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NCK2 cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol NCK2
Entrez Gene ID 8440
Full Name NCK adaptor protein 2
Synonyms GRB4,NCKbeta
General protein information
Preferred Names

NCK adaptor protein 2

Names

cytoplasmic protein NCK2
SH2/SH3 adaptor protein NCK-beta
growth factor receptor-bound protein 4
noncatalytic region of tyrosine kinase, beta
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

2

2q12.2
Summary This gene encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008].
Disorder MIM:

604930


Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_011511992.2 XP_011510294.2 cytoplasmic protein NCK2 isoform X2
NM_001004722.3 NP_001004722.1 cytoplasmic protein NCK2 isoform B
XM_017005104.1 XP_016860593.1 cytoplasmic protein NCK2 isoform X1
XM_017005105.1 XP_016860594.1 cytoplasmic protein NCK2 isoform X1
NM_001004720.2 NP_001004720.1 cytoplasmic protein NCK2 isoform A
XM_011511991.1 XP_011510293.1 cytoplasmic protein NCK2 isoform X1
XM_017005103.1 XP_016860592.1 cytoplasmic protein NCK2 isoform X1
XM_006712797.3 XP_006712860.1 cytoplasmic protein NCK2 isoform X1
NM_003581.4 NP_003572.2 cytoplasmic protein NCK2 isoform A
XM_006712797.2 XP_006712860.1 cytoplasmic protein NCK2 isoform X1
XM_011511991.2 XP_011510293.1 cytoplasmic protein NCK2 isoform X1

Pathways from BioSystems


Homologies


Xenopus tropicalis (tropical clawed frog) nck2 NP_001007985.1
Pan troglodytes (chimpanzee) NCK2 XP_003309223.1
Canis lupus familiaris (dog) NCK2 XP_005626042.1
Mus musculus (house mouse) Nck2 NP_035009.3
Danio rerio (zebrafish) nck2b NP_001003492.2
Gallus gallus (chicken) NCK2 NP_001025985.1
Homo sapiens (human) NCK2 NP_003572.2
Bos taurus (cattle) NCK2 NP_001092506.1
Caenorhabditis elegans (roundworm) nck-1 NP_508706.2
NCK2 XP_001109452.2
Rattus norvegicus (Norway rat) Nck2 NP_001101686.1

Gene Ontology


Bibliography

Related articles in PubMed

Nephrin Suppresses Hippo Signaling through the Adaptor Proteins Nck and WTIP.
Keyvani Chahi A, Martin CE, Jones N
The Journal of biological chemistry291(24)12799-808(2016 Jun)

An inter-species protein-protein interaction network across vast evolutionary distance.
Zhong Q, Pevzner SJ, Hao T, Wang Y, Mosca R, Menche J, Taipale M, Ta?an M, Fan C, Yang X, Haley P, Murray RR, Mer F, Gebreab F, Tam S, MacWilliams A, Dricot A, Reichert P, Santhanam B, Ghamsari L, Calderwood MA, Rolland T, Charloteaux B, Lindquist S, Barab?si AL, Hill DE, Aloy P, Cusick ME, Xia Y, Roth FP, Vidal M
Molecular systems biology12(4)865(2016 Apr)

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Yang X, Coulombe-Huntington J, Kang S, Sheynkman GM, Hao T, Richardson A, Sun S, Yang F, Shen YA, Murray RR, Spirohn K, Begg BE, Duran-Frigola M, MacWilliams A, Pevzner SJ, Zhong Q, Trigg SA, Tam S, Ghamsari L, Sahni N, Yi S, Rodriguez MD, Balcha D, Tan G, Costanzo M, Andrews B, Boone C, Zhou XJ, Salehi-Ashtiani K, Charloteaux B, Chen AA, Calderwood MA, Aloy P, Roth FP, Hill DE, Iakoucheva LM, Xia Y, Vidal M
Cell164(4)805-17(2016 Feb)

Targeting of the EGFR/?1 integrin connecting proteins PINCH1 and Nck2 radiosensitizes three-dimensional SCC cell cultures.
Rossow L, Eke I, Dickreuter E, Cordes N
Oncology reports34(1)469-76(2015 Jul)

Solution structure of the first SRC homology 3 domain of human Nck2.
Park S, Takeuchi K, Wagner G
Journal of biomolecular NMR34(3)203-8(2006 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Nck1 and Nck2 Interact with WTIP. Nck1/2 integrates nephrin with the Hippo kinase cascade through association with the adaptor protein WTIP.
Title: Nephrin Suppresses Hippo Signaling through the Adaptor Proteins Nck and WTIP.

Data suggest that PINCH1 and Nck2 critically participate in the regulation of cellular radiosensitivity and EGFR function and downstream signaling in a cellular model of human squamous cell carcinoma.
Title: Targeting of the EGFR/?1 integrin connecting proteins PINCH1 and Nck2 radiosensitizes three-dimensional SCC cell cultures.

Tir-Intimin interaction recruits the Nck adaptor to a Tir tyrosine phosphorylated residue where it activates neural Wiskott-Aldrich syndrome protein (N-WASP).
Title: Nck adaptors, besides promoting N-WASP mediated actin-nucleation activity at pedestals, influence the cellular levels of enteropathogenic Escherichia coli Tir effector.

Proteasomal degradation of Nck1 but not Nck2 regulates RhoA activation and actin dynamics.
Title: Proteasomal degradation of Nck1 but not Nck2 regulates RhoA activation and actin dynamics.

NCK2 is involved in the susceptibility to opiates addiction.
Title: NCK2 is significantly associated with opiates addiction in African-origin men.

The following NCK2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCK2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu14522 NM_001004720.2
Latest version!		 Homo sapiens NCK adaptor protein 2 (NCK2), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu14522 NM_003581.4
Latest version!		 Homo sapiens NCK adaptor protein 2 (NCK2), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu14522 XM_017005105.1
Latest version!		 Homo sapiens NCK adaptor protein 2 (NCK2), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu14522 XM_006712797.3
Latest version!		 Homo sapiens NCK adaptor protein 2 (NCK2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu14522 XM_017005103.1
Latest version!		 Homo sapiens NCK adaptor protein 2 (NCK2), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu14522 XM_017005104.1
Latest version!		 Homo sapiens NCK adaptor protein 2 (NCK2), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu14522 XM_011511991.2
Latest version!		 Homo sapiens NCK adaptor protein 2 (NCK2), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu14299 NM_001004722.3
Latest version!		 Homo sapiens NCK adaptor protein 2 (NCK2), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu14299 XM_011511992.2
Latest version!		 Homo sapiens NCK adaptor protein 2 (NCK2), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
View Old Accession Versions >>
OHu14522 XM_006712797.2 Homo sapiens NCK adaptor protein 2 (NCK2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu14522 XM_011511991.1 Homo sapiens NCK adaptor protein 2 (NCK2), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu14522
Clone ID Related Accession (Same CDS sequence) XM_017005104.1 , XM_017005105.1 , NM_001004720.2 , XM_011511991.1 , XM_017005103.1 , XM_006712797.3 , NM_003581.4 , XM_006712797.2 , XM_011511991.2
Accession Version NM_001004720.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1143bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-10-02
Organism Homo sapiens(human)
Product cytoplasmic protein NCK2 isoform A
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB456958.1, BC000103.2, AC009505.3 and BU738133.1. On Jun 5, 2010 this sequence version replaced NM_001004720.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform (A). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC000103.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGACAGAAG AAGTTATTGT GATAGCCAAG TGGGACTACA CCGCCCAGCA GGACCAGGAG 
CTGGACATCA AGAAGAACGA GCGGCTGTGG TTGCTGGACG ACTCCAAGAC GTGGTGGCGG 
GTGAGGAACG CGGCCAACAG GACGGGCTAT GTACCGTCCA ACTACGTGGA GCGGAAGAAC 
AGCCTGAAGA AGGGCTCCCT CGTGAAGAAC CTGAAGGACA CACTAGGCCT CGGCAAGACG 
CGCAGGAAGA CCAGCGCGCG GGATGCGTCC CCCACGCCCA GCACGGACGC CGAGTACCCC 
GCCAATGGCA GCGGCGCCGA CCGCATCTAC GACCTCAACA TCCCGGCCTT CGTCAAGTTC 
GCCTATGTGG CCGAGCGGGA GGATGAGTTG TCCCTGGTGA AGGGGTCGCG CGTCACCGTC 
ATGGAGAAGT GCAGCGACGG TTGGTGGCGG GGCAGCTACA ACGGGCAGAT CGGCTGGTTC 
CCCTCCAACT ACGTCTTGGA GGAGGTGGAC GAGGCGGCTG CGGAGTCCCC AAGCTTCCTG 
AGCCTGCGCA AGGGCGCCTC GCTGAGCAAT GGCCAGGGCT CCCGCGTGCT GCATGTGGTC 
CAGACGCTGT ACCCCTTCAG CTCAGTCACC GAGGAGGAGC TCAACTTCGA GAAGGGGGAG 
ACCATGGAGG TGATTGAGAA GCCGGAGAAC GACCCCGAGT GGTGGAAATG CAAAAATGCC 
CGGGGCCAGG TGGGCCTCGT CCCCAAAAAC TACGTGGTGG TCCTCAGTGA CGGGCCTGCC 
CTGCACCCTG CGCACGCCCC ACAGATAAGC TACACCGGGC CCTCGTCCAG CGGGCGCTTC 
GCGGGCAGAG AGTGGTACTA CGGGAACGTG ACGCGGCACC AGGCCGAGTG CGCCCTCAAC 
GAGCGGGGCG TGGAGGGCGA CTTCCTCATT AGGGACAGCG AGTCCTCGCC CAGCGACTTC 
TCCGTGTCCC TTAAAGCGTC AGGGAAGAAC AAACACTTCA AGGTGCAGCT CGTGGACAAT 
GTCTACTGCA TTGGGCAGCG GCGCTTCCAC ACCATGGACG AGCTGGTGGA ACACTACAAA 
AAGGCGCCCA TCTTCACCAG CGAGCACGGG GAGAAGCTCT ACCTCGTCAG GGCCCTGCAG 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001004720.1
CDS167..1309
Misc Feature(1)161..163
Misc Feature(2)170..343
Misc Feature(3)170..172
Misc Feature(4)order(203..205,212..217,221..226,272..280,314..316,320..331)
Misc Feature(5)434..436
Misc Feature(6)440..442
Misc Feature(7)446..448
Misc Feature(8)494..496
Misc Feature(9)506..670
Misc Feature(10)order(524..526,530..532,539..541,551..553,605..610,647..649,653..658)
Misc Feature(11)758..934
Misc Feature(12)order(776..778,782..784,791..793,803..805,863..868,908..910,914..919)
Misc Feature(13)order(869..871,911..916,920..928)
Misc Feature(14)1013..1306
Misc Feature(15)order(1040..1042,1097..1099,1103..1111,1127..1129,1166..1168)
Misc Feature(16)order(1163..1165,1196..1198,1205..1207,1256..1264)
Exon (1)1..150
Exon (2)151..392
Exon (3)393..1114
Exon (4)1115..2407
Translation

Target ORF information:

RefSeq Version NM_001004720.2
Organism Homo sapiens(human)
Definition Homo sapiens NCK adaptor protein 2 (NCK2), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001004720.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGACAGAAG AAGTTATTGT GATAGCCAAG TGGGACTACA CCGCCCAGCA GGACCAGGAG 
CTGGACATCA AGAAGAACGA GCGGCTGTGG TTGCTGGACG ACTCCAAGAC GTGGTGGCGG 
GTGAGGAACG CGGCCAACAG GACGGGCTAT GTACCGTCCA ACTACGTGGA GCGGAAGAAC 
AGCCTGAAGA AGGGCTCCCT CGTGAAGAAC CTGAAGGACA CACTAGGCCT CGGCAAGACG 
CGCAGGAAGA CCAGCGCGCG GGATGCGTCC CCCACGCCCA GCACGGACGC CGAGTACCCC 
GCCAATGGCA GCGGCGCCGA CCGCATCTAC GACCTCAACA TCCCGGCCTT CGTCAAGTTC 
GCCTATGTGG CCGAGCGGGA GGATGAGTTG TCCCTGGTGA AGGGGTCGCG CGTCACCGTC 
ATGGAGAAGT GCAGCGACGG TTGGTGGCGG GGCAGCTACA ACGGGCAGAT CGGCTGGTTC 
CCCTCCAACT ACGTCTTGGA GGAGGTGGAC GAGGCGGCTG CGGAGTCCCC AAGCTTCCTG 
AGCCTGCGCA AGGGCGCCTC GCTGAGCAAT GGCCAGGGCT CCCGCGTGCT GCATGTGGTC 
CAGACGCTGT ACCCCTTCAG CTCAGTCACC GAGGAGGAGC TCAACTTCGA GAAGGGGGAG 
ACCATGGAGG TGATTGAGAA GCCGGAGAAC GACCCCGAGT GGTGGAAATG CAAAAATGCC 
CGGGGCCAGG TGGGCCTCGT CCCCAAAAAC TACGTGGTGG TCCTCAGTGA CGGGCCTGCC 
CTGCACCCTG CGCACGCCCC ACAGATAAGC TACACCGGGC CCTCGTCCAG CGGGCGCTTC 
GCGGGCAGAG AGTGGTACTA CGGGAACGTG ACGCGGCACC AGGCCGAGTG CGCCCTCAAC 
GAGCGGGGCG TGGAGGGCGA CTTCCTCATT AGGGACAGCG AGTCCTCGCC CAGCGACTTC 
TCCGTGTCCC TTAAAGCGTC AGGGAAGAAC AAACACTTCA AGGTGCAGCT CGTGGACAAT 
GTCTACTGCA TTGGGCAGCG GCGCTTCCAC ACCATGGACG AGCTGGTGGA ACACTACAAA 
AAGGCGCCCA TCTTCACCAG CGAGCACGGG GAGAAGCTCT ACCTCGTCAG GGCCCTGCAG 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu14522
Clone ID Related Accession (Same CDS sequence) XM_017005104.1 , XM_017005105.1 , NM_001004720.2 , XM_011511991.1 , XM_017005103.1 , XM_006712797.3 , NM_003581.4 , XM_006712797.2 , XM_011511991.2
Accession Version NM_003581.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1143bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-10-03
Organism Homo sapiens(human)
Product cytoplasmic protein NCK2 isoform A
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010978.8, BC007195.1 and AC009505.3. On Jul 30, 2011 this sequence version replaced NM_003581.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (A). Variants 1 and 2 both encode isoform A. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: SRR1163655.171588.1, SRR1660807.254986.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGACAGAAG AAGTTATTGT GATAGCCAAG TGGGACTACA CCGCCCAGCA GGACCAGGAG 
CTGGACATCA AGAAGAACGA GCGGCTGTGG TTGCTGGACG ACTCCAAGAC GTGGTGGCGG 
GTGAGGAACG CGGCCAACAG GACGGGCTAT GTACCGTCCA ACTACGTGGA GCGGAAGAAC 
AGCCTGAAGA AGGGCTCCCT CGTGAAGAAC CTGAAGGACA CACTAGGCCT CGGCAAGACG 
CGCAGGAAGA CCAGCGCGCG GGATGCGTCC CCCACGCCCA GCACGGACGC CGAGTACCCC 
GCCAATGGCA GCGGCGCCGA CCGCATCTAC GACCTCAACA TCCCGGCCTT CGTCAAGTTC 
GCCTATGTGG CCGAGCGGGA GGATGAGTTG TCCCTGGTGA AGGGGTCGCG CGTCACCGTC 
ATGGAGAAGT GCAGCGACGG TTGGTGGCGG GGCAGCTACA ACGGGCAGAT CGGCTGGTTC 
CCCTCCAACT ACGTCTTGGA GGAGGTGGAC GAGGCGGCTG CGGAGTCCCC AAGCTTCCTG 
AGCCTGCGCA AGGGCGCCTC GCTGAGCAAT GGCCAGGGCT CCCGCGTGCT GCATGTGGTC 
CAGACGCTGT ACCCCTTCAG CTCAGTCACC GAGGAGGAGC TCAACTTCGA GAAGGGGGAG 
ACCATGGAGG TGATTGAGAA GCCGGAGAAC GACCCCGAGT GGTGGAAATG CAAAAATGCC 
CGGGGCCAGG TGGGCCTCGT CCCCAAAAAC TACGTGGTGG TCCTCAGTGA CGGGCCTGCC 
CTGCACCCTG CGCACGCCCC ACAGATAAGC TACACCGGGC CCTCGTCCAG CGGGCGCTTC 
GCGGGCAGAG AGTGGTACTA CGGGAACGTG ACGCGGCACC AGGCCGAGTG CGCCCTCAAC 
GAGCGGGGCG TGGAGGGCGA CTTCCTCATT AGGGACAGCG AGTCCTCGCC CAGCGACTTC 
TCCGTGTCCC TTAAAGCGTC AGGGAAGAAC AAACACTTCA AGGTGCAGCT CGTGGACAAT 
GTCTACTGCA TTGGGCAGCG GCGCTTCCAC ACCATGGACG AGCTGGTGGA ACACTACAAA 
AAGGCGCCCA TCTTCACCAG CGAGCACGGG GAGAAGCTCT ACCTCGTCAG GGCCCTGCAG 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_003572.2
CDS277..1419
Misc Feature(1)271..273
Misc Feature(2)280..453
Misc Feature(3)280..282
Misc Feature(4)order(313..315,322..327,331..336,382..390,424..426,430..441)
Misc Feature(5)544..546
Misc Feature(6)550..552
Misc Feature(7)556..558
Misc Feature(8)604..606
Misc Feature(9)616..780
Misc Feature(10)order(634..636,640..642,649..651,661..663,715..720,757..759,763..768)
Misc Feature(11)868..1044
Misc Feature(12)order(886..888,892..894,901..903,913..915,973..978,1018..1020,1024..1029)
Misc Feature(13)order(979..981,1021..1026,1030..1038)
Misc Feature(14)1123..1416
Misc Feature(15)order(1150..1152,1207..1209,1213..1221,1237..1239,1276..1278)
Misc Feature(16)order(1273..1275,1306..1308,1315..1317,1366..1374)
Exon (1)1..76
Exon (2)77..260
Exon (3)261..502
Exon (4)503..1224
Exon (5)1225..2517
Translation

Target ORF information:

RefSeq Version NM_003581.4
Organism Homo sapiens(human)
Definition Homo sapiens NCK adaptor protein 2 (NCK2), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_003581.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGACAGAAG AAGTTATTGT GATAGCCAAG TGGGACTACA CCGCCCAGCA GGACCAGGAG 
CTGGACATCA AGAAGAACGA GCGGCTGTGG TTGCTGGACG ACTCCAAGAC GTGGTGGCGG 
GTGAGGAACG CGGCCAACAG GACGGGCTAT GTACCGTCCA ACTACGTGGA GCGGAAGAAC 
AGCCTGAAGA AGGGCTCCCT CGTGAAGAAC CTGAAGGACA CACTAGGCCT CGGCAAGACG 
CGCAGGAAGA CCAGCGCGCG GGATGCGTCC CCCACGCCCA GCACGGACGC CGAGTACCCC 
GCCAATGGCA GCGGCGCCGA CCGCATCTAC GACCTCAACA TCCCGGCCTT CGTCAAGTTC 
GCCTATGTGG CCGAGCGGGA GGATGAGTTG TCCCTGGTGA AGGGGTCGCG CGTCACCGTC 
ATGGAGAAGT GCAGCGACGG TTGGTGGCGG GGCAGCTACA ACGGGCAGAT CGGCTGGTTC 
CCCTCCAACT ACGTCTTGGA GGAGGTGGAC GAGGCGGCTG CGGAGTCCCC AAGCTTCCTG 
AGCCTGCGCA AGGGCGCCTC GCTGAGCAAT GGCCAGGGCT CCCGCGTGCT GCATGTGGTC 
CAGACGCTGT ACCCCTTCAG CTCAGTCACC GAGGAGGAGC TCAACTTCGA GAAGGGGGAG 
ACCATGGAGG TGATTGAGAA GCCGGAGAAC GACCCCGAGT GGTGGAAATG CAAAAATGCC 
CGGGGCCAGG TGGGCCTCGT CCCCAAAAAC TACGTGGTGG TCCTCAGTGA CGGGCCTGCC 
CTGCACCCTG CGCACGCCCC ACAGATAAGC TACACCGGGC CCTCGTCCAG CGGGCGCTTC 
GCGGGCAGAG AGTGGTACTA CGGGAACGTG ACGCGGCACC AGGCCGAGTG CGCCCTCAAC 
GAGCGGGGCG TGGAGGGCGA CTTCCTCATT AGGGACAGCG AGTCCTCGCC CAGCGACTTC 
TCCGTGTCCC TTAAAGCGTC AGGGAAGAAC AAACACTTCA AGGTGCAGCT CGTGGACAAT 
GTCTACTGCA TTGGGCAGCG GCGCTTCCAC ACCATGGACG AGCTGGTGGA ACACTACAAA 
AAGGCGCCCA TCTTCACCAG CGAGCACGGG GAGAAGCTCT ACCTCGTCAG GGCCCTGCAG 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu14522
Clone ID Related Accession (Same CDS sequence) XM_017005104.1 , XM_017005105.1 , NM_001004720.2 , XM_011511991.1 , XM_017005103.1 , XM_006712797.3 , NM_003581.4 , XM_006712797.2 , XM_011511991.2
Accession Version XM_017005104.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1143bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product cytoplasmic protein NCK2 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGACAGAAG AAGTTATTGT GATAGCCAAG TGGGACTACA CCGCCCAGCA GGACCAGGAG 
CTGGACATCA AGAAGAACGA GCGGCTGTGG TTGCTGGACG ACTCCAAGAC GTGGTGGCGG 
GTGAGGAACG CGGCCAACAG GACGGGCTAT GTACCGTCCA ACTACGTGGA GCGGAAGAAC 
AGCCTGAAGA AGGGCTCCCT CGTGAAGAAC CTGAAGGACA CACTAGGCCT CGGCAAGACG 
CGCAGGAAGA CCAGCGCGCG GGATGCGTCC CCCACGCCCA GCACGGACGC CGAGTACCCC 
GCCAATGGCA GCGGCGCCGA CCGCATCTAC GACCTCAACA TCCCGGCCTT CGTCAAGTTC 
GCCTATGTGG CCGAGCGGGA GGATGAGTTG TCCCTGGTGA AGGGGTCGCG CGTCACCGTC 
ATGGAGAAGT GCAGCGACGG TTGGTGGCGG GGCAGCTACA ACGGGCAGAT CGGCTGGTTC 
CCCTCCAACT ACGTCTTGGA GGAGGTGGAC GAGGCGGCTG CGGAGTCCCC AAGCTTCCTG 
AGCCTGCGCA AGGGCGCCTC GCTGAGCAAT GGCCAGGGCT CCCGCGTGCT GCATGTGGTC 
CAGACGCTGT ACCCCTTCAG CTCAGTCACC GAGGAGGAGC TCAACTTCGA GAAGGGGGAG 
ACCATGGAGG TGATTGAGAA GCCGGAGAAC GACCCCGAGT GGTGGAAATG CAAAAATGCC 
CGGGGCCAGG TGGGCCTCGT CCCCAAAAAC TACGTGGTGG TCCTCAGTGA CGGGCCTGCC 
CTGCACCCTG CGCACGCCCC ACAGATAAGC TACACCGGGC CCTCGTCCAG CGGGCGCTTC 
GCGGGCAGAG AGTGGTACTA CGGGAACGTG ACGCGGCACC AGGCCGAGTG CGCCCTCAAC 
GAGCGGGGCG TGGAGGGCGA CTTCCTCATT AGGGACAGCG AGTCCTCGCC CAGCGACTTC 
TCCGTGTCCC TTAAAGCGTC AGGGAAGAAC AAACACTTCA AGGTGCAGCT CGTGGACAAT 
GTCTACTGCA TTGGGCAGCG GCGCTTCCAC ACCATGGACG AGCTGGTGGA ACACTACAAA 
AAGGCGCCCA TCTTCACCAG CGAGCACGGG GAGAAGCTCT ACCTCGTCAG GGCCCTGCAG 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016860593.1
CDS547..1689
Misc Feature(1)550..723
Misc Feature(2)order(583..585,592..597,601..606,652..660,694..696,700..711)
Misc Feature(3)886..1050
Misc Feature(4)order(904..906,910..912,919..921,931..933,985..990,1027..1029,1033..1038)
Misc Feature(5)1138..1314
Misc Feature(6)order(1156..1158,1162..1164,1171..1173,1183..1185,1243..1248,1288..1290,1294..1299)
Misc Feature(7)order(1249..1251,1291..1296,1300..1308)
Misc Feature(8)1393..1686
Misc Feature(9)order(1420..1422,1477..1479,1483..1491,1507..1509,1546..1548)
Misc Feature(10)order(1543..1545,1576..1578,1585..1587,1636..1644)
Translation

Target ORF information:

RefSeq Version XM_017005104.1
Organism Homo sapiens(human)
Definition Homo sapiens NCK adaptor protein 2 (NCK2), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017005104.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGACAGAAG AAGTTATTGT GATAGCCAAG TGGGACTACA CCGCCCAGCA GGACCAGGAG 
CTGGACATCA AGAAGAACGA GCGGCTGTGG TTGCTGGACG ACTCCAAGAC GTGGTGGCGG 
GTGAGGAACG CGGCCAACAG GACGGGCTAT GTACCGTCCA ACTACGTGGA GCGGAAGAAC 
AGCCTGAAGA AGGGCTCCCT CGTGAAGAAC CTGAAGGACA CACTAGGCCT CGGCAAGACG 
CGCAGGAAGA CCAGCGCGCG GGATGCGTCC CCCACGCCCA GCACGGACGC CGAGTACCCC 
GCCAATGGCA GCGGCGCCGA CCGCATCTAC GACCTCAACA TCCCGGCCTT CGTCAAGTTC 
GCCTATGTGG CCGAGCGGGA GGATGAGTTG TCCCTGGTGA AGGGGTCGCG CGTCACCGTC 
ATGGAGAAGT GCAGCGACGG TTGGTGGCGG GGCAGCTACA ACGGGCAGAT CGGCTGGTTC 
CCCTCCAACT ACGTCTTGGA GGAGGTGGAC GAGGCGGCTG CGGAGTCCCC AAGCTTCCTG 
AGCCTGCGCA AGGGCGCCTC GCTGAGCAAT GGCCAGGGCT CCCGCGTGCT GCATGTGGTC 
CAGACGCTGT ACCCCTTCAG CTCAGTCACC GAGGAGGAGC TCAACTTCGA GAAGGGGGAG 
ACCATGGAGG TGATTGAGAA GCCGGAGAAC GACCCCGAGT GGTGGAAATG CAAAAATGCC 
CGGGGCCAGG TGGGCCTCGT CCCCAAAAAC TACGTGGTGG TCCTCAGTGA CGGGCCTGCC 
CTGCACCCTG CGCACGCCCC ACAGATAAGC TACACCGGGC CCTCGTCCAG CGGGCGCTTC 
GCGGGCAGAG AGTGGTACTA CGGGAACGTG ACGCGGCACC AGGCCGAGTG CGCCCTCAAC 
GAGCGGGGCG TGGAGGGCGA CTTCCTCATT AGGGACAGCG AGTCCTCGCC CAGCGACTTC 
TCCGTGTCCC TTAAAGCGTC AGGGAAGAAC AAACACTTCA AGGTGCAGCT CGTGGACAAT 
GTCTACTGCA TTGGGCAGCG GCGCTTCCAC ACCATGGACG AGCTGGTGGA ACACTACAAA 
AAGGCGCCCA TCTTCACCAG CGAGCACGGG GAGAAGCTCT ACCTCGTCAG GGCCCTGCAG 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu14522
Clone ID Related Accession (Same CDS sequence) XM_017005104.1 , XM_017005105.1 , NM_001004720.2 , XM_011511991.1 , XM_017005103.1 , XM_006712797.3 , NM_003581.4 , XM_006712797.2 , XM_011511991.2
Accession Version XM_017005105.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1143bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product cytoplasmic protein NCK2 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGACAGAAG AAGTTATTGT GATAGCCAAG TGGGACTACA CCGCCCAGCA GGACCAGGAG 
CTGGACATCA AGAAGAACGA GCGGCTGTGG TTGCTGGACG ACTCCAAGAC GTGGTGGCGG 
GTGAGGAACG CGGCCAACAG GACGGGCTAT GTACCGTCCA ACTACGTGGA GCGGAAGAAC 
AGCCTGAAGA AGGGCTCCCT CGTGAAGAAC CTGAAGGACA CACTAGGCCT CGGCAAGACG 
CGCAGGAAGA CCAGCGCGCG GGATGCGTCC CCCACGCCCA GCACGGACGC CGAGTACCCC 
GCCAATGGCA GCGGCGCCGA CCGCATCTAC GACCTCAACA TCCCGGCCTT CGTCAAGTTC 
GCCTATGTGG CCGAGCGGGA GGATGAGTTG TCCCTGGTGA AGGGGTCGCG CGTCACCGTC 
ATGGAGAAGT GCAGCGACGG TTGGTGGCGG GGCAGCTACA ACGGGCAGAT CGGCTGGTTC 
CCCTCCAACT ACGTCTTGGA GGAGGTGGAC GAGGCGGCTG CGGAGTCCCC AAGCTTCCTG 
AGCCTGCGCA AGGGCGCCTC GCTGAGCAAT GGCCAGGGCT CCCGCGTGCT GCATGTGGTC 
CAGACGCTGT ACCCCTTCAG CTCAGTCACC GAGGAGGAGC TCAACTTCGA GAAGGGGGAG 
ACCATGGAGG TGATTGAGAA GCCGGAGAAC GACCCCGAGT GGTGGAAATG CAAAAATGCC 
CGGGGCCAGG TGGGCCTCGT CCCCAAAAAC TACGTGGTGG TCCTCAGTGA CGGGCCTGCC 
CTGCACCCTG CGCACGCCCC ACAGATAAGC TACACCGGGC CCTCGTCCAG CGGGCGCTTC 
GCGGGCAGAG AGTGGTACTA CGGGAACGTG ACGCGGCACC AGGCCGAGTG CGCCCTCAAC 
GAGCGGGGCG TGGAGGGCGA CTTCCTCATT AGGGACAGCG AGTCCTCGCC CAGCGACTTC 
TCCGTGTCCC TTAAAGCGTC AGGGAAGAAC AAACACTTCA AGGTGCAGCT CGTGGACAAT 
GTCTACTGCA TTGGGCAGCG GCGCTTCCAC ACCATGGACG AGCTGGTGGA ACACTACAAA 
AAGGCGCCCA TCTTCACCAG CGAGCACGGG GAGAAGCTCT ACCTCGTCAG GGCCCTGCAG 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016860594.1
CDS284..1426
Misc Feature(1)287..460
Misc Feature(2)order(320..322,329..334,338..343,389..397,431..433,437..448)
Misc Feature(3)623..787
Misc Feature(4)order(641..643,647..649,656..658,668..670,722..727,764..766,770..775)
Misc Feature(5)875..1051
Misc Feature(6)order(893..895,899..901,908..910,920..922,980..985,1025..1027,1031..1036)
Misc Feature(7)order(986..988,1028..1033,1037..1045)
Misc Feature(8)1130..1423
Misc Feature(9)order(1157..1159,1214..1216,1220..1228,1244..1246,1283..1285)
Misc Feature(10)order(1280..1282,1313..1315,1322..1324,1373..1381)
Translation

Target ORF information:

RefSeq Version XM_017005105.1
Organism Homo sapiens(human)
Definition Homo sapiens NCK adaptor protein 2 (NCK2), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017005105.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGACAGAAG AAGTTATTGT GATAGCCAAG TGGGACTACA CCGCCCAGCA GGACCAGGAG 
CTGGACATCA AGAAGAACGA GCGGCTGTGG TTGCTGGACG ACTCCAAGAC GTGGTGGCGG 
GTGAGGAACG CGGCCAACAG GACGGGCTAT GTACCGTCCA ACTACGTGGA GCGGAAGAAC 
AGCCTGAAGA AGGGCTCCCT CGTGAAGAAC CTGAAGGACA CACTAGGCCT CGGCAAGACG 
CGCAGGAAGA CCAGCGCGCG GGATGCGTCC CCCACGCCCA GCACGGACGC CGAGTACCCC 
GCCAATGGCA GCGGCGCCGA CCGCATCTAC GACCTCAACA TCCCGGCCTT CGTCAAGTTC 
GCCTATGTGG CCGAGCGGGA GGATGAGTTG TCCCTGGTGA AGGGGTCGCG CGTCACCGTC 
ATGGAGAAGT GCAGCGACGG TTGGTGGCGG GGCAGCTACA ACGGGCAGAT CGGCTGGTTC 
CCCTCCAACT ACGTCTTGGA GGAGGTGGAC GAGGCGGCTG CGGAGTCCCC AAGCTTCCTG 
AGCCTGCGCA AGGGCGCCTC GCTGAGCAAT GGCCAGGGCT CCCGCGTGCT GCATGTGGTC 
CAGACGCTGT ACCCCTTCAG CTCAGTCACC GAGGAGGAGC TCAACTTCGA GAAGGGGGAG 
ACCATGGAGG TGATTGAGAA GCCGGAGAAC GACCCCGAGT GGTGGAAATG CAAAAATGCC 
CGGGGCCAGG TGGGCCTCGT CCCCAAAAAC TACGTGGTGG TCCTCAGTGA CGGGCCTGCC 
CTGCACCCTG CGCACGCCCC ACAGATAAGC TACACCGGGC CCTCGTCCAG CGGGCGCTTC 
GCGGGCAGAG AGTGGTACTA CGGGAACGTG ACGCGGCACC AGGCCGAGTG CGCCCTCAAC 
GAGCGGGGCG TGGAGGGCGA CTTCCTCATT AGGGACAGCG AGTCCTCGCC CAGCGACTTC 
TCCGTGTCCC TTAAAGCGTC AGGGAAGAAC AAACACTTCA AGGTGCAGCT CGTGGACAAT 
GTCTACTGCA TTGGGCAGCG GCGCTTCCAC ACCATGGACG AGCTGGTGGA ACACTACAAA 
AAGGCGCCCA TCTTCACCAG CGAGCACGGG GAGAAGCTCT ACCTCGTCAG GGCCCTGCAG 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu14522
Clone ID Related Accession (Same CDS sequence) XM_017005104.1 , XM_017005105.1 , NM_001004720.2 , XM_011511991.1 , XM_017005103.1 , XM_006712797.3 , NM_003581.4 , XM_006712797.2 , XM_011511991.2
Accession Version XM_011511991.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1143bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-03-12
Organism Homo sapiens(Human)
Product cytoplasmic protein NCK2 isoform X1
Comment 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGACAGAAG AAGTTATTGT GATAGCCAAG TGGGACTACA CCGCCCAGCA GGACCAGGAG 
CTGGACATCA AGAAGAACGA GCGGCTGTGG TTGCTGGACG ACTCCAAGAC GTGGTGGCGG 
GTGAGGAACG CGGCCAACAG GACGGGCTAT GTACCGTCCA ACTACGTGGA GCGGAAGAAC 
AGCCTGAAGA AGGGCTCCCT CGTGAAGAAC CTGAAGGACA CACTAGGCCT CGGCAAGACG 
CGCAGGAAGA CCAGCGCGCG GGATGCGTCC CCCACGCCCA GCACGGACGC CGAGTACCCC 
GCCAATGGCA GCGGCGCCGA CCGCATCTAC GACCTCAACA TCCCGGCCTT CGTCAAGTTC 
GCCTATGTGG CCGAGCGGGA GGATGAGTTG TCCCTGGTGA AGGGGTCGCG CGTCACCGTC 
ATGGAGAAGT GCAGCGACGG TTGGTGGCGG GGCAGCTACA ACGGGCAGAT CGGCTGGTTC 
CCCTCCAACT ACGTCTTGGA GGAGGTGGAC GAGGCGGCTG CGGAGTCCCC AAGCTTCCTG 
AGCCTGCGCA AGGGCGCCTC GCTGAGCAAT GGCCAGGGCT CCCGCGTGCT GCATGTGGTC 
CAGACGCTGT ACCCCTTCAG CTCAGTCACC GAGGAGGAGC TCAACTTCGA GAAGGGGGAG 
ACCATGGAGG TGATTGAGAA GCCGGAGAAC GACCCCGAGT GGTGGAAATG CAAAAATGCC 
CGGGGCCAGG TGGGCCTCGT CCCCAAAAAC TACGTGGTGG TCCTCAGTGA CGGGCCTGCC 
CTGCACCCTG CGCACGCCCC ACAGATAAGC TACACCGGGC CCTCGTCCAG CGGGCGCTTC 
GCGGGCAGAG AGTGGTACTA CGGGAACGTG ACGCGGCACC AGGCCGAGTG CGCCCTCAAC 
GAGCGGGGCG TGGAGGGCGA CTTCCTCATT AGGGACAGCG AGTCCTCGCC CAGCGACTTC 
TCCGTGTCCC TTAAAGCGTC AGGGAAGAAC AAACACTTCA AGGTGCAGCT CGTGGACAAT 
GTCTACTGCA TTGGGCAGCG GCGCTTCCAC ACCATGGACG AGCTGGTGGA ACACTACAAA 
AAGGCGCCCA TCTTCACCAG CGAGCACGGG GAGAAGCTCT ACCTCGTCAG GGCCCTGCAG 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011510293.1
Translation

Target ORF information:

RefSeq Version XM_011511991.1
Organism Homo sapiens(Human)
Definition Homo sapiens NCK adaptor protein 2 (NCK2), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011511991.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGACAGAAG AAGTTATTGT GATAGCCAAG TGGGACTACA CCGCCCAGCA GGACCAGGAG 
CTGGACATCA AGAAGAACGA GCGGCTGTGG TTGCTGGACG ACTCCAAGAC GTGGTGGCGG 
GTGAGGAACG CGGCCAACAG GACGGGCTAT GTACCGTCCA ACTACGTGGA GCGGAAGAAC 
AGCCTGAAGA AGGGCTCCCT CGTGAAGAAC CTGAAGGACA CACTAGGCCT CGGCAAGACG 
CGCAGGAAGA CCAGCGCGCG GGATGCGTCC CCCACGCCCA GCACGGACGC CGAGTACCCC 
GCCAATGGCA GCGGCGCCGA CCGCATCTAC GACCTCAACA TCCCGGCCTT CGTCAAGTTC 
GCCTATGTGG CCGAGCGGGA GGATGAGTTG TCCCTGGTGA AGGGGTCGCG CGTCACCGTC 
ATGGAGAAGT GCAGCGACGG TTGGTGGCGG GGCAGCTACA ACGGGCAGAT CGGCTGGTTC 
CCCTCCAACT ACGTCTTGGA GGAGGTGGAC GAGGCGGCTG CGGAGTCCCC AAGCTTCCTG 
AGCCTGCGCA AGGGCGCCTC GCTGAGCAAT GGCCAGGGCT CCCGCGTGCT GCATGTGGTC 
CAGACGCTGT ACCCCTTCAG CTCAGTCACC GAGGAGGAGC TCAACTTCGA GAAGGGGGAG 
ACCATGGAGG TGATTGAGAA GCCGGAGAAC GACCCCGAGT GGTGGAAATG CAAAAATGCC 
CGGGGCCAGG TGGGCCTCGT CCCCAAAAAC TACGTGGTGG TCCTCAGTGA CGGGCCTGCC 
CTGCACCCTG CGCACGCCCC ACAGATAAGC TACACCGGGC CCTCGTCCAG CGGGCGCTTC 
GCGGGCAGAG AGTGGTACTA CGGGAACGTG ACGCGGCACC AGGCCGAGTG CGCCCTCAAC 
GAGCGGGGCG TGGAGGGCGA CTTCCTCATT AGGGACAGCG AGTCCTCGCC CAGCGACTTC 
TCCGTGTCCC TTAAAGCGTC AGGGAAGAAC AAACACTTCA AGGTGCAGCT CGTGGACAAT 
GTCTACTGCA TTGGGCAGCG GCGCTTCCAC ACCATGGACG AGCTGGTGGA ACACTACAAA 
AAGGCGCCCA TCTTCACCAG CGAGCACGGG GAGAAGCTCT ACCTCGTCAG GGCCCTGCAG 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu14522
Clone ID Related Accession (Same CDS sequence) XM_017005104.1 , XM_017005105.1 , NM_001004720.2 , XM_011511991.1 , XM_017005103.1 , XM_006712797.3 , NM_003581.4 , XM_006712797.2 , XM_011511991.2
Accession Version XM_017005103.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1143bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product cytoplasmic protein NCK2 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGACAGAAG AAGTTATTGT GATAGCCAAG TGGGACTACA CCGCCCAGCA GGACCAGGAG 
CTGGACATCA AGAAGAACGA GCGGCTGTGG TTGCTGGACG ACTCCAAGAC GTGGTGGCGG 
GTGAGGAACG CGGCCAACAG GACGGGCTAT GTACCGTCCA ACTACGTGGA GCGGAAGAAC 
AGCCTGAAGA AGGGCTCCCT CGTGAAGAAC CTGAAGGACA CACTAGGCCT CGGCAAGACG 
CGCAGGAAGA CCAGCGCGCG GGATGCGTCC CCCACGCCCA GCACGGACGC CGAGTACCCC 
GCCAATGGCA GCGGCGCCGA CCGCATCTAC GACCTCAACA TCCCGGCCTT CGTCAAGTTC 
GCCTATGTGG CCGAGCGGGA GGATGAGTTG TCCCTGGTGA AGGGGTCGCG CGTCACCGTC 
ATGGAGAAGT GCAGCGACGG TTGGTGGCGG GGCAGCTACA ACGGGCAGAT CGGCTGGTTC 
CCCTCCAACT ACGTCTTGGA GGAGGTGGAC GAGGCGGCTG CGGAGTCCCC AAGCTTCCTG 
AGCCTGCGCA AGGGCGCCTC GCTGAGCAAT GGCCAGGGCT CCCGCGTGCT GCATGTGGTC 
CAGACGCTGT ACCCCTTCAG CTCAGTCACC GAGGAGGAGC TCAACTTCGA GAAGGGGGAG 
ACCATGGAGG TGATTGAGAA GCCGGAGAAC GACCCCGAGT GGTGGAAATG CAAAAATGCC 
CGGGGCCAGG TGGGCCTCGT CCCCAAAAAC TACGTGGTGG TCCTCAGTGA CGGGCCTGCC 
CTGCACCCTG CGCACGCCCC ACAGATAAGC TACACCGGGC CCTCGTCCAG CGGGCGCTTC 
GCGGGCAGAG AGTGGTACTA CGGGAACGTG ACGCGGCACC AGGCCGAGTG CGCCCTCAAC 
GAGCGGGGCG TGGAGGGCGA CTTCCTCATT AGGGACAGCG AGTCCTCGCC CAGCGACTTC 
TCCGTGTCCC TTAAAGCGTC AGGGAAGAAC AAACACTTCA AGGTGCAGCT CGTGGACAAT 
GTCTACTGCA TTGGGCAGCG GCGCTTCCAC ACCATGGACG AGCTGGTGGA ACACTACAAA 
AAGGCGCCCA TCTTCACCAG CGAGCACGGG GAGAAGCTCT ACCTCGTCAG GGCCCTGCAG 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016860592.1
CDS535..1677
Misc Feature(1)538..711
Misc Feature(2)order(571..573,580..585,589..594,640..648,682..684,688..699)
Misc Feature(3)874..1038
Misc Feature(4)order(892..894,898..900,907..909,919..921,973..978,1015..1017,1021..1026)
Misc Feature(5)1126..1302
Misc Feature(6)order(1144..1146,1150..1152,1159..1161,1171..1173,1231..1236,1276..1278,1282..1287)
Misc Feature(7)order(1237..1239,1279..1284,1288..1296)
Misc Feature(8)1381..1674
Misc Feature(9)order(1408..1410,1465..1467,1471..1479,1495..1497,1534..1536)
Misc Feature(10)order(1531..1533,1564..1566,1573..1575,1624..1632)
Translation

Target ORF information:

RefSeq Version XM_017005103.1
Organism Homo sapiens(human)
Definition Homo sapiens NCK adaptor protein 2 (NCK2), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017005103.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGACAGAAG AAGTTATTGT GATAGCCAAG TGGGACTACA CCGCCCAGCA GGACCAGGAG 
CTGGACATCA AGAAGAACGA GCGGCTGTGG TTGCTGGACG ACTCCAAGAC GTGGTGGCGG 
GTGAGGAACG CGGCCAACAG GACGGGCTAT GTACCGTCCA ACTACGTGGA GCGGAAGAAC 
AGCCTGAAGA AGGGCTCCCT CGTGAAGAAC CTGAAGGACA CACTAGGCCT CGGCAAGACG 
CGCAGGAAGA CCAGCGCGCG GGATGCGTCC CCCACGCCCA GCACGGACGC CGAGTACCCC 
GCCAATGGCA GCGGCGCCGA CCGCATCTAC GACCTCAACA TCCCGGCCTT CGTCAAGTTC 
GCCTATGTGG CCGAGCGGGA GGATGAGTTG TCCCTGGTGA AGGGGTCGCG CGTCACCGTC 
ATGGAGAAGT GCAGCGACGG TTGGTGGCGG GGCAGCTACA ACGGGCAGAT CGGCTGGTTC 
CCCTCCAACT ACGTCTTGGA GGAGGTGGAC GAGGCGGCTG CGGAGTCCCC AAGCTTCCTG 
AGCCTGCGCA AGGGCGCCTC GCTGAGCAAT GGCCAGGGCT CCCGCGTGCT GCATGTGGTC 
CAGACGCTGT ACCCCTTCAG CTCAGTCACC GAGGAGGAGC TCAACTTCGA GAAGGGGGAG 
ACCATGGAGG TGATTGAGAA GCCGGAGAAC GACCCCGAGT GGTGGAAATG CAAAAATGCC 
CGGGGCCAGG TGGGCCTCGT CCCCAAAAAC TACGTGGTGG TCCTCAGTGA CGGGCCTGCC 
CTGCACCCTG CGCACGCCCC ACAGATAAGC TACACCGGGC CCTCGTCCAG CGGGCGCTTC 
GCGGGCAGAG AGTGGTACTA CGGGAACGTG ACGCGGCACC AGGCCGAGTG CGCCCTCAAC 
GAGCGGGGCG TGGAGGGCGA CTTCCTCATT AGGGACAGCG AGTCCTCGCC CAGCGACTTC 
TCCGTGTCCC TTAAAGCGTC AGGGAAGAAC AAACACTTCA AGGTGCAGCT CGTGGACAAT 
GTCTACTGCA TTGGGCAGCG GCGCTTCCAC ACCATGGACG AGCTGGTGGA ACACTACAAA 
AAGGCGCCCA TCTTCACCAG CGAGCACGGG GAGAAGCTCT ACCTCGTCAG GGCCCTGCAG 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu14522
Clone ID Related Accession (Same CDS sequence) XM_017005104.1 , XM_017005105.1 , NM_001004720.2 , XM_011511991.1 , XM_017005103.1 , XM_006712797.3 , NM_003581.4 , XM_006712797.2 , XM_011511991.2
Accession Version XM_006712797.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1143bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product cytoplasmic protein NCK2 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006712797.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGACAGAAG AAGTTATTGT GATAGCCAAG TGGGACTACA CCGCCCAGCA GGACCAGGAG 
CTGGACATCA AGAAGAACGA GCGGCTGTGG TTGCTGGACG ACTCCAAGAC GTGGTGGCGG 
GTGAGGAACG CGGCCAACAG GACGGGCTAT GTACCGTCCA ACTACGTGGA GCGGAAGAAC 
AGCCTGAAGA AGGGCTCCCT CGTGAAGAAC CTGAAGGACA CACTAGGCCT CGGCAAGACG 
CGCAGGAAGA CCAGCGCGCG GGATGCGTCC CCCACGCCCA GCACGGACGC CGAGTACCCC 
GCCAATGGCA GCGGCGCCGA CCGCATCTAC GACCTCAACA TCCCGGCCTT CGTCAAGTTC 
GCCTATGTGG CCGAGCGGGA GGATGAGTTG TCCCTGGTGA AGGGGTCGCG CGTCACCGTC 
ATGGAGAAGT GCAGCGACGG TTGGTGGCGG GGCAGCTACA ACGGGCAGAT CGGCTGGTTC 
CCCTCCAACT ACGTCTTGGA GGAGGTGGAC GAGGCGGCTG CGGAGTCCCC AAGCTTCCTG 
AGCCTGCGCA AGGGCGCCTC GCTGAGCAAT GGCCAGGGCT CCCGCGTGCT GCATGTGGTC 
CAGACGCTGT ACCCCTTCAG CTCAGTCACC GAGGAGGAGC TCAACTTCGA GAAGGGGGAG 
ACCATGGAGG TGATTGAGAA GCCGGAGAAC GACCCCGAGT GGTGGAAATG CAAAAATGCC 
CGGGGCCAGG TGGGCCTCGT CCCCAAAAAC TACGTGGTGG TCCTCAGTGA CGGGCCTGCC 
CTGCACCCTG CGCACGCCCC ACAGATAAGC TACACCGGGC CCTCGTCCAG CGGGCGCTTC 
GCGGGCAGAG AGTGGTACTA CGGGAACGTG ACGCGGCACC AGGCCGAGTG CGCCCTCAAC 
GAGCGGGGCG TGGAGGGCGA CTTCCTCATT AGGGACAGCG AGTCCTCGCC CAGCGACTTC 
TCCGTGTCCC TTAAAGCGTC AGGGAAGAAC AAACACTTCA AGGTGCAGCT CGTGGACAAT 
GTCTACTGCA TTGGGCAGCG GCGCTTCCAC ACCATGGACG AGCTGGTGGA ACACTACAAA 
AAGGCGCCCA TCTTCACCAG CGAGCACGGG GAGAAGCTCT ACCTCGTCAG GGCCCTGCAG 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006712860.1
CDS933..2075
Misc Feature(1)936..1109
Misc Feature(2)order(969..971,978..983,987..992,1038..1046,1080..1082,1086..1097)
Misc Feature(3)1272..1436
Misc Feature(4)order(1290..1292,1296..1298,1305..1307,1317..1319,1371..1376,1413..1415,1419..1424)
Misc Feature(5)1524..1700
Misc Feature(6)order(1542..1544,1548..1550,1557..1559,1569..1571,1629..1634,1674..1676,1680..1685)
Misc Feature(7)order(1635..1637,1677..1682,1686..1694)
Misc Feature(8)1779..2072
Misc Feature(9)order(1806..1808,1863..1865,1869..1877,1893..1895,1932..1934)
Misc Feature(10)order(1929..1931,1962..1964,1971..1973,2022..2030)
Translation

Target ORF information:

RefSeq Version XM_006712797.3
Organism Homo sapiens(human)
Definition Homo sapiens NCK adaptor protein 2 (NCK2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006712797.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGACAGAAG AAGTTATTGT GATAGCCAAG TGGGACTACA CCGCCCAGCA GGACCAGGAG 
CTGGACATCA AGAAGAACGA GCGGCTGTGG TTGCTGGACG ACTCCAAGAC GTGGTGGCGG 
GTGAGGAACG CGGCCAACAG GACGGGCTAT GTACCGTCCA ACTACGTGGA GCGGAAGAAC 
AGCCTGAAGA AGGGCTCCCT CGTGAAGAAC CTGAAGGACA CACTAGGCCT CGGCAAGACG 
CGCAGGAAGA CCAGCGCGCG GGATGCGTCC CCCACGCCCA GCACGGACGC CGAGTACCCC 
GCCAATGGCA GCGGCGCCGA CCGCATCTAC GACCTCAACA TCCCGGCCTT CGTCAAGTTC 
GCCTATGTGG CCGAGCGGGA GGATGAGTTG TCCCTGGTGA AGGGGTCGCG CGTCACCGTC 
ATGGAGAAGT GCAGCGACGG TTGGTGGCGG GGCAGCTACA ACGGGCAGAT CGGCTGGTTC 
CCCTCCAACT ACGTCTTGGA GGAGGTGGAC GAGGCGGCTG CGGAGTCCCC AAGCTTCCTG 
AGCCTGCGCA AGGGCGCCTC GCTGAGCAAT GGCCAGGGCT CCCGCGTGCT GCATGTGGTC 
CAGACGCTGT ACCCCTTCAG CTCAGTCACC GAGGAGGAGC TCAACTTCGA GAAGGGGGAG 
ACCATGGAGG TGATTGAGAA GCCGGAGAAC GACCCCGAGT GGTGGAAATG CAAAAATGCC 
CGGGGCCAGG TGGGCCTCGT CCCCAAAAAC TACGTGGTGG TCCTCAGTGA CGGGCCTGCC 
CTGCACCCTG CGCACGCCCC ACAGATAAGC TACACCGGGC CCTCGTCCAG CGGGCGCTTC 
GCGGGCAGAG AGTGGTACTA CGGGAACGTG ACGCGGCACC AGGCCGAGTG CGCCCTCAAC 
GAGCGGGGCG TGGAGGGCGA CTTCCTCATT AGGGACAGCG AGTCCTCGCC CAGCGACTTC 
TCCGTGTCCC TTAAAGCGTC AGGGAAGAAC AAACACTTCA AGGTGCAGCT CGTGGACAAT 
GTCTACTGCA TTGGGCAGCG GCGCTTCCAC ACCATGGACG AGCTGGTGGA ACACTACAAA 
AAGGCGCCCA TCTTCACCAG CGAGCACGGG GAGAAGCTCT ACCTCGTCAG GGCCCTGCAG 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu14522
Clone ID Related Accession (Same CDS sequence) XM_017005104.1 , XM_017005105.1 , NM_001004720.2 , XM_011511991.1 , XM_017005103.1 , XM_006712797.3 , NM_003581.4 , XM_006712797.2 , XM_011511991.2
Accession Version XM_006712797.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1143bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-03-12
Organism Homo sapiens(Human)
Product cytoplasmic protein NCK2 isoform X1
Comment 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGACAGAAG AAGTTATTGT GATAGCCAAG TGGGACTACA CCGCCCAGCA GGACCAGGAG 
CTGGACATCA AGAAGAACGA GCGGCTGTGG TTGCTGGACG ACTCCAAGAC GTGGTGGCGG 
GTGAGGAACG CGGCCAACAG GACGGGCTAT GTACCGTCCA ACTACGTGGA GCGGAAGAAC 
AGCCTGAAGA AGGGCTCCCT CGTGAAGAAC CTGAAGGACA CACTAGGCCT CGGCAAGACG 
CGCAGGAAGA CCAGCGCGCG GGATGCGTCC CCCACGCCCA GCACGGACGC CGAGTACCCC 
GCCAATGGCA GCGGCGCCGA CCGCATCTAC GACCTCAACA TCCCGGCCTT CGTCAAGTTC 
GCCTATGTGG CCGAGCGGGA GGATGAGTTG TCCCTGGTGA AGGGGTCGCG CGTCACCGTC 
ATGGAGAAGT GCAGCGACGG TTGGTGGCGG GGCAGCTACA ACGGGCAGAT CGGCTGGTTC 
CCCTCCAACT ACGTCTTGGA GGAGGTGGAC GAGGCGGCTG CGGAGTCCCC AAGCTTCCTG 
AGCCTGCGCA AGGGCGCCTC GCTGAGCAAT GGCCAGGGCT CCCGCGTGCT GCATGTGGTC 
CAGACGCTGT ACCCCTTCAG CTCAGTCACC GAGGAGGAGC TCAACTTCGA GAAGGGGGAG 
ACCATGGAGG TGATTGAGAA GCCGGAGAAC GACCCCGAGT GGTGGAAATG CAAAAATGCC 
CGGGGCCAGG TGGGCCTCGT CCCCAAAAAC TACGTGGTGG TCCTCAGTGA CGGGCCTGCC 
CTGCACCCTG CGCACGCCCC ACAGATAAGC TACACCGGGC CCTCGTCCAG CGGGCGCTTC 
GCGGGCAGAG AGTGGTACTA CGGGAACGTG ACGCGGCACC AGGCCGAGTG CGCCCTCAAC 
GAGCGGGGCG TGGAGGGCGA CTTCCTCATT AGGGACAGCG AGTCCTCGCC CAGCGACTTC 
TCCGTGTCCC TTAAAGCGTC AGGGAAGAAC AAACACTTCA AGGTGCAGCT CGTGGACAAT 
GTCTACTGCA TTGGGCAGCG GCGCTTCCAC ACCATGGACG AGCTGGTGGA ACACTACAAA 
AAGGCGCCCA TCTTCACCAG CGAGCACGGG GAGAAGCTCT ACCTCGTCAG GGCCCTGCAG 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006712860.1
Translation

Target ORF information:

RefSeq Version XM_006712797.2
Organism Homo sapiens(Human)
Definition Homo sapiens NCK adaptor protein 2 (NCK2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006712797.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGACAGAAG AAGTTATTGT GATAGCCAAG TGGGACTACA CCGCCCAGCA GGACCAGGAG 
CTGGACATCA AGAAGAACGA GCGGCTGTGG TTGCTGGACG ACTCCAAGAC GTGGTGGCGG 
GTGAGGAACG CGGCCAACAG GACGGGCTAT GTACCGTCCA ACTACGTGGA GCGGAAGAAC 
AGCCTGAAGA AGGGCTCCCT CGTGAAGAAC CTGAAGGACA CACTAGGCCT CGGCAAGACG 
CGCAGGAAGA CCAGCGCGCG GGATGCGTCC CCCACGCCCA GCACGGACGC CGAGTACCCC 
GCCAATGGCA GCGGCGCCGA CCGCATCTAC GACCTCAACA TCCCGGCCTT CGTCAAGTTC 
GCCTATGTGG CCGAGCGGGA GGATGAGTTG TCCCTGGTGA AGGGGTCGCG CGTCACCGTC 
ATGGAGAAGT GCAGCGACGG TTGGTGGCGG GGCAGCTACA ACGGGCAGAT CGGCTGGTTC 
CCCTCCAACT ACGTCTTGGA GGAGGTGGAC GAGGCGGCTG CGGAGTCCCC AAGCTTCCTG 
AGCCTGCGCA AGGGCGCCTC GCTGAGCAAT GGCCAGGGCT CCCGCGTGCT GCATGTGGTC 
CAGACGCTGT ACCCCTTCAG CTCAGTCACC GAGGAGGAGC TCAACTTCGA GAAGGGGGAG 
ACCATGGAGG TGATTGAGAA GCCGGAGAAC GACCCCGAGT GGTGGAAATG CAAAAATGCC 
CGGGGCCAGG TGGGCCTCGT CCCCAAAAAC TACGTGGTGG TCCTCAGTGA CGGGCCTGCC 
CTGCACCCTG CGCACGCCCC ACAGATAAGC TACACCGGGC CCTCGTCCAG CGGGCGCTTC 
GCGGGCAGAG AGTGGTACTA CGGGAACGTG ACGCGGCACC AGGCCGAGTG CGCCCTCAAC 
GAGCGGGGCG TGGAGGGCGA CTTCCTCATT AGGGACAGCG AGTCCTCGCC CAGCGACTTC 
TCCGTGTCCC TTAAAGCGTC AGGGAAGAAC AAACACTTCA AGGTGCAGCT CGTGGACAAT 
GTCTACTGCA TTGGGCAGCG GCGCTTCCAC ACCATGGACG AGCTGGTGGA ACACTACAAA 
AAGGCGCCCA TCTTCACCAG CGAGCACGGG GAGAAGCTCT ACCTCGTCAG GGCCCTGCAG 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu14522
Clone ID Related Accession (Same CDS sequence) XM_017005104.1 , XM_017005105.1 , NM_001004720.2 , XM_011511991.1 , XM_017005103.1 , XM_006712797.3 , NM_003581.4 , XM_006712797.2 , XM_011511991.2
Accession Version XM_011511991.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1143bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product cytoplasmic protein NCK2 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011511991.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGACAGAAG AAGTTATTGT GATAGCCAAG TGGGACTACA CCGCCCAGCA GGACCAGGAG 
CTGGACATCA AGAAGAACGA GCGGCTGTGG TTGCTGGACG ACTCCAAGAC GTGGTGGCGG 
GTGAGGAACG CGGCCAACAG GACGGGCTAT GTACCGTCCA ACTACGTGGA GCGGAAGAAC 
AGCCTGAAGA AGGGCTCCCT CGTGAAGAAC CTGAAGGACA CACTAGGCCT CGGCAAGACG 
CGCAGGAAGA CCAGCGCGCG GGATGCGTCC CCCACGCCCA GCACGGACGC CGAGTACCCC 
GCCAATGGCA GCGGCGCCGA CCGCATCTAC GACCTCAACA TCCCGGCCTT CGTCAAGTTC 
GCCTATGTGG CCGAGCGGGA GGATGAGTTG TCCCTGGTGA AGGGGTCGCG CGTCACCGTC 
ATGGAGAAGT GCAGCGACGG TTGGTGGCGG GGCAGCTACA ACGGGCAGAT CGGCTGGTTC 
CCCTCCAACT ACGTCTTGGA GGAGGTGGAC GAGGCGGCTG CGGAGTCCCC AAGCTTCCTG 
AGCCTGCGCA AGGGCGCCTC GCTGAGCAAT GGCCAGGGCT CCCGCGTGCT GCATGTGGTC 
CAGACGCTGT ACCCCTTCAG CTCAGTCACC GAGGAGGAGC TCAACTTCGA GAAGGGGGAG 
ACCATGGAGG TGATTGAGAA GCCGGAGAAC GACCCCGAGT GGTGGAAATG CAAAAATGCC 
CGGGGCCAGG TGGGCCTCGT CCCCAAAAAC TACGTGGTGG TCCTCAGTGA CGGGCCTGCC 
CTGCACCCTG CGCACGCCCC ACAGATAAGC TACACCGGGC CCTCGTCCAG CGGGCGCTTC 
GCGGGCAGAG AGTGGTACTA CGGGAACGTG ACGCGGCACC AGGCCGAGTG CGCCCTCAAC 
GAGCGGGGCG TGGAGGGCGA CTTCCTCATT AGGGACAGCG AGTCCTCGCC CAGCGACTTC 
TCCGTGTCCC TTAAAGCGTC AGGGAAGAAC AAACACTTCA AGGTGCAGCT CGTGGACAAT 
GTCTACTGCA TTGGGCAGCG GCGCTTCCAC ACCATGGACG AGCTGGTGGA ACACTACAAA 
AAGGCGCCCA TCTTCACCAG CGAGCACGGG GAGAAGCTCT ACCTCGTCAG GGCCCTGCAG 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011510293.1
CDS371..1513
Misc Feature(1)374..547
Misc Feature(2)order(407..409,416..421,425..430,476..484,518..520,524..535)
Misc Feature(3)710..874
Misc Feature(4)order(728..730,734..736,743..745,755..757,809..814,851..853,857..862)
Misc Feature(5)962..1138
Misc Feature(6)order(980..982,986..988,995..997,1007..1009,1067..1072,1112..1114,1118..1123)
Misc Feature(7)order(1073..1075,1115..1120,1124..1132)
Misc Feature(8)1217..1510
Misc Feature(9)order(1244..1246,1301..1303,1307..1315,1331..1333,1370..1372)
Misc Feature(10)order(1367..1369,1400..1402,1409..1411,1460..1468)
Translation

Target ORF information:

RefSeq Version XM_011511991.2
Organism Homo sapiens(human)
Definition Homo sapiens NCK adaptor protein 2 (NCK2), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011511991.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGACAGAAG AAGTTATTGT GATAGCCAAG TGGGACTACA CCGCCCAGCA GGACCAGGAG 
CTGGACATCA AGAAGAACGA GCGGCTGTGG TTGCTGGACG ACTCCAAGAC GTGGTGGCGG 
GTGAGGAACG CGGCCAACAG GACGGGCTAT GTACCGTCCA ACTACGTGGA GCGGAAGAAC 
AGCCTGAAGA AGGGCTCCCT CGTGAAGAAC CTGAAGGACA CACTAGGCCT CGGCAAGACG 
CGCAGGAAGA CCAGCGCGCG GGATGCGTCC CCCACGCCCA GCACGGACGC CGAGTACCCC 
GCCAATGGCA GCGGCGCCGA CCGCATCTAC GACCTCAACA TCCCGGCCTT CGTCAAGTTC 
GCCTATGTGG CCGAGCGGGA GGATGAGTTG TCCCTGGTGA AGGGGTCGCG CGTCACCGTC 
ATGGAGAAGT GCAGCGACGG TTGGTGGCGG GGCAGCTACA ACGGGCAGAT CGGCTGGTTC 
CCCTCCAACT ACGTCTTGGA GGAGGTGGAC GAGGCGGCTG CGGAGTCCCC AAGCTTCCTG 
AGCCTGCGCA AGGGCGCCTC GCTGAGCAAT GGCCAGGGCT CCCGCGTGCT GCATGTGGTC 
CAGACGCTGT ACCCCTTCAG CTCAGTCACC GAGGAGGAGC TCAACTTCGA GAAGGGGGAG 
ACCATGGAGG TGATTGAGAA GCCGGAGAAC GACCCCGAGT GGTGGAAATG CAAAAATGCC 
CGGGGCCAGG TGGGCCTCGT CCCCAAAAAC TACGTGGTGG TCCTCAGTGA CGGGCCTGCC 
CTGCACCCTG CGCACGCCCC ACAGATAAGC TACACCGGGC CCTCGTCCAG CGGGCGCTTC 
GCGGGCAGAG AGTGGTACTA CGGGAACGTG ACGCGGCACC AGGCCGAGTG CGCCCTCAAC 
GAGCGGGGCG TGGAGGGCGA CTTCCTCATT AGGGACAGCG AGTCCTCGCC CAGCGACTTC 
TCCGTGTCCC TTAAAGCGTC AGGGAAGAAC AAACACTTCA AGGTGCAGCT CGTGGACAAT 
GTCTACTGCA TTGGGCAGCG GCGCTTCCAC ACCATGGACG AGCTGGTGGA ACACTACAAA 
AAGGCGCCCA TCTTCACCAG CGAGCACGGG GAGAAGCTCT ACCTCGTCAG GGCCCTGCAG 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu14299
Clone ID Related Accession (Same CDS sequence) XM_011511992.2 , NM_001004722.3
Accession Version NM_001004722.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 252bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-10-03
Organism Homo sapiens(human)
Product cytoplasmic protein NCK2 isoform B
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010978.8, BG480643.1 and AC009505.3. On Jul 30, 2011 this sequence version replaced NM_001004722.2. Transcript Variant: This variant (3) lacks an exon, which results in a frameshift, compared to variant 1. The resulting protein (isoform B) is shorter and has a distinct C-terminus, compared to isoform A. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: ERR279856.4275.1, BQ684659.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
ATGACAGAAG AAGTTATTGT GATAGCCAAG TGGGACTACA CCGCCCAGCA GGACCAGGAG 
CTGGACATCA AGAAGAACGA GCGGCTGTGG TTGCTGGACG ACTCCAAGAC GTGGTGGCGG 
GTGAGGAACG CGGCCAACAG GACGGGCTAT GTACCGTCCA ACTACGTGGA GCGGAAGAAC 
AGCCTGAAGA AGGGCTCCCT CGTGAAGAAC CTGAAGGACA CACTAGCCCA GCGACTTCTC 
CGTGTCCCTT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001004722.1
CDS277..528
Misc Feature(1)271..273
Misc Feature(2)280..453
Misc Feature(3)280..282
Misc Feature(4)order(307..309,313..315,322..324,334..336,385..390,430..432,436..441)
Exon (1)1..76
Exon (2)77..260
Exon (3)261..502
Exon (4)503..1795
Translation

Target ORF information:

RefSeq Version NM_001004722.3
Organism Homo sapiens(human)
Definition Homo sapiens NCK adaptor protein 2 (NCK2), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001004722.3

ORF Insert Sequence:

1
61
121
181
241
ATGACAGAAG AAGTTATTGT GATAGCCAAG TGGGACTACA CCGCCCAGCA GGACCAGGAG 
CTGGACATCA AGAAGAACGA GCGGCTGTGG TTGCTGGACG ACTCCAAGAC GTGGTGGCGG 
GTGAGGAACG CGGCCAACAG GACGGGCTAT GTACCGTCCA ACTACGTGGA GCGGAAGAAC 
AGCCTGAAGA AGGGCTCCCT CGTGAAGAAC CTGAAGGACA CACTAGCCCA GCGACTTCTC 
CGTGTCCCTT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu14299
Clone ID Related Accession (Same CDS sequence) XM_011511992.2 , NM_001004722.3
Accession Version XM_011511992.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 252bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product cytoplasmic protein NCK2 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011511992.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
ATGACAGAAG AAGTTATTGT GATAGCCAAG TGGGACTACA CCGCCCAGCA GGACCAGGAG 
CTGGACATCA AGAAGAACGA GCGGCTGTGG TTGCTGGACG ACTCCAAGAC GTGGTGGCGG 
GTGAGGAACG CGGCCAACAG GACGGGCTAT GTACCGTCCA ACTACGTGGA GCGGAAGAAC 
AGCCTGAAGA AGGGCTCCCT CGTGAAGAAC CTGAAGGACA CACTAGCCCA GCGACTTCTC 
CGTGTCCCTT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011510294.2
CDS221..472
Misc Feature(1)224..397
Misc Feature(2)order(251..253,257..259,266..268,278..280,329..334,374..376,380..385)
Translation

Target ORF information:

RefSeq Version XM_011511992.2
Organism Homo sapiens(human)
Definition Homo sapiens NCK adaptor protein 2 (NCK2), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011511992.2

ORF Insert Sequence:

1
61
121
181
241
ATGACAGAAG AAGTTATTGT GATAGCCAAG TGGGACTACA CCGCCCAGCA GGACCAGGAG 
CTGGACATCA AGAAGAACGA GCGGCTGTGG TTGCTGGACG ACTCCAAGAC GTGGTGGCGG 
GTGAGGAACG CGGCCAACAG GACGGGCTAT GTACCGTCCA ACTACGTGGA GCGGAAGAAC 
AGCCTGAAGA AGGGCTCCCT CGTGAAGAAC CTGAAGGACA CACTAGCCCA GCGACTTCTC 
CGTGTCCCTT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Nephrin Suppresses Hippo Signaling through the Adaptor Proteins Nck and WTIP.
The Journal of biological chemistry291(24)12799-808(2016 Jun)
Keyvani Chahi A,Martin CE,Jones N


book

An inter-species protein-protein interaction network across vast evolutionary distance.
Molecular systems biology12(4)865(2016 Apr)
Zhong Q,Pevzner SJ,Hao T,Wang Y,Mosca R,Menche J,Taipale M,Ta?an M,Fan C,Yang X,Haley P,Murray RR,Mer F,Gebreab F,Tam S,MacWilliams A,Dricot A,Reichert P,Santhanam B,Ghamsari L,Calderwood MA,Rolland T,Charloteaux B,Lindquist S,Barab?si AL,Hill DE,Aloy P,Cusick ME,Xia Y,Roth FP,Vidal M


book

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Cell164(4)805-17(2016 Feb)
Yang X,Coulombe-Huntington J,Kang S,Sheynkman GM,Hao T,Richardson A,Sun S,Yang F,Shen YA,Murray RR,Spirohn K,Begg BE,Duran-Frigola M,MacWilliams A,Pevzner SJ,Zhong Q,Trigg SA,Tam S,Ghamsari L,Sahni N,Yi S,Rodriguez MD,Balcha D,Tan G,Costanzo M,Andrews B,Boone C,Zhou XJ,Salehi-Ashtiani K,Charloteaux B,Chen AA,Calderwood MA,Aloy P,Roth FP,Hill DE,Iakoucheva LM,Xia Y,Vidal M


book

Targeting of the EGFR/?1 integrin connecting proteins PINCH1 and Nck2 radiosensitizes three-dimensional SCC cell cultures.
Oncology reports34(1)469-76(2015 Jul)
Rossow L,Eke I,Dickreuter E,Cordes N


book

Solution structure of the first SRC homology 3 domain of human Nck2.
Journal of biomolecular NMR34(3)203-8(2006 Mar)
Park S,Takeuchi K,Wagner G