PGK1 cDNA ORF clone, Homo sapiens(human)
| Gene Symbol | PGK1 |
| Entrez Gene ID | 5230 |
| Full Name | phosphoglycerate kinase 1 |
| Synonyms | HEL-S-68p,MIG10,PGKA |
| General protein information |
|
| Gene Type | protein-coding |
| Organism | Homo sapiens(human) |
| Genome | |
| Summary | The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome. [provided by RefSeq, Jan 2014]. |
| Disorder MIM: | |
| Disorder Html: | Phosphoglycerate kinase 1 deficiency, 300653 (3) |
mRNA and Protein(s)
| mRNA | Protein | Name |
|---|---|---|
| NM_000291.3 | NP_000282.1 | phosphoglycerate kinase 1 |
| NM_000291.4 | NP_000282.1 | phosphoglycerate kinase 1 |
| Canis lupus familiaris (dog) | PGK1 | XP_849992.1 |
| Danio rerio (zebrafish) | pgk1 | NP_998552.1 |
| Schizosaccharomyces pombe (fission yeast) | pgk1 | NP_596730.1 |
| PGK1 | XP_001100787.1 | |
| Drosophila melanogaster (fruit fly) | Pgk | NP_001259958.1 |
| Anopheles gambiae (African malaria mosquito) | AgaP_AGAP008802 | XP_314928.2 |
| Arabidopsis thaliana (thale cress) | PGK1 | NP_187884.1 |
| Mus musculus (house mouse) | Pgk1 | NP_032854.2 |
| Rattus norvegicus (Norway rat) | Pgk1 | NP_445743.2 |
| Gallus gallus (chicken) | PGK1 | NP_990316.1 |
| Saccharomyces cerevisiae (baker's yeast) | PGK1 | NP_009938.2 |
| Arabidopsis thaliana (thale cress) | AT1G56190 | NP_176015.1 |
| Pan troglodytes (chimpanzee) | PGK1 | NP_001129210.1 |
| Caenorhabditis elegans (roundworm) | pgk-1 | NP_491245.1 |
| Kluyveromyces lactis | KLLA0A11011g | XP_451479.1 |
| Eremothecium gossypii | AGOS_AEL038C | NP_984823.2 |
| Magnaporthe oryzae (rice blast fungus) | MGG_05063 | XP_003712576.1 |
| Neurospora crassa | NCU07914 | XP_962430.1 |
| Homo sapiens (human) | PGK1 | NP_000282.1 |
| Bos taurus (cattle) | PGK1 | NP_001029471.1 |
| Xenopus tropicalis (tropical clawed frog) | pgk1 | NP_001016545.1 |
Related articles in PubMed
MetaLnc9 Facilitates Lung Cancer Metastasis via a PGK1-Activated AKT/mTOR Pathway.
Yu T, Zhao Y, Hu Z, Li J, Chu D, Zhang J, Li Z, Chen B, Zhang X, Pan H, Li S, Lin H, Liu L, Yan M, He X, Yao M
Cancer research77(21)5782-5794(2017 Nov)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
Data show that LINC00963 (MetaLnc9) interacted with the glycolytic kinase PGK1 and prevented its ubiquitination in non-small cell lung cancer (NSCLC) cells, leading to activation of the oncogenic AKT/mTOR signaling pathway.
Title: MetaLnc9 Facilitates Lung Cancer Metastasis via a PGK1-Activated AKT/mTOR Pathway.
Data suggest that, in breast cancer cells, MYC acts as an upstream regulator leading to PGK1 activation. (MYC, proto-oncogene c-myc; PGK1, phosphoglycerate kinase 1)
Title: 17beta-hydroxysteroid dehydrogenase type 5 is negatively correlated to apoptosis inhibitor GRP78 and tumor-secreted protein PGK1, and modulates breast cancer cell viability and proliferation.
Acetylated PGK1 binds to and phosphorylates Beclin1 at S30, leading to activation of the VPS34-Beclin1 complex to initiate autophagosomal formation.
Title: Phosphoglycerate Kinase 1 Phosphorylates Beclin1 to Induce Autophagy.
PGK1, a glycolytic enzyme catalyzing the conversion of 3-phosphoglycerate into 2-phosphoglycerate, has increased expression in synovial tissues and blood of rheumatoid arthritis, which may be involved in pro-inflammation and synovial hyperplasia of the disease
Title: PGK1, a glucose metabolism enzyme, may play an important role in rheumatoid arthritis.
Mitochindrial PGK1 acts as a protein kinase in coordinating glycolysis and the tricarboxylic acid cycle, which is instrumental in cancer metabolism and tumorigenesis.
Title: Mitochondria-Translocated PGK1 Functions as a Protein Kinase to Coordinate Glycolysis and the TCA Cycle in Tumorigenesis.
The following PGK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PGK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OHu19088 |
|
| Clone ID Related Accession (Same CDS sequence) | NM_000291.3 , NM_000291.4 | |
| Accession Version | NM_000291.3 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1254bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-12-05 | |
| Organism | Homo sapiens(human) | |
| Product | phosphoglycerate kinase 1 | |
| Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC336483.1, BC103752.1 and BU677171.1. This sequence is a reference standard in the RefSeqGene project. On Apr 18, 2008 this sequence version replaced NM_000291.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB062432.1, SRR1803617.126569.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## multifunctional gene product(s) :: PMID: 2324090, 11130727 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGTCGCTTT CTAACAAGCT GACGCTGGAC AAGCTGGACG TTAAAGGGAA GCGGGTCGTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_000282.1 |
| CDS | 173..1426 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_000291.3 |
ORF Insert Sequence:
1 | ATGTCGCTTT CTAACAAGCT GACGCTGGAC AAGCTGGACG TTAAAGGGAA GCGGGTCGTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu19088 |
|
| Clone ID Related Accession (Same CDS sequence) | NM_000291.3 , NM_000291.4 | |
| Accession Version | NM_000291.4 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1254bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2020-01-01 | |
| Organism | Homo sapiens(human) | |
| Product | phosphoglycerate kinase 1 | |
| Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC336483.1, BC103752.1, BU677171.1 and AL049589.15. On Dec 7, 2018 this sequence version replaced NM_000291.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.268563.1, SRR1803616.55464.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000373316.5/ ENSP00000362413.4 multifunctional gene product(s) :: PMID: 2324090, 11130727 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length. | |
1 | ATGTCGCTTT CTAACAAGCT GACGCTGGAC AAGCTGGACG TTAAAGGGAA GCGGGTCGTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_000282.1 |
| CDS | 94..1347 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_000291.4 |
ORF Insert Sequence:
1 | ATGTCGCTTT CTAACAAGCT GACGCTGGAC AAGCTGGACG TTAAAGGGAA GCGGGTCGTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
 |
MetaLnc9 Facilitates Lung Cancer Metastasis via a PGK1-Activated AKT/mTOR Pathway. |