TSC1 cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following TSC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TSC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu18443 NM_000368.5
Latest version!
Homo sapiens TSC complex subunit 1 (TSC1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$399.50-$559.30
$799.00
OHu18443 XM_017015097.1
Latest version!
Homo sapiens TSC complex subunit 1 (TSC1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$399.50-$559.30
$799.00
OHu18443 XM_006717271.1
Latest version!
Homo sapiens TSC complex subunit 1 (TSC1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$399.50-$559.30
$799.00
OHu18443 XM_017015096.1
Latest version!
Homo sapiens TSC complex subunit 1 (TSC1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$399.50-$559.30
$799.00
OHu18443 XM_011518979.2
Latest version!
Homo sapiens TSC complex subunit 1 (TSC1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$399.50-$559.30
$799.00
OHu18443 XM_005272211.1
Latest version!
Homo sapiens TSC complex subunit 1 (TSC1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$399.50-$559.30
$799.00
OHu98884 NM_001362177.2
Latest version!
Homo sapiens TSC complex subunit 1 (TSC1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $559.30
$799.00
OHu98884 XM_017015100.1
Latest version!
Homo sapiens TSC complex subunit 1 (TSC1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $559.30
$799.00
OHu98884 XM_017015099.1
Latest version!
Homo sapiens tuberous sclerosis 1 (TSC1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $559.30
$799.00
OHu98885 XM_017015101.1
Latest version!
Homo sapiens TSC complex subunit 1 (TSC1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu17591 NM_001162427.2
Latest version!
Homo sapiens TSC complex subunit 1 (TSC1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $790.30
$1129.00
OHu17622 NM_001162426.2
Latest version!
Homo sapiens TSC complex subunit 1 (TSC1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $825.30
$1179.00
OHu17622 XM_017015098.1
Latest version!
Homo sapiens TSC complex subunit 1 (TSC1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $825.30
$1179.00
View Old Accession Versions >>
OHu18443 XM_011518979.1 Homo sapiens tuberous sclerosis 1 (TSC1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$399.50-$559.30
$799.00
OHu18443 NM_000368.4 Homo sapiens TSC complex subunit 1 (TSC1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$399.50-$559.30
$799.00
OHu17622 NM_001162426.1 Homo sapiens TSC complex subunit 1 (TSC1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $825.30
$1179.00
OHu98884 NM_001362177.1 Homo sapiens TSC complex subunit 1 (TSC1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $559.30
$799.00
OHu17591 NM_001162427.1 Homo sapiens TSC complex subunit 1 (TSC1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $790.30
$1129.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu18443
    Clone ID Related Accession (Same CDS sequence) NM_000368.5 , XM_006717271.1 , XM_011518979.1 , XM_017015096.1 , XM_011518979.2 , XM_017015097.1 , XM_005272211.1 , NM_000368.4
    Accession Version NM_000368.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-02
    Organism Homo sapiens(human)
    Product hamartin isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC070032.1, AF013168.1, D87683.1 and N63914.1. On Aug 31, 2019 this sequence version replaced NM_000368.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF013168.1, SRR1803617.178097.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
    GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
    CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
    CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
    GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
    AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
    CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
    CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
    CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
    TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
    AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
    ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
    CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
    AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
    CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
    TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
    GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
    TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
    AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
    GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
    GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
    GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
    GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
    TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
    GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
    AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
    TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
    CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
    ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
    CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCGCAC
    AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
    GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
    CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
    CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
    AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTTAGTC TGCAGAAAGA ACAAGCTAGA
    TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
    CAGCTGCAGC ATGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
    GAGGACTGCA GGAACATGAT TGCGGAGCTG CGGATAGAAC TGAAGAAGGC CAACAACAAG
    GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
    GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
    CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
    AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
    AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
    CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
    CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
    GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAA
    GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
    TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
    CCCAGGCCCA GCAGCGCCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
    AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
    AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
    CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
    GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
    CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
    TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
    CATGAACACA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000359.1
    CDS218..3712
    Translation

    Target ORF information:

    RefSeq Version NM_000368.5
    Organism Homo sapiens(human)
    Definition Homo sapiens TSC complex subunit 1 (TSC1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000368.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
    GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
    CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
    CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
    GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
    AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
    CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
    CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
    CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
    TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
    AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
    ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
    CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
    AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
    CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
    TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
    GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
    TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
    AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
    GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
    GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
    GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
    GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
    TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
    GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
    AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
    TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
    CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
    ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
    CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCGCAC
    AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
    GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
    CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
    CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
    AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTTAGTC TGCAGAAAGA ACAAGCTAGA
    TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
    CAGCTGCAGC ATGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
    GAGGACTGCA GGAACATGAT TGCGGAGCTG CGGATAGAAC TGAAGAAGGC CAACAACAAG
    GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
    GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
    CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
    AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
    AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
    CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
    CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
    GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAA
    GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
    TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
    CCCAGGCCCA GCAGCGCCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
    AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
    AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
    CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
    GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
    CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
    TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
    CATGAACACA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18443
    Clone ID Related Accession (Same CDS sequence) NM_000368.5 , XM_006717271.1 , XM_011518979.1 , XM_017015096.1 , XM_011518979.2 , XM_017015097.1 , XM_005272211.1 , NM_000368.4
    Accession Version NM_000368.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-22
    Organism Homo sapiens(human)
    Product hamartin isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC070032.1, AF013168.1, D87683.1 and N63914.1. This sequence is a reference standard in the RefSeqGene project. On Jun 23, 2009 this sequence version replaced NM_000368.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF013168.1, SRR1803611.75508.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
    GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
    CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
    CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
    GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
    AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
    CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
    CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
    CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
    TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
    AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
    ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
    CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
    AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
    CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
    TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
    GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
    TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
    AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
    GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
    GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
    GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
    GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
    TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
    GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
    AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
    TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
    CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
    ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
    CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCGCAC
    AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
    GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
    CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
    CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
    AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTTAGTC TGCAGAAAGA ACAAGCTAGA
    TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
    CAGCTGCAGC ATGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
    GAGGACTGCA GGAACATGAT TGCGGAGCTG CGGATAGAAC TGAAGAAGGC CAACAACAAG
    GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
    GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
    CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
    AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
    AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
    CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
    CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
    GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAA
    GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
    TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
    CCCAGGCCCA GCAGCGCCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
    AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
    AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
    CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
    GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
    CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
    TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
    CATGAACACA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000359.1
    CDS235..3729
    Translation

    Target ORF information:

    RefSeq Version NM_000368.4
    Organism Homo sapiens(human)
    Definition Homo sapiens TSC complex subunit 1 (TSC1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000368.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
    GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
    CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
    CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
    GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
    AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
    CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
    CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
    CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
    TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
    AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
    ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
    CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
    AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
    CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
    TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
    GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
    TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
    AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
    GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
    GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
    GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
    GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
    TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
    GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
    AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
    TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
    CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
    ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
    CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCGCAC
    AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
    GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
    CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
    CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
    AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTTAGTC TGCAGAAAGA ACAAGCTAGA
    TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
    CAGCTGCAGC ATGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
    GAGGACTGCA GGAACATGAT TGCGGAGCTG CGGATAGAAC TGAAGAAGGC CAACAACAAG
    GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
    GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
    CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
    AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
    AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
    CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
    CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
    GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAA
    GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
    TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
    CCCAGGCCCA GCAGCGCCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
    AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
    AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
    CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
    GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
    CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
    TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
    CATGAACACA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18443
    Clone ID Related Accession (Same CDS sequence) NM_000368.5 , XM_006717271.1 , XM_011518979.1 , XM_017015096.1 , XM_011518979.2 , XM_017015097.1 , XM_005272211.1 , NM_000368.4
    Accession Version XM_006717271.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product hamartin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
    GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
    CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
    CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
    GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
    AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
    CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
    CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
    CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
    TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
    AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
    ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
    CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
    AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
    CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
    TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
    GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
    TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
    AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
    GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
    GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
    GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
    GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
    TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
    GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
    AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
    TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
    CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
    ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
    CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCGCAC
    AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
    GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
    CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
    CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
    AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTTAGTC TGCAGAAAGA ACAAGCTAGA
    TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
    CAGCTGCAGC ATGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
    GAGGACTGCA GGAACATGAT TGCGGAGCTG CGGATAGAAC TGAAGAAGGC CAACAACAAG
    GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
    GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
    CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
    AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
    AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
    CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
    CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
    GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAA
    GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
    TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
    CCCAGGCCCA GCAGCGCCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
    AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
    AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
    CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
    GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
    CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
    TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
    CATGAACACA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006717334.1
    CDS215..3709
    Translation

    Target ORF information:

    RefSeq Version XM_006717271.1
    Organism Homo sapiens(human)
    Definition Homo sapiens TSC complex subunit 1 (TSC1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006717271.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
    GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
    CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
    CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
    GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
    AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
    CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
    CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
    CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
    TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
    AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
    ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
    CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
    AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
    CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
    TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
    GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
    TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
    AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
    GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
    GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
    GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
    GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
    TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
    GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
    AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
    TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
    CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
    ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
    CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCGCAC
    AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
    GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
    CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
    CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
    AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTTAGTC TGCAGAAAGA ACAAGCTAGA
    TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
    CAGCTGCAGC ATGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
    GAGGACTGCA GGAACATGAT TGCGGAGCTG CGGATAGAAC TGAAGAAGGC CAACAACAAG
    GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
    GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
    CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
    AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
    AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
    CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
    CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
    GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAA
    GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
    TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
    CCCAGGCCCA GCAGCGCCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
    AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
    AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
    CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
    GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
    CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
    TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
    CATGAACACA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18443
    Clone ID Related Accession (Same CDS sequence) NM_000368.5 , XM_006717271.1 , XM_011518979.1 , XM_017015096.1 , XM_011518979.2 , XM_017015097.1 , XM_005272211.1 , NM_000368.4
    Accession Version XM_011518979.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product hamartin isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
    GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
    CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
    CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
    GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
    AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
    CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
    CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
    CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
    TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
    AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
    ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
    CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
    AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
    CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
    TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
    GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
    TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
    AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
    GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
    GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
    GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
    GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
    TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
    GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
    AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
    TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
    CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
    ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
    CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCGCAC
    AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
    GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
    CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
    CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
    AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTTAGTC TGCAGAAAGA ACAAGCTAGA
    TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
    CAGCTGCAGC ATGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
    GAGGACTGCA GGAACATGAT TGCGGAGCTG CGGATAGAAC TGAAGAAGGC CAACAACAAG
    GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
    GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
    CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
    AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
    AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
    CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
    CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
    GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAA
    GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
    TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
    CCCAGGCCCA GCAGCGCCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
    AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
    AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
    CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
    GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
    CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
    TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
    CATGAACACA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011517281.1
    Translation

    Target ORF information:

    RefSeq Version XM_011518979.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens tuberous sclerosis 1 (TSC1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011518979.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
    GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
    CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
    CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
    GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
    AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
    CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
    CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
    CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
    TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
    AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
    ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
    CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
    AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
    CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
    TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
    GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
    TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
    AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
    GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
    GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
    GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
    GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
    TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
    GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
    AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
    TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
    CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
    ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
    CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCGCAC
    AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
    GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
    CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
    CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
    AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTTAGTC TGCAGAAAGA ACAAGCTAGA
    TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
    CAGCTGCAGC ATGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
    GAGGACTGCA GGAACATGAT TGCGGAGCTG CGGATAGAAC TGAAGAAGGC CAACAACAAG
    GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
    GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
    CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
    AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
    AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
    CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
    CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
    GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAA
    GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
    TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
    CCCAGGCCCA GCAGCGCCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
    AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
    AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
    CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
    GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
    CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
    TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
    CATGAACACA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18443
    Clone ID Related Accession (Same CDS sequence) NM_000368.5 , XM_006717271.1 , XM_011518979.1 , XM_017015096.1 , XM_011518979.2 , XM_017015097.1 , XM_005272211.1 , NM_000368.4
    Accession Version XM_017015096.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product hamartin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
    GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
    CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
    CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
    GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
    AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
    CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
    CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
    CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
    TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
    AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
    ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
    CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
    AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
    CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
    TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
    GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
    TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
    AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
    GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
    GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
    GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
    GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
    TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
    GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
    AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
    TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
    CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
    ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
    CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCGCAC
    AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
    GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
    CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
    CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
    AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTTAGTC TGCAGAAAGA ACAAGCTAGA
    TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
    CAGCTGCAGC ATGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
    GAGGACTGCA GGAACATGAT TGCGGAGCTG CGGATAGAAC TGAAGAAGGC CAACAACAAG
    GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
    GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
    CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
    AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
    AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
    CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
    CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
    GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAA
    GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
    TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
    CCCAGGCCCA GCAGCGCCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
    AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
    AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
    CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
    GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
    CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
    TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
    CATGAACACA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016870585.1
    CDS694..4188
    Translation

    Target ORF information:

    RefSeq Version XM_017015096.1
    Organism Homo sapiens(human)
    Definition Homo sapiens TSC complex subunit 1 (TSC1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017015096.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
    GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
    CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
    CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
    GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
    AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
    CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
    CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
    CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
    TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
    AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
    ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
    CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
    AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
    CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
    TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
    GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
    TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
    AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
    GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
    GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
    GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
    GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
    TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
    GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
    AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
    TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
    CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
    ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
    CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCGCAC
    AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
    GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
    CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
    CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
    AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTTAGTC TGCAGAAAGA ACAAGCTAGA
    TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
    CAGCTGCAGC ATGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
    GAGGACTGCA GGAACATGAT TGCGGAGCTG CGGATAGAAC TGAAGAAGGC CAACAACAAG
    GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
    GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
    CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
    AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
    AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
    CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
    CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
    GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAA
    GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
    TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
    CCCAGGCCCA GCAGCGCCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
    AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
    AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
    CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
    GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
    CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
    TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
    CATGAACACA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18443
    Clone ID Related Accession (Same CDS sequence) NM_000368.5 , XM_006717271.1 , XM_011518979.1 , XM_017015096.1 , XM_011518979.2 , XM_017015097.1 , XM_005272211.1 , NM_000368.4
    Accession Version XM_011518979.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product hamartin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518979.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
    GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
    CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
    CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
    GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
    AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
    CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
    CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
    CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
    TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
    AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
    ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
    CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
    AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
    CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
    TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
    GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
    TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
    AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
    GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
    GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
    GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
    GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
    TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
    GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
    AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
    TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
    CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
    ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
    CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCGCAC
    AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
    GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
    CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
    CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
    AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTTAGTC TGCAGAAAGA ACAAGCTAGA
    TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
    CAGCTGCAGC ATGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
    GAGGACTGCA GGAACATGAT TGCGGAGCTG CGGATAGAAC TGAAGAAGGC CAACAACAAG
    GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
    GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
    CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
    AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
    AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
    CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
    CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
    GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAA
    GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
    TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
    CCCAGGCCCA GCAGCGCCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
    AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
    AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
    CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
    GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
    CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
    TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
    CATGAACACA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011517281.1
    CDS229..3723
    Translation

    Target ORF information:

    RefSeq Version XM_011518979.2
    Organism Homo sapiens(human)
    Definition Homo sapiens TSC complex subunit 1 (TSC1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011518979.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
    GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
    CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
    CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
    GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
    AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
    CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
    CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
    CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
    TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
    AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
    ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
    CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
    AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
    CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
    TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
    GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
    TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
    AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
    GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
    GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
    GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
    GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
    TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
    GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
    AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
    TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
    CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
    ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
    CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCGCAC
    AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
    GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
    CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
    CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
    AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTTAGTC TGCAGAAAGA ACAAGCTAGA
    TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
    CAGCTGCAGC ATGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
    GAGGACTGCA GGAACATGAT TGCGGAGCTG CGGATAGAAC TGAAGAAGGC CAACAACAAG
    GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
    GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
    CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
    AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
    AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
    CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
    CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
    GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAA
    GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
    TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
    CCCAGGCCCA GCAGCGCCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
    AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
    AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
    CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
    GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
    CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
    TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
    CATGAACACA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18443
    Clone ID Related Accession (Same CDS sequence) NM_000368.5 , XM_006717271.1 , XM_011518979.1 , XM_017015096.1 , XM_011518979.2 , XM_017015097.1 , XM_005272211.1 , NM_000368.4
    Accession Version XM_017015097.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product hamartin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
    GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
    CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
    CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
    GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
    AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
    CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
    CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
    CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
    TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
    AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
    ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
    CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
    AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
    CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
    TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
    GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
    TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
    AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
    GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
    GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
    GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
    GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
    TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
    GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
    AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
    TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
    CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
    ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
    CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCGCAC
    AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
    GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
    CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
    CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
    AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTTAGTC TGCAGAAAGA ACAAGCTAGA
    TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
    CAGCTGCAGC ATGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
    GAGGACTGCA GGAACATGAT TGCGGAGCTG CGGATAGAAC TGAAGAAGGC CAACAACAAG
    GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
    GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
    CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
    AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
    AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
    CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
    CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
    GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAA
    GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
    TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
    CCCAGGCCCA GCAGCGCCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
    AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
    AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
    CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
    GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
    CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
    TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
    CATGAACACA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016870586.1
    CDS665..4159
    Translation

    Target ORF information:

    RefSeq Version XM_017015097.1
    Organism Homo sapiens(human)
    Definition Homo sapiens TSC complex subunit 1 (TSC1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017015097.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
    GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
    CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
    CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
    GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
    AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
    CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
    CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
    CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
    TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
    AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
    ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
    CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
    AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
    CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
    TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
    GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
    TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
    AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
    GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
    GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
    GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
    GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
    TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
    GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
    AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
    TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
    CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
    ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
    CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCGCAC
    AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
    GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
    CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
    CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
    AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTTAGTC TGCAGAAAGA ACAAGCTAGA
    TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
    CAGCTGCAGC ATGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
    GAGGACTGCA GGAACATGAT TGCGGAGCTG CGGATAGAAC TGAAGAAGGC CAACAACAAG
    GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
    GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
    CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
    AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
    AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
    CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
    CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
    GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAA
    GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
    TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
    CCCAGGCCCA GCAGCGCCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
    AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
    AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
    CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
    GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
    CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
    TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
    CATGAACACA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18443
    Clone ID Related Accession (Same CDS sequence) NM_000368.5 , XM_006717271.1 , XM_011518979.1 , XM_017015096.1 , XM_011518979.2 , XM_017015097.1 , XM_005272211.1 , NM_000368.4
    Accession Version XM_005272211.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product hamartin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
    GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
    CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
    CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
    GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
    AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
    CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
    CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
    CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
    TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
    AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
    ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
    CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
    AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
    CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
    TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
    GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
    TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
    AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
    GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
    GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
    GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
    GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
    TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
    GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
    AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
    TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
    CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
    ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
    CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCGCAC
    AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
    GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
    CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
    CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
    AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTTAGTC TGCAGAAAGA ACAAGCTAGA
    TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
    CAGCTGCAGC ATGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
    GAGGACTGCA GGAACATGAT TGCGGAGCTG CGGATAGAAC TGAAGAAGGC CAACAACAAG
    GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
    GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
    CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
    AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
    AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
    CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
    CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
    GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAA
    GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
    TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
    CCCAGGCCCA GCAGCGCCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
    AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
    AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
    CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
    GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
    CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
    TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
    CATGAACACA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005272268.1
    CDS280..3774
    Translation

    Target ORF information:

    RefSeq Version XM_005272211.1
    Organism Homo sapiens(human)
    Definition Homo sapiens TSC complex subunit 1 (TSC1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005272211.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
    GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
    CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
    CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
    GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
    AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
    CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
    CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
    CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
    TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
    AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
    ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
    CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
    AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
    CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
    TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
    GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
    TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
    AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
    GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
    GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
    GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
    GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
    TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
    GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
    AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
    TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
    CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
    ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
    CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCGCAC
    AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
    GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
    CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
    CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
    AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTTAGTC TGCAGAAAGA ACAAGCTAGA
    TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
    CAGCTGCAGC ATGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
    GAGGACTGCA GGAACATGAT TGCGGAGCTG CGGATAGAAC TGAAGAAGGC CAACAACAAG
    GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
    GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
    CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
    AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
    AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
    CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
    CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
    GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAA
    GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
    TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
    CCCAGGCCCA GCAGCGCCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
    AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
    AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
    CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
    GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
    CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
    TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
    CATGAACACA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu98884
    Clone ID Related Accession (Same CDS sequence) XM_017015099.1 , NM_001362177.1 , NM_001362177.2 , XM_017015100.1
    Accession Version NM_001362177.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3132bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-25
    Organism Homo sapiens(human)
    Product hamartin isoform 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL445645.10 and N63914.1. On Apr 6, 2018 this sequence version replaced XM_017015099.1. Transcript Variant: This variant (5) lacks a 5' exon which results in the use of a downstream AUG compared to variant 1. The encoded isoform (5) is shorter and has a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.256316.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGACACTG ACGTCGTTGT CCTCACAACA GGCGTCTTGG TGTTGATAAC CATGCTACCA 
    ATGATTCCAC AGTCTGGGAA ACAGCATCTT CTTGATTTCT TTGACATTTT TGGCCGTCTG
    TCATCATGGT GCCTGAAGAA ACCAGGCCAC GTGGCGGAAG TCTATCTCGT CCATCTCCAT
    GCCAGTGTGT ACGCACTCTT TCATCGCCTT TATGGAATGT ACCCTTGCAA CTTCGTCTCC
    TTTTTGCGTT CTCATTACAG TATGAAAGAA AACCTGGAGA CTTTTGAAGA AGTGGTCAAG
    CCAATGATGG AGCATGTGCG AATTCATCCG GAATTAGTGA CTGGATCCAA GGACCATGAA
    CTGGACCCTC GAAGGTGGAA GAGATTAGAA ACTCATGATG TTGTGATCGA GTGTGCCAAA
    ATCTCTCTGG ATCCCACAGA AGCCTCATAT GAAGATGGCT ATTCTGTGTC TCACCAAATC
    TCAGCCCGCT TTCCTCATCG TTCAGCCGAT GTCACCACCA GCCCTTATGC TGACACACAG
    AATAGCTATG GGTGTGCTAC TTCTACCCCT TACTCCACGT CTCGGCTGAT GTTGTTAAAT
    ATGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCATCGA CACGGCTGAT AACTGAACCA
    CCACAAGCTA CTCTTTGGAG CCCATCTATG GTTTGTGGTA TGACCACTCC TCCAACTTCT
    CCTGGAAATG TCCCACCTGA TCTGTCACAC CCTTACAGTA AAGTCTTTGG TACAACTGCA
    GGTGGAAAAG GAACTCCTCT GGGAACCCCA GCAACCTCTC CTCCTCCAGC CCCACTCTGT
    CATTCGGATG ACTACGTGCA CATTTCACTC CCCCAGGCCA CAGTCACACC CCCCAGGAAG
    GAAGAGAGAA TGGATTCTGC AAGACCATGT CTACACAGAC AACACCATCT TCTGAATGAC
    AGAGGATCAG AAGAGCCACC TGGCAGCAAA GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG
    TTTTTAGGTG ATCTGGCCTC TGAAGAAGAT AGTATTGAAA AAGATAAAGA AGAAGCTGCA
    ATATCTAGAG AACTTTCTGA GATCACCACA GCAGAGGCAG AGCCTGTGGT TCCTCGAGGA
    GGCTTTGACT CTCCCTTTTA CCGAGACAGT CTCCCAGGTT CTCAGCGGAA GACCCACTCG
    GCAGCCTCCA GTTCTCAGGG CGCCAGCGTG AACCCTGAGC CTTTACACTC CTCCCTGGAC
    AAGCTTGGGC CTGACACACC AAAGCAAGCC TTTACTCCCA TAGACCTGCC CTGCGGCAGT
    GCTGATGAAA GCCCTGCGGG AGACAGGGAA TGCCAGACTT CTTTGGAGAC CAGTATCTTC
    ACTCCCAGTC CTTGTAAAAT TCCACCTCCG ACGAGAGTGG GCTTTGGAAG CGGGCAGCCT
    CCCCCGTATG ATCATCTTTT TGAGGTGGCA TTGCCAAAGA CAGCCCATCA TTTTGTCATC
    AGGAAGACTG AGGAGCTGTT AAAGAAAGCA AAAGGAAACA CAGAGGAAGA TGGTGTGCCC
    TCTACCTCCC CAATGGAAGT GCTGGACAGA CTGATACAGC AGGGAGCAGA CGCGCACAGC
    AAGGAGCTGA ACAAGTTGCC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTTGGAGGC
    TCTCCTCCTT CAGATGAGAT CCGCACCCTC CGAGACCAGT TGCTTTTACT GCACAACCAG
    TTACTCTATG AGCGTTTTAA GAGGCAGCAG CATGCCCTCC GGAACAGGCG GCTCCTCCGC
    AAGGTGATCA AAGCAGCAGC TCTGGAGGAA CATAATGCTG CCATGAAAGA TCAGTTGAAG
    TTACAAGAGA AGGACATCCA GATGTGGAAG GTTAGTCTGC AGAAAGAACA AGCTAGATAC
    AATCAGCTCC AGGAGCAGCG TGACACTATG GTAACCAAGC TCCACAGCCA GATCAGACAG
    CTGCAGCATG ACCGAGAGGA ATTCTACAAC CAGAGCCAGG AATTACAGAC GAAGCTGGAG
    GACTGCAGGA ACATGATTGC GGAGCTGCGG ATAGAACTGA AGAAGGCCAA CAACAAGGTG
    TGTCACACTG AGCTGCTGCT CAGTCAGGTT TCCCAAAAGC TCTCAAACAG TGAGTCGGTC
    CAGCAGCAGA TGGAGTTCTT GAACAGGCAG CTGTTGGTTC TTGGGGAGGT CAACGAGCTC
    TATTTGGAAC AACTGCAGAA CAAGCACTCA GATACCACAA AGGAAGTAGA AATGATGAAA
    GCCGCCTATC GGAAAGAGCT AGAAAAAAAC AGAAGCCATG TTCTCCAGCA GACTCAGAGG
    CTTGATACCT CCCAAAAACG GATTTTGGAA CTGGAATCTC ACCTGGCCAA GAAAGACCAC
    CTTCTTTTGG AACAGAAGAA ATATCTAGAG GATGTCAAAC TCCAGGCAAG AGGACAGCTG
    CAGGCCGCAG AGAGCAGGTA TGAGGCTCAG AAAAGGATAA CCCAGGTGTT TGAATTGGAG
    ATCTTAGATT TATATGGCAG GTTGGAGAAA GATGGCCTCC TGAAAAAACT TGAAGAAGAA
    AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA AGGCTTGACT GTTGTAATGA CGGGTGCTCA
    GATTCCATGG TAGGGCACAA TGAAGAGGCA TCTGGCCACA ACGGTGAGAC CAAGACCCCC
    AGGCCCAGCA GCGCCCGGGG CAGTAGTGGA AGCAGAGGTG GTGGAGGCAG CAGCAGCAGC
    AGCAGCGAGC TTTCTACCCC AGAGAAACCC CCACACCAGA GGGCAGGCCC ATTCAGCAGT
    CGGTGGGAGA CGACTATGGG AGAAGCGTCT GCCAGCATCC CCACCACTGT GGGCTCACTT
    CCCAGTTCAA AAAGCTTCCT GGGTATGAAG GCTCGAGAGT TATTTCGTAA TAAGAGCGAG
    AGCCAGTGTG ATGAGGACGG CATGACCAGT AGCCTTTCTG AGAGCCTAAA GACAGAACTG
    GGCAAAGACT TGGGTGTGGA AGCCAAGATT CCCCTGAACC TAGATGGCCC TCACCCGTCT
    CCCCCGACCC CGGACAGTGT TGGACAGCTA CATATCATGG ACTACAATGA GACTCATCAT
    GAACACAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001349106.1
    CDS494..3625
    Translation

    Target ORF information:

    RefSeq Version NM_001362177.1
    Organism Homo sapiens(human)
    Definition Homo sapiens TSC complex subunit 1 (TSC1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001362177.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGACACTG ACGTCGTTGT CCTCACAACA GGCGTCTTGG TGTTGATAAC CATGCTACCA 
    ATGATTCCAC AGTCTGGGAA ACAGCATCTT CTTGATTTCT TTGACATTTT TGGCCGTCTG
    TCATCATGGT GCCTGAAGAA ACCAGGCCAC GTGGCGGAAG TCTATCTCGT CCATCTCCAT
    GCCAGTGTGT ACGCACTCTT TCATCGCCTT TATGGAATGT ACCCTTGCAA CTTCGTCTCC
    TTTTTGCGTT CTCATTACAG TATGAAAGAA AACCTGGAGA CTTTTGAAGA AGTGGTCAAG
    CCAATGATGG AGCATGTGCG AATTCATCCG GAATTAGTGA CTGGATCCAA GGACCATGAA
    CTGGACCCTC GAAGGTGGAA GAGATTAGAA ACTCATGATG TTGTGATCGA GTGTGCCAAA
    ATCTCTCTGG ATCCCACAGA AGCCTCATAT GAAGATGGCT ATTCTGTGTC TCACCAAATC
    TCAGCCCGCT TTCCTCATCG TTCAGCCGAT GTCACCACCA GCCCTTATGC TGACACACAG
    AATAGCTATG GGTGTGCTAC TTCTACCCCT TACTCCACGT CTCGGCTGAT GTTGTTAAAT
    ATGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCATCGA CACGGCTGAT AACTGAACCA
    CCACAAGCTA CTCTTTGGAG CCCATCTATG GTTTGTGGTA TGACCACTCC TCCAACTTCT
    CCTGGAAATG TCCCACCTGA TCTGTCACAC CCTTACAGTA AAGTCTTTGG TACAACTGCA
    GGTGGAAAAG GAACTCCTCT GGGAACCCCA GCAACCTCTC CTCCTCCAGC CCCACTCTGT
    CATTCGGATG ACTACGTGCA CATTTCACTC CCCCAGGCCA CAGTCACACC CCCCAGGAAG
    GAAGAGAGAA TGGATTCTGC AAGACCATGT CTACACAGAC AACACCATCT TCTGAATGAC
    AGAGGATCAG AAGAGCCACC TGGCAGCAAA GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG
    TTTTTAGGTG ATCTGGCCTC TGAAGAAGAT AGTATTGAAA AAGATAAAGA AGAAGCTGCA
    ATATCTAGAG AACTTTCTGA GATCACCACA GCAGAGGCAG AGCCTGTGGT TCCTCGAGGA
    GGCTTTGACT CTCCCTTTTA CCGAGACAGT CTCCCAGGTT CTCAGCGGAA GACCCACTCG
    GCAGCCTCCA GTTCTCAGGG CGCCAGCGTG AACCCTGAGC CTTTACACTC CTCCCTGGAC
    AAGCTTGGGC CTGACACACC AAAGCAAGCC TTTACTCCCA TAGACCTGCC CTGCGGCAGT
    GCTGATGAAA GCCCTGCGGG AGACAGGGAA TGCCAGACTT CTTTGGAGAC CAGTATCTTC
    ACTCCCAGTC CTTGTAAAAT TCCACCTCCG ACGAGAGTGG GCTTTGGAAG CGGGCAGCCT
    CCCCCGTATG ATCATCTTTT TGAGGTGGCA TTGCCAAAGA CAGCCCATCA TTTTGTCATC
    AGGAAGACTG AGGAGCTGTT AAAGAAAGCA AAAGGAAACA CAGAGGAAGA TGGTGTGCCC
    TCTACCTCCC CAATGGAAGT GCTGGACAGA CTGATACAGC AGGGAGCAGA CGCGCACAGC
    AAGGAGCTGA ACAAGTTGCC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTTGGAGGC
    TCTCCTCCTT CAGATGAGAT CCGCACCCTC CGAGACCAGT TGCTTTTACT GCACAACCAG
    TTACTCTATG AGCGTTTTAA GAGGCAGCAG CATGCCCTCC GGAACAGGCG GCTCCTCCGC
    AAGGTGATCA AAGCAGCAGC TCTGGAGGAA CATAATGCTG CCATGAAAGA TCAGTTGAAG
    TTACAAGAGA AGGACATCCA GATGTGGAAG GTTAGTCTGC AGAAAGAACA AGCTAGATAC
    AATCAGCTCC AGGAGCAGCG TGACACTATG GTAACCAAGC TCCACAGCCA GATCAGACAG
    CTGCAGCATG ACCGAGAGGA ATTCTACAAC CAGAGCCAGG AATTACAGAC GAAGCTGGAG
    GACTGCAGGA ACATGATTGC GGAGCTGCGG ATAGAACTGA AGAAGGCCAA CAACAAGGTG
    TGTCACACTG AGCTGCTGCT CAGTCAGGTT TCCCAAAAGC TCTCAAACAG TGAGTCGGTC
    CAGCAGCAGA TGGAGTTCTT GAACAGGCAG CTGTTGGTTC TTGGGGAGGT CAACGAGCTC
    TATTTGGAAC AACTGCAGAA CAAGCACTCA GATACCACAA AGGAAGTAGA AATGATGAAA
    GCCGCCTATC GGAAAGAGCT AGAAAAAAAC AGAAGCCATG TTCTCCAGCA GACTCAGAGG
    CTTGATACCT CCCAAAAACG GATTTTGGAA CTGGAATCTC ACCTGGCCAA GAAAGACCAC
    CTTCTTTTGG AACAGAAGAA ATATCTAGAG GATGTCAAAC TCCAGGCAAG AGGACAGCTG
    CAGGCCGCAG AGAGCAGGTA TGAGGCTCAG AAAAGGATAA CCCAGGTGTT TGAATTGGAG
    ATCTTAGATT TATATGGCAG GTTGGAGAAA GATGGCCTCC TGAAAAAACT TGAAGAAGAA
    AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA AGGCTTGACT GTTGTAATGA CGGGTGCTCA
    GATTCCATGG TAGGGCACAA TGAAGAGGCA TCTGGCCACA ACGGTGAGAC CAAGACCCCC
    AGGCCCAGCA GCGCCCGGGG CAGTAGTGGA AGCAGAGGTG GTGGAGGCAG CAGCAGCAGC
    AGCAGCGAGC TTTCTACCCC AGAGAAACCC CCACACCAGA GGGCAGGCCC ATTCAGCAGT
    CGGTGGGAGA CGACTATGGG AGAAGCGTCT GCCAGCATCC CCACCACTGT GGGCTCACTT
    CCCAGTTCAA AAAGCTTCCT GGGTATGAAG GCTCGAGAGT TATTTCGTAA TAAGAGCGAG
    AGCCAGTGTG ATGAGGACGG CATGACCAGT AGCCTTTCTG AGAGCCTAAA GACAGAACTG
    GGCAAAGACT TGGGTGTGGA AGCCAAGATT CCCCTGAACC TAGATGGCCC TCACCCGTCT
    CCCCCGACCC CGGACAGTGT TGGACAGCTA CATATCATGG ACTACAATGA GACTCATCAT
    GAACACAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu98884
    Clone ID Related Accession (Same CDS sequence) XM_017015099.1 , NM_001362177.1 , NM_001362177.2 , XM_017015100.1
    Accession Version NM_001362177.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3132bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-25
    Organism Homo sapiens(human)
    Product hamartin isoform 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL445645.10 and N63914.1. On Jun 2, 2019 this sequence version replaced NM_001362177.1. Transcript Variant: This variant (5) lacks a 5' exon which results in the use of a downstream AUG compared to variant 1. The encoded isoform (5) is shorter and has a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.256316.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGACACTG ACGTCGTTGT CCTCACAACA GGCGTCTTGG TGTTGATAAC CATGCTACCA 
    ATGATTCCAC AGTCTGGGAA ACAGCATCTT CTTGATTTCT TTGACATTTT TGGCCGTCTG
    TCATCATGGT GCCTGAAGAA ACCAGGCCAC GTGGCGGAAG TCTATCTCGT CCATCTCCAT
    GCCAGTGTGT ACGCACTCTT TCATCGCCTT TATGGAATGT ACCCTTGCAA CTTCGTCTCC
    TTTTTGCGTT CTCATTACAG TATGAAAGAA AACCTGGAGA CTTTTGAAGA AGTGGTCAAG
    CCAATGATGG AGCATGTGCG AATTCATCCG GAATTAGTGA CTGGATCCAA GGACCATGAA
    CTGGACCCTC GAAGGTGGAA GAGATTAGAA ACTCATGATG TTGTGATCGA GTGTGCCAAA
    ATCTCTCTGG ATCCCACAGA AGCCTCATAT GAAGATGGCT ATTCTGTGTC TCACCAAATC
    TCAGCCCGCT TTCCTCATCG TTCAGCCGAT GTCACCACCA GCCCTTATGC TGACACACAG
    AATAGCTATG GGTGTGCTAC TTCTACCCCT TACTCCACGT CTCGGCTGAT GTTGTTAAAT
    ATGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCATCGA CACGGCTGAT AACTGAACCA
    CCACAAGCTA CTCTTTGGAG CCCATCTATG GTTTGTGGTA TGACCACTCC TCCAACTTCT
    CCTGGAAATG TCCCACCTGA TCTGTCACAC CCTTACAGTA AAGTCTTTGG TACAACTGCA
    GGTGGAAAAG GAACTCCTCT GGGAACCCCA GCAACCTCTC CTCCTCCAGC CCCACTCTGT
    CATTCGGATG ACTACGTGCA CATTTCACTC CCCCAGGCCA CAGTCACACC CCCCAGGAAG
    GAAGAGAGAA TGGATTCTGC AAGACCATGT CTACACAGAC AACACCATCT TCTGAATGAC
    AGAGGATCAG AAGAGCCACC TGGCAGCAAA GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG
    TTTTTAGGTG ATCTGGCCTC TGAAGAAGAT AGTATTGAAA AAGATAAAGA AGAAGCTGCA
    ATATCTAGAG AACTTTCTGA GATCACCACA GCAGAGGCAG AGCCTGTGGT TCCTCGAGGA
    GGCTTTGACT CTCCCTTTTA CCGAGACAGT CTCCCAGGTT CTCAGCGGAA GACCCACTCG
    GCAGCCTCCA GTTCTCAGGG CGCCAGCGTG AACCCTGAGC CTTTACACTC CTCCCTGGAC
    AAGCTTGGGC CTGACACACC AAAGCAAGCC TTTACTCCCA TAGACCTGCC CTGCGGCAGT
    GCTGATGAAA GCCCTGCGGG AGACAGGGAA TGCCAGACTT CTTTGGAGAC CAGTATCTTC
    ACTCCCAGTC CTTGTAAAAT TCCACCTCCG ACGAGAGTGG GCTTTGGAAG CGGGCAGCCT
    CCCCCGTATG ATCATCTTTT TGAGGTGGCA TTGCCAAAGA CAGCCCATCA TTTTGTCATC
    AGGAAGACTG AGGAGCTGTT AAAGAAAGCA AAAGGAAACA CAGAGGAAGA TGGTGTGCCC
    TCTACCTCCC CAATGGAAGT GCTGGACAGA CTGATACAGC AGGGAGCAGA CGCGCACAGC
    AAGGAGCTGA ACAAGTTGCC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTTGGAGGC
    TCTCCTCCTT CAGATGAGAT CCGCACCCTC CGAGACCAGT TGCTTTTACT GCACAACCAG
    TTACTCTATG AGCGTTTTAA GAGGCAGCAG CATGCCCTCC GGAACAGGCG GCTCCTCCGC
    AAGGTGATCA AAGCAGCAGC TCTGGAGGAA CATAATGCTG CCATGAAAGA TCAGTTGAAG
    TTACAAGAGA AGGACATCCA GATGTGGAAG GTTAGTCTGC AGAAAGAACA AGCTAGATAC
    AATCAGCTCC AGGAGCAGCG TGACACTATG GTAACCAAGC TCCACAGCCA GATCAGACAG
    CTGCAGCATG ACCGAGAGGA ATTCTACAAC CAGAGCCAGG AATTACAGAC GAAGCTGGAG
    GACTGCAGGA ACATGATTGC GGAGCTGCGG ATAGAACTGA AGAAGGCCAA CAACAAGGTG
    TGTCACACTG AGCTGCTGCT CAGTCAGGTT TCCCAAAAGC TCTCAAACAG TGAGTCGGTC
    CAGCAGCAGA TGGAGTTCTT GAACAGGCAG CTGTTGGTTC TTGGGGAGGT CAACGAGCTC
    TATTTGGAAC AACTGCAGAA CAAGCACTCA GATACCACAA AGGAAGTAGA AATGATGAAA
    GCCGCCTATC GGAAAGAGCT AGAAAAAAAC AGAAGCCATG TTCTCCAGCA GACTCAGAGG
    CTTGATACCT CCCAAAAACG GATTTTGGAA CTGGAATCTC ACCTGGCCAA GAAAGACCAC
    CTTCTTTTGG AACAGAAGAA ATATCTAGAG GATGTCAAAC TCCAGGCAAG AGGACAGCTG
    CAGGCCGCAG AGAGCAGGTA TGAGGCTCAG AAAAGGATAA CCCAGGTGTT TGAATTGGAG
    ATCTTAGATT TATATGGCAG GTTGGAGAAA GATGGCCTCC TGAAAAAACT TGAAGAAGAA
    AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA AGGCTTGACT GTTGTAATGA CGGGTGCTCA
    GATTCCATGG TAGGGCACAA TGAAGAGGCA TCTGGCCACA ACGGTGAGAC CAAGACCCCC
    AGGCCCAGCA GCGCCCGGGG CAGTAGTGGA AGCAGAGGTG GTGGAGGCAG CAGCAGCAGC
    AGCAGCGAGC TTTCTACCCC AGAGAAACCC CCACACCAGA GGGCAGGCCC ATTCAGCAGT
    CGGTGGGAGA CGACTATGGG AGAAGCGTCT GCCAGCATCC CCACCACTGT GGGCTCACTT
    CCCAGTTCAA AAAGCTTCCT GGGTATGAAG GCTCGAGAGT TATTTCGTAA TAAGAGCGAG
    AGCCAGTGTG ATGAGGACGG CATGACCAGT AGCCTTTCTG AGAGCCTAAA GACAGAACTG
    GGCAAAGACT TGGGTGTGGA AGCCAAGATT CCCCTGAACC TAGATGGCCC TCACCCGTCT
    CCCCCGACCC CGGACAGTGT TGGACAGCTA CATATCATGG ACTACAATGA GACTCATCAT
    GAACACAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001349106.1
    CDS477..3608
    Translation

    Target ORF information:

    RefSeq Version NM_001362177.2
    Organism Homo sapiens(human)
    Definition Homo sapiens TSC complex subunit 1 (TSC1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001362177.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGACACTG ACGTCGTTGT CCTCACAACA GGCGTCTTGG TGTTGATAAC CATGCTACCA 
    ATGATTCCAC AGTCTGGGAA ACAGCATCTT CTTGATTTCT TTGACATTTT TGGCCGTCTG
    TCATCATGGT GCCTGAAGAA ACCAGGCCAC GTGGCGGAAG TCTATCTCGT CCATCTCCAT
    GCCAGTGTGT ACGCACTCTT TCATCGCCTT TATGGAATGT ACCCTTGCAA CTTCGTCTCC
    TTTTTGCGTT CTCATTACAG TATGAAAGAA AACCTGGAGA CTTTTGAAGA AGTGGTCAAG
    CCAATGATGG AGCATGTGCG AATTCATCCG GAATTAGTGA CTGGATCCAA GGACCATGAA
    CTGGACCCTC GAAGGTGGAA GAGATTAGAA ACTCATGATG TTGTGATCGA GTGTGCCAAA
    ATCTCTCTGG ATCCCACAGA AGCCTCATAT GAAGATGGCT ATTCTGTGTC TCACCAAATC
    TCAGCCCGCT TTCCTCATCG TTCAGCCGAT GTCACCACCA GCCCTTATGC TGACACACAG
    AATAGCTATG GGTGTGCTAC TTCTACCCCT TACTCCACGT CTCGGCTGAT GTTGTTAAAT
    ATGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCATCGA CACGGCTGAT AACTGAACCA
    CCACAAGCTA CTCTTTGGAG CCCATCTATG GTTTGTGGTA TGACCACTCC TCCAACTTCT
    CCTGGAAATG TCCCACCTGA TCTGTCACAC CCTTACAGTA AAGTCTTTGG TACAACTGCA
    GGTGGAAAAG GAACTCCTCT GGGAACCCCA GCAACCTCTC CTCCTCCAGC CCCACTCTGT
    CATTCGGATG ACTACGTGCA CATTTCACTC CCCCAGGCCA CAGTCACACC CCCCAGGAAG
    GAAGAGAGAA TGGATTCTGC AAGACCATGT CTACACAGAC AACACCATCT TCTGAATGAC
    AGAGGATCAG AAGAGCCACC TGGCAGCAAA GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG
    TTTTTAGGTG ATCTGGCCTC TGAAGAAGAT AGTATTGAAA AAGATAAAGA AGAAGCTGCA
    ATATCTAGAG AACTTTCTGA GATCACCACA GCAGAGGCAG AGCCTGTGGT TCCTCGAGGA
    GGCTTTGACT CTCCCTTTTA CCGAGACAGT CTCCCAGGTT CTCAGCGGAA GACCCACTCG
    GCAGCCTCCA GTTCTCAGGG CGCCAGCGTG AACCCTGAGC CTTTACACTC CTCCCTGGAC
    AAGCTTGGGC CTGACACACC AAAGCAAGCC TTTACTCCCA TAGACCTGCC CTGCGGCAGT
    GCTGATGAAA GCCCTGCGGG AGACAGGGAA TGCCAGACTT CTTTGGAGAC CAGTATCTTC
    ACTCCCAGTC CTTGTAAAAT TCCACCTCCG ACGAGAGTGG GCTTTGGAAG CGGGCAGCCT
    CCCCCGTATG ATCATCTTTT TGAGGTGGCA TTGCCAAAGA CAGCCCATCA TTTTGTCATC
    AGGAAGACTG AGGAGCTGTT AAAGAAAGCA AAAGGAAACA CAGAGGAAGA TGGTGTGCCC
    TCTACCTCCC CAATGGAAGT GCTGGACAGA CTGATACAGC AGGGAGCAGA CGCGCACAGC
    AAGGAGCTGA ACAAGTTGCC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTTGGAGGC
    TCTCCTCCTT CAGATGAGAT CCGCACCCTC CGAGACCAGT TGCTTTTACT GCACAACCAG
    TTACTCTATG AGCGTTTTAA GAGGCAGCAG CATGCCCTCC GGAACAGGCG GCTCCTCCGC
    AAGGTGATCA AAGCAGCAGC TCTGGAGGAA CATAATGCTG CCATGAAAGA TCAGTTGAAG
    TTACAAGAGA AGGACATCCA GATGTGGAAG GTTAGTCTGC AGAAAGAACA AGCTAGATAC
    AATCAGCTCC AGGAGCAGCG TGACACTATG GTAACCAAGC TCCACAGCCA GATCAGACAG
    CTGCAGCATG ACCGAGAGGA ATTCTACAAC CAGAGCCAGG AATTACAGAC GAAGCTGGAG
    GACTGCAGGA ACATGATTGC GGAGCTGCGG ATAGAACTGA AGAAGGCCAA CAACAAGGTG
    TGTCACACTG AGCTGCTGCT CAGTCAGGTT TCCCAAAAGC TCTCAAACAG TGAGTCGGTC
    CAGCAGCAGA TGGAGTTCTT GAACAGGCAG CTGTTGGTTC TTGGGGAGGT CAACGAGCTC
    TATTTGGAAC AACTGCAGAA CAAGCACTCA GATACCACAA AGGAAGTAGA AATGATGAAA
    GCCGCCTATC GGAAAGAGCT AGAAAAAAAC AGAAGCCATG TTCTCCAGCA GACTCAGAGG
    CTTGATACCT CCCAAAAACG GATTTTGGAA CTGGAATCTC ACCTGGCCAA GAAAGACCAC
    CTTCTTTTGG AACAGAAGAA ATATCTAGAG GATGTCAAAC TCCAGGCAAG AGGACAGCTG
    CAGGCCGCAG AGAGCAGGTA TGAGGCTCAG AAAAGGATAA CCCAGGTGTT TGAATTGGAG
    ATCTTAGATT TATATGGCAG GTTGGAGAAA GATGGCCTCC TGAAAAAACT TGAAGAAGAA
    AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA AGGCTTGACT GTTGTAATGA CGGGTGCTCA
    GATTCCATGG TAGGGCACAA TGAAGAGGCA TCTGGCCACA ACGGTGAGAC CAAGACCCCC
    AGGCCCAGCA GCGCCCGGGG CAGTAGTGGA AGCAGAGGTG GTGGAGGCAG CAGCAGCAGC
    AGCAGCGAGC TTTCTACCCC AGAGAAACCC CCACACCAGA GGGCAGGCCC ATTCAGCAGT
    CGGTGGGAGA CGACTATGGG AGAAGCGTCT GCCAGCATCC CCACCACTGT GGGCTCACTT
    CCCAGTTCAA AAAGCTTCCT GGGTATGAAG GCTCGAGAGT TATTTCGTAA TAAGAGCGAG
    AGCCAGTGTG ATGAGGACGG CATGACCAGT AGCCTTTCTG AGAGCCTAAA GACAGAACTG
    GGCAAAGACT TGGGTGTGGA AGCCAAGATT CCCCTGAACC TAGATGGCCC TCACCCGTCT
    CCCCCGACCC CGGACAGTGT TGGACAGCTA CATATCATGG ACTACAATGA GACTCATCAT
    GAACACAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu98884
    Clone ID Related Accession (Same CDS sequence) XM_017015099.1 , NM_001362177.1 , NM_001362177.2 , XM_017015100.1
    Accession Version XM_017015099.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3132bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product hamartin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGACACTG ACGTCGTTGT CCTCACAACA GGCGTCTTGG TGTTGATAAC CATGCTACCA 
    ATGATTCCAC AGTCTGGGAA ACAGCATCTT CTTGATTTCT TTGACATTTT TGGCCGTCTG
    TCATCATGGT GCCTGAAGAA ACCAGGCCAC GTGGCGGAAG TCTATCTCGT CCATCTCCAT
    GCCAGTGTGT ACGCACTCTT TCATCGCCTT TATGGAATGT ACCCTTGCAA CTTCGTCTCC
    TTTTTGCGTT CTCATTACAG TATGAAAGAA AACCTGGAGA CTTTTGAAGA AGTGGTCAAG
    CCAATGATGG AGCATGTGCG AATTCATCCG GAATTAGTGA CTGGATCCAA GGACCATGAA
    CTGGACCCTC GAAGGTGGAA GAGATTAGAA ACTCATGATG TTGTGATCGA GTGTGCCAAA
    ATCTCTCTGG ATCCCACAGA AGCCTCATAT GAAGATGGCT ATTCTGTGTC TCACCAAATC
    TCAGCCCGCT TTCCTCATCG TTCAGCCGAT GTCACCACCA GCCCTTATGC TGACACACAG
    AATAGCTATG GGTGTGCTAC TTCTACCCCT TACTCCACGT CTCGGCTGAT GTTGTTAAAT
    ATGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCATCGA CACGGCTGAT AACTGAACCA
    CCACAAGCTA CTCTTTGGAG CCCATCTATG GTTTGTGGTA TGACCACTCC TCCAACTTCT
    CCTGGAAATG TCCCACCTGA TCTGTCACAC CCTTACAGTA AAGTCTTTGG TACAACTGCA
    GGTGGAAAAG GAACTCCTCT GGGAACCCCA GCAACCTCTC CTCCTCCAGC CCCACTCTGT
    CATTCGGATG ACTACGTGCA CATTTCACTC CCCCAGGCCA CAGTCACACC CCCCAGGAAG
    GAAGAGAGAA TGGATTCTGC AAGACCATGT CTACACAGAC AACACCATCT TCTGAATGAC
    AGAGGATCAG AAGAGCCACC TGGCAGCAAA GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG
    TTTTTAGGTG ATCTGGCCTC TGAAGAAGAT AGTATTGAAA AAGATAAAGA AGAAGCTGCA
    ATATCTAGAG AACTTTCTGA GATCACCACA GCAGAGGCAG AGCCTGTGGT TCCTCGAGGA
    GGCTTTGACT CTCCCTTTTA CCGAGACAGT CTCCCAGGTT CTCAGCGGAA GACCCACTCG
    GCAGCCTCCA GTTCTCAGGG CGCCAGCGTG AACCCTGAGC CTTTACACTC CTCCCTGGAC
    AAGCTTGGGC CTGACACACC AAAGCAAGCC TTTACTCCCA TAGACCTGCC CTGCGGCAGT
    GCTGATGAAA GCCCTGCGGG AGACAGGGAA TGCCAGACTT CTTTGGAGAC CAGTATCTTC
    ACTCCCAGTC CTTGTAAAAT TCCACCTCCG ACGAGAGTGG GCTTTGGAAG CGGGCAGCCT
    CCCCCGTATG ATCATCTTTT TGAGGTGGCA TTGCCAAAGA CAGCCCATCA TTTTGTCATC
    AGGAAGACTG AGGAGCTGTT AAAGAAAGCA AAAGGAAACA CAGAGGAAGA TGGTGTGCCC
    TCTACCTCCC CAATGGAAGT GCTGGACAGA CTGATACAGC AGGGAGCAGA CGCGCACAGC
    AAGGAGCTGA ACAAGTTGCC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTTGGAGGC
    TCTCCTCCTT CAGATGAGAT CCGCACCCTC CGAGACCAGT TGCTTTTACT GCACAACCAG
    TTACTCTATG AGCGTTTTAA GAGGCAGCAG CATGCCCTCC GGAACAGGCG GCTCCTCCGC
    AAGGTGATCA AAGCAGCAGC TCTGGAGGAA CATAATGCTG CCATGAAAGA TCAGTTGAAG
    TTACAAGAGA AGGACATCCA GATGTGGAAG GTTAGTCTGC AGAAAGAACA AGCTAGATAC
    AATCAGCTCC AGGAGCAGCG TGACACTATG GTAACCAAGC TCCACAGCCA GATCAGACAG
    CTGCAGCATG ACCGAGAGGA ATTCTACAAC CAGAGCCAGG AATTACAGAC GAAGCTGGAG
    GACTGCAGGA ACATGATTGC GGAGCTGCGG ATAGAACTGA AGAAGGCCAA CAACAAGGTG
    TGTCACACTG AGCTGCTGCT CAGTCAGGTT TCCCAAAAGC TCTCAAACAG TGAGTCGGTC
    CAGCAGCAGA TGGAGTTCTT GAACAGGCAG CTGTTGGTTC TTGGGGAGGT CAACGAGCTC
    TATTTGGAAC AACTGCAGAA CAAGCACTCA GATACCACAA AGGAAGTAGA AATGATGAAA
    GCCGCCTATC GGAAAGAGCT AGAAAAAAAC AGAAGCCATG TTCTCCAGCA GACTCAGAGG
    CTTGATACCT CCCAAAAACG GATTTTGGAA CTGGAATCTC ACCTGGCCAA GAAAGACCAC
    CTTCTTTTGG AACAGAAGAA ATATCTAGAG GATGTCAAAC TCCAGGCAAG AGGACAGCTG
    CAGGCCGCAG AGAGCAGGTA TGAGGCTCAG AAAAGGATAA CCCAGGTGTT TGAATTGGAG
    ATCTTAGATT TATATGGCAG GTTGGAGAAA GATGGCCTCC TGAAAAAACT TGAAGAAGAA
    AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA AGGCTTGACT GTTGTAATGA CGGGTGCTCA
    GATTCCATGG TAGGGCACAA TGAAGAGGCA TCTGGCCACA ACGGTGAGAC CAAGACCCCC
    AGGCCCAGCA GCGCCCGGGG CAGTAGTGGA AGCAGAGGTG GTGGAGGCAG CAGCAGCAGC
    AGCAGCGAGC TTTCTACCCC AGAGAAACCC CCACACCAGA GGGCAGGCCC ATTCAGCAGT
    CGGTGGGAGA CGACTATGGG AGAAGCGTCT GCCAGCATCC CCACCACTGT GGGCTCACTT
    CCCAGTTCAA AAAGCTTCCT GGGTATGAAG GCTCGAGAGT TATTTCGTAA TAAGAGCGAG
    AGCCAGTGTG ATGAGGACGG CATGACCAGT AGCCTTTCTG AGAGCCTAAA GACAGAACTG
    GGCAAAGACT TGGGTGTGGA AGCCAAGATT CCCCTGAACC TAGATGGCCC TCACCCGTCT
    CCCCCGACCC CGGACAGTGT TGGACAGCTA CATATCATGG ACTACAATGA GACTCATCAT
    GAACACAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016870588.1
    CDS736..3867
    Translation

    Target ORF information:

    RefSeq Version XM_017015099.1
    Organism Homo sapiens(human)
    Definition Homo sapiens tuberous sclerosis 1 (TSC1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017015099.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGACACTG ACGTCGTTGT CCTCACAACA GGCGTCTTGG TGTTGATAAC CATGCTACCA 
    ATGATTCCAC AGTCTGGGAA ACAGCATCTT CTTGATTTCT TTGACATTTT TGGCCGTCTG
    TCATCATGGT GCCTGAAGAA ACCAGGCCAC GTGGCGGAAG TCTATCTCGT CCATCTCCAT
    GCCAGTGTGT ACGCACTCTT TCATCGCCTT TATGGAATGT ACCCTTGCAA CTTCGTCTCC
    TTTTTGCGTT CTCATTACAG TATGAAAGAA AACCTGGAGA CTTTTGAAGA AGTGGTCAAG
    CCAATGATGG AGCATGTGCG AATTCATCCG GAATTAGTGA CTGGATCCAA GGACCATGAA
    CTGGACCCTC GAAGGTGGAA GAGATTAGAA ACTCATGATG TTGTGATCGA GTGTGCCAAA
    ATCTCTCTGG ATCCCACAGA AGCCTCATAT GAAGATGGCT ATTCTGTGTC TCACCAAATC
    TCAGCCCGCT TTCCTCATCG TTCAGCCGAT GTCACCACCA GCCCTTATGC TGACACACAG
    AATAGCTATG GGTGTGCTAC TTCTACCCCT TACTCCACGT CTCGGCTGAT GTTGTTAAAT
    ATGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCATCGA CACGGCTGAT AACTGAACCA
    CCACAAGCTA CTCTTTGGAG CCCATCTATG GTTTGTGGTA TGACCACTCC TCCAACTTCT
    CCTGGAAATG TCCCACCTGA TCTGTCACAC CCTTACAGTA AAGTCTTTGG TACAACTGCA
    GGTGGAAAAG GAACTCCTCT GGGAACCCCA GCAACCTCTC CTCCTCCAGC CCCACTCTGT
    CATTCGGATG ACTACGTGCA CATTTCACTC CCCCAGGCCA CAGTCACACC CCCCAGGAAG
    GAAGAGAGAA TGGATTCTGC AAGACCATGT CTACACAGAC AACACCATCT TCTGAATGAC
    AGAGGATCAG AAGAGCCACC TGGCAGCAAA GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG
    TTTTTAGGTG ATCTGGCCTC TGAAGAAGAT AGTATTGAAA AAGATAAAGA AGAAGCTGCA
    ATATCTAGAG AACTTTCTGA GATCACCACA GCAGAGGCAG AGCCTGTGGT TCCTCGAGGA
    GGCTTTGACT CTCCCTTTTA CCGAGACAGT CTCCCAGGTT CTCAGCGGAA GACCCACTCG
    GCAGCCTCCA GTTCTCAGGG CGCCAGCGTG AACCCTGAGC CTTTACACTC CTCCCTGGAC
    AAGCTTGGGC CTGACACACC AAAGCAAGCC TTTACTCCCA TAGACCTGCC CTGCGGCAGT
    GCTGATGAAA GCCCTGCGGG AGACAGGGAA TGCCAGACTT CTTTGGAGAC CAGTATCTTC
    ACTCCCAGTC CTTGTAAAAT TCCACCTCCG ACGAGAGTGG GCTTTGGAAG CGGGCAGCCT
    CCCCCGTATG ATCATCTTTT TGAGGTGGCA TTGCCAAAGA CAGCCCATCA TTTTGTCATC
    AGGAAGACTG AGGAGCTGTT AAAGAAAGCA AAAGGAAACA CAGAGGAAGA TGGTGTGCCC
    TCTACCTCCC CAATGGAAGT GCTGGACAGA CTGATACAGC AGGGAGCAGA CGCGCACAGC
    AAGGAGCTGA ACAAGTTGCC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTTGGAGGC
    TCTCCTCCTT CAGATGAGAT CCGCACCCTC CGAGACCAGT TGCTTTTACT GCACAACCAG
    TTACTCTATG AGCGTTTTAA GAGGCAGCAG CATGCCCTCC GGAACAGGCG GCTCCTCCGC
    AAGGTGATCA AAGCAGCAGC TCTGGAGGAA CATAATGCTG CCATGAAAGA TCAGTTGAAG
    TTACAAGAGA AGGACATCCA GATGTGGAAG GTTAGTCTGC AGAAAGAACA AGCTAGATAC
    AATCAGCTCC AGGAGCAGCG TGACACTATG GTAACCAAGC TCCACAGCCA GATCAGACAG
    CTGCAGCATG ACCGAGAGGA ATTCTACAAC CAGAGCCAGG AATTACAGAC GAAGCTGGAG
    GACTGCAGGA ACATGATTGC GGAGCTGCGG ATAGAACTGA AGAAGGCCAA CAACAAGGTG
    TGTCACACTG AGCTGCTGCT CAGTCAGGTT TCCCAAAAGC TCTCAAACAG TGAGTCGGTC
    CAGCAGCAGA TGGAGTTCTT GAACAGGCAG CTGTTGGTTC TTGGGGAGGT CAACGAGCTC
    TATTTGGAAC AACTGCAGAA CAAGCACTCA GATACCACAA AGGAAGTAGA AATGATGAAA
    GCCGCCTATC GGAAAGAGCT AGAAAAAAAC AGAAGCCATG TTCTCCAGCA GACTCAGAGG
    CTTGATACCT CCCAAAAACG GATTTTGGAA CTGGAATCTC ACCTGGCCAA GAAAGACCAC
    CTTCTTTTGG AACAGAAGAA ATATCTAGAG GATGTCAAAC TCCAGGCAAG AGGACAGCTG
    CAGGCCGCAG AGAGCAGGTA TGAGGCTCAG AAAAGGATAA CCCAGGTGTT TGAATTGGAG
    ATCTTAGATT TATATGGCAG GTTGGAGAAA GATGGCCTCC TGAAAAAACT TGAAGAAGAA
    AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA AGGCTTGACT GTTGTAATGA CGGGTGCTCA
    GATTCCATGG TAGGGCACAA TGAAGAGGCA TCTGGCCACA ACGGTGAGAC CAAGACCCCC
    AGGCCCAGCA GCGCCCGGGG CAGTAGTGGA AGCAGAGGTG GTGGAGGCAG CAGCAGCAGC
    AGCAGCGAGC TTTCTACCCC AGAGAAACCC CCACACCAGA GGGCAGGCCC ATTCAGCAGT
    CGGTGGGAGA CGACTATGGG AGAAGCGTCT GCCAGCATCC CCACCACTGT GGGCTCACTT
    CCCAGTTCAA AAAGCTTCCT GGGTATGAAG GCTCGAGAGT TATTTCGTAA TAAGAGCGAG
    AGCCAGTGTG ATGAGGACGG CATGACCAGT AGCCTTTCTG AGAGCCTAAA GACAGAACTG
    GGCAAAGACT TGGGTGTGGA AGCCAAGATT CCCCTGAACC TAGATGGCCC TCACCCGTCT
    CCCCCGACCC CGGACAGTGT TGGACAGCTA CATATCATGG ACTACAATGA GACTCATCAT
    GAACACAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu98884
    Clone ID Related Accession (Same CDS sequence) XM_017015099.1 , NM_001362177.1 , NM_001362177.2 , XM_017015100.1
    Accession Version XM_017015100.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3132bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product hamartin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGACACTG ACGTCGTTGT CCTCACAACA GGCGTCTTGG TGTTGATAAC CATGCTACCA 
    ATGATTCCAC AGTCTGGGAA ACAGCATCTT CTTGATTTCT TTGACATTTT TGGCCGTCTG
    TCATCATGGT GCCTGAAGAA ACCAGGCCAC GTGGCGGAAG TCTATCTCGT CCATCTCCAT
    GCCAGTGTGT ACGCACTCTT TCATCGCCTT TATGGAATGT ACCCTTGCAA CTTCGTCTCC
    TTTTTGCGTT CTCATTACAG TATGAAAGAA AACCTGGAGA CTTTTGAAGA AGTGGTCAAG
    CCAATGATGG AGCATGTGCG AATTCATCCG GAATTAGTGA CTGGATCCAA GGACCATGAA
    CTGGACCCTC GAAGGTGGAA GAGATTAGAA ACTCATGATG TTGTGATCGA GTGTGCCAAA
    ATCTCTCTGG ATCCCACAGA AGCCTCATAT GAAGATGGCT ATTCTGTGTC TCACCAAATC
    TCAGCCCGCT TTCCTCATCG TTCAGCCGAT GTCACCACCA GCCCTTATGC TGACACACAG
    AATAGCTATG GGTGTGCTAC TTCTACCCCT TACTCCACGT CTCGGCTGAT GTTGTTAAAT
    ATGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCATCGA CACGGCTGAT AACTGAACCA
    CCACAAGCTA CTCTTTGGAG CCCATCTATG GTTTGTGGTA TGACCACTCC TCCAACTTCT
    CCTGGAAATG TCCCACCTGA TCTGTCACAC CCTTACAGTA AAGTCTTTGG TACAACTGCA
    GGTGGAAAAG GAACTCCTCT GGGAACCCCA GCAACCTCTC CTCCTCCAGC CCCACTCTGT
    CATTCGGATG ACTACGTGCA CATTTCACTC CCCCAGGCCA CAGTCACACC CCCCAGGAAG
    GAAGAGAGAA TGGATTCTGC AAGACCATGT CTACACAGAC AACACCATCT TCTGAATGAC
    AGAGGATCAG AAGAGCCACC TGGCAGCAAA GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG
    TTTTTAGGTG ATCTGGCCTC TGAAGAAGAT AGTATTGAAA AAGATAAAGA AGAAGCTGCA
    ATATCTAGAG AACTTTCTGA GATCACCACA GCAGAGGCAG AGCCTGTGGT TCCTCGAGGA
    GGCTTTGACT CTCCCTTTTA CCGAGACAGT CTCCCAGGTT CTCAGCGGAA GACCCACTCG
    GCAGCCTCCA GTTCTCAGGG CGCCAGCGTG AACCCTGAGC CTTTACACTC CTCCCTGGAC
    AAGCTTGGGC CTGACACACC AAAGCAAGCC TTTACTCCCA TAGACCTGCC CTGCGGCAGT
    GCTGATGAAA GCCCTGCGGG AGACAGGGAA TGCCAGACTT CTTTGGAGAC CAGTATCTTC
    ACTCCCAGTC CTTGTAAAAT TCCACCTCCG ACGAGAGTGG GCTTTGGAAG CGGGCAGCCT
    CCCCCGTATG ATCATCTTTT TGAGGTGGCA TTGCCAAAGA CAGCCCATCA TTTTGTCATC
    AGGAAGACTG AGGAGCTGTT AAAGAAAGCA AAAGGAAACA CAGAGGAAGA TGGTGTGCCC
    TCTACCTCCC CAATGGAAGT GCTGGACAGA CTGATACAGC AGGGAGCAGA CGCGCACAGC
    AAGGAGCTGA ACAAGTTGCC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTTGGAGGC
    TCTCCTCCTT CAGATGAGAT CCGCACCCTC CGAGACCAGT TGCTTTTACT GCACAACCAG
    TTACTCTATG AGCGTTTTAA GAGGCAGCAG CATGCCCTCC GGAACAGGCG GCTCCTCCGC
    AAGGTGATCA AAGCAGCAGC TCTGGAGGAA CATAATGCTG CCATGAAAGA TCAGTTGAAG
    TTACAAGAGA AGGACATCCA GATGTGGAAG GTTAGTCTGC AGAAAGAACA AGCTAGATAC
    AATCAGCTCC AGGAGCAGCG TGACACTATG GTAACCAAGC TCCACAGCCA GATCAGACAG
    CTGCAGCATG ACCGAGAGGA ATTCTACAAC CAGAGCCAGG AATTACAGAC GAAGCTGGAG
    GACTGCAGGA ACATGATTGC GGAGCTGCGG ATAGAACTGA AGAAGGCCAA CAACAAGGTG
    TGTCACACTG AGCTGCTGCT CAGTCAGGTT TCCCAAAAGC TCTCAAACAG TGAGTCGGTC
    CAGCAGCAGA TGGAGTTCTT GAACAGGCAG CTGTTGGTTC TTGGGGAGGT CAACGAGCTC
    TATTTGGAAC AACTGCAGAA CAAGCACTCA GATACCACAA AGGAAGTAGA AATGATGAAA
    GCCGCCTATC GGAAAGAGCT AGAAAAAAAC AGAAGCCATG TTCTCCAGCA GACTCAGAGG
    CTTGATACCT CCCAAAAACG GATTTTGGAA CTGGAATCTC ACCTGGCCAA GAAAGACCAC
    CTTCTTTTGG AACAGAAGAA ATATCTAGAG GATGTCAAAC TCCAGGCAAG AGGACAGCTG
    CAGGCCGCAG AGAGCAGGTA TGAGGCTCAG AAAAGGATAA CCCAGGTGTT TGAATTGGAG
    ATCTTAGATT TATATGGCAG GTTGGAGAAA GATGGCCTCC TGAAAAAACT TGAAGAAGAA
    AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA AGGCTTGACT GTTGTAATGA CGGGTGCTCA
    GATTCCATGG TAGGGCACAA TGAAGAGGCA TCTGGCCACA ACGGTGAGAC CAAGACCCCC
    AGGCCCAGCA GCGCCCGGGG CAGTAGTGGA AGCAGAGGTG GTGGAGGCAG CAGCAGCAGC
    AGCAGCGAGC TTTCTACCCC AGAGAAACCC CCACACCAGA GGGCAGGCCC ATTCAGCAGT
    CGGTGGGAGA CGACTATGGG AGAAGCGTCT GCCAGCATCC CCACCACTGT GGGCTCACTT
    CCCAGTTCAA AAAGCTTCCT GGGTATGAAG GCTCGAGAGT TATTTCGTAA TAAGAGCGAG
    AGCCAGTGTG ATGAGGACGG CATGACCAGT AGCCTTTCTG AGAGCCTAAA GACAGAACTG
    GGCAAAGACT TGGGTGTGGA AGCCAAGATT CCCCTGAACC TAGATGGCCC TCACCCGTCT
    CCCCCGACCC CGGACAGTGT TGGACAGCTA CATATCATGG ACTACAATGA GACTCATCAT
    GAACACAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016870589.1
    CDS707..3838
    Translation

    Target ORF information:

    RefSeq Version XM_017015100.1
    Organism Homo sapiens(human)
    Definition Homo sapiens TSC complex subunit 1 (TSC1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017015100.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGACACTG ACGTCGTTGT CCTCACAACA GGCGTCTTGG TGTTGATAAC CATGCTACCA 
    ATGATTCCAC AGTCTGGGAA ACAGCATCTT CTTGATTTCT TTGACATTTT TGGCCGTCTG
    TCATCATGGT GCCTGAAGAA ACCAGGCCAC GTGGCGGAAG TCTATCTCGT CCATCTCCAT
    GCCAGTGTGT ACGCACTCTT TCATCGCCTT TATGGAATGT ACCCTTGCAA CTTCGTCTCC
    TTTTTGCGTT CTCATTACAG TATGAAAGAA AACCTGGAGA CTTTTGAAGA AGTGGTCAAG
    CCAATGATGG AGCATGTGCG AATTCATCCG GAATTAGTGA CTGGATCCAA GGACCATGAA
    CTGGACCCTC GAAGGTGGAA GAGATTAGAA ACTCATGATG TTGTGATCGA GTGTGCCAAA
    ATCTCTCTGG ATCCCACAGA AGCCTCATAT GAAGATGGCT ATTCTGTGTC TCACCAAATC
    TCAGCCCGCT TTCCTCATCG TTCAGCCGAT GTCACCACCA GCCCTTATGC TGACACACAG
    AATAGCTATG GGTGTGCTAC TTCTACCCCT TACTCCACGT CTCGGCTGAT GTTGTTAAAT
    ATGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCATCGA CACGGCTGAT AACTGAACCA
    CCACAAGCTA CTCTTTGGAG CCCATCTATG GTTTGTGGTA TGACCACTCC TCCAACTTCT
    CCTGGAAATG TCCCACCTGA TCTGTCACAC CCTTACAGTA AAGTCTTTGG TACAACTGCA
    GGTGGAAAAG GAACTCCTCT GGGAACCCCA GCAACCTCTC CTCCTCCAGC CCCACTCTGT
    CATTCGGATG ACTACGTGCA CATTTCACTC CCCCAGGCCA CAGTCACACC CCCCAGGAAG
    GAAGAGAGAA TGGATTCTGC AAGACCATGT CTACACAGAC AACACCATCT TCTGAATGAC
    AGAGGATCAG AAGAGCCACC TGGCAGCAAA GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG
    TTTTTAGGTG ATCTGGCCTC TGAAGAAGAT AGTATTGAAA AAGATAAAGA AGAAGCTGCA
    ATATCTAGAG AACTTTCTGA GATCACCACA GCAGAGGCAG AGCCTGTGGT TCCTCGAGGA
    GGCTTTGACT CTCCCTTTTA CCGAGACAGT CTCCCAGGTT CTCAGCGGAA GACCCACTCG
    GCAGCCTCCA GTTCTCAGGG CGCCAGCGTG AACCCTGAGC CTTTACACTC CTCCCTGGAC
    AAGCTTGGGC CTGACACACC AAAGCAAGCC TTTACTCCCA TAGACCTGCC CTGCGGCAGT
    GCTGATGAAA GCCCTGCGGG AGACAGGGAA TGCCAGACTT CTTTGGAGAC CAGTATCTTC
    ACTCCCAGTC CTTGTAAAAT TCCACCTCCG ACGAGAGTGG GCTTTGGAAG CGGGCAGCCT
    CCCCCGTATG ATCATCTTTT TGAGGTGGCA TTGCCAAAGA CAGCCCATCA TTTTGTCATC
    AGGAAGACTG AGGAGCTGTT AAAGAAAGCA AAAGGAAACA CAGAGGAAGA TGGTGTGCCC
    TCTACCTCCC CAATGGAAGT GCTGGACAGA CTGATACAGC AGGGAGCAGA CGCGCACAGC
    AAGGAGCTGA ACAAGTTGCC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTTGGAGGC
    TCTCCTCCTT CAGATGAGAT CCGCACCCTC CGAGACCAGT TGCTTTTACT GCACAACCAG
    TTACTCTATG AGCGTTTTAA GAGGCAGCAG CATGCCCTCC GGAACAGGCG GCTCCTCCGC
    AAGGTGATCA AAGCAGCAGC TCTGGAGGAA CATAATGCTG CCATGAAAGA TCAGTTGAAG
    TTACAAGAGA AGGACATCCA GATGTGGAAG GTTAGTCTGC AGAAAGAACA AGCTAGATAC
    AATCAGCTCC AGGAGCAGCG TGACACTATG GTAACCAAGC TCCACAGCCA GATCAGACAG
    CTGCAGCATG ACCGAGAGGA ATTCTACAAC CAGAGCCAGG AATTACAGAC GAAGCTGGAG
    GACTGCAGGA ACATGATTGC GGAGCTGCGG ATAGAACTGA AGAAGGCCAA CAACAAGGTG
    TGTCACACTG AGCTGCTGCT CAGTCAGGTT TCCCAAAAGC TCTCAAACAG TGAGTCGGTC
    CAGCAGCAGA TGGAGTTCTT GAACAGGCAG CTGTTGGTTC TTGGGGAGGT CAACGAGCTC
    TATTTGGAAC AACTGCAGAA CAAGCACTCA GATACCACAA AGGAAGTAGA AATGATGAAA
    GCCGCCTATC GGAAAGAGCT AGAAAAAAAC AGAAGCCATG TTCTCCAGCA GACTCAGAGG
    CTTGATACCT CCCAAAAACG GATTTTGGAA CTGGAATCTC ACCTGGCCAA GAAAGACCAC
    CTTCTTTTGG AACAGAAGAA ATATCTAGAG GATGTCAAAC TCCAGGCAAG AGGACAGCTG
    CAGGCCGCAG AGAGCAGGTA TGAGGCTCAG AAAAGGATAA CCCAGGTGTT TGAATTGGAG
    ATCTTAGATT TATATGGCAG GTTGGAGAAA GATGGCCTCC TGAAAAAACT TGAAGAAGAA
    AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA AGGCTTGACT GTTGTAATGA CGGGTGCTCA
    GATTCCATGG TAGGGCACAA TGAAGAGGCA TCTGGCCACA ACGGTGAGAC CAAGACCCCC
    AGGCCCAGCA GCGCCCGGGG CAGTAGTGGA AGCAGAGGTG GTGGAGGCAG CAGCAGCAGC
    AGCAGCGAGC TTTCTACCCC AGAGAAACCC CCACACCAGA GGGCAGGCCC ATTCAGCAGT
    CGGTGGGAGA CGACTATGGG AGAAGCGTCT GCCAGCATCC CCACCACTGT GGGCTCACTT
    CCCAGTTCAA AAAGCTTCCT GGGTATGAAG GCTCGAGAGT TATTTCGTAA TAAGAGCGAG
    AGCCAGTGTG ATGAGGACGG CATGACCAGT AGCCTTTCTG AGAGCCTAAA GACAGAACTG
    GGCAAAGACT TGGGTGTGGA AGCCAAGATT CCCCTGAACC TAGATGGCCC TCACCCGTCT
    CCCCCGACCC CGGACAGTGT TGGACAGCTA CATATCATGG ACTACAATGA GACTCATCAT
    GAACACAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu98885
    Clone ID Related Accession (Same CDS sequence) XM_017015101.1
    Accession Version XM_017015101.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3129bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product hamartin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGACACTG ACGTCGTTGT CCTCACAACA GGCGTCTTGG TGTTGATAAC CATGCTACCA 
    ATGATTCCAC AGTCTGGGAA ACAGCATCTT CTTGATTTCT TTGACATTTT TGGCCGTCTG
    TCATCATGGT GCCTGAAGAA ACCAGGCCAC GTGGCGGAAG TCTATCTCGT CCATCTCCAT
    GCCAGTGTGT ACGCACTCTT TCATCGCCTT TATGGAATGT ACCCTTGCAA CTTCGTCTCC
    TTTTTGCGTT CTCATTACAG TATGAAAGAA AACCTGGAGA CTTTTGAAGA AGTGGTCAAG
    CCAATGATGG AGCATGTGCG AATTCATCCG GAATTAGTGA CTGGATCCAA GGACCATGAA
    CTGGACCCTC GAAGGTGGAA GAGATTAGAA ACTCATGATG TTGTGATCGA GTGTGCCAAA
    ATCTCTCTGG ATCCCACAGA AGCCTCATAT GAAGATGGCT ATTCTGTGTC TCACCAAATC
    TCAGCCCGCT TTCCTCATCG TTCAGCCGAT GTCACCACCA GCCCTTATGC TGACACACAG
    AATAGCTATG GGTGTGCTAC TTCTACCCCT TACTCCACGT CTCGGCTGAT GTTGTTAAAT
    ATGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCATCGA CACGGCTGAT AACTGAACCA
    CCACAAGCTA CTCTTTGGAG CCCATCTATG GTTTGTGGTA TGACCACTCC TCCAACTTCT
    CCTGGAAATG TCCCACCTGA TCTGTCACAC CCTTACAGTA AAGTCTTTGG TACAACTGGT
    GGAAAAGGAA CTCCTCTGGG AACCCCAGCA ACCTCTCCTC CTCCAGCCCC ACTCTGTCAT
    TCGGATGACT ACGTGCACAT TTCACTCCCC CAGGCCACAG TCACACCCCC CAGGAAGGAA
    GAGAGAATGG ATTCTGCAAG ACCATGTCTA CACAGACAAC ACCATCTTCT GAATGACAGA
    GGATCAGAAG AGCCACCTGG CAGCAAAGGT TCTGTCACTC TAAGTGATCT TCCAGGGTTT
    TTAGGTGATC TGGCCTCTGA AGAAGATAGT ATTGAAAAAG ATAAAGAAGA AGCTGCAATA
    TCTAGAGAAC TTTCTGAGAT CACCACAGCA GAGGCAGAGC CTGTGGTTCC TCGAGGAGGC
    TTTGACTCTC CCTTTTACCG AGACAGTCTC CCAGGTTCTC AGCGGAAGAC CCACTCGGCA
    GCCTCCAGTT CTCAGGGCGC CAGCGTGAAC CCTGAGCCTT TACACTCCTC CCTGGACAAG
    CTTGGGCCTG ACACACCAAA GCAAGCCTTT ACTCCCATAG ACCTGCCCTG CGGCAGTGCT
    GATGAAAGCC CTGCGGGAGA CAGGGAATGC CAGACTTCTT TGGAGACCAG TATCTTCACT
    CCCAGTCCTT GTAAAATTCC ACCTCCGACG AGAGTGGGCT TTGGAAGCGG GCAGCCTCCC
    CCGTATGATC ATCTTTTTGA GGTGGCATTG CCAAAGACAG CCCATCATTT TGTCATCAGG
    AAGACTGAGG AGCTGTTAAA GAAAGCAAAA GGAAACACAG AGGAAGATGG TGTGCCCTCT
    ACCTCCCCAA TGGAAGTGCT GGACAGACTG ATACAGCAGG GAGCAGACGC GCACAGCAAG
    GAGCTGAACA AGTTGCCTTT ACCCAGCAAG TCTGTCGACT GGACCCACTT TGGAGGCTCT
    CCTCCTTCAG ATGAGATCCG CACCCTCCGA GACCAGTTGC TTTTACTGCA CAACCAGTTA
    CTCTATGAGC GTTTTAAGAG GCAGCAGCAT GCCCTCCGGA ACAGGCGGCT CCTCCGCAAG
    GTGATCAAAG CAGCAGCTCT GGAGGAACAT AATGCTGCCA TGAAAGATCA GTTGAAGTTA
    CAAGAGAAGG ACATCCAGAT GTGGAAGGTT AGTCTGCAGA AAGAACAAGC TAGATACAAT
    CAGCTCCAGG AGCAGCGTGA CACTATGGTA ACCAAGCTCC ACAGCCAGAT CAGACAGCTG
    CAGCATGACC GAGAGGAATT CTACAACCAG AGCCAGGAAT TACAGACGAA GCTGGAGGAC
    TGCAGGAACA TGATTGCGGA GCTGCGGATA GAACTGAAGA AGGCCAACAA CAAGGTGTGT
    CACACTGAGC TGCTGCTCAG TCAGGTTTCC CAAAAGCTCT CAAACAGTGA GTCGGTCCAG
    CAGCAGATGG AGTTCTTGAA CAGGCAGCTG TTGGTTCTTG GGGAGGTCAA CGAGCTCTAT
    TTGGAACAAC TGCAGAACAA GCACTCAGAT ACCACAAAGG AAGTAGAAAT GATGAAAGCC
    GCCTATCGGA AAGAGCTAGA AAAAAACAGA AGCCATGTTC TCCAGCAGAC TCAGAGGCTT
    GATACCTCCC AAAAACGGAT TTTGGAACTG GAATCTCACC TGGCCAAGAA AGACCACCTT
    CTTTTGGAAC AGAAGAAATA TCTAGAGGAT GTCAAACTCC AGGCAAGAGG ACAGCTGCAG
    GCCGCAGAGA GCAGGTATGA GGCTCAGAAA AGGATAACCC AGGTGTTTGA ATTGGAGATC
    TTAGATTTAT ATGGCAGGTT GGAGAAAGAT GGCCTCCTGA AAAAACTTGA AGAAGAAAAA
    GCAGAAGCAG CTGAAGCAGC AGAAGAAAGG CTTGACTGTT GTAATGACGG GTGCTCAGAT
    TCCATGGTAG GGCACAATGA AGAGGCATCT GGCCACAACG GTGAGACCAA GACCCCCAGG
    CCCAGCAGCG CCCGGGGCAG TAGTGGAAGC AGAGGTGGTG GAGGCAGCAG CAGCAGCAGC
    AGCGAGCTTT CTACCCCAGA GAAACCCCCA CACCAGAGGG CAGGCCCATT CAGCAGTCGG
    TGGGAGACGA CTATGGGAGA AGCGTCTGCC AGCATCCCCA CCACTGTGGG CTCACTTCCC
    AGTTCAAAAA GCTTCCTGGG TATGAAGGCT CGAGAGTTAT TTCGTAATAA GAGCGAGAGC
    CAGTGTGATG AGGACGGCAT GACCAGTAGC CTTTCTGAGA GCCTAAAGAC AGAACTGGGC
    AAAGACTTGG GTGTGGAAGC CAAGATTCCC CTGAACCTAG ATGGCCCTCA CCCGTCTCCC
    CCGACCCCGG ACAGTGTTGG ACAGCTACAT ATCATGGACT ACAATGAGAC TCATCATGAA
    CACAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016870590.1
    CDS737..3865
    Translation

    Target ORF information:

    RefSeq Version XM_017015101.1
    Organism Homo sapiens(human)
    Definition Homo sapiens TSC complex subunit 1 (TSC1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017015101.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGACACTG ACGTCGTTGT CCTCACAACA GGCGTCTTGG TGTTGATAAC CATGCTACCA 
    ATGATTCCAC AGTCTGGGAA ACAGCATCTT CTTGATTTCT TTGACATTTT TGGCCGTCTG
    TCATCATGGT GCCTGAAGAA ACCAGGCCAC GTGGCGGAAG TCTATCTCGT CCATCTCCAT
    GCCAGTGTGT ACGCACTCTT TCATCGCCTT TATGGAATGT ACCCTTGCAA CTTCGTCTCC
    TTTTTGCGTT CTCATTACAG TATGAAAGAA AACCTGGAGA CTTTTGAAGA AGTGGTCAAG
    CCAATGATGG AGCATGTGCG AATTCATCCG GAATTAGTGA CTGGATCCAA GGACCATGAA
    CTGGACCCTC GAAGGTGGAA GAGATTAGAA ACTCATGATG TTGTGATCGA GTGTGCCAAA
    ATCTCTCTGG ATCCCACAGA AGCCTCATAT GAAGATGGCT ATTCTGTGTC TCACCAAATC
    TCAGCCCGCT TTCCTCATCG TTCAGCCGAT GTCACCACCA GCCCTTATGC TGACACACAG
    AATAGCTATG GGTGTGCTAC TTCTACCCCT TACTCCACGT CTCGGCTGAT GTTGTTAAAT
    ATGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCATCGA CACGGCTGAT AACTGAACCA
    CCACAAGCTA CTCTTTGGAG CCCATCTATG GTTTGTGGTA TGACCACTCC TCCAACTTCT
    CCTGGAAATG TCCCACCTGA TCTGTCACAC CCTTACAGTA AAGTCTTTGG TACAACTGGT
    GGAAAAGGAA CTCCTCTGGG AACCCCAGCA ACCTCTCCTC CTCCAGCCCC ACTCTGTCAT
    TCGGATGACT ACGTGCACAT TTCACTCCCC CAGGCCACAG TCACACCCCC CAGGAAGGAA
    GAGAGAATGG ATTCTGCAAG ACCATGTCTA CACAGACAAC ACCATCTTCT GAATGACAGA
    GGATCAGAAG AGCCACCTGG CAGCAAAGGT TCTGTCACTC TAAGTGATCT TCCAGGGTTT
    TTAGGTGATC TGGCCTCTGA AGAAGATAGT ATTGAAAAAG ATAAAGAAGA AGCTGCAATA
    TCTAGAGAAC TTTCTGAGAT CACCACAGCA GAGGCAGAGC CTGTGGTTCC TCGAGGAGGC
    TTTGACTCTC CCTTTTACCG AGACAGTCTC CCAGGTTCTC AGCGGAAGAC CCACTCGGCA
    GCCTCCAGTT CTCAGGGCGC CAGCGTGAAC CCTGAGCCTT TACACTCCTC CCTGGACAAG
    CTTGGGCCTG ACACACCAAA GCAAGCCTTT ACTCCCATAG ACCTGCCCTG CGGCAGTGCT
    GATGAAAGCC CTGCGGGAGA CAGGGAATGC CAGACTTCTT TGGAGACCAG TATCTTCACT
    CCCAGTCCTT GTAAAATTCC ACCTCCGACG AGAGTGGGCT TTGGAAGCGG GCAGCCTCCC
    CCGTATGATC ATCTTTTTGA GGTGGCATTG CCAAAGACAG CCCATCATTT TGTCATCAGG
    AAGACTGAGG AGCTGTTAAA GAAAGCAAAA GGAAACACAG AGGAAGATGG TGTGCCCTCT
    ACCTCCCCAA TGGAAGTGCT GGACAGACTG ATACAGCAGG GAGCAGACGC GCACAGCAAG
    GAGCTGAACA AGTTGCCTTT ACCCAGCAAG TCTGTCGACT GGACCCACTT TGGAGGCTCT
    CCTCCTTCAG ATGAGATCCG CACCCTCCGA GACCAGTTGC TTTTACTGCA CAACCAGTTA
    CTCTATGAGC GTTTTAAGAG GCAGCAGCAT GCCCTCCGGA ACAGGCGGCT CCTCCGCAAG
    GTGATCAAAG CAGCAGCTCT GGAGGAACAT AATGCTGCCA TGAAAGATCA GTTGAAGTTA
    CAAGAGAAGG ACATCCAGAT GTGGAAGGTT AGTCTGCAGA AAGAACAAGC TAGATACAAT
    CAGCTCCAGG AGCAGCGTGA CACTATGGTA ACCAAGCTCC ACAGCCAGAT CAGACAGCTG
    CAGCATGACC GAGAGGAATT CTACAACCAG AGCCAGGAAT TACAGACGAA GCTGGAGGAC
    TGCAGGAACA TGATTGCGGA GCTGCGGATA GAACTGAAGA AGGCCAACAA CAAGGTGTGT
    CACACTGAGC TGCTGCTCAG TCAGGTTTCC CAAAAGCTCT CAAACAGTGA GTCGGTCCAG
    CAGCAGATGG AGTTCTTGAA CAGGCAGCTG TTGGTTCTTG GGGAGGTCAA CGAGCTCTAT
    TTGGAACAAC TGCAGAACAA GCACTCAGAT ACCACAAAGG AAGTAGAAAT GATGAAAGCC
    GCCTATCGGA AAGAGCTAGA AAAAAACAGA AGCCATGTTC TCCAGCAGAC TCAGAGGCTT
    GATACCTCCC AAAAACGGAT TTTGGAACTG GAATCTCACC TGGCCAAGAA AGACCACCTT
    CTTTTGGAAC AGAAGAAATA TCTAGAGGAT GTCAAACTCC AGGCAAGAGG ACAGCTGCAG
    GCCGCAGAGA GCAGGTATGA GGCTCAGAAA AGGATAACCC AGGTGTTTGA ATTGGAGATC
    TTAGATTTAT ATGGCAGGTT GGAGAAAGAT GGCCTCCTGA AAAAACTTGA AGAAGAAAAA
    GCAGAAGCAG CTGAAGCAGC AGAAGAAAGG CTTGACTGTT GTAATGACGG GTGCTCAGAT
    TCCATGGTAG GGCACAATGA AGAGGCATCT GGCCACAACG GTGAGACCAA GACCCCCAGG
    CCCAGCAGCG CCCGGGGCAG TAGTGGAAGC AGAGGTGGTG GAGGCAGCAG CAGCAGCAGC
    AGCGAGCTTT CTACCCCAGA GAAACCCCCA CACCAGAGGG CAGGCCCATT CAGCAGTCGG
    TGGGAGACGA CTATGGGAGA AGCGTCTGCC AGCATCCCCA CCACTGTGGG CTCACTTCCC
    AGTTCAAAAA GCTTCCTGGG TATGAAGGCT CGAGAGTTAT TTCGTAATAA GAGCGAGAGC
    CAGTGTGATG AGGACGGCAT GACCAGTAGC CTTTCTGAGA GCCTAAAGAC AGAACTGGGC
    AAAGACTTGG GTGTGGAAGC CAAGATTCCC CTGAACCTAG ATGGCCCTCA CCCGTCTCCC
    CCGACCCCGG ACAGTGTTGG ACAGCTACAT ATCATGGACT ACAATGAGAC TCATCATGAA
    CACAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17591
    Clone ID Related Accession (Same CDS sequence) NM_001162427.1 , NM_001162427.2
    Accession Version NM_001162427.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3342bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product hamartin isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI463204.1, AK303030.1, AF013168.1, D87683.1 and N63914.1. Transcript Variant: This variant (4) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK303030.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
    GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
    CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
    CTGACCACCT TGCAAGAGCC ACATGACAAG ATGGACACTG ACGTCGTTGT CCTCACAACA
    GGCGTCTTGG TGTTGATAAC CATGCTACCA ATGATTCCAC AGTCTGGGAA ACAGCATCTT
    CTTGATTTCT TTGACATTTT TGGCCGTCTG TCATCATGGT GCCTGAAGAA ACCAGGCCAC
    GTGGCGGAAG TCTATCTCGT CCATCTCCAT GCCAGTGTGT ACGCACTCTT TCATCGCCTT
    TATGGAATGT ACCCTTGCAA CTTCGTCTCC TTTTTGCGTT CTCATTACAG TATGAAAGAA
    AACCTGGAGA CTTTTGAAGA AGTGGTCAAG CCAATGATGG AGCATGTGCG AATTCATCCG
    GAATTAGTGA CTGGATCCAA GGACCATGAA CTGGACCCTC GAAGGTGGAA GAGATTAGAA
    ACTCATGATG TTGTGATCGA GTGTGCCAAA ATCTCTCTGG ATCCCACAGA AGCCTCATAT
    GAAGATGGCT ATTCTGTGTC TCACCAAATC TCAGCCCGCT TTCCTCATCG TTCAGCCGAT
    GTCACCACCA GCCCTTATGC TGACACACAG AATAGCTATG GGTGTGCTAC TTCTACCCCT
    TACTCCACGT CTCGGCTGAT GTTGTTAAAT ATGCCAGGGC AGCTACCTCA GACTCTGAGT
    TCCCCATCGA CACGGCTGAT AACTGAACCA CCACAAGCTA CTCTTTGGAG CCCATCTATG
    GTTTGTGGTA TGACCACTCC TCCAACTTCT CCTGGAAATG TCCCACCTGA TCTGTCACAC
    CCTTACAGTA AAGTCTTTGG TACAACTGCA GGTGGAAAAG GAACTCCTCT GGGAACCCCA
    GCAACCTCTC CTCCTCCAGC CCCACTCTGT CATTCGGATG ACTACGTGCA CATTTCACTC
    CCCCAGGCCA CAGTCACACC CCCCAGGAAG GAAGAGAGAA TGGATTCTGC AAGACCATGT
    CTACACAGAC AACACCATCT TCTGAATGAC AGAGGATCAG AAGAGCCACC TGGCAGCAAA
    GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG TTTTTAGGTG ATCTGGCCTC TGAAGAAGAT
    AGTATTGAAA AAGATAAAGA AGAAGCTGCA ATATCTAGAG AACTTTCTGA GATCACCACA
    GCAGAGGCAG AGCCTGTGGT TCCTCGAGGA GGCTTTGACT CTCCCTTTTA CCGAGACAGT
    CTCCCAGGTT CTCAGCGGAA GACCCACTCG GCAGCCTCCA GTTCTCAGGG CGCCAGCGTG
    AACCCTGAGC CTTTACACTC CTCCCTGGAC AAGCTTGGGC CTGACACACC AAAGCAAGCC
    TTTACTCCCA TAGACCTGCC CTGCGGCAGT GCTGATGAAA GCCCTGCGGG AGACAGGGAA
    TGCCAGACTT CTTTGGAGAC CAGTATCTTC ACTCCCAGTC CTTGTAAAAT TCCACCTCCG
    ACGAGAGTGG GCTTTGGAAG CGGGCAGCCT CCCCCGTATG ATCATCTTTT TGAGGTGGCA
    TTGCCAAAGA CAGCCCATCA TTTTGTCATC AGGAAGACTG AGGAGCTGTT AAAGAAAGCA
    AAAGGAAACA CAGAGGAAGA TGGTGTGCCC TCTACCTCCC CAATGGAAGT GCTGGACAGA
    CTGATACAGC AGGGAGCAGA CGCGCACAGC AAGGAGCTGA ACAAGTTGCC TTTACCCAGC
    AAGTCTGTCG ACTGGACCCA CTTTGGAGGC TCTCCTCCTT CAGATGAGAT CCGCACCCTC
    CGAGACCAGT TGCTTTTACT GCACAACCAG TTACTCTATG AGCGTTTTAA GAGGCAGCAG
    CATGCCCTCC GGAACAGGCG GCTCCTCCGC AAGGTGATCA AAGCAGCAGC TCTGGAGGAA
    CATAATGCTG CCATGAAAGA TCAGTTGAAG TTACAAGAGA AGGACATCCA GATGTGGAAG
    GTTAGTCTGC AGAAAGAACA AGCTAGATAC AATCAGCTCC AGGAGCAGCG TGACACTATG
    GTAACCAAGC TCCACAGCCA GATCAGACAG CTGCAGCATG ACCGAGAGGA ATTCTACAAC
    CAGAGCCAGG AATTACAGAC GAAGCTGGAG GACTGCAGGA ACATGATTGC GGAGCTGCGG
    ATAGAACTGA AGAAGGCCAA CAACAAGGTG TGTCACACTG AGCTGCTGCT CAGTCAGGTT
    TCCCAAAAGC TCTCAAACAG TGAGTCGGTC CAGCAGCAGA TGGAGTTCTT GAACAGGCAG
    CTGTTGGTTC TTGGGGAGGT CAACGAGCTC TATTTGGAAC AACTGCAGAA CAAGCACTCA
    GATACCACAA AGGAAGTAGA AATGATGAAA GCCGCCTATC GGAAAGAGCT AGAAAAAAAC
    AGAAGCCATG TTCTCCAGCA GACTCAGAGG CTTGATACCT CCCAAAAACG GATTTTGGAA
    CTGGAATCTC ACCTGGCCAA GAAAGACCAC CTTCTTTTGG AACAGAAGAA ATATCTAGAG
    GATGTCAAAC TCCAGGCAAG AGGACAGCTG CAGGCCGCAG AGAGCAGGTA TGAGGCTCAG
    AAAAGGATAA CCCAGGTGTT TGAATTGGAG ATCTTAGATT TATATGGCAG GTTGGAGAAA
    GATGGCCTCC TGAAAAAACT TGAAGAAGAA AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA
    AGGCTTGACT GTTGTAATGA CGGGTGCTCA GATTCCATGG TAGGGCACAA TGAAGAGGCA
    TCTGGCCACA ACGGTGAGAC CAAGACCCCC AGGCCCAGCA GCGCCCGGGG CAGTAGTGGA
    AGCAGAGGTG GTGGAGGCAG CAGCAGCAGC AGCAGCGAGC TTTCTACCCC AGAGAAACCC
    CCACACCAGA GGGCAGGCCC ATTCAGCAGT CGGTGGGAGA CGACTATGGG AGAAGCGTCT
    GCCAGCATCC CCACCACTGT GGGCTCACTT CCCAGTTCAA AAAGCTTCCT GGGTATGAAG
    GCTCGAGAGT TATTTCGTAA TAAGAGCGAG AGCCAGTGTG ATGAGGACGG CATGACCAGT
    AGCCTTTCTG AGAGCCTAAA GACAGAACTG GGCAAAGACT TGGGTGTGGA AGCCAAGATT
    CCCCTGAACC TAGATGGCCC TCACCCGTCT CCCCCGACCC CGGACAGTGT TGGACAGCTA
    CATATCATGG ACTACAATGA GACTCATCAT GAACACAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001155899.1
    CDS235..3576
    Translation

    Target ORF information:

    RefSeq Version NM_001162427.1
    Organism Homo sapiens(human)
    Definition Homo sapiens TSC complex subunit 1 (TSC1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001162427.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
    GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
    CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
    CTGACCACCT TGCAAGAGCC ACATGACAAG ATGGACACTG ACGTCGTTGT CCTCACAACA
    GGCGTCTTGG TGTTGATAAC CATGCTACCA ATGATTCCAC AGTCTGGGAA ACAGCATCTT
    CTTGATTTCT TTGACATTTT TGGCCGTCTG TCATCATGGT GCCTGAAGAA ACCAGGCCAC
    GTGGCGGAAG TCTATCTCGT CCATCTCCAT GCCAGTGTGT ACGCACTCTT TCATCGCCTT
    TATGGAATGT ACCCTTGCAA CTTCGTCTCC TTTTTGCGTT CTCATTACAG TATGAAAGAA
    AACCTGGAGA CTTTTGAAGA AGTGGTCAAG CCAATGATGG AGCATGTGCG AATTCATCCG
    GAATTAGTGA CTGGATCCAA GGACCATGAA CTGGACCCTC GAAGGTGGAA GAGATTAGAA
    ACTCATGATG TTGTGATCGA GTGTGCCAAA ATCTCTCTGG ATCCCACAGA AGCCTCATAT
    GAAGATGGCT ATTCTGTGTC TCACCAAATC TCAGCCCGCT TTCCTCATCG TTCAGCCGAT
    GTCACCACCA GCCCTTATGC TGACACACAG AATAGCTATG GGTGTGCTAC TTCTACCCCT
    TACTCCACGT CTCGGCTGAT GTTGTTAAAT ATGCCAGGGC AGCTACCTCA GACTCTGAGT
    TCCCCATCGA CACGGCTGAT AACTGAACCA CCACAAGCTA CTCTTTGGAG CCCATCTATG
    GTTTGTGGTA TGACCACTCC TCCAACTTCT CCTGGAAATG TCCCACCTGA TCTGTCACAC
    CCTTACAGTA AAGTCTTTGG TACAACTGCA GGTGGAAAAG GAACTCCTCT GGGAACCCCA
    GCAACCTCTC CTCCTCCAGC CCCACTCTGT CATTCGGATG ACTACGTGCA CATTTCACTC
    CCCCAGGCCA CAGTCACACC CCCCAGGAAG GAAGAGAGAA TGGATTCTGC AAGACCATGT
    CTACACAGAC AACACCATCT TCTGAATGAC AGAGGATCAG AAGAGCCACC TGGCAGCAAA
    GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG TTTTTAGGTG ATCTGGCCTC TGAAGAAGAT
    AGTATTGAAA AAGATAAAGA AGAAGCTGCA ATATCTAGAG AACTTTCTGA GATCACCACA
    GCAGAGGCAG AGCCTGTGGT TCCTCGAGGA GGCTTTGACT CTCCCTTTTA CCGAGACAGT
    CTCCCAGGTT CTCAGCGGAA GACCCACTCG GCAGCCTCCA GTTCTCAGGG CGCCAGCGTG
    AACCCTGAGC CTTTACACTC CTCCCTGGAC AAGCTTGGGC CTGACACACC AAAGCAAGCC
    TTTACTCCCA TAGACCTGCC CTGCGGCAGT GCTGATGAAA GCCCTGCGGG AGACAGGGAA
    TGCCAGACTT CTTTGGAGAC CAGTATCTTC ACTCCCAGTC CTTGTAAAAT TCCACCTCCG
    ACGAGAGTGG GCTTTGGAAG CGGGCAGCCT CCCCCGTATG ATCATCTTTT TGAGGTGGCA
    TTGCCAAAGA CAGCCCATCA TTTTGTCATC AGGAAGACTG AGGAGCTGTT AAAGAAAGCA
    AAAGGAAACA CAGAGGAAGA TGGTGTGCCC TCTACCTCCC CAATGGAAGT GCTGGACAGA
    CTGATACAGC AGGGAGCAGA CGCGCACAGC AAGGAGCTGA ACAAGTTGCC TTTACCCAGC
    AAGTCTGTCG ACTGGACCCA CTTTGGAGGC TCTCCTCCTT CAGATGAGAT CCGCACCCTC
    CGAGACCAGT TGCTTTTACT GCACAACCAG TTACTCTATG AGCGTTTTAA GAGGCAGCAG
    CATGCCCTCC GGAACAGGCG GCTCCTCCGC AAGGTGATCA AAGCAGCAGC TCTGGAGGAA
    CATAATGCTG CCATGAAAGA TCAGTTGAAG TTACAAGAGA AGGACATCCA GATGTGGAAG
    GTTAGTCTGC AGAAAGAACA AGCTAGATAC AATCAGCTCC AGGAGCAGCG TGACACTATG
    GTAACCAAGC TCCACAGCCA GATCAGACAG CTGCAGCATG ACCGAGAGGA ATTCTACAAC
    CAGAGCCAGG AATTACAGAC GAAGCTGGAG GACTGCAGGA ACATGATTGC GGAGCTGCGG
    ATAGAACTGA AGAAGGCCAA CAACAAGGTG TGTCACACTG AGCTGCTGCT CAGTCAGGTT
    TCCCAAAAGC TCTCAAACAG TGAGTCGGTC CAGCAGCAGA TGGAGTTCTT GAACAGGCAG
    CTGTTGGTTC TTGGGGAGGT CAACGAGCTC TATTTGGAAC AACTGCAGAA CAAGCACTCA
    GATACCACAA AGGAAGTAGA AATGATGAAA GCCGCCTATC GGAAAGAGCT AGAAAAAAAC
    AGAAGCCATG TTCTCCAGCA GACTCAGAGG CTTGATACCT CCCAAAAACG GATTTTGGAA
    CTGGAATCTC ACCTGGCCAA GAAAGACCAC CTTCTTTTGG AACAGAAGAA ATATCTAGAG
    GATGTCAAAC TCCAGGCAAG AGGACAGCTG CAGGCCGCAG AGAGCAGGTA TGAGGCTCAG
    AAAAGGATAA CCCAGGTGTT TGAATTGGAG ATCTTAGATT TATATGGCAG GTTGGAGAAA
    GATGGCCTCC TGAAAAAACT TGAAGAAGAA AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA
    AGGCTTGACT GTTGTAATGA CGGGTGCTCA GATTCCATGG TAGGGCACAA TGAAGAGGCA
    TCTGGCCACA ACGGTGAGAC CAAGACCCCC AGGCCCAGCA GCGCCCGGGG CAGTAGTGGA
    AGCAGAGGTG GTGGAGGCAG CAGCAGCAGC AGCAGCGAGC TTTCTACCCC AGAGAAACCC
    CCACACCAGA GGGCAGGCCC ATTCAGCAGT CGGTGGGAGA CGACTATGGG AGAAGCGTCT
    GCCAGCATCC CCACCACTGT GGGCTCACTT CCCAGTTCAA AAAGCTTCCT GGGTATGAAG
    GCTCGAGAGT TATTTCGTAA TAAGAGCGAG AGCCAGTGTG ATGAGGACGG CATGACCAGT
    AGCCTTTCTG AGAGCCTAAA GACAGAACTG GGCAAAGACT TGGGTGTGGA AGCCAAGATT
    CCCCTGAACC TAGATGGCCC TCACCCGTCT CCCCCGACCC CGGACAGTGT TGGACAGCTA
    CATATCATGG ACTACAATGA GACTCATCAT GAACACAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17591
    Clone ID Related Accession (Same CDS sequence) NM_001162427.1 , NM_001162427.2
    Accession Version NM_001162427.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3342bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-25
    Organism Homo sapiens(human)
    Product hamartin isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK303030.1, AF013168.1, D87683.1 and N63914.1. On Jun 2, 2019 this sequence version replaced NM_001162427.1. Transcript Variant: This variant (4) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK303030.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
    GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
    CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
    CTGACCACCT TGCAAGAGCC ACATGACAAG ATGGACACTG ACGTCGTTGT CCTCACAACA
    GGCGTCTTGG TGTTGATAAC CATGCTACCA ATGATTCCAC AGTCTGGGAA ACAGCATCTT
    CTTGATTTCT TTGACATTTT TGGCCGTCTG TCATCATGGT GCCTGAAGAA ACCAGGCCAC
    GTGGCGGAAG TCTATCTCGT CCATCTCCAT GCCAGTGTGT ACGCACTCTT TCATCGCCTT
    TATGGAATGT ACCCTTGCAA CTTCGTCTCC TTTTTGCGTT CTCATTACAG TATGAAAGAA
    AACCTGGAGA CTTTTGAAGA AGTGGTCAAG CCAATGATGG AGCATGTGCG AATTCATCCG
    GAATTAGTGA CTGGATCCAA GGACCATGAA CTGGACCCTC GAAGGTGGAA GAGATTAGAA
    ACTCATGATG TTGTGATCGA GTGTGCCAAA ATCTCTCTGG ATCCCACAGA AGCCTCATAT
    GAAGATGGCT ATTCTGTGTC TCACCAAATC TCAGCCCGCT TTCCTCATCG TTCAGCCGAT
    GTCACCACCA GCCCTTATGC TGACACACAG AATAGCTATG GGTGTGCTAC TTCTACCCCT
    TACTCCACGT CTCGGCTGAT GTTGTTAAAT ATGCCAGGGC AGCTACCTCA GACTCTGAGT
    TCCCCATCGA CACGGCTGAT AACTGAACCA CCACAAGCTA CTCTTTGGAG CCCATCTATG
    GTTTGTGGTA TGACCACTCC TCCAACTTCT CCTGGAAATG TCCCACCTGA TCTGTCACAC
    CCTTACAGTA AAGTCTTTGG TACAACTGCA GGTGGAAAAG GAACTCCTCT GGGAACCCCA
    GCAACCTCTC CTCCTCCAGC CCCACTCTGT CATTCGGATG ACTACGTGCA CATTTCACTC
    CCCCAGGCCA CAGTCACACC CCCCAGGAAG GAAGAGAGAA TGGATTCTGC AAGACCATGT
    CTACACAGAC AACACCATCT TCTGAATGAC AGAGGATCAG AAGAGCCACC TGGCAGCAAA
    GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG TTTTTAGGTG ATCTGGCCTC TGAAGAAGAT
    AGTATTGAAA AAGATAAAGA AGAAGCTGCA ATATCTAGAG AACTTTCTGA GATCACCACA
    GCAGAGGCAG AGCCTGTGGT TCCTCGAGGA GGCTTTGACT CTCCCTTTTA CCGAGACAGT
    CTCCCAGGTT CTCAGCGGAA GACCCACTCG GCAGCCTCCA GTTCTCAGGG CGCCAGCGTG
    AACCCTGAGC CTTTACACTC CTCCCTGGAC AAGCTTGGGC CTGACACACC AAAGCAAGCC
    TTTACTCCCA TAGACCTGCC CTGCGGCAGT GCTGATGAAA GCCCTGCGGG AGACAGGGAA
    TGCCAGACTT CTTTGGAGAC CAGTATCTTC ACTCCCAGTC CTTGTAAAAT TCCACCTCCG
    ACGAGAGTGG GCTTTGGAAG CGGGCAGCCT CCCCCGTATG ATCATCTTTT TGAGGTGGCA
    TTGCCAAAGA CAGCCCATCA TTTTGTCATC AGGAAGACTG AGGAGCTGTT AAAGAAAGCA
    AAAGGAAACA CAGAGGAAGA TGGTGTGCCC TCTACCTCCC CAATGGAAGT GCTGGACAGA
    CTGATACAGC AGGGAGCAGA CGCGCACAGC AAGGAGCTGA ACAAGTTGCC TTTACCCAGC
    AAGTCTGTCG ACTGGACCCA CTTTGGAGGC TCTCCTCCTT CAGATGAGAT CCGCACCCTC
    CGAGACCAGT TGCTTTTACT GCACAACCAG TTACTCTATG AGCGTTTTAA GAGGCAGCAG
    CATGCCCTCC GGAACAGGCG GCTCCTCCGC AAGGTGATCA AAGCAGCAGC TCTGGAGGAA
    CATAATGCTG CCATGAAAGA TCAGTTGAAG TTACAAGAGA AGGACATCCA GATGTGGAAG
    GTTAGTCTGC AGAAAGAACA AGCTAGATAC AATCAGCTCC AGGAGCAGCG TGACACTATG
    GTAACCAAGC TCCACAGCCA GATCAGACAG CTGCAGCATG ACCGAGAGGA ATTCTACAAC
    CAGAGCCAGG AATTACAGAC GAAGCTGGAG GACTGCAGGA ACATGATTGC GGAGCTGCGG
    ATAGAACTGA AGAAGGCCAA CAACAAGGTG TGTCACACTG AGCTGCTGCT CAGTCAGGTT
    TCCCAAAAGC TCTCAAACAG TGAGTCGGTC CAGCAGCAGA TGGAGTTCTT GAACAGGCAG
    CTGTTGGTTC TTGGGGAGGT CAACGAGCTC TATTTGGAAC AACTGCAGAA CAAGCACTCA
    GATACCACAA AGGAAGTAGA AATGATGAAA GCCGCCTATC GGAAAGAGCT AGAAAAAAAC
    AGAAGCCATG TTCTCCAGCA GACTCAGAGG CTTGATACCT CCCAAAAACG GATTTTGGAA
    CTGGAATCTC ACCTGGCCAA GAAAGACCAC CTTCTTTTGG AACAGAAGAA ATATCTAGAG
    GATGTCAAAC TCCAGGCAAG AGGACAGCTG CAGGCCGCAG AGAGCAGGTA TGAGGCTCAG
    AAAAGGATAA CCCAGGTGTT TGAATTGGAG ATCTTAGATT TATATGGCAG GTTGGAGAAA
    GATGGCCTCC TGAAAAAACT TGAAGAAGAA AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA
    AGGCTTGACT GTTGTAATGA CGGGTGCTCA GATTCCATGG TAGGGCACAA TGAAGAGGCA
    TCTGGCCACA ACGGTGAGAC CAAGACCCCC AGGCCCAGCA GCGCCCGGGG CAGTAGTGGA
    AGCAGAGGTG GTGGAGGCAG CAGCAGCAGC AGCAGCGAGC TTTCTACCCC AGAGAAACCC
    CCACACCAGA GGGCAGGCCC ATTCAGCAGT CGGTGGGAGA CGACTATGGG AGAAGCGTCT
    GCCAGCATCC CCACCACTGT GGGCTCACTT CCCAGTTCAA AAAGCTTCCT GGGTATGAAG
    GCTCGAGAGT TATTTCGTAA TAAGAGCGAG AGCCAGTGTG ATGAGGACGG CATGACCAGT
    AGCCTTTCTG AGAGCCTAAA GACAGAACTG GGCAAAGACT TGGGTGTGGA AGCCAAGATT
    CCCCTGAACC TAGATGGCCC TCACCCGTCT CCCCCGACCC CGGACAGTGT TGGACAGCTA
    CATATCATGG ACTACAATGA GACTCATCAT GAACACAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001155899.1
    CDS218..3559
    Translation

    Target ORF information:

    RefSeq Version NM_001162427.2
    Organism Homo sapiens(human)
    Definition Homo sapiens TSC complex subunit 1 (TSC1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001162427.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
    GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
    CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
    CTGACCACCT TGCAAGAGCC ACATGACAAG ATGGACACTG ACGTCGTTGT CCTCACAACA
    GGCGTCTTGG TGTTGATAAC CATGCTACCA ATGATTCCAC AGTCTGGGAA ACAGCATCTT
    CTTGATTTCT TTGACATTTT TGGCCGTCTG TCATCATGGT GCCTGAAGAA ACCAGGCCAC
    GTGGCGGAAG TCTATCTCGT CCATCTCCAT GCCAGTGTGT ACGCACTCTT TCATCGCCTT
    TATGGAATGT ACCCTTGCAA CTTCGTCTCC TTTTTGCGTT CTCATTACAG TATGAAAGAA
    AACCTGGAGA CTTTTGAAGA AGTGGTCAAG CCAATGATGG AGCATGTGCG AATTCATCCG
    GAATTAGTGA CTGGATCCAA GGACCATGAA CTGGACCCTC GAAGGTGGAA GAGATTAGAA
    ACTCATGATG TTGTGATCGA GTGTGCCAAA ATCTCTCTGG ATCCCACAGA AGCCTCATAT
    GAAGATGGCT ATTCTGTGTC TCACCAAATC TCAGCCCGCT TTCCTCATCG TTCAGCCGAT
    GTCACCACCA GCCCTTATGC TGACACACAG AATAGCTATG GGTGTGCTAC TTCTACCCCT
    TACTCCACGT CTCGGCTGAT GTTGTTAAAT ATGCCAGGGC AGCTACCTCA GACTCTGAGT
    TCCCCATCGA CACGGCTGAT AACTGAACCA CCACAAGCTA CTCTTTGGAG CCCATCTATG
    GTTTGTGGTA TGACCACTCC TCCAACTTCT CCTGGAAATG TCCCACCTGA TCTGTCACAC
    CCTTACAGTA AAGTCTTTGG TACAACTGCA GGTGGAAAAG GAACTCCTCT GGGAACCCCA
    GCAACCTCTC CTCCTCCAGC CCCACTCTGT CATTCGGATG ACTACGTGCA CATTTCACTC
    CCCCAGGCCA CAGTCACACC CCCCAGGAAG GAAGAGAGAA TGGATTCTGC AAGACCATGT
    CTACACAGAC AACACCATCT TCTGAATGAC AGAGGATCAG AAGAGCCACC TGGCAGCAAA
    GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG TTTTTAGGTG ATCTGGCCTC TGAAGAAGAT
    AGTATTGAAA AAGATAAAGA AGAAGCTGCA ATATCTAGAG AACTTTCTGA GATCACCACA
    GCAGAGGCAG AGCCTGTGGT TCCTCGAGGA GGCTTTGACT CTCCCTTTTA CCGAGACAGT
    CTCCCAGGTT CTCAGCGGAA GACCCACTCG GCAGCCTCCA GTTCTCAGGG CGCCAGCGTG
    AACCCTGAGC CTTTACACTC CTCCCTGGAC AAGCTTGGGC CTGACACACC AAAGCAAGCC
    TTTACTCCCA TAGACCTGCC CTGCGGCAGT GCTGATGAAA GCCCTGCGGG AGACAGGGAA
    TGCCAGACTT CTTTGGAGAC CAGTATCTTC ACTCCCAGTC CTTGTAAAAT TCCACCTCCG
    ACGAGAGTGG GCTTTGGAAG CGGGCAGCCT CCCCCGTATG ATCATCTTTT TGAGGTGGCA
    TTGCCAAAGA CAGCCCATCA TTTTGTCATC AGGAAGACTG AGGAGCTGTT AAAGAAAGCA
    AAAGGAAACA CAGAGGAAGA TGGTGTGCCC TCTACCTCCC CAATGGAAGT GCTGGACAGA
    CTGATACAGC AGGGAGCAGA CGCGCACAGC AAGGAGCTGA ACAAGTTGCC TTTACCCAGC
    AAGTCTGTCG ACTGGACCCA CTTTGGAGGC TCTCCTCCTT CAGATGAGAT CCGCACCCTC
    CGAGACCAGT TGCTTTTACT GCACAACCAG TTACTCTATG AGCGTTTTAA GAGGCAGCAG
    CATGCCCTCC GGAACAGGCG GCTCCTCCGC AAGGTGATCA AAGCAGCAGC TCTGGAGGAA
    CATAATGCTG CCATGAAAGA TCAGTTGAAG TTACAAGAGA AGGACATCCA GATGTGGAAG
    GTTAGTCTGC AGAAAGAACA AGCTAGATAC AATCAGCTCC AGGAGCAGCG TGACACTATG
    GTAACCAAGC TCCACAGCCA GATCAGACAG CTGCAGCATG ACCGAGAGGA ATTCTACAAC
    CAGAGCCAGG AATTACAGAC GAAGCTGGAG GACTGCAGGA ACATGATTGC GGAGCTGCGG
    ATAGAACTGA AGAAGGCCAA CAACAAGGTG TGTCACACTG AGCTGCTGCT CAGTCAGGTT
    TCCCAAAAGC TCTCAAACAG TGAGTCGGTC CAGCAGCAGA TGGAGTTCTT GAACAGGCAG
    CTGTTGGTTC TTGGGGAGGT CAACGAGCTC TATTTGGAAC AACTGCAGAA CAAGCACTCA
    GATACCACAA AGGAAGTAGA AATGATGAAA GCCGCCTATC GGAAAGAGCT AGAAAAAAAC
    AGAAGCCATG TTCTCCAGCA GACTCAGAGG CTTGATACCT CCCAAAAACG GATTTTGGAA
    CTGGAATCTC ACCTGGCCAA GAAAGACCAC CTTCTTTTGG AACAGAAGAA ATATCTAGAG
    GATGTCAAAC TCCAGGCAAG AGGACAGCTG CAGGCCGCAG AGAGCAGGTA TGAGGCTCAG
    AAAAGGATAA CCCAGGTGTT TGAATTGGAG ATCTTAGATT TATATGGCAG GTTGGAGAAA
    GATGGCCTCC TGAAAAAACT TGAAGAAGAA AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA
    AGGCTTGACT GTTGTAATGA CGGGTGCTCA GATTCCATGG TAGGGCACAA TGAAGAGGCA
    TCTGGCCACA ACGGTGAGAC CAAGACCCCC AGGCCCAGCA GCGCCCGGGG CAGTAGTGGA
    AGCAGAGGTG GTGGAGGCAG CAGCAGCAGC AGCAGCGAGC TTTCTACCCC AGAGAAACCC
    CCACACCAGA GGGCAGGCCC ATTCAGCAGT CGGTGGGAGA CGACTATGGG AGAAGCGTCT
    GCCAGCATCC CCACCACTGT GGGCTCACTT CCCAGTTCAA AAAGCTTCCT GGGTATGAAG
    GCTCGAGAGT TATTTCGTAA TAAGAGCGAG AGCCAGTGTG ATGAGGACGG CATGACCAGT
    AGCCTTTCTG AGAGCCTAAA GACAGAACTG GGCAAAGACT TGGGTGTGGA AGCCAAGATT
    CCCCTGAACC TAGATGGCCC TCACCCGTCT CCCCCGACCC CGGACAGTGT TGGACAGCTA
    CATATCATGG ACTACAATGA GACTCATCAT GAACACAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17622
    Clone ID Related Accession (Same CDS sequence) NM_001162426.1 , XM_017015098.1 , NM_001162426.2
    Accession Version NM_001162426.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3492bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-21
    Organism Homo sapiens(human)
    Product hamartin isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA726552.1, BC108668.1, AF013168.1, D87683.1 and N63914.1. Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.281943.1, SRR1803611.132989.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
    GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
    CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
    CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
    GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
    AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
    CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
    CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
    CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
    TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
    AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
    ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
    CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
    AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
    CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
    TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
    GGTGGAAAAG GAACTCCTCT GGGAACCCCA GCAACCTCTC CTCCTCCAGC CCCACTCTGT
    CATTCGGATG ACTACGTGCA CATTTCACTC CCCCAGGCCA CAGTCACACC CCCCAGGAAG
    GAAGAGAGAA TGGATTCTGC AAGACCATGT CTACACAGAC AACACCATCT TCTGAATGAC
    AGAGGATCAG AAGAGCCACC TGGCAGCAAA GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG
    TTTTTAGGTG ATCTGGCCTC TGAAGAAGAT AGTATTGAAA AAGATAAAGA AGAAGCTGCA
    ATATCTAGAG AACTTTCTGA GATCACCACA GCAGAGGCAG AGCCTGTGGT TCCTCGAGGA
    GGCTTTGACT CTCCCTTTTA CCGAGACAGT CTCCCAGGTT CTCAGCGGAA GACCCACTCG
    GCAGCCTCCA GTTCTCAGGG CGCCAGCGTG AACCCTGAGC CTTTACACTC CTCCCTGGAC
    AAGCTTGGGC CTGACACACC AAAGCAAGCC TTTACTCCCA TAGACCTGCC CTGCGGCAGT
    GCTGATGAAA GCCCTGCGGG AGACAGGGAA TGCCAGACTT CTTTGGAGAC CAGTATCTTC
    ACTCCCAGTC CTTGTAAAAT TCCACCTCCG ACGAGAGTGG GCTTTGGAAG CGGGCAGCCT
    CCCCCGTATG ATCATCTTTT TGAGGTGGCA TTGCCAAAGA CAGCCCATCA TTTTGTCATC
    AGGAAGACTG AGGAGCTGTT AAAGAAAGCA AAAGGAAACA CAGAGGAAGA TGGTGTGCCC
    TCTACCTCCC CAATGGAAGT GCTGGACAGA CTGATACAGC AGGGAGCAGA CGCGCACAGC
    AAGGAGCTGA ACAAGTTGCC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTTGGAGGC
    TCTCCTCCTT CAGATGAGAT CCGCACCCTC CGAGACCAGT TGCTTTTACT GCACAACCAG
    TTACTCTATG AGCGTTTTAA GAGGCAGCAG CATGCCCTCC GGAACAGGCG GCTCCTCCGC
    AAGGTGATCA AAGCAGCAGC TCTGGAGGAA CATAATGCTG CCATGAAAGA TCAGTTGAAG
    TTACAAGAGA AGGACATCCA GATGTGGAAG GTTAGTCTGC AGAAAGAACA AGCTAGATAC
    AATCAGCTCC AGGAGCAGCG TGACACTATG GTAACCAAGC TCCACAGCCA GATCAGACAG
    CTGCAGCATG ACCGAGAGGA ATTCTACAAC CAGAGCCAGG AATTACAGAC GAAGCTGGAG
    GACTGCAGGA ACATGATTGC GGAGCTGCGG ATAGAACTGA AGAAGGCCAA CAACAAGGTG
    TGTCACACTG AGCTGCTGCT CAGTCAGGTT TCCCAAAAGC TCTCAAACAG TGAGTCGGTC
    CAGCAGCAGA TGGAGTTCTT GAACAGGCAG CTGTTGGTTC TTGGGGAGGT CAACGAGCTC
    TATTTGGAAC AACTGCAGAA CAAGCACTCA GATACCACAA AGGAAGTAGA AATGATGAAA
    GCCGCCTATC GGAAAGAGCT AGAAAAAAAC AGAAGCCATG TTCTCCAGCA GACTCAGAGG
    CTTGATACCT CCCAAAAACG GATTTTGGAA CTGGAATCTC ACCTGGCCAA GAAAGACCAC
    CTTCTTTTGG AACAGAAGAA ATATCTAGAG GATGTCAAAC TCCAGGCAAG AGGACAGCTG
    CAGGCCGCAG AGAGCAGGTA TGAGGCTCAG AAAAGGATAA CCCAGGTGTT TGAATTGGAG
    ATCTTAGATT TATATGGCAG GTTGGAGAAA GATGGCCTCC TGAAAAAACT TGAAGAAGAA
    AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA AGGCTTGACT GTTGTAATGA CGGGTGCTCA
    GATTCCATGG TAGGGCACAA TGAAGAGGCA TCTGGCCACA ACGGTGAGAC CAAGACCCCC
    AGGCCCAGCA GCGCCCGGGG CAGTAGTGGA AGCAGAGGTG GTGGAGGCAG CAGCAGCAGC
    AGCAGCGAGC TTTCTACCCC AGAGAAACCC CCACACCAGA GGGCAGGCCC ATTCAGCAGT
    CGGTGGGAGA CGACTATGGG AGAAGCGTCT GCCAGCATCC CCACCACTGT GGGCTCACTT
    CCCAGTTCAA AAAGCTTCCT GGGTATGAAG GCTCGAGAGT TATTTCGTAA TAAGAGCGAG
    AGCCAGTGTG ATGAGGACGG CATGACCAGT AGCCTTTCTG AGAGCCTAAA GACAGAACTG
    GGCAAAGACT TGGGTGTGGA AGCCAAGATT CCCCTGAACC TAGATGGCCC TCACCCGTCT
    CCCCCGACCC CGGACAGTGT TGGACAGCTA CATATCATGG ACTACAATGA GACTCATCAT
    GAACACAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001155898.1
    CDS235..3726
    Translation

    Target ORF information:

    RefSeq Version NM_001162426.1
    Organism Homo sapiens(human)
    Definition Homo sapiens TSC complex subunit 1 (TSC1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001162426.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
    GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
    CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
    CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
    GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
    AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
    CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
    CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
    CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
    TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
    AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
    ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
    CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
    AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
    CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
    TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
    GGTGGAAAAG GAACTCCTCT GGGAACCCCA GCAACCTCTC CTCCTCCAGC CCCACTCTGT
    CATTCGGATG ACTACGTGCA CATTTCACTC CCCCAGGCCA CAGTCACACC CCCCAGGAAG
    GAAGAGAGAA TGGATTCTGC AAGACCATGT CTACACAGAC AACACCATCT TCTGAATGAC
    AGAGGATCAG AAGAGCCACC TGGCAGCAAA GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG
    TTTTTAGGTG ATCTGGCCTC TGAAGAAGAT AGTATTGAAA AAGATAAAGA AGAAGCTGCA
    ATATCTAGAG AACTTTCTGA GATCACCACA GCAGAGGCAG AGCCTGTGGT TCCTCGAGGA
    GGCTTTGACT CTCCCTTTTA CCGAGACAGT CTCCCAGGTT CTCAGCGGAA GACCCACTCG
    GCAGCCTCCA GTTCTCAGGG CGCCAGCGTG AACCCTGAGC CTTTACACTC CTCCCTGGAC
    AAGCTTGGGC CTGACACACC AAAGCAAGCC TTTACTCCCA TAGACCTGCC CTGCGGCAGT
    GCTGATGAAA GCCCTGCGGG AGACAGGGAA TGCCAGACTT CTTTGGAGAC CAGTATCTTC
    ACTCCCAGTC CTTGTAAAAT TCCACCTCCG ACGAGAGTGG GCTTTGGAAG CGGGCAGCCT
    CCCCCGTATG ATCATCTTTT TGAGGTGGCA TTGCCAAAGA CAGCCCATCA TTTTGTCATC
    AGGAAGACTG AGGAGCTGTT AAAGAAAGCA AAAGGAAACA CAGAGGAAGA TGGTGTGCCC
    TCTACCTCCC CAATGGAAGT GCTGGACAGA CTGATACAGC AGGGAGCAGA CGCGCACAGC
    AAGGAGCTGA ACAAGTTGCC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTTGGAGGC
    TCTCCTCCTT CAGATGAGAT CCGCACCCTC CGAGACCAGT TGCTTTTACT GCACAACCAG
    TTACTCTATG AGCGTTTTAA GAGGCAGCAG CATGCCCTCC GGAACAGGCG GCTCCTCCGC
    AAGGTGATCA AAGCAGCAGC TCTGGAGGAA CATAATGCTG CCATGAAAGA TCAGTTGAAG
    TTACAAGAGA AGGACATCCA GATGTGGAAG GTTAGTCTGC AGAAAGAACA AGCTAGATAC
    AATCAGCTCC AGGAGCAGCG TGACACTATG GTAACCAAGC TCCACAGCCA GATCAGACAG
    CTGCAGCATG ACCGAGAGGA ATTCTACAAC CAGAGCCAGG AATTACAGAC GAAGCTGGAG
    GACTGCAGGA ACATGATTGC GGAGCTGCGG ATAGAACTGA AGAAGGCCAA CAACAAGGTG
    TGTCACACTG AGCTGCTGCT CAGTCAGGTT TCCCAAAAGC TCTCAAACAG TGAGTCGGTC
    CAGCAGCAGA TGGAGTTCTT GAACAGGCAG CTGTTGGTTC TTGGGGAGGT CAACGAGCTC
    TATTTGGAAC AACTGCAGAA CAAGCACTCA GATACCACAA AGGAAGTAGA AATGATGAAA
    GCCGCCTATC GGAAAGAGCT AGAAAAAAAC AGAAGCCATG TTCTCCAGCA GACTCAGAGG
    CTTGATACCT CCCAAAAACG GATTTTGGAA CTGGAATCTC ACCTGGCCAA GAAAGACCAC
    CTTCTTTTGG AACAGAAGAA ATATCTAGAG GATGTCAAAC TCCAGGCAAG AGGACAGCTG
    CAGGCCGCAG AGAGCAGGTA TGAGGCTCAG AAAAGGATAA CCCAGGTGTT TGAATTGGAG
    ATCTTAGATT TATATGGCAG GTTGGAGAAA GATGGCCTCC TGAAAAAACT TGAAGAAGAA
    AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA AGGCTTGACT GTTGTAATGA CGGGTGCTCA
    GATTCCATGG TAGGGCACAA TGAAGAGGCA TCTGGCCACA ACGGTGAGAC CAAGACCCCC
    AGGCCCAGCA GCGCCCGGGG CAGTAGTGGA AGCAGAGGTG GTGGAGGCAG CAGCAGCAGC
    AGCAGCGAGC TTTCTACCCC AGAGAAACCC CCACACCAGA GGGCAGGCCC ATTCAGCAGT
    CGGTGGGAGA CGACTATGGG AGAAGCGTCT GCCAGCATCC CCACCACTGT GGGCTCACTT
    CCCAGTTCAA AAAGCTTCCT GGGTATGAAG GCTCGAGAGT TATTTCGTAA TAAGAGCGAG
    AGCCAGTGTG ATGAGGACGG CATGACCAGT AGCCTTTCTG AGAGCCTAAA GACAGAACTG
    GGCAAAGACT TGGGTGTGGA AGCCAAGATT CCCCTGAACC TAGATGGCCC TCACCCGTCT
    CCCCCGACCC CGGACAGTGT TGGACAGCTA CATATCATGG ACTACAATGA GACTCATCAT
    GAACACAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17622
    Clone ID Related Accession (Same CDS sequence) NM_001162426.1 , XM_017015098.1 , NM_001162426.2
    Accession Version NM_001162426.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3492bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-25
    Organism Homo sapiens(human)
    Product hamartin isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC108668.1, AF013168.1, D87683.1 and N63914.1. On Jun 2, 2019 this sequence version replaced NM_001162426.1. Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.132989.1, AK308412.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
    GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
    CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
    CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
    GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
    AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
    CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
    CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
    CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
    TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
    AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
    ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
    CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
    AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
    CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
    TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
    GGTGGAAAAG GAACTCCTCT GGGAACCCCA GCAACCTCTC CTCCTCCAGC CCCACTCTGT
    CATTCGGATG ACTACGTGCA CATTTCACTC CCCCAGGCCA CAGTCACACC CCCCAGGAAG
    GAAGAGAGAA TGGATTCTGC AAGACCATGT CTACACAGAC AACACCATCT TCTGAATGAC
    AGAGGATCAG AAGAGCCACC TGGCAGCAAA GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG
    TTTTTAGGTG ATCTGGCCTC TGAAGAAGAT AGTATTGAAA AAGATAAAGA AGAAGCTGCA
    ATATCTAGAG AACTTTCTGA GATCACCACA GCAGAGGCAG AGCCTGTGGT TCCTCGAGGA
    GGCTTTGACT CTCCCTTTTA CCGAGACAGT CTCCCAGGTT CTCAGCGGAA GACCCACTCG
    GCAGCCTCCA GTTCTCAGGG CGCCAGCGTG AACCCTGAGC CTTTACACTC CTCCCTGGAC
    AAGCTTGGGC CTGACACACC AAAGCAAGCC TTTACTCCCA TAGACCTGCC CTGCGGCAGT
    GCTGATGAAA GCCCTGCGGG AGACAGGGAA TGCCAGACTT CTTTGGAGAC CAGTATCTTC
    ACTCCCAGTC CTTGTAAAAT TCCACCTCCG ACGAGAGTGG GCTTTGGAAG CGGGCAGCCT
    CCCCCGTATG ATCATCTTTT TGAGGTGGCA TTGCCAAAGA CAGCCCATCA TTTTGTCATC
    AGGAAGACTG AGGAGCTGTT AAAGAAAGCA AAAGGAAACA CAGAGGAAGA TGGTGTGCCC
    TCTACCTCCC CAATGGAAGT GCTGGACAGA CTGATACAGC AGGGAGCAGA CGCGCACAGC
    AAGGAGCTGA ACAAGTTGCC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTTGGAGGC
    TCTCCTCCTT CAGATGAGAT CCGCACCCTC CGAGACCAGT TGCTTTTACT GCACAACCAG
    TTACTCTATG AGCGTTTTAA GAGGCAGCAG CATGCCCTCC GGAACAGGCG GCTCCTCCGC
    AAGGTGATCA AAGCAGCAGC TCTGGAGGAA CATAATGCTG CCATGAAAGA TCAGTTGAAG
    TTACAAGAGA AGGACATCCA GATGTGGAAG GTTAGTCTGC AGAAAGAACA AGCTAGATAC
    AATCAGCTCC AGGAGCAGCG TGACACTATG GTAACCAAGC TCCACAGCCA GATCAGACAG
    CTGCAGCATG ACCGAGAGGA ATTCTACAAC CAGAGCCAGG AATTACAGAC GAAGCTGGAG
    GACTGCAGGA ACATGATTGC GGAGCTGCGG ATAGAACTGA AGAAGGCCAA CAACAAGGTG
    TGTCACACTG AGCTGCTGCT CAGTCAGGTT TCCCAAAAGC TCTCAAACAG TGAGTCGGTC
    CAGCAGCAGA TGGAGTTCTT GAACAGGCAG CTGTTGGTTC TTGGGGAGGT CAACGAGCTC
    TATTTGGAAC AACTGCAGAA CAAGCACTCA GATACCACAA AGGAAGTAGA AATGATGAAA
    GCCGCCTATC GGAAAGAGCT AGAAAAAAAC AGAAGCCATG TTCTCCAGCA GACTCAGAGG
    CTTGATACCT CCCAAAAACG GATTTTGGAA CTGGAATCTC ACCTGGCCAA GAAAGACCAC
    CTTCTTTTGG AACAGAAGAA ATATCTAGAG GATGTCAAAC TCCAGGCAAG AGGACAGCTG
    CAGGCCGCAG AGAGCAGGTA TGAGGCTCAG AAAAGGATAA CCCAGGTGTT TGAATTGGAG
    ATCTTAGATT TATATGGCAG GTTGGAGAAA GATGGCCTCC TGAAAAAACT TGAAGAAGAA
    AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA AGGCTTGACT GTTGTAATGA CGGGTGCTCA
    GATTCCATGG TAGGGCACAA TGAAGAGGCA TCTGGCCACA ACGGTGAGAC CAAGACCCCC
    AGGCCCAGCA GCGCCCGGGG CAGTAGTGGA AGCAGAGGTG GTGGAGGCAG CAGCAGCAGC
    AGCAGCGAGC TTTCTACCCC AGAGAAACCC CCACACCAGA GGGCAGGCCC ATTCAGCAGT
    CGGTGGGAGA CGACTATGGG AGAAGCGTCT GCCAGCATCC CCACCACTGT GGGCTCACTT
    CCCAGTTCAA AAAGCTTCCT GGGTATGAAG GCTCGAGAGT TATTTCGTAA TAAGAGCGAG
    AGCCAGTGTG ATGAGGACGG CATGACCAGT AGCCTTTCTG AGAGCCTAAA GACAGAACTG
    GGCAAAGACT TGGGTGTGGA AGCCAAGATT CCCCTGAACC TAGATGGCCC TCACCCGTCT
    CCCCCGACCC CGGACAGTGT TGGACAGCTA CATATCATGG ACTACAATGA GACTCATCAT
    GAACACAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001155898.1
    CDS218..3709
    Translation

    Target ORF information:

    RefSeq Version NM_001162426.2
    Organism Homo sapiens(human)
    Definition Homo sapiens TSC complex subunit 1 (TSC1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001162426.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
    GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
    CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
    CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
    GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
    AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
    CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
    CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
    CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
    TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
    AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
    ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
    CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
    AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
    CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
    TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
    GGTGGAAAAG GAACTCCTCT GGGAACCCCA GCAACCTCTC CTCCTCCAGC CCCACTCTGT
    CATTCGGATG ACTACGTGCA CATTTCACTC CCCCAGGCCA CAGTCACACC CCCCAGGAAG
    GAAGAGAGAA TGGATTCTGC AAGACCATGT CTACACAGAC AACACCATCT TCTGAATGAC
    AGAGGATCAG AAGAGCCACC TGGCAGCAAA GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG
    TTTTTAGGTG ATCTGGCCTC TGAAGAAGAT AGTATTGAAA AAGATAAAGA AGAAGCTGCA
    ATATCTAGAG AACTTTCTGA GATCACCACA GCAGAGGCAG AGCCTGTGGT TCCTCGAGGA
    GGCTTTGACT CTCCCTTTTA CCGAGACAGT CTCCCAGGTT CTCAGCGGAA GACCCACTCG
    GCAGCCTCCA GTTCTCAGGG CGCCAGCGTG AACCCTGAGC CTTTACACTC CTCCCTGGAC
    AAGCTTGGGC CTGACACACC AAAGCAAGCC TTTACTCCCA TAGACCTGCC CTGCGGCAGT
    GCTGATGAAA GCCCTGCGGG AGACAGGGAA TGCCAGACTT CTTTGGAGAC CAGTATCTTC
    ACTCCCAGTC CTTGTAAAAT TCCACCTCCG ACGAGAGTGG GCTTTGGAAG CGGGCAGCCT
    CCCCCGTATG ATCATCTTTT TGAGGTGGCA TTGCCAAAGA CAGCCCATCA TTTTGTCATC
    AGGAAGACTG AGGAGCTGTT AAAGAAAGCA AAAGGAAACA CAGAGGAAGA TGGTGTGCCC
    TCTACCTCCC CAATGGAAGT GCTGGACAGA CTGATACAGC AGGGAGCAGA CGCGCACAGC
    AAGGAGCTGA ACAAGTTGCC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTTGGAGGC
    TCTCCTCCTT CAGATGAGAT CCGCACCCTC CGAGACCAGT TGCTTTTACT GCACAACCAG
    TTACTCTATG AGCGTTTTAA GAGGCAGCAG CATGCCCTCC GGAACAGGCG GCTCCTCCGC
    AAGGTGATCA AAGCAGCAGC TCTGGAGGAA CATAATGCTG CCATGAAAGA TCAGTTGAAG
    TTACAAGAGA AGGACATCCA GATGTGGAAG GTTAGTCTGC AGAAAGAACA AGCTAGATAC
    AATCAGCTCC AGGAGCAGCG TGACACTATG GTAACCAAGC TCCACAGCCA GATCAGACAG
    CTGCAGCATG ACCGAGAGGA ATTCTACAAC CAGAGCCAGG AATTACAGAC GAAGCTGGAG
    GACTGCAGGA ACATGATTGC GGAGCTGCGG ATAGAACTGA AGAAGGCCAA CAACAAGGTG
    TGTCACACTG AGCTGCTGCT CAGTCAGGTT TCCCAAAAGC TCTCAAACAG TGAGTCGGTC
    CAGCAGCAGA TGGAGTTCTT GAACAGGCAG CTGTTGGTTC TTGGGGAGGT CAACGAGCTC
    TATTTGGAAC AACTGCAGAA CAAGCACTCA GATACCACAA AGGAAGTAGA AATGATGAAA
    GCCGCCTATC GGAAAGAGCT AGAAAAAAAC AGAAGCCATG TTCTCCAGCA GACTCAGAGG
    CTTGATACCT CCCAAAAACG GATTTTGGAA CTGGAATCTC ACCTGGCCAA GAAAGACCAC
    CTTCTTTTGG AACAGAAGAA ATATCTAGAG GATGTCAAAC TCCAGGCAAG AGGACAGCTG
    CAGGCCGCAG AGAGCAGGTA TGAGGCTCAG AAAAGGATAA CCCAGGTGTT TGAATTGGAG
    ATCTTAGATT TATATGGCAG GTTGGAGAAA GATGGCCTCC TGAAAAAACT TGAAGAAGAA
    AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA AGGCTTGACT GTTGTAATGA CGGGTGCTCA
    GATTCCATGG TAGGGCACAA TGAAGAGGCA TCTGGCCACA ACGGTGAGAC CAAGACCCCC
    AGGCCCAGCA GCGCCCGGGG CAGTAGTGGA AGCAGAGGTG GTGGAGGCAG CAGCAGCAGC
    AGCAGCGAGC TTTCTACCCC AGAGAAACCC CCACACCAGA GGGCAGGCCC ATTCAGCAGT
    CGGTGGGAGA CGACTATGGG AGAAGCGTCT GCCAGCATCC CCACCACTGT GGGCTCACTT
    CCCAGTTCAA AAAGCTTCCT GGGTATGAAG GCTCGAGAGT TATTTCGTAA TAAGAGCGAG
    AGCCAGTGTG ATGAGGACGG CATGACCAGT AGCCTTTCTG AGAGCCTAAA GACAGAACTG
    GGCAAAGACT TGGGTGTGGA AGCCAAGATT CCCCTGAACC TAGATGGCCC TCACCCGTCT
    CCCCCGACCC CGGACAGTGT TGGACAGCTA CATATCATGG ACTACAATGA GACTCATCAT
    GAACACAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17622
    Clone ID Related Accession (Same CDS sequence) NM_001162426.1 , XM_017015098.1 , NM_001162426.2
    Accession Version XM_017015098.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3492bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product hamartin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
    GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
    CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
    CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
    GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
    AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
    CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
    CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
    CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
    TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
    AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
    ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
    CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
    AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
    CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
    TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
    GGTGGAAAAG GAACTCCTCT GGGAACCCCA GCAACCTCTC CTCCTCCAGC CCCACTCTGT
    CATTCGGATG ACTACGTGCA CATTTCACTC CCCCAGGCCA CAGTCACACC CCCCAGGAAG
    GAAGAGAGAA TGGATTCTGC AAGACCATGT CTACACAGAC AACACCATCT TCTGAATGAC
    AGAGGATCAG AAGAGCCACC TGGCAGCAAA GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG
    TTTTTAGGTG ATCTGGCCTC TGAAGAAGAT AGTATTGAAA AAGATAAAGA AGAAGCTGCA
    ATATCTAGAG AACTTTCTGA GATCACCACA GCAGAGGCAG AGCCTGTGGT TCCTCGAGGA
    GGCTTTGACT CTCCCTTTTA CCGAGACAGT CTCCCAGGTT CTCAGCGGAA GACCCACTCG
    GCAGCCTCCA GTTCTCAGGG CGCCAGCGTG AACCCTGAGC CTTTACACTC CTCCCTGGAC
    AAGCTTGGGC CTGACACACC AAAGCAAGCC TTTACTCCCA TAGACCTGCC CTGCGGCAGT
    GCTGATGAAA GCCCTGCGGG AGACAGGGAA TGCCAGACTT CTTTGGAGAC CAGTATCTTC
    ACTCCCAGTC CTTGTAAAAT TCCACCTCCG ACGAGAGTGG GCTTTGGAAG CGGGCAGCCT
    CCCCCGTATG ATCATCTTTT TGAGGTGGCA TTGCCAAAGA CAGCCCATCA TTTTGTCATC
    AGGAAGACTG AGGAGCTGTT AAAGAAAGCA AAAGGAAACA CAGAGGAAGA TGGTGTGCCC
    TCTACCTCCC CAATGGAAGT GCTGGACAGA CTGATACAGC AGGGAGCAGA CGCGCACAGC
    AAGGAGCTGA ACAAGTTGCC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTTGGAGGC
    TCTCCTCCTT CAGATGAGAT CCGCACCCTC CGAGACCAGT TGCTTTTACT GCACAACCAG
    TTACTCTATG AGCGTTTTAA GAGGCAGCAG CATGCCCTCC GGAACAGGCG GCTCCTCCGC
    AAGGTGATCA AAGCAGCAGC TCTGGAGGAA CATAATGCTG CCATGAAAGA TCAGTTGAAG
    TTACAAGAGA AGGACATCCA GATGTGGAAG GTTAGTCTGC AGAAAGAACA AGCTAGATAC
    AATCAGCTCC AGGAGCAGCG TGACACTATG GTAACCAAGC TCCACAGCCA GATCAGACAG
    CTGCAGCATG ACCGAGAGGA ATTCTACAAC CAGAGCCAGG AATTACAGAC GAAGCTGGAG
    GACTGCAGGA ACATGATTGC GGAGCTGCGG ATAGAACTGA AGAAGGCCAA CAACAAGGTG
    TGTCACACTG AGCTGCTGCT CAGTCAGGTT TCCCAAAAGC TCTCAAACAG TGAGTCGGTC
    CAGCAGCAGA TGGAGTTCTT GAACAGGCAG CTGTTGGTTC TTGGGGAGGT CAACGAGCTC
    TATTTGGAAC AACTGCAGAA CAAGCACTCA GATACCACAA AGGAAGTAGA AATGATGAAA
    GCCGCCTATC GGAAAGAGCT AGAAAAAAAC AGAAGCCATG TTCTCCAGCA GACTCAGAGG
    CTTGATACCT CCCAAAAACG GATTTTGGAA CTGGAATCTC ACCTGGCCAA GAAAGACCAC
    CTTCTTTTGG AACAGAAGAA ATATCTAGAG GATGTCAAAC TCCAGGCAAG AGGACAGCTG
    CAGGCCGCAG AGAGCAGGTA TGAGGCTCAG AAAAGGATAA CCCAGGTGTT TGAATTGGAG
    ATCTTAGATT TATATGGCAG GTTGGAGAAA GATGGCCTCC TGAAAAAACT TGAAGAAGAA
    AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA AGGCTTGACT GTTGTAATGA CGGGTGCTCA
    GATTCCATGG TAGGGCACAA TGAAGAGGCA TCTGGCCACA ACGGTGAGAC CAAGACCCCC
    AGGCCCAGCA GCGCCCGGGG CAGTAGTGGA AGCAGAGGTG GTGGAGGCAG CAGCAGCAGC
    AGCAGCGAGC TTTCTACCCC AGAGAAACCC CCACACCAGA GGGCAGGCCC ATTCAGCAGT
    CGGTGGGAGA CGACTATGGG AGAAGCGTCT GCCAGCATCC CCACCACTGT GGGCTCACTT
    CCCAGTTCAA AAAGCTTCCT GGGTATGAAG GCTCGAGAGT TATTTCGTAA TAAGAGCGAG
    AGCCAGTGTG ATGAGGACGG CATGACCAGT AGCCTTTCTG AGAGCCTAAA GACAGAACTG
    GGCAAAGACT TGGGTGTGGA AGCCAAGATT CCCCTGAACC TAGATGGCCC TCACCCGTCT
    CCCCCGACCC CGGACAGTGT TGGACAGCTA CATATCATGG ACTACAATGA GACTCATCAT
    GAACACAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016870587.1
    CDS280..3771
    Translation

    Target ORF information:

    RefSeq Version XM_017015098.1
    Organism Homo sapiens(human)
    Definition Homo sapiens TSC complex subunit 1 (TSC1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017015098.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
    GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
    CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
    CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
    GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
    CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
    AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
    CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
    CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
    CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
    TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
    AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
    GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
    AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
    ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
    CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
    AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
    CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
    TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
    GGTGGAAAAG GAACTCCTCT GGGAACCCCA GCAACCTCTC CTCCTCCAGC CCCACTCTGT
    CATTCGGATG ACTACGTGCA CATTTCACTC CCCCAGGCCA CAGTCACACC CCCCAGGAAG
    GAAGAGAGAA TGGATTCTGC AAGACCATGT CTACACAGAC AACACCATCT TCTGAATGAC
    AGAGGATCAG AAGAGCCACC TGGCAGCAAA GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG
    TTTTTAGGTG ATCTGGCCTC TGAAGAAGAT AGTATTGAAA AAGATAAAGA AGAAGCTGCA
    ATATCTAGAG AACTTTCTGA GATCACCACA GCAGAGGCAG AGCCTGTGGT TCCTCGAGGA
    GGCTTTGACT CTCCCTTTTA CCGAGACAGT CTCCCAGGTT CTCAGCGGAA GACCCACTCG
    GCAGCCTCCA GTTCTCAGGG CGCCAGCGTG AACCCTGAGC CTTTACACTC CTCCCTGGAC
    AAGCTTGGGC CTGACACACC AAAGCAAGCC TTTACTCCCA TAGACCTGCC CTGCGGCAGT
    GCTGATGAAA GCCCTGCGGG AGACAGGGAA TGCCAGACTT CTTTGGAGAC CAGTATCTTC
    ACTCCCAGTC CTTGTAAAAT TCCACCTCCG ACGAGAGTGG GCTTTGGAAG CGGGCAGCCT
    CCCCCGTATG ATCATCTTTT TGAGGTGGCA TTGCCAAAGA CAGCCCATCA TTTTGTCATC
    AGGAAGACTG AGGAGCTGTT AAAGAAAGCA AAAGGAAACA CAGAGGAAGA TGGTGTGCCC
    TCTACCTCCC CAATGGAAGT GCTGGACAGA CTGATACAGC AGGGAGCAGA CGCGCACAGC
    AAGGAGCTGA ACAAGTTGCC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTTGGAGGC
    TCTCCTCCTT CAGATGAGAT CCGCACCCTC CGAGACCAGT TGCTTTTACT GCACAACCAG
    TTACTCTATG AGCGTTTTAA GAGGCAGCAG CATGCCCTCC GGAACAGGCG GCTCCTCCGC
    AAGGTGATCA AAGCAGCAGC TCTGGAGGAA CATAATGCTG CCATGAAAGA TCAGTTGAAG
    TTACAAGAGA AGGACATCCA GATGTGGAAG GTTAGTCTGC AGAAAGAACA AGCTAGATAC
    AATCAGCTCC AGGAGCAGCG TGACACTATG GTAACCAAGC TCCACAGCCA GATCAGACAG
    CTGCAGCATG ACCGAGAGGA ATTCTACAAC CAGAGCCAGG AATTACAGAC GAAGCTGGAG
    GACTGCAGGA ACATGATTGC GGAGCTGCGG ATAGAACTGA AGAAGGCCAA CAACAAGGTG
    TGTCACACTG AGCTGCTGCT CAGTCAGGTT TCCCAAAAGC TCTCAAACAG TGAGTCGGTC
    CAGCAGCAGA TGGAGTTCTT GAACAGGCAG CTGTTGGTTC TTGGGGAGGT CAACGAGCTC
    TATTTGGAAC AACTGCAGAA CAAGCACTCA GATACCACAA AGGAAGTAGA AATGATGAAA
    GCCGCCTATC GGAAAGAGCT AGAAAAAAAC AGAAGCCATG TTCTCCAGCA GACTCAGAGG
    CTTGATACCT CCCAAAAACG GATTTTGGAA CTGGAATCTC ACCTGGCCAA GAAAGACCAC
    CTTCTTTTGG AACAGAAGAA ATATCTAGAG GATGTCAAAC TCCAGGCAAG AGGACAGCTG
    CAGGCCGCAG AGAGCAGGTA TGAGGCTCAG AAAAGGATAA CCCAGGTGTT TGAATTGGAG
    ATCTTAGATT TATATGGCAG GTTGGAGAAA GATGGCCTCC TGAAAAAACT TGAAGAAGAA
    AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA AGGCTTGACT GTTGTAATGA CGGGTGCTCA
    GATTCCATGG TAGGGCACAA TGAAGAGGCA TCTGGCCACA ACGGTGAGAC CAAGACCCCC
    AGGCCCAGCA GCGCCCGGGG CAGTAGTGGA AGCAGAGGTG GTGGAGGCAG CAGCAGCAGC
    AGCAGCGAGC TTTCTACCCC AGAGAAACCC CCACACCAGA GGGCAGGCCC ATTCAGCAGT
    CGGTGGGAGA CGACTATGGG AGAAGCGTCT GCCAGCATCC CCACCACTGT GGGCTCACTT
    CCCAGTTCAA AAAGCTTCCT GGGTATGAAG GCTCGAGAGT TATTTCGTAA TAAGAGCGAG
    AGCCAGTGTG ATGAGGACGG CATGACCAGT AGCCTTTCTG AGAGCCTAAA GACAGAACTG
    GGCAAAGACT TGGGTGTGGA AGCCAAGATT CCCCTGAACC TAGATGGCCC TCACCCGTCT
    CCCCCGACCC CGGACAGTGT TGGACAGCTA CATATCATGG ACTACAATGA GACTCATCAT
    GAACACAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.