCA CCGACATCGA TGCGACCTGC ACGCCCCGCC GCGCAAGTTC GAACCAACGT
GGCCTCAACC AGATTTGGAA TGTCAAAAAG CAGAGTGTCT ATTTGATGAA TTTGAGGAAA
TCTGGAACTT TGGGCCACCC AGGCTCTCTA GATGAGACCA CCTATGAAAG ACTAGCAGAG
GAAACGCTGG ACTCTTTAGC AGAGTTTTTT GAAGACCTTG CAGACAAGCC ATACACGTTT
GAGGACTATG ATGTCTCCTT TGGGAGTGGT GTCTTAACTG TCAAACTGGG TGGAGATCTA
GGAACCTATG TGATCAACAA GCAGACGCCA AACAAGCAAA TCTGGCTATC TTCTCCATCC
AGGTATGTAG TGGACCTAAG CGTTATGACT GGACTGGGAA AAACTGGGTG TACTCCCACG
ACGGCGTGTC CCTCCATGAG CTGCTGGCCG CAGAGCTCAC TAAAGCCTTA A |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID |
OHu22634 |
| Clone ID Related Accession (Same CDS sequence) |
NM_181425.3
, NM_181425.2
|
| Accession Version |
NM_181425.2 |
Documents for ORF clone product in default vector |
| Sequence Information |
ORF Nucleotide Sequence (Length: 591bp)
Protein sequence
SNP |
| Vector |
pcDNA3.1+/C-(K)DYK or customized vector |
User Manual |
| Clone information |
Clone Map |
MSDS |
| Tag on pcDNA3.1+/C-(K)DYK |
C terminal DYKDDDDK tags |
| ORF Insert Method |
CloneEZ™ Seamless cloning technology |
| Insert Structure |
linear |
| Update Date |
2019-02-21 |
| Organism |
Homo sapiens(human) |
| Product |
frataxin, mitochondrial isoform 2 preproprotein |
| Comment |
Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL162730.26, BF058880.1, BC023633.2 and AA232366.1. On Jun 11, 2009 this sequence version replaced NM_181425.1.
Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, compared to variant 1, that results in a frameshift. It encodes isoform 2, which has a shorter and distinct C-terminus, compared to isoform 1.
Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.
##Evidence-Data-START##
Transcript exon combination :: SRR1803617.270376.1, SRR1163657.196832.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348]
##Evidence-Data-END##
##RefSeq-Attributes-START##
gene product(s) localized to mito. :: reported by MitoCarta
##RefSeq-Attributes-END##
COMPLETENESS: complete on the 3' end.
|
1
61
121
181
241
301
361
421
481
541
| ATGTGGACTC TCGGGCGCCG CGCAGTAGCC GGCCTCCTGG CGTCACCCAG CCCAGCCCAG
GCCCAGACCC TCACCCGGGT CCCGCGGCCG GCAGAGTTGG CCCCACTCTG CGGCCGCCGT
GGCCTGCGCA CCGACATCGA TGCGACCTGC ACGCCCCGCC GCGCAAGTTC GAACCAACGT
GGCCTCAACC AGATTTGGAA TGTCAAAAAG CAGAGTGTCT ATTTGATGAA TTTGAGGAAA
TCTGGAACTT TGGGCCACCC AGGCTCTCTA GATGAGACCA CCTATGAAAG ACTAGCAGAG
GAAACGCTGG ACTCTTTAGC AGAGTTTTTT GAAGACCTTG CAGACAAGCC ATACACGTTT
GAGGACTATG ATGTCTCCTT TGGGAGTGGT GTCTTAACTG TCAAACTGGG TGGAGATCTA
GGAACCTATG TGATCAACAA GCAGACGCCA AACAAGCAAA TCTGGCTATC TTCTCCATCC
AGGTATGTAG TGGACCTAAG CGTTATGACT GGACTGGGAA AAACTGGGTG TACTCCCACG
ACGGCGTGTC CCTCCATGAG CTGCTGGCCG CAGAGCTCAC TAAAGCCTTA A |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq |
NP_852090.1 |
| CDS | 221..811 |
| Translation | |
Target ORF information:
| RefSeq Version | NM_181425.2 |
| Organism | Homo sapiens(human) |
| Definition | Homo sapiens frataxin (FXN), transcript variant 2, mRNA. |
Target ORF information:
| Epitope | DYKDDDDK |
| Bacterial selection | AMPR |
| Mammalian selection | NeoR |
| Vector | pcDNA3.1+/C-(K)DYK |
|
NM_181425.2 |
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
| ATGTGGACTC TCGGGCGCCG CGCAGTAGCC GGCCTCCTGG CGTCACCCAG CCCAGCCCAG
GCCCAGACCC TCACCCGGGT CCCGCGGCCG GCAGAGTTGG CCCCACTCTG CGGCCGCCGT
GGCCTGCGCA CCGACATCGA TGCGACCTGC ACGCCCCGCC GCGCAAGTTC GAACCAACGT
GGCCTCAACC AGATTTGGAA TGTCAAAAAG CAGAGTGTCT ATTTGATGAA TTTGAGGAAA
