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NLRC4 cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol NLRC4
Entrez Gene ID 58484
Full Name NLR family CARD domain containing 4
Synonyms AIFEC,CARD12,CLAN,CLAN1,CLANA,CLANB,CLANC,CLAND,CLR2.1,FCAS4,IPAF
General protein information
Preferred Names

NLR family CARD domain containing 4

Names

NLR family CARD domain-containing protein 4
CARD, LRR, and NACHT-containing protein
ICE-protease activating factor
NOD-like receptor C4
caspase recruitment domain family, member 12
caspase recruitment domain-containing protein 12
nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 4
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

2

2p22.3
Summary This gene encodes a member of the caspase recruitment domain-containing NLR family. Family members play essential roles in innate immune response to a wide range of pathogenic organisms, tissue damage and other cellular stresses. Mutations in this gene result in autoinflammation with infantile enterocolitis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014].
Disorder MIM:

606831

Disorder Html: Autoinflammation with infantile enterocolitis, 616050 (3); ?Familial cold autoinflammatory syndrome 4, 616115 (3)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_021209.4 NP_067032.3 NLR family CARD domain-containing protein 4 isoform a
NM_001199139.1 NP_001186068.1 NLR family CARD domain-containing protein 4 isoform a
XM_017004619.1 XP_016860108.1 NLR family CARD domain-containing protein 4 isoform X1
NM_001199138.1 NP_001186067.1 NLR family CARD domain-containing protein 4 isoform a
NM_001302504.1 NP_001289433.1 NLR family CARD domain-containing protein 4 isoform b
NM_001199138.2 NP_001186067.1 NLR family CARD domain-containing protein 4 isoform a

Pathways from BioSystems


Homologies


NLRC4 XP_001105818.2
Bos taurus (cattle) NLRC4 NP_001179252.2
Homo sapiens (human) NLRC4 NP_001186067.1
Pan troglodytes (chimpanzee) NLRC4 XP_515390.2
Xenopus tropicalis (tropical clawed frog) nlrc4 XP_004914732.1
Mus musculus (house mouse) Nlrc4 NP_001028539.1
Rattus norvegicus (Norway rat) Nlrc4 XP_003750157.2

Gene Ontology

The following NLRC4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NLRC4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu70975 NM_001302504.1
Latest version!		 Homo sapiens NLR family CARD domain containing 4 (NLRC4), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu89747 XM_017004619.1
Latest version!		 Homo sapiens NLR family CARD domain containing 4 (NLRC4), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $391.30
$559.00
OHu16706 NM_021209.4
Latest version!		 Homo sapiens NLR family CARD domain containing 4 (NLRC4), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 16 $384.30
$549.00
OHu16706 NM_001199138.2
Latest version!		 Homo sapiens NLR family CARD domain containing 4 (NLRC4), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 16 $384.30
$549.00
OHu16706 NM_001199139.1
Latest version!		 Homo sapiens NLR family CARD domain containing 4 (NLRC4), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 16 $384.30
$549.00
View Old Accession Versions >>
OHu16706 NM_001199138.1 Homo sapiens NLR family CARD domain containing 4 (NLRC4), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 16 $384.30
$549.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu70975
Clone ID Related Accession (Same CDS sequence) NM_001302504.1
Accession Version NM_001302504.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1080bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-27
Organism Homo sapiens(human)
Product NLR family CARD domain-containing protein 4 isoform b
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL121653.2, AY027788.1 and AW337918.1. Transcript Variant: This variant (4) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. It encodes isoform b, which is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY027788.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGAATTTCA TAAAGGACAA TAGCCGAGCC CTTATTCAAA GAATGGGAAT GACTGTTATA 
AAGCAAATCA CAGATGACCT ATTTGTATGG AATGTTCTGA ATCGCGAAGA AGTAAACATC 
ATTTGCTGCG AGAAGGTGGA GCAGGATGCT GCTAGAGGGA TCATTCACAT GATTTTGAAA 
AAGGGTTCAG AGTCCTGTAA CCTCTTTCTT AAATCCCTTA AGGAGTGGAA CTATCCTCTA 
TTTCAGGACT TGAATGGACA AAGTGGTCTG ACTGACAGCT TGGGTAACTT GAAGAACCTT 
ACAAAGCTCA TAATGGATAA CATAAAGATG AATGAAGAAG ATGCTATAAA ACTAGCTGAA 
GGCCTGAAAA ACCTGAAGAA GATGTGTTTA TTTCATTTGA CCCACTTGTC TGACATTGGA 
GAGGGAATGG ATTACATAGT CAAGTCTCTG TCAAGTGAAC CCTGTGACCT TGAAGAAATT 
CAATTAGTCT CCTGCTGCTT GTCTGCAAAT GCAGTGAAAA TCCTAGCTCA GAATCTTCAC 
AATTTGGTCA AACTGAGCAT TCTTGATTTA TCAGAAAATT ACCTGGAAAA AGATGGAAAT 
GAAGCTCTTC ATGAACTGAT CGACAGGATG AACGTGCTAG AACAGCTCAC CGCACTGATG 
CTGCCCTGGG GCTGTGACGT GCAAGGCAGC CTGAGCAGCC TGTTGAAACA TTTGGAGGAG 
GTCCCACAAC TCGTCAAGCT TGGGTTGAAA AACTGGAGAC TCACAGATAC AGAGATTAGA 
ATTTTAGGTG CATTTTTTGG AAAGAACCCT CTGAAAAACT TCCAGCAGTT GAATTTGGCG 
GGAAATCGTG TGAGCAGTGA TGGATGGCTT GCCTTCATGG GTGTATTTGA GAATCTTAAG 
CAATTAGTGT TTTTTGACTT TAGTACTAAA GAATTTCTAC CTGATCCAGC ATTAGTCAGA 
AAACTTAGCC AAGTGTTATC CAAGTTAACT TTTCTGCAAG AAGCTAGGCT TGTTGGGTGG 
CAATTTGATG ATGATGATCT CAGTGTTATT ACAGGTGCTT TTAAACTAGT AACTGCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001289433.1
CDS276..1355
Translation

Target ORF information:

RefSeq Version NM_001302504.1
Organism Homo sapiens(human)
Definition Homo sapiens NLR family CARD domain containing 4 (NLRC4), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001302504.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGAATTTCA TAAAGGACAA TAGCCGAGCC CTTATTCAAA GAATGGGAAT GACTGTTATA 
AAGCAAATCA CAGATGACCT ATTTGTATGG AATGTTCTGA ATCGCGAAGA AGTAAACATC 
ATTTGCTGCG AGAAGGTGGA GCAGGATGCT GCTAGAGGGA TCATTCACAT GATTTTGAAA 
AAGGGTTCAG AGTCCTGTAA CCTCTTTCTT AAATCCCTTA AGGAGTGGAA CTATCCTCTA 
TTTCAGGACT TGAATGGACA AAGTGGTCTG ACTGACAGCT TGGGTAACTT GAAGAACCTT 
ACAAAGCTCA TAATGGATAA CATAAAGATG AATGAAGAAG ATGCTATAAA ACTAGCTGAA 
GGCCTGAAAA ACCTGAAGAA GATGTGTTTA TTTCATTTGA CCCACTTGTC TGACATTGGA 
GAGGGAATGG ATTACATAGT CAAGTCTCTG TCAAGTGAAC CCTGTGACCT TGAAGAAATT 
CAATTAGTCT CCTGCTGCTT GTCTGCAAAT GCAGTGAAAA TCCTAGCTCA GAATCTTCAC 
AATTTGGTCA AACTGAGCAT TCTTGATTTA TCAGAAAATT ACCTGGAAAA AGATGGAAAT 
GAAGCTCTTC ATGAACTGAT CGACAGGATG AACGTGCTAG AACAGCTCAC CGCACTGATG 
CTGCCCTGGG GCTGTGACGT GCAAGGCAGC CTGAGCAGCC TGTTGAAACA TTTGGAGGAG 
GTCCCACAAC TCGTCAAGCT TGGGTTGAAA AACTGGAGAC TCACAGATAC AGAGATTAGA 
ATTTTAGGTG CATTTTTTGG AAAGAACCCT CTGAAAAACT TCCAGCAGTT GAATTTGGCG 
GGAAATCGTG TGAGCAGTGA TGGATGGCTT GCCTTCATGG GTGTATTTGA GAATCTTAAG 
CAATTAGTGT TTTTTGACTT TAGTACTAAA GAATTTCTAC CTGATCCAGC ATTAGTCAGA 
AAACTTAGCC AAGTGTTATC CAAGTTAACT TTTCTGCAAG AAGCTAGGCT TGTTGGGTGG 
CAATTTGATG ATGATGATCT CAGTGTTATT ACAGGTGCTT TTAAACTAGT AACTGCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu89747
Clone ID Related Accession (Same CDS sequence) XM_017004619.1
Accession Version XM_017004619.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2367bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product NLR family CARD domain-containing protein 4 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGAATTTCA TAAAGGACAA TAGCCGAGCC CTTATTCAAA GAATGGGAAT GACTGTTATA 
AAGCAAATCA CAGATGACCT ATTTGTATGG AATGTTCTGA ATCGCGAAGA AGTAAACATC 
ATTTGCTGCG AGAAGGTGGA GCAGGATGCT GCTAGAGGGA TCATTCACAT GATTTTGAAA 
AAGGGTTCAG AGTCCTGTAA CCTCTTTCTT AAATCCCTTA AGGAGTGGAA CTATCCTCTA 
TTTCAGGACT TGAATGGACA AAGTCTTTTT CATCAGACAT CAGAAGGAGA CTTGGACGAT 
TTGGCTCAGG ATTTAAAGGA CTTGTACCAT ACCCCATCTT TTCTGAACTT TTATCCCCTT 
GGTGAAGATA TTGACATTAT TTTTAACTTG AAAAGCACCT TCACAGAACC TGTCCTGTGG 
AGGAAGGACC AACACCATCA CCGCGTGGAG CAGCTGACCC TGAATGGCCT CCTGCAGGCT 
CTTCAGAGCC CCTGCATCAT TGAAGGGGAA TCTGGCAAAG GCAAGTCCAC TCTGCTGCAG 
CGAATTGCCA TGCTCTGGGG CTCCGGAAAG TGCAAGGCTC TGACCAAGTT CAAATTCGTC 
TTCTTCCTCC GTCTCAGCAG GGCCCAGGGT GGACTTTTTG AAACCCTCTG TGATCAACTC 
CTGGATATAC CTGGCACAAT CAGGAAGCAG ACATTCATGG CCATGCTGCT GAAGCTGCGG 
CAGAGGGTTC TTTTCCTTCT TGATGGCTAC AATGAATTCA AGCCCCAGAA CTGCCCAGAA 
ATCGAAGCCC TGATAAAGGA AAACCACCGC TTCAAGAACA TGGTCATCGT CACCACTACC 
ACTGAGTGCC TGAGGCACAT ACGGCAGTTT GGTGCCCTGA CTGCTGAGGT GGGGGATATG 
ACAGAAGACA GCGCCCAGGC TCTCATCCGA GAAGTGCTGA TCAAGGAGCT TGCTGAAGGC 
TTGTTGCTCC AAATTCAGAA ATCCAGGTGC TTGAGGAATC TCATGAAGAC CCCTCTCTTT 
GTGGTCATCA CTTGTGCAAT CCAGATGGGT GAAAGTGAGT TCCACTCTCA CACACAAACA 
ACGCTGTTCC ATACCTTCTA TGATCTGTTG ATACAGAAAA ACAAACACAA ACATAAAGGT 
GTGGCTGCAA GTGACTTCAT TCGGAGCCTG GACCACTGTG GAGACCTAGC TCTGGAGGGT 
GTGTTCTCCC ACAAGTTTGA TTTCGAACTG CAGGATGTGT CCAGCGTGAA TGAGGATGTC 
CTGCTGACAA CTGGGCTCCT CTGTAAATAT ACAGCTCAAA GGTTCAAGCC AAAGTATAAA 
TTCTTTCACA AGTCATTCCA GGAGTACACA GCAGGACGAA GACTCAGCAG TTTATTGACG 
TCTCATGAGC CAGAGGAGGT GACCAAGGGG AATGGTTACT TGCAGAAAAT GGTTTCCATT 
TCGGACATTA CATCCACTTA TAGCAGCCTG CTCCGGTACA CCTGTGGGTC ATCTGTGGAA 
GCCACCAGGG CTGTTATGAA GCACCTCGCA GCAGTGTATC AACACGGCTG CCTTCTCGGA 
CTTTCCATCG CCAAGAGGCC TCTCTGGAGA CAGGAATCTT TGCAAAGTGT GAAAAACACC 
ACTGAGCAAG AAATTCTGAA AGCCATAAAC ATCAATTCCT TTGTAGAGTG TGGCATCCAT 
TTATATCAAG AGAGTACATC CAAATCAGCC CTGAGCCAAG AATTTGAAGC TTTCTTTCAA 
GGTAAAAGCT TATATATCAA CTCAGGGAAC ATCCCCGATT ACTTATTTGA CTTCTTTGAA 
CATTTGCCCA ATTGTGCAAG TGCCCTGGAC TTCATTAAAC TGGACTTTTA TGGGGGAGCT 
ATGGCTTCAT GGGAAAAGGC TGCAGAAGAC ACAGGTGGAA TCCACATGGA AGAGGCCCCA 
GAAACCTACA TTCCCAGCAG GGCTGTATCT TTGTTCTTCA ACTGGAAGCA GGAATTCAGG 
ACTCTGGAGG TCACACTCCG GGATTTCAGC AAGTTGAATA AGCAAGATAT CAGATATCTG 
GGGAAAATAT TCAGCTCTGC CACAAGCCTC AGGCTGCAAA TAAAGAGATG TGCTGGTGTG 
GCTGGAAGCC TCAGTTTGGT CCTCAGCACC TGTAAGAACA TTTATTCTCT CATGGTGGAA 
GCCAGTCCCC TCACCATAGA AGATGAGAGG CACATCACAT CTGTAACAAA CCTGAAAACC 
TTGAGTATTC ATGACCTACA GAATCAACGG CTGCCGGGTG GTCTGACTGA CAGCTTGGGT 
AACTTGAAGA ACCTTACAAA GCTCATAATG GATAACATAA AGATGAATGA AGAAGATGCT 
ATAAAACTAG ACAACTCTAG CCTATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016860108.1
CDS262..2628
Translation

Target ORF information:

RefSeq Version XM_017004619.1
Organism Homo sapiens(human)
Definition Homo sapiens NLR family CARD domain containing 4 (NLRC4), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017004619.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGAATTTCA TAAAGGACAA TAGCCGAGCC CTTATTCAAA GAATGGGAAT GACTGTTATA 
AAGCAAATCA CAGATGACCT ATTTGTATGG AATGTTCTGA ATCGCGAAGA AGTAAACATC 
ATTTGCTGCG AGAAGGTGGA GCAGGATGCT GCTAGAGGGA TCATTCACAT GATTTTGAAA 
AAGGGTTCAG AGTCCTGTAA CCTCTTTCTT AAATCCCTTA AGGAGTGGAA CTATCCTCTA 
TTTCAGGACT TGAATGGACA AAGTCTTTTT CATCAGACAT CAGAAGGAGA CTTGGACGAT 
TTGGCTCAGG ATTTAAAGGA CTTGTACCAT ACCCCATCTT TTCTGAACTT TTATCCCCTT 
GGTGAAGATA TTGACATTAT TTTTAACTTG AAAAGCACCT TCACAGAACC TGTCCTGTGG 
AGGAAGGACC AACACCATCA CCGCGTGGAG CAGCTGACCC TGAATGGCCT CCTGCAGGCT 
CTTCAGAGCC CCTGCATCAT TGAAGGGGAA TCTGGCAAAG GCAAGTCCAC TCTGCTGCAG 
CGAATTGCCA TGCTCTGGGG CTCCGGAAAG TGCAAGGCTC TGACCAAGTT CAAATTCGTC 
TTCTTCCTCC GTCTCAGCAG GGCCCAGGGT GGACTTTTTG AAACCCTCTG TGATCAACTC 
CTGGATATAC CTGGCACAAT CAGGAAGCAG ACATTCATGG CCATGCTGCT GAAGCTGCGG 
CAGAGGGTTC TTTTCCTTCT TGATGGCTAC AATGAATTCA AGCCCCAGAA CTGCCCAGAA 
ATCGAAGCCC TGATAAAGGA AAACCACCGC TTCAAGAACA TGGTCATCGT CACCACTACC 
ACTGAGTGCC TGAGGCACAT ACGGCAGTTT GGTGCCCTGA CTGCTGAGGT GGGGGATATG 
ACAGAAGACA GCGCCCAGGC TCTCATCCGA GAAGTGCTGA TCAAGGAGCT TGCTGAAGGC 
TTGTTGCTCC AAATTCAGAA ATCCAGGTGC TTGAGGAATC TCATGAAGAC CCCTCTCTTT 
GTGGTCATCA CTTGTGCAAT CCAGATGGGT GAAAGTGAGT TCCACTCTCA CACACAAACA 
ACGCTGTTCC ATACCTTCTA TGATCTGTTG ATACAGAAAA ACAAACACAA ACATAAAGGT 
GTGGCTGCAA GTGACTTCAT TCGGAGCCTG GACCACTGTG GAGACCTAGC TCTGGAGGGT 
GTGTTCTCCC ACAAGTTTGA TTTCGAACTG CAGGATGTGT CCAGCGTGAA TGAGGATGTC 
CTGCTGACAA CTGGGCTCCT CTGTAAATAT ACAGCTCAAA GGTTCAAGCC AAAGTATAAA 
TTCTTTCACA AGTCATTCCA GGAGTACACA GCAGGACGAA GACTCAGCAG TTTATTGACG 
TCTCATGAGC CAGAGGAGGT GACCAAGGGG AATGGTTACT TGCAGAAAAT GGTTTCCATT 
TCGGACATTA CATCCACTTA TAGCAGCCTG CTCCGGTACA CCTGTGGGTC ATCTGTGGAA 
GCCACCAGGG CTGTTATGAA GCACCTCGCA GCAGTGTATC AACACGGCTG CCTTCTCGGA 
CTTTCCATCG CCAAGAGGCC TCTCTGGAGA CAGGAATCTT TGCAAAGTGT GAAAAACACC 
ACTGAGCAAG AAATTCTGAA AGCCATAAAC ATCAATTCCT TTGTAGAGTG TGGCATCCAT 
TTATATCAAG AGAGTACATC CAAATCAGCC CTGAGCCAAG AATTTGAAGC TTTCTTTCAA 
GGTAAAAGCT TATATATCAA CTCAGGGAAC ATCCCCGATT ACTTATTTGA CTTCTTTGAA 
CATTTGCCCA ATTGTGCAAG TGCCCTGGAC TTCATTAAAC TGGACTTTTA TGGGGGAGCT 
ATGGCTTCAT GGGAAAAGGC TGCAGAAGAC ACAGGTGGAA TCCACATGGA AGAGGCCCCA 
GAAACCTACA TTCCCAGCAG GGCTGTATCT TTGTTCTTCA ACTGGAAGCA GGAATTCAGG 
ACTCTGGAGG TCACACTCCG GGATTTCAGC AAGTTGAATA AGCAAGATAT CAGATATCTG 
GGGAAAATAT TCAGCTCTGC CACAAGCCTC AGGCTGCAAA TAAAGAGATG TGCTGGTGTG 
GCTGGAAGCC TCAGTTTGGT CCTCAGCACC TGTAAGAACA TTTATTCTCT CATGGTGGAA 
GCCAGTCCCC TCACCATAGA AGATGAGAGG CACATCACAT CTGTAACAAA CCTGAAAACC 
TTGAGTATTC ATGACCTACA GAATCAACGG CTGCCGGGTG GTCTGACTGA CAGCTTGGGT 
AACTTGAAGA ACCTTACAAA GCTCATAATG GATAACATAA AGATGAATGA AGAAGATGCT 
ATAAAACTAG ACAACTCTAG CCTATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16706
Clone ID Related Accession (Same CDS sequence) NM_021209.4 , NM_001199139.1 , NM_001199138.1 , NM_001199138.2
Accession Version NM_021209.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3075bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-27
Organism Homo sapiens(human)
Product NLR family CARD domain-containing protein 4 isoform a
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DN998613.1, BC031555.1 and AW337918.1. On Nov 23, 2010 this sequence version replaced NM_021209.3. Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY027787.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142670 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
ATGAATTTCA TAAAGGACAA TAGCCGAGCC CTTATTCAAA GAATGGGAAT GACTGTTATA 
AAGCAAATCA CAGATGACCT ATTTGTATGG AATGTTCTGA ATCGCGAAGA AGTAAACATC 
ATTTGCTGCG AGAAGGTGGA GCAGGATGCT GCTAGAGGGA TCATTCACAT GATTTTGAAA 
AAGGGTTCAG AGTCCTGTAA CCTCTTTCTT AAATCCCTTA AGGAGTGGAA CTATCCTCTA 
TTTCAGGACT TGAATGGACA AAGTCTTTTT CATCAGACAT CAGAAGGAGA CTTGGACGAT 
TTGGCTCAGG ATTTAAAGGA CTTGTACCAT ACCCCATCTT TTCTGAACTT TTATCCCCTT 
GGTGAAGATA TTGACATTAT TTTTAACTTG AAAAGCACCT TCACAGAACC TGTCCTGTGG 
AGGAAGGACC AACACCATCA CCGCGTGGAG CAGCTGACCC TGAATGGCCT CCTGCAGGCT 
CTTCAGAGCC CCTGCATCAT TGAAGGGGAA TCTGGCAAAG GCAAGTCCAC TCTGCTGCAG 
CGAATTGCCA TGCTCTGGGG CTCCGGAAAG TGCAAGGCTC TGACCAAGTT CAAATTCGTC 
TTCTTCCTCC GTCTCAGCAG GGCCCAGGGT GGACTTTTTG AAACCCTCTG TGATCAACTC 
CTGGATATAC CTGGCACAAT CAGGAAGCAG ACATTCATGG CCATGCTGCT GAAGCTGCGG 
CAGAGGGTTC TTTTCCTTCT TGATGGCTAC AATGAATTCA AGCCCCAGAA CTGCCCAGAA 
ATCGAAGCCC TGATAAAGGA AAACCACCGC TTCAAGAACA TGGTCATCGT CACCACTACC 
ACTGAGTGCC TGAGGCACAT ACGGCAGTTT GGTGCCCTGA CTGCTGAGGT GGGGGATATG 
ACAGAAGACA GCGCCCAGGC TCTCATCCGA GAAGTGCTGA TCAAGGAGCT TGCTGAAGGC 
TTGTTGCTCC AAATTCAGAA ATCCAGGTGC TTGAGGAATC TCATGAAGAC CCCTCTCTTT 
GTGGTCATCA CTTGTGCAAT CCAGATGGGT GAAAGTGAGT TCCACTCTCA CACACAAACA 
ACGCTGTTCC ATACCTTCTA TGATCTGTTG ATACAGAAAA ACAAACACAA ACATAAAGGT 
GTGGCTGCAA GTGACTTCAT TCGGAGCCTG GACCACTGTG GAGACCTAGC TCTGGAGGGT 
GTGTTCTCCC ACAAGTTTGA TTTCGAACTG CAGGATGTGT CCAGCGTGAA TGAGGATGTC 
CTGCTGACAA CTGGGCTCCT CTGTAAATAT ACAGCTCAAA GGTTCAAGCC AAAGTATAAA 
TTCTTTCACA AGTCATTCCA GGAGTACACA GCAGGACGAA GACTCAGCAG TTTATTGACG 
TCTCATGAGC CAGAGGAGGT GACCAAGGGG AATGGTTACT TGCAGAAAAT GGTTTCCATT 
TCGGACATTA CATCCACTTA TAGCAGCCTG CTCCGGTACA CCTGTGGGTC ATCTGTGGAA 
GCCACCAGGG CTGTTATGAA GCACCTCGCA GCAGTGTATC AACACGGCTG CCTTCTCGGA 
CTTTCCATCG CCAAGAGGCC TCTCTGGAGA CAGGAATCTT TGCAAAGTGT GAAAAACACC 
ACTGAGCAAG AAATTCTGAA AGCCATAAAC ATCAATTCCT TTGTAGAGTG TGGCATCCAT 
TTATATCAAG AGAGTACATC CAAATCAGCC CTGAGCCAAG AATTTGAAGC TTTCTTTCAA 
GGTAAAAGCT TATATATCAA CTCAGGGAAC ATCCCCGATT ACTTATTTGA CTTCTTTGAA 
CATTTGCCCA ATTGTGCAAG TGCCCTGGAC TTCATTAAAC TGGACTTTTA TGGGGGAGCT 
ATGGCTTCAT GGGAAAAGGC TGCAGAAGAC ACAGGTGGAA TCCACATGGA AGAGGCCCCA 
GAAACCTACA TTCCCAGCAG GGCTGTATCT TTGTTCTTCA ACTGGAAGCA GGAATTCAGG 
ACTCTGGAGG TCACACTCCG GGATTTCAGC AAGTTGAATA AGCAAGATAT CAGATATCTG 
GGGAAAATAT TCAGCTCTGC CACAAGCCTC AGGCTGCAAA TAAAGAGATG TGCTGGTGTG 
GCTGGAAGCC TCAGTTTGGT CCTCAGCACC TGTAAGAACA TTTATTCTCT CATGGTGGAA 
GCCAGTCCCC TCACCATAGA AGATGAGAGG CACATCACAT CTGTAACAAA CCTGAAAACC 
TTGAGTATTC ATGACCTACA GAATCAACGG CTGCCGGGTG GTCTGACTGA CAGCTTGGGT 
AACTTGAAGA ACCTTACAAA GCTCATAATG GATAACATAA AGATGAATGA AGAAGATGCT 
ATAAAACTAG CTGAAGGCCT GAAAAACCTG AAGAAGATGT GTTTATTTCA TTTGACCCAC 
TTGTCTGACA TTGGAGAGGG AATGGATTAC ATAGTCAAGT CTCTGTCAAG TGAACCCTGT 
GACCTTGAAG AAATTCAATT AGTCTCCTGC TGCTTGTCTG CAAATGCAGT GAAAATCCTA 
GCTCAGAATC TTCACAATTT GGTCAAACTG AGCATTCTTG ATTTATCAGA AAATTACCTG 
GAAAAAGATG GAAATGAAGC TCTTCATGAA CTGATCGACA GGATGAACGT GCTAGAACAG 
CTCACCGCAC TGATGCTGCC CTGGGGCTGT GACGTGCAAG GCAGCCTGAG CAGCCTGTTG 
AAACATTTGG AGGAGGTCCC ACAACTCGTC AAGCTTGGGT TGAAAAACTG GAGACTCACA 
GATACAGAGA TTAGAATTTT AGGTGCATTT TTTGGAAAGA ACCCTCTGAA AAACTTCCAG 
CAGTTGAATT TGGCGGGAAA TCGTGTGAGC AGTGATGGAT GGCTTGCCTT CATGGGTGTA 
TTTGAGAATC TTAAGCAATT AGTGTTTTTT GACTTTAGTA CTAAAGAATT TCTACCTGAT 
CCAGCATTAG TCAGAAAACT TAGCCAAGTG TTATCCAAGT TAACTTTTCT GCAAGAAGCT 
AGGCTTGTTG GGTGGCAATT TGATGATGAT GATCTCAGTG TTATTACAGG TGCTTTTAAA 
CTAGTAACTG CTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_067032.3
CDS276..3350
Translation

Target ORF information:

RefSeq Version NM_021209.4
Organism Homo sapiens(human)
Definition Homo sapiens NLR family CARD domain containing 4 (NLRC4), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_021209.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
ATGAATTTCA TAAAGGACAA TAGCCGAGCC CTTATTCAAA GAATGGGAAT GACTGTTATA 
AAGCAAATCA CAGATGACCT ATTTGTATGG AATGTTCTGA ATCGCGAAGA AGTAAACATC 
ATTTGCTGCG AGAAGGTGGA GCAGGATGCT GCTAGAGGGA TCATTCACAT GATTTTGAAA 
AAGGGTTCAG AGTCCTGTAA CCTCTTTCTT AAATCCCTTA AGGAGTGGAA CTATCCTCTA 
TTTCAGGACT TGAATGGACA AAGTCTTTTT CATCAGACAT CAGAAGGAGA CTTGGACGAT 
TTGGCTCAGG ATTTAAAGGA CTTGTACCAT ACCCCATCTT TTCTGAACTT TTATCCCCTT 
GGTGAAGATA TTGACATTAT TTTTAACTTG AAAAGCACCT TCACAGAACC TGTCCTGTGG 
AGGAAGGACC AACACCATCA CCGCGTGGAG CAGCTGACCC TGAATGGCCT CCTGCAGGCT 
CTTCAGAGCC CCTGCATCAT TGAAGGGGAA TCTGGCAAAG GCAAGTCCAC TCTGCTGCAG 
CGAATTGCCA TGCTCTGGGG CTCCGGAAAG TGCAAGGCTC TGACCAAGTT CAAATTCGTC 
TTCTTCCTCC GTCTCAGCAG GGCCCAGGGT GGACTTTTTG AAACCCTCTG TGATCAACTC 
CTGGATATAC CTGGCACAAT CAGGAAGCAG ACATTCATGG CCATGCTGCT GAAGCTGCGG 
CAGAGGGTTC TTTTCCTTCT TGATGGCTAC AATGAATTCA AGCCCCAGAA CTGCCCAGAA 
ATCGAAGCCC TGATAAAGGA AAACCACCGC TTCAAGAACA TGGTCATCGT CACCACTACC 
ACTGAGTGCC TGAGGCACAT ACGGCAGTTT GGTGCCCTGA CTGCTGAGGT GGGGGATATG 
ACAGAAGACA GCGCCCAGGC TCTCATCCGA GAAGTGCTGA TCAAGGAGCT TGCTGAAGGC 
TTGTTGCTCC AAATTCAGAA ATCCAGGTGC TTGAGGAATC TCATGAAGAC CCCTCTCTTT 
GTGGTCATCA CTTGTGCAAT CCAGATGGGT GAAAGTGAGT TCCACTCTCA CACACAAACA 
ACGCTGTTCC ATACCTTCTA TGATCTGTTG ATACAGAAAA ACAAACACAA ACATAAAGGT 
GTGGCTGCAA GTGACTTCAT TCGGAGCCTG GACCACTGTG GAGACCTAGC TCTGGAGGGT 
GTGTTCTCCC ACAAGTTTGA TTTCGAACTG CAGGATGTGT CCAGCGTGAA TGAGGATGTC 
CTGCTGACAA CTGGGCTCCT CTGTAAATAT ACAGCTCAAA GGTTCAAGCC AAAGTATAAA 
TTCTTTCACA AGTCATTCCA GGAGTACACA GCAGGACGAA GACTCAGCAG TTTATTGACG 
TCTCATGAGC CAGAGGAGGT GACCAAGGGG AATGGTTACT TGCAGAAAAT GGTTTCCATT 
TCGGACATTA CATCCACTTA TAGCAGCCTG CTCCGGTACA CCTGTGGGTC ATCTGTGGAA 
GCCACCAGGG CTGTTATGAA GCACCTCGCA GCAGTGTATC AACACGGCTG CCTTCTCGGA 
CTTTCCATCG CCAAGAGGCC TCTCTGGAGA CAGGAATCTT TGCAAAGTGT GAAAAACACC 
ACTGAGCAAG AAATTCTGAA AGCCATAAAC ATCAATTCCT TTGTAGAGTG TGGCATCCAT 
TTATATCAAG AGAGTACATC CAAATCAGCC CTGAGCCAAG AATTTGAAGC TTTCTTTCAA 
GGTAAAAGCT TATATATCAA CTCAGGGAAC ATCCCCGATT ACTTATTTGA CTTCTTTGAA 
CATTTGCCCA ATTGTGCAAG TGCCCTGGAC TTCATTAAAC TGGACTTTTA TGGGGGAGCT 
ATGGCTTCAT GGGAAAAGGC TGCAGAAGAC ACAGGTGGAA TCCACATGGA AGAGGCCCCA 
GAAACCTACA TTCCCAGCAG GGCTGTATCT TTGTTCTTCA ACTGGAAGCA GGAATTCAGG 
ACTCTGGAGG TCACACTCCG GGATTTCAGC AAGTTGAATA AGCAAGATAT CAGATATCTG 
GGGAAAATAT TCAGCTCTGC CACAAGCCTC AGGCTGCAAA TAAAGAGATG TGCTGGTGTG 
GCTGGAAGCC TCAGTTTGGT CCTCAGCACC TGTAAGAACA TTTATTCTCT CATGGTGGAA 
GCCAGTCCCC TCACCATAGA AGATGAGAGG CACATCACAT CTGTAACAAA CCTGAAAACC 
TTGAGTATTC ATGACCTACA GAATCAACGG CTGCCGGGTG GTCTGACTGA CAGCTTGGGT 
AACTTGAAGA ACCTTACAAA GCTCATAATG GATAACATAA AGATGAATGA AGAAGATGCT 
ATAAAACTAG CTGAAGGCCT GAAAAACCTG AAGAAGATGT GTTTATTTCA TTTGACCCAC 
TTGTCTGACA TTGGAGAGGG AATGGATTAC ATAGTCAAGT CTCTGTCAAG TGAACCCTGT 
GACCTTGAAG AAATTCAATT AGTCTCCTGC TGCTTGTCTG CAAATGCAGT GAAAATCCTA 
GCTCAGAATC TTCACAATTT GGTCAAACTG AGCATTCTTG ATTTATCAGA AAATTACCTG 
GAAAAAGATG GAAATGAAGC TCTTCATGAA CTGATCGACA GGATGAACGT GCTAGAACAG 
CTCACCGCAC TGATGCTGCC CTGGGGCTGT GACGTGCAAG GCAGCCTGAG CAGCCTGTTG 
AAACATTTGG AGGAGGTCCC ACAACTCGTC AAGCTTGGGT TGAAAAACTG GAGACTCACA 
GATACAGAGA TTAGAATTTT AGGTGCATTT TTTGGAAAGA ACCCTCTGAA AAACTTCCAG 
CAGTTGAATT TGGCGGGAAA TCGTGTGAGC AGTGATGGAT GGCTTGCCTT CATGGGTGTA 
TTTGAGAATC TTAAGCAATT AGTGTTTTTT GACTTTAGTA CTAAAGAATT TCTACCTGAT 
CCAGCATTAG TCAGAAAACT TAGCCAAGTG TTATCCAAGT TAACTTTTCT GCAAGAAGCT 
AGGCTTGTTG GGTGGCAATT TGATGATGAT GATCTCAGTG TTATTACAGG TGCTTTTAAA 
CTAGTAACTG CTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16706
Clone ID Related Accession (Same CDS sequence) NM_021209.4 , NM_001199139.1 , NM_001199138.1 , NM_001199138.2
Accession Version NM_001199139.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3075bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-27
Organism Homo sapiens(human)
Product NLR family CARD domain-containing protein 4 isoform a
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY035391.1 and AW337918.1. Transcript Variant: This variant (3) has an alternate splice site in the 5' UTR and encodes the same protein, as compared to variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY035391.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
ATGAATTTCA TAAAGGACAA TAGCCGAGCC CTTATTCAAA GAATGGGAAT GACTGTTATA 
AAGCAAATCA CAGATGACCT ATTTGTATGG AATGTTCTGA ATCGCGAAGA AGTAAACATC 
ATTTGCTGCG AGAAGGTGGA GCAGGATGCT GCTAGAGGGA TCATTCACAT GATTTTGAAA 
AAGGGTTCAG AGTCCTGTAA CCTCTTTCTT AAATCCCTTA AGGAGTGGAA CTATCCTCTA 
TTTCAGGACT TGAATGGACA AAGTCTTTTT CATCAGACAT CAGAAGGAGA CTTGGACGAT 
TTGGCTCAGG ATTTAAAGGA CTTGTACCAT ACCCCATCTT TTCTGAACTT TTATCCCCTT 
GGTGAAGATA TTGACATTAT TTTTAACTTG AAAAGCACCT TCACAGAACC TGTCCTGTGG 
AGGAAGGACC AACACCATCA CCGCGTGGAG CAGCTGACCC TGAATGGCCT CCTGCAGGCT 
CTTCAGAGCC CCTGCATCAT TGAAGGGGAA TCTGGCAAAG GCAAGTCCAC TCTGCTGCAG 
CGAATTGCCA TGCTCTGGGG CTCCGGAAAG TGCAAGGCTC TGACCAAGTT CAAATTCGTC 
TTCTTCCTCC GTCTCAGCAG GGCCCAGGGT GGACTTTTTG AAACCCTCTG TGATCAACTC 
CTGGATATAC CTGGCACAAT CAGGAAGCAG ACATTCATGG CCATGCTGCT GAAGCTGCGG 
CAGAGGGTTC TTTTCCTTCT TGATGGCTAC AATGAATTCA AGCCCCAGAA CTGCCCAGAA 
ATCGAAGCCC TGATAAAGGA AAACCACCGC TTCAAGAACA TGGTCATCGT CACCACTACC 
ACTGAGTGCC TGAGGCACAT ACGGCAGTTT GGTGCCCTGA CTGCTGAGGT GGGGGATATG 
ACAGAAGACA GCGCCCAGGC TCTCATCCGA GAAGTGCTGA TCAAGGAGCT TGCTGAAGGC 
TTGTTGCTCC AAATTCAGAA ATCCAGGTGC TTGAGGAATC TCATGAAGAC CCCTCTCTTT 
GTGGTCATCA CTTGTGCAAT CCAGATGGGT GAAAGTGAGT TCCACTCTCA CACACAAACA 
ACGCTGTTCC ATACCTTCTA TGATCTGTTG ATACAGAAAA ACAAACACAA ACATAAAGGT 
GTGGCTGCAA GTGACTTCAT TCGGAGCCTG GACCACTGTG GAGACCTAGC TCTGGAGGGT 
GTGTTCTCCC ACAAGTTTGA TTTCGAACTG CAGGATGTGT CCAGCGTGAA TGAGGATGTC 
CTGCTGACAA CTGGGCTCCT CTGTAAATAT ACAGCTCAAA GGTTCAAGCC AAAGTATAAA 
TTCTTTCACA AGTCATTCCA GGAGTACACA GCAGGACGAA GACTCAGCAG TTTATTGACG 
TCTCATGAGC CAGAGGAGGT GACCAAGGGG AATGGTTACT TGCAGAAAAT GGTTTCCATT 
TCGGACATTA CATCCACTTA TAGCAGCCTG CTCCGGTACA CCTGTGGGTC ATCTGTGGAA 
GCCACCAGGG CTGTTATGAA GCACCTCGCA GCAGTGTATC AACACGGCTG CCTTCTCGGA 
CTTTCCATCG CCAAGAGGCC TCTCTGGAGA CAGGAATCTT TGCAAAGTGT GAAAAACACC 
ACTGAGCAAG AAATTCTGAA AGCCATAAAC ATCAATTCCT TTGTAGAGTG TGGCATCCAT 
TTATATCAAG AGAGTACATC CAAATCAGCC CTGAGCCAAG AATTTGAAGC TTTCTTTCAA 
GGTAAAAGCT TATATATCAA CTCAGGGAAC ATCCCCGATT ACTTATTTGA CTTCTTTGAA 
CATTTGCCCA ATTGTGCAAG TGCCCTGGAC TTCATTAAAC TGGACTTTTA TGGGGGAGCT 
ATGGCTTCAT GGGAAAAGGC TGCAGAAGAC ACAGGTGGAA TCCACATGGA AGAGGCCCCA 
GAAACCTACA TTCCCAGCAG GGCTGTATCT TTGTTCTTCA ACTGGAAGCA GGAATTCAGG 
ACTCTGGAGG TCACACTCCG GGATTTCAGC AAGTTGAATA AGCAAGATAT CAGATATCTG 
GGGAAAATAT TCAGCTCTGC CACAAGCCTC AGGCTGCAAA TAAAGAGATG TGCTGGTGTG 
GCTGGAAGCC TCAGTTTGGT CCTCAGCACC TGTAAGAACA TTTATTCTCT CATGGTGGAA 
GCCAGTCCCC TCACCATAGA AGATGAGAGG CACATCACAT CTGTAACAAA CCTGAAAACC 
TTGAGTATTC ATGACCTACA GAATCAACGG CTGCCGGGTG GTCTGACTGA CAGCTTGGGT 
AACTTGAAGA ACCTTACAAA GCTCATAATG GATAACATAA AGATGAATGA AGAAGATGCT 
ATAAAACTAG CTGAAGGCCT GAAAAACCTG AAGAAGATGT GTTTATTTCA TTTGACCCAC 
TTGTCTGACA TTGGAGAGGG AATGGATTAC ATAGTCAAGT CTCTGTCAAG TGAACCCTGT 
GACCTTGAAG AAATTCAATT AGTCTCCTGC TGCTTGTCTG CAAATGCAGT GAAAATCCTA 
GCTCAGAATC TTCACAATTT GGTCAAACTG AGCATTCTTG ATTTATCAGA AAATTACCTG 
GAAAAAGATG GAAATGAAGC TCTTCATGAA CTGATCGACA GGATGAACGT GCTAGAACAG 
CTCACCGCAC TGATGCTGCC CTGGGGCTGT GACGTGCAAG GCAGCCTGAG CAGCCTGTTG 
AAACATTTGG AGGAGGTCCC ACAACTCGTC AAGCTTGGGT TGAAAAACTG GAGACTCACA 
GATACAGAGA TTAGAATTTT AGGTGCATTT TTTGGAAAGA ACCCTCTGAA AAACTTCCAG 
CAGTTGAATT TGGCGGGAAA TCGTGTGAGC AGTGATGGAT GGCTTGCCTT CATGGGTGTA 
TTTGAGAATC TTAAGCAATT AGTGTTTTTT GACTTTAGTA CTAAAGAATT TCTACCTGAT 
CCAGCATTAG TCAGAAAACT TAGCCAAGTG TTATCCAAGT TAACTTTTCT GCAAGAAGCT 
AGGCTTGTTG GGTGGCAATT TGATGATGAT GATCTCAGTG TTATTACAGG TGCTTTTAAA 
CTAGTAACTG CTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001186068.1
CDS145..3219
Translation

Target ORF information:

RefSeq Version NM_001199139.1
Organism Homo sapiens(human)
Definition Homo sapiens NLR family CARD domain containing 4 (NLRC4), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001199139.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
ATGAATTTCA TAAAGGACAA TAGCCGAGCC CTTATTCAAA GAATGGGAAT GACTGTTATA 
AAGCAAATCA CAGATGACCT ATTTGTATGG AATGTTCTGA ATCGCGAAGA AGTAAACATC 
ATTTGCTGCG AGAAGGTGGA GCAGGATGCT GCTAGAGGGA TCATTCACAT GATTTTGAAA 
AAGGGTTCAG AGTCCTGTAA CCTCTTTCTT AAATCCCTTA AGGAGTGGAA CTATCCTCTA 
TTTCAGGACT TGAATGGACA AAGTCTTTTT CATCAGACAT CAGAAGGAGA CTTGGACGAT 
TTGGCTCAGG ATTTAAAGGA CTTGTACCAT ACCCCATCTT TTCTGAACTT TTATCCCCTT 
GGTGAAGATA TTGACATTAT TTTTAACTTG AAAAGCACCT TCACAGAACC TGTCCTGTGG 
AGGAAGGACC AACACCATCA CCGCGTGGAG CAGCTGACCC TGAATGGCCT CCTGCAGGCT 
CTTCAGAGCC CCTGCATCAT TGAAGGGGAA TCTGGCAAAG GCAAGTCCAC TCTGCTGCAG 
CGAATTGCCA TGCTCTGGGG CTCCGGAAAG TGCAAGGCTC TGACCAAGTT CAAATTCGTC 
TTCTTCCTCC GTCTCAGCAG GGCCCAGGGT GGACTTTTTG AAACCCTCTG TGATCAACTC 
CTGGATATAC CTGGCACAAT CAGGAAGCAG ACATTCATGG CCATGCTGCT GAAGCTGCGG 
CAGAGGGTTC TTTTCCTTCT TGATGGCTAC AATGAATTCA AGCCCCAGAA CTGCCCAGAA 
ATCGAAGCCC TGATAAAGGA AAACCACCGC TTCAAGAACA TGGTCATCGT CACCACTACC 
ACTGAGTGCC TGAGGCACAT ACGGCAGTTT GGTGCCCTGA CTGCTGAGGT GGGGGATATG 
ACAGAAGACA GCGCCCAGGC TCTCATCCGA GAAGTGCTGA TCAAGGAGCT TGCTGAAGGC 
TTGTTGCTCC AAATTCAGAA ATCCAGGTGC TTGAGGAATC TCATGAAGAC CCCTCTCTTT 
GTGGTCATCA CTTGTGCAAT CCAGATGGGT GAAAGTGAGT TCCACTCTCA CACACAAACA 
ACGCTGTTCC ATACCTTCTA TGATCTGTTG ATACAGAAAA ACAAACACAA ACATAAAGGT 
GTGGCTGCAA GTGACTTCAT TCGGAGCCTG GACCACTGTG GAGACCTAGC TCTGGAGGGT 
GTGTTCTCCC ACAAGTTTGA TTTCGAACTG CAGGATGTGT CCAGCGTGAA TGAGGATGTC 
CTGCTGACAA CTGGGCTCCT CTGTAAATAT ACAGCTCAAA GGTTCAAGCC AAAGTATAAA 
TTCTTTCACA AGTCATTCCA GGAGTACACA GCAGGACGAA GACTCAGCAG TTTATTGACG 
TCTCATGAGC CAGAGGAGGT GACCAAGGGG AATGGTTACT TGCAGAAAAT GGTTTCCATT 
TCGGACATTA CATCCACTTA TAGCAGCCTG CTCCGGTACA CCTGTGGGTC ATCTGTGGAA 
GCCACCAGGG CTGTTATGAA GCACCTCGCA GCAGTGTATC AACACGGCTG CCTTCTCGGA 
CTTTCCATCG CCAAGAGGCC TCTCTGGAGA CAGGAATCTT TGCAAAGTGT GAAAAACACC 
ACTGAGCAAG AAATTCTGAA AGCCATAAAC ATCAATTCCT TTGTAGAGTG TGGCATCCAT 
TTATATCAAG AGAGTACATC CAAATCAGCC CTGAGCCAAG AATTTGAAGC TTTCTTTCAA 
GGTAAAAGCT TATATATCAA CTCAGGGAAC ATCCCCGATT ACTTATTTGA CTTCTTTGAA 
CATTTGCCCA ATTGTGCAAG TGCCCTGGAC TTCATTAAAC TGGACTTTTA TGGGGGAGCT 
ATGGCTTCAT GGGAAAAGGC TGCAGAAGAC ACAGGTGGAA TCCACATGGA AGAGGCCCCA 
GAAACCTACA TTCCCAGCAG GGCTGTATCT TTGTTCTTCA ACTGGAAGCA GGAATTCAGG 
ACTCTGGAGG TCACACTCCG GGATTTCAGC AAGTTGAATA AGCAAGATAT CAGATATCTG 
GGGAAAATAT TCAGCTCTGC CACAAGCCTC AGGCTGCAAA TAAAGAGATG TGCTGGTGTG 
GCTGGAAGCC TCAGTTTGGT CCTCAGCACC TGTAAGAACA TTTATTCTCT CATGGTGGAA 
GCCAGTCCCC TCACCATAGA AGATGAGAGG CACATCACAT CTGTAACAAA CCTGAAAACC 
TTGAGTATTC ATGACCTACA GAATCAACGG CTGCCGGGTG GTCTGACTGA CAGCTTGGGT 
AACTTGAAGA ACCTTACAAA GCTCATAATG GATAACATAA AGATGAATGA AGAAGATGCT 
ATAAAACTAG CTGAAGGCCT GAAAAACCTG AAGAAGATGT GTTTATTTCA TTTGACCCAC 
TTGTCTGACA TTGGAGAGGG AATGGATTAC ATAGTCAAGT CTCTGTCAAG TGAACCCTGT 
GACCTTGAAG AAATTCAATT AGTCTCCTGC TGCTTGTCTG CAAATGCAGT GAAAATCCTA 
GCTCAGAATC TTCACAATTT GGTCAAACTG AGCATTCTTG ATTTATCAGA AAATTACCTG 
GAAAAAGATG GAAATGAAGC TCTTCATGAA CTGATCGACA GGATGAACGT GCTAGAACAG 
CTCACCGCAC TGATGCTGCC CTGGGGCTGT GACGTGCAAG GCAGCCTGAG CAGCCTGTTG 
AAACATTTGG AGGAGGTCCC ACAACTCGTC AAGCTTGGGT TGAAAAACTG GAGACTCACA 
GATACAGAGA TTAGAATTTT AGGTGCATTT TTTGGAAAGA ACCCTCTGAA AAACTTCCAG 
CAGTTGAATT TGGCGGGAAA TCGTGTGAGC AGTGATGGAT GGCTTGCCTT CATGGGTGTA 
TTTGAGAATC TTAAGCAATT AGTGTTTTTT GACTTTAGTA CTAAAGAATT TCTACCTGAT 
CCAGCATTAG TCAGAAAACT TAGCCAAGTG TTATCCAAGT TAACTTTTCT GCAAGAAGCT 
AGGCTTGTTG GGTGGCAATT TGATGATGAT GATCTCAGTG TTATTACAGG TGCTTTTAAA 
CTAGTAACTG CTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16706
Clone ID Related Accession (Same CDS sequence) NM_021209.4 , NM_001199139.1 , NM_001199138.1 , NM_001199138.2
Accession Version NM_001199138.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3075bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-12-17
Organism Homo sapiens(human)
Product NLR family CARD domain-containing protein 4 isoform a
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK095467.1, BC031555.1 and AW337918.1. Transcript Variant: This variant (2) has an alternate 5' UTR exon and encodes the same protein, as compared to variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK095467.1, SRR1660803.29064.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
ATGAATTTCA TAAAGGACAA TAGCCGAGCC CTTATTCAAA GAATGGGAAT GACTGTTATA 
AAGCAAATCA CAGATGACCT ATTTGTATGG AATGTTCTGA ATCGCGAAGA AGTAAACATC 
ATTTGCTGCG AGAAGGTGGA GCAGGATGCT GCTAGAGGGA TCATTCACAT GATTTTGAAA 
AAGGGTTCAG AGTCCTGTAA CCTCTTTCTT AAATCCCTTA AGGAGTGGAA CTATCCTCTA 
TTTCAGGACT TGAATGGACA AAGTCTTTTT CATCAGACAT CAGAAGGAGA CTTGGACGAT 
TTGGCTCAGG ATTTAAAGGA CTTGTACCAT ACCCCATCTT TTCTGAACTT TTATCCCCTT 
GGTGAAGATA TTGACATTAT TTTTAACTTG AAAAGCACCT TCACAGAACC TGTCCTGTGG 
AGGAAGGACC AACACCATCA CCGCGTGGAG CAGCTGACCC TGAATGGCCT CCTGCAGGCT 
CTTCAGAGCC CCTGCATCAT TGAAGGGGAA TCTGGCAAAG GCAAGTCCAC TCTGCTGCAG 
CGAATTGCCA TGCTCTGGGG CTCCGGAAAG TGCAAGGCTC TGACCAAGTT CAAATTCGTC 
TTCTTCCTCC GTCTCAGCAG GGCCCAGGGT GGACTTTTTG AAACCCTCTG TGATCAACTC 
CTGGATATAC CTGGCACAAT CAGGAAGCAG ACATTCATGG CCATGCTGCT GAAGCTGCGG 
CAGAGGGTTC TTTTCCTTCT TGATGGCTAC AATGAATTCA AGCCCCAGAA CTGCCCAGAA 
ATCGAAGCCC TGATAAAGGA AAACCACCGC TTCAAGAACA TGGTCATCGT CACCACTACC 
ACTGAGTGCC TGAGGCACAT ACGGCAGTTT GGTGCCCTGA CTGCTGAGGT GGGGGATATG 
ACAGAAGACA GCGCCCAGGC TCTCATCCGA GAAGTGCTGA TCAAGGAGCT TGCTGAAGGC 
TTGTTGCTCC AAATTCAGAA ATCCAGGTGC TTGAGGAATC TCATGAAGAC CCCTCTCTTT 
GTGGTCATCA CTTGTGCAAT CCAGATGGGT GAAAGTGAGT TCCACTCTCA CACACAAACA 
ACGCTGTTCC ATACCTTCTA TGATCTGTTG ATACAGAAAA ACAAACACAA ACATAAAGGT 
GTGGCTGCAA GTGACTTCAT TCGGAGCCTG GACCACTGTG GAGACCTAGC TCTGGAGGGT 
GTGTTCTCCC ACAAGTTTGA TTTCGAACTG CAGGATGTGT CCAGCGTGAA TGAGGATGTC 
CTGCTGACAA CTGGGCTCCT CTGTAAATAT ACAGCTCAAA GGTTCAAGCC AAAGTATAAA 
TTCTTTCACA AGTCATTCCA GGAGTACACA GCAGGACGAA GACTCAGCAG TTTATTGACG 
TCTCATGAGC CAGAGGAGGT GACCAAGGGG AATGGTTACT TGCAGAAAAT GGTTTCCATT 
TCGGACATTA CATCCACTTA TAGCAGCCTG CTCCGGTACA CCTGTGGGTC ATCTGTGGAA 
GCCACCAGGG CTGTTATGAA GCACCTCGCA GCAGTGTATC AACACGGCTG CCTTCTCGGA 
CTTTCCATCG CCAAGAGGCC TCTCTGGAGA CAGGAATCTT TGCAAAGTGT GAAAAACACC 
ACTGAGCAAG AAATTCTGAA AGCCATAAAC ATCAATTCCT TTGTAGAGTG TGGCATCCAT 
TTATATCAAG AGAGTACATC CAAATCAGCC CTGAGCCAAG AATTTGAAGC TTTCTTTCAA 
GGTAAAAGCT TATATATCAA CTCAGGGAAC ATCCCCGATT ACTTATTTGA CTTCTTTGAA 
CATTTGCCCA ATTGTGCAAG TGCCCTGGAC TTCATTAAAC TGGACTTTTA TGGGGGAGCT 
ATGGCTTCAT GGGAAAAGGC TGCAGAAGAC ACAGGTGGAA TCCACATGGA AGAGGCCCCA 
GAAACCTACA TTCCCAGCAG GGCTGTATCT TTGTTCTTCA ACTGGAAGCA GGAATTCAGG 
ACTCTGGAGG TCACACTCCG GGATTTCAGC AAGTTGAATA AGCAAGATAT CAGATATCTG 
GGGAAAATAT TCAGCTCTGC CACAAGCCTC AGGCTGCAAA TAAAGAGATG TGCTGGTGTG 
GCTGGAAGCC TCAGTTTGGT CCTCAGCACC TGTAAGAACA TTTATTCTCT CATGGTGGAA 
GCCAGTCCCC TCACCATAGA AGATGAGAGG CACATCACAT CTGTAACAAA CCTGAAAACC 
TTGAGTATTC ATGACCTACA GAATCAACGG CTGCCGGGTG GTCTGACTGA CAGCTTGGGT 
AACTTGAAGA ACCTTACAAA GCTCATAATG GATAACATAA AGATGAATGA AGAAGATGCT 
ATAAAACTAG CTGAAGGCCT GAAAAACCTG AAGAAGATGT GTTTATTTCA TTTGACCCAC 
TTGTCTGACA TTGGAGAGGG AATGGATTAC ATAGTCAAGT CTCTGTCAAG TGAACCCTGT 
GACCTTGAAG AAATTCAATT AGTCTCCTGC TGCTTGTCTG CAAATGCAGT GAAAATCCTA 
GCTCAGAATC TTCACAATTT GGTCAAACTG AGCATTCTTG ATTTATCAGA AAATTACCTG 
GAAAAAGATG GAAATGAAGC TCTTCATGAA CTGATCGACA GGATGAACGT GCTAGAACAG 
CTCACCGCAC TGATGCTGCC CTGGGGCTGT GACGTGCAAG GCAGCCTGAG CAGCCTGTTG 
AAACATTTGG AGGAGGTCCC ACAACTCGTC AAGCTTGGGT TGAAAAACTG GAGACTCACA 
GATACAGAGA TTAGAATTTT AGGTGCATTT TTTGGAAAGA ACCCTCTGAA AAACTTCCAG 
CAGTTGAATT TGGCGGGAAA TCGTGTGAGC AGTGATGGAT GGCTTGCCTT CATGGGTGTA 
TTTGAGAATC TTAAGCAATT AGTGTTTTTT GACTTTAGTA CTAAAGAATT TCTACCTGAT 
CCAGCATTAG TCAGAAAACT TAGCCAAGTG TTATCCAAGT TAACTTTTCT GCAAGAAGCT 
AGGCTTGTTG GGTGGCAATT TGATGATGAT GATCTCAGTG TTATTACAGG TGCTTTTAAA 
CTAGTAACTG CTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001186067.1
CDS262..3336
Translation

Target ORF information:

RefSeq Version NM_001199138.1
Organism Homo sapiens(human)
Definition Homo sapiens NLR family CARD domain containing 4 (NLRC4), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001199138.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
ATGAATTTCA TAAAGGACAA TAGCCGAGCC CTTATTCAAA GAATGGGAAT GACTGTTATA 
AAGCAAATCA CAGATGACCT ATTTGTATGG AATGTTCTGA ATCGCGAAGA AGTAAACATC 
ATTTGCTGCG AGAAGGTGGA GCAGGATGCT GCTAGAGGGA TCATTCACAT GATTTTGAAA 
AAGGGTTCAG AGTCCTGTAA CCTCTTTCTT AAATCCCTTA AGGAGTGGAA CTATCCTCTA 
TTTCAGGACT TGAATGGACA AAGTCTTTTT CATCAGACAT CAGAAGGAGA CTTGGACGAT 
TTGGCTCAGG ATTTAAAGGA CTTGTACCAT ACCCCATCTT TTCTGAACTT TTATCCCCTT 
GGTGAAGATA TTGACATTAT TTTTAACTTG AAAAGCACCT TCACAGAACC TGTCCTGTGG 
AGGAAGGACC AACACCATCA CCGCGTGGAG CAGCTGACCC TGAATGGCCT CCTGCAGGCT 
CTTCAGAGCC CCTGCATCAT TGAAGGGGAA TCTGGCAAAG GCAAGTCCAC TCTGCTGCAG 
CGAATTGCCA TGCTCTGGGG CTCCGGAAAG TGCAAGGCTC TGACCAAGTT CAAATTCGTC 
TTCTTCCTCC GTCTCAGCAG GGCCCAGGGT GGACTTTTTG AAACCCTCTG TGATCAACTC 
CTGGATATAC CTGGCACAAT CAGGAAGCAG ACATTCATGG CCATGCTGCT GAAGCTGCGG 
CAGAGGGTTC TTTTCCTTCT TGATGGCTAC AATGAATTCA AGCCCCAGAA CTGCCCAGAA 
ATCGAAGCCC TGATAAAGGA AAACCACCGC TTCAAGAACA TGGTCATCGT CACCACTACC 
ACTGAGTGCC TGAGGCACAT ACGGCAGTTT GGTGCCCTGA CTGCTGAGGT GGGGGATATG 
ACAGAAGACA GCGCCCAGGC TCTCATCCGA GAAGTGCTGA TCAAGGAGCT TGCTGAAGGC 
TTGTTGCTCC AAATTCAGAA ATCCAGGTGC TTGAGGAATC TCATGAAGAC CCCTCTCTTT 
GTGGTCATCA CTTGTGCAAT CCAGATGGGT GAAAGTGAGT TCCACTCTCA CACACAAACA 
ACGCTGTTCC ATACCTTCTA TGATCTGTTG ATACAGAAAA ACAAACACAA ACATAAAGGT 
GTGGCTGCAA GTGACTTCAT TCGGAGCCTG GACCACTGTG GAGACCTAGC TCTGGAGGGT 
GTGTTCTCCC ACAAGTTTGA TTTCGAACTG CAGGATGTGT CCAGCGTGAA TGAGGATGTC 
CTGCTGACAA CTGGGCTCCT CTGTAAATAT ACAGCTCAAA GGTTCAAGCC AAAGTATAAA 
TTCTTTCACA AGTCATTCCA GGAGTACACA GCAGGACGAA GACTCAGCAG TTTATTGACG 
TCTCATGAGC CAGAGGAGGT GACCAAGGGG AATGGTTACT TGCAGAAAAT GGTTTCCATT 
TCGGACATTA CATCCACTTA TAGCAGCCTG CTCCGGTACA CCTGTGGGTC ATCTGTGGAA 
GCCACCAGGG CTGTTATGAA GCACCTCGCA GCAGTGTATC AACACGGCTG CCTTCTCGGA 
CTTTCCATCG CCAAGAGGCC TCTCTGGAGA CAGGAATCTT TGCAAAGTGT GAAAAACACC 
ACTGAGCAAG AAATTCTGAA AGCCATAAAC ATCAATTCCT TTGTAGAGTG TGGCATCCAT 
TTATATCAAG AGAGTACATC CAAATCAGCC CTGAGCCAAG AATTTGAAGC TTTCTTTCAA 
GGTAAAAGCT TATATATCAA CTCAGGGAAC ATCCCCGATT ACTTATTTGA CTTCTTTGAA 
CATTTGCCCA ATTGTGCAAG TGCCCTGGAC TTCATTAAAC TGGACTTTTA TGGGGGAGCT 
ATGGCTTCAT GGGAAAAGGC TGCAGAAGAC ACAGGTGGAA TCCACATGGA AGAGGCCCCA 
GAAACCTACA TTCCCAGCAG GGCTGTATCT TTGTTCTTCA ACTGGAAGCA GGAATTCAGG 
ACTCTGGAGG TCACACTCCG GGATTTCAGC AAGTTGAATA AGCAAGATAT CAGATATCTG 
GGGAAAATAT TCAGCTCTGC CACAAGCCTC AGGCTGCAAA TAAAGAGATG TGCTGGTGTG 
GCTGGAAGCC TCAGTTTGGT CCTCAGCACC TGTAAGAACA TTTATTCTCT CATGGTGGAA 
GCCAGTCCCC TCACCATAGA AGATGAGAGG CACATCACAT CTGTAACAAA CCTGAAAACC 
TTGAGTATTC ATGACCTACA GAATCAACGG CTGCCGGGTG GTCTGACTGA CAGCTTGGGT 
AACTTGAAGA ACCTTACAAA GCTCATAATG GATAACATAA AGATGAATGA AGAAGATGCT 
ATAAAACTAG CTGAAGGCCT GAAAAACCTG AAGAAGATGT GTTTATTTCA TTTGACCCAC 
TTGTCTGACA TTGGAGAGGG AATGGATTAC ATAGTCAAGT CTCTGTCAAG TGAACCCTGT 
GACCTTGAAG AAATTCAATT AGTCTCCTGC TGCTTGTCTG CAAATGCAGT GAAAATCCTA 
GCTCAGAATC TTCACAATTT GGTCAAACTG AGCATTCTTG ATTTATCAGA AAATTACCTG 
GAAAAAGATG GAAATGAAGC TCTTCATGAA CTGATCGACA GGATGAACGT GCTAGAACAG 
CTCACCGCAC TGATGCTGCC CTGGGGCTGT GACGTGCAAG GCAGCCTGAG CAGCCTGTTG 
AAACATTTGG AGGAGGTCCC ACAACTCGTC AAGCTTGGGT TGAAAAACTG GAGACTCACA 
GATACAGAGA TTAGAATTTT AGGTGCATTT TTTGGAAAGA ACCCTCTGAA AAACTTCCAG 
CAGTTGAATT TGGCGGGAAA TCGTGTGAGC AGTGATGGAT GGCTTGCCTT CATGGGTGTA 
TTTGAGAATC TTAAGCAATT AGTGTTTTTT GACTTTAGTA CTAAAGAATT TCTACCTGAT 
CCAGCATTAG TCAGAAAACT TAGCCAAGTG TTATCCAAGT TAACTTTTCT GCAAGAAGCT 
AGGCTTGTTG GGTGGCAATT TGATGATGAT GATCTCAGTG TTATTACAGG TGCTTTTAAA 
CTAGTAACTG CTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16706
Clone ID Related Accession (Same CDS sequence) NM_021209.4 , NM_001199139.1 , NM_001199138.1 , NM_001199138.2
Accession Version NM_001199138.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3075bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-30
Organism Homo sapiens(human)
Product NLR family CARD domain-containing protein 4 isoform a
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL121653.2, AK095467.1 and BC031555.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_001199138.1. Transcript Variant: This variant (2) has an alternate 5' UTR exon and encodes the same protein, as compared to variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC031555.1, AK095467.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000402280.6/ ENSP00000385428.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
ATGAATTTCA TAAAGGACAA TAGCCGAGCC CTTATTCAAA GAATGGGAAT GACTGTTATA 
AAGCAAATCA CAGATGACCT ATTTGTATGG AATGTTCTGA ATCGCGAAGA AGTAAACATC 
ATTTGCTGCG AGAAGGTGGA GCAGGATGCT GCTAGAGGGA TCATTCACAT GATTTTGAAA 
AAGGGTTCAG AGTCCTGTAA CCTCTTTCTT AAATCCCTTA AGGAGTGGAA CTATCCTCTA 
TTTCAGGACT TGAATGGACA AAGTCTTTTT CATCAGACAT CAGAAGGAGA CTTGGACGAT 
TTGGCTCAGG ATTTAAAGGA CTTGTACCAT ACCCCATCTT TTCTGAACTT TTATCCCCTT 
GGTGAAGATA TTGACATTAT TTTTAACTTG AAAAGCACCT TCACAGAACC TGTCCTGTGG 
AGGAAGGACC AACACCATCA CCGCGTGGAG CAGCTGACCC TGAATGGCCT CCTGCAGGCT 
CTTCAGAGCC CCTGCATCAT TGAAGGGGAA TCTGGCAAAG GCAAGTCCAC TCTGCTGCAG 
CGAATTGCCA TGCTCTGGGG CTCCGGAAAG TGCAAGGCTC TGACCAAGTT CAAATTCGTC 
TTCTTCCTCC GTCTCAGCAG GGCCCAGGGT GGACTTTTTG AAACCCTCTG TGATCAACTC 
CTGGATATAC CTGGCACAAT CAGGAAGCAG ACATTCATGG CCATGCTGCT GAAGCTGCGG 
CAGAGGGTTC TTTTCCTTCT TGATGGCTAC AATGAATTCA AGCCCCAGAA CTGCCCAGAA 
ATCGAAGCCC TGATAAAGGA AAACCACCGC TTCAAGAACA TGGTCATCGT CACCACTACC 
ACTGAGTGCC TGAGGCACAT ACGGCAGTTT GGTGCCCTGA CTGCTGAGGT GGGGGATATG 
ACAGAAGACA GCGCCCAGGC TCTCATCCGA GAAGTGCTGA TCAAGGAGCT TGCTGAAGGC 
TTGTTGCTCC AAATTCAGAA ATCCAGGTGC TTGAGGAATC TCATGAAGAC CCCTCTCTTT 
GTGGTCATCA CTTGTGCAAT CCAGATGGGT GAAAGTGAGT TCCACTCTCA CACACAAACA 
ACGCTGTTCC ATACCTTCTA TGATCTGTTG ATACAGAAAA ACAAACACAA ACATAAAGGT 
GTGGCTGCAA GTGACTTCAT TCGGAGCCTG GACCACTGTG GAGACCTAGC TCTGGAGGGT 
GTGTTCTCCC ACAAGTTTGA TTTCGAACTG CAGGATGTGT CCAGCGTGAA TGAGGATGTC 
CTGCTGACAA CTGGGCTCCT CTGTAAATAT ACAGCTCAAA GGTTCAAGCC AAAGTATAAA 
TTCTTTCACA AGTCATTCCA GGAGTACACA GCAGGACGAA GACTCAGCAG TTTATTGACG 
TCTCATGAGC CAGAGGAGGT GACCAAGGGG AATGGTTACT TGCAGAAAAT GGTTTCCATT 
TCGGACATTA CATCCACTTA TAGCAGCCTG CTCCGGTACA CCTGTGGGTC ATCTGTGGAA 
GCCACCAGGG CTGTTATGAA GCACCTCGCA GCAGTGTATC AACACGGCTG CCTTCTCGGA 
CTTTCCATCG CCAAGAGGCC TCTCTGGAGA CAGGAATCTT TGCAAAGTGT GAAAAACACC 
ACTGAGCAAG AAATTCTGAA AGCCATAAAC ATCAATTCCT TTGTAGAGTG TGGCATCCAT 
TTATATCAAG AGAGTACATC CAAATCAGCC CTGAGCCAAG AATTTGAAGC TTTCTTTCAA 
GGTAAAAGCT TATATATCAA CTCAGGGAAC ATCCCCGATT ACTTATTTGA CTTCTTTGAA 
CATTTGCCCA ATTGTGCAAG TGCCCTGGAC TTCATTAAAC TGGACTTTTA TGGGGGAGCT 
ATGGCTTCAT GGGAAAAGGC TGCAGAAGAC ACAGGTGGAA TCCACATGGA AGAGGCCCCA 
GAAACCTACA TTCCCAGCAG GGCTGTATCT TTGTTCTTCA ACTGGAAGCA GGAATTCAGG 
ACTCTGGAGG TCACACTCCG GGATTTCAGC AAGTTGAATA AGCAAGATAT CAGATATCTG 
GGGAAAATAT TCAGCTCTGC CACAAGCCTC AGGCTGCAAA TAAAGAGATG TGCTGGTGTG 
GCTGGAAGCC TCAGTTTGGT CCTCAGCACC TGTAAGAACA TTTATTCTCT CATGGTGGAA 
GCCAGTCCCC TCACCATAGA AGATGAGAGG CACATCACAT CTGTAACAAA CCTGAAAACC 
TTGAGTATTC ATGACCTACA GAATCAACGG CTGCCGGGTG GTCTGACTGA CAGCTTGGGT 
AACTTGAAGA ACCTTACAAA GCTCATAATG GATAACATAA AGATGAATGA AGAAGATGCT 
ATAAAACTAG CTGAAGGCCT GAAAAACCTG AAGAAGATGT GTTTATTTCA TTTGACCCAC 
TTGTCTGACA TTGGAGAGGG AATGGATTAC ATAGTCAAGT CTCTGTCAAG TGAACCCTGT 
GACCTTGAAG AAATTCAATT AGTCTCCTGC TGCTTGTCTG CAAATGCAGT GAAAATCCTA 
GCTCAGAATC TTCACAATTT GGTCAAACTG AGCATTCTTG ATTTATCAGA AAATTACCTG 
GAAAAAGATG GAAATGAAGC TCTTCATGAA CTGATCGACA GGATGAACGT GCTAGAACAG 
CTCACCGCAC TGATGCTGCC CTGGGGCTGT GACGTGCAAG GCAGCCTGAG CAGCCTGTTG 
AAACATTTGG AGGAGGTCCC ACAACTCGTC AAGCTTGGGT TGAAAAACTG GAGACTCACA 
GATACAGAGA TTAGAATTTT AGGTGCATTT TTTGGAAAGA ACCCTCTGAA AAACTTCCAG 
CAGTTGAATT TGGCGGGAAA TCGTGTGAGC AGTGATGGAT GGCTTGCCTT CATGGGTGTA 
TTTGAGAATC TTAAGCAATT AGTGTTTTTT GACTTTAGTA CTAAAGAATT TCTACCTGAT 
CCAGCATTAG TCAGAAAACT TAGCCAAGTG TTATCCAAGT TAACTTTTCT GCAAGAAGCT 
AGGCTTGTTG GGTGGCAATT TGATGATGAT GATCTCAGTG TTATTACAGG TGCTTTTAAA 
CTAGTAACTG CTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001186067.1
CDS268..3342
Translation

Target ORF information:

RefSeq Version NM_001199138.2
Organism Homo sapiens(human)
Definition Homo sapiens NLR family CARD domain containing 4 (NLRC4), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001199138.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
ATGAATTTCA TAAAGGACAA TAGCCGAGCC CTTATTCAAA GAATGGGAAT GACTGTTATA 
AAGCAAATCA CAGATGACCT ATTTGTATGG AATGTTCTGA ATCGCGAAGA AGTAAACATC 
ATTTGCTGCG AGAAGGTGGA GCAGGATGCT GCTAGAGGGA TCATTCACAT GATTTTGAAA 
AAGGGTTCAG AGTCCTGTAA CCTCTTTCTT AAATCCCTTA AGGAGTGGAA CTATCCTCTA 
TTTCAGGACT TGAATGGACA AAGTCTTTTT CATCAGACAT CAGAAGGAGA CTTGGACGAT 
TTGGCTCAGG ATTTAAAGGA CTTGTACCAT ACCCCATCTT TTCTGAACTT TTATCCCCTT 
GGTGAAGATA TTGACATTAT TTTTAACTTG AAAAGCACCT TCACAGAACC TGTCCTGTGG 
AGGAAGGACC AACACCATCA CCGCGTGGAG CAGCTGACCC TGAATGGCCT CCTGCAGGCT 
CTTCAGAGCC CCTGCATCAT TGAAGGGGAA TCTGGCAAAG GCAAGTCCAC TCTGCTGCAG 
CGAATTGCCA TGCTCTGGGG CTCCGGAAAG TGCAAGGCTC TGACCAAGTT CAAATTCGTC 
TTCTTCCTCC GTCTCAGCAG GGCCCAGGGT GGACTTTTTG AAACCCTCTG TGATCAACTC 
CTGGATATAC CTGGCACAAT CAGGAAGCAG ACATTCATGG CCATGCTGCT GAAGCTGCGG 
CAGAGGGTTC TTTTCCTTCT TGATGGCTAC AATGAATTCA AGCCCCAGAA CTGCCCAGAA 
ATCGAAGCCC TGATAAAGGA AAACCACCGC TTCAAGAACA TGGTCATCGT CACCACTACC 
ACTGAGTGCC TGAGGCACAT ACGGCAGTTT GGTGCCCTGA CTGCTGAGGT GGGGGATATG 
ACAGAAGACA GCGCCCAGGC TCTCATCCGA GAAGTGCTGA TCAAGGAGCT TGCTGAAGGC 
TTGTTGCTCC AAATTCAGAA ATCCAGGTGC TTGAGGAATC TCATGAAGAC CCCTCTCTTT 
GTGGTCATCA CTTGTGCAAT CCAGATGGGT GAAAGTGAGT TCCACTCTCA CACACAAACA 
ACGCTGTTCC ATACCTTCTA TGATCTGTTG ATACAGAAAA ACAAACACAA ACATAAAGGT 
GTGGCTGCAA GTGACTTCAT TCGGAGCCTG GACCACTGTG GAGACCTAGC TCTGGAGGGT 
GTGTTCTCCC ACAAGTTTGA TTTCGAACTG CAGGATGTGT CCAGCGTGAA TGAGGATGTC 
CTGCTGACAA CTGGGCTCCT CTGTAAATAT ACAGCTCAAA GGTTCAAGCC AAAGTATAAA 
TTCTTTCACA AGTCATTCCA GGAGTACACA GCAGGACGAA GACTCAGCAG TTTATTGACG 
TCTCATGAGC CAGAGGAGGT GACCAAGGGG AATGGTTACT TGCAGAAAAT GGTTTCCATT 
TCGGACATTA CATCCACTTA TAGCAGCCTG CTCCGGTACA CCTGTGGGTC ATCTGTGGAA 
GCCACCAGGG CTGTTATGAA GCACCTCGCA GCAGTGTATC AACACGGCTG CCTTCTCGGA 
CTTTCCATCG CCAAGAGGCC TCTCTGGAGA CAGGAATCTT TGCAAAGTGT GAAAAACACC 
ACTGAGCAAG AAATTCTGAA AGCCATAAAC ATCAATTCCT TTGTAGAGTG TGGCATCCAT 
TTATATCAAG AGAGTACATC CAAATCAGCC CTGAGCCAAG AATTTGAAGC TTTCTTTCAA 
GGTAAAAGCT TATATATCAA CTCAGGGAAC ATCCCCGATT ACTTATTTGA CTTCTTTGAA 
CATTTGCCCA ATTGTGCAAG TGCCCTGGAC TTCATTAAAC TGGACTTTTA TGGGGGAGCT 
ATGGCTTCAT GGGAAAAGGC TGCAGAAGAC ACAGGTGGAA TCCACATGGA AGAGGCCCCA 
GAAACCTACA TTCCCAGCAG GGCTGTATCT TTGTTCTTCA ACTGGAAGCA GGAATTCAGG 
ACTCTGGAGG TCACACTCCG GGATTTCAGC AAGTTGAATA AGCAAGATAT CAGATATCTG 
GGGAAAATAT TCAGCTCTGC CACAAGCCTC AGGCTGCAAA TAAAGAGATG TGCTGGTGTG 
GCTGGAAGCC TCAGTTTGGT CCTCAGCACC TGTAAGAACA TTTATTCTCT CATGGTGGAA 
GCCAGTCCCC TCACCATAGA AGATGAGAGG CACATCACAT CTGTAACAAA CCTGAAAACC 
TTGAGTATTC ATGACCTACA GAATCAACGG CTGCCGGGTG GTCTGACTGA CAGCTTGGGT 
AACTTGAAGA ACCTTACAAA GCTCATAATG GATAACATAA AGATGAATGA AGAAGATGCT 
ATAAAACTAG CTGAAGGCCT GAAAAACCTG AAGAAGATGT GTTTATTTCA TTTGACCCAC 
TTGTCTGACA TTGGAGAGGG AATGGATTAC ATAGTCAAGT CTCTGTCAAG TGAACCCTGT 
GACCTTGAAG AAATTCAATT AGTCTCCTGC TGCTTGTCTG CAAATGCAGT GAAAATCCTA 
GCTCAGAATC TTCACAATTT GGTCAAACTG AGCATTCTTG ATTTATCAGA AAATTACCTG 
GAAAAAGATG GAAATGAAGC TCTTCATGAA CTGATCGACA GGATGAACGT GCTAGAACAG 
CTCACCGCAC TGATGCTGCC CTGGGGCTGT GACGTGCAAG GCAGCCTGAG CAGCCTGTTG 
AAACATTTGG AGGAGGTCCC ACAACTCGTC AAGCTTGGGT TGAAAAACTG GAGACTCACA 
GATACAGAGA TTAGAATTTT AGGTGCATTT TTTGGAAAGA ACCCTCTGAA AAACTTCCAG 
CAGTTGAATT TGGCGGGAAA TCGTGTGAGC AGTGATGGAT GGCTTGCCTT CATGGGTGTA 
TTTGAGAATC TTAAGCAATT AGTGTTTTTT GACTTTAGTA CTAAAGAATT TCTACCTGAT 
CCAGCATTAG TCAGAAAACT TAGCCAAGTG TTATCCAAGT TAACTTTTCT GCAAGAAGCT 
AGGCTTGTTG GGTGGCAATT TGATGATGAT GATCTCAGTG TTATTACAGG TGCTTTTAAA 
CTAGTAACTG CTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed