Eif2ak4 cDNA ORF clone, Mus musculus(house mouse)

  • Gene

  • Clones

  • gRNAs

The following Eif2ak4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eif2ak4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu30529 XM_006499627.4
Latest version!
Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu17792 XM_006499625.4
Latest version!
Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu30528 XM_006499626.4
Latest version!
Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu80270 XM_017318909.2
Latest version!
Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu17698 NM_013719.3
Latest version!
Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu17792 NM_001177806.1
Latest version!
Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu30530 NM_001355383.1
Latest version!
Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OMu30530 XM_017318915.1
Latest version!
Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
View Old Accession Versions >>
OMu80270 XM_017318909.1 Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu17792 XM_006499625.3 Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu30529 XM_006499627.3 Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OMu30528 XM_006499626.3 Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu30529
    Clone ID Related Accession (Same CDS sequence) XM_006499627.4 , XM_006499627.3
    Accession Version XM_006499627.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4497bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product eIF-2-alpha kinase GCN2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006499627.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCTGGGG GCCGCGGGGC CTCTGGACGC GGCCGGGCTG AGCCACAGGA GAGCTATTCG 
    CAGCGACAGG ACCACGAGCT GCAGGCCCTG GAGGCCATTT ACGGCTCGGA CTTCCAGGAT
    CTGCGGCCGG ATGCGCGCGG ACGGGAACGT TTGGAAATCA CTAGCTTGAC AAACCAGGAC
    TATGCCTCTA AGAGAGACCC AGCAGGGCAC AGGGCAGCTG CCATCCTCCA TGGAGGCTCT
    CCTGACTTTG TAGGAAATGG TAAAGCCCGG ACATACTCCT CAGGAAGGTC CAGGCGAGAA
    CGTCAGTATT CTGTGTGTAG TGGTGAACCC TCTCCTGGCT CGTGTGACAT CCTCCATTTC
    TCTGTGGGTA GCCCTGATCA GCTCATGGTG CACAAAGGGA GATGTGTTGG CAGTGATGAG
    CAGCTGGGAA AGGTGGTATA CAATGCTTTG GAAACAGCCA CGGGCAGCTT TGTCTTGCTG
    CATGAGTGGG TCCTTCAATG GCAGAAGATG GGCCCTTGCC TTACCAGTCA AGAGAAAGAG
    AAGATTGACA AGTGCAAAAG GCAGATTCAA GGAGCAGAAA CAGAATTCAG CTCCCTAGTG
    AAACTGAGCC ATCCAAATAT CGTCCGCTAC TTTGCGATGA ACTCCAGAGA GGAGGAGGAC
    TCCATTGTGA TCGACATTCT GGCAGAGCAC GTCAGTGGCA TCTCTCTGGC TACACACCTG
    AGCCACTCGG GCCCAGTCCC CGCCCACCAG CTGCGCAAGT ACACAGCCCA GCTCCTGGCA
    GGCCTGGATT ACCTACACAG CAACTCCGTG GTGCACAAGG TTCTGAGCGC GTCCAGTGTC
    TTGGTAGATG CCGAGGGCAC TGTCAAGATA ACAGACTACA GCATCTCTAA GCGTCTGGCA
    GACATTTGCA AGGAGGATGT ATTTGAGCAA GCTCGAGTTC GTTTTAGTGA CAGTGCCCTG
    CCTTATAAAA CAGGAAAGAA AGGGGATGTG TGGCGGCTCG GCCTCCTGCT GTTGTCTCTC
    AGCCAAGGAC AGGAGTGTGG GGAGTATCCG GTGACCATCC CCAGTGACCT GCCAGCTGAC
    TTCCAAGACT TCCTGAAGAA GTGTGTCTGC CTGGACGACA AGGAAAGATG GAGCCCTCAG
    CAGCTGCTGA AACACAGCTT TATAAACCCT CAACCAAAGC TGCCTTTGGT GGAGCAGAGT
    CCTGAAGACT CTGGGGGACA AGACTACATT GAGACCGTCA TTCCCAGCAA CCAGCTGCCC
    AGCGCTGCGT TCTTCAGTGA GACACAGAAA CAGTTCTCCC GGTACTTCAT TGAGTTTGAA
    GAACTACAAC TCCTAGGGAA AGGAGCTTTT GGAGCTGTCA TCAAGGTGCA AAACAAGCTT
    GATGGCTGCT GCTATGCTGT GAAGCGCATC CCTATCAACC CTGCAAGCAG ACACTTCCGC
    CGGATCAAGG GCGAGGTGAC ACTGCTATCG CGCCTGCACC ATGAGAACAT TGTGCGCTAC
    TACAACGCCT GGATCGAGCG GCATGAGCGC CCAGCGGTGC CAGGGACACC GCCCCCAGAC
    TGCACACCCC AGGCCCAGGA CAGCCCAGCC ACCTGCGGGA AAACATCAGG CGACACTGAA
    GAACTGGGCA GCGTGGAGGC TGCAGCACCG CCACCCATCC TCAGCAGCTC GGTGGAGTGG
    AGCACATCTG CAGAGCGTTC TACCAGCACC CGCTTCCCAG TCACGGGCCA GGATTCCAGC
    AGCGATGAGG AGGACGAGGA CGAGCGCGAT GGCGTCTTCT CCCAGTCCTT TCTACCTGCT
    TCAGATTCTG ACAGTGACAT CATCTTTGAC AATGAAGATG AAAACAGTAA AAGTCAAAAT
    CAGGATGAAG ACTGCAATCA AAAGGACGGT AGCCATGAGA TTGAGCCTTC AGTGACAGCT
    GAGGCTGTGC ACTACCTTTA TATCCAGATG GAGTACTGCG AGAAGAGCAC GCTGCGGGAC
    ACCATTGACC AGGGGCTGTT CCGAGACACC AGCCGGCTCT GGAGGCTTTT CCGAGAGATT
    CTGGATGGAT TAGCTTATAT CCATGAGAAA GGAATGATTC ATCGCGACTT GAAGCCTGTC
    AATATTTTTT TGGATTCTGA TGACCATGTG AAAATAGGTG ACTTTGGCCT GGCAACAGAC
    CATCTGGCCT TCACTGCTGA AGGTAAACAG GACGACCAGG CAGGTGACGG AGTGATTAAG
    TCAGACCCTT CAGGCCATTT GACTGGCATG GTTGGTACTG CTCTGTATGT AAGCCCTGAG
    GTCCAAGGAA GCACCAAGTC TGCATACAAC CAGAAAGTGG ATCTCTTCAG CCTGGGAATT
    ATCTTCTTTG AGATGTCTTA TCACCCGATG GTGACTGCCT CAGAAAGAAT TTTCGTTCTC
    AACCAACTTC GAGATCCCAC ATCGCCCAAG TTTCCAGACG ACTTCGATGA TGGAGAACAT
    ACAAAGCAGA AATCTGTCAT CTCCTGGCTG TTGAACCATG ATCCAGCTAA ACGGCCCACG
    GCCATGGAAC TGCTCAAGAG TGAGCTGCTC CCCCCGCCGC AGATGGAGGA GTCCGAGCTG
    CATGAAGTGC TGCACCACAC GCTGGCCAAC ATAGATGGGA AGGCCTACCG CACCATGATG
    AGTCAGATCT TCTGCCAGCA CATCTCCCCC GCTATAGACT ACACCTACGA CAGTGACATC
    CTGAAGGGCA ACTTCTTGAT TCGTACAGCC AAGATCCAGC AGCTTGTATG TGAAACCATC
    GTCCGCGTCT TTAAAAGGCA TGGCGCTGTC CAGCTGTGCA CCCCACTGCT GCTTCCCCGA
    AACAGGCAAA TATATGAGCA CAACGAAGCG GCTTTGTTCA TGGATCACAG CGGCATGCTG
    GTGATGCTTC CGTTCGACCT GCGGGTCCCT TTTGCAAGAT ATGTGGCAAG AAATAACATA
    CTGAATTTAA AACGATACTG CATAGAGCGG GTGTTCAGAC CTCGGAAGTT AGACCGATTT
    CATCCCAAAG AACTCCTGGA ATGTGCATTT GATATCGTCA CTTCTACCAC CAACAGCTCT
    CTGCCCACTG CCGAAACCAT CTACACCATC TATGAAATAA TCCAGGAGTT TCCTGCACTT
    CAGGAAAGGA ATTACAGCAT TTACTTGAAC CATACGATGC TTCTGAAGGC AATACTCCTA
    CACTGTGGGA TCCCAGAGGA CAAGCTCAGC CAAGTCTACG TCATTCTGTA TGATGCTGTG
    ACAGAGAAGC TGACTAGGAG AGAAGTGGAA GCTAAATTCT GTAACCTGTC CTTGTCTTCT
    AATAGCCTGT GTAGACTCTA CAAGTTCATT GAGCAGAAGG GGGATTTGCA AGACTTAACG
    CCAACCATCA ACTCACTAAT AAAACAGAAA ACAGGCGTTG CTCAGTTGGT GAAGTATAGC
    TTAAAAGACC TCGAGGACGT CGTCGGGCTG CTGAAGAAAC TTGGCGTGAA GTTACAGGTC
    TCCATCAACC TGGGCTTGGT CTACAAGGTG CAGCAGCACA CTGGCATCAT CTTCCAGTTC
    CTGGCATTCA GCAAACGCAG GCAAAGGGTT GTGCCTGAGA TCCTTGCCGC CGGTGGCAGA
    TACGACCTGC TGATTCCCAA GTTCAGAGGC CCACAGACTG TGGGGCCAGT CCCCACTGCT
    GTCGGTGTCA GCATAGCCAT AGACAAGATA TTTGCTGCTG TCCTCAACAT GGAAGAGCCT
    GTTACAGTGA GCTCCTGTGA CCTCCTGGTT GTCAGTGTTG GCCAGATGTC CATGTCCAGG
    GCCATCAACC TAACCCAGAA ACTGTGGACA GCGGGCATCA CTGCAGAAAT CATGTATGAC
    TGGTCCCAGT CCCAGGAAGA GTTACAAGAG TACTGCAGAC ACCATGAAAT CACCTATGTG
    GCTCTGGTCT CCGATAAAGA AGGAAGCCAT GTCAAGGTCA AGTCCTTTGA GAAGGAGAGG
    CAAACAGAAA AGCGTGTATT GGAATCGGAT CTTGTGGATC ACGTTATGCA GAAACTAAGA
    ACCAAAGTTG GTGACGAAAG AAATTTCAGA GACGCTTCTG ATAATCTTGC CGTACAAACT
    CTGAAGGGGT CGTTTTCTAA TGCTTCAGGT TTGTTTGAAA TCCATGGAAC TACAGTAGTC
    CCCAACGTGA TTGTTCTAGC ACCAGAAAAG CTGTCAGCCA GCACCAGGAG GCGACATGAG
    ATTCAGGTGC AAACCCGACT TCAGACTACA CTTGCCAACT TACATCAGAA AAGCAGTGAA
    ATTGAAATTT TGGCTGTGGA CCTACCCAAG GAAACAATCT TACAGTTTCT ATCATTAGAG
    TGGGATGCTG ATGAACAGGC ATTTAACACA ACTGTGAAGC AGCTGCTGTC ACGCCTGCCA
    AAGCAAAGAT ACCTCAAACT CGTCTGCGAT GAAATTTATA ACATCAAAGT TGAAAAGAAG
    GTGTCAGTGC TGTTCCTGTA CAGCTACAGA GATGACTACT ACAGAATCCT ATTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006499690.1
    CDS126..4622
    Translation

    Target ORF information:

    RefSeq Version XM_006499627.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006499627.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCTGGGG GCCGCGGGGC CTCTGGACGC GGCCGGGCTG AGCCACAGGA GAGCTATTCG 
    CAGCGACAGG ACCACGAGCT GCAGGCCCTG GAGGCCATTT ACGGCTCGGA CTTCCAGGAT
    CTGCGGCCGG ATGCGCGCGG ACGGGAACGT TTGGAAATCA CTAGCTTGAC AAACCAGGAC
    TATGCCTCTA AGAGAGACCC AGCAGGGCAC AGGGCAGCTG CCATCCTCCA TGGAGGCTCT
    CCTGACTTTG TAGGAAATGG TAAAGCCCGG ACATACTCCT CAGGAAGGTC CAGGCGAGAA
    CGTCAGTATT CTGTGTGTAG TGGTGAACCC TCTCCTGGCT CGTGTGACAT CCTCCATTTC
    TCTGTGGGTA GCCCTGATCA GCTCATGGTG CACAAAGGGA GATGTGTTGG CAGTGATGAG
    CAGCTGGGAA AGGTGGTATA CAATGCTTTG GAAACAGCCA CGGGCAGCTT TGTCTTGCTG
    CATGAGTGGG TCCTTCAATG GCAGAAGATG GGCCCTTGCC TTACCAGTCA AGAGAAAGAG
    AAGATTGACA AGTGCAAAAG GCAGATTCAA GGAGCAGAAA CAGAATTCAG CTCCCTAGTG
    AAACTGAGCC ATCCAAATAT CGTCCGCTAC TTTGCGATGA ACTCCAGAGA GGAGGAGGAC
    TCCATTGTGA TCGACATTCT GGCAGAGCAC GTCAGTGGCA TCTCTCTGGC TACACACCTG
    AGCCACTCGG GCCCAGTCCC CGCCCACCAG CTGCGCAAGT ACACAGCCCA GCTCCTGGCA
    GGCCTGGATT ACCTACACAG CAACTCCGTG GTGCACAAGG TTCTGAGCGC GTCCAGTGTC
    TTGGTAGATG CCGAGGGCAC TGTCAAGATA ACAGACTACA GCATCTCTAA GCGTCTGGCA
    GACATTTGCA AGGAGGATGT ATTTGAGCAA GCTCGAGTTC GTTTTAGTGA CAGTGCCCTG
    CCTTATAAAA CAGGAAAGAA AGGGGATGTG TGGCGGCTCG GCCTCCTGCT GTTGTCTCTC
    AGCCAAGGAC AGGAGTGTGG GGAGTATCCG GTGACCATCC CCAGTGACCT GCCAGCTGAC
    TTCCAAGACT TCCTGAAGAA GTGTGTCTGC CTGGACGACA AGGAAAGATG GAGCCCTCAG
    CAGCTGCTGA AACACAGCTT TATAAACCCT CAACCAAAGC TGCCTTTGGT GGAGCAGAGT
    CCTGAAGACT CTGGGGGACA AGACTACATT GAGACCGTCA TTCCCAGCAA CCAGCTGCCC
    AGCGCTGCGT TCTTCAGTGA GACACAGAAA CAGTTCTCCC GGTACTTCAT TGAGTTTGAA
    GAACTACAAC TCCTAGGGAA AGGAGCTTTT GGAGCTGTCA TCAAGGTGCA AAACAAGCTT
    GATGGCTGCT GCTATGCTGT GAAGCGCATC CCTATCAACC CTGCAAGCAG ACACTTCCGC
    CGGATCAAGG GCGAGGTGAC ACTGCTATCG CGCCTGCACC ATGAGAACAT TGTGCGCTAC
    TACAACGCCT GGATCGAGCG GCATGAGCGC CCAGCGGTGC CAGGGACACC GCCCCCAGAC
    TGCACACCCC AGGCCCAGGA CAGCCCAGCC ACCTGCGGGA AAACATCAGG CGACACTGAA
    GAACTGGGCA GCGTGGAGGC TGCAGCACCG CCACCCATCC TCAGCAGCTC GGTGGAGTGG
    AGCACATCTG CAGAGCGTTC TACCAGCACC CGCTTCCCAG TCACGGGCCA GGATTCCAGC
    AGCGATGAGG AGGACGAGGA CGAGCGCGAT GGCGTCTTCT CCCAGTCCTT TCTACCTGCT
    TCAGATTCTG ACAGTGACAT CATCTTTGAC AATGAAGATG AAAACAGTAA AAGTCAAAAT
    CAGGATGAAG ACTGCAATCA AAAGGACGGT AGCCATGAGA TTGAGCCTTC AGTGACAGCT
    GAGGCTGTGC ACTACCTTTA TATCCAGATG GAGTACTGCG AGAAGAGCAC GCTGCGGGAC
    ACCATTGACC AGGGGCTGTT CCGAGACACC AGCCGGCTCT GGAGGCTTTT CCGAGAGATT
    CTGGATGGAT TAGCTTATAT CCATGAGAAA GGAATGATTC ATCGCGACTT GAAGCCTGTC
    AATATTTTTT TGGATTCTGA TGACCATGTG AAAATAGGTG ACTTTGGCCT GGCAACAGAC
    CATCTGGCCT TCACTGCTGA AGGTAAACAG GACGACCAGG CAGGTGACGG AGTGATTAAG
    TCAGACCCTT CAGGCCATTT GACTGGCATG GTTGGTACTG CTCTGTATGT AAGCCCTGAG
    GTCCAAGGAA GCACCAAGTC TGCATACAAC CAGAAAGTGG ATCTCTTCAG CCTGGGAATT
    ATCTTCTTTG AGATGTCTTA TCACCCGATG GTGACTGCCT CAGAAAGAAT TTTCGTTCTC
    AACCAACTTC GAGATCCCAC ATCGCCCAAG TTTCCAGACG ACTTCGATGA TGGAGAACAT
    ACAAAGCAGA AATCTGTCAT CTCCTGGCTG TTGAACCATG ATCCAGCTAA ACGGCCCACG
    GCCATGGAAC TGCTCAAGAG TGAGCTGCTC CCCCCGCCGC AGATGGAGGA GTCCGAGCTG
    CATGAAGTGC TGCACCACAC GCTGGCCAAC ATAGATGGGA AGGCCTACCG CACCATGATG
    AGTCAGATCT TCTGCCAGCA CATCTCCCCC GCTATAGACT ACACCTACGA CAGTGACATC
    CTGAAGGGCA ACTTCTTGAT TCGTACAGCC AAGATCCAGC AGCTTGTATG TGAAACCATC
    GTCCGCGTCT TTAAAAGGCA TGGCGCTGTC CAGCTGTGCA CCCCACTGCT GCTTCCCCGA
    AACAGGCAAA TATATGAGCA CAACGAAGCG GCTTTGTTCA TGGATCACAG CGGCATGCTG
    GTGATGCTTC CGTTCGACCT GCGGGTCCCT TTTGCAAGAT ATGTGGCAAG AAATAACATA
    CTGAATTTAA AACGATACTG CATAGAGCGG GTGTTCAGAC CTCGGAAGTT AGACCGATTT
    CATCCCAAAG AACTCCTGGA ATGTGCATTT GATATCGTCA CTTCTACCAC CAACAGCTCT
    CTGCCCACTG CCGAAACCAT CTACACCATC TATGAAATAA TCCAGGAGTT TCCTGCACTT
    CAGGAAAGGA ATTACAGCAT TTACTTGAAC CATACGATGC TTCTGAAGGC AATACTCCTA
    CACTGTGGGA TCCCAGAGGA CAAGCTCAGC CAAGTCTACG TCATTCTGTA TGATGCTGTG
    ACAGAGAAGC TGACTAGGAG AGAAGTGGAA GCTAAATTCT GTAACCTGTC CTTGTCTTCT
    AATAGCCTGT GTAGACTCTA CAAGTTCATT GAGCAGAAGG GGGATTTGCA AGACTTAACG
    CCAACCATCA ACTCACTAAT AAAACAGAAA ACAGGCGTTG CTCAGTTGGT GAAGTATAGC
    TTAAAAGACC TCGAGGACGT CGTCGGGCTG CTGAAGAAAC TTGGCGTGAA GTTACAGGTC
    TCCATCAACC TGGGCTTGGT CTACAAGGTG CAGCAGCACA CTGGCATCAT CTTCCAGTTC
    CTGGCATTCA GCAAACGCAG GCAAAGGGTT GTGCCTGAGA TCCTTGCCGC CGGTGGCAGA
    TACGACCTGC TGATTCCCAA GTTCAGAGGC CCACAGACTG TGGGGCCAGT CCCCACTGCT
    GTCGGTGTCA GCATAGCCAT AGACAAGATA TTTGCTGCTG TCCTCAACAT GGAAGAGCCT
    GTTACAGTGA GCTCCTGTGA CCTCCTGGTT GTCAGTGTTG GCCAGATGTC CATGTCCAGG
    GCCATCAACC TAACCCAGAA ACTGTGGACA GCGGGCATCA CTGCAGAAAT CATGTATGAC
    TGGTCCCAGT CCCAGGAAGA GTTACAAGAG TACTGCAGAC ACCATGAAAT CACCTATGTG
    GCTCTGGTCT CCGATAAAGA AGGAAGCCAT GTCAAGGTCA AGTCCTTTGA GAAGGAGAGG
    CAAACAGAAA AGCGTGTATT GGAATCGGAT CTTGTGGATC ACGTTATGCA GAAACTAAGA
    ACCAAAGTTG GTGACGAAAG AAATTTCAGA GACGCTTCTG ATAATCTTGC CGTACAAACT
    CTGAAGGGGT CGTTTTCTAA TGCTTCAGGT TTGTTTGAAA TCCATGGAAC TACAGTAGTC
    CCCAACGTGA TTGTTCTAGC ACCAGAAAAG CTGTCAGCCA GCACCAGGAG GCGACATGAG
    ATTCAGGTGC AAACCCGACT TCAGACTACA CTTGCCAACT TACATCAGAA AAGCAGTGAA
    ATTGAAATTT TGGCTGTGGA CCTACCCAAG GAAACAATCT TACAGTTTCT ATCATTAGAG
    TGGGATGCTG ATGAACAGGC ATTTAACACA ACTGTGAAGC AGCTGCTGTC ACGCCTGCCA
    AAGCAAAGAT ACCTCAAACT CGTCTGCGAT GAAATTTATA ACATCAAAGT TGAAAAGAAG
    GTGTCAGTGC TGTTCCTGTA CAGCTACAGA GATGACTACT ACAGAATCCT ATTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu17792
    Clone ID Related Accession (Same CDS sequence) NM_001177806.1 , XM_006499625.3 , XM_006499625.4
    Accession Version XM_006499625.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4611bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product eIF-2-alpha kinase GCN2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006499625.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCCCACCT ACATACCCAG ATGTGTGATG ATATTTGAAC TGGCACACCA TGTGCAGTCG 
    TTTCTCAGCG AGCATAACAA GCCCCCACCA AAGTCTTTCC ATGAAGAAAT GCTGGAAAGG
    CAGGCTCAGG AGAAGCAGCA GAGGTTGCTG GAGGCCAGGC GGAAGGAGGA GCAGGAGCAA
    CGTGAAATCC TCCACGAGAT TCAGAGAAGG AAAGAGGAGA TCAAGGAAGA GAAGAAAAGG
    AAGGAAATGG CTAAGCAGGA ACGTTTGGAA ATCACTAGCT TGACAAACCA GGACTATGCC
    TCTAAGAGAG ACCCAGCAGG GCACAGGGCA GCTGCCATCC TCCATGGAGG CTCTCCTGAC
    TTTGTAGGAA ATGGTAAAGC CCGGACATAC TCCTCAGGAA GGTCCAGGCG AGAACGTCAG
    TATTCTGTGT GTAGTGGTGA ACCCTCTCCT GGCTCGTGTG ACATCCTCCA TTTCTCTGTG
    GGTAGCCCTG ATCAGCTCAT GGTGCACAAA GGGAGATGTG TTGGCAGTGA TGAGCAGCTG
    GGAAAGGTGG TATACAATGC TTTGGAAACA GCCACGGGCA GCTTTGTCTT GCTGCATGAG
    TGGGTCCTTC AATGGCAGAA GATGGGCCCT TGCCTTACCA GTCAAGAGAA AGAGAAGATT
    GACAAGTGCA AAAGGCAGAT TCAAGGAGCA GAAACAGAAT TCAGCTCCCT AGTGAAACTG
    AGCCATCCAA ATATCGTCCG CTACTTTGCG ATGAACTCCA GAGAGGAGGA GGACTCCATT
    GTGATCGACA TTCTGGCAGA GCACGTCAGT GGCATCTCTC TGGCTACACA CCTGAGCCAC
    TCGGGCCCAG TCCCCGCCCA CCAGCTGCGC AAGTACACAG CCCAGCTCCT GGCAGGCCTG
    GATTACCTAC ACAGCAACTC CGTGGTGCAC AAGGTTCTGA GCGCGTCCAG TGTCTTGGTA
    GATGCCGAGG GCACTGTCAA GATAACAGAC TACAGCATCT CTAAGCGTCT GGCAGACATT
    TGCAAGGAGG ATGTATTTGA GCAAGCTCGA GTTCGTTTTA GTGACAGTGC CCTGCCTTAT
    AAAACAGGAA AGAAAGGGGA TGTGTGGCGG CTCGGCCTCC TGCTGTTGTC TCTCAGCCAA
    GGACAGGAGT GTGGGGAGTA TCCGGTGACC ATCCCCAGTG ACCTGCCAGC TGACTTCCAA
    GACTTCCTGA AGAAGTGTGT CTGCCTGGAC GACAAGGAAA GATGGAGCCC TCAGCAGCTG
    CTGAAACACA GCTTTATAAA CCCTCAACCA AAGCTGCCTT TGGTGGAGCA GAGTCCTGAA
    GACTCTGGGG GACAAGACTA CATTGAGACC GTCATTCCCA GCAACCAGCT GCCCAGCGCT
    GCGTTCTTCA GTGAGACACA GAAACAGTTC TCCCGGTACT TCATTGAGTT TGAAGAACTA
    CAACTCCTAG GGAAAGGAGC TTTTGGAGCT GTCATCAAGG TGCAAAACAA GCTTGATGGC
    TGCTGCTATG CTGTGAAGCG CATCCCTATC AACCCTGCAA GCAGACACTT CCGCCGGATC
    AAGGGCGAGG TGACACTGCT ATCGCGCCTG CACCATGAGA ACATTGTGCG CTACTACAAC
    GCCTGGATCG AGCGGCATGA GCGCCCAGCG GTGCCAGGGA CACCGCCCCC AGACTGCACA
    CCCCAGGCCC AGGACAGCCC AGCCACCTGC GGGAAAACAT CAGGCGACAC TGAAGAACTG
    GGCAGCGTGG AGGCTGCAGC ACCGCCACCC ATCCTCAGCA GCTCGGTGGA GTGGAGCACA
    TCTGCAGAGC GTTCTACCAG CACCCGCTTC CCAGTCACGG GCCAGGATTC CAGCAGCGAT
    GAGGAGGACG AGGACGAGCG CGATGGCGTC TTCTCCCAGT CCTTTCTACC TGCTTCAGAT
    TCTGACAGTG ACATCATCTT TGACAATGAA GATGAAAACA GTAAAAGTCA AAATCAGGAT
    GAAGACTGCA ATCAAAAGGA CGGTAGCCAT GAGATTGAGC CTTCAGTGAC AGCTGAGGCT
    GTGCACTACC TTTATATCCA GATGGAGTAC TGCGAGAAGA GCACGCTGCG GGACACCATT
    GACCAGGGGC TGTTCCGAGA CACCAGCCGG CTCTGGAGGC TTTTCCGAGA GATTCTGGAT
    GGATTAGCTT ATATCCATGA GAAAGGAATG ATTCATCGCG ACTTGAAGCC TGTCAATATT
    TTTTTGGATT CTGATGACCA TGTGAAAATA GGTGACTTTG GCCTGGCAAC AGACCATCTG
    GCCTTCACTG CTGAAGGTAA ACAGGACGAC CAGGCAGGTG ACGGAGTGAT TAAGTCAGAC
    CCTTCAGGCC ATTTGACTGG CATGGTTGGT ACTGCTCTGT ATGTAAGCCC TGAGGTCCAA
    GGAAGCACCA AGTCTGCATA CAACCAGAAA GTGGATCTCT TCAGCCTGGG AATTATCTTC
    TTTGAGATGT CTTATCACCC GATGGTGACT GCCTCAGAAA GAATTTTCGT TCTCAACCAA
    CTTCGAGATC CCACATCGCC CAAGTTTCCA GACGACTTCG ATGATGGAGA ACATACAAAG
    CAGAAATCTG TCATCTCCTG GCTGTTGAAC CATGATCCAG CTAAACGGCC CACGGCCATG
    GAACTGCTCA AGAGTGAGCT GCTCCCCCCG CCGCAGATGG AGGAGTCCGA GCTGCATGAA
    GTGCTGCACC ACACGCTGGC CAACATAGAT GGGAAGGCCT ACCGCACCAT GATGAGTCAG
    ATCTTCTGCC AGCACATCTC CCCCGCTATA GACTACACCT ACGACAGTGA CATCCTGAAG
    GGCAACTTCT TGATTCGTAC AGCCAAGATC CAGCAGCTTG TATGTGAAAC CATCGTCCGC
    GTCTTTAAAA GGCATGGCGC TGTCCAGCTG TGCACCCCAC TGCTGCTTCC CCGAAACAGG
    CAAATATATG AGCACAACGA AGCGGCTTTG TTCATGGATC ACAGCGGCAT GCTGGTGATG
    CTTCCGTTCG ACCTGCGGGT CCCTTTTGCA AGATATGTGG CAAGAAATAA CATACTGAAT
    TTAAAACGAT ACTGCATAGA GCGGGTGTTC AGACCTCGGA AGTTAGACCG ATTTCATCCC
    AAAGAACTCC TGGAATGTGC ATTTGATATC GTCACTTCTA CCACCAACAG CTCTCTGCCC
    ACTGCCGAAA CCATCTACAC CATCTATGAA ATAATCCAGG AGTTTCCTGC ACTTCAGGAA
    AGGAATTACA GCATTTACTT GAACCATACG ATGCTTCTGA AGGCAATACT CCTACACTGT
    GGGATCCCAG AGGACAAGCT CAGCCAAGTC TACGTCATTC TGTATGATGC TGTGACAGAG
    AAGCTGACTA GGAGAGAAGT GGAAGCTAAA TTCTGTAACC TGTCCTTGTC TTCTAATAGC
    CTGTGTAGAC TCTACAAGTT CATTGAGCAG AAGGGGGATT TGCAAGACTT AACGCCAACC
    ATCAACTCAC TAATAAAACA GAAAACAGGC GTTGCTCAGT TGGTGAAGTA TAGCTTAAAA
    GACCTCGAGG ACGTCGTCGG GCTGCTGAAG AAACTTGGCG TGAAGTTACA GGTCTCCATC
    AACCTGGGCT TGGTCTACAA GGTGCAGCAG CACACTGGCA TCATCTTCCA GTTCCTGGCA
    TTCAGCAAAC GCAGGCAAAG GGTTGTGCCT GAGATCCTTG CCGCCGGTGG CAGATACGAC
    CTGCTGATTC CCAAGTTCAG AGGCCCACAG ACTGTGGGGC CAGTCCCCAC TGCTGTCGGT
    GTCAGCATAG CCATAGACAA GATATTTGCT GCTGTCCTCA ACATGGAAGA GCCTGTTACA
    GTGAGCTCCT GTGACCTCCT GGTTGTCAGT GTTGGCCAGA TGTCCATGTC CAGGGCCATC
    AACCTAACCC AGAAACTGTG GACAGCGGGC ATCACTGCAG AAATCATGTA TGACTGGTCC
    CAGTCCCAGG AAGAGTTACA AGAGTACTGC AGACACCATG AAATCACCTA TGTGGCTCTG
    GTCTCCGATA AAGAAGGAAG CCATGTCAAG GTCAAGTCCT TTGAGAAGGA GAGGCAAACA
    GAAAAGCGTG TATTGGAATC GGATCTTGTG GATCACGTTA TGCAGAAACT AAGAACCAAA
    GTTGGTGACG AAAGAAATTT CAGAGACGCT TCTGATAATC TTGCCGTACA AACTCTGAAG
    GGGTCGTTTT CTAATGCTTC AGGTTTGTTT GAAATCCATG GAACTACAGT AGTCCCCAAC
    GTGATTGTTC TAGCACCAGA AAAGCTGTCA GCCAGCACCA GGAGGCGACA TGAGATTCAG
    GTGCAAACCC GACTTCAGAC TACACTTGCC AACTTACATC AGAAAAGCAG TGAAATTGAA
    ATTTTGGCTG TGGACCTACC CAAGGAAACA ATCTTACAGT TTCTATCATT AGAGTGGGAT
    GCTGATGAAC AGGCATTTAA CACAACTGTG AAGCAGCTGC TGTCACGCCT GCCAAAGCAA
    AGATACCTCA AACTCGTCTG CGATGAAATT TATAACATCA AAGTTGAAAA GAAGGTGTCA
    GTGCTGTTCC TGTACAGCTA CAGAGATGAC TACTACAGAA TCCTATTTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006499688.1
    CDS359..4969
    Translation