TCTGGAACTT TGGGCCACCC AGGCTCTCTA GATGAGACCA CCTATGAAAG ACTAGCAGAG
GAAACGCTGG ACTCTTTAGC AGAGTTTTTT GAAGACCTTG CAGACAAGCC ATACACGTTT
GAGGACTATG ATGTCTCCTT TGGGAGTGGT GTCTTAACTG TCAAACTGGG TGGAGATCTA
GGAACCTATG TGATCAACAA GCAGACGCCA AACAAGCAAA TCTGGCTATC TTCTCCATCC
AGGTATGTAG TGGACCTAAG CGTTATGACT GGACTGGGAA AAACTGGGTG TACTCCCACG
ACGGCGTGTC CCTCCATGAG CTGCTGGCCG CAGAGCTCAC TAAAGCCTTA A |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID |
OHu22696 |
| Clone ID Related Accession (Same CDS sequence) |
NM_001161706.1
|
| Accession Version |
NM_001161706.1 Latest version! |
Documents for ORF clone product in default vector |
| Sequence Information |
ORF Nucleotide Sequence (Length: 516bp)
Protein sequence
SNP |
| Vector |
pcDNA3.1+/C-(K)DYK or customized vector |
User Manual |
| Clone information |
Clone Map |
MSDS |
| Tag on pcDNA3.1+/C-(K)DYK |
C terminal DYKDDDDK tags |
| ORF Insert Method |
CloneEZ™ Seamless cloning technology |
| Insert Structure |
linear |
| Update Date |
2016-07-08 |
| Organism |
Homo sapiens(Human) |
| Product |
frataxin, mitochondrial isoform 3 preproprotein |
| Comment |
REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL162730.26.
Transcript Variant: Transcript Variant: This variant (3) uses an alternate exon in the 3' coding region, compared to variant 1, that results in a frameshift. It encodes isoform 3, which has a shorter and distinct C-terminus, compared to isoform 1.
Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.
##Evidence-Data-START##
RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348]
##Evidence-Data-END##
##RefSeq-Attributes-START##
gene product(s) localized to mito. :: reported by MitoCarta
##RefSeq-Attributes-END##
COMPLETENESS: complete on the 3' end.
|
1
61
121
181
241
301
361
421
481
| ATGTGGACTC TCGGGCGCCG CGCAGTAGCC GGCCTCCTGG CGTCACCCAG CCCAGCCCAG
GCCCAGACCC TCACCCGGGT CCCGCGGCCG GCAGAGTTGG CCCCACTCTG CGGCCGCCGT
GGCCTGCGCA CCGACATCGA TGCGACCTGC ACGCCCCGCC GCGCAAGTTC GAACCAACGT
GGCCTCAACC AGATTTGGAA TGTCAAAAAG CAGAGTGTCT ATTTGATGAA TTTGAGGAAA
TCTGGAACTT TGGGCCACCC AGGCTCTCTA GATGAGACCA CCTATGAAAG ACTAGCAGAG
GAAACGCTGG ACTCTTTAGC AGAGTTTTTT GAAGACCTTG CAGACAAGCC ATACACGTTT
GAGGACTATG ATGTCTCCTT TGGGAGTGGT GTCTTAACTG TCAAACTGGG TGGAGATCTA
GGAACCTATG TGATCAACAA GCAGACGCCA AACAAGCAAA TCTGGCTATC TTCTCCATCC
AGGTTAACGT GGCTCCTGTG GCTGTTCCAT CCCTGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq |
NP_001155178.1 |
| CDS | 221..736 |
| Translation | |
Target ORF information:
| RefSeq Version | NM_001161706.1 |
| Organism | Homo sapiens(Human) |
| Definition | Homo sapiens frataxin (FXN), transcript variant 3, mRNA. |
Target ORF information:
| Epitope | DYKDDDDK |
| Bacterial selection | AMPR |
| Mammalian selection | NeoR |
| Vector | pcDNA3.1+/C-(K)DYK |
|
NM_001161706.1 |
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
| ATGTGGACTC TCGGGCGCCG CGCAGTAGCC GGCCTCCTGG CGTCACCCAG CCCAGCCCAG
GCCCAGACCC TCACCCGGGT CCCGCGGCCG GCAGAGTTGG CCCCACTCTG CGGCCGCCGT
GGCCTGCGCA CCGACATCGA TGCGACCTGC ACGCCCCGCC GCGCAAGTTC GAACCAACGT
GGCCTCAACC AGATTTGGAA TGTCAAAAAG CAGAGTGTCT ATTTGATGAA TTTGAGGAAA
TCTGGAACTT TGGGCCACCC AGGCTCTCTA GATGAGACCA CCTATGAAAG ACTAGCAGAG
GAAACGCTGG ACTCTTTAGC AGAGTTTTTT GAAGACCTTG CAGACAAGCC ATACACGTTT
GAGGACTATG ATGTCTCCTT TGGGAGTGGT GTCTTAACTG TCAAACTGGG TGGAGATCTA
GGAACCTATG TGATCAACAA GCAGACGCCA AACAAGCAAA TCTGGCTATC TTCTCCATCC
AGGTTAACGT GGCTCCTGTG GCTGTTCCAT CCCTGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
