This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined. [provided by RefSeq, Jul 2008].
The following PCDHA4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PCDHA4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005609.1, AB208925.1, BC112102.1 and KF458021.1. On May 31, 2019 this sequence version replaced NM_031500.2.
Transcript Variant: This variant (2) utilizes the large, first exon then continues into the downstream intron 1 sequence before terminating. This one-exon transcript encodes a shorter isoform (2), compared to isoform 1. This short variant is represented based on data in PMID:10380929.
##Evidence-Data-START##
Transcript is intronless :: BC113609.1, AF152482.1 [ECO:0000345]
##Evidence-Data-END##
Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB208925.1 and BC112102.1. On Jul 22, 2013 this sequence version replaced NM_031500.1.
Transcript Variant: This variant (2) utilizes the large, first exon then continues into the downstream intron 1 sequence before terminating. This one-exon transcript encodes a shorter isoform (2), compared to isoform 1. This short variant is represented based on data in PMID:10380929.
Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005609.1, AB208925.1, AF152312.1 and AC010223.6. On Nov 22, 2018 this sequence version replaced NM_018907.3.
Transcript Variant: This variant (1) includes the constant region exons and encodes the longest isoform (1).
##Evidence-Data-START##
Transcript exon combination :: SRR1660807.8381.1, SRR1803613.230692.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2146982 [ECO:0000348]
##Evidence-Data-END##
##RefSeq-Attributes-START##
MANE Ensembl match :: ENST00000530339.2/ ENSP00000435300.1
RefSeq Select criteria :: based on conservation, expression, longest protein
##RefSeq-Attributes-END##
COMPLETENESS: full length.
Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB208925.1 and AF152312.1. On Jul 22, 2013 this sequence version replaced NM_018907.2.
Transcript Variant: This variant (1) includes the constant region exons and encodes the longest isoform (1).
##Evidence-Data-START##
Transcript exon combination :: SRR1803611.167581.1, SRR1803613.230692.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns SAMEA2144335, SAMEA2145743 [ECO:0000348]
##Evidence-Data-END##
COMPLETENESS: complete on the 3' end.
User Manual
NM_031500.3