    Target ORF information:

    RefSeq Version XM_006499625.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006499625.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCCCACCT ACATACCCAG ATGTGTGATG ATATTTGAAC TGGCACACCA TGTGCAGTCG 
    TTTCTCAGCG AGCATAACAA GCCCCCACCA AAGTCTTTCC ATGAAGAAAT GCTGGAAAGG
    CAGGCTCAGG AGAAGCAGCA GAGGTTGCTG GAGGCCAGGC GGAAGGAGGA GCAGGAGCAA
    CGTGAAATCC TCCACGAGAT TCAGAGAAGG AAAGAGGAGA TCAAGGAAGA GAAGAAAAGG
    AAGGAAATGG CTAAGCAGGA ACGTTTGGAA ATCACTAGCT TGACAAACCA GGACTATGCC
    TCTAAGAGAG ACCCAGCAGG GCACAGGGCA GCTGCCATCC TCCATGGAGG CTCTCCTGAC
    TTTGTAGGAA ATGGTAAAGC CCGGACATAC TCCTCAGGAA GGTCCAGGCG AGAACGTCAG
    TATTCTGTGT GTAGTGGTGA ACCCTCTCCT GGCTCGTGTG ACATCCTCCA TTTCTCTGTG
    GGTAGCCCTG ATCAGCTCAT GGTGCACAAA GGGAGATGTG TTGGCAGTGA TGAGCAGCTG
    GGAAAGGTGG TATACAATGC TTTGGAAACA GCCACGGGCA GCTTTGTCTT GCTGCATGAG
    TGGGTCCTTC AATGGCAGAA GATGGGCCCT TGCCTTACCA GTCAAGAGAA AGAGAAGATT
    GACAAGTGCA AAAGGCAGAT TCAAGGAGCA GAAACAGAAT TCAGCTCCCT AGTGAAACTG
    AGCCATCCAA ATATCGTCCG CTACTTTGCG ATGAACTCCA GAGAGGAGGA GGACTCCATT
    GTGATCGACA TTCTGGCAGA GCACGTCAGT GGCATCTCTC TGGCTACACA CCTGAGCCAC
    TCGGGCCCAG TCCCCGCCCA CCAGCTGCGC AAGTACACAG CCCAGCTCCT GGCAGGCCTG
    GATTACCTAC ACAGCAACTC CGTGGTGCAC AAGGTTCTGA GCGCGTCCAG TGTCTTGGTA
    GATGCCGAGG GCACTGTCAA GATAACAGAC TACAGCATCT CTAAGCGTCT GGCAGACATT
    TGCAAGGAGG ATGTATTTGA GCAAGCTCGA GTTCGTTTTA GTGACAGTGC CCTGCCTTAT
    AAAACAGGAA AGAAAGGGGA TGTGTGGCGG CTCGGCCTCC TGCTGTTGTC TCTCAGCCAA
    GGACAGGAGT GTGGGGAGTA TCCGGTGACC ATCCCCAGTG ACCTGCCAGC TGACTTCCAA
    GACTTCCTGA AGAAGTGTGT CTGCCTGGAC GACAAGGAAA GATGGAGCCC TCAGCAGCTG
    CTGAAACACA GCTTTATAAA CCCTCAACCA AAGCTGCCTT TGGTGGAGCA GAGTCCTGAA
    GACTCTGGGG GACAAGACTA CATTGAGACC GTCATTCCCA GCAACCAGCT GCCCAGCGCT
    GCGTTCTTCA GTGAGACACA GAAACAGTTC TCCCGGTACT TCATTGAGTT TGAAGAACTA
    CAACTCCTAG GGAAAGGAGC TTTTGGAGCT GTCATCAAGG TGCAAAACAA GCTTGATGGC
    TGCTGCTATG CTGTGAAGCG CATCCCTATC AACCCTGCAA GCAGACACTT CCGCCGGATC
    AAGGGCGAGG TGACACTGCT ATCGCGCCTG CACCATGAGA ACATTGTGCG CTACTACAAC
    GCCTGGATCG AGCGGCATGA GCGCCCAGCG GTGCCAGGGA CACCGCCCCC AGACTGCACA
    CCCCAGGCCC AGGACAGCCC AGCCACCTGC GGGAAAACAT CAGGCGACAC TGAAGAACTG
    GGCAGCGTGG AGGCTGCAGC ACCGCCACCC ATCCTCAGCA GCTCGGTGGA GTGGAGCACA
    TCTGCAGAGC GTTCTACCAG CACCCGCTTC CCAGTCACGG GCCAGGATTC CAGCAGCGAT
    GAGGAGGACG AGGACGAGCG CGATGGCGTC TTCTCCCAGT CCTTTCTACC TGCTTCAGAT
    TCTGACAGTG ACATCATCTT TGACAATGAA GATGAAAACA GTAAAAGTCA AAATCAGGAT
    GAAGACTGCA ATCAAAAGGA CGGTAGCCAT GAGATTGAGC CTTCAGTGAC AGCTGAGGCT
    GTGCACTACC TTTATATCCA GATGGAGTAC TGCGAGAAGA GCACGCTGCG GGACACCATT
    GACCAGGGGC TGTTCCGAGA CACCAGCCGG CTCTGGAGGC TTTTCCGAGA GATTCTGGAT
    GGATTAGCTT ATATCCATGA GAAAGGAATG ATTCATCGCG ACTTGAAGCC TGTCAATATT
    TTTTTGGATT CTGATGACCA TGTGAAAATA GGTGACTTTG GCCTGGCAAC AGACCATCTG
    GCCTTCACTG CTGAAGGTAA ACAGGACGAC CAGGCAGGTG ACGGAGTGAT TAAGTCAGAC
    CCTTCAGGCC ATTTGACTGG CATGGTTGGT ACTGCTCTGT ATGTAAGCCC TGAGGTCCAA
    GGAAGCACCA AGTCTGCATA CAACCAGAAA GTGGATCTCT TCAGCCTGGG AATTATCTTC
    TTTGAGATGT CTTATCACCC GATGGTGACT GCCTCAGAAA GAATTTTCGT TCTCAACCAA
    CTTCGAGATC CCACATCGCC CAAGTTTCCA GACGACTTCG ATGATGGAGA ACATACAAAG
    CAGAAATCTG TCATCTCCTG GCTGTTGAAC CATGATCCAG CTAAACGGCC CACGGCCATG
    GAACTGCTCA AGAGTGAGCT GCTCCCCCCG CCGCAGATGG AGGAGTCCGA GCTGCATGAA
    GTGCTGCACC ACACGCTGGC CAACATAGAT GGGAAGGCCT ACCGCACCAT GATGAGTCAG
    ATCTTCTGCC AGCACATCTC CCCCGCTATA GACTACACCT ACGACAGTGA CATCCTGAAG
    GGCAACTTCT TGATTCGTAC AGCCAAGATC CAGCAGCTTG TATGTGAAAC CATCGTCCGC
    GTCTTTAAAA GGCATGGCGC TGTCCAGCTG TGCACCCCAC TGCTGCTTCC CCGAAACAGG
    CAAATATATG AGCACAACGA AGCGGCTTTG TTCATGGATC ACAGCGGCAT GCTGGTGATG
    CTTCCGTTCG ACCTGCGGGT CCCTTTTGCA AGATATGTGG CAAGAAATAA CATACTGAAT
    TTAAAACGAT ACTGCATAGA GCGGGTGTTC AGACCTCGGA AGTTAGACCG ATTTCATCCC
    AAAGAACTCC TGGAATGTGC ATTTGATATC GTCACTTCTA CCACCAACAG CTCTCTGCCC
    ACTGCCGAAA CCATCTACAC CATCTATGAA ATAATCCAGG AGTTTCCTGC ACTTCAGGAA
    AGGAATTACA GCATTTACTT GAACCATACG ATGCTTCTGA AGGCAATACT CCTACACTGT
    GGGATCCCAG AGGACAAGCT CAGCCAAGTC TACGTCATTC TGTATGATGC TGTGACAGAG
    AAGCTGACTA GGAGAGAAGT GGAAGCTAAA TTCTGTAACC TGTCCTTGTC TTCTAATAGC
    CTGTGTAGAC TCTACAAGTT CATTGAGCAG AAGGGGGATT TGCAAGACTT AACGCCAACC
    ATCAACTCAC TAATAAAACA GAAAACAGGC GTTGCTCAGT TGGTGAAGTA TAGCTTAAAA
    GACCTCGAGG ACGTCGTCGG GCTGCTGAAG AAACTTGGCG TGAAGTTACA GGTCTCCATC
    AACCTGGGCT TGGTCTACAA GGTGCAGCAG CACACTGGCA TCATCTTCCA GTTCCTGGCA
    TTCAGCAAAC GCAGGCAAAG GGTTGTGCCT GAGATCCTTG CCGCCGGTGG CAGATACGAC
    CTGCTGATTC CCAAGTTCAG AGGCCCACAG ACTGTGGGGC CAGTCCCCAC TGCTGTCGGT
    GTCAGCATAG CCATAGACAA GATATTTGCT GCTGTCCTCA ACATGGAAGA GCCTGTTACA
    GTGAGCTCCT GTGACCTCCT GGTTGTCAGT GTTGGCCAGA TGTCCATGTC CAGGGCCATC
    AACCTAACCC AGAAACTGTG GACAGCGGGC ATCACTGCAG AAATCATGTA TGACTGGTCC
    CAGTCCCAGG AAGAGTTACA AGAGTACTGC AGACACCATG AAATCACCTA TGTGGCTCTG
    GTCTCCGATA AAGAAGGAAG CCATGTCAAG GTCAAGTCCT TTGAGAAGGA GAGGCAAACA
    GAAAAGCGTG TATTGGAATC GGATCTTGTG GATCACGTTA TGCAGAAACT AAGAACCAAA
    GTTGGTGACG AAAGAAATTT CAGAGACGCT TCTGATAATC TTGCCGTACA AACTCTGAAG
    GGGTCGTTTT CTAATGCTTC AGGTTTGTTT GAAATCCATG GAACTACAGT AGTCCCCAAC
    GTGATTGTTC TAGCACCAGA AAAGCTGTCA GCCAGCACCA GGAGGCGACA TGAGATTCAG
    GTGCAAACCC GACTTCAGAC TACACTTGCC AACTTACATC AGAAAAGCAG TGAAATTGAA
    ATTTTGGCTG TGGACCTACC CAAGGAAACA ATCTTACAGT TTCTATCATT AGAGTGGGAT
    GCTGATGAAC AGGCATTTAA CACAACTGTG AAGCAGCTGC TGTCACGCCT GCCAAAGCAA
    AGATACCTCA AACTCGTCTG CGATGAAATT TATAACATCA AAGTTGAAAA GAAGGTGTCA
    GTGCTGTTCC TGTACAGCTA CAGAGATGAC TACTACAGAA TCCTATTTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu30528
    Clone ID Related Accession (Same CDS sequence) XM_006499626.3 , XM_006499626.4
    Accession Version XM_006499626.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4578bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product eIF-2-alpha kinase GCN2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006499626.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGCTGGGG GCCGCGGGGC CTCTGGACGC GGCCGGGCTG AGCCACAGGA GAGCTATTCG 
    CAGCGACAGG ACCACGAGCT GCAGGCCCTG GAGGCCATTT ACGGCTCGGA CTTCCAGGAT
    CTGCGGCCGG ATGCGCGCGG ACGGCAACGT GAAATCCTCC ACGAGATTCA GAGAAGGAAA
    GAGGAGATCA AGGAAGAGAA GAAAAGGAAG GAAATGGCTA AGCAGGAACG TTTGGAAATC
    ACTAGCTTGA CAAACCAGGA CTATGCCTCT AAGAGAGACC CAGCAGGGCA CAGGGCAGCT
    GCCATCCTCC ATGGAGGCTC TCCTGACTTT GTAGGAAATG GTAAAGCCCG GACATACTCC
    TCAGGAAGGT CCAGGCGAGA ACGTCAGTAT TCTGTGTGTA GTGGTGAACC CTCTCCTGGC
    TCGTGTGACA TCCTCCATTT CTCTGTGGGT AGCCCTGATC AGCTCATGGT GCACAAAGGG
    AGATGTGTTG GCAGTGATGA GCAGCTGGGA AAGGTGGTAT ACAATGCTTT GGAAACAGCC
    ACGGGCAGCT TTGTCTTGCT GCATGAGTGG GTCCTTCAAT GGCAGAAGAT GGGCCCTTGC
    CTTACCAGTC AAGAGAAAGA GAAGATTGAC AAGTGCAAAA GGCAGATTCA AGGAGCAGAA
    ACAGAATTCA GCTCCCTAGT GAAACTGAGC CATCCAAATA TCGTCCGCTA CTTTGCGATG
    AACTCCAGAG AGGAGGAGGA CTCCATTGTG ATCGACATTC TGGCAGAGCA CGTCAGTGGC
    ATCTCTCTGG CTACACACCT GAGCCACTCG GGCCCAGTCC CCGCCCACCA GCTGCGCAAG
    TACACAGCCC AGCTCCTGGC AGGCCTGGAT TACCTACACA GCAACTCCGT GGTGCACAAG
    GTTCTGAGCG CGTCCAGTGT CTTGGTAGAT GCCGAGGGCA CTGTCAAGAT AACAGACTAC
    AGCATCTCTA AGCGTCTGGC AGACATTTGC AAGGAGGATG TATTTGAGCA AGCTCGAGTT
    CGTTTTAGTG ACAGTGCCCT GCCTTATAAA ACAGGAAAGA AAGGGGATGT GTGGCGGCTC
    GGCCTCCTGC TGTTGTCTCT CAGCCAAGGA CAGGAGTGTG GGGAGTATCC GGTGACCATC
    CCCAGTGACC TGCCAGCTGA CTTCCAAGAC TTCCTGAAGA AGTGTGTCTG CCTGGACGAC
    AAGGAAAGAT GGAGCCCTCA GCAGCTGCTG AAACACAGCT TTATAAACCC TCAACCAAAG
    CTGCCTTTGG TGGAGCAGAG TCCTGAAGAC TCTGGGGGAC AAGACTACAT TGAGACCGTC
    ATTCCCAGCA ACCAGCTGCC CAGCGCTGCG TTCTTCAGTG AGACACAGAA ACAGTTCTCC
    CGGTACTTCA TTGAGTTTGA AGAACTACAA CTCCTAGGGA AAGGAGCTTT TGGAGCTGTC
    ATCAAGGTGC AAAACAAGCT TGATGGCTGC TGCTATGCTG TGAAGCGCAT CCCTATCAAC
    CCTGCAAGCA GACACTTCCG CCGGATCAAG GGCGAGGTGA CACTGCTATC GCGCCTGCAC
    CATGAGAACA TTGTGCGCTA CTACAACGCC TGGATCGAGC GGCATGAGCG CCCAGCGGTG
    CCAGGGACAC CGCCCCCAGA CTGCACACCC CAGGCCCAGG ACAGCCCAGC CACCTGCGGG
    AAAACATCAG GCGACACTGA AGAACTGGGC AGCGTGGAGG CTGCAGCACC GCCACCCATC
    CTCAGCAGCT CGGTGGAGTG GAGCACATCT GCAGAGCGTT CTACCAGCAC CCGCTTCCCA
    GTCACGGGCC AGGATTCCAG CAGCGATGAG GAGGACGAGG ACGAGCGCGA TGGCGTCTTC
    TCCCAGTCCT TTCTACCTGC TTCAGATTCT GACAGTGACA TCATCTTTGA CAATGAAGAT
    GAAAACAGTA AAAGTCAAAA TCAGGATGAA GACTGCAATC AAAAGGACGG TAGCCATGAG
    ATTGAGCCTT CAGTGACAGC TGAGGCTGTG CACTACCTTT ATATCCAGAT GGAGTACTGC
    GAGAAGAGCA CGCTGCGGGA CACCATTGAC CAGGGGCTGT TCCGAGACAC CAGCCGGCTC
    TGGAGGCTTT TCCGAGAGAT TCTGGATGGA TTAGCTTATA TCCATGAGAA AGGAATGATT
    CATCGCGACT TGAAGCCTGT CAATATTTTT TTGGATTCTG ATGACCATGT GAAAATAGGT
    GACTTTGGCC TGGCAACAGA CCATCTGGCC TTCACTGCTG AAGGTAAACA GGACGACCAG
    GCAGGTGACG GAGTGATTAA GTCAGACCCT TCAGGCCATT TGACTGGCAT GGTTGGTACT
    GCTCTGTATG TAAGCCCTGA GGTCCAAGGA AGCACCAAGT CTGCATACAA CCAGAAAGTG
    GATCTCTTCA GCCTGGGAAT TATCTTCTTT GAGATGTCTT ATCACCCGAT GGTGACTGCC
    TCAGAAAGAA TTTTCGTTCT CAACCAACTT CGAGATCCCA CATCGCCCAA GTTTCCAGAC
    GACTTCGATG ATGGAGAACA TACAAAGCAG AAATCTGTCA TCTCCTGGCT GTTGAACCAT
    GATCCAGCTA AACGGCCCAC GGCCATGGAA CTGCTCAAGA GTGAGCTGCT CCCCCCGCCG
    CAGATGGAGG AGTCCGAGCT GCATGAAGTG CTGCACCACA CGCTGGCCAA CATAGATGGG
    AAGGCCTACC GCACCATGAT GAGTCAGATC TTCTGCCAGC ACATCTCCCC CGCTATAGAC
    TACACCTACG ACAGTGACAT CCTGAAGGGC AACTTCTTGA TTCGTACAGC CAAGATCCAG
    CAGCTTGTAT GTGAAACCAT CGTCCGCGTC TTTAAAAGGC ATGGCGCTGT CCAGCTGTGC
    ACCCCACTGC TGCTTCCCCG AAACAGGCAA ATATATGAGC ACAACGAAGC GGCTTTGTTC
    ATGGATCACA GCGGCATGCT GGTGATGCTT CCGTTCGACC TGCGGGTCCC TTTTGCAAGA
    TATGTGGCAA GAAATAACAT ACTGAATTTA AAACGATACT GCATAGAGCG GGTGTTCAGA
    CCTCGGAAGT TAGACCGATT TCATCCCAAA GAACTCCTGG AATGTGCATT TGATATCGTC
    ACTTCTACCA CCAACAGCTC TCTGCCCACT GCCGAAACCA TCTACACCAT CTATGAAATA
    ATCCAGGAGT TTCCTGCACT TCAGGAAAGG AATTACAGCA TTTACTTGAA CCATACGATG
    CTTCTGAAGG CAATACTCCT ACACTGTGGG ATCCCAGAGG ACAAGCTCAG CCAAGTCTAC
    GTCATTCTGT ATGATGCTGT GACAGAGAAG CTGACTAGGA GAGAAGTGGA AGCTAAATTC
    TGTAACCTGT CCTTGTCTTC TAATAGCCTG TGTAGACTCT ACAAGTTCAT TGAGCAGAAG
    GGGGATTTGC AAGACTTAAC GCCAACCATC AACTCACTAA TAAAACAGAA AACAGGCGTT
    GCTCAGTTGG TGAAGTATAG CTTAAAAGAC CTCGAGGACG TCGTCGGGCT GCTGAAGAAA
    CTTGGCGTGA AGTTACAGGT CTCCATCAAC CTGGGCTTGG TCTACAAGGT GCAGCAGCAC
    ACTGGCATCA TCTTCCAGTT CCTGGCATTC AGCAAACGCA GGCAAAGGGT TGTGCCTGAG
    ATCCTTGCCG CCGGTGGCAG ATACGACCTG CTGATTCCCA AGTTCAGAGG CCCACAGACT
    GTGGGGCCAG TCCCCACTGC TGTCGGTGTC AGCATAGCCA TAGACAAGAT ATTTGCTGCT
    GTCCTCAACA TGGAAGAGCC TGTTACAGTG AGCTCCTGTG ACCTCCTGGT TGTCAGTGTT
    GGCCAGATGT CCATGTCCAG GGCCATCAAC CTAACCCAGA AACTGTGGAC AGCGGGCATC
    ACTGCAGAAA TCATGTATGA CTGGTCCCAG TCCCAGGAAG AGTTACAAGA GTACTGCAGA
    CACCATGAAA TCACCTATGT GGCTCTGGTC TCCGATAAAG AAGGAAGCCA TGTCAAGGTC
    AAGTCCTTTG AGAAGGAGAG GCAAACAGAA AAGCGTGTAT TGGAATCGGA TCTTGTGGAT
    CACGTTATGC AGAAACTAAG AACCAAAGTT GGTGACGAAA GAAATTTCAG AGACGCTTCT
    GATAATCTTG CCGTACAAAC TCTGAAGGGG TCGTTTTCTA ATGCTTCAGG TTTGTTTGAA
    ATCCATGGAA CTACAGTAGT CCCCAACGTG ATTGTTCTAG CACCAGAAAA GCTGTCAGCC
    AGCACCAGGA GGCGACATGA GATTCAGGTG CAAACCCGAC TTCAGACTAC ACTTGCCAAC
    TTACATCAGA AAAGCAGTGA AATTGAAATT TTGGCTGTGG ACCTACCCAA GGAAACAATC
    TTACAGTTTC TATCATTAGA GTGGGATGCT GATGAACAGG CATTTAACAC AACTGTGAAG
    CAGCTGCTGT CACGCCTGCC AAAGCAAAGA TACCTCAAAC TCGTCTGCGA TGAAATTTAT
    AACATCAAAG TTGAAAAGAA GGTGTCAGTG CTGTTCCTGT ACAGCTACAG AGATGACTAC
    TACAGAATCC TATTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006499689.1
    CDS126..4703
    Translation

    Target ORF information:

    RefSeq Version XM_006499626.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006499626.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGCTGGGG GCCGCGGGGC CTCTGGACGC GGCCGGGCTG AGCCACAGGA GAGCTATTCG 
    CAGCGACAGG ACCACGAGCT GCAGGCCCTG GAGGCCATTT ACGGCTCGGA CTTCCAGGAT
    CTGCGGCCGG ATGCGCGCGG ACGGCAACGT GAAATCCTCC ACGAGATTCA GAGAAGGAAA
    GAGGAGATCA AGGAAGAGAA GAAAAGGAAG GAAATGGCTA AGCAGGAACG TTTGGAAATC
    ACTAGCTTGA CAAACCAGGA CTATGCCTCT AAGAGAGACC CAGCAGGGCA CAGGGCAGCT
    GCCATCCTCC ATGGAGGCTC TCCTGACTTT GTAGGAAATG GTAAAGCCCG GACATACTCC
    TCAGGAAGGT CCAGGCGAGA ACGTCAGTAT TCTGTGTGTA GTGGTGAACC CTCTCCTGGC
    TCGTGTGACA TCCTCCATTT CTCTGTGGGT AGCCCTGATC AGCTCATGGT GCACAAAGGG
    AGATGTGTTG GCAGTGATGA GCAGCTGGGA AAGGTGGTAT ACAATGCTTT GGAAACAGCC
    ACGGGCAGCT TTGTCTTGCT GCATGAGTGG GTCCTTCAAT GGCAGAAGAT GGGCCCTTGC
    CTTACCAGTC AAGAGAAAGA GAAGATTGAC AAGTGCAAAA GGCAGATTCA AGGAGCAGAA
    ACAGAATTCA GCTCCCTAGT GAAACTGAGC CATCCAAATA TCGTCCGCTA CTTTGCGATG
    AACTCCAGAG AGGAGGAGGA CTCCATTGTG ATCGACATTC TGGCAGAGCA CGTCAGTGGC
    ATCTCTCTGG CTACACACCT GAGCCACTCG GGCCCAGTCC CCGCCCACCA GCTGCGCAAG
    TACACAGCCC AGCTCCTGGC AGGCCTGGAT TACCTACACA GCAACTCCGT GGTGCACAAG
    GTTCTGAGCG CGTCCAGTGT CTTGGTAGAT GCCGAGGGCA CTGTCAAGAT AACAGACTAC
    AGCATCTCTA AGCGTCTGGC AGACATTTGC AAGGAGGATG TATTTGAGCA AGCTCGAGTT
    CGTTTTAGTG ACAGTGCCCT GCCTTATAAA ACAGGAAAGA AAGGGGATGT GTGGCGGCTC
    GGCCTCCTGC TGTTGTCTCT CAGCCAAGGA CAGGAGTGTG GGGAGTATCC GGTGACCATC
    CCCAGTGACC TGCCAGCTGA CTTCCAAGAC TTCCTGAAGA AGTGTGTCTG CCTGGACGAC
    AAGGAAAGAT GGAGCCCTCA GCAGCTGCTG AAACACAGCT TTATAAACCC TCAACCAAAG
    CTGCCTTTGG TGGAGCAGAG TCCTGAAGAC TCTGGGGGAC AAGACTACAT TGAGACCGTC
    ATTCCCAGCA ACCAGCTGCC CAGCGCTGCG TTCTTCAGTG AGACACAGAA ACAGTTCTCC
    CGGTACTTCA TTGAGTTTGA AGAACTACAA CTCCTAGGGA AAGGAGCTTT TGGAGCTGTC
    ATCAAGGTGC AAAACAAGCT TGATGGCTGC TGCTATGCTG TGAAGCGCAT CCCTATCAAC
    CCTGCAAGCA GACACTTCCG CCGGATCAAG GGCGAGGTGA CACTGCTATC GCGCCTGCAC
    CATGAGAACA TTGTGCGCTA CTACAACGCC TGGATCGAGC GGCATGAGCG CCCAGCGGTG
    CCAGGGACAC CGCCCCCAGA CTGCACACCC CAGGCCCAGG ACAGCCCAGC CACCTGCGGG
    AAAACATCAG GCGACACTGA AGAACTGGGC AGCGTGGAGG CTGCAGCACC GCCACCCATC
    CTCAGCAGCT CGGTGGAGTG GAGCACATCT GCAGAGCGTT CTACCAGCAC CCGCTTCCCA
    GTCACGGGCC AGGATTCCAG CAGCGATGAG GAGGACGAGG ACGAGCGCGA TGGCGTCTTC
    TCCCAGTCCT TTCTACCTGC TTCAGATTCT GACAGTGACA TCATCTTTGA CAATGAAGAT
    GAAAACAGTA AAAGTCAAAA TCAGGATGAA GACTGCAATC AAAAGGACGG TAGCCATGAG
    ATTGAGCCTT CAGTGACAGC TGAGGCTGTG CACTACCTTT ATATCCAGAT GGAGTACTGC
    GAGAAGAGCA CGCTGCGGGA CACCATTGAC CAGGGGCTGT TCCGAGACAC CAGCCGGCTC
    TGGAGGCTTT TCCGAGAGAT TCTGGATGGA TTAGCTTATA TCCATGAGAA AGGAATGATT
    CATCGCGACT TGAAGCCTGT CAATATTTTT TTGGATTCTG ATGACCATGT GAAAATAGGT
    GACTTTGGCC TGGCAACAGA CCATCTGGCC TTCACTGCTG AAGGTAAACA GGACGACCAG
    GCAGGTGACG GAGTGATTAA GTCAGACCCT TCAGGCCATT TGACTGGCAT GGTTGGTACT
    GCTCTGTATG TAAGCCCTGA GGTCCAAGGA AGCACCAAGT CTGCATACAA CCAGAAAGTG
    GATCTCTTCA GCCTGGGAAT TATCTTCTTT GAGATGTCTT ATCACCCGAT GGTGACTGCC
    TCAGAAAGAA TTTTCGTTCT CAACCAACTT CGAGATCCCA CATCGCCCAA GTTTCCAGAC
    GACTTCGATG ATGGAGAACA TACAAAGCAG AAATCTGTCA TCTCCTGGCT GTTGAACCAT
    GATCCAGCTA AACGGCCCAC GGCCATGGAA CTGCTCAAGA GTGAGCTGCT CCCCCCGCCG
    CAGATGGAGG AGTCCGAGCT GCATGAAGTG CTGCACCACA CGCTGGCCAA CATAGATGGG
    AAGGCCTACC GCACCATGAT GAGTCAGATC TTCTGCCAGC ACATCTCCCC CGCTATAGAC
    TACACCTACG ACAGTGACAT CCTGAAGGGC AACTTCTTGA TTCGTACAGC CAAGATCCAG
    CAGCTTGTAT GTGAAACCAT CGTCCGCGTC TTTAAAAGGC ATGGCGCTGT CCAGCTGTGC
    ACCCCACTGC TGCTTCCCCG AAACAGGCAA ATATATGAGC ACAACGAAGC GGCTTTGTTC
    ATGGATCACA GCGGCATGCT GGTGATGCTT CCGTTCGACC TGCGGGTCCC TTTTGCAAGA
    TATGTGGCAA GAAATAACAT ACTGAATTTA AAACGATACT GCATAGAGCG GGTGTTCAGA
    CCTCGGAAGT TAGACCGATT TCATCCCAAA GAACTCCTGG AATGTGCATT TGATATCGTC
    ACTTCTACCA CCAACAGCTC TCTGCCCACT GCCGAAACCA TCTACACCAT CTATGAAATA
    ATCCAGGAGT TTCCTGCACT TCAGGAAAGG AATTACAGCA TTTACTTGAA CCATACGATG
    CTTCTGAAGG CAATACTCCT ACACTGTGGG ATCCCAGAGG ACAAGCTCAG CCAAGTCTAC
    GTCATTCTGT ATGATGCTGT GACAGAGAAG CTGACTAGGA GAGAAGTGGA AGCTAAATTC
    TGTAACCTGT CCTTGTCTTC TAATAGCCTG TGTAGACTCT ACAAGTTCAT TGAGCAGAAG
    GGGGATTTGC AAGACTTAAC GCCAACCATC AACTCACTAA TAAAACAGAA AACAGGCGTT
    GCTCAGTTGG TGAAGTATAG CTTAAAAGAC CTCGAGGACG TCGTCGGGCT GCTGAAGAAA
    CTTGGCGTGA AGTTACAGGT CTCCATCAAC CTGGGCTTGG TCTACAAGGT GCAGCAGCAC
    ACTGGCATCA TCTTCCAGTT CCTGGCATTC AGCAAACGCA GGCAAAGGGT TGTGCCTGAG
    ATCCTTGCCG CCGGTGGCAG ATACGACCTG CTGATTCCCA AGTTCAGAGG CCCACAGACT
    GTGGGGCCAG TCCCCACTGC TGTCGGTGTC AGCATAGCCA TAGACAAGAT ATTTGCTGCT
    GTCCTCAACA TGGAAGAGCC TGTTACAGTG AGCTCCTGTG ACCTCCTGGT TGTCAGTGTT
    GGCCAGATGT CCATGTCCAG GGCCATCAAC CTAACCCAGA AACTGTGGAC AGCGGGCATC
    ACTGCAGAAA TCATGTATGA CTGGTCCCAG TCCCAGGAAG AGTTACAAGA GTACTGCAGA
    CACCATGAAA TCACCTATGT GGCTCTGGTC TCCGATAAAG AAGGAAGCCA TGTCAAGGTC
    AAGTCCTTTG AGAAGGAGAG GCAAACAGAA AAGCGTGTAT TGGAATCGGA TCTTGTGGAT
    CACGTTATGC AGAAACTAAG AACCAAAGTT GGTGACGAAA GAAATTTCAG AGACGCTTCT
    GATAATCTTG CCGTACAAAC TCTGAAGGGG TCGTTTTCTA ATGCTTCAGG TTTGTTTGAA
    ATCCATGGAA CTACAGTAGT CCCCAACGTG ATTGTTCTAG CACCAGAAAA GCTGTCAGCC
    AGCACCAGGA GGCGACATGA GATTCAGGTG CAAACCCGAC TTCAGACTAC ACTTGCCAAC
    TTACATCAGA AAAGCAGTGA AATTGAAATT TTGGCTGTGG ACCTACCCAA GGAAACAATC
    TTACAGTTTC TATCATTAGA GTGGGATGCT GATGAACAGG CATTTAACAC AACTGTGAAG
    CAGCTGCTGT CACGCCTGCC AAAGCAAAGA TACCTCAAAC TCGTCTGCGA TGAAATTTAT
    AACATCAAAG TTGAAAAGAA GGTGTCAGTG CTGTTCCTGT ACAGCTACAG AGATGACTAC
    TACAGAATCC TATTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu80270
    Clone ID Related Accession (Same CDS sequence) XM_017318909.1 , XM_017318909.2
    Accession Version XM_017318909.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4866bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product eIF-2-alpha kinase GCN2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCTGGGG GCCGCGGGGC CTCTGGACGC GGCCGGGCTG AGCCACAGGA GAGCTATTCG 
    CAGCGACAGG ACCACGAGCT GCAGGCCCTG GAGGCCATTT ACGGCTCGGA CTTCCAGGAT
    CTGCGGCCGG ATGCGCGCGG ACGGGTGAGA GAGCCTCCTG AAATCAACTT AGTTCTTTAC
    CCTCAGGGCC TAGCTGGTGA AGAAGTATAC GTGCAAGTGG AACTGAGGGT TAAATGCCCA
    CCTACATACC CAGATGTAGT TCCCGAAATA GACTTAAAAA ATGCTAAAGG CCTGTCGAAT
    GAAAGTGTTA ATTTGTTAAA ATCACACCTG GAAGAACTGG CCAAAAAGCA GTGTGGAGAG
    GTGATGATAT TTGAACTGGC ACACCATGTG CAGTCGTTTC TCAGCGAGCA TAACAAGCCC
    CCACCAAAGT CTTTCCATGA AGAAATGCTG GAAAGGCAGG CTCAGGAGAA GCAGCAGAGG
    TTGCTGGAGG CCAGGCGGAA GGAGGAGCAG GAGGAACGTT TGGAAATCAC TAGCTTGACA
    AACCAGGACT ATGCCTCTAA GAGAGACCCA GCAGGGCACA GGGCAGCTGC CATCCTCCAT
    GGAGGCTCTC CTGACTTTGT AGGAAATGGT AAAGCCCGGA CATACTCCTC AGGAAGGTCC
    AGGCGAGAAC GTCAGTATTC TGTGTGTAGT GGTGAACCCT CTCCTGGCTC GTGTGACATC
    CTCCATTTCT CTGTGGGTAG CCCTGATCAG CTCATGGTGC ACAAAGGGAG ATGTGTTGGC
    AGTGATGAGC AGCTGGGAAA GGTGGTATAC AATGCTTTGG AAACAGCCAC GGGCAGCTTT
    GTCTTGCTGC ATGAGTGGGT CCTTCAATGG CAGAAGATGG GCCCTTGCCT TACCAGTCAA
    GAGAAAGAGA AGATTGACAA GTGCAAAAGG CAGATTCAAG GAGCAGAAAC AGAATTCAGC
    TCCCTAGTGA AACTGAGCCA TCCAAATATC GTCCGCTACT TTGCGATGAA CTCCAGAGAG
    GAGGAGGACT CCATTGTGAT CGACATTCTG GCAGAGCACG TCAGTGGCAT CTCTCTGGCT
    ACACACCTGA GCCACTCGGG CCCAGTCCCC GCCCACCAGC TGCGCAAGTA CACAGCCCAG
    CTCCTGGCAG GCCTGGATTA CCTACACAGC AACTCCGTGG TGCACAAGGT TCTGAGCGCG
    TCCAGTGTCT TGGTAGATGC CGAGGGCACT GTCAAGATAA CAGACTACAG CATCTCTAAG
    CGTCTGGCAG ACATTTGCAA GGAGGATGTA TTTGAGCAAG CTCGAGTTCG TTTTAGTGAC
    AGTGCCCTGC CTTATAAAAC AGGAAAGAAA GGGGATGTGT GGCGGCTCGG CCTCCTGCTG
    TTGTCTCTCA GCCAAGGACA GGAGTGTGGG GAGTATCCGG TGACCATCCC CAGTGACCTG
    CCAGCTGACT TCCAAGACTT CCTGAAGAAG TGTGTCTGCC TGGACGACAA GGAAAGATGG
    AGCCCTCAGC AGCTGCTGAA ACACAGCTTT ATAAACCCTC AACCAAAGCT GCCTTTGGTG
    GAGCAGAGTC CTGAAGACTC TGGGGGACAA GACTACATTG AGACCGTCAT TCCCAGCAAC
    CAGCTGCCCA GCGCTGCGTT CTTCAGTGAG ACACAGAAAC AGTTCTCCCG GTACTTCATT
    GAGTTTGAAG AACTACAACT CCTAGGGAAA GGAGCTTTTG GAGCTGTCAT CAAGGTGCAA
    AACAAGCTTG ATGGCTGCTG CTATGCTGTG AAGCGCATCC CTATCAACCC TGCAAGCAGA
    CACTTCCGCC GGATCAAGGG CGAGGTGACA CTGCTATCGC GCCTGCACCA TGAGAACATT
    GTGCGCTACT ACAACGCCTG GATCGAGCGG CATGAGCGCC CAGCGGTGCC AGGGACACCG
    CCCCCAGACT GCACACCCCA GGCCCAGGAC AGCCCAGCCA CCTGCGGGAA AACATCAGGC
    GACACTGAAG AACTGGGCAG CGTGGAGGCT GCAGCACCGC CACCCATCCT CAGCAGCTCG
    GTGGAGTGGA GCACATCTGC AGAGCGTTCT ACCAGCACCC GCTTCCCAGT CACGGGCCAG
    GATTCCAGCA GCGATGAGGA GGACGAGGAC GAGCGCGATG GCGTCTTCTC CCAGTCCTTT
    CTACCTGCTT CAGATTCTGA CAGTGACATC ATCTTTGACA ATGAAGATGA AAACAGTAAA
    AGTCAAAATC AGGATGAAGA CTGCAATCAA AAGGACGGTA GCCATGAGAT TGAGCCTTCA
    GTGACAGCTG AGGCTGTGCA CTACCTTTAT ATCCAGATGG AGTACTGCGA GAAGAGCACG
    CTGCGGGACA CCATTGACCA GGGGCTGTTC CGAGACACCA GCCGGCTCTG GAGGCTTTTC
    CGAGAGATTC TGGATGGATT AGCTTATATC CATGAGAAAG GAATGATTCA TCGCGACTTG
    AAGCCTGTCA ATATTTTTTT GGATTCTGAT GACCATGTGA AAATAGGTGA CTTTGGCCTG
    GCAACAGACC ATCTGGCCTT CACTGCTGAA GGTAAACAGG ACGACCAGGC AGGTGACGGA
    GTGATTAAGT CAGACCCTTC AGGCCATTTG ACTGGCATGG TTGGTACTGC TCTGTATGTA
    AGCCCTGAGG TCCAAGGAAG CACCAAGTCT GCATACAACC AGAAAGTGGA TCTCTTCAGC
    CTGGGAATTA TCTTCTTTGA GATGTCTTAT CACCCGATGG TGACTGCCTC AGAAAGAATT
    TTCGTTCTCA ACCAACTTCG AGATCCCACA TCGCCCAAGT TTCCAGACGA CTTCGATGAT
    GGAGAACATA CAAAGCAGAA ATCTGTCATC TCCTGGCTGT TGAACCATGA TCCAGCTAAA
    CGGCCCACGG CCATGGAACT GCTCAAGAGT GAGCTGCTCC CCCCGCCGCA GATGGAGGAG
    TCCGAGCTGC ATGAAGTGCT GCACCACACG CTGGCCAACA TAGATGGGAA GGCCTACCGC
    ACCATGATGA GTCAGATCTT CTGCCAGCAC ATCTCCCCCG CTATAGACTA CACCTACGAC
    AGTGACATCC TGAAGGGCAA CTTCTTGATT CGTACAGCCA AGATCCAGCA GCTTGTATGT
    GAAACCATCG TCCGCGTCTT TAAAAGGCAT GGCGCTGTCC AGCTGTGCAC CCCACTGCTG
    CTTCCCCGAA ACAGGCAAAT ATATGAGCAC AACGAAGCGG CTTTGTTCAT GGATCACAGC
    GGCATGCTGG TGATGCTTCC GTTCGACCTG CGGGTCCCTT TTGCAAGATA TGTGGCAAGA
    AATAACATAC TGAATTTAAA ACGATACTGC ATAGAGCGGG TGTTCAGACC TCGGAAGTTA
    GACCGATTTC ATCCCAAAGA ACTCCTGGAA TGTGCATTTG ATATCGTCAC TTCTACCACC
    AACAGCTCTC TGCCCACTGC CGAAACCATC TACACCATCT ATGAAATAAT CCAGGAGTTT
    CCTGCACTTC AGGAAAGGAA TTACAGCATT TACTTGAACC ATACGATGCT TCTGAAGGCA
    ATACTCCTAC ACTGTGGGAT CCCAGAGGAC AAGCTCAGCC AAGTCTACGT CATTCTGTAT
    GATGCTGTGA CAGAGAAGCT GACTAGGAGA GAAGTGGAAG CTAAATTCTG TAACCTGTCC
    TTGTCTTCTA ATAGCCTGTG TAGACTCTAC AAGTTCATTG AGCAGAAGGG GGATTTGCAA
    GACTTAACGC CAACCATCAA CTCACTAATA AAACAGAAAA CAGGCGTTGC TCAGTTGGTG
    AAGTATAGCT TAAAAGACCT CGAGGACGTC GTCGGGCTGC TGAAGAAACT TGGCGTGAAG
    TTACAGGTCT CCATCAACCT GGGCTTGGTC TACAAGGTGC AGCAGCACAC TGGCATCATC
    TTCCAGTTCC TGGCATTCAG CAAACGCAGG CAAAGGGTTG TGCCTGAGAT CCTTGCCGCC
    GGTGGCAGAT ACGACCTGCT GATTCCCAAG TTCAGAGGCC CACAGACTGT GGGGCCAGTC
    CCCACTGCTG TCGGTGTCAG CATAGCCATA GACAAGATAT TTGCTGCTGT CCTCAACATG
    GAAGAGCCTG TTACAGTGAG CTCCTGTGAC CTCCTGGTTG TCAGTGTTGG CCAGATGTCC
    ATGTCCAGGG CCATCAACCT AACCCAGAAA CTGTGGACAG CGGGCATCAC TGCAGAAATC
    ATGTATGACT GGTCCCAGTC CCAGGAAGAG TTACAAGAGT ACTGCAGACA CCATGAAATC
    ACCTATGTGG CTCTGGTCTC CGATAAAGAA GGAAGCCATG TCAAGGTCAA GTCCTTTGAG
    AAGGAGAGGC AAACAGAAAA GCGTGTATTG GAATCGGATC TTGTGGATCA CGTTATGCAG
    AAACTAAGAA CCAAAGTTGG TGACGAAAGA AATTTCAGAG ACGCTTCTGA TAATCTTGCC
    GTACAAACTC TGAAGGGGTC GTTTTCTAAT GCTTCAGGTT TGTTTGAAAT CCATGGAACT
    ACAGTAGTCC CCAACGTGAT TGTTCTAGCA CCAGAAAAGC TGTCAGCCAG CACCAGGAGG
    CGACATGAGA TTCAGGTGCA AACCCGACTT CAGACTACAC TTGCCAACTT ACATCAGAAA
    AGCAGTGAAA TTGAAATTTT GGCTGTGGAC CTACCCAAGG AAACAATCTT ACAGTTTCTA
    TCATTAGAGT GGGATGCTGA TGAACAGGCA TTTAACACAA CTGTGAAGCA GCTGCTGTCA
    CGCCTGCCAA AGCAAAGATA CCTCAAACTC GTCTGCGATG AAATTTATAA CATCAAAGTT
    GAAAAGAAGG TGTCAGTGCT GTTCCTGTAC AGCTACAGAG ATGACTACTA CAGAATCCTA
    TTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017174398.1
    CDS129..4994
    Translation

    Target ORF information:

    RefSeq Version XM_017318909.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017318909.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCTGGGG GCCGCGGGGC CTCTGGACGC GGCCGGGCTG AGCCACAGGA GAGCTATTCG 
    CAGCGACAGG ACCACGAGCT GCAGGCCCTG GAGGCCATTT ACGGCTCGGA CTTCCAGGAT
    CTGCGGCCGG ATGCGCGCGG ACGGGTGAGA GAGCCTCCTG AAATCAACTT AGTTCTTTAC
    CCTCAGGGCC TAGCTGGTGA AGAAGTATAC GTGCAAGTGG AACTGAGGGT TAAATGCCCA
    CCTACATACC CAGATGTAGT TCCCGAAATA GACTTAAAAA ATGCTAAAGG CCTGTCGAAT
    GAAAGTGTTA ATTTGTTAAA ATCACACCTG GAAGAACTGG CCAAAAAGCA GTGTGGAGAG
    GTGATGATAT TTGAACTGGC ACACCATGTG CAGTCGTTTC TCAGCGAGCA TAACAAGCCC
    CCACCAAAGT CTTTCCATGA AGAAATGCTG GAAAGGCAGG CTCAGGAGAA GCAGCAGAGG
    TTGCTGGAGG CCAGGCGGAA GGAGGAGCAG GAGGAACGTT TGGAAATCAC TAGCTTGACA
    AACCAGGACT ATGCCTCTAA GAGAGACCCA GCAGGGCACA GGGCAGCTGC CATCCTCCAT
    GGAGGCTCTC CTGACTTTGT AGGAAATGGT AAAGCCCGGA CATACTCCTC AGGAAGGTCC
    AGGCGAGAAC GTCAGTATTC TGTGTGTAGT GGTGAACCCT CTCCTGGCTC GTGTGACATC
    CTCCATTTCT CTGTGGGTAG CCCTGATCAG CTCATGGTGC ACAAAGGGAG ATGTGTTGGC
    AGTGATGAGC AGCTGGGAAA GGTGGTATAC AATGCTTTGG AAACAGCCAC GGGCAGCTTT
    GTCTTGCTGC ATGAGTGGGT CCTTCAATGG CAGAAGATGG GCCCTTGCCT TACCAGTCAA
    GAGAAAGAGA AGATTGACAA GTGCAAAAGG CAGATTCAAG GAGCAGAAAC AGAATTCAGC
    TCCCTAGTGA AACTGAGCCA TCCAAATATC GTCCGCTACT TTGCGATGAA CTCCAGAGAG
    GAGGAGGACT CCATTGTGAT CGACATTCTG GCAGAGCACG TCAGTGGCAT CTCTCTGGCT
    ACACACCTGA GCCACTCGGG CCCAGTCCCC GCCCACCAGC TGCGCAAGTA CACAGCCCAG
    CTCCTGGCAG GCCTGGATTA CCTACACAGC AACTCCGTGG TGCACAAGGT TCTGAGCGCG
    TCCAGTGTCT TGGTAGATGC CGAGGGCACT GTCAAGATAA CAGACTACAG CATCTCTAAG
    CGTCTGGCAG ACATTTGCAA GGAGGATGTA TTTGAGCAAG CTCGAGTTCG TTTTAGTGAC
    AGTGCCCTGC CTTATAAAAC AGGAAAGAAA GGGGATGTGT GGCGGCTCGG CCTCCTGCTG
    TTGTCTCTCA GCCAAGGACA GGAGTGTGGG GAGTATCCGG TGACCATCCC CAGTGACCTG
    CCAGCTGACT TCCAAGACTT CCTGAAGAAG TGTGTCTGCC TGGACGACAA GGAAAGATGG
    AGCCCTCAGC AGCTGCTGAA ACACAGCTTT ATAAACCCTC AACCAAAGCT GCCTTTGGTG
    GAGCAGAGTC CTGAAGACTC TGGGGGACAA GACTACATTG AGACCGTCAT TCCCAGCAAC
    CAGCTGCCCA GCGCTGCGTT CTTCAGTGAG ACACAGAAAC AGTTCTCCCG GTACTTCATT
    GAGTTTGAAG AACTACAACT CCTAGGGAAA GGAGCTTTTG GAGCTGTCAT CAAGGTGCAA
    AACAAGCTTG ATGGCTGCTG CTATGCTGTG AAGCGCATCC CTATCAACCC TGCAAGCAGA
    CACTTCCGCC GGATCAAGGG CGAGGTGACA CTGCTATCGC GCCTGCACCA TGAGAACATT
    GTGCGCTACT ACAACGCCTG GATCGAGCGG CATGAGCGCC CAGCGGTGCC AGGGACACCG
    CCCCCAGACT GCACACCCCA GGCCCAGGAC AGCCCAGCCA CCTGCGGGAA AACATCAGGC
    GACACTGAAG AACTGGGCAG CGTGGAGGCT GCAGCACCGC CACCCATCCT CAGCAGCTCG
    GTGGAGTGGA GCACATCTGC AGAGCGTTCT ACCAGCACCC GCTTCCCAGT CACGGGCCAG
    GATTCCAGCA GCGATGAGGA GGACGAGGAC GAGCGCGATG GCGTCTTCTC CCAGTCCTTT
    CTACCTGCTT CAGATTCTGA CAGTGACATC ATCTTTGACA ATGAAGATGA AAACAGTAAA
    AGTCAAAATC AGGATGAAGA CTGCAATCAA AAGGACGGTA GCCATGAGAT TGAGCCTTCA
    GTGACAGCTG AGGCTGTGCA CTACCTTTAT ATCCAGATGG AGTACTGCGA GAAGAGCACG
    CTGCGGGACA CCATTGACCA GGGGCTGTTC CGAGACACCA GCCGGCTCTG GAGGCTTTTC
    CGAGAGATTC TGGATGGATT AGCTTATATC CATGAGAAAG GAATGATTCA TCGCGACTTG
    AAGCCTGTCA ATATTTTTTT GGATTCTGAT GACCATGTGA AAATAGGTGA CTTTGGCCTG
    GCAACAGACC ATCTGGCCTT CACTGCTGAA GGTAAACAGG ACGACCAGGC AGGTGACGGA
    GTGATTAAGT CAGACCCTTC AGGCCATTTG ACTGGCATGG TTGGTACTGC TCTGTATGTA
    AGCCCTGAGG TCCAAGGAAG CACCAAGTCT GCATACAACC AGAAAGTGGA TCTCTTCAGC
    CTGGGAATTA TCTTCTTTGA GATGTCTTAT CACCCGATGG TGACTGCCTC AGAAAGAATT
    TTCGTTCTCA ACCAACTTCG AGATCCCACA TCGCCCAAGT TTCCAGACGA CTTCGATGAT
    GGAGAACATA CAAAGCAGAA ATCTGTCATC TCCTGGCTGT TGAACCATGA TCCAGCTAAA
    CGGCCCACGG CCATGGAACT GCTCAAGAGT GAGCTGCTCC CCCCGCCGCA GATGGAGGAG
    TCCGAGCTGC ATGAAGTGCT GCACCACACG CTGGCCAACA TAGATGGGAA GGCCTACCGC
    ACCATGATGA GTCAGATCTT CTGCCAGCAC ATCTCCCCCG CTATAGACTA CACCTACGAC
    AGTGACATCC TGAAGGGCAA CTTCTTGATT CGTACAGCCA AGATCCAGCA GCTTGTATGT
    GAAACCATCG TCCGCGTCTT TAAAAGGCAT GGCGCTGTCC AGCTGTGCAC CCCACTGCTG
    CTTCCCCGAA ACAGGCAAAT ATATGAGCAC AACGAAGCGG CTTTGTTCAT GGATCACAGC
    GGCATGCTGG TGATGCTTCC GTTCGACCTG CGGGTCCCTT TTGCAAGATA TGTGGCAAGA
    AATAACATAC TGAATTTAAA ACGATACTGC ATAGAGCGGG TGTTCAGACC TCGGAAGTTA
    GACCGATTTC ATCCCAAAGA ACTCCTGGAA TGTGCATTTG ATATCGTCAC TTCTACCACC
    AACAGCTCTC TGCCCACTGC CGAAACCATC TACACCATCT ATGAAATAAT CCAGGAGTTT
    CCTGCACTTC AGGAAAGGAA TTACAGCATT TACTTGAACC ATACGATGCT TCTGAAGGCA
    ATACTCCTAC ACTGTGGGAT CCCAGAGGAC AAGCTCAGCC AAGTCTACGT CATTCTGTAT
    GATGCTGTGA CAGAGAAGCT GACTAGGAGA GAAGTGGAAG CTAAATTCTG TAACCTGTCC
    TTGTCTTCTA ATAGCCTGTG TAGACTCTAC AAGTTCATTG AGCAGAAGGG GGATTTGCAA
    GACTTAACGC CAACCATCAA CTCACTAATA AAACAGAAAA CAGGCGTTGC TCAGTTGGTG
    AAGTATAGCT TAAAAGACCT CGAGGACGTC GTCGGGCTGC TGAAGAAACT TGGCGTGAAG
    TTACAGGTCT CCATCAACCT GGGCTTGGTC TACAAGGTGC AGCAGCACAC TGGCATCATC
    TTCCAGTTCC TGGCATTCAG CAAACGCAGG CAAAGGGTTG TGCCTGAGAT CCTTGCCGCC
    GGTGGCAGAT ACGACCTGCT GATTCCCAAG TTCAGAGGCC CACAGACTGT GGGGCCAGTC
    CCCACTGCTG TCGGTGTCAG CATAGCCATA GACAAGATAT TTGCTGCTGT CCTCAACATG
    GAAGAGCCTG TTACAGTGAG CTCCTGTGAC CTCCTGGTTG TCAGTGTTGG CCAGATGTCC
    ATGTCCAGGG CCATCAACCT AACCCAGAAA CTGTGGACAG CGGGCATCAC TGCAGAAATC
    ATGTATGACT GGTCCCAGTC CCAGGAAGAG TTACAAGAGT ACTGCAGACA CCATGAAATC
    ACCTATGTGG CTCTGGTCTC CGATAAAGAA GGAAGCCATG TCAAGGTCAA GTCCTTTGAG
    AAGGAGAGGC AAACAGAAAA GCGTGTATTG GAATCGGATC TTGTGGATCA CGTTATGCAG
    AAACTAAGAA CCAAAGTTGG TGACGAAAGA AATTTCAGAG ACGCTTCTGA TAATCTTGCC
    GTACAAACTC TGAAGGGGTC GTTTTCTAAT GCTTCAGGTT TGTTTGAAAT CCATGGAACT
    ACAGTAGTCC CCAACGTGAT TGTTCTAGCA CCAGAAAAGC TGTCAGCCAG CACCAGGAGG
    CGACATGAGA TTCAGGTGCA AACCCGACTT CAGACTACAC TTGCCAACTT ACATCAGAAA
    AGCAGTGAAA TTGAAATTTT GGCTGTGGAC CTACCCAAGG AAACAATCTT ACAGTTTCTA
    TCATTAGAGT GGGATGCTGA TGAACAGGCA TTTAACACAA CTGTGAAGCA GCTGCTGTCA
    CGCCTGCCAA AGCAAAGATA CCTCAAACTC GTCTGCGATG AAATTTATAA CATCAAAGTT
    GAAAAGAAGG TGTCAGTGCT GTTCCTGTAC AGCTACAGAG ATGACTACTA CAGAATCCTA
    TTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu80270
    Clone ID Related Accession (Same CDS sequence) XM_017318909.1 , XM_017318909.2
    Accession Version XM_017318909.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4866bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product eIF-2-alpha kinase GCN2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017318909.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCTGGGG GCCGCGGGGC CTCTGGACGC GGCCGGGCTG AGCCACAGGA GAGCTATTCG 
    CAGCGACAGG ACCACGAGCT GCAGGCCCTG GAGGCCATTT ACGGCTCGGA CTTCCAGGAT
    CTGCGGCCGG ATGCGCGCGG ACGGGTGAGA GAGCCTCCTG AAATCAACTT AGTTCTTTAC
    CCTCAGGGCC TAGCTGGTGA AGAAGTATAC GTGCAAGTGG AACTGAGGGT TAAATGCCCA
    CCTACATACC CAGATGTAGT TCCCGAAATA GACTTAAAAA ATGCTAAAGG CCTGTCGAAT
    GAAAGTGTTA ATTTGTTAAA ATCACACCTG GAAGAACTGG CCAAAAAGCA GTGTGGAGAG
    GTGATGATAT TTGAACTGGC ACACCATGTG CAGTCGTTTC TCAGCGAGCA TAACAAGCCC
    CCACCAAAGT CTTTCCATGA AGAAATGCTG GAAAGGCAGG CTCAGGAGAA GCAGCAGAGG
    TTGCTGGAGG CCAGGCGGAA GGAGGAGCAG GAGGAACGTT TGGAAATCAC TAGCTTGACA
    AACCAGGACT ATGCCTCTAA GAGAGACCCA GCAGGGCACA GGGCAGCTGC CATCCTCCAT
    GGAGGCTCTC CTGACTTTGT AGGAAATGGT AAAGCCCGGA CATACTCCTC AGGAAGGTCC
    AGGCGAGAAC GTCAGTATTC TGTGTGTAGT GGTGAACCCT CTCCTGGCTC GTGTGACATC
    CTCCATTTCT CTGTGGGTAG CCCTGATCAG CTCATGGTGC ACAAAGGGAG ATGTGTTGGC
    AGTGATGAGC AGCTGGGAAA GGTGGTATAC AATGCTTTGG AAACAGCCAC GGGCAGCTTT
    GTCTTGCTGC ATGAGTGGGT CCTTCAATGG CAGAAGATGG GCCCTTGCCT TACCAGTCAA
    GAGAAAGAGA AGATTGACAA GTGCAAAAGG CAGATTCAAG GAGCAGAAAC AGAATTCAGC
    TCCCTAGTGA AACTGAGCCA TCCAAATATC GTCCGCTACT TTGCGATGAA CTCCAGAGAG
    GAGGAGGACT CCATTGTGAT CGACATTCTG GCAGAGCACG TCAGTGGCAT CTCTCTGGCT
    ACACACCTGA GCCACTCGGG CCCAGTCCCC GCCCACCAGC TGCGCAAGTA CACAGCCCAG
    CTCCTGGCAG GCCTGGATTA CCTACACAGC AACTCCGTGG TGCACAAGGT TCTGAGCGCG
    TCCAGTGTCT TGGTAGATGC CGAGGGCACT GTCAAGATAA CAGACTACAG CATCTCTAAG
    CGTCTGGCAG ACATTTGCAA GGAGGATGTA TTTGAGCAAG CTCGAGTTCG TTTTAGTGAC
    AGTGCCCTGC CTTATAAAAC AGGAAAGAAA GGGGATGTGT GGCGGCTCGG CCTCCTGCTG
    TTGTCTCTCA GCCAAGGACA GGAGTGTGGG GAGTATCCGG TGACCATCCC CAGTGACCTG
    CCAGCTGACT TCCAAGACTT CCTGAAGAAG TGTGTCTGCC TGGACGACAA GGAAAGATGG
    AGCCCTCAGC AGCTGCTGAA ACACAGCTTT ATAAACCCTC AACCAAAGCT GCCTTTGGTG
    GAGCAGAGTC CTGAAGACTC TGGGGGACAA GACTACATTG AGACCGTCAT TCCCAGCAAC
    CAGCTGCCCA GCGCTGCGTT CTTCAGTGAG ACACAGAAAC AGTTCTCCCG GTACTTCATT
    GAGTTTGAAG AACTACAACT CCTAGGGAAA GGAGCTTTTG GAGCTGTCAT CAAGGTGCAA
    AACAAGCTTG ATGGCTGCTG CTATGCTGTG AAGCGCATCC CTATCAACCC TGCAAGCAGA
    CACTTCCGCC GGATCAAGGG CGAGGTGACA CTGCTATCGC GCCTGCACCA TGAGAACATT
    GTGCGCTACT ACAACGCCTG GATCGAGCGG CATGAGCGCC CAGCGGTGCC AGGGACACCG
    CCCCCAGACT GCACACCCCA GGCCCAGGAC AGCCCAGCCA CCTGCGGGAA AACATCAGGC
    GACACTGAAG AACTGGGCAG CGTGGAGGCT GCAGCACCGC CACCCATCCT CAGCAGCTCG
    GTGGAGTGGA GCACATCTGC AGAGCGTTCT ACCAGCACCC GCTTCCCAGT CACGGGCCAG
    GATTCCAGCA GCGATGAGGA GGACGAGGAC GAGCGCGATG GCGTCTTCTC CCAGTCCTTT
    CTACCTGCTT CAGATTCTGA CAGTGACATC ATCTTTGACA ATGAAGATGA AAACAGTAAA
    AGTCAAAATC AGGATGAAGA CTGCAATCAA AAGGACGGTA GCCATGAGAT TGAGCCTTCA
    GTGACAGCTG AGGCTGTGCA CTACCTTTAT ATCCAGATGG AGTACTGCGA GAAGAGCACG
    CTGCGGGACA CCATTGACCA GGGGCTGTTC CGAGACACCA GCCGGCTCTG GAGGCTTTTC
    CGAGAGATTC TGGATGGATT AGCTTATATC CATGAGAAAG GAATGATTCA TCGCGACTTG
    AAGCCTGTCA ATATTTTTTT GGATTCTGAT GACCATGTGA AAATAGGTGA CTTTGGCCTG
    GCAACAGACC ATCTGGCCTT CACTGCTGAA GGTAAACAGG ACGACCAGGC AGGTGACGGA
    GTGATTAAGT CAGACCCTTC AGGCCATTTG ACTGGCATGG TTGGTACTGC TCTGTATGTA
    AGCCCTGAGG TCCAAGGAAG CACCAAGTCT GCATACAACC AGAAAGTGGA TCTCTTCAGC
    CTGGGAATTA TCTTCTTTGA GATGTCTTAT CACCCGATGG TGACTGCCTC AGAAAGAATT
    TTCGTTCTCA ACCAACTTCG AGATCCCACA TCGCCCAAGT TTCCAGACGA CTTCGATGAT
    GGAGAACATA CAAAGCAGAA ATCTGTCATC TCCTGGCTGT TGAACCATGA TCCAGCTAAA
    CGGCCCACGG CCATGGAACT GCTCAAGAGT GAGCTGCTCC CCCCGCCGCA GATGGAGGAG
    TCCGAGCTGC ATGAAGTGCT GCACCACACG CTGGCCAACA TAGATGGGAA GGCCTACCGC
    ACCATGATGA GTCAGATCTT CTGCCAGCAC ATCTCCCCCG CTATAGACTA CACCTACGAC
    AGTGACATCC TGAAGGGCAA CTTCTTGATT CGTACAGCCA AGATCCAGCA GCTTGTATGT
    GAAACCATCG TCCGCGTCTT TAAAAGGCAT GGCGCTGTCC AGCTGTGCAC CCCACTGCTG
    CTTCCCCGAA ACAGGCAAAT ATATGAGCAC AACGAAGCGG CTTTGTTCAT GGATCACAGC
    GGCATGCTGG TGATGCTTCC GTTCGACCTG CGGGTCCCTT TTGCAAGATA TGTGGCAAGA
    AATAACATAC TGAATTTAAA ACGATACTGC ATAGAGCGGG TGTTCAGACC TCGGAAGTTA
    GACCGATTTC ATCCCAAAGA ACTCCTGGAA TGTGCATTTG ATATCGTCAC TTCTACCACC
    AACAGCTCTC TGCCCACTGC CGAAACCATC TACACCATCT ATGAAATAAT CCAGGAGTTT
    CCTGCACTTC AGGAAAGGAA TTACAGCATT TACTTGAACC ATACGATGCT TCTGAAGGCA
    ATACTCCTAC ACTGTGGGAT CCCAGAGGAC AAGCTCAGCC AAGTCTACGT CATTCTGTAT
    GATGCTGTGA CAGAGAAGCT GACTAGGAGA GAAGTGGAAG CTAAATTCTG TAACCTGTCC
    TTGTCTTCTA ATAGCCTGTG TAGACTCTAC AAGTTCATTG AGCAGAAGGG GGATTTGCAA
    GACTTAACGC CAACCATCAA CTCACTAATA AAACAGAAAA CAGGCGTTGC TCAGTTGGTG
    AAGTATAGCT TAAAAGACCT CGAGGACGTC GTCGGGCTGC TGAAGAAACT TGGCGTGAAG
    TTACAGGTCT CCATCAACCT GGGCTTGGTC TACAAGGTGC AGCAGCACAC TGGCATCATC
    TTCCAGTTCC TGGCATTCAG CAAACGCAGG CAAAGGGTTG TGCCTGAGAT CCTTGCCGCC
    GGTGGCAGAT ACGACCTGCT GATTCCCAAG TTCAGAGGCC CACAGACTGT GGGGCCAGTC
    CCCACTGCTG TCGGTGTCAG CATAGCCATA GACAAGATAT TTGCTGCTGT CCTCAACATG
    GAAGAGCCTG TTACAGTGAG CTCCTGTGAC CTCCTGGTTG TCAGTGTTGG CCAGATGTCC
    ATGTCCAGGG CCATCAACCT AACCCAGAAA CTGTGGACAG CGGGCATCAC TGCAGAAATC
    ATGTATGACT GGTCCCAGTC CCAGGAAGAG TTACAAGAGT ACTGCAGACA CCATGAAATC
    ACCTATGTGG CTCTGGTCTC CGATAAAGAA GGAAGCCATG TCAAGGTCAA GTCCTTTGAG
    AAGGAGAGGC AAACAGAAAA GCGTGTATTG GAATCGGATC TTGTGGATCA CGTTATGCAG
    AAACTAAGAA CCAAAGTTGG TGACGAAAGA AATTTCAGAG ACGCTTCTGA TAATCTTGCC
    GTACAAACTC TGAAGGGGTC GTTTTCTAAT GCTTCAGGTT TGTTTGAAAT CCATGGAACT
    ACAGTAGTCC CCAACGTGAT TGTTCTAGCA CCAGAAAAGC TGTCAGCCAG CACCAGGAGG
    CGACATGAGA TTCAGGTGCA AACCCGACTT CAGACTACAC TTGCCAACTT ACATCAGAAA
    AGCAGTGAAA TTGAAATTTT GGCTGTGGAC CTACCCAAGG AAACAATCTT ACAGTTTCTA
    TCATTAGAGT GGGATGCTGA TGAACAGGCA TTTAACACAA CTGTGAAGCA GCTGCTGTCA
    CGCCTGCCAA AGCAAAGATA CCTCAAACTC GTCTGCGATG AAATTTATAA CATCAAAGTT
    GAAAAGAAGG TGTCAGTGCT GTTCCTGTAC AGCTACAGAG ATGACTACTA CAGAATCCTA
    TTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017174398.1
    CDS127..4992
    Translation

