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BBC3 cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol BBC3
Entrez Gene ID 27113
Full Name BCL2 binding component 3
Synonyms JFY-1,JFY1,PUMA
General protein information
Preferred Names

BCL2 binding component 3

Names

bcl-2-binding component 3
p53 up-regulated modulator of apoptosis
p53-upregulated modulator of apoptosis
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

19

19q13.32
Summary This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011].
Disorder MIM:

605854


Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001127242.2 NP_001120714.1 bcl-2-binding component 3 isoform 3
NM_014417.5 NP_055232.1 bcl-2-binding component 3 isoform 4
XM_011526722.2 XP_011525024.1 bcl-2-binding component 3 isoform X1
XM_006723141.3 XP_006723204.1 bcl-2-binding component 3 isoform X2
NM_001127242.3 NP_001120714.1 bcl-2-binding component 3 isoform 3
NM_001127240.3 NP_001120712.1 bcl-2-binding component 3 isoform 1
NM_001127241.3 NP_001120713.1 bcl-2-binding component 3 isoform 2
NM_001127241.2 NP_001120713.1 bcl-2-binding component 3 isoform 2
XM_006723141.2 XP_006723204.1 bcl-2-binding component 3 isoform X1
NM_014417.4 NP_055232.1 bcl-2-binding component 3 isoform 4
NM_001127240.2 NP_001120712.1 bcl-2-binding component 3 isoform 1

Pathways from BioSystems


Homologies


Homo sapiens (human) BBC3 NP_055232.1
Pan troglodytes (chimpanzee) BBC3 XP_001168815.1
Rattus norvegicus (Norway rat) Bbc3 NP_776209.1
Canis lupus familiaris (dog) BBC3 XP_005616461.1
Mus musculus (house mouse) Bbc3 NP_573497.1

Gene Ontology

The following BBC3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BBC3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu19952 NM_014417.5
Latest version!		 Homo sapiens BCL2 binding component 3 (BBC3), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu19952 XM_006723141.3
Latest version!		 Homo sapiens BCL2 binding component 3 (BBC3), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu20051 NM_001127242.3
Latest version!		 Homo sapiens BCL2 binding component 3 (BBC3), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu19976 NM_001127240.3
Latest version!		 Homo sapiens BCL2 binding component 3 (BBC3), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $188.30
$269.00
OHu20134 NM_001127241.3
Latest version!		 Homo sapiens BCL2 binding component 3 (BBC3), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $69.30
$99.00
OHu67065 XM_011526722.2
Latest version!		 Homo sapiens BCL2 binding component 3 (BBC3), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $139.30
$199.00
View Old Accession Versions >>
OHu20051 NM_001127242.2 Homo sapiens BCL2 binding component 3 (BBC3), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu20134 NM_001127241.2 Homo sapiens BCL2 binding component 3 (BBC3), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $69.30
$99.00
OHu19952 XM_006723141.2 Homo sapiens BCL2 binding component 3 (BBC3), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu19952 NM_014417.4 Homo sapiens BCL2 binding component 3 (BBC3), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu19976 NM_001127240.2 Homo sapiens BCL2 binding component 3 (BBC3), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $188.30
$269.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu19952
Clone ID Related Accession (Same CDS sequence) NM_014417.5 , XM_006723141.3 , XM_006723141.2 , NM_014417.4
Accession Version NM_014417.5 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 582bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-19
Organism Homo sapiens(human)
Product bcl-2-binding component 3 isoform 4
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008532.7. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_014417.4. Transcript Variant: This variant (4) has alternate exon structure at its 5' end and it thus differs in the 5' UTR and 5' coding region, compared to variant 2. The encoded isoform (4, also known as PUMA-alpha) has a distinct N-terminus and is longer than isoform 2. Isoform 4 also includes the C-terminal BH3 domain and can localize to the mitochondria. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: ERR279869.944.1, DRR138517.347682.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02400288, SAMN02400289 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 11463392 RefSeq Select criteria :: based on manual assertion, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

1
61
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ATGGCCCGCG CACGCCAGGA GGGCAGCTCC CCGGAGCCCG TAGAGGGCCT GGCCCGCGAC 
GGCCCGCGCC CCTTCCCGCT CGGCCGCCTG GTGCCCTCGG CAGTGTCCTG CGGCCTCTGC 
GAGCCCGGCC TGGCTGCCGC CCCCGCCGCC CCCACCCTGC TGCCCGCTGC CTACCTCTGC 
GCCCCCACCG CCCCACCCGC CGTCACCGCC GCCCTGGGGG GTTCCCGCTG GCCTGGGGGT 
CCCCGCAGCC GGCCCCGAGG CCCGCGCCCG GACGGTCCTC AGCCCTCGCT CTCGCTGGCG 
GAGCAGCACC TGGAGTCGCC CGTGCCCAGC GCCCCGGGGG CTCTGGCGGG CGGTCCCACC 
CAGGCGGCCC CGGGAGTCCG CGGGGAGGAG GAACAGTGGG CCCGGGAGAT CGGGGCCCAG 
CTGCGGCGGA TGGCGGACGA CCTCAACGCA CAGTACGAGC GGCGGAGACA AGAGGAGCAG 
CAGCGGCACC GCCCCTCACC CTGGAGGGTC CTGTACAATC TCATCATGGG ACTCCTGCCC 
TTACCCAGGG GCCACAGAGC CCCCGAGATG GAGCCCAATT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_055232.1
CDS287..868
Translation

Target ORF information:

RefSeq Version NM_014417.5
Organism Homo sapiens(human)
Definition Homo sapiens BCL2 binding component 3 (BBC3), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_014417.5

ORF Insert Sequence:

1
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181
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481
541
ATGGCCCGCG CACGCCAGGA GGGCAGCTCC CCGGAGCCCG TAGAGGGCCT GGCCCGCGAC 
GGCCCGCGCC CCTTCCCGCT CGGCCGCCTG GTGCCCTCGG CAGTGTCCTG CGGCCTCTGC 
GAGCCCGGCC TGGCTGCCGC CCCCGCCGCC CCCACCCTGC TGCCCGCTGC CTACCTCTGC 
GCCCCCACCG CCCCACCCGC CGTCACCGCC GCCCTGGGGG GTTCCCGCTG GCCTGGGGGT 
CCCCGCAGCC GGCCCCGAGG CCCGCGCCCG GACGGTCCTC AGCCCTCGCT CTCGCTGGCG 
GAGCAGCACC TGGAGTCGCC CGTGCCCAGC GCCCCGGGGG CTCTGGCGGG CGGTCCCACC 
CAGGCGGCCC CGGGAGTCCG CGGGGAGGAG GAACAGTGGG CCCGGGAGAT CGGGGCCCAG 
CTGCGGCGGA TGGCGGACGA CCTCAACGCA CAGTACGAGC GGCGGAGACA AGAGGAGCAG 
CAGCGGCACC GCCCCTCACC CTGGAGGGTC CTGTACAATC TCATCATGGG ACTCCTGCCC 
TTACCCAGGG GCCACAGAGC CCCCGAGATG GAGCCCAATT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19952
Clone ID Related Accession (Same CDS sequence) NM_014417.5 , XM_006723141.3 , XM_006723141.2 , NM_014417.4
Accession Version NM_014417.4 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 582bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-03-03
Organism Homo sapiens(human)
Product bcl-2-binding component 3 isoform 4
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF332558.1, BG258126.1, U82987.1 and AA887822.1. On Dec 30, 2011 this sequence version replaced NM_014417.3. Transcript Variant: This variant (4) has alternate exon structure at its 5' end and it thus differs in the 5' UTR and 5' coding region, compared to variant 2. The encoded isoform (4, also known as PUMA-alpha) has a distinct N-terminus and is longer than isoform 2. Isoform 4 also includes the C-terminal BH3 domain and can localize to the mitochondria. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF332558.1, ERR279869.944.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02400288, SAMN02400289 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 11463392 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
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481
541
ATGGCCCGCG CACGCCAGGA GGGCAGCTCC CCGGAGCCCG TAGAGGGCCT GGCCCGCGAC 
GGCCCGCGCC CCTTCCCGCT CGGCCGCCTG GTGCCCTCGG CAGTGTCCTG CGGCCTCTGC 
GAGCCCGGCC TGGCTGCCGC CCCCGCCGCC CCCACCCTGC TGCCCGCTGC CTACCTCTGC 
GCCCCCACCG CCCCACCCGC CGTCACCGCC GCCCTGGGGG GTTCCCGCTG GCCTGGGGGT 
CCCCGCAGCC GGCCCCGAGG CCCGCGCCCG GACGGTCCTC AGCCCTCGCT CTCGCTGGCG 
GAGCAGCACC TGGAGTCGCC CGTGCCCAGC GCCCCGGGGG CTCTGGCGGG CGGTCCCACC 
CAGGCGGCCC CGGGAGTCCG CGGGGAGGAG GAACAGTGGG CCCGGGAGAT CGGGGCCCAG 
CTGCGGCGGA TGGCGGACGA CCTCAACGCA CAGTACGAGC GGCGGAGACA AGAGGAGCAG 
CAGCGGCACC GCCCCTCACC CTGGAGGGTC CTGTACAATC TCATCATGGG ACTCCTGCCC 
TTACCCAGGG GCCACAGAGC CCCCGAGATG GAGCCCAATT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_055232.1
CDS282..863
Translation

Target ORF information:

RefSeq Version NM_014417.4
Organism Homo sapiens(human)
Definition Homo sapiens BCL2 binding component 3 (BBC3), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_014417.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGCCCGCG CACGCCAGGA GGGCAGCTCC CCGGAGCCCG TAGAGGGCCT GGCCCGCGAC 
GGCCCGCGCC CCTTCCCGCT CGGCCGCCTG GTGCCCTCGG CAGTGTCCTG CGGCCTCTGC 
GAGCCCGGCC TGGCTGCCGC CCCCGCCGCC CCCACCCTGC TGCCCGCTGC CTACCTCTGC 
GCCCCCACCG CCCCACCCGC CGTCACCGCC GCCCTGGGGG GTTCCCGCTG GCCTGGGGGT 
CCCCGCAGCC GGCCCCGAGG CCCGCGCCCG GACGGTCCTC AGCCCTCGCT CTCGCTGGCG 
GAGCAGCACC TGGAGTCGCC CGTGCCCAGC GCCCCGGGGG CTCTGGCGGG CGGTCCCACC 
CAGGCGGCCC CGGGAGTCCG CGGGGAGGAG GAACAGTGGG CCCGGGAGAT CGGGGCCCAG 
CTGCGGCGGA TGGCGGACGA CCTCAACGCA CAGTACGAGC GGCGGAGACA AGAGGAGCAG 
CAGCGGCACC GCCCCTCACC CTGGAGGGTC CTGTACAATC TCATCATGGG ACTCCTGCCC 
TTACCCAGGG GCCACAGAGC CCCCGAGATG GAGCCCAATT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19952
Clone ID Related Accession (Same CDS sequence) NM_014417.5 , XM_006723141.3 , XM_006723141.2 , NM_014417.4
Accession Version XM_006723141.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 582bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product bcl-2-binding component 3 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006723141.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

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ATGGCCCGCG CACGCCAGGA GGGCAGCTCC CCGGAGCCCG TAGAGGGCCT GGCCCGCGAC 
GGCCCGCGCC CCTTCCCGCT CGGCCGCCTG GTGCCCTCGG CAGTGTCCTG CGGCCTCTGC 
GAGCCCGGCC TGGCTGCCGC CCCCGCCGCC CCCACCCTGC TGCCCGCTGC CTACCTCTGC 
GCCCCCACCG CCCCACCCGC CGTCACCGCC GCCCTGGGGG GTTCCCGCTG GCCTGGGGGT 
CCCCGCAGCC GGCCCCGAGG CCCGCGCCCG GACGGTCCTC AGCCCTCGCT CTCGCTGGCG 
GAGCAGCACC TGGAGTCGCC CGTGCCCAGC GCCCCGGGGG CTCTGGCGGG CGGTCCCACC 
CAGGCGGCCC CGGGAGTCCG CGGGGAGGAG GAACAGTGGG CCCGGGAGAT CGGGGCCCAG 
CTGCGGCGGA TGGCGGACGA CCTCAACGCA CAGTACGAGC GGCGGAGACA AGAGGAGCAG 
CAGCGGCACC GCCCCTCACC CTGGAGGGTC CTGTACAATC TCATCATGGG ACTCCTGCCC 
TTACCCAGGG GCCACAGAGC CCCCGAGATG GAGCCCAATT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006723204.1
CDS233..814
Translation

Target ORF information:

RefSeq Version XM_006723141.3
Organism Homo sapiens(human)
Definition Homo sapiens BCL2 binding component 3 (BBC3), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006723141.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGCCCGCG CACGCCAGGA GGGCAGCTCC CCGGAGCCCG TAGAGGGCCT GGCCCGCGAC 
GGCCCGCGCC CCTTCCCGCT CGGCCGCCTG GTGCCCTCGG CAGTGTCCTG CGGCCTCTGC 
GAGCCCGGCC TGGCTGCCGC CCCCGCCGCC CCCACCCTGC TGCCCGCTGC CTACCTCTGC 
GCCCCCACCG CCCCACCCGC CGTCACCGCC GCCCTGGGGG GTTCCCGCTG GCCTGGGGGT 
CCCCGCAGCC GGCCCCGAGG CCCGCGCCCG GACGGTCCTC AGCCCTCGCT CTCGCTGGCG 
GAGCAGCACC TGGAGTCGCC CGTGCCCAGC GCCCCGGGGG CTCTGGCGGG CGGTCCCACC 
CAGGCGGCCC CGGGAGTCCG CGGGGAGGAG GAACAGTGGG CCCGGGAGAT CGGGGCCCAG 
CTGCGGCGGA TGGCGGACGA CCTCAACGCA CAGTACGAGC GGCGGAGACA AGAGGAGCAG 
CAGCGGCACC GCCCCTCACC CTGGAGGGTC CTGTACAATC TCATCATGGG ACTCCTGCCC 
TTACCCAGGG GCCACAGAGC CCCCGAGATG GAGCCCAATT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19952
Clone ID Related Accession (Same CDS sequence) NM_014417.5 , XM_006723141.3 , XM_006723141.2 , NM_014417.4
Accession Version XM_006723141.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 582bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-03-11
Organism Homo sapiens(Human)
Product bcl-2-binding component 3 isoform X1
Comment 

1
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181
241
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361
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ATGGCCCGCG CACGCCAGGA GGGCAGCTCC CCGGAGCCCG TAGAGGGCCT GGCCCGCGAC 
GGCCCGCGCC CCTTCCCGCT CGGCCGCCTG GTGCCCTCGG CAGTGTCCTG CGGCCTCTGC 
GAGCCCGGCC TGGCTGCCGC CCCCGCCGCC CCCACCCTGC TGCCCGCTGC CTACCTCTGC 
GCCCCCACCG CCCCACCCGC CGTCACCGCC GCCCTGGGGG GTTCCCGCTG GCCTGGGGGT 
CCCCGCAGCC GGCCCCGAGG CCCGCGCCCG GACGGTCCTC AGCCCTCGCT CTCGCTGGCG 
GAGCAGCACC TGGAGTCGCC CGTGCCCAGC GCCCCGGGGG CTCTGGCGGG CGGTCCCACC 
CAGGCGGCCC CGGGAGTCCG CGGGGAGGAG GAACAGTGGG CCCGGGAGAT CGGGGCCCAG 
CTGCGGCGGA TGGCGGACGA CCTCAACGCA CAGTACGAGC GGCGGAGACA AGAGGAGCAG 
CAGCGGCACC GCCCCTCACC CTGGAGGGTC CTGTACAATC TCATCATGGG ACTCCTGCCC 
TTACCCAGGG GCCACAGAGC CCCCGAGATG GAGCCCAATT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006723204.1
Translation

Target ORF information:

RefSeq Version XM_006723141.2
Organism Homo sapiens(Human)
Definition Homo sapiens BCL2 binding component 3 (BBC3), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006723141.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGCCCGCG CACGCCAGGA GGGCAGCTCC CCGGAGCCCG TAGAGGGCCT GGCCCGCGAC 
GGCCCGCGCC CCTTCCCGCT CGGCCGCCTG GTGCCCTCGG CAGTGTCCTG CGGCCTCTGC 
GAGCCCGGCC TGGCTGCCGC CCCCGCCGCC CCCACCCTGC TGCCCGCTGC CTACCTCTGC 
GCCCCCACCG CCCCACCCGC CGTCACCGCC GCCCTGGGGG GTTCCCGCTG GCCTGGGGGT 
CCCCGCAGCC GGCCCCGAGG CCCGCGCCCG GACGGTCCTC AGCCCTCGCT CTCGCTGGCG 
GAGCAGCACC TGGAGTCGCC CGTGCCCAGC GCCCCGGGGG CTCTGGCGGG CGGTCCCACC 
CAGGCGGCCC CGGGAGTCCG CGGGGAGGAG GAACAGTGGG CCCGGGAGAT CGGGGCCCAG 
CTGCGGCGGA TGGCGGACGA CCTCAACGCA CAGTACGAGC GGCGGAGACA AGAGGAGCAG 
CAGCGGCACC GCCCCTCACC CTGGAGGGTC CTGTACAATC TCATCATGGG ACTCCTGCCC 
TTACCCAGGG GCCACAGAGC CCCCGAGATG GAGCCCAATT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20051
Clone ID Related Accession (Same CDS sequence) NM_001127242.2 , NM_001127242.3
Accession Version NM_001127242.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 306bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-03-03
Organism Homo sapiens(human)
Product bcl-2-binding component 3 isoform 3
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF354656.1, BG258126.1, U82987.1 and AA887822.1. On Dec 30, 2011 this sequence version replaced NM_001127242.1. Transcript Variant: This variant (3) lacks two exons spanning the 5' and central coding regions, resulting in a frameshift in the 3' coding region, compared to variant 2. The encoded isoform (3, also known as PUMA-delta) has the same N-terminus but is otherwise distinct and shorter, compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF354656.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2151887 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 11463392 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

1
61
121
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241
301
ATGAAATTTG GCATGGGGTC TGCCCAGGCA TGTCCATGCC AGGTGCCCAG GGCTGCTTCC 
ACGACGTGGG TCCCCTGCCA GATTTGTGAG ACAAGAGGAG CAGCAGCGGC ACCGCCCCTC 
ACCCTGGAGG GTCCTGTACA ATCTCATCAT GGGACTCCTG CCCTTACCCA GGGGCCACAG 
AGCCCCCGAG ATGGAGCCCA ATTAGGTGCC TGCACCCGCC CGGTGGACGT CAGGGACTCG 
GGGGGCAGGC CCCTCCCACC TCCTGACACC CTGGCCAGCG CGGGGGACTT TCTCTGCACC 
ATGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001120714.1
CDS165..470
Translation

Target ORF information:

RefSeq Version NM_001127242.2
Organism Homo sapiens(human)
Definition Homo sapiens BCL2 binding component 3 (BBC3), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001127242.2

ORF Insert Sequence:

1
61
121
181
241
301
ATGAAATTTG GCATGGGGTC TGCCCAGGCA TGTCCATGCC AGGTGCCCAG GGCTGCTTCC 
ACGACGTGGG TCCCCTGCCA GATTTGTGAG ACAAGAGGAG CAGCAGCGGC ACCGCCCCTC 
ACCCTGGAGG GTCCTGTACA ATCTCATCAT GGGACTCCTG CCCTTACCCA GGGGCCACAG 
AGCCCCCGAG ATGGAGCCCA ATTAGGTGCC TGCACCCGCC CGGTGGACGT CAGGGACTCG 
GGGGGCAGGC CCCTCCCACC TCCTGACACC CTGGCCAGCG CGGGGGACTT TCTCTGCACC 
ATGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20051
Clone ID Related Accession (Same CDS sequence) NM_001127242.2 , NM_001127242.3
Accession Version NM_001127242.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 306bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2020-01-04
Organism Homo sapiens(human)
Product bcl-2-binding component 3 isoform 3
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008532.7. On Jun 2, 2019 this sequence version replaced NM_001127242.2. Transcript Variant: This variant (3) lacks two exons spanning the 5' and central coding regions, resulting in a frameshift in the 3' coding region, compared to variant 2. The encoded isoform (3, also known as PUMA-delta) has the same N-terminus but is otherwise distinct and shorter, compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF354656.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2151887 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 11463392 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
ATGAAATTTG GCATGGGGTC TGCCCAGGCA TGTCCATGCC AGGTGCCCAG GGCTGCTTCC 
ACGACGTGGG TCCCCTGCCA GATTTGTGAG ACAAGAGGAG CAGCAGCGGC ACCGCCCCTC 
ACCCTGGAGG GTCCTGTACA ATCTCATCAT GGGACTCCTG CCCTTACCCA GGGGCCACAG 
AGCCCCCGAG ATGGAGCCCA ATTAGGTGCC TGCACCCGCC CGGTGGACGT CAGGGACTCG 
GGGGGCAGGC CCCTCCCACC TCCTGACACC CTGGCCAGCG CGGGGGACTT TCTCTGCACC 
ATGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001120714.1
CDS66..371
Translation

Target ORF information:

RefSeq Version NM_001127242.3
Organism Homo sapiens(human)
Definition Homo sapiens BCL2 binding component 3 (BBC3), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001127242.3

ORF Insert Sequence:

1
61
121
181
241
301
ATGAAATTTG GCATGGGGTC TGCCCAGGCA TGTCCATGCC AGGTGCCCAG GGCTGCTTCC 
ACGACGTGGG TCCCCTGCCA GATTTGTGAG ACAAGAGGAG CAGCAGCGGC ACCGCCCCTC 
ACCCTGGAGG GTCCTGTACA ATCTCATCAT GGGACTCCTG CCCTTACCCA GGGGCCACAG 
AGCCCCCGAG ATGGAGCCCA ATTAGGTGCC TGCACCCGCC CGGTGGACGT CAGGGACTCG 
GGGGGCAGGC CCCTCCCACC TCCTGACACC CTGGCCAGCG CGGGGGACTT TCTCTGCACC 
ATGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19976
Clone ID Related Accession (Same CDS sequence) NM_001127240.3 , NM_001127240.2
Accession Version NM_001127240.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 786bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2020-01-04
Organism Homo sapiens(human)
Product bcl-2-binding component 3 isoform 1
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008532.7. On Jun 2, 2019 this sequence version replaced NM_001127240.2. Transcript Variant: This variant (1) includes an alternate exon in the 5' coding region, resulting in a frameshift for the remainder of the CDS, compared to variant 2. The encoded isoform (1, also known as PUMA-gamma) has the same N-terminus but is otherwise distinct and longer, compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF354654.1, ERR279827.7607.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02400288, SAMN02400289 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 11463392 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGAAATTTG GCATGGGGTC TGCCCAGGCA TGTCCATGCC AGGTGCCCAG GGCTGCTTCC 
ACGACGTGGG TCCCCTGCCA GATTTGTGGC CCCAGGGAGC GCCATGGCCC GCGCACGCCA 
GGAGGGCAGC TCCCCGGAGC CCGTAGAGGG CCTGGCCCGC GACGGCCCGC GCCCCTTCCC 
GCTCGGCCGC CTGGTGCCCT CGGCAGTGTC CTGCGGCCTC TGCGAGCCCG GCCTGGCTGC 
CGCCCCCGCC GCCCCCACCC TGCTGCCCGC TGCCTACCTC TGCGCCCCCA CCGCCCCACC 
CGCCGTCACC GCCGCCCTGG GGGGTTCCCG CTGGCCTGGG GGTCCCCGCA GCCGGCCCCG 
AGGCCCGCGC CCGGACGGTC CTCAGCCCTC GCTCTCGCTG GCGGAGCAGC ACCTGGAGTC 
GCCCGTGCCC AGCGCCCCGG GGGCTCTGGC GGGCGGTCCC ACCCAGGCGG CCCCGGGAGT 
CCGCGGGGAG GAGGAACAGT GGGCCCGGGA GATCGGGGCC CAGCTGCGGC GGATGGCGGA 
CGACCTCAAC GCACAGTACG AGCGGCGGAG ACAAGAGGAG CAGCAGCGGC ACCGCCCCTC 
ACCCTGGAGG GTCCTGTACA ATCTCATCAT GGGACTCCTG CCCTTACCCA GGGGCCACAG 
AGCCCCCGAG ATGGAGCCCA ATTAGGTGCC TGCACCCGCC CGGTGGACGT CAGGGACTCG 
GGGGGCAGGC CCCTCCCACC TCCTGACACC CTGGCCAGCG CGGGGGACTT TCTCTGCACC 
ATGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001120712.1
CDS66..851
Translation

Target ORF information:

RefSeq Version NM_001127240.3
Organism Homo sapiens(human)
Definition Homo sapiens BCL2 binding component 3 (BBC3), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001127240.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGAAATTTG GCATGGGGTC TGCCCAGGCA TGTCCATGCC AGGTGCCCAG GGCTGCTTCC 
ACGACGTGGG TCCCCTGCCA GATTTGTGGC CCCAGGGAGC GCCATGGCCC GCGCACGCCA 
GGAGGGCAGC TCCCCGGAGC CCGTAGAGGG CCTGGCCCGC GACGGCCCGC GCCCCTTCCC 
GCTCGGCCGC CTGGTGCCCT CGGCAGTGTC CTGCGGCCTC TGCGAGCCCG GCCTGGCTGC 
CGCCCCCGCC GCCCCCACCC TGCTGCCCGC TGCCTACCTC TGCGCCCCCA CCGCCCCACC 
CGCCGTCACC GCCGCCCTGG GGGGTTCCCG CTGGCCTGGG GGTCCCCGCA GCCGGCCCCG 
AGGCCCGCGC CCGGACGGTC CTCAGCCCTC GCTCTCGCTG GCGGAGCAGC ACCTGGAGTC 
GCCCGTGCCC AGCGCCCCGG GGGCTCTGGC GGGCGGTCCC ACCCAGGCGG CCCCGGGAGT 
CCGCGGGGAG GAGGAACAGT GGGCCCGGGA GATCGGGGCC CAGCTGCGGC GGATGGCGGA 
CGACCTCAAC GCACAGTACG AGCGGCGGAG ACAAGAGGAG CAGCAGCGGC ACCGCCCCTC 
ACCCTGGAGG GTCCTGTACA ATCTCATCAT GGGACTCCTG CCCTTACCCA GGGGCCACAG 
AGCCCCCGAG ATGGAGCCCA ATTAGGTGCC TGCACCCGCC CGGTGGACGT CAGGGACTCG 
GGGGGCAGGC CCCTCCCACC TCCTGACACC CTGGCCAGCG CGGGGGACTT TCTCTGCACC 
ATGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19976
Clone ID Related Accession (Same CDS sequence) NM_001127240.3 , NM_001127240.2
Accession Version NM_001127240.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 786bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-03-03
Organism Homo sapiens(human)
Product bcl-2-binding component 3 isoform 1
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF354654.1, BG258126.1, U82987.1 and AA887822.1. This sequence is a reference standard in the RefSeqGene project. On Dec 30, 2011 this sequence version replaced NM_001127240.1. Transcript Variant: This variant (1) includes an alternate exon in the 5' coding region, resulting in a frameshift for the remainder of the CDS, compared to variant 2. The encoded isoform (1, also known as PUMA-gamma) has the same N-terminus but is otherwise distinct and longer, compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF354654.1, ERR279827.7607.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02400288, SAMN02400289 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 11463392 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGAAATTTG GCATGGGGTC TGCCCAGGCA TGTCCATGCC AGGTGCCCAG GGCTGCTTCC 
ACGACGTGGG TCCCCTGCCA GATTTGTGGC CCCAGGGAGC GCCATGGCCC GCGCACGCCA 
GGAGGGCAGC TCCCCGGAGC CCGTAGAGGG CCTGGCCCGC GACGGCCCGC GCCCCTTCCC 
GCTCGGCCGC CTGGTGCCCT CGGCAGTGTC CTGCGGCCTC TGCGAGCCCG GCCTGGCTGC 
CGCCCCCGCC GCCCCCACCC TGCTGCCCGC TGCCTACCTC TGCGCCCCCA CCGCCCCACC 
CGCCGTCACC GCCGCCCTGG GGGGTTCCCG CTGGCCTGGG GGTCCCCGCA GCCGGCCCCG 
AGGCCCGCGC CCGGACGGTC CTCAGCCCTC GCTCTCGCTG GCGGAGCAGC ACCTGGAGTC 
GCCCGTGCCC AGCGCCCCGG GGGCTCTGGC GGGCGGTCCC ACCCAGGCGG CCCCGGGAGT 
CCGCGGGGAG GAGGAACAGT GGGCCCGGGA GATCGGGGCC CAGCTGCGGC GGATGGCGGA 
CGACCTCAAC GCACAGTACG AGCGGCGGAG ACAAGAGGAG CAGCAGCGGC ACCGCCCCTC 
ACCCTGGAGG GTCCTGTACA ATCTCATCAT GGGACTCCTG CCCTTACCCA GGGGCCACAG 
AGCCCCCGAG ATGGAGCCCA ATTAGGTGCC TGCACCCGCC CGGTGGACGT CAGGGACTCG 
GGGGGCAGGC CCCTCCCACC TCCTGACACC CTGGCCAGCG CGGGGGACTT TCTCTGCACC 
ATGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001120712.1
CDS165..950
Translation

Target ORF information:

RefSeq Version NM_001127240.2
Organism Homo sapiens(human)
Definition Homo sapiens BCL2 binding component 3 (BBC3), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001127240.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGAAATTTG GCATGGGGTC TGCCCAGGCA TGTCCATGCC AGGTGCCCAG GGCTGCTTCC 
ACGACGTGGG TCCCCTGCCA GATTTGTGGC CCCAGGGAGC GCCATGGCCC GCGCACGCCA 
GGAGGGCAGC TCCCCGGAGC CCGTAGAGGG CCTGGCCCGC GACGGCCCGC GCCCCTTCCC 
GCTCGGCCGC CTGGTGCCCT CGGCAGTGTC CTGCGGCCTC TGCGAGCCCG GCCTGGCTGC 
CGCCCCCGCC GCCCCCACCC TGCTGCCCGC TGCCTACCTC TGCGCCCCCA CCGCCCCACC 
CGCCGTCACC GCCGCCCTGG GGGGTTCCCG CTGGCCTGGG GGTCCCCGCA GCCGGCCCCG 
AGGCCCGCGC CCGGACGGTC CTCAGCCCTC GCTCTCGCTG GCGGAGCAGC ACCTGGAGTC 
GCCCGTGCCC AGCGCCCCGG GGGCTCTGGC GGGCGGTCCC ACCCAGGCGG CCCCGGGAGT 
CCGCGGGGAG GAGGAACAGT GGGCCCGGGA GATCGGGGCC CAGCTGCGGC GGATGGCGGA 
CGACCTCAAC GCACAGTACG AGCGGCGGAG ACAAGAGGAG CAGCAGCGGC ACCGCCCCTC 
ACCCTGGAGG GTCCTGTACA ATCTCATCAT GGGACTCCTG CCCTTACCCA GGGGCCACAG 
AGCCCCCGAG ATGGAGCCCA ATTAGGTGCC TGCACCCGCC CGGTGGACGT CAGGGACTCG 
GGGGGCAGGC CCCTCCCACC TCCTGACACC CTGGCCAGCG CGGGGGACTT TCTCTGCACC 
ATGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20134
Clone ID Related Accession (Same CDS sequence) NM_001127241.3 , NM_001127241.2
Accession Version NM_001127241.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 396bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2020-01-03
Organism Homo sapiens(human)
Product bcl-2-binding component 3 isoform 2
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008532.7. On Jun 2, 2019 this sequence version replaced NM_001127241.2. Transcript Variant: This variant (2) encodes isoform 2 (also known as PUMA-beta), which includes the C-terminal BH3 domain and can localize to the mitochondria. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF354655.1, SRR1163655.659098.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02400288, SAMN02400289 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 11463392 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
ATGAAATTTG GCATGGGGTC TGCCCAGGCA TGTCCATGCC AGGTGCCCAG GGCTGCTTCC 
ACGACGTGGG TCCCCTGCCA GATTTGTGGT CCTCAGCCCT CGCTCTCGCT GGCGGAGCAG 
CACCTGGAGT CGCCCGTGCC CAGCGCCCCG GGGGCTCTGG CGGGCGGTCC CACCCAGGCG 
GCCCCGGGAG TCCGCGGGGA GGAGGAACAG TGGGCCCGGG AGATCGGGGC CCAGCTGCGG 
CGGATGGCGG ACGACCTCAA CGCACAGTAC GAGCGGCGGA GACAAGAGGA GCAGCAGCGG 
CACCGCCCCT CACCCTGGAG GGTCCTGTAC AATCTCATCA TGGGACTCCT GCCCTTACCC 
AGGGGCCACA GAGCCCCCGA GATGGAGCCC AATTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001120713.1
CDS66..461
Translation

Target ORF information:

RefSeq Version NM_001127241.3
Organism Homo sapiens(human)
Definition Homo sapiens BCL2 binding component 3 (BBC3), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001127241.3

ORF Insert Sequence:

1
61
121
181
241
301
361
ATGAAATTTG GCATGGGGTC TGCCCAGGCA TGTCCATGCC AGGTGCCCAG GGCTGCTTCC 
ACGACGTGGG TCCCCTGCCA GATTTGTGGT CCTCAGCCCT CGCTCTCGCT GGCGGAGCAG 
CACCTGGAGT CGCCCGTGCC CAGCGCCCCG GGGGCTCTGG CGGGCGGTCC CACCCAGGCG 
GCCCCGGGAG TCCGCGGGGA GGAGGAACAG TGGGCCCGGG AGATCGGGGC CCAGCTGCGG 
CGGATGGCGG ACGACCTCAA CGCACAGTAC GAGCGGCGGA GACAAGAGGA GCAGCAGCGG 
CACCGCCCCT CACCCTGGAG GGTCCTGTAC AATCTCATCA TGGGACTCCT GCCCTTACCC 
AGGGGCCACA GAGCCCCCGA GATGGAGCCC AATTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20134
Clone ID Related Accession (Same CDS sequence) NM_001127241.3 , NM_001127241.2
Accession Version NM_001127241.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 396bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-03-03
Organism Homo sapiens(human)
Product bcl-2-binding component 3 isoform 2
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF354655.1, BG258126.1, U82987.1 and AA887822.1. On Dec 30, 2011 this sequence version replaced NM_001127241.1. Transcript Variant: This variant (2) encodes isoform 2 (also known as PUMA-beta), which includes the C-terminal BH3 domain and can localize to the mitochondria. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF354655.1, SRR1163655.659098.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02400288, SAMN02400289 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 11463392 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
ATGAAATTTG GCATGGGGTC TGCCCAGGCA TGTCCATGCC AGGTGCCCAG GGCTGCTTCC 
ACGACGTGGG TCCCCTGCCA GATTTGTGGT CCTCAGCCCT CGCTCTCGCT GGCGGAGCAG 
CACCTGGAGT CGCCCGTGCC CAGCGCCCCG GGGGCTCTGG CGGGCGGTCC CACCCAGGCG 
GCCCCGGGAG TCCGCGGGGA GGAGGAACAG TGGGCCCGGG AGATCGGGGC CCAGCTGCGG 
CGGATGGCGG ACGACCTCAA CGCACAGTAC GAGCGGCGGA GACAAGAGGA GCAGCAGCGG 
CACCGCCCCT CACCCTGGAG GGTCCTGTAC AATCTCATCA TGGGACTCCT GCCCTTACCC 
AGGGGCCACA GAGCCCCCGA GATGGAGCCC AATTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001120713.1
CDS165..560
Translation

Target ORF information:

RefSeq Version NM_001127241.2
Organism Homo sapiens(human)
Definition Homo sapiens BCL2 binding component 3 (BBC3), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001127241.2

ORF Insert Sequence:

1
61
121
181
241
301
361
ATGAAATTTG GCATGGGGTC TGCCCAGGCA TGTCCATGCC AGGTGCCCAG GGCTGCTTCC 
ACGACGTGGG TCCCCTGCCA GATTTGTGGT CCTCAGCCCT CGCTCTCGCT GGCGGAGCAG 
CACCTGGAGT CGCCCGTGCC CAGCGCCCCG GGGGCTCTGG CGGGCGGTCC CACCCAGGCG 
GCCCCGGGAG TCCGCGGGGA GGAGGAACAG TGGGCCCGGG AGATCGGGGC CCAGCTGCGG 
CGGATGGCGG ACGACCTCAA CGCACAGTAC GAGCGGCGGA GACAAGAGGA GCAGCAGCGG 
CACCGCCCCT CACCCTGGAG GGTCCTGTAC AATCTCATCA TGGGACTCCT GCCCTTACCC 
AGGGGCCACA GAGCCCCCGA GATGGAGCCC AATTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu67065
Clone ID Related Accession (Same CDS sequence) XM_011526722.2
Accession Version XM_011526722.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 630bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product bcl-2-binding component 3 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011526722.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
ATGTTGGGGT TTCTGACCCG TCGGGGTGTC TGCGCCCCAG GGAGCGCCAT GGCCCGCGCA 
CGCCAGGAGG GCAGCTCCCC GGAGCCCGTA GAGGGCCTGG CCCGCGACGG CCCGCGCCCC 
TTCCCGCTCG GCCGCCTGGT GCCCTCGGCA GTGTCCTGCG GCCTCTGCGA GCCCGGCCTG 
GCTGCCGCCC CCGCCGCCCC CACCCTGCTG CCCGCTGCCT ACCTCTGCGC CCCCACCGCC 
CCACCCGCCG TCACCGCCGC CCTGGGGGGT TCCCGCTGGC CTGGGGGTCC CCGCAGCCGG 
CCCCGAGGCC CGCGCCCGGA CGGTCCTCAG CCCTCGCTCT CGCTGGCGGA GCAGCACCTG 
GAGTCGCCCG TGCCCAGCGC CCCGGGGGCT CTGGCGGGCG GTCCCACCCA GGCGGCCCCG 
GGAGTCCGCG GGGAGGAGGA ACAGTGGGCC CGGGAGATCG GGGCCCAGCT GCGGCGGATG 
GCGGACGACC TCAACGCACA GTACGAGCGG CGGAGACAAG AGGAGCAGCA GCGGCACCGC 
CCCTCACCCT GGAGGGTCCT GTACAATCTC ATCATGGGAC TCCTGCCCTT ACCCAGGGGC 
CACAGAGCCC CCGAGATGGA GCCCAATTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011525024.1
CDS126..755
Translation

Target ORF information:

RefSeq Version XM_011526722.2
Organism Homo sapiens(human)
Definition Homo sapiens BCL2 binding component 3 (BBC3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011526722.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGTTGGGGT TTCTGACCCG TCGGGGTGTC TGCGCCCCAG GGAGCGCCAT GGCCCGCGCA 
CGCCAGGAGG GCAGCTCCCC GGAGCCCGTA GAGGGCCTGG CCCGCGACGG CCCGCGCCCC 
TTCCCGCTCG GCCGCCTGGT GCCCTCGGCA GTGTCCTGCG GCCTCTGCGA GCCCGGCCTG 
GCTGCCGCCC CCGCCGCCCC CACCCTGCTG CCCGCTGCCT ACCTCTGCGC CCCCACCGCC 
CCACCCGCCG TCACCGCCGC CCTGGGGGGT TCCCGCTGGC CTGGGGGTCC CCGCAGCCGG 
CCCCGAGGCC CGCGCCCGGA CGGTCCTCAG CCCTCGCTCT CGCTGGCGGA GCAGCACCTG 
GAGTCGCCCG TGCCCAGCGC CCCGGGGGCT CTGGCGGGCG GTCCCACCCA GGCGGCCCCG 
GGAGTCCGCG GGGAGGAGGA ACAGTGGGCC CGGGAGATCG GGGCCCAGCT GCGGCGGATG 
GCGGACGACC TCAACGCACA GTACGAGCGG CGGAGACAAG AGGAGCAGCA GCGGCACCGC 
CCCTCACCCT GGAGGGTCCT GTACAATCTC ATCATGGGAC TCCTGCCCTT ACCCAGGGGC 
CACAGAGCCC CCGAGATGGA GCCCAATTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed