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NDC1 cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol NDC1
Entrez Gene ID 55706
Full Name NDC1 transmembrane nucleoporin
Synonyms NET3,TMEM48
General protein information
Preferred Names

NDC1 transmembrane nucleoporin

Names

nucleoporin NDC1
nuclear division cycle 1 homolog
transmembrane protein 48
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

1

1p32.3
Disorder MIM:

610115


Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_018087.4 NP_060557.3 nucleoporin NDC1 isoform 1
NM_001168551.1 NP_001162023.1 nucleoporin NDC1 isoform 2
NM_001168551.2 NP_001162023.1 nucleoporin NDC1 isoform 2
XM_011541766.2 XP_011540068.1 nucleoporin NDC1 isoform X1
NM_018087.5 NP_060557.3 nucleoporin NDC1 isoform 1

Pathways from BioSystems


Homologies


Gallus gallus (chicken) TMEM48 XP_001235442.3
Pan troglodytes (chimpanzee) TMEM48 XP_513417.4
Drosophila melanogaster (fruit fly) CG5857 NP_651191.1
Homo sapiens (human) NDC1 NP_060557.3
Anopheles gambiae (African malaria mosquito) AgaP_AGAP004558 XP_313856.5
Xenopus tropicalis (tropical clawed frog) ndc1 NP_001016813.1
Canis lupus familiaris (dog) NDC1 XP_003434706.1
Bos taurus (cattle) NDC1 XP_003585949.2
Mus musculus (house mouse) Ndc1 NP_082631.1
Rattus norvegicus (Norway rat) Ndc1 NP_001020194.1
Danio rerio (zebrafish) ndc1 NP_956237.1

Gene Ontology


Bibliography

Related articles in PubMed

SEPT12-NDC1 Complexes Are Required for Mammalian Spermiogenesis.
Lai TH, Wu YY, Wang YY, Chen MF, Wang P, Chen TM, Wu YN, Chiang HS, Kuo PL, Lin YH
International journal of molecular sciences17(11)(2016 Nov)

Overexpression and biological function of TMEM48 in non-small cell lung carcinoma.
Qiao W, Han Y, Jin W, Tian M, Chen P, Min J, Hu H, Xu B, Zhu W, Xiong L, Lin Q
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine37(2)2575-86(2016 Feb)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

The nuclear pore complex protein ALADIN is anchored via NDC1 but not via POM121 and GP210 in the nuclear envelope.
Kind B, Koehler K, Lorenz M, Huebner A
Biochemical and biophysical research communications390(2)205-10(2009 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In mature human spermatozoa, SEPT12 and NDC1 are majorly colocalized in the centrosome regions; however, NDC1 is only slightly co-expressed with SEPT12 at the annulus of the sperm tail.
Title: SEPT12-NDC1 Complexes Are Required for Mammalian Spermiogenesis.

The biological relevance of TMEM48 in NSCLC progression.
Title: Overexpression and biological function of TMEM48 in non-small cell lung carcinoma.

ALADIN is anchored in the nuclear envelope via NDC1 and that this interaction gets lost, if ALADIN is mutated.
Title: The nuclear pore complex protein ALADIN is anchored via NDC1 but not via POM121 and GP210 in the nuclear envelope.

NDC1-mediated localization of ALADIN to nuclear pore complexes is essential for selective nuclear protein import; abrogation of the interaction between ALADIN and NDC1 may be important for the development of triple-A syndrome.
Title: The transmembrane nucleoporin NDC1 is required for targeting of ALADIN to nuclear pore complexes.

Human homologue NDC1/NET3 contains three FG repeats in the C-terminus, a feature of many nuclear pore proteins.
Title: Topology of yeast Ndc1p: predictions for the human NDC1/NET3 homologue.

The following NDC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NDC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu01464 NM_018087.5
Latest version!		 Homo sapiens NDC1 transmembrane nucleoporin (NDC1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$49.50-$69.30
$99.00
OHu32206 NM_001168551.2
Latest version!		 Homo sapiens NDC1 transmembrane nucleoporin (NDC1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $69.30
$99.00
OHu69653 XM_011541766.2
Latest version!		 Homo sapiens NDC1 transmembrane nucleoporin (NDC1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
View Old Accession Versions >>
OHu32206 NM_001168551.1 Homo sapiens NDC1 transmembrane nucleoporin (NDC1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $69.30
$99.00
OHu01464 NM_018087.4 Homo sapiens NDC1 transmembrane nucleoporin (NDC1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$49.50-$69.30
$99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu01464
Clone ID Related Accession (Same CDS sequence) NM_018087.4 , NM_018087.5
Accession Version NM_018087.4 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2025bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-10-01
Organism Homo sapiens(human)
Product nucleoporin NDC1 isoform 1
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC413103.1, BC003082.2, AL049745.9, AL354612.1 and BM554303.1. On Dec 11, 2009 this sequence version replaced NM_018087.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1660807.105919.1, SRR1803611.271718.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGCCACGG CCGTGAGCCG GCCCTGCGCC GGCAGGTCGC GGGACATACT GTGGCGCGTT 
TTGGGCTGGA GGATAGTTGC AAGTATTGTT TGGTCAGTGC TATTTCTACC CATCTGCACC 
ACAGTATTTA TAATTTTCAG CAGGATTGAT TTGTTTCATC CTATACAGTG GCTGTCTGAT 
TCTTTCAGTG ACCTGTATAG TTCCTATGTA ATCTTTTACT TCCTGCTGCT GTCAGTGGTA 
ATAATAATAA TAAGTATTTT CAATGTGGAG TTCTATGCAG TTGTGCCTTC TATTCCTTGC 
TCCAGACTAG CTCTGATAGG GAAGATCATT CATCCTCAGC AACTCATGCA CTCATTTATT 
CATGCTGCAA TGGGAATGGT GATGGCCTGG TGTGCTGCAG TGATAACCCA GGGCCAGTAC 
AGCTTTCTTG TGGTTCCCTG CACTGGTACT AACAGCTTTG GTAGCCCTGC TGCGCAAACC 
TGCTTAAATG AATATCATCT TTTTTTCCTA CTGACTGGAG CATTTATGGG CTATAGCTAT 
AGCCTCCTGT ATTTTGTTAA CAACATGAAC TATCTTCCAT TTCCCATCAT ACAGCAATAC 
AAGTTCTTGC GTTTTAGGAG ATCTCTGCTC TTATTAGTTA AACACAGTTG TGTGGAATCA 
CTGTTCCTGG TTAGAAATTT CTGCATTTTA TATTATTTTC TTGGCTATAT TCCCAAAGCT 
TGGATTAGCA CTGCTATGAA CCTTCACATA GATGAGCAGG TTCATAGGCC ACTTGACACA 
GTGAGTGGCC TCTTAAATCT CTCGTTACTC TACCATGTCT GGCTGTGTGG TGTCTTTCTC 
CTGACGACTT GGTATGTCTC ATGGATACTC TTCAAAATCT ATGCCACAGA GGCTCATGTG 
TTTCCTGTTC AACCACCATT TGCAGAAGGG TCAGATGAGT GCCTTCCAAA AGTGTTAAAT 
AGCAATCCTC CCCCCATCAT AAAGTATTTA GCCTTGCAGG ACCTGATGTT GCTTTCTCAA 
TATTCTCCTT CACGAAGACA AGAAGTTTTC AGCCTCAGCC AACCAGGTGG ACATCCCCAC 
AATTGGACAG CCATTTCAAG GGAGTGTTTG AATCTTTTAA ATGGTATGAC TCAGAAACTG 
ATTCTCTATC AAGAAGCTGC TGCTACGAAT GGGAGAGTGT CTTCATCTTA CCCAGTGGAA 
CCTAAGAAAT TAAATTCTCC AGAAGAAACT GCTTTTCAGA CACCAAAATC TAGCCAGATG 
CCTCGGCCTT CAGTGCCACC ATTAGTTAAA ACATCACTGT TTTCTTCAAA ATTATCTACA 
CCTGATGTTG TGAGCCCATT TGGGACCCCA TTTGGCTCTA GTGTAATGAA TCGGATGGCT 
GGAATTTTTG ATGTAAACAC CTGCTATGGG TCACCGCAAA GTCCTCAGCT AATAAGAAGG 
GGGCCAAGAT TGTGGACATC AGCTTCTGAT CAGCAAATGA CTGAATTTTC TAATCCTTCT 
CCATCTACCT CTATTAGTGC TGAGGGTAAG ACAATGAGAC AACCCAGTGT GATTTATTCA 
TGGATTCAGA ATAAACGTGA ACAGATTAAG AATTTCTTGT CAAAACGGGT GCTGATAATG 
TATTTTTTCA GTAAGCACCC AGAGGCCTCC ATTCAGGCTG TTTTTTCAGA TGCCCAAATG 
CATATTTGGG CATTAGAAGG TCTGTCGCAC TTAGTAGCAG CATCATTTAC AGAGGATAGA 
TTTGGAGTTG TCCAGACGAC ACTACCAGCT ATCCTTAATA CTTTGTTGAC ACTGCAAGAG 
GCAGTCGACA AGTACTTTAA GCTTCCTCAT GCTTCCAGTA AACCACCCCG GATTTCAGGA 
AGCCTTGTGG ACACTTCATA TAAAACATTA AGATTTGCAT TCAGAGCATC ACTGAAAACT 
GCCATCTATC GAATAACTAC TACATTTGGT GAACATCTGA ATGCTGTGCA AGCATCTGCA 
GAACATCAGA AAAGACTTCA ACAGTTCTTG GAGTTCAAAG AATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_060557.3
CDS292..2316
Translation

Target ORF information:

RefSeq Version NM_018087.4
Organism Homo sapiens(human)
Definition Homo sapiens NDC1 transmembrane nucleoporin (NDC1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_018087.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGCCACGG CCGTGAGCCG GCCCTGCGCC GGCAGGTCGC GGGACATACT GTGGCGCGTT 
TTGGGCTGGA GGATAGTTGC AAGTATTGTT TGGTCAGTGC TATTTCTACC CATCTGCACC 
ACAGTATTTA TAATTTTCAG CAGGATTGAT TTGTTTCATC CTATACAGTG GCTGTCTGAT 
TCTTTCAGTG ACCTGTATAG TTCCTATGTA ATCTTTTACT TCCTGCTGCT GTCAGTGGTA 
ATAATAATAA TAAGTATTTT CAATGTGGAG TTCTATGCAG TTGTGCCTTC TATTCCTTGC 
TCCAGACTAG CTCTGATAGG GAAGATCATT CATCCTCAGC AACTCATGCA CTCATTTATT 
CATGCTGCAA TGGGAATGGT GATGGCCTGG TGTGCTGCAG TGATAACCCA GGGCCAGTAC 
AGCTTTCTTG TGGTTCCCTG CACTGGTACT AACAGCTTTG GTAGCCCTGC TGCGCAAACC 
TGCTTAAATG AATATCATCT TTTTTTCCTA CTGACTGGAG CATTTATGGG CTATAGCTAT 
AGCCTCCTGT ATTTTGTTAA CAACATGAAC TATCTTCCAT TTCCCATCAT ACAGCAATAC 
AAGTTCTTGC GTTTTAGGAG ATCTCTGCTC TTATTAGTTA AACACAGTTG TGTGGAATCA 
CTGTTCCTGG TTAGAAATTT CTGCATTTTA TATTATTTTC TTGGCTATAT TCCCAAAGCT 
TGGATTAGCA CTGCTATGAA CCTTCACATA GATGAGCAGG TTCATAGGCC ACTTGACACA 
GTGAGTGGCC TCTTAAATCT CTCGTTACTC TACCATGTCT GGCTGTGTGG TGTCTTTCTC 
CTGACGACTT GGTATGTCTC ATGGATACTC TTCAAAATCT ATGCCACAGA GGCTCATGTG 
TTTCCTGTTC AACCACCATT TGCAGAAGGG TCAGATGAGT GCCTTCCAAA AGTGTTAAAT 
AGCAATCCTC CCCCCATCAT AAAGTATTTA GCCTTGCAGG ACCTGATGTT GCTTTCTCAA 
TATTCTCCTT CACGAAGACA AGAAGTTTTC AGCCTCAGCC AACCAGGTGG ACATCCCCAC 
AATTGGACAG CCATTTCAAG GGAGTGTTTG AATCTTTTAA ATGGTATGAC TCAGAAACTG 
ATTCTCTATC AAGAAGCTGC TGCTACGAAT GGGAGAGTGT CTTCATCTTA CCCAGTGGAA 
CCTAAGAAAT TAAATTCTCC AGAAGAAACT GCTTTTCAGA CACCAAAATC TAGCCAGATG 
CCTCGGCCTT CAGTGCCACC ATTAGTTAAA ACATCACTGT TTTCTTCAAA ATTATCTACA 
CCTGATGTTG TGAGCCCATT TGGGACCCCA TTTGGCTCTA GTGTAATGAA TCGGATGGCT 
GGAATTTTTG ATGTAAACAC CTGCTATGGG TCACCGCAAA GTCCTCAGCT AATAAGAAGG 
GGGCCAAGAT TGTGGACATC AGCTTCTGAT CAGCAAATGA CTGAATTTTC TAATCCTTCT 
CCATCTACCT CTATTAGTGC TGAGGGTAAG ACAATGAGAC AACCCAGTGT GATTTATTCA 
TGGATTCAGA ATAAACGTGA ACAGATTAAG AATTTCTTGT CAAAACGGGT GCTGATAATG 
TATTTTTTCA GTAAGCACCC AGAGGCCTCC ATTCAGGCTG TTTTTTCAGA TGCCCAAATG 
CATATTTGGG CATTAGAAGG TCTGTCGCAC TTAGTAGCAG CATCATTTAC AGAGGATAGA 
TTTGGAGTTG TCCAGACGAC ACTACCAGCT ATCCTTAATA CTTTGTTGAC ACTGCAAGAG 
GCAGTCGACA AGTACTTTAA GCTTCCTCAT GCTTCCAGTA AACCACCCCG GATTTCAGGA 
AGCCTTGTGG ACACTTCATA TAAAACATTA AGATTTGCAT TCAGAGCATC ACTGAAAACT 
GCCATCTATC GAATAACTAC TACATTTGGT GAACATCTGA ATGCTGTGCA AGCATCTGCA 
GAACATCAGA AAAGACTTCA ACAGTTCTTG GAGTTCAAAG AATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu01464
Clone ID Related Accession (Same CDS sequence) NM_018087.4 , NM_018087.5
Accession Version NM_018087.5 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2025bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-31
Organism Homo sapiens(human)
Product nucleoporin NDC1 isoform 1
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC003082.2, AL049745.9 and AL354612.1. On Nov 24, 2018 this sequence version replaced NM_018087.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.33376.1, SRR1660803.94921.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000371429.4/ ENSP00000360483.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGCCACGG CCGTGAGCCG GCCCTGCGCC GGCAGGTCGC GGGACATACT GTGGCGCGTT 
TTGGGCTGGA GGATAGTTGC AAGTATTGTT TGGTCAGTGC TATTTCTACC CATCTGCACC 
ACAGTATTTA TAATTTTCAG CAGGATTGAT TTGTTTCATC CTATACAGTG GCTGTCTGAT 
TCTTTCAGTG ACCTGTATAG TTCCTATGTA ATCTTTTACT TCCTGCTGCT GTCAGTGGTA 
ATAATAATAA TAAGTATTTT CAATGTGGAG TTCTATGCAG TTGTGCCTTC TATTCCTTGC 
TCCAGACTAG CTCTGATAGG GAAGATCATT CATCCTCAGC AACTCATGCA CTCATTTATT 
CATGCTGCAA TGGGAATGGT GATGGCCTGG TGTGCTGCAG TGATAACCCA GGGCCAGTAC 
AGCTTTCTTG TGGTTCCCTG CACTGGTACT AACAGCTTTG GTAGCCCTGC TGCGCAAACC 
TGCTTAAATG AATATCATCT TTTTTTCCTA CTGACTGGAG CATTTATGGG CTATAGCTAT 
AGCCTCCTGT ATTTTGTTAA CAACATGAAC TATCTTCCAT TTCCCATCAT ACAGCAATAC 
AAGTTCTTGC GTTTTAGGAG ATCTCTGCTC TTATTAGTTA AACACAGTTG TGTGGAATCA 
CTGTTCCTGG TTAGAAATTT CTGCATTTTA TATTATTTTC TTGGCTATAT TCCCAAAGCT 
TGGATTAGCA CTGCTATGAA CCTTCACATA GATGAGCAGG TTCATAGGCC ACTTGACACA 
GTGAGTGGCC TCTTAAATCT CTCGTTACTC TACCATGTCT GGCTGTGTGG TGTCTTTCTC 
CTGACGACTT GGTATGTCTC ATGGATACTC TTCAAAATCT ATGCCACAGA GGCTCATGTG 
TTTCCTGTTC AACCACCATT TGCAGAAGGG TCAGATGAGT GCCTTCCAAA AGTGTTAAAT 
AGCAATCCTC CCCCCATCAT AAAGTATTTA GCCTTGCAGG ACCTGATGTT GCTTTCTCAA 
TATTCTCCTT CACGAAGACA AGAAGTTTTC AGCCTCAGCC AACCAGGTGG ACATCCCCAC 
AATTGGACAG CCATTTCAAG GGAGTGTTTG AATCTTTTAA ATGGTATGAC TCAGAAACTG 
ATTCTCTATC AAGAAGCTGC TGCTACGAAT GGGAGAGTGT CTTCATCTTA CCCAGTGGAA 
CCTAAGAAAT TAAATTCTCC AGAAGAAACT GCTTTTCAGA CACCAAAATC TAGCCAGATG 
CCTCGGCCTT CAGTGCCACC ATTAGTTAAA ACATCACTGT TTTCTTCAAA ATTATCTACA 
CCTGATGTTG TGAGCCCATT TGGGACCCCA TTTGGCTCTA GTGTAATGAA TCGGATGGCT 
GGAATTTTTG ATGTAAACAC CTGCTATGGG TCACCGCAAA GTCCTCAGCT AATAAGAAGG 
GGGCCAAGAT TGTGGACATC AGCTTCTGAT CAGCAAATGA CTGAATTTTC TAATCCTTCT 
CCATCTACCT CTATTAGTGC TGAGGGTAAG ACAATGAGAC AACCCAGTGT GATTTATTCA 
TGGATTCAGA ATAAACGTGA ACAGATTAAG AATTTCTTGT CAAAACGGGT GCTGATAATG 
TATTTTTTCA GTAAGCACCC AGAGGCCTCC ATTCAGGCTG TTTTTTCAGA TGCCCAAATG 
CATATTTGGG CATTAGAAGG TCTGTCGCAC TTAGTAGCAG CATCATTTAC AGAGGATAGA 
TTTGGAGTTG TCCAGACGAC ACTACCAGCT ATCCTTAATA CTTTGTTGAC ACTGCAAGAG 
GCAGTCGACA AGTACTTTAA GCTTCCTCAT GCTTCCAGTA AACCACCCCG GATTTCAGGA 
AGCCTTGTGG ACACTTCATA TAAAACATTA AGATTTGCAT TCAGAGCATC ACTGAAAACT 
GCCATCTATC GAATAACTAC TACATTTGGT GAACATCTGA ATGCTGTGCA AGCATCTGCA 
GAACATCAGA AAAGACTTCA ACAGTTCTTG GAGTTCAAAG AATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_060557.3
CDS36..2060
Translation

Target ORF information:

RefSeq Version NM_018087.5
Organism Homo sapiens(human)
Definition Homo sapiens NDC1 transmembrane nucleoporin (NDC1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_018087.5

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGCCACGG CCGTGAGCCG GCCCTGCGCC GGCAGGTCGC GGGACATACT GTGGCGCGTT 
TTGGGCTGGA GGATAGTTGC AAGTATTGTT TGGTCAGTGC TATTTCTACC CATCTGCACC 
ACAGTATTTA TAATTTTCAG CAGGATTGAT TTGTTTCATC CTATACAGTG GCTGTCTGAT 
TCTTTCAGTG ACCTGTATAG TTCCTATGTA ATCTTTTACT TCCTGCTGCT GTCAGTGGTA 
ATAATAATAA TAAGTATTTT CAATGTGGAG TTCTATGCAG TTGTGCCTTC TATTCCTTGC 
TCCAGACTAG CTCTGATAGG GAAGATCATT CATCCTCAGC AACTCATGCA CTCATTTATT 
CATGCTGCAA TGGGAATGGT GATGGCCTGG TGTGCTGCAG TGATAACCCA GGGCCAGTAC 
AGCTTTCTTG TGGTTCCCTG CACTGGTACT AACAGCTTTG GTAGCCCTGC TGCGCAAACC 
TGCTTAAATG AATATCATCT TTTTTTCCTA CTGACTGGAG CATTTATGGG CTATAGCTAT 
AGCCTCCTGT ATTTTGTTAA CAACATGAAC TATCTTCCAT TTCCCATCAT ACAGCAATAC 
AAGTTCTTGC GTTTTAGGAG ATCTCTGCTC TTATTAGTTA AACACAGTTG TGTGGAATCA 
CTGTTCCTGG TTAGAAATTT CTGCATTTTA TATTATTTTC TTGGCTATAT TCCCAAAGCT 
TGGATTAGCA CTGCTATGAA CCTTCACATA GATGAGCAGG TTCATAGGCC ACTTGACACA 
GTGAGTGGCC TCTTAAATCT CTCGTTACTC TACCATGTCT GGCTGTGTGG TGTCTTTCTC 
CTGACGACTT GGTATGTCTC ATGGATACTC TTCAAAATCT ATGCCACAGA GGCTCATGTG 
TTTCCTGTTC AACCACCATT TGCAGAAGGG TCAGATGAGT GCCTTCCAAA AGTGTTAAAT 
AGCAATCCTC CCCCCATCAT AAAGTATTTA GCCTTGCAGG ACCTGATGTT GCTTTCTCAA 
TATTCTCCTT CACGAAGACA AGAAGTTTTC AGCCTCAGCC AACCAGGTGG ACATCCCCAC 
AATTGGACAG CCATTTCAAG GGAGTGTTTG AATCTTTTAA ATGGTATGAC TCAGAAACTG 
ATTCTCTATC AAGAAGCTGC TGCTACGAAT GGGAGAGTGT CTTCATCTTA CCCAGTGGAA 
CCTAAGAAAT TAAATTCTCC AGAAGAAACT GCTTTTCAGA CACCAAAATC TAGCCAGATG 
CCTCGGCCTT CAGTGCCACC ATTAGTTAAA ACATCACTGT TTTCTTCAAA ATTATCTACA 
CCTGATGTTG TGAGCCCATT TGGGACCCCA TTTGGCTCTA GTGTAATGAA TCGGATGGCT 
GGAATTTTTG ATGTAAACAC CTGCTATGGG TCACCGCAAA GTCCTCAGCT AATAAGAAGG 
GGGCCAAGAT TGTGGACATC AGCTTCTGAT CAGCAAATGA CTGAATTTTC TAATCCTTCT 
CCATCTACCT CTATTAGTGC TGAGGGTAAG ACAATGAGAC AACCCAGTGT GATTTATTCA 
TGGATTCAGA ATAAACGTGA ACAGATTAAG AATTTCTTGT CAAAACGGGT GCTGATAATG 
TATTTTTTCA GTAAGCACCC AGAGGCCTCC ATTCAGGCTG TTTTTTCAGA TGCCCAAATG 
CATATTTGGG CATTAGAAGG TCTGTCGCAC TTAGTAGCAG CATCATTTAC AGAGGATAGA 
TTTGGAGTTG TCCAGACGAC ACTACCAGCT ATCCTTAATA CTTTGTTGAC ACTGCAAGAG 
GCAGTCGACA AGTACTTTAA GCTTCCTCAT GCTTCCAGTA AACCACCCCG GATTTCAGGA 
AGCCTTGTGG ACACTTCATA TAAAACATTA AGATTTGCAT TCAGAGCATC ACTGAAAACT 
GCCATCTATC GAATAACTAC TACATTTGGT GAACATCTGA ATGCTGTGCA AGCATCTGCA 
GAACATCAGA AAAGACTTCA ACAGTTCTTG GAGTTCAAAG AATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu32206
Clone ID Related Accession (Same CDS sequence) NM_001168551.1 , NM_001168551.2
Accession Version NM_001168551.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1905bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-12-22
Organism Homo sapiens(human)
Product nucleoporin NDC1 isoform 2
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC413103.1, AK295000.1, BC003082.2, AL354612.1 and BM554303.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.173212.1, SRR1803615.269164.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGGCCACGG CCGTGAGCCG GCCCTGCGCC GGCAGGTCGC GGGACATACT GTGGCGCGTT 
TTGGGCTGGA GGATAGTTGC AAGTATTGTT TGGTCAGTGC TATTTCTACC CATCTGCACC 
ACAGTATTTA TAATTTTCAG CAGGATTGAT TTGTTTCATC CTATACAGTG GCTGTCTGAT 
TCTTTCAGTG ACCTGTATAG TTCCTATGTA ATCTTTTACT TCCTGCTGCT GTCAGTGGTA 
ATAATAATAA TAAGTATTTT CAATGTGGAG TTCTATGCAG TGATAACCCA GGGCCAGTAC 
AGCTTTCTTG TGGTTCCCTG CACTGGTACT AACAGCTTTG GTAGCCCTGC TGCGCAAACC 
TGCTTAAATG AATATCATCT TTTTTTCCTA CTGACTGGAG CATTTATGGG CTATAGCTAT 
AGCCTCCTGT ATTTTGTTAA CAACATGAAC TATCTTCCAT TTCCCATCAT ACAGCAATAC 
AAGTTCTTGC GTTTTAGGAG ATCTCTGCTC TTATTAGTTA AACACAGTTG TGTGGAATCA 
CTGTTCCTGG TTAGAAATTT CTGCATTTTA TATTATTTTC TTGGCTATAT TCCCAAAGCT 
TGGATTAGCA CTGCTATGAA CCTTCACATA GATGAGCAGG TTCATAGGCC ACTTGACACA 
GTGAGTGGCC TCTTAAATCT CTCGTTACTC TACCATGTCT GGCTGTGTGG TGTCTTTCTC 
CTGACGACTT GGTATGTCTC ATGGATACTC TTCAAAATCT ATGCCACAGA GGCTCATGTG 
TTTCCTGTTC AACCACCATT TGCAGAAGGG TCAGATGAGT GCCTTCCAAA AGTGTTAAAT 
AGCAATCCTC CCCCCATCAT AAAGTATTTA GCCTTGCAGG ACCTGATGTT GCTTTCTCAA 
TATTCTCCTT CACGAAGACA AGAAGTTTTC AGCCTCAGCC AACCAGGTGG ACATCCCCAC 
AATTGGACAG CCATTTCAAG GGAGTGTTTG AATCTTTTAA ATGGTATGAC TCAGAAACTG 
ATTCTCTATC AAGAAGCTGC TGCTACGAAT GGGAGAGTGT CTTCATCTTA CCCAGTGGAA 
CCTAAGAAAT TAAATTCTCC AGAAGAAACT GCTTTTCAGA CACCAAAATC TAGCCAGATG 
CCTCGGCCTT CAGTGCCACC ATTAGTTAAA ACATCACTGT TTTCTTCAAA ATTATCTACA 
CCTGATGTTG TGAGCCCATT TGGGACCCCA TTTGGCTCTA GTGTAATGAA TCGGATGGCT 
GGAATTTTTG ATGTAAACAC CTGCTATGGG TCACCGCAAA GTCCTCAGCT AATAAGAAGG 
GGGCCAAGAT TGTGGACATC AGCTTCTGAT CAGCAAATGA CTGAATTTTC TAATCCTTCT 
CCATCTACCT CTATTAGTGC TGAGGGTAAG ACAATGAGAC AACCCAGTGT GATTTATTCA 
TGGATTCAGA ATAAACGTGA ACAGATTAAG AATTTCTTGT CAAAACGGGT GCTGATAATG 
TATTTTTTCA GTAAGCACCC AGAGGCCTCC ATTCAGGCTG TTTTTTCAGA TGCCCAAATG 
CATATTTGGG CATTAGAAGG TCTGTCGCAC TTAGTAGCAG CATCATTTAC AGAGGATAGA 
TTTGGAGTTG TCCAGACGAC ACTACCAGCT ATCCTTAATA CTTTGTTGAC ACTGCAAGAG 
GCAGTCGACA AGTACTTTAA GCTTCCTCAT GCTTCCAGTA AACCACCCCG GATTTCAGGA 
AGCCTTGTGG ACACTTCATA TAAAACATTA AGATTTGCAT TCAGAGCATC ACTGAAAACT 
GCCATCTATC GAATAACTAC TACATTTGGT GAACATCTGA ATGCTGTGCA AGCATCTGCA 
GAACATCAGA AAAGACTTCA ACAGTTCTTG GAGTTCAAAG AATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001162023.1
CDS292..2196
Translation

Target ORF information:

RefSeq Version NM_001168551.1
Organism Homo sapiens(human)
Definition Homo sapiens NDC1 transmembrane nucleoporin (NDC1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001168551.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGGCCACGG CCGTGAGCCG GCCCTGCGCC GGCAGGTCGC GGGACATACT GTGGCGCGTT 
TTGGGCTGGA GGATAGTTGC AAGTATTGTT TGGTCAGTGC TATTTCTACC CATCTGCACC 
ACAGTATTTA TAATTTTCAG CAGGATTGAT TTGTTTCATC CTATACAGTG GCTGTCTGAT 
TCTTTCAGTG ACCTGTATAG TTCCTATGTA ATCTTTTACT TCCTGCTGCT GTCAGTGGTA 
ATAATAATAA TAAGTATTTT CAATGTGGAG TTCTATGCAG TGATAACCCA GGGCCAGTAC 
AGCTTTCTTG TGGTTCCCTG CACTGGTACT AACAGCTTTG GTAGCCCTGC TGCGCAAACC 
TGCTTAAATG AATATCATCT TTTTTTCCTA CTGACTGGAG CATTTATGGG CTATAGCTAT 
AGCCTCCTGT ATTTTGTTAA CAACATGAAC TATCTTCCAT TTCCCATCAT ACAGCAATAC 
AAGTTCTTGC GTTTTAGGAG ATCTCTGCTC TTATTAGTTA AACACAGTTG TGTGGAATCA 
CTGTTCCTGG TTAGAAATTT CTGCATTTTA TATTATTTTC TTGGCTATAT TCCCAAAGCT 
TGGATTAGCA CTGCTATGAA CCTTCACATA GATGAGCAGG TTCATAGGCC ACTTGACACA 
GTGAGTGGCC TCTTAAATCT CTCGTTACTC TACCATGTCT GGCTGTGTGG TGTCTTTCTC 
CTGACGACTT GGTATGTCTC ATGGATACTC TTCAAAATCT ATGCCACAGA GGCTCATGTG 
TTTCCTGTTC AACCACCATT TGCAGAAGGG TCAGATGAGT GCCTTCCAAA AGTGTTAAAT 
AGCAATCCTC CCCCCATCAT AAAGTATTTA GCCTTGCAGG ACCTGATGTT GCTTTCTCAA 
TATTCTCCTT CACGAAGACA AGAAGTTTTC AGCCTCAGCC AACCAGGTGG ACATCCCCAC 
AATTGGACAG CCATTTCAAG GGAGTGTTTG AATCTTTTAA ATGGTATGAC TCAGAAACTG 
ATTCTCTATC AAGAAGCTGC TGCTACGAAT GGGAGAGTGT CTTCATCTTA CCCAGTGGAA 
CCTAAGAAAT TAAATTCTCC AGAAGAAACT GCTTTTCAGA CACCAAAATC TAGCCAGATG 
CCTCGGCCTT CAGTGCCACC ATTAGTTAAA ACATCACTGT TTTCTTCAAA ATTATCTACA 
CCTGATGTTG TGAGCCCATT TGGGACCCCA TTTGGCTCTA GTGTAATGAA TCGGATGGCT 
GGAATTTTTG ATGTAAACAC CTGCTATGGG TCACCGCAAA GTCCTCAGCT AATAAGAAGG 
GGGCCAAGAT TGTGGACATC AGCTTCTGAT CAGCAAATGA CTGAATTTTC TAATCCTTCT 
CCATCTACCT CTATTAGTGC TGAGGGTAAG ACAATGAGAC AACCCAGTGT GATTTATTCA 
TGGATTCAGA ATAAACGTGA ACAGATTAAG AATTTCTTGT CAAAACGGGT GCTGATAATG 
TATTTTTTCA GTAAGCACCC AGAGGCCTCC ATTCAGGCTG TTTTTTCAGA TGCCCAAATG 
CATATTTGGG CATTAGAAGG TCTGTCGCAC TTAGTAGCAG CATCATTTAC AGAGGATAGA 
TTTGGAGTTG TCCAGACGAC ACTACCAGCT ATCCTTAATA CTTTGTTGAC ACTGCAAGAG 
GCAGTCGACA AGTACTTTAA GCTTCCTCAT GCTTCCAGTA AACCACCCCG GATTTCAGGA 
AGCCTTGTGG ACACTTCATA TAAAACATTA AGATTTGCAT TCAGAGCATC ACTGAAAACT 
GCCATCTATC GAATAACTAC TACATTTGGT GAACATCTGA ATGCTGTGCA AGCATCTGCA 
GAACATCAGA AAAGACTTCA ACAGTTCTTG GAGTTCAAAG AATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu32206
Clone ID Related Accession (Same CDS sequence) NM_001168551.1 , NM_001168551.2
Accession Version NM_001168551.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1905bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-05-30
Organism Homo sapiens(human)
Product nucleoporin NDC1 isoform 2
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK295000.1, BC003082.2 and AL354612.1. On May 31, 2019 this sequence version replaced NM_001168551.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.173212.1, SRR1803615.269164.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGGCCACGG CCGTGAGCCG GCCCTGCGCC GGCAGGTCGC GGGACATACT GTGGCGCGTT 
TTGGGCTGGA GGATAGTTGC AAGTATTGTT TGGTCAGTGC TATTTCTACC CATCTGCACC 
ACAGTATTTA TAATTTTCAG CAGGATTGAT TTGTTTCATC CTATACAGTG GCTGTCTGAT 
TCTTTCAGTG ACCTGTATAG TTCCTATGTA ATCTTTTACT TCCTGCTGCT GTCAGTGGTA 
ATAATAATAA TAAGTATTTT CAATGTGGAG TTCTATGCAG TGATAACCCA GGGCCAGTAC 
AGCTTTCTTG TGGTTCCCTG CACTGGTACT AACAGCTTTG GTAGCCCTGC TGCGCAAACC 
TGCTTAAATG AATATCATCT TTTTTTCCTA CTGACTGGAG CATTTATGGG CTATAGCTAT 
AGCCTCCTGT ATTTTGTTAA CAACATGAAC TATCTTCCAT TTCCCATCAT ACAGCAATAC 
AAGTTCTTGC GTTTTAGGAG ATCTCTGCTC TTATTAGTTA AACACAGTTG TGTGGAATCA 
CTGTTCCTGG TTAGAAATTT CTGCATTTTA TATTATTTTC TTGGCTATAT TCCCAAAGCT 
TGGATTAGCA CTGCTATGAA CCTTCACATA GATGAGCAGG TTCATAGGCC ACTTGACACA 
GTGAGTGGCC TCTTAAATCT CTCGTTACTC TACCATGTCT GGCTGTGTGG TGTCTTTCTC 
CTGACGACTT GGTATGTCTC ATGGATACTC TTCAAAATCT ATGCCACAGA GGCTCATGTG 
TTTCCTGTTC AACCACCATT TGCAGAAGGG TCAGATGAGT GCCTTCCAAA AGTGTTAAAT 
AGCAATCCTC CCCCCATCAT AAAGTATTTA GCCTTGCAGG ACCTGATGTT GCTTTCTCAA 
TATTCTCCTT CACGAAGACA AGAAGTTTTC AGCCTCAGCC AACCAGGTGG ACATCCCCAC 
AATTGGACAG CCATTTCAAG GGAGTGTTTG AATCTTTTAA ATGGTATGAC TCAGAAACTG 
ATTCTCTATC AAGAAGCTGC TGCTACGAAT GGGAGAGTGT CTTCATCTTA CCCAGTGGAA 
CCTAAGAAAT TAAATTCTCC AGAAGAAACT GCTTTTCAGA CACCAAAATC TAGCCAGATG 
CCTCGGCCTT CAGTGCCACC ATTAGTTAAA ACATCACTGT TTTCTTCAAA ATTATCTACA 
CCTGATGTTG TGAGCCCATT TGGGACCCCA TTTGGCTCTA GTGTAATGAA TCGGATGGCT 
GGAATTTTTG ATGTAAACAC CTGCTATGGG TCACCGCAAA GTCCTCAGCT AATAAGAAGG 
GGGCCAAGAT TGTGGACATC AGCTTCTGAT CAGCAAATGA CTGAATTTTC TAATCCTTCT 
CCATCTACCT CTATTAGTGC TGAGGGTAAG ACAATGAGAC AACCCAGTGT GATTTATTCA 
TGGATTCAGA ATAAACGTGA ACAGATTAAG AATTTCTTGT CAAAACGGGT GCTGATAATG 
TATTTTTTCA GTAAGCACCC AGAGGCCTCC ATTCAGGCTG TTTTTTCAGA TGCCCAAATG 
CATATTTGGG CATTAGAAGG TCTGTCGCAC TTAGTAGCAG CATCATTTAC AGAGGATAGA 
TTTGGAGTTG TCCAGACGAC ACTACCAGCT ATCCTTAATA CTTTGTTGAC ACTGCAAGAG 
GCAGTCGACA AGTACTTTAA GCTTCCTCAT GCTTCCAGTA AACCACCCCG GATTTCAGGA 
AGCCTTGTGG ACACTTCATA TAAAACATTA AGATTTGCAT TCAGAGCATC ACTGAAAACT 
GCCATCTATC GAATAACTAC TACATTTGGT GAACATCTGA ATGCTGTGCA AGCATCTGCA 
GAACATCAGA AAAGACTTCA ACAGTTCTTG GAGTTCAAAG AATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001162023.1
CDS36..1940
Translation

Target ORF information:

RefSeq Version NM_001168551.2
Organism Homo sapiens(human)
Definition Homo sapiens NDC1 transmembrane nucleoporin (NDC1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001168551.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGGCCACGG CCGTGAGCCG GCCCTGCGCC GGCAGGTCGC GGGACATACT GTGGCGCGTT 
TTGGGCTGGA GGATAGTTGC AAGTATTGTT TGGTCAGTGC TATTTCTACC CATCTGCACC 
ACAGTATTTA TAATTTTCAG CAGGATTGAT TTGTTTCATC CTATACAGTG GCTGTCTGAT 
TCTTTCAGTG ACCTGTATAG TTCCTATGTA ATCTTTTACT TCCTGCTGCT GTCAGTGGTA 
ATAATAATAA TAAGTATTTT CAATGTGGAG TTCTATGCAG TGATAACCCA GGGCCAGTAC 
AGCTTTCTTG TGGTTCCCTG CACTGGTACT AACAGCTTTG GTAGCCCTGC TGCGCAAACC 
TGCTTAAATG AATATCATCT TTTTTTCCTA CTGACTGGAG CATTTATGGG CTATAGCTAT 
AGCCTCCTGT ATTTTGTTAA CAACATGAAC TATCTTCCAT TTCCCATCAT ACAGCAATAC 
AAGTTCTTGC GTTTTAGGAG ATCTCTGCTC TTATTAGTTA AACACAGTTG TGTGGAATCA 
CTGTTCCTGG TTAGAAATTT CTGCATTTTA TATTATTTTC TTGGCTATAT TCCCAAAGCT 
TGGATTAGCA CTGCTATGAA CCTTCACATA GATGAGCAGG TTCATAGGCC ACTTGACACA 
GTGAGTGGCC TCTTAAATCT CTCGTTACTC TACCATGTCT GGCTGTGTGG TGTCTTTCTC 
CTGACGACTT GGTATGTCTC ATGGATACTC TTCAAAATCT ATGCCACAGA GGCTCATGTG 
TTTCCTGTTC AACCACCATT TGCAGAAGGG TCAGATGAGT GCCTTCCAAA AGTGTTAAAT 
AGCAATCCTC CCCCCATCAT AAAGTATTTA GCCTTGCAGG ACCTGATGTT GCTTTCTCAA 
TATTCTCCTT CACGAAGACA AGAAGTTTTC AGCCTCAGCC AACCAGGTGG ACATCCCCAC 
AATTGGACAG CCATTTCAAG GGAGTGTTTG AATCTTTTAA ATGGTATGAC TCAGAAACTG 
ATTCTCTATC AAGAAGCTGC TGCTACGAAT GGGAGAGTGT CTTCATCTTA CCCAGTGGAA 
CCTAAGAAAT TAAATTCTCC AGAAGAAACT GCTTTTCAGA CACCAAAATC TAGCCAGATG 
CCTCGGCCTT CAGTGCCACC ATTAGTTAAA ACATCACTGT TTTCTTCAAA ATTATCTACA 
CCTGATGTTG TGAGCCCATT TGGGACCCCA TTTGGCTCTA GTGTAATGAA TCGGATGGCT 
GGAATTTTTG ATGTAAACAC CTGCTATGGG TCACCGCAAA GTCCTCAGCT AATAAGAAGG 
GGGCCAAGAT TGTGGACATC AGCTTCTGAT CAGCAAATGA CTGAATTTTC TAATCCTTCT 
CCATCTACCT CTATTAGTGC TGAGGGTAAG ACAATGAGAC AACCCAGTGT GATTTATTCA 
TGGATTCAGA ATAAACGTGA ACAGATTAAG AATTTCTTGT CAAAACGGGT GCTGATAATG 
TATTTTTTCA GTAAGCACCC AGAGGCCTCC ATTCAGGCTG TTTTTTCAGA TGCCCAAATG 
CATATTTGGG CATTAGAAGG TCTGTCGCAC TTAGTAGCAG CATCATTTAC AGAGGATAGA 
TTTGGAGTTG TCCAGACGAC ACTACCAGCT ATCCTTAATA CTTTGTTGAC ACTGCAAGAG 
GCAGTCGACA AGTACTTTAA GCTTCCTCAT GCTTCCAGTA AACCACCCCG GATTTCAGGA 
AGCCTTGTGG ACACTTCATA TAAAACATTA AGATTTGCAT TCAGAGCATC ACTGAAAACT 
GCCATCTATC GAATAACTAC TACATTTGGT GAACATCTGA ATGCTGTGCA AGCATCTGCA 
GAACATCAGA AAAGACTTCA ACAGTTCTTG GAGTTCAAAG AATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu69653
Clone ID Related Accession (Same CDS sequence) XM_011541766.2
Accession Version XM_011541766.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2022bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product nucleoporin NDC1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011541766.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGAAATTG GAATAGGGAG TGAGGCGGAG TTTTACGCAA GGGTACCGTA TCCAGTTTTG 
GGCTGGAGGA TAGTTGCAAG TATTGTTTGG TCAGTGCTAT TTCTACCCAT CTGCACCACA 
GTATTTATAA TTTTCAGCAG GATTGATTTG TTTCATCCTA TACAGTGGCT GTCTGATTCT 
TTCAGTGACC TGTATAGTTC CTATGTAATC TTTTACTTCC TGCTGCTGTC AGTGGTAATA 
ATAATAATAA GTATTTTCAA TGTGGAGTTC TATGCAGTTG TGCCTTCTAT TCCTTGCTCC 
AGACTAGCTC TGATAGGGAA GATCATTCAT CCTCAGCAAC TCATGCACTC ATTTATTCAT 
GCTGCAATGG GAATGGTGAT GGCCTGGTGT GCTGCAGTGA TAACCCAGGG CCAGTACAGC 
TTTCTTGTGG TTCCCTGCAC TGGTACTAAC AGCTTTGGTA GCCCTGCTGC GCAAACCTGC 
TTAAATGAAT ATCATCTTTT TTTCCTACTG ACTGGAGCAT TTATGGGCTA TAGCTATAGC 
CTCCTGTATT TTGTTAACAA CATGAACTAT CTTCCATTTC CCATCATACA GCAATACAAG 
TTCTTGCGTT TTAGGAGATC TCTGCTCTTA TTAGTTAAAC ACAGTTGTGT GGAATCACTG 
TTCCTGGTTA GAAATTTCTG CATTTTATAT TATTTTCTTG GCTATATTCC CAAAGCTTGG 
ATTAGCACTG CTATGAACCT TCACATAGAT GAGCAGGTTC ATAGGCCACT TGACACAGTG 
AGTGGCCTCT TAAATCTCTC GTTACTCTAC CATGTCTGGC TGTGTGGTGT CTTTCTCCTG 
ACGACTTGGT ATGTCTCATG GATACTCTTC AAAATCTATG CCACAGAGGC TCATGTGTTT 
CCTGTTCAAC CACCATTTGC AGAAGGGTCA GATGAGTGCC TTCCAAAAGT GTTAAATAGC 
AATCCTCCCC CCATCATAAA GTATTTAGCC TTGCAGGACC TGATGTTGCT TTCTCAATAT 
TCTCCTTCAC GAAGACAAGA AGTTTTCAGC CTCAGCCAAC CAGGTGGACA TCCCCACAAT 
TGGACAGCCA TTTCAAGGGA GTGTTTGAAT CTTTTAAATG GTATGACTCA GAAACTGATT 
CTCTATCAAG AAGCTGCTGC TACGAATGGG AGAGTGTCTT CATCTTACCC AGTGGAACCT 
AAGAAATTAA ATTCTCCAGA AGAAACTGCT TTTCAGACAC CAAAATCTAG CCAGATGCCT 
CGGCCTTCAG TGCCACCATT AGTTAAAACA TCACTGTTTT CTTCAAAATT ATCTACACCT 
GATGTTGTGA GCCCATTTGG GACCCCATTT GGCTCTAGTG TAATGAATCG GATGGCTGGA 
ATTTTTGATG TAAACACCTG CTATGGGTCA CCGCAAAGTC CTCAGCTAAT AAGAAGGGGG 
CCAAGATTGT GGACATCAGC TTCTGATCAG CAAATGACTG AATTTTCTAA TCCTTCTCCA 
TCTACCTCTA TTAGTGCTGA GGGTAAGACA ATGAGACAAC CCAGTGTGAT TTATTCATGG 
ATTCAGAATA AACGTGAACA GATTAAGAAT TTCTTGTCAA AACGGGTGCT GATAATGTAT 
TTTTTCAGTA AGCACCCAGA GGCCTCCATT CAGGCTGTTT TTTCAGATGC CCAAATGCAT 
ATTTGGGCAT TAGAAGGTCT GTCGCACTTA GTAGCAGCAT CATTTACAGA GGATAGATTT 
GGAGTTGTCC AGACGACACT ACCAGCTATC CTTAATACTT TGTTGACACT GCAAGAGGCA 
GTCGACAAGT ACTTTAAGCT TCCTCATGCT TCCAGTAAAC CACCCCGGAT TTCAGGAAGC 
CTTGTGGACA CTTCATATAA AACATTAAGA TTTGCATTCA GAGCATCACT GAAAACTGCC 
ATCTATCGAA TAACTACTAC ATTTGGTGAA CATCTGAATG CTGTGCAAGC ATCTGCAGAA 
CATCAGAAAA GACTTCAACA GTTCTTGGAG TTCAAAGAAT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011540068.1
CDS137..2158
Translation

Target ORF information:

RefSeq Version XM_011541766.2
Organism Homo sapiens(human)
Definition Homo sapiens NDC1 transmembrane nucleoporin (NDC1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011541766.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGAAATTG GAATAGGGAG TGAGGCGGAG TTTTACGCAA GGGTACCGTA TCCAGTTTTG 
GGCTGGAGGA TAGTTGCAAG TATTGTTTGG TCAGTGCTAT TTCTACCCAT CTGCACCACA 
GTATTTATAA TTTTCAGCAG GATTGATTTG TTTCATCCTA TACAGTGGCT GTCTGATTCT 
TTCAGTGACC TGTATAGTTC CTATGTAATC TTTTACTTCC TGCTGCTGTC AGTGGTAATA 
ATAATAATAA GTATTTTCAA TGTGGAGTTC TATGCAGTTG TGCCTTCTAT TCCTTGCTCC 
AGACTAGCTC TGATAGGGAA GATCATTCAT CCTCAGCAAC TCATGCACTC ATTTATTCAT 
GCTGCAATGG GAATGGTGAT GGCCTGGTGT GCTGCAGTGA TAACCCAGGG CCAGTACAGC 
TTTCTTGTGG TTCCCTGCAC TGGTACTAAC AGCTTTGGTA GCCCTGCTGC GCAAACCTGC 
TTAAATGAAT ATCATCTTTT TTTCCTACTG ACTGGAGCAT TTATGGGCTA TAGCTATAGC 
CTCCTGTATT TTGTTAACAA CATGAACTAT CTTCCATTTC CCATCATACA GCAATACAAG 
TTCTTGCGTT TTAGGAGATC TCTGCTCTTA TTAGTTAAAC ACAGTTGTGT GGAATCACTG 
TTCCTGGTTA GAAATTTCTG CATTTTATAT TATTTTCTTG GCTATATTCC CAAAGCTTGG 
ATTAGCACTG CTATGAACCT TCACATAGAT GAGCAGGTTC ATAGGCCACT TGACACAGTG 
AGTGGCCTCT TAAATCTCTC GTTACTCTAC CATGTCTGGC TGTGTGGTGT CTTTCTCCTG 
ACGACTTGGT ATGTCTCATG GATACTCTTC AAAATCTATG CCACAGAGGC TCATGTGTTT 
CCTGTTCAAC CACCATTTGC AGAAGGGTCA GATGAGTGCC TTCCAAAAGT GTTAAATAGC 
AATCCTCCCC CCATCATAAA GTATTTAGCC TTGCAGGACC TGATGTTGCT TTCTCAATAT 
TCTCCTTCAC GAAGACAAGA AGTTTTCAGC CTCAGCCAAC CAGGTGGACA TCCCCACAAT 
TGGACAGCCA TTTCAAGGGA GTGTTTGAAT CTTTTAAATG GTATGACTCA GAAACTGATT 
CTCTATCAAG AAGCTGCTGC TACGAATGGG AGAGTGTCTT CATCTTACCC AGTGGAACCT 
AAGAAATTAA ATTCTCCAGA AGAAACTGCT TTTCAGACAC CAAAATCTAG CCAGATGCCT 
CGGCCTTCAG TGCCACCATT AGTTAAAACA TCACTGTTTT CTTCAAAATT ATCTACACCT 
GATGTTGTGA GCCCATTTGG GACCCCATTT GGCTCTAGTG TAATGAATCG GATGGCTGGA 
ATTTTTGATG TAAACACCTG CTATGGGTCA CCGCAAAGTC CTCAGCTAAT AAGAAGGGGG 
CCAAGATTGT GGACATCAGC TTCTGATCAG CAAATGACTG AATTTTCTAA TCCTTCTCCA 
TCTACCTCTA TTAGTGCTGA GGGTAAGACA ATGAGACAAC CCAGTGTGAT TTATTCATGG 
ATTCAGAATA AACGTGAACA GATTAAGAAT TTCTTGTCAA AACGGGTGCT GATAATGTAT 
TTTTTCAGTA AGCACCCAGA GGCCTCCATT CAGGCTGTTT TTTCAGATGC CCAAATGCAT 
ATTTGGGCAT TAGAAGGTCT GTCGCACTTA GTAGCAGCAT CATTTACAGA GGATAGATTT 
GGAGTTGTCC AGACGACACT ACCAGCTATC CTTAATACTT TGTTGACACT GCAAGAGGCA 
GTCGACAAGT ACTTTAAGCT TCCTCATGCT TCCAGTAAAC CACCCCGGAT TTCAGGAAGC 
CTTGTGGACA CTTCATATAA AACATTAAGA TTTGCATTCA GAGCATCACT GAAAACTGCC 
ATCTATCGAA TAACTACTAC ATTTGGTGAA CATCTGAATG CTGTGCAAGC ATCTGCAGAA 
CATCAGAAAA GACTTCAACA GTTCTTGGAG TTCAAAGAAT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

SEPT12-NDC1 Complexes Are Required for Mammalian Spermiogenesis.
International journal of molecular sciences17(11)(2016 Nov)
Lai TH,Wu YY,Wang YY,Chen MF,Wang P,Chen TM,Wu YN,Chiang HS,Kuo PL,Lin YH


book

Overexpression and biological function of TMEM48 in non-small cell lung carcinoma.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine37(2)2575-86(2016 Feb)
Qiao W,Han Y,Jin W,Tian M,Chen P,Min J,Hu H,Xu B,Zhu W,Xiong L,Lin Q


book

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA


book

The nuclear pore complex protein ALADIN is anchored via NDC1 but not via POM121 and GP210 in the nuclear envelope.
Biochemical and biophysical research communications390(2)205-10(2009 Dec)
Kind B,Koehler K,Lorenz M,Huebner A