    Target ORF information:

    RefSeq Version XM_017318909.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017318909.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCTGGGG GCCGCGGGGC CTCTGGACGC GGCCGGGCTG AGCCACAGGA GAGCTATTCG 
    CAGCGACAGG ACCACGAGCT GCAGGCCCTG GAGGCCATTT ACGGCTCGGA CTTCCAGGAT
    CTGCGGCCGG ATGCGCGCGG ACGGGTGAGA GAGCCTCCTG AAATCAACTT AGTTCTTTAC
    CCTCAGGGCC TAGCTGGTGA AGAAGTATAC GTGCAAGTGG AACTGAGGGT TAAATGCCCA
    CCTACATACC CAGATGTAGT TCCCGAAATA GACTTAAAAA ATGCTAAAGG CCTGTCGAAT
    GAAAGTGTTA ATTTGTTAAA ATCACACCTG GAAGAACTGG CCAAAAAGCA GTGTGGAGAG
    GTGATGATAT TTGAACTGGC ACACCATGTG CAGTCGTTTC TCAGCGAGCA TAACAAGCCC
    CCACCAAAGT CTTTCCATGA AGAAATGCTG GAAAGGCAGG CTCAGGAGAA GCAGCAGAGG
    TTGCTGGAGG CCAGGCGGAA GGAGGAGCAG GAGGAACGTT TGGAAATCAC TAGCTTGACA
    AACCAGGACT ATGCCTCTAA GAGAGACCCA GCAGGGCACA GGGCAGCTGC CATCCTCCAT
    GGAGGCTCTC CTGACTTTGT AGGAAATGGT AAAGCCCGGA CATACTCCTC AGGAAGGTCC
    AGGCGAGAAC GTCAGTATTC TGTGTGTAGT GGTGAACCCT CTCCTGGCTC GTGTGACATC
    CTCCATTTCT CTGTGGGTAG CCCTGATCAG CTCATGGTGC ACAAAGGGAG ATGTGTTGGC
    AGTGATGAGC AGCTGGGAAA GGTGGTATAC AATGCTTTGG AAACAGCCAC GGGCAGCTTT
    GTCTTGCTGC ATGAGTGGGT CCTTCAATGG CAGAAGATGG GCCCTTGCCT TACCAGTCAA
    GAGAAAGAGA AGATTGACAA GTGCAAAAGG CAGATTCAAG GAGCAGAAAC AGAATTCAGC
    TCCCTAGTGA AACTGAGCCA TCCAAATATC GTCCGCTACT TTGCGATGAA CTCCAGAGAG
    GAGGAGGACT CCATTGTGAT CGACATTCTG GCAGAGCACG TCAGTGGCAT CTCTCTGGCT
    ACACACCTGA GCCACTCGGG CCCAGTCCCC GCCCACCAGC TGCGCAAGTA CACAGCCCAG
    CTCCTGGCAG GCCTGGATTA CCTACACAGC AACTCCGTGG TGCACAAGGT TCTGAGCGCG
    TCCAGTGTCT TGGTAGATGC CGAGGGCACT GTCAAGATAA CAGACTACAG CATCTCTAAG
    CGTCTGGCAG ACATTTGCAA GGAGGATGTA TTTGAGCAAG CTCGAGTTCG TTTTAGTGAC
    AGTGCCCTGC CTTATAAAAC AGGAAAGAAA GGGGATGTGT GGCGGCTCGG CCTCCTGCTG
    TTGTCTCTCA GCCAAGGACA GGAGTGTGGG GAGTATCCGG TGACCATCCC CAGTGACCTG
    CCAGCTGACT TCCAAGACTT CCTGAAGAAG TGTGTCTGCC TGGACGACAA GGAAAGATGG
    AGCCCTCAGC AGCTGCTGAA ACACAGCTTT ATAAACCCTC AACCAAAGCT GCCTTTGGTG
    GAGCAGAGTC CTGAAGACTC TGGGGGACAA GACTACATTG AGACCGTCAT TCCCAGCAAC
    CAGCTGCCCA GCGCTGCGTT CTTCAGTGAG ACACAGAAAC AGTTCTCCCG GTACTTCATT
    GAGTTTGAAG AACTACAACT CCTAGGGAAA GGAGCTTTTG GAGCTGTCAT CAAGGTGCAA
    AACAAGCTTG ATGGCTGCTG CTATGCTGTG AAGCGCATCC CTATCAACCC TGCAAGCAGA
    CACTTCCGCC GGATCAAGGG CGAGGTGACA CTGCTATCGC GCCTGCACCA TGAGAACATT
    GTGCGCTACT ACAACGCCTG GATCGAGCGG CATGAGCGCC CAGCGGTGCC AGGGACACCG
    CCCCCAGACT GCACACCCCA GGCCCAGGAC AGCCCAGCCA CCTGCGGGAA AACATCAGGC
    GACACTGAAG AACTGGGCAG CGTGGAGGCT GCAGCACCGC CACCCATCCT CAGCAGCTCG
    GTGGAGTGGA GCACATCTGC AGAGCGTTCT ACCAGCACCC GCTTCCCAGT CACGGGCCAG
    GATTCCAGCA GCGATGAGGA GGACGAGGAC GAGCGCGATG GCGTCTTCTC CCAGTCCTTT
    CTACCTGCTT CAGATTCTGA CAGTGACATC ATCTTTGACA ATGAAGATGA AAACAGTAAA
    AGTCAAAATC AGGATGAAGA CTGCAATCAA AAGGACGGTA GCCATGAGAT TGAGCCTTCA
    GTGACAGCTG AGGCTGTGCA CTACCTTTAT ATCCAGATGG AGTACTGCGA GAAGAGCACG
    CTGCGGGACA CCATTGACCA GGGGCTGTTC CGAGACACCA GCCGGCTCTG GAGGCTTTTC
    CGAGAGATTC TGGATGGATT AGCTTATATC CATGAGAAAG GAATGATTCA TCGCGACTTG
    AAGCCTGTCA ATATTTTTTT GGATTCTGAT GACCATGTGA AAATAGGTGA CTTTGGCCTG
    GCAACAGACC ATCTGGCCTT CACTGCTGAA GGTAAACAGG ACGACCAGGC AGGTGACGGA
    GTGATTAAGT CAGACCCTTC AGGCCATTTG ACTGGCATGG TTGGTACTGC TCTGTATGTA
    AGCCCTGAGG TCCAAGGAAG CACCAAGTCT GCATACAACC AGAAAGTGGA TCTCTTCAGC
    CTGGGAATTA TCTTCTTTGA GATGTCTTAT CACCCGATGG TGACTGCCTC AGAAAGAATT
    TTCGTTCTCA ACCAACTTCG AGATCCCACA TCGCCCAAGT TTCCAGACGA CTTCGATGAT
    GGAGAACATA CAAAGCAGAA ATCTGTCATC TCCTGGCTGT TGAACCATGA TCCAGCTAAA
    CGGCCCACGG CCATGGAACT GCTCAAGAGT GAGCTGCTCC CCCCGCCGCA GATGGAGGAG
    TCCGAGCTGC ATGAAGTGCT GCACCACACG CTGGCCAACA TAGATGGGAA GGCCTACCGC
    ACCATGATGA GTCAGATCTT CTGCCAGCAC ATCTCCCCCG CTATAGACTA CACCTACGAC
    AGTGACATCC TGAAGGGCAA CTTCTTGATT CGTACAGCCA AGATCCAGCA GCTTGTATGT
    GAAACCATCG TCCGCGTCTT TAAAAGGCAT GGCGCTGTCC AGCTGTGCAC CCCACTGCTG
    CTTCCCCGAA ACAGGCAAAT ATATGAGCAC AACGAAGCGG CTTTGTTCAT GGATCACAGC
    GGCATGCTGG TGATGCTTCC GTTCGACCTG CGGGTCCCTT TTGCAAGATA TGTGGCAAGA
    AATAACATAC TGAATTTAAA ACGATACTGC ATAGAGCGGG TGTTCAGACC TCGGAAGTTA
    GACCGATTTC ATCCCAAAGA ACTCCTGGAA TGTGCATTTG ATATCGTCAC TTCTACCACC
    AACAGCTCTC TGCCCACTGC CGAAACCATC TACACCATCT ATGAAATAAT CCAGGAGTTT
    CCTGCACTTC AGGAAAGGAA TTACAGCATT TACTTGAACC ATACGATGCT TCTGAAGGCA
    ATACTCCTAC ACTGTGGGAT CCCAGAGGAC AAGCTCAGCC AAGTCTACGT CATTCTGTAT
    GATGCTGTGA CAGAGAAGCT GACTAGGAGA GAAGTGGAAG CTAAATTCTG TAACCTGTCC
    TTGTCTTCTA ATAGCCTGTG TAGACTCTAC AAGTTCATTG AGCAGAAGGG GGATTTGCAA
    GACTTAACGC CAACCATCAA CTCACTAATA AAACAGAAAA CAGGCGTTGC TCAGTTGGTG
    AAGTATAGCT TAAAAGACCT CGAGGACGTC GTCGGGCTGC TGAAGAAACT TGGCGTGAAG
    TTACAGGTCT CCATCAACCT GGGCTTGGTC TACAAGGTGC AGCAGCACAC TGGCATCATC
    TTCCAGTTCC TGGCATTCAG CAAACGCAGG CAAAGGGTTG TGCCTGAGAT CCTTGCCGCC
    GGTGGCAGAT ACGACCTGCT GATTCCCAAG TTCAGAGGCC CACAGACTGT GGGGCCAGTC
    CCCACTGCTG TCGGTGTCAG CATAGCCATA GACAAGATAT TTGCTGCTGT CCTCAACATG
    GAAGAGCCTG TTACAGTGAG CTCCTGTGAC CTCCTGGTTG TCAGTGTTGG CCAGATGTCC
    ATGTCCAGGG CCATCAACCT AACCCAGAAA CTGTGGACAG CGGGCATCAC TGCAGAAATC
    ATGTATGACT GGTCCCAGTC CCAGGAAGAG TTACAAGAGT ACTGCAGACA CCATGAAATC
    ACCTATGTGG CTCTGGTCTC CGATAAAGAA GGAAGCCATG TCAAGGTCAA GTCCTTTGAG
    AAGGAGAGGC AAACAGAAAA GCGTGTATTG GAATCGGATC TTGTGGATCA CGTTATGCAG
    AAACTAAGAA CCAAAGTTGG TGACGAAAGA AATTTCAGAG ACGCTTCTGA TAATCTTGCC
    GTACAAACTC TGAAGGGGTC GTTTTCTAAT GCTTCAGGTT TGTTTGAAAT CCATGGAACT
    ACAGTAGTCC CCAACGTGAT TGTTCTAGCA CCAGAAAAGC TGTCAGCCAG CACCAGGAGG
    CGACATGAGA TTCAGGTGCA AACCCGACTT CAGACTACAC TTGCCAACTT ACATCAGAAA
    AGCAGTGAAA TTGAAATTTT GGCTGTGGAC CTACCCAAGG AAACAATCTT ACAGTTTCTA
    TCATTAGAGT GGGATGCTGA TGAACAGGCA TTTAACACAA CTGTGAAGCA GCTGCTGTCA
    CGCCTGCCAA AGCAAAGATA CCTCAAACTC GTCTGCGATG AAATTTATAA CATCAAAGTT
    GAAAAGAAGG TGTCAGTGCT GTTCCTGTAC AGCTACAGAG ATGACTACTA CAGAATCCTA
    TTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu17698
    Clone ID Related Accession (Same CDS sequence) NM_013719.3
    Accession Version NM_013719.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-09
    Organism Mus musculus(house mouse)
    Product eIF-2-alpha kinase GCN2 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL929163.5, AK013758.1, CO430666.1, CN527645.1, BG086385.2, CB723221.1, CF748039.1, BF453236.1, BF462783.1, W75391.1, BM250244.2 and AI324072.1. On Apr 28, 2010 this sequence version replaced NM_013719.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ243533.1, AF193343.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCTGGGG GCCGCGGGGC CTCTGGACGC GGCCGGGCTG AGCCACAGGA GAGCTATTCG 
    CAGCGACAGG ACCACGAGCT GCAGGCCCTG GAGGCCATTT ACGGCTCGGA CTTCCAGGAT
    CTGCGGCCGG ATGCGCGCGG ACGGGTGAGA GAGCCTCCTG AAATCAACTT AGTTCTTTAC
    CCTCAGGGCC TAGCTGGTGA AGAAGTATAC GTGCAAGTGG AACTGAGGGT TAAATGCCCA
    CCTACATACC CAGATGTAGT TCCCGAAATA GACTTAAAAA ATGCTAAAGG CCTGTCGAAT
    GAAAGTGTTA ATTTGTTAAA ATCACACCTG GAAGAACTGG CCAAAAAGCA GTGTGGAGAG
    GTGATGATAT TTGAACTGGC ACACCATGTG CAGTCGTTTC TCAGCGAGCA TAACAAGCCC
    CCACCAAAGT CTTTCCATGA AGAAATGCTG GAAAGGCAGG CTCAGGAGAA GCAGCAGAGG
    TTGCTGGAGG CCAGGCGGAA GGAGGAGCAG GAGCAACGTG AAATCCTCCA CGAGATTCAG
    AGAAGGAAAG AGGAGATCAA GGAAGAGAAG AAAAGGAAGG AAATGGCTAA GCAGGAACGT
    TTGGAAATCA CTAGCTTGAC AAACCAGGAC TATGCCTCTA AGAGAGACCC AGCAGGGCAC
    AGGGCAGCTG CCATCCTCCA TGGAGGCTCT CCTGACTTTG TAGGAAATGG TAAAGCCCGG
    ACATACTCCT CAGGAAGGTC CAGGCGAGAA CGTCAGTATT CTGTGTGTAG TGGTGAACCC
    TCTCCTGGCT CGTGTGACAT CCTCCATTTC TCTGTGGGTA GCCCTGATCA GCTCATGGTG
    CACAAAGGGA GATGTGTTGG CAGTGATGAG CAGCTGGGAA AGGTGGTATA CAATGCTTTG
    GAAACAGCCA CGGGCAGCTT TGTCTTGCTG CATGAGTGGG TCCTTCAATG GCAGAAGATG
    GGCCCTTGCC TTACCAGTCA AGAGAAAGAG AAGATTGACA AGTGCAAAAG GCAGATTCAA
    GGAGCAGAAA CAGAATTCAG CTCCCTAGTG AAACTGAGCC ATCCAAATAT CGTCCGCTAC
    TTTGCGATGA ACTCCAGAGA GGAGGAGGAC TCCATTGTGA TCGACATTCT GGCAGAGCAC
    GTCAGTGGCA TCTCTCTGGC TACACACCTG AGCCACTCGG GCCCAGTCCC CGCCCACCAG
    CTGCGCAAGT ACACAGCCCA GCTCCTGGCA GGCCTGGATT ACCTACACAG CAACTCCGTG
    GTGCACAAGG TTCTGAGCGC GTCCAGTGTC TTGGTAGATG CCGAGGGCAC TGTCAAGATA
    ACAGACTACA GCATCTCTAA GCGTCTGGCA GACATTTGCA AGGAGGATGT ATTTGAGCAA
    GCTCGAGTTC GTTTTAGTGA CAGTGCCCTG CCTTATAAAA CAGGAAAGAA AGGGGATGTG
    TGGCGGCTCG GCCTCCTGCT GTTGTCTCTC AGCCAAGGAC AGGAGTGTGG GGAGTATCCG
    GTGACCATCC CCAGTGACCT GCCAGCTGAC TTCCAAGACT TCCTGAAGAA GTGTGTCTGC
    CTGGACGACA AGGAAAGATG GAGCCCTCAG CAGCTGCTGA AACACAGCTT TATAAACCCT
    CAACCAAAGC TGCCTTTGGT GGAGCAGAGT CCTGAAGACT CTGGGGGACA AGACTACATT
    GAGACCGTCA TTCCCAGCAA CCAGCTGCCC AGCGCTGCGT TCTTCAGTGA GACACAGAAA
    CAGTTCTCCC GGTACTTCAT TGAGTTTGAA GAACTACAAC TCCTAGGGAA AGGAGCTTTT
    GGAGCTGTCA TCAAGGTGCA AAACAAGCTT GATGGCTGCT GCTATGCTGT GAAGCGCATC
    CCTATCAACC CTGCAAGCAG ACACTTCCGC CGGATCAAGG GCGAGGTGAC ACTGCTATCG
    CGCCTGCACC ATGAGAACAT TGTGCGCTAC TACAACGCCT GGATCGAGCG GCATGAGCGC
    CCAGCGGTGC CAGGGACACC GCCCCCAGAC TGCACACCCC AGGCCCAGGA CAGCCCAGCC
    ACCTGCGGGA AAACATCAGG CGACACTGAA GAACTGGGCA GCGTGGAGGC TGCAGCACCG
    CCACCCATCC TCAGCAGCTC GGTGGAGTGG AGCACATCTG CAGAGCGTTC TACCAGCACC
    CGCTTCCCAG TCACGGGCCA GGATTCCAGC AGCGATGAGG AGGACGAGGA CGAGCGCGAT
    GGCGTCTTCT CCCAGTCCTT TCTACCTGCT TCAGATTCTG ACAGTGACAT CATCTTTGAC
    AATGAAGATG AAAACAGTAA AAGTCAAAAT CAGGATGAAG ACTGCAATCA AAAGGACGGT
    AGCCATGAGA TTGAGCCTTC AGTGACAGCT GAGGCTGTGC ACTACCTTTA TATCCAGATG
    GAGTACTGCG AGAAGAGCAC GCTGCGGGAC ACCATTGACC AGGGGCTGTT CCGAGACACC
    AGCCGGCTCT GGAGGCTTTT CCGAGAGATT CTGGATGGAT TAGCTTATAT CCATGAGAAA
    GGAATGATTC ATCGCGACTT GAAGCCTGTC AATATTTTTT TGGATTCTGA TGACCATGTG
    AAAATAGGTG ACTTTGGCCT GGCAACAGAC CATCTGGCCT TCACTGCTGA AGGTAAACAG
    GACGACCAGG CAGGTGACGG AGTGATTAAG TCAGACCCTT CAGGCCATTT GACTGGCATG
    GTTGGTACTG CTCTGTATGT AAGCCCTGAG GTCCAAGGAA GCACCAAGTC TGCATACAAC
    CAGAAAGTGG ATCTCTTCAG CCTGGGAATT ATCTTCTTTG AGATGTCTTA TCACCCGATG
    GTGACTGCCT CAGAAAGAAT TTTCGTTCTC AACCAACTTC GAGATCCCAC ATCGCCCAAG
    TTTCCAGACG ACTTCGATGA TGGAGAACAT ACAAAGCAGA AATCTGTCAT CTCCTGGCTG
    TTGAACCATG ATCCAGCTAA ACGGCCCACG GCCATGGAAC TGCTCAAGAG TGAGCTGCTC
    CCCCCGCCGC AGATGGAGGA GTCCGAGCTG CATGAAGTGC TGCACCACAC GCTGGCCAAC
    ATAGATGGGA AGGCCTACCG CACCATGATG AGTCAGATCT TCTGCCAGCA CATCTCCCCC
    GCTATAGACT ACACCTACGA CAGTGACATC CTGAAGGGCA ACTTCTTGAT TCGTACAGCC
    AAGATCCAGC AGCTTGTATG TGAAACCATC GTCCGCGTCT TTAAAAGGCA TGGCGCTGTC
    CAGCTGTGCA CCCCACTGCT GCTTCCCCGA AACAGGCAAA TATATGAGCA CAACGAAGCG
    GCTTTGTTCA TGGATCACAG CGGCATGCTG GTGATGCTTC CGTTCGACCT GCGGGTCCCT
    TTTGCAAGAT ATGTGGCAAG AAATAACATA CTGAATTTAA AACGATACTG CATAGAGCGG
    GTGTTCAGAC CTCGGAAGTT AGACCGATTT CATCCCAAAG AACTCCTGGA ATGTGCATTT
    GATATCGTCA CTTCTACCAC CAACAGCTCT CTGCCCACTG CCGAAACCAT CTACACCATC
    TATGAAATAA TCCAGGAGTT TCCTGCACTT CAGGAAAGGA ATTACAGCAT TTACTTGAAC
    CATACGATGC TTCTGAAGGC AATACTCCTA CACTGTGGGA TCCCAGAGGA CAAGCTCAGC
    CAAGTCTACG TCATTCTGTA TGATGCTGTG ACAGAGAAGC TGACTAGGAG AGAAGTGGAA
    GCTAAATTCT GTAACCTGTC CTTGTCTTCT AATAGCCTGT GTAGACTCTA CAAGTTCATT
    GAGCAGAAGG GGGATTTGCA AGACTTAACG CCAACCATCA ACTCACTAAT AAAACAGAAA
    ACAGGCGTTG CTCAGTTGGT GAAGTATAGC TTAAAAGACC TCGAGGACGT CGTCGGGCTG
    CTGAAGAAAC TTGGCGTGAA GTTACAGGTC TCCATCAACC TGGGCTTGGT CTACAAGGTG
    CAGCAGCACA CTGGCATCAT CTTCCAGTTC CTGGCATTCA GCAAACGCAG GCAAAGGGTT
    GTGCCTGAGA TCCTTGCCGC CGGTGGCAGA TACGACCTGC TGATTCCCAA GTTCAGAGGC
    CCACAGACTG TGGGGCCAGT CCCCACTGCT GTCGGTGTCA GCATAGCCAT AGACAAGATA
    TTTGCTGCTG TCCTCAACAT GGAAGAGCCT GTTACAGTGA GCTCCTGTGA CCTCCTGGTT
    GTCAGTGTTG GCCAGATGTC CATGTCCAGG GCCATCAACC TAACCCAGAA ACTGTGGACA
    GCGGGCATCA CTGCAGAAAT CATGTATGAC TGGTCCCAGT CCCAGGAAGA GTTACAAGAG
    TACTGCAGAC ACCATGAAAT CACCTATGTG GCTCTGGTCT CCGATAAAGA AGGAAGCCAT
    GTCAAGGTCA AGTCCTTTGA GAAGGAGAGG CAAACAGAAA AGCGTGTATT GGAATCGGAT
    CTTGTGGATC ACGTTATGCA GAAACTAAGA ACCAAAGTTG GTGACGAAAG AAATTTCAGA
    GACGCTTCTG ATAATCTTGC CGTACAAACT CTGAAGGGGT CGTTTTCTAA TGCTTCAGGT
    TTGTTTGAAA TCCATGGAAC TACAGTAGTC CCCAACGTGA TTGTTCTAGC ACCAGAAAAG
    CTGTCAGCCA GCACCAGGAG GCGACATGAG ATTCAGGTGC AAACCCGACT TCAGACTACA
    CTTGCCAACT TACATCAGAA AAGCAGTGAA ATTGAAATTT TGGCTGTGGA CCTACCCAAG
    GAAACAATCT TACAGTTTCT ATCATTAGAG TGGGATGCTG ATGAACAGGC ATTTAACACA
    ACTGTGAAGC AGCTGCTGTC ACGCCTGCCA AAGCAAAGAT ACCTCAAACT CGTCTGCGAT
    GAAATTTATA ACATCAAAGT TGAAAAGAAG GTGTCAGTGC TGTTCCTGTA CAGCTACAGA
    GATGACTACT ACAGAATCCT ATTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_038747.2
    CDS93..5039
    Translation

    Target ORF information:

    RefSeq Version NM_013719.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_013719.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCTGGGG GCCGCGGGGC CTCTGGACGC GGCCGGGCTG AGCCACAGGA GAGCTATTCG 
    CAGCGACAGG ACCACGAGCT GCAGGCCCTG GAGGCCATTT ACGGCTCGGA CTTCCAGGAT
    CTGCGGCCGG ATGCGCGCGG ACGGGTGAGA GAGCCTCCTG AAATCAACTT AGTTCTTTAC
    CCTCAGGGCC TAGCTGGTGA AGAAGTATAC GTGCAAGTGG AACTGAGGGT TAAATGCCCA
    CCTACATACC CAGATGTAGT TCCCGAAATA GACTTAAAAA ATGCTAAAGG CCTGTCGAAT
    GAAAGTGTTA ATTTGTTAAA ATCACACCTG GAAGAACTGG CCAAAAAGCA GTGTGGAGAG
    GTGATGATAT TTGAACTGGC ACACCATGTG CAGTCGTTTC TCAGCGAGCA TAACAAGCCC
    CCACCAAAGT CTTTCCATGA AGAAATGCTG GAAAGGCAGG CTCAGGAGAA GCAGCAGAGG
    TTGCTGGAGG CCAGGCGGAA GGAGGAGCAG GAGCAACGTG AAATCCTCCA CGAGATTCAG
    AGAAGGAAAG AGGAGATCAA GGAAGAGAAG AAAAGGAAGG AAATGGCTAA GCAGGAACGT
    TTGGAAATCA CTAGCTTGAC AAACCAGGAC TATGCCTCTA AGAGAGACCC AGCAGGGCAC
    AGGGCAGCTG CCATCCTCCA TGGAGGCTCT CCTGACTTTG TAGGAAATGG TAAAGCCCGG
    ACATACTCCT CAGGAAGGTC CAGGCGAGAA CGTCAGTATT CTGTGTGTAG TGGTGAACCC
    TCTCCTGGCT CGTGTGACAT CCTCCATTTC TCTGTGGGTA GCCCTGATCA GCTCATGGTG
    CACAAAGGGA GATGTGTTGG CAGTGATGAG CAGCTGGGAA AGGTGGTATA CAATGCTTTG
    GAAACAGCCA CGGGCAGCTT TGTCTTGCTG CATGAGTGGG TCCTTCAATG GCAGAAGATG
    GGCCCTTGCC TTACCAGTCA AGAGAAAGAG AAGATTGACA AGTGCAAAAG GCAGATTCAA
    GGAGCAGAAA CAGAATTCAG CTCCCTAGTG AAACTGAGCC ATCCAAATAT CGTCCGCTAC
    TTTGCGATGA ACTCCAGAGA GGAGGAGGAC TCCATTGTGA TCGACATTCT GGCAGAGCAC
    GTCAGTGGCA TCTCTCTGGC TACACACCTG AGCCACTCGG GCCCAGTCCC CGCCCACCAG
    CTGCGCAAGT ACACAGCCCA GCTCCTGGCA GGCCTGGATT ACCTACACAG CAACTCCGTG
    GTGCACAAGG TTCTGAGCGC GTCCAGTGTC TTGGTAGATG CCGAGGGCAC TGTCAAGATA
    ACAGACTACA GCATCTCTAA GCGTCTGGCA GACATTTGCA AGGAGGATGT ATTTGAGCAA
    GCTCGAGTTC GTTTTAGTGA CAGTGCCCTG CCTTATAAAA CAGGAAAGAA AGGGGATGTG
    TGGCGGCTCG GCCTCCTGCT GTTGTCTCTC AGCCAAGGAC AGGAGTGTGG GGAGTATCCG
    GTGACCATCC CCAGTGACCT GCCAGCTGAC TTCCAAGACT TCCTGAAGAA GTGTGTCTGC
    CTGGACGACA AGGAAAGATG GAGCCCTCAG CAGCTGCTGA AACACAGCTT TATAAACCCT
    CAACCAAAGC TGCCTTTGGT GGAGCAGAGT CCTGAAGACT CTGGGGGACA AGACTACATT
    GAGACCGTCA TTCCCAGCAA CCAGCTGCCC AGCGCTGCGT TCTTCAGTGA GACACAGAAA
    CAGTTCTCCC GGTACTTCAT TGAGTTTGAA GAACTACAAC TCCTAGGGAA AGGAGCTTTT
    GGAGCTGTCA TCAAGGTGCA AAACAAGCTT GATGGCTGCT GCTATGCTGT GAAGCGCATC
    CCTATCAACC CTGCAAGCAG ACACTTCCGC CGGATCAAGG GCGAGGTGAC ACTGCTATCG
    CGCCTGCACC ATGAGAACAT TGTGCGCTAC TACAACGCCT GGATCGAGCG GCATGAGCGC
    CCAGCGGTGC CAGGGACACC GCCCCCAGAC TGCACACCCC AGGCCCAGGA CAGCCCAGCC
    ACCTGCGGGA AAACATCAGG CGACACTGAA GAACTGGGCA GCGTGGAGGC TGCAGCACCG
    CCACCCATCC TCAGCAGCTC GGTGGAGTGG AGCACATCTG CAGAGCGTTC TACCAGCACC
    CGCTTCCCAG TCACGGGCCA GGATTCCAGC AGCGATGAGG AGGACGAGGA CGAGCGCGAT
    GGCGTCTTCT CCCAGTCCTT TCTACCTGCT TCAGATTCTG ACAGTGACAT CATCTTTGAC
    AATGAAGATG AAAACAGTAA AAGTCAAAAT CAGGATGAAG ACTGCAATCA AAAGGACGGT
    AGCCATGAGA TTGAGCCTTC AGTGACAGCT GAGGCTGTGC ACTACCTTTA TATCCAGATG
    GAGTACTGCG AGAAGAGCAC GCTGCGGGAC ACCATTGACC AGGGGCTGTT CCGAGACACC
    AGCCGGCTCT GGAGGCTTTT CCGAGAGATT CTGGATGGAT TAGCTTATAT CCATGAGAAA
    GGAATGATTC ATCGCGACTT GAAGCCTGTC AATATTTTTT TGGATTCTGA TGACCATGTG
    AAAATAGGTG ACTTTGGCCT GGCAACAGAC CATCTGGCCT TCACTGCTGA AGGTAAACAG
    GACGACCAGG CAGGTGACGG AGTGATTAAG TCAGACCCTT CAGGCCATTT GACTGGCATG
    GTTGGTACTG CTCTGTATGT AAGCCCTGAG GTCCAAGGAA GCACCAAGTC TGCATACAAC
    CAGAAAGTGG ATCTCTTCAG CCTGGGAATT ATCTTCTTTG AGATGTCTTA TCACCCGATG
    GTGACTGCCT CAGAAAGAAT TTTCGTTCTC AACCAACTTC GAGATCCCAC ATCGCCCAAG
    TTTCCAGACG ACTTCGATGA TGGAGAACAT ACAAAGCAGA AATCTGTCAT CTCCTGGCTG
    TTGAACCATG ATCCAGCTAA ACGGCCCACG GCCATGGAAC TGCTCAAGAG TGAGCTGCTC
    CCCCCGCCGC AGATGGAGGA GTCCGAGCTG CATGAAGTGC TGCACCACAC GCTGGCCAAC
    ATAGATGGGA AGGCCTACCG CACCATGATG AGTCAGATCT TCTGCCAGCA CATCTCCCCC
    GCTATAGACT ACACCTACGA CAGTGACATC CTGAAGGGCA ACTTCTTGAT TCGTACAGCC
    AAGATCCAGC AGCTTGTATG TGAAACCATC GTCCGCGTCT TTAAAAGGCA TGGCGCTGTC
    CAGCTGTGCA CCCCACTGCT GCTTCCCCGA AACAGGCAAA TATATGAGCA CAACGAAGCG
    GCTTTGTTCA TGGATCACAG CGGCATGCTG GTGATGCTTC CGTTCGACCT GCGGGTCCCT
    TTTGCAAGAT ATGTGGCAAG AAATAACATA CTGAATTTAA AACGATACTG CATAGAGCGG
    GTGTTCAGAC CTCGGAAGTT AGACCGATTT CATCCCAAAG AACTCCTGGA ATGTGCATTT
    GATATCGTCA CTTCTACCAC CAACAGCTCT CTGCCCACTG CCGAAACCAT CTACACCATC
    TATGAAATAA TCCAGGAGTT TCCTGCACTT CAGGAAAGGA ATTACAGCAT TTACTTGAAC
    CATACGATGC TTCTGAAGGC AATACTCCTA CACTGTGGGA TCCCAGAGGA CAAGCTCAGC
    CAAGTCTACG TCATTCTGTA TGATGCTGTG ACAGAGAAGC TGACTAGGAG AGAAGTGGAA
    GCTAAATTCT GTAACCTGTC CTTGTCTTCT AATAGCCTGT GTAGACTCTA CAAGTTCATT
    GAGCAGAAGG GGGATTTGCA AGACTTAACG CCAACCATCA ACTCACTAAT AAAACAGAAA
    ACAGGCGTTG CTCAGTTGGT GAAGTATAGC TTAAAAGACC TCGAGGACGT CGTCGGGCTG
    CTGAAGAAAC TTGGCGTGAA GTTACAGGTC TCCATCAACC TGGGCTTGGT CTACAAGGTG
    CAGCAGCACA CTGGCATCAT CTTCCAGTTC CTGGCATTCA GCAAACGCAG GCAAAGGGTT
    GTGCCTGAGA TCCTTGCCGC CGGTGGCAGA TACGACCTGC TGATTCCCAA GTTCAGAGGC
    CCACAGACTG TGGGGCCAGT CCCCACTGCT GTCGGTGTCA GCATAGCCAT AGACAAGATA
    TTTGCTGCTG TCCTCAACAT GGAAGAGCCT GTTACAGTGA GCTCCTGTGA CCTCCTGGTT
    GTCAGTGTTG GCCAGATGTC CATGTCCAGG GCCATCAACC TAACCCAGAA ACTGTGGACA
    GCGGGCATCA CTGCAGAAAT CATGTATGAC TGGTCCCAGT CCCAGGAAGA GTTACAAGAG
    TACTGCAGAC ACCATGAAAT CACCTATGTG GCTCTGGTCT CCGATAAAGA AGGAAGCCAT
    GTCAAGGTCA AGTCCTTTGA GAAGGAGAGG CAAACAGAAA AGCGTGTATT GGAATCGGAT
    CTTGTGGATC ACGTTATGCA GAAACTAAGA ACCAAAGTTG GTGACGAAAG AAATTTCAGA
    GACGCTTCTG ATAATCTTGC CGTACAAACT CTGAAGGGGT CGTTTTCTAA TGCTTCAGGT
    TTGTTTGAAA TCCATGGAAC TACAGTAGTC CCCAACGTGA TTGTTCTAGC ACCAGAAAAG
    CTGTCAGCCA GCACCAGGAG GCGACATGAG ATTCAGGTGC AAACCCGACT TCAGACTACA
    CTTGCCAACT TACATCAGAA AAGCAGTGAA ATTGAAATTT TGGCTGTGGA CCTACCCAAG
    GAAACAATCT TACAGTTTCT ATCATTAGAG TGGGATGCTG ATGAACAGGC ATTTAACACA
    ACTGTGAAGC AGCTGCTGTC ACGCCTGCCA AAGCAAAGAT ACCTCAAACT CGTCTGCGAT
    GAAATTTATA ACATCAAAGT TGAAAAGAAG GTGTCAGTGC TGTTCCTGTA CAGCTACAGA
    GATGACTACT ACAGAATCCT ATTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu17792
    Clone ID Related Accession (Same CDS sequence) NM_001177806.1 , XM_006499625.3 , XM_006499625.4
    Accession Version NM_001177806.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4611bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-09
    Organism Mus musculus(house mouse)
    Product eIF-2-alpha kinase GCN2 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC072637.1, AI643894.1 and AI324072.1. Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence and lacks an alternate internal exon in the 5' end compared to variant 1, that causes a frameshift. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC072637.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCCCACCT ACATACCCAG ATGTGTGATG ATATTTGAAC TGGCACACCA TGTGCAGTCG 
    TTTCTCAGCG AGCATAACAA GCCCCCACCA AAGTCTTTCC ATGAAGAAAT GCTGGAAAGG
    CAGGCTCAGG AGAAGCAGCA GAGGTTGCTG GAGGCCAGGC GGAAGGAGGA GCAGGAGCAA
    CGTGAAATCC TCCACGAGAT TCAGAGAAGG AAAGAGGAGA TCAAGGAAGA GAAGAAAAGG
    AAGGAAATGG CTAAGCAGGA ACGTTTGGAA ATCACTAGCT TGACAAACCA GGACTATGCC
    TCTAAGAGAG ACCCAGCAGG GCACAGGGCA GCTGCCATCC TCCATGGAGG CTCTCCTGAC
    TTTGTAGGAA ATGGTAAAGC CCGGACATAC TCCTCAGGAA GGTCCAGGCG AGAACGTCAG
    TATTCTGTGT GTAGTGGTGA ACCCTCTCCT GGCTCGTGTG ACATCCTCCA TTTCTCTGTG
    GGTAGCCCTG ATCAGCTCAT GGTGCACAAA GGGAGATGTG TTGGCAGTGA TGAGCAGCTG
    GGAAAGGTGG TATACAATGC TTTGGAAACA GCCACGGGCA GCTTTGTCTT GCTGCATGAG
    TGGGTCCTTC AATGGCAGAA GATGGGCCCT TGCCTTACCA GTCAAGAGAA AGAGAAGATT
    GACAAGTGCA AAAGGCAGAT TCAAGGAGCA GAAACAGAAT TCAGCTCCCT AGTGAAACTG
    AGCCATCCAA ATATCGTCCG CTACTTTGCG ATGAACTCCA GAGAGGAGGA GGACTCCATT
    GTGATCGACA TTCTGGCAGA GCACGTCAGT GGCATCTCTC TGGCTACACA CCTGAGCCAC
    TCGGGCCCAG TCCCCGCCCA CCAGCTGCGC AAGTACACAG CCCAGCTCCT GGCAGGCCTG
    GATTACCTAC ACAGCAACTC CGTGGTGCAC AAGGTTCTGA GCGCGTCCAG TGTCTTGGTA
    GATGCCGAGG GCACTGTCAA GATAACAGAC TACAGCATCT CTAAGCGTCT GGCAGACATT
    TGCAAGGAGG ATGTATTTGA GCAAGCTCGA GTTCGTTTTA GTGACAGTGC CCTGCCTTAT
    AAAACAGGAA AGAAAGGGGA TGTGTGGCGG CTCGGCCTCC TGCTGTTGTC TCTCAGCCAA
    GGACAGGAGT GTGGGGAGTA TCCGGTGACC ATCCCCAGTG ACCTGCCAGC TGACTTCCAA
    GACTTCCTGA AGAAGTGTGT CTGCCTGGAC GACAAGGAAA GATGGAGCCC TCAGCAGCTG
    CTGAAACACA GCTTTATAAA CCCTCAACCA AAGCTGCCTT TGGTGGAGCA GAGTCCTGAA
    GACTCTGGGG GACAAGACTA CATTGAGACC GTCATTCCCA GCAACCAGCT GCCCAGCGCT
    GCGTTCTTCA GTGAGACACA GAAACAGTTC TCCCGGTACT TCATTGAGTT TGAAGAACTA
    CAACTCCTAG GGAAAGGAGC TTTTGGAGCT GTCATCAAGG TGCAAAACAA GCTTGATGGC
    TGCTGCTATG CTGTGAAGCG CATCCCTATC AACCCTGCAA GCAGACACTT CCGCCGGATC
    AAGGGCGAGG TGACACTGCT ATCGCGCCTG CACCATGAGA ACATTGTGCG CTACTACAAC
    GCCTGGATCG AGCGGCATGA GCGCCCAGCG GTGCCAGGGA CACCGCCCCC AGACTGCACA
    CCCCAGGCCC AGGACAGCCC AGCCACCTGC GGGAAAACAT CAGGCGACAC TGAAGAACTG
    GGCAGCGTGG AGGCTGCAGC ACCGCCACCC ATCCTCAGCA GCTCGGTGGA GTGGAGCACA
    TCTGCAGAGC GTTCTACCAG CACCCGCTTC CCAGTCACGG GCCAGGATTC CAGCAGCGAT
    GAGGAGGACG AGGACGAGCG CGATGGCGTC TTCTCCCAGT CCTTTCTACC TGCTTCAGAT
    TCTGACAGTG ACATCATCTT TGACAATGAA GATGAAAACA GTAAAAGTCA AAATCAGGAT
    GAAGACTGCA ATCAAAAGGA CGGTAGCCAT GAGATTGAGC CTTCAGTGAC AGCTGAGGCT
    GTGCACTACC TTTATATCCA GATGGAGTAC TGCGAGAAGA GCACGCTGCG GGACACCATT
    GACCAGGGGC TGTTCCGAGA CACCAGCCGG CTCTGGAGGC TTTTCCGAGA GATTCTGGAT
    GGATTAGCTT ATATCCATGA GAAAGGAATG ATTCATCGCG ACTTGAAGCC TGTCAATATT
    TTTTTGGATT CTGATGACCA TGTGAAAATA GGTGACTTTG GCCTGGCAAC AGACCATCTG
    GCCTTCACTG CTGAAGGTAA ACAGGACGAC CAGGCAGGTG ACGGAGTGAT TAAGTCAGAC
    CCTTCAGGCC ATTTGACTGG CATGGTTGGT ACTGCTCTGT ATGTAAGCCC TGAGGTCCAA
    GGAAGCACCA AGTCTGCATA CAACCAGAAA GTGGATCTCT TCAGCCTGGG AATTATCTTC
    TTTGAGATGT CTTATCACCC GATGGTGACT GCCTCAGAAA GAATTTTCGT TCTCAACCAA
    CTTCGAGATC CCACATCGCC CAAGTTTCCA GACGACTTCG ATGATGGAGA ACATACAAAG
    CAGAAATCTG TCATCTCCTG GCTGTTGAAC CATGATCCAG CTAAACGGCC CACGGCCATG
    GAACTGCTCA AGAGTGAGCT GCTCCCCCCG CCGCAGATGG AGGAGTCCGA GCTGCATGAA
    GTGCTGCACC ACACGCTGGC CAACATAGAT GGGAAGGCCT ACCGCACCAT GATGAGTCAG
    ATCTTCTGCC AGCACATCTC CCCCGCTATA GACTACACCT ACGACAGTGA CATCCTGAAG
    GGCAACTTCT TGATTCGTAC AGCCAAGATC CAGCAGCTTG TATGTGAAAC CATCGTCCGC
    GTCTTTAAAA GGCATGGCGC TGTCCAGCTG TGCACCCCAC TGCTGCTTCC CCGAAACAGG
    CAAATATATG AGCACAACGA AGCGGCTTTG TTCATGGATC ACAGCGGCAT GCTGGTGATG
    CTTCCGTTCG ACCTGCGGGT CCCTTTTGCA AGATATGTGG CAAGAAATAA CATACTGAAT
    TTAAAACGAT ACTGCATAGA GCGGGTGTTC AGACCTCGGA AGTTAGACCG ATTTCATCCC
    AAAGAACTCC TGGAATGTGC ATTTGATATC GTCACTTCTA CCACCAACAG CTCTCTGCCC
    ACTGCCGAAA CCATCTACAC CATCTATGAA ATAATCCAGG AGTTTCCTGC ACTTCAGGAA
    AGGAATTACA GCATTTACTT GAACCATACG ATGCTTCTGA AGGCAATACT CCTACACTGT
    GGGATCCCAG AGGACAAGCT CAGCCAAGTC TACGTCATTC TGTATGATGC TGTGACAGAG
    AAGCTGACTA GGAGAGAAGT GGAAGCTAAA TTCTGTAACC TGTCCTTGTC TTCTAATAGC
    CTGTGTAGAC TCTACAAGTT CATTGAGCAG AAGGGGGATT TGCAAGACTT AACGCCAACC
    ATCAACTCAC TAATAAAACA GAAAACAGGC GTTGCTCAGT TGGTGAAGTA TAGCTTAAAA
    GACCTCGAGG ACGTCGTCGG GCTGCTGAAG AAACTTGGCG TGAAGTTACA GGTCTCCATC
    AACCTGGGCT TGGTCTACAA GGTGCAGCAG CACACTGGCA TCATCTTCCA GTTCCTGGCA
    TTCAGCAAAC GCAGGCAAAG GGTTGTGCCT GAGATCCTTG CCGCCGGTGG CAGATACGAC
    CTGCTGATTC CCAAGTTCAG AGGCCCACAG ACTGTGGGGC CAGTCCCCAC TGCTGTCGGT
    GTCAGCATAG CCATAGACAA GATATTTGCT GCTGTCCTCA ACATGGAAGA GCCTGTTACA
    GTGAGCTCCT GTGACCTCCT GGTTGTCAGT GTTGGCCAGA TGTCCATGTC CAGGGCCATC
    AACCTAACCC AGAAACTGTG GACAGCGGGC ATCACTGCAG AAATCATGTA TGACTGGTCC
    CAGTCCCAGG AAGAGTTACA AGAGTACTGC AGACACCATG AAATCACCTA TGTGGCTCTG
    GTCTCCGATA AAGAAGGAAG CCATGTCAAG GTCAAGTCCT TTGAGAAGGA GAGGCAAACA
    GAAAAGCGTG TATTGGAATC GGATCTTGTG GATCACGTTA TGCAGAAACT AAGAACCAAA
    GTTGGTGACG AAAGAAATTT CAGAGACGCT TCTGATAATC TTGCCGTACA AACTCTGAAG
    GGGTCGTTTT CTAATGCTTC AGGTTTGTTT GAAATCCATG GAACTACAGT AGTCCCCAAC
    GTGATTGTTC TAGCACCAGA AAAGCTGTCA GCCAGCACCA GGAGGCGACA TGAGATTCAG
    GTGCAAACCC GACTTCAGAC TACACTTGCC AACTTACATC AGAAAAGCAG TGAAATTGAA
    ATTTTGGCTG TGGACCTACC CAAGGAAACA ATCTTACAGT TTCTATCATT AGAGTGGGAT
    GCTGATGAAC AGGCATTTAA CACAACTGTG AAGCAGCTGC TGTCACGCCT GCCAAAGCAA
    AGATACCTCA AACTCGTCTG CGATGAAATT TATAACATCA AAGTTGAAAA GAAGGTGTCA
    GTGCTGTTCC TGTACAGCTA CAGAGATGAC TACTACAGAA TCCTATTTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001171277.1
    CDS125..4735
    Translation

    Target ORF information:

    RefSeq Version NM_001177806.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001177806.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCCCACCT ACATACCCAG ATGTGTGATG ATATTTGAAC TGGCACACCA TGTGCAGTCG 
    TTTCTCAGCG AGCATAACAA GCCCCCACCA AAGTCTTTCC ATGAAGAAAT GCTGGAAAGG
    CAGGCTCAGG AGAAGCAGCA GAGGTTGCTG GAGGCCAGGC GGAAGGAGGA GCAGGAGCAA
    CGTGAAATCC TCCACGAGAT TCAGAGAAGG AAAGAGGAGA TCAAGGAAGA GAAGAAAAGG
    AAGGAAATGG CTAAGCAGGA ACGTTTGGAA ATCACTAGCT TGACAAACCA GGACTATGCC
    TCTAAGAGAG ACCCAGCAGG GCACAGGGCA GCTGCCATCC TCCATGGAGG CTCTCCTGAC
    TTTGTAGGAA ATGGTAAAGC CCGGACATAC TCCTCAGGAA GGTCCAGGCG AGAACGTCAG
    TATTCTGTGT GTAGTGGTGA ACCCTCTCCT GGCTCGTGTG ACATCCTCCA TTTCTCTGTG
    GGTAGCCCTG ATCAGCTCAT GGTGCACAAA GGGAGATGTG TTGGCAGTGA TGAGCAGCTG
    GGAAAGGTGG TATACAATGC TTTGGAAACA GCCACGGGCA GCTTTGTCTT GCTGCATGAG
    TGGGTCCTTC AATGGCAGAA GATGGGCCCT TGCCTTACCA GTCAAGAGAA AGAGAAGATT
    GACAAGTGCA AAAGGCAGAT TCAAGGAGCA GAAACAGAAT TCAGCTCCCT AGTGAAACTG
    AGCCATCCAA ATATCGTCCG CTACTTTGCG ATGAACTCCA GAGAGGAGGA GGACTCCATT
    GTGATCGACA TTCTGGCAGA GCACGTCAGT GGCATCTCTC TGGCTACACA CCTGAGCCAC
    TCGGGCCCAG TCCCCGCCCA CCAGCTGCGC AAGTACACAG CCCAGCTCCT GGCAGGCCTG
    GATTACCTAC ACAGCAACTC CGTGGTGCAC AAGGTTCTGA GCGCGTCCAG TGTCTTGGTA
    GATGCCGAGG GCACTGTCAA GATAACAGAC TACAGCATCT CTAAGCGTCT GGCAGACATT
    TGCAAGGAGG ATGTATTTGA GCAAGCTCGA GTTCGTTTTA GTGACAGTGC CCTGCCTTAT
    AAAACAGGAA AGAAAGGGGA TGTGTGGCGG CTCGGCCTCC TGCTGTTGTC TCTCAGCCAA
    GGACAGGAGT GTGGGGAGTA TCCGGTGACC ATCCCCAGTG ACCTGCCAGC TGACTTCCAA
    GACTTCCTGA AGAAGTGTGT CTGCCTGGAC GACAAGGAAA GATGGAGCCC TCAGCAGCTG
    CTGAAACACA GCTTTATAAA CCCTCAACCA AAGCTGCCTT TGGTGGAGCA GAGTCCTGAA
    GACTCTGGGG GACAAGACTA CATTGAGACC GTCATTCCCA GCAACCAGCT GCCCAGCGCT
    GCGTTCTTCA GTGAGACACA GAAACAGTTC TCCCGGTACT TCATTGAGTT TGAAGAACTA
    CAACTCCTAG GGAAAGGAGC TTTTGGAGCT GTCATCAAGG TGCAAAACAA GCTTGATGGC
    TGCTGCTATG CTGTGAAGCG CATCCCTATC AACCCTGCAA GCAGACACTT CCGCCGGATC
    AAGGGCGAGG TGACACTGCT ATCGCGCCTG CACCATGAGA ACATTGTGCG CTACTACAAC
    GCCTGGATCG AGCGGCATGA GCGCCCAGCG GTGCCAGGGA CACCGCCCCC AGACTGCACA
    CCCCAGGCCC AGGACAGCCC AGCCACCTGC GGGAAAACAT CAGGCGACAC TGAAGAACTG
    GGCAGCGTGG AGGCTGCAGC ACCGCCACCC ATCCTCAGCA GCTCGGTGGA GTGGAGCACA
    TCTGCAGAGC GTTCTACCAG CACCCGCTTC CCAGTCACGG GCCAGGATTC CAGCAGCGAT
    GAGGAGGACG AGGACGAGCG CGATGGCGTC TTCTCCCAGT CCTTTCTACC TGCTTCAGAT
    TCTGACAGTG ACATCATCTT TGACAATGAA GATGAAAACA GTAAAAGTCA AAATCAGGAT
    GAAGACTGCA ATCAAAAGGA CGGTAGCCAT GAGATTGAGC CTTCAGTGAC AGCTGAGGCT
    GTGCACTACC TTTATATCCA GATGGAGTAC TGCGAGAAGA GCACGCTGCG GGACACCATT
    GACCAGGGGC TGTTCCGAGA CACCAGCCGG CTCTGGAGGC TTTTCCGAGA GATTCTGGAT
    GGATTAGCTT ATATCCATGA GAAAGGAATG ATTCATCGCG ACTTGAAGCC TGTCAATATT
    TTTTTGGATT CTGATGACCA TGTGAAAATA GGTGACTTTG GCCTGGCAAC AGACCATCTG
    GCCTTCACTG CTGAAGGTAA ACAGGACGAC CAGGCAGGTG ACGGAGTGAT TAAGTCAGAC
    CCTTCAGGCC ATTTGACTGG CATGGTTGGT ACTGCTCTGT ATGTAAGCCC TGAGGTCCAA
    GGAAGCACCA AGTCTGCATA CAACCAGAAA GTGGATCTCT TCAGCCTGGG AATTATCTTC
    TTTGAGATGT CTTATCACCC GATGGTGACT GCCTCAGAAA GAATTTTCGT TCTCAACCAA
    CTTCGAGATC CCACATCGCC CAAGTTTCCA GACGACTTCG ATGATGGAGA ACATACAAAG
    CAGAAATCTG TCATCTCCTG GCTGTTGAAC CATGATCCAG CTAAACGGCC CACGGCCATG
    GAACTGCTCA AGAGTGAGCT GCTCCCCCCG CCGCAGATGG AGGAGTCCGA GCTGCATGAA
    GTGCTGCACC ACACGCTGGC CAACATAGAT GGGAAGGCCT ACCGCACCAT GATGAGTCAG
    ATCTTCTGCC AGCACATCTC CCCCGCTATA GACTACACCT ACGACAGTGA CATCCTGAAG
    GGCAACTTCT TGATTCGTAC AGCCAAGATC CAGCAGCTTG TATGTGAAAC CATCGTCCGC
    GTCTTTAAAA GGCATGGCGC TGTCCAGCTG TGCACCCCAC TGCTGCTTCC CCGAAACAGG
    CAAATATATG AGCACAACGA AGCGGCTTTG TTCATGGATC ACAGCGGCAT GCTGGTGATG
    CTTCCGTTCG ACCTGCGGGT CCCTTTTGCA AGATATGTGG CAAGAAATAA CATACTGAAT
    TTAAAACGAT ACTGCATAGA GCGGGTGTTC AGACCTCGGA AGTTAGACCG ATTTCATCCC
    AAAGAACTCC TGGAATGTGC ATTTGATATC GTCACTTCTA CCACCAACAG CTCTCTGCCC
    ACTGCCGAAA CCATCTACAC CATCTATGAA ATAATCCAGG AGTTTCCTGC ACTTCAGGAA
    AGGAATTACA GCATTTACTT GAACCATACG ATGCTTCTGA AGGCAATACT CCTACACTGT
    GGGATCCCAG AGGACAAGCT CAGCCAAGTC TACGTCATTC TGTATGATGC TGTGACAGAG
    AAGCTGACTA GGAGAGAAGT GGAAGCTAAA TTCTGTAACC TGTCCTTGTC TTCTAATAGC
    CTGTGTAGAC TCTACAAGTT CATTGAGCAG AAGGGGGATT TGCAAGACTT AACGCCAACC
    ATCAACTCAC TAATAAAACA GAAAACAGGC GTTGCTCAGT TGGTGAAGTA TAGCTTAAAA
    GACCTCGAGG ACGTCGTCGG GCTGCTGAAG AAACTTGGCG TGAAGTTACA GGTCTCCATC
    AACCTGGGCT TGGTCTACAA GGTGCAGCAG CACACTGGCA TCATCTTCCA GTTCCTGGCA
    TTCAGCAAAC GCAGGCAAAG GGTTGTGCCT GAGATCCTTG CCGCCGGTGG CAGATACGAC
    CTGCTGATTC CCAAGTTCAG AGGCCCACAG ACTGTGGGGC CAGTCCCCAC TGCTGTCGGT
    GTCAGCATAG CCATAGACAA GATATTTGCT GCTGTCCTCA ACATGGAAGA GCCTGTTACA
    GTGAGCTCCT GTGACCTCCT GGTTGTCAGT GTTGGCCAGA TGTCCATGTC CAGGGCCATC
    AACCTAACCC AGAAACTGTG GACAGCGGGC ATCACTGCAG AAATCATGTA TGACTGGTCC
    CAGTCCCAGG AAGAGTTACA AGAGTACTGC AGACACCATG AAATCACCTA TGTGGCTCTG
    GTCTCCGATA AAGAAGGAAG CCATGTCAAG GTCAAGTCCT TTGAGAAGGA GAGGCAAACA
    GAAAAGCGTG TATTGGAATC GGATCTTGTG GATCACGTTA TGCAGAAACT AAGAACCAAA
    GTTGGTGACG AAAGAAATTT CAGAGACGCT TCTGATAATC TTGCCGTACA AACTCTGAAG
    GGGTCGTTTT CTAATGCTTC AGGTTTGTTT GAAATCCATG GAACTACAGT AGTCCCCAAC
    GTGATTGTTC TAGCACCAGA AAAGCTGTCA GCCAGCACCA GGAGGCGACA TGAGATTCAG
    GTGCAAACCC GACTTCAGAC TACACTTGCC AACTTACATC AGAAAAGCAG TGAAATTGAA
    ATTTTGGCTG TGGACCTACC CAAGGAAACA ATCTTACAGT TTCTATCATT AGAGTGGGAT
    GCTGATGAAC AGGCATTTAA CACAACTGTG AAGCAGCTGC TGTCACGCCT GCCAAAGCAA
    AGATACCTCA AACTCGTCTG CGATGAAATT TATAACATCA AAGTTGAAAA GAAGGTGTCA
    GTGCTGTTCC TGTACAGCTA CAGAGATGAC TACTACAGAA TCCTATTTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu30530
    Clone ID Related Accession (Same CDS sequence) XM_017318915.1 , NM_001355383.1
    Accession Version NM_001355383.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4377bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-09
    Organism Mus musculus(house mouse)
    Product eIF-2-alpha kinase GCN2 isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL929163.5. On Sep 15, 2017 this sequence version replaced XM_017318915.1. Transcript Variant: This variant (3) lacks three alternate internal exons in the 5' end compared to variant 1, that causes a frameshift. The resulting isoform (3) has a shorter and distinct N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660819.42515.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383, SAMN00849384 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCCCACCT ACATACCCAG ATGTGAACGT TTGGAAATCA CTAGCTTGAC AAACCAGGAC 
    TATGCCTCTA AGAGAGACCC AGCAGGGCAC AGGGCAGCTG CCATCCTCCA TGGAGGCTCT
    CCTGACTTTG TAGGAAATGG TAAAGCCCGG ACATACTCCT CAGGAAGGTC CAGGCGAGAA
    CGTCAGTATT CTGTGTGTAG TGGTGAACCC TCTCCTGGCT CGTGTGACAT CCTCCATTTC
    TCTGTGGGTA GCCCTGATCA GCTCATGGTG CACAAAGGGA GATGTGTTGG CAGTGATGAG
    CAGCTGGGAA AGGTGGTATA CAATGCTTTG GAAACAGCCA CGGGCAGCTT TGTCTTGCTG
    CATGAGTGGG TCCTTCAATG GCAGAAGATG GGCCCTTGCC TTACCAGTCA AGAGAAAGAG
    AAGATTGACA AGTGCAAAAG GCAGATTCAA GGAGCAGAAA CAGAATTCAG CTCCCTAGTG
    AAACTGAGCC ATCCAAATAT CGTCCGCTAC TTTGCGATGA ACTCCAGAGA GGAGGAGGAC
    TCCATTGTGA TCGACATTCT GGCAGAGCAC GTCAGTGGCA TCTCTCTGGC TACACACCTG
    AGCCACTCGG GCCCAGTCCC CGCCCACCAG CTGCGCAAGT ACACAGCCCA GCTCCTGGCA
    GGCCTGGATT ACCTACACAG CAACTCCGTG GTGCACAAGG TTCTGAGCGC GTCCAGTGTC
    TTGGTAGATG CCGAGGGCAC TGTCAAGATA ACAGACTACA GCATCTCTAA GCGTCTGGCA
    GACATTTGCA AGGAGGATGT ATTTGAGCAA GCTCGAGTTC GTTTTAGTGA CAGTGCCCTG
    CCTTATAAAA CAGGAAAGAA AGGGGATGTG TGGCGGCTCG GCCTCCTGCT GTTGTCTCTC
    AGCCAAGGAC AGGAGTGTGG GGAGTATCCG GTGACCATCC CCAGTGACCT GCCAGCTGAC
    TTCCAAGACT TCCTGAAGAA GTGTGTCTGC CTGGACGACA AGGAAAGATG GAGCCCTCAG
    CAGCTGCTGA AACACAGCTT TATAAACCCT CAACCAAAGC TGCCTTTGGT GGAGCAGAGT
    CCTGAAGACT CTGGGGGACA AGACTACATT GAGACCGTCA TTCCCAGCAA CCAGCTGCCC
    AGCGCTGCGT TCTTCAGTGA GACACAGAAA CAGTTCTCCC GGTACTTCAT TGAGTTTGAA
    GAACTACAAC TCCTAGGGAA AGGAGCTTTT GGAGCTGTCA TCAAGGTGCA AAACAAGCTT
    GATGGCTGCT GCTATGCTGT GAAGCGCATC CCTATCAACC CTGCAAGCAG ACACTTCCGC
    CGGATCAAGG GCGAGGTGAC ACTGCTATCG CGCCTGCACC ATGAGAACAT TGTGCGCTAC
    TACAACGCCT GGATCGAGCG GCATGAGCGC CCAGCGGTGC CAGGGACACC GCCCCCAGAC
    TGCACACCCC AGGCCCAGGA CAGCCCAGCC ACCTGCGGGA AAACATCAGG CGACACTGAA
    GAACTGGGCA GCGTGGAGGC TGCAGCACCG CCACCCATCC TCAGCAGCTC GGTGGAGTGG
    AGCACATCTG CAGAGCGTTC TACCAGCACC CGCTTCCCAG TCACGGGCCA GGATTCCAGC
    AGCGATGAGG AGGACGAGGA CGAGCGCGAT GGCGTCTTCT CCCAGTCCTT TCTACCTGCT
    TCAGATTCTG ACAGTGACAT CATCTTTGAC AATGAAGATG AAAACAGTAA AAGTCAAAAT
    CAGGATGAAG ACTGCAATCA AAAGGACGGT AGCCATGAGA TTGAGCCTTC AGTGACAGCT
    GAGGCTGTGC ACTACCTTTA TATCCAGATG GAGTACTGCG AGAAGAGCAC GCTGCGGGAC
    ACCATTGACC AGGGGCTGTT CCGAGACACC AGCCGGCTCT GGAGGCTTTT CCGAGAGATT
    CTGGATGGAT TAGCTTATAT CCATGAGAAA GGAATGATTC ATCGCGACTT GAAGCCTGTC
    AATATTTTTT TGGATTCTGA TGACCATGTG AAAATAGGTG ACTTTGGCCT GGCAACAGAC
    CATCTGGCCT TCACTGCTGA AGGTAAACAG GACGACCAGG CAGGTGACGG AGTGATTAAG
    TCAGACCCTT CAGGCCATTT GACTGGCATG GTTGGTACTG CTCTGTATGT AAGCCCTGAG
    GTCCAAGGAA GCACCAAGTC TGCATACAAC CAGAAAGTGG ATCTCTTCAG CCTGGGAATT
    ATCTTCTTTG AGATGTCTTA TCACCCGATG GTGACTGCCT CAGAAAGAAT TTTCGTTCTC
    AACCAACTTC GAGATCCCAC ATCGCCCAAG TTTCCAGACG ACTTCGATGA TGGAGAACAT
    ACAAAGCAGA AATCTGTCAT CTCCTGGCTG TTGAACCATG ATCCAGCTAA ACGGCCCACG
    GCCATGGAAC TGCTCAAGAG TGAGCTGCTC CCCCCGCCGC AGATGGAGGA GTCCGAGCTG
    CATGAAGTGC TGCACCACAC GCTGGCCAAC ATAGATGGGA AGGCCTACCG CACCATGATG
    AGTCAGATCT TCTGCCAGCA CATCTCCCCC GCTATAGACT ACACCTACGA CAGTGACATC
    CTGAAGGGCA ACTTCTTGAT TCGTACAGCC AAGATCCAGC AGCTTGTATG TGAAACCATC
    GTCCGCGTCT TTAAAAGGCA TGGCGCTGTC CAGCTGTGCA CCCCACTGCT GCTTCCCCGA
    AACAGGCAAA TATATGAGCA CAACGAAGCG GCTTTGTTCA TGGATCACAG CGGCATGCTG
    GTGATGCTTC CGTTCGACCT GCGGGTCCCT TTTGCAAGAT ATGTGGCAAG AAATAACATA
    CTGAATTTAA AACGATACTG CATAGAGCGG GTGTTCAGAC CTCGGAAGTT AGACCGATTT
    CATCCCAAAG AACTCCTGGA ATGTGCATTT GATATCGTCA CTTCTACCAC CAACAGCTCT
    CTGCCCACTG CCGAAACCAT CTACACCATC TATGAAATAA TCCAGGAGTT TCCTGCACTT
    CAGGAAAGGA ATTACAGCAT TTACTTGAAC CATACGATGC TTCTGAAGGC AATACTCCTA
    CACTGTGGGA TCCCAGAGGA CAAGCTCAGC CAAGTCTACG TCATTCTGTA TGATGCTGTG
    ACAGAGAAGC TGACTAGGAG AGAAGTGGAA GCTAAATTCT GTAACCTGTC CTTGTCTTCT
    AATAGCCTGT GTAGACTCTA CAAGTTCATT GAGCAGAAGG GGGATTTGCA AGACTTAACG
    CCAACCATCA ACTCACTAAT AAAACAGAAA ACAGGCGTTG CTCAGTTGGT GAAGTATAGC
    TTAAAAGACC TCGAGGACGT CGTCGGGCTG CTGAAGAAAC TTGGCGTGAA GTTACAGGTC
    TCCATCAACC TGGGCTTGGT CTACAAGGTG CAGCAGCACA CTGGCATCAT CTTCCAGTTC
    CTGGCATTCA GCAAACGCAG GCAAAGGGTT GTGCCTGAGA TCCTTGCCGC CGGTGGCAGA
    TACGACCTGC TGATTCCCAA GTTCAGAGGC CCACAGACTG TGGGGCCAGT CCCCACTGCT
    GTCGGTGTCA GCATAGCCAT AGACAAGATA TTTGCTGCTG TCCTCAACAT GGAAGAGCCT
    GTTACAGTGA GCTCCTGTGA CCTCCTGGTT GTCAGTGTTG GCCAGATGTC CATGTCCAGG
    GCCATCAACC TAACCCAGAA ACTGTGGACA GCGGGCATCA CTGCAGAAAT CATGTATGAC
    TGGTCCCAGT CCCAGGAAGA GTTACAAGAG TACTGCAGAC ACCATGAAAT CACCTATGTG
    GCTCTGGTCT CCGATAAAGA AGGAAGCCAT GTCAAGGTCA AGTCCTTTGA GAAGGAGAGG
    CAAACAGAAA AGCGTGTATT GGAATCGGAT CTTGTGGATC ACGTTATGCA GAAACTAAGA
    ACCAAAGTTG GTGACGAAAG AAATTTCAGA GACGCTTCTG ATAATCTTGC CGTACAAACT
    CTGAAGGGGT CGTTTTCTAA TGCTTCAGGT TTGTTTGAAA TCCATGGAAC TACAGTAGTC
    CCCAACGTGA TTGTTCTAGC ACCAGAAAAG CTGTCAGCCA GCACCAGGAG GCGACATGAG
    ATTCAGGTGC AAACCCGACT TCAGACTACA CTTGCCAACT TACATCAGAA AAGCAGTGAA
    ATTGAAATTT TGGCTGTGGA CCTACCCAAG GAAACAATCT TACAGTTTCT ATCATTAGAG
    TGGGATGCTG ATGAACAGGC ATTTAACACA ACTGTGAAGC AGCTGCTGTC ACGCCTGCCA
    AAGCAAAGAT ACCTCAAACT CGTCTGCGAT GAAATTTATA ACATCAAAGT TGAAAAGAAG
    GTGTCAGTGC TGTTCCTGTA CAGCTACAGA GATGACTACT ACAGAATCCT ATTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001342312.1
    CDS326..4702
    Translation

    Target ORF information:

    RefSeq Version NM_001355383.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001355383.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCCCACCT ACATACCCAG ATGTGAACGT TTGGAAATCA CTAGCTTGAC AAACCAGGAC 
    TATGCCTCTA AGAGAGACCC AGCAGGGCAC AGGGCAGCTG CCATCCTCCA TGGAGGCTCT
    CCTGACTTTG TAGGAAATGG TAAAGCCCGG ACATACTCCT CAGGAAGGTC CAGGCGAGAA
    CGTCAGTATT CTGTGTGTAG TGGTGAACCC TCTCCTGGCT CGTGTGACAT CCTCCATTTC
    TCTGTGGGTA GCCCTGATCA GCTCATGGTG CACAAAGGGA GATGTGTTGG CAGTGATGAG
    CAGCTGGGAA AGGTGGTATA CAATGCTTTG GAAACAGCCA CGGGCAGCTT TGTCTTGCTG
    CATGAGTGGG TCCTTCAATG GCAGAAGATG GGCCCTTGCC TTACCAGTCA AGAGAAAGAG
    AAGATTGACA AGTGCAAAAG GCAGATTCAA GGAGCAGAAA CAGAATTCAG CTCCCTAGTG
    AAACTGAGCC ATCCAAATAT CGTCCGCTAC TTTGCGATGA ACTCCAGAGA GGAGGAGGAC
    TCCATTGTGA TCGACATTCT GGCAGAGCAC GTCAGTGGCA TCTCTCTGGC TACACACCTG
    AGCCACTCGG GCCCAGTCCC CGCCCACCAG CTGCGCAAGT ACACAGCCCA GCTCCTGGCA
    GGCCTGGATT ACCTACACAG CAACTCCGTG GTGCACAAGG TTCTGAGCGC GTCCAGTGTC
    TTGGTAGATG CCGAGGGCAC TGTCAAGATA ACAGACTACA GCATCTCTAA GCGTCTGGCA
    GACATTTGCA AGGAGGATGT ATTTGAGCAA GCTCGAGTTC GTTTTAGTGA CAGTGCCCTG
    CCTTATAAAA CAGGAAAGAA AGGGGATGTG TGGCGGCTCG GCCTCCTGCT GTTGTCTCTC
    AGCCAAGGAC AGGAGTGTGG GGAGTATCCG GTGACCATCC CCAGTGACCT GCCAGCTGAC
    TTCCAAGACT TCCTGAAGAA GTGTGTCTGC CTGGACGACA AGGAAAGATG GAGCCCTCAG
    CAGCTGCTGA AACACAGCTT TATAAACCCT CAACCAAAGC TGCCTTTGGT GGAGCAGAGT
    CCTGAAGACT CTGGGGGACA AGACTACATT GAGACCGTCA TTCCCAGCAA CCAGCTGCCC
    AGCGCTGCGT TCTTCAGTGA GACACAGAAA CAGTTCTCCC GGTACTTCAT TGAGTTTGAA
    GAACTACAAC TCCTAGGGAA AGGAGCTTTT GGAGCTGTCA TCAAGGTGCA AAACAAGCTT
    GATGGCTGCT GCTATGCTGT GAAGCGCATC CCTATCAACC CTGCAAGCAG ACACTTCCGC
    CGGATCAAGG GCGAGGTGAC ACTGCTATCG CGCCTGCACC ATGAGAACAT TGTGCGCTAC
    TACAACGCCT GGATCGAGCG GCATGAGCGC CCAGCGGTGC CAGGGACACC GCCCCCAGAC
    TGCACACCCC AGGCCCAGGA CAGCCCAGCC ACCTGCGGGA AAACATCAGG CGACACTGAA
    GAACTGGGCA GCGTGGAGGC TGCAGCACCG CCACCCATCC TCAGCAGCTC GGTGGAGTGG
    AGCACATCTG CAGAGCGTTC TACCAGCACC CGCTTCCCAG TCACGGGCCA GGATTCCAGC
    AGCGATGAGG AGGACGAGGA CGAGCGCGAT GGCGTCTTCT CCCAGTCCTT TCTACCTGCT
    TCAGATTCTG ACAGTGACAT CATCTTTGAC AATGAAGATG AAAACAGTAA AAGTCAAAAT
    CAGGATGAAG ACTGCAATCA AAAGGACGGT AGCCATGAGA TTGAGCCTTC AGTGACAGCT
    GAGGCTGTGC ACTACCTTTA TATCCAGATG GAGTACTGCG AGAAGAGCAC GCTGCGGGAC
    ACCATTGACC AGGGGCTGTT CCGAGACACC AGCCGGCTCT GGAGGCTTTT CCGAGAGATT
    CTGGATGGAT TAGCTTATAT CCATGAGAAA GGAATGATTC ATCGCGACTT GAAGCCTGTC
    AATATTTTTT TGGATTCTGA TGACCATGTG AAAATAGGTG ACTTTGGCCT GGCAACAGAC
    CATCTGGCCT TCACTGCTGA AGGTAAACAG GACGACCAGG CAGGTGACGG AGTGATTAAG
    TCAGACCCTT CAGGCCATTT GACTGGCATG GTTGGTACTG CTCTGTATGT AAGCCCTGAG
    GTCCAAGGAA GCACCAAGTC TGCATACAAC CAGAAAGTGG ATCTCTTCAG CCTGGGAATT
    ATCTTCTTTG AGATGTCTTA TCACCCGATG GTGACTGCCT CAGAAAGAAT TTTCGTTCTC
    AACCAACTTC GAGATCCCAC ATCGCCCAAG TTTCCAGACG ACTTCGATGA TGGAGAACAT
    ACAAAGCAGA AATCTGTCAT CTCCTGGCTG TTGAACCATG ATCCAGCTAA ACGGCCCACG
    GCCATGGAAC TGCTCAAGAG TGAGCTGCTC CCCCCGCCGC AGATGGAGGA GTCCGAGCTG
    CATGAAGTGC TGCACCACAC GCTGGCCAAC ATAGATGGGA AGGCCTACCG CACCATGATG
    AGTCAGATCT TCTGCCAGCA CATCTCCCCC GCTATAGACT ACACCTACGA CAGTGACATC
    CTGAAGGGCA ACTTCTTGAT TCGTACAGCC AAGATCCAGC AGCTTGTATG TGAAACCATC
    GTCCGCGTCT TTAAAAGGCA TGGCGCTGTC CAGCTGTGCA CCCCACTGCT GCTTCCCCGA
    AACAGGCAAA TATATGAGCA CAACGAAGCG GCTTTGTTCA TGGATCACAG CGGCATGCTG
    GTGATGCTTC CGTTCGACCT GCGGGTCCCT TTTGCAAGAT ATGTGGCAAG AAATAACATA
    CTGAATTTAA AACGATACTG CATAGAGCGG GTGTTCAGAC CTCGGAAGTT AGACCGATTT
    CATCCCAAAG AACTCCTGGA ATGTGCATTT GATATCGTCA CTTCTACCAC CAACAGCTCT
    CTGCCCACTG CCGAAACCAT CTACACCATC TATGAAATAA TCCAGGAGTT TCCTGCACTT
    CAGGAAAGGA ATTACAGCAT TTACTTGAAC CATACGATGC TTCTGAAGGC AATACTCCTA
    CACTGTGGGA TCCCAGAGGA CAAGCTCAGC CAAGTCTACG TCATTCTGTA TGATGCTGTG
    ACAGAGAAGC TGACTAGGAG AGAAGTGGAA GCTAAATTCT GTAACCTGTC CTTGTCTTCT
    AATAGCCTGT GTAGACTCTA CAAGTTCATT GAGCAGAAGG GGGATTTGCA AGACTTAACG
    CCAACCATCA ACTCACTAAT AAAACAGAAA ACAGGCGTTG CTCAGTTGGT GAAGTATAGC
    TTAAAAGACC TCGAGGACGT CGTCGGGCTG CTGAAGAAAC TTGGCGTGAA GTTACAGGTC
    TCCATCAACC TGGGCTTGGT CTACAAGGTG CAGCAGCACA CTGGCATCAT CTTCCAGTTC
    CTGGCATTCA GCAAACGCAG GCAAAGGGTT GTGCCTGAGA TCCTTGCCGC CGGTGGCAGA
    TACGACCTGC TGATTCCCAA GTTCAGAGGC CCACAGACTG TGGGGCCAGT CCCCACTGCT
    GTCGGTGTCA GCATAGCCAT AGACAAGATA TTTGCTGCTG TCCTCAACAT GGAAGAGCCT
    GTTACAGTGA GCTCCTGTGA CCTCCTGGTT GTCAGTGTTG GCCAGATGTC CATGTCCAGG
    GCCATCAACC TAACCCAGAA ACTGTGGACA GCGGGCATCA CTGCAGAAAT CATGTATGAC
    TGGTCCCAGT CCCAGGAAGA GTTACAAGAG TACTGCAGAC ACCATGAAAT CACCTATGTG
    GCTCTGGTCT CCGATAAAGA AGGAAGCCAT GTCAAGGTCA AGTCCTTTGA GAAGGAGAGG
    CAAACAGAAA AGCGTGTATT GGAATCGGAT CTTGTGGATC ACGTTATGCA GAAACTAAGA
    ACCAAAGTTG GTGACGAAAG AAATTTCAGA GACGCTTCTG ATAATCTTGC CGTACAAACT
    CTGAAGGGGT CGTTTTCTAA TGCTTCAGGT TTGTTTGAAA TCCATGGAAC TACAGTAGTC
    CCCAACGTGA TTGTTCTAGC ACCAGAAAAG CTGTCAGCCA GCACCAGGAG GCGACATGAG
    ATTCAGGTGC AAACCCGACT TCAGACTACA CTTGCCAACT TACATCAGAA AAGCAGTGAA
    ATTGAAATTT TGGCTGTGGA CCTACCCAAG GAAACAATCT TACAGTTTCT ATCATTAGAG
    TGGGATGCTG ATGAACAGGC ATTTAACACA ACTGTGAAGC AGCTGCTGTC ACGCCTGCCA
    AAGCAAAGAT ACCTCAAACT CGTCTGCGAT GAAATTTATA ACATCAAAGT TGAAAAGAAG
    GTGTCAGTGC TGTTCCTGTA CAGCTACAGA GATGACTACT ACAGAATCCT ATTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu17792
    Clone ID Related Accession (Same CDS sequence) NM_001177806.1 , XM_006499625.3 , XM_006499625.4
    Accession Version XM_006499625.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4611bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product eIF-2-alpha kinase GCN2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006499625.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCCCACCT ACATACCCAG ATGTGTGATG ATATTTGAAC TGGCACACCA TGTGCAGTCG 
    TTTCTCAGCG AGCATAACAA GCCCCCACCA AAGTCTTTCC ATGAAGAAAT GCTGGAAAGG
    CAGGCTCAGG AGAAGCAGCA GAGGTTGCTG GAGGCCAGGC GGAAGGAGGA GCAGGAGCAA
    CGTGAAATCC TCCACGAGAT TCAGAGAAGG AAAGAGGAGA TCAAGGAAGA GAAGAAAAGG
    AAGGAAATGG CTAAGCAGGA ACGTTTGGAA ATCACTAGCT TGACAAACCA GGACTATGCC
    TCTAAGAGAG ACCCAGCAGG GCACAGGGCA GCTGCCATCC TCCATGGAGG CTCTCCTGAC
    TTTGTAGGAA ATGGTAAAGC CCGGACATAC TCCTCAGGAA GGTCCAGGCG AGAACGTCAG
    TATTCTGTGT GTAGTGGTGA ACCCTCTCCT GGCTCGTGTG ACATCCTCCA TTTCTCTGTG
    GGTAGCCCTG ATCAGCTCAT GGTGCACAAA GGGAGATGTG TTGGCAGTGA TGAGCAGCTG
    GGAAAGGTGG TATACAATGC TTTGGAAACA GCCACGGGCA GCTTTGTCTT GCTGCATGAG
    TGGGTCCTTC AATGGCAGAA GATGGGCCCT TGCCTTACCA GTCAAGAGAA AGAGAAGATT
    GACAAGTGCA AAAGGCAGAT TCAAGGAGCA GAAACAGAAT TCAGCTCCCT AGTGAAACTG
    AGCCATCCAA ATATCGTCCG CTACTTTGCG ATGAACTCCA GAGAGGAGGA GGACTCCATT
    GTGATCGACA TTCTGGCAGA GCACGTCAGT GGCATCTCTC TGGCTACACA CCTGAGCCAC
    TCGGGCCCAG TCCCCGCCCA CCAGCTGCGC AAGTACACAG CCCAGCTCCT GGCAGGCCTG
    GATTACCTAC ACAGCAACTC CGTGGTGCAC AAGGTTCTGA GCGCGTCCAG TGTCTTGGTA
    GATGCCGAGG GCACTGTCAA GATAACAGAC TACAGCATCT CTAAGCGTCT GGCAGACATT
    TGCAAGGAGG ATGTATTTGA GCAAGCTCGA GTTCGTTTTA GTGACAGTGC CCTGCCTTAT
    AAAACAGGAA AGAAAGGGGA TGTGTGGCGG CTCGGCCTCC TGCTGTTGTC TCTCAGCCAA
    GGACAGGAGT GTGGGGAGTA TCCGGTGACC ATCCCCAGTG ACCTGCCAGC TGACTTCCAA
    GACTTCCTGA AGAAGTGTGT CTGCCTGGAC GACAAGGAAA GATGGAGCCC TCAGCAGCTG
    CTGAAACACA GCTTTATAAA CCCTCAACCA AAGCTGCCTT TGGTGGAGCA GAGTCCTGAA
    GACTCTGGGG GACAAGACTA CATTGAGACC GTCATTCCCA GCAACCAGCT GCCCAGCGCT
    GCGTTCTTCA GTGAGACACA GAAACAGTTC TCCCGGTACT TCATTGAGTT TGAAGAACTA
    CAACTCCTAG GGAAAGGAGC TTTTGGAGCT GTCATCAAGG TGCAAAACAA GCTTGATGGC
    TGCTGCTATG CTGTGAAGCG CATCCCTATC AACCCTGCAA GCAGACACTT CCGCCGGATC
    AAGGGCGAGG TGACACTGCT ATCGCGCCTG CACCATGAGA ACATTGTGCG CTACTACAAC
    GCCTGGATCG AGCGGCATGA GCGCCCAGCG GTGCCAGGGA CACCGCCCCC AGACTGCACA
    CCCCAGGCCC AGGACAGCCC AGCCACCTGC GGGAAAACAT CAGGCGACAC TGAAGAACTG
    GGCAGCGTGG AGGCTGCAGC ACCGCCACCC ATCCTCAGCA GCTCGGTGGA GTGGAGCACA
    TCTGCAGAGC GTTCTACCAG CACCCGCTTC CCAGTCACGG GCCAGGATTC CAGCAGCGAT
    GAGGAGGACG AGGACGAGCG CGATGGCGTC TTCTCCCAGT CCTTTCTACC TGCTTCAGAT
    TCTGACAGTG ACATCATCTT TGACAATGAA GATGAAAACA GTAAAAGTCA AAATCAGGAT
    GAAGACTGCA ATCAAAAGGA CGGTAGCCAT GAGATTGAGC CTTCAGTGAC AGCTGAGGCT
    GTGCACTACC TTTATATCCA GATGGAGTAC TGCGAGAAGA GCACGCTGCG GGACACCATT
    GACCAGGGGC TGTTCCGAGA CACCAGCCGG CTCTGGAGGC TTTTCCGAGA GATTCTGGAT
    GGATTAGCTT ATATCCATGA GAAAGGAATG ATTCATCGCG ACTTGAAGCC TGTCAATATT
    TTTTTGGATT CTGATGACCA TGTGAAAATA GGTGACTTTG GCCTGGCAAC AGACCATCTG
    GCCTTCACTG CTGAAGGTAA ACAGGACGAC CAGGCAGGTG ACGGAGTGAT TAAGTCAGAC
    CCTTCAGGCC ATTTGACTGG CATGGTTGGT ACTGCTCTGT ATGTAAGCCC TGAGGTCCAA
    GGAAGCACCA AGTCTGCATA CAACCAGAAA GTGGATCTCT TCAGCCTGGG AATTATCTTC
    TTTGAGATGT CTTATCACCC GATGGTGACT GCCTCAGAAA GAATTTTCGT TCTCAACCAA
    CTTCGAGATC CCACATCGCC CAAGTTTCCA GACGACTTCG ATGATGGAGA ACATACAAAG
    CAGAAATCTG TCATCTCCTG GCTGTTGAAC CATGATCCAG CTAAACGGCC CACGGCCATG
    GAACTGCTCA AGAGTGAGCT GCTCCCCCCG CCGCAGATGG AGGAGTCCGA GCTGCATGAA
    GTGCTGCACC ACACGCTGGC CAACATAGAT GGGAAGGCCT ACCGCACCAT GATGAGTCAG
    ATCTTCTGCC AGCACATCTC CCCCGCTATA GACTACACCT ACGACAGTGA CATCCTGAAG
    GGCAACTTCT TGATTCGTAC AGCCAAGATC CAGCAGCTTG TATGTGAAAC CATCGTCCGC
    GTCTTTAAAA GGCATGGCGC TGTCCAGCTG TGCACCCCAC TGCTGCTTCC CCGAAACAGG
    CAAATATATG AGCACAACGA AGCGGCTTTG TTCATGGATC ACAGCGGCAT GCTGGTGATG
    CTTCCGTTCG ACCTGCGGGT CCCTTTTGCA AGATATGTGG CAAGAAATAA CATACTGAAT
    TTAAAACGAT ACTGCATAGA GCGGGTGTTC AGACCTCGGA AGTTAGACCG ATTTCATCCC
    AAAGAACTCC TGGAATGTGC ATTTGATATC GTCACTTCTA CCACCAACAG CTCTCTGCCC
    ACTGCCGAAA CCATCTACAC CATCTATGAA ATAATCCAGG AGTTTCCTGC ACTTCAGGAA
    AGGAATTACA GCATTTACTT GAACCATACG ATGCTTCTGA AGGCAATACT CCTACACTGT
    GGGATCCCAG AGGACAAGCT CAGCCAAGTC TACGTCATTC TGTATGATGC TGTGACAGAG
    AAGCTGACTA GGAGAGAAGT GGAAGCTAAA TTCTGTAACC TGTCCTTGTC TTCTAATAGC
    CTGTGTAGAC TCTACAAGTT CATTGAGCAG AAGGGGGATT TGCAAGACTT AACGCCAACC
    ATCAACTCAC TAATAAAACA GAAAACAGGC GTTGCTCAGT TGGTGAAGTA TAGCTTAAAA
    GACCTCGAGG ACGTCGTCGG GCTGCTGAAG AAACTTGGCG TGAAGTTACA GGTCTCCATC
    AACCTGGGCT TGGTCTACAA GGTGCAGCAG CACACTGGCA TCATCTTCCA GTTCCTGGCA
    TTCAGCAAAC GCAGGCAAAG GGTTGTGCCT GAGATCCTTG CCGCCGGTGG CAGATACGAC
    CTGCTGATTC CCAAGTTCAG AGGCCCACAG ACTGTGGGGC CAGTCCCCAC TGCTGTCGGT
    GTCAGCATAG CCATAGACAA GATATTTGCT GCTGTCCTCA ACATGGAAGA GCCTGTTACA
    GTGAGCTCCT GTGACCTCCT GGTTGTCAGT GTTGGCCAGA TGTCCATGTC CAGGGCCATC
    AACCTAACCC AGAAACTGTG GACAGCGGGC ATCACTGCAG AAATCATGTA TGACTGGTCC
    CAGTCCCAGG AAGAGTTACA AGAGTACTGC AGACACCATG AAATCACCTA TGTGGCTCTG
    GTCTCCGATA AAGAAGGAAG CCATGTCAAG GTCAAGTCCT TTGAGAAGGA GAGGCAAACA
    GAAAAGCGTG TATTGGAATC GGATCTTGTG GATCACGTTA TGCAGAAACT AAGAACCAAA
    GTTGGTGACG AAAGAAATTT CAGAGACGCT TCTGATAATC TTGCCGTACA AACTCTGAAG
    GGGTCGTTTT CTAATGCTTC AGGTTTGTTT GAAATCCATG GAACTACAGT AGTCCCCAAC
    GTGATTGTTC TAGCACCAGA AAAGCTGTCA GCCAGCACCA GGAGGCGACA TGAGATTCAG
    GTGCAAACCC GACTTCAGAC TACACTTGCC AACTTACATC AGAAAAGCAG TGAAATTGAA
    ATTTTGGCTG TGGACCTACC CAAGGAAACA ATCTTACAGT TTCTATCATT AGAGTGGGAT
    GCTGATGAAC AGGCATTTAA CACAACTGTG AAGCAGCTGC TGTCACGCCT GCCAAAGCAA
    AGATACCTCA AACTCGTCTG CGATGAAATT TATAACATCA AAGTTGAAAA GAAGGTGTCA
    GTGCTGTTCC TGTACAGCTA CAGAGATGAC TACTACAGAA TCCTATTTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006499688.1
    CDS361..4971
    Translation

    Target ORF information:

    RefSeq Version XM_006499625.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006499625.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCCCACCT ACATACCCAG ATGTGTGATG ATATTTGAAC TGGCACACCA TGTGCAGTCG 
    TTTCTCAGCG AGCATAACAA GCCCCCACCA AAGTCTTTCC ATGAAGAAAT GCTGGAAAGG
    CAGGCTCAGG AGAAGCAGCA GAGGTTGCTG GAGGCCAGGC GGAAGGAGGA GCAGGAGCAA
    CGTGAAATCC TCCACGAGAT TCAGAGAAGG AAAGAGGAGA TCAAGGAAGA GAAGAAAAGG
    AAGGAAATGG CTAAGCAGGA ACGTTTGGAA ATCACTAGCT TGACAAACCA GGACTATGCC
    TCTAAGAGAG ACCCAGCAGG GCACAGGGCA GCTGCCATCC TCCATGGAGG CTCTCCTGAC
    TTTGTAGGAA ATGGTAAAGC CCGGACATAC TCCTCAGGAA GGTCCAGGCG AGAACGTCAG
    TATTCTGTGT GTAGTGGTGA ACCCTCTCCT GGCTCGTGTG ACATCCTCCA TTTCTCTGTG
    GGTAGCCCTG ATCAGCTCAT GGTGCACAAA GGGAGATGTG TTGGCAGTGA TGAGCAGCTG
    GGAAAGGTGG TATACAATGC TTTGGAAACA GCCACGGGCA GCTTTGTCTT GCTGCATGAG
    TGGGTCCTTC AATGGCAGAA GATGGGCCCT TGCCTTACCA GTCAAGAGAA AGAGAAGATT
    GACAAGTGCA AAAGGCAGAT TCAAGGAGCA GAAACAGAAT TCAGCTCCCT AGTGAAACTG
    AGCCATCCAA ATATCGTCCG CTACTTTGCG ATGAACTCCA GAGAGGAGGA GGACTCCATT
    GTGATCGACA TTCTGGCAGA GCACGTCAGT GGCATCTCTC TGGCTACACA CCTGAGCCAC
    TCGGGCCCAG TCCCCGCCCA CCAGCTGCGC AAGTACACAG CCCAGCTCCT GGCAGGCCTG
    GATTACCTAC ACAGCAACTC CGTGGTGCAC AAGGTTCTGA GCGCGTCCAG TGTCTTGGTA
    GATGCCGAGG GCACTGTCAA GATAACAGAC TACAGCATCT CTAAGCGTCT GGCAGACATT
    TGCAAGGAGG ATGTATTTGA GCAAGCTCGA GTTCGTTTTA GTGACAGTGC CCTGCCTTAT
    AAAACAGGAA AGAAAGGGGA TGTGTGGCGG CTCGGCCTCC TGCTGTTGTC TCTCAGCCAA
    GGACAGGAGT GTGGGGAGTA TCCGGTGACC ATCCCCAGTG ACCTGCCAGC TGACTTCCAA
    GACTTCCTGA AGAAGTGTGT CTGCCTGGAC GACAAGGAAA GATGGAGCCC TCAGCAGCTG
    CTGAAACACA GCTTTATAAA CCCTCAACCA AAGCTGCCTT TGGTGGAGCA GAGTCCTGAA
    GACTCTGGGG GACAAGACTA CATTGAGACC GTCATTCCCA GCAACCAGCT GCCCAGCGCT
    GCGTTCTTCA GTGAGACACA GAAACAGTTC TCCCGGTACT TCATTGAGTT TGAAGAACTA
    CAACTCCTAG GGAAAGGAGC TTTTGGAGCT GTCATCAAGG TGCAAAACAA GCTTGATGGC
    TGCTGCTATG CTGTGAAGCG CATCCCTATC AACCCTGCAA GCAGACACTT CCGCCGGATC
    AAGGGCGAGG TGACACTGCT ATCGCGCCTG CACCATGAGA ACATTGTGCG CTACTACAAC
    GCCTGGATCG AGCGGCATGA GCGCCCAGCG GTGCCAGGGA CACCGCCCCC AGACTGCACA
    CCCCAGGCCC AGGACAGCCC AGCCACCTGC GGGAAAACAT CAGGCGACAC TGAAGAACTG
    GGCAGCGTGG AGGCTGCAGC ACCGCCACCC ATCCTCAGCA GCTCGGTGGA GTGGAGCACA
    TCTGCAGAGC GTTCTACCAG CACCCGCTTC CCAGTCACGG GCCAGGATTC CAGCAGCGAT
    GAGGAGGACG AGGACGAGCG CGATGGCGTC TTCTCCCAGT CCTTTCTACC TGCTTCAGAT
    TCTGACAGTG ACATCATCTT TGACAATGAA GATGAAAACA GTAAAAGTCA AAATCAGGAT
    GAAGACTGCA ATCAAAAGGA CGGTAGCCAT GAGATTGAGC CTTCAGTGAC AGCTGAGGCT
    GTGCACTACC TTTATATCCA GATGGAGTAC TGCGAGAAGA GCACGCTGCG GGACACCATT
    GACCAGGGGC TGTTCCGAGA CACCAGCCGG CTCTGGAGGC TTTTCCGAGA GATTCTGGAT
    GGATTAGCTT ATATCCATGA GAAAGGAATG ATTCATCGCG ACTTGAAGCC TGTCAATATT
    TTTTTGGATT CTGATGACCA TGTGAAAATA GGTGACTTTG GCCTGGCAAC AGACCATCTG
    GCCTTCACTG CTGAAGGTAA ACAGGACGAC CAGGCAGGTG ACGGAGTGAT TAAGTCAGAC
    CCTTCAGGCC ATTTGACTGG CATGGTTGGT ACTGCTCTGT ATGTAAGCCC TGAGGTCCAA
    GGAAGCACCA AGTCTGCATA CAACCAGAAA GTGGATCTCT TCAGCCTGGG AATTATCTTC
    TTTGAGATGT CTTATCACCC GATGGTGACT GCCTCAGAAA GAATTTTCGT TCTCAACCAA
    CTTCGAGATC CCACATCGCC CAAGTTTCCA GACGACTTCG ATGATGGAGA ACATACAAAG
    CAGAAATCTG TCATCTCCTG GCTGTTGAAC CATGATCCAG CTAAACGGCC CACGGCCATG
    GAACTGCTCA AGAGTGAGCT GCTCCCCCCG CCGCAGATGG AGGAGTCCGA GCTGCATGAA
    GTGCTGCACC ACACGCTGGC CAACATAGAT GGGAAGGCCT ACCGCACCAT GATGAGTCAG
    ATCTTCTGCC AGCACATCTC CCCCGCTATA GACTACACCT ACGACAGTGA CATCCTGAAG
    GGCAACTTCT TGATTCGTAC AGCCAAGATC CAGCAGCTTG TATGTGAAAC CATCGTCCGC
    GTCTTTAAAA GGCATGGCGC TGTCCAGCTG TGCACCCCAC TGCTGCTTCC CCGAAACAGG
    CAAATATATG AGCACAACGA AGCGGCTTTG TTCATGGATC ACAGCGGCAT GCTGGTGATG
    CTTCCGTTCG ACCTGCGGGT CCCTTTTGCA AGATATGTGG CAAGAAATAA CATACTGAAT
    TTAAAACGAT ACTGCATAGA GCGGGTGTTC AGACCTCGGA AGTTAGACCG ATTTCATCCC
    AAAGAACTCC TGGAATGTGC ATTTGATATC GTCACTTCTA CCACCAACAG CTCTCTGCCC
    ACTGCCGAAA CCATCTACAC CATCTATGAA ATAATCCAGG AGTTTCCTGC ACTTCAGGAA
    AGGAATTACA GCATTTACTT GAACCATACG ATGCTTCTGA AGGCAATACT CCTACACTGT
    GGGATCCCAG AGGACAAGCT CAGCCAAGTC TACGTCATTC TGTATGATGC TGTGACAGAG
    AAGCTGACTA GGAGAGAAGT GGAAGCTAAA TTCTGTAACC TGTCCTTGTC TTCTAATAGC
    CTGTGTAGAC TCTACAAGTT CATTGAGCAG AAGGGGGATT TGCAAGACTT AACGCCAACC
    ATCAACTCAC TAATAAAACA GAAAACAGGC GTTGCTCAGT TGGTGAAGTA TAGCTTAAAA
    GACCTCGAGG ACGTCGTCGG GCTGCTGAAG AAACTTGGCG TGAAGTTACA GGTCTCCATC
    AACCTGGGCT TGGTCTACAA GGTGCAGCAG CACACTGGCA TCATCTTCCA GTTCCTGGCA
    TTCAGCAAAC GCAGGCAAAG GGTTGTGCCT GAGATCCTTG CCGCCGGTGG CAGATACGAC
    CTGCTGATTC CCAAGTTCAG AGGCCCACAG ACTGTGGGGC CAGTCCCCAC TGCTGTCGGT
    GTCAGCATAG CCATAGACAA GATATTTGCT GCTGTCCTCA ACATGGAAGA GCCTGTTACA
    GTGAGCTCCT GTGACCTCCT GGTTGTCAGT GTTGGCCAGA TGTCCATGTC CAGGGCCATC
    AACCTAACCC AGAAACTGTG GACAGCGGGC ATCACTGCAG AAATCATGTA TGACTGGTCC
    CAGTCCCAGG AAGAGTTACA AGAGTACTGC AGACACCATG AAATCACCTA TGTGGCTCTG
    GTCTCCGATA AAGAAGGAAG CCATGTCAAG GTCAAGTCCT TTGAGAAGGA GAGGCAAACA
    GAAAAGCGTG TATTGGAATC GGATCTTGTG GATCACGTTA TGCAGAAACT AAGAACCAAA
    GTTGGTGACG AAAGAAATTT CAGAGACGCT TCTGATAATC TTGCCGTACA AACTCTGAAG
    GGGTCGTTTT CTAATGCTTC AGGTTTGTTT GAAATCCATG GAACTACAGT AGTCCCCAAC
    GTGATTGTTC TAGCACCAGA AAAGCTGTCA GCCAGCACCA GGAGGCGACA TGAGATTCAG
    GTGCAAACCC GACTTCAGAC TACACTTGCC AACTTACATC AGAAAAGCAG TGAAATTGAA
    ATTTTGGCTG TGGACCTACC CAAGGAAACA ATCTTACAGT TTCTATCATT AGAGTGGGAT
    GCTGATGAAC AGGCATTTAA CACAACTGTG AAGCAGCTGC TGTCACGCCT GCCAAAGCAA
    AGATACCTCA AACTCGTCTG CGATGAAATT TATAACATCA AAGTTGAAAA GAAGGTGTCA
    GTGCTGTTCC TGTACAGCTA CAGAGATGAC TACTACAGAA TCCTATTTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu30528
    Clone ID Related Accession (Same CDS sequence) XM_006499626.3 , XM_006499626.4
    Accession Version XM_006499626.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4578bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product eIF-2-alpha kinase GCN2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006499626.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGCTGGGG GCCGCGGGGC CTCTGGACGC GGCCGGGCTG AGCCACAGGA GAGCTATTCG 
    CAGCGACAGG ACCACGAGCT GCAGGCCCTG GAGGCCATTT ACGGCTCGGA CTTCCAGGAT
    CTGCGGCCGG ATGCGCGCGG ACGGCAACGT GAAATCCTCC ACGAGATTCA GAGAAGGAAA
    GAGGAGATCA AGGAAGAGAA GAAAAGGAAG GAAATGGCTA AGCAGGAACG TTTGGAAATC
    ACTAGCTTGA CAAACCAGGA CTATGCCTCT AAGAGAGACC CAGCAGGGCA CAGGGCAGCT
    GCCATCCTCC ATGGAGGCTC TCCTGACTTT GTAGGAAATG GTAAAGCCCG GACATACTCC
    TCAGGAAGGT CCAGGCGAGA ACGTCAGTAT TCTGTGTGTA GTGGTGAACC CTCTCCTGGC
    TCGTGTGACA TCCTCCATTT CTCTGTGGGT AGCCCTGATC AGCTCATGGT GCACAAAGGG
    AGATGTGTTG GCAGTGATGA GCAGCTGGGA AAGGTGGTAT ACAATGCTTT GGAAACAGCC
    ACGGGCAGCT TTGTCTTGCT GCATGAGTGG GTCCTTCAAT GGCAGAAGAT GGGCCCTTGC
    CTTACCAGTC AAGAGAAAGA GAAGATTGAC AAGTGCAAAA GGCAGATTCA AGGAGCAGAA
    ACAGAATTCA GCTCCCTAGT GAAACTGAGC CATCCAAATA TCGTCCGCTA CTTTGCGATG
    AACTCCAGAG AGGAGGAGGA CTCCATTGTG ATCGACATTC TGGCAGAGCA CGTCAGTGGC
    ATCTCTCTGG CTACACACCT GAGCCACTCG GGCCCAGTCC CCGCCCACCA GCTGCGCAAG
    TACACAGCCC AGCTCCTGGC AGGCCTGGAT TACCTACACA GCAACTCCGT GGTGCACAAG
    GTTCTGAGCG CGTCCAGTGT CTTGGTAGAT GCCGAGGGCA CTGTCAAGAT AACAGACTAC
    AGCATCTCTA AGCGTCTGGC AGACATTTGC AAGGAGGATG TATTTGAGCA AGCTCGAGTT
    CGTTTTAGTG ACAGTGCCCT GCCTTATAAA ACAGGAAAGA AAGGGGATGT GTGGCGGCTC
    GGCCTCCTGC TGTTGTCTCT CAGCCAAGGA CAGGAGTGTG GGGAGTATCC GGTGACCATC
    CCCAGTGACC TGCCAGCTGA CTTCCAAGAC TTCCTGAAGA AGTGTGTCTG CCTGGACGAC
    AAGGAAAGAT GGAGCCCTCA GCAGCTGCTG AAACACAGCT TTATAAACCC TCAACCAAAG
    CTGCCTTTGG TGGAGCAGAG TCCTGAAGAC TCTGGGGGAC AAGACTACAT TGAGACCGTC
    ATTCCCAGCA ACCAGCTGCC CAGCGCTGCG TTCTTCAGTG AGACACAGAA ACAGTTCTCC
    CGGTACTTCA TTGAGTTTGA AGAACTACAA CTCCTAGGGA AAGGAGCTTT TGGAGCTGTC
    ATCAAGGTGC AAAACAAGCT TGATGGCTGC TGCTATGCTG TGAAGCGCAT CCCTATCAAC
    CCTGCAAGCA GACACTTCCG CCGGATCAAG GGCGAGGTGA CACTGCTATC GCGCCTGCAC
    CATGAGAACA TTGTGCGCTA CTACAACGCC TGGATCGAGC GGCATGAGCG CCCAGCGGTG
    CCAGGGACAC CGCCCCCAGA CTGCACACCC CAGGCCCAGG ACAGCCCAGC CACCTGCGGG
    AAAACATCAG GCGACACTGA AGAACTGGGC AGCGTGGAGG CTGCAGCACC GCCACCCATC
    CTCAGCAGCT CGGTGGAGTG GAGCACATCT GCAGAGCGTT CTACCAGCAC CCGCTTCCCA
    GTCACGGGCC AGGATTCCAG CAGCGATGAG GAGGACGAGG ACGAGCGCGA TGGCGTCTTC
    TCCCAGTCCT TTCTACCTGC TTCAGATTCT GACAGTGACA TCATCTTTGA CAATGAAGAT
    GAAAACAGTA AAAGTCAAAA TCAGGATGAA GACTGCAATC AAAAGGACGG TAGCCATGAG
    ATTGAGCCTT CAGTGACAGC TGAGGCTGTG CACTACCTTT ATATCCAGAT GGAGTACTGC
    GAGAAGAGCA CGCTGCGGGA CACCATTGAC CAGGGGCTGT TCCGAGACAC CAGCCGGCTC
    TGGAGGCTTT TCCGAGAGAT TCTGGATGGA TTAGCTTATA TCCATGAGAA AGGAATGATT
    CATCGCGACT TGAAGCCTGT CAATATTTTT TTGGATTCTG ATGACCATGT GAAAATAGGT
    GACTTTGGCC TGGCAACAGA CCATCTGGCC TTCACTGCTG AAGGTAAACA GGACGACCAG
    GCAGGTGACG GAGTGATTAA GTCAGACCCT TCAGGCCATT TGACTGGCAT GGTTGGTACT
    GCTCTGTATG TAAGCCCTGA GGTCCAAGGA AGCACCAAGT CTGCATACAA CCAGAAAGTG
    GATCTCTTCA GCCTGGGAAT TATCTTCTTT GAGATGTCTT ATCACCCGAT GGTGACTGCC
    TCAGAAAGAA TTTTCGTTCT CAACCAACTT CGAGATCCCA CATCGCCCAA GTTTCCAGAC
    GACTTCGATG ATGGAGAACA TACAAAGCAG AAATCTGTCA TCTCCTGGCT GTTGAACCAT
    GATCCAGCTA AACGGCCCAC GGCCATGGAA CTGCTCAAGA GTGAGCTGCT CCCCCCGCCG
    CAGATGGAGG AGTCCGAGCT GCATGAAGTG CTGCACCACA CGCTGGCCAA CATAGATGGG
    AAGGCCTACC GCACCATGAT GAGTCAGATC TTCTGCCAGC ACATCTCCCC CGCTATAGAC
    TACACCTACG ACAGTGACAT CCTGAAGGGC AACTTCTTGA TTCGTACAGC CAAGATCCAG
    CAGCTTGTAT GTGAAACCAT CGTCCGCGTC TTTAAAAGGC ATGGCGCTGT CCAGCTGTGC
    ACCCCACTGC TGCTTCCCCG AAACAGGCAA ATATATGAGC ACAACGAAGC GGCTTTGTTC
    ATGGATCACA GCGGCATGCT GGTGATGCTT CCGTTCGACC TGCGGGTCCC TTTTGCAAGA
    TATGTGGCAA GAAATAACAT ACTGAATTTA AAACGATACT GCATAGAGCG GGTGTTCAGA
    CCTCGGAAGT TAGACCGATT TCATCCCAAA GAACTCCTGG AATGTGCATT TGATATCGTC
    ACTTCTACCA CCAACAGCTC TCTGCCCACT GCCGAAACCA TCTACACCAT CTATGAAATA
    ATCCAGGAGT TTCCTGCACT TCAGGAAAGG AATTACAGCA TTTACTTGAA CCATACGATG
    CTTCTGAAGG CAATACTCCT ACACTGTGGG ATCCCAGAGG ACAAGCTCAG CCAAGTCTAC
    GTCATTCTGT ATGATGCTGT GACAGAGAAG CTGACTAGGA GAGAAGTGGA AGCTAAATTC
    TGTAACCTGT CCTTGTCTTC TAATAGCCTG TGTAGACTCT ACAAGTTCAT TGAGCAGAAG
    GGGGATTTGC AAGACTTAAC GCCAACCATC AACTCACTAA TAAAACAGAA AACAGGCGTT
    GCTCAGTTGG TGAAGTATAG CTTAAAAGAC CTCGAGGACG TCGTCGGGCT GCTGAAGAAA
    CTTGGCGTGA AGTTACAGGT CTCCATCAAC CTGGGCTTGG TCTACAAGGT GCAGCAGCAC
    ACTGGCATCA TCTTCCAGTT CCTGGCATTC AGCAAACGCA GGCAAAGGGT TGTGCCTGAG
    ATCCTTGCCG CCGGTGGCAG ATACGACCTG CTGATTCCCA AGTTCAGAGG CCCACAGACT
    GTGGGGCCAG TCCCCACTGC TGTCGGTGTC AGCATAGCCA TAGACAAGAT ATTTGCTGCT
    GTCCTCAACA TGGAAGAGCC TGTTACAGTG AGCTCCTGTG ACCTCCTGGT TGTCAGTGTT
    GGCCAGATGT CCATGTCCAG GGCCATCAAC CTAACCCAGA AACTGTGGAC AGCGGGCATC
    ACTGCAGAAA TCATGTATGA CTGGTCCCAG TCCCAGGAAG AGTTACAAGA GTACTGCAGA
    CACCATGAAA TCACCTATGT GGCTCTGGTC TCCGATAAAG AAGGAAGCCA TGTCAAGGTC
    AAGTCCTTTG AGAAGGAGAG GCAAACAGAA AAGCGTGTAT TGGAATCGGA TCTTGTGGAT
    CACGTTATGC AGAAACTAAG AACCAAAGTT GGTGACGAAA GAAATTTCAG AGACGCTTCT
    GATAATCTTG CCGTACAAAC TCTGAAGGGG TCGTTTTCTA ATGCTTCAGG TTTGTTTGAA
    ATCCATGGAA CTACAGTAGT CCCCAACGTG ATTGTTCTAG CACCAGAAAA GCTGTCAGCC
    AGCACCAGGA GGCGACATGA GATTCAGGTG CAAACCCGAC TTCAGACTAC ACTTGCCAAC
    TTACATCAGA AAAGCAGTGA AATTGAAATT TTGGCTGTGG ACCTACCCAA GGAAACAATC
    TTACAGTTTC TATCATTAGA GTGGGATGCT GATGAACAGG CATTTAACAC AACTGTGAAG
    CAGCTGCTGT CACGCCTGCC AAAGCAAAGA TACCTCAAAC TCGTCTGCGA TGAAATTTAT
    AACATCAAAG TTGAAAAGAA GGTGTCAGTG CTGTTCCTGT ACAGCTACAG AGATGACTAC
    TACAGAATCC TATTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006499689.1
    CDS128..4705
    Translation

    Target ORF information:

    RefSeq Version XM_006499626.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006499626.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGCTGGGG GCCGCGGGGC CTCTGGACGC GGCCGGGCTG AGCCACAGGA GAGCTATTCG 
    CAGCGACAGG ACCACGAGCT GCAGGCCCTG GAGGCCATTT ACGGCTCGGA CTTCCAGGAT
    CTGCGGCCGG ATGCGCGCGG ACGGCAACGT GAAATCCTCC ACGAGATTCA GAGAAGGAAA
    GAGGAGATCA AGGAAGAGAA GAAAAGGAAG GAAATGGCTA AGCAGGAACG TTTGGAAATC
    ACTAGCTTGA CAAACCAGGA CTATGCCTCT AAGAGAGACC CAGCAGGGCA CAGGGCAGCT
    GCCATCCTCC ATGGAGGCTC TCCTGACTTT GTAGGAAATG GTAAAGCCCG GACATACTCC
    TCAGGAAGGT CCAGGCGAGA ACGTCAGTAT TCTGTGTGTA GTGGTGAACC CTCTCCTGGC
    TCGTGTGACA TCCTCCATTT CTCTGTGGGT AGCCCTGATC AGCTCATGGT GCACAAAGGG
    AGATGTGTTG GCAGTGATGA GCAGCTGGGA AAGGTGGTAT ACAATGCTTT GGAAACAGCC
    ACGGGCAGCT TTGTCTTGCT GCATGAGTGG GTCCTTCAAT GGCAGAAGAT GGGCCCTTGC
    CTTACCAGTC AAGAGAAAGA GAAGATTGAC AAGTGCAAAA GGCAGATTCA AGGAGCAGAA
    ACAGAATTCA GCTCCCTAGT GAAACTGAGC CATCCAAATA TCGTCCGCTA CTTTGCGATG
    AACTCCAGAG AGGAGGAGGA CTCCATTGTG ATCGACATTC TGGCAGAGCA CGTCAGTGGC
    ATCTCTCTGG CTACACACCT GAGCCACTCG GGCCCAGTCC CCGCCCACCA GCTGCGCAAG
    TACACAGCCC AGCTCCTGGC AGGCCTGGAT TACCTACACA GCAACTCCGT GGTGCACAAG
    GTTCTGAGCG CGTCCAGTGT CTTGGTAGAT GCCGAGGGCA CTGTCAAGAT AACAGACTAC
    AGCATCTCTA AGCGTCTGGC AGACATTTGC AAGGAGGATG TATTTGAGCA AGCTCGAGTT
    CGTTTTAGTG ACAGTGCCCT GCCTTATAAA ACAGGAAAGA AAGGGGATGT GTGGCGGCTC
    GGCCTCCTGC TGTTGTCTCT CAGCCAAGGA CAGGAGTGTG GGGAGTATCC GGTGACCATC
    CCCAGTGACC TGCCAGCTGA CTTCCAAGAC TTCCTGAAGA AGTGTGTCTG CCTGGACGAC
    AAGGAAAGAT GGAGCCCTCA GCAGCTGCTG AAACACAGCT TTATAAACCC TCAACCAAAG
    CTGCCTTTGG TGGAGCAGAG TCCTGAAGAC TCTGGGGGAC AAGACTACAT TGAGACCGTC
    ATTCCCAGCA ACCAGCTGCC CAGCGCTGCG TTCTTCAGTG AGACACAGAA ACAGTTCTCC
    CGGTACTTCA TTGAGTTTGA AGAACTACAA CTCCTAGGGA AAGGAGCTTT TGGAGCTGTC
    ATCAAGGTGC AAAACAAGCT TGATGGCTGC TGCTATGCTG TGAAGCGCAT CCCTATCAAC
    CCTGCAAGCA GACACTTCCG CCGGATCAAG GGCGAGGTGA CACTGCTATC GCGCCTGCAC
    CATGAGAACA TTGTGCGCTA CTACAACGCC TGGATCGAGC GGCATGAGCG CCCAGCGGTG
    CCAGGGACAC CGCCCCCAGA CTGCACACCC CAGGCCCAGG ACAGCCCAGC CACCTGCGGG
    AAAACATCAG GCGACACTGA AGAACTGGGC AGCGTGGAGG CTGCAGCACC GCCACCCATC
    CTCAGCAGCT CGGTGGAGTG GAGCACATCT GCAGAGCGTT CTACCAGCAC CCGCTTCCCA
    GTCACGGGCC AGGATTCCAG CAGCGATGAG GAGGACGAGG ACGAGCGCGA TGGCGTCTTC
    TCCCAGTCCT TTCTACCTGC TTCAGATTCT GACAGTGACA TCATCTTTGA CAATGAAGAT
    GAAAACAGTA AAAGTCAAAA TCAGGATGAA GACTGCAATC AAAAGGACGG TAGCCATGAG
    ATTGAGCCTT CAGTGACAGC TGAGGCTGTG CACTACCTTT ATATCCAGAT GGAGTACTGC
    GAGAAGAGCA CGCTGCGGGA CACCATTGAC CAGGGGCTGT TCCGAGACAC CAGCCGGCTC
    TGGAGGCTTT TCCGAGAGAT TCTGGATGGA TTAGCTTATA TCCATGAGAA AGGAATGATT
    CATCGCGACT TGAAGCCTGT CAATATTTTT TTGGATTCTG ATGACCATGT GAAAATAGGT
    GACTTTGGCC TGGCAACAGA CCATCTGGCC TTCACTGCTG AAGGTAAACA GGACGACCAG
    GCAGGTGACG GAGTGATTAA GTCAGACCCT TCAGGCCATT TGACTGGCAT GGTTGGTACT
    GCTCTGTATG TAAGCCCTGA GGTCCAAGGA AGCACCAAGT CTGCATACAA CCAGAAAGTG
    GATCTCTTCA GCCTGGGAAT TATCTTCTTT GAGATGTCTT ATCACCCGAT GGTGACTGCC
    TCAGAAAGAA TTTTCGTTCT CAACCAACTT CGAGATCCCA CATCGCCCAA GTTTCCAGAC
    GACTTCGATG ATGGAGAACA TACAAAGCAG AAATCTGTCA TCTCCTGGCT GTTGAACCAT
    GATCCAGCTA AACGGCCCAC GGCCATGGAA CTGCTCAAGA GTGAGCTGCT CCCCCCGCCG
    CAGATGGAGG AGTCCGAGCT GCATGAAGTG CTGCACCACA CGCTGGCCAA CATAGATGGG
    AAGGCCTACC GCACCATGAT GAGTCAGATC TTCTGCCAGC ACATCTCCCC CGCTATAGAC
    TACACCTACG ACAGTGACAT CCTGAAGGGC AACTTCTTGA TTCGTACAGC CAAGATCCAG
    CAGCTTGTAT GTGAAACCAT CGTCCGCGTC TTTAAAAGGC ATGGCGCTGT CCAGCTGTGC
    ACCCCACTGC TGCTTCCCCG AAACAGGCAA ATATATGAGC ACAACGAAGC GGCTTTGTTC
    ATGGATCACA GCGGCATGCT GGTGATGCTT CCGTTCGACC TGCGGGTCCC TTTTGCAAGA
    TATGTGGCAA GAAATAACAT ACTGAATTTA AAACGATACT GCATAGAGCG GGTGTTCAGA
    CCTCGGAAGT TAGACCGATT TCATCCCAAA GAACTCCTGG AATGTGCATT TGATATCGTC
    ACTTCTACCA CCAACAGCTC TCTGCCCACT GCCGAAACCA TCTACACCAT CTATGAAATA
    ATCCAGGAGT TTCCTGCACT TCAGGAAAGG AATTACAGCA TTTACTTGAA CCATACGATG
    CTTCTGAAGG CAATACTCCT ACACTGTGGG ATCCCAGAGG ACAAGCTCAG CCAAGTCTAC
    GTCATTCTGT ATGATGCTGT GACAGAGAAG CTGACTAGGA GAGAAGTGGA AGCTAAATTC
    TGTAACCTGT CCTTGTCTTC TAATAGCCTG TGTAGACTCT ACAAGTTCAT TGAGCAGAAG
    GGGGATTTGC AAGACTTAAC GCCAACCATC AACTCACTAA TAAAACAGAA AACAGGCGTT
    GCTCAGTTGG TGAAGTATAG CTTAAAAGAC CTCGAGGACG TCGTCGGGCT GCTGAAGAAA
    CTTGGCGTGA AGTTACAGGT CTCCATCAAC CTGGGCTTGG TCTACAAGGT GCAGCAGCAC
    ACTGGCATCA TCTTCCAGTT CCTGGCATTC AGCAAACGCA GGCAAAGGGT TGTGCCTGAG
    ATCCTTGCCG CCGGTGGCAG ATACGACCTG CTGATTCCCA AGTTCAGAGG CCCACAGACT
    GTGGGGCCAG TCCCCACTGC TGTCGGTGTC AGCATAGCCA TAGACAAGAT ATTTGCTGCT
    GTCCTCAACA TGGAAGAGCC TGTTACAGTG AGCTCCTGTG ACCTCCTGGT TGTCAGTGTT
    GGCCAGATGT CCATGTCCAG GGCCATCAAC CTAACCCAGA AACTGTGGAC AGCGGGCATC
    ACTGCAGAAA TCATGTATGA CTGGTCCCAG TCCCAGGAAG AGTTACAAGA GTACTGCAGA
    CACCATGAAA TCACCTATGT GGCTCTGGTC TCCGATAAAG AAGGAAGCCA TGTCAAGGTC
    AAGTCCTTTG AGAAGGAGAG GCAAACAGAA AAGCGTGTAT TGGAATCGGA TCTTGTGGAT
    CACGTTATGC AGAAACTAAG AACCAAAGTT GGTGACGAAA GAAATTTCAG AGACGCTTCT
    GATAATCTTG CCGTACAAAC TCTGAAGGGG TCGTTTTCTA ATGCTTCAGG TTTGTTTGAA
    ATCCATGGAA CTACAGTAGT CCCCAACGTG ATTGTTCTAG CACCAGAAAA GCTGTCAGCC
    AGCACCAGGA GGCGACATGA GATTCAGGTG CAAACCCGAC TTCAGACTAC ACTTGCCAAC
    TTACATCAGA AAAGCAGTGA AATTGAAATT TTGGCTGTGG ACCTACCCAA GGAAACAATC
    TTACAGTTTC TATCATTAGA GTGGGATGCT GATGAACAGG CATTTAACAC AACTGTGAAG
    CAGCTGCTGT CACGCCTGCC AAAGCAAAGA TACCTCAAAC TCGTCTGCGA TGAAATTTAT
    AACATCAAAG TTGAAAAGAA GGTGTCAGTG CTGTTCCTGT ACAGCTACAG AGATGACTAC
    TACAGAATCC TATTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu30529
    Clone ID Related Accession (Same CDS sequence) XM_006499627.4 , XM_006499627.3
    Accession Version XM_006499627.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4497bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product eIF-2-alpha kinase GCN2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006499627.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCTGGGG GCCGCGGGGC CTCTGGACGC GGCCGGGCTG AGCCACAGGA GAGCTATTCG 
    CAGCGACAGG ACCACGAGCT GCAGGCCCTG GAGGCCATTT ACGGCTCGGA CTTCCAGGAT
    CTGCGGCCGG ATGCGCGCGG ACGGGAACGT TTGGAAATCA CTAGCTTGAC AAACCAGGAC
    TATGCCTCTA AGAGAGACCC AGCAGGGCAC AGGGCAGCTG CCATCCTCCA TGGAGGCTCT
    CCTGACTTTG TAGGAAATGG TAAAGCCCGG ACATACTCCT CAGGAAGGTC CAGGCGAGAA
    CGTCAGTATT CTGTGTGTAG TGGTGAACCC TCTCCTGGCT CGTGTGACAT CCTCCATTTC
    TCTGTGGGTA GCCCTGATCA GCTCATGGTG CACAAAGGGA GATGTGTTGG CAGTGATGAG
    CAGCTGGGAA AGGTGGTATA CAATGCTTTG GAAACAGCCA CGGGCAGCTT TGTCTTGCTG
    CATGAGTGGG TCCTTCAATG GCAGAAGATG GGCCCTTGCC TTACCAGTCA AGAGAAAGAG
    AAGATTGACA AGTGCAAAAG GCAGATTCAA GGAGCAGAAA CAGAATTCAG CTCCCTAGTG
    AAACTGAGCC ATCCAAATAT CGTCCGCTAC TTTGCGATGA ACTCCAGAGA GGAGGAGGAC
    TCCATTGTGA TCGACATTCT GGCAGAGCAC GTCAGTGGCA TCTCTCTGGC TACACACCTG
    AGCCACTCGG GCCCAGTCCC CGCCCACCAG CTGCGCAAGT ACACAGCCCA GCTCCTGGCA
    GGCCTGGATT ACCTACACAG CAACTCCGTG GTGCACAAGG TTCTGAGCGC GTCCAGTGTC
    TTGGTAGATG CCGAGGGCAC TGTCAAGATA ACAGACTACA GCATCTCTAA GCGTCTGGCA
    GACATTTGCA AGGAGGATGT ATTTGAGCAA GCTCGAGTTC GTTTTAGTGA CAGTGCCCTG
    CCTTATAAAA CAGGAAAGAA AGGGGATGTG TGGCGGCTCG GCCTCCTGCT GTTGTCTCTC
    AGCCAAGGAC AGGAGTGTGG GGAGTATCCG GTGACCATCC CCAGTGACCT GCCAGCTGAC
    TTCCAAGACT TCCTGAAGAA GTGTGTCTGC CTGGACGACA AGGAAAGATG GAGCCCTCAG
    CAGCTGCTGA AACACAGCTT TATAAACCCT CAACCAAAGC TGCCTTTGGT GGAGCAGAGT
    CCTGAAGACT CTGGGGGACA AGACTACATT GAGACCGTCA TTCCCAGCAA CCAGCTGCCC
    AGCGCTGCGT TCTTCAGTGA GACACAGAAA CAGTTCTCCC GGTACTTCAT TGAGTTTGAA
    GAACTACAAC TCCTAGGGAA AGGAGCTTTT GGAGCTGTCA TCAAGGTGCA AAACAAGCTT
    GATGGCTGCT GCTATGCTGT GAAGCGCATC CCTATCAACC CTGCAAGCAG ACACTTCCGC
    CGGATCAAGG GCGAGGTGAC ACTGCTATCG CGCCTGCACC ATGAGAACAT TGTGCGCTAC
    TACAACGCCT GGATCGAGCG GCATGAGCGC CCAGCGGTGC CAGGGACACC GCCCCCAGAC
    TGCACACCCC AGGCCCAGGA CAGCCCAGCC ACCTGCGGGA AAACATCAGG CGACACTGAA
    GAACTGGGCA GCGTGGAGGC TGCAGCACCG CCACCCATCC TCAGCAGCTC GGTGGAGTGG
    AGCACATCTG CAGAGCGTTC TACCAGCACC CGCTTCCCAG TCACGGGCCA GGATTCCAGC
    AGCGATGAGG AGGACGAGGA CGAGCGCGAT GGCGTCTTCT CCCAGTCCTT TCTACCTGCT
    TCAGATTCTG ACAGTGACAT CATCTTTGAC AATGAAGATG AAAACAGTAA AAGTCAAAAT
    CAGGATGAAG ACTGCAATCA AAAGGACGGT AGCCATGAGA TTGAGCCTTC AGTGACAGCT
    GAGGCTGTGC ACTACCTTTA TATCCAGATG GAGTACTGCG AGAAGAGCAC GCTGCGGGAC
    ACCATTGACC AGGGGCTGTT CCGAGACACC AGCCGGCTCT GGAGGCTTTT CCGAGAGATT
    CTGGATGGAT TAGCTTATAT CCATGAGAAA GGAATGATTC ATCGCGACTT GAAGCCTGTC
    AATATTTTTT TGGATTCTGA TGACCATGTG AAAATAGGTG ACTTTGGCCT GGCAACAGAC
    CATCTGGCCT TCACTGCTGA AGGTAAACAG GACGACCAGG CAGGTGACGG AGTGATTAAG
    TCAGACCCTT CAGGCCATTT GACTGGCATG GTTGGTACTG CTCTGTATGT AAGCCCTGAG
    GTCCAAGGAA GCACCAAGTC TGCATACAAC CAGAAAGTGG ATCTCTTCAG CCTGGGAATT
    ATCTTCTTTG AGATGTCTTA TCACCCGATG GTGACTGCCT CAGAAAGAAT TTTCGTTCTC
    AACCAACTTC GAGATCCCAC ATCGCCCAAG TTTCCAGACG ACTTCGATGA TGGAGAACAT
    ACAAAGCAGA AATCTGTCAT CTCCTGGCTG TTGAACCATG ATCCAGCTAA ACGGCCCACG
    GCCATGGAAC TGCTCAAGAG TGAGCTGCTC CCCCCGCCGC AGATGGAGGA GTCCGAGCTG
    CATGAAGTGC TGCACCACAC GCTGGCCAAC ATAGATGGGA AGGCCTACCG CACCATGATG
    AGTCAGATCT TCTGCCAGCA CATCTCCCCC GCTATAGACT ACACCTACGA CAGTGACATC
    CTGAAGGGCA ACTTCTTGAT TCGTACAGCC AAGATCCAGC AGCTTGTATG TGAAACCATC
    GTCCGCGTCT TTAAAAGGCA TGGCGCTGTC CAGCTGTGCA CCCCACTGCT GCTTCCCCGA
    AACAGGCAAA TATATGAGCA CAACGAAGCG GCTTTGTTCA TGGATCACAG CGGCATGCTG
    GTGATGCTTC CGTTCGACCT GCGGGTCCCT TTTGCAAGAT ATGTGGCAAG AAATAACATA
    CTGAATTTAA AACGATACTG CATAGAGCGG GTGTTCAGAC CTCGGAAGTT AGACCGATTT
    CATCCCAAAG AACTCCTGGA ATGTGCATTT GATATCGTCA CTTCTACCAC CAACAGCTCT
    CTGCCCACTG CCGAAACCAT CTACACCATC TATGAAATAA TCCAGGAGTT TCCTGCACTT
    CAGGAAAGGA ATTACAGCAT TTACTTGAAC CATACGATGC TTCTGAAGGC AATACTCCTA
    CACTGTGGGA TCCCAGAGGA CAAGCTCAGC CAAGTCTACG TCATTCTGTA TGATGCTGTG
    ACAGAGAAGC TGACTAGGAG AGAAGTGGAA GCTAAATTCT GTAACCTGTC CTTGTCTTCT
    AATAGCCTGT GTAGACTCTA CAAGTTCATT GAGCAGAAGG GGGATTTGCA AGACTTAACG
    CCAACCATCA ACTCACTAAT AAAACAGAAA ACAGGCGTTG CTCAGTTGGT GAAGTATAGC
    TTAAAAGACC TCGAGGACGT CGTCGGGCTG CTGAAGAAAC TTGGCGTGAA GTTACAGGTC
    TCCATCAACC TGGGCTTGGT CTACAAGGTG CAGCAGCACA CTGGCATCAT CTTCCAGTTC
    CTGGCATTCA GCAAACGCAG GCAAAGGGTT GTGCCTGAGA TCCTTGCCGC CGGTGGCAGA
    TACGACCTGC TGATTCCCAA GTTCAGAGGC CCACAGACTG TGGGGCCAGT CCCCACTGCT
    GTCGGTGTCA GCATAGCCAT AGACAAGATA TTTGCTGCTG TCCTCAACAT GGAAGAGCCT
    GTTACAGTGA GCTCCTGTGA CCTCCTGGTT GTCAGTGTTG GCCAGATGTC CATGTCCAGG
    GCCATCAACC TAACCCAGAA ACTGTGGACA GCGGGCATCA CTGCAGAAAT CATGTATGAC
    TGGTCCCAGT CCCAGGAAGA GTTACAAGAG TACTGCAGAC ACCATGAAAT CACCTATGTG
    GCTCTGGTCT CCGATAAAGA AGGAAGCCAT GTCAAGGTCA AGTCCTTTGA GAAGGAGAGG
    CAAACAGAAA AGCGTGTATT GGAATCGGAT CTTGTGGATC ACGTTATGCA GAAACTAAGA
    ACCAAAGTTG GTGACGAAAG AAATTTCAGA GACGCTTCTG ATAATCTTGC CGTACAAACT
    CTGAAGGGGT CGTTTTCTAA TGCTTCAGGT TTGTTTGAAA TCCATGGAAC TACAGTAGTC
    CCCAACGTGA TTGTTCTAGC ACCAGAAAAG CTGTCAGCCA GCACCAGGAG GCGACATGAG
    ATTCAGGTGC AAACCCGACT TCAGACTACA CTTGCCAACT TACATCAGAA AAGCAGTGAA
    ATTGAAATTT TGGCTGTGGA CCTACCCAAG GAAACAATCT TACAGTTTCT ATCATTAGAG
    TGGGATGCTG ATGAACAGGC ATTTAACACA ACTGTGAAGC AGCTGCTGTC ACGCCTGCCA
    AAGCAAAGAT ACCTCAAACT CGTCTGCGAT GAAATTTATA ACATCAAAGT TGAAAAGAAG
    GTGTCAGTGC TGTTCCTGTA CAGCTACAGA GATGACTACT ACAGAATCCT ATTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006499690.1
    CDS128..4624
    Translation

    Target ORF information:

    RefSeq Version XM_006499627.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006499627.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCTGGGG GCCGCGGGGC CTCTGGACGC GGCCGGGCTG AGCCACAGGA GAGCTATTCG 
    CAGCGACAGG ACCACGAGCT GCAGGCCCTG GAGGCCATTT ACGGCTCGGA CTTCCAGGAT
    CTGCGGCCGG ATGCGCGCGG ACGGGAACGT TTGGAAATCA CTAGCTTGAC AAACCAGGAC
    TATGCCTCTA AGAGAGACCC AGCAGGGCAC AGGGCAGCTG CCATCCTCCA TGGAGGCTCT
    CCTGACTTTG TAGGAAATGG TAAAGCCCGG ACATACTCCT CAGGAAGGTC CAGGCGAGAA
    CGTCAGTATT CTGTGTGTAG TGGTGAACCC TCTCCTGGCT CGTGTGACAT CCTCCATTTC
    TCTGTGGGTA GCCCTGATCA GCTCATGGTG CACAAAGGGA GATGTGTTGG CAGTGATGAG
    CAGCTGGGAA AGGTGGTATA CAATGCTTTG GAAACAGCCA CGGGCAGCTT TGTCTTGCTG
    CATGAGTGGG TCCTTCAATG GCAGAAGATG GGCCCTTGCC TTACCAGTCA AGAGAAAGAG
    AAGATTGACA AGTGCAAAAG GCAGATTCAA GGAGCAGAAA CAGAATTCAG CTCCCTAGTG
    AAACTGAGCC ATCCAAATAT CGTCCGCTAC TTTGCGATGA ACTCCAGAGA GGAGGAGGAC
    TCCATTGTGA TCGACATTCT GGCAGAGCAC GTCAGTGGCA TCTCTCTGGC TACACACCTG
    AGCCACTCGG GCCCAGTCCC CGCCCACCAG CTGCGCAAGT ACACAGCCCA GCTCCTGGCA
    GGCCTGGATT ACCTACACAG CAACTCCGTG GTGCACAAGG TTCTGAGCGC GTCCAGTGTC
    TTGGTAGATG CCGAGGGCAC TGTCAAGATA ACAGACTACA GCATCTCTAA GCGTCTGGCA
    GACATTTGCA AGGAGGATGT ATTTGAGCAA GCTCGAGTTC GTTTTAGTGA CAGTGCCCTG
    CCTTATAAAA CAGGAAAGAA AGGGGATGTG TGGCGGCTCG GCCTCCTGCT GTTGTCTCTC
    AGCCAAGGAC AGGAGTGTGG GGAGTATCCG GTGACCATCC CCAGTGACCT GCCAGCTGAC
    TTCCAAGACT TCCTGAAGAA GTGTGTCTGC CTGGACGACA AGGAAAGATG GAGCCCTCAG
    CAGCTGCTGA AACACAGCTT TATAAACCCT CAACCAAAGC TGCCTTTGGT GGAGCAGAGT
    CCTGAAGACT CTGGGGGACA AGACTACATT GAGACCGTCA TTCCCAGCAA CCAGCTGCCC
    AGCGCTGCGT TCTTCAGTGA GACACAGAAA CAGTTCTCCC GGTACTTCAT TGAGTTTGAA
    GAACTACAAC TCCTAGGGAA AGGAGCTTTT GGAGCTGTCA TCAAGGTGCA AAACAAGCTT
    GATGGCTGCT GCTATGCTGT GAAGCGCATC CCTATCAACC CTGCAAGCAG ACACTTCCGC
    CGGATCAAGG GCGAGGTGAC ACTGCTATCG CGCCTGCACC ATGAGAACAT TGTGCGCTAC
    TACAACGCCT GGATCGAGCG GCATGAGCGC CCAGCGGTGC CAGGGACACC GCCCCCAGAC
    TGCACACCCC AGGCCCAGGA CAGCCCAGCC ACCTGCGGGA AAACATCAGG CGACACTGAA
    GAACTGGGCA GCGTGGAGGC TGCAGCACCG CCACCCATCC TCAGCAGCTC GGTGGAGTGG
    AGCACATCTG CAGAGCGTTC TACCAGCACC CGCTTCCCAG TCACGGGCCA GGATTCCAGC
    AGCGATGAGG AGGACGAGGA CGAGCGCGAT GGCGTCTTCT CCCAGTCCTT TCTACCTGCT
    TCAGATTCTG ACAGTGACAT CATCTTTGAC AATGAAGATG AAAACAGTAA AAGTCAAAAT
    CAGGATGAAG ACTGCAATCA AAAGGACGGT AGCCATGAGA TTGAGCCTTC AGTGACAGCT
    GAGGCTGTGC ACTACCTTTA TATCCAGATG GAGTACTGCG AGAAGAGCAC GCTGCGGGAC
    ACCATTGACC AGGGGCTGTT CCGAGACACC AGCCGGCTCT GGAGGCTTTT CCGAGAGATT
    CTGGATGGAT TAGCTTATAT CCATGAGAAA GGAATGATTC ATCGCGACTT GAAGCCTGTC
    AATATTTTTT TGGATTCTGA TGACCATGTG AAAATAGGTG ACTTTGGCCT GGCAACAGAC
    CATCTGGCCT TCACTGCTGA AGGTAAACAG GACGACCAGG CAGGTGACGG AGTGATTAAG
    TCAGACCCTT CAGGCCATTT GACTGGCATG GTTGGTACTG CTCTGTATGT AAGCCCTGAG
    GTCCAAGGAA GCACCAAGTC TGCATACAAC CAGAAAGTGG ATCTCTTCAG CCTGGGAATT
    ATCTTCTTTG AGATGTCTTA TCACCCGATG GTGACTGCCT CAGAAAGAAT TTTCGTTCTC
    AACCAACTTC GAGATCCCAC ATCGCCCAAG TTTCCAGACG ACTTCGATGA TGGAGAACAT
    ACAAAGCAGA AATCTGTCAT CTCCTGGCTG TTGAACCATG ATCCAGCTAA ACGGCCCACG
    GCCATGGAAC TGCTCAAGAG TGAGCTGCTC CCCCCGCCGC AGATGGAGGA GTCCGAGCTG
    CATGAAGTGC TGCACCACAC GCTGGCCAAC ATAGATGGGA AGGCCTACCG CACCATGATG
    AGTCAGATCT TCTGCCAGCA CATCTCCCCC GCTATAGACT ACACCTACGA CAGTGACATC
    CTGAAGGGCA ACTTCTTGAT TCGTACAGCC AAGATCCAGC AGCTTGTATG TGAAACCATC
    GTCCGCGTCT TTAAAAGGCA TGGCGCTGTC CAGCTGTGCA CCCCACTGCT GCTTCCCCGA
    AACAGGCAAA TATATGAGCA CAACGAAGCG GCTTTGTTCA TGGATCACAG CGGCATGCTG
    GTGATGCTTC CGTTCGACCT GCGGGTCCCT TTTGCAAGAT ATGTGGCAAG AAATAACATA
    CTGAATTTAA AACGATACTG CATAGAGCGG GTGTTCAGAC CTCGGAAGTT AGACCGATTT
    CATCCCAAAG AACTCCTGGA ATGTGCATTT GATATCGTCA CTTCTACCAC CAACAGCTCT
    CTGCCCACTG CCGAAACCAT CTACACCATC TATGAAATAA TCCAGGAGTT TCCTGCACTT
    CAGGAAAGGA ATTACAGCAT TTACTTGAAC CATACGATGC TTCTGAAGGC AATACTCCTA
    CACTGTGGGA TCCCAGAGGA CAAGCTCAGC CAAGTCTACG TCATTCTGTA TGATGCTGTG
    ACAGAGAAGC TGACTAGGAG AGAAGTGGAA GCTAAATTCT GTAACCTGTC CTTGTCTTCT
    AATAGCCTGT GTAGACTCTA CAAGTTCATT GAGCAGAAGG GGGATTTGCA AGACTTAACG
    CCAACCATCA ACTCACTAAT AAAACAGAAA ACAGGCGTTG CTCAGTTGGT GAAGTATAGC
    TTAAAAGACC TCGAGGACGT CGTCGGGCTG CTGAAGAAAC TTGGCGTGAA GTTACAGGTC
    TCCATCAACC TGGGCTTGGT CTACAAGGTG CAGCAGCACA CTGGCATCAT CTTCCAGTTC
    CTGGCATTCA GCAAACGCAG GCAAAGGGTT GTGCCTGAGA TCCTTGCCGC CGGTGGCAGA
    TACGACCTGC TGATTCCCAA GTTCAGAGGC CCACAGACTG TGGGGCCAGT CCCCACTGCT
    GTCGGTGTCA GCATAGCCAT AGACAAGATA TTTGCTGCTG TCCTCAACAT GGAAGAGCCT
    GTTACAGTGA GCTCCTGTGA CCTCCTGGTT GTCAGTGTTG GCCAGATGTC CATGTCCAGG
    GCCATCAACC TAACCCAGAA ACTGTGGACA GCGGGCATCA CTGCAGAAAT CATGTATGAC
    TGGTCCCAGT CCCAGGAAGA GTTACAAGAG TACTGCAGAC ACCATGAAAT CACCTATGTG
    GCTCTGGTCT CCGATAAAGA AGGAAGCCAT GTCAAGGTCA AGTCCTTTGA GAAGGAGAGG
    CAAACAGAAA AGCGTGTATT GGAATCGGAT CTTGTGGATC ACGTTATGCA GAAACTAAGA
    ACCAAAGTTG GTGACGAAAG AAATTTCAGA GACGCTTCTG ATAATCTTGC CGTACAAACT
    CTGAAGGGGT CGTTTTCTAA TGCTTCAGGT TTGTTTGAAA TCCATGGAAC TACAGTAGTC
    CCCAACGTGA TTGTTCTAGC ACCAGAAAAG CTGTCAGCCA GCACCAGGAG GCGACATGAG
    ATTCAGGTGC AAACCCGACT TCAGACTACA CTTGCCAACT TACATCAGAA AAGCAGTGAA
    ATTGAAATTT TGGCTGTGGA CCTACCCAAG GAAACAATCT TACAGTTTCT ATCATTAGAG
    TGGGATGCTG ATGAACAGGC ATTTAACACA ACTGTGAAGC AGCTGCTGTC ACGCCTGCCA
    AAGCAAAGAT ACCTCAAACT CGTCTGCGAT GAAATTTATA ACATCAAAGT TGAAAAGAAG
    GTGTCAGTGC TGTTCCTGTA CAGCTACAGA GATGACTACT ACAGAATCCT ATTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu30530
    Clone ID Related Accession (Same CDS sequence) XM_017318915.1 , NM_001355383.1
    Accession Version XM_017318915.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4377bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(House mouse)
    Product eIF-2-alpha kinase GCN2 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCCCACCT ACATACCCAG ATGTGAACGT TTGGAAATCA CTAGCTTGAC AAACCAGGAC 
    TATGCCTCTA AGAGAGACCC AGCAGGGCAC AGGGCAGCTG CCATCCTCCA TGGAGGCTCT
    CCTGACTTTG TAGGAAATGG TAAAGCCCGG ACATACTCCT CAGGAAGGTC CAGGCGAGAA
    CGTCAGTATT CTGTGTGTAG TGGTGAACCC TCTCCTGGCT CGTGTGACAT CCTCCATTTC
    TCTGTGGGTA GCCCTGATCA GCTCATGGTG CACAAAGGGA GATGTGTTGG CAGTGATGAG
    CAGCTGGGAA AGGTGGTATA CAATGCTTTG GAAACAGCCA CGGGCAGCTT TGTCTTGCTG
    CATGAGTGGG TCCTTCAATG GCAGAAGATG GGCCCTTGCC TTACCAGTCA AGAGAAAGAG
    AAGATTGACA AGTGCAAAAG GCAGATTCAA GGAGCAGAAA CAGAATTCAG CTCCCTAGTG
    AAACTGAGCC ATCCAAATAT CGTCCGCTAC TTTGCGATGA ACTCCAGAGA GGAGGAGGAC
    TCCATTGTGA TCGACATTCT GGCAGAGCAC GTCAGTGGCA TCTCTCTGGC TACACACCTG
    AGCCACTCGG GCCCAGTCCC CGCCCACCAG CTGCGCAAGT ACACAGCCCA GCTCCTGGCA
    GGCCTGGATT ACCTACACAG CAACTCCGTG GTGCACAAGG TTCTGAGCGC GTCCAGTGTC
    TTGGTAGATG CCGAGGGCAC TGTCAAGATA ACAGACTACA GCATCTCTAA GCGTCTGGCA
    GACATTTGCA AGGAGGATGT ATTTGAGCAA GCTCGAGTTC GTTTTAGTGA CAGTGCCCTG
    CCTTATAAAA CAGGAAAGAA AGGGGATGTG TGGCGGCTCG GCCTCCTGCT GTTGTCTCTC
    AGCCAAGGAC AGGAGTGTGG GGAGTATCCG GTGACCATCC CCAGTGACCT GCCAGCTGAC
    TTCCAAGACT TCCTGAAGAA GTGTGTCTGC CTGGACGACA AGGAAAGATG GAGCCCTCAG
    CAGCTGCTGA AACACAGCTT TATAAACCCT CAACCAAAGC TGCCTTTGGT GGAGCAGAGT
    CCTGAAGACT CTGGGGGACA AGACTACATT GAGACCGTCA TTCCCAGCAA CCAGCTGCCC
    AGCGCTGCGT TCTTCAGTGA GACACAGAAA CAGTTCTCCC GGTACTTCAT TGAGTTTGAA
    GAACTACAAC TCCTAGGGAA AGGAGCTTTT GGAGCTGTCA TCAAGGTGCA AAACAAGCTT
    GATGGCTGCT GCTATGCTGT GAAGCGCATC CCTATCAACC CTGCAAGCAG ACACTTCCGC
    CGGATCAAGG GCGAGGTGAC ACTGCTATCG CGCCTGCACC ATGAGAACAT TGTGCGCTAC
    TACAACGCCT GGATCGAGCG GCATGAGCGC CCAGCGGTGC CAGGGACACC GCCCCCAGAC
    TGCACACCCC AGGCCCAGGA CAGCCCAGCC ACCTGCGGGA AAACATCAGG CGACACTGAA
    GAACTGGGCA GCGTGGAGGC TGCAGCACCG CCACCCATCC TCAGCAGCTC GGTGGAGTGG
    AGCACATCTG CAGAGCGTTC TACCAGCACC CGCTTCCCAG TCACGGGCCA GGATTCCAGC
    AGCGATGAGG AGGACGAGGA CGAGCGCGAT GGCGTCTTCT CCCAGTCCTT TCTACCTGCT
    TCAGATTCTG ACAGTGACAT CATCTTTGAC AATGAAGATG AAAACAGTAA AAGTCAAAAT
    CAGGATGAAG ACTGCAATCA AAAGGACGGT AGCCATGAGA TTGAGCCTTC AGTGACAGCT
    GAGGCTGTGC ACTACCTTTA TATCCAGATG GAGTACTGCG AGAAGAGCAC GCTGCGGGAC
    ACCATTGACC AGGGGCTGTT CCGAGACACC AGCCGGCTCT GGAGGCTTTT CCGAGAGATT
    CTGGATGGAT TAGCTTATAT CCATGAGAAA GGAATGATTC ATCGCGACTT GAAGCCTGTC
    AATATTTTTT TGGATTCTGA TGACCATGTG AAAATAGGTG ACTTTGGCCT GGCAACAGAC
    CATCTGGCCT TCACTGCTGA AGGTAAACAG GACGACCAGG CAGGTGACGG AGTGATTAAG
    TCAGACCCTT CAGGCCATTT GACTGGCATG GTTGGTACTG CTCTGTATGT AAGCCCTGAG
    GTCCAAGGAA GCACCAAGTC TGCATACAAC CAGAAAGTGG ATCTCTTCAG CCTGGGAATT
    ATCTTCTTTG AGATGTCTTA TCACCCGATG GTGACTGCCT CAGAAAGAAT TTTCGTTCTC
    AACCAACTTC GAGATCCCAC ATCGCCCAAG TTTCCAGACG ACTTCGATGA TGGAGAACAT
    ACAAAGCAGA AATCTGTCAT CTCCTGGCTG TTGAACCATG ATCCAGCTAA ACGGCCCACG
    GCCATGGAAC TGCTCAAGAG TGAGCTGCTC CCCCCGCCGC AGATGGAGGA GTCCGAGCTG
    CATGAAGTGC TGCACCACAC GCTGGCCAAC ATAGATGGGA AGGCCTACCG CACCATGATG
    AGTCAGATCT TCTGCCAGCA CATCTCCCCC GCTATAGACT ACACCTACGA CAGTGACATC
    CTGAAGGGCA ACTTCTTGAT TCGTACAGCC AAGATCCAGC AGCTTGTATG TGAAACCATC
    GTCCGCGTCT TTAAAAGGCA TGGCGCTGTC CAGCTGTGCA CCCCACTGCT GCTTCCCCGA
    AACAGGCAAA TATATGAGCA CAACGAAGCG GCTTTGTTCA TGGATCACAG CGGCATGCTG
    GTGATGCTTC CGTTCGACCT GCGGGTCCCT TTTGCAAGAT ATGTGGCAAG AAATAACATA
    CTGAATTTAA AACGATACTG CATAGAGCGG GTGTTCAGAC CTCGGAAGTT AGACCGATTT
    CATCCCAAAG AACTCCTGGA ATGTGCATTT GATATCGTCA CTTCTACCAC CAACAGCTCT
    CTGCCCACTG CCGAAACCAT CTACACCATC TATGAAATAA TCCAGGAGTT TCCTGCACTT
    CAGGAAAGGA ATTACAGCAT TTACTTGAAC CATACGATGC TTCTGAAGGC AATACTCCTA
    CACTGTGGGA TCCCAGAGGA CAAGCTCAGC CAAGTCTACG TCATTCTGTA TGATGCTGTG
    ACAGAGAAGC TGACTAGGAG AGAAGTGGAA GCTAAATTCT GTAACCTGTC CTTGTCTTCT
    AATAGCCTGT GTAGACTCTA CAAGTTCATT GAGCAGAAGG GGGATTTGCA AGACTTAACG
    CCAACCATCA ACTCACTAAT AAAACAGAAA ACAGGCGTTG CTCAGTTGGT GAAGTATAGC
    TTAAAAGACC TCGAGGACGT CGTCGGGCTG CTGAAGAAAC TTGGCGTGAA GTTACAGGTC
    TCCATCAACC TGGGCTTGGT CTACAAGGTG CAGCAGCACA CTGGCATCAT CTTCCAGTTC
    CTGGCATTCA GCAAACGCAG GCAAAGGGTT GTGCCTGAGA TCCTTGCCGC CGGTGGCAGA
    TACGACCTGC TGATTCCCAA GTTCAGAGGC CCACAGACTG TGGGGCCAGT CCCCACTGCT
    GTCGGTGTCA GCATAGCCAT AGACAAGATA TTTGCTGCTG TCCTCAACAT GGAAGAGCCT
    GTTACAGTGA GCTCCTGTGA CCTCCTGGTT GTCAGTGTTG GCCAGATGTC CATGTCCAGG
    GCCATCAACC TAACCCAGAA ACTGTGGACA GCGGGCATCA CTGCAGAAAT CATGTATGAC
    TGGTCCCAGT CCCAGGAAGA GTTACAAGAG TACTGCAGAC ACCATGAAAT CACCTATGTG
    GCTCTGGTCT CCGATAAAGA AGGAAGCCAT GTCAAGGTCA AGTCCTTTGA GAAGGAGAGG
    CAAACAGAAA AGCGTGTATT GGAATCGGAT CTTGTGGATC ACGTTATGCA GAAACTAAGA
    ACCAAAGTTG GTGACGAAAG AAATTTCAGA GACGCTTCTG ATAATCTTGC CGTACAAACT
    CTGAAGGGGT CGTTTTCTAA TGCTTCAGGT TTGTTTGAAA TCCATGGAAC TACAGTAGTC
    CCCAACGTGA TTGTTCTAGC ACCAGAAAAG CTGTCAGCCA GCACCAGGAG GCGACATGAG
    ATTCAGGTGC AAACCCGACT TCAGACTACA CTTGCCAACT TACATCAGAA AAGCAGTGAA
    ATTGAAATTT TGGCTGTGGA CCTACCCAAG GAAACAATCT TACAGTTTCT ATCATTAGAG
    TGGGATGCTG ATGAACAGGC ATTTAACACA ACTGTGAAGC AGCTGCTGTC ACGCCTGCCA
    AAGCAAAGAT ACCTCAAACT CGTCTGCGAT GAAATTTATA ACATCAAAGT TGAAAAGAAG
    GTGTCAGTGC TGTTCCTGTA CAGCTACAGA GATGACTACT ACAGAATCCT ATTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017174404.1
    CDS286..4662
    Translation

    Target ORF information:

    RefSeq Version XM_017318915.1
    Organism Mus musculus(House mouse)
    Definition Mus musculus eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017318915.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCCCACCT ACATACCCAG ATGTGAACGT TTGGAAATCA CTAGCTTGAC AAACCAGGAC 
    TATGCCTCTA AGAGAGACCC AGCAGGGCAC AGGGCAGCTG CCATCCTCCA TGGAGGCTCT
    CCTGACTTTG TAGGAAATGG TAAAGCCCGG ACATACTCCT CAGGAAGGTC CAGGCGAGAA
    CGTCAGTATT CTGTGTGTAG TGGTGAACCC TCTCCTGGCT CGTGTGACAT CCTCCATTTC
    TCTGTGGGTA GCCCTGATCA GCTCATGGTG CACAAAGGGA GATGTGTTGG CAGTGATGAG
    CAGCTGGGAA AGGTGGTATA CAATGCTTTG GAAACAGCCA CGGGCAGCTT TGTCTTGCTG
    CATGAGTGGG TCCTTCAATG GCAGAAGATG GGCCCTTGCC TTACCAGTCA AGAGAAAGAG
    AAGATTGACA AGTGCAAAAG GCAGATTCAA GGAGCAGAAA CAGAATTCAG CTCCCTAGTG
    AAACTGAGCC ATCCAAATAT CGTCCGCTAC TTTGCGATGA ACTCCAGAGA GGAGGAGGAC
    TCCATTGTGA TCGACATTCT GGCAGAGCAC GTCAGTGGCA TCTCTCTGGC TACACACCTG
    AGCCACTCGG GCCCAGTCCC CGCCCACCAG CTGCGCAAGT ACACAGCCCA GCTCCTGGCA
    GGCCTGGATT ACCTACACAG CAACTCCGTG GTGCACAAGG TTCTGAGCGC GTCCAGTGTC
    TTGGTAGATG CCGAGGGCAC TGTCAAGATA ACAGACTACA GCATCTCTAA GCGTCTGGCA
    GACATTTGCA AGGAGGATGT ATTTGAGCAA GCTCGAGTTC GTTTTAGTGA CAGTGCCCTG
    CCTTATAAAA CAGGAAAGAA AGGGGATGTG TGGCGGCTCG GCCTCCTGCT GTTGTCTCTC
    AGCCAAGGAC AGGAGTGTGG GGAGTATCCG GTGACCATCC CCAGTGACCT GCCAGCTGAC
    TTCCAAGACT TCCTGAAGAA GTGTGTCTGC CTGGACGACA AGGAAAGATG GAGCCCTCAG
    CAGCTGCTGA AACACAGCTT TATAAACCCT CAACCAAAGC TGCCTTTGGT GGAGCAGAGT
    CCTGAAGACT CTGGGGGACA AGACTACATT GAGACCGTCA TTCCCAGCAA CCAGCTGCCC
    AGCGCTGCGT TCTTCAGTGA GACACAGAAA CAGTTCTCCC GGTACTTCAT TGAGTTTGAA
    GAACTACAAC TCCTAGGGAA AGGAGCTTTT GGAGCTGTCA TCAAGGTGCA AAACAAGCTT
    GATGGCTGCT GCTATGCTGT GAAGCGCATC CCTATCAACC CTGCAAGCAG ACACTTCCGC
    CGGATCAAGG GCGAGGTGAC ACTGCTATCG CGCCTGCACC ATGAGAACAT TGTGCGCTAC
    TACAACGCCT GGATCGAGCG GCATGAGCGC CCAGCGGTGC CAGGGACACC GCCCCCAGAC
    TGCACACCCC AGGCCCAGGA CAGCCCAGCC ACCTGCGGGA AAACATCAGG CGACACTGAA
    GAACTGGGCA GCGTGGAGGC TGCAGCACCG CCACCCATCC TCAGCAGCTC GGTGGAGTGG
    AGCACATCTG CAGAGCGTTC TACCAGCACC CGCTTCCCAG TCACGGGCCA GGATTCCAGC
    AGCGATGAGG AGGACGAGGA CGAGCGCGAT GGCGTCTTCT CCCAGTCCTT TCTACCTGCT
    TCAGATTCTG ACAGTGACAT CATCTTTGAC AATGAAGATG AAAACAGTAA AAGTCAAAAT
    CAGGATGAAG ACTGCAATCA AAAGGACGGT AGCCATGAGA TTGAGCCTTC AGTGACAGCT
    GAGGCTGTGC ACTACCTTTA TATCCAGATG GAGTACTGCG AGAAGAGCAC GCTGCGGGAC
    ACCATTGACC AGGGGCTGTT CCGAGACACC AGCCGGCTCT GGAGGCTTTT CCGAGAGATT
    CTGGATGGAT TAGCTTATAT CCATGAGAAA GGAATGATTC ATCGCGACTT GAAGCCTGTC
    AATATTTTTT TGGATTCTGA TGACCATGTG AAAATAGGTG ACTTTGGCCT GGCAACAGAC
    CATCTGGCCT TCACTGCTGA AGGTAAACAG GACGACCAGG CAGGTGACGG AGTGATTAAG
    TCAGACCCTT CAGGCCATTT GACTGGCATG GTTGGTACTG CTCTGTATGT AAGCCCTGAG
    GTCCAAGGAA GCACCAAGTC TGCATACAAC CAGAAAGTGG ATCTCTTCAG CCTGGGAATT
    ATCTTCTTTG AGATGTCTTA TCACCCGATG GTGACTGCCT CAGAAAGAAT TTTCGTTCTC
    AACCAACTTC GAGATCCCAC ATCGCCCAAG TTTCCAGACG ACTTCGATGA TGGAGAACAT
    ACAAAGCAGA AATCTGTCAT CTCCTGGCTG TTGAACCATG ATCCAGCTAA ACGGCCCACG
    GCCATGGAAC TGCTCAAGAG TGAGCTGCTC CCCCCGCCGC AGATGGAGGA GTCCGAGCTG
    CATGAAGTGC TGCACCACAC GCTGGCCAAC ATAGATGGGA AGGCCTACCG CACCATGATG
    AGTCAGATCT TCTGCCAGCA CATCTCCCCC GCTATAGACT ACACCTACGA CAGTGACATC
    CTGAAGGGCA ACTTCTTGAT TCGTACAGCC AAGATCCAGC AGCTTGTATG TGAAACCATC
    GTCCGCGTCT TTAAAAGGCA TGGCGCTGTC CAGCTGTGCA CCCCACTGCT GCTTCCCCGA
    AACAGGCAAA TATATGAGCA CAACGAAGCG GCTTTGTTCA TGGATCACAG CGGCATGCTG
    GTGATGCTTC CGTTCGACCT GCGGGTCCCT TTTGCAAGAT ATGTGGCAAG AAATAACATA
    CTGAATTTAA AACGATACTG CATAGAGCGG GTGTTCAGAC CTCGGAAGTT AGACCGATTT
    CATCCCAAAG AACTCCTGGA ATGTGCATTT GATATCGTCA CTTCTACCAC CAACAGCTCT
    CTGCCCACTG CCGAAACCAT CTACACCATC TATGAAATAA TCCAGGAGTT TCCTGCACTT
    CAGGAAAGGA ATTACAGCAT TTACTTGAAC CATACGATGC TTCTGAAGGC AATACTCCTA
    CACTGTGGGA TCCCAGAGGA CAAGCTCAGC CAAGTCTACG TCATTCTGTA TGATGCTGTG
    ACAGAGAAGC TGACTAGGAG AGAAGTGGAA GCTAAATTCT GTAACCTGTC CTTGTCTTCT
    AATAGCCTGT GTAGACTCTA CAAGTTCATT GAGCAGAAGG GGGATTTGCA AGACTTAACG
    CCAACCATCA ACTCACTAAT AAAACAGAAA ACAGGCGTTG CTCAGTTGGT GAAGTATAGC
    TTAAAAGACC TCGAGGACGT CGTCGGGCTG CTGAAGAAAC TTGGCGTGAA GTTACAGGTC
    TCCATCAACC TGGGCTTGGT CTACAAGGTG CAGCAGCACA CTGGCATCAT CTTCCAGTTC
    CTGGCATTCA GCAAACGCAG GCAAAGGGTT GTGCCTGAGA TCCTTGCCGC CGGTGGCAGA
    TACGACCTGC TGATTCCCAA GTTCAGAGGC CCACAGACTG TGGGGCCAGT CCCCACTGCT
    GTCGGTGTCA GCATAGCCAT AGACAAGATA TTTGCTGCTG TCCTCAACAT GGAAGAGCCT
    GTTACAGTGA GCTCCTGTGA CCTCCTGGTT GTCAGTGTTG GCCAGATGTC CATGTCCAGG
    GCCATCAACC TAACCCAGAA ACTGTGGACA GCGGGCATCA CTGCAGAAAT CATGTATGAC
    TGGTCCCAGT CCCAGGAAGA GTTACAAGAG TACTGCAGAC ACCATGAAAT CACCTATGTG
    GCTCTGGTCT CCGATAAAGA AGGAAGCCAT GTCAAGGTCA AGTCCTTTGA GAAGGAGAGG
    CAAACAGAAA AGCGTGTATT GGAATCGGAT CTTGTGGATC ACGTTATGCA GAAACTAAGA
    ACCAAAGTTG GTGACGAAAG AAATTTCAGA GACGCTTCTG ATAATCTTGC CGTACAAACT
    CTGAAGGGGT CGTTTTCTAA TGCTTCAGGT TTGTTTGAAA TCCATGGAAC TACAGTAGTC
    CCCAACGTGA TTGTTCTAGC ACCAGAAAAG CTGTCAGCCA GCACCAGGAG GCGACATGAG
    ATTCAGGTGC AAACCCGACT TCAGACTACA CTTGCCAACT TACATCAGAA AAGCAGTGAA
    ATTGAAATTT TGGCTGTGGA CCTACCCAAG GAAACAATCT TACAGTTTCT ATCATTAGAG
    TGGGATGCTG ATGAACAGGC ATTTAACACA ACTGTGAAGC AGCTGCTGTC ACGCCTGCCA
    AAGCAAAGAT ACCTCAAACT CGTCTGCGAT GAAATTTATA ACATCAAAGT TGAAAAGAAG
    GTGTCAGTGC TGTTCCTGTA CAGCTACAGA GATGACTACT ACAGAATCCT ATTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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