ORF cDNA Clones and Custom Clones » Species Summary » Pan troglodytes » SPTAN1 cDNA ORF clone

SPTAN1 cDNA ORF clone, Pan troglodytes(chimpanzee)

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Summary

Gene Symbol	SPTAN1
Entrez Gene ID	464771
Full Name	spectrin alpha, non-erythrocytic 1
General protein information	Preferred Names spectrin alpha, non-erythrocytic 1 Names spectrin alpha chain, non-erythrocytic 1 spectrin, alpha, non-erythrocytic 1 (alpha-fodrin)
Gene Type	protein-coding
Organism	Pan troglodytes(chimpanzee)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA	Protein	Name
XM_016961795.1	XP_016817284.1	spectrin alpha chain, non-erythrocytic 1 isoform X3
XM_016961795.2	XP_016817284.2	spectrin alpha chain, non-erythrocytic 1 isoform X3
XM_024345907.1	XP_024201675.1	spectrin alpha chain, non-erythrocytic 1 isoform X7
XM_016961798.1	XP_016817287.1	spectrin alpha chain, non-erythrocytic 1 isoform X10
XM_016961797.2	XP_016817286.2	spectrin alpha chain, non-erythrocytic 1 isoform X5
XM_009457423.3	XP_009455698.3	spectrin alpha chain, non-erythrocytic 1 isoform X6
XM_009457425.3	XP_009455700.3	spectrin alpha chain, non-erythrocytic 1 isoform X8
XM_016961798.2	XP_016817287.2	spectrin alpha chain, non-erythrocytic 1 isoform X10
XM_009457429.3	XP_009455704.3	spectrin alpha chain, non-erythrocytic 1 isoform X11
XM_016961799.2	XP_016817288.2	spectrin alpha chain, non-erythrocytic 1 isoform X12
XM_009457424.2	XP_009455699.2	spectrin alpha chain, non-erythrocytic 1 isoform X7
XM_009457429.2	XP_009455704.2	spectrin alpha chain, non-erythrocytic 1 isoform X11
XM_016961799.1	XP_016817288.1	spectrin alpha chain, non-erythrocytic 1 isoform X12
XM_009457427.3	XP_009455702.3	spectrin alpha chain, non-erythrocytic 1 isoform X9
XM_009457426.3	XP_009455701.3	spectrin alpha chain, non-erythrocytic 1 isoform X13
XM_016961796.1	XP_016817285.1	spectrin alpha chain, non-erythrocytic 1 isoform X4
XM_016961797.1	XP_016817286.1	spectrin alpha chain, non-erythrocytic 1 isoform X5
XM_009457423.2	XP_009455698.2	spectrin alpha chain, non-erythrocytic 1 isoform X6
XM_009457418.3	XP_009455693.3	spectrin alpha chain, non-erythrocytic 1 isoform X1
XM_016961796.2	XP_016817285.2	spectrin alpha chain, non-erythrocytic 1 isoform X4
XM_009457424.3	XP_009455699.3	spectrin alpha chain, non-erythrocytic 1 isoform X7
XM_009457418.2	XP_009455693.2	spectrin alpha chain, non-erythrocytic 1 isoform X1
XM_009457419.2	XP_009455694.2	spectrin alpha chain, non-erythrocytic 1 isoform X2
XM_009457425.2	XP_009455700.2	spectrin alpha chain, non-erythrocytic 1 isoform X8
XM_009457427.2	XP_009455702.2	spectrin alpha chain, non-erythrocytic 1 isoform X9
XM_009457426.2	XP_009455701.2	spectrin alpha chain, non-erythrocytic 1 isoform X13
XM_009457419.3	XP_009455694.3	spectrin alpha chain, non-erythrocytic 1 isoform X2

Homologies

Rattus norvegicus (Norway rat)	Sptan1	NP_741984.2
Danio rerio (zebrafish)	spna2	NP_001091958.1
Caenorhabditis elegans (roundworm)	spc-1	NP_001257000.1
Xenopus tropicalis (tropical clawed frog)	sptan1	NP_001090674.1
Canis lupus familiaris (dog)	SPTAN1	XP_005625341.1
Bos taurus (cattle)	SPTAN1	NP_001107628.1
Mus musculus (house mouse)	Sptan1	NP_001171138.1
Gallus gallus (chicken)	SPTAN1	NP_001036003.1
Homo sapiens (human)	SPTAN1	NP_001123910.1
	SPTAN1	XP_002800011.1
Drosophila melanogaster (fruit fly)	alpha-Spec	NP_001261286.1
Pan troglodytes (chimpanzee)	SPTAN1	XP_003312345.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006686	XP_316724.1

The following SPTAN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPTAN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPc109757	XM_009457418.3 Latest version!	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X1, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	29-31	$1301.30 $1859.00
OPc109758	XM_009457419.3 Latest version!	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X2, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	29-31	$1301.30 $1859.00
OPc109759	XM_016961795.2 Latest version!	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X3, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	29-31	$1301.30 $1859.00
OPc109760	XM_016961796.2 Latest version!	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X4, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	29-31	$1301.30 $1859.00
OPc109761	XM_016961797.2 Latest version!	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X5, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	29-31	$1301.30 $1859.00
OPc109762	XM_009457423.3 Latest version!	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X6, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	29-31	$1301.30 $1859.00
OPc109763	XM_024345907.1 Latest version!	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X8, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	29-31	$1301.30 $1859.00
OPc109763	XM_009457424.3 Latest version!	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X7, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	29-31	$1301.30 $1859.00
OPc109764	XM_009457425.3 Latest version!	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X9, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	29-31	$1301.30 $1859.00
OPc109765	XM_009457427.3 Latest version!	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X10, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	29-31	$1301.30 $1859.00
OPc109766	XM_016961798.2 Latest version!	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X11, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	29-31	$1301.30 $1859.00
OPc109767	XM_009457429.3 Latest version!	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X12, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	29-31	$1301.30 $1859.00
OPc109768	XM_016961799.2 Latest version!	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X13, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	29-31	$1301.30 $1859.00
OPc109769	XM_009457426.3 Latest version!	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X14, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	29-31	$1301.30 $1859.00
OPc89629	XM_009457418.2 Latest version!	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X1, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	29-31	$1371.30 $1959.00
OPc89630	XM_009457419.2 Latest version!	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X2, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	29-31	$1371.30 $1959.00
OPc89631	XM_016961795.1 Latest version!	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X3, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	29-31	$1371.30 $1959.00
OPc89632	XM_016961796.1 Latest version!	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X4, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	29-31	$1371.30 $1959.00
OPc89633	XM_016961797.1 Latest version!	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X5, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	29-31	$1371.30 $1959.00
OPc89634	XM_009457423.2 Latest version!	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X6, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	29-31	$1371.30 $1959.00
OPc89635	XM_009457424.2 Latest version!	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X7, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	29-31	$1371.30 $1959.00
OPc89636	XM_009457425.2 Latest version!	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X8, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	29-31	$1371.30 $1959.00
OPc89637	XM_009457427.2 Latest version!	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X9, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	29-31	$1371.30 $1959.00
OPc89638	XM_016961798.1 Latest version!	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X10, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	29-31	$1371.30 $1959.00
OPc89639	XM_009457429.2 Latest version!	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X11, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	29-31	$1371.30 $1959.00
OPc89640	XM_016961799.1 Latest version!	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X12, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	29-31	$1371.30 $1959.00
OPc89641	XM_009457426.2 Latest version!	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X13, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	29-31	$1371.30 $1959.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPTAN1 ( XM_009457418.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009455693.3 Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPc109757
Clone ID Related Accession (Same CDS sequence)	XM_009457418.3
Accession Version	XM_009457418.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7518bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036888.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009457418.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAATTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAATG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA ACACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCCC TGCTAGAGGC AGATGTGGCT 
GCTCATCAGG ACCGAATTGA TGGCATTACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATATTGAGGA TGAGGAGACG TGGATTCGAG AGAAGGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCAGCT GCTTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AGACACGCAT AACTAAGGAG GCCGGCAGTG TATCTCTGCG TATGAAGCAG 
GTGGAAGAAC TATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAGGC TCTAAACACA GACAATTATG GACATGATCT TGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAAT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCGTTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAAATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACGGCAAG TGATGAGTCA TACAAGGATC CCACCAACAT CCAGAGCAAG 
CACCAGAAGC ACCAGGCTTT TGAAGCAGAG CTGCATGCCA ACGCTGACCG GATCCGTGGG 
GTTATCGACA TGGGCAACTC CCTCATTGAA CGTGGAGCCT GTGCCGGCAG TGAGGATGCT 
GTCAAGGCCC GCCTGGCTGC CTTAGCTGAC CAGTGGCAAT TCTTGGTGCA AAAGTCAGCG 
GAAAAGAGCC AGAAACTGAA AGAAGCCAAC AAGCAGCAGA ACTTCAACAC AGGGATCAAG 
GACTTTGACT TCTGGCTGTC TGAGGTGGAG GCCCTGCTGG CATCCGAAGA TTATGGCAAA 
GACCTGGCTT CTGTGAACAA CCTGCTGAAA AAGCATCAAC TGCTGGAAGC AGATATATCT 
GCCCATGAGG ATCGCCTGAA GGACCTGAAC AGCCAGGCAG ACAGCCTGAT GACCAGCAGT 
GCCTTCGACA CCTCCCAAGT AAAGGACAAG AGGGACACCA TCAACGGGCG CTTCCAGAAG 
ATCAAGAGCA TGGCGGCCTC CCGGCGAGCC AAGCTGAATG AATCCCATCG CCTGCACCAG 
TTCTTCCGGG ACATGGATGA CGAGGAGTCC TGGATCAAGG AGAAGAAGCT GCTGGTGGGC 
TCGGAGGACT ACGGCCGGGA CCTAACCGGC GTGCAGAACC TGAGGAAGAA GCACAAGCGC 
CTGGAAGCAG AACTGGCTGC GCATGAGCCG GCTATTCAGG GTGTCCTGGA CACTGGCAAG 
AAGCTGTCCG ATGATAACAC CATCGGGAAA GAGGAGATCC AGCAGCGGCT GGCGCAGTTT 
GTGGAGCACT GGAAAGAGCT GAAGCAGCTG GCAGCTGCCC GGGGTCAGCG GCTGGAAGAG 
TCCTTGGAAT ATCAGCAGTT TGTAGCCAAT GTGGAAGAGG AGGAAGCCTG GATCAATGAG 
AAAATGACCC TGGTGGCCAG CGAAGATTAT GGCGACACTC TTGCTGCCAT CCAGGGCTTA 
CTGAAGAAAC ATGAAGCTTT TGAGACAGAC TTCACCGTCC ACAAGGATCG CGTGAATGAC 
GTCTGCACCA ATGGACAAGA CCTCATTAAG AAGAACAATC ACCATGAGGA GAACATCTCT 
TCAAAGATGA AGGGCCTGAA CGGGAAAGTG TCAGACCTGG AGAAAGCTGC AGCCCAGAGA 
AAGGCGAAGC TGGATGAGAA CTCGGCCTTC CTTCAGTTCA ACTGGAAGGC GGACGTGGTG 
GAGTCCTGGA TCGGTGAAAA GGAGAACAGC TTGAAGACAG ATGATTATGG CCGAGACCTG 
TCTTCTGTGC AGACGCTCCT CACCAAACAG GAAACTTTTG ACGCTGGGCT GCAGGCCTTC 
CAGCAGGAAG GCATTGCCAA CATCACTGCC CTCAAAGATC AGCTTCTCGC CGCCAAACAC 
GTTCAGTCCA AGGCCATCGA GGCCCGGCAC GCCTCCCTCA TGAAGAGGTG GAGCCAGCTT 
CTGGCCAACT CAGCCGCCCG CAAGAAGAAG CTTCTGGAGG CGCAGAGTCA CTTCCGCAAG 
GTGGAGGACC TCTTCCTGAC CTTTGCCAAA AAGGCTTCTG CCTTCAACAG CTGGTTTGAA 
AATGCAGAGG AGGACTTAAC AGACCCCGTG CGCTGCAACT CCTTGGAAGA AATCAAAGCT 
TTGCGCGAGG CCCACGACGC CTTCCGCTCC TCCCTCAGCT CTGCCCAGGC TGACTTCAAC 
CAGCTGGCCG AGCTGGACCG CCAGATCAAG AGCTTCCGCG TAGCCTCCAA CCCCTACACC 
TGGTTTACCA TGGAGGCCCT GGAGGAGACC TGGAGGAACC TACAGAAAAT CATCAAGGAG 
AGGGAGCTGG AGCTGCAGAA GGAACAGCGG CGGCAGGAGG AGAACGACAA GCTGCGCCAG 
GAGTTTGCCC AGCACGCCAA CGCCTTCCAC CAGTGGATCC AAGAGACCAG GACATACCTC 
CTCGATGGCA TAGCATATCG TCGGGTCATT CGTGTCTATC AGTATGAAGT TGGGGATGAT 
CTGTCTGGAA GGTCCTGTAT GGTGGAAGAG TCGGGGACCC TCGAATCCCA GCTTGAAGCT 
ACCAAACGCA AGCACCAGGA AATCCGAGCC ATGAGAAGTC AGCTCAAAAA GATCGAGGAC 
CTGGGGGCCG CCATGGAGGA GGCCCTCATC CTGGACAACA AGTACACGGA GCACAGCACC 
GTGGGCCTCG CCCAGCAGTG GGACCAGCTG GACCAGCTGG GCATGCGCAT GCAGCACAAC 
CTGGAGCAGC AGATCCAGGC CAGGAACACA ACAGGTGTGA CTGAGGAGGC CCTCAAAGAA 
TTCAGCATGA TGTTTAAACA CTTTGACAAG GACAAGTCTG GCAGGCTGAA CCATCAGGAG 
TTCAAATCTT GCCTGCGCTC CCTGGGCTAT GACCTGCCCA TGGTGGAGGA AGGGGAACCT 
GACCCTGAGT TCGAGGCAAT CCTGGACACG GTGGATCCGA ACAGAGATGG CCATGTCTCC 
CTGCAAGAAT ACATGGCTTT CATGATCAGC CGCGAAACTG AGAACGTCAA GTCCAGCGAG 
GAGATTGAGA GCGCCTTCCG GGCCCTCAGC TCAGAGGGAA AGCCTTACGT GACCAAGGAG 
GAGCTCTACC AGAACCTGAC CCGGGAACAA GCCGACTACT GCGTCTCCCA CATGAAGCCC 
TACGTGGACG GCAAGGGCCG CGAGCTCCCC ACCGCGTTCG ACTACGTGGA GTTCACCCGC 
TCGCTTTTCG TGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009455693.3
CDS	121..7638
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDIEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRITKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_009457418.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009457418.3

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAATTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAATG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA ACACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCCC TGCTAGAGGC AGATGTGGCT 
GCTCATCAGG ACCGAATTGA TGGCATTACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATATTGAGGA TGAGGAGACG TGGATTCGAG AGAAGGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCAGCT GCTTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AGACACGCAT AACTAAGGAG GCCGGCAGTG TATCTCTGCG TATGAAGCAG 
GTGGAAGAAC TATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAGGC TCTAAACACA GACAATTATG GACATGATCT TGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAAT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCGTTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAAATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACGGCAAG TGATGAGTCA TACAAGGATC CCACCAACAT CCAGAGCAAG 
CACCAGAAGC ACCAGGCTTT TGAAGCAGAG CTGCATGCCA ACGCTGACCG GATCCGTGGG 
GTTATCGACA TGGGCAACTC CCTCATTGAA CGTGGAGCCT GTGCCGGCAG TGAGGATGCT 
GTCAAGGCCC GCCTGGCTGC CTTAGCTGAC CAGTGGCAAT TCTTGGTGCA AAAGTCAGCG 
GAAAAGAGCC AGAAACTGAA AGAAGCCAAC AAGCAGCAGA ACTTCAACAC AGGGATCAAG 
GACTTTGACT TCTGGCTGTC TGAGGTGGAG GCCCTGCTGG CATCCGAAGA TTATGGCAAA 
GACCTGGCTT CTGTGAACAA CCTGCTGAAA AAGCATCAAC TGCTGGAAGC AGATATATCT 
GCCCATGAGG ATCGCCTGAA GGACCTGAAC AGCCAGGCAG ACAGCCTGAT GACCAGCAGT 
GCCTTCGACA CCTCCCAAGT AAAGGACAAG AGGGACACCA TCAACGGGCG CTTCCAGAAG 
ATCAAGAGCA TGGCGGCCTC CCGGCGAGCC AAGCTGAATG AATCCCATCG CCTGCACCAG 
TTCTTCCGGG ACATGGATGA CGAGGAGTCC TGGATCAAGG AGAAGAAGCT GCTGGTGGGC 
TCGGAGGACT ACGGCCGGGA CCTAACCGGC GTGCAGAACC TGAGGAAGAA GCACAAGCGC 
CTGGAAGCAG AACTGGCTGC GCATGAGCCG GCTATTCAGG GTGTCCTGGA CACTGGCAAG 
AAGCTGTCCG ATGATAACAC CATCGGGAAA GAGGAGATCC AGCAGCGGCT GGCGCAGTTT 
GTGGAGCACT GGAAAGAGCT GAAGCAGCTG GCAGCTGCCC GGGGTCAGCG GCTGGAAGAG 
TCCTTGGAAT ATCAGCAGTT TGTAGCCAAT GTGGAAGAGG AGGAAGCCTG GATCAATGAG 
AAAATGACCC TGGTGGCCAG CGAAGATTAT GGCGACACTC TTGCTGCCAT CCAGGGCTTA 
CTGAAGAAAC ATGAAGCTTT TGAGACAGAC TTCACCGTCC ACAAGGATCG CGTGAATGAC 
GTCTGCACCA ATGGACAAGA CCTCATTAAG AAGAACAATC ACCATGAGGA GAACATCTCT 
TCAAAGATGA AGGGCCTGAA CGGGAAAGTG TCAGACCTGG AGAAAGCTGC AGCCCAGAGA 
AAGGCGAAGC TGGATGAGAA CTCGGCCTTC CTTCAGTTCA ACTGGAAGGC GGACGTGGTG 
GAGTCCTGGA TCGGTGAAAA GGAGAACAGC TTGAAGACAG ATGATTATGG CCGAGACCTG 
TCTTCTGTGC AGACGCTCCT CACCAAACAG GAAACTTTTG ACGCTGGGCT GCAGGCCTTC 
CAGCAGGAAG GCATTGCCAA CATCACTGCC CTCAAAGATC AGCTTCTCGC CGCCAAACAC 
GTTCAGTCCA AGGCCATCGA GGCCCGGCAC GCCTCCCTCA TGAAGAGGTG GAGCCAGCTT 
CTGGCCAACT CAGCCGCCCG CAAGAAGAAG CTTCTGGAGG CGCAGAGTCA CTTCCGCAAG 
GTGGAGGACC TCTTCCTGAC CTTTGCCAAA AAGGCTTCTG CCTTCAACAG CTGGTTTGAA 
AATGCAGAGG AGGACTTAAC AGACCCCGTG CGCTGCAACT CCTTGGAAGA AATCAAAGCT 
TTGCGCGAGG CCCACGACGC CTTCCGCTCC TCCCTCAGCT CTGCCCAGGC TGACTTCAAC 
CAGCTGGCCG AGCTGGACCG CCAGATCAAG AGCTTCCGCG TAGCCTCCAA CCCCTACACC 
TGGTTTACCA TGGAGGCCCT GGAGGAGACC TGGAGGAACC TACAGAAAAT CATCAAGGAG 
AGGGAGCTGG AGCTGCAGAA GGAACAGCGG CGGCAGGAGG AGAACGACAA GCTGCGCCAG 
GAGTTTGCCC AGCACGCCAA CGCCTTCCAC CAGTGGATCC AAGAGACCAG GACATACCTC 
CTCGATGGCA TAGCATATCG TCGGGTCATT CGTGTCTATC AGTATGAAGT TGGGGATGAT 
CTGTCTGGAA GGTCCTGTAT GGTGGAAGAG TCGGGGACCC TCGAATCCCA GCTTGAAGCT 
ACCAAACGCA AGCACCAGGA AATCCGAGCC ATGAGAAGTC AGCTCAAAAA GATCGAGGAC 
CTGGGGGCCG CCATGGAGGA GGCCCTCATC CTGGACAACA AGTACACGGA GCACAGCACC 
GTGGGCCTCG CCCAGCAGTG GGACCAGCTG GACCAGCTGG GCATGCGCAT GCAGCACAAC 
CTGGAGCAGC AGATCCAGGC CAGGAACACA ACAGGTGTGA CTGAGGAGGC CCTCAAAGAA 
TTCAGCATGA TGTTTAAACA CTTTGACAAG GACAAGTCTG GCAGGCTGAA CCATCAGGAG 
TTCAAATCTT GCCTGCGCTC CCTGGGCTAT GACCTGCCCA TGGTGGAGGA AGGGGAACCT 
GACCCTGAGT TCGAGGCAAT CCTGGACACG GTGGATCCGA ACAGAGATGG CCATGTCTCC 
CTGCAAGAAT ACATGGCTTT CATGATCAGC CGCGAAACTG AGAACGTCAA GTCCAGCGAG 
GAGATTGAGA GCGCCTTCCG GGCCCTCAGC TCAGAGGGAA AGCCTTACGT GACCAAGGAG 
GAGCTCTACC AGAACCTGAC CCGGGAACAA GCCGACTACT GCGTCTCCCA CATGAAGCCC 
TACGTGGACG GCAAGGGCCG CGAGCTCCCC ACCGCGTTCG ACTACGTGGA GTTCACCCGC 
TCGCTTTTCG TGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_009457419.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009455694.3 Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X2, mRNA.

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CloneID	OPc109758
Clone ID Related Accession (Same CDS sequence)	XM_009457419.3
Accession Version	XM_009457419.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7497bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036888.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009457419.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAA TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AATGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAACACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCCCTGCTAG AGGCAGATGT GGCTGCTCAT CAGGACCGAA TTGATGGCAT TACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATATTG AGGATGAGGA GACGTGGATT 
CGAGAGAAGG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC AGCTGCTTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAGACAC GCATAACTAA GGAGGCCGGC 
AGTGTATCTC TGCGTATGAA GCAGGTGGAA GAACTATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AGGCTCTAAA CACAGACAAT 
TATGGACATG ATCTTGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAATCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCGTT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAAATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACGG CAAGTGATGA GTCATACAAG 
GATCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAGGCTTTT 
GAAGCAGAGC TGCATGCCAA CGCTGACCGG ATCCGTGGGG TTATCGACAT GGGCAACTCC 
CTCATTGAAC GTGGAGCCTG TGCCGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
TTAGCTGACC AGTGGCAATT CTTGGTGCAA AAGTCAGCGG AAAAGAGCCA GAAACTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACA GGGATCAAGG ACTTTGACTT CTGGCTGTCT 
GAGGTGGAGG CCCTGCTGGC ATCCGAAGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC 
CTGCTGAAAA AGCATCAACT GCTGGAAGCA GATATATCTG CCCATGAGGA TCGCCTGAAG 
GACCTGAACA GCCAGGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTA 
AAGGACAAGA GGGACACCAT CAACGGGCGC TTCCAGAAGA TCAAGAGCAT GGCGGCCTCC 
CGGCGAGCCA AGCTGAATGA ATCCCATCGC CTGCACCAGT TCTTCCGGGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGGGCT CGGAGGACTA CGGCCGGGAC 
CTAACCGGCG TGCAGAACCT GAGGAAGAAG CACAAGCGCC TGGAAGCAGA ACTGGCTGCG 
CATGAGCCGG CTATTCAGGG TGTCCTGGAC ACTGGCAAGA AGCTGTCCGA TGATAACACC 
ATCGGGAAAG AGGAGATCCA GCAGCGGCTG GCGCAGTTTG TGGAGCACTG GAAAGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGTCAGCGG CTGGAAGAGT CCTTGGAATA TCAGCAGTTT 
GTAGCCAATG TGGAAGAGGA GGAAGCCTGG ATCAATGAGA AAATGACCCT GGTGGCCAGC 
GAAGATTATG GCGACACTCT TGCTGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACCGTCCA CAAGGATCGC GTGAATGACG TCTGCACCAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGCCTGAAC 
GGGAAAGTGT CAGACCTGGA GAAAGCTGCA GCCCAGAGAA AGGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA CTGGAAGGCG GACGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGC CGAGACCTGT CTTCTGTGCA GACGCTCCTC 
ACCAAACAGG AAACTTTTGA CGCTGGGCTG CAGGCCTTCC AGCAGGAAGG CATTGCCAAC 
ATCACTGCCC TCAAAGATCA GCTTCTCGCC GCCAAACACG TTCAGTCCAA GGCCATCGAG 
GCCCGGCACG CCTCCCTCAT GAAGAGGTGG AGCCAGCTTC TGGCCAACTC AGCCGCCCGC 
AAGAAGAAGC TTCTGGAGGC GCAGAGTCAC TTCCGCAAGG TGGAGGACCT CTTCCTGACC 
TTTGCCAAAA AGGCTTCTGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCCGTGC GCTGCAACTC CTTGGAAGAA ATCAAAGCTT TGCGCGAGGC CCACGACGCC 
TTCCGCTCCT CCCTCAGCTC TGCCCAGGCT GACTTCAACC AGCTGGCCGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT AGCCTCCAAC CCCTACACCT GGTTTACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT ACAGAAAATC ATCAAGGAGA GGGAGCTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTTGCCCA GCACGCCAAC 
GCCTTCCACC AGTGGATCCA AGAGACCAGG ACATACCTCC TCGATGGCAT AGCATATCGT 
CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGTATG 
GTGGAAGAGT CGGGGACCCT CGAATCCCAG CTTGAAGCTA CCAAACGCAA GCACCAGGAA 
ATCCGAGCCA TGAGAAGTCA GCTCAAAAAG ATCGAGGACC TGGGGGCCGC CATGGAGGAG 
GCCCTCATCC TGGACAACAA GTACACGGAG CACAGCACCG TGGGCCTCGC CCAGCAGTGG 
GACCAGCTGG ACCAGCTGGG CATGCGCATG CAGCACAACC TGGAGCAGCA GATCCAGGCC 
AGGAACACAA CAGGTGTGAC TGAGGAGGCC CTCAAAGAAT TCAGCATGAT GTTTAAACAC 
TTTGACAAGG ACAAGTCTGG CAGGCTGAAC CATCAGGAGT TCAAATCTTG CCTGCGCTCC 
CTGGGCTATG ACCTGCCCAT GGTGGAGGAA GGGGAACCTG ACCCTGAGTT CGAGGCAATC 
CTGGACACGG TGGATCCGAA CAGAGATGGC CATGTCTCCC TGCAAGAATA CATGGCTTTC 
ATGATCAGCC GCGAAACTGA GAACGTCAAG TCCAGCGAGG AGATTGAGAG CGCCTTCCGG 
GCCCTCAGCT CAGAGGGAAA GCCTTACGTG ACCAAGGAGG AGCTCTACCA GAACCTGACC 
CGGGAACAAG CCGACTACTG CGTCTCCCAC ATGAAGCCCT ACGTGGACGG CAAGGGCCGC 
GAGCTCCCCA CCGCGTTCGA CTACGTGGAG TTCACCCGCT CGCTTTTCGT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009455694.3
CDS	138..7634
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDIEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRITKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_009457419.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009457419.3

ORF Insert Sequence:

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAA TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AATGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAACACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCCCTGCTAG AGGCAGATGT GGCTGCTCAT CAGGACCGAA TTGATGGCAT TACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATATTG AGGATGAGGA GACGTGGATT 
CGAGAGAAGG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC AGCTGCTTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAGACAC GCATAACTAA GGAGGCCGGC 
AGTGTATCTC TGCGTATGAA GCAGGTGGAA GAACTATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AGGCTCTAAA CACAGACAAT 
TATGGACATG ATCTTGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAATCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCGTT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAAATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACGG CAAGTGATGA GTCATACAAG 
GATCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAGGCTTTT 
GAAGCAGAGC TGCATGCCAA CGCTGACCGG ATCCGTGGGG TTATCGACAT GGGCAACTCC 
CTCATTGAAC GTGGAGCCTG TGCCGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
TTAGCTGACC AGTGGCAATT CTTGGTGCAA AAGTCAGCGG AAAAGAGCCA GAAACTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACA GGGATCAAGG ACTTTGACTT CTGGCTGTCT 
GAGGTGGAGG CCCTGCTGGC ATCCGAAGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC 
CTGCTGAAAA AGCATCAACT GCTGGAAGCA GATATATCTG CCCATGAGGA TCGCCTGAAG 
GACCTGAACA GCCAGGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTA 
AAGGACAAGA GGGACACCAT CAACGGGCGC TTCCAGAAGA TCAAGAGCAT GGCGGCCTCC 
CGGCGAGCCA AGCTGAATGA ATCCCATCGC CTGCACCAGT TCTTCCGGGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGGGCT CGGAGGACTA CGGCCGGGAC 
CTAACCGGCG TGCAGAACCT GAGGAAGAAG CACAAGCGCC TGGAAGCAGA ACTGGCTGCG 
CATGAGCCGG CTATTCAGGG TGTCCTGGAC ACTGGCAAGA AGCTGTCCGA TGATAACACC 
ATCGGGAAAG AGGAGATCCA GCAGCGGCTG GCGCAGTTTG TGGAGCACTG GAAAGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGTCAGCGG CTGGAAGAGT CCTTGGAATA TCAGCAGTTT 
GTAGCCAATG TGGAAGAGGA GGAAGCCTGG ATCAATGAGA AAATGACCCT GGTGGCCAGC 
GAAGATTATG GCGACACTCT TGCTGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACCGTCCA CAAGGATCGC GTGAATGACG TCTGCACCAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGCCTGAAC 
GGGAAAGTGT CAGACCTGGA GAAAGCTGCA GCCCAGAGAA AGGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA CTGGAAGGCG GACGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGC CGAGACCTGT CTTCTGTGCA GACGCTCCTC 
ACCAAACAGG AAACTTTTGA CGCTGGGCTG CAGGCCTTCC AGCAGGAAGG CATTGCCAAC 
ATCACTGCCC TCAAAGATCA GCTTCTCGCC GCCAAACACG TTCAGTCCAA GGCCATCGAG 
GCCCGGCACG CCTCCCTCAT GAAGAGGTGG AGCCAGCTTC TGGCCAACTC AGCCGCCCGC 
AAGAAGAAGC TTCTGGAGGC GCAGAGTCAC TTCCGCAAGG TGGAGGACCT CTTCCTGACC 
TTTGCCAAAA AGGCTTCTGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCCGTGC GCTGCAACTC CTTGGAAGAA ATCAAAGCTT TGCGCGAGGC CCACGACGCC 
TTCCGCTCCT CCCTCAGCTC TGCCCAGGCT GACTTCAACC AGCTGGCCGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT AGCCTCCAAC CCCTACACCT GGTTTACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT ACAGAAAATC ATCAAGGAGA GGGAGCTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTTGCCCA GCACGCCAAC 
GCCTTCCACC AGTGGATCCA AGAGACCAGG ACATACCTCC TCGATGGCAT AGCATATCGT 
CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGTATG 
GTGGAAGAGT CGGGGACCCT CGAATCCCAG CTTGAAGCTA CCAAACGCAA GCACCAGGAA 
ATCCGAGCCA TGAGAAGTCA GCTCAAAAAG ATCGAGGACC TGGGGGCCGC CATGGAGGAG 
GCCCTCATCC TGGACAACAA GTACACGGAG CACAGCACCG TGGGCCTCGC CCAGCAGTGG 
GACCAGCTGG ACCAGCTGGG CATGCGCATG CAGCACAACC TGGAGCAGCA GATCCAGGCC 
AGGAACACAA CAGGTGTGAC TGAGGAGGCC CTCAAAGAAT TCAGCATGAT GTTTAAACAC 
TTTGACAAGG ACAAGTCTGG CAGGCTGAAC CATCAGGAGT TCAAATCTTG CCTGCGCTCC 
CTGGGCTATG ACCTGCCCAT GGTGGAGGAA GGGGAACCTG ACCCTGAGTT CGAGGCAATC 
CTGGACACGG TGGATCCGAA CAGAGATGGC CATGTCTCCC TGCAAGAATA CATGGCTTTC 
ATGATCAGCC GCGAAACTGA GAACGTCAAG TCCAGCGAGG AGATTGAGAG CGCCTTCCGG 
GCCCTCAGCT CAGAGGGAAA GCCTTACGTG ACCAAGGAGG AGCTCTACCA GAACCTGACC 
CGGGAACAAG CCGACTACTG CGTCTCCCAC ATGAAGCCCT ACGTGGACGG CAAGGGCCGC 
GAGCTCCCCA CCGCGTTCGA CTACGTGGAG TTCACCCGCT CGCTTTTCGT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_016961795.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016817284.2 Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X3, mRNA.

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CloneID	OPc109759
Clone ID Related Accession (Same CDS sequence)	XM_016961795.2
Accession Version	XM_016961795.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7473bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036888.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016961795.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAATTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAATG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA ACACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCCC TGCTAGAGGC AGATGTGGCT 
GCTCATCAGG ACCGAATTGA TGGCATTACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATATTGAGGA TGAGGAGACG TGGATTCGAG AGAAGGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCAGCT GCTTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAGGC TCTAAACACA GACAATTATG GACATGATCT TGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAAT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCGTTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAAATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACGGCAAG TGATGAGTCA TACAAGGATC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCACCA GAAGCACCAG GCTTTTGAAG CAGAGCTGCA TGCCAACGCT 
GACCGGATCC GTGGGGTTAT CGACATGGGC AACTCCCTCA TTGAACGTGG AGCCTGTGCC 
GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCTTAG CTGACCAGTG GCAATTCTTG 
GTGCAAAAGT CAGCGGAAAA GAGCCAGAAA CTGAAAGAAG CCAACAAGCA GCAGAACTTC 
AACACAGGGA TCAAGGACTT TGACTTCTGG CTGTCTGAGG TGGAGGCCCT GCTGGCATCC 
GAAGATTATG GCAAAGACCT GGCTTCTGTG AACAACCTGC TGAAAAAGCA TCAACTGCTG 
GAAGCAGATA TATCTGCCCA TGAGGATCGC CTGAAGGACC TGAACAGCCA GGCAGACAGC 
CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTAAAGG ACAAGAGGGA CACCATCAAC 
GGGCGCTTCC AGAAGATCAA GAGCATGGCG GCCTCCCGGC GAGCCAAGCT GAATGAATCC 
CATCGCCTGC ACCAGTTCTT CCGGGACATG GATGACGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGGGCTCGGA GGACTACGGC CGGGACCTAA CCGGCGTGCA GAACCTGAGG 
AAGAAGCACA AGCGCCTGGA AGCAGAACTG GCTGCGCATG AGCCGGCTAT TCAGGGTGTC 
CTGGACACTG GCAAGAAGCT GTCCGATGAT AACACCATCG GGAAAGAGGA GATCCAGCAG 
CGGCTGGCGC AGTTTGTGGA GCACTGGAAA GAGCTGAAGC AGCTGGCAGC TGCCCGGGGT 
CAGCGGCTGG AAGAGTCCTT GGAATATCAG CAGTTTGTAG CCAATGTGGA AGAGGAGGAA 
GCCTGGATCA ATGAGAAAAT GACCCTGGTG GCCAGCGAAG ATTATGGCGA CACTCTTGCT 
GCCATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC CGTCCACAAG 
GATCGCGTGA ATGACGTCTG