ORF cDNA Clones and Custom Clones » Species Summary » Rattus norvegicus » Htr1f cDNA ORF clone

Htr1f cDNA ORF clone, Rattus norvegicus(Norway rat)

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Summary

Gene Symbol	Htr1f
Entrez Gene ID	60448
Full Name	5-hydroxytryptamine receptor 1F
Synonyms	Htr1eb
General protein information	Preferred Names 5-hydroxytryptamine receptor 1F Names 5-hydroxytryptamine receptor 1F 5-HT-1F 5-HT1F 5-hydroxytryptamine (serotonin) receptor 1F, G protein-coupled serotonin receptor 1F serotonin receptor gene (similar to Mm Htr1eb)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 11 Map Location: 11p12
Summary	G-protein coupled receptor for serotonin [RGD, Feb 2006].

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA	Protein	Name
NM_021857.3	NP_068629.2	5-hydroxytryptamine receptor 1F

Homologies

Xenopus tropicalis (tropical clawed frog)	htr1f	XP_002931817.1
Canis lupus familiaris (dog)	HTR1F	XP_005638799.1
Mus musculus (house mouse)	Htr1f	NP_032336.1
Gallus gallus (chicken)	HTR1F	XP_004938391.1
Homo sapiens (human)	HTR1F	NP_000857.1
Danio rerio (zebrafish)	htr1fa	XP_001344430.1
	HTR1F	XP_001081998.1
Bos taurus (cattle)	HTR1F	NP_001193534.1
Anopheles gambiae (African malaria mosquito)	GPR5HT1B	XP_317820.4
Pan troglodytes (chimpanzee)	HTR1F	NP_001182198.1
Rattus norvegicus (Norway rat)	Htr1f	NP_068629.2

Bibliography

Related articles in PubMed

Polysynaptic excitatory postsynaptic potentials that trigger spasms after spinal cord injury in rats are inhibited by 5-HT1B and 5-HT1F receptors.
Murray KC, Stephens MJ, Rank M, D'Amico J, Gorassini MA, Bennett DJ
Journal of neurophysiology106(2)925-43(2011 Aug)

Toward selective drug development for the human 5-hydroxytryptamine 1E receptor: a comparison of 5-hydroxytryptamine 1E and 1F receptor structure-affinity relationships.
Klein MT, Dukat M, Glennon RA, Teitler M
The Journal of pharmacology and experimental therapeutics337(3)860-7(2011 Jun)

Distribution of 5-HT(1B), 5-HT(1D) and 5-HT(1F) receptor expression in rat trigeminal and dorsal root ganglia neurons: relevance to the selective anti-migraine effect of triptans.
Classey JD, Bartsch T, Goadsby PJ
Brain research136176-85(2010 Nov)

Colocalization of 5-HT1F receptor and calcitonin gene-related peptide in rat vestibular nuclei.
Ahn SK, Khalmuratova R, Jeon SY, Kim JP, Park JJ, Hur DG, Balaban CD
Neuroscience letters465(2)151-6(2009 Nov)

Colocalization of 5-HT1F receptor and glutamate in neurons of the vestibular nuclei in rats.
Ahn SK, Khalmuratova R, Jeon SY, Kim JP, Park JJ, Hur DG, Kim DW, Balaban CD
Neuroreport20(2)111-5(2009 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Polysynaptic excitatory postsynaptic potentials that trigger spasms after spinal cord injury are regulated by 5-HT1F and 5-HT1B receptors.
Title: Polysynaptic excitatory postsynaptic potentials that trigger spasms after spinal cord injury in rats are inhibited by 5-HT1B and 5-HT1F receptors.

5-HT(1B), 5-HT(1D)and 5-HT(1F) receptor expression are equally represented in trigeminal ganglia and the dorsal root ganglia.
Title: Distribution of 5-HT(1B), 5-HT(1D) and 5-HT(1F) receptor expression in rat trigeminal and dorsal root ganglia neurons: relevance to the selective anti-migraine effect of triptans.

This study demonstrates the colocalization of 5-HT(1F) receptor and CGRP in the rat vestibular nuclei, indicates that 5-HT(1F) receptor agonists may ameliorate migrainous vertigo by attenuating elevated levels of CGRP release from vestibular nuclei.
Title: Colocalization of 5-HT1F receptor and calcitonin gene-related peptide in rat vestibular nuclei.

colocalization of 5-HT1F receptor and glutamate in the vestibular nuclei wsas observed; this suggests that 5-HT1F receptor might modulate glutamate release from the vestibular nuclei
Title: Colocalization of 5-HT1F receptor and glutamate in neurons of the vestibular nuclei in rats.

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The following Htr1f gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Htr1f cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa42856	NM_021857.3 Latest version!	Rattus norvegicus 5-hydroxytryptamine receptor 1F (Htr1f), mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	5-7	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Htr1f ( NM_021857.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_068629.2 Rattus norvegicus 5-hydroxytryptamine receptor 1F (Htr1f), mRNA.

Price & Availability↑

CloneID	ORa42856
Clone ID Related Accession (Same CDS sequence)	NM_021857.3
Accession Version	NM_021857.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1101bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	5-hydroxytryptamine receptor 1F
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07033025.1. On Jul 7, 2012 this sequence version replaced NM_021857.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

ATGGATTTTC TAAACTCATC AGACCAAAAT TTAACCTCAG AGGAACTGTT AAACCGAATG 
CCATCCAAAA TTCTGGTATC CCTCACTCTG TCTGGCCTGG CCTTGATGAC AACCACCATC 
AACTCCCTCG TGATCACTGC AATCATTGTG ACTCGGAAGC TGCACCACCC AGCCAACTAT 
TTAATCTGTT CCTTGGCAGT TACAGACTTT CTTGTTGCTG TCCTGGTTAT GCCCTTTAGC 
ATCGTGTACA TTGTGAGCGA GAGTTGGATT ATGGGACAAG GACTCTGTGA CCTTTGGCTG 
AGTGTTGACA TCATCTGCTG TAGCTGCTCC ATCTTGCACC TGTCGGCTAT AGCGTTGGAT 
AGGTACCGAG CAATCACAGA CGCAGTCGAG TATGCCAGGA AGAGGACTCC CAGGCATGCT 
GGCATCACGA TTACAACAGT GTGGGTTATA TCTGTGTTCA TCTCCATGCC TCCTCTCTTC 
TGGAGGCACC AAGGAAATAG CCGTGATGAT CAGTGTATCA TCAAACATGA CCATATTGTT 
TCCACAATTT ACTCCACGTT TGGAGCCTTC TACATCCCAC TTGTGTTGAT ATTGATCCTC 
TACTACAAAA TATACAGAGC AGCAAGGACA CTATACCACA AGAGACAAGC AAGTCGGATG 
ATAAAGGAGG AACTGAATGG CCAAGTCCTT TTGGAGAGTG GTGAGAAGAG CATTAAACTG 
GTCTCCACAT CCTACATGTT AGAAAAATCC TTATCTGATC CATCAACAGA CTTTGATAGA 
ATTCACAGCA CAGTGAAAAG TCCCAGATCT GAGCTGAAGC ACGAGAAATC TTGGAGAAGA 
CAGAAAATCT CAGGCACTCG AGAACGCAAA GCAGCCACTA CCCTGGGATT GATCTTGGGC 
GCATTTGTAA TATGTTGGTT GCCCTTTTTT GTAAAGGAAT TGGTTGTTAA TATCTGTGAA 
AAATGTAAAA TTTCTGAAGA AATGTCAAAT TTTTTGGCAT GGCTTGGTTA CCTGAATTCT 
CTTATAAACC CACTGATTTA TACCATCTTT AATGAAGACT TTAAGAAAGC CTTCCAAAAA 
CTTGTACGAT GTCGAAATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_068629.2
CDS	42..1142
Translation	MDFLNSSDQNLTSEELLNRMPSKILVSLTLSGLALMTTTINSLVITAIIVTRKLHHPANYLICSLAVTDFLVAVLVMPFSIVYIVSESWIMGQGLCDLWLSVDIICCSCSILHLSAIALDRYRAITDAVEYARKRTPRHAGITITTVWVISVFISMPPLFWRHQGNSRDDQCIIKHDHIVSTIYSTFGAFYIPLVLILILYYKIYRAARTLYHKRQASRMIKEELNGQVLLESGEKSIKLVSTSYMLEKSLSDPSTDFDRIHSTVKSPRSELKHEKSWRRQKISGTRERKAATTLGLILGAFVICWLPFFVKELVVNICEKCKISEEMSNFLAWLGYLNSLINPLIYTIFNEDFKKAFQKLVRCRN

Target ORF information:

RefSeq Version	NM_021857.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus 5-hydroxytryptamine receptor 1F (Htr1f), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021857.3

ORF Insert Sequence:

ATGGATTTTC TAAACTCATC AGACCAAAAT TTAACCTCAG AGGAACTGTT AAACCGAATG 
CCATCCAAAA TTCTGGTATC CCTCACTCTG TCTGGCCTGG CCTTGATGAC AACCACCATC 
AACTCCCTCG TGATCACTGC AATCATTGTG ACTCGGAAGC TGCACCACCC AGCCAACTAT 
TTAATCTGTT CCTTGGCAGT TACAGACTTT CTTGTTGCTG TCCTGGTTAT GCCCTTTAGC 
ATCGTGTACA TTGTGAGCGA GAGTTGGATT ATGGGACAAG GACTCTGTGA CCTTTGGCTG 
AGTGTTGACA TCATCTGCTG TAGCTGCTCC ATCTTGCACC TGTCGGCTAT AGCGTTGGAT 
AGGTACCGAG CAATCACAGA CGCAGTCGAG TATGCCAGGA AGAGGACTCC CAGGCATGCT 
GGCATCACGA TTACAACAGT GTGGGTTATA TCTGTGTTCA TCTCCATGCC TCCTCTCTTC 
TGGAGGCACC AAGGAAATAG CCGTGATGAT CAGTGTATCA TCAAACATGA CCATATTGTT 
TCCACAATTT ACTCCACGTT TGGAGCCTTC TACATCCCAC TTGTGTTGAT ATTGATCCTC 
TACTACAAAA TATACAGAGC AGCAAGGACA CTATACCACA AGAGACAAGC AAGTCGGATG 
ATAAAGGAGG AACTGAATGG CCAAGTCCTT TTGGAGAGTG GTGAGAAGAG CATTAAACTG 
GTCTCCACAT CCTACATGTT AGAAAAATCC TTATCTGATC CATCAACAGA CTTTGATAGA 
ATTCACAGCA CAGTGAAAAG TCCCAGATCT GAGCTGAAGC ACGAGAAATC TTGGAGAAGA 
CAGAAAATCT CAGGCACTCG AGAACGCAAA GCAGCCACTA CCCTGGGATT GATCTTGGGC 
GCATTTGTAA TATGTTGGTT GCCCTTTTTT GTAAAGGAAT TGGTTGTTAA TATCTGTGAA 
AAATGTAAAA TTTCTGAAGA AATGTCAAAT TTTTTGGCAT GGCTTGGTTA CCTGAATTCT 
CTTATAAACC CACTGATTTA TACCATCTTT AATGAAGACT TTAAGAAAGC CTTCCAAAAA 
CTTGTACGAT GTCGAAATTA G