LRRC8E cDNA ORF clone, Homo sapiens(Human)

  • Gene

  • Clones

  • gRNAs

The following LRRC8E gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LRRC8E cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu00041 NM_025061.6
Latest version!
Homo sapiens leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
OHu00041 NM_001268284.2
Latest version!
Homo sapiens leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
OHu29088 NM_001268285.3
Latest version!
Homo sapiens leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
OHu00041 XM_011528319.2
Latest version!
Homo sapiens leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
View Old Accession Versions >>
OHu00041 NM_025061.5 Homo sapiens leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
OHu29088 NM_001268285.2 Homo sapiens leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
OHu00041 XM_011528319.1 Homo sapiens leucine rich repeat containing 8 family, member E (LRRC8E), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu00041
    Clone ID Related Accession (Same CDS sequence) NM_001268284.2 , NM_025061.6 , NM_025061.5 , XM_011528319.2 , XM_011528319.1
    Accession Version NM_001268284.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2391bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Homo sapiens(human)
    Product volume-regulated anion channel subunit LRRC8E isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK091134.1, CF593782.1, KF459574.1, BX538180.1, AC010336.8 and CB049178.1. This sequence is a reference standard in the RefSeqGene project. On Jan 17, 2014 this sequence version replaced NM_001268284.1. Transcript Variant: This variant (2) contains an additional exon in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein (isoform 1). ##Evidence-Data-START## Transcript exon combination :: CF593782.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2158569, SAMN02400288 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATCCCAG TGGCCGAGTT CAAGCAGTTC ACGGAACAGC AGCCTGCGTT CAAGGTGCTC 
    AAACCCTGGT GGGACGTGCT GGCCGAGTAC CTCACCGTGG CCATGCTCAT GATTGGGGTC
    TTTGGCTGCA CCCTCCAGGT GACACAGGAC AAGATCATCT GTCTACCCAA TCATGAGCTC
    CAGGAGAACT TATCAGAGGC CCCGTGCCAG CAATTGCTGC CTCGGGGGAT CCCTGAGCAG
    ATTGGGGCCC TGCAGGAGGT TAAAGGCCTT AAGAACAATT TGGACCTGCA GCAATACAGC
    TTTATTAACC AGCTGTGTTA TGAGACGGCC CTGCACTGGT ATGCCAAGTA CTTCCCTTAC
    CTCGTGGTCA TTCACACACT CATCTTCATG GTCTGCACCA GTTTCTGGTT CAAGTTCCCT
    GGCACCAGCT CCAAGATTGA ACACTTCATC TCCATCCTGG GCAAGTGTTT CGACTCTCCA
    TGGACCACCA GGGCCCTATC CGAGGTCTCC GGGGAGAACC AGAAGGGCCC AGCAGCCACC
    GAACGGGCTG CGGCCACCAT AGTGGCCATG GCAGGGACCG GGCCGGGGAA GGCAGGGGAG
    GGTGAGAAGG AGAAAGTGCT GGCGGAACCG GAGAAGGTGG TGACCGAGCC TCCAGTTGTC
    ACCCTGTTGG ACAAGAAGGA GGGTGAGCAA GCCAAAGCCC TGTTTGAGAA GGTGAAGAAG
    TTCCGCATGC ACGTGGAAGA GGGCGACATC CTGTACACCA TGTACATCCG ACAGACGGTG
    CTGAAAGTGT GTAAGTTCCT GGCCATCCTG GTCTACAACC TGGTCTATGT GGAGAAGATC
    AGTTTCCTGG TGGCCTGTAG GGTGGAGACG TCAGAGGTCA CGGGCTACGC CAGCTTCTGC
    TGCAACCACA CCAAGGCCCA CCTCTTCTCC AAGCTGGCCT TCTGTTACAT CTCCTTTGTG
    TGCATCTACG GACTTACCTG CATCTACACG CTCTACTGGC TCTTCCACCG GCCCCTCAAG
    GAGTACTCCT TCCGTTCCGT GCGGGAGGAG ACTGGCATGG GGGACATTCC TGACGTCAAG
    AATGACTTCG CCTTCATGCT GCACCTCATC GATCAGTACG ACTCCCTCTA CTCCAAGCGC
    TTCGCCGTCT TCCTGTCCGA GGTCAGCGAA AGCCGTCTAA AGCAGCTCAA TCTCAACCAC
    GAGTGGACGC CCGAGAAGCT TCGACAGAAG CTGCAGCGCA ATGCCGCGGG CCGGCTGGAG
    CTGGCCCTCT GCATGCTGCC GGGTCTGCCC GACACCGTCT TTGAGCTCAG TGAGGTGGAG
    TCACTCAGGC TGGAGGCCAT CTGCGATATC ACCTTCCCCC CGGGGCTGTC ACAGCTGGTG
    CACTTGCAGG AGCTCAGCTT GCTCCACTCG CCCGCCAGGC TACCCTTCTC CTTGCAGGTC
    TTCCTGCGGG ACCACCTGAA GGTGATGCGC GTCAAATGCG AGGAGCTCCG CGAGGTGCCG
    CTTTGGGTGT TTGGGCTGCG GGGCTTGGAG GAGCTGCACC TGGAGGGGCT TTTCCCCCAG
    GAGCTAGCTC GGGCAGCCAC CCTGGAGAGC CTCCGGGAGC TGAAGCAGCT CAAGGTGTTG
    TCCCTCCGGA GCAACGCCGG GAAGGTGCCA GCCAGTGTGA CCGACGTTGC TGGCCACCTG
    CAGAGGCTCA GCCTGCACAA CGATGGGGCC CGTCTGGTTG CCCTGAACAG CCTCAAGAAG
    CTGGCGGCAT TGCGGGAGCT GGAGCTGGTG GCCTGCGGGC TGGAGCGCAT CCCCCATGCA
    GTGTTCAGCC TGGGTGCGCT GCAGGAACTT GACCTCAAGG ACAACCACCT GCGCTCCATC
    GAGGAAATCC TCAGCTTCCA GCACTGCCGG AAGCTGGTCA CGCTCAGGCT GTGGCACAAC
    CAGATCGCCT ACGTCCCTGA GCACGTGCGG AAGCTCAGGA GCCTGGAGCA GCTCTACCTC
    AGCTACAACA AGCTGGAGAC CCTGCCCTCC CAGCTCGGCC TGTGCTCAGG CCTCCGTCTG
    CTGGATGTGT CCCACAATGG GCTACACTCC CTGCCACCCG AGGTGGGCCT CCTGCAGAAC
    CTACAGCACC TGGCCCTCTC CTACAATGCC CTGGAGGCCC TGCCCGAAGA GCTCTTCTTC
    TGCCGCAAGC TGCGGACGTT GCTTCTGGGC GACAACCAGC TGAGCCAGCT CTCGCCCCAC
    GTGGGTGCCC TCAGAGCCCT CAGCCGCCTG GAGCTCAAAG GCAACCGCTT AGAGGCGCTG
    CCAGAAGAAC TTGGCAACTG TGGGGGGCTC AAGAAGGCGG GGCTCCTGGT GGAAGACACG
    CTTTACCAGG GTCTGCCGGC AGAAGTGCGG GACAAGATGG AGGAGGAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255213.1
    CDS245..2635
    Translation

    Target ORF information:

    RefSeq Version NM_001268284.2
    Organism Homo sapiens(human)
    Definition Homo sapiens leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268284.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATCCCAG TGGCCGAGTT CAAGCAGTTC ACGGAACAGC AGCCTGCGTT CAAGGTGCTC 
    AAACCCTGGT GGGACGTGCT GGCCGAGTAC CTCACCGTGG CCATGCTCAT GATTGGGGTC
    TTTGGCTGCA CCCTCCAGGT GACACAGGAC AAGATCATCT GTCTACCCAA TCATGAGCTC
    CAGGAGAACT TATCAGAGGC CCCGTGCCAG CAATTGCTGC CTCGGGGGAT CCCTGAGCAG
    ATTGGGGCCC TGCAGGAGGT TAAAGGCCTT AAGAACAATT TGGACCTGCA GCAATACAGC
    TTTATTAACC AGCTGTGTTA TGAGACGGCC CTGCACTGGT ATGCCAAGTA CTTCCCTTAC
    CTCGTGGTCA TTCACACACT CATCTTCATG GTCTGCACCA GTTTCTGGTT CAAGTTCCCT
    GGCACCAGCT CCAAGATTGA ACACTTCATC TCCATCCTGG GCAAGTGTTT CGACTCTCCA
    TGGACCACCA GGGCCCTATC CGAGGTCTCC GGGGAGAACC AGAAGGGCCC AGCAGCCACC
    GAACGGGCTG CGGCCACCAT AGTGGCCATG GCAGGGACCG GGCCGGGGAA GGCAGGGGAG
    GGTGAGAAGG AGAAAGTGCT GGCGGAACCG GAGAAGGTGG TGACCGAGCC TCCAGTTGTC
    ACCCTGTTGG ACAAGAAGGA GGGTGAGCAA GCCAAAGCCC TGTTTGAGAA GGTGAAGAAG
    TTCCGCATGC ACGTGGAAGA GGGCGACATC CTGTACACCA TGTACATCCG ACAGACGGTG
    CTGAAAGTGT GTAAGTTCCT GGCCATCCTG GTCTACAACC TGGTCTATGT GGAGAAGATC
    AGTTTCCTGG TGGCCTGTAG GGTGGAGACG TCAGAGGTCA CGGGCTACGC CAGCTTCTGC
    TGCAACCACA CCAAGGCCCA CCTCTTCTCC AAGCTGGCCT TCTGTTACAT CTCCTTTGTG
    TGCATCTACG GACTTACCTG CATCTACACG CTCTACTGGC TCTTCCACCG GCCCCTCAAG
    GAGTACTCCT TCCGTTCCGT GCGGGAGGAG ACTGGCATGG GGGACATTCC TGACGTCAAG
    AATGACTTCG CCTTCATGCT GCACCTCATC GATCAGTACG ACTCCCTCTA CTCCAAGCGC
    TTCGCCGTCT TCCTGTCCGA GGTCAGCGAA AGCCGTCTAA AGCAGCTCAA TCTCAACCAC
    GAGTGGACGC CCGAGAAGCT TCGACAGAAG CTGCAGCGCA ATGCCGCGGG CCGGCTGGAG
    CTGGCCCTCT GCATGCTGCC GGGTCTGCCC GACACCGTCT TTGAGCTCAG TGAGGTGGAG
    TCACTCAGGC TGGAGGCCAT CTGCGATATC ACCTTCCCCC CGGGGCTGTC ACAGCTGGTG
    CACTTGCAGG AGCTCAGCTT GCTCCACTCG CCCGCCAGGC TACCCTTCTC CTTGCAGGTC
    TTCCTGCGGG ACCACCTGAA GGTGATGCGC GTCAAATGCG AGGAGCTCCG CGAGGTGCCG
    CTTTGGGTGT TTGGGCTGCG GGGCTTGGAG GAGCTGCACC TGGAGGGGCT TTTCCCCCAG
    GAGCTAGCTC GGGCAGCCAC CCTGGAGAGC CTCCGGGAGC TGAAGCAGCT CAAGGTGTTG
    TCCCTCCGGA GCAACGCCGG GAAGGTGCCA GCCAGTGTGA CCGACGTTGC TGGCCACCTG
    CAGAGGCTCA GCCTGCACAA CGATGGGGCC CGTCTGGTTG CCCTGAACAG CCTCAAGAAG
    CTGGCGGCAT TGCGGGAGCT GGAGCTGGTG GCCTGCGGGC TGGAGCGCAT CCCCCATGCA
    GTGTTCAGCC TGGGTGCGCT GCAGGAACTT GACCTCAAGG ACAACCACCT GCGCTCCATC
    GAGGAAATCC TCAGCTTCCA GCACTGCCGG AAGCTGGTCA CGCTCAGGCT GTGGCACAAC
    CAGATCGCCT ACGTCCCTGA GCACGTGCGG AAGCTCAGGA GCCTGGAGCA GCTCTACCTC
    AGCTACAACA AGCTGGAGAC CCTGCCCTCC CAGCTCGGCC TGTGCTCAGG CCTCCGTCTG
    CTGGATGTGT CCCACAATGG GCTACACTCC CTGCCACCCG AGGTGGGCCT CCTGCAGAAC
    CTACAGCACC TGGCCCTCTC CTACAATGCC CTGGAGGCCC TGCCCGAAGA GCTCTTCTTC
    TGCCGCAAGC TGCGGACGTT GCTTCTGGGC GACAACCAGC TGAGCCAGCT CTCGCCCCAC
    GTGGGTGCCC TCAGAGCCCT CAGCCGCCTG GAGCTCAAAG GCAACCGCTT AGAGGCGCTG
    CCAGAAGAAC TTGGCAACTG TGGGGGGCTC AAGAAGGCGG GGCTCCTGGT GGAAGACACG
    CTTTACCAGG GTCTGCCGGC AGAAGTGCGG GACAAGATGG AGGAGGAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu00041
    Clone ID Related Accession (Same CDS sequence) NM_001268284.2 , NM_025061.6 , NM_025061.5 , XM_011528319.2 , XM_011528319.1
    Accession Version NM_025061.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2391bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-05-01
    Organism Homo sapiens(human)
    Product volume-regulated anion channel subunit LRRC8E isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK091134.1, KF459574.1, BX538180.1 and CB049178.1. On Nov 22, 2018 this sequence version replaced NM_025061.5. Transcript Variant: This variant (1) encodes the longer isoform (1). Variants 1 and 2 encode the same protein (isoform 1). ##Evidence-Data-START## Transcript exon combination :: BX538180.1, BC070089.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000306708.11/ ENSP00000306524.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATCCCAG TGGCCGAGTT CAAGCAGTTC ACGGAACAGC AGCCTGCGTT CAAGGTGCTC 
    AAACCCTGGT GGGACGTGCT GGCCGAGTAC CTCACCGTGG CCATGCTCAT GATTGGGGTC
    TTTGGCTGCA CCCTCCAGGT GACACAGGAC AAGATCATCT GTCTACCCAA TCATGAGCTC
    CAGGAGAACT TATCAGAGGC CCCGTGCCAG CAATTGCTGC CTCGGGGGAT CCCTGAGCAG
    ATTGGGGCCC TGCAGGAGGT TAAAGGCCTT AAGAACAATT TGGACCTGCA GCAATACAGC
    TTTATTAACC AGCTGTGTTA TGAGACGGCC CTGCACTGGT ATGCCAAGTA CTTCCCTTAC
    CTCGTGGTCA TTCACACACT CATCTTCATG GTCTGCACCA GTTTCTGGTT CAAGTTCCCT
    GGCACCAGCT CCAAGATTGA ACACTTCATC TCCATCCTGG GCAAGTGTTT CGACTCTCCA
    TGGACCACCA GGGCCCTATC CGAGGTCTCC GGGGAGAACC AGAAGGGCCC AGCAGCCACC
    GAACGGGCTG CGGCCACCAT AGTGGCCATG GCAGGGACCG GGCCGGGGAA GGCAGGGGAG
    GGTGAGAAGG AGAAAGTGCT GGCGGAACCG GAGAAGGTGG TGACCGAGCC TCCAGTTGTC
    ACCCTGTTGG ACAAGAAGGA GGGTGAGCAA GCCAAAGCCC TGTTTGAGAA GGTGAAGAAG
    TTCCGCATGC ACGTGGAAGA GGGCGACATC CTGTACACCA TGTACATCCG ACAGACGGTG
    CTGAAAGTGT GTAAGTTCCT GGCCATCCTG GTCTACAACC TGGTCTATGT GGAGAAGATC
    AGTTTCCTGG TGGCCTGTAG GGTGGAGACG TCAGAGGTCA CGGGCTACGC CAGCTTCTGC
    TGCAACCACA CCAAGGCCCA CCTCTTCTCC AAGCTGGCCT TCTGTTACAT CTCCTTTGTG
    TGCATCTACG GACTTACCTG CATCTACACG CTCTACTGGC TCTTCCACCG GCCCCTCAAG
    GAGTACTCCT TCCGTTCCGT GCGGGAGGAG ACTGGCATGG GGGACATTCC TGACGTCAAG
    AATGACTTCG CCTTCATGCT GCACCTCATC GATCAGTACG ACTCCCTCTA CTCCAAGCGC
    TTCGCCGTCT TCCTGTCCGA GGTCAGCGAA AGCCGTCTAA AGCAGCTCAA TCTCAACCAC
    GAGTGGACGC CCGAGAAGCT TCGACAGAAG CTGCAGCGCA ATGCCGCGGG CCGGCTGGAG
    CTGGCCCTCT GCATGCTGCC GGGTCTGCCC GACACCGTCT TTGAGCTCAG TGAGGTGGAG
    TCACTCAGGC TGGAGGCCAT CTGCGATATC ACCTTCCCCC CGGGGCTGTC ACAGCTGGTG
    CACTTGCAGG AGCTCAGCTT GCTCCACTCG CCCGCCAGGC TACCCTTCTC CTTGCAGGTC
    TTCCTGCGGG ACCACCTGAA GGTGATGCGC GTCAAATGCG AGGAGCTCCG CGAGGTGCCG
    CTTTGGGTGT TTGGGCTGCG GGGCTTGGAG GAGCTGCACC TGGAGGGGCT TTTCCCCCAG
    GAGCTAGCTC GGGCAGCCAC CCTGGAGAGC CTCCGGGAGC TGAAGCAGCT CAAGGTGTTG
    TCCCTCCGGA GCAACGCCGG GAAGGTGCCA GCCAGTGTGA CCGACGTTGC TGGCCACCTG
    CAGAGGCTCA GCCTGCACAA CGATGGGGCC CGTCTGGTTG CCCTGAACAG CCTCAAGAAG
    CTGGCGGCAT TGCGGGAGCT GGAGCTGGTG GCCTGCGGGC TGGAGCGCAT CCCCCATGCA
    GTGTTCAGCC TGGGTGCGCT GCAGGAACTT GACCTCAAGG ACAACCACCT GCGCTCCATC
    GAGGAAATCC TCAGCTTCCA GCACTGCCGG AAGCTGGTCA CGCTCAGGCT GTGGCACAAC
    CAGATCGCCT ACGTCCCTGA GCACGTGCGG AAGCTCAGGA GCCTGGAGCA GCTCTACCTC
    AGCTACAACA AGCTGGAGAC CCTGCCCTCC CAGCTCGGCC TGTGCTCAGG CCTCCGTCTG
    CTGGATGTGT CCCACAATGG GCTACACTCC CTGCCACCCG AGGTGGGCCT CCTGCAGAAC
    CTACAGCACC TGGCCCTCTC CTACAATGCC CTGGAGGCCC TGCCCGAAGA GCTCTTCTTC
    TGCCGCAAGC TGCGGACGTT GCTTCTGGGC GACAACCAGC TGAGCCAGCT CTCGCCCCAC
    GTGGGTGCCC TCAGAGCCCT CAGCCGCCTG GAGCTCAAAG GCAACCGCTT AGAGGCGCTG
    CCAGAAGAAC TTGGCAACTG TGGGGGGCTC AAGAAGGCGG GGCTCCTGGT GGAAGACACG
    CTTTACCAGG GTCTGCCGGC AGAAGTGCGG GACAAGATGG AGGAGGAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_079337.2
    CDS97..2487
    Translation

    Target ORF information:

    RefSeq Version NM_025061.6
    Organism Homo sapiens(human)
    Definition Homo sapiens leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_025061.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATCCCAG TGGCCGAGTT CAAGCAGTTC ACGGAACAGC AGCCTGCGTT CAAGGTGCTC 
    AAACCCTGGT GGGACGTGCT GGCCGAGTAC CTCACCGTGG CCATGCTCAT GATTGGGGTC
    TTTGGCTGCA CCCTCCAGGT GACACAGGAC AAGATCATCT GTCTACCCAA TCATGAGCTC
    CAGGAGAACT TATCAGAGGC CCCGTGCCAG CAATTGCTGC CTCGGGGGAT CCCTGAGCAG
    ATTGGGGCCC TGCAGGAGGT TAAAGGCCTT AAGAACAATT TGGACCTGCA GCAATACAGC
    TTTATTAACC AGCTGTGTTA TGAGACGGCC CTGCACTGGT ATGCCAAGTA CTTCCCTTAC
    CTCGTGGTCA TTCACACACT CATCTTCATG GTCTGCACCA GTTTCTGGTT CAAGTTCCCT
    GGCACCAGCT CCAAGATTGA ACACTTCATC TCCATCCTGG GCAAGTGTTT CGACTCTCCA
    TGGACCACCA GGGCCCTATC CGAGGTCTCC GGGGAGAACC AGAAGGGCCC AGCAGCCACC
    GAACGGGCTG CGGCCACCAT AGTGGCCATG GCAGGGACCG GGCCGGGGAA GGCAGGGGAG
    GGTGAGAAGG AGAAAGTGCT GGCGGAACCG GAGAAGGTGG TGACCGAGCC TCCAGTTGTC
    ACCCTGTTGG ACAAGAAGGA GGGTGAGCAA GCCAAAGCCC TGTTTGAGAA GGTGAAGAAG
    TTCCGCATGC ACGTGGAAGA GGGCGACATC CTGTACACCA TGTACATCCG ACAGACGGTG
    CTGAAAGTGT GTAAGTTCCT GGCCATCCTG GTCTACAACC TGGTCTATGT GGAGAAGATC
    AGTTTCCTGG TGGCCTGTAG GGTGGAGACG TCAGAGGTCA CGGGCTACGC CAGCTTCTGC
    TGCAACCACA CCAAGGCCCA CCTCTTCTCC AAGCTGGCCT TCTGTTACAT CTCCTTTGTG
    TGCATCTACG GACTTACCTG CATCTACACG CTCTACTGGC TCTTCCACCG GCCCCTCAAG
    GAGTACTCCT TCCGTTCCGT GCGGGAGGAG ACTGGCATGG GGGACATTCC TGACGTCAAG
    AATGACTTCG CCTTCATGCT GCACCTCATC GATCAGTACG ACTCCCTCTA CTCCAAGCGC
    TTCGCCGTCT TCCTGTCCGA GGTCAGCGAA AGCCGTCTAA AGCAGCTCAA TCTCAACCAC
    GAGTGGACGC CCGAGAAGCT TCGACAGAAG CTGCAGCGCA ATGCCGCGGG CCGGCTGGAG
    CTGGCCCTCT GCATGCTGCC GGGTCTGCCC GACACCGTCT TTGAGCTCAG TGAGGTGGAG
    TCACTCAGGC TGGAGGCCAT CTGCGATATC ACCTTCCCCC CGGGGCTGTC ACAGCTGGTG
    CACTTGCAGG AGCTCAGCTT GCTCCACTCG CCCGCCAGGC TACCCTTCTC CTTGCAGGTC
    TTCCTGCGGG ACCACCTGAA GGTGATGCGC GTCAAATGCG AGGAGCTCCG CGAGGTGCCG
    CTTTGGGTGT TTGGGCTGCG GGGCTTGGAG GAGCTGCACC TGGAGGGGCT TTTCCCCCAG
    GAGCTAGCTC GGGCAGCCAC CCTGGAGAGC CTCCGGGAGC TGAAGCAGCT CAAGGTGTTG
    TCCCTCCGGA GCAACGCCGG GAAGGTGCCA GCCAGTGTGA CCGACGTTGC TGGCCACCTG
    CAGAGGCTCA GCCTGCACAA CGATGGGGCC CGTCTGGTTG CCCTGAACAG CCTCAAGAAG
    CTGGCGGCAT TGCGGGAGCT GGAGCTGGTG GCCTGCGGGC TGGAGCGCAT CCCCCATGCA
    GTGTTCAGCC TGGGTGCGCT GCAGGAACTT GACCTCAAGG ACAACCACCT GCGCTCCATC
    GAGGAAATCC TCAGCTTCCA GCACTGCCGG AAGCTGGTCA CGCTCAGGCT GTGGCACAAC
    CAGATCGCCT ACGTCCCTGA GCACGTGCGG AAGCTCAGGA GCCTGGAGCA GCTCTACCTC
    AGCTACAACA AGCTGGAGAC CCTGCCCTCC CAGCTCGGCC TGTGCTCAGG CCTCCGTCTG
    CTGGATGTGT CCCACAATGG GCTACACTCC CTGCCACCCG AGGTGGGCCT CCTGCAGAAC
    CTACAGCACC TGGCCCTCTC CTACAATGCC CTGGAGGCCC TGCCCGAAGA GCTCTTCTTC
    TGCCGCAAGC TGCGGACGTT GCTTCTGGGC GACAACCAGC TGAGCCAGCT CTCGCCCCAC
    GTGGGTGCCC TCAGAGCCCT CAGCCGCCTG GAGCTCAAAG GCAACCGCTT AGAGGCGCTG
    CCAGAAGAAC TTGGCAACTG TGGGGGGCTC AAGAAGGCGG GGCTCCTGGT GGAAGACACG
    CTTTACCAGG GTCTGCCGGC AGAAGTGCGG GACAAGATGG AGGAGGAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu00041
    Clone ID Related Accession (Same CDS sequence) NM_001268284.2 , NM_025061.6 , NM_025061.5 , XM_011528319.2 , XM_011528319.1
    Accession Version NM_025061.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2391bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-02
    Organism Homo sapiens(human)
    Product volume-regulated anion channel subunit LRRC8E isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK091134.1, KF459574.1, BX538180.1 and CB049178.1. On Jan 17, 2014 this sequence version replaced NM_025061.4. Transcript Variant: This variant (1) encodes the longer isoform (1). Variants 1 and 2 encode the same protein (isoform 1). ##Evidence-Data-START## Transcript exon combination :: BC070089.1, BX538180.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATCCCAG TGGCCGAGTT CAAGCAGTTC ACGGAACAGC AGCCTGCGTT CAAGGTGCTC 
    AAACCCTGGT GGGACGTGCT GGCCGAGTAC CTCACCGTGG CCATGCTCAT GATTGGGGTC
    TTTGGCTGCA CCCTCCAGGT GACACAGGAC AAGATCATCT GTCTACCCAA TCATGAGCTC
    CAGGAGAACT TATCAGAGGC CCCGTGCCAG CAATTGCTGC CTCGGGGGAT CCCTGAGCAG
    ATTGGGGCCC TGCAGGAGGT TAAAGGCCTT AAGAACAATT TGGACCTGCA GCAATACAGC
    TTTATTAACC AGCTGTGTTA TGAGACGGCC CTGCACTGGT ATGCCAAGTA CTTCCCTTAC
    CTCGTGGTCA TTCACACACT CATCTTCATG GTCTGCACCA GTTTCTGGTT CAAGTTCCCT
    GGCACCAGCT CCAAGATTGA ACACTTCATC TCCATCCTGG GCAAGTGTTT CGACTCTCCA
    TGGACCACCA GGGCCCTATC CGAGGTCTCC GGGGAGAACC AGAAGGGCCC AGCAGCCACC
    GAACGGGCTG CGGCCACCAT AGTGGCCATG GCAGGGACCG GGCCGGGGAA GGCAGGGGAG
    GGTGAGAAGG AGAAAGTGCT GGCGGAACCG GAGAAGGTGG TGACCGAGCC TCCAGTTGTC
    ACCCTGTTGG ACAAGAAGGA GGGTGAGCAA GCCAAAGCCC TGTTTGAGAA GGTGAAGAAG
    TTCCGCATGC ACGTGGAAGA GGGCGACATC CTGTACACCA TGTACATCCG ACAGACGGTG
    CTGAAAGTGT GTAAGTTCCT GGCCATCCTG GTCTACAACC TGGTCTATGT GGAGAAGATC
    AGTTTCCTGG TGGCCTGTAG GGTGGAGACG TCAGAGGTCA CGGGCTACGC CAGCTTCTGC
    TGCAACCACA CCAAGGCCCA CCTCTTCTCC AAGCTGGCCT TCTGTTACAT CTCCTTTGTG
    TGCATCTACG GACTTACCTG CATCTACACG CTCTACTGGC TCTTCCACCG GCCCCTCAAG
    GAGTACTCCT TCCGTTCCGT GCGGGAGGAG ACTGGCATGG GGGACATTCC TGACGTCAAG
    AATGACTTCG CCTTCATGCT GCACCTCATC GATCAGTACG ACTCCCTCTA CTCCAAGCGC
    TTCGCCGTCT TCCTGTCCGA GGTCAGCGAA AGCCGTCTAA AGCAGCTCAA TCTCAACCAC
    GAGTGGACGC CCGAGAAGCT TCGACAGAAG CTGCAGCGCA ATGCCGCGGG CCGGCTGGAG
    CTGGCCCTCT GCATGCTGCC GGGTCTGCCC GACACCGTCT TTGAGCTCAG TGAGGTGGAG
    TCACTCAGGC TGGAGGCCAT CTGCGATATC ACCTTCCCCC CGGGGCTGTC ACAGCTGGTG
    CACTTGCAGG AGCTCAGCTT GCTCCACTCG CCCGCCAGGC TACCCTTCTC CTTGCAGGTC
    TTCCTGCGGG ACCACCTGAA GGTGATGCGC GTCAAATGCG AGGAGCTCCG CGAGGTGCCG
    CTTTGGGTGT TTGGGCTGCG GGGCTTGGAG GAGCTGCACC TGGAGGGGCT TTTCCCCCAG
    GAGCTAGCTC GGGCAGCCAC CCTGGAGAGC CTCCGGGAGC TGAAGCAGCT CAAGGTGTTG
    TCCCTCCGGA GCAACGCCGG GAAGGTGCCA GCCAGTGTGA CCGACGTTGC TGGCCACCTG
    CAGAGGCTCA GCCTGCACAA CGATGGGGCC CGTCTGGTTG CCCTGAACAG CCTCAAGAAG
    CTGGCGGCAT TGCGGGAGCT GGAGCTGGTG GCCTGCGGGC TGGAGCGCAT CCCCCATGCA
    GTGTTCAGCC TGGGTGCGCT GCAGGAACTT GACCTCAAGG ACAACCACCT GCGCTCCATC
    GAGGAAATCC TCAGCTTCCA GCACTGCCGG AAGCTGGTCA CGCTCAGGCT GTGGCACAAC
    CAGATCGCCT ACGTCCCTGA GCACGTGCGG AAGCTCAGGA GCCTGGAGCA GCTCTACCTC
    AGCTACAACA AGCTGGAGAC CCTGCCCTCC CAGCTCGGCC TGTGCTCAGG CCTCCGTCTG
    CTGGATGTGT CCCACAATGG GCTACACTCC CTGCCACCCG AGGTGGGCCT CCTGCAGAAC
    CTACAGCACC TGGCCCTCTC CTACAATGCC CTGGAGGCCC TGCCCGAAGA GCTCTTCTTC
    TGCCGCAAGC TGCGGACGTT GCTTCTGGGC GACAACCAGC TGAGCCAGCT CTCGCCCCAC
    GTGGGTGCCC TCAGAGCCCT CAGCCGCCTG GAGCTCAAAG GCAACCGCTT AGAGGCGCTG
    CCAGAAGAAC TTGGCAACTG TGGGGGGCTC AAGAAGGCGG GGCTCCTGGT GGAAGACACG
    CTTTACCAGG GTCTGCCGGC AGAAGTGCGG GACAAGATGG AGGAGGAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_079337.2
    CDS102..2492
    Translation

    Target ORF information:

    RefSeq Version NM_025061.5
    Organism Homo sapiens(human)
    Definition Homo sapiens leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_025061.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATCCCAG TGGCCGAGTT CAAGCAGTTC ACGGAACAGC AGCCTGCGTT CAAGGTGCTC 
    AAACCCTGGT GGGACGTGCT GGCCGAGTAC CTCACCGTGG CCATGCTCAT GATTGGGGTC
    TTTGGCTGCA CCCTCCAGGT GACACAGGAC AAGATCATCT GTCTACCCAA TCATGAGCTC
    CAGGAGAACT TATCAGAGGC CCCGTGCCAG CAATTGCTGC CTCGGGGGAT CCCTGAGCAG
    ATTGGGGCCC TGCAGGAGGT TAAAGGCCTT AAGAACAATT TGGACCTGCA GCAATACAGC
    TTTATTAACC AGCTGTGTTA TGAGACGGCC CTGCACTGGT ATGCCAAGTA CTTCCCTTAC
    CTCGTGGTCA TTCACACACT CATCTTCATG GTCTGCACCA GTTTCTGGTT CAAGTTCCCT
    GGCACCAGCT CCAAGATTGA ACACTTCATC TCCATCCTGG GCAAGTGTTT CGACTCTCCA
    TGGACCACCA GGGCCCTATC CGAGGTCTCC GGGGAGAACC AGAAGGGCCC AGCAGCCACC
    GAACGGGCTG CGGCCACCAT AGTGGCCATG GCAGGGACCG GGCCGGGGAA GGCAGGGGAG
    GGTGAGAAGG AGAAAGTGCT GGCGGAACCG GAGAAGGTGG TGACCGAGCC TCCAGTTGTC
    ACCCTGTTGG ACAAGAAGGA GGGTGAGCAA GCCAAAGCCC TGTTTGAGAA GGTGAAGAAG
    TTCCGCATGC ACGTGGAAGA GGGCGACATC CTGTACACCA TGTACATCCG ACAGACGGTG
    CTGAAAGTGT GTAAGTTCCT GGCCATCCTG GTCTACAACC TGGTCTATGT GGAGAAGATC
    AGTTTCCTGG TGGCCTGTAG GGTGGAGACG TCAGAGGTCA CGGGCTACGC CAGCTTCTGC
    TGCAACCACA CCAAGGCCCA CCTCTTCTCC AAGCTGGCCT TCTGTTACAT CTCCTTTGTG
    TGCATCTACG GACTTACCTG CATCTACACG CTCTACTGGC TCTTCCACCG GCCCCTCAAG
    GAGTACTCCT TCCGTTCCGT GCGGGAGGAG ACTGGCATGG GGGACATTCC TGACGTCAAG
    AATGACTTCG CCTTCATGCT GCACCTCATC GATCAGTACG ACTCCCTCTA CTCCAAGCGC
    TTCGCCGTCT TCCTGTCCGA GGTCAGCGAA AGCCGTCTAA AGCAGCTCAA TCTCAACCAC
    GAGTGGACGC CCGAGAAGCT TCGACAGAAG CTGCAGCGCA ATGCCGCGGG CCGGCTGGAG
    CTGGCCCTCT GCATGCTGCC GGGTCTGCCC GACACCGTCT TTGAGCTCAG TGAGGTGGAG
    TCACTCAGGC TGGAGGCCAT CTGCGATATC ACCTTCCCCC CGGGGCTGTC ACAGCTGGTG
    CACTTGCAGG AGCTCAGCTT GCTCCACTCG CCCGCCAGGC TACCCTTCTC CTTGCAGGTC
    TTCCTGCGGG ACCACCTGAA GGTGATGCGC GTCAAATGCG AGGAGCTCCG CGAGGTGCCG
    CTTTGGGTGT TTGGGCTGCG GGGCTTGGAG GAGCTGCACC TGGAGGGGCT TTTCCCCCAG
    GAGCTAGCTC GGGCAGCCAC CCTGGAGAGC CTCCGGGAGC TGAAGCAGCT CAAGGTGTTG
    TCCCTCCGGA GCAACGCCGG GAAGGTGCCA GCCAGTGTGA CCGACGTTGC TGGCCACCTG
    CAGAGGCTCA GCCTGCACAA CGATGGGGCC CGTCTGGTTG CCCTGAACAG CCTCAAGAAG
    CTGGCGGCAT TGCGGGAGCT GGAGCTGGTG GCCTGCGGGC TGGAGCGCAT CCCCCATGCA
    GTGTTCAGCC TGGGTGCGCT GCAGGAACTT GACCTCAAGG ACAACCACCT GCGCTCCATC
    GAGGAAATCC TCAGCTTCCA GCACTGCCGG AAGCTGGTCA CGCTCAGGCT GTGGCACAAC
    CAGATCGCCT ACGTCCCTGA GCACGTGCGG AAGCTCAGGA GCCTGGAGCA GCTCTACCTC
    AGCTACAACA AGCTGGAGAC CCTGCCCTCC CAGCTCGGCC TGTGCTCAGG CCTCCGTCTG
    CTGGATGTGT CCCACAATGG GCTACACTCC CTGCCACCCG AGGTGGGCCT CCTGCAGAAC
    CTACAGCACC TGGCCCTCTC CTACAATGCC CTGGAGGCCC TGCCCGAAGA GCTCTTCTTC
    TGCCGCAAGC TGCGGACGTT GCTTCTGGGC GACAACCAGC TGAGCCAGCT CTCGCCCCAC
    GTGGGTGCCC TCAGAGCCCT CAGCCGCCTG GAGCTCAAAG GCAACCGCTT AGAGGCGCTG
    CCAGAAGAAC TTGGCAACTG TGGGGGGCTC AAGAAGGCGG GGCTCCTGGT GGAAGACACG
    CTTTACCAGG GTCTGCCGGC AGAAGTGCGG GACAAGATGG AGGAGGAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu29088
    Clone ID Related Accession (Same CDS sequence) NM_001268285.3 , NM_001268285.2
    Accession Version NM_001268285.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2004bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-05-30
    Organism Homo sapiens(human)
    Product volume-regulated anion channel subunit LRRC8E isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK091134.1, DA447942.1, KF459574.1, BX538180.1, AC010336.8 and CB049178.1. On May 31, 2019 this sequence version replaced NM_001268285.2. Transcript Variant: This variant (3) lacks an exon in the 5' coding region, and uses a downstream in-frame start codon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR6380202.129487.3, SRR6380202.129487.11 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144120, SAMEA2144333 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGTCTGCA CCAGTTTCTG GTTCAAGTTC CCTGGCACCA GCTCCAAGAT TGAACACTTC 
    ATCTCCATCC TGGGCAAGTG TTTCGACTCT CCATGGACCA CCAGGGCCCT ATCCGAGGTC
    TCCGGGGAGA ACCAGAAGGG CCCAGCAGCC ACCGAACGGG CTGCGGCCAC CATAGTGGCC
    ATGGCAGGGA CCGGGCCGGG GAAGGCAGGG GAGGGTGAGA AGGAGAAAGT GCTGGCGGAA
    CCGGAGAAGG TGGTGACCGA GCCTCCAGTT GTCACCCTGT TGGACAAGAA GGAGGGTGAG
    CAAGCCAAAG CCCTGTTTGA GAAGGTGAAG AAGTTCCGCA TGCACGTGGA AGAGGGCGAC
    ATCCTGTACA CCATGTACAT CCGACAGACG GTGCTGAAAG TGTGTAAGTT CCTGGCCATC
    CTGGTCTACA ACCTGGTCTA TGTGGAGAAG ATCAGTTTCC TGGTGGCCTG TAGGGTGGAG
    ACGTCAGAGG TCACGGGCTA CGCCAGCTTC TGCTGCAACC ACACCAAGGC CCACCTCTTC
    TCCAAGCTGG CCTTCTGTTA CATCTCCTTT GTGTGCATCT ACGGACTTAC CTGCATCTAC
    ACGCTCTACT GGCTCTTCCA CCGGCCCCTC AAGGAGTACT CCTTCCGTTC CGTGCGGGAG
    GAGACTGGCA TGGGGGACAT TCCTGACGTC AAGAATGACT TCGCCTTCAT GCTGCACCTC
    ATCGATCAGT ACGACTCCCT CTACTCCAAG CGCTTCGCCG TCTTCCTGTC CGAGGTCAGC
    GAAAGCCGTC TAAAGCAGCT CAATCTCAAC CACGAGTGGA CGCCCGAGAA GCTTCGACAG
    AAGCTGCAGC GCAATGCCGC GGGCCGGCTG GAGCTGGCCC TCTGCATGCT GCCGGGTCTG
    CCCGACACCG TCTTTGAGCT CAGTGAGGTG GAGTCACTCA GGCTGGAGGC CATCTGCGAT
    ATCACCTTCC CCCCGGGGCT GTCACAGCTG GTGCACTTGC AGGAGCTCAG CTTGCTCCAC
    TCGCCCGCCA GGCTACCCTT CTCCTTGCAG GTCTTCCTGC GGGACCACCT GAAGGTGATG
    CGCGTCAAAT GCGAGGAGCT CCGCGAGGTG CCGCTTTGGG TGTTTGGGCT GCGGGGCTTG
    GAGGAGCTGC ACCTGGAGGG GCTTTTCCCC CAGGAGCTAG CTCGGGCAGC CACCCTGGAG
    AGCCTCCGGG AGCTGAAGCA GCTCAAGGTG TTGTCCCTCC GGAGCAACGC CGGGAAGGTG
    CCAGCCAGTG TGACCGACGT TGCTGGCCAC CTGCAGAGGC TCAGCCTGCA CAACGATGGG
    GCCCGTCTGG TTGCCCTGAA CAGCCTCAAG AAGCTGGCGG CATTGCGGGA GCTGGAGCTG
    GTGGCCTGCG GGCTGGAGCG CATCCCCCAT GCAGTGTTCA GCCTGGGTGC GCTGCAGGAA
    CTTGACCTCA AGGACAACCA CCTGCGCTCC ATCGAGGAAA TCCTCAGCTT CCAGCACTGC
    CGGAAGCTGG TCACGCTCAG GCTGTGGCAC AACCAGATCG CCTACGTCCC TGAGCACGTG
    CGGAAGCTCA GGAGCCTGGA GCAGCTCTAC CTCAGCTACA ACAAGCTGGA GACCCTGCCC
    TCCCAGCTCG GCCTGTGCTC AGGCCTCCGT CTGCTGGATG TGTCCCACAA TGGGCTACAC
    TCCCTGCCAC CCGAGGTGGG CCTCCTGCAG AACCTACAGC ACCTGGCCCT CTCCTACAAT
    GCCCTGGAGG CCCTGCCCGA AGAGCTCTTC TTCTGCCGCA AGCTGCGGAC GTTGCTTCTG
    GGCGACAACC AGCTGAGCCA GCTCTCGCCC CACGTGGGTG CCCTCAGAGC CCTCAGCCGC
    CTGGAGCTCA AAGGCAACCG CTTAGAGGCG CTGCCAGAAG AACTTGGCAA CTGTGGGGGG
    CTCAAGAAGG CGGGGCTCCT GGTGGAAGAC ACGCTTTACC AGGGTCTGCC GGCAGAAGTG
    CGGGACAAGA TGGAGGAGGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255214.1
    CDS341..2344
    Translation

    Target ORF information:

    RefSeq Version NM_001268285.3
    Organism Homo sapiens(human)
    Definition Homo sapiens leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268285.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGTCTGCA CCAGTTTCTG GTTCAAGTTC CCTGGCACCA GCTCCAAGAT TGAACACTTC 
    ATCTCCATCC TGGGCAAGTG TTTCGACTCT CCATGGACCA CCAGGGCCCT ATCCGAGGTC
    TCCGGGGAGA ACCAGAAGGG CCCAGCAGCC ACCGAACGGG CTGCGGCCAC CATAGTGGCC
    ATGGCAGGGA CCGGGCCGGG GAAGGCAGGG GAGGGTGAGA AGGAGAAAGT GCTGGCGGAA
    CCGGAGAAGG TGGTGACCGA GCCTCCAGTT GTCACCCTGT TGGACAAGAA GGAGGGTGAG
    CAAGCCAAAG CCCTGTTTGA GAAGGTGAAG AAGTTCCGCA TGCACGTGGA AGAGGGCGAC
    ATCCTGTACA CCATGTACAT CCGACAGACG GTGCTGAAAG TGTGTAAGTT CCTGGCCATC
    CTGGTCTACA ACCTGGTCTA TGTGGAGAAG ATCAGTTTCC TGGTGGCCTG TAGGGTGGAG
    ACGTCAGAGG TCACGGGCTA CGCCAGCTTC TGCTGCAACC ACACCAAGGC CCACCTCTTC
    TCCAAGCTGG CCTTCTGTTA CATCTCCTTT GTGTGCATCT ACGGACTTAC CTGCATCTAC
    ACGCTCTACT GGCTCTTCCA CCGGCCCCTC AAGGAGTACT CCTTCCGTTC CGTGCGGGAG
    GAGACTGGCA TGGGGGACAT TCCTGACGTC AAGAATGACT TCGCCTTCAT GCTGCACCTC
    ATCGATCAGT ACGACTCCCT CTACTCCAAG CGCTTCGCCG TCTTCCTGTC CGAGGTCAGC
    GAAAGCCGTC TAAAGCAGCT CAATCTCAAC CACGAGTGGA CGCCCGAGAA GCTTCGACAG
    AAGCTGCAGC GCAATGCCGC GGGCCGGCTG GAGCTGGCCC TCTGCATGCT GCCGGGTCTG
    CCCGACACCG TCTTTGAGCT CAGTGAGGTG GAGTCACTCA GGCTGGAGGC CATCTGCGAT
    ATCACCTTCC CCCCGGGGCT GTCACAGCTG GTGCACTTGC AGGAGCTCAG CTTGCTCCAC
    TCGCCCGCCA GGCTACCCTT CTCCTTGCAG GTCTTCCTGC GGGACCACCT GAAGGTGATG
    CGCGTCAAAT GCGAGGAGCT CCGCGAGGTG CCGCTTTGGG TGTTTGGGCT GCGGGGCTTG
    GAGGAGCTGC ACCTGGAGGG GCTTTTCCCC CAGGAGCTAG CTCGGGCAGC CACCCTGGAG
    AGCCTCCGGG AGCTGAAGCA GCTCAAGGTG TTGTCCCTCC GGAGCAACGC CGGGAAGGTG
    CCAGCCAGTG TGACCGACGT TGCTGGCCAC CTGCAGAGGC TCAGCCTGCA CAACGATGGG
    GCCCGTCTGG TTGCCCTGAA CAGCCTCAAG AAGCTGGCGG CATTGCGGGA GCTGGAGCTG
    GTGGCCTGCG GGCTGGAGCG CATCCCCCAT GCAGTGTTCA GCCTGGGTGC GCTGCAGGAA
    CTTGACCTCA AGGACAACCA CCTGCGCTCC ATCGAGGAAA TCCTCAGCTT CCAGCACTGC
    CGGAAGCTGG TCACGCTCAG GCTGTGGCAC AACCAGATCG CCTACGTCCC TGAGCACGTG
    CGGAAGCTCA GGAGCCTGGA GCAGCTCTAC CTCAGCTACA ACAAGCTGGA GACCCTGCCC
    TCCCAGCTCG GCCTGTGCTC AGGCCTCCGT CTGCTGGATG TGTCCCACAA TGGGCTACAC
    TCCCTGCCAC CCGAGGTGGG CCTCCTGCAG AACCTACAGC ACCTGGCCCT CTCCTACAAT
    GCCCTGGAGG CCCTGCCCGA AGAGCTCTTC TTCTGCCGCA AGCTGCGGAC GTTGCTTCTG
    GGCGACAACC AGCTGAGCCA GCTCTCGCCC CACGTGGGTG CCCTCAGAGC CCTCAGCCGC
    CTGGAGCTCA AAGGCAACCG CTTAGAGGCG CTGCCAGAAG AACTTGGCAA CTGTGGGGGG
    CTCAAGAAGG CGGGGCTCCT GGTGGAAGAC ACGCTTTACC AGGGTCTGCC GGCAGAAGTG
    CGGGACAAGA TGGAGGAGGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu29088
    Clone ID Related Accession (Same CDS sequence) NM_001268285.3 , NM_001268285.2
    Accession Version NM_001268285.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2004bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-08-10
    Organism Homo sapiens(human)
    Product volume-regulated anion channel subunit LRRC8E isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK091134.1, DA447942.1, KF459574.1, BX538180.1, AC010336.8 and CB049178.1. On Jan 17, 2014 this sequence version replaced NM_001268285.1. Transcript Variant: This variant (3) lacks an exon in the 5' coding region, and uses a downstream in-frame start codon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: DA447942.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144120, SAMEA2144333 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGTCTGCA CCAGTTTCTG GTTCAAGTTC CCTGGCACCA GCTCCAAGAT TGAACACTTC 
    ATCTCCATCC TGGGCAAGTG TTTCGACTCT CCATGGACCA CCAGGGCCCT ATCCGAGGTC
    TCCGGGGAGA ACCAGAAGGG CCCAGCAGCC ACCGAACGGG CTGCGGCCAC CATAGTGGCC
    ATGGCAGGGA CCGGGCCGGG GAAGGCAGGG GAGGGTGAGA AGGAGAAAGT GCTGGCGGAA
    CCGGAGAAGG TGGTGACCGA GCCTCCAGTT GTCACCCTGT TGGACAAGAA GGAGGGTGAG
    CAAGCCAAAG CCCTGTTTGA GAAGGTGAAG AAGTTCCGCA TGCACGTGGA AGAGGGCGAC
    ATCCTGTACA CCATGTACAT CCGACAGACG GTGCTGAAAG TGTGTAAGTT CCTGGCCATC
    CTGGTCTACA ACCTGGTCTA TGTGGAGAAG ATCAGTTTCC TGGTGGCCTG TAGGGTGGAG
    ACGTCAGAGG TCACGGGCTA CGCCAGCTTC TGCTGCAACC ACACCAAGGC CCACCTCTTC
    TCCAAGCTGG CCTTCTGTTA CATCTCCTTT GTGTGCATCT ACGGACTTAC CTGCATCTAC
    ACGCTCTACT GGCTCTTCCA CCGGCCCCTC AAGGAGTACT CCTTCCGTTC CGTGCGGGAG
    GAGACTGGCA TGGGGGACAT TCCTGACGTC AAGAATGACT TCGCCTTCAT GCTGCACCTC
    ATCGATCAGT ACGACTCCCT CTACTCCAAG CGCTTCGCCG TCTTCCTGTC CGAGGTCAGC
    GAAAGCCGTC TAAAGCAGCT CAATCTCAAC CACGAGTGGA CGCCCGAGAA GCTTCGACAG
    AAGCTGCAGC GCAATGCCGC GGGCCGGCTG GAGCTGGCCC TCTGCATGCT GCCGGGTCTG
    CCCGACACCG TCTTTGAGCT CAGTGAGGTG GAGTCACTCA GGCTGGAGGC CATCTGCGAT
    ATCACCTTCC CCCCGGGGCT GTCACAGCTG GTGCACTTGC AGGAGCTCAG CTTGCTCCAC
    TCGCCCGCCA GGCTACCCTT CTCCTTGCAG GTCTTCCTGC GGGACCACCT GAAGGTGATG
    CGCGTCAAAT GCGAGGAGCT CCGCGAGGTG CCGCTTTGGG TGTTTGGGCT GCGGGGCTTG
    GAGGAGCTGC ACCTGGAGGG GCTTTTCCCC CAGGAGCTAG CTCGGGCAGC CACCCTGGAG
    AGCCTCCGGG AGCTGAAGCA GCTCAAGGTG TTGTCCCTCC GGAGCAACGC CGGGAAGGTG
    CCAGCCAGTG TGACCGACGT TGCTGGCCAC CTGCAGAGGC TCAGCCTGCA CAACGATGGG
    GCCCGTCTGG TTGCCCTGAA CAGCCTCAAG AAGCTGGCGG CATTGCGGGA GCTGGAGCTG
    GTGGCCTGCG GGCTGGAGCG CATCCCCCAT GCAGTGTTCA GCCTGGGTGC GCTGCAGGAA
    CTTGACCTCA AGGACAACCA CCTGCGCTCC ATCGAGGAAA TCCTCAGCTT CCAGCACTGC
    CGGAAGCTGG TCACGCTCAG GCTGTGGCAC AACCAGATCG CCTACGTCCC TGAGCACGTG
    CGGAAGCTCA GGAGCCTGGA GCAGCTCTAC CTCAGCTACA ACAAGCTGGA GACCCTGCCC
    TCCCAGCTCG GCCTGTGCTC AGGCCTCCGT CTGCTGGATG TGTCCCACAA TGGGCTACAC
    TCCCTGCCAC CCGAGGTGGG CCTCCTGCAG AACCTACAGC ACCTGGCCCT CTCCTACAAT
    GCCCTGGAGG CCCTGCCCGA AGAGCTCTTC TTCTGCCGCA AGCTGCGGAC GTTGCTTCTG
    GGCGACAACC AGCTGAGCCA GCTCTCGCCC CACGTGGGTG CCCTCAGAGC CCTCAGCCGC
    CTGGAGCTCA AAGGCAACCG CTTAGAGGCG CTGCCAGAAG AACTTGGCAA CTGTGGGGGG
    CTCAAGAAGG CGGGGCTCCT GGTGGAAGAC ACGCTTTACC AGGGTCTGCC GGCAGAAGTG
    CGGGACAAGA TGGAGGAGGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255214.1
    CDS346..2349
    Translation

    Target ORF information:

    RefSeq Version NM_001268285.2
    Organism Homo sapiens(human)
    Definition Homo sapiens leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268285.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGTCTGCA CCAGTTTCTG GTTCAAGTTC CCTGGCACCA GCTCCAAGAT TGAACACTTC 
    ATCTCCATCC TGGGCAAGTG TTTCGACTCT CCATGGACCA CCAGGGCCCT ATCCGAGGTC
    TCCGGGGAGA ACCAGAAGGG CCCAGCAGCC ACCGAACGGG CTGCGGCCAC CATAGTGGCC
    ATGGCAGGGA CCGGGCCGGG GAAGGCAGGG GAGGGTGAGA AGGAGAAAGT GCTGGCGGAA
    CCGGAGAAGG TGGTGACCGA GCCTCCAGTT GTCACCCTGT TGGACAAGAA GGAGGGTGAG
    CAAGCCAAAG CCCTGTTTGA GAAGGTGAAG AAGTTCCGCA TGCACGTGGA AGAGGGCGAC
    ATCCTGTACA CCATGTACAT CCGACAGACG GTGCTGAAAG TGTGTAAGTT CCTGGCCATC
    CTGGTCTACA ACCTGGTCTA TGTGGAGAAG ATCAGTTTCC TGGTGGCCTG TAGGGTGGAG
    ACGTCAGAGG TCACGGGCTA CGCCAGCTTC TGCTGCAACC ACACCAAGGC CCACCTCTTC
    TCCAAGCTGG CCTTCTGTTA CATCTCCTTT GTGTGCATCT ACGGACTTAC CTGCATCTAC
    ACGCTCTACT GGCTCTTCCA CCGGCCCCTC AAGGAGTACT CCTTCCGTTC CGTGCGGGAG
    GAGACTGGCA TGGGGGACAT TCCTGACGTC AAGAATGACT TCGCCTTCAT GCTGCACCTC
    ATCGATCAGT ACGACTCCCT CTACTCCAAG CGCTTCGCCG TCTTCCTGTC CGAGGTCAGC
    GAAAGCCGTC TAAAGCAGCT CAATCTCAAC CACGAGTGGA CGCCCGAGAA GCTTCGACAG
    AAGCTGCAGC GCAATGCCGC GGGCCGGCTG GAGCTGGCCC TCTGCATGCT GCCGGGTCTG
    CCCGACACCG TCTTTGAGCT CAGTGAGGTG GAGTCACTCA GGCTGGAGGC CATCTGCGAT
    ATCACCTTCC CCCCGGGGCT GTCACAGCTG GTGCACTTGC AGGAGCTCAG CTTGCTCCAC
    TCGCCCGCCA GGCTACCCTT CTCCTTGCAG GTCTTCCTGC GGGACCACCT GAAGGTGATG
    CGCGTCAAAT GCGAGGAGCT CCGCGAGGTG CCGCTTTGGG TGTTTGGGCT GCGGGGCTTG
    GAGGAGCTGC ACCTGGAGGG GCTTTTCCCC CAGGAGCTAG CTCGGGCAGC CACCCTGGAG
    AGCCTCCGGG AGCTGAAGCA GCTCAAGGTG TTGTCCCTCC GGAGCAACGC CGGGAAGGTG
    CCAGCCAGTG TGACCGACGT TGCTGGCCAC CTGCAGAGGC TCAGCCTGCA CAACGATGGG
    GCCCGTCTGG TTGCCCTGAA CAGCCTCAAG AAGCTGGCGG CATTGCGGGA GCTGGAGCTG
    GTGGCCTGCG GGCTGGAGCG CATCCCCCAT GCAGTGTTCA GCCTGGGTGC GCTGCAGGAA
    CTTGACCTCA AGGACAACCA CCTGCGCTCC ATCGAGGAAA TCCTCAGCTT CCAGCACTGC
    CGGAAGCTGG TCACGCTCAG GCTGTGGCAC AACCAGATCG CCTACGTCCC TGAGCACGTG
    CGGAAGCTCA GGAGCCTGGA GCAGCTCTAC CTCAGCTACA ACAAGCTGGA GACCCTGCCC
    TCCCAGCTCG GCCTGTGCTC AGGCCTCCGT CTGCTGGATG TGTCCCACAA TGGGCTACAC
    TCCCTGCCAC CCGAGGTGGG CCTCCTGCAG AACCTACAGC ACCTGGCCCT CTCCTACAAT
    GCCCTGGAGG CCCTGCCCGA AGAGCTCTTC TTCTGCCGCA AGCTGCGGAC GTTGCTTCTG
    GGCGACAACC AGCTGAGCCA GCTCTCGCCC CACGTGGGTG CCCTCAGAGC CCTCAGCCGC
    CTGGAGCTCA AAGGCAACCG CTTAGAGGCG CTGCCAGAAG AACTTGGCAA CTGTGGGGGG
    CTCAAGAAGG CGGGGCTCCT GGTGGAAGAC ACGCTTTACC AGGGTCTGCC GGCAGAAGTG
    CGGGACAAGA TGGAGGAGGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu00041
    Clone ID Related Accession (Same CDS sequence) NM_001268284.2 , NM_025061.6 , NM_025061.5 , XM_011528319.2 , XM_011528319.1
    Accession Version XM_011528319.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2391bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product volume-regulated anion channel subunit LRRC8E isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011528319.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATCCCAG TGGCCGAGTT CAAGCAGTTC ACGGAACAGC AGCCTGCGTT CAAGGTGCTC 
    AAACCCTGGT GGGACGTGCT GGCCGAGTAC CTCACCGTGG CCATGCTCAT GATTGGGGTC
    TTTGGCTGCA CCCTCCAGGT GACACAGGAC AAGATCATCT GTCTACCCAA TCATGAGCTC
    CAGGAGAACT TATCAGAGGC CCCGTGCCAG CAATTGCTGC CTCGGGGGAT CCCTGAGCAG
    ATTGGGGCCC TGCAGGAGGT TAAAGGCCTT AAGAACAATT TGGACCTGCA GCAATACAGC
    TTTATTAACC AGCTGTGTTA TGAGACGGCC CTGCACTGGT ATGCCAAGTA CTTCCCTTAC
    CTCGTGGTCA TTCACACACT CATCTTCATG GTCTGCACCA GTTTCTGGTT CAAGTTCCCT
    GGCACCAGCT CCAAGATTGA ACACTTCATC TCCATCCTGG GCAAGTGTTT CGACTCTCCA
    TGGACCACCA GGGCCCTATC CGAGGTCTCC GGGGAGAACC AGAAGGGCCC AGCAGCCACC
    GAACGGGCTG CGGCCACCAT AGTGGCCATG GCAGGGACCG GGCCGGGGAA GGCAGGGGAG
    GGTGAGAAGG AGAAAGTGCT GGCGGAACCG GAGAAGGTGG TGACCGAGCC TCCAGTTGTC
    ACCCTGTTGG ACAAGAAGGA GGGTGAGCAA GCCAAAGCCC TGTTTGAGAA GGTGAAGAAG
    TTCCGCATGC ACGTGGAAGA GGGCGACATC CTGTACACCA TGTACATCCG ACAGACGGTG
    CTGAAAGTGT GTAAGTTCCT GGCCATCCTG GTCTACAACC TGGTCTATGT GGAGAAGATC
    AGTTTCCTGG TGGCCTGTAG GGTGGAGACG TCAGAGGTCA CGGGCTACGC CAGCTTCTGC
    TGCAACCACA CCAAGGCCCA CCTCTTCTCC AAGCTGGCCT TCTGTTACAT CTCCTTTGTG
    TGCATCTACG GACTTACCTG CATCTACACG CTCTACTGGC TCTTCCACCG GCCCCTCAAG
    GAGTACTCCT TCCGTTCCGT GCGGGAGGAG ACTGGCATGG GGGACATTCC TGACGTCAAG
    AATGACTTCG CCTTCATGCT GCACCTCATC GATCAGTACG ACTCCCTCTA CTCCAAGCGC
    TTCGCCGTCT TCCTGTCCGA GGTCAGCGAA AGCCGTCTAA AGCAGCTCAA TCTCAACCAC
    GAGTGGACGC CCGAGAAGCT TCGACAGAAG CTGCAGCGCA ATGCCGCGGG CCGGCTGGAG
    CTGGCCCTCT GCATGCTGCC GGGTCTGCCC GACACCGTCT TTGAGCTCAG TGAGGTGGAG
    TCACTCAGGC TGGAGGCCAT CTGCGATATC ACCTTCCCCC CGGGGCTGTC ACAGCTGGTG
    CACTTGCAGG AGCTCAGCTT GCTCCACTCG CCCGCCAGGC TACCCTTCTC CTTGCAGGTC
    TTCCTGCGGG ACCACCTGAA GGTGATGCGC GTCAAATGCG AGGAGCTCCG CGAGGTGCCG
    CTTTGGGTGT TTGGGCTGCG GGGCTTGGAG GAGCTGCACC TGGAGGGGCT TTTCCCCCAG
    GAGCTAGCTC GGGCAGCCAC CCTGGAGAGC CTCCGGGAGC TGAAGCAGCT CAAGGTGTTG
    TCCCTCCGGA GCAACGCCGG GAAGGTGCCA GCCAGTGTGA CCGACGTTGC TGGCCACCTG
    CAGAGGCTCA GCCTGCACAA CGATGGGGCC CGTCTGGTTG CCCTGAACAG CCTCAAGAAG
    CTGGCGGCAT TGCGGGAGCT GGAGCTGGTG GCCTGCGGGC TGGAGCGCAT CCCCCATGCA
    GTGTTCAGCC TGGGTGCGCT GCAGGAACTT GACCTCAAGG ACAACCACCT GCGCTCCATC
    GAGGAAATCC TCAGCTTCCA GCACTGCCGG AAGCTGGTCA CGCTCAGGCT GTGGCACAAC
    CAGATCGCCT ACGTCCCTGA GCACGTGCGG AAGCTCAGGA GCCTGGAGCA GCTCTACCTC
    AGCTACAACA AGCTGGAGAC CCTGCCCTCC CAGCTCGGCC TGTGCTCAGG CCTCCGTCTG
    CTGGATGTGT CCCACAATGG GCTACACTCC CTGCCACCCG AGGTGGGCCT CCTGCAGAAC
    CTACAGCACC TGGCCCTCTC CTACAATGCC CTGGAGGCCC TGCCCGAAGA GCTCTTCTTC
    TGCCGCAAGC TGCGGACGTT GCTTCTGGGC GACAACCAGC TGAGCCAGCT CTCGCCCCAC
    GTGGGTGCCC TCAGAGCCCT CAGCCGCCTG GAGCTCAAAG GCAACCGCTT AGAGGCGCTG
    CCAGAAGAAC TTGGCAACTG TGGGGGGCTC AAGAAGGCGG GGCTCCTGGT GGAAGACACG
    CTTTACCAGG GTCTGCCGGC AGAAGTGCGG GACAAGATGG AGGAGGAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011526621.1
    CDS830..3220
    Translation

    Target ORF information:

    RefSeq Version XM_011528319.2
    Organism Homo sapiens(human)
    Definition Homo sapiens leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011528319.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATCCCAG TGGCCGAGTT CAAGCAGTTC ACGGAACAGC AGCCTGCGTT CAAGGTGCTC 
    AAACCCTGGT GGGACGTGCT GGCCGAGTAC CTCACCGTGG CCATGCTCAT GATTGGGGTC
    TTTGGCTGCA CCCTCCAGGT GACACAGGAC AAGATCATCT GTCTACCCAA TCATGAGCTC
    CAGGAGAACT TATCAGAGGC CCCGTGCCAG CAATTGCTGC CTCGGGGGAT CCCTGAGCAG
    ATTGGGGCCC TGCAGGAGGT TAAAGGCCTT AAGAACAATT TGGACCTGCA GCAATACAGC
    TTTATTAACC AGCTGTGTTA TGAGACGGCC CTGCACTGGT ATGCCAAGTA CTTCCCTTAC
    CTCGTGGTCA TTCACACACT CATCTTCATG GTCTGCACCA GTTTCTGGTT CAAGTTCCCT
    GGCACCAGCT CCAAGATTGA ACACTTCATC TCCATCCTGG GCAAGTGTTT CGACTCTCCA
    TGGACCACCA GGGCCCTATC CGAGGTCTCC GGGGAGAACC AGAAGGGCCC AGCAGCCACC
    GAACGGGCTG CGGCCACCAT AGTGGCCATG GCAGGGACCG GGCCGGGGAA GGCAGGGGAG
    GGTGAGAAGG AGAAAGTGCT GGCGGAACCG GAGAAGGTGG TGACCGAGCC TCCAGTTGTC
    ACCCTGTTGG ACAAGAAGGA GGGTGAGCAA GCCAAAGCCC TGTTTGAGAA GGTGAAGAAG
    TTCCGCATGC ACGTGGAAGA GGGCGACATC CTGTACACCA TGTACATCCG ACAGACGGTG
    CTGAAAGTGT GTAAGTTCCT GGCCATCCTG GTCTACAACC TGGTCTATGT GGAGAAGATC
    AGTTTCCTGG TGGCCTGTAG GGTGGAGACG TCAGAGGTCA CGGGCTACGC CAGCTTCTGC
    TGCAACCACA CCAAGGCCCA CCTCTTCTCC AAGCTGGCCT TCTGTTACAT CTCCTTTGTG
    TGCATCTACG GACTTACCTG CATCTACACG CTCTACTGGC TCTTCCACCG GCCCCTCAAG
    GAGTACTCCT TCCGTTCCGT GCGGGAGGAG ACTGGCATGG GGGACATTCC TGACGTCAAG
    AATGACTTCG CCTTCATGCT GCACCTCATC GATCAGTACG ACTCCCTCTA CTCCAAGCGC
    TTCGCCGTCT TCCTGTCCGA GGTCAGCGAA AGCCGTCTAA AGCAGCTCAA TCTCAACCAC
    GAGTGGACGC CCGAGAAGCT TCGACAGAAG CTGCAGCGCA ATGCCGCGGG CCGGCTGGAG
    CTGGCCCTCT GCATGCTGCC GGGTCTGCCC GACACCGTCT TTGAGCTCAG TGAGGTGGAG
    TCACTCAGGC TGGAGGCCAT CTGCGATATC ACCTTCCCCC CGGGGCTGTC ACAGCTGGTG
    CACTTGCAGG AGCTCAGCTT GCTCCACTCG CCCGCCAGGC TACCCTTCTC CTTGCAGGTC
    TTCCTGCGGG ACCACCTGAA GGTGATGCGC GTCAAATGCG AGGAGCTCCG CGAGGTGCCG
    CTTTGGGTGT TTGGGCTGCG GGGCTTGGAG GAGCTGCACC TGGAGGGGCT TTTCCCCCAG
    GAGCTAGCTC GGGCAGCCAC CCTGGAGAGC CTCCGGGAGC TGAAGCAGCT CAAGGTGTTG
    TCCCTCCGGA GCAACGCCGG GAAGGTGCCA GCCAGTGTGA CCGACGTTGC TGGCCACCTG
    CAGAGGCTCA GCCTGCACAA CGATGGGGCC CGTCTGGTTG CCCTGAACAG CCTCAAGAAG
    CTGGCGGCAT TGCGGGAGCT GGAGCTGGTG GCCTGCGGGC TGGAGCGCAT CCCCCATGCA
    GTGTTCAGCC TGGGTGCGCT GCAGGAACTT GACCTCAAGG ACAACCACCT GCGCTCCATC
    GAGGAAATCC TCAGCTTCCA GCACTGCCGG AAGCTGGTCA CGCTCAGGCT GTGGCACAAC
    CAGATCGCCT ACGTCCCTGA GCACGTGCGG AAGCTCAGGA GCCTGGAGCA GCTCTACCTC
    AGCTACAACA AGCTGGAGAC CCTGCCCTCC CAGCTCGGCC TGTGCTCAGG CCTCCGTCTG
    CTGGATGTGT CCCACAATGG GCTACACTCC CTGCCACCCG AGGTGGGCCT CCTGCAGAAC
    CTACAGCACC TGGCCCTCTC CTACAATGCC CTGGAGGCCC TGCCCGAAGA GCTCTTCTTC
    TGCCGCAAGC TGCGGACGTT GCTTCTGGGC GACAACCAGC TGAGCCAGCT CTCGCCCCAC
    GTGGGTGCCC TCAGAGCCCT CAGCCGCCTG GAGCTCAAAG GCAACCGCTT AGAGGCGCTG
    CCAGAAGAAC TTGGCAACTG TGGGGGGCTC AAGAAGGCGG GGCTCCTGGT GGAAGACACG
    CTTTACCAGG GTCTGCCGGC AGAAGTGCGG GACAAGATGG AGGAGGAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu00041
    Clone ID Related Accession (Same CDS sequence) NM_001268284.2 , NM_025061.6 , NM_025061.5 , XM_011528319.2 , XM_011528319.1
    Accession Version XM_011528319.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2391bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product volume-regulated anion channel subunit LRRC8E isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATCCCAG TGGCCGAGTT CAAGCAGTTC ACGGAACAGC AGCCTGCGTT CAAGGTGCTC 
    AAACCCTGGT GGGACGTGCT GGCCGAGTAC CTCACCGTGG CCATGCTCAT GATTGGGGTC
    TTTGGCTGCA CCCTCCAGGT GACACAGGAC AAGATCATCT GTCTACCCAA TCATGAGCTC
    CAGGAGAACT TATCAGAGGC CCCGTGCCAG CAATTGCTGC CTCGGGGGAT CCCTGAGCAG
    ATTGGGGCCC TGCAGGAGGT TAAAGGCCTT AAGAACAATT TGGACCTGCA GCAATACAGC
    TTTATTAACC AGCTGTGTTA TGAGACGGCC CTGCACTGGT ATGCCAAGTA CTTCCCTTAC
    CTCGTGGTCA TTCACACACT CATCTTCATG GTCTGCACCA GTTTCTGGTT CAAGTTCCCT
    GGCACCAGCT CCAAGATTGA ACACTTCATC TCCATCCTGG GCAAGTGTTT CGACTCTCCA
    TGGACCACCA GGGCCCTATC CGAGGTCTCC GGGGAGAACC AGAAGGGCCC AGCAGCCACC
    GAACGGGCTG CGGCCACCAT AGTGGCCATG GCAGGGACCG GGCCGGGGAA GGCAGGGGAG
    GGTGAGAAGG AGAAAGTGCT GGCGGAACCG GAGAAGGTGG TGACCGAGCC TCCAGTTGTC
    ACCCTGTTGG ACAAGAAGGA GGGTGAGCAA GCCAAAGCCC TGTTTGAGAA GGTGAAGAAG
    TTCCGCATGC ACGTGGAAGA GGGCGACATC CTGTACACCA TGTACATCCG ACAGACGGTG
    CTGAAAGTGT GTAAGTTCCT GGCCATCCTG GTCTACAACC TGGTCTATGT GGAGAAGATC
    AGTTTCCTGG TGGCCTGTAG GGTGGAGACG TCAGAGGTCA CGGGCTACGC CAGCTTCTGC
    TGCAACCACA CCAAGGCCCA CCTCTTCTCC AAGCTGGCCT TCTGTTACAT CTCCTTTGTG
    TGCATCTACG GACTTACCTG CATCTACACG CTCTACTGGC TCTTCCACCG GCCCCTCAAG
    GAGTACTCCT TCCGTTCCGT GCGGGAGGAG ACTGGCATGG GGGACATTCC TGACGTCAAG
    AATGACTTCG CCTTCATGCT GCACCTCATC GATCAGTACG ACTCCCTCTA CTCCAAGCGC
    TTCGCCGTCT TCCTGTCCGA GGTCAGCGAA AGCCGTCTAA AGCAGCTCAA TCTCAACCAC
    GAGTGGACGC CCGAGAAGCT TCGACAGAAG CTGCAGCGCA ATGCCGCGGG CCGGCTGGAG
    CTGGCCCTCT GCATGCTGCC GGGTCTGCCC GACACCGTCT TTGAGCTCAG TGAGGTGGAG
    TCACTCAGGC TGGAGGCCAT CTGCGATATC ACCTTCCCCC CGGGGCTGTC ACAGCTGGTG
    CACTTGCAGG AGCTCAGCTT GCTCCACTCG CCCGCCAGGC TACCCTTCTC CTTGCAGGTC
    TTCCTGCGGG ACCACCTGAA GGTGATGCGC GTCAAATGCG AGGAGCTCCG CGAGGTGCCG
    CTTTGGGTGT TTGGGCTGCG GGGCTTGGAG GAGCTGCACC TGGAGGGGCT TTTCCCCCAG
    GAGCTAGCTC GGGCAGCCAC CCTGGAGAGC CTCCGGGAGC TGAAGCAGCT CAAGGTGTTG
    TCCCTCCGGA GCAACGCCGG GAAGGTGCCA GCCAGTGTGA CCGACGTTGC TGGCCACCTG
    CAGAGGCTCA GCCTGCACAA CGATGGGGCC CGTCTGGTTG CCCTGAACAG CCTCAAGAAG
    CTGGCGGCAT TGCGGGAGCT GGAGCTGGTG GCCTGCGGGC TGGAGCGCAT CCCCCATGCA
    GTGTTCAGCC TGGGTGCGCT GCAGGAACTT GACCTCAAGG ACAACCACCT GCGCTCCATC
    GAGGAAATCC TCAGCTTCCA GCACTGCCGG AAGCTGGTCA CGCTCAGGCT GTGGCACAAC
    CAGATCGCCT ACGTCCCTGA GCACGTGCGG AAGCTCAGGA GCCTGGAGCA GCTCTACCTC
    AGCTACAACA AGCTGGAGAC CCTGCCCTCC CAGCTCGGCC TGTGCTCAGG CCTCCGTCTG
    CTGGATGTGT CCCACAATGG GCTACACTCC CTGCCACCCG AGGTGGGCCT CCTGCAGAAC
    CTACAGCACC TGGCCCTCTC CTACAATGCC CTGGAGGCCC TGCCCGAAGA GCTCTTCTTC
    TGCCGCAAGC TGCGGACGTT GCTTCTGGGC GACAACCAGC TGAGCCAGCT CTCGCCCCAC
    GTGGGTGCCC TCAGAGCCCT CAGCCGCCTG GAGCTCAAAG GCAACCGCTT AGAGGCGCTG
    CCAGAAGAAC TTGGCAACTG TGGGGGGCTC AAGAAGGCGG GGCTCCTGGT GGAAGACACG
    CTTTACCAGG GTCTGCCGGC AGAAGTGCGG GACAAGATGG AGGAGGAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011526621.1
    Translation

    Target ORF information:

    RefSeq Version XM_011528319.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens leucine rich repeat containing 8 family, member E (LRRC8E), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011528319.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATCCCAG TGGCCGAGTT CAAGCAGTTC ACGGAACAGC AGCCTGCGTT CAAGGTGCTC 
    AAACCCTGGT GGGACGTGCT GGCCGAGTAC CTCACCGTGG CCATGCTCAT GATTGGGGTC
    TTTGGCTGCA CCCTCCAGGT GACACAGGAC AAGATCATCT GTCTACCCAA TCATGAGCTC
    CAGGAGAACT TATCAGAGGC CCCGTGCCAG CAATTGCTGC CTCGGGGGAT CCCTGAGCAG
    ATTGGGGCCC TGCAGGAGGT TAAAGGCCTT AAGAACAATT TGGACCTGCA GCAATACAGC
    TTTATTAACC AGCTGTGTTA TGAGACGGCC CTGCACTGGT ATGCCAAGTA CTTCCCTTAC
    CTCGTGGTCA TTCACACACT CATCTTCATG GTCTGCACCA GTTTCTGGTT CAAGTTCCCT
    GGCACCAGCT CCAAGATTGA ACACTTCATC TCCATCCTGG GCAAGTGTTT CGACTCTCCA
    TGGACCACCA GGGCCCTATC CGAGGTCTCC GGGGAGAACC AGAAGGGCCC AGCAGCCACC
    GAACGGGCTG CGGCCACCAT AGTGGCCATG GCAGGGACCG GGCCGGGGAA GGCAGGGGAG
    GGTGAGAAGG AGAAAGTGCT GGCGGAACCG GAGAAGGTGG TGACCGAGCC TCCAGTTGTC
    ACCCTGTTGG ACAAGAAGGA GGGTGAGCAA GCCAAAGCCC TGTTTGAGAA GGTGAAGAAG
    TTCCGCATGC ACGTGGAAGA GGGCGACATC CTGTACACCA TGTACATCCG ACAGACGGTG
    CTGAAAGTGT GTAAGTTCCT GGCCATCCTG GTCTACAACC TGGTCTATGT GGAGAAGATC
    AGTTTCCTGG TGGCCTGTAG GGTGGAGACG TCAGAGGTCA CGGGCTACGC CAGCTTCTGC
    TGCAACCACA CCAAGGCCCA CCTCTTCTCC AAGCTGGCCT TCTGTTACAT CTCCTTTGTG
    TGCATCTACG GACTTACCTG CATCTACACG CTCTACTGGC TCTTCCACCG GCCCCTCAAG
    GAGTACTCCT TCCGTTCCGT GCGGGAGGAG ACTGGCATGG GGGACATTCC TGACGTCAAG
    AATGACTTCG CCTTCATGCT GCACCTCATC GATCAGTACG ACTCCCTCTA CTCCAAGCGC
    TTCGCCGTCT TCCTGTCCGA GGTCAGCGAA AGCCGTCTAA AGCAGCTCAA TCTCAACCAC
    GAGTGGACGC CCGAGAAGCT TCGACAGAAG CTGCAGCGCA ATGCCGCGGG CCGGCTGGAG
    CTGGCCCTCT GCATGCTGCC GGGTCTGCCC GACACCGTCT TTGAGCTCAG TGAGGTGGAG
    TCACTCAGGC TGGAGGCCAT CTGCGATATC ACCTTCCCCC CGGGGCTGTC ACAGCTGGTG
    CACTTGCAGG AGCTCAGCTT GCTCCACTCG CCCGCCAGGC TACCCTTCTC CTTGCAGGTC
    TTCCTGCGGG ACCACCTGAA GGTGATGCGC GTCAAATGCG AGGAGCTCCG CGAGGTGCCG
    CTTTGGGTGT TTGGGCTGCG GGGCTTGGAG GAGCTGCACC TGGAGGGGCT TTTCCCCCAG
    GAGCTAGCTC GGGCAGCCAC CCTGGAGAGC CTCCGGGAGC TGAAGCAGCT CAAGGTGTTG
    TCCCTCCGGA GCAACGCCGG GAAGGTGCCA GCCAGTGTGA CCGACGTTGC TGGCCACCTG
    CAGAGGCTCA GCCTGCACAA CGATGGGGCC CGTCTGGTTG CCCTGAACAG CCTCAAGAAG
    CTGGCGGCAT TGCGGGAGCT GGAGCTGGTG GCCTGCGGGC TGGAGCGCAT CCCCCATGCA
    GTGTTCAGCC TGGGTGCGCT GCAGGAACTT GACCTCAAGG ACAACCACCT GCGCTCCATC
    GAGGAAATCC TCAGCTTCCA GCACTGCCGG AAGCTGGTCA CGCTCAGGCT GTGGCACAAC
    CAGATCGCCT ACGTCCCTGA GCACGTGCGG AAGCTCAGGA GCCTGGAGCA GCTCTACCTC
    AGCTACAACA AGCTGGAGAC CCTGCCCTCC CAGCTCGGCC TGTGCTCAGG CCTCCGTCTG
    CTGGATGTGT CCCACAATGG GCTACACTCC CTGCCACCCG AGGTGGGCCT CCTGCAGAAC
    CTACAGCACC TGGCCCTCTC CTACAATGCC CTGGAGGCCC TGCCCGAAGA GCTCTTCTTC
    TGCCGCAAGC TGCGGACGTT GCTTCTGGGC GACAACCAGC TGAGCCAGCT CTCGCCCCAC
    GTGGGTGCCC TCAGAGCCCT CAGCCGCCTG GAGCTCAAAG GCAACCGCTT AGAGGCGCTG
    CCAGAAGAAC TTGGCAACTG TGGGGGGCTC AAGAAGGCGG GGCTCCTGGT GGAAGACACG
    CTTTACCAGG GTCTGCCGGC AGAAGTGCGG GACAAGATGG AGGAGGAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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