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TRMT10A cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol TRMT10A
Entrez Gene ID 93587
Full Name tRNA methyltransferase 10A
Synonyms HEL-S-88,MSSGM,MSSGM1,RG9MTD2,TRM10
General protein information
Preferred Names

tRNA methyltransferase 10A

Names

tRNA methyltransferase 10 homolog A
RNA (guanine-9-)-methyltransferase domain-containing protein 2
epididymis secretory protein Li 88
tRNA (guanine(9)-N(1))-methyltransferase TRMT10A
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

4

4q23
Summary This gene encodes a protein that belongs to the tRNA (Guanine-1)-methyltransferase family. A similar gene in yeast modifies several different tRNA species. Mutations in this gene are associated with microcephaly, short stature, and impaired glucose metabolism. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014].
Disorder MIM:

616013

Disorder Html: Microcephaly, short stature, and impaired glucose metabolism 1, 616033 (3)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001134665.2 NP_001128137.1 tRNA methyltransferase 10 homolog A
NM_001134665.3 NP_001128137.1 tRNA methyltransferase 10 homolog A isoform 1
XM_005263352.3 XP_005263409.1 tRNA methyltransferase 10 homolog A isoform X1
NM_152292.4 NP_689505.1 tRNA methyltransferase 10 homolog A
XM_006714417.2 XP_006714480.1 tRNA methyltransferase 10 homolog A isoform X1
NM_001375881.1 NP_001362810.1 tRNA methyltransferase 10 homolog A isoform 1
NM_001375882.1 NP_001362811.1 tRNA methyltransferase 10 homolog A isoform 2
NM_001134666.3 NP_001128138.1 tRNA methyltransferase 10 homolog A isoform 1
NM_001375880.1 NP_001362809.1 tRNA methyltransferase 10 homolog A isoform 1
NM_152292.5 NP_689505.1 tRNA methyltransferase 10 homolog A isoform 1
NM_001134666.2 NP_001128138.1 tRNA methyltransferase 10 homolog A

Pathways from BioSystems


Homologies


Homo sapiens (human) TRMT10A NP_689505.1
LOC708751 XP_001107346.2
Drosophila melanogaster (fruit fly) CG14618 NP_608464.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP000324 XP_310794.4
Kluyveromyces lactis KLLA0C03740g XP_452363.1
Neurospora crassa NCU02231 XP_959644.1
Canis lupus familiaris (dog) TRMT10A XP_005639279.1
Mus musculus (house mouse) Trmt10a NP_780598.2
Danio rerio (zebrafish) LOC560222 XP_688707.2
Saccharomyces cerevisiae (baker's yeast) TRM10 NP_014548.1
Pan troglodytes (chimpanzee) TRMT10A XP_001167367.1
Magnaporthe oryzae (rice blast fungus) MGG_00880 XP_003718089.1
Rattus norvegicus (Norway rat) Trmt10a NP_001037697.1
Caenorhabditis elegans (roundworm) F25H8.1 NP_001122773.1
Eremothecium gossypii AGOS_ADR110C NP_984206.1
Schizosaccharomyces pombe (fission yeast) trm10 NP_593764.1
Xenopus tropicalis (tropical clawed frog) trmt10a NP_001008012.1
Bos taurus (cattle) TRMT10A NP_001030554.1
Gallus gallus (chicken) TRMT10A XP_001234301.2

Gene Ontology


Bibliography

Related articles in PubMed

tRNA methyltransferase homologue gene TRMT10A mutation in young adult-onset diabetes with intellectual disability, microcephaly and epilepsy.
Yew TW, McCreight L, Colclough K, Ellard S, Pearson ER
Diabetic medicine : a journal of the British Diabetic Association33(9)e21-5(2016 Sep)

Homozygous deletion of TRMT10A as part of a contiguous gene deletion in a syndrome of failure to thrive, delayed puberty, intellectual disability and diabetes mellitus.
Zung A, Kori M, Burundukov E, Ben-Yosef T, Tatoor Y, Granot E
American journal of medical genetics. Part A167A(12)3167-73(2015 Dec)

TRMT10A dysfunction is associated with abnormalities in glucose homeostasis, short stature and microcephaly.
Gillis D, Krishnamohan A, Yaacov B, Shaag A, Jackman JE, Elpeleg O
Journal of medical genetics51(9)581-6(2014 Sep)

tRNA methyltransferase homolog gene TRMT10A mutation in young onset diabetes and primary microcephaly in humans.
Igoillo-Esteve M, Genin A, Lambert N, D?sir J, Pirson I, Abdulkarim B, Simonis N, Drielsma A, Marselli L, Marchetti P, Vanderhaeghen P, Eizirik DL, Wuyts W, Julier C, Chakera AJ, Ellard S, Hattersley AT, Abramowicz M, Cnop M
PLoS genetics9(10)e1003888(2013 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A homozygous G to T transition in exon 2 of gene TRMT10A at nucleotide position 79 of the coding sequence was identified in a family with young-adult onset diabetes with intellectual disability, microcephaly and epilepsy.
Title: tRNA methyltransferase homologue gene TRMT10A mutation in young adult-onset diabetes with intellectual disability, microcephaly and epilepsy.

Homozygous deletion of TRMT10A is associated with a syndrome of failure to thrive, delayed puberty, intellectual disability and diabetes mellitus.
Title: Homozygous deletion of TRMT10A as part of a contiguous gene deletion in a syndrome of failure to thrive, delayed puberty, intellectual disability and diabetes mellitus.

TRMT10A dysfunction is associated with abnormalities in glucose homeostasis, short stature and microcephaly.
Title: TRMT10A dysfunction is associated with abnormalities in glucose homeostasis, short stature and microcephaly.

This is the first study describing the impact of TRMT10A deficiency in mammals, highlighting a role in the pathogenesis of microcephaly and early onset diabetes.
Title: tRNA methyltransferase homolog gene TRMT10A mutation in young onset diabetes and primary microcephaly in humans.

The following TRMT10A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRMT10A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu24413 NM_152292.5
Latest version!		 Homo sapiens tRNA methyltransferase 10A (TRMT10A), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $159.50-$223.30
$319.00
OHu24413 NM_001375880.1
Latest version!		 Homo sapiens tRNA methyltransferase 10A (TRMT10A), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $159.50-$223.30
$319.00
OHu24413 NM_001375881.1
Latest version!		 Homo sapiens tRNA methyltransferase 10A (TRMT10A), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $159.50-$223.30
$319.00
OHu24413 NM_001134665.3
Latest version!		 Homo sapiens tRNA methyltransferase 10A (TRMT10A), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $159.50-$223.30
$319.00
OHu24413 NM_001134666.3
Latest version!		 Homo sapiens tRNA methyltransferase 10A (TRMT10A), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $159.50-$223.30
$319.00
OHu24413 XM_006714417.2
Latest version!		 Homo sapiens tRNA methyltransferase 10A (TRMT10A), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $159.50-$223.30
$319.00
OHu24413 XM_005263352.3
Latest version!		 Homo sapiens tRNA methyltransferase 10A (TRMT10A), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $159.50-$223.30
$319.00
OHu109021 NM_001375882.1
Latest version!		 Homo sapiens tRNA methyltransferase 10A (TRMT10A), transcript variant 6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $139.30
$199.00
View Old Accession Versions >>
OHu24413 NM_001134665.2 Homo sapiens tRNA methyltransferase 10A (TRMT10A), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $159.50-$223.30
$319.00
OHu24413 NM_152292.4 Homo sapiens tRNA methyltransferase 10A (TRMT10A), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $159.50-$223.30
$319.00
OHu24413 NM_001134666.2 Homo sapiens tRNA methyltransferase 10A (TRMT10A), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $159.50-$223.30
$319.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu24413
Clone ID Related Accession (Same CDS sequence) NM_001134665.2 , NM_001134665.3 , XM_005263352.3 , NM_152292.4 , XM_006714417.2 , NM_001375881.1 , NM_001134666.3 , NM_001375880.1 , NM_152292.5 , NM_001134666.2
Accession Version NM_001134665.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1020bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-12-27
Organism Homo sapiens(human)
Product tRNA methyltransferase 10 homolog A
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB455183.1, AK313548.1, BC028373.2, AC083902.5 and AW242551.1. This sequence is a reference standard in the RefSeqGene project. On Oct 17, 2014 this sequence version replaced NM_001134665.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. All three variants encode the same protein. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.37340.1, SRR1803617.11791.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGTCATCTG AAATGTTGCC AGCATTTATT GAAACTTCTA ATGTTGACAA AAAGCAAGGC 
ATAAATGAAG ATCAAGAGGA GAGCCAGAAG CCAAGATTAG GTGAAGGGTG TGAACCAATA 
TCTAAACGAC AAATGAAAAA ACTAATAAAA CAGAAACAAT GGGAAGAGCA ACGGGAACTC 
CGCAAACAAA AGCGAAAAGA AAAACGCAAG AGGAAAAAAT TAGAGCGACA ATGTCAAATG 
GAACCAAACT CAGATGGACA TGACAGAAAA CGTGTTCGAA GAGATGTTGT TCATAGCACC 
CTTCGCCTTA TTATTGACTG TAGTTTTGAT CACTTGATGG TATTAAAGGA CATTAAGAAA 
CTTCATAAGC AGATTCAACG ATGTTACGCA GAAAACCGAC GGGCACTGCA TCCTGTGCAG 
TTTTACTTGA CAAGCCACGG AGGCCAGCTG AAAAAGAACA TGGATGAAAA TGACAAAGGA 
TGGGTCAACT GGAAGGATAT CCATATCAAA CCAGAGCACT ATAGTGAACT CATAAAGAAA 
GAAGACCTGA TTTACCTTAC GTCAGATTCA CCTAATATAC TGAAGGAATT AGATGAATCA 
AAGGCCTATG TGATTGGAGG ATTAGTAGAT CACAACCATC ACAAGGGACT CACATATAAA 
CAAGCGTCAG ATTATGGAAT CAATCATGCA CAGCTCCCAC TTGGAAATTT TGTGAAGATG 
AATAGTCGAA AAGTTTTGGC AGTTAATCAT GTGTTTGAAA TTATTCTGGA ATACCTGGAA 
ACAAGAGACT GGCAAGAAGC ATTTTTTACT ATCTTGCCCC AACGGAAAGG AGCTGTTCCC 
ACAGACAAAG CCTGTGAAAG TGCTTCTCAT GACAATCAGT CTGTCAGGAT GGAGGAAGGT 
GGATCGGACA GTGATTCCAG TGAGGAGGAA TATAGCAGAA ATGAACTAGA TTCACCACAT 
GAAGAAAAGC AGGATAAGGA AAATCACACT GAATCTACAG TGAACTCTCT GCCACACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001128137.1
CDS169..1188
Translation

Target ORF information:

RefSeq Version NM_001134665.2
Organism Homo sapiens(human)
Definition Homo sapiens tRNA methyltransferase 10A (TRMT10A), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001134665.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGTCATCTG AAATGTTGCC AGCATTTATT GAAACTTCTA ATGTTGACAA AAAGCAAGGC 
ATAAATGAAG ATCAAGAGGA GAGCCAGAAG CCAAGATTAG GTGAAGGGTG TGAACCAATA 
TCTAAACGAC AAATGAAAAA ACTAATAAAA CAGAAACAAT GGGAAGAGCA ACGGGAACTC 
CGCAAACAAA AGCGAAAAGA AAAACGCAAG AGGAAAAAAT TAGAGCGACA ATGTCAAATG 
GAACCAAACT CAGATGGACA TGACAGAAAA CGTGTTCGAA GAGATGTTGT TCATAGCACC 
CTTCGCCTTA TTATTGACTG TAGTTTTGAT CACTTGATGG TATTAAAGGA CATTAAGAAA 
CTTCATAAGC AGATTCAACG ATGTTACGCA GAAAACCGAC GGGCACTGCA TCCTGTGCAG 
TTTTACTTGA CAAGCCACGG AGGCCAGCTG AAAAAGAACA TGGATGAAAA TGACAAAGGA 
TGGGTCAACT GGAAGGATAT CCATATCAAA CCAGAGCACT ATAGTGAACT CATAAAGAAA 
GAAGACCTGA TTTACCTTAC GTCAGATTCA CCTAATATAC TGAAGGAATT AGATGAATCA 
AAGGCCTATG TGATTGGAGG ATTAGTAGAT CACAACCATC ACAAGGGACT CACATATAAA 
CAAGCGTCAG ATTATGGAAT CAATCATGCA CAGCTCCCAC TTGGAAATTT TGTGAAGATG 
AATAGTCGAA AAGTTTTGGC AGTTAATCAT GTGTTTGAAA TTATTCTGGA ATACCTGGAA 
ACAAGAGACT GGCAAGAAGC ATTTTTTACT ATCTTGCCCC AACGGAAAGG AGCTGTTCCC 
ACAGACAAAG CCTGTGAAAG TGCTTCTCAT GACAATCAGT CTGTCAGGAT GGAGGAAGGT 
GGATCGGACA GTGATTCCAG TGAGGAGGAA TATAGCAGAA ATGAACTAGA TTCACCACAT 
GAAGAAAAGC AGGATAAGGA AAATCACACT GAATCTACAG TGAACTCTCT GCCACACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24413
Clone ID Related Accession (Same CDS sequence) NM_001134665.2 , NM_001134665.3 , XM_005263352.3 , NM_152292.4 , XM_006714417.2 , NM_001375881.1 , NM_001134666.3 , NM_001375880.1 , NM_152292.5 , NM_001134666.2
Accession Version NM_001134665.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1020bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-11-04
Organism Homo sapiens(human)
Product tRNA methyltransferase 10 homolog A isoform 1
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB455183.1, AK313548.1, BC028373.2, AC083902.5 and AW242551.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_001134665.2. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.37340.1, SRR1803617.11791.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000394876.7/ ENSP00000378342.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGTCATCTG AAATGTTGCC AGCATTTATT GAAACTTCTA ATGTTGACAA AAAGCAAGGC 
ATAAATGAAG ATCAAGAGGA GAGCCAGAAG CCAAGATTAG GTGAAGGGTG TGAACCAATA 
TCTAAACGAC AAATGAAAAA ACTAATAAAA CAGAAACAAT GGGAAGAGCA ACGGGAACTC 
CGCAAACAAA AGCGAAAAGA AAAACGCAAG AGGAAAAAAT TAGAGCGACA ATGTCAAATG 
GAACCAAACT CAGATGGACA TGACAGAAAA CGTGTTCGAA GAGATGTTGT TCATAGCACC 
CTTCGCCTTA TTATTGACTG TAGTTTTGAT CACTTGATGG TATTAAAGGA CATTAAGAAA 
CTTCATAAGC AGATTCAACG ATGTTACGCA GAAAACCGAC GGGCACTGCA TCCTGTGCAG 
TTTTACTTGA CAAGCCACGG AGGCCAGCTG AAAAAGAACA TGGATGAAAA TGACAAAGGA 
TGGGTCAACT GGAAGGATAT CCATATCAAA CCAGAGCACT ATAGTGAACT CATAAAGAAA 
GAAGACCTGA TTTACCTTAC GTCAGATTCA CCTAATATAC TGAAGGAATT AGATGAATCA 
AAGGCCTATG TGATTGGAGG ATTAGTAGAT CACAACCATC ACAAGGGACT CACATATAAA 
CAAGCGTCAG ATTATGGAAT CAATCATGCA CAGCTCCCAC TTGGAAATTT TGTGAAGATG 
AATAGTCGAA AAGTTTTGGC AGTTAATCAT GTGTTTGAAA TTATTCTGGA ATACCTGGAA 
ACAAGAGACT GGCAAGAAGC ATTTTTTACT ATCTTGCCCC AACGGAAAGG AGCTGTTCCC 
ACAGACAAAG CCTGTGAAAG TGCTTCTCAT GACAATCAGT CTGTCAGGAT GGAGGAAGGT 
GGATCGGACA GTGATTCCAG TGAGGAGGAA TATAGCAGAA ATGAACTAGA TTCACCACAT 
GAAGAAAAGC AGGATAAGGA AAATCACACT GAATCTACAG TGAACTCTCT GCCACACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001128137.1
CDS151..1170
Translation

Target ORF information:

RefSeq Version NM_001134665.3
Organism Homo sapiens(human)
Definition Homo sapiens tRNA methyltransferase 10A (TRMT10A), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001134665.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGTCATCTG AAATGTTGCC AGCATTTATT GAAACTTCTA ATGTTGACAA AAAGCAAGGC 
ATAAATGAAG ATCAAGAGGA GAGCCAGAAG CCAAGATTAG GTGAAGGGTG TGAACCAATA 
TCTAAACGAC AAATGAAAAA ACTAATAAAA CAGAAACAAT GGGAAGAGCA ACGGGAACTC 
CGCAAACAAA AGCGAAAAGA AAAACGCAAG AGGAAAAAAT TAGAGCGACA ATGTCAAATG 
GAACCAAACT CAGATGGACA TGACAGAAAA CGTGTTCGAA GAGATGTTGT TCATAGCACC 
CTTCGCCTTA TTATTGACTG TAGTTTTGAT CACTTGATGG TATTAAAGGA CATTAAGAAA 
CTTCATAAGC AGATTCAACG ATGTTACGCA GAAAACCGAC GGGCACTGCA TCCTGTGCAG 
TTTTACTTGA CAAGCCACGG AGGCCAGCTG AAAAAGAACA TGGATGAAAA TGACAAAGGA 
TGGGTCAACT GGAAGGATAT CCATATCAAA CCAGAGCACT ATAGTGAACT CATAAAGAAA 
GAAGACCTGA TTTACCTTAC GTCAGATTCA CCTAATATAC TGAAGGAATT AGATGAATCA 
AAGGCCTATG TGATTGGAGG ATTAGTAGAT CACAACCATC ACAAGGGACT CACATATAAA 
CAAGCGTCAG ATTATGGAAT CAATCATGCA CAGCTCCCAC TTGGAAATTT TGTGAAGATG 
AATAGTCGAA AAGTTTTGGC AGTTAATCAT GTGTTTGAAA TTATTCTGGA ATACCTGGAA 
ACAAGAGACT GGCAAGAAGC ATTTTTTACT ATCTTGCCCC AACGGAAAGG AGCTGTTCCC 
ACAGACAAAG CCTGTGAAAG TGCTTCTCAT GACAATCAGT CTGTCAGGAT GGAGGAAGGT 
GGATCGGACA GTGATTCCAG TGAGGAGGAA TATAGCAGAA ATGAACTAGA TTCACCACAT 
GAAGAAAAGC AGGATAAGGA AAATCACACT GAATCTACAG TGAACTCTCT GCCACACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24413
Clone ID Related Accession (Same CDS sequence) NM_001134665.2 , NM_001134665.3 , XM_005263352.3 , NM_152292.4 , XM_006714417.2 , NM_001375881.1 , NM_001134666.3 , NM_001375880.1 , NM_152292.5 , NM_001134666.2
Accession Version NM_152292.4 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1020bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-02-20
Organism Homo sapiens(human)
Product tRNA methyltransferase 10 homolog A
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC028373.2, AC083902.5 and AW242551.1. On Sep 4, 2008 this sequence version replaced NM_152292.3. Transcript Variant: This variant (1) represents the longest transcript. All three variants encode the same protein. ##Evidence-Data-START## Transcript exon combination :: BC028373.2, EU668342.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGTCATCTG AAATGTTGCC AGCATTTATT GAAACTTCTA ATGTTGACAA AAAGCAAGGC 
ATAAATGAAG ATCAAGAGGA GAGCCAGAAG CCAAGATTAG GTGAAGGGTG TGAACCAATA 
TCTAAACGAC AAATGAAAAA ACTAATAAAA CAGAAACAAT GGGAAGAGCA ACGGGAACTC 
CGCAAACAAA AGCGAAAAGA AAAACGCAAG AGGAAAAAAT TAGAGCGACA ATGTCAAATG 
GAACCAAACT CAGATGGACA TGACAGAAAA CGTGTTCGAA GAGATGTTGT TCATAGCACC 
CTTCGCCTTA TTATTGACTG TAGTTTTGAT CACTTGATGG TATTAAAGGA CATTAAGAAA 
CTTCATAAGC AGATTCAACG ATGTTACGCA GAAAACCGAC GGGCACTGCA TCCTGTGCAG 
TTTTACTTGA CAAGCCACGG AGGCCAGCTG AAAAAGAACA TGGATGAAAA TGACAAAGGA 
TGGGTCAACT GGAAGGATAT CCATATCAAA CCAGAGCACT ATAGTGAACT CATAAAGAAA 
GAAGACCTGA TTTACCTTAC GTCAGATTCA CCTAATATAC TGAAGGAATT AGATGAATCA 
AAGGCCTATG TGATTGGAGG ATTAGTAGAT CACAACCATC ACAAGGGACT CACATATAAA 
CAAGCGTCAG ATTATGGAAT CAATCATGCA CAGCTCCCAC TTGGAAATTT TGTGAAGATG 
AATAGTCGAA AAGTTTTGGC AGTTAATCAT GTGTTTGAAA TTATTCTGGA ATACCTGGAA 
ACAAGAGACT GGCAAGAAGC ATTTTTTACT ATCTTGCCCC AACGGAAAGG AGCTGTTCCC 
ACAGACAAAG CCTGTGAAAG TGCTTCTCAT GACAATCAGT CTGTCAGGAT GGAGGAAGGT 
GGATCGGACA GTGATTCCAG TGAGGAGGAA TATAGCAGAA ATGAACTAGA TTCACCACAT 
GAAGAAAAGC AGGATAAGGA AAATCACACT GAATCTACAG TGAACTCTCT GCCACACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_689505.1
CDS314..1333
Translation

Target ORF information:

RefSeq Version NM_152292.4
Organism Homo sapiens(human)
Definition Homo sapiens tRNA methyltransferase 10A (TRMT10A), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_152292.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGTCATCTG AAATGTTGCC AGCATTTATT GAAACTTCTA ATGTTGACAA AAAGCAAGGC 
ATAAATGAAG ATCAAGAGGA GAGCCAGAAG CCAAGATTAG GTGAAGGGTG TGAACCAATA 
TCTAAACGAC AAATGAAAAA ACTAATAAAA CAGAAACAAT GGGAAGAGCA ACGGGAACTC 
CGCAAACAAA AGCGAAAAGA AAAACGCAAG AGGAAAAAAT TAGAGCGACA ATGTCAAATG 
GAACCAAACT CAGATGGACA TGACAGAAAA CGTGTTCGAA GAGATGTTGT TCATAGCACC 
CTTCGCCTTA TTATTGACTG TAGTTTTGAT CACTTGATGG TATTAAAGGA CATTAAGAAA 
CTTCATAAGC AGATTCAACG ATGTTACGCA GAAAACCGAC GGGCACTGCA TCCTGTGCAG 
TTTTACTTGA CAAGCCACGG AGGCCAGCTG AAAAAGAACA TGGATGAAAA TGACAAAGGA 
TGGGTCAACT GGAAGGATAT CCATATCAAA CCAGAGCACT ATAGTGAACT CATAAAGAAA 
GAAGACCTGA TTTACCTTAC GTCAGATTCA CCTAATATAC TGAAGGAATT AGATGAATCA 
AAGGCCTATG TGATTGGAGG ATTAGTAGAT CACAACCATC ACAAGGGACT CACATATAAA 
CAAGCGTCAG ATTATGGAAT CAATCATGCA CAGCTCCCAC TTGGAAATTT TGTGAAGATG 
AATAGTCGAA AAGTTTTGGC AGTTAATCAT GTGTTTGAAA TTATTCTGGA ATACCTGGAA 
ACAAGAGACT GGCAAGAAGC ATTTTTTACT ATCTTGCCCC AACGGAAAGG AGCTGTTCCC 
ACAGACAAAG CCTGTGAAAG TGCTTCTCAT GACAATCAGT CTGTCAGGAT GGAGGAAGGT 
GGATCGGACA GTGATTCCAG TGAGGAGGAA TATAGCAGAA ATGAACTAGA TTCACCACAT 
GAAGAAAAGC AGGATAAGGA AAATCACACT GAATCTACAG TGAACTCTCT GCCACACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24413
Clone ID Related Accession (Same CDS sequence) NM_001134665.2 , NM_001134665.3 , XM_005263352.3 , NM_152292.4 , XM_006714417.2 , NM_001375881.1 , NM_001134666.3 , NM_001375880.1 , NM_152292.5 , NM_001134666.2
Accession Version NM_001375881.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1020bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-11-04
Organism Homo sapiens(human)
Product tRNA methyltransferase 10 homolog A isoform 1
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC083902.5. On Nov 5, 2019 this sequence version replaced XM_005263352.3. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.66653.1, SRR1660805.177553.1 [ECO:0000332] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGTCATCTG AAATGTTGCC AGCATTTATT GAAACTTCTA ATGTTGACAA AAAGCAAGGC 
ATAAATGAAG ATCAAGAGGA GAGCCAGAAG CCAAGATTAG GTGAAGGGTG TGAACCAATA 
TCTAAACGAC AAATGAAAAA ACTAATAAAA CAGAAACAAT GGGAAGAGCA ACGGGAACTC 
CGCAAACAAA AGCGAAAAGA AAAACGCAAG AGGAAAAAAT TAGAGCGACA ATGTCAAATG 
GAACCAAACT CAGATGGACA TGACAGAAAA CGTGTTCGAA GAGATGTTGT TCATAGCACC 
CTTCGCCTTA TTATTGACTG TAGTTTTGAT CACTTGATGG TATTAAAGGA CATTAAGAAA 
CTTCATAAGC AGATTCAACG ATGTTACGCA GAAAACCGAC GGGCACTGCA TCCTGTGCAG 
TTTTACTTGA CAAGCCACGG AGGCCAGCTG AAAAAGAACA TGGATGAAAA TGACAAAGGA 
TGGGTCAACT GGAAGGATAT CCATATCAAA CCAGAGCACT ATAGTGAACT CATAAAGAAA 
GAAGACCTGA TTTACCTTAC GTCAGATTCA CCTAATATAC TGAAGGAATT AGATGAATCA 
AAGGCCTATG TGATTGGAGG ATTAGTAGAT CACAACCATC ACAAGGGACT CACATATAAA 
CAAGCGTCAG ATTATGGAAT CAATCATGCA CAGCTCCCAC TTGGAAATTT TGTGAAGATG 
AATAGTCGAA AAGTTTTGGC AGTTAATCAT GTGTTTGAAA TTATTCTGGA ATACCTGGAA 
ACAAGAGACT GGCAAGAAGC ATTTTTTACT ATCTTGCCCC AACGGAAAGG AGCTGTTCCC 
ACAGACAAAG CCTGTGAAAG TGCTTCTCAT GACAATCAGT CTGTCAGGAT GGAGGAAGGT 
GGATCGGACA GTGATTCCAG TGAGGAGGAA TATAGCAGAA ATGAACTAGA TTCACCACAT 
GAAGAAAAGC AGGATAAGGA AAATCACACT GAATCTACAG TGAACTCTCT GCCACACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001362810.1
CDS179..1198
Translation

Target ORF information:

RefSeq Version NM_001375881.1
Organism Homo sapiens(human)
Definition Homo sapiens tRNA methyltransferase 10A (TRMT10A), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001375881.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGTCATCTG AAATGTTGCC AGCATTTATT GAAACTTCTA ATGTTGACAA AAAGCAAGGC 
ATAAATGAAG ATCAAGAGGA GAGCCAGAAG CCAAGATTAG GTGAAGGGTG TGAACCAATA 
TCTAAACGAC AAATGAAAAA ACTAATAAAA CAGAAACAAT GGGAAGAGCA ACGGGAACTC 
CGCAAACAAA AGCGAAAAGA AAAACGCAAG AGGAAAAAAT TAGAGCGACA ATGTCAAATG 
GAACCAAACT CAGATGGACA TGACAGAAAA CGTGTTCGAA GAGATGTTGT TCATAGCACC 
CTTCGCCTTA TTATTGACTG TAGTTTTGAT CACTTGATGG TATTAAAGGA CATTAAGAAA 
CTTCATAAGC AGATTCAACG ATGTTACGCA GAAAACCGAC GGGCACTGCA TCCTGTGCAG 
TTTTACTTGA CAAGCCACGG AGGCCAGCTG AAAAAGAACA TGGATGAAAA TGACAAAGGA 
TGGGTCAACT GGAAGGATAT CCATATCAAA CCAGAGCACT ATAGTGAACT CATAAAGAAA 
GAAGACCTGA TTTACCTTAC GTCAGATTCA CCTAATATAC TGAAGGAATT AGATGAATCA 
AAGGCCTATG TGATTGGAGG ATTAGTAGAT CACAACCATC ACAAGGGACT CACATATAAA 
CAAGCGTCAG ATTATGGAAT CAATCATGCA CAGCTCCCAC TTGGAAATTT TGTGAAGATG 
AATAGTCGAA AAGTTTTGGC AGTTAATCAT GTGTTTGAAA TTATTCTGGA ATACCTGGAA 
ACAAGAGACT GGCAAGAAGC ATTTTTTACT ATCTTGCCCC AACGGAAAGG AGCTGTTCCC 
ACAGACAAAG CCTGTGAAAG TGCTTCTCAT GACAATCAGT CTGTCAGGAT GGAGGAAGGT 
GGATCGGACA GTGATTCCAG TGAGGAGGAA TATAGCAGAA ATGAACTAGA TTCACCACAT 
GAAGAAAAGC AGGATAAGGA AAATCACACT GAATCTACAG TGAACTCTCT GCCACACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24413
Clone ID Related Accession (Same CDS sequence) NM_001134665.2 , NM_001134665.3 , XM_005263352.3 , NM_152292.4 , XM_006714417.2 , NM_001375881.1 , NM_001134666.3 , NM_001375880.1 , NM_152292.5 , NM_001134666.2
Accession Version NM_001134666.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1020bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-11-04
Organism Homo sapiens(human)
Product tRNA methyltransferase 10 homolog A isoform 1
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB455183.1, AK000332.1, BC028373.2, AC083902.5 and AW242551.1. On May 31, 2019 this sequence version replaced NM_001134666.2. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.189192.1, SRR7346977.2192192.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGTCATCTG AAATGTTGCC AGCATTTATT GAAACTTCTA ATGTTGACAA AAAGCAAGGC 
ATAAATGAAG ATCAAGAGGA GAGCCAGAAG CCAAGATTAG GTGAAGGGTG TGAACCAATA 
TCTAAACGAC AAATGAAAAA ACTAATAAAA CAGAAACAAT GGGAAGAGCA ACGGGAACTC 
CGCAAACAAA AGCGAAAAGA AAAACGCAAG AGGAAAAAAT TAGAGCGACA ATGTCAAATG 
GAACCAAACT CAGATGGACA TGACAGAAAA CGTGTTCGAA GAGATGTTGT TCATAGCACC 
CTTCGCCTTA TTATTGACTG TAGTTTTGAT CACTTGATGG TATTAAAGGA CATTAAGAAA 
CTTCATAAGC AGATTCAACG ATGTTACGCA GAAAACCGAC GGGCACTGCA TCCTGTGCAG 
TTTTACTTGA CAAGCCACGG AGGCCAGCTG AAAAAGAACA TGGATGAAAA TGACAAAGGA 
TGGGTCAACT GGAAGGATAT CCATATCAAA CCAGAGCACT ATAGTGAACT CATAAAGAAA 
GAAGACCTGA TTTACCTTAC GTCAGATTCA CCTAATATAC TGAAGGAATT AGATGAATCA 
AAGGCCTATG TGATTGGAGG ATTAGTAGAT CACAACCATC ACAAGGGACT CACATATAAA 
CAAGCGTCAG ATTATGGAAT CAATCATGCA CAGCTCCCAC TTGGAAATTT TGTGAAGATG 
AATAGTCGAA AAGTTTTGGC AGTTAATCAT GTGTTTGAAA TTATTCTGGA ATACCTGGAA 
ACAAGAGACT GGCAAGAAGC ATTTTTTACT ATCTTGCCCC AACGGAAAGG AGCTGTTCCC 
ACAGACAAAG CCTGTGAAAG TGCTTCTCAT GACAATCAGT CTGTCAGGAT GGAGGAAGGT 
GGATCGGACA GTGATTCCAG TGAGGAGGAA TATAGCAGAA ATGAACTAGA TTCACCACAT 
GAAGAAAAGC AGGATAAGGA AAATCACACT GAATCTACAG TGAACTCTCT GCCACACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001128138.1
CDS140..1159
Translation

Target ORF information:

RefSeq Version NM_001134666.3
Organism Homo sapiens(human)
Definition Homo sapiens tRNA methyltransferase 10A (TRMT10A), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001134666.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGTCATCTG AAATGTTGCC AGCATTTATT GAAACTTCTA ATGTTGACAA AAAGCAAGGC 
ATAAATGAAG ATCAAGAGGA GAGCCAGAAG CCAAGATTAG GTGAAGGGTG TGAACCAATA 
TCTAAACGAC AAATGAAAAA ACTAATAAAA CAGAAACAAT GGGAAGAGCA ACGGGAACTC 
CGCAAACAAA AGCGAAAAGA AAAACGCAAG AGGAAAAAAT TAGAGCGACA ATGTCAAATG 
GAACCAAACT CAGATGGACA TGACAGAAAA CGTGTTCGAA GAGATGTTGT TCATAGCACC 
CTTCGCCTTA TTATTGACTG TAGTTTTGAT CACTTGATGG TATTAAAGGA CATTAAGAAA 
CTTCATAAGC AGATTCAACG ATGTTACGCA GAAAACCGAC GGGCACTGCA TCCTGTGCAG 
TTTTACTTGA CAAGCCACGG AGGCCAGCTG AAAAAGAACA TGGATGAAAA TGACAAAGGA 
TGGGTCAACT GGAAGGATAT CCATATCAAA CCAGAGCACT ATAGTGAACT CATAAAGAAA 
GAAGACCTGA TTTACCTTAC GTCAGATTCA CCTAATATAC TGAAGGAATT AGATGAATCA 
AAGGCCTATG TGATTGGAGG ATTAGTAGAT CACAACCATC ACAAGGGACT CACATATAAA 
CAAGCGTCAG ATTATGGAAT CAATCATGCA CAGCTCCCAC TTGGAAATTT TGTGAAGATG 
AATAGTCGAA AAGTTTTGGC AGTTAATCAT GTGTTTGAAA TTATTCTGGA ATACCTGGAA 
ACAAGAGACT GGCAAGAAGC ATTTTTTACT ATCTTGCCCC AACGGAAAGG AGCTGTTCCC 
ACAGACAAAG CCTGTGAAAG TGCTTCTCAT GACAATCAGT CTGTCAGGAT GGAGGAAGGT 
GGATCGGACA GTGATTCCAG TGAGGAGGAA TATAGCAGAA ATGAACTAGA TTCACCACAT 
GAAGAAAAGC AGGATAAGGA AAATCACACT GAATCTACAG TGAACTCTCT GCCACACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24413
Clone ID Related Accession (Same CDS sequence) NM_001134665.2 , NM_001134665.3 , XM_005263352.3 , NM_152292.4 , XM_006714417.2 , NM_001375881.1 , NM_001134666.3 , NM_001375880.1 , NM_152292.5 , NM_001134666.2
Accession Version NM_001375880.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1020bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-11-04
Organism Homo sapiens(human)
Product tRNA methyltransferase 10 homolog A isoform 1
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC083902.5. On Nov 5, 2019 this sequence version replaced XM_006714417.2. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.1387031.1, SRR1660803.177086.1 [ECO:0000332] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGTCATCTG AAATGTTGCC AGCATTTATT GAAACTTCTA ATGTTGACAA AAAGCAAGGC 
ATAAATGAAG ATCAAGAGGA GAGCCAGAAG CCAAGATTAG GTGAAGGGTG TGAACCAATA 
TCTAAACGAC AAATGAAAAA ACTAATAAAA CAGAAACAAT GGGAAGAGCA ACGGGAACTC 
CGCAAACAAA AGCGAAAAGA AAAACGCAAG AGGAAAAAAT TAGAGCGACA ATGTCAAATG 
GAACCAAACT CAGATGGACA TGACAGAAAA CGTGTTCGAA GAGATGTTGT TCATAGCACC 
CTTCGCCTTA TTATTGACTG TAGTTTTGAT CACTTGATGG TATTAAAGGA CATTAAGAAA 
CTTCATAAGC AGATTCAACG ATGTTACGCA GAAAACCGAC GGGCACTGCA TCCTGTGCAG 
TTTTACTTGA CAAGCCACGG AGGCCAGCTG AAAAAGAACA TGGATGAAAA TGACAAAGGA 
TGGGTCAACT GGAAGGATAT CCATATCAAA CCAGAGCACT ATAGTGAACT CATAAAGAAA 
GAAGACCTGA TTTACCTTAC GTCAGATTCA CCTAATATAC TGAAGGAATT AGATGAATCA 
AAGGCCTATG TGATTGGAGG ATTAGTAGAT CACAACCATC ACAAGGGACT CACATATAAA 
CAAGCGTCAG ATTATGGAAT CAATCATGCA CAGCTCCCAC TTGGAAATTT TGTGAAGATG 
AATAGTCGAA AAGTTTTGGC AGTTAATCAT GTGTTTGAAA TTATTCTGGA ATACCTGGAA 
ACAAGAGACT GGCAAGAAGC ATTTTTTACT ATCTTGCCCC AACGGAAAGG AGCTGTTCCC 
ACAGACAAAG CCTGTGAAAG TGCTTCTCAT GACAATCAGT CTGTCAGGAT GGAGGAAGGT 
GGATCGGACA GTGATTCCAG TGAGGAGGAA TATAGCAGAA ATGAACTAGA TTCACCACAT 
GAAGAAAAGC AGGATAAGGA AAATCACACT GAATCTACAG TGAACTCTCT GCCACACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001362809.1
CDS190..1209
Translation

Target ORF information:

RefSeq Version NM_001375880.1
Organism Homo sapiens(human)
Definition Homo sapiens tRNA methyltransferase 10A (TRMT10A), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001375880.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGTCATCTG AAATGTTGCC AGCATTTATT GAAACTTCTA ATGTTGACAA AAAGCAAGGC 
ATAAATGAAG ATCAAGAGGA GAGCCAGAAG CCAAGATTAG GTGAAGGGTG TGAACCAATA 
TCTAAACGAC AAATGAAAAA ACTAATAAAA CAGAAACAAT GGGAAGAGCA ACGGGAACTC 
CGCAAACAAA AGCGAAAAGA AAAACGCAAG AGGAAAAAAT TAGAGCGACA ATGTCAAATG 
GAACCAAACT CAGATGGACA TGACAGAAAA CGTGTTCGAA GAGATGTTGT TCATAGCACC 
CTTCGCCTTA TTATTGACTG TAGTTTTGAT CACTTGATGG TATTAAAGGA CATTAAGAAA 
CTTCATAAGC AGATTCAACG ATGTTACGCA GAAAACCGAC GGGCACTGCA TCCTGTGCAG 
TTTTACTTGA CAAGCCACGG AGGCCAGCTG AAAAAGAACA TGGATGAAAA TGACAAAGGA 
TGGGTCAACT GGAAGGATAT CCATATCAAA CCAGAGCACT ATAGTGAACT CATAAAGAAA 
GAAGACCTGA TTTACCTTAC GTCAGATTCA CCTAATATAC TGAAGGAATT AGATGAATCA 
AAGGCCTATG TGATTGGAGG ATTAGTAGAT CACAACCATC ACAAGGGACT CACATATAAA 
CAAGCGTCAG ATTATGGAAT CAATCATGCA CAGCTCCCAC TTGGAAATTT TGTGAAGATG 
AATAGTCGAA AAGTTTTGGC AGTTAATCAT GTGTTTGAAA TTATTCTGGA ATACCTGGAA 
ACAAGAGACT GGCAAGAAGC ATTTTTTACT ATCTTGCCCC AACGGAAAGG AGCTGTTCCC 
ACAGACAAAG CCTGTGAAAG TGCTTCTCAT GACAATCAGT CTGTCAGGAT GGAGGAAGGT 
GGATCGGACA GTGATTCCAG TGAGGAGGAA TATAGCAGAA ATGAACTAGA TTCACCACAT 
GAAGAAAAGC AGGATAAGGA AAATCACACT GAATCTACAG TGAACTCTCT GCCACACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24413
Clone ID Related Accession (Same CDS sequence) NM_001134665.2 , NM_001134665.3 , XM_005263352.3 , NM_152292.4 , XM_006714417.2 , NM_001375881.1 , NM_001134666.3 , NM_001375880.1 , NM_152292.5 , NM_001134666.2
Accession Version NM_152292.5 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1020bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-11-04
Organism Homo sapiens(human)
Product tRNA methyltransferase 10 homolog A isoform 1
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC083902.5, BC028373.2 and AW242551.1. On May 31, 2019 this sequence version replaced NM_152292.4. ##Evidence-Data-START## Transcript exon combination :: EU668342.1, BC028373.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGTCATCTG AAATGTTGCC AGCATTTATT GAAACTTCTA ATGTTGACAA AAAGCAAGGC 
ATAAATGAAG ATCAAGAGGA GAGCCAGAAG CCAAGATTAG GTGAAGGGTG TGAACCAATA 
TCTAAACGAC AAATGAAAAA ACTAATAAAA CAGAAACAAT GGGAAGAGCA ACGGGAACTC 
CGCAAACAAA AGCGAAAAGA AAAACGCAAG AGGAAAAAAT TAGAGCGACA ATGTCAAATG 
GAACCAAACT CAGATGGACA TGACAGAAAA CGTGTTCGAA GAGATGTTGT TCATAGCACC 
CTTCGCCTTA TTATTGACTG TAGTTTTGAT CACTTGATGG TATTAAAGGA CATTAAGAAA 
CTTCATAAGC AGATTCAACG ATGTTACGCA GAAAACCGAC GGGCACTGCA TCCTGTGCAG 
TTTTACTTGA CAAGCCACGG AGGCCAGCTG AAAAAGAACA TGGATGAAAA TGACAAAGGA 
TGGGTCAACT GGAAGGATAT CCATATCAAA CCAGAGCACT ATAGTGAACT CATAAAGAAA 
GAAGACCTGA TTTACCTTAC GTCAGATTCA CCTAATATAC TGAAGGAATT AGATGAATCA 
AAGGCCTATG TGATTGGAGG ATTAGTAGAT CACAACCATC ACAAGGGACT CACATATAAA 
CAAGCGTCAG ATTATGGAAT CAATCATGCA CAGCTCCCAC TTGGAAATTT TGTGAAGATG 
AATAGTCGAA AAGTTTTGGC AGTTAATCAT GTGTTTGAAA TTATTCTGGA ATACCTGGAA 
ACAAGAGACT GGCAAGAAGC ATTTTTTACT ATCTTGCCCC AACGGAAAGG AGCTGTTCCC 
ACAGACAAAG CCTGTGAAAG TGCTTCTCAT GACAATCAGT CTGTCAGGAT GGAGGAAGGT 
GGATCGGACA GTGATTCCAG TGAGGAGGAA TATAGCAGAA ATGAACTAGA TTCACCACAT 
GAAGAAAAGC AGGATAAGGA AAATCACACT GAATCTACAG TGAACTCTCT GCCACACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_689505.1
CDS369..1388
Translation

Target ORF information:

RefSeq Version NM_152292.5
Organism Homo sapiens(human)
Definition Homo sapiens tRNA methyltransferase 10A (TRMT10A), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_152292.5

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGTCATCTG AAATGTTGCC AGCATTTATT GAAACTTCTA ATGTTGACAA AAAGCAAGGC 
ATAAATGAAG ATCAAGAGGA GAGCCAGAAG CCAAGATTAG GTGAAGGGTG TGAACCAATA 
TCTAAACGAC AAATGAAAAA ACTAATAAAA CAGAAACAAT GGGAAGAGCA ACGGGAACTC 
CGCAAACAAA AGCGAAAAGA AAAACGCAAG AGGAAAAAAT TAGAGCGACA ATGTCAAATG 
GAACCAAACT CAGATGGACA TGACAGAAAA CGTGTTCGAA GAGATGTTGT TCATAGCACC 
CTTCGCCTTA TTATTGACTG TAGTTTTGAT CACTTGATGG TATTAAAGGA CATTAAGAAA 
CTTCATAAGC AGATTCAACG ATGTTACGCA GAAAACCGAC GGGCACTGCA TCCTGTGCAG 
TTTTACTTGA CAAGCCACGG AGGCCAGCTG AAAAAGAACA TGGATGAAAA TGACAAAGGA 
TGGGTCAACT GGAAGGATAT CCATATCAAA CCAGAGCACT ATAGTGAACT CATAAAGAAA 
GAAGACCTGA TTTACCTTAC GTCAGATTCA CCTAATATAC TGAAGGAATT AGATGAATCA 
AAGGCCTATG TGATTGGAGG ATTAGTAGAT CACAACCATC ACAAGGGACT CACATATAAA 
CAAGCGTCAG ATTATGGAAT CAATCATGCA CAGCTCCCAC TTGGAAATTT TGTGAAGATG 
AATAGTCGAA AAGTTTTGGC AGTTAATCAT GTGTTTGAAA TTATTCTGGA ATACCTGGAA 
ACAAGAGACT GGCAAGAAGC ATTTTTTACT ATCTTGCCCC AACGGAAAGG AGCTGTTCCC 
ACAGACAAAG CCTGTGAAAG TGCTTCTCAT GACAATCAGT CTGTCAGGAT GGAGGAAGGT 
GGATCGGACA GTGATTCCAG TGAGGAGGAA TATAGCAGAA ATGAACTAGA TTCACCACAT 
GAAGAAAAGC AGGATAAGGA AAATCACACT GAATCTACAG TGAACTCTCT GCCACACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24413
Clone ID Related Accession (Same CDS sequence) NM_001134665.2 , NM_001134665.3 , XM_005263352.3 , NM_152292.4 , XM_006714417.2 , NM_001375881.1 , NM_001134666.3 , NM_001375880.1 , NM_152292.5 , NM_001134666.2
Accession Version NM_001134666.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1020bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-02-23
Organism Homo sapiens(human)
Product tRNA methyltransferase 10 homolog A
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB455183.1, AK000332.1, BC028373.2, AC083902.5 and AW242551.1. On Oct 17, 2014 this sequence version replaced NM_001134666.1. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. All three variants encode the same protein. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.189192.1, AK000332.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGTCATCTG AAATGTTGCC AGCATTTATT GAAACTTCTA ATGTTGACAA AAAGCAAGGC 
ATAAATGAAG ATCAAGAGGA GAGCCAGAAG CCAAGATTAG GTGAAGGGTG TGAACCAATA 
TCTAAACGAC AAATGAAAAA ACTAATAAAA CAGAAACAAT GGGAAGAGCA ACGGGAACTC 
CGCAAACAAA AGCGAAAAGA AAAACGCAAG AGGAAAAAAT TAGAGCGACA ATGTCAAATG 
GAACCAAACT CAGATGGACA TGACAGAAAA CGTGTTCGAA GAGATGTTGT TCATAGCACC 
CTTCGCCTTA TTATTGACTG TAGTTTTGAT CACTTGATGG TATTAAAGGA CATTAAGAAA 
CTTCATAAGC AGATTCAACG ATGTTACGCA GAAAACCGAC GGGCACTGCA TCCTGTGCAG 
TTTTACTTGA CAAGCCACGG AGGCCAGCTG AAAAAGAACA TGGATGAAAA TGACAAAGGA 
TGGGTCAACT GGAAGGATAT CCATATCAAA CCAGAGCACT ATAGTGAACT CATAAAGAAA 
GAAGACCTGA TTTACCTTAC GTCAGATTCA CCTAATATAC TGAAGGAATT AGATGAATCA 
AAGGCCTATG TGATTGGAGG ATTAGTAGAT CACAACCATC ACAAGGGACT CACATATAAA 
CAAGCGTCAG ATTATGGAAT CAATCATGCA CAGCTCCCAC TTGGAAATTT TGTGAAGATG 
AATAGTCGAA AAGTTTTGGC AGTTAATCAT GTGTTTGAAA TTATTCTGGA ATACCTGGAA 
ACAAGAGACT GGCAAGAAGC ATTTTTTACT ATCTTGCCCC AACGGAAAGG AGCTGTTCCC 
ACAGACAAAG CCTGTGAAAG TGCTTCTCAT GACAATCAGT CTGTCAGGAT GGAGGAAGGT 
GGATCGGACA GTGATTCCAG TGAGGAGGAA TATAGCAGAA ATGAACTAGA TTCACCACAT 
GAAGAAAAGC AGGATAAGGA AAATCACACT GAATCTACAG TGAACTCTCT GCCACACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001128138.1
CDS158..1177
Translation

Target ORF information:

RefSeq Version NM_001134666.2
Organism Homo sapiens(human)
Definition Homo sapiens tRNA methyltransferase 10A (TRMT10A), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001134666.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGTCATCTG AAATGTTGCC AGCATTTATT GAAACTTCTA ATGTTGACAA AAAGCAAGGC 
ATAAATGAAG ATCAAGAGGA GAGCCAGAAG CCAAGATTAG GTGAAGGGTG TGAACCAATA 
TCTAAACGAC AAATGAAAAA ACTAATAAAA CAGAAACAAT GGGAAGAGCA ACGGGAACTC 
CGCAAACAAA AGCGAAAAGA AAAACGCAAG AGGAAAAAAT TAGAGCGACA ATGTCAAATG 
GAACCAAACT CAGATGGACA TGACAGAAAA CGTGTTCGAA GAGATGTTGT TCATAGCACC 
CTTCGCCTTA TTATTGACTG TAGTTTTGAT CACTTGATGG TATTAAAGGA CATTAAGAAA 
CTTCATAAGC AGATTCAACG ATGTTACGCA GAAAACCGAC GGGCACTGCA TCCTGTGCAG 
TTTTACTTGA CAAGCCACGG AGGCCAGCTG AAAAAGAACA TGGATGAAAA TGACAAAGGA 
TGGGTCAACT GGAAGGATAT CCATATCAAA CCAGAGCACT ATAGTGAACT CATAAAGAAA 
GAAGACCTGA TTTACCTTAC GTCAGATTCA CCTAATATAC TGAAGGAATT AGATGAATCA 
AAGGCCTATG TGATTGGAGG ATTAGTAGAT CACAACCATC ACAAGGGACT CACATATAAA 
CAAGCGTCAG ATTATGGAAT CAATCATGCA CAGCTCCCAC TTGGAAATTT TGTGAAGATG 
AATAGTCGAA AAGTTTTGGC AGTTAATCAT GTGTTTGAAA TTATTCTGGA ATACCTGGAA 
ACAAGAGACT GGCAAGAAGC ATTTTTTACT ATCTTGCCCC AACGGAAAGG AGCTGTTCCC 
ACAGACAAAG CCTGTGAAAG TGCTTCTCAT GACAATCAGT CTGTCAGGAT GGAGGAAGGT 
GGATCGGACA GTGATTCCAG TGAGGAGGAA TATAGCAGAA ATGAACTAGA TTCACCACAT 
GAAGAAAAGC AGGATAAGGA AAATCACACT GAATCTACAG TGAACTCTCT GCCACACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24413
Clone ID Related Accession (Same CDS sequence) NM_001134665.2 , NM_001134665.3 , XM_005263352.3 , NM_152292.4 , XM_006714417.2 , NM_001375881.1 , NM_001134666.3 , NM_001375880.1 , NM_152292.5 , NM_001134666.2
Accession Version XM_005263352.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1020bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-06-12
Organism Homo sapiens(human)
Product tRNA methyltransferase 10 homolog A isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005263352.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGTCATCTG AAATGTTGCC AGCATTTATT GAAACTTCTA ATGTTGACAA AAAGCAAGGC 
ATAAATGAAG ATCAAGAGGA GAGCCAGAAG CCAAGATTAG GTGAAGGGTG TGAACCAATA 
TCTAAACGAC AAATGAAAAA ACTAATAAAA CAGAAACAAT GGGAAGAGCA ACGGGAACTC 
CGCAAACAAA AGCGAAAAGA AAAACGCAAG AGGAAAAAAT TAGAGCGACA ATGTCAAATG 
GAACCAAACT CAGATGGACA TGACAGAAAA CGTGTTCGAA GAGATGTTGT TCATAGCACC 
CTTCGCCTTA TTATTGACTG TAGTTTTGAT CACTTGATGG TATTAAAGGA CATTAAGAAA 
CTTCATAAGC AGATTCAACG ATGTTACGCA GAAAACCGAC GGGCACTGCA TCCTGTGCAG 
TTTTACTTGA CAAGCCACGG AGGCCAGCTG AAAAAGAACA TGGATGAAAA TGACAAAGGA 
TGGGTCAACT GGAAGGATAT CCATATCAAA CCAGAGCACT ATAGTGAACT CATAAAGAAA 
GAAGACCTGA TTTACCTTAC GTCAGATTCA CCTAATATAC TGAAGGAATT AGATGAATCA 
AAGGCCTATG TGATTGGAGG ATTAGTAGAT CACAACCATC ACAAGGGACT CACATATAAA 
CAAGCGTCAG ATTATGGAAT CAATCATGCA CAGCTCCCAC TTGGAAATTT TGTGAAGATG 
AATAGTCGAA AAGTTTTGGC AGTTAATCAT GTGTTTGAAA TTATTCTGGA ATACCTGGAA 
ACAAGAGACT GGCAAGAAGC ATTTTTTACT ATCTTGCCCC AACGGAAAGG AGCTGTTCCC 
ACAGACAAAG CCTGTGAAAG TGCTTCTCAT GACAATCAGT CTGTCAGGAT GGAGGAAGGT 
GGATCGGACA GTGATTCCAG TGAGGAGGAA TATAGCAGAA ATGAACTAGA TTCACCACAT 
GAAGAAAAGC AGGATAAGGA AAATCACACT GAATCTACAG TGAACTCTCT GCCACACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005263409.1
CDS148..1167
Translation

Target ORF information:

RefSeq Version XM_005263352.3
Organism Homo sapiens(human)
Definition Homo sapiens tRNA methyltransferase 10A (TRMT10A), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005263352.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGTCATCTG AAATGTTGCC AGCATTTATT GAAACTTCTA ATGTTGACAA AAAGCAAGGC 
ATAAATGAAG ATCAAGAGGA GAGCCAGAAG CCAAGATTAG GTGAAGGGTG TGAACCAATA 
TCTAAACGAC AAATGAAAAA ACTAATAAAA CAGAAACAAT GGGAAGAGCA ACGGGAACTC 
CGCAAACAAA AGCGAAAAGA AAAACGCAAG AGGAAAAAAT TAGAGCGACA ATGTCAAATG 
GAACCAAACT CAGATGGACA TGACAGAAAA CGTGTTCGAA GAGATGTTGT TCATAGCACC 
CTTCGCCTTA TTATTGACTG TAGTTTTGAT CACTTGATGG TATTAAAGGA CATTAAGAAA 
CTTCATAAGC AGATTCAACG ATGTTACGCA GAAAACCGAC GGGCACTGCA TCCTGTGCAG 
TTTTACTTGA CAAGCCACGG AGGCCAGCTG AAAAAGAACA TGGATGAAAA TGACAAAGGA 
TGGGTCAACT GGAAGGATAT CCATATCAAA CCAGAGCACT ATAGTGAACT CATAAAGAAA 
GAAGACCTGA TTTACCTTAC GTCAGATTCA CCTAATATAC TGAAGGAATT AGATGAATCA 
AAGGCCTATG TGATTGGAGG ATTAGTAGAT CACAACCATC ACAAGGGACT CACATATAAA 
CAAGCGTCAG ATTATGGAAT CAATCATGCA CAGCTCCCAC TTGGAAATTT TGTGAAGATG 
AATAGTCGAA AAGTTTTGGC AGTTAATCAT GTGTTTGAAA TTATTCTGGA ATACCTGGAA 
ACAAGAGACT GGCAAGAAGC ATTTTTTACT ATCTTGCCCC AACGGAAAGG AGCTGTTCCC 
ACAGACAAAG CCTGTGAAAG TGCTTCTCAT GACAATCAGT CTGTCAGGAT GGAGGAAGGT 
GGATCGGACA GTGATTCCAG TGAGGAGGAA TATAGCAGAA ATGAACTAGA TTCACCACAT 
GAAGAAAAGC AGGATAAGGA AAATCACACT GAATCTACAG TGAACTCTCT GCCACACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24413
Clone ID Related Accession (Same CDS sequence) NM_001134665.2 , NM_001134665.3 , XM_005263352.3 , NM_152292.4 , XM_006714417.2 , NM_001375881.1 , NM_001134666.3 , NM_001375880.1 , NM_152292.5 , NM_001134666.2
Accession Version XM_006714417.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1020bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-06-12
Organism Homo sapiens(human)
Product tRNA methyltransferase 10 homolog A isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006714417.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGTCATCTG AAATGTTGCC AGCATTTATT GAAACTTCTA ATGTTGACAA AAAGCAAGGC 
ATAAATGAAG ATCAAGAGGA GAGCCAGAAG CCAAGATTAG GTGAAGGGTG TGAACCAATA 
TCTAAACGAC AAATGAAAAA ACTAATAAAA CAGAAACAAT GGGAAGAGCA ACGGGAACTC 
CGCAAACAAA AGCGAAAAGA AAAACGCAAG AGGAAAAAAT TAGAGCGACA ATGTCAAATG 
GAACCAAACT CAGATGGACA TGACAGAAAA CGTGTTCGAA GAGATGTTGT TCATAGCACC 
CTTCGCCTTA TTATTGACTG TAGTTTTGAT CACTTGATGG TATTAAAGGA CATTAAGAAA 
CTTCATAAGC AGATTCAACG ATGTTACGCA GAAAACCGAC GGGCACTGCA TCCTGTGCAG 
TTTTACTTGA CAAGCCACGG AGGCCAGCTG AAAAAGAACA TGGATGAAAA TGACAAAGGA 
TGGGTCAACT GGAAGGATAT CCATATCAAA CCAGAGCACT ATAGTGAACT CATAAAGAAA 
GAAGACCTGA TTTACCTTAC GTCAGATTCA CCTAATATAC TGAAGGAATT AGATGAATCA 
AAGGCCTATG TGATTGGAGG ATTAGTAGAT CACAACCATC ACAAGGGACT CACATATAAA 
CAAGCGTCAG ATTATGGAAT CAATCATGCA CAGCTCCCAC TTGGAAATTT TGTGAAGATG 
AATAGTCGAA AAGTTTTGGC AGTTAATCAT GTGTTTGAAA TTATTCTGGA ATACCTGGAA 
ACAAGAGACT GGCAAGAAGC ATTTTTTACT ATCTTGCCCC AACGGAAAGG AGCTGTTCCC 
ACAGACAAAG CCTGTGAAAG TGCTTCTCAT GACAATCAGT CTGTCAGGAT GGAGGAAGGT 
GGATCGGACA GTGATTCCAG TGAGGAGGAA TATAGCAGAA ATGAACTAGA TTCACCACAT 
GAAGAAAAGC AGGATAAGGA AAATCACACT GAATCTACAG TGAACTCTCT GCCACACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006714480.1
CDS190..1209
Translation

Target ORF information:

RefSeq Version XM_006714417.2
Organism Homo sapiens(human)
Definition Homo sapiens tRNA methyltransferase 10A (TRMT10A), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006714417.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGTCATCTG AAATGTTGCC AGCATTTATT GAAACTTCTA ATGTTGACAA AAAGCAAGGC 
ATAAATGAAG ATCAAGAGGA GAGCCAGAAG CCAAGATTAG GTGAAGGGTG TGAACCAATA 
TCTAAACGAC AAATGAAAAA ACTAATAAAA CAGAAACAAT GGGAAGAGCA ACGGGAACTC 
CGCAAACAAA AGCGAAAAGA AAAACGCAAG AGGAAAAAAT TAGAGCGACA ATGTCAAATG 
GAACCAAACT CAGATGGACA TGACAGAAAA CGTGTTCGAA GAGATGTTGT TCATAGCACC 
CTTCGCCTTA TTATTGACTG TAGTTTTGAT CACTTGATGG TATTAAAGGA CATTAAGAAA 
CTTCATAAGC AGATTCAACG ATGTTACGCA GAAAACCGAC GGGCACTGCA TCCTGTGCAG 
TTTTACTTGA CAAGCCACGG AGGCCAGCTG AAAAAGAACA TGGATGAAAA TGACAAAGGA 
TGGGTCAACT GGAAGGATAT CCATATCAAA CCAGAGCACT ATAGTGAACT CATAAAGAAA 
GAAGACCTGA TTTACCTTAC GTCAGATTCA CCTAATATAC TGAAGGAATT AGATGAATCA 
AAGGCCTATG TGATTGGAGG ATTAGTAGAT CACAACCATC ACAAGGGACT CACATATAAA 
CAAGCGTCAG ATTATGGAAT CAATCATGCA CAGCTCCCAC TTGGAAATTT TGTGAAGATG 
AATAGTCGAA AAGTTTTGGC AGTTAATCAT GTGTTTGAAA TTATTCTGGA ATACCTGGAA 
ACAAGAGACT GGCAAGAAGC ATTTTTTACT ATCTTGCCCC AACGGAAAGG AGCTGTTCCC 
ACAGACAAAG CCTGTGAAAG TGCTTCTCAT GACAATCAGT CTGTCAGGAT GGAGGAAGGT 
GGATCGGACA GTGATTCCAG TGAGGAGGAA TATAGCAGAA ATGAACTAGA TTCACCACAT 
GAAGAAAAGC AGGATAAGGA AAATCACACT GAATCTACAG TGAACTCTCT GCCACACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu109021
Clone ID Related Accession (Same CDS sequence) NM_001375882.1
Accession Version NM_001375882.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 999bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-11-04
Organism Homo sapiens(human)
Product tRNA methyltransferase 10 homolog A isoform 2
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC083902.5. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.426435.1 [ECO:0000332] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGTCATCTG AAATGTTGCC AGCATTTATT GAAACTTCTA ATGTTGACAA AAAGCAAGGC 
ATAAATGAAG ATCAAGAGGA GAGCCAGAAG CCAAGATTAG GTGAAGGGTG TGAACCAATA 
TCTAAACGAC AAATGAAAAA ACTAATAAAA CAGAAACAAT GGGAAGAGCA ACGGGAACTC 
CGCAAACAAA AGCGAAAAGA AAAACGCAAG AGGAAAAAAT TAGAGCGACA ATGTCAAATG 
GAACCAAACT CAGATGGACA TGACAGAAAA CGTGTTCGAA GAGATGTTGT TCATAGCACC 
CTTCGCCTTA TTATTGACTG TAGTTTTGAT CACTTGATGG TATTAAAGGA CATTAAGAAA 
CTTCATAAGC AGATTCAACG ATGTTACGCA GAAAACCGAC GGGCACTGCA TCCTGTGCAG 
TTTTACTTGA CAAGCCACGG AGGCCAGCTG AAAAAGAACA TGGATGAAAA TGACAAAGGA 
TGGGTCAACT GGAAGGATAT CCATATCAAA CCAGAGCACT ATAGTGAACT CATAAAGAAA 
GAAGACCTGA TTTACCTTAC GTCAGATTCA CCTAATATAC TGAAGGAATT AGATGAATCA 
AAGGCCTATG TGATTGGAGG ATTAGGACTC ACATATAAAC AAGCGTCAGA TTATGGAATC 
AATCATGCAC AGCTCCCACT TGGAAATTTT GTGAAGATGA ATAGTCGAAA AGTTTTGGCA 
GTTAATCATG TGTTTGAAAT TATTCTGGAA TACCTGGAAA CAAGAGACTG GCAAGAAGCA 
TTTTTTACTA TCTTGCCCCA ACGGAAAGGA GCTGTTCCCA CAGACAAAGC CTGTGAAAGT 
GCTTCTCATG ACAATCAGTC TGTCAGGATG GAGGAAGGTG GATCGGACAG TGATTCCAGT 
GAGGAGGAAT ATAGCAGAAA TGAACTAGAT TCACCACATG AAGAAAAGCA GGATAAGGAA 
AATCACACTG AATCTACAGT GAACTCTCTG CCACACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001362811.1
CDS151..1149
Translation

Target ORF information:

RefSeq Version NM_001375882.1
Organism Homo sapiens(human)
Definition Homo sapiens tRNA methyltransferase 10A (TRMT10A), transcript variant 6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001375882.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGTCATCTG AAATGTTGCC AGCATTTATT GAAACTTCTA ATGTTGACAA AAAGCAAGGC 
ATAAATGAAG ATCAAGAGGA GAGCCAGAAG CCAAGATTAG GTGAAGGGTG TGAACCAATA 
TCTAAACGAC AAATGAAAAA ACTAATAAAA CAGAAACAAT GGGAAGAGCA ACGGGAACTC 
CGCAAACAAA AGCGAAAAGA AAAACGCAAG AGGAAAAAAT TAGAGCGACA ATGTCAAATG 
GAACCAAACT CAGATGGACA TGACAGAAAA CGTGTTCGAA GAGATGTTGT TCATAGCACC 
CTTCGCCTTA TTATTGACTG TAGTTTTGAT CACTTGATGG TATTAAAGGA CATTAAGAAA 
CTTCATAAGC AGATTCAACG ATGTTACGCA GAAAACCGAC GGGCACTGCA TCCTGTGCAG 
TTTTACTTGA CAAGCCACGG AGGCCAGCTG AAAAAGAACA TGGATGAAAA TGACAAAGGA 
TGGGTCAACT GGAAGGATAT CCATATCAAA CCAGAGCACT ATAGTGAACT CATAAAGAAA 
GAAGACCTGA TTTACCTTAC GTCAGATTCA CCTAATATAC TGAAGGAATT AGATGAATCA 
AAGGCCTATG TGATTGGAGG ATTAGGACTC ACATATAAAC AAGCGTCAGA TTATGGAATC 
AATCATGCAC AGCTCCCACT TGGAAATTTT GTGAAGATGA ATAGTCGAAA AGTTTTGGCA 
GTTAATCATG TGTTTGAAAT TATTCTGGAA TACCTGGAAA CAAGAGACTG GCAAGAAGCA 
TTTTTTACTA TCTTGCCCCA ACGGAAAGGA GCTGTTCCCA CAGACAAAGC CTGTGAAAGT 
GCTTCTCATG ACAATCAGTC TGTCAGGATG GAGGAAGGTG GATCGGACAG TGATTCCAGT 
GAGGAGGAAT ATAGCAGAAA TGAACTAGAT TCACCACATG AAGAAAAGCA GGATAAGGAA 
AATCACACTG AATCTACAGT GAACTCTCTG CCACACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

tRNA methyltransferase homologue gene TRMT10A mutation in young adult-onset diabetes with intellectual disability, microcephaly and epilepsy.
Diabetic medicine : a journal of the British Diabetic Association33(9)e21-5(2016 Sep)
Yew TW,McCreight L,Colclough K,Ellard S,Pearson ER


book

Homozygous deletion of TRMT10A as part of a contiguous gene deletion in a syndrome of failure to thrive, delayed puberty, intellectual disability and diabetes mellitus.
American journal of medical genetics. Part A167A(12)3167-73(2015 Dec)
Zung A,Kori M,Burundukov E,Ben-Yosef T,Tatoor Y,Granot E


book

TRMT10A dysfunction is associated with abnormalities in glucose homeostasis, short stature and microcephaly.
Journal of medical genetics51(9)581-6(2014 Sep)
Gillis D,Krishnamohan A,Yaacov B,Shaag A,Jackman JE,Elpeleg O


book

tRNA methyltransferase homolog gene TRMT10A mutation in young onset diabetes and primary microcephaly in humans.
PLoS genetics9(10)e1003888(2013 Oct)
Igoillo-Esteve M,Genin A,Lambert N,D?sir J,Pirson I,Abdulkarim B,Simonis N,Drielsma A,Marselli L,Marchetti P,Vanderhaeghen P,Eizirik DL,Wuyts W,Julier C,Chakera AJ,Ellard S,Hattersley AT,Abramowicz M,Cnop M