NCLN cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following NCLN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCLN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu29750 NM_020170.4
Latest version!
Homo sapiens nicalin (NCLN), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu51703 NM_001321463.2
Latest version!
Homo sapiens nicalin (NCLN), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
View Old Accession Versions >>
OHu51703 NM_001321463.1 Homo sapiens nicalin (NCLN), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu29750 NM_020170.3 Homo sapiens nicalin (NCLN), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu29750
    Clone ID Related Accession (Same CDS sequence) NM_020170.4 , NM_020170.3
    Accession Version NM_020170.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1692bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-01
    Organism Homo sapiens(human)
    Product nicalin isoform 1 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC013283.2, AL136567.1, BC008920.2, BC010064.2, BC003076.2, AL365369.1, BC025926.1 and BC007275.1. On Nov 23, 2018 this sequence version replaced NM_020170.3. ##Evidence-Data-START## Transcript exon combination :: AK172848.1, AL136567.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000246117.9/ ENSP00000246117.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCTGGAGG AAGCGGGCGA GGTGCTGGAG AACATGCTGA AGGCGTCTTG TCTGCCGCTC 
    GGCTTCATCG TCTTCCTGCC CGCTGTGCTG CTGCTGGTGG CGCCGCCGCT GCCTGCCGCC
    GACGCCGCGC ACGAGTTCAC CGTGTACCGC ATGCAGCAGT ACGACCTGCA GGGCCAGCCC
    TACGGCACAC GGAATGCAGT GCTGAACACG GAGGCGCGCA CGATGGCGGC GGAGGTGCTG
    AGCCGCCGCT GCGTGCTCAT GCGGCTACTG GACTTCTCCT ACGAGCAGTA CCAGAAGGCC
    CTGCGGCAGT CGGCGGGCGC CGTGGTCATC ATCCTGCCCA GGGCCATGGC CGCCGTGCCC
    CAGGACGTCG TCCGGCAATT CATGGAGATC GAGCCGGAGA TGCTGGCCAT GGAGACCGCC
    GTCCCCGTGT ACTTTGCCGT GGAGGACGAG GCCCTGCTGT CTATCTACAA GCAGACCCAG
    GCTGCCTCCG CCTCCCAGGG CTCCGCCTCT GCTGCTGAAG TACTGCTGCG CACGGCCACT
    GCCAACGGCT TCCAGATGGT CACCAGCGGG GTACAGAGCA AGGCCGTGAG TGACTGGCTG
    ATTGCCAGCG TGGAGGGGCG GCTGACGGGG CTGGGCGGAG AGGACCTTCC CACCATCGTC
    ATCGTGGCCC ACTACGACGC CTTTGGAGTG GCCCCCTGGC TGTCGCTGGG CGCGGACTCC
    AACGGGAGCG GCGTCTCTGT GCTGCTGGAG CTGGCACGCC TCTTCTCCCG GCTCTACACC
    TACAAGCGCA CGCACGCCGC CTACAACCTC CTGTTCTTTG CGTCTGGAGG AGGCAAGTTT
    AACTACCAGG GAACCAAGCG CTGGCTGGAA GACAACCTGG ACCACACAGA CTCCAGCCTG
    CTTCAGGACA ATGTGGCCTT CGTGCTGTGC CTGGACACCG TGGGCCGGGG CAGCAGCCTG
    CACCTGCACG TGTCCAAGCC GCCTCGGGAG GGCACCCTGC AGCACGCCTT CCTGCGGGAG
    CTGGAGACGG TGGCCGCGCA CCAGTTCCCT GAGGTACGGT TCTCCATGGT GCACAAGCGG
    ATCAACCTGG CGGAGGACGT GCTGGCCTGG GAGCACGAGC GCTTCGCCAT CCGCCGACTG
    CCCGCCTTCA CGCTGTCCCA CCTGGAGAGC CACCGTGACG GCCAGCGCAG CAGCATCATG
    GACGTGCGGT CCCGGGTGGA TTCTAAGACC CTGACCCGTA ACACGAGGAT CATTGCAGAG
    GCCCTGACTC GAGTCATCTA CAACCTGACA GAGAAGGGGA CACCCCCAGA CATGCCGGTG
    TTCACAGAGC AGATGCAGAT CCAGCAGGAG CAGCTGGACT CGGTGATGGA CTGGCTCACC
    AACCAGCCGC GGGCCGCGCA GCTGGTGGAC AAGGACAGCA CCTTCCTCAG CACGCTGGAG
    CACCACCTGA GCCGCTACCT GAAGGACGTG AAGCAGCACC ACGTCAAGGC TGACAAGCGG
    GACCCAGAGT TTGTCTTCTA CGACCAGCTG AAGCAAGTGA TGAATGCGTA CAGAGTCAAG
    CCGGCCGTCT TTGACCTGCT CCTGGCTGTT GGCATTGCTG CCTACCTCGG CATGGCCTAC
    GTGGCTGTCC AGCACTTCAG CCTCCTCTAC AAGACCGTCC AGAGGCTGCT CGTGAAGGCC
    AAGACACAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_064555.2
    CDS102..1793
    Translation

    Target ORF information:

    RefSeq Version NM_020170.4
    Organism Homo sapiens(human)
    Definition Homo sapiens nicalin (NCLN), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_020170.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCTGGAGG AAGCGGGCGA GGTGCTGGAG AACATGCTGA AGGCGTCTTG TCTGCCGCTC 
    GGCTTCATCG TCTTCCTGCC CGCTGTGCTG CTGCTGGTGG CGCCGCCGCT GCCTGCCGCC
    GACGCCGCGC ACGAGTTCAC CGTGTACCGC ATGCAGCAGT ACGACCTGCA GGGCCAGCCC
    TACGGCACAC GGAATGCAGT GCTGAACACG GAGGCGCGCA CGATGGCGGC GGAGGTGCTG
    AGCCGCCGCT GCGTGCTCAT GCGGCTACTG GACTTCTCCT ACGAGCAGTA CCAGAAGGCC
    CTGCGGCAGT CGGCGGGCGC CGTGGTCATC ATCCTGCCCA GGGCCATGGC CGCCGTGCCC
    CAGGACGTCG TCCGGCAATT CATGGAGATC GAGCCGGAGA TGCTGGCCAT GGAGACCGCC
    GTCCCCGTGT ACTTTGCCGT GGAGGACGAG GCCCTGCTGT CTATCTACAA GCAGACCCAG
    GCTGCCTCCG CCTCCCAGGG CTCCGCCTCT GCTGCTGAAG TACTGCTGCG CACGGCCACT
    GCCAACGGCT TCCAGATGGT CACCAGCGGG GTACAGAGCA AGGCCGTGAG TGACTGGCTG
    ATTGCCAGCG TGGAGGGGCG GCTGACGGGG CTGGGCGGAG AGGACCTTCC CACCATCGTC
    ATCGTGGCCC ACTACGACGC CTTTGGAGTG GCCCCCTGGC TGTCGCTGGG CGCGGACTCC
    AACGGGAGCG GCGTCTCTGT GCTGCTGGAG CTGGCACGCC TCTTCTCCCG GCTCTACACC
    TACAAGCGCA CGCACGCCGC CTACAACCTC CTGTTCTTTG CGTCTGGAGG AGGCAAGTTT
    AACTACCAGG GAACCAAGCG CTGGCTGGAA GACAACCTGG ACCACACAGA CTCCAGCCTG
    CTTCAGGACA ATGTGGCCTT CGTGCTGTGC CTGGACACCG TGGGCCGGGG CAGCAGCCTG
    CACCTGCACG TGTCCAAGCC GCCTCGGGAG GGCACCCTGC AGCACGCCTT CCTGCGGGAG
    CTGGAGACGG TGGCCGCGCA CCAGTTCCCT GAGGTACGGT TCTCCATGGT GCACAAGCGG
    ATCAACCTGG CGGAGGACGT GCTGGCCTGG GAGCACGAGC GCTTCGCCAT CCGCCGACTG
    CCCGCCTTCA CGCTGTCCCA CCTGGAGAGC CACCGTGACG GCCAGCGCAG CAGCATCATG
    GACGTGCGGT CCCGGGTGGA TTCTAAGACC CTGACCCGTA ACACGAGGAT CATTGCAGAG
    GCCCTGACTC GAGTCATCTA CAACCTGACA GAGAAGGGGA CACCCCCAGA CATGCCGGTG
    TTCACAGAGC AGATGCAGAT CCAGCAGGAG CAGCTGGACT CGGTGATGGA CTGGCTCACC
    AACCAGCCGC GGGCCGCGCA GCTGGTGGAC AAGGACAGCA CCTTCCTCAG CACGCTGGAG
    CACCACCTGA GCCGCTACCT GAAGGACGTG AAGCAGCACC ACGTCAAGGC TGACAAGCGG
    GACCCAGAGT TTGTCTTCTA CGACCAGCTG AAGCAAGTGA TGAATGCGTA CAGAGTCAAG
    CCGGCCGTCT TTGACCTGCT CCTGGCTGTT GGCATTGCTG CCTACCTCGG CATGGCCTAC
    GTGGCTGTCC AGCACTTCAG CCTCCTCTAC AAGACCGTCC AGAGGCTGCT CGTGAAGGCC
    AAGACACAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu29750
    Clone ID Related Accession (Same CDS sequence) NM_020170.4 , NM_020170.3
    Accession Version NM_020170.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1692bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-30
    Organism Homo sapiens(human)
    Product nicalin isoform 1 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC013283.2, AL136567.1, BC008920.2, BC010064.2, BC003076.2, AL365369.1, BC025926.1 and BC007275.1. On Sep 21, 2004 this sequence version replaced NM_020170.2. ##Evidence-Data-START## Transcript exon combination :: AL136567.1, BC013283.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCTGGAGG AAGCGGGCGA GGTGCTGGAG AACATGCTGA AGGCGTCTTG TCTGCCGCTC 
    GGCTTCATCG TCTTCCTGCC CGCTGTGCTG CTGCTGGTGG CGCCGCCGCT GCCTGCCGCC
    GACGCCGCGC ACGAGTTCAC CGTGTACCGC ATGCAGCAGT ACGACCTGCA GGGCCAGCCC
    TACGGCACAC GGAATGCAGT GCTGAACACG GAGGCGCGCA CGATGGCGGC GGAGGTGCTG
    AGCCGCCGCT GCGTGCTCAT GCGGCTACTG GACTTCTCCT ACGAGCAGTA CCAGAAGGCC
    CTGCGGCAGT CGGCGGGCGC CGTGGTCATC ATCCTGCCCA GGGCCATGGC CGCCGTGCCC
    CAGGACGTCG TCCGGCAATT CATGGAGATC GAGCCGGAGA TGCTGGCCAT GGAGACCGCC
    GTCCCCGTGT ACTTTGCCGT GGAGGACGAG GCCCTGCTGT CTATCTACAA GCAGACCCAG
    GCTGCCTCCG CCTCCCAGGG CTCCGCCTCT GCTGCTGAAG TACTGCTGCG CACGGCCACT
    GCCAACGGCT TCCAGATGGT CACCAGCGGG GTACAGAGCA AGGCCGTGAG TGACTGGCTG
    ATTGCCAGCG TGGAGGGGCG GCTGACGGGG CTGGGCGGAG AGGACCTTCC CACCATCGTC
    ATCGTGGCCC ACTACGACGC CTTTGGAGTG GCCCCCTGGC TGTCGCTGGG CGCGGACTCC
    AACGGGAGCG GCGTCTCTGT GCTGCTGGAG CTGGCACGCC TCTTCTCCCG GCTCTACACC
    TACAAGCGCA CGCACGCCGC CTACAACCTC CTGTTCTTTG CGTCTGGAGG AGGCAAGTTT
    AACTACCAGG GAACCAAGCG CTGGCTGGAA GACAACCTGG ACCACACAGA CTCCAGCCTG
    CTTCAGGACA ATGTGGCCTT CGTGCTGTGC CTGGACACCG TGGGCCGGGG CAGCAGCCTG
    CACCTGCACG TGTCCAAGCC GCCTCGGGAG GGCACCCTGC AGCACGCCTT CCTGCGGGAG
    CTGGAGACGG TGGCCGCGCA CCAGTTCCCT GAGGTACGGT TCTCCATGGT GCACAAGCGG
    ATCAACCTGG CGGAGGACGT GCTGGCCTGG GAGCACGAGC GCTTCGCCAT CCGCCGACTG
    CCCGCCTTCA CGCTGTCCCA CCTGGAGAGC CACCGTGACG GCCAGCGCAG CAGCATCATG
    GACGTGCGGT CCCGGGTGGA TTCTAAGACC CTGACCCGTA ACACGAGGAT CATTGCAGAG
    GCCCTGACTC GAGTCATCTA CAACCTGACA GAGAAGGGGA CACCCCCAGA CATGCCGGTG
    TTCACAGAGC AGATGCAGAT CCAGCAGGAG CAGCTGGACT CGGTGATGGA CTGGCTCACC
    AACCAGCCGC GGGCCGCGCA GCTGGTGGAC AAGGACAGCA CCTTCCTCAG CACGCTGGAG
    CACCACCTGA GCCGCTACCT GAAGGACGTG AAGCAGCACC ACGTCAAGGC TGACAAGCGG
    GACCCAGAGT TTGTCTTCTA CGACCAGCTG AAGCAAGTGA TGAATGCGTA CAGAGTCAAG
    CCGGCCGTCT TTGACCTGCT CCTGGCTGTT GGCATTGCTG CCTACCTCGG CATGGCCTAC
    GTGGCTGTCC AGCACTTCAG CCTCCTCTAC AAGACCGTCC AGAGGCTGCT CGTGAAGGCC
    AAGACACAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_064555.2
    CDS155..1846
    Translation

    Target ORF information:

    RefSeq Version NM_020170.3
    Organism Homo sapiens(human)
    Definition Homo sapiens nicalin (NCLN), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_020170.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCTGGAGG AAGCGGGCGA GGTGCTGGAG AACATGCTGA AGGCGTCTTG TCTGCCGCTC 
    GGCTTCATCG TCTTCCTGCC CGCTGTGCTG CTGCTGGTGG CGCCGCCGCT GCCTGCCGCC
    GACGCCGCGC ACGAGTTCAC CGTGTACCGC ATGCAGCAGT ACGACCTGCA GGGCCAGCCC
    TACGGCACAC GGAATGCAGT GCTGAACACG GAGGCGCGCA CGATGGCGGC GGAGGTGCTG
    AGCCGCCGCT GCGTGCTCAT GCGGCTACTG GACTTCTCCT ACGAGCAGTA CCAGAAGGCC
    CTGCGGCAGT CGGCGGGCGC CGTGGTCATC ATCCTGCCCA GGGCCATGGC CGCCGTGCCC
    CAGGACGTCG TCCGGCAATT CATGGAGATC GAGCCGGAGA TGCTGGCCAT GGAGACCGCC
    GTCCCCGTGT ACTTTGCCGT GGAGGACGAG GCCCTGCTGT CTATCTACAA GCAGACCCAG
    GCTGCCTCCG CCTCCCAGGG CTCCGCCTCT GCTGCTGAAG TACTGCTGCG CACGGCCACT
    GCCAACGGCT TCCAGATGGT CACCAGCGGG GTACAGAGCA AGGCCGTGAG TGACTGGCTG
    ATTGCCAGCG TGGAGGGGCG GCTGACGGGG CTGGGCGGAG AGGACCTTCC CACCATCGTC
    ATCGTGGCCC ACTACGACGC CTTTGGAGTG GCCCCCTGGC TGTCGCTGGG CGCGGACTCC
    AACGGGAGCG GCGTCTCTGT GCTGCTGGAG CTGGCACGCC TCTTCTCCCG GCTCTACACC
    TACAAGCGCA CGCACGCCGC CTACAACCTC CTGTTCTTTG CGTCTGGAGG AGGCAAGTTT
    AACTACCAGG GAACCAAGCG CTGGCTGGAA GACAACCTGG ACCACACAGA CTCCAGCCTG
    CTTCAGGACA ATGTGGCCTT CGTGCTGTGC CTGGACACCG TGGGCCGGGG CAGCAGCCTG
    CACCTGCACG TGTCCAAGCC GCCTCGGGAG GGCACCCTGC AGCACGCCTT CCTGCGGGAG
    CTGGAGACGG TGGCCGCGCA CCAGTTCCCT GAGGTACGGT TCTCCATGGT GCACAAGCGG
    ATCAACCTGG CGGAGGACGT GCTGGCCTGG GAGCACGAGC GCTTCGCCAT CCGCCGACTG
    CCCGCCTTCA CGCTGTCCCA CCTGGAGAGC CACCGTGACG GCCAGCGCAG CAGCATCATG
    GACGTGCGGT CCCGGGTGGA TTCTAAGACC CTGACCCGTA ACACGAGGAT CATTGCAGAG
    GCCCTGACTC GAGTCATCTA CAACCTGACA GAGAAGGGGA CACCCCCAGA CATGCCGGTG
    TTCACAGAGC AGATGCAGAT CCAGCAGGAG CAGCTGGACT CGGTGATGGA CTGGCTCACC
    AACCAGCCGC GGGCCGCGCA GCTGGTGGAC AAGGACAGCA CCTTCCTCAG CACGCTGGAG
    CACCACCTGA GCCGCTACCT GAAGGACGTG AAGCAGCACC ACGTCAAGGC TGACAAGCGG
    GACCCAGAGT TTGTCTTCTA CGACCAGCTG AAGCAAGTGA TGAATGCGTA CAGAGTCAAG
    CCGGCCGTCT TTGACCTGCT CCTGGCTGTT GGCATTGCTG CCTACCTCGG CATGGCCTAC
    GTGGCTGTCC AGCACTTCAG CCTCCTCTAC AAGACCGTCC AGAGGCTGCT CGTGAAGGCC
    AAGACACAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu51703
    Clone ID Related Accession (Same CDS sequence) NM_001321463.2 , NM_001321463.1
    Accession Version NM_001321463.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1689bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-31
    Organism Homo sapiens(human)
    Product nicalin isoform 2 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC011547.4, BU508470.1, BC025926.1, BG420915.1 and GD158011.1. On May 31, 2019 this sequence version replaced NM_001321463.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.2105963.1, SRR1803617.5351.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCTGGAGG AAGCGGGCGA GGTGCTGGAG AACATGCTGA AGGCGTCTTG TCTGCCGCTC 
    GGCTTCATCG TCTTCCTGCC CGCTGTGCTG CTGCTGGTGG CGCCGCCGCT GCCTGCCGCC
    GACGCCGCGC ACGAGTTCAC CGTGTACCGC ATGCAGCAGT ACGACCTGCA GGGCCAGCCC
    TACGGCACAC GGAATGCAGT GCTGAACACG GAGGCGCGCA CGATGGCGGC GGAGGTGCTG
    AGCCGCCGCT GCGTGCTCAT GCGGCTACTG GACTTCTCCT ACGAGCAGTA CCAGAAGGCC
    CTGCGGCAGT CGGCGGGCGC CGTGGTCATC ATCCTGCCCA GGGCCATGGC CGCCGTGCCC
    CAGGACGTCG TCCGGCAATT CATGGAGATC GAGCCGGAGA TGCTGGCCAT GGAGACCGCC
    GTCCCCGTGT ACTTTGCCGT GGAGGACGAG GCCCTGCTGT CTATCTACAA GCAGACCCAG
    GCTGCCTCCG CCTCCCAGGG CTCCGCCTCT GCTGCTGAAG TACTGCTGCG CACGGCCACT
    GCCAACGGCT TCCAGATGGT CACCAGCGGG GTACAGAGCA AGGCCGTGAG TGACTGGCTG
    ATTGCCAGCG TGGAGGGGCG GCTGACGGGG CTGGGCGGAG AGGACCTTCC CACCATCGTC
    ATCGTGGCCC ACTACGACGC CTTTGGAGTG GCCCCCTGGC TGTCGCTGGG CGCGGACTCC
    AACGGGAGCG GCGTCTCTGT GCTGCTGGAG CTGGCACGCC TCTTCTCCCG GCTCTACACC
    TACAAGCGCA CGCACGCCGC CTACAACCTC CTGTTCTTTG CGTCTGGAGG AGGCAAGTTT
    AACTACCAGG GAACCAAGCG CTGGCTGGAA GACAACCTGG ACCACACAGA CTCCAGCCTG
    CTTCAGGACA ATGTGGCCTT CGTGCTGTGC CTGGACACCG TGGGCCGGGG CAGCAGCCTG
    CACCTGCACG TGTCCAAGCC GCCTCGGGAG GGCACCCTGC AGCACGCCTT CCTGCGGGAG
    CTGGAGACGG TGGCCGCGCA CCAGTTCCCT GAGGTACGGT TCTCCATGGT GCACAAGCGG
    ATCAACCTGG CGGAGGACGT GCTGGCCTGG GAGCACGAGC GCTTCGCCAT CCGCCGACTG
    CCCGCCTTCA CGCTGTCCCA CCTGGAGAGC CACCGTGACG GCCAGCGCAG CAGCATCATG
    GACGTGCGGT CCCGGGTGGA TTCTAAGACC CTGACCCGTA ACACGAGGAT CATTGCAGAG
    GCCCTGACTC GAGTCATCTA CAACCTGACA GAGAAGGGGA CACCCCCAGA CATGCCGGTG
    TTCACAGAGC AGATGATCCA GCAGGAGCAG CTGGACTCGG TGATGGACTG GCTCACCAAC
    CAGCCGCGGG CCGCGCAGCT GGTGGACAAG GACAGCACCT TCCTCAGCAC GCTGGAGCAC
    CACCTGAGCC GCTACCTGAA GGACGTGAAG CAGCACCACG TCAAGGCTGA CAAGCGGGAC
    CCAGAGTTTG TCTTCTACGA CCAGCTGAAG CAAGTGATGA ATGCGTACAG AGTCAAGCCG
    GCCGTCTTTG ACCTGCTCCT GGCTGTTGGC ATTGCTGCCT ACCTCGGCAT GGCCTACGTG
    GCTGTCCAGC ACTTCAGCCT CCTCTACAAG ACCGTCCAGA GGCTGCTCGT GAAGGCCAAG
    ACACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001308392.1
    CDS102..1790
    Translation

    Target ORF information:

    RefSeq Version NM_001321463.2
    Organism Homo sapiens(human)
    Definition Homo sapiens nicalin (NCLN), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001321463.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCTGGAGG AAGCGGGCGA GGTGCTGGAG AACATGCTGA AGGCGTCTTG TCTGCCGCTC 
    GGCTTCATCG TCTTCCTGCC CGCTGTGCTG CTGCTGGTGG CGCCGCCGCT GCCTGCCGCC
    GACGCCGCGC ACGAGTTCAC CGTGTACCGC ATGCAGCAGT ACGACCTGCA GGGCCAGCCC
    TACGGCACAC GGAATGCAGT GCTGAACACG GAGGCGCGCA CGATGGCGGC GGAGGTGCTG
    AGCCGCCGCT GCGTGCTCAT GCGGCTACTG GACTTCTCCT ACGAGCAGTA CCAGAAGGCC
    CTGCGGCAGT CGGCGGGCGC CGTGGTCATC ATCCTGCCCA GGGCCATGGC CGCCGTGCCC
    CAGGACGTCG TCCGGCAATT CATGGAGATC GAGCCGGAGA TGCTGGCCAT GGAGACCGCC
    GTCCCCGTGT ACTTTGCCGT GGAGGACGAG GCCCTGCTGT CTATCTACAA GCAGACCCAG
    GCTGCCTCCG CCTCCCAGGG CTCCGCCTCT GCTGCTGAAG TACTGCTGCG CACGGCCACT
    GCCAACGGCT TCCAGATGGT CACCAGCGGG GTACAGAGCA AGGCCGTGAG TGACTGGCTG
    ATTGCCAGCG TGGAGGGGCG GCTGACGGGG CTGGGCGGAG AGGACCTTCC CACCATCGTC
    ATCGTGGCCC ACTACGACGC CTTTGGAGTG GCCCCCTGGC TGTCGCTGGG CGCGGACTCC
    AACGGGAGCG GCGTCTCTGT GCTGCTGGAG CTGGCACGCC TCTTCTCCCG GCTCTACACC
    TACAAGCGCA CGCACGCCGC CTACAACCTC CTGTTCTTTG CGTCTGGAGG AGGCAAGTTT
    AACTACCAGG GAACCAAGCG CTGGCTGGAA GACAACCTGG ACCACACAGA CTCCAGCCTG
    CTTCAGGACA ATGTGGCCTT CGTGCTGTGC CTGGACACCG TGGGCCGGGG CAGCAGCCTG
    CACCTGCACG TGTCCAAGCC GCCTCGGGAG GGCACCCTGC AGCACGCCTT CCTGCGGGAG
    CTGGAGACGG TGGCCGCGCA CCAGTTCCCT GAGGTACGGT TCTCCATGGT GCACAAGCGG
    ATCAACCTGG CGGAGGACGT GCTGGCCTGG GAGCACGAGC GCTTCGCCAT CCGCCGACTG
    CCCGCCTTCA CGCTGTCCCA CCTGGAGAGC CACCGTGACG GCCAGCGCAG CAGCATCATG
    GACGTGCGGT CCCGGGTGGA TTCTAAGACC CTGACCCGTA ACACGAGGAT CATTGCAGAG
    GCCCTGACTC GAGTCATCTA CAACCTGACA GAGAAGGGGA CACCCCCAGA CATGCCGGTG
    TTCACAGAGC AGATGATCCA GCAGGAGCAG CTGGACTCGG TGATGGACTG GCTCACCAAC
    CAGCCGCGGG CCGCGCAGCT GGTGGACAAG GACAGCACCT TCCTCAGCAC GCTGGAGCAC
    CACCTGAGCC GCTACCTGAA GGACGTGAAG CAGCACCACG TCAAGGCTGA CAAGCGGGAC
    CCAGAGTTTG TCTTCTACGA CCAGCTGAAG CAAGTGATGA ATGCGTACAG AGTCAAGCCG
    GCCGTCTTTG ACCTGCTCCT GGCTGTTGGC ATTGCTGCCT ACCTCGGCAT GGCCTACGTG
    GCTGTCCAGC ACTTCAGCCT CCTCTACAAG ACCGTCCAGA GGCTGCTCGT GAAGGCCAAG
    ACACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu51703
    Clone ID Related Accession (Same CDS sequence) NM_001321463.2 , NM_001321463.1
    Accession Version NM_001321463.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1689bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-24
    Organism Homo sapiens(human)
    Product nicalin isoform 2 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC011547.4, BU508470.1, BC025926.1, BG420915.1 and GD158011.1. On Mar 25, 2016 this sequence version replaced XM_005259596.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.311668.1, BC025926.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCTGGAGG AAGCGGGCGA GGTGCTGGAG AACATGCTGA AGGCGTCTTG TCTGCCGCTC 
    GGCTTCATCG TCTTCCTGCC CGCTGTGCTG CTGCTGGTGG CGCCGCCGCT GCCTGCCGCC
    GACGCCGCGC ACGAGTTCAC CGTGTACCGC ATGCAGCAGT ACGACCTGCA GGGCCAGCCC
    TACGGCACAC GGAATGCAGT GCTGAACACG GAGGCGCGCA CGATGGCGGC GGAGGTGCTG
    AGCCGCCGCT GCGTGCTCAT GCGGCTACTG GACTTCTCCT ACGAGCAGTA CCAGAAGGCC
    CTGCGGCAGT CGGCGGGCGC CGTGGTCATC ATCCTGCCCA GGGCCATGGC CGCCGTGCCC
    CAGGACGTCG TCCGGCAATT CATGGAGATC GAGCCGGAGA TGCTGGCCAT GGAGACCGCC
    GTCCCCGTGT ACTTTGCCGT GGAGGACGAG GCCCTGCTGT CTATCTACAA GCAGACCCAG
    GCTGCCTCCG CCTCCCAGGG CTCCGCCTCT GCTGCTGAAG TACTGCTGCG CACGGCCACT
    GCCAACGGCT TCCAGATGGT CACCAGCGGG GTACAGAGCA AGGCCGTGAG TGACTGGCTG
    ATTGCCAGCG TGGAGGGGCG GCTGACGGGG CTGGGCGGAG AGGACCTTCC CACCATCGTC
    ATCGTGGCCC ACTACGACGC CTTTGGAGTG GCCCCCTGGC TGTCGCTGGG CGCGGACTCC
    AACGGGAGCG GCGTCTCTGT GCTGCTGGAG CTGGCACGCC TCTTCTCCCG GCTCTACACC
    TACAAGCGCA CGCACGCCGC CTACAACCTC CTGTTCTTTG CGTCTGGAGG AGGCAAGTTT
    AACTACCAGG GAACCAAGCG CTGGCTGGAA GACAACCTGG ACCACACAGA CTCCAGCCTG
    CTTCAGGACA ATGTGGCCTT CGTGCTGTGC CTGGACACCG TGGGCCGGGG CAGCAGCCTG
    CACCTGCACG TGTCCAAGCC GCCTCGGGAG GGCACCCTGC AGCACGCCTT CCTGCGGGAG
    CTGGAGACGG TGGCCGCGCA CCAGTTCCCT GAGGTACGGT TCTCCATGGT GCACAAGCGG
    ATCAACCTGG CGGAGGACGT GCTGGCCTGG GAGCACGAGC GCTTCGCCAT CCGCCGACTG
    CCCGCCTTCA CGCTGTCCCA CCTGGAGAGC CACCGTGACG GCCAGCGCAG CAGCATCATG
    GACGTGCGGT CCCGGGTGGA TTCTAAGACC CTGACCCGTA ACACGAGGAT CATTGCAGAG
    GCCCTGACTC GAGTCATCTA CAACCTGACA GAGAAGGGGA CACCCCCAGA CATGCCGGTG
    TTCACAGAGC AGATGATCCA GCAGGAGCAG CTGGACTCGG TGATGGACTG GCTCACCAAC
    CAGCCGCGGG CCGCGCAGCT GGTGGACAAG GACAGCACCT TCCTCAGCAC GCTGGAGCAC
    CACCTGAGCC GCTACCTGAA GGACGTGAAG CAGCACCACG TCAAGGCTGA CAAGCGGGAC
    CCAGAGTTTG TCTTCTACGA CCAGCTGAAG CAAGTGATGA ATGCGTACAG AGTCAAGCCG
    GCCGTCTTTG ACCTGCTCCT GGCTGTTGGC ATTGCTGCCT ACCTCGGCAT GGCCTACGTG
    GCTGTCCAGC ACTTCAGCCT CCTCTACAAG ACCGTCCAGA GGCTGCTCGT GAAGGCCAAG
    ACACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001308392.1
    CDS155..1843
    Translation

    Target ORF information:

    RefSeq Version NM_001321463.1
    Organism Homo sapiens(human)
    Definition Homo sapiens nicalin (NCLN), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001321463.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCTGGAGG AAGCGGGCGA GGTGCTGGAG AACATGCTGA AGGCGTCTTG TCTGCCGCTC 
    GGCTTCATCG TCTTCCTGCC CGCTGTGCTG CTGCTGGTGG CGCCGCCGCT GCCTGCCGCC
    GACGCCGCGC ACGAGTTCAC CGTGTACCGC ATGCAGCAGT ACGACCTGCA GGGCCAGCCC
    TACGGCACAC GGAATGCAGT GCTGAACACG GAGGCGCGCA CGATGGCGGC GGAGGTGCTG
    AGCCGCCGCT GCGTGCTCAT GCGGCTACTG GACTTCTCCT ACGAGCAGTA CCAGAAGGCC
    CTGCGGCAGT CGGCGGGCGC CGTGGTCATC ATCCTGCCCA GGGCCATGGC CGCCGTGCCC
    CAGGACGTCG TCCGGCAATT CATGGAGATC GAGCCGGAGA TGCTGGCCAT GGAGACCGCC
    GTCCCCGTGT ACTTTGCCGT GGAGGACGAG GCCCTGCTGT CTATCTACAA GCAGACCCAG
    GCTGCCTCCG CCTCCCAGGG CTCCGCCTCT GCTGCTGAAG TACTGCTGCG CACGGCCACT
    GCCAACGGCT TCCAGATGGT CACCAGCGGG GTACAGAGCA AGGCCGTGAG TGACTGGCTG
    ATTGCCAGCG TGGAGGGGCG GCTGACGGGG CTGGGCGGAG AGGACCTTCC CACCATCGTC
    ATCGTGGCCC ACTACGACGC CTTTGGAGTG GCCCCCTGGC TGTCGCTGGG CGCGGACTCC
    AACGGGAGCG GCGTCTCTGT GCTGCTGGAG CTGGCACGCC TCTTCTCCCG GCTCTACACC
    TACAAGCGCA CGCACGCCGC CTACAACCTC CTGTTCTTTG CGTCTGGAGG AGGCAAGTTT
    AACTACCAGG GAACCAAGCG CTGGCTGGAA GACAACCTGG ACCACACAGA CTCCAGCCTG
    CTTCAGGACA ATGTGGCCTT CGTGCTGTGC CTGGACACCG TGGGCCGGGG CAGCAGCCTG
    CACCTGCACG TGTCCAAGCC GCCTCGGGAG GGCACCCTGC AGCACGCCTT CCTGCGGGAG
    CTGGAGACGG TGGCCGCGCA CCAGTTCCCT GAGGTACGGT TCTCCATGGT GCACAAGCGG
    ATCAACCTGG CGGAGGACGT GCTGGCCTGG GAGCACGAGC GCTTCGCCAT CCGCCGACTG
    CCCGCCTTCA CGCTGTCCCA CCTGGAGAGC CACCGTGACG GCCAGCGCAG CAGCATCATG
    GACGTGCGGT CCCGGGTGGA TTCTAAGACC CTGACCCGTA ACACGAGGAT CATTGCAGAG
    GCCCTGACTC GAGTCATCTA CAACCTGACA GAGAAGGGGA CACCCCCAGA CATGCCGGTG
    TTCACAGAGC AGATGATCCA GCAGGAGCAG CTGGACTCGG TGATGGACTG GCTCACCAAC
    CAGCCGCGGG CCGCGCAGCT GGTGGACAAG GACAGCACCT TCCTCAGCAC GCTGGAGCAC
    CACCTGAGCC GCTACCTGAA GGACGTGAAG CAGCACCACG TCAAGGCTGA CAAGCGGGAC
    CCAGAGTTTG TCTTCTACGA CCAGCTGAAG CAAGTGATGA ATGCGTACAG AGTCAAGCCG
    GCCGTCTTTG ACCTGCTCCT GGCTGTTGGC ATTGCTGCCT ACCTCGGCAT GGCCTACGTG
    GCTGTCCAGC ACTTCAGCCT CCTCTACAAG ACCGTCCAGA GGCTGCTCGT GAAGGCCAAG
    ACACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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