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CD86 cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol CD86
Entrez Gene ID 942
Full Name CD86 molecule
Synonyms B7-2,B7.2,B70,CD28LG2,LAB72
General protein information
Preferred Names

CD86 molecule

Names

T-lymphocyte activation antigen CD86
B-lymphocyte activation antigen B7-2
BU63
CD86 antigen (CD28 antigen ligand 2, B7-2 antigen)
CTLA-4 counter-receptor B7.2
FUN-1
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

3

3q13.33
Summary This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011].
Disorder MIM:

601020


Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001206924.1 NP_001193853.1 T-lymphocyte activation antigen CD86 isoform 4 precursor
NM_175862.4 NP_787058.4 T-lymphocyte activation antigen CD86 isoform 1 precursor
NM_176892.1 NP_795711.1 T-lymphocyte activation antigen CD86 isoform 3 precursor
NM_175862.5 NP_787058.5 T-lymphocyte activation antigen CD86 isoform 1 precursor
NM_006889.4 NP_008820.3 T-lymphocyte activation antigen CD86 isoform 2 precursor
NM_001206925.1 NP_001193854.1 T-lymphocyte activation antigen CD86 isoform 5

Pathways from BioSystems


Homologies


Homo sapiens (human) CD86 NP_787058.4
Bos taurus (cattle) CD86 NP_001033106.1
Pan troglodytes (chimpanzee) CD86 XP_526283.2
CD86 NP_001036109.1
Rattus norvegicus (Norway rat) Cd86 NP_064466.1
Gallus gallus (chicken) CD86 NP_001032928.1
Canis lupus familiaris (dog) CD86 NP_001003146.1
Mus musculus (house mouse) Cd86 NP_062261.3

Gene Ontology

The following CD86 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CD86 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu26653 NM_006889.4
Latest version!		 Homo sapiens CD86 molecule (CD86), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $154.50-$216.30
$309.00
OHu27158 NM_001206925.1
Latest version!		 Homo sapiens CD86 molecule (CD86), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $99.50-$139.30
$199.00
OHu26963 NM_001206924.1
Latest version!		 Homo sapiens CD86 molecule (CD86), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu27014 NM_176892.1
Latest version!		 Homo sapiens CD86 molecule (CD86), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu27348 NM_175862.4
Latest version!		 Homo sapiens CD86 molecule (CD86), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu107236 NM_175862.5
Latest version!		 Homo sapiens CD86 molecule (CD86), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $139.30
$199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu26653
Clone ID Related Accession (Same CDS sequence) NM_006889.4
Accession Version NM_006889.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 972bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-27
Organism Homo sapiens(human)
Product T-lymphocyte activation antigen CD86 isoform 2 precursor
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD705810.1, U04343.1, AK291497.1, BC040261.1 and BX093060.1. On May 11, 2011 this sequence version replaced NM_006889.3. Transcript Variant: This variant (2) includes an alternate exon in the 5' UTR, resulting in the use of a downstream start codon compared to variant 1. Isoform 2 has a shorter N-terminus than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U04343.1, SRR1163655.583722.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGGACTGA GTAACATTCT CTTTGTGATG GCCTTCCTGC TCTCTGGTGC TGCTCCTCTG 
AAGATTCAAG CTTATTTCAA TGAGACTGCA GACCTGCCAT GCCAATTTGC AAACTCTCAA 
AACCAAAGCC TGAGTGAGCT AGTAGTATTT TGGCAGGACC AGGAAAACTT GGTTCTGAAT 
GAGGTATACT TAGGCAAAGA GAAATTTGAC AGTGTTCATT CCAAGTATAT GGGCCGCACA 
AGTTTTGATT CGGACAGTTG GACCCTGAGA CTTCACAATC TTCAGATCAA GGACAAGGGC 
TTGTATCAAT GTATCATCCA TCACAAAAAG CCCACAGGAA TGATTCGCAT CCACCAGATG 
AATTCTGAAC TGTCAGTGCT TGCTAACTTC AGTCAACCTG AAATAGTACC AATTTCTAAT 
ATAACAGAAA ATGTGTACAT AAATTTGACC TGCTCATCTA TACACGGTTA CCCAGAACCT 
AAGAAGATGA GTGTTTTGCT AAGAACCAAG AATTCAACTA TCGAGTATGA TGGTATTATG 
CAGAAATCTC AAGATAATGT CACAGAACTG TACGACGTTT CCATCAGCTT GTCTGTTTCA 
TTCCCTGATG TTACGAGCAA TATGACCATC TTCTGTATTC TGGAAACTGA CAAGACGCGG 
CTTTTATCTT CACCTTTCTC TATAGAGCTT GAGGACCCTC AGCCTCCCCC AGACCACATT 
CCTTGGATTA CAGCTGTACT TCCAACAGTT ATTATATGTG TGATGGTTTT CTGTCTAATT 
CTATGGAAAT GGAAGAAGAA GAAGCGGCCT CGCAACTCTT ATAAATGTGG AACCAACACA 
ATGGAGAGGG AAGAGAGTGA ACAGACCAAG AAAAGAGAAA AAATCCATAT ACCTGAAAGA 
TCTGATGAAG CCCAGCGTGT TTTTAAAAGT TCGAAGACAT CTTCATGCGA CAAAAGTGAT 
ACATGTTTTT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_008820.3
CDS202..1173
Translation

Target ORF information:

RefSeq Version NM_006889.4
Organism Homo sapiens(human)
Definition Homo sapiens CD86 molecule (CD86), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_006889.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGGACTGA GTAACATTCT CTTTGTGATG GCCTTCCTGC TCTCTGGTGC TGCTCCTCTG 
AAGATTCAAG CTTATTTCAA TGAGACTGCA GACCTGCCAT GCCAATTTGC AAACTCTCAA 
AACCAAAGCC TGAGTGAGCT AGTAGTATTT TGGCAGGACC AGGAAAACTT GGTTCTGAAT 
GAGGTATACT TAGGCAAAGA GAAATTTGAC AGTGTTCATT CCAAGTATAT GGGCCGCACA 
AGTTTTGATT CGGACAGTTG GACCCTGAGA CTTCACAATC TTCAGATCAA GGACAAGGGC 
TTGTATCAAT GTATCATCCA TCACAAAAAG CCCACAGGAA TGATTCGCAT CCACCAGATG 
AATTCTGAAC TGTCAGTGCT TGCTAACTTC AGTCAACCTG AAATAGTACC AATTTCTAAT 
ATAACAGAAA ATGTGTACAT AAATTTGACC TGCTCATCTA TACACGGTTA CCCAGAACCT 
AAGAAGATGA GTGTTTTGCT AAGAACCAAG AATTCAACTA TCGAGTATGA TGGTATTATG 
CAGAAATCTC AAGATAATGT CACAGAACTG TACGACGTTT CCATCAGCTT GTCTGTTTCA 
TTCCCTGATG TTACGAGCAA TATGACCATC TTCTGTATTC TGGAAACTGA CAAGACGCGG 
CTTTTATCTT CACCTTTCTC TATAGAGCTT GAGGACCCTC AGCCTCCCCC AGACCACATT 
CCTTGGATTA CAGCTGTACT TCCAACAGTT ATTATATGTG TGATGGTTTT CTGTCTAATT 
CTATGGAAAT GGAAGAAGAA GAAGCGGCCT CGCAACTCTT ATAAATGTGG AACCAACACA 
ATGGAGAGGG AAGAGAGTGA ACAGACCAAG AAAAGAGAAA AAATCCATAT ACCTGAAAGA 
TCTGATGAAG CCCAGCGTGT TTTTAAAAGT TCGAAGACAT CTTCATGCGA CAAAAGTGAT 
ACATGTTTTT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27158
Clone ID Related Accession (Same CDS sequence) NM_001206925.1
Accession Version NM_001206925.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 744bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-27
Organism Homo sapiens(human)
Product T-lymphocyte activation antigen CD86 isoform 5
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA619360.1, AK316203.1, AK291497.1, BC040261.1 and BX093060.1. Transcript Variant: This variant (5) lacks an alternate coding exon compared to variant 1, that causes a frameshift. The resulting isoform (5) is made from a downstream translation start site compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.276932.1, AK316203.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGGCCGCA CAAGTTTTGA TTCGGACAGT TGGACCCTGA GACTTCACAA TCTTCAGATC 
AAGGACAAGG GCTTGTATCA ATGTATCATC CATCACAAAA AGCCCACAGG AATGATTCGC 
ATCCACCAGA TGAATTCTGA ACTGTCAGTG CTTGCTAACT TCAGTCAACC TGAAATAGTA 
CCAATTTCTA ATATAACAGA AAATGTGTAC ATAAATTTGA CCTGCTCATC TATACACGGT 
TACCCAGAAC CTAAGAAGAT GAGTGTTTTG CTAAGAACCA AGAATTCAAC TATCGAGTAT 
GATGGTATTA TGCAGAAATC TCAAGATAAT GTCACAGAAC TGTACGACGT TTCCATCAGC 
TTGTCTGTTT CATTCCCTGA TGTTACGAGC AATATGACCA TCTTCTGTAT TCTGGAAACT 
GACAAGACGC GGCTTTTATC TTCACCTTTC TCTATAGAGC TTGAGGACCC TCAGCCTCCC 
CCAGACCACA TTCCTTGGAT TACAGCTGTA CTTCCAACAG TTATTATATG TGTGATGGTT 
TTCTGTCTAA TTCTATGGAA ATGGAAGAAG AAGAAGCGGC CTCGCAACTC TTATAAATGT 
GGAACCAACA CAATGGAGAG GGAAGAGAGT GAACAGACCA AGAAAAGAGA AAAAATCCAT 
ATACCTGAAA GATCTGATGA AGCCCAGCGT GTTTTTAAAA GTTCGAAGAC ATCTTCATGC 
GACAAAAGTG ATACATGTTT TTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001193854.1
CDS325..1068
Translation

Target ORF information:

RefSeq Version NM_001206925.1
Organism Homo sapiens(human)
Definition Homo sapiens CD86 molecule (CD86), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001206925.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGGCCGCA CAAGTTTTGA TTCGGACAGT TGGACCCTGA GACTTCACAA TCTTCAGATC 
AAGGACAAGG GCTTGTATCA ATGTATCATC CATCACAAAA AGCCCACAGG AATGATTCGC 
ATCCACCAGA TGAATTCTGA ACTGTCAGTG CTTGCTAACT TCAGTCAACC TGAAATAGTA 
CCAATTTCTA ATATAACAGA AAATGTGTAC ATAAATTTGA CCTGCTCATC TATACACGGT 
TACCCAGAAC CTAAGAAGAT GAGTGTTTTG CTAAGAACCA AGAATTCAAC TATCGAGTAT 
GATGGTATTA TGCAGAAATC TCAAGATAAT GTCACAGAAC TGTACGACGT TTCCATCAGC 
TTGTCTGTTT CATTCCCTGA TGTTACGAGC AATATGACCA TCTTCTGTAT TCTGGAAACT 
GACAAGACGC GGCTTTTATC TTCACCTTTC TCTATAGAGC TTGAGGACCC TCAGCCTCCC 
CCAGACCACA TTCCTTGGAT TACAGCTGTA CTTCCAACAG TTATTATATG TGTGATGGTT 
TTCTGTCTAA TTCTATGGAA ATGGAAGAAG AAGAAGCGGC CTCGCAACTC TTATAAATGT 
GGAACCAACA CAATGGAGAG GGAAGAGAGT GAACAGACCA AGAAAAGAGA AAAAATCCAT 
ATACCTGAAA GATCTGATGA AGCCCAGCGT GTTTTTAAAA GTTCGAAGAC ATCTTCATGC 
GACAAAAGTG ATACATGTTT TTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26963
Clone ID Related Accession (Same CDS sequence) NM_001206924.1
Accession Version NM_001206924.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 654bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-27
Organism Homo sapiens(human)
Product T-lymphocyte activation antigen CD86 isoform 4 precursor
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA619360.1, AK294663.1, AK291497.1, BC040261.1 and BX093060.1. Transcript Variant: This variant (4) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (4) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK294663.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2146236, SAMEA2147920 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
ATGGATCCCC AGTGCACTAT GGGACTGAGT AACATTCTCT TTGTGATGGC CTTCCTGCTC 
TCTGCTAACT TCAGTCAACC TGAAATAGTA CCAATTTCTA ATATAACAGA AAATGTGTAC 
ATAAATTTGA CCTGCTCATC TATACACGGT TACCCAGAAC CTAAGAAGAT GAGTGTTTTG 
CTAAGAACCA AGAATTCAAC TATCGAGTAT GATGGTATTA TGCAGAAATC TCAAGATAAT 
GTCACAGAAC TGTACGACGT TTCCATCAGC TTGTCTGTTT CATTCCCTGA TGTTACGAGC 
AATATGACCA TCTTCTGTAT TCTGGAAACT GACAAGACGC GGCTTTTATC TTCACCTTTC 
TCTATAGAGC TTGAGGACCC TCAGCCTCCC CCAGACCACA TTCCTTGGAT TACAGCTGTA 
CTTCCAACAG TTATTATATG TGTGATGGTT TTCTGTCTAA TTCTATGGAA ATGGAAGAAG 
AAGAAGCGGC CTCGCAACTC TTATAAATGT GGAACCAACA CAATGGAGAG GGAAGAGAGT 
GAACAGACCA AGAAAAGAGA AAAAATCCAT ATACCTGAAA GATCTGATGA AGCCCAGCGT 
GTTTTTAAAA GTTCGAAGAC ATCTTCATGC GACAAAAGTG ATACATGTTT TTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001193853.1
CDS129..782
Translation

Target ORF information:

RefSeq Version NM_001206924.1
Organism Homo sapiens(human)
Definition Homo sapiens CD86 molecule (CD86), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001206924.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGATCCCC AGTGCACTAT GGGACTGAGT AACATTCTCT TTGTGATGGC CTTCCTGCTC 
TCTGCTAACT TCAGTCAACC TGAAATAGTA CCAATTTCTA ATATAACAGA AAATGTGTAC 
ATAAATTTGA CCTGCTCATC TATACACGGT TACCCAGAAC CTAAGAAGAT GAGTGTTTTG 
CTAAGAACCA AGAATTCAAC TATCGAGTAT GATGGTATTA TGCAGAAATC TCAAGATAAT 
GTCACAGAAC TGTACGACGT TTCCATCAGC TTGTCTGTTT CATTCCCTGA TGTTACGAGC 
AATATGACCA TCTTCTGTAT TCTGGAAACT GACAAGACGC GGCTTTTATC TTCACCTTTC 
TCTATAGAGC TTGAGGACCC TCAGCCTCCC CCAGACCACA TTCCTTGGAT TACAGCTGTA 
CTTCCAACAG TTATTATATG TGTGATGGTT TTCTGTCTAA TTCTATGGAA ATGGAAGAAG 
AAGAAGCGGC CTCGCAACTC TTATAAATGT GGAACCAACA CAATGGAGAG GGAAGAGAGT 
GAACAGACCA AGAAAAGAGA AAAAATCCAT ATACCTGAAA GATCTGATGA AGCCCAGCGT 
GTTTTTAAAA GTTCGAAGAC ATCTTCATGC GACAAAAGTG ATACATGTTT TTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27014
Clone ID Related Accession (Same CDS sequence) NM_176892.1
Accession Version NM_176892.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 828bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-27
Organism Homo sapiens(human)
Product T-lymphocyte activation antigen CD86 isoform 3 precursor
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD705810.1, U04343.1, AK291497.1, BC040261.1 and BX093060.1. Transcript Variant: This variant (3) includes an alternate exon in the 5' UTR, resulting in the use of a downstream start codon compared to variant 1. It also lacks an in-frame exon compared to variant 1. The resulting isoform (3, also known as CD86deltaTM) has a shorter N-terminus and lacks an internal segment compared to isoform 1. This isoform is the soluble form of this protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: PMID: 11021528, 11986949 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGGACTGA GTAACATTCT CTTTGTGATG GCCTTCCTGC TCTCTGGTGC TGCTCCTCTG 
AAGATTCAAG CTTATTTCAA TGAGACTGCA GACCTGCCAT GCCAATTTGC AAACTCTCAA 
AACCAAAGCC TGAGTGAGCT AGTAGTATTT TGGCAGGACC AGGAAAACTT GGTTCTGAAT 
GAGGTATACT TAGGCAAAGA GAAATTTGAC AGTGTTCATT CCAAGTATAT GGGCCGCACA 
AGTTTTGATT CGGACAGTTG GACCCTGAGA CTTCACAATC TTCAGATCAA GGACAAGGGC 
TTGTATCAAT GTATCATCCA TCACAAAAAG CCCACAGGAA TGATTCGCAT CCACCAGATG 
AATTCTGAAC TGTCAGTGCT TGCTAACTTC AGTCAACCTG AAATAGTACC AATTTCTAAT 
ATAACAGAAA ATGTGTACAT AAATTTGACC TGCTCATCTA TACACGGTTA CCCAGAACCT 
AAGAAGATGA GTGTTTTGCT AAGAACCAAG AATTCAACTA TCGAGTATGA TGGTATTATG 
CAGAAATCTC AAGATAATGT CACAGAACTG TACGACGTTT CCATCAGCTT GTCTGTTTCA 
TTCCCTGATG TTACGAGCAA TATGACCATC TTCTGTATTC TGGAAACTGA CAAGACGCGG 
CTTTTATCTT CACCTTTCTC TATAGGAACC AACACAATGG AGAGGGAAGA GAGTGAACAG 
ACCAAGAAAA GAGAAAAAAT CCATATACCT GAAAGATCTG ATGAAGCCCA GCGTGTTTTT 
AAAAGTTCGA AGACATCTTC ATGCGACAAA AGTGATACAT GTTTTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_795711.1
CDS202..1029
Translation

Target ORF information:

RefSeq Version NM_176892.1
Organism Homo sapiens(human)
Definition Homo sapiens CD86 molecule (CD86), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_176892.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGGACTGA GTAACATTCT CTTTGTGATG GCCTTCCTGC TCTCTGGTGC TGCTCCTCTG 
AAGATTCAAG CTTATTTCAA TGAGACTGCA GACCTGCCAT GCCAATTTGC AAACTCTCAA 
AACCAAAGCC TGAGTGAGCT AGTAGTATTT TGGCAGGACC AGGAAAACTT GGTTCTGAAT 
GAGGTATACT TAGGCAAAGA GAAATTTGAC AGTGTTCATT CCAAGTATAT GGGCCGCACA 
AGTTTTGATT CGGACAGTTG GACCCTGAGA CTTCACAATC TTCAGATCAA GGACAAGGGC 
TTGTATCAAT GTATCATCCA TCACAAAAAG CCCACAGGAA TGATTCGCAT CCACCAGATG 
AATTCTGAAC TGTCAGTGCT TGCTAACTTC AGTCAACCTG AAATAGTACC AATTTCTAAT 
ATAACAGAAA ATGTGTACAT AAATTTGACC TGCTCATCTA TACACGGTTA CCCAGAACCT 
AAGAAGATGA GTGTTTTGCT AAGAACCAAG AATTCAACTA TCGAGTATGA TGGTATTATG 
CAGAAATCTC AAGATAATGT CACAGAACTG TACGACGTTT CCATCAGCTT GTCTGTTTCA 
TTCCCTGATG TTACGAGCAA TATGACCATC TTCTGTATTC TGGAAACTGA CAAGACGCGG 
CTTTTATCTT CACCTTTCTC TATAGGAACC AACACAATGG AGAGGGAAGA GAGTGAACAG 
ACCAAGAAAA GAGAAAAAAT CCATATACCT GAAAGATCTG ATGAAGCCCA GCGTGTTTTT 
AAAAGTTCGA AGACATCTTC ATGCGACAAA AGTGATACAT GTTTTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27348
Clone ID Related Accession (Same CDS sequence) NM_175862.4
Accession Version NM_175862.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 990bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-12-05
Organism Homo sapiens(human)
Product T-lymphocyte activation antigen CD86 isoform 1 precursor
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA619360.1, BC040261.1, AK291497.1 and BX093060.1. This sequence is a reference standard in the RefSeqGene project. On May 11, 2011 this sequence version replaced NM_175862.3. Transcript Variant: This variant (1) encodes the longest isoform (1) of this protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.19992.1, SRR1803616.135317.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGATCCCC AGTGCACTAT GGGACTGAGT AACATTCTCT TTGTGATGGC CTTCCTGCTC 
TCTGGTGCTG CTCCTCTGAA GATTCAAGCT TATTTCAATG AGACTGCAGA CCTGCCATGC 
CAATTTGCAA ACTCTCAAAA CCAAAGCCTG AGTGAGCTAG TAGTATTTTG GCAGGACCAG 
GAAAACTTGG TTCTGAATGA GGTATACTTA GGCAAAGAGA AATTTGACAG TGTTCATTCC 
AAGTATATGG GCCGCACAAG TTTTGATTCG GACAGTTGGA CCCTGAGACT TCACAATCTT 
CAGATCAAGG ACAAGGGCTT GTATCAATGT ATCATCCATC ACAAAAAGCC CACAGGAATG 
ATTCGCATCC ACCAGATGAA TTCTGAACTG TCAGTGCTTG CTAACTTCAG TCAACCTGAA 
ATAGTACCAA TTTCTAATAT AACAGAAAAT GTGTACATAA ATTTGACCTG CTCATCTATA 
CACGGTTACC CAGAACCTAA GAAGATGAGT GTTTTGCTAA GAACCAAGAA TTCAACTATC 
GAGTATGATG GTATTATGCA GAAATCTCAA GATAATGTCA CAGAACTGTA CGACGTTTCC 
ATCAGCTTGT CTGTTTCATT CCCTGATGTT ACGAGCAATA TGACCATCTT CTGTATTCTG 
GAAACTGACA AGACGCGGCT TTTATCTTCA CCTTTCTCTA TAGAGCTTGA GGACCCTCAG 
CCTCCCCCAG ACCACATTCC TTGGATTACA GCTGTACTTC CAACAGTTAT TATATGTGTG 
ATGGTTTTCT GTCTAATTCT ATGGAAATGG AAGAAGAAGA AGCGGCCTCG CAACTCTTAT 
AAATGTGGAA CCAACACAAT GGAGAGGGAA GAGAGTGAAC AGACCAAGAA AAGAGAAAAA 
ATCCATATAC CTGAAAGATC TGATGAAGCC CAGCGTGTTT TTAAAAGTTC GAAGACATCT 
TCATGCGACA AAAGTGATAC ATGTTTTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_787058.4
CDS129..1118
Translation

Target ORF information:

RefSeq Version NM_175862.4
Organism Homo sapiens(human)
Definition Homo sapiens CD86 molecule (CD86), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_175862.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGATCCCC AGTGCACTAT GGGACTGAGT AACATTCTCT TTGTGATGGC CTTCCTGCTC 
TCTGGTGCTG CTCCTCTGAA GATTCAAGCT TATTTCAATG AGACTGCAGA CCTGCCATGC 
CAATTTGCAA ACTCTCAAAA CCAAAGCCTG AGTGAGCTAG TAGTATTTTG GCAGGACCAG 
GAAAACTTGG TTCTGAATGA GGTATACTTA GGCAAAGAGA AATTTGACAG TGTTCATTCC 
AAGTATATGG GCCGCACAAG TTTTGATTCG GACAGTTGGA CCCTGAGACT TCACAATCTT 
CAGATCAAGG ACAAGGGCTT GTATCAATGT ATCATCCATC ACAAAAAGCC CACAGGAATG 
ATTCGCATCC ACCAGATGAA TTCTGAACTG TCAGTGCTTG CTAACTTCAG TCAACCTGAA 
ATAGTACCAA TTTCTAATAT AACAGAAAAT GTGTACATAA ATTTGACCTG CTCATCTATA 
CACGGTTACC CAGAACCTAA GAAGATGAGT GTTTTGCTAA GAACCAAGAA TTCAACTATC 
GAGTATGATG GTATTATGCA GAAATCTCAA GATAATGTCA CAGAACTGTA CGACGTTTCC 
ATCAGCTTGT CTGTTTCATT CCCTGATGTT ACGAGCAATA TGACCATCTT CTGTATTCTG 
GAAACTGACA AGACGCGGCT TTTATCTTCA CCTTTCTCTA TAGAGCTTGA GGACCCTCAG 
CCTCCCCCAG ACCACATTCC TTGGATTACA GCTGTACTTC CAACAGTTAT TATATGTGTG 
ATGGTTTTCT GTCTAATTCT ATGGAAATGG AAGAAGAAGA AGCGGCCTCG CAACTCTTAT 
AAATGTGGAA CCAACACAAT GGAGAGGGAA GAGAGTGAAC AGACCAAGAA AAGAGAAAAA 
ATCCATATAC CTGAAAGATC TGATGAAGCC CAGCGTGTTT TTAAAAGTTC GAAGACATCT 
TCATGCGACA AAAGTGATAC ATGTTTTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu107236
Clone ID Related Accession (Same CDS sequence) NM_175862.5
Accession Version NM_175862.5 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 990bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-11-10
Organism Homo sapiens(human)
Product T-lymphocyte activation antigen CD86 isoform 1 precursor
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC068630.21. On Nov 23, 2018 this sequence version replaced NM_175862.4. Transcript Variant: This variant (1) encodes the longest isoform (1) of this protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC040261.1, SRR1803616.135317.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000330540.7/ ENSP00000332049.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGATCCCC AGTGCACTAT GGGACTGAGT AACATTCTCT TTGTGATGGC CTTCCTGCTC 
TCTGGTGCTG CTCCTCTGAA GATTCAAGCT TATTTCAATG AGACTGCAGA CCTGCCATGC 
CAATTTGCAA ACTCTCAAAA CCAAAGCCTG AGTGAGCTAG TAGTATTTTG GCAGGACCAG 
GAAAACTTGG TTCTGAATGA GGTATACTTA GGCAAAGAGA AATTTGACAG TGTTCATTCC 
AAGTATATGG GCCGCACAAG TTTTGATTCG GACAGTTGGA CCCTGAGACT TCACAATCTT 
CAGATCAAGG ACAAGGGCTT GTATCAATGT ATCATCCATC ACAAAAAGCC CACAGGAATG 
ATTCGCATCC ACCAGATGAA TTCTGAACTG TCAGTGCTTG CTAACTTCAG TCAACCTGAA 
ATAGTACCAA TTTCTAATAT AACAGAAAAT GTGTACATAA ATTTGACCTG CTCATCTATA 
CACGGTTACC CAGAACCTAA GAAGATGAGT GTTTTGCTAA GAACCAAGAA TTCAACTATC 
GAGTATGATG GTGTTATGCA GAAATCTCAA GATAATGTCA CAGAACTGTA CGACGTTTCC 
ATCAGCTTGT CTGTTTCATT CCCTGATGTT ACGAGCAATA TGACCATCTT CTGTATTCTG 
GAAACTGACA AGACGCGGCT TTTATCTTCA CCTTTCTCTA TAGAGCTTGA GGACCCTCAG 
CCTCCCCCAG ACCACATTCC TTGGATTACA GCTGTACTTC CAACAGTTAT TATATGTGTG 
ATGGTTTTCT GTCTAATTCT ATGGAAATGG AAGAAGAAGA AGCGGCCTCG CAACTCTTAT 
AAATGTGGAA CCAACACAAT GGAGAGGGAA GAGAGTGAAC AGACCAAGAA AAGAGAAAAA 
ATCCATATAC CTGAAAGATC TGATGAAGCC CAGCGTGTTT TTAAAAGTTC GAAGACATCT 
TCATGCGACA AAAGTGATAC ATGTTTTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_787058.5
CDS129..1118
Translation

Target ORF information:

RefSeq Version NM_175862.5
Organism Homo sapiens(human)
Definition Homo sapiens CD86 molecule (CD86), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_175862.5

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGATCCCC AGTGCACTAT GGGACTGAGT AACATTCTCT TTGTGATGGC CTTCCTGCTC 
TCTGGTGCTG CTCCTCTGAA GATTCAAGCT TATTTCAATG AGACTGCAGA CCTGCCATGC 
CAATTTGCAA ACTCTCAAAA CCAAAGCCTG AGTGAGCTAG TAGTATTTTG GCAGGACCAG 
GAAAACTTGG TTCTGAATGA GGTATACTTA GGCAAAGAGA AATTTGACAG TGTTCATTCC 
AAGTATATGG GCCGCACAAG TTTTGATTCG GACAGTTGGA CCCTGAGACT TCACAATCTT 
CAGATCAAGG ACAAGGGCTT GTATCAATGT ATCATCCATC ACAAAAAGCC CACAGGAATG 
ATTCGCATCC ACCAGATGAA TTCTGAACTG TCAGTGCTTG CTAACTTCAG TCAACCTGAA 
ATAGTACCAA TTTCTAATAT AACAGAAAAT GTGTACATAA ATTTGACCTG CTCATCTATA 
CACGGTTACC CAGAACCTAA GAAGATGAGT GTTTTGCTAA GAACCAAGAA TTCAACTATC 
GAGTATGATG GTGTTATGCA GAAATCTCAA GATAATGTCA CAGAACTGTA CGACGTTTCC 
ATCAGCTTGT CTGTTTCATT CCCTGATGTT ACGAGCAATA TGACCATCTT CTGTATTCTG 
GAAACTGACA AGACGCGGCT TTTATCTTCA CCTTTCTCTA TAGAGCTTGA GGACCCTCAG 
CCTCCCCCAG ACCACATTCC TTGGATTACA GCTGTACTTC CAACAGTTAT TATATGTGTG 
ATGGTTTTCT GTCTAATTCT ATGGAAATGG AAGAAGAAGA AGCGGCCTCG CAACTCTTAT 
AAATGTGGAA CCAACACAAT GGAGAGGGAA GAGAGTGAAC AGACCAAGAA AAGAGAAAAA 
ATCCATATAC CTGAAAGATC TGATGAAGCC CAGCGTGTTT TTAAAAGTTC GAAGACATCT 
TCATGCGACA AAAGTGATAC ATGTTTTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed