Diaph2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Gene Symbol	Diaph2
Entrez Gene ID	54004
Full Name	diaphanous related formin 2
Synonyms	Dia3,Diap2,Drf2,E430022I22Rik
General protein information	Preferred Names diaphanous related formin 2 Names protein diaphanous homolog 2 diaphanous homolog 2 mDia3 isoform
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X\|X E3

mRNA and Protein(s)

mRNA	Protein	Name
XM_030251385.1	XP_030107245.1	protein diaphanous homolog 2 isoform X3
XM_006528581.3	XP_006528644.1	protein diaphanous homolog 2 isoform X1
XM_006528581.4	XP_006528644.1	protein diaphanous homolog 2 isoform X1
XM_006528582.4	XP_006528645.1	protein diaphanous homolog 2 isoform X2
NM_172493.2	NP_766081.1	protein diaphanous homolog 2
XM_006528582.3	XP_006528645.1	protein diaphanous homolog 2 isoform X2

Pathways from BioSystems
Homologies

Mus musculus (house mouse) Diap2 NP_766081.1

Rattus norvegicus (Norway rat) Diaph2 XP_228441.7
Gene Ontology
Bibliography

Related articles in PubMed

mDia and ROCK Mediate Actin-Dependent Presynaptic Remodeling Regulating Synaptic Efficacy and Anxiety.
Deguchi Y, Harada M, Shinohara R, Lazarus M, Cherasse Y, Urade Y, Yamada D, Sekiguchi M, Watanabe D, Furuyashiki T, Narumiya S
Cell reports17(9)2405-2417(2016 11)

Expression of multiple formins in adult tissues and during developmental stages of mouse brain.
Dutta P, Maiti S
Gene expression patterns : GEP19(1-2)52-9(2015 Sep-Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

mDia induces circumferential actin filaments around the edge of the synaptic cleft, which contract the presynaptic terminals in a ROCK-dependent manner.
Title: mDia and ROCK Mediate Actin-Dependent Presynaptic Remodeling Regulating Synaptic Efficacy and Anxiety.

study examined how mDia functions in axon guidance in vivo and in vitro; findings show mDia plays multiple roles in the proper formation of the neural network
Title: EphA4-dependent axon retraction and midline localization of Ephrin-B3 are disrupted in the spinal cord of mice lacking mDia1 and mDia3 in combination.

These results imply that RhoD activated by FGF signaling forms cytoneme-like protrusions through activation of mDia3C, which induces actin filament formation
Title: RhoD activated by fibroblast growth factor induces cytoneme-like cellular protrusions through mDia3C.

actin cytoskeleton regulated by Rho-mDia pathway is critical for the integrity of the adherens junctions and the polarity of neuroepithelial cells
Title: Deficiency of mDia, an actin nucleator, disrupts integrity of neuroepithelium and causes periventricular dysplasia.

analysis of interactions between mDia isoforms 1, 2, and 3 and RhoA, Rac1, and Cdc42
Title: Specificity of interactions between mDia isoforms and Rho proteins.

The following Diaph2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Diaph2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu90415	XM_030251385.1 Latest version!	Mus musculus diaphanous related formin 2 (Diaph2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu17576	NM_172493.2 Latest version!	Mus musculus diaphanous related formin 2 (Diaph2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OMu56538	XM_006528581.4 Latest version!	Mus musculus diaphanous related formin 2 (Diaph2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu56539	XM_006528582.4 Latest version!	Mus musculus diaphanous related formin 2 (Diaph2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OMu56538	XM_006528581.3	Mus musculus diaphanous related formin 2 (Diaph2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OMu56539	XM_006528582.3	Mus musculus diaphanous related formin 2 (Diaph2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Diaph2 ( XM_030251385.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107245.1 Mus musculus diaphanous related formin 2 (Diaph2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu90415
Clone ID Related Accession (Same CDS sequence)	XM_030251385.1
Accession Version	XM_030251385.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein diaphanous homolog 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TCGGGGCGGC GGCGTCGGGA GCCGGAGGCG GCGGCGGTGG CGGCGAGGAA 
CACGGCGGGG GCCGGAGCAA CAAGCGAGGC GCGGGGAACC GGGCCGCCAA CGAAGAGGAG 
ACGAGAAACA AACCCAAACT GAACATTCAA ATAAAAACTT TGGCAGATGA TGTGCGTGAC 
CGAATTACAA GTTTCAGAAA ATCTGCCACC AAGAGAGAAA AGCCTGTTAT TCAACATTCT 
ATTGATTATC AAACTGCAGT GGTTGAGATT CCCCCAGCTC TGATAGTACA TGATGACCGA 
TCTTTGATTT TGTCTGAAAA GGAAGTTCTG GACCTCTTTG AGAAAATGAT GGAAGATATG 
AACCTTAATG AAGAAAAGAA AGCTCCTTTG CGAAAGAAGG ATTTTAGCAT CAAGCGTGAG 
ATGGTTGTCC AGTATATTTC TGCTACTTCC AAATCTATAG TTGGAAGTAA AGTTCTGGGT 
GGCCTGAAAA ACAGCAAACA TGAGTTCACC CTTTCTTCAC AAGAATATGT TCATGAATTA 
CGATCTGGCA TCTCAGATGA GAAACTTCTC AACTGCCTGG AATCTTTGAG AGTGTCTTTA 
ACCAGCCATC CTGTCAGCTG GGTCAACAAC TTTGGCTATG AAGGACTTGG AGTCTTACTG 
GATGTGCTGG AGAAGCTTCT GGACAAAAAA CAGCAAGAAA ATATCGACAA GAAGAATCAG 
TACAAAGTCA TTCAGTGCCT GAAAGCATTT ATGAATAATA AGTTTGGACT ACAAAGAATT 
CTAGGAGACG AGAGAAGCCT TTTACTGTTG GCAAGAGCAA TTGACCCGAA ACAACAGAAT 
ATGATGACTG AAATAGTAAA AATCCTTTCT GCTATTTGCA TTGTTGGGGA AGAAAACATT 
CTAGACAAAC TTTTAGGGGG CATCACTGCT GCAGCTGAAC TAAATAATAG AGAACGATTT 
TCACCAATTG TGGAAGGCTT AGAAAATAAT GAAGCCTTGC ATCTCCAGGT GGCCTGCATG 
CAGTTCATAA ATGCACTTGT CACCTCTCCA TATGATCTTG ATTTTCGAAT TCATTTGAGG 
AATGAATTCC TTCGTTGTGG ATTAAAAGCA ATGCTCCCTA CTCTTAAAGA GATAGAGAAT 
GAAGGGCTTG ATATTCAGTT GAGAGTGTTT GAAGAGAACA AAGAAGATGA CCTAAGTGAA 
TTATCCCACC GTCTCAATGA CATTCGAGCT GAAATGGATG ATATTAATGA AGTCTACCAT 
CTGCTGTATA ATATGCTGAA GGACACTGCT GCGGAACCTT ACTTATTATC CATTCTCCAA 
CACTTTCTGC TCATCAGAAA TGATTACTAC ATCAGGCCAC AGTATTATAA AATCATTGAG 
GAGTGTGTTT CACAGATAGT GCTACACTGC AGTGGCATGG ACCCAGACTT CAAGTACCGG 
CAGCGCATAG ACTTTGATTT CACTCATCTC CTAGATGCTT GTGTAAATAA GGCAAAAGTT 
GAAGAAAATG AGCAAAAAGC AATGGAATTT TCAAAGAAGT TCGATGAAGA ATTCACAGCT 
CGTCAGGAAG CTCAAGCAGA GCTTCAGAAA AGAGATGAAA AAATCAAAGA ACTGGAAACA 
GAAATCCAGC AGCTTCGAGG ACAGGGAGTT CCATCTGCAA TTCCAGGTCC ACCTCCACCA 
CCTCCACTGC CAGGTGCAGG ACCATGCCCA CCACCACCAC CTCCTCCTCC TCCACCACCT 
CCACTTCCAG GAGTCGTCCC TCCTCCTCCT CCACCATTAC CTGGGATGCC TGGTATTCCA 
CCACCACCTC CACCACCTTT GTCTGGAGTA CCTCCTCCAC CACCACCCCC TGGGGGAGTT 
TTTCCTCTCC TATCAGGACC AATTGAGCTA CCATATGGAA TGAAGCAGAA AAAATTATAT 
AAGCCTGACA TACCTATGAA AAGAATCAAC TGGTCCAAGA TTGAGCCTAA AGAATTATCC 
GAAAACTGTG TTTGGCTAAA ACTTAAAGAA GAGAAGTATG AGAATGCAGA TCTATTTGCA 
AAACTGGCCT TGACATTTCC TTCCCAGATG AAAGATTACA TGGCTGTAGC CTCCGAACTG 
AAGAAAATTA CTGCTGAAAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107245.1
CDS	307..2430
Translation	MEELGAAASGAGGGGGGGEEHGGGRSNKRGAGNRAANEEETRNKPKLNIQIKTLADDVRDRITSFRKSATKREKPVIQHSIDYQTAVVEIPPALIVHDDRSLILSEKEVLDLFEKMMEDMNLNEEKKAPLRKKDFSIKREMVVQYISATSKSIVGSKVLGGLKNSKHEFTLSSQEYVHELRSGISDEKLLNCLESLRVSLTSHPVSWVNNFGYEGLGVLLDVLEKLLDKKQQENIDKKNQYKVIQCLKAFMNNKFGLQRILGDERSLLLLARAIDPKQQNMMTEIVKILSAICIVGEENILDKLLGGITAAAELNNRERFSPIVEGLENNEALHLQVACMQFINALVTSPYDLDFRIHLRNEFLRCGLKAMLPTLKEIENEGLDIQLRVFEENKEDDLSELSHRLNDIRAEMDDINEVYHLLYNMLKDTAAEPYLLSILQHFLLIRNDYYIRPQYYKIIEECVSQIVLHCSGMDPDFKYRQRIDFDFTHLLDACVNKAKVEENEQKAMEFSKKFDEEFTARQEAQAELQKRDEKIKELETEIQQLRGQGVPSAIPGPPPPPPLPGAGPCPPPPPPPPPPPPLPGVVPPPPPPLPGMPGIPPPPPPPLSGVPPPPPPPGGVFPLLSGPIELPYGMKQKKLYKPDIPMKRINWSKIEPKELSENCVWLKLKEEKYENADLFAKLALTFPSQMKDYMAVASELKKITAER

Target ORF information:

RefSeq Version	XM_030251385.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus diaphanous related formin 2 (Diaph2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030251385.1

ORF Insert Sequence:

ATGGAGGAGC TCGGGGCGGC GGCGTCGGGA GCCGGAGGCG GCGGCGGTGG CGGCGAGGAA 
CACGGCGGGG GCCGGAGCAA CAAGCGAGGC GCGGGGAACC GGGCCGCCAA CGAAGAGGAG 
ACGAGAAACA AACCCAAACT GAACATTCAA ATAAAAACTT TGGCAGATGA TGTGCGTGAC 
CGAATTACAA GTTTCAGAAA ATCTGCCACC AAGAGAGAAA AGCCTGTTAT TCAACATTCT 
ATTGATTATC AAACTGCAGT GGTTGAGATT CCCCCAGCTC TGATAGTACA TGATGACCGA 
TCTTTGATTT TGTCTGAAAA GGAAGTTCTG GACCTCTTTG AGAAAATGAT GGAAGATATG 
AACCTTAATG AAGAAAAGAA AGCTCCTTTG CGAAAGAAGG ATTTTAGCAT CAAGCGTGAG 
ATGGTTGTCC AGTATATTTC TGCTACTTCC AAATCTATAG TTGGAAGTAA AGTTCTGGGT 
GGCCTGAAAA ACAGCAAACA TGAGTTCACC CTTTCTTCAC AAGAATATGT TCATGAATTA 
CGATCTGGCA TCTCAGATGA GAAACTTCTC AACTGCCTGG AATCTTTGAG AGTGTCTTTA 
ACCAGCCATC CTGTCAGCTG GGTCAACAAC TTTGGCTATG AAGGACTTGG AGTCTTACTG 
GATGTGCTGG AGAAGCTTCT GGACAAAAAA CAGCAAGAAA ATATCGACAA GAAGAATCAG 
TACAAAGTCA TTCAGTGCCT GAAAGCATTT ATGAATAATA AGTTTGGACT ACAAAGAATT 
CTAGGAGACG AGAGAAGCCT TTTACTGTTG GCAAGAGCAA TTGACCCGAA ACAACAGAAT 
ATGATGACTG AAATAGTAAA AATCCTTTCT GCTATTTGCA TTGTTGGGGA AGAAAACATT 
CTAGACAAAC TTTTAGGGGG CATCACTGCT GCAGCTGAAC TAAATAATAG AGAACGATTT 
TCACCAATTG TGGAAGGCTT AGAAAATAAT GAAGCCTTGC ATCTCCAGGT GGCCTGCATG 
CAGTTCATAA ATGCACTTGT CACCTCTCCA TATGATCTTG ATTTTCGAAT TCATTTGAGG 
AATGAATTCC TTCGTTGTGG ATTAAAAGCA ATGCTCCCTA CTCTTAAAGA GATAGAGAAT 
GAAGGGCTTG ATATTCAGTT GAGAGTGTTT GAAGAGAACA AAGAAGATGA CCTAAGTGAA 
TTATCCCACC GTCTCAATGA CATTCGAGCT GAAATGGATG ATATTAATGA AGTCTACCAT 
CTGCTGTATA ATATGCTGAA GGACACTGCT GCGGAACCTT ACTTATTATC CATTCTCCAA 
CACTTTCTGC TCATCAGAAA TGATTACTAC ATCAGGCCAC AGTATTATAA AATCATTGAG 
GAGTGTGTTT CACAGATAGT GCTACACTGC AGTGGCATGG ACCCAGACTT CAAGTACCGG 
CAGCGCATAG ACTTTGATTT CACTCATCTC CTAGATGCTT GTGTAAATAA GGCAAAAGTT 
GAAGAAAATG AGCAAAAAGC AATGGAATTT TCAAAGAAGT TCGATGAAGA ATTCACAGCT 
CGTCAGGAAG CTCAAGCAGA GCTTCAGAAA AGAGATGAAA AAATCAAAGA ACTGGAAACA 
GAAATCCAGC AGCTTCGAGG ACAGGGAGTT CCATCTGCAA TTCCAGGTCC ACCTCCACCA 
CCTCCACTGC CAGGTGCAGG ACCATGCCCA CCACCACCAC CTCCTCCTCC TCCACCACCT 
CCACTTCCAG GAGTCGTCCC TCCTCCTCCT CCACCATTAC CTGGGATGCC TGGTATTCCA 
CCACCACCTC CACCACCTTT GTCTGGAGTA CCTCCTCCAC CACCACCCCC TGGGGGAGTT 
TTTCCTCTCC TATCAGGACC AATTGAGCTA CCATATGGAA TGAAGCAGAA AAAATTATAT 
AAGCCTGACA TACCTATGAA AAGAATCAAC TGGTCCAAGA TTGAGCCTAA AGAATTATCC 
GAAAACTGTG TTTGGCTAAA ACTTAAAGAA GAGAAGTATG AGAATGCAGA TCTATTTGCA 
AAACTGGCCT TGACATTTCC TTCCCAGATG AAAGATTACA TGGCTGTAGC CTCCGAACTG 
AAGAAAATTA CTGCTGAAAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Diaph2 ( NM_172493.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766081.1 Mus musculus diaphanous related formin 2 (Diaph2), mRNA.

Price & Availability↑

CloneID	OMu17576
Clone ID Related Accession (Same CDS sequence)	NM_172493.2
Accession Version	NM_172493.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	protein diaphanous homolog 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL683856.10, AY312280.1, AL807775.8 and BQ030953.1. On Aug 28, 2010 this sequence version replaced NM_172493.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY312280.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGAGC TCGGGGCGGC GGCGTCGGGA GCCGGAGGCG GCGGCGGTGG CGGCGAGGAA 
CACGGCGGGG GCCGGAGCAA CAAGCGAGGC GCGGGGAACC GGGCCGCCAA CGAAGAGGAG 
ACGAGAAACA AACCCAAACT GAACATTCAA ATAAAAACTT TGGCAGATGA TGTGCGTGAC 
CGAATTACAA GTTTCAGAAA ATCTGCCACC AAGAGAGAAA AGCCTGTTAT TCAACATTCT 
ATTGATTATC AAACTGCAGT GGTTGAGATT CCCCCAGCTC TGATAGTACA TGATGACCGA 
TCTTTGATTT TGTCTGAAAA GGAAGTTCTG GACCTCTTTG AGAAAATGAT GGAAGATATG 
AACCTTAATG AAGAAAAGAA AGCTCCTTTG CGAAAGAAGG ATTTTAGCAT CAAGCGTGAG 
ATGGTTGTCC AGTATATTTC TGCTACTTCC AAATCTGGTG GCCTGAAAAA CAGCAAACAT 
GAGTTCACCC TTTCTTCACA AGAATATGTT CATGAATTAC GATCTGGCAT CTCAGATGAG 
AAACTTCTCA ACTGCCTGGA ATCTTTGAGA GTGTCTTTAA CCAGCCATCC TGTCAGCTGG 
GTCAACAACT TTGGCTATGA AGGACTTGGA GTCTTACTGG ATGTGCTGGA GAAGCTTCTG 
GACAAAAAAC AGCAAGAAAA TATCGACAAG AAGAATCAGT ACAAAGTCAT TCAGTGCCTG 
AAAGCATTTA TGAATAATAA GTTTGGACTA CAAAGAATTC TAGGAGACGA GAGAAGCCTT 
TTACTGTTGG CAAGAGCAAT TGACCCGAAA CAACAGAATA TGATGACTGA AATAGTAAAA 
ATCCTTTCTG CTATTTGCAT TGTTGGGGAA GAAAACATTC TAGACAAACT TTTAGGGGGC 
ATCACTGCTG CAGCTGAACT AAATAATAGA GAACGATTTT CACCAATTGT GGAAGGCTTA 
GAAAATAATG AAGCCTTGCA TCTCCAGGTG GCCTGCATGC AGTTCATAAA TGCACTTGTC 
ACCTCTCCAT ATGATCTTGA TTTTCGAATT CATTTGAGGA ATGAATTCCT TCGTTGTGGA 
TTAAAAGCAA TGCTCCCTAC TCTTAAAGAG ATAGAGAATG AAGGGCTTGA TATTCAGTTG 
AGAGTGTTTG AAGAGAACAA AGAAGATGAC CTAAGTGAAT TATCCCACCG TCTCAATGAC 
ATTCGAGCTG AAATGGATGA TATTAATGAA GTCTACCATC TGCTGTATAA TATGCTGAAG 
GACACTGCTG CGGAACCTTA CTTATTATCC ATTCTCCAAC ACTTTCTGCT CATCAGAAAT 
GATTACTACA TCAGGCCACA GTATTATAAA ATCATTGAGG AGTGTGTTTC ACAGATAGTG 
CTACACTGCA GTGGCATGGA CCCAGACTTC AAGTACCGGC AGCGCATAGA CTTTGATTTC 
ACTCATCTCC TAGATGCTTG TGTAAATAAG GCAAAAGTTG AAGAAAATGA GCAAAAAGCA 
ATGGAATTTT CAAAGAAGTT CGATGAAGAA TTCACAGCTC GTCAGGAAGC TCAAGCAGAG 
CTTCAGAAAA GAGATGAAAA AATCAAAGAA CTGGAAACAG AAATCCAGCA GCTTCGAGGA 
CAGGGAGTTC CATCTGCAAT TCCAGGTCCA CCTCCACCAC CTCCACTGCC AGGTGCAGGA 
CCATGCCCAC CACCACCACC TCCTCCTCCT CCACCACCTC CACTTCCAGG AGTCGTCCCT 
CCTCCTCCTC CACCATTACC TGGGATGCCT GGTATTCCAC CACCACCTCC ACCACCTTTG 
TCTGGAGTAC CTCCTCCACC ACCACCCCCT GGGGGAGTTT TTCCTCTCCT ATCAGGACCA 
ATTGAGCTAC CATATGGAAT GAAGCAGAAA AAATTATATA AGCCTGACAT ACCTATGAAA 
AGAATCAACT GGTCCAAGAT TGAGCCTAAA GAATTATCCG AAAACTGTGT TTGGCTAAAA 
CTTAAAGAAG AGAAGTATGA GAATGCAGAT CTATTTGCAA AACTGGCCTT GACATTTCCT 
TCCCAGATGA AAGGTCAAAG GAATACAGAG GCTGCAGAAG AAAATAGGAG TGGGCCTCCA 
AAGAAGAAAG TGAAGGAATT GAGAATTTTG GATACCAAAA CAGCCCAGAA TCTATCTATC 
TTCCTTGGAT CTTACCGCAT GCCATACGAG GAAATAAAGA ACATTATTTT GGAGGTTAAT 
GAAGAGATGC TCAGTGAGGC CCTAATCCAG AATCTTGTAA AATATCTTCC TGACCAGAAC 
GCACTCCGAG AACTAGCCCA GCTTAAGAGT GAATATGATG ATCTCTGTGA GCCTGAGCAG 
TTTGGTGTTG TGATGAGCAC GGTGAAAATG TTACGTCCTC GTCTCACGAG CATCCTTTTC 
AAACTCACCT TTGAAGAACA TGTCAACAAC ATCAAACCAA GCATCATAGC AGTAACTCTT 
GCATGTGAAG AACTGAAGAA AAGTGAAAGC TTTAAGAGAC TCTTAGAGTT GATTCTTCTG 
GTTGGAAACT ACATGAACTC AGGCTCCAGG AATGCCCAGT CTCTAGGTTT TAAGATTAAC 
TTTCTGTGTA AGATCAAAGA CACAAAATCA GCAGATCAAA AATCCACCCT TTTGCATTTT 
CTTGCTGAAA TCTGTGATGA GAAATACCGG GATATCCTGA AATTTCCTGA TGAGCTGGAG 
CATGTGGAAA GCGCAGGCAA AGTTTCAGCT CAAATACTCA AGAGCAACCT TGTAGCAATG 
GAACAAAGCA TTCTTCATCT GGAGAAAAAC ATCAAGAATT TCCCACCAGC AGAATCGCAC 
CATGATAAGT TTGTGGAAAA GATGATGAGC TTTACCCAGA ATGCCAGAGA ACAGTATGAC 
AAACTGTCCA CCATGCACAG CAATATGTTG AAGCTCTATG AGAGTCTGGG AGAATACTTC 
ATTTTTGACC CGAACACAGT AAACATGGAA GAATTTTTTG GTGATCTCAA CACCTTCAGA 
ACCCTATTTT TGGAAGCACT GAAAGAAAAC CACAAGAGAA AGGAAATGGA AGAGAAGAGC 
AGGAGAGCAA AGCTTGCAAA AGAGAAAGCG GAACAAGAGA AGCTGGAACG CCAGAAGAAG 
AAGAAGCAAC TCATTGACAT AAACAAAGAG GGTGATGAGA CGGGAGTGAT GGATAATCTT 
CTGGAAGCCC TACAGTCAGG TGCAGCATTC AGAGACCGCA GAAAGCGAAT TCCAAGAAAT 
CCAGATAACA GACGGCCCCC TCTGGAAAGA TCTCGCTCTC GCCACAATGG AGCCATGTCA 
TCCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766081.1
CDS	235..3543
Translation	MEELGAAASGAGGGGGGGEEHGGGRSNKRGAGNRAANEEETRNKPKLNIQIKTLADDVRDRITSFRKSATKREKPVIQHSIDYQTAVVEIPPALIVHDDRSLILSEKEVLDLFEKMMEDMNLNEEKKAPLRKKDFSIKREMVVQYISATSKSGGLKNSKHEFTLSSQEYVHELRSGISDEKLLNCLESLRVSLTSHPVSWVNNFGYEGLGVLLDVLEKLLDKKQQENIDKKNQYKVIQCLKAFMNNKFGLQRILGDERSLLLLARAIDPKQQNMMTEIVKILSAICIVGEENILDKLLGGITAAAELNNRERFSPIVEGLENNEALHLQVACMQFINALVTSPYDLDFRIHLRNEFLRCGLKAMLPTLKEIENEGLDIQLRVFEENKEDDLSELSHRLNDIRAEMDDINEVYHLLYNMLKDTAAEPYLLSILQHFLLIRNDYYIRPQYYKIIEECVSQIVLHCSGMDPDFKYRQRIDFDFTHLLDACVNKAKVEENEQKAMEFSKKFDEEFTARQEAQAELQKRDEKIKELETEIQQLRGQGVPSAIPGPPPPPPLPGAGPCPPPPPPPPPPPPLPGVVPPPPPPLPGMPGIPPPPPPPLSGVPPPPPPPGGVFPLLSGPIELPYGMKQKKLYKPDIPMKRINWSKIEPKELSENCVWLKLKEEKYENADLFAKLALTFPSQMKGQRNTEAAEENRSGPPKKKVKELRILDTKTAQNLSIFLGSYRMPYEEIKNIILEVNEEMLSEALIQNLVKYLPDQNALRELAQLKSEYDDLCEPEQFGVVMSTVKMLRPRLTSILFKLTFEEHVNNIKPSIIAVTLACEELKKSESFKRLLELILLVGNYMNSGSRNAQSLGFKINFLCKIKDTKSADQKSTLLHFLAEICDEKYRDILKFPDELEHVESAGKVSAQILKSNLVAMEQSILHLEKNIKNFPPAESHHDKFVEKMMSFTQNAREQYDKLSTMHSNMLKLYESLGEYFIFDPNTVNMEEFFGDLNTFRTLFLEALKENHKRKEMEEKSRRAKLAKEKAEQEKLERQKKKKQLIDINKEGDETGVMDNLLEALQSGAAFRDRRKRIPRNPDNRRPPLERSRSRHNGAMSSK

Target ORF information:

RefSeq Version	NM_172493.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus diaphanous related formin 2 (Diaph2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172493.2

ORF Insert Sequence:

ATGGAGGAGC TCGGGGCGGC GGCGTCGGGA GCCGGAGGCG GCGGCGGTGG CGGCGAGGAA 
CACGGCGGGG GCCGGAGCAA CAAGCGAGGC GCGGGGAACC GGGCCGCCAA CGAAGAGGAG 
ACGAGAAACA AACCCAAACT GAACATTCAA ATAAAAACTT TGGCAGATGA TGTGCGTGAC 
CGAATTACAA GTTTCAGAAA ATCTGCCACC AAGAGAGAAA AGCCTGTTAT TCAACATTCT 
ATTGATTATC AAACTGCAGT GGTTGAGATT CCCCCAGCTC TGATAGTACA TGATGACCGA 
TCTTTGATTT TGTCTGAAAA GGAAGTTCTG GACCTCTTTG AGAAAATGAT GGAAGATATG 
AACCTTAATG AAGAAAAGAA AGCTCCTTTG CGAAAGAAGG ATTTTAGCAT CAAGCGTGAG 
ATGGTTGTCC AGTATATTTC TGCTACTTCC AAATCTGGTG GCCTGAAAAA CAGCAAACAT 
GAGTTCACCC TTTCTTCACA AGAATATGTT CATGAATTAC GATCTGGCAT CTCAGATGAG 
AAACTTCTCA ACTGCCTGGA ATCTTTGAGA GTGTCTTTAA CCAGCCATCC TGTCAGCTGG 
GTCAACAACT TTGGCTATGA AGGACTTGGA GTCTTACTGG ATGTGCTGGA GAAGCTTCTG 
GACAAAAAAC AGCAAGAAAA TATCGACAAG AAGAATCAGT ACAAAGTCAT TCAGTGCCTG 
AAAGCATTTA TGAATAATAA GTTTGGACTA CAAAGAATTC TAGGAGACGA GAGAAGCCTT 
TTACTGTTGG CAAGAGCAAT TGACCCGAAA CAACAGAATA TGATGACTGA AATAGTAAAA 
ATCCTTTCTG CTATTTGCAT TGTTGGGGAA GAAAACATTC TAGACAAACT TTTAGGGGGC 
ATCACTGCTG CAGCTGAACT AAATAATAGA GAACGATTTT CACCAATTGT GGAAGGCTTA 
GAAAATAATG AAGCCTTGCA TCTCCAGGTG GCCTGCATGC AGTTCATAAA TGCACTTGTC 
ACCTCTCCAT ATGATCTTGA TTTTCGAATT CATTTGAGGA ATGAATTCCT TCGTTGTGGA 
TTAAAAGCAA TGCTCCCTAC TCTTAAAGAG ATAGAGAATG AAGGGCTTGA TATTCAGTTG 
AGAGTGTTTG AAGAGAACAA AGAAGATGAC CTAAGTGAAT TATCCCACCG TCTCAATGAC 
ATTCGAGCTG AAATGGATGA TATTAATGAA GTCTACCATC TGCTGTATAA TATGCTGAAG 
GACACTGCTG CGGAACCTTA CTTATTATCC ATTCTCCAAC ACTTTCTGCT CATCAGAAAT 
GATTACTACA TCAGGCCACA GTATTATAAA ATCATTGAGG AGTGTGTTTC ACAGATAGTG 
CTACACTGCA GTGGCATGGA CCCAGACTTC AAGTACCGGC AGCGCATAGA CTTTGATTTC 
ACTCATCTCC TAGATGCTTG TGTAAATAAG GCAAAAGTTG AAGAAAATGA GCAAAAAGCA 
ATGGAATTTT CAAAGAAGTT CGATGAAGAA TTCACAGCTC GTCAGGAAGC TCAAGCAGAG 
CTTCAGAAAA GAGATGAAAA AATCAAAGAA CTGGAAACAG AAATCCAGCA GCTTCGAGGA 
CAGGGAGTTC CATCTGCAAT TCCAGGTCCA CCTCCACCAC CTCCACTGCC AGGTGCAGGA 
CCATGCCCAC CACCACCACC TCCTCCTCCT CCACCACCTC CACTTCCAGG AGTCGTCCCT 
CCTCCTCCTC CACCATTACC TGGGATGCCT GGTATTCCAC CACCACCTCC ACCACCTTTG 
TCTGGAGTAC CTCCTCCACC ACCACCCCCT GGGGGAGTTT TTCCTCTCCT ATCAGGACCA 
ATTGAGCTAC CATATGGAAT GAAGCAGAAA AAATTATATA AGCCTGACAT ACCTATGAAA 
AGAATCAACT GGTCCAAGAT TGAGCCTAAA GAATTATCCG AAAACTGTGT TTGGCTAAAA 
CTTAAAGAAG AGAAGTATGA GAATGCAGAT CTATTTGCAA AACTGGCCTT GACATTTCCT 
TCCCAGATGA AAGGTCAAAG GAATACAGAG GCTGCAGAAG AAAATAGGAG TGGGCCTCCA 
AAGAAGAAAG TGAAGGAATT GAGAATTTTG GATACCAAAA CAGCCCAGAA TCTATCTATC 
TTCCTTGGAT CTTACCGCAT GCCATACGAG GAAATAAAGA ACATTATTTT GGAGGTTAAT 
GAAGAGATGC TCAGTGAGGC CCTAATCCAG AATCTTGTAA AATATCTTCC TGACCAGAAC 
GCACTCCGAG AACTAGCCCA GCTTAAGAGT GAATATGATG ATCTCTGTGA GCCTGAGCAG 
TTTGGTGTTG TGATGAGCAC GGTGAAAATG TTACGTCCTC GTCTCACGAG CATCCTTTTC 
AAACTCACCT TTGAAGAACA TGTCAACAAC ATCAAACCAA GCATCATAGC AGTAACTCTT 
GCATGTGAAG AACTGAAGAA AAGTGAAAGC TTTAAGAGAC TCTTAGAGTT GATTCTTCTG 
GTTGGAAACT ACATGAACTC AGGCTCCAGG AATGCCCAGT CTCTAGGTTT TAAGATTAAC 
TTTCTGTGTA AGATCAAAGA CACAAAATCA GCAGATCAAA AATCCACCCT TTTGCATTTT 
CTTGCTGAAA TCTGTGATGA GAAATACCGG GATATCCTGA AATTTCCTGA TGAGCTGGAG 
CATGTGGAAA GCGCAGGCAA AGTTTCAGCT CAAATACTCA AGAGCAACCT TGTAGCAATG 
GAACAAAGCA TTCTTCATCT GGAGAAAAAC ATCAAGAATT TCCCACCAGC AGAATCGCAC 
CATGATAAGT TTGTGGAAAA GATGATGAGC TTTACCCAGA ATGCCAGAGA ACAGTATGAC 
AAACTGTCCA CCATGCACAG CAATATGTTG AAGCTCTATG AGAGTCTGGG AGAATACTTC 
ATTTTTGACC CGAACACAGT AAACATGGAA GAATTTTTTG GTGATCTCAA CACCTTCAGA 
ACCCTATTTT TGGAAGCACT GAAAGAAAAC CACAAGAGAA AGGAAATGGA AGAGAAGAGC 
AGGAGAGCAA AGCTTGCAAA AGAGAAAGCG GAACAAGAGA AGCTGGAACG CCAGAAGAAG 
AAGAAGCAAC TCATTGACAT AAACAAAGAG GGTGATGAGA CGGGAGTGAT GGATAATCTT 
CTGGAAGCCC TACAGTCAGG TGCAGCATTC AGAGACCGCA GAAAGCGAAT TCCAAGAAAT 
CCAGATAACA GACGGCCCCC TCTGGAAAGA TCTCGCTCTC GCCACAATGG AGCCATGTCA 
TCCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Diaph2 ( XM_006528581.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006528644.1 Mus musculus diaphanous related formin 2 (Diaph2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu56538
Clone ID Related Accession (Same CDS sequence)	XM_006528581.3 , XM_006528581.4
Accession Version	XM_006528581.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3330bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein diaphanous homolog 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006528581.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TCGGGGCGGC GGCGTCGGGA GCCGGAGGCG GCGGCGGTGG CGGCGAGGAA 
CACGGCGGGG GCCGGAGCAA CAAGCGAGGC GCGGGGAACC GGGCCGCCAA CGAAGAGGAG 
ACGAGAAACA AACCCAAACT GAACATTCAA ATAAAAACTT TGGCAGATGA TGTGCGTGAC 
CGAATTACAA GTTTCAGAAA ATCTGCCACC AAGAGAGAAA AGCCTGTTAT TCAACATTCT 
ATTGATTATC AAACTGCAGT GGTTGAGATT CCCCCAGCTC TGATAGTACA TGATGACCGA 
TCTTTGATTT TGTCTGAAAA GGAAGTTCTG GACCTCTTTG AGAAAATGAT GGAAGATATG 
AACCTTAATG AAGAAAAGAA AGCTCCTTTG CGAAAGAAGG ATTTTAGCAT CAAGCGTGAG 
ATGGTTGTCC AGTATATTTC TGCTACTTCC AAATCTATAG TTGGAAGTAA AGTTCTGGGT 
GGCCTGAAAA ACAGCAAACA TGAGTTCACC CTTTCTTCAC AAGAATATGT TCATGAATTA 
CGATCTGGCA TCTCAGATGA GAAACTTCTC AACTGCCTGG AATCTTTGAG AGTGTCTTTA 
ACCAGCCATC CTGTCAGCTG GGTCAACAAC TTTGGCTATG AAGGACTTGG AGTCTTACTG 
GATGTGCTGG AGAAGCTTCT GGACAAAAAA CAGCAAGAAA ATATCGACAA GAAGAATCAG 
TACAAAGTCA TTCAGTGCCT GAAAGCATTT ATGAATAATA AGTTTGGACT ACAAAGAATT 
CTAGGAGACG AGAGAAGCCT TTTACTGTTG GCAAGAGCAA TTGACCCGAA ACAACAGAAT 
ATGATGACTG AAATAGTAAA AATCCTTTCT GCTATTTGCA TTGTTGGGGA AGAAAACATT 
CTAGACAAAC TTTTAGGGGG CATCACTGCT GCAGCTGAAC TAAATAATAG AGAACGATTT 
TCACCAATTG TGGAAGGCTT AGAAAATAAT GAAGCCTTGC ATCTCCAGGT GGCCTGCATG 
CAGTTCATAA ATGCACTTGT CACCTCTCCA TATGATCTTG ATTTTCGAAT TCATTTGAGG 
AATGAATTCC TTCGTTGTGG ATTAAAAGCA ATGCTCCCTA CTCTTAAAGA GATAGAGAAT 
GAAGGGCTTG ATATTCAGTT GAGAGTGTTT GAAGAGAACA AAGAAGATGA CCTAAGTGAA 
TTATCCCACC GTCTCAATGA CATTCGAGCT GAAATGGATG ATATTAATGA AGTCTACCAT 
CTGCTGTATA ATATGCTGAA GGACACTGCT GCGGAACCTT ACTTATTATC CATTCTCCAA 
CACTTTCTGC TCATCAGAAA TGATTACTAC ATCAGGCCAC AGTATTATAA AATCATTGAG 
GAGTGTGTTT CACAGATAGT GCTACACTGC AGTGGCATGG ACCCAGACTT CAAGTACCGG 
CAGCGCATAG ACTTTGATTT CACTCATCTC CTAGATGCTT GTGTAAATAA GGCAAAAGTT 
GAAGAAAATG AGCAAAAAGC AATGGAATTT TCAAAGAAGT TCGATGAAGA ATTCACAGCT 
CGTCAGGAAG CTCAAGCAGA GCTTCAGAAA AGAGATGAAA AAATCAAAGA ACTGGAAACA 
GAAATCCAGC AGCTTCGAGG ACAGGGAGTT CCATCTGCAA TTCCAGGTCC ACCTCCACCA 
CCTCCACTGC CAGGTGCAGG ACCATGCCCA CCACCACCAC CTCCTCCTCC TCCACCACCT 
CCACTTCCAG GAGTCGTCCC TCCTCCTCCT CCACCATTAC CTGGGATGCC TGGTATTCCA 
CCACCACCTC CACCACCTTT GTCTGGAGTA CCTCCTCCAC CACCACCCCC TGGGGGAGTT 
TTTCCTCTCC TATCAGGACC AATTGAGCTA CCATATGGAA TGAAGCAGAA AAAATTATAT 
AAGCCTGACA TACCTATGAA AAGAATCAAC TGGTCCAAGA TTGAGCCTAA AGAATTATCC 
GAAAACTGTG TTTGGCTAAA ACTTAAAGAA GAGAAGTATG AGAATGCAGA TCTATTTGCA 
AAACTGGCCT TGACATTTCC TTCCCAGATG AAAGGTCAAA GGAATACAGA GGCTGCAGAA 
GAAAATAGGA GTGGGCCTCC AAAGAAGAAA GTGAAGGAAT TGAGAATTTT GGATACCAAA 
ACAGCCCAGA ATCTATCTAT CTTCCTTGGA TCTTACCGCA TGCCATACGA GGAAATAAAG 
AACATTATTT TGGAGGTTAA TGAAGAGATG CTCAGTGAGG CCCTAATCCA GAATCTTGTA 
AAATATCTTC CTGACCAGAA CGCACTCCGA GAACTAGCCC AGCTTAAGAG TGAATATGAT 
GATCTCTGTG AGCCTGAGCA GTTTGGTGTT GTGATGAGCA CGGTGAAAAT GTTACGTCCT 
CGTCTCACGA GCATCCTTTT CAAACTCACC TTTGAAGAAC ATGTCAACAA CATCAAACCA 
AGCATCATAG CAGTAACTCT TGCATGTGAA GAACTGAAGA AAAGTGAAAG CTTTAAGAGA 
CTCTTAGAGT TGATTCTTCT GGTTGGAAAC TACATGAACT CAGGCTCCAG GAATGCCCAG 
TCTCTAGGTT TTAAGATTAA CTTTCTGTGT AAGATCAAAG ACACAAAATC AGCAGATCAA 
AAATCCACCC TTTTGCATTT TCTTGCTGAA ATCTGTGATG AGAAATACCG GGATATCCTG 
AAATTTCCTG ATGAGCTGGA GCATGTGGAA AGCGCAGGCA AAGTTTCAGC TCAAATACTC 
AAGAGCAACC TTGTAGCAAT GGAACAAAGC ATTCTTCATC TGGAGAAAAA CATCAAGAAT 
TTCCCACCAG CAGAATCGCA CCATGATAAG TTTGTGGAAA AGATGATGAG CTTTACCCAG 
AATGCCAGAG AACAGTATGA CAAACTGTCC ACCATGCACA GCAATATGTT GAAGCTCTAT 
GAGAGTCTGG GAGAATACTT CATTTTTGAC CCGAACACAG TAAACATGGA AGAATTTTTT 
GGTGATCTCA ACACCTTCAG AACCCTATTT TTGGAAGCAC TGAAAGAAAA CCACAAGAGA 
AAGGAAATGG AAGAGAAGAG CAGGAGAGCA AAGCTTGCAA AAGAGAAAGC GGAACAAGAG 
AAGCTGGAAC GCCAGAAGAA GAAGAAGCAA CTCATTGACA TAAACAAAGA GGGTGATGAG 
ACGGGAGTGA TGGATAATCT TCTGGAAGCC CTACAGTCAG GTGCAGCATT CAGAGACCGC 
AGAAAGCGAA TTCCAAGAAA TCCAGATAAC AGACGGCCCC CTCTGGAAAG ATCTCGCTCT 
CGCCACAATG GAGCCATGTC ATCCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006528644.1
CDS	309..3638
Translation	MEELGAAASGAGGGGGGGEEHGGGRSNKRGAGNRAANEEETRNKPKLNIQIKTLADDVRDRITSFRKSATKREKPVIQHSIDYQTAVVEIPPALIVHDDRSLILSEKEVLDLFEKMMEDMNLNEEKKAPLRKKDFSIKREMVVQYISATSKSIVGSKVLGGLKNSKHEFTLSSQEYVHELRSGISDEKLLNCLESLRVSLTSHPVSWVNNFGYEGLGVLLDVLEKLLDKKQQENIDKKNQYKVIQCLKAFMNNKFGLQRILGDERSLLLLARAIDPKQQNMMTEIVKILSAICIVGEENILDKLLGGITAAAELNNRERFSPIVEGLENNEALHLQVACMQFINALVTSPYDLDFRIHLRNEFLRCGLKAMLPTLKEIENEGLDIQLRVFEENKEDDLSELSHRLNDIRAEMDDINEVYHLLYNMLKDTAAEPYLLSILQHFLLIRNDYYIRPQYYKIIEECVSQIVLHCSGMDPDFKYRQRIDFDFTHLLDACVNKAKVEENEQKAMEFSKKFDEEFTARQEAQAELQKRDEKIKELETEIQQLRGQGVPSAIPGPPPPPPLPGAGPCPPPPPPPPPPPPLPGVVPPPPPPLPGMPGIPPPPPPPLSGVPPPPPPPGGVFPLLSGPIELPYGMKQKKLYKPDIPMKRINWSKIEPKELSENCVWLKLKEEKYENADLFAKLALTFPSQMKGQRNTEAAEENRSGPPKKKVKELRILDTKTAQNLSIFLGSYRMPYEEIKNIILEVNEEMLSEALIQNLVKYLPDQNALRELAQLKSEYDDLCEPEQFGVVMSTVKMLRPRLTSILFKLTFEEHVNNIKPSIIAVTLACEELKKSESFKRLLELILLVGNYMNSGSRNAQSLGFKINFLCKIKDTKSADQKSTLLHFLAEICDEKYRDILKFPDELEHVESAGKVSAQILKSNLVAMEQSILHLEKNIKNFPPAESHHDKFVEKMMSFTQNAREQYDKLSTMHSNMLKLYESLGEYFIFDPNTVNMEEFFGDLNTFRTLFLEALKENHKRKEMEEKSRRAKLAKEKAEQEKLERQKKKKQLIDINKEGDETGVMDNLLEALQSGAAFRDRRKRIPRNPDNRRPPLERSRSRHNGAMSSK

Target ORF information:

RefSeq Version	XM_006528581.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus diaphanous related formin 2 (Diaph2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528581.4

ORF Insert Sequence:

ATGGAGGAGC TCGGGGCGGC GGCGTCGGGA GCCGGAGGCG GCGGCGGTGG CGGCGAGGAA 
CACGGCGGGG GCCGGAGCAA CAAGCGAGGC GCGGGGAACC GGGCCGCCAA CGAAGAGGAG 
ACGAGAAACA AACCCAAACT GAACATTCAA ATAAAAACTT TGGCAGATGA TGTGCGTGAC 
CGAATTACAA GTTTCAGAAA ATCTGCCACC AAGAGAGAAA AGCCTGTTAT TCAACATTCT 
ATTGATTATC AAACTGCAGT GGTTGAGATT CCCCCAGCTC TGATAGTACA TGATGACCGA 
TCTTTGATTT TGTCTGAAAA GGAAGTTCTG GACCTCTTTG AGAAAATGAT GGAAGATATG 
AACCTTAATG AAGAAAAGAA AGCTCCTTTG CGAAAGAAGG ATTTTAGCAT CAAGCGTGAG 
ATGGTTGTCC AGTATATTTC TGCTACTTCC AAATCTATAG TTGGAAGTAA AGTTCTGGGT 
GGCCTGAAAA ACAGCAAACA TGAGTTCACC CTTTCTTCAC AAGAATATGT TCATGAATTA 
CGATCTGGCA TCTCAGATGA GAAACTTCTC AACTGCCTGG AATCTTTGAG AGTGTCTTTA 
ACCAGCCATC CTGTCAGCTG GGTCAACAAC TTTGGCTATG AAGGACTTGG AGTCTTACTG 
GATGTGCTGG AGAAGCTTCT GGACAAAAAA CAGCAAGAAA ATATCGACAA GAAGAATCAG 
TACAAAGTCA TTCAGTGCCT GAAAGCATTT ATGAATAATA AGTTTGGACT ACAAAGAATT 
CTAGGAGACG AGAGAAGCCT TTTACTGTTG GCAAGAGCAA TTGACCCGAA ACAACAGAAT 
ATGATGACTG AAATAGTAAA AATCCTTTCT GCTATTTGCA TTGTTGGGGA AGAAAACATT 
CTAGACAAAC TTTTAGGGGG CATCACTGCT GCAGCTGAAC TAAATAATAG AGAACGATTT 
TCACCAATTG TGGAAGGCTT AGAAAATAAT GAAGCCTTGC ATCTCCAGGT GGCCTGCATG 
CAGTTCATAA ATGCACTTGT CACCTCTCCA TATGATCTTG ATTTTCGAAT TCATTTGAGG 
AATGAATTCC TTCGTTGTGG ATTAAAAGCA ATGCTCCCTA CTCTTAAAGA GATAGAGAAT 
GAAGGGCTTG ATATTCAGTT GAGAGTGTTT GAAGAGAACA AAGAAGATGA CCTAAGTGAA 
TTATCCCACC GTCTCAATGA CATTCGAGCT GAAATGGATG ATATTAATGA AGTCTACCAT 
CTGCTGTATA ATATGCTGAA GGACACTGCT GCGGAACCTT ACTTATTATC CATTCTCCAA 
CACTTTCTGC TCATCAGAAA TGATTACTAC ATCAGGCCAC AGTATTATAA AATCATTGAG 
GAGTGTGTTT CACAGATAGT GCTACACTGC AGTGGCATGG ACCCAGACTT CAAGTACCGG 
CAGCGCATAG ACTTTGATTT CACTCATCTC CTAGATGCTT GTGTAAATAA GGCAAAAGTT 
GAAGAAAATG AGCAAAAAGC AATGGAATTT TCAAAGAAGT TCGATGAAGA ATTCACAGCT 
CGTCAGGAAG CTCAAGCAGA GCTTCAGAAA AGAGATGAAA AAATCAAAGA ACTGGAAACA 
GAAATCCAGC AGCTTCGAGG ACAGGGAGTT CCATCTGCAA TTCCAGGTCC ACCTCCACCA 
CCTCCACTGC CAGGTGCAGG ACCATGCCCA CCACCACCAC CTCCTCCTCC TCCACCACCT 
CCACTTCCAG GAGTCGTCCC TCCTCCTCCT CCACCATTAC CTGGGATGCC TGGTATTCCA 
CCACCACCTC CACCACCTTT GTCTGGAGTA CCTCCTCCAC CACCACCCCC TGGGGGAGTT 
TTTCCTCTCC TATCAGGACC AATTGAGCTA CCATATGGAA TGAAGCAGAA AAAATTATAT 
AAGCCTGACA TACCTATGAA AAGAATCAAC TGGTCCAAGA TTGAGCCTAA AGAATTATCC 
GAAAACTGTG TTTGGCTAAA ACTTAAAGAA GAGAAGTATG AGAATGCAGA TCTATTTGCA 
AAACTGGCCT TGACATTTCC TTCCCAGATG AAAGGTCAAA GGAATACAGA GGCTGCAGAA 
GAAAATAGGA GTGGGCCTCC AAAGAAGAAA GTGAAGGAAT TGAGAATTTT GGATACCAAA 
ACAGCCCAGA ATCTATCTAT CTTCCTTGGA TCTTACCGCA TGCCATACGA GGAAATAAAG 
AACATTATTT TGGAGGTTAA TGAAGAGATG CTCAGTGAGG CCCTAATCCA GAATCTTGTA 
AAATATCTTC CTGACCAGAA CGCACTCCGA GAACTAGCCC AGCTTAAGAG TGAATATGAT 
GATCTCTGTG AGCCTGAGCA GTTTGGTGTT GTGATGAGCA CGGTGAAAAT GTTACGTCCT 
CGTCTCACGA GCATCCTTTT CAAACTCACC TTTGAAGAAC ATGTCAACAA CATCAAACCA 
AGCATCATAG CAGTAACTCT TGCATGTGAA GAACTGAAGA AAAGTGAAAG CTTTAAGAGA 
CTCTTAGAGT TGATTCTTCT GGTTGGAAAC TACATGAACT CAGGCTCCAG GAATGCCCAG 
TCTCTAGGTT TTAAGATTAA CTTTCTGTGT AAGATCAAAG ACACAAAATC AGCAGATCAA 
AAATCCACCC TTTTGCATTT TCTTGCTGAA ATCTGTGATG AGAAATACCG GGATATCCTG 
AAATTTCCTG ATGAGCTGGA GCATGTGGAA AGCGCAGGCA AAGTTTCAGC TCAAATACTC 
AAGAGCAACC TTGTAGCAAT GGAACAAAGC ATTCTTCATC TGGAGAAAAA CATCAAGAAT 
TTCCCACCAG CAGAATCGCA CCATGATAAG TTTGTGGAAA AGATGATGAG CTTTACCCAG 
AATGCCAGAG AACAGTATGA CAAACTGTCC ACCATGCACA GCAATATGTT GAAGCTCTAT 
GAGAGTCTGG GAGAATACTT CATTTTTGAC CCGAACACAG TAAACATGGA AGAATTTTTT 
GGTGATCTCA ACACCTTCAG AACCCTATTT TTGGAAGCAC TGAAAGAAAA CCACAAGAGA 
AAGGAAATGG AAGAGAAGAG CAGGAGAGCA AAGCTTGCAA AAGAGAAAGC GGAACAAGAG 
AAGCTGGAAC GCCAGAAGAA GAAGAAGCAA CTCATTGACA TAAACAAAGA GGGTGATGAG 
ACGGGAGTGA TGGATAATCT TCTGGAAGCC CTACAGTCAG GTGCAGCATT CAGAGACCGC 
AGAAAGCGAA TTCCAAGAAA TCCAGATAAC AGACGGCCCC CTCTGGAAAG ATCTCGCTCT 
CGCCACAATG GAGCCATGTC ATCCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Diaph2 ( XM_006528582.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006528645.1 Mus musculus diaphanous related formin 2 (Diaph2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu56539
Clone ID Related Accession (Same CDS sequence)	XM_006528582.4 , XM_006528582.3
Accession Version	XM_006528582.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein diaphanous homolog 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006528582.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TCGGGGCGGC GGCGTCGGGA GCCGGAGGCG GCGGCGGTGG CGGCGAGGAA 
CACGGCGGGG GCCGGAGCAA CAAGCGAGGC GCGGGGAACC GGGCCGCCAA CGAAGAGGAG 
ACGAGAAACA AACCCAAACT GCGTGACCGA ATTACAAGTT TCAGAAAATC TGCCACCAAG 
AGAGAAAAGC CTGTTATTCA ACATTCTATT GATTATCAAA CTGCAGTGGT TGAGATTCCC 
CCAGCTCTGA TAGTACATGA TGACCGATCT TTGATTTTGT CTGAAAAGGA AGTTCTGGAC 
CTCTTTGAGA AAATGATGGA AGATATGAAC CTTAATGAAG AAAAGAAAGC TCCTTTGCGA 
AAGAAGGATT TTAGCATCAA GCGTGAGATG GTTGTCCAGT ATATTTCTGC TACTTCCAAA 
TCTATAGTTG GAAGTAAAGT TCTGGGTGGC CTGAAAAACA GCAAACATGA GTTCACCCTT 
TCTTCACAAG AATATGTTCA TGAATTACGA TCTGGCATCT CAGATGAGAA ACTTCTCAAC 
TGCCTGGAAT CTTTGAGAGT GTCTTTAACC AGCCATCCTG TCAGCTGGGT CAACAACTTT 
GGCTATGAAG GACTTGGAGT CTTACTGGAT GTGCTGGAGA AGCTTCTGGA CAAAAAACAG 
CAAGAAAATA TCGACAAGAA GAATCAGTAC AAAGTCATTC AGTGCCTGAA AGCATTTATG 
AATAATAAGT TTGGACTACA AAGAATTCTA GGAGACGAGA GAAGCCTTTT ACTGTTGGCA 
AGAGCAATTG ACCCGAAACA ACAGAATATG ATGACTGAAA TAGTAAAAAT CCTTTCTGCT 
ATTTGCATTG TTGGGGAAGA AAACATTCTA GACAAACTTT TAGGGGGCAT CACTGCTGCA 
GCTGAACTAA ATAATAGAGA ACGATTTTCA CCAATTGTGG AAGGCTTAGA AAATAATGAA 
GCCTTGCATC TCCAGGTGGC CTGCATGCAG TTCATAAATG CACTTGTCAC CTCTCCATAT 
GATCTTGATT TTCGAATTCA TTTGAGGAAT GAATTCCTTC GTTGTGGATT AAAAGCAATG 
CTCCCTACTC TTAAAGAGAT AGAGAATGAA GGGCTTGATA TTCAGTTGAG AGTGTTTGAA 
GAGAACAAAG AAGATGACCT AAGTGAATTA TCCCACCGTC TCAATGACAT TCGAGCTGAA 
ATGGATGATA TTAATGAAGT CTACCATCTG CTGTATAATA TGCTGAAGGA CACTGCTGCG 
GAACCTTACT TATTATCCAT TCTCCAACAC TTTCTGCTCA TCAGAAATGA TTACTACATC 
AGGCCACAGT ATTATAAAAT CATTGAGGAG TGTGTTTCAC AGATAGTGCT ACACTGCAGT 
GGCATGGACC CAGACTTCAA GTACCGGCAG CGCATAGACT TTGATTTCAC TCATCTCCTA 
GATGCTTGTG TAAATAAGGC AAAAGTTGAA GAAAATGAGC AAAAAGCAAT GGAATTTTCA 
AAGAAGTTCG ATGAAGAATT CACAGCTCGT CAGGAAGCTC AAGCAGAGCT TCAGAAAAGA 
GATGAAAAAA TCAAAGAACT GGAAACAGAA ATCCAGCAGC TTCGAGGACA GGGAGTTCCA 
TCTGCAATTC CAGGTCCACC TCCACCACCT CCACTGCCAG GTGCAGGACC ATGCCCACCA 
CCACCACCTC CTCCTCCTCC ACCACCTCCA CTTCCAGGAG TCGTCCCTCC TCCTCCTCCA 
CCATTACCTG GGATGCCTGG TATTCCACCA CCACCTCCAC CACCTTTGTC TGGAGTACCT 
CCTCCACCAC CACCCCCTGG GGGAGTTTTT CCTCTCCTAT CAGGACCAAT TGAGCTACCA 
TATGGAATGA AGCAGAAAAA ATTATATAAG CCTGACATAC CTATGAAAAG AATCAACTGG 
TCCAAGATTG AGCCTAAAGA ATTATCCGAA AACTGTGTTT GGCTAAAACT TAAAGAAGAG 
AAGTATGAGA ATGCAGATCT ATTTGCAAAA CTGGCCTTGA CATTTCCTTC CCAGATGAAA 
GGTCAAAGGA ATACAGAGGC TGCAGAAGAA AATAGGAGTG GGCCTCCAAA GAAGAAAGTG 
AAGGAATTGA GAATTTTGGA TACCAAAACA GCCCAGAATC TATCTATCTT CCTTGGATCT 
TACCGCATGC CATACGAGGA AATAAAGAAC ATTATTTTGG AGGTTAATGA AGAGATGCTC 
AGTGAGGCCC TAATCCAGAA TCTTGTAAAA TATCTTCCTG ACCAGAACGC ACTCCGAGAA 
CTAGCCCAGC TTAAGAGTGA ATATGATGAT CTCTGTGAGC CTGAGCAGTT TGGTGTTGTG 
ATGAGCACGG TGAAAATGTT ACGTCCTCGT CTCACGAGCA TCCTTTTCAA ACTCACCTTT 
GAAGAACATG TCAACAACAT CAAACCAAGC ATCATAGCAG TAACTCTTGC ATGTGAAGAA 
CTGAAGAAAA GTGAAAGCTT TAAGAGACTC TTAGAGTTGA TTCTTCTGGT TGGAAACTAC 
ATGAACTCAG GCTCCAGGAA TGCCCAGTCT CTAGGTTTTA AGATTAACTT TCTGTGTAAG 
ATCAAAGACA CAAAATCAGC AGATCAAAAA TCCACCCTTT TGCATTTTCT TGCTGAAATC 
TGTGATGAGA AATACCGGGA TATCCTGAAA TTTCCTGATG AGCTGGAGCA TGTGGAAAGC 
GCAGGCAAAG TTTCAGCTCA AATACTCAAG AGCAACCTTG TAGCAATGGA ACAAAGCATT 
CTTCATCTGG AGAAAAACAT CAAGAATTTC CCACCAGCAG AATCGCACCA TGATAAGTTT 
GTGGAAAAGA TGATGAGCTT TACCCAGAAT GCCAGAGAAC AGTATGACAA ACTGTCCACC 
ATGCACAGCA ATATGTTGAA GCTCTATGAG AGTCTGGGAG AATACTTCAT TTTTGACCCG 
AACACAGTAA ACATGGAAGA ATTTTTTGGT GATCTCAACA CCTTCAGAAC CCTATTTTTG 
GAAGCACTGA AAGAAAACCA CAAGAGAAAG GAAATGGAAG AGAAGAGCAG GAGAGCAAAG 
CTTGCAAAAG AGAAAGCGGA ACAAGAGAAG CTGGAACGCC AGAAGAAGAA GAAGCAACTC 
ATTGACATAA ACAAAGAGGG TGATGAGACG GGAGTGATGG ATAATCTTCT GGAAGCCCTA 
CAGTCAGGTG CAGCATTCAG AGACCGCAGA AAGCGAATTC CAAGAAATCC AGATAACAGA 
CGGCCCCCTC TGGAAAGATC TCGCTCTCGC CACAATGGAG CCATGTCATC CAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006528645.1
CDS	309..3605
Translation	MEELGAAASGAGGGGGGGEEHGGGRSNKRGAGNRAANEEETRNKPKLRDRITSFRKSATKREKPVIQHSIDYQTAVVEIPPALIVHDDRSLILSEKEVLDLFEKMMEDMNLNEEKKAPLRKKDFSIKREMVVQYISATSKSIVGSKVLGGLKNSKHEFTLSSQEYVHELRSGISDEKLLNCLESLRVSLTSHPVSWVNNFGYEGLGVLLDVLEKLLDKKQQENIDKKNQYKVIQCLKAFMNNKFGLQRILGDERSLLLLARAIDPKQQNMMTEIVKILSAICIVGEENILDKLLGGITAAAELNNRERFSPIVEGLENNEALHLQVACMQFINALVTSPYDLDFRIHLRNEFLRCGLKAMLPTLKEIENEGLDIQLRVFEENKEDDLSELSHRLNDIRAEMDDINEVYHLLYNMLKDTAAEPYLLSILQHFLLIRNDYYIRPQYYKIIEECVSQIVLHCSGMDPDFKYRQRIDFDFTHLLDACVNKAKVEENEQKAMEFSKKFDEEFTARQEAQAELQKRDEKIKELETEIQQLRGQGVPSAIPGPPPPPPLPGAGPCPPPPPPPPPPPPLPGVVPPPPPPLPGMPGIPPPPPPPLSGVPPPPPPPGGVFPLLSGPIELPYGMKQKKLYKPDIPMKRINWSKIEPKELSENCVWLKLKEEKYENADLFAKLALTFPSQMKGQRNTEAAEENRSGPPKKKVKELRILDTKTAQNLSIFLGSYRMPYEEIKNIILEVNEEMLSEALIQNLVKYLPDQNALRELAQLKSEYDDLCEPEQFGVVMSTVKMLRPRLTSILFKLTFEEHVNNIKPSIIAVTLACEELKKSESFKRLLELILLVGNYMNSGSRNAQSLGFKINFLCKIKDTKSADQKSTLLHFLAEICDEKYRDILKFPDELEHVESAGKVSAQILKSNLVAMEQSILHLEKNIKNFPPAESHHDKFVEKMMSFTQNAREQYDKLSTMHSNMLKLYESLGEYFIFDPNTVNMEEFFGDLNTFRTLFLEALKENHKRKEMEEKSRRAKLAKEKAEQEKLERQKKKKQLIDINKEGDETGVMDNLLEALQSGAAFRDRRKRIPRNPDNRRPPLERSRSRHNGAMSSK

Target ORF information:

RefSeq Version	XM_006528582.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus diaphanous related formin 2 (Diaph2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528582.4

ORF Insert Sequence:

ATGGAGGAGC TCGGGGCGGC GGCGTCGGGA GCCGGAGGCG GCGGCGGTGG CGGCGAGGAA 
CACGGCGGGG GCCGGAGCAA CAAGCGAGGC GCGGGGAACC GGGCCGCCAA CGAAGAGGAG 
ACGAGAAACA AACCCAAACT GCGTGACCGA ATTACAAGTT TCAGAAAATC TGCCACCAAG 
AGAGAAAAGC CTGTTATTCA ACATTCTATT GATTATCAAA CTGCAGTGGT TGAGATTCCC 
CCAGCTCTGA TAGTACATGA TGACCGATCT TTGATTTTGT CTGAAAAGGA AGTTCTGGAC 
CTCTTTGAGA AAATGATGGA AGATATGAAC CTTAATGAAG AAAAGAAAGC TCCTTTGCGA 
AAGAAGGATT TTAGCATCAA GCGTGAGATG GTTGTCCAGT ATATTTCTGC TACTTCCAAA 
TCTATAGTTG GAAGTAAAGT TCTGGGTGGC CTGAAAAACA GCAAACATGA GTTCACCCTT 
TCTTCACAAG AATATGTTCA TGAATTACGA TCTGGCATCT CAGATGAGAA ACTTCTCAAC 
TGCCTGGAAT CTTTGAGAGT GTCTTTAACC AGCCATCCTG TCAGCTGGGT CAACAACTTT 
GGCTATGAAG GACTTGGAGT CTTACTGGAT GTGCTGGAGA AGCTTCTGGA CAAAAAACAG 
CAAGAAAATA TCGACAAGAA GAATCAGTAC AAAGTCATTC AGTGCCTGAA AGCATTTATG 
AATAATAAGT TTGGACTACA AAGAATTCTA GGAGACGAGA GAAGCCTTTT ACTGTTGGCA 
AGAGCAATTG ACCCGAAACA ACAGAATATG ATGACTGAAA TAGTAAAAAT CCTTTCTGCT 
ATTTGCATTG TTGGGGAAGA AAACATTCTA GACAAACTTT TAGGGGGCAT CACTGCTGCA 
GCTGAACTAA ATAATAGAGA ACGATTTTCA CCAATTGTGG AAGGCTTAGA AAATAATGAA 
GCCTTGCATC TCCAGGTGGC CTGCATGCAG TTCATAAATG CACTTGTCAC CTCTCCATAT 
GATCTTGATT TTCGAATTCA TTTGAGGAAT GAATTCCTTC GTTGTGGATT AAAAGCAATG 
CTCCCTACTC TTAAAGAGAT AGAGAATGAA GGGCTTGATA TTCAGTTGAG AGTGTTTGAA 
GAGAACAAAG AAGATGACCT AAGTGAATTA TCCCACCGTC TCAATGACAT TCGAGCTGAA 
ATGGATGATA TTAATGAAGT CTACCATCTG CTGTATAATA TGCTGAAGGA CACTGCTGCG 
GAACCTTACT TATTATCCAT TCTCCAACAC TTTCTGCTCA TCAGAAATGA TTACTACATC 
AGGCCACAGT ATTATAAAAT CATTGAGGAG TGTGTTTCAC AGATAGTGCT ACACTGCAGT 
GGCATGGACC CAGACTTCAA GTACCGGCAG CGCATAGACT TTGATTTCAC TCATCTCCTA 
GATGCTTGTG TAAATAAGGC AAAAGTTGAA GAAAATGAGC AAAAAGCAAT GGAATTTTCA 
AAGAAGTTCG ATGAAGAATT CACAGCTCGT CAGGAAGCTC AAGCAGAGCT TCAGAAAAGA 
GATGAAAAAA TCAAAGAACT GGAAACAGAA ATCCAGCAGC TTCGAGGACA GGGAGTTCCA 
TCTGCAATTC CAGGTCCACC TCCACCACCT CCACTGCCAG GTGCAGGACC ATGCCCACCA 
CCACCACCTC CTCCTCCTCC ACCACCTCCA CTTCCAGGAG TCGTCCCTCC TCCTCCTCCA 
CCATTACCTG GGATGCCTGG TATTCCACCA CCACCTCCAC CACCTTTGTC TGGAGTACCT 
CCTCCACCAC CACCCCCTGG GGGAGTTTTT CCTCTCCTAT CAGGACCAAT TGAGCTACCA 
TATGGAATGA AGCAGAAAAA ATTATATAAG CCTGACATAC CTATGAAAAG AATCAACTGG 
TCCAAGATTG AGCCTAAAGA ATTATCCGAA AACTGTGTTT GGCTAAAACT TAAAGAAGAG 
AAGTATGAGA ATGCAGATCT ATTTGCAAAA CTGGCCTTGA CATTTCCTTC CCAGATGAAA 
GGTCAAAGGA ATACAGAGGC TGCAGAAGAA AATAGGAGTG GGCCTCCAAA GAAGAAAGTG 
AAGGAATTGA GAATTTTGGA TACCAAAACA GCCCAGAATC TATCTATCTT CCTTGGATCT 
TACCGCATGC CATACGAGGA AATAAAGAAC ATTATTTTGG AGGTTAATGA AGAGATGCTC 
AGTGAGGCCC TAATCCAGAA TCTTGTAAAA TATCTTCCTG ACCAGAACGC ACTCCGAGAA 
CTAGCCCAGC TTAAGAGTGA ATATGATGAT CTCTGTGAGC CTGAGCAGTT TGGTGTTGTG 
ATGAGCACGG TGAAAATGTT ACGTCCTCGT CTCACGAGCA TCCTTTTCAA ACTCACCTTT 
GAAGAACATG TCAACAACAT CAAACCAAGC ATCATAGCAG TAACTCTTGC ATGTGAAGAA 
CTGAAGAAAA GTGAAAGCTT TAAGAGACTC TTAGAGTTGA TTCTTCTGGT TGGAAACTAC 
ATGAACTCAG GCTCCAGGAA TGCCCAGTCT CTAGGTTTTA AGATTAACTT TCTGTGTAAG 
ATCAAAGACA CAAAATCAGC AGATCAAAAA TCCACCCTTT TGCATTTTCT TGCTGAAATC 
TGTGATGAGA AATACCGGGA TATCCTGAAA TTTCCTGATG AGCTGGAGCA TGTGGAAAGC 
GCAGGCAAAG TTTCAGCTCA AATACTCAAG AGCAACCTTG TAGCAATGGA ACAAAGCATT 
CTTCATCTGG AGAAAAACAT CAAGAATTTC CCACCAGCAG AATCGCACCA TGATAAGTTT 
GTGGAAAAGA TGATGAGCTT TACCCAGAAT GCCAGAGAAC AGTATGACAA ACTGTCCACC 
ATGCACAGCA ATATGTTGAA GCTCTATGAG AGTCTGGGAG AATACTTCAT TTTTGACCCG 
AACACAGTAA ACATGGAAGA ATTTTTTGGT GATCTCAACA CCTTCAGAAC CCTATTTTTG 
GAAGCACTGA AAGAAAACCA CAAGAGAAAG GAAATGGAAG AGAAGAGCAG GAGAGCAAAG 
CTTGCAAAAG AGAAAGCGGA ACAAGAGAAG CTGGAACGCC AGAAGAAGAA GAAGCAACTC 
ATTGACATAA ACAAAGAGGG TGATGAGACG GGAGTGATGG ATAATCTTCT GGAAGCCCTA 
CAGTCAGGTG CAGCATTCAG AGACCGCAGA AAGCGAATTC CAAGAAATCC AGATAACAGA 
CGGCCCCCTC TGGAAAGATC TCGCTCTCGC CACAATGGAG CCATGTCATC CAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Diaph2 ( XM_006528581.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006528644.1 Mus musculus diaphanous related formin 2 (Diaph2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu56538
Clone ID Related Accession (Same CDS sequence)	XM_006528581.3 , XM_006528581.4
Accession Version	XM_006528581.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3330bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein diaphanous homolog 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006528581.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TCGGGGCGGC GGCGTCGGGA GCCGGAGGCG GCGGCGGTGG CGGCGAGGAA 
CACGGCGGGG GCCGGAGCAA CAAGCGAGGC GCGGGGAACC GGGCCGCCAA CGAAGAGGAG 
ACGAGAAACA AACCCAAACT GAACATTCAA ATAAAAACTT TGGCAGATGA TGTGCGTGAC 
CGAATTACAA GTTTCAGAAA ATCTGCCACC AAGAGAGAAA AGCCTGTTAT TCAACATTCT 
ATTGATTATC AAACTGCAGT GGTTGAGATT CCCCCAGCTC TGATAGTACA TGATGACCGA 
TCTTTGATTT TGTCTGAAAA GGAAGTTCTG GACCTCTTTG AGAAAATGAT GGAAGATATG 
AACCTTAATG AAGAAAAGAA AGCTCCTTTG CGAAAGAAGG ATTTTAGCAT CAAGCGTGAG 
ATGGTTGTCC AGTATATTTC TGCTACTTCC AAATCTATAG TTGGAAGTAA AGTTCTGGGT 
GGCCTGAAAA ACAGCAAACA TGAGTTCACC CTTTCTTCAC AAGAATATGT TCATGAATTA 
CGATCTGGCA TCTCAGATGA GAAACTTCTC AACTGCCTGG AATCTTTGAG AGTGTCTTTA 
ACCAGCCATC CTGTCAGCTG GGTCAACAAC TTTGGCTATG AAGGACTTGG AGTCTTACTG 
GATGTGCTGG AGAAGCTTCT GGACAAAAAA CAGCAAGAAA ATATCGACAA GAAGAATCAG 
TACAAAGTCA TTCAGTGCCT GAAAGCATTT ATGAATAATA AGTTTGGACT ACAAAGAATT 
CTAGGAGACG AGAGAAGCCT TTTACTGTTG GCAAGAGCAA TTGACCCGAA ACAACAGAAT 
ATGATGACTG AAATAGTAAA AATCCTTTCT GCTATTTGCA TTGTTGGGGA AGAAAACATT 
CTAGACAAAC TTTTAGGGGG CATCACTGCT GCAGCTGAAC TAAATAATAG AGAACGATTT 
TCACCAATTG TGGAAGGCTT AGAAAATAAT GAAGCCTTGC ATCTCCAGGT GGCCTGCATG 
CAGTTCATAA ATGCACTTGT CACCTCTCCA TATGATCTTG ATTTTCGAAT TCATTTGAGG 
AATGAATTCC TTCGTTGTGG ATTAAAAGCA ATGCTCCCTA CTCTTAAAGA GATAGAGAAT 
GAAGGGCTTG ATATTCAGTT GAGAGTGTTT GAAGAGAACA AAGAAGATGA CCTAAGTGAA 
TTATCCCACC GTCTCAATGA CATTCGAGCT GAAATGGATG ATATTAATGA AGTCTACCAT 
CTGCTGTATA ATATGCTGAA GGACACTGCT GCGGAACCTT ACTTATTATC CATTCTCCAA 
CACTTTCTGC TCATCAGAAA TGATTACTAC ATCAGGCCAC AGTATTATAA AATCATTGAG 
GAGTGTGTTT CACAGATAGT GCTACACTGC AGTGGCATGG ACCCAGACTT CAAGTACCGG 
CAGCGCATAG ACTTTGATTT CACTCATCTC CTAGATGCTT GTGTAAATAA GGCAAAAGTT 
GAAGAAAATG AGCAAAAAGC AATGGAATTT TCAAAGAAGT TCGATGAAGA ATTCACAGCT 
CGTCAGGAAG CTCAAGCAGA GCTTCAGAAA AGAGATGAAA AAATCAAAGA ACTGGAAACA 
GAAATCCAGC AGCTTCGAGG ACAGGGAGTT CCATCTGCAA TTCCAGGTCC ACCTCCACCA 
CCTCCACTGC CAGGTGCAGG ACCATGCCCA CCACCACCAC CTCCTCCTCC TCCACCACCT 
CCACTTCCAG GAGTCGTCCC TCCTCCTCCT CCACCATTAC CTGGGATGCC TGGTATTCCA 
CCACCACCTC CACCACCTTT GTCTGGAGTA CCTCCTCCAC CACCACCCCC TGGGGGAGTT 
TTTCCTCTCC TATCAGGACC AATTGAGCTA CCATATGGAA TGAAGCAGAA AAAATTATAT 
AAGCCTGACA TACCTATGAA AAGAATCAAC TGGTCCAAGA TTGAGCCTAA AGAATTATCC 
GAAAACTGTG TTTGGCTAAA ACTTAAAGAA GAGAAGTATG AGAATGCAGA TCTATTTGCA 
AAACTGGCCT TGACATTTCC TTCCCAGATG AAAGGTCAAA GGAATACAGA GGCTGCAGAA 
GAAAATAGGA GTGGGCCTCC AAAGAAGAAA GTGAAGGAAT TGAGAATTTT GGATACCAAA 
ACAGCCCAGA ATCTATCTAT CTTCCTTGGA TCTTACCGCA TGCCATACGA GGAAATAAAG 
AACATTATTT TGGAGGTTAA TGAAGAGATG CTCAGTGAGG CCCTAATCCA GAATCTTGTA 
AAATATCTTC CTGACCAGAA CGCACTCCGA GAACTAGCCC AGCTTAAGAG TGAATATGAT 
GATCTCTGTG AGCCTGAGCA GTTTGGTGTT GTGATGAGCA CGGTGAAAAT GTTACGTCCT 
CGTCTCACGA GCATCCTTTT CAAACTCACC TTTGAAGAAC ATGTCAACAA CATCAAACCA 
AGCATCATAG CAGTAACTCT TGCATGTGAA GAACTGAAGA AAAGTGAAAG CTTTAAGAGA 
CTCTTAGAGT TGATTCTTCT GGTTGGAAAC TACATGAACT CAGGCTCCAG GAATGCCCAG 
TCTCTAGGTT TTAAGATTAA CTTTCTGTGT AAGATCAAAG ACACAAAATC AGCAGATCAA 
AAATCCACCC TTTTGCATTT TCTTGCTGAA ATCTGTGATG AGAAATACCG GGATATCCTG 
AAATTTCCTG ATGAGCTGGA GCATGTGGAA AGCGCAGGCA AAGTTTCAGC TCAAATACTC 
AAGAGCAACC TTGTAGCAAT GGAACAAAGC ATTCTTCATC TGGAGAAAAA CATCAAGAAT 
TTCCCACCAG CAGAATCGCA CCATGATAAG TTTGTGGAAA AGATGATGAG CTTTACCCAG 
AATGCCAGAG AACAGTATGA CAAACTGTCC ACCATGCACA GCAATATGTT GAAGCTCTAT 
GAGAGTCTGG GAGAATACTT CATTTTTGAC CCGAACACAG TAAACATGGA AGAATTTTTT 
GGTGATCTCA ACACCTTCAG AACCCTATTT TTGGAAGCAC TGAAAGAAAA CCACAAGAGA 
AAGGAAATGG AAGAGAAGAG CAGGAGAGCA AAGCTTGCAA AAGAGAAAGC GGAACAAGAG 
AAGCTGGAAC GCCAGAAGAA GAAGAAGCAA CTCATTGACA TAAACAAAGA GGGTGATGAG 
ACGGGAGTGA TGGATAATCT TCTGGAAGCC CTACAGTCAG GTGCAGCATT CAGAGACCGC 
AGAAAGCGAA TTCCAAGAAA TCCAGATAAC AGACGGCCCC CTCTGGAAAG ATCTCGCTCT 
CGCCACAATG GAGCCATGTC ATCCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006528644.1
CDS	312..3641
Translation	MEELGAAASGAGGGGGGGEEHGGGRSNKRGAGNRAANEEETRNKPKLNIQIKTLADDVRDRITSFRKSATKREKPVIQHSIDYQTAVVEIPPALIVHDDRSLILSEKEVLDLFEKMMEDMNLNEEKKAPLRKKDFSIKREMVVQYISATSKSIVGSKVLGGLKNSKHEFTLSSQEYVHELRSGISDEKLLNCLESLRVSLTSHPVSWVNNFGYEGLGVLLDVLEKLLDKKQQENIDKKNQYKVIQCLKAFMNNKFGLQRILGDERSLLLLARAIDPKQQNMMTEIVKILSAICIVGEENILDKLLGGITAAAELNNRERFSPIVEGLENNEALHLQVACMQFINALVTSPYDLDFRIHLRNEFLRCGLKAMLPTLKEIENEGLDIQLRVFEENKEDDLSELSHRLNDIRAEMDDINEVYHLLYNMLKDTAAEPYLLSILQHFLLIRNDYYIRPQYYKIIEECVSQIVLHCSGMDPDFKYRQRIDFDFTHLLDACVNKAKVEENEQKAMEFSKKFDEEFTARQEAQAELQKRDEKIKELETEIQQLRGQGVPSAIPGPPPPPPLPGAGPCPPPPPPPPPPPPLPGVVPPPPPPLPGMPGIPPPPPPPLSGVPPPPPPPGGVFPLLSGPIELPYGMKQKKLYKPDIPMKRINWSKIEPKELSENCVWLKLKEEKYENADLFAKLALTFPSQMKGQRNTEAAEENRSGPPKKKVKELRILDTKTAQNLSIFLGSYRMPYEEIKNIILEVNEEMLSEALIQNLVKYLPDQNALRELAQLKSEYDDLCEPEQFGVVMSTVKMLRPRLTSILFKLTFEEHVNNIKPSIIAVTLACEELKKSESFKRLLELILLVGNYMNSGSRNAQSLGFKINFLCKIKDTKSADQKSTLLHFLAEICDEKYRDILKFPDELEHVESAGKVSAQILKSNLVAMEQSILHLEKNIKNFPPAESHHDKFVEKMMSFTQNAREQYDKLSTMHSNMLKLYESLGEYFIFDPNTVNMEEFFGDLNTFRTLFLEALKENHKRKEMEEKSRRAKLAKEKAEQEKLERQKKKKQLIDINKEGDETGVMDNLLEALQSGAAFRDRRKRIPRNPDNRRPPLERSRSRHNGAMSSK

Target ORF information:

RefSeq Version	XM_006528581.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus diaphanous related formin 2 (Diaph2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528581.3

ORF Insert Sequence:

ATGGAGGAGC TCGGGGCGGC GGCGTCGGGA GCCGGAGGCG GCGGCGGTGG CGGCGAGGAA 
CACGGCGGGG GCCGGAGCAA CAAGCGAGGC GCGGGGAACC GGGCCGCCAA CGAAGAGGAG 
ACGAGAAACA AACCCAAACT GAACATTCAA ATAAAAACTT TGGCAGATGA TGTGCGTGAC 
CGAATTACAA GTTTCAGAAA ATCTGCCACC AAGAGAGAAA AGCCTGTTAT TCAACATTCT 
ATTGATTATC AAACTGCAGT GGTTGAGATT CCCCCAGCTC TGATAGTACA TGATGACCGA 
TCTTTGATTT TGTCTGAAAA GGAAGTTCTG GACCTCTTTG AGAAAATGAT GGAAGATATG 
AACCTTAATG AAGAAAAGAA AGCTCCTTTG CGAAAGAAGG ATTTTAGCAT CAAGCGTGAG 
ATGGTTGTCC AGTATATTTC TGCTACTTCC AAATCTATAG TTGGAAGTAA AGTTCTGGGT 
GGCCTGAAAA ACAGCAAACA TGAGTTCACC CTTTCTTCAC AAGAATATGT TCATGAATTA 
CGATCTGGCA TCTCAGATGA GAAACTTCTC AACTGCCTGG AATCTTTGAG AGTGTCTTTA 
ACCAGCCATC CTGTCAGCTG GGTCAACAAC TTTGGCTATG AAGGACTTGG AGTCTTACTG 
GATGTGCTGG AGAAGCTTCT GGACAAAAAA CAGCAAGAAA ATATCGACAA GAAGAATCAG 
TACAAAGTCA TTCAGTGCCT GAAAGCATTT ATGAATAATA AGTTTGGACT ACAAAGAATT 
CTAGGAGACG AGAGAAGCCT TTTACTGTTG GCAAGAGCAA TTGACCCGAA ACAACAGAAT 
ATGATGACTG AAATAGTAAA AATCCTTTCT GCTATTTGCA TTGTTGGGGA AGAAAACATT 
CTAGACAAAC TTTTAGGGGG CATCACTGCT GCAGCTGAAC TAAATAATAG AGAACGATTT 
TCACCAATTG TGGAAGGCTT AGAAAATAAT GAAGCCTTGC ATCTCCAGGT GGCCTGCATG 
CAGTTCATAA ATGCACTTGT CACCTCTCCA TATGATCTTG ATTTTCGAAT TCATTTGAGG 
AATGAATTCC TTCGTTGTGG ATTAAAAGCA ATGCTCCCTA CTCTTAAAGA GATAGAGAAT 
GAAGGGCTTG ATATTCAGTT GAGAGTGTTT GAAGAGAACA AAGAAGATGA CCTAAGTGAA 
TTATCCCACC GTCTCAATGA CATTCGAGCT GAAATGGATG ATATTAATGA AGTCTACCAT 
CTGCTGTATA ATATGCTGAA GGACACTGCT GCGGAACCTT ACTTATTATC CATTCTCCAA 
CACTTTCTGC TCATCAGAAA TGATTACTAC ATCAGGCCAC AGTATTATAA AATCATTGAG 
GAGTGTGTTT CACAGATAGT GCTACACTGC AGTGGCATGG ACCCAGACTT CAAGTACCGG 
CAGCGCATAG ACTTTGATTT CACTCATCTC CTAGATGCTT GTGTAAATAA GGCAAAAGTT 
GAAGAAAATG AGCAAAAAGC AATGGAATTT TCAAAGAAGT TCGATGAAGA ATTCACAGCT 
CGTCAGGAAG CTCAAGCAGA GCTTCAGAAA AGAGATGAAA AAATCAAAGA ACTGGAAACA 
GAAATCCAGC AGCTTCGAGG ACAGGGAGTT CCATCTGCAA TTCCAGGTCC ACCTCCACCA 
CCTCCACTGC CAGGTGCAGG ACCATGCCCA CCACCACCAC CTCCTCCTCC TCCACCACCT 
CCACTTCCAG GAGTCGTCCC TCCTCCTCCT CCACCATTAC CTGGGATGCC TGGTATTCCA 
CCACCACCTC CACCACCTTT GTCTGGAGTA CCTCCTCCAC CACCACCCCC TGGGGGAGTT 
TTTCCTCTCC TATCAGGACC AATTGAGCTA CCATATGGAA TGAAGCAGAA AAAATTATAT 
AAGCCTGACA TACCTATGAA AAGAATCAAC TGGTCCAAGA TTGAGCCTAA AGAATTATCC 
GAAAACTGTG TTTGGCTAAA ACTTAAAGAA GAGAAGTATG AGAATGCAGA TCTATTTGCA 
AAACTGGCCT TGACATTTCC TTCCCAGATG AAAGGTCAAA GGAATACAGA GGCTGCAGAA 
GAAAATAGGA GTGGGCCTCC AAAGAAGAAA GTGAAGGAAT TGAGAATTTT GGATACCAAA 
ACAGCCCAGA ATCTATCTAT CTTCCTTGGA TCTTACCGCA TGCCATACGA GGAAATAAAG 
AACATTATTT TGGAGGTTAA TGAAGAGATG CTCAGTGAGG CCCTAATCCA GAATCTTGTA 
AAATATCTTC CTGACCAGAA CGCACTCCGA GAACTAGCCC AGCTTAAGAG TGAATATGAT 
GATCTCTGTG AGCCTGAGCA GTTTGGTGTT GTGATGAGCA CGGTGAAAAT GTTACGTCCT 
CGTCTCACGA GCATCCTTTT CAAACTCACC TTTGAAGAAC ATGTCAACAA CATCAAACCA 
AGCATCATAG CAGTAACTCT TGCATGTGAA GAACTGAAGA AAAGTGAAAG CTTTAAGAGA 
CTCTTAGAGT TGATTCTTCT GGTTGGAAAC TACATGAACT CAGGCTCCAG GAATGCCCAG 
TCTCTAGGTT TTAAGATTAA CTTTCTGTGT AAGATCAAAG ACACAAAATC AGCAGATCAA 
AAATCCACCC TTTTGCATTT TCTTGCTGAA ATCTGTGATG AGAAATACCG GGATATCCTG 
AAATTTCCTG ATGAGCTGGA GCATGTGGAA AGCGCAGGCA AAGTTTCAGC TCAAATACTC 
AAGAGCAACC TTGTAGCAAT GGAACAAAGC ATTCTTCATC TGGAGAAAAA CATCAAGAAT 
TTCCCACCAG CAGAATCGCA CCATGATAAG TTTGTGGAAA AGATGATGAG CTTTACCCAG 
AATGCCAGAG AACAGTATGA CAAACTGTCC ACCATGCACA GCAATATGTT GAAGCTCTAT 
GAGAGTCTGG GAGAATACTT CATTTTTGAC CCGAACACAG TAAACATGGA AGAATTTTTT 
GGTGATCTCA ACACCTTCAG AACCCTATTT TTGGAAGCAC TGAAAGAAAA CCACAAGAGA 
AAGGAAATGG AAGAGAAGAG CAGGAGAGCA AAGCTTGCAA AAGAGAAAGC GGAACAAGAG 
AAGCTGGAAC GCCAGAAGAA GAAGAAGCAA CTCATTGACA TAAACAAAGA GGGTGATGAG 
ACGGGAGTGA TGGATAATCT TCTGGAAGCC CTACAGTCAG GTGCAGCATT CAGAGACCGC 
AGAAAGCGAA TTCCAAGAAA TCCAGATAAC AGACGGCCCC CTCTGGAAAG ATCTCGCTCT 
CGCCACAATG GAGCCATGTC ATCCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Diaph2 ( XM_006528582.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006528645.1 Mus musculus diaphanous related formin 2 (Diaph2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu56539
Clone ID Related Accession (Same CDS sequence)	XM_006528582.4 , XM_006528582.3
Accession Version	XM_006528582.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein diaphanous homolog 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006528582.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TCGGGGCGGC GGCGTCGGGA GCCGGAGGCG GCGGCGGTGG CGGCGAGGAA 
CACGGCGGGG GCCGGAGCAA CAAGCGAGGC GCGGGGAACC GGGCCGCCAA CGAAGAGGAG 
ACGAGAAACA AACCCAAACT GCGTGACCGA ATTACAAGTT TCAGAAAATC TGCCACCAAG 
AGAGAAAAGC CTGTTATTCA ACATTCTATT GATTATCAAA CTGCAGTGGT TGAGATTCCC 
CCAGCTCTGA TAGTACATGA TGACCGATCT TTGATTTTGT CTGAAAAGGA AGTTCTGGAC 
CTCTTTGAGA AAATGATGGA AGATATGAAC CTTAATGAAG AAAAGAAAGC TCCTTTGCGA 
AAGAAGGATT TTAGCATCAA GCGTGAGATG GTTGTCCAGT ATATTTCTGC TACTTCCAAA 
TCTATAGTTG GAAGTAAAGT TCTGGGTGGC CTGAAAAACA GCAAACATGA GTTCACCCTT 
TCTTCACAAG AATATGTTCA TGAATTACGA TCTGGCATCT CAGATGAGAA ACTTCTCAAC 
TGCCTGGAAT CTTTGAGAGT GTCTTTAACC AGCCATCCTG TCAGCTGGGT CAACAACTTT 
GGCTATGAAG GACTTGGAGT CTTACTGGAT GTGCTGGAGA AGCTTCTGGA CAAAAAACAG 
CAAGAAAATA TCGACAAGAA GAATCAGTAC AAAGTCATTC AGTGCCTGAA AGCATTTATG 
AATAATAAGT TTGGACTACA AAGAATTCTA GGAGACGAGA GAAGCCTTTT ACTGTTGGCA 
AGAGCAATTG ACCCGAAACA ACAGAATATG ATGACTGAAA TAGTAAAAAT CCTTTCTGCT 
ATTTGCATTG TTGGGGAAGA AAACATTCTA GACAAACTTT TAGGGGGCAT CACTGCTGCA 
GCTGAACTAA ATAATAGAGA ACGATTTTCA CCAATTGTGG AAGGCTTAGA AAATAATGAA 
GCCTTGCATC TCCAGGTGGC CTGCATGCAG TTCATAAATG CACTTGTCAC CTCTCCATAT 
GATCTTGATT TTCGAATTCA TTTGAGGAAT GAATTCCTTC GTTGTGGATT AAAAGCAATG 
CTCCCTACTC TTAAAGAGAT AGAGAATGAA GGGCTTGATA TTCAGTTGAG AGTGTTTGAA 
GAGAACAAAG AAGATGACCT AAGTGAATTA TCCCACCGTC TCAATGACAT TCGAGCTGAA 
ATGGATGATA TTAATGAAGT CTACCATCTG CTGTATAATA TGCTGAAGGA CACTGCTGCG 
GAACCTTACT TATTATCCAT TCTCCAACAC TTTCTGCTCA TCAGAAATGA TTACTACATC 
AGGCCACAGT ATTATAAAAT CATTGAGGAG TGTGTTTCAC AGATAGTGCT ACACTGCAGT 
GGCATGGACC CAGACTTCAA GTACCGGCAG CGCATAGACT TTGATTTCAC TCATCTCCTA 
GATGCTTGTG TAAATAAGGC AAAAGTTGAA GAAAATGAGC AAAAAGCAAT GGAATTTTCA 
AAGAAGTTCG ATGAAGAATT CACAGCTCGT CAGGAAGCTC AAGCAGAGCT TCAGAAAAGA 
GATGAAAAAA TCAAAGAACT GGAAACAGAA ATCCAGCAGC TTCGAGGACA GGGAGTTCCA 
TCTGCAATTC CAGGTCCACC TCCACCACCT CCACTGCCAG GTGCAGGACC ATGCCCACCA 
CCACCACCTC CTCCTCCTCC ACCACCTCCA CTTCCAGGAG TCGTCCCTCC TCCTCCTCCA 
CCATTACCTG GGATGCCTGG TATTCCACCA CCACCTCCAC CACCTTTGTC TGGAGTACCT 
CCTCCACCAC CACCCCCTGG GGGAGTTTTT CCTCTCCTAT CAGGACCAAT TGAGCTACCA 
TATGGAATGA AGCAGAAAAA ATTATATAAG CCTGACATAC CTATGAAAAG AATCAACTGG 
TCCAAGATTG AGCCTAAAGA ATTATCCGAA AACTGTGTTT GGCTAAAACT TAAAGAAGAG 
AAGTATGAGA ATGCAGATCT ATTTGCAAAA CTGGCCTTGA CATTTCCTTC CCAGATGAAA 
GGTCAAAGGA ATACAGAGGC TGCAGAAGAA AATAGGAGTG GGCCTCCAAA GAAGAAAGTG 
AAGGAATTGA GAATTTTGGA TACCAAAACA GCCCAGAATC TATCTATCTT CCTTGGATCT 
TACCGCATGC CATACGAGGA AATAAAGAAC ATTATTTTGG AGGTTAATGA AGAGATGCTC 
AGTGAGGCCC TAATCCAGAA TCTTGTAAAA TATCTTCCTG ACCAGAACGC ACTCCGAGAA 
CTAGCCCAGC TTAAGAGTGA ATATGATGAT CTCTGTGAGC CTGAGCAGTT TGGTGTTGTG 
ATGAGCACGG TGAAAATGTT ACGTCCTCGT CTCACGAGCA TCCTTTTCAA ACTCACCTTT 
GAAGAACATG TCAACAACAT CAAACCAAGC ATCATAGCAG TAACTCTTGC ATGTGAAGAA 
CTGAAGAAAA GTGAAAGCTT TAAGAGACTC TTAGAGTTGA TTCTTCTGGT TGGAAACTAC 
ATGAACTCAG GCTCCAGGAA TGCCCAGTCT CTAGGTTTTA AGATTAACTT TCTGTGTAAG 
ATCAAAGACA CAAAATCAGC AGATCAAAAA TCCACCCTTT TGCATTTTCT TGCTGAAATC 
TGTGATGAGA AATACCGGGA TATCCTGAAA TTTCCTGATG AGCTGGAGCA TGTGGAAAGC 
GCAGGCAAAG TTTCAGCTCA AATACTCAAG AGCAACCTTG TAGCAATGGA ACAAAGCATT 
CTTCATCTGG AGAAAAACAT CAAGAATTTC CCACCAGCAG AATCGCACCA TGATAAGTTT 
GTGGAAAAGA TGATGAGCTT TACCCAGAAT GCCAGAGAAC AGTATGACAA ACTGTCCACC 
ATGCACAGCA ATATGTTGAA GCTCTATGAG AGTCTGGGAG AATACTTCAT TTTTGACCCG 
AACACAGTAA ACATGGAAGA ATTTTTTGGT GATCTCAACA CCTTCAGAAC CCTATTTTTG 
GAAGCACTGA AAGAAAACCA CAAGAGAAAG GAAATGGAAG AGAAGAGCAG GAGAGCAAAG 
CTTGCAAAAG AGAAAGCGGA ACAAGAGAAG CTGGAACGCC AGAAGAAGAA GAAGCAACTC 
ATTGACATAA ACAAAGAGGG TGATGAGACG GGAGTGATGG ATAATCTTCT GGAAGCCCTA 
CAGTCAGGTG CAGCATTCAG AGACCGCAGA AAGCGAATTC CAAGAAATCC AGATAACAGA 
CGGCCCCCTC TGGAAAGATC TCGCTCTCGC CACAATGGAG CCATGTCATC CAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006528645.1
CDS	312..3608
Translation	MEELGAAASGAGGGGGGGEEHGGGRSNKRGAGNRAANEEETRNKPKLRDRITSFRKSATKREKPVIQHSIDYQTAVVEIPPALIVHDDRSLILSEKEVLDLFEKMMEDMNLNEEKKAPLRKKDFSIKREMVVQYISATSKSIVGSKVLGGLKNSKHEFTLSSQEYVHELRSGISDEKLLNCLESLRVSLTSHPVSWVNNFGYEGLGVLLDVLEKLLDKKQQENIDKKNQYKVIQCLKAFMNNKFGLQRILGDERSLLLLARAIDPKQQNMMTEIVKILSAICIVGEENILDKLLGGITAAAELNNRERFSPIVEGLENNEALHLQVACMQFINALVTSPYDLDFRIHLRNEFLRCGLKAMLPTLKEIENEGLDIQLRVFEENKEDDLSELSHRLNDIRAEMDDINEVYHLLYNMLKDTAAEPYLLSILQHFLLIRNDYYIRPQYYKIIEECVSQIVLHCSGMDPDFKYRQRIDFDFTHLLDACVNKAKVEENEQKAMEFSKKFDEEFTARQEAQAELQKRDEKIKELETEIQQLRGQGVPSAIPGPPPPPPLPGAGPCPPPPPPPPPPPPLPGVVPPPPPPLPGMPGIPPPPPPPLSGVPPPPPPPGGVFPLLSGPIELPYGMKQKKLYKPDIPMKRINWSKIEPKELSENCVWLKLKEEKYENADLFAKLALTFPSQMKGQRNTEAAEENRSGPPKKKVKELRILDTKTAQNLSIFLGSYRMPYEEIKNIILEVNEEMLSEALIQNLVKYLPDQNALRELAQLKSEYDDLCEPEQFGVVMSTVKMLRPRLTSILFKLTFEEHVNNIKPSIIAVTLACEELKKSESFKRLLELILLVGNYMNSGSRNAQSLGFKINFLCKIKDTKSADQKSTLLHFLAEICDEKYRDILKFPDELEHVESAGKVSAQILKSNLVAMEQSILHLEKNIKNFPPAESHHDKFVEKMMSFTQNAREQYDKLSTMHSNMLKLYESLGEYFIFDPNTVNMEEFFGDLNTFRTLFLEALKENHKRKEMEEKSRRAKLAKEKAEQEKLERQKKKKQLIDINKEGDETGVMDNLLEALQSGAAFRDRRKRIPRNPDNRRPPLERSRSRHNGAMSSK

Target ORF information:

RefSeq Version	XM_006528582.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus diaphanous related formin 2 (Diaph2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528582.3

ORF Insert Sequence:

ATGGAGGAGC TCGGGGCGGC GGCGTCGGGA GCCGGAGGCG GCGGCGGTGG CGGCGAGGAA 
CACGGCGGGG GCCGGAGCAA CAAGCGAGGC GCGGGGAACC GGGCCGCCAA CGAAGAGGAG 
ACGAGAAACA AACCCAAACT GCGTGACCGA ATTACAAGTT TCAGAAAATC TGCCACCAAG 
AGAGAAAAGC CTGTTATTCA ACATTCTATT GATTATCAAA CTGCAGTGGT TGAGATTCCC 
CCAGCTCTGA TAGTACATGA TGACCGATCT TTGATTTTGT CTGAAAAGGA AGTTCTGGAC 
CTCTTTGAGA AAATGATGGA AGATATGAAC CTTAATGAAG AAAAGAAAGC TCCTTTGCGA 
AAGAAGGATT TTAGCATCAA GCGTGAGATG GTTGTCCAGT ATATTTCTGC TACTTCCAAA 
TCTATAGTTG GAAGTAAAGT TCTGGGTGGC CTGAAAAACA GCAAACATGA GTTCACCCTT 
TCTTCACAAG AATATGTTCA TGAATTACGA TCTGGCATCT CAGATGAGAA ACTTCTCAAC 
TGCCTGGAAT CTTTGAGAGT GTCTTTAACC AGCCATCCTG TCAGCTGGGT CAACAACTTT 
GGCTATGAAG GACTTGGAGT CTTACTGGAT GTGCTGGAGA AGCTTCTGGA CAAAAAACAG 
CAAGAAAATA TCGACAAGAA GAATCAGTAC AAAGTCATTC AGTGCCTGAA AGCATTTATG 
AATAATAAGT TTGGACTACA AAGAATTCTA GGAGACGAGA GAAGCCTTTT ACTGTTGGCA 
AGAGCAATTG ACCCGAAACA ACAGAATATG ATGACTGAAA TAGTAAAAAT CCTTTCTGCT 
ATTTGCATTG TTGGGGAAGA AAACATTCTA GACAAACTTT TAGGGGGCAT CACTGCTGCA 
GCTGAACTAA ATAATAGAGA ACGATTTTCA CCAATTGTGG AAGGCTTAGA AAATAATGAA 
GCCTTGCATC TCCAGGTGGC CTGCATGCAG TTCATAAATG CACTTGTCAC CTCTCCATAT 
GATCTTGATT TTCGAATTCA TTTGAGGAAT GAATTCCTTC GTTGTGGATT AAAAGCAATG 
CTCCCTACTC TTAAAGAGAT AGAGAATGAA GGGCTTGATA TTCAGTTGAG AGTGTTTGAA 
GAGAACAAAG AAGATGACCT AAGTGAATTA TCCCACCGTC TCAATGACAT TCGAGCTGAA 
ATGGATGATA TTAATGAAGT CTACCATCTG CTGTATAATA TGCTGAAGGA CACTGCTGCG 
GAACCTTACT TATTATCCAT TCTCCAACAC TTTCTGCTCA TCAGAAATGA TTACTACATC 
AGGCCACAGT ATTATAAAAT CATTGAGGAG TGTGTTTCAC AGATAGTGCT ACACTGCAGT 
GGCATGGACC CAGACTTCAA GTACCGGCAG CGCATAGACT TTGATTTCAC TCATCTCCTA 
GATGCTTGTG TAAATAAGGC AAAAGTTGAA GAAAATGAGC AAAAAGCAAT GGAATTTTCA 
AAGAAGTTCG ATGAAGAATT CACAGCTCGT CAGGAAGCTC AAGCAGAGCT TCAGAAAAGA 
GATGAAAAAA TCAAAGAACT GGAAACAGAA ATCCAGCAGC TTCGAGGACA GGGAGTTCCA 
TCTGCAATTC CAGGTCCACC TCCACCACCT CCACTGCCAG GTGCAGGACC ATGCCCACCA 
CCACCACCTC CTCCTCCTCC ACCACCTCCA CTTCCAGGAG TCGTCCCTCC TCCTCCTCCA 
CCATTACCTG GGATGCCTGG TATTCCACCA CCACCTCCAC CACCTTTGTC TGGAGTACCT 
CCTCCACCAC CACCCCCTGG GGGAGTTTTT CCTCTCCTAT CAGGACCAAT TGAGCTACCA 
TATGGAATGA AGCAGAAAAA ATTATATAAG CCTGACATAC CTATGAAAAG AATCAACTGG 
TCCAAGATTG AGCCTAAAGA ATTATCCGAA AACTGTGTTT GGCTAAAACT TAAAGAAGAG 
AAGTATGAGA ATGCAGATCT ATTTGCAAAA CTGGCCTTGA CATTTCCTTC CCAGATGAAA 
GGTCAAAGGA ATACAGAGGC TGCAGAAGAA AATAGGAGTG GGCCTCCAAA GAAGAAAGTG 
AAGGAATTGA GAATTTTGGA TACCAAAACA GCCCAGAATC TATCTATCTT CCTTGGATCT 
TACCGCATGC CATACGAGGA AATAAAGAAC ATTATTTTGG AGGTTAATGA AGAGATGCTC 
AGTGAGGCCC TAATCCAGAA TCTTGTAAAA TATCTTCCTG ACCAGAACGC ACTCCGAGAA 
CTAGCCCAGC TTAAGAGTGA ATATGATGAT CTCTGTGAGC CTGAGCAGTT TGGTGTTGTG 
ATGAGCACGG TGAAAATGTT ACGTCCTCGT CTCACGAGCA TCCTTTTCAA ACTCACCTTT 
GAAGAACATG TCAACAACAT CAAACCAAGC ATCATAGCAG TAACTCTTGC ATGTGAAGAA 
CTGAAGAAAA GTGAAAGCTT TAAGAGACTC TTAGAGTTGA TTCTTCTGGT TGGAAACTAC 
ATGAACTCAG GCTCCAGGAA TGCCCAGTCT CTAGGTTTTA AGATTAACTT TCTGTGTAAG 
ATCAAAGACA CAAAATCAGC AGATCAAAAA TCCACCCTTT TGCATTTTCT TGCTGAAATC 
TGTGATGAGA AATACCGGGA TATCCTGAAA TTTCCTGATG AGCTGGAGCA TGTGGAAAGC 
GCAGGCAAAG TTTCAGCTCA AATACTCAAG AGCAACCTTG TAGCAATGGA ACAAAGCATT 
CTTCATCTGG AGAAAAACAT CAAGAATTTC CCACCAGCAG AATCGCACCA TGATAAGTTT 
GTGGAAAAGA TGATGAGCTT TACCCAGAAT GCCAGAGAAC AGTATGACAA ACTGTCCACC 
ATGCACAGCA ATATGTTGAA GCTCTATGAG AGTCTGGGAG AATACTTCAT TTTTGACCCG 
AACACAGTAA ACATGGAAGA ATTTTTTGGT GATCTCAACA CCTTCAGAAC CCTATTTTTG 
GAAGCACTGA AAGAAAACCA CAAGAGAAAG GAAATGGAAG AGAAGAGCAG GAGAGCAAAG 
CTTGCAAAAG AGAAAGCGGA ACAAGAGAAG CTGGAACGCC AGAAGAAGAA GAAGCAACTC 
ATTGACATAA ACAAAGAGGG TGATGAGACG GGAGTGATGG ATAATCTTCT GGAAGCCCTA 
CAGTCAGGTG CAGCATTCAG AGACCGCAGA AAGCGAATTC CAAGAAATCC AGATAACAGA 
CGGCCCCCTC TGGAAAGATC TCGCTCTCGC CACAATGGAG CCATGTCATC CAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

mDia and ROCK Mediate Actin-Dependent Presynaptic Remodeling Regulating Synaptic Efficacy and Anxiety.
Cell reports17(9)2405-2417(2016 11)
Deguchi Y,Harada M,Shinohara R,Lazarus M,Cherasse Y,Urade Y,Yamada D,Sekiguchi M,Watanabe D,Furuyashiki T,Narumiya S

Expression of multiple formins in adult tissues and during developmental stages of mouse brain.
Gene expression patterns : GEP19(1-2)52-9(2015 Sep-Nov)
Dutta P,Maiti S

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Diaph2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Mus musculus (house mouse)	Diap2	NP_766081.1
Rattus norvegicus (Norway rat)	Diaph2	XP_228441.7