SMPD4 cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following SMPD4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMPD4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu89644 XM_017004449.2
Latest version!
Homo sapiens sphingomyelin phosphodiesterase 4 (SMPD4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu02896 NM_017751.4
Latest version!
Homo sapiens sphingomyelin phosphodiesterase 4 (SMPD4), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $594.30
$849.00
OHu11302 NM_001171083.2
Latest version!
Homo sapiens sphingomyelin phosphodiesterase 4 (SMPD4), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $538.30
$769.00
OHu12300 NM_017951.4
Latest version!
Homo sapiens sphingomyelin phosphodiesterase 4 (SMPD4), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $615.30
$879.00
OHu69549 XM_011511443.2
Latest version!
Homo sapiens sphingomyelin phosphodiesterase 4 (SMPD4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu69550 XM_011511444.3
Latest version!
Homo sapiens sphingomyelin phosphodiesterase 4 (SMPD4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu69551 XM_011511445.2
Latest version!
Homo sapiens sphingomyelin phosphodiesterase 4 (SMPD4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu89643 XM_024452978.1
Latest version!
Homo sapiens sphingomyelin phosphodiesterase 4 (SMPD4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OHu89643 XM_017004448.2
Latest version!
Homo sapiens sphingomyelin phosphodiesterase 4 (SMPD4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
View Old Accession Versions >>
OHu69550 XM_011511444.2 Homo sapiens sphingomyelin phosphodiesterase 4 (SMPD4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu89643 XM_017004448.1 Homo sapiens sphingomyelin phosphodiesterase 4 (SMPD4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OHu89644 XM_017004449.1 Homo sapiens sphingomyelin phosphodiesterase 4 (SMPD4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu89644
    Clone ID Related Accession (Same CDS sequence) XM_017004449.2 , XM_017004449.1
    Accession Version XM_017004449.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1287bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product sphingomyelin phosphodiesterase 4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017004449.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGTGGCTGA GCTACCTGCA GCCGTGGCGG TACGCGCCTG ACAAGCAGGC TCCGGGCAGC 
    GACTCCCAGC CCCGGTGTGT GTCGGAGAAA TGGGCACCCT TTGTCCAGGA GAACCTGCTG
    ATGTACACCA AGTTGTTTGT GGGCTTTCTG AACCGCGCGC TCCGCACAGA CCTGGTCAGC
    CCCAAGCACG CGCTCATGGT GTTCCGAGTG GCCAAAGTCT TTGCCCAGCC CAACCTGGCT
    GAGATGATTC AGAAAGGTGA GCAGCTATTC CTGGAGCCAG AGCTGGTCAT CCCCCACCGC
    CAGCACCGAC TCTTCACGGC CCCCACATTC ACTGGGAGCT TCCTGTCACC CTGGCCACCA
    GCGGTCACTG ATGCCTCCTT CAAGGTGAAG AGCCACGTCT ACAGCCTGGA GGGCCAGGAC
    TGCAAGTACA CCCCGATGTT TGGGCCCGAG GCCCGCACCC TGGTCCTGCG CCTCGCTCAG
    CTCATCACAC AGGCCAAACA CACAGCCAAG TCCATCTCCG ACCAGTGTGC GGAGAGCCCG
    GCTGGCCACT CCTTCCTCTC ATGGCTGGGC TTTAGCTCCA TGGACACCAA TGGCTCCTAC
    ACAGCCAACG ACCTGGACGA GATGGGGCAA GACAGTGTCC GGAAGACAGA TGAATACCTG
    GAGAAGGCCC TGGAGTACCT GCGCCAGATA TTCCGGCTCA GCGAAGCGCA GCTCAGGCAG
    TTCACACTCG CCTTGGGCAC CACCCAGGAT GAGAATGGAA AAAAGCAACT CCCCGACTGC
    ATCGTGGGTG AGGACGGACT CATCCTTACG CCCCTGGGGC GGTACCAGAT CATCAATGGG
    CTGCGAAGGT TTGAAATTGA GTACCAGGGG GACCCGGAGC TGCAGCCCAT CCGGAGCTAT
    GAGATCGCCA GCTTGGTCCG CACACTCTTT AGGCTGTCGT CTGCCATCAA CCACAGATTT
    GCAGGACAGA TGGCGGCTCT GTGTTCCCGG GATGACTTCC TCGGCAGCTT CTGTCGCTAC
    CACCTCACAG AACCTGGGCT GGCCAGCAGG CACCTGCTGA GCCCTGTGGG GCGGAGGCAG
    GTGGCCGGCC ACACCCGCGG CCCCAGGCTC AGCCTGCGCT TCCTGGGCAG TTACCGGACG
    CTGGTCTCGC TGCTGCTGGC CTTCTTCGTG GCCTCTCTGT TCTGCGTCGG GCCCCTCCCA
    TGCACGCTGC TGCTCACCCT GGGCTATGTC CTCTACGCCT CTGCCATGAC ACTGCTGACC
    GAGCGGGGGA AGCTGCACCA GCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016859938.1
    CDS2994..4280
    Translation

    Target ORF information:

    RefSeq Version XM_017004449.2
    Organism Homo sapiens(human)
    Definition Homo sapiens sphingomyelin phosphodiesterase 4 (SMPD4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017004449.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGTGGCTGA GCTACCTGCA GCCGTGGCGG TACGCGCCTG ACAAGCAGGC TCCGGGCAGC 
    GACTCCCAGC CCCGGTGTGT GTCGGAGAAA TGGGCACCCT TTGTCCAGGA GAACCTGCTG
    ATGTACACCA AGTTGTTTGT GGGCTTTCTG AACCGCGCGC TCCGCACAGA CCTGGTCAGC
    CCCAAGCACG CGCTCATGGT GTTCCGAGTG GCCAAAGTCT TTGCCCAGCC CAACCTGGCT
    GAGATGATTC AGAAAGGTGA GCAGCTATTC CTGGAGCCAG AGCTGGTCAT CCCCCACCGC
    CAGCACCGAC TCTTCACGGC CCCCACATTC ACTGGGAGCT TCCTGTCACC CTGGCCACCA
    GCGGTCACTG ATGCCTCCTT CAAGGTGAAG AGCCACGTCT ACAGCCTGGA GGGCCAGGAC
    TGCAAGTACA CCCCGATGTT TGGGCCCGAG GCCCGCACCC TGGTCCTGCG CCTCGCTCAG
    CTCATCACAC AGGCCAAACA CACAGCCAAG TCCATCTCCG ACCAGTGTGC GGAGAGCCCG
    GCTGGCCACT CCTTCCTCTC ATGGCTGGGC TTTAGCTCCA TGGACACCAA TGGCTCCTAC
    ACAGCCAACG ACCTGGACGA GATGGGGCAA GACAGTGTCC GGAAGACAGA TGAATACCTG
    GAGAAGGCCC TGGAGTACCT GCGCCAGATA TTCCGGCTCA GCGAAGCGCA GCTCAGGCAG
    TTCACACTCG CCTTGGGCAC CACCCAGGAT GAGAATGGAA AAAAGCAACT CCCCGACTGC
    ATCGTGGGTG AGGACGGACT CATCCTTACG CCCCTGGGGC GGTACCAGAT CATCAATGGG
    CTGCGAAGGT TTGAAATTGA GTACCAGGGG GACCCGGAGC TGCAGCCCAT CCGGAGCTAT
    GAGATCGCCA GCTTGGTCCG CACACTCTTT AGGCTGTCGT CTGCCATCAA CCACAGATTT
    GCAGGACAGA TGGCGGCTCT GTGTTCCCGG GATGACTTCC TCGGCAGCTT CTGTCGCTAC
    CACCTCACAG AACCTGGGCT GGCCAGCAGG CACCTGCTGA GCCCTGTGGG GCGGAGGCAG
    GTGGCCGGCC ACACCCGCGG CCCCAGGCTC AGCCTGCGCT TCCTGGGCAG TTACCGGACG
    CTGGTCTCGC TGCTGCTGGC CTTCTTCGTG GCCTCTCTGT TCTGCGTCGG GCCCCTCCCA
    TGCACGCTGC TGCTCACCCT GGGCTATGTC CTCTACGCCT CTGCCATGAC ACTGCTGACC
    GAGCGGGGGA AGCTGCACCA GCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu89644
    Clone ID Related Accession (Same CDS sequence) XM_017004449.2 , XM_017004449.1
    Accession Version XM_017004449.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1287bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product sphingomyelin phosphodiesterase 4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGTGGCTGA GCTACCTGCA GCCGTGGCGG TACGCGCCTG ACAAGCAGGC TCCGGGCAGC 
    GACTCCCAGC CCCGGTGTGT GTCGGAGAAA TGGGCACCCT TTGTCCAGGA GAACCTGCTG
    ATGTACACCA AGTTGTTTGT GGGCTTTCTG AACCGCGCGC TCCGCACAGA CCTGGTCAGC
    CCCAAGCACG CGCTCATGGT GTTCCGAGTG GCCAAAGTCT TTGCCCAGCC CAACCTGGCT
    GAGATGATTC AGAAAGGTGA GCAGCTATTC CTGGAGCCAG AGCTGGTCAT CCCCCACCGC
    CAGCACCGAC TCTTCACGGC CCCCACATTC ACTGGGAGCT TCCTGTCACC CTGGCCACCA
    GCGGTCACTG ATGCCTCCTT CAAGGTGAAG AGCCACGTCT ACAGCCTGGA GGGCCAGGAC
    TGCAAGTACA CCCCGATGTT TGGGCCCGAG GCCCGCACCC TGGTCCTGCG CCTCGCTCAG
    CTCATCACAC AGGCCAAACA CACAGCCAAG TCCATCTCCG ACCAGTGTGC GGAGAGCCCG
    GCTGGCCACT CCTTCCTCTC ATGGCTGGGC TTTAGCTCCA TGGACACCAA TGGCTCCTAC
    ACAGCCAACG ACCTGGACGA GATGGGGCAA GACAGTGTCC GGAAGACAGA TGAATACCTG
    GAGAAGGCCC TGGAGTACCT GCGCCAGATA TTCCGGCTCA GCGAAGCGCA GCTCAGGCAG
    TTCACACTCG CCTTGGGCAC CACCCAGGAT GAGAATGGAA AAAAGCAACT CCCCGACTGC
    ATCGTGGGTG AGGACGGACT CATCCTTACG CCCCTGGGGC GGTACCAGAT CATCAATGGG
    CTGCGAAGGT TTGAAATTGA GTACCAGGGG GACCCGGAGC TGCAGCCCAT CCGGAGCTAT
    GAGATCGCCA GCTTGGTCCG CACACTCTTT AGGCTGTCGT CTGCCATCAA CCACAGATTT
    GCAGGACAGA TGGCGGCTCT GTGTTCCCGG GATGACTTCC TCGGCAGCTT CTGTCGCTAC
    CACCTCACAG AACCTGGGCT GGCCAGCAGG CACCTGCTGA GCCCTGTGGG GCGGAGGCAG
    GTGGCCGGCC ACACCCGCGG CCCCAGGCTC AGCCTGCGCT TCCTGGGCAG TTACCGGACG
    CTGGTCTCGC TGCTGCTGGC CTTCTTCGTG GCCTCTCTGT TCTGCGTCGG GCCCCTCCCA
    TGCACGCTGC TGCTCACCCT GGGCTATGTC CTCTACGCCT CTGCCATGAC ACTGCTGACC
    GAGCGGGGGA AGCTGCACCA GCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016859938.1
    CDS3046..4332
    Translation

    Target ORF information:

    RefSeq Version XM_017004449.1
    Organism Homo sapiens(human)
    Definition Homo sapiens sphingomyelin phosphodiesterase 4 (SMPD4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017004449.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGTGGCTGA GCTACCTGCA GCCGTGGCGG TACGCGCCTG ACAAGCAGGC TCCGGGCAGC 
    GACTCCCAGC CCCGGTGTGT GTCGGAGAAA TGGGCACCCT TTGTCCAGGA GAACCTGCTG
    ATGTACACCA AGTTGTTTGT GGGCTTTCTG AACCGCGCGC TCCGCACAGA CCTGGTCAGC
    CCCAAGCACG CGCTCATGGT GTTCCGAGTG GCCAAAGTCT TTGCCCAGCC CAACCTGGCT
    GAGATGATTC AGAAAGGTGA GCAGCTATTC CTGGAGCCAG AGCTGGTCAT CCCCCACCGC
    CAGCACCGAC TCTTCACGGC CCCCACATTC ACTGGGAGCT TCCTGTCACC CTGGCCACCA
    GCGGTCACTG ATGCCTCCTT CAAGGTGAAG AGCCACGTCT ACAGCCTGGA GGGCCAGGAC
    TGCAAGTACA CCCCGATGTT TGGGCCCGAG GCCCGCACCC TGGTCCTGCG CCTCGCTCAG
    CTCATCACAC AGGCCAAACA CACAGCCAAG TCCATCTCCG ACCAGTGTGC GGAGAGCCCG
    GCTGGCCACT CCTTCCTCTC ATGGCTGGGC TTTAGCTCCA TGGACACCAA TGGCTCCTAC
    ACAGCCAACG ACCTGGACGA GATGGGGCAA GACAGTGTCC GGAAGACAGA TGAATACCTG
    GAGAAGGCCC TGGAGTACCT GCGCCAGATA TTCCGGCTCA GCGAAGCGCA GCTCAGGCAG
    TTCACACTCG CCTTGGGCAC CACCCAGGAT GAGAATGGAA AAAAGCAACT CCCCGACTGC
    ATCGTGGGTG AGGACGGACT CATCCTTACG CCCCTGGGGC GGTACCAGAT CATCAATGGG
    CTGCGAAGGT TTGAAATTGA GTACCAGGGG GACCCGGAGC TGCAGCCCAT CCGGAGCTAT
    GAGATCGCCA GCTTGGTCCG CACACTCTTT AGGCTGTCGT CTGCCATCAA CCACAGATTT
    GCAGGACAGA TGGCGGCTCT GTGTTCCCGG GATGACTTCC TCGGCAGCTT CTGTCGCTAC
    CACCTCACAG AACCTGGGCT GGCCAGCAGG CACCTGCTGA GCCCTGTGGG GCGGAGGCAG
    GTGGCCGGCC ACACCCGCGG CCCCAGGCTC AGCCTGCGCT TCCTGGGCAG TTACCGGACG
    CTGGTCTCGC TGCTGCTGGC CTTCTTCGTG GCCTCTCTGT TCTGCGTCGG GCCCCTCCCA
    TGCACGCTGC TGCTCACCCT GGGCTATGTC CTCTACGCCT CTGCCATGAC ACTGCTGACC
    GAGCGGGGGA AGCTGCACCA GCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02896
    Clone ID Related Accession (Same CDS sequence) NM_017751.4
    Accession Version NM_017751.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2514bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-27
    Organism Homo sapiens(human)
    Product sphingomyelin phosphodiesterase 4 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA797873.1, AK000304.1 and AI393912.1. On Jul 7, 2010 this sequence version replaced NM_017751.3. Transcript Variant: This variant (1) lacks an alternate in-frame exon in the central coding region, compared to variant 2, resulting in an isoform (1) that is shorter than isoform 2. CCDS Note: This CCDS representation uses the 5'-most in-frame start codon. While this start codon is well-conserved, it should be noted that other than human and chimp, all other species have indels that follow this start codon. Other primates and rodents have frameshifting indels, and thus cannot use this start codon. However, some mammalian species, including cow, pig and dog, contain additional compensating indels that bring them back in frame; these species could therefore use the start codon represented in this CCDS. An alternative downstream start codon, which is more widely conserved and has a stronger Kozak signal, also exists. It is possible that leaky scanning by ribosomes would allow the downstream start codon to be used, at least some of the time. The use of the downstream start codon would result in a protein that is 39 aa shorter at the N-terminus. There is no experimental evidence showing which start codon is preferentially used in vivo. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.343486.1, SRR1660803.180218.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGACGACTT TCGGCGCCGT GGCGGAATGG CGGCTTCCAT CTCTGAGGCG AGCGACGCTA 
    TGGATCCCAC AGTGGTTTGC TAAGAAGGCC ATTTTCAACT CTCCACTGGA GGCTGCTATG
    GCGTTCCCTC ACCTGCAGCA GCCCAGCTTT CTACTGGCTA GCCTGAAAGC TGACTCTATA
    AATAAGCCCT TTGCACAGCA GTGCCAAGAC TTGGTTAAAG TCATTGAGGA CTTTCCAGCA
    AAGGAGCTGC ACACCATCTT CCCATGGCTG GTAGAAAGCA TTTTTGGCAG CCTAGATGGT
    GTCCTCGTTG GCTGGAACCT CCGCTGCTTA CAGGGGCGCG TGAATCCTGT GGAGTACAGC
    ATCGTGATGG AATTTCTCGA CCCTGGTGGC CCAATGATGA AGTTGGTTTA TAAGCTTCAA
    GCTGAAGACT ATAAGTTCGA CTTTCCTGTC TCCTACTTGC CTGGTCCTGT GAAGGCGTCC
    ATCCAGGAGT GCATCCTCCC TGACAGTCCT CTGTACCACA ACAAGGTCCA GTTCACCCCT
    ACTGGGGGCC TTGGTCTGAA CTTGGCCCTG AATCCGTTCG AGTATTACAT ATTCTTCTTT
    GCCTTGAGCC TCATCACTCA GAAGCCACTT CCTGTGTCCC TCCACGTCCG TACTTCAGAC
    TGTGCCTATT TCATCCTGGT GGACAGGTAC CTGTCATGGT TCCTGCCCAC CGAAGGCAGT
    GTGCCCCCAC CACTCTCCTC CAGCCCAGGG GGGACCAGCC CCTCACCACC TCCCAGGACA
    CCAGCCATAC CCTTTGCTTC CTATGGCCTC CACCACACTA GCCTCCTAAA GCGACACATC
    TCTCATCAGA CGTCTGTGAA TGCAGACCCC GCCTCCCACG AGATCTGGAG GTCAGAAACT
    CTGCTCCAGG TTTTTGTTGA AATGTGGCTT CATCACTATT CCTTGGAGAT GTATCAAAAA
    ATGCAGTCCC CTCATGCCAA GGAGTCGTTC ACGCCTACTG AGGAGCATGT GTTGGTGGTG
    CGCCTGCTGC TGAAGCACCT GCACGCCTTT GCCAACAGCC TGAAGCCAGA GCAGGCCTCA
    CCCTCCGCCC ACTCCCACGC CACCAGCCCC CTGGAGGAGT TCAAACGGGC TGCTGTCCCG
    AGGTTCGTCC AGCAGAAACT CTACCTCTTC TTGCAGCATT GCTTTGGCCA CTGGCCCCTG
    GACGCATCGT TCAGAGCTGT CCTGGAGATG TGGCTGAGCT ACCTGCAGCC GTGGCGGTAC
    GCGCCTGACA AGCAGGCTCC GGGCAGCGAC TCCCAGCCCC GGTGTGTGTC GGAGAAATGG
    GCACCCTTTG TCCAGGAGAA CCTGCTGATG TACACCAAGT TGTTTGTGGG CTTTCTGAAC
    CGCGCGCTCC GCACAGACCT GGTCAGCCCC AAGCACGCGC TCATGGTGTT CCGAGTGGCC
    AAAGTCTTTG CCCAGCCCAA CCTGGCTGAG ATGATTCAGA AAGGTGAGCA GCTATTCCTG
    GAGCCAGAGC TGGTCATCCC CCACCGCCAG CACCGACTCT TCACGGCCCC CACATTCACT
    GGGAGCTTCC TGTCACCCTG GCCACCAGCG GTCACTGATG CCTCCTTCAA GGTGAAGAGC
    CACGTCTACA GCCTGGAGGG CCAGGACTGC AAGTACACCC CGATGTTTGG GCCCGAGGCC
    CGCACCCTGG TCCTGCGCCT CGCTCAGCTC ATCACACAGG CCAAACACAC AGCCAAGTCC
    ATCTCCGACC AGTGTGCGGA GAGCCCGGCT GGCCACTCCT TCCTCTCATG GCTGGGCTTT
    AGCTCCATGG ACACCAATGG CTCCTACACA GCCAACGACC TGGACGAGAT GGGGCAAGAC
    AGTGTCCGGA AGACAGATGA ATACCTGGAG AAGGCCCTGG AGTACCTGCG CCAGATATTC
    CGGCTCAGCG AAGCGCAGCT CAGGCAGTTC ACACTCGCCT TGGGCACCAC CCAGGATGAG
    AATGGAAAAA AGCAACTCCC CGACTGCATC GTGGGTGAGG ACGGACTCAT CCTTACGCCC
    CTGGGGCGGT ACCAGATCAT CAATGGGCTG CGAAGGTTTG AAATTGAGTA CCAGGGGGAC
    CCGGAGCTGC AGCCCATCCG GAGCTATGAG ATCGCCAGCT TGGTCCGCAC ACTCTTTAGG
    CTGTCGTCTG CCATCAACCA CAGATTTGCA GGACAGATGG CGGCTCTGTG TTCCCGGGAT
    GACTTCCTCG GCAGCTTCTG TCGCTACCAC CTCACAGAAC CTGGGCTGGC CAGCAGGCAC
    CTGCTGAGCC CTGTGGGGCG GAGGCAGGTG GCCGGCCACA CCCGCGGCCC CAGGCTCAGC
    CTGCGCTTCC TGGGCAGTTA CCGGACGCTG GTCTCGCTGC TGCTGGCCTT CTTCGTGGCC
    TCTCTGTTCT GCGTCGGGCC CCTCCCATGC ACGCTGCTGC TCACCCTGGG CTATGTCCTC
    TACGCCTCTG CCATGACACT GCTGACCGAG CGGGGGAAGC TGCACCAGCC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_060221.2
    CDS157..2670
    Translation

    Target ORF information:

    RefSeq Version NM_017751.4
    Organism Homo sapiens(human)
    Definition Homo sapiens sphingomyelin phosphodiesterase 4 (SMPD4), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_017751.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGACGACTT TCGGCGCCGT GGCGGAATGG CGGCTTCCAT CTCTGAGGCG AGCGACGCTA 
    TGGATCCCAC AGTGGTTTGC TAAGAAGGCC ATTTTCAACT CTCCACTGGA GGCTGCTATG
    GCGTTCCCTC ACCTGCAGCA GCCCAGCTTT CTACTGGCTA GCCTGAAAGC TGACTCTATA
    AATAAGCCCT TTGCACAGCA GTGCCAAGAC TTGGTTAAAG TCATTGAGGA CTTTCCAGCA
    AAGGAGCTGC ACACCATCTT CCCATGGCTG GTAGAAAGCA TTTTTGGCAG CCTAGATGGT
    GTCCTCGTTG GCTGGAACCT CCGCTGCTTA CAGGGGCGCG TGAATCCTGT GGAGTACAGC
    ATCGTGATGG AATTTCTCGA CCCTGGTGGC CCAATGATGA AGTTGGTTTA TAAGCTTCAA
    GCTGAAGACT ATAAGTTCGA CTTTCCTGTC TCCTACTTGC CTGGTCCTGT GAAGGCGTCC
    ATCCAGGAGT GCATCCTCCC TGACAGTCCT CTGTACCACA ACAAGGTCCA GTTCACCCCT
    ACTGGGGGCC TTGGTCTGAA CTTGGCCCTG AATCCGTTCG AGTATTACAT ATTCTTCTTT
    GCCTTGAGCC TCATCACTCA GAAGCCACTT CCTGTGTCCC TCCACGTCCG TACTTCAGAC
    TGTGCCTATT TCATCCTGGT GGACAGGTAC CTGTCATGGT TCCTGCCCAC CGAAGGCAGT
    GTGCCCCCAC CACTCTCCTC CAGCCCAGGG GGGACCAGCC CCTCACCACC TCCCAGGACA
    CCAGCCATAC CCTTTGCTTC CTATGGCCTC CACCACACTA GCCTCCTAAA GCGACACATC
    TCTCATCAGA CGTCTGTGAA TGCAGACCCC GCCTCCCACG AGATCTGGAG GTCAGAAACT
    CTGCTCCAGG TTTTTGTTGA AATGTGGCTT CATCACTATT CCTTGGAGAT GTATCAAAAA
    ATGCAGTCCC CTCATGCCAA GGAGTCGTTC ACGCCTACTG AGGAGCATGT GTTGGTGGTG
    CGCCTGCTGC TGAAGCACCT GCACGCCTTT GCCAACAGCC TGAAGCCAGA GCAGGCCTCA
    CCCTCCGCCC ACTCCCACGC CACCAGCCCC CTGGAGGAGT TCAAACGGGC TGCTGTCCCG
    AGGTTCGTCC AGCAGAAACT CTACCTCTTC TTGCAGCATT GCTTTGGCCA CTGGCCCCTG
    GACGCATCGT TCAGAGCTGT CCTGGAGATG TGGCTGAGCT ACCTGCAGCC GTGGCGGTAC
    GCGCCTGACA AGCAGGCTCC GGGCAGCGAC TCCCAGCCCC GGTGTGTGTC GGAGAAATGG
    GCACCCTTTG TCCAGGAGAA CCTGCTGATG TACACCAAGT TGTTTGTGGG CTTTCTGAAC
    CGCGCGCTCC GCACAGACCT GGTCAGCCCC AAGCACGCGC TCATGGTGTT CCGAGTGGCC
    AAAGTCTTTG CCCAGCCCAA CCTGGCTGAG ATGATTCAGA AAGGTGAGCA GCTATTCCTG
    GAGCCAGAGC TGGTCATCCC CCACCGCCAG CACCGACTCT TCACGGCCCC CACATTCACT
    GGGAGCTTCC TGTCACCCTG GCCACCAGCG GTCACTGATG CCTCCTTCAA GGTGAAGAGC
    CACGTCTACA GCCTGGAGGG CCAGGACTGC AAGTACACCC CGATGTTTGG GCCCGAGGCC
    CGCACCCTGG TCCTGCGCCT CGCTCAGCTC ATCACACAGG CCAAACACAC AGCCAAGTCC
    ATCTCCGACC AGTGTGCGGA GAGCCCGGCT GGCCACTCCT TCCTCTCATG GCTGGGCTTT
    AGCTCCATGG ACACCAATGG CTCCTACACA GCCAACGACC TGGACGAGAT GGGGCAAGAC
    AGTGTCCGGA AGACAGATGA ATACCTGGAG AAGGCCCTGG AGTACCTGCG CCAGATATTC
    CGGCTCAGCG AAGCGCAGCT CAGGCAGTTC ACACTCGCCT TGGGCACCAC CCAGGATGAG
    AATGGAAAAA AGCAACTCCC CGACTGCATC GTGGGTGAGG ACGGACTCAT CCTTACGCCC
    CTGGGGCGGT ACCAGATCAT CAATGGGCTG CGAAGGTTTG AAATTGAGTA CCAGGGGGAC
    CCGGAGCTGC AGCCCATCCG GAGCTATGAG ATCGCCAGCT TGGTCCGCAC ACTCTTTAGG
    CTGTCGTCTG CCATCAACCA CAGATTTGCA GGACAGATGG CGGCTCTGTG TTCCCGGGAT
    GACTTCCTCG GCAGCTTCTG TCGCTACCAC CTCACAGAAC CTGGGCTGGC CAGCAGGCAC
    CTGCTGAGCC CTGTGGGGCG GAGGCAGGTG GCCGGCCACA CCCGCGGCCC CAGGCTCAGC
    CTGCGCTTCC TGGGCAGTTA CCGGACGCTG GTCTCGCTGC TGCTGGCCTT CTTCGTGGCC
    TCTCTGTTCT GCGTCGGGCC CCTCCCATGC ACGCTGCTGC TCACCCTGGG CTATGTCCTC
    TACGCCTCTG CCATGACACT GCTGACCGAG CGGGGGAAGC TGCACCAGCC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu11302
    Clone ID Related Accession (Same CDS sequence) NM_001171083.2
    Accession Version NM_001171083.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2295bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-27
    Organism Homo sapiens(human)
    Product sphingomyelin phosphodiesterase 4 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA797873.1, AK299185.1, AC018804.9 and AI393912.1. On Jul 7, 2010 this sequence version replaced NM_001171083.1. Transcript Variant: This variant (3) lacks two alternate exons resulting in the loss of an in-frame segment in the 5' coding region, and it also lacks an alternate in-frame exon in the central coding region, compared to variant 2. The resulting isoform (3) is shorter than isoform 2. ##Evidence-Data-START## Transcript exon combination :: AK299185.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGACGACTT TCGGCGCCGT GGCGGAATGG CGGCTTCCAT CTCTGAGGCG AGCGACGCTA 
    TGGATCCCAC AGTGGTTTGC TAAGAAGGCC ATTTTCAACT CTCCACTGGA GGCTGCTATG
    GCGTTCCCTC ACCTGCAGCA GCCCAGCTTT CTACTGGCTA GCCTGAAAGC TGACTCTATA
    AATAAGCCCT TTGCACAGCA GTGCCAAGAC TTGGTTAAAG TCATTGAGGA CTTTCCAGCA
    AAGGGTCCTG TGAAGGCGTC CATCCAGGAG TGCATCCTCC CTGACAGTCC TCTGTACCAC
    AACAAGGTCC AGTTCACCCC TACTGGGGGC CTTGGTCTGA ACTTGGCCCT GAATCCGTTC
    GAGTATTACA TATTCTTCTT TGCCTTGAGC CTCATCACTC AGAAGCCACT TCCTGTGTCC
    CTCCACGTCC GTACTTCAGA CTGTGCCTAT TTCATCCTGG TGGACAGGTA CCTGTCATGG
    TTCCTGCCCA CCGAAGGCAG TGTGCCCCCA CCACTCTCCT CCAGCCCAGG GGGGACCAGC
    CCCTCACCAC CTCCCAGGAC ACCAGCCATA CCCTTTGCTT CCTATGGCCT CCACCACACT
    AGCCTCCTAA AGCGACACAT CTCTCATCAG ACGTCTGTGA ATGCAGACCC CGCCTCCCAC
    GAGATCTGGA GGTCAGAAAC TCTGCTCCAG GTTTTTGTTG AAATGTGGCT TCATCACTAT
    TCCTTGGAGA TGTATCAAAA AATGCAGTCC CCTCATGCCA AGGAGTCGTT CACGCCTACT
    GAGGAGCATG TGTTGGTGGT GCGCCTGCTG CTGAAGCACC TGCACGCCTT TGCCAACAGC
    CTGAAGCCAG AGCAGGCCTC ACCCTCCGCC CACTCCCACG CCACCAGCCC CCTGGAGGAG
    TTCAAACGGG CTGCTGTCCC GAGGTTCGTC CAGCAGAAAC TCTACCTCTT CTTGCAGCAT
    TGCTTTGGCC ACTGGCCCCT GGACGCATCG TTCAGAGCTG TCCTGGAGAT GTGGCTGAGC
    TACCTGCAGC CGTGGCGGTA CGCGCCTGAC AAGCAGGCTC CGGGCAGCGA CTCCCAGCCC
    CGGTGTGTGT CGGAGAAATG GGCACCCTTT GTCCAGGAGA ACCTGCTGAT GTACACCAAG
    TTGTTTGTGG GCTTTCTGAA CCGCGCGCTC CGCACAGACC TGGTCAGCCC CAAGCACGCG
    CTCATGGTGT TCCGAGTGGC CAAAGTCTTT GCCCAGCCCA ACCTGGCTGA GATGATTCAG
    AAAGGTGAGC AGCTATTCCT GGAGCCAGAG CTGGTCATCC CCCACCGCCA GCACCGACTC
    TTCACGGCCC CCACATTCAC TGGGAGCTTC CTGTCACCCT GGCCACCAGC GGTCACTGAT
    GCCTCCTTCA AGGTGAAGAG CCACGTCTAC AGCCTGGAGG GCCAGGACTG CAAGTACACC
    CCGATGTTTG GGCCCGAGGC CCGCACCCTG GTCCTGCGCC TCGCTCAGCT CATCACACAG
    GCCAAACACA CAGCCAAGTC CATCTCCGAC CAGTGTGCGG AGAGCCCGGC TGGCCACTCC
    TTCCTCTCAT GGCTGGGCTT TAGCTCCATG GACACCAATG GCTCCTACAC AGCCAACGAC
    CTGGACGAGA TGGGGCAAGA CAGTGTCCGG AAGACAGATG AATACCTGGA GAAGGCCCTG
    GAGTACCTGC GCCAGATATT CCGGCTCAGC GAAGCGCAGC TCAGGCAGTT CACACTCGCC
    TTGGGCACCA CCCAGGATGA GAATGGAAAA AAGCAACTCC CCGACTGCAT CGTGGGTGAG
    GACGGACTCA TCCTTACGCC CCTGGGGCGG TACCAGATCA TCAATGGGCT GCGAAGGTTT
    GAAATTGAGT ACCAGGGGGA CCCGGAGCTG CAGCCCATCC GGAGCTATGA GATCGCCAGC
    TTGGTCCGCA CACTCTTTAG GCTGTCGTCT GCCATCAACC ACAGATTTGC AGGACAGATG
    GCGGCTCTGT GTTCCCGGGA TGACTTCCTC GGCAGCTTCT GTCGCTACCA CCTCACAGAA
    CCTGGGCTGG CCAGCAGGCA CCTGCTGAGC CCTGTGGGGC GGAGGCAGGT GGCCGGCCAC
    ACCCGCGGCC CCAGGCTCAG CCTGCGCTTC CTGGGCAGTT ACCGGACGCT GGTCTCGCTG
    CTGCTGGCCT TCTTCGTGGC CTCTCTGTTC TGCGTCGGGC CCCTCCCATG CACGCTGCTG
    CTCACCCTGG GCTATGTCCT CTACGCCTCT GCCATGACAC TGCTGACCGA GCGGGGGAAG
    CTGCACCAGC CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001164554.1
    CDS157..2451
    Translation

    Target ORF information:

    RefSeq Version NM_001171083.2
    Organism Homo sapiens(human)
    Definition Homo sapiens sphingomyelin phosphodiesterase 4 (SMPD4), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001171083.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGACGACTT TCGGCGCCGT GGCGGAATGG CGGCTTCCAT CTCTGAGGCG AGCGACGCTA 
    TGGATCCCAC AGTGGTTTGC TAAGAAGGCC ATTTTCAACT CTCCACTGGA GGCTGCTATG
    GCGTTCCCTC ACCTGCAGCA GCCCAGCTTT CTACTGGCTA GCCTGAAAGC TGACTCTATA
    AATAAGCCCT TTGCACAGCA GTGCCAAGAC TTGGTTAAAG TCATTGAGGA CTTTCCAGCA
    AAGGGTCCTG TGAAGGCGTC CATCCAGGAG TGCATCCTCC CTGACAGTCC TCTGTACCAC
    AACAAGGTCC AGTTCACCCC TACTGGGGGC CTTGGTCTGA ACTTGGCCCT GAATCCGTTC
    GAGTATTACA TATTCTTCTT TGCCTTGAGC CTCATCACTC AGAAGCCACT TCCTGTGTCC
    CTCCACGTCC GTACTTCAGA CTGTGCCTAT TTCATCCTGG TGGACAGGTA CCTGTCATGG
    TTCCTGCCCA CCGAAGGCAG TGTGCCCCCA CCACTCTCCT CCAGCCCAGG GGGGACCAGC
    CCCTCACCAC CTCCCAGGAC ACCAGCCATA CCCTTTGCTT CCTATGGCCT CCACCACACT
    AGCCTCCTAA AGCGACACAT CTCTCATCAG ACGTCTGTGA ATGCAGACCC CGCCTCCCAC
    GAGATCTGGA GGTCAGAAAC TCTGCTCCAG GTTTTTGTTG AAATGTGGCT TCATCACTAT
    TCCTTGGAGA TGTATCAAAA AATGCAGTCC CCTCATGCCA AGGAGTCGTT CACGCCTACT
    GAGGAGCATG TGTTGGTGGT GCGCCTGCTG CTGAAGCACC TGCACGCCTT TGCCAACAGC
    CTGAAGCCAG AGCAGGCCTC ACCCTCCGCC CACTCCCACG CCACCAGCCC CCTGGAGGAG
    TTCAAACGGG CTGCTGTCCC GAGGTTCGTC CAGCAGAAAC TCTACCTCTT CTTGCAGCAT
    TGCTTTGGCC ACTGGCCCCT GGACGCATCG TTCAGAGCTG TCCTGGAGAT GTGGCTGAGC
    TACCTGCAGC CGTGGCGGTA CGCGCCTGAC AAGCAGGCTC CGGGCAGCGA CTCCCAGCCC
    CGGTGTGTGT CGGAGAAATG GGCACCCTTT GTCCAGGAGA ACCTGCTGAT GTACACCAAG
    TTGTTTGTGG GCTTTCTGAA CCGCGCGCTC CGCACAGACC TGGTCAGCCC CAAGCACGCG
    CTCATGGTGT TCCGAGTGGC CAAAGTCTTT GCCCAGCCCA ACCTGGCTGA GATGATTCAG
    AAAGGTGAGC AGCTATTCCT GGAGCCAGAG CTGGTCATCC CCCACCGCCA GCACCGACTC
    TTCACGGCCC CCACATTCAC TGGGAGCTTC CTGTCACCCT GGCCACCAGC GGTCACTGAT
    GCCTCCTTCA AGGTGAAGAG CCACGTCTAC AGCCTGGAGG GCCAGGACTG CAAGTACACC
    CCGATGTTTG GGCCCGAGGC CCGCACCCTG GTCCTGCGCC TCGCTCAGCT CATCACACAG
    GCCAAACACA CAGCCAAGTC CATCTCCGAC CAGTGTGCGG AGAGCCCGGC TGGCCACTCC
    TTCCTCTCAT GGCTGGGCTT TAGCTCCATG GACACCAATG GCTCCTACAC AGCCAACGAC
    CTGGACGAGA TGGGGCAAGA CAGTGTCCGG AAGACAGATG AATACCTGGA GAAGGCCCTG
    GAGTACCTGC GCCAGATATT CCGGCTCAGC GAAGCGCAGC TCAGGCAGTT CACACTCGCC
    TTGGGCACCA CCCAGGATGA GAATGGAAAA AAGCAACTCC CCGACTGCAT CGTGGGTGAG
    GACGGACTCA TCCTTACGCC CCTGGGGCGG TACCAGATCA TCAATGGGCT GCGAAGGTTT
    GAAATTGAGT ACCAGGGGGA CCCGGAGCTG CAGCCCATCC GGAGCTATGA GATCGCCAGC
    TTGGTCCGCA CACTCTTTAG GCTGTCGTCT GCCATCAACC ACAGATTTGC AGGACAGATG
    GCGGCTCTGT GTTCCCGGGA TGACTTCCTC GGCAGCTTCT GTCGCTACCA CCTCACAGAA
    CCTGGGCTGG CCAGCAGGCA CCTGCTGAGC CCTGTGGGGC GGAGGCAGGT GGCCGGCCAC
    ACCCGCGGCC CCAGGCTCAG CCTGCGCTTC CTGGGCAGTT ACCGGACGCT GGTCTCGCTG
    CTGCTGGCCT TCTTCGTGGC CTCTCTGTTC TGCGTCGGGC CCCTCCCATG CACGCTGCTG
    CTCACCCTGG GCTATGTCCT CTACGCCTCT GCCATGACAC TGCTGACCGA GCGGGGGAAG
    CTGCACCAGC CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu12300
    Clone ID Related Accession (Same CDS sequence) NM_017951.4
    Accession Version NM_017951.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2601bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Homo sapiens(human)
    Product sphingomyelin phosphodiesterase 4 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA797873.1, EU367941.1, AC018804.9 and AI393912.1. This sequence is a reference standard in the RefSeqGene project. On or before Mar 22, 2019 this sequence version replaced NR_033230.2, NM_017951.3. Transcript Variant: This variant (2) encodes the longest isoform (2). CCDS Note: This CCDS representation uses the 5'-most in-frame start codon. While this start codon is well-conserved, it should be noted that other than human and chimp, all other species have indels that follow this start codon. Other primates and rodents have frameshifting indels, and thus cannot use this start codon. However, some mammalian species, including cow, pig and dog, contain additional compensating indels that bring them back in frame; these species could therefore use the start codon represented in this CCDS. An alternative downstream start codon, which is more widely conserved and has a stronger Kozak signal, also exists. It is possible that leaky scanning by ribosomes would allow the downstream start codon to be used, at least some of the time. The use of the downstream start codon would result in a protein that is 39 aa shorter at the N-terminus. There is no experimental evidence showing which start codon is preferentially used in vivo. ##Evidence-Data-START## Transcript exon combination :: AB037839.1, SRR1660809.133443.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGACGACTT TCGGCGCCGT GGCGGAATGG CGGCTTCCAT CTCTGAGGCG AGCGACGCTA 
    TGGATCCCAC AGTGGTTTGC TAAGAAGGCC ATTTTCAACT CTCCACTGGA GGCTGCTATG
    GCGTTCCCTC ACCTGCAGCA GCCCAGCTTT CTACTGGCTA GCCTGAAAGC TGACTCTATA
    AATAAGCCCT TTGCACAGCA GTGCCAAGAC TTGGTTAAAG TCATTGAGGA CTTTCCAGCA
    AAGGAGCTGC ACACCATCTT CCCATGGCTG GTAGAAAGCA TTTTTGGCAG CCTAGATGGT
    GTCCTCGTTG GCTGGAACCT CCGCTGCTTA CAGGGGCGCG TGAATCCTGT GGAGTACAGC
    ATCGTGATGG AATTTCTCGA CCCTGGTGGC CCAATGATGA AGTTGGTTTA TAAGCTTCAA
    GCTGAAGACT ATAAGTTCGA CTTTCCTGTC TCCTACTTGC CTGGTCCTGT GAAGGCGTCC
    ATCCAGGAGT GCATCCTCCC TGACAGTCCT CTGTACCACA ACAAGGTCCA GTTCACCCCT
    ACTGGGGGCC TTGGTCTGAA CTTGGCCCTG AATCCGTTCG AGTATTACAT ATTCTTCTTT
    GCCTTGAGCC TCATCACTCA GAAGCCACTT CCTGTGTCCC TCCACGTCCG TACTTCAGAC
    TGTGCCTATT TCATCCTGGT GGACAGGTAC CTGTCATGGT TCCTGCCCAC CGAAGGCAGT
    GTGCCCCCAC CACTCTCCTC CAGCCCAGGG GGGACCAGCC CCTCACCACC TCCCAGGACA
    CCAGCCATAC CCTTTGCTTC CTATGGCCTC CACCACACTA GCCTCCTAAA GCGACACATC
    TCTCATCAGA CGTCTGTGAA TGCAGACCCC GCCTCCCACG AGATCTGGAG GTCAGAAACT
    CTGCTCCAGG TTTTTGTTGA AATGTGGCTT CATCACTATT CCTTGGAGAT GTATCAAAAA
    ATGCAGTCCC CTCATGCCAA GCTGGAGGTT CTGCACTACC GACTCAGTGT CTCCAGCGCC
    CTCTACAGCC CCGCCCAACC CAGCCTCCAG GCCCTCCACG CCTACCAAGA GTCGTTCACG
    CCTACTGAGG AGCATGTGTT GGTGGTGCGC CTGCTGCTGA AGCACCTGCA CGCCTTTGCC
    AACAGCCTGA AGCCAGAGCA GGCCTCACCC TCCGCCCACT CCCACGCCAC CAGCCCCCTG
    GAGGAGTTCA AACGGGCTGC TGTCCCGAGG TTCGTCCAGC AGAAACTCTA CCTCTTCTTG
    CAGCATTGCT TTGGCCACTG GCCCCTGGAC GCATCGTTCA GAGCTGTCCT GGAGATGTGG
    CTGAGCTACC TGCAGCCGTG GCGGTACGCG CCTGACAAGC AGGCTCCGGG CAGCGACTCC
    CAGCCCCGGT GTGTGTCGGA GAAATGGGCA CCCTTTGTCC AGGAGAACCT GCTGATGTAC
    ACCAAGTTGT TTGTGGGCTT TCTGAACCGC GCGCTCCGCA CAGACCTGGT CAGCCCCAAG
    CACGCGCTCA TGGTGTTCCG AGTGGCCAAA GTCTTTGCCC AGCCCAACCT GGCTGAGATG
    ATTCAGAAAG GTGAGCAGCT ATTCCTGGAG CCAGAGCTGG TCATCCCCCA CCGCCAGCAC
    CGACTCTTCA CGGCCCCCAC ATTCACTGGG AGCTTCCTGT CACCCTGGCC ACCAGCGGTC
    ACTGATGCCT CCTTCAAGGT GAAGAGCCAC GTCTACAGCC TGGAGGGCCA GGACTGCAAG
    TACACCCCGA TGTTTGGGCC CGAGGCCCGC ACCCTGGTCC TGCGCCTCGC TCAGCTCATC
    ACACAGGCCA AACACACAGC CAAGTCCATC TCCGACCAGT GTGCGGAGAG CCCGGCTGGC
    CACTCCTTCC TCTCATGGCT GGGCTTTAGC TCCATGGACA CCAATGGCTC CTACACAGCC
    AACGACCTGG ACGAGATGGG GCAAGACAGT GTCCGGAAGA CAGATGAATA CCTGGAGAAG
    GCCCTGGAGT ACCTGCGCCA GATATTCCGG CTCAGCGAAG CGCAGCTCAG GCAGTTCACA
    CTCGCCTTGG GCACCACCCA GGATGAGAAT GGAAAAAAGC AACTCCCCGA CTGCATCGTG
    GGTGAGGACG GACTCATCCT TACGCCCCTG GGGCGGTACC AGATCATCAA TGGGCTGCGA
    AGGTTTGAAA TTGAGTACCA GGGGGACCCG GAGCTGCAGC CCATCCGGAG CTATGAGATC
    GCCAGCTTGG TCCGCACACT CTTTAGGCTG TCGTCTGCCA TCAACCACAG ATTTGCAGGA
    CAGATGGCGG CTCTGTGTTC CCGGGATGAC TTCCTCGGCA GCTTCTGTCG CTACCACCTC
    ACAGAACCTG GGCTGGCCAG CAGGCACCTG CTGAGCCCTG TGGGGCGGAG GCAGGTGGCC
    GGCCACACCC GCGGCCCCAG GCTCAGCCTG CGCTTCCTGG GCAGTTACCG GACGCTGGTC
    TCGCTGCTGC TGGCCTTCTT CGTGGCCTCT CTGTTCTGCG TCGGGCCCCT CCCATGCACG
    CTGCTGCTCA CCCTGGGCTA TGTCCTCTAC GCCTCTGCCA TGACACTGCT GACCGAGCGG
    GGGAAGCTGC ACCAGCCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_060421.2
    CDS157..2757
    Translation

    Target ORF information:

    RefSeq Version NM_017951.4
    Organism Homo sapiens(human)
    Definition Homo sapiens sphingomyelin phosphodiesterase 4 (SMPD4), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_017951.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGACGACTT TCGGCGCCGT GGCGGAATGG CGGCTTCCAT CTCTGAGGCG AGCGACGCTA 
    TGGATCCCAC AGTGGTTTGC TAAGAAGGCC ATTTTCAACT CTCCACTGGA GGCTGCTATG
    GCGTTCCCTC ACCTGCAGCA GCCCAGCTTT CTACTGGCTA GCCTGAAAGC TGACTCTATA
    AATAAGCCCT TTGCACAGCA GTGCCAAGAC TTGGTTAAAG TCATTGAGGA CTTTCCAGCA
    AAGGAGCTGC ACACCATCTT CCCATGGCTG GTAGAAAGCA TTTTTGGCAG CCTAGATGGT
    GTCCTCGTTG GCTGGAACCT CCGCTGCTTA CAGGGGCGCG TGAATCCTGT GGAGTACAGC
    ATCGTGATGG AATTTCTCGA CCCTGGTGGC CCAATGATGA AGTTGGTTTA TAAGCTTCAA
    GCTGAAGACT ATAAGTTCGA CTTTCCTGTC TCCTACTTGC CTGGTCCTGT GAAGGCGTCC
    ATCCAGGAGT GCATCCTCCC TGACAGTCCT CTGTACCACA ACAAGGTCCA GTTCACCCCT
    ACTGGGGGCC TTGGTCTGAA CTTGGCCCTG AATCCGTTCG AGTATTACAT ATTCTTCTTT
    GCCTTGAGCC TCATCACTCA GAAGCCACTT CCTGTGTCCC TCCACGTCCG TACTTCAGAC
    TGTGCCTATT TCATCCTGGT GGACAGGTAC CTGTCATGGT TCCTGCCCAC CGAAGGCAGT
    GTGCCCCCAC CACTCTCCTC CAGCCCAGGG GGGACCAGCC CCTCACCACC TCCCAGGACA
    CCAGCCATAC CCTTTGCTTC CTATGGCCTC CACCACACTA GCCTCCTAAA GCGACACATC
    TCTCATCAGA CGTCTGTGAA TGCAGACCCC GCCTCCCACG AGATCTGGAG GTCAGAAACT
    CTGCTCCAGG TTTTTGTTGA AATGTGGCTT CATCACTATT CCTTGGAGAT GTATCAAAAA
    ATGCAGTCCC CTCATGCCAA GCTGGAGGTT CTGCACTACC GACTCAGTGT CTCCAGCGCC
    CTCTACAGCC CCGCCCAACC CAGCCTCCAG GCCCTCCACG CCTACCAAGA GTCGTTCACG
    CCTACTGAGG AGCATGTGTT GGTGGTGCGC CTGCTGCTGA AGCACCTGCA CGCCTTTGCC
    AACAGCCTGA AGCCAGAGCA GGCCTCACCC TCCGCCCACT CCCACGCCAC CAGCCCCCTG
    GAGGAGTTCA AACGGGCTGC TGTCCCGAGG TTCGTCCAGC AGAAACTCTA CCTCTTCTTG
    CAGCATTGCT TTGGCCACTG GCCCCTGGAC GCATCGTTCA GAGCTGTCCT GGAGATGTGG
    CTGAGCTACC TGCAGCCGTG GCGGTACGCG CCTGACAAGC AGGCTCCGGG CAGCGACTCC
    CAGCCCCGGT GTGTGTCGGA GAAATGGGCA CCCTTTGTCC AGGAGAACCT GCTGATGTAC
    ACCAAGTTGT TTGTGGGCTT TCTGAACCGC GCGCTCCGCA CAGACCTGGT CAGCCCCAAG
    CACGCGCTCA TGGTGTTCCG AGTGGCCAAA GTCTTTGCCC AGCCCAACCT GGCTGAGATG
    ATTCAGAAAG GTGAGCAGCT ATTCCTGGAG CCAGAGCTGG TCATCCCCCA CCGCCAGCAC
    CGACTCTTCA CGGCCCCCAC ATTCACTGGG AGCTTCCTGT CACCCTGGCC ACCAGCGGTC
    ACTGATGCCT CCTTCAAGGT GAAGAGCCAC GTCTACAGCC TGGAGGGCCA GGACTGCAAG
    TACACCCCGA TGTTTGGGCC CGAGGCCCGC ACCCTGGTCC TGCGCCTCGC TCAGCTCATC
    ACACAGGCCA AACACACAGC CAAGTCCATC TCCGACCAGT GTGCGGAGAG CCCGGCTGGC
    CACTCCTTCC TCTCATGGCT GGGCTTTAGC TCCATGGACA CCAATGGCTC CTACACAGCC
    AACGACCTGG ACGAGATGGG GCAAGACAGT GTCCGGAAGA CAGATGAATA CCTGGAGAAG
    GCCCTGGAGT ACCTGCGCCA GATATTCCGG CTCAGCGAAG CGCAGCTCAG GCAGTTCACA
    CTCGCCTTGG GCACCACCCA GGATGAGAAT GGAAAAAAGC AACTCCCCGA CTGCATCGTG
    GGTGAGGACG GACTCATCCT TACGCCCCTG GGGCGGTACC AGATCATCAA TGGGCTGCGA
    AGGTTTGAAA TTGAGTACCA GGGGGACCCG GAGCTGCAGC CCATCCGGAG CTATGAGATC
    GCCAGCTTGG TCCGCACACT CTTTAGGCTG TCGTCTGCCA TCAACCACAG ATTTGCAGGA
    CAGATGGCGG CTCTGTGTTC CCGGGATGAC TTCCTCGGCA GCTTCTGTCG CTACCACCTC
    ACAGAACCTG GGCTGGCCAG CAGGCACCTG CTGAGCCCTG TGGGGCGGAG GCAGGTGGCC
    GGCCACACCC GCGGCCCCAG GCTCAGCCTG CGCTTCCTGG GCAGTTACCG GACGCTGGTC
    TCGCTGCTGC TGGCCTTCTT CGTGGCCTCT CTGTTCTGCG TCGGGCCCCT CCCATGCACG
    CTGCTGCTCA CCCTGGGCTA TGTCCTCTAC GCCTCTGCCA TGACACTGCT GACCGAGCGG
    GGGAAGCTGC ACCAGCCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu69549
    Clone ID Related Accession (Same CDS sequence) XM_011511443.2
    Accession Version XM_011511443.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2835bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product sphingomyelin phosphodiesterase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011511443.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGACGACTT TCGGCGCCGT GGCGGAATGG CGGCTTCCAT CTCTGAGGCG AGCGACGCTA 
    TGGATCCCAC AGGTGAGTGG CGCGGGATGC GCGCCTGAGA CGGTCCGGCG CCCTGGGGGC
    TTCCCCGAGC CCCTGCGGAG GCCATCTCCG TTCCTCCGCG AGCCCCAGGA CTCGGGCCGG
    CCAGCCGGCT CTGCCCAGGC AGGGCAGGCT GGGGAGTGGA GGGCCCCGGT TGAGTCTTCT
    GCCACCCCTC TGGGCCATGC CTTTGTGCCA CCTTCCCGCG CGGCTCGTAC ACTCTGTCCC
    GGTCCGTGGT TTGCTAAGAA GGCCATTTTC AACTCTCCAC TGGAGGCTGC TATGGCGTTC
    CCTCACCTGC AGCAGCCCAG CTTTCTACTG GCTAGCCTGA AAGCTGACTC TATAAATAAG
    CCCTTTGCAC AGCAGTGCCA AGACTTGGTT AAAGTCATTG AGGACTTTCC AGCAAAGGAG
    CTGCACACCA TCTTCCCATG GCTGGTAGAA AGCATTTTTG GCAGCCTAGA TGGTGTCCTC
    GTTGGCTGGA ACCTCCGCTG CTTACAGGGG CGCGTGAATC CTGTGGAGTA CAGCATCGTG
    ATGGAATTTC TCGACCCTGG TGGCCCAATG ATGAAGTTGG TTTATAAGCT TCAAGCTGAA
    GACTATAAGT TCGACTTTCC TGTCTCCTAC TTGCCTGGTC CTGTGAAGGC GTCCATCCAG
    GAGTGCATCC TCCCTGACAG TCCTCTGTAC CACAACAAGG TCCAGTTCAC CCCTACTGGG
    GGCCTTGGTC TGAACTTGGC CCTGAATCCG TTCGAGTATT ACATATTCTT CTTTGCCTTG
    AGCCTCATCA CTCAGAAGCC ACTTCCTGTG TCCCTCCACG TCCGTACTTC AGACTGTGCC
    TATTTCATCC TGGTGGACAG GTACCTGTCA TGGTTCCTGC CCACCGAAGG CAGTGTGCCC
    CCACCACTCT CCTCCAGCCC AGGGGGGACC AGCCCCTCAC CACCTCCCAG GACACCAGCC
    ATACCCTTTG CTTCCTATGG CCTCCACCAC ACTAGCCTCC TAAAGCGACA CATCTCTCAT
    CAGACGTCTG TGAATGCAGA CCCCGCCTCC CACGAGATCT GGAGGTCAGA AACTCTGCTC
    CAGGTTTTTG TTGAAATGTG GCTTCATCAC TATTCCTTGG AGATGTATCA AAAAATGCAG
    TCCCCTCATG CCAAGCTGGA GGTTCTGCAC TACCGACTCA GTGTCTCCAG CGCCCTCTAC
    AGCCCCGCCC AACCCAGCCT CCAGGCCCTC CACGCCTACC AAGAGTCGTT CACGCCTACT
    GAGGAGCATG TGTTGGTGGT GCGCCTGCTG CTGAAGCACC TGCACGCCTT TGCCAACAGC
    CTGAAGCCAG AGCAGGCCTC ACCCTCCGCC CACTCCCACG CCACCAGCCC CCTGGAGGAG
    TTCAAACGGG CTGCTGTCCC GAGGTTCGTC CAGCAGAAAC TCTACCTCTT CTTGCAGCAT
    TGCTTTGGCC ACTGGCCCCT GGACGCATCG TTCAGAGCTG TCCTGGAGAT GTGGCTGAGC
    TACCTGCAGC CGTGGCGGTA CGCGCCTGAC AAGCAGGCTC CGGGCAGCGA CTCCCAGCCC
    CGGTGTGTGT CGGAGAAATG GGCACCCTTT GTCCAGGAGA ACCTGCTGAT GTACACCAAG
    TTGTTTGTGG GCTTTCTGAA CCGCGCGCTC CGCACAGACC TGGTCAGCCC CAAGCACGCG
    CTCATGGTGT TCCGAGTGGC CAAAGTCTTT GCCCAGCCCA ACCTGGCTGA GATGATTCAG
    AAAGGTGAGC AGCTATTCCT GGAGCCAGAG CTGGTCATCC CCCACCGCCA GCACCGACTC
    TTCACGGCCC CCACATTCAC TGGGAGCTTC CTGTCACCCT GGCCACCAGC GGTCACTGAT
    GCCTCCTTCA AGGTGAAGAG CCACGTCTAC AGCCTGGAGG GCCAGGACTG CAAGTACACC
    CCGATGTTTG GGCCCGAGGC CCGCACCCTG GTCCTGCGCC TCGCTCAGCT CATCACACAG
    GCCAAACACA CAGCCAAGTC CATCTCCGAC CAGTGTGCGG AGAGCCCGGC TGGCCACTCC
    TTCCTCTCAT GGCTGGGCTT TAGCTCCATG GACACCAATG GCTCCTACAC AGCCAACGAC
    CTGGACGAGA TGGGGCAAGA CAGTGTCCGG AAGACAGATG AATACCTGGA GAAGGCCCTG
    GAGTACCTGC GCCAGATATT CCGGCTCAGC GAAGCGCAGC TCAGGCAGTT CACACTCGCC
    TTGGGCACCA CCCAGGATGA GAATGGAAAA AAGCAACTCC CCGACTGCAT CGTGGGTGAG
    GACGGACTCA TCCTTACGCC CCTGGGGCGG TACCAGATCA TCAATGGGCT GCGAAGGTTT
    GAAATTGAGT ACCAGGGGGA CCCGGAGCTG CAGCCCATCC GGAGCTATGA GATCGCCAGC
    TTGGTCCGCA CACTCTTTAG GCTGTCGTCT GCCATCAACC ACAGATTTGC AGGACAGATG
    GCGGCTCTGT GTTCCCGGGA TGACTTCCTC GGCAGCTTCT GTCGCTACCA CCTCACAGAA
    CCTGGGCTGG CCAGCAGGCA CCTGCTGAGC CCTGTGGGGC GGAGGCAGGT GGCCGGCCAC
    ACCCGCGGCC CCAGGCTCAG CCTGCGCTTC CTGGGCAGTT ACCGGACGCT GGTCTCGCTG
    CTGCTGGCCT TCTTCGTGGC CTCTCTGTTC TGCGTCGGGC CCCTCCCATG CACGCTGCTG
    CTCACCCTGG GCTATGTCCT CTACGCCTCT GCCATGACAC TGCTGACCGA GCGGGGGAAG
    CTGCACCAGC CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011509745.1
    CDS85..2919
    Translation

    Target ORF information:

    RefSeq Version XM_011511443.2
    Organism Homo sapiens(human)
    Definition Homo sapiens sphingomyelin phosphodiesterase 4 (SMPD4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011511443.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGACGACTT TCGGCGCCGT GGCGGAATGG CGGCTTCCAT CTCTGAGGCG AGCGACGCTA 
    TGGATCCCAC AGGTGAGTGG CGCGGGATGC GCGCCTGAGA CGGTCCGGCG CCCTGGGGGC
    TTCCCCGAGC CCCTGCGGAG GCCATCTCCG TTCCTCCGCG AGCCCCAGGA CTCGGGCCGG
    CCAGCCGGCT CTGCCCAGGC AGGGCAGGCT GGGGAGTGGA GGGCCCCGGT TGAGTCTTCT
    GCCACCCCTC TGGGCCATGC CTTTGTGCCA CCTTCCCGCG CGGCTCGTAC ACTCTGTCCC
    GGTCCGTGGT TTGCTAAGAA GGCCATTTTC AACTCTCCAC TGGAGGCTGC TATGGCGTTC
    CCTCACCTGC AGCAGCCCAG CTTTCTACTG GCTAGCCTGA AAGCTGACTC TATAAATAAG
    CCCTTTGCAC AGCAGTGCCA AGACTTGGTT AAAGTCATTG AGGACTTTCC AGCAAAGGAG
    CTGCACACCA TCTTCCCATG GCTGGTAGAA AGCATTTTTG GCAGCCTAGA TGGTGTCCTC
    GTTGGCTGGA ACCTCCGCTG CTTACAGGGG CGCGTGAATC CTGTGGAGTA CAGCATCGTG
    ATGGAATTTC TCGACCCTGG TGGCCCAATG ATGAAGTTGG TTTATAAGCT TCAAGCTGAA
    GACTATAAGT TCGACTTTCC TGTCTCCTAC TTGCCTGGTC CTGTGAAGGC GTCCATCCAG
    GAGTGCATCC TCCCTGACAG TCCTCTGTAC CACAACAAGG TCCAGTTCAC CCCTACTGGG
    GGCCTTGGTC TGAACTTGGC CCTGAATCCG TTCGAGTATT ACATATTCTT CTTTGCCTTG
    AGCCTCATCA CTCAGAAGCC ACTTCCTGTG TCCCTCCACG TCCGTACTTC AGACTGTGCC
    TATTTCATCC TGGTGGACAG GTACCTGTCA TGGTTCCTGC CCACCGAAGG CAGTGTGCCC
    CCACCACTCT CCTCCAGCCC AGGGGGGACC AGCCCCTCAC CACCTCCCAG GACACCAGCC
    ATACCCTTTG CTTCCTATGG CCTCCACCAC ACTAGCCTCC TAAAGCGACA CATCTCTCAT
    CAGACGTCTG TGAATGCAGA CCCCGCCTCC CACGAGATCT GGAGGTCAGA AACTCTGCTC
    CAGGTTTTTG TTGAAATGTG GCTTCATCAC TATTCCTTGG AGATGTATCA AAAAATGCAG
    TCCCCTCATG CCAAGCTGGA GGTTCTGCAC TACCGACTCA GTGTCTCCAG CGCCCTCTAC
    AGCCCCGCCC AACCCAGCCT CCAGGCCCTC CACGCCTACC AAGAGTCGTT CACGCCTACT
    GAGGAGCATG TGTTGGTGGT GCGCCTGCTG CTGAAGCACC TGCACGCCTT TGCCAACAGC
    CTGAAGCCAG AGCAGGCCTC ACCCTCCGCC CACTCCCACG CCACCAGCCC CCTGGAGGAG
    TTCAAACGGG CTGCTGTCCC GAGGTTCGTC CAGCAGAAAC TCTACCTCTT CTTGCAGCAT
    TGCTTTGGCC ACTGGCCCCT GGACGCATCG TTCAGAGCTG TCCTGGAGAT GTGGCTGAGC
    TACCTGCAGC CGTGGCGGTA CGCGCCTGAC AAGCAGGCTC CGGGCAGCGA CTCCCAGCCC
    CGGTGTGTGT CGGAGAAATG GGCACCCTTT GTCCAGGAGA ACCTGCTGAT GTACACCAAG
    TTGTTTGTGG GCTTTCTGAA CCGCGCGCTC CGCACAGACC TGGTCAGCCC CAAGCACGCG
    CTCATGGTGT TCCGAGTGGC CAAAGTCTTT GCCCAGCCCA ACCTGGCTGA GATGATTCAG
    AAAGGTGAGC AGCTATTCCT GGAGCCAGAG CTGGTCATCC CCCACCGCCA GCACCGACTC
    TTCACGGCCC CCACATTCAC TGGGAGCTTC CTGTCACCCT GGCCACCAGC GGTCACTGAT
    GCCTCCTTCA AGGTGAAGAG CCACGTCTAC AGCCTGGAGG GCCAGGACTG CAAGTACACC
    CCGATGTTTG GGCCCGAGGC CCGCACCCTG GTCCTGCGCC TCGCTCAGCT CATCACACAG
    GCCAAACACA CAGCCAAGTC CATCTCCGAC CAGTGTGCGG AGAGCCCGGC TGGCCACTCC
    TTCCTCTCAT GGCTGGGCTT TAGCTCCATG GACACCAATG GCTCCTACAC AGCCAACGAC
    CTGGACGAGA TGGGGCAAGA CAGTGTCCGG AAGACAGATG AATACCTGGA GAAGGCCCTG
    GAGTACCTGC GCCAGATATT CCGGCTCAGC GAAGCGCAGC TCAGGCAGTT CACACTCGCC
    TTGGGCACCA CCCAGGATGA GAATGGAAAA AAGCAACTCC CCGACTGCAT CGTGGGTGAG
    GACGGACTCA TCCTTACGCC CCTGGGGCGG TACCAGATCA TCAATGGGCT GCGAAGGTTT
    GAAATTGAGT ACCAGGGGGA CCCGGAGCTG CAGCCCATCC GGAGCTATGA GATCGCCAGC
    TTGGTCCGCA CACTCTTTAG GCTGTCGTCT GCCATCAACC ACAGATTTGC AGGACAGATG
    GCGGCTCTGT GTTCCCGGGA TGACTTCCTC GGCAGCTTCT GTCGCTACCA CCTCACAGAA
    CCTGGGCTGG CCAGCAGGCA CCTGCTGAGC CCTGTGGGGC GGAGGCAGGT GGCCGGCCAC
    ACCCGCGGCC CCAGGCTCAG CCTGCGCTTC CTGGGCAGTT ACCGGACGCT GGTCTCGCTG
    CTGCTGGCCT TCTTCGTGGC CTCTCTGTTC TGCGTCGGGC CCCTCCCATG CACGCTGCTG
    CTCACCCTGG GCTATGTCCT CTACGCCTCT GCCATGACAC TGCTGACCGA GCGGGGGAAG
    CTGCACCAGC CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu69550
    Clone ID Related Accession (Same CDS sequence) XM_011511444.2 , XM_011511444.3
    Accession Version XM_011511444.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2748bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product sphingomyelin phosphodiesterase 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011511444.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGACGACTT TCGGCGCCGT GGCGGAATGG CGGCTTCCAT CTCTGAGGCG AGCGACGCTA 
    TGGATCCCAC AGGTGAGTGG CGCGGGATGC GCGCCTGAGA CGGTCCGGCG CCCTGGGGGC
    TTCCCCGAGC CCCTGCGGAG GCCATCTCCG TTCCTCCGCG AGCCCCAGGA CTCGGGCCGG
    CCAGCCGGCT CTGCCCAGGC AGGGCAGGCT GGGGAGTGGA GGGCCCCGGT TGAGTCTTCT
    GCCACCCCTC TGGGCCATGC CTTTGTGCCA CCTTCCCGCG CGGCTCGTAC ACTCTGTCCC
    GGTCCGTGGT TTGCTAAGAA GGCCATTTTC AACTCTCCAC TGGAGGCTGC TATGGCGTTC
    CCTCACCTGC AGCAGCCCAG CTTTCTACTG GCTAGCCTGA AAGCTGACTC TATAAATAAG
    CCCTTTGCAC AGCAGTGCCA AGACTTGGTT AAAGTCATTG AGGACTTTCC AGCAAAGGAG
    CTGCACACCA TCTTCCCATG GCTGGTAGAA AGCATTTTTG GCAGCCTAGA TGGTGTCCTC
    GTTGGCTGGA ACCTCCGCTG CTTACAGGGG CGCGTGAATC CTGTGGAGTA CAGCATCGTG
    ATGGAATTTC TCGACCCTGG TGGCCCAATG ATGAAGTTGG TTTATAAGCT TCAAGCTGAA
    GACTATAAGT TCGACTTTCC TGTCTCCTAC TTGCCTGGTC CTGTGAAGGC GTCCATCCAG
    GAGTGCATCC TCCCTGACAG TCCTCTGTAC CACAACAAGG TCCAGTTCAC CCCTACTGGG
    GGCCTTGGTC TGAACTTGGC CCTGAATCCG TTCGAGTATT ACATATTCTT CTTTGCCTTG
    AGCCTCATCA CTCAGAAGCC ACTTCCTGTG TCCCTCCACG TCCGTACTTC AGACTGTGCC
    TATTTCATCC TGGTGGACAG GTACCTGTCA TGGTTCCTGC CCACCGAAGG CAGTGTGCCC
    CCACCACTCT CCTCCAGCCC AGGGGGGACC AGCCCCTCAC CACCTCCCAG GACACCAGCC
    ATACCCTTTG CTTCCTATGG CCTCCACCAC ACTAGCCTCC TAAAGCGACA CATCTCTCAT
    CAGACGTCTG TGAATGCAGA CCCCGCCTCC CACGAGATCT GGAGGTCAGA AACTCTGCTC
    CAGGTTTTTG TTGAAATGTG GCTTCATCAC TATTCCTTGG AGATGTATCA AAAAATGCAG
    TCCCCTCATG CCAAGGAGTC GTTCACGCCT ACTGAGGAGC ATGTGTTGGT GGTGCGCCTG
    CTGCTGAAGC ACCTGCACGC CTTTGCCAAC AGCCTGAAGC CAGAGCAGGC CTCACCCTCC
    GCCCACTCCC ACGCCACCAG CCCCCTGGAG GAGTTCAAAC GGGCTGCTGT CCCGAGGTTC
    GTCCAGCAGA AACTCTACCT CTTCTTGCAG CATTGCTTTG GCCACTGGCC CCTGGACGCA
    TCGTTCAGAG CTGTCCTGGA GATGTGGCTG AGCTACCTGC AGCCGTGGCG GTACGCGCCT
    GACAAGCAGG CTCCGGGCAG CGACTCCCAG CCCCGGTGTG TGTCGGAGAA ATGGGCACCC
    TTTGTCCAGG AGAACCTGCT GATGTACACC AAGTTGTTTG TGGGCTTTCT GAACCGCGCG
    CTCCGCACAG ACCTGGTCAG CCCCAAGCAC GCGCTCATGG TGTTCCGAGT GGCCAAAGTC
    TTTGCCCAGC CCAACCTGGC TGAGATGATT CAGAAAGGTG AGCAGCTATT CCTGGAGCCA
    GAGCTGGTCA TCCCCCACCG CCAGCACCGA CTCTTCACGG CCCCCACATT CACTGGGAGC
    TTCCTGTCAC CCTGGCCACC AGCGGTCACT GATGCCTCCT TCAAGGTGAA GAGCCACGTC
    TACAGCCTGG AGGGCCAGGA CTGCAAGTAC ACCCCGATGT TTGGGCCCGA GGCCCGCACC
    CTGGTCCTGC GCCTCGCTCA GCTCATCACA CAGGCCAAAC ACACAGCCAA GTCCATCTCC
    GACCAGTGTG CGGAGAGCCC GGCTGGCCAC TCCTTCCTCT CATGGCTGGG CTTTAGCTCC
    ATGGACACCA ATGGCTCCTA CACAGCCAAC GACCTGGACG AGATGGGGCA AGACAGTGTC
    CGGAAGACAG ATGAATACCT GGAGAAGGCC CTGGAGTACC TGCGCCAGAT ATTCCGGCTC
    AGCGAAGCGC AGCTCAGGCA GTTCACACTC GCCTTGGGCA CCACCCAGGA TGAGAATGGA
    AAAAAGCAAC TCCCCGACTG CATCGTGGGT GAGGACGGAC TCATCCTTAC GCCCCTGGGG
    CGGTACCAGA TCATCAATGG GCTGCGAAGG TTTGAAATTG AGTACCAGGG GGACCCGGAG
    CTGCAGCCCA TCCGGAGCTA TGAGATCGCC AGCTTGGTCC GCACACTCTT TAGGCTGTCG
    TCTGCCATCA ACCACAGATT TGCAGGACAG ATGGCGGCTC TGTGTTCCCG GGATGACTTC
    CTCGGCAGCT TCTGTCGCTA CCACCTCACA GAACCTGGGC TGGCCAGCAG GCACCTGCTG
    AGCCCTGTGG GGCGGAGGCA GGTGGCCGGC CACACCCGCG GCCCCAGGCT CAGCCTGCGC
    TTCCTGGGCA GTTACCGGAC GCTGGTCTCG CTGCTGCTGG CCTTCTTCGT GGCCTCTCTG
    TTCTGCGTCG GGCCCCTCCC ATGCACGCTG CTGCTCACCC TGGGCTATGT CCTCTACGCC
    TCTGCCATGA CACTGCTGAC CGAGCGGGGG AAGCTGCACC AGCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011509746.1
    CDS84..2831
    Translation

    Target ORF information:

    RefSeq Version XM_011511444.2
    Organism Homo sapiens(human)
    Definition Homo sapiens sphingomyelin phosphodiesterase 4 (SMPD4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011511444.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGACGACTT TCGGCGCCGT GGCGGAATGG CGGCTTCCAT CTCTGAGGCG AGCGACGCTA 
    TGGATCCCAC AGGTGAGTGG CGCGGGATGC GCGCCTGAGA CGGTCCGGCG CCCTGGGGGC
    TTCCCCGAGC CCCTGCGGAG GCCATCTCCG TTCCTCCGCG AGCCCCAGGA CTCGGGCCGG
    CCAGCCGGCT CTGCCCAGGC AGGGCAGGCT GGGGAGTGGA GGGCCCCGGT TGAGTCTTCT
    GCCACCCCTC TGGGCCATGC CTTTGTGCCA CCTTCCCGCG CGGCTCGTAC ACTCTGTCCC
    GGTCCGTGGT TTGCTAAGAA GGCCATTTTC AACTCTCCAC TGGAGGCTGC TATGGCGTTC
    CCTCACCTGC AGCAGCCCAG CTTTCTACTG GCTAGCCTGA AAGCTGACTC TATAAATAAG
    CCCTTTGCAC AGCAGTGCCA AGACTTGGTT AAAGTCATTG AGGACTTTCC AGCAAAGGAG
    CTGCACACCA TCTTCCCATG GCTGGTAGAA AGCATTTTTG GCAGCCTAGA TGGTGTCCTC
    GTTGGCTGGA ACCTCCGCTG CTTACAGGGG CGCGTGAATC CTGTGGAGTA CAGCATCGTG
    ATGGAATTTC TCGACCCTGG TGGCCCAATG ATGAAGTTGG TTTATAAGCT TCAAGCTGAA
    GACTATAAGT TCGACTTTCC TGTCTCCTAC TTGCCTGGTC CTGTGAAGGC GTCCATCCAG
    GAGTGCATCC TCCCTGACAG TCCTCTGTAC CACAACAAGG TCCAGTTCAC CCCTACTGGG
    GGCCTTGGTC TGAACTTGGC CCTGAATCCG TTCGAGTATT ACATATTCTT CTTTGCCTTG
    AGCCTCATCA CTCAGAAGCC ACTTCCTGTG TCCCTCCACG TCCGTACTTC AGACTGTGCC
    TATTTCATCC TGGTGGACAG GTACCTGTCA TGGTTCCTGC CCACCGAAGG CAGTGTGCCC
    CCACCACTCT CCTCCAGCCC AGGGGGGACC AGCCCCTCAC CACCTCCCAG GACACCAGCC
    ATACCCTTTG CTTCCTATGG CCTCCACCAC ACTAGCCTCC TAAAGCGACA CATCTCTCAT
    CAGACGTCTG TGAATGCAGA CCCCGCCTCC CACGAGATCT GGAGGTCAGA AACTCTGCTC
    CAGGTTTTTG TTGAAATGTG GCTTCATCAC TATTCCTTGG AGATGTATCA AAAAATGCAG
    TCCCCTCATG CCAAGGAGTC GTTCACGCCT ACTGAGGAGC ATGTGTTGGT GGTGCGCCTG
    CTGCTGAAGC ACCTGCACGC CTTTGCCAAC AGCCTGAAGC CAGAGCAGGC CTCACCCTCC
    GCCCACTCCC ACGCCACCAG CCCCCTGGAG GAGTTCAAAC GGGCTGCTGT CCCGAGGTTC
    GTCCAGCAGA AACTCTACCT CTTCTTGCAG CATTGCTTTG GCCACTGGCC CCTGGACGCA
    TCGTTCAGAG CTGTCCTGGA GATGTGGCTG AGCTACCTGC AGCCGTGGCG GTACGCGCCT
    GACAAGCAGG CTCCGGGCAG CGACTCCCAG CCCCGGTGTG TGTCGGAGAA ATGGGCACCC
    TTTGTCCAGG AGAACCTGCT GATGTACACC AAGTTGTTTG TGGGCTTTCT GAACCGCGCG
    CTCCGCACAG ACCTGGTCAG CCCCAAGCAC GCGCTCATGG TGTTCCGAGT GGCCAAAGTC
    TTTGCCCAGC CCAACCTGGC TGAGATGATT CAGAAAGGTG AGCAGCTATT CCTGGAGCCA
    GAGCTGGTCA TCCCCCACCG CCAGCACCGA CTCTTCACGG CCCCCACATT CACTGGGAGC
    TTCCTGTCAC CCTGGCCACC AGCGGTCACT GATGCCTCCT TCAAGGTGAA GAGCCACGTC
    TACAGCCTGG AGGGCCAGGA CTGCAAGTAC ACCCCGATGT TTGGGCCCGA GGCCCGCACC
    CTGGTCCTGC GCCTCGCTCA GCTCATCACA CAGGCCAAAC ACACAGCCAA GTCCATCTCC
    GACCAGTGTG CGGAGAGCCC GGCTGGCCAC TCCTTCCTCT CATGGCTGGG CTTTAGCTCC
    ATGGACACCA ATGGCTCCTA CACAGCCAAC GACCTGGACG AGATGGGGCA AGACAGTGTC
    CGGAAGACAG ATGAATACCT GGAGAAGGCC CTGGAGTACC TGCGCCAGAT ATTCCGGCTC
    AGCGAAGCGC AGCTCAGGCA GTTCACACTC GCCTTGGGCA CCACCCAGGA TGAGAATGGA
    AAAAAGCAAC TCCCCGACTG CATCGTGGGT GAGGACGGAC TCATCCTTAC GCCCCTGGGG
    CGGTACCAGA TCATCAATGG GCTGCGAAGG TTTGAAATTG AGTACCAGGG GGACCCGGAG
    CTGCAGCCCA TCCGGAGCTA TGAGATCGCC AGCTTGGTCC GCACACTCTT TAGGCTGTCG
    TCTGCCATCA ACCACAGATT TGCAGGACAG ATGGCGGCTC TGTGTTCCCG GGATGACTTC
    CTCGGCAGCT TCTGTCGCTA CCACCTCACA GAACCTGGGC TGGCCAGCAG GCACCTGCTG
    AGCCCTGTGG GGCGGAGGCA GGTGGCCGGC CACACCCGCG GCCCCAGGCT CAGCCTGCGC
    TTCCTGGGCA GTTACCGGAC GCTGGTCTCG CTGCTGCTGG CCTTCTTCGT GGCCTCTCTG
    TTCTGCGTCG GGCCCCTCCC ATGCACGCTG CTGCTCACCC TGGGCTATGT CCTCTACGCC
    TCTGCCATGA CACTGCTGAC CGAGCGGGGG AAGCTGCACC AGCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu69550
    Clone ID Related Accession (Same CDS sequence) XM_011511444.2 , XM_011511444.3
    Accession Version XM_011511444.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2748bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product sphingomyelin phosphodiesterase 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011511444.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGACGACTT TCGGCGCCGT GGCGGAATGG CGGCTTCCAT CTCTGAGGCG AGCGACGCTA 
    TGGATCCCAC AGGTGAGTGG CGCGGGATGC GCGCCTGAGA CGGTCCGGCG CCCTGGGGGC
    TTCCCCGAGC CCCTGCGGAG GCCATCTCCG TTCCTCCGCG AGCCCCAGGA CTCGGGCCGG
    CCAGCCGGCT CTGCCCAGGC AGGGCAGGCT GGGGAGTGGA GGGCCCCGGT TGAGTCTTCT
    GCCACCCCTC TGGGCCATGC CTTTGTGCCA CCTTCCCGCG CGGCTCGTAC ACTCTGTCCC
    GGTCCGTGGT TTGCTAAGAA GGCCATTTTC AACTCTCCAC TGGAGGCTGC TATGGCGTTC
    CCTCACCTGC AGCAGCCCAG CTTTCTACTG GCTAGCCTGA AAGCTGACTC TATAAATAAG
    CCCTTTGCAC AGCAGTGCCA AGACTTGGTT AAAGTCATTG AGGACTTTCC AGCAAAGGAG
    CTGCACACCA TCTTCCCATG GCTGGTAGAA AGCATTTTTG GCAGCCTAGA TGGTGTCCTC
    GTTGGCTGGA ACCTCCGCTG CTTACAGGGG CGCGTGAATC CTGTGGAGTA CAGCATCGTG
    ATGGAATTTC TCGACCCTGG TGGCCCAATG ATGAAGTTGG TTTATAAGCT TCAAGCTGAA
    GACTATAAGT TCGACTTTCC TGTCTCCTAC TTGCCTGGTC CTGTGAAGGC GTCCATCCAG
    GAGTGCATCC TCCCTGACAG TCCTCTGTAC CACAACAAGG TCCAGTTCAC CCCTACTGGG
    GGCCTTGGTC TGAACTTGGC CCTGAATCCG TTCGAGTATT ACATATTCTT CTTTGCCTTG
    AGCCTCATCA CTCAGAAGCC ACTTCCTGTG TCCCTCCACG TCCGTACTTC AGACTGTGCC
    TATTTCATCC TGGTGGACAG GTACCTGTCA TGGTTCCTGC CCACCGAAGG CAGTGTGCCC
    CCACCACTCT CCTCCAGCCC AGGGGGGACC AGCCCCTCAC CACCTCCCAG GACACCAGCC
    ATACCCTTTG CTTCCTATGG CCTCCACCAC ACTAGCCTCC TAAAGCGACA CATCTCTCAT
    CAGACGTCTG TGAATGCAGA CCCCGCCTCC CACGAGATCT GGAGGTCAGA AACTCTGCTC
    CAGGTTTTTG TTGAAATGTG GCTTCATCAC TATTCCTTGG AGATGTATCA AAAAATGCAG
    TCCCCTCATG CCAAGGAGTC GTTCACGCCT ACTGAGGAGC ATGTGTTGGT GGTGCGCCTG
    CTGCTGAAGC ACCTGCACGC CTTTGCCAAC AGCCTGAAGC CAGAGCAGGC CTCACCCTCC
    GCCCACTCCC ACGCCACCAG CCCCCTGGAG GAGTTCAAAC GGGCTGCTGT CCCGAGGTTC
    GTCCAGCAGA AACTCTACCT CTTCTTGCAG CATTGCTTTG GCCACTGGCC CCTGGACGCA
    TCGTTCAGAG CTGTCCTGGA GATGTGGCTG AGCTACCTGC AGCCGTGGCG GTACGCGCCT
    GACAAGCAGG CTCCGGGCAG CGACTCCCAG CCCCGGTGTG TGTCGGAGAA ATGGGCACCC
    TTTGTCCAGG AGAACCTGCT GATGTACACC AAGTTGTTTG TGGGCTTTCT GAACCGCGCG
    CTCCGCACAG ACCTGGTCAG CCCCAAGCAC GCGCTCATGG TGTTCCGAGT GGCCAAAGTC
    TTTGCCCAGC CCAACCTGGC TGAGATGATT CAGAAAGGTG AGCAGCTATT CCTGGAGCCA
    GAGCTGGTCA TCCCCCACCG CCAGCACCGA CTCTTCACGG CCCCCACATT CACTGGGAGC
    TTCCTGTCAC CCTGGCCACC AGCGGTCACT GATGCCTCCT TCAAGGTGAA GAGCCACGTC
    TACAGCCTGG AGGGCCAGGA CTGCAAGTAC ACCCCGATGT TTGGGCCCGA GGCCCGCACC
    CTGGTCCTGC GCCTCGCTCA GCTCATCACA CAGGCCAAAC ACACAGCCAA GTCCATCTCC
    GACCAGTGTG CGGAGAGCCC GGCTGGCCAC TCCTTCCTCT CATGGCTGGG CTTTAGCTCC
    ATGGACACCA ATGGCTCCTA CACAGCCAAC GACCTGGACG AGATGGGGCA AGACAGTGTC
    CGGAAGACAG ATGAATACCT GGAGAAGGCC CTGGAGTACC TGCGCCAGAT ATTCCGGCTC
    AGCGAAGCGC AGCTCAGGCA GTTCACACTC GCCTTGGGCA CCACCCAGGA TGAGAATGGA
    AAAAAGCAAC TCCCCGACTG CATCGTGGGT GAGGACGGAC TCATCCTTAC GCCCCTGGGG
    CGGTACCAGA TCATCAATGG GCTGCGAAGG TTTGAAATTG AGTACCAGGG GGACCCGGAG
    CTGCAGCCCA TCCGGAGCTA TGAGATCGCC AGCTTGGTCC GCACACTCTT TAGGCTGTCG
    TCTGCCATCA ACCACAGATT TGCAGGACAG ATGGCGGCTC TGTGTTCCCG GGATGACTTC
    CTCGGCAGCT TCTGTCGCTA CCACCTCACA GAACCTGGGC TGGCCAGCAG GCACCTGCTG
    AGCCCTGTGG GGCGGAGGCA GGTGGCCGGC CACACCCGCG GCCCCAGGCT CAGCCTGCGC
    TTCCTGGGCA GTTACCGGAC GCTGGTCTCG CTGCTGCTGG CCTTCTTCGT GGCCTCTCTG
    TTCTGCGTCG GGCCCCTCCC ATGCACGCTG CTGCTCACCC TGGGCTATGT CCTCTACGCC
    TCTGCCATGA CACTGCTGAC CGAGCGGGGG AAGCTGCACC AGCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011509746.1
    CDS85..2832
    Translation

    Target ORF information:

    RefSeq Version XM_011511444.3
    Organism Homo sapiens(human)
    Definition Homo sapiens sphingomyelin phosphodiesterase 4 (SMPD4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011511444.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGACGACTT TCGGCGCCGT GGCGGAATGG CGGCTTCCAT CTCTGAGGCG AGCGACGCTA 
    TGGATCCCAC AGGTGAGTGG CGCGGGATGC GCGCCTGAGA CGGTCCGGCG CCCTGGGGGC
    TTCCCCGAGC CCCTGCGGAG GCCATCTCCG TTCCTCCGCG AGCCCCAGGA CTCGGGCCGG
    CCAGCCGGCT CTGCCCAGGC AGGGCAGGCT GGGGAGTGGA GGGCCCCGGT TGAGTCTTCT
    GCCACCCCTC TGGGCCATGC CTTTGTGCCA CCTTCCCGCG CGGCTCGTAC ACTCTGTCCC
    GGTCCGTGGT TTGCTAAGAA GGCCATTTTC AACTCTCCAC TGGAGGCTGC TATGGCGTTC
    CCTCACCTGC AGCAGCCCAG CTTTCTACTG GCTAGCCTGA AAGCTGACTC TATAAATAAG
    CCCTTTGCAC AGCAGTGCCA AGACTTGGTT AAAGTCATTG AGGACTTTCC AGCAAAGGAG
    CTGCACACCA TCTTCCCATG GCTGGTAGAA AGCATTTTTG GCAGCCTAGA TGGTGTCCTC
    GTTGGCTGGA ACCTCCGCTG CTTACAGGGG CGCGTGAATC CTGTGGAGTA CAGCATCGTG
    ATGGAATTTC TCGACCCTGG TGGCCCAATG ATGAAGTTGG TTTATAAGCT TCAAGCTGAA
    GACTATAAGT TCGACTTTCC TGTCTCCTAC TTGCCTGGTC CTGTGAAGGC GTCCATCCAG
    GAGTGCATCC TCCCTGACAG TCCTCTGTAC CACAACAAGG TCCAGTTCAC CCCTACTGGG
    GGCCTTGGTC TGAACTTGGC CCTGAATCCG TTCGAGTATT ACATATTCTT CTTTGCCTTG
    AGCCTCATCA CTCAGAAGCC ACTTCCTGTG TCCCTCCACG TCCGTACTTC AGACTGTGCC
    TATTTCATCC TGGTGGACAG GTACCTGTCA TGGTTCCTGC CCACCGAAGG CAGTGTGCCC
    CCACCACTCT CCTCCAGCCC AGGGGGGACC AGCCCCTCAC CACCTCCCAG GACACCAGCC
    ATACCCTTTG CTTCCTATGG CCTCCACCAC ACTAGCCTCC TAAAGCGACA CATCTCTCAT
    CAGACGTCTG TGAATGCAGA CCCCGCCTCC CACGAGATCT GGAGGTCAGA AACTCTGCTC
    CAGGTTTTTG TTGAAATGTG GCTTCATCAC TATTCCTTGG AGATGTATCA AAAAATGCAG
    TCCCCTCATG CCAAGGAGTC GTTCACGCCT ACTGAGGAGC ATGTGTTGGT GGTGCGCCTG
    CTGCTGAAGC ACCTGCACGC CTTTGCCAAC AGCCTGAAGC CAGAGCAGGC CTCACCCTCC
    GCCCACTCCC ACGCCACCAG CCCCCTGGAG GAGTTCAAAC GGGCTGCTGT CCCGAGGTTC
    GTCCAGCAGA AACTCTACCT CTTCTTGCAG CATTGCTTTG GCCACTGGCC CCTGGACGCA
    TCGTTCAGAG CTGTCCTGGA GATGTGGCTG AGCTACCTGC AGCCGTGGCG GTACGCGCCT
    GACAAGCAGG CTCCGGGCAG CGACTCCCAG CCCCGGTGTG TGTCGGAGAA ATGGGCACCC
    TTTGTCCAGG AGAACCTGCT GATGTACACC AAGTTGTTTG TGGGCTTTCT GAACCGCGCG
    CTCCGCACAG ACCTGGTCAG CCCCAAGCAC GCGCTCATGG TGTTCCGAGT GGCCAAAGTC
    TTTGCCCAGC CCAACCTGGC TGAGATGATT CAGAAAGGTG AGCAGCTATT CCTGGAGCCA
    GAGCTGGTCA TCCCCCACCG CCAGCACCGA CTCTTCACGG CCCCCACATT CACTGGGAGC
    TTCCTGTCAC CCTGGCCACC AGCGGTCACT GATGCCTCCT TCAAGGTGAA GAGCCACGTC
    TACAGCCTGG AGGGCCAGGA CTGCAAGTAC ACCCCGATGT TTGGGCCCGA GGCCCGCACC
    CTGGTCCTGC GCCTCGCTCA GCTCATCACA CAGGCCAAAC ACACAGCCAA GTCCATCTCC
    GACCAGTGTG CGGAGAGCCC GGCTGGCCAC TCCTTCCTCT CATGGCTGGG CTTTAGCTCC
    ATGGACACCA ATGGCTCCTA CACAGCCAAC GACCTGGACG AGATGGGGCA AGACAGTGTC
    CGGAAGACAG ATGAATACCT GGAGAAGGCC CTGGAGTACC TGCGCCAGAT ATTCCGGCTC
    AGCGAAGCGC AGCTCAGGCA GTTCACACTC GCCTTGGGCA CCACCCAGGA TGAGAATGGA
    AAAAAGCAAC TCCCCGACTG CATCGTGGGT GAGGACGGAC TCATCCTTAC GCCCCTGGGG
    CGGTACCAGA TCATCAATGG GCTGCGAAGG TTTGAAATTG AGTACCAGGG GGACCCGGAG
    CTGCAGCCCA TCCGGAGCTA TGAGATCGCC AGCTTGGTCC GCACACTCTT TAGGCTGTCG
    TCTGCCATCA ACCACAGATT TGCAGGACAG ATGGCGGCTC TGTGTTCCCG GGATGACTTC
    CTCGGCAGCT TCTGTCGCTA CCACCTCACA GAACCTGGGC TGGCCAGCAG GCACCTGCTG
    AGCCCTGTGG GGCGGAGGCA GGTGGCCGGC CACACCCGCG GCCCCAGGCT CAGCCTGCGC
    TTCCTGGGCA GTTACCGGAC GCTGGTCTCG CTGCTGCTGG CCTTCTTCGT GGCCTCTCTG
    TTCTGCGTCG GGCCCCTCCC ATGCACGCTG CTGCTCACCC TGGGCTATGT CCTCTACGCC
    TCTGCCATGA CACTGCTGAC CGAGCGGGGG AAGCTGCACC AGCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu69551
    Clone ID Related Accession (Same CDS sequence) XM_011511445.2
    Accession Version XM_011511445.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2208bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product sphingomyelin phosphodiesterase 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011511445.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGATGAAGT TGGTTTATAA GCTTCAAGCT GAAGACTATA AGTTCGACTT TCCTGTCTCC 
    TACTTGCCTG GTCCTGTGAA GGCGTCCATC CAGGAGTGCA TCCTCCCTGA CAGTCCTCTG
    TACCACAACA AGGTCCAGTT CACCCCTACT GGGGGCCTTG GTCTGAACTT GGCCCTGAAT
    CCGTTCGAGT ATTACATATT CTTCTTTGCC TTGAGCCTCA TCACTCAGAA GCCACTTCCT
    GTGTCCCTCC ACGTCCGTAC TTCAGACTGT GCCTATTTCA TCCTGGTGGA CAGGTACCTG
    TCATGGTTCC TGCCCACCGA AGGCAGTGTG CCCCCACCAC TCTCCTCCAG CCCAGGGGGG
    ACCAGCCCCT CACCACCTCC CAGGACACCA GCCATACCCT TTGCTTCCTA TGGCCTCCAC
    CACACTAGCC TCCTAAAGCG ACACATCTCT CATCAGACGT CTGTGAATGC AGACCCCGCC
    TCCCACGAGA TCTGGAGGTC AGAAACTCTG CTCCAGGTTT TTGTTGAAAT GTGGCTTCAT
    CACTATTCCT TGGAGATGTA TCAAAAAATG CAGTCCCCTC ATGCCAAGCT GGAGGTTCTG
    CACTACCGAC TCAGTGTCTC CAGCGCCCTC TACAGCCCCG CCCAACCCAG CCTCCAGGCC
    CTCCACGCCT ACCAAGAGTC GTTCACGCCT ACTGAGGAGC ATGTGTTGGT GGTGCGCCTG
    CTGCTGAAGC ACCTGCACGC CTTTGCCAAC AGCCTGAAGC CAGAGCAGGC CTCACCCTCC
    GCCCACTCCC ACGCCACCAG CCCCCTGGAG GAGTTCAAAC GGGCTGCTGT CCCGAGGTTC
    GTCCAGCAGA AACTCTACCT CTTCTTGCAG CATTGCTTTG GCCACTGGCC CCTGGACGCA
    TCGTTCAGAG CTGTCCTGGA GATGTGGCTG AGCTACCTGC AGCCGTGGCG GTACGCGCCT
    GACAAGCAGG CTCCGGGCAG CGACTCCCAG CCCCGGTGTG TGTCGGAGAA ATGGGCACCC
    TTTGTCCAGG AGAACCTGCT GATGTACACC AAGTTGTTTG TGGGCTTTCT GAACCGCGCG
    CTCCGCACAG ACCTGGTCAG CCCCAAGCAC GCGCTCATGG TGTTCCGAGT GGCCAAAGTC
    TTTGCCCAGC CCAACCTGGC TGAGATGATT CAGAAAGGTG AGCAGCTATT CCTGGAGCCA
    GAGCTGGTCA TCCCCCACCG CCAGCACCGA CTCTTCACGG CCCCCACATT CACTGGGAGC
    TTCCTGTCAC CCTGGCCACC AGCGGTCACT GATGCCTCCT TCAAGGTGAA GAGCCACGTC
    TACAGCCTGG AGGGCCAGGA CTGCAAGTAC ACCCCGATGT TTGGGCCCGA GGCCCGCACC
    CTGGTCCTGC GCCTCGCTCA GCTCATCACA CAGGCCAAAC ACACAGCCAA GTCCATCTCC
    GACCAGTGTG CGGAGAGCCC GGCTGGCCAC TCCTTCCTCT CATGGCTGGG CTTTAGCTCC
    ATGGACACCA ATGGCTCCTA CACAGCCAAC GACCTGGACG AGATGGGGCA AGACAGTGTC
    CGGAAGACAG ATGAATACCT GGAGAAGGCC CTGGAGTACC TGCGCCAGAT ATTCCGGCTC
    AGCGAAGCGC AGCTCAGGCA GTTCACACTC GCCTTGGGCA CCACCCAGGA TGAGAATGGA
    AAAAAGCAAC TCCCCGACTG CATCGTGGGT GAGGACGGAC TCATCCTTAC GCCCCTGGGG
    CGGTACCAGA TCATCAATGG GCTGCGAAGG TTTGAAATTG AGTACCAGGG GGACCCGGAG
    CTGCAGCCCA TCCGGAGCTA TGAGATCGCC AGCTTGGTCC GCACACTCTT TAGGCTGTCG
    TCTGCCATCA ACCACAGATT TGCAGGACAG ATGGCGGCTC TGTGTTCCCG GGATGACTTC
    CTCGGCAGCT TCTGTCGCTA CCACCTCACA GAACCTGGGC TGGCCAGCAG GCACCTGCTG
    AGCCCTGTGG GGCGGAGGCA GGTGGCCGGC CACACCCGCG GCCCCAGGCT CAGCCTGCGC
    TTCCTGGGCA GTTACCGGAC GCTGGTCTCG CTGCTGCTGG CCTTCTTCGT GGCCTCTCTG
    TTCTGCGTCG GGCCCCTCCC ATGCACGCTG CTGCTCACCC TGGGCTATGT CCTCTACGCC
    TCTGCCATGA CACTGCTGAC CGAGCGGGGG AAGCTGCACC AGCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011509747.1
    CDS406..2613
    Translation

    Target ORF information:

    RefSeq Version XM_011511445.2
    Organism Homo sapiens(human)
    Definition Homo sapiens sphingomyelin phosphodiesterase 4 (SMPD4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011511445.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGATGAAGT TGGTTTATAA GCTTCAAGCT GAAGACTATA AGTTCGACTT TCCTGTCTCC 
    TACTTGCCTG GTCCTGTGAA GGCGTCCATC CAGGAGTGCA TCCTCCCTGA CAGTCCTCTG
    TACCACAACA AGGTCCAGTT CACCCCTACT GGGGGCCTTG GTCTGAACTT GGCCCTGAAT
    CCGTTCGAGT ATTACATATT CTTCTTTGCC TTGAGCCTCA TCACTCAGAA GCCACTTCCT
    GTGTCCCTCC ACGTCCGTAC TTCAGACTGT GCCTATTTCA TCCTGGTGGA CAGGTACCTG
    TCATGGTTCC TGCCCACCGA AGGCAGTGTG CCCCCACCAC TCTCCTCCAG CCCAGGGGGG
    ACCAGCCCCT CACCACCTCC CAGGACACCA GCCATACCCT TTGCTTCCTA TGGCCTCCAC
    CACACTAGCC TCCTAAAGCG ACACATCTCT CATCAGACGT CTGTGAATGC AGACCCCGCC
    TCCCACGAGA TCTGGAGGTC AGAAACTCTG CTCCAGGTTT TTGTTGAAAT GTGGCTTCAT
    CACTATTCCT TGGAGATGTA TCAAAAAATG CAGTCCCCTC ATGCCAAGCT GGAGGTTCTG
    CACTACCGAC TCAGTGTCTC CAGCGCCCTC TACAGCCCCG CCCAACCCAG CCTCCAGGCC
    CTCCACGCCT ACCAAGAGTC GTTCACGCCT ACTGAGGAGC ATGTGTTGGT GGTGCGCCTG
    CTGCTGAAGC ACCTGCACGC CTTTGCCAAC AGCCTGAAGC CAGAGCAGGC CTCACCCTCC
    GCCCACTCCC ACGCCACCAG CCCCCTGGAG GAGTTCAAAC GGGCTGCTGT CCCGAGGTTC
    GTCCAGCAGA AACTCTACCT CTTCTTGCAG CATTGCTTTG GCCACTGGCC CCTGGACGCA
    TCGTTCAGAG CTGTCCTGGA GATGTGGCTG AGCTACCTGC AGCCGTGGCG GTACGCGCCT
    GACAAGCAGG CTCCGGGCAG CGACTCCCAG CCCCGGTGTG TGTCGGAGAA ATGGGCACCC
    TTTGTCCAGG AGAACCTGCT GATGTACACC AAGTTGTTTG TGGGCTTTCT GAACCGCGCG
    CTCCGCACAG ACCTGGTCAG CCCCAAGCAC GCGCTCATGG TGTTCCGAGT GGCCAAAGTC
    TTTGCCCAGC CCAACCTGGC TGAGATGATT CAGAAAGGTG AGCAGCTATT CCTGGAGCCA
    GAGCTGGTCA TCCCCCACCG CCAGCACCGA CTCTTCACGG CCCCCACATT CACTGGGAGC
    TTCCTGTCAC CCTGGCCACC AGCGGTCACT GATGCCTCCT TCAAGGTGAA GAGCCACGTC
    TACAGCCTGG AGGGCCAGGA CTGCAAGTAC ACCCCGATGT TTGGGCCCGA GGCCCGCACC
    CTGGTCCTGC GCCTCGCTCA GCTCATCACA CAGGCCAAAC ACACAGCCAA GTCCATCTCC
    GACCAGTGTG CGGAGAGCCC GGCTGGCCAC TCCTTCCTCT CATGGCTGGG CTTTAGCTCC
    ATGGACACCA ATGGCTCCTA CACAGCCAAC GACCTGGACG AGATGGGGCA AGACAGTGTC
    CGGAAGACAG ATGAATACCT GGAGAAGGCC CTGGAGTACC TGCGCCAGAT ATTCCGGCTC
    AGCGAAGCGC AGCTCAGGCA GTTCACACTC GCCTTGGGCA CCACCCAGGA TGAGAATGGA
    AAAAAGCAAC TCCCCGACTG CATCGTGGGT GAGGACGGAC TCATCCTTAC GCCCCTGGGG
    CGGTACCAGA TCATCAATGG GCTGCGAAGG TTTGAAATTG AGTACCAGGG GGACCCGGAG
    CTGCAGCCCA TCCGGAGCTA TGAGATCGCC AGCTTGGTCC GCACACTCTT TAGGCTGTCG
    TCTGCCATCA ACCACAGATT TGCAGGACAG ATGGCGGCTC TGTGTTCCCG GGATGACTTC
    CTCGGCAGCT TCTGTCGCTA CCACCTCACA GAACCTGGGC TGGCCAGCAG GCACCTGCTG
    AGCCCTGTGG GGCGGAGGCA GGTGGCCGGC CACACCCGCG GCCCCAGGCT CAGCCTGCGC
    TTCCTGGGCA GTTACCGGAC GCTGGTCTCG CTGCTGCTGG CCTTCTTCGT GGCCTCTCTG
    TTCTGCGTCG GGCCCCTCCC ATGCACGCTG CTGCTCACCC TGGGCTATGT CCTCTACGCC
    TCTGCCATGA CACTGCTGAC CGAGCGGGGG AAGCTGCACC AGCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu89643
    Clone ID Related Accession (Same CDS sequence) XM_017004448.2 , XM_024452978.1 , XM_017004448.1
    Accession Version XM_017004448.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1824bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product sphingomyelin phosphodiesterase 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017004448.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCAGACCC CGCCTCCCAC GAGATCTGGA GGTCAGAAAC TCTGCTCCAG GTTTTTGTTG 
    AAATGTGGCT TCATCACTAT TCCTTGGAGA TGTATCAAAA AATGCAGTCC CCTCATGCCA
    AGCTGGAGGT TCTGCACTAC CGACTCAGTG TCTCCAGCGC CCTCTACAGC CCCGCCCAAC
    CCAGCCTCCA GGCCCTCCAC GCCTACCAAG TCCCTGGGCA ACAGAGGAAG TGACGCCCGG
    GGCTTCTACT GGGCCGAGTT CTCATCTGGC TGTGTCCAGG ATGAGGAGCA GGAGTCGTTC
    ACGCCTACTG AGGAGCATGT GTTGGTGGTG CGCCTGCTGC TGAAGCACCT GCACGCCTTT
    GCCAACAGCC TGAAGCCAGA GCAGGCCTCA CCCTCCGCCC ACTCCCACGC CACCAGCCCC
    CTGGAGGAGT TCAAACGGGC TGCTGTCCCG AGGTTCGTCC AGCAGAAACT CTACCTCTTC
    TTGCAGCATT GCTTTGGCCA CTGGCCCCTG GACGCATCGT TCAGAGCTGT CCTGGAGATG
    TGGCTGAGCT ACCTGCAGCC GTGGCGGTAC GCGCCTGACA AGCAGGCTCC GGGCAGCGAC
    TCCCAGCCCC GGTGTGTGTC GGAGAAATGG GCACCCTTTG TCCAGGAGAA CCTGCTGATG
    TACACCAAGT TGTTTGTGGG CTTTCTGAAC CGCGCGCTCC GCACAGACCT GGTCAGCCCC
    AAGCACGCGC TCATGGTGTT CCGAGTGGCC AAAGTCTTTG CCCAGCCCAA CCTGGCTGAG
    ATGATTCAGA AAGGTGAGCA GCTATTCCTG GAGCCAGAGC TGGTCATCCC CCACCGCCAG
    CACCGACTCT TCACGGCCCC CACATTCACT GGGAGCTTCC TGTCACCCTG GCCACCAGCG
    GTCACTGATG CCTCCTTCAA GGTGAAGAGC CACGTCTACA GCCTGGAGGG CCAGGACTGC
    AAGTACACCC CGATGTTTGG GCCCGAGGCC CGCACCCTGG TCCTGCGCCT CGCTCAGCTC
    ATCACACAGG CCAAACACAC AGCCAAGTCC ATCTCCGACC AGTGTGCGGA GAGCCCGGCT
    GGCCACTCCT TCCTCTCATG GCTGGGCTTT AGCTCCATGG ACACCAATGG CTCCTACACA
    GCCAACGACC TGGACGAGAT GGGGCAAGAC AGTGTCCGGA AGACAGATGA ATACCTGGAG
    AAGGCCCTGG AGTACCTGCG CCAGATATTC CGGCTCAGCG AAGCGCAGCT CAGGCAGTTC
    ACACTCGCCT TGGGCACCAC CCAGGATGAG AATGGAAAAA AGCAACTCCC CGACTGCATC
    GTGGGTGAGG ACGGACTCAT CCTTACGCCC CTGGGGCGGT ACCAGATCAT CAATGGGCTG
    CGAAGGTTTG AAATTGAGTA CCAGGGGGAC CCGGAGCTGC AGCCCATCCG GAGCTATGAG
    ATCGCCAGCT TGGTCCGCAC ACTCTTTAGG CTGTCGTCTG CCATCAACCA CAGATTTGCA
    GGACAGATGG CGGCTCTGTG TTCCCGGGAT GACTTCCTCG GCAGCTTCTG TCGCTACCAC
    CTCACAGAAC CTGGGCTGGC CAGCAGGCAC CTGCTGAGCC CTGTGGGGCG GAGGCAGGTG
    GCCGGCCACA CCCGCGGCCC CAGGCTCAGC CTGCGCTTCC TGGGCAGTTA CCGGACGCTG
    GTCTCGCTGC TGCTGGCCTT CTTCGTGGCC TCTCTGTTCT GCGTCGGGCC CCTCCCATGC
    ACGCTGCTGC TCACCCTGGG CTATGTCCTC TACGCCTCTG CCATGACACT GCTGACCGAG
    CGGGGGAAGC TGCACCAGCC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016859937.1
    CDS866..2689
    Translation

    Target ORF information:

    RefSeq Version XM_017004448.2
    Organism Homo sapiens(human)
    Definition Homo sapiens sphingomyelin phosphodiesterase 4 (SMPD4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017004448.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCAGACCC CGCCTCCCAC GAGATCTGGA GGTCAGAAAC TCTGCTCCAG GTTTTTGTTG 
    AAATGTGGCT TCATCACTAT TCCTTGGAGA TGTATCAAAA AATGCAGTCC CCTCATGCCA
    AGCTGGAGGT TCTGCACTAC CGACTCAGTG TCTCCAGCGC CCTCTACAGC CCCGCCCAAC
    CCAGCCTCCA GGCCCTCCAC GCCTACCAAG TCCCTGGGCA ACAGAGGAAG TGACGCCCGG
    GGCTTCTACT GGGCCGAGTT CTCATCTGGC TGTGTCCAGG ATGAGGAGCA GGAGTCGTTC
    ACGCCTACTG AGGAGCATGT GTTGGTGGTG CGCCTGCTGC TGAAGCACCT GCACGCCTTT
    GCCAACAGCC TGAAGCCAGA GCAGGCCTCA CCCTCCGCCC ACTCCCACGC CACCAGCCCC
    CTGGAGGAGT TCAAACGGGC TGCTGTCCCG AGGTTCGTCC AGCAGAAACT CTACCTCTTC
    TTGCAGCATT GCTTTGGCCA CTGGCCCCTG GACGCATCGT TCAGAGCTGT CCTGGAGATG
    TGGCTGAGCT ACCTGCAGCC GTGGCGGTAC GCGCCTGACA AGCAGGCTCC GGGCAGCGAC
    TCCCAGCCCC GGTGTGTGTC GGAGAAATGG GCACCCTTTG TCCAGGAGAA CCTGCTGATG
    TACACCAAGT TGTTTGTGGG CTTTCTGAAC CGCGCGCTCC GCACAGACCT GGTCAGCCCC
    AAGCACGCGC TCATGGTGTT CCGAGTGGCC AAAGTCTTTG CCCAGCCCAA CCTGGCTGAG
    ATGATTCAGA AAGGTGAGCA GCTATTCCTG GAGCCAGAGC TGGTCATCCC CCACCGCCAG
    CACCGACTCT TCACGGCCCC CACATTCACT GGGAGCTTCC TGTCACCCTG GCCACCAGCG
    GTCACTGATG CCTCCTTCAA GGTGAAGAGC CACGTCTACA GCCTGGAGGG CCAGGACTGC
    AAGTACACCC CGATGTTTGG GCCCGAGGCC CGCACCCTGG TCCTGCGCCT CGCTCAGCTC
    ATCACACAGG CCAAACACAC AGCCAAGTCC ATCTCCGACC AGTGTGCGGA GAGCCCGGCT
    GGCCACTCCT TCCTCTCATG GCTGGGCTTT AGCTCCATGG ACACCAATGG CTCCTACACA
    GCCAACGACC TGGACGAGAT GGGGCAAGAC AGTGTCCGGA AGACAGATGA ATACCTGGAG
    AAGGCCCTGG AGTACCTGCG CCAGATATTC CGGCTCAGCG AAGCGCAGCT CAGGCAGTTC
    ACACTCGCCT TGGGCACCAC CCAGGATGAG AATGGAAAAA AGCAACTCCC CGACTGCATC
    GTGGGTGAGG ACGGACTCAT CCTTACGCCC CTGGGGCGGT ACCAGATCAT CAATGGGCTG
    CGAAGGTTTG AAATTGAGTA CCAGGGGGAC CCGGAGCTGC AGCCCATCCG GAGCTATGAG
    ATCGCCAGCT TGGTCCGCAC ACTCTTTAGG CTGTCGTCTG CCATCAACCA CAGATTTGCA
    GGACAGATGG CGGCTCTGTG TTCCCGGGAT GACTTCCTCG GCAGCTTCTG TCGCTACCAC
    CTCACAGAAC CTGGGCTGGC CAGCAGGCAC CTGCTGAGCC CTGTGGGGCG GAGGCAGGTG
    GCCGGCCACA CCCGCGGCCC CAGGCTCAGC CTGCGCTTCC TGGGCAGTTA CCGGACGCTG
    GTCTCGCTGC TGCTGGCCTT CTTCGTGGCC TCTCTGTTCT GCGTCGGGCC CCTCCCATGC
    ACGCTGCTGC TCACCCTGGG CTATGTCCTC TACGCCTCTG CCATGACACT GCTGACCGAG
    CGGGGGAAGC TGCACCAGCC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu89643
    Clone ID Related Accession (Same CDS sequence) XM_017004448.2 , XM_024452978.1 , XM_017004448.1
    Accession Version XM_024452978.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1824bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product sphingomyelin phosphodiesterase 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCAGACCC CGCCTCCCAC GAGATCTGGA GGTCAGAAAC TCTGCTCCAG GTTTTTGTTG 
    AAATGTGGCT TCATCACTAT TCCTTGGAGA TGTATCAAAA AATGCAGTCC CCTCATGCCA
    AGCTGGAGGT TCTGCACTAC CGACTCAGTG TCTCCAGCGC CCTCTACAGC CCCGCCCAAC
    CCAGCCTCCA GGCCCTCCAC GCCTACCAAG TCCCTGGGCA ACAGAGGAAG TGACGCCCGG
    GGCTTCTACT GGGCCGAGTT CTCATCTGGC TGTGTCCAGG ATGAGGAGCA GGAGTCGTTC
    ACGCCTACTG AGGAGCATGT GTTGGTGGTG CGCCTGCTGC TGAAGCACCT GCACGCCTTT
    GCCAACAGCC TGAAGCCAGA GCAGGCCTCA CCCTCCGCCC ACTCCCACGC CACCAGCCCC
    CTGGAGGAGT TCAAACGGGC TGCTGTCCCG AGGTTCGTCC AGCAGAAACT CTACCTCTTC
    TTGCAGCATT GCTTTGGCCA CTGGCCCCTG GACGCATCGT TCAGAGCTGT CCTGGAGATG
    TGGCTGAGCT ACCTGCAGCC GTGGCGGTAC GCGCCTGACA AGCAGGCTCC GGGCAGCGAC
    TCCCAGCCCC GGTGTGTGTC GGAGAAATGG GCACCCTTTG TCCAGGAGAA CCTGCTGATG
    TACACCAAGT TGTTTGTGGG CTTTCTGAAC CGCGCGCTCC GCACAGACCT GGTCAGCCCC
    AAGCACGCGC TCATGGTGTT CCGAGTGGCC AAAGTCTTTG CCCAGCCCAA CCTGGCTGAG
    ATGATTCAGA AAGGTGAGCA GCTATTCCTG GAGCCAGAGC TGGTCATCCC CCACCGCCAG
    CACCGACTCT TCACGGCCCC CACATTCACT GGGAGCTTCC TGTCACCCTG GCCACCAGCG
    GTCACTGATG CCTCCTTCAA GGTGAAGAGC CACGTCTACA GCCTGGAGGG CCAGGACTGC
    AAGTACACCC CGATGTTTGG GCCCGAGGCC CGCACCCTGG TCCTGCGCCT CGCTCAGCTC
    ATCACACAGG CCAAACACAC AGCCAAGTCC ATCTCCGACC AGTGTGCGGA GAGCCCGGCT
    GGCCACTCCT TCCTCTCATG GCTGGGCTTT AGCTCCATGG ACACCAATGG CTCCTACACA
    GCCAACGACC TGGACGAGAT GGGGCAAGAC AGTGTCCGGA AGACAGATGA ATACCTGGAG
    AAGGCCCTGG AGTACCTGCG CCAGATATTC CGGCTCAGCG AAGCGCAGCT CAGGCAGTTC
    ACACTCGCCT TGGGCACCAC CCAGGATGAG AATGGAAAAA AGCAACTCCC CGACTGCATC
    GTGGGTGAGG ACGGACTCAT CCTTACGCCC CTGGGGCGGT ACCAGATCAT CAATGGGCTG
    CGAAGGTTTG AAATTGAGTA CCAGGGGGAC CCGGAGCTGC AGCCCATCCG GAGCTATGAG
    ATCGCCAGCT TGGTCCGCAC ACTCTTTAGG CTGTCGTCTG CCATCAACCA CAGATTTGCA
    GGACAGATGG CGGCTCTGTG TTCCCGGGAT GACTTCCTCG GCAGCTTCTG TCGCTACCAC
    CTCACAGAAC CTGGGCTGGC CAGCAGGCAC CTGCTGAGCC CTGTGGGGCG GAGGCAGGTG
    GCCGGCCACA CCCGCGGCCC CAGGCTCAGC CTGCGCTTCC TGGGCAGTTA CCGGACGCTG
    GTCTCGCTGC TGCTGGCCTT CTTCGTGGCC TCTCTGTTCT GCGTCGGGCC CCTCCCATGC
    ACGCTGCTGC TCACCCTGGG CTATGTCCTC TACGCCTCTG CCATGACACT GCTGACCGAG
    CGGGGGAAGC TGCACCAGCC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024308746.1
    CDS1088..2911
    Translation

    Target ORF information:

    RefSeq Version XM_024452978.1
    Organism Homo sapiens(human)
    Definition Homo sapiens sphingomyelin phosphodiesterase 4 (SMPD4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024452978.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCAGACCC CGCCTCCCAC GAGATCTGGA GGTCAGAAAC TCTGCTCCAG GTTTTTGTTG 
    AAATGTGGCT TCATCACTAT TCCTTGGAGA TGTATCAAAA AATGCAGTCC CCTCATGCCA
    AGCTGGAGGT TCTGCACTAC CGACTCAGTG TCTCCAGCGC CCTCTACAGC CCCGCCCAAC
    CCAGCCTCCA GGCCCTCCAC GCCTACCAAG TCCCTGGGCA ACAGAGGAAG TGACGCCCGG
    GGCTTCTACT GGGCCGAGTT CTCATCTGGC TGTGTCCAGG ATGAGGAGCA GGAGTCGTTC
    ACGCCTACTG AGGAGCATGT GTTGGTGGTG CGCCTGCTGC TGAAGCACCT GCACGCCTTT
    GCCAACAGCC TGAAGCCAGA GCAGGCCTCA CCCTCCGCCC ACTCCCACGC CACCAGCCCC
    CTGGAGGAGT TCAAACGGGC TGCTGTCCCG AGGTTCGTCC AGCAGAAACT CTACCTCTTC
    TTGCAGCATT GCTTTGGCCA CTGGCCCCTG GACGCATCGT TCAGAGCTGT CCTGGAGATG
    TGGCTGAGCT ACCTGCAGCC GTGGCGGTAC GCGCCTGACA AGCAGGCTCC GGGCAGCGAC
    TCCCAGCCCC GGTGTGTGTC GGAGAAATGG GCACCCTTTG TCCAGGAGAA CCTGCTGATG
    TACACCAAGT TGTTTGTGGG CTTTCTGAAC CGCGCGCTCC GCACAGACCT GGTCAGCCCC
    AAGCACGCGC TCATGGTGTT CCGAGTGGCC AAAGTCTTTG CCCAGCCCAA CCTGGCTGAG
    ATGATTCAGA AAGGTGAGCA GCTATTCCTG GAGCCAGAGC TGGTCATCCC CCACCGCCAG
    CACCGACTCT TCACGGCCCC CACATTCACT GGGAGCTTCC TGTCACCCTG GCCACCAGCG
    GTCACTGATG CCTCCTTCAA GGTGAAGAGC CACGTCTACA GCCTGGAGGG CCAGGACTGC
    AAGTACACCC CGATGTTTGG GCCCGAGGCC CGCACCCTGG TCCTGCGCCT CGCTCAGCTC
    ATCACACAGG CCAAACACAC AGCCAAGTCC ATCTCCGACC AGTGTGCGGA GAGCCCGGCT
    GGCCACTCCT TCCTCTCATG GCTGGGCTTT AGCTCCATGG ACACCAATGG CTCCTACACA
    GCCAACGACC TGGACGAGAT GGGGCAAGAC AGTGTCCGGA AGACAGATGA ATACCTGGAG
    AAGGCCCTGG AGTACCTGCG CCAGATATTC CGGCTCAGCG AAGCGCAGCT CAGGCAGTTC
    ACACTCGCCT TGGGCACCAC CCAGGATGAG AATGGAAAAA AGCAACTCCC CGACTGCATC
    GTGGGTGAGG ACGGACTCAT CCTTACGCCC CTGGGGCGGT ACCAGATCAT CAATGGGCTG
    CGAAGGTTTG AAATTGAGTA CCAGGGGGAC CCGGAGCTGC AGCCCATCCG GAGCTATGAG
    ATCGCCAGCT TGGTCCGCAC ACTCTTTAGG CTGTCGTCTG CCATCAACCA CAGATTTGCA
    GGACAGATGG CGGCTCTGTG TTCCCGGGAT GACTTCCTCG GCAGCTTCTG TCGCTACCAC
    CTCACAGAAC CTGGGCTGGC CAGCAGGCAC CTGCTGAGCC CTGTGGGGCG GAGGCAGGTG
    GCCGGCCACA CCCGCGGCCC CAGGCTCAGC CTGCGCTTCC TGGGCAGTTA CCGGACGCTG
    GTCTCGCTGC TGCTGGCCTT CTTCGTGGCC TCTCTGTTCT GCGTCGGGCC CCTCCCATGC
    ACGCTGCTGC TCACCCTGGG CTATGTCCTC TACGCCTCTG CCATGACACT GCTGACCGAG
    CGGGGGAAGC TGCACCAGCC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu89643
    Clone ID Related Accession (Same CDS sequence) XM_017004448.2 , XM_024452978.1 , XM_017004448.1
    Accession Version XM_017004448.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1824bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product sphingomyelin phosphodiesterase 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCAGACCC CGCCTCCCAC GAGATCTGGA GGTCAGAAAC TCTGCTCCAG GTTTTTGTTG 
    AAATGTGGCT TCATCACTAT TCCTTGGAGA TGTATCAAAA AATGCAGTCC CCTCATGCCA
    AGCTGGAGGT TCTGCACTAC CGACTCAGTG TCTCCAGCGC CCTCTACAGC CCCGCCCAAC
    CCAGCCTCCA GGCCCTCCAC GCCTACCAAG TCCCTGGGCA ACAGAGGAAG TGACGCCCGG
    GGCTTCTACT GGGCCGAGTT CTCATCTGGC TGTGTCCAGG ATGAGGAGCA GGAGTCGTTC
    ACGCCTACTG AGGAGCATGT GTTGGTGGTG CGCCTGCTGC TGAAGCACCT GCACGCCTTT
    GCCAACAGCC TGAAGCCAGA GCAGGCCTCA CCCTCCGCCC ACTCCCACGC CACCAGCCCC
    CTGGAGGAGT TCAAACGGGC TGCTGTCCCG AGGTTCGTCC AGCAGAAACT CTACCTCTTC
    TTGCAGCATT GCTTTGGCCA CTGGCCCCTG GACGCATCGT TCAGAGCTGT CCTGGAGATG
    TGGCTGAGCT ACCTGCAGCC GTGGCGGTAC GCGCCTGACA AGCAGGCTCC GGGCAGCGAC
    TCCCAGCCCC GGTGTGTGTC GGAGAAATGG GCACCCTTTG TCCAGGAGAA CCTGCTGATG
    TACACCAAGT TGTTTGTGGG CTTTCTGAAC CGCGCGCTCC GCACAGACCT GGTCAGCCCC
    AAGCACGCGC TCATGGTGTT CCGAGTGGCC AAAGTCTTTG CCCAGCCCAA CCTGGCTGAG
    ATGATTCAGA AAGGTGAGCA GCTATTCCTG GAGCCAGAGC TGGTCATCCC CCACCGCCAG
    CACCGACTCT TCACGGCCCC CACATTCACT GGGAGCTTCC TGTCACCCTG GCCACCAGCG
    GTCACTGATG CCTCCTTCAA GGTGAAGAGC CACGTCTACA GCCTGGAGGG CCAGGACTGC
    AAGTACACCC CGATGTTTGG GCCCGAGGCC CGCACCCTGG TCCTGCGCCT CGCTCAGCTC
    ATCACACAGG CCAAACACAC AGCCAAGTCC ATCTCCGACC AGTGTGCGGA GAGCCCGGCT
    GGCCACTCCT TCCTCTCATG GCTGGGCTTT AGCTCCATGG ACACCAATGG CTCCTACACA
    GCCAACGACC TGGACGAGAT GGGGCAAGAC AGTGTCCGGA AGACAGATGA ATACCTGGAG
    AAGGCCCTGG AGTACCTGCG CCAGATATTC CGGCTCAGCG AAGCGCAGCT CAGGCAGTTC
    ACACTCGCCT TGGGCACCAC CCAGGATGAG AATGGAAAAA AGCAACTCCC CGACTGCATC
    GTGGGTGAGG ACGGACTCAT CCTTACGCCC CTGGGGCGGT ACCAGATCAT CAATGGGCTG
    CGAAGGTTTG AAATTGAGTA CCAGGGGGAC CCGGAGCTGC AGCCCATCCG GAGCTATGAG
    ATCGCCAGCT TGGTCCGCAC ACTCTTTAGG CTGTCGTCTG CCATCAACCA CAGATTTGCA
    GGACAGATGG CGGCTCTGTG TTCCCGGGAT GACTTCCTCG GCAGCTTCTG TCGCTACCAC
    CTCACAGAAC CTGGGCTGGC CAGCAGGCAC CTGCTGAGCC CTGTGGGGCG GAGGCAGGTG
    GCCGGCCACA CCCGCGGCCC CAGGCTCAGC CTGCGCTTCC TGGGCAGTTA CCGGACGCTG
    GTCTCGCTGC TGCTGGCCTT CTTCGTGGCC TCTCTGTTCT GCGTCGGGCC CCTCCCATGC
    ACGCTGCTGC TCACCCTGGG CTATGTCCTC TACGCCTCTG CCATGACACT GCTGACCGAG
    CGGGGGAAGC TGCACCAGCC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016859937.1
    CDS918..2741
    Translation

    Target ORF information:

    RefSeq Version XM_017004448.1
    Organism Homo sapiens(human)
    Definition Homo sapiens sphingomyelin phosphodiesterase 4 (SMPD4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017004448.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCAGACCC CGCCTCCCAC GAGATCTGGA GGTCAGAAAC TCTGCTCCAG GTTTTTGTTG 
    AAATGTGGCT TCATCACTAT TCCTTGGAGA TGTATCAAAA AATGCAGTCC CCTCATGCCA
    AGCTGGAGGT TCTGCACTAC CGACTCAGTG TCTCCAGCGC CCTCTACAGC CCCGCCCAAC
    CCAGCCTCCA GGCCCTCCAC GCCTACCAAG TCCCTGGGCA ACAGAGGAAG TGACGCCCGG
    GGCTTCTACT GGGCCGAGTT CTCATCTGGC TGTGTCCAGG ATGAGGAGCA GGAGTCGTTC
    ACGCCTACTG AGGAGCATGT GTTGGTGGTG CGCCTGCTGC TGAAGCACCT GCACGCCTTT
    GCCAACAGCC TGAAGCCAGA GCAGGCCTCA CCCTCCGCCC ACTCCCACGC CACCAGCCCC
    CTGGAGGAGT TCAAACGGGC TGCTGTCCCG AGGTTCGTCC AGCAGAAACT CTACCTCTTC
    TTGCAGCATT GCTTTGGCCA CTGGCCCCTG GACGCATCGT TCAGAGCTGT CCTGGAGATG
    TGGCTGAGCT ACCTGCAGCC GTGGCGGTAC GCGCCTGACA AGCAGGCTCC GGGCAGCGAC
    TCCCAGCCCC GGTGTGTGTC GGAGAAATGG GCACCCTTTG TCCAGGAGAA CCTGCTGATG
    TACACCAAGT TGTTTGTGGG CTTTCTGAAC CGCGCGCTCC GCACAGACCT GGTCAGCCCC
    AAGCACGCGC TCATGGTGTT CCGAGTGGCC AAAGTCTTTG CCCAGCCCAA CCTGGCTGAG
    ATGATTCAGA AAGGTGAGCA GCTATTCCTG GAGCCAGAGC TGGTCATCCC CCACCGCCAG
    CACCGACTCT TCACGGCCCC CACATTCACT GGGAGCTTCC TGTCACCCTG GCCACCAGCG
    GTCACTGATG CCTCCTTCAA GGTGAAGAGC CACGTCTACA GCCTGGAGGG CCAGGACTGC
    AAGTACACCC CGATGTTTGG GCCCGAGGCC CGCACCCTGG TCCTGCGCCT CGCTCAGCTC
    ATCACACAGG CCAAACACAC AGCCAAGTCC ATCTCCGACC AGTGTGCGGA GAGCCCGGCT
    GGCCACTCCT TCCTCTCATG GCTGGGCTTT AGCTCCATGG ACACCAATGG CTCCTACACA
    GCCAACGACC TGGACGAGAT GGGGCAAGAC AGTGTCCGGA AGACAGATGA ATACCTGGAG
    AAGGCCCTGG AGTACCTGCG CCAGATATTC CGGCTCAGCG AAGCGCAGCT CAGGCAGTTC
    ACACTCGCCT TGGGCACCAC CCAGGATGAG AATGGAAAAA AGCAACTCCC CGACTGCATC
    GTGGGTGAGG ACGGACTCAT CCTTACGCCC CTGGGGCGGT ACCAGATCAT CAATGGGCTG
    CGAAGGTTTG AAATTGAGTA CCAGGGGGAC CCGGAGCTGC AGCCCATCCG GAGCTATGAG
    ATCGCCAGCT TGGTCCGCAC ACTCTTTAGG CTGTCGTCTG CCATCAACCA CAGATTTGCA
    GGACAGATGG CGGCTCTGTG TTCCCGGGAT GACTTCCTCG GCAGCTTCTG TCGCTACCAC
    CTCACAGAAC CTGGGCTGGC CAGCAGGCAC CTGCTGAGCC CTGTGGGGCG GAGGCAGGTG
    GCCGGCCACA CCCGCGGCCC CAGGCTCAGC CTGCGCTTCC TGGGCAGTTA CCGGACGCTG
    GTCTCGCTGC TGCTGGCCTT CTTCGTGGCC TCTCTGTTCT GCGTCGGGCC CCTCCCATGC
    ACGCTGCTGC TCACCCTGGG CTATGTCCTC TACGCCTCTG CCATGACACT GCTGACCGAG
    CGGGGGAAGC TGCACCAGCC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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