MARCHF1 cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following MARCHF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MARCHF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu11650 NM_001166373.1
Latest version!
Homo sapiens membrane associated ring-CH-type finger 1 (MARCHF1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$99.50-$139.30
$199.00
OHu11650 XM_017008334.1
Latest version!
Homo sapiens membrane associated ring-CH-type finger 1 (MARCHF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$99.50-$139.30
$199.00
OHu11650 XM_011532056.1
Latest version!
Homo sapiens membrane-associated ring finger (C3HC4) 1, E3 ubiquitin protein ligase (MARCH1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$99.50-$139.30
$199.00
OHu10994 NM_017923.4
Latest version!
Homo sapiens membrane associated ring-CH-type finger 1 (MARCHF1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
OHu68908 XM_011532055.3
Latest version!
Homo sapiens membrane associated ring-CH-type finger 1 (MARCHF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu95249 XM_017008335.1
Latest version!
Homo sapiens membrane associated ring-CH-type finger 1 (MARCHF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
OHu95249 XM_017008336.1
Latest version!
Homo sapiens membrane associated ring-CH-type finger 1 (MARCHF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
View Old Accession Versions >>
OHu10994 NM_017923.3 Homo sapiens membrane associated ring-CH-type finger 1 (MARCH1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
OHu68908 XM_011532055.2 Homo sapiens membrane associated ring-CH-type finger 1 (MARCH1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu11650
    Clone ID Related Accession (Same CDS sequence) NM_001166373.1 , XM_011532056.1 , XM_017008334.1
    Accession Version NM_001166373.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-11
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase MARCH1 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK289711.1, AL713759.1 and AC093788.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK289711.1, SRR1803612.19868.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGCTGGGCT GGTGTGAAGC GATAGCCCGT AACCCTCACA GAATTCCAAA CAACACGCGA 
    ACACCCGAGA TCTCAGGGGA TTTGGCTGAC GCCTCACAAA CCTCCACATT GAATGAAAAA
    TCCCCAGGGC GATCTGCAAG TCGATCAAGT AACATTTCAA AAGCAAGCAG CCCAACAACA
    GGGACAGCTC CCAGGAGCCA GTCAAGGTTG TCTGTCTGTC CATCCACTCA GGACATCTGC
    AGAATCTGTC ACTGCGAAGG GGATGAAGAG AGCCCCCTCA TCACACCCTG TCGCTGCACT
    GGGACACTGC GCTTTGTCCA CCAGTCCTGC CTCCACCAGT GGATAAAGAG CTCAGATACA
    CGCTGCTGTG AGCTCTGCAA GTATGACTTC ATAATGGAGA CCAAGCTCAA ACCCCTCCGG
    AAGTGGGAGA AACTACAGAT GACCACAAGT GAAAGGAGGA AAATATTCTG CTCTGTCACA
    TTCCACGTAA TCGCGATCAC CTGTGTGGTT TGGTCTTTGT ATGTATTGAT AGACCGGACA
    GCGGAGGAAA TCAAGCAAGG CAATGACAAT GGTGTCCTTG AATGGCCATT TTGGACAAAA
    CTGGTTGTGG TAGCCATTGG CTTCACAGGA GGTCTTGTCT TCATGTACGT ACAGTGTAAA
    GTCTATGTTC AGTTGTGGCG CAGGCTGAAG GCCTACAACC GTGTGATCTT TGTACAAAAT
    TGCCCAGACA CTGCCAAAAA ACTGGAGAAG AACTTCTCAT GTAATGTAAA CACAGACATC
    AAAGATGCTG TGGTAGTGCC TGTACCACAA ACAGGTGCAA ATTCACTGCC ATCTGCAGAG
    GGTGGCCCCC CTGAAGTTGT ATCAGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001159845.1
    CDS183..1052
    Translation

    Target ORF information:

    RefSeq Version NM_001166373.1
    Organism Homo sapiens(human)
    Definition Homo sapiens membrane associated ring-CH-type finger 1 (MARCHF1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001166373.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGCTGGGCT GGTGTGAAGC GATAGCCCGT AACCCTCACA GAATTCCAAA CAACACGCGA 
    ACACCCGAGA TCTCAGGGGA TTTGGCTGAC GCCTCACAAA CCTCCACATT GAATGAAAAA
    TCCCCAGGGC GATCTGCAAG TCGATCAAGT AACATTTCAA AAGCAAGCAG CCCAACAACA
    GGGACAGCTC CCAGGAGCCA GTCAAGGTTG TCTGTCTGTC CATCCACTCA GGACATCTGC
    AGAATCTGTC ACTGCGAAGG GGATGAAGAG AGCCCCCTCA TCACACCCTG TCGCTGCACT
    GGGACACTGC GCTTTGTCCA CCAGTCCTGC CTCCACCAGT GGATAAAGAG CTCAGATACA
    CGCTGCTGTG AGCTCTGCAA GTATGACTTC ATAATGGAGA CCAAGCTCAA ACCCCTCCGG
    AAGTGGGAGA AACTACAGAT GACCACAAGT GAAAGGAGGA AAATATTCTG CTCTGTCACA
    TTCCACGTAA TCGCGATCAC CTGTGTGGTT TGGTCTTTGT ATGTATTGAT AGACCGGACA
    GCGGAGGAAA TCAAGCAAGG CAATGACAAT GGTGTCCTTG AATGGCCATT TTGGACAAAA
    CTGGTTGTGG TAGCCATTGG CTTCACAGGA GGTCTTGTCT TCATGTACGT ACAGTGTAAA
    GTCTATGTTC AGTTGTGGCG CAGGCTGAAG GCCTACAACC GTGTGATCTT TGTACAAAAT
    TGCCCAGACA CTGCCAAAAA ACTGGAGAAG AACTTCTCAT GTAATGTAAA CACAGACATC
    AAAGATGCTG TGGTAGTGCC TGTACCACAA ACAGGTGCAA ATTCACTGCC ATCTGCAGAG
    GGTGGCCCCC CTGAAGTTGT ATCAGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu11650
    Clone ID Related Accession (Same CDS sequence) NM_001166373.1 , XM_011532056.1 , XM_017008334.1
    Accession Version XM_011532056.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product E3 ubiquitin-protein ligase MARCH1 isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGCTGGGCT GGTGTGAAGC GATAGCCCGT AACCCTCACA GAATTCCAAA CAACACGCGA 
    ACACCCGAGA TCTCAGGGGA TTTGGCTGAC GCCTCACAAA CCTCCACATT GAATGAAAAA
    TCCCCAGGGC GATCTGCAAG TCGATCAAGT AACATTTCAA AAGCAAGCAG CCCAACAACA
    GGGACAGCTC CCAGGAGCCA GTCAAGGTTG TCTGTCTGTC CATCCACTCA GGACATCTGC
    AGAATCTGTC ACTGCGAAGG GGATGAAGAG AGCCCCCTCA TCACACCCTG TCGCTGCACT
    GGGACACTGC GCTTTGTCCA CCAGTCCTGC CTCCACCAGT GGATAAAGAG CTCAGATACA
    CGCTGCTGTG AGCTCTGCAA GTATGACTTC ATAATGGAGA CCAAGCTCAA ACCCCTCCGG
    AAGTGGGAGA AACTACAGAT GACCACAAGT GAAAGGAGGA AAATATTCTG CTCTGTCACA
    TTCCACGTAA TCGCGATCAC CTGTGTGGTT TGGTCTTTGT ATGTATTGAT AGACCGGACA
    GCGGAGGAAA TCAAGCAAGG CAATGACAAT GGTGTCCTTG AATGGCCATT TTGGACAAAA
    CTGGTTGTGG TAGCCATTGG CTTCACAGGA GGTCTTGTCT TCATGTACGT ACAGTGTAAA
    GTCTATGTTC AGTTGTGGCG CAGGCTGAAG GCCTACAACC GTGTGATCTT TGTACAAAAT
    TGCCCAGACA CTGCCAAAAA ACTGGAGAAG AACTTCTCAT GTAATGTAAA CACAGACATC
    AAAGATGCTG TGGTAGTGCC TGTACCACAA ACAGGTGCAA ATTCACTGCC ATCTGCAGAG
    GGTGGCCCCC CTGAAGTTGT ATCAGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011530358.1
    Translation

    Target ORF information:

    RefSeq Version XM_011532056.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens membrane-associated ring finger (C3HC4) 1, E3 ubiquitin protein ligase (MARCH1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011532056.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGCTGGGCT GGTGTGAAGC GATAGCCCGT AACCCTCACA GAATTCCAAA CAACACGCGA 
    ACACCCGAGA TCTCAGGGGA TTTGGCTGAC GCCTCACAAA CCTCCACATT GAATGAAAAA
    TCCCCAGGGC GATCTGCAAG TCGATCAAGT AACATTTCAA AAGCAAGCAG CCCAACAACA
    GGGACAGCTC CCAGGAGCCA GTCAAGGTTG TCTGTCTGTC CATCCACTCA GGACATCTGC
    AGAATCTGTC ACTGCGAAGG GGATGAAGAG AGCCCCCTCA TCACACCCTG TCGCTGCACT
    GGGACACTGC GCTTTGTCCA CCAGTCCTGC CTCCACCAGT GGATAAAGAG CTCAGATACA
    CGCTGCTGTG AGCTCTGCAA GTATGACTTC ATAATGGAGA CCAAGCTCAA ACCCCTCCGG
    AAGTGGGAGA AACTACAGAT GACCACAAGT GAAAGGAGGA AAATATTCTG CTCTGTCACA
    TTCCACGTAA TCGCGATCAC CTGTGTGGTT TGGTCTTTGT ATGTATTGAT AGACCGGACA
    GCGGAGGAAA TCAAGCAAGG CAATGACAAT GGTGTCCTTG AATGGCCATT TTGGACAAAA
    CTGGTTGTGG TAGCCATTGG CTTCACAGGA GGTCTTGTCT TCATGTACGT ACAGTGTAAA
    GTCTATGTTC AGTTGTGGCG CAGGCTGAAG GCCTACAACC GTGTGATCTT TGTACAAAAT
    TGCCCAGACA CTGCCAAAAA ACTGGAGAAG AACTTCTCAT GTAATGTAAA CACAGACATC
    AAAGATGCTG TGGTAGTGCC TGTACCACAA ACAGGTGCAA ATTCACTGCC ATCTGCAGAG
    GGTGGCCCCC CTGAAGTTGT ATCAGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu11650
    Clone ID Related Accession (Same CDS sequence) NM_001166373.1 , XM_011532056.1 , XM_017008334.1
    Accession Version XM_017008334.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase MARCH1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGCTGGGCT GGTGTGAAGC GATAGCCCGT AACCCTCACA GAATTCCAAA CAACACGCGA 
    ACACCCGAGA TCTCAGGGGA TTTGGCTGAC GCCTCACAAA CCTCCACATT GAATGAAAAA
    TCCCCAGGGC GATCTGCAAG TCGATCAAGT AACATTTCAA AAGCAAGCAG CCCAACAACA
    GGGACAGCTC CCAGGAGCCA GTCAAGGTTG TCTGTCTGTC CATCCACTCA GGACATCTGC
    AGAATCTGTC ACTGCGAAGG GGATGAAGAG AGCCCCCTCA TCACACCCTG TCGCTGCACT
    GGGACACTGC GCTTTGTCCA CCAGTCCTGC CTCCACCAGT GGATAAAGAG CTCAGATACA
    CGCTGCTGTG AGCTCTGCAA GTATGACTTC ATAATGGAGA CCAAGCTCAA ACCCCTCCGG
    AAGTGGGAGA AACTACAGAT GACCACAAGT GAAAGGAGGA AAATATTCTG CTCTGTCACA
    TTCCACGTAA TCGCGATCAC CTGTGTGGTT TGGTCTTTGT ATGTATTGAT AGACCGGACA
    GCGGAGGAAA TCAAGCAAGG CAATGACAAT GGTGTCCTTG AATGGCCATT TTGGACAAAA
    CTGGTTGTGG TAGCCATTGG CTTCACAGGA GGTCTTGTCT TCATGTACGT ACAGTGTAAA
    GTCTATGTTC AGTTGTGGCG CAGGCTGAAG GCCTACAACC GTGTGATCTT TGTACAAAAT
    TGCCCAGACA CTGCCAAAAA ACTGGAGAAG AACTTCTCAT GTAATGTAAA CACAGACATC
    AAAGATGCTG TGGTAGTGCC TGTACCACAA ACAGGTGCAA ATTCACTGCC ATCTGCAGAG
    GGTGGCCCCC CTGAAGTTGT ATCAGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016863823.1
    CDS110..979
    Translation

    Target ORF information:

    RefSeq Version XM_017008334.1
    Organism Homo sapiens(human)
    Definition Homo sapiens membrane associated ring-CH-type finger 1 (MARCHF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008334.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGCTGGGCT GGTGTGAAGC GATAGCCCGT AACCCTCACA GAATTCCAAA CAACACGCGA 
    ACACCCGAGA TCTCAGGGGA TTTGGCTGAC GCCTCACAAA CCTCCACATT GAATGAAAAA
    TCCCCAGGGC GATCTGCAAG TCGATCAAGT AACATTTCAA AAGCAAGCAG CCCAACAACA
    GGGACAGCTC CCAGGAGCCA GTCAAGGTTG TCTGTCTGTC CATCCACTCA GGACATCTGC
    AGAATCTGTC ACTGCGAAGG GGATGAAGAG AGCCCCCTCA TCACACCCTG TCGCTGCACT
    GGGACACTGC GCTTTGTCCA CCAGTCCTGC CTCCACCAGT GGATAAAGAG CTCAGATACA
    CGCTGCTGTG AGCTCTGCAA GTATGACTTC ATAATGGAGA CCAAGCTCAA ACCCCTCCGG
    AAGTGGGAGA AACTACAGAT GACCACAAGT GAAAGGAGGA AAATATTCTG CTCTGTCACA
    TTCCACGTAA TCGCGATCAC CTGTGTGGTT TGGTCTTTGT ATGTATTGAT AGACCGGACA
    GCGGAGGAAA TCAAGCAAGG CAATGACAAT GGTGTCCTTG AATGGCCATT TTGGACAAAA
    CTGGTTGTGG TAGCCATTGG CTTCACAGGA GGTCTTGTCT TCATGTACGT ACAGTGTAAA
    GTCTATGTTC AGTTGTGGCG CAGGCTGAAG GCCTACAACC GTGTGATCTT TGTACAAAAT
    TGCCCAGACA CTGCCAAAAA ACTGGAGAAG AACTTCTCAT GTAATGTAAA CACAGACATC
    AAAGATGCTG TGGTAGTGCC TGTACCACAA ACAGGTGCAA ATTCACTGCC ATCTGCAGAG
    GGTGGCCCCC CTGAAGTTGT ATCAGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu10994
    Clone ID Related Accession (Same CDS sequence) NM_017923.4 , NM_017923.3
    Accession Version NM_017923.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 819bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-11
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase MARCH1 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AW118953.1, AC098867.3, AC108154.4 and AC093788.3. On Jun 2, 2019 this sequence version replaced NM_017923.3. Transcript Variant: This variant (2) lacks multiple exons at its 5' end and has a novel segment at its 5' end, compared to variant 1. These differences result in a protein (isoform 2) with a shorter and distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DRR138524.64176.1, SRR1660803.12839.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01820689 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGACCAGCA GCCACGTTTG TTGTAATTTT TTAAATATGT GGAAAAAAAG CAAGATATCA 
    ACCATGTATT ACCTTAACCA AGATGCCAAA TTATCTAACT TGTTTCTCCA GGCAAGCAGC
    CCAACAACAG GGACAGCTCC CAGGAGCCAG TCAAGGTTGT CTGTCTGTCC ATCCACTCAG
    GACATCTGCA GAATCTGTCA CTGCGAAGGG GATGAAGAGA GCCCCCTCAT CACACCCTGT
    CGCTGCACTG GGACACTGCG CTTTGTCCAC CAGTCCTGCC TCCACCAGTG GATAAAGAGC
    TCAGATACAC GCTGCTGTGA GCTCTGCAAG TATGACTTCA TAATGGAGAC CAAGCTCAAA
    CCCCTCCGGA AGTGGGAGAA ACTACAGATG ACCACAAGTG AAAGGAGGAA AATATTCTGC
    TCTGTCACAT TCCACGTAAT CGCGATCACC TGTGTGGTTT GGTCTTTGTA TGTATTGATA
    GACCGGACAG CGGAGGAAAT CAAGCAAGGC AATGACAATG GTGTCCTTGA ATGGCCATTT
    TGGACAAAAC TGGTTGTGGT AGCCATTGGC TTCACAGGAG GTCTTGTCTT CATGTACGTA
    CAGTGTAAAG TCTATGTTCA GTTGTGGCGC AGGCTGAAGG CCTACAACCG TGTGATCTTT
    GTACAAAATT GCCCAGACAC TGCCAAAAAA CTGGAGAAGA ACTTCTCATG TAATGTAAAC
    ACAGACATCA AAGATGCTGT GGTAGTGCCT GTACCACAAA CAGGTGCAAA TTCACTGCCA
    TCTGCAGAGG GTGGCCCCCC TGAAGTTGTA TCAGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_060393.1
    CDS31..849
    Translation

    Target ORF information:

    RefSeq Version NM_017923.4
    Organism Homo sapiens(human)
    Definition Homo sapiens membrane associated ring-CH-type finger 1 (MARCHF1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_017923.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGACCAGCA GCCACGTTTG TTGTAATTTT TTAAATATGT GGAAAAAAAG CAAGATATCA 
    ACCATGTATT ACCTTAACCA AGATGCCAAA TTATCTAACT TGTTTCTCCA GGCAAGCAGC
    CCAACAACAG GGACAGCTCC CAGGAGCCAG TCAAGGTTGT CTGTCTGTCC ATCCACTCAG
    GACATCTGCA GAATCTGTCA CTGCGAAGGG GATGAAGAGA GCCCCCTCAT CACACCCTGT
    CGCTGCACTG GGACACTGCG CTTTGTCCAC CAGTCCTGCC TCCACCAGTG GATAAAGAGC
    TCAGATACAC GCTGCTGTGA GCTCTGCAAG TATGACTTCA TAATGGAGAC CAAGCTCAAA
    CCCCTCCGGA AGTGGGAGAA ACTACAGATG ACCACAAGTG AAAGGAGGAA AATATTCTGC
    TCTGTCACAT TCCACGTAAT CGCGATCACC TGTGTGGTTT GGTCTTTGTA TGTATTGATA
    GACCGGACAG CGGAGGAAAT CAAGCAAGGC AATGACAATG GTGTCCTTGA ATGGCCATTT
    TGGACAAAAC TGGTTGTGGT AGCCATTGGC TTCACAGGAG GTCTTGTCTT CATGTACGTA
    CAGTGTAAAG TCTATGTTCA GTTGTGGCGC AGGCTGAAGG CCTACAACCG TGTGATCTTT
    GTACAAAATT GCCCAGACAC TGCCAAAAAA CTGGAGAAGA ACTTCTCATG TAATGTAAAC
    ACAGACATCA AAGATGCTGT GGTAGTGCCT GTACCACAAA CAGGTGCAAA TTCACTGCCA
    TCTGCAGAGG GTGGCCCCCC TGAAGTTGTA TCAGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu10994
    Clone ID Related Accession (Same CDS sequence) NM_017923.4 , NM_017923.3
    Accession Version NM_017923.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 819bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-21
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase MARCH1 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AW118953.1, AC098867.3, AC108154.4 and AC093788.3. On Oct 21, 2009 this sequence version replaced NM_017923.2. Transcript Variant: This variant (2) lacks multiple exons at its 5' end and has a novel segment at its 5' end, compared to variant 1. These differences result in a protein (isoform 2) with a shorter and distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.12839.1, SRR1660809.174285.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01820689 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGACCAGCA GCCACGTTTG TTGTAATTTT TTAAATATGT GGAAAAAAAG CAAGATATCA 
    ACCATGTATT ACCTTAACCA AGATGCCAAA TTATCTAACT TGTTTCTCCA GGCAAGCAGC
    CCAACAACAG GGACAGCTCC CAGGAGCCAG TCAAGGTTGT CTGTCTGTCC ATCCACTCAG
    GACATCTGCA GAATCTGTCA CTGCGAAGGG GATGAAGAGA GCCCCCTCAT CACACCCTGT
    CGCTGCACTG GGACACTGCG CTTTGTCCAC CAGTCCTGCC TCCACCAGTG GATAAAGAGC
    TCAGATACAC GCTGCTGTGA GCTCTGCAAG TATGACTTCA TAATGGAGAC CAAGCTCAAA
    CCCCTCCGGA AGTGGGAGAA ACTACAGATG ACCACAAGTG AAAGGAGGAA AATATTCTGC
    TCTGTCACAT TCCACGTAAT CGCGATCACC TGTGTGGTTT GGTCTTTGTA TGTATTGATA
    GACCGGACAG CGGAGGAAAT CAAGCAAGGC AATGACAATG GTGTCCTTGA ATGGCCATTT
    TGGACAAAAC TGGTTGTGGT AGCCATTGGC TTCACAGGAG GTCTTGTCTT CATGTACGTA
    CAGTGTAAAG TCTATGTTCA GTTGTGGCGC AGGCTGAAGG CCTACAACCG TGTGATCTTT
    GTACAAAATT GCCCAGACAC TGCCAAAAAA CTGGAGAAGA ACTTCTCATG TAATGTAAAC
    ACAGACATCA AAGATGCTGT GGTAGTGCCT GTACCACAAA CAGGTGCAAA TTCACTGCCA
    TCTGCAGAGG GTGGCCCCCC TGAAGTTGTA TCAGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_060393.1
    CDS121..939
    Translation

    Target ORF information:

    RefSeq Version NM_017923.3
    Organism Homo sapiens(human)
    Definition Homo sapiens membrane associated ring-CH-type finger 1 (MARCH1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_017923.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGACCAGCA GCCACGTTTG TTGTAATTTT TTAAATATGT GGAAAAAAAG CAAGATATCA 
    ACCATGTATT ACCTTAACCA AGATGCCAAA TTATCTAACT TGTTTCTCCA GGCAAGCAGC
    CCAACAACAG GGACAGCTCC CAGGAGCCAG TCAAGGTTGT CTGTCTGTCC ATCCACTCAG
    GACATCTGCA GAATCTGTCA CTGCGAAGGG GATGAAGAGA GCCCCCTCAT CACACCCTGT
    CGCTGCACTG GGACACTGCG CTTTGTCCAC CAGTCCTGCC TCCACCAGTG GATAAAGAGC
    TCAGATACAC GCTGCTGTGA GCTCTGCAAG TATGACTTCA TAATGGAGAC CAAGCTCAAA
    CCCCTCCGGA AGTGGGAGAA ACTACAGATG ACCACAAGTG AAAGGAGGAA AATATTCTGC
    TCTGTCACAT TCCACGTAAT CGCGATCACC TGTGTGGTTT GGTCTTTGTA TGTATTGATA
    GACCGGACAG CGGAGGAAAT CAAGCAAGGC AATGACAATG GTGTCCTTGA ATGGCCATTT
    TGGACAAAAC TGGTTGTGGT AGCCATTGGC TTCACAGGAG GTCTTGTCTT CATGTACGTA
    CAGTGTAAAG TCTATGTTCA GTTGTGGCGC AGGCTGAAGG CCTACAACCG TGTGATCTTT
    GTACAAAATT GCCCAGACAC TGCCAAAAAA CTGGAGAAGA ACTTCTCATG TAATGTAAAC
    ACAGACATCA AAGATGCTGT GGTAGTGCCT GTACCACAAA CAGGTGCAAA TTCACTGCCA
    TCTGCAGAGG GTGGCCCCCC TGAAGTTGTA TCAGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu68908
    Clone ID Related Accession (Same CDS sequence) XM_011532055.2 , XM_011532055.3
    Accession Version XM_011532055.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1638bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase MARCH1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011532055.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGCTGGGCT GGTGTGAAGC GATAGCCCGT AACCCTCACA GAATTCCAAA CAACACGCGA 
    ACACCCGAGA TCTCAGGGGA TTTGGCTGAC GCCTCACAAA CCTCCACATT GAATGAAAAA
    TCCCCAGGGC GATCTGCAAG TCGATCAAGT AACATTTCAA AAGCAAGCAG CCCAACAACA
    GGGACAGCTC CCAGGAGCCA GTCAAGGTTG TCTGTCTGTC CATCCACTCA GGACATCTGC
    AGATCTGCCA TCTTGCATGA CATGTCAGAA GAGTCATTTG AGTATTGCAC CCCTGTCATG
    GTGCTGAGCC CTGCTAGGAA GGAGTCTGGT AAGAAATCAG TAATACAAAG ACCTAGGAGG
    AGGAGAAAAG CCTCTGAGAG ATATGAGCAT GCTGCAGAAG AACAAATAAG AGGGAGAAAA
    AATGACTTTC ACCTTCAAAT CTCAAGCCCC AGGTGGAGGG AGCTTTACAC AGATTCTTCA
    GATTCATCTT CCACAGATGA GAGTCATTGG ATTCAGGCAA AAAGAAGAGC CCAGGTTAAA
    TTCAGACTGT CAAGAAGAAG GAGGAGAAAT AATAATAAGC CGTGTGAAAA CTTAGCTGGG
    TCTTCCACTC CTAACGGAAT TGAGCTCGTT GATCTGGGAT CCAAAGGTAA AGAGCAACAA
    GAGCTGATTG AATGTGAGAG TTGCTCTTTA AATCTCCACA GAGGAAAACA TACAAGGTAC
    CAAGAATGCA ACCCTTCTCT GACTCAAGGG TCCTCTGAAA TTAAGAGATC CTCAAGGAAT
    CATGAGAAGA GCAAAGGCAG ATATCACCAC AGAGATCCTC AGCTCTTGCA AAGTCTTAGG
    AAAAATGAAA TAATGAAGAA GACATTTTCA GAAACAGATT CCAGCACTGA AATACTGGGA
    GTTCCAGAAG GCAGCAAGGA CATGAATGAT GCAGGGCTCC AGGTGAATAA CCCTGTTCAG
    AAGCCTCCTG CCACCTATGA CGATGGGTCT GATAATTTAG AAGTATGCAG AATCTGTCAC
    TGCGAAGGGG ATGAAGAGAG CCCCCTCATC ACACCCTGTC GCTGCACTGG GACACTGCGC
    TTTGTCCACC AGTCCTGCCT CCACCAGTGG ATAAAGAGCT CAGATACACG CTGCTGTGAG
    CTCTGCAAGT ATGACTTCAT AATGGAGACC AAGCTCAAAC CCCTCCGGAA GTGGGAGAAA
    CTACAGATGA CCACAAGTGA AAGGAGGAAA ATATTCTGCT CTGTCACATT CCACGTAATC
    GCGATCACCT GTGTGGTTTG GTCTTTGTAT GTATTGATAG ACCGGACAGC GGAGGAAATC
    AAGCAAGGCA ATGACAATGG TGTCCTTGAA TGGCCATTTT GGACAAAACT GGTTGTGGTA
    GCCATTGGCT TCACAGGAGG TCTTGTCTTC ATGTACGTAC AGTGTAAAGT CTATGTTCAG
    TTGTGGCGCA GGCTGAAGGC CTACAACCGT GTGATCTTTG TACAAAATTG CCCAGACACT
    GCCAAAAAAC TGGAGAAGAA CTTCTCATGT AATGTAAACA CAGACATCAA AGATGCTGTG
    GTAGTGCCTG TACCACAAAC AGGTGCAAAT TCACTGCCAT CTGCAGAGGG TGGCCCCCCT
    GAAGTTGTAT CAGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011530357.1
    CDS120..1757
    Translation

    Target ORF information:

    RefSeq Version XM_011532055.2
    Organism Homo sapiens(human)
    Definition Homo sapiens membrane associated ring-CH-type finger 1 (MARCH1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011532055.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGCTGGGCT GGTGTGAAGC GATAGCCCGT AACCCTCACA GAATTCCAAA CAACACGCGA 
    ACACCCGAGA TCTCAGGGGA TTTGGCTGAC GCCTCACAAA CCTCCACATT GAATGAAAAA
    TCCCCAGGGC GATCTGCAAG TCGATCAAGT AACATTTCAA AAGCAAGCAG CCCAACAACA
    GGGACAGCTC CCAGGAGCCA GTCAAGGTTG TCTGTCTGTC CATCCACTCA GGACATCTGC
    AGATCTGCCA TCTTGCATGA CATGTCAGAA GAGTCATTTG AGTATTGCAC CCCTGTCATG
    GTGCTGAGCC CTGCTAGGAA GGAGTCTGGT AAGAAATCAG TAATACAAAG ACCTAGGAGG
    AGGAGAAAAG CCTCTGAGAG ATATGAGCAT GCTGCAGAAG AACAAATAAG AGGGAGAAAA
    AATGACTTTC ACCTTCAAAT CTCAAGCCCC AGGTGGAGGG AGCTTTACAC AGATTCTTCA
    GATTCATCTT CCACAGATGA GAGTCATTGG ATTCAGGCAA AAAGAAGAGC CCAGGTTAAA
    TTCAGACTGT CAAGAAGAAG GAGGAGAAAT AATAATAAGC CGTGTGAAAA CTTAGCTGGG
    TCTTCCACTC CTAACGGAAT TGAGCTCGTT GATCTGGGAT CCAAAGGTAA AGAGCAACAA
    GAGCTGATTG AATGTGAGAG TTGCTCTTTA AATCTCCACA GAGGAAAACA TACAAGGTAC
    CAAGAATGCA ACCCTTCTCT GACTCAAGGG TCCTCTGAAA TTAAGAGATC CTCAAGGAAT
    CATGAGAAGA GCAAAGGCAG ATATCACCAC AGAGATCCTC AGCTCTTGCA AAGTCTTAGG
    AAAAATGAAA TAATGAAGAA GACATTTTCA GAAACAGATT CCAGCACTGA AATACTGGGA
    GTTCCAGAAG GCAGCAAGGA CATGAATGAT GCAGGGCTCC AGGTGAATAA CCCTGTTCAG
    AAGCCTCCTG CCACCTATGA CGATGGGTCT GATAATTTAG AAGTATGCAG AATCTGTCAC
    TGCGAAGGGG ATGAAGAGAG CCCCCTCATC ACACCCTGTC GCTGCACTGG GACACTGCGC
    TTTGTCCACC AGTCCTGCCT CCACCAGTGG ATAAAGAGCT CAGATACACG CTGCTGTGAG
    CTCTGCAAGT ATGACTTCAT AATGGAGACC AAGCTCAAAC CCCTCCGGAA GTGGGAGAAA
    CTACAGATGA CCACAAGTGA AAGGAGGAAA ATATTCTGCT CTGTCACATT CCACGTAATC
    GCGATCACCT GTGTGGTTTG GTCTTTGTAT GTATTGATAG ACCGGACAGC GGAGGAAATC
    AAGCAAGGCA ATGACAATGG TGTCCTTGAA TGGCCATTTT GGACAAAACT GGTTGTGGTA
    GCCATTGGCT TCACAGGAGG TCTTGTCTTC ATGTACGTAC AGTGTAAAGT CTATGTTCAG
    TTGTGGCGCA GGCTGAAGGC CTACAACCGT GTGATCTTTG TACAAAATTG CCCAGACACT
    GCCAAAAAAC TGGAGAAGAA CTTCTCATGT AATGTAAACA CAGACATCAA AGATGCTGTG
    GTAGTGCCTG TACCACAAAC AGGTGCAAAT TCACTGCCAT CTGCAGAGGG TGGCCCCCCT
    GAAGTTGTAT CAGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu68908
    Clone ID Related Accession (Same CDS sequence) XM_011532055.2 , XM_011532055.3
    Accession Version XM_011532055.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1638bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase MARCH1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011532055.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGCTGGGCT GGTGTGAAGC GATAGCCCGT AACCCTCACA GAATTCCAAA CAACACGCGA 
    ACACCCGAGA TCTCAGGGGA TTTGGCTGAC GCCTCACAAA CCTCCACATT GAATGAAAAA
    TCCCCAGGGC GATCTGCAAG TCGATCAAGT AACATTTCAA AAGCAAGCAG CCCAACAACA
    GGGACAGCTC CCAGGAGCCA GTCAAGGTTG TCTGTCTGTC CATCCACTCA GGACATCTGC
    AGATCTGCCA TCTTGCATGA CATGTCAGAA GAGTCATTTG AGTATTGCAC CCCTGTCATG
    GTGCTGAGCC CTGCTAGGAA GGAGTCTGGT AAGAAATCAG TAATACAAAG ACCTAGGAGG
    AGGAGAAAAG CCTCTGAGAG ATATGAGCAT GCTGCAGAAG AACAAATAAG AGGGAGAAAA
    AATGACTTTC ACCTTCAAAT CTCAAGCCCC AGGTGGAGGG AGCTTTACAC AGATTCTTCA
    GATTCATCTT CCACAGATGA GAGTCATTGG ATTCAGGCAA AAAGAAGAGC CCAGGTTAAA
    TTCAGACTGT CAAGAAGAAG GAGGAGAAAT AATAATAAGC CGTGTGAAAA CTTAGCTGGG
    TCTTCCACTC CTAACGGAAT TGAGCTCGTT GATCTGGGAT CCAAAGGTAA AGAGCAACAA
    GAGCTGATTG AATGTGAGAG TTGCTCTTTA AATCTCCACA GAGGAAAACA TACAAGGTAC
    CAAGAATGCA ACCCTTCTCT GACTCAAGGG TCCTCTGAAA TTAAGAGATC CTCAAGGAAT
    CATGAGAAGA GCAAAGGCAG ATATCACCAC AGAGATCCTC AGCTCTTGCA AAGTCTTAGG
    AAAAATGAAA TAATGAAGAA GACATTTTCA GAAACAGATT CCAGCACTGA AATACTGGGA
    GTTCCAGAAG GCAGCAAGGA CATGAATGAT GCAGGGCTCC AGGTGAATAA CCCTGTTCAG
    AAGCCTCCTG CCACCTATGA CGATGGGTCT GATAATTTAG AAGTATGCAG AATCTGTCAC
    TGCGAAGGGG ATGAAGAGAG CCCCCTCATC ACACCCTGTC GCTGCACTGG GACACTGCGC
    TTTGTCCACC AGTCCTGCCT CCACCAGTGG ATAAAGAGCT CAGATACACG CTGCTGTGAG
    CTCTGCAAGT ATGACTTCAT AATGGAGACC AAGCTCAAAC CCCTCCGGAA GTGGGAGAAA
    CTACAGATGA CCACAAGTGA AAGGAGGAAA ATATTCTGCT CTGTCACATT CCACGTAATC
    GCGATCACCT GTGTGGTTTG GTCTTTGTAT GTATTGATAG ACCGGACAGC GGAGGAAATC
    AAGCAAGGCA ATGACAATGG TGTCCTTGAA TGGCCATTTT GGACAAAACT GGTTGTGGTA
    GCCATTGGCT TCACAGGAGG TCTTGTCTTC ATGTACGTAC AGTGTAAAGT CTATGTTCAG
    TTGTGGCGCA GGCTGAAGGC CTACAACCGT GTGATCTTTG TACAAAATTG CCCAGACACT
    GCCAAAAAAC TGGAGAAGAA CTTCTCATGT AATGTAAACA CAGACATCAA AGATGCTGTG
    GTAGTGCCTG TACCACAAAC AGGTGCAAAT TCACTGCCAT CTGCAGAGGG TGGCCCCCCT
    GAAGTTGTAT CAGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011530357.1
    CDS122..1759
    Translation

    Target ORF information:

    RefSeq Version XM_011532055.3
    Organism Homo sapiens(human)
    Definition Homo sapiens membrane associated ring-CH-type finger 1 (MARCHF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011532055.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGCTGGGCT GGTGTGAAGC GATAGCCCGT AACCCTCACA GAATTCCAAA CAACACGCGA 
    ACACCCGAGA TCTCAGGGGA TTTGGCTGAC GCCTCACAAA CCTCCACATT GAATGAAAAA
    TCCCCAGGGC GATCTGCAAG TCGATCAAGT AACATTTCAA AAGCAAGCAG CCCAACAACA
    GGGACAGCTC CCAGGAGCCA GTCAAGGTTG TCTGTCTGTC CATCCACTCA GGACATCTGC
    AGATCTGCCA TCTTGCATGA CATGTCAGAA GAGTCATTTG AGTATTGCAC CCCTGTCATG
    GTGCTGAGCC CTGCTAGGAA GGAGTCTGGT AAGAAATCAG TAATACAAAG ACCTAGGAGG
    AGGAGAAAAG CCTCTGAGAG ATATGAGCAT GCTGCAGAAG AACAAATAAG AGGGAGAAAA
    AATGACTTTC ACCTTCAAAT CTCAAGCCCC AGGTGGAGGG AGCTTTACAC AGATTCTTCA
    GATTCATCTT CCACAGATGA GAGTCATTGG ATTCAGGCAA AAAGAAGAGC CCAGGTTAAA
    TTCAGACTGT CAAGAAGAAG GAGGAGAAAT AATAATAAGC CGTGTGAAAA CTTAGCTGGG
    TCTTCCACTC CTAACGGAAT TGAGCTCGTT GATCTGGGAT CCAAAGGTAA AGAGCAACAA
    GAGCTGATTG AATGTGAGAG TTGCTCTTTA AATCTCCACA GAGGAAAACA TACAAGGTAC
    CAAGAATGCA ACCCTTCTCT GACTCAAGGG TCCTCTGAAA TTAAGAGATC CTCAAGGAAT
    CATGAGAAGA GCAAAGGCAG ATATCACCAC AGAGATCCTC AGCTCTTGCA AAGTCTTAGG
    AAAAATGAAA TAATGAAGAA GACATTTTCA GAAACAGATT CCAGCACTGA AATACTGGGA
    GTTCCAGAAG GCAGCAAGGA CATGAATGAT GCAGGGCTCC AGGTGAATAA CCCTGTTCAG
    AAGCCTCCTG CCACCTATGA CGATGGGTCT GATAATTTAG AAGTATGCAG AATCTGTCAC
    TGCGAAGGGG ATGAAGAGAG CCCCCTCATC ACACCCTGTC GCTGCACTGG GACACTGCGC
    TTTGTCCACC AGTCCTGCCT CCACCAGTGG ATAAAGAGCT CAGATACACG CTGCTGTGAG
    CTCTGCAAGT ATGACTTCAT AATGGAGACC AAGCTCAAAC CCCTCCGGAA GTGGGAGAAA
    CTACAGATGA CCACAAGTGA AAGGAGGAAA ATATTCTGCT CTGTCACATT CCACGTAATC
    GCGATCACCT GTGTGGTTTG GTCTTTGTAT GTATTGATAG ACCGGACAGC GGAGGAAATC
    AAGCAAGGCA ATGACAATGG TGTCCTTGAA TGGCCATTTT GGACAAAACT GGTTGTGGTA
    GCCATTGGCT TCACAGGAGG TCTTGTCTTC ATGTACGTAC AGTGTAAAGT CTATGTTCAG
    TTGTGGCGCA GGCTGAAGGC CTACAACCGT GTGATCTTTG TACAAAATTG CCCAGACACT
    GCCAAAAAAC TGGAGAAGAA CTTCTCATGT AATGTAAACA CAGACATCAA AGATGCTGTG
    GTAGTGCCTG TACCACAAAC AGGTGCAAAT TCACTGCCAT CTGCAGAGGG TGGCCCCCCT
    GAAGTTGTAT CAGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95249
    Clone ID Related Accession (Same CDS sequence) XM_017008335.1 , XM_017008336.1
    Accession Version XM_017008335.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 858bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase MARCH1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGCCCCTCC ACCAGATTTC CGTAATCCCA GCACGGGAGA CTGCCAGCAA TGGGAGAAAT 
    TCAATGGGCA GAAACAAAGA GAAGAACAAG GAAGTCGAGA ATGAAAAATC CCCAGGGCGA
    TCTGCAAGTC GATCAAGTAA CATTTCAAAA GCAAGCAGCC CAACAACAGG GACAGCTCCC
    AGGAGCCAGT CAAGGTTGTC TGTCTGTCCA TCCACTCAGG ACATCTGCAG AATCTGTCAC
    TGCGAAGGGG ATGAAGAGAG CCCCCTCATC ACACCCTGTC GCTGCACTGG GACACTGCGC
    TTTGTCCACC AGTCCTGCCT CCACCAGTGG ATAAAGAGCT CAGATACACG CTGCTGTGAG
    CTCTGCAAGT ATGACTTCAT AATGGAGACC AAGCTCAAAC CCCTCCGGAA GTGGGAGAAA
    CTACAGATGA CCACAAGTGA AAGGAGGAAA ATATTCTGCT CTGTCACATT CCACGTAATC
    GCGATCACCT GTGTGGTTTG GTCTTTGTAT GTATTGATAG ACCGGACAGC GGAGGAAATC
    AAGCAAGGCA ATGACAATGG TGTCCTTGAA TGGCCATTTT GGACAAAACT GGTTGTGGTA
    GCCATTGGCT TCACAGGAGG TCTTGTCTTC ATGTACGTAC AGTGTAAAGT CTATGTTCAG
    TTGTGGCGCA GGCTGAAGGC CTACAACCGT GTGATCTTTG TACAAAATTG CCCAGACACT
    GCCAAAAAAC TGGAGAAGAA CTTCTCATGT AATGTAAACA CAGACATCAA AGATGCTGTG
    GTAGTGCCTG TACCACAAAC AGGTGCAAAT TCACTGCCAT CTGCAGAGGG TGGCCCCCCT
    GAAGTTGTAT CAGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016863824.1
    CDS501..1358
    Translation

    Target ORF information:

    RefSeq Version XM_017008335.1
    Organism Homo sapiens(human)
    Definition Homo sapiens membrane associated ring-CH-type finger 1 (MARCHF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008335.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGCCCCTCC ACCAGATTTC CGTAATCCCA GCACGGGAGA CTGCCAGCAA TGGGAGAAAT 
    TCAATGGGCA GAAACAAAGA GAAGAACAAG GAAGTCGAGA ATGAAAAATC CCCAGGGCGA
    TCTGCAAGTC GATCAAGTAA CATTTCAAAA GCAAGCAGCC CAACAACAGG GACAGCTCCC
    AGGAGCCAGT CAAGGTTGTC TGTCTGTCCA TCCACTCAGG ACATCTGCAG AATCTGTCAC
    TGCGAAGGGG ATGAAGAGAG CCCCCTCATC ACACCCTGTC GCTGCACTGG GACACTGCGC
    TTTGTCCACC AGTCCTGCCT CCACCAGTGG ATAAAGAGCT CAGATACACG CTGCTGTGAG
    CTCTGCAAGT ATGACTTCAT AATGGAGACC AAGCTCAAAC CCCTCCGGAA GTGGGAGAAA
    CTACAGATGA CCACAAGTGA AAGGAGGAAA ATATTCTGCT CTGTCACATT CCACGTAATC
    GCGATCACCT GTGTGGTTTG GTCTTTGTAT GTATTGATAG ACCGGACAGC GGAGGAAATC
    AAGCAAGGCA ATGACAATGG TGTCCTTGAA TGGCCATTTT GGACAAAACT GGTTGTGGTA
    GCCATTGGCT TCACAGGAGG TCTTGTCTTC ATGTACGTAC AGTGTAAAGT CTATGTTCAG
    TTGTGGCGCA GGCTGAAGGC CTACAACCGT GTGATCTTTG TACAAAATTG CCCAGACACT
    GCCAAAAAAC TGGAGAAGAA CTTCTCATGT AATGTAAACA CAGACATCAA AGATGCTGTG
    GTAGTGCCTG TACCACAAAC AGGTGCAAAT TCACTGCCAT CTGCAGAGGG TGGCCCCCCT
    GAAGTTGTAT CAGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95249
    Clone ID Related Accession (Same CDS sequence) XM_017008335.1 , XM_017008336.1
    Accession Version XM_017008336.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 858bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase MARCH1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGCCCCTCC ACCAGATTTC CGTAATCCCA GCACGGGAGA CTGCCAGCAA TGGGAGAAAT 
    TCAATGGGCA GAAACAAAGA GAAGAACAAG GAAGTCGAGA ATGAAAAATC CCCAGGGCGA
    TCTGCAAGTC GATCAAGTAA CATTTCAAAA GCAAGCAGCC CAACAACAGG GACAGCTCCC
    AGGAGCCAGT CAAGGTTGTC TGTCTGTCCA TCCACTCAGG ACATCTGCAG AATCTGTCAC
    TGCGAAGGGG ATGAAGAGAG CCCCCTCATC ACACCCTGTC GCTGCACTGG GACACTGCGC
    TTTGTCCACC AGTCCTGCCT CCACCAGTGG ATAAAGAGCT CAGATACACG CTGCTGTGAG
    CTCTGCAAGT ATGACTTCAT AATGGAGACC AAGCTCAAAC CCCTCCGGAA GTGGGAGAAA
    CTACAGATGA CCACAAGTGA AAGGAGGAAA ATATTCTGCT CTGTCACATT CCACGTAATC
    GCGATCACCT GTGTGGTTTG GTCTTTGTAT GTATTGATAG ACCGGACAGC GGAGGAAATC
    AAGCAAGGCA ATGACAATGG TGTCCTTGAA TGGCCATTTT GGACAAAACT GGTTGTGGTA
    GCCATTGGCT TCACAGGAGG TCTTGTCTTC ATGTACGTAC AGTGTAAAGT CTATGTTCAG
    TTGTGGCGCA GGCTGAAGGC CTACAACCGT GTGATCTTTG TACAAAATTG CCCAGACACT
    GCCAAAAAAC TGGAGAAGAA CTTCTCATGT AATGTAAACA CAGACATCAA AGATGCTGTG
    GTAGTGCCTG TACCACAAAC AGGTGCAAAT TCACTGCCAT CTGCAGAGGG TGGCCCCCCT
    GAAGTTGTAT CAGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016863825.1
    CDS367..1224
    Translation

    Target ORF information:

    RefSeq Version XM_017008336.1
    Organism Homo sapiens(human)
    Definition Homo sapiens membrane associated ring-CH-type finger 1 (MARCHF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008336.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGCCCCTCC ACCAGATTTC CGTAATCCCA GCACGGGAGA CTGCCAGCAA TGGGAGAAAT 
    TCAATGGGCA GAAACAAAGA GAAGAACAAG GAAGTCGAGA ATGAAAAATC CCCAGGGCGA
    TCTGCAAGTC GATCAAGTAA CATTTCAAAA GCAAGCAGCC CAACAACAGG GACAGCTCCC
    AGGAGCCAGT CAAGGTTGTC TGTCTGTCCA TCCACTCAGG ACATCTGCAG AATCTGTCAC
    TGCGAAGGGG ATGAAGAGAG CCCCCTCATC ACACCCTGTC GCTGCACTGG GACACTGCGC
    TTTGTCCACC AGTCCTGCCT CCACCAGTGG ATAAAGAGCT CAGATACACG CTGCTGTGAG
    CTCTGCAAGT ATGACTTCAT AATGGAGACC AAGCTCAAAC CCCTCCGGAA GTGGGAGAAA
    CTACAGATGA CCACAAGTGA AAGGAGGAAA ATATTCTGCT CTGTCACATT CCACGTAATC
    GCGATCACCT GTGTGGTTTG GTCTTTGTAT GTATTGATAG ACCGGACAGC GGAGGAAATC
    AAGCAAGGCA ATGACAATGG TGTCCTTGAA TGGCCATTTT GGACAAAACT GGTTGTGGTA
    GCCATTGGCT TCACAGGAGG TCTTGTCTTC ATGTACGTAC AGTGTAAAGT CTATGTTCAG
    TTGTGGCGCA GGCTGAAGGC CTACAACCGT GTGATCTTTG TACAAAATTG CCCAGACACT
    GCCAAAAAAC TGGAGAAGAA CTTCTCATGT AATGTAAACA CAGACATCAA AGATGCTGTG
    GTAGTGCCTG TACCACAAAC AGGTGCAAAT TCACTGCCAT CTGCAGAGGG TGGCCCCCCT
    GAAGTTGTAT CAGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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