Trmt6 cDNA ORF clone, Mus musculus(house mouse)

  • Gene

  • Clones

  • gRNAs

The following Trmt6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Trmt6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu30805 NM_001356443.1
Latest version!
Mus musculus tRNA methyltransferase 6 (Trmt6), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $139.30
$199.00
OMu30806 NM_001356444.1
Latest version!
Mus musculus tRNA methyltransferase 6 (Trmt6), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $139.30
$199.00
OMu30805 XM_006500051.2
Latest version!
Mus musculus tRNA methyltransferase 6 (Trmt6), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $139.30
$199.00
OMu30806 XM_006500052.2
Latest version!
Mus musculus tRNA methyltransferase 6 (Trmt6), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $139.30
$199.00
OMu14869 NM_175113.3
Latest version!
Mus musculus tRNA methyltransferase 6 (Trmt6), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $223.30
$319.00
OMu83644 NM_001356442.1
Latest version!
Mus musculus tRNA methyltransferase 6 (Trmt6), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OMu30806 XM_030251972.1
Latest version!
Mus musculus tRNA methyltransferase 6 (Trmt6), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu30805
    Clone ID Related Accession (Same CDS sequence) XM_006500051.2 , NM_001356443.1
    Accession Version NM_001356443.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 981bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-21
    Organism Mus musculus(house mouse)
    Product tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL929562.12. On Oct 4, 2017 this sequence version replaced XM_006500051.2. Transcript Variant: This variant (3) uses an alternate splice junction in the 5' end compared to variant 1. The resulting isoform (3) is shorter at the N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660815.78273.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164137 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGTATTATG CAAGAGAACC TGGAAAAATT AACCACATGA GATATGATAC ACTAGCCCAG 
    ATGTTGACGT TGGGAAATAT CCGTGCCGGC AATAAAATGA TTGTCATGGA AACGTGCTCG
    GGCTTGGTGC TAGGTGCCAT GATGGAACGA ATGGGAGGCT TTGGCTCCAT TATTCAGCTG
    TATCCTGGAG ATGGACCCGT TCGGGCGGCA ACAGCATGTT TTGGATTTCC CAAATCTTTC
    CTCAGTGGTC TTTACGAGTT CCCTCTCAAC AAAGTAAACA GTCTCCTCAA TGGGACATTT
    TCTGCTGAGA TGCTGTCCTC AGAGCCTAAA GACAGCACTC CAGTTGAAGA AAGTAACGGT
    GAGCTTGAGG AGAAAGAGAT TGCTGAACAA GCGGATGAAG ACAACATTGT GGATGCCGCA
    GAAAACAACT CAGGAGAACA GAGACCAATG GAGATTGTTC CTGGGGACCC AGAGAATAAG
    GAGCCCAAAG AAAAAAGAAG CAAAAGAGAT TATATTCAGG AAAAGCAAAG GAGACAAGAA
    GAGCAGAGGA AAAGACATCT GGAGGCTGCT GCTCTGCTGG GAGAAAGAAA TGCAGATGGT
    TTGATTGTGG CCAGTCGTTT CCACCCCACG CCCCTGCTGC TGTCTTTGCT GGACTTTGTA
    GCCCCGTCAA GGCCGTTTGT GGTCTACTGT CAGTATAAAG AGCCTTTGTT GGAATGCTAC
    ACAAAACTTC GGGAGAGGGG AGGAGTCATT AACCTGAGGT TGTCTGAAAC CTGGCTCAGA
    AATTACCAGG TTCTGCCAGA TCGGAGTCAT CCCAAATTAT TGATGAGTGG CGGTGGAGGG
    TACCTTCTGT CAGGGTTCAC TGTTGTCTCA GACAGCCTTC GGGCAGACCC CAGCCTCAAG
    TCCTGCACAG GCGCTTTAGA CCCACACAAG GCTGAGGAGC CAGCAGCTAA AAAACAGAAA
    TGCATGGAAT CTGCCTCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001343372.1
    CDS807..1787
    Translation

    Target ORF information:

    RefSeq Version NM_001356443.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus tRNA methyltransferase 6 (Trmt6), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001356443.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGTATTATG CAAGAGAACC TGGAAAAATT AACCACATGA GATATGATAC ACTAGCCCAG 
    ATGTTGACGT TGGGAAATAT CCGTGCCGGC AATAAAATGA TTGTCATGGA AACGTGCTCG
    GGCTTGGTGC TAGGTGCCAT GATGGAACGA ATGGGAGGCT TTGGCTCCAT TATTCAGCTG
    TATCCTGGAG ATGGACCCGT TCGGGCGGCA ACAGCATGTT TTGGATTTCC CAAATCTTTC
    CTCAGTGGTC TTTACGAGTT CCCTCTCAAC AAAGTAAACA GTCTCCTCAA TGGGACATTT
    TCTGCTGAGA TGCTGTCCTC AGAGCCTAAA GACAGCACTC CAGTTGAAGA AAGTAACGGT
    GAGCTTGAGG AGAAAGAGAT TGCTGAACAA GCGGATGAAG ACAACATTGT GGATGCCGCA
    GAAAACAACT CAGGAGAACA GAGACCAATG GAGATTGTTC CTGGGGACCC AGAGAATAAG
    GAGCCCAAAG AAAAAAGAAG CAAAAGAGAT TATATTCAGG AAAAGCAAAG GAGACAAGAA
    GAGCAGAGGA AAAGACATCT GGAGGCTGCT GCTCTGCTGG GAGAAAGAAA TGCAGATGGT
    TTGATTGTGG CCAGTCGTTT CCACCCCACG CCCCTGCTGC TGTCTTTGCT GGACTTTGTA
    GCCCCGTCAA GGCCGTTTGT GGTCTACTGT CAGTATAAAG AGCCTTTGTT GGAATGCTAC
    ACAAAACTTC GGGAGAGGGG AGGAGTCATT AACCTGAGGT TGTCTGAAAC CTGGCTCAGA
    AATTACCAGG TTCTGCCAGA TCGGAGTCAT CCCAAATTAT TGATGAGTGG CGGTGGAGGG
    TACCTTCTGT CAGGGTTCAC TGTTGTCTCA GACAGCCTTC GGGCAGACCC CAGCCTCAAG
    TCCTGCACAG GCGCTTTAGA CCCACACAAG GCTGAGGAGC CAGCAGCTAA AAAACAGAAA
    TGCATGGAAT CTGCCTCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu30806
    Clone ID Related Accession (Same CDS sequence) NM_001356444.1 , XM_006500052.2 , XM_030251972.1
    Accession Version NM_001356444.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 945bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-22
    Organism Mus musculus(house mouse)
    Product tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 isoform 4
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL929562.12. On Oct 4, 2017 this sequence version replaced XM_006500052.2. Transcript Variant: This variant (4) uses an alternate splice junction compared to variant 1. The resulting isoform (4) is shorter at the N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK014359.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAGATATG ATACACTAGC CCAGATGTTG ACGTTGGGAA ATATCCGTGC CGGCAATAAA 
    ATGATTGTCA TGGAAACGTG CTCGGGCTTG GTGCTAGGTG CCATGATGGA ACGAATGGGA
    GGCTTTGGCT CCATTATTCA GCTGTATCCT GGAGATGGAC CCGTTCGGGC GGCAACAGCA
    TGTTTTGGAT TTCCCAAATC TTTCCTCAGT GGTCTTTACG AGTTCCCTCT CAACAAAGTA
    AACAGTCTCC TCAATGGGAC ATTTTCTGCT GAGATGCTGT CCTCAGAGCC TAAAGACAGC
    ACTCCAGTTG AAGAAAGTAA CGGTGAGCTT GAGGAGAAAG AGATTGCTGA ACAAGCGGAT
    GAAGACAACA TTGTGGATGC CGCAGAAAAC AACTCAGGAG AACAGAGACC AATGGAGATT
    GTTCCTGGGG ACCCAGAGAA TAAGGAGCCC AAAGAAAAAA GAAGCAAAAG AGATTATATT
    CAGGAAAAGC AAAGGAGACA AGAAGAGCAG AGGAAAAGAC ATCTGGAGGC TGCTGCTCTG
    CTGGGAGAAA GAAATGCAGA TGGTTTGATT GTGGCCAGTC GTTTCCACCC CACGCCCCTG
    CTGCTGTCTT TGCTGGACTT TGTAGCCCCG TCAAGGCCGT TTGTGGTCTA CTGTCAGTAT
    AAAGAGCCTT TGTTGGAATG CTACACAAAA CTTCGGGAGA GGGGAGGAGT CATTAACCTG
    AGGTTGTCTG AAACCTGGCT CAGAAATTAC CAGGTTCTGC CAGATCGGAG TCATCCCAAA
    TTATTGATGA GTGGCGGTGG AGGGTACCTT CTGTCAGGGT TCACTGTTGT CTCAGACAGC
    CTTCGGGCAG ACCCCAGCCT CAAGTCCTGC ACAGGCGCTT TAGACCCACA CAAGGCTGAG
    GAGCCAGCAG CTAAAAAACA GAAATGCATG GAATCTGCCT CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001343373.1
    CDS789..1733
    Translation

    Target ORF information:

    RefSeq Version NM_001356444.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus tRNA methyltransferase 6 (Trmt6), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001356444.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAGATATG ATACACTAGC CCAGATGTTG ACGTTGGGAA ATATCCGTGC CGGCAATAAA 
    ATGATTGTCA TGGAAACGTG CTCGGGCTTG GTGCTAGGTG CCATGATGGA ACGAATGGGA
    GGCTTTGGCT CCATTATTCA GCTGTATCCT GGAGATGGAC CCGTTCGGGC GGCAACAGCA
    TGTTTTGGAT TTCCCAAATC TTTCCTCAGT GGTCTTTACG AGTTCCCTCT CAACAAAGTA
    AACAGTCTCC TCAATGGGAC ATTTTCTGCT GAGATGCTGT CCTCAGAGCC TAAAGACAGC
    ACTCCAGTTG AAGAAAGTAA CGGTGAGCTT GAGGAGAAAG AGATTGCTGA ACAAGCGGAT
    GAAGACAACA TTGTGGATGC CGCAGAAAAC AACTCAGGAG AACAGAGACC AATGGAGATT
    GTTCCTGGGG ACCCAGAGAA TAAGGAGCCC AAAGAAAAAA GAAGCAAAAG AGATTATATT
    CAGGAAAAGC AAAGGAGACA AGAAGAGCAG AGGAAAAGAC ATCTGGAGGC TGCTGCTCTG
    CTGGGAGAAA GAAATGCAGA TGGTTTGATT GTGGCCAGTC GTTTCCACCC CACGCCCCTG
    CTGCTGTCTT TGCTGGACTT TGTAGCCCCG TCAAGGCCGT TTGTGGTCTA CTGTCAGTAT
    AAAGAGCCTT TGTTGGAATG CTACACAAAA CTTCGGGAGA GGGGAGGAGT CATTAACCTG
    AGGTTGTCTG AAACCTGGCT CAGAAATTAC CAGGTTCTGC CAGATCGGAG TCATCCCAAA
    TTATTGATGA GTGGCGGTGG AGGGTACCTT CTGTCAGGGT TCACTGTTGT CTCAGACAGC
    CTTCGGGCAG ACCCCAGCCT CAAGTCCTGC ACAGGCGCTT TAGACCCACA CAAGGCTGAG
    GAGCCAGCAG CTAAAAAACA GAAATGCATG GAATCTGCCT CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu30805
    Clone ID Related Accession (Same CDS sequence) XM_006500051.2 , NM_001356443.1
    Accession Version XM_006500051.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 981bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(House mouse)
    Product tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006500051.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGTATTATG CAAGAGAACC TGGAAAAATT AACCACATGA GATATGATAC ACTAGCCCAG 
    ATGTTGACGT TGGGAAATAT CCGTGCCGGC AATAAAATGA TTGTCATGGA AACGTGCTCG
    GGCTTGGTGC TAGGTGCCAT GATGGAACGA ATGGGAGGCT TTGGCTCCAT TATTCAGCTG
    TATCCTGGAG ATGGACCCGT TCGGGCGGCA ACAGCATGTT TTGGATTTCC CAAATCTTTC
    CTCAGTGGTC TTTACGAGTT CCCTCTCAAC AAAGTAAACA GTCTCCTCAA TGGGACATTT
    TCTGCTGAGA TGCTGTCCTC AGAGCCTAAA GACAGCACTC CAGTTGAAGA AAGTAACGGT
    GAGCTTGAGG AGAAAGAGAT TGCTGAACAA GCGGATGAAG ACAACATTGT GGATGCCGCA
    GAAAACAACT CAGGAGAACA GAGACCAATG GAGATTGTTC CTGGGGACCC AGAGAATAAG
    GAGCCCAAAG AAAAAAGAAG CAAAAGAGAT TATATTCAGG AAAAGCAAAG GAGACAAGAA
    GAGCAGAGGA AAAGACATCT GGAGGCTGCT GCTCTGCTGG GAGAAAGAAA TGCAGATGGT
    TTGATTGTGG CCAGTCGTTT CCACCCCACG CCCCTGCTGC TGTCTTTGCT GGACTTTGTA
    GCCCCGTCAA GGCCGTTTGT GGTCTACTGT CAGTATAAAG AGCCTTTGTT GGAATGCTAC
    ACAAAACTTC GGGAGAGGGG AGGAGTCATT AACCTGAGGT TGTCTGAAAC CTGGCTCAGA
    AATTACCAGG TTCTGCCAGA TCGGAGTCAT CCCAAATTAT TGATGAGTGG CGGTGGAGGG
    TACCTTCTGT CAGGGTTCAC TGTTGTCTCA GACAGCCTTC GGGCAGACCC CAGCCTCAAG
    TCCTGCACAG GCGCTTTAGA CCCACACAAG GCTGAGGAGC CAGCAGCTAA AAAACAGAAA
    TGCATGGAAT CTGCCTCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006500114.1
    CDS775..1755
    Translation

    Target ORF information:

    RefSeq Version XM_006500051.2
    Organism Mus musculus(House mouse)
    Definition Mus musculus tRNA methyltransferase 6 (Trmt6), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006500051.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGTATTATG CAAGAGAACC TGGAAAAATT AACCACATGA GATATGATAC ACTAGCCCAG 
    ATGTTGACGT TGGGAAATAT CCGTGCCGGC AATAAAATGA TTGTCATGGA AACGTGCTCG
    GGCTTGGTGC TAGGTGCCAT GATGGAACGA ATGGGAGGCT TTGGCTCCAT TATTCAGCTG
    TATCCTGGAG ATGGACCCGT TCGGGCGGCA ACAGCATGTT TTGGATTTCC CAAATCTTTC
    CTCAGTGGTC TTTACGAGTT CCCTCTCAAC AAAGTAAACA GTCTCCTCAA TGGGACATTT
    TCTGCTGAGA TGCTGTCCTC AGAGCCTAAA GACAGCACTC CAGTTGAAGA AAGTAACGGT
    GAGCTTGAGG AGAAAGAGAT TGCTGAACAA GCGGATGAAG ACAACATTGT GGATGCCGCA
    GAAAACAACT CAGGAGAACA GAGACCAATG GAGATTGTTC CTGGGGACCC AGAGAATAAG
    GAGCCCAAAG AAAAAAGAAG CAAAAGAGAT TATATTCAGG AAAAGCAAAG GAGACAAGAA
    GAGCAGAGGA AAAGACATCT GGAGGCTGCT GCTCTGCTGG GAGAAAGAAA TGCAGATGGT
    TTGATTGTGG CCAGTCGTTT CCACCCCACG CCCCTGCTGC TGTCTTTGCT GGACTTTGTA
    GCCCCGTCAA GGCCGTTTGT GGTCTACTGT CAGTATAAAG AGCCTTTGTT GGAATGCTAC
    ACAAAACTTC GGGAGAGGGG AGGAGTCATT AACCTGAGGT TGTCTGAAAC CTGGCTCAGA
    AATTACCAGG TTCTGCCAGA TCGGAGTCAT CCCAAATTAT TGATGAGTGG CGGTGGAGGG
    TACCTTCTGT CAGGGTTCAC TGTTGTCTCA GACAGCCTTC GGGCAGACCC CAGCCTCAAG
    TCCTGCACAG GCGCTTTAGA CCCACACAAG GCTGAGGAGC CAGCAGCTAA AAAACAGAAA
    TGCATGGAAT CTGCCTCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu30806
    Clone ID Related Accession (Same CDS sequence) NM_001356444.1 , XM_006500052.2 , XM_030251972.1
    Accession Version XM_006500052.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 945bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(House mouse)
    Product tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006500052.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAGATATG ATACACTAGC CCAGATGTTG ACGTTGGGAA ATATCCGTGC CGGCAATAAA 
    ATGATTGTCA TGGAAACGTG CTCGGGCTTG GTGCTAGGTG CCATGATGGA ACGAATGGGA
    GGCTTTGGCT CCATTATTCA GCTGTATCCT GGAGATGGAC CCGTTCGGGC GGCAACAGCA
    TGTTTTGGAT TTCCCAAATC TTTCCTCAGT GGTCTTTACG AGTTCCCTCT CAACAAAGTA
    AACAGTCTCC TCAATGGGAC ATTTTCTGCT GAGATGCTGT CCTCAGAGCC TAAAGACAGC
    ACTCCAGTTG AAGAAAGTAA CGGTGAGCTT GAGGAGAAAG AGATTGCTGA ACAAGCGGAT
    GAAGACAACA TTGTGGATGC CGCAGAAAAC AACTCAGGAG AACAGAGACC AATGGAGATT
    GTTCCTGGGG ACCCAGAGAA TAAGGAGCCC AAAGAAAAAA GAAGCAAAAG AGATTATATT
    CAGGAAAAGC AAAGGAGACA AGAAGAGCAG AGGAAAAGAC ATCTGGAGGC TGCTGCTCTG
    CTGGGAGAAA GAAATGCAGA TGGTTTGATT GTGGCCAGTC GTTTCCACCC CACGCCCCTG
    CTGCTGTCTT TGCTGGACTT TGTAGCCCCG TCAAGGCCGT TTGTGGTCTA CTGTCAGTAT
    AAAGAGCCTT TGTTGGAATG CTACACAAAA CTTCGGGAGA GGGGAGGAGT CATTAACCTG
    AGGTTGTCTG AAACCTGGCT CAGAAATTAC CAGGTTCTGC CAGATCGGAG TCATCCCAAA
    TTATTGATGA GTGGCGGTGG AGGGTACCTT CTGTCAGGGT TCACTGTTGT CTCAGACAGC
    CTTCGGGCAG ACCCCAGCCT CAAGTCCTGC ACAGGCGCTT TAGACCCACA CAAGGCTGAG
    GAGCCAGCAG CTAAAAAACA GAAATGCATG GAATCTGCCT CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006500115.1
    CDS790..1734
    Translation

    Target ORF information:

    RefSeq Version XM_006500052.2
    Organism Mus musculus(House mouse)
    Definition Mus musculus tRNA methyltransferase 6 (Trmt6), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006500052.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAGATATG ATACACTAGC CCAGATGTTG ACGTTGGGAA ATATCCGTGC CGGCAATAAA 
    ATGATTGTCA TGGAAACGTG CTCGGGCTTG GTGCTAGGTG CCATGATGGA ACGAATGGGA
    GGCTTTGGCT CCATTATTCA GCTGTATCCT GGAGATGGAC CCGTTCGGGC GGCAACAGCA
    TGTTTTGGAT TTCCCAAATC TTTCCTCAGT GGTCTTTACG AGTTCCCTCT CAACAAAGTA
    AACAGTCTCC TCAATGGGAC ATTTTCTGCT GAGATGCTGT CCTCAGAGCC TAAAGACAGC
    ACTCCAGTTG AAGAAAGTAA CGGTGAGCTT GAGGAGAAAG AGATTGCTGA ACAAGCGGAT
    GAAGACAACA TTGTGGATGC CGCAGAAAAC AACTCAGGAG AACAGAGACC AATGGAGATT
    GTTCCTGGGG ACCCAGAGAA TAAGGAGCCC AAAGAAAAAA GAAGCAAAAG AGATTATATT
    CAGGAAAAGC AAAGGAGACA AGAAGAGCAG AGGAAAAGAC ATCTGGAGGC TGCTGCTCTG
    CTGGGAGAAA GAAATGCAGA TGGTTTGATT GTGGCCAGTC GTTTCCACCC CACGCCCCTG
    CTGCTGTCTT TGCTGGACTT TGTAGCCCCG TCAAGGCCGT TTGTGGTCTA CTGTCAGTAT
    AAAGAGCCTT TGTTGGAATG CTACACAAAA CTTCGGGAGA GGGGAGGAGT CATTAACCTG
    AGGTTGTCTG AAACCTGGCT CAGAAATTAC CAGGTTCTGC CAGATCGGAG TCATCCCAAA
    TTATTGATGA GTGGCGGTGG AGGGTACCTT CTGTCAGGGT TCACTGTTGT CTCAGACAGC
    CTTCGGGCAG ACCCCAGCCT CAAGTCCTGC ACAGGCGCTT TAGACCCACA CAAGGCTGAG
    GAGCCAGCAG CTAAAAAACA GAAATGCATG GAATCTGCCT CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu14869
    Clone ID Related Accession (Same CDS sequence) NM_175113.3
    Accession Version NM_175113.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1494bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-19
    Organism Mus musculus(house mouse)
    Product tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK028755.1. On Jun 10, 2007 this sequence version replaced NM_175113.2. Transcript Variant: This variant (1) encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: AK028755.1, SRR1660817.300884.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164137 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGAGGCAT CAGCTGCGGA GCAGCCGAGC TCACCGCCCC CGCCTCTCGG GGACCACTGC 
    ATCCATGACG GTGACTTCGT GGTGCTGAAG CGAGAAGATG TGTTCAAAGC AGTGCAAGTC
    CAGCGGAGAA AAAAAGTAAC TTTTGAAAAG CAGTGGTTCT ACCTGGATAA CGCCATCGGC
    CATAGTTACG GCTCAGCGTT TGACGTGAGC AGTGGAGGCA GCCTTCAGCT CAGGAAGAAG
    CTGGAGGAGC CCGCGTCAGA GACCAAAGAA GCAGGCACTG ATAATCGAAA TATAGTTGAT
    GATGGAAAGT CCCAGAAACT TACTCAAGAT GATATAAAAG CTCTGAAAGA CAAGGGCATT
    AAAGGAGAGG AAATAGTTCA GCAGCTAATT GAAAATAGTA CAACATTCCG TGACAAGACA
    GAATTTGCCC AAGATAAATA TATTAAAAAG AAGAAGAAAA AATATGAAGC CATCGTTACT
    ATCTTGAAGC CATCTACCCG TATTCTTTCA ATTATGTATT ATGCAAGAGA ACCTGGAAAA
    ATTAACCACA TGAGATATGA TACACTAGCC CAGATGTTGA CGTTGGGAAA TATCCGTGCC
    GGCAATAAAA TGATTGTCAT GGAAACGTGC TCGGGCTTGG TGCTAGGTGC CATGATGGAA
    CGAATGGGAG GCTTTGGCTC CATTATTCAG CTGTATCCTG GAGATGGACC CGTTCGGGCG
    GCAACAGCAT GTTTTGGATT TCCCAAATCT TTCCTCAGTG GTCTTTACGA GTTCCCTCTC
    AACAAAGTAA ACAGTCTCCT CAATGGGACA TTTTCTGCTG AGATGCTGTC CTCAGAGCCT
    AAAGACAGCA CTCCAGTTGA AGAAAGTAAC GGTGAGCTTG AGGAGAAAGA GATTGCTGAA
    CAAGCGGATG AAGACAACAT TGTGGATGCC GCAGAAAACA ACTCAGGAGA ACAGAGACCA
    ATGGAGATTG TTCCTGGGGA CCCAGAGAAT AAGGAGCCCA AAGAAAAAAG AAGCAAAAGA
    GATTATATTC AGGAAAAGCA AAGGAGACAA GAAGAGCAGA GGAAAAGACA TCTGGAGGCT
    GCTGCTCTGC TGGGAGAAAG AAATGCAGAT GGTTTGATTG TGGCCAGTCG TTTCCACCCC
    ACGCCCCTGC TGCTGTCTTT GCTGGACTTT GTAGCCCCGT CAAGGCCGTT TGTGGTCTAC
    TGTCAGTATA AAGAGCCTTT GTTGGAATGC TACACAAAAC TTCGGGAGAG GGGAGGAGTC
    ATTAACCTGA GGTTGTCTGA AACCTGGCTC AGAAATTACC AGGTTCTGCC AGATCGGAGT
    CATCCCAAAT TATTGATGAG TGGCGGTGGA GGGTACCTTC TGTCAGGGTT CACTGTTGTC
    TCAGACAGCC TTCGGGCAGA CCCCAGCCTC AAGTCCTGCA CAGGCGCTTT AGACCCACAC
    AAGGCTGAGG AGCCAGCAGC TAAAAAACAG AAATGCATGG AATCTGCCTC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_780322.2
    CDS121..1614
    Translation

    Target ORF information:

    RefSeq Version NM_175113.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus tRNA methyltransferase 6 (Trmt6), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_175113.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGAGGCAT CAGCTGCGGA GCAGCCGAGC TCACCGCCCC CGCCTCTCGG GGACCACTGC 
    ATCCATGACG GTGACTTCGT GGTGCTGAAG CGAGAAGATG TGTTCAAAGC AGTGCAAGTC
    CAGCGGAGAA AAAAAGTAAC TTTTGAAAAG CAGTGGTTCT ACCTGGATAA CGCCATCGGC
    CATAGTTACG GCTCAGCGTT TGACGTGAGC AGTGGAGGCA GCCTTCAGCT CAGGAAGAAG
    CTGGAGGAGC CCGCGTCAGA GACCAAAGAA GCAGGCACTG ATAATCGAAA TATAGTTGAT
    GATGGAAAGT CCCAGAAACT TACTCAAGAT GATATAAAAG CTCTGAAAGA CAAGGGCATT
    AAAGGAGAGG AAATAGTTCA GCAGCTAATT GAAAATAGTA CAACATTCCG TGACAAGACA
    GAATTTGCCC AAGATAAATA TATTAAAAAG AAGAAGAAAA AATATGAAGC CATCGTTACT
    ATCTTGAAGC CATCTACCCG TATTCTTTCA ATTATGTATT ATGCAAGAGA ACCTGGAAAA
    ATTAACCACA TGAGATATGA TACACTAGCC CAGATGTTGA CGTTGGGAAA TATCCGTGCC
    GGCAATAAAA TGATTGTCAT GGAAACGTGC TCGGGCTTGG TGCTAGGTGC CATGATGGAA
    CGAATGGGAG GCTTTGGCTC CATTATTCAG CTGTATCCTG GAGATGGACC CGTTCGGGCG
    GCAACAGCAT GTTTTGGATT TCCCAAATCT TTCCTCAGTG GTCTTTACGA GTTCCCTCTC
    AACAAAGTAA ACAGTCTCCT CAATGGGACA TTTTCTGCTG AGATGCTGTC CTCAGAGCCT
    AAAGACAGCA CTCCAGTTGA AGAAAGTAAC GGTGAGCTTG AGGAGAAAGA GATTGCTGAA
    CAAGCGGATG AAGACAACAT TGTGGATGCC GCAGAAAACA ACTCAGGAGA ACAGAGACCA
    ATGGAGATTG TTCCTGGGGA CCCAGAGAAT AAGGAGCCCA AAGAAAAAAG AAGCAAAAGA
    GATTATATTC AGGAAAAGCA AAGGAGACAA GAAGAGCAGA GGAAAAGACA TCTGGAGGCT
    GCTGCTCTGC TGGGAGAAAG AAATGCAGAT GGTTTGATTG TGGCCAGTCG TTTCCACCCC
    ACGCCCCTGC TGCTGTCTTT GCTGGACTTT GTAGCCCCGT CAAGGCCGTT TGTGGTCTAC
    TGTCAGTATA AAGAGCCTTT GTTGGAATGC TACACAAAAC TTCGGGAGAG GGGAGGAGTC
    ATTAACCTGA GGTTGTCTGA AACCTGGCTC AGAAATTACC AGGTTCTGCC AGATCGGAGT
    CATCCCAAAT TATTGATGAG TGGCGGTGGA GGGTACCTTC TGTCAGGGTT CACTGTTGTC
    TCAGACAGCC TTCGGGCAGA CCCCAGCCTC AAGTCCTGCA CAGGCGCTTT AGACCCACAC
    AAGGCTGAGG AGCCAGCAGC TAAAAAACAG AAATGCATGG AATCTGCCTC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu83644
    Clone ID Related Accession (Same CDS sequence) NM_001356442.1
    Accession Version NM_001356442.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1401bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-22
    Organism Mus musculus(house mouse)
    Product tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL929562.12. Transcript Variant: This variant (2) uses an alternate splice junction in the 5' end compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660815.171295.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164137 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGTGTTCAA AGCAGTGCAA GTCCAGCGGA GAAAGTGAAA AAGTAACTTT TGAAAAGCAG 
    TGGTTCTACC TGGATAACGC CATCGGCCAT AGTTACGGCT CAGCGTTTGA CGTGAGCAGT
    GGAGGCAGCC TTCAGCTCAG GAAGAAGCTG GAGGAGCCCG CGTCAGAGAC CAAAGAAGCA
    GGCACTGATA ATCGAAATAT AGTTGATGAT GGAAAGTCCC AGAAACTTAC TCAAGATGAT
    ATAAAAGCTC TGAAAGACAA GGGCATTAAA GGAGAGGAAA TAGTTCAGCA GCTAATTGAA
    AATAGTACAA CATTCCGTGA CAAGACAGAA TTTGCCCAAG ATAAATATAT TAAAAAGAAG
    AAGAAAAAAT ATGAAGCCAT CGTTACTATC TTGAAGCCAT CTACCCGTAT TCTTTCAATT
    ATGTATTATG CAAGAGAACC TGGAAAAATT AACCACATGA GATATGATAC ACTAGCCCAG
    ATGTTGACGT TGGGAAATAT CCGTGCCGGC AATAAAATGA TTGTCATGGA AACGTGCTCG
    GGCTTGGTGC TAGGTGCCAT GATGGAACGA ATGGGAGGCT TTGGCTCCAT TATTCAGCTG
    TATCCTGGAG ATGGACCCGT TCGGGCGGCA ACAGCATGTT TTGGATTTCC CAAATCTTTC
    CTCAGTGGTC TTTACGAGTT CCCTCTCAAC AAAGTAAACA GTCTCCTCAA TGGGACATTT
    TCTGCTGAGA TGCTGTCCTC AGAGCCTAAA GACAGCACTC CAGTTGAAGA AAGTAACGGT
    GAGCTTGAGG AGAAAGAGAT TGCTGAACAA GCGGATGAAG ACAACATTGT GGATGCCGCA
    GAAAACAACT CAGGAGAACA GAGACCAATG GAGATTGTTC CTGGGGACCC AGAGAATAAG
    GAGCCCAAAG AAAAAAGAAG CAAAAGAGAT TATATTCAGG AAAAGCAAAG GAGACAAGAA
    GAGCAGAGGA AAAGACATCT GGAGGCTGCT GCTCTGCTGG GAGAAAGAAA TGCAGATGGT
    TTGATTGTGG CCAGTCGTTT CCACCCCACG CCCCTGCTGC TGTCTTTGCT GGACTTTGTA
    GCCCCGTCAA GGCCGTTTGT GGTCTACTGT CAGTATAAAG AGCCTTTGTT GGAATGCTAC
    ACAAAACTTC GGGAGAGGGG AGGAGTCATT AACCTGAGGT TGTCTGAAAC CTGGCTCAGA
    AATTACCAGG TTCTGCCAGA TCGGAGTCAT CCCAAATTAT TGATGAGTGG CGGTGGAGGG
    TACCTTCTGT CAGGGTTCAC TGTTGTCTCA GACAGCCTTC GGGCAGACCC CAGCCTCAAG
    TCCTGCACAG GCGCTTTAGA CCCACACAAG GCTGAGGAGC CAGCAGCTAA AAAACAGAAA
    TGCATGGAAT CTGCCTCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001343371.1
    CDS218..1618
    Translation

    Target ORF information:

    RefSeq Version NM_001356442.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus tRNA methyltransferase 6 (Trmt6), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001356442.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGTGTTCAA AGCAGTGCAA GTCCAGCGGA GAAAGTGAAA AAGTAACTTT TGAAAAGCAG 
    TGGTTCTACC TGGATAACGC CATCGGCCAT AGTTACGGCT CAGCGTTTGA CGTGAGCAGT
    GGAGGCAGCC TTCAGCTCAG GAAGAAGCTG GAGGAGCCCG CGTCAGAGAC CAAAGAAGCA
    GGCACTGATA ATCGAAATAT AGTTGATGAT GGAAAGTCCC AGAAACTTAC TCAAGATGAT
    ATAAAAGCTC TGAAAGACAA GGGCATTAAA GGAGAGGAAA TAGTTCAGCA GCTAATTGAA
    AATAGTACAA CATTCCGTGA CAAGACAGAA TTTGCCCAAG ATAAATATAT TAAAAAGAAG
    AAGAAAAAAT ATGAAGCCAT CGTTACTATC TTGAAGCCAT CTACCCGTAT TCTTTCAATT
    ATGTATTATG CAAGAGAACC TGGAAAAATT AACCACATGA GATATGATAC ACTAGCCCAG
    ATGTTGACGT TGGGAAATAT CCGTGCCGGC AATAAAATGA TTGTCATGGA AACGTGCTCG
    GGCTTGGTGC TAGGTGCCAT GATGGAACGA ATGGGAGGCT TTGGCTCCAT TATTCAGCTG
    TATCCTGGAG ATGGACCCGT TCGGGCGGCA ACAGCATGTT TTGGATTTCC CAAATCTTTC
    CTCAGTGGTC TTTACGAGTT CCCTCTCAAC AAAGTAAACA GTCTCCTCAA TGGGACATTT
    TCTGCTGAGA TGCTGTCCTC AGAGCCTAAA GACAGCACTC CAGTTGAAGA AAGTAACGGT
    GAGCTTGAGG AGAAAGAGAT TGCTGAACAA GCGGATGAAG ACAACATTGT GGATGCCGCA
    GAAAACAACT CAGGAGAACA GAGACCAATG GAGATTGTTC CTGGGGACCC AGAGAATAAG
    GAGCCCAAAG AAAAAAGAAG CAAAAGAGAT TATATTCAGG AAAAGCAAAG GAGACAAGAA
    GAGCAGAGGA AAAGACATCT GGAGGCTGCT GCTCTGCTGG GAGAAAGAAA TGCAGATGGT
    TTGATTGTGG CCAGTCGTTT CCACCCCACG CCCCTGCTGC TGTCTTTGCT GGACTTTGTA
    GCCCCGTCAA GGCCGTTTGT GGTCTACTGT CAGTATAAAG AGCCTTTGTT GGAATGCTAC
    ACAAAACTTC GGGAGAGGGG AGGAGTCATT AACCTGAGGT TGTCTGAAAC CTGGCTCAGA
    AATTACCAGG TTCTGCCAGA TCGGAGTCAT CCCAAATTAT TGATGAGTGG CGGTGGAGGG
    TACCTTCTGT CAGGGTTCAC TGTTGTCTCA GACAGCCTTC GGGCAGACCC CAGCCTCAAG
    TCCTGCACAG GCGCTTTAGA CCCACACAAG GCTGAGGAGC CAGCAGCTAA AAAACAGAAA
    TGCATGGAAT CTGCCTCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu30806
    Clone ID Related Accession (Same CDS sequence) NM_001356444.1 , XM_006500052.2 , XM_030251972.1
    Accession Version XM_030251972.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 945bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAGATATG ATACACTAGC CCAGATGTTG ACGTTGGGAA ATATCCGTGC CGGCAATAAA 
    ATGATTGTCA TGGAAACGTG CTCGGGCTTG GTGCTAGGTG CCATGATGGA ACGAATGGGA
    GGCTTTGGCT CCATTATTCA GCTGTATCCT GGAGATGGAC CCGTTCGGGC GGCAACAGCA
    TGTTTTGGAT TTCCCAAATC TTTCCTCAGT GGTCTTTACG AGTTCCCTCT CAACAAAGTA
    AACAGTCTCC TCAATGGGAC ATTTTCTGCT GAGATGCTGT CCTCAGAGCC TAAAGACAGC
    ACTCCAGTTG AAGAAAGTAA CGGTGAGCTT GAGGAGAAAG AGATTGCTGA ACAAGCGGAT
    GAAGACAACA TTGTGGATGC CGCAGAAAAC AACTCAGGAG AACAGAGACC AATGGAGATT
    GTTCCTGGGG ACCCAGAGAA TAAGGAGCCC AAAGAAAAAA GAAGCAAAAG AGATTATATT
    CAGGAAAAGC AAAGGAGACA AGAAGAGCAG AGGAAAAGAC ATCTGGAGGC TGCTGCTCTG
    CTGGGAGAAA GAAATGCAGA TGGTTTGATT GTGGCCAGTC GTTTCCACCC CACGCCCCTG
    CTGCTGTCTT TGCTGGACTT TGTAGCCCCG TCAAGGCCGT TTGTGGTCTA CTGTCAGTAT
    AAAGAGCCTT TGTTGGAATG CTACACAAAA CTTCGGGAGA GGGGAGGAGT CATTAACCTG
    AGGTTGTCTG AAACCTGGCT CAGAAATTAC CAGGTTCTGC CAGATCGGAG TCATCCCAAA
    TTATTGATGA GTGGCGGTGG AGGGTACCTT CTGTCAGGGT TCACTGTTGT CTCAGACAGC
    CTTCGGGCAG ACCCCAGCCT CAAGTCCTGC ACAGGCGCTT TAGACCCACA CAAGGCTGAG
    GAGCCAGCAG CTAAAAAACA GAAATGCATG GAATCTGCCT CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030107832.1
    CDS945..1889
    Translation

    Target ORF information:

    RefSeq Version XM_030251972.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus tRNA methyltransferase 6 (Trmt6), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030251972.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAGATATG ATACACTAGC CCAGATGTTG ACGTTGGGAA ATATCCGTGC CGGCAATAAA 
    ATGATTGTCA TGGAAACGTG CTCGGGCTTG GTGCTAGGTG CCATGATGGA ACGAATGGGA
    GGCTTTGGCT CCATTATTCA GCTGTATCCT GGAGATGGAC CCGTTCGGGC GGCAACAGCA
    TGTTTTGGAT TTCCCAAATC TTTCCTCAGT GGTCTTTACG AGTTCCCTCT CAACAAAGTA
    AACAGTCTCC TCAATGGGAC ATTTTCTGCT GAGATGCTGT CCTCAGAGCC TAAAGACAGC
    ACTCCAGTTG AAGAAAGTAA CGGTGAGCTT GAGGAGAAAG AGATTGCTGA ACAAGCGGAT
    GAAGACAACA TTGTGGATGC CGCAGAAAAC AACTCAGGAG AACAGAGACC AATGGAGATT
    GTTCCTGGGG ACCCAGAGAA TAAGGAGCCC AAAGAAAAAA GAAGCAAAAG AGATTATATT
    CAGGAAAAGC AAAGGAGACA AGAAGAGCAG AGGAAAAGAC ATCTGGAGGC TGCTGCTCTG
    CTGGGAGAAA GAAATGCAGA TGGTTTGATT GTGGCCAGTC GTTTCCACCC CACGCCCCTG
    CTGCTGTCTT TGCTGGACTT TGTAGCCCCG TCAAGGCCGT TTGTGGTCTA CTGTCAGTAT
    AAAGAGCCTT TGTTGGAATG CTACACAAAA CTTCGGGAGA GGGGAGGAGT CATTAACCTG
    AGGTTGTCTG AAACCTGGCT CAGAAATTAC CAGGTTCTGC CAGATCGGAG TCATCCCAAA
    TTATTGATGA GTGGCGGTGG AGGGTACCTT CTGTCAGGGT TCACTGTTGT CTCAGACAGC
    CTTCGGGCAG ACCCCAGCCT CAAGTCCTGC ACAGGCGCTT TAGACCCACA CAAGGCTGAG
    GAGCCAGCAG CTAAAAAACA GAAATGCATG GAATCTGCCT CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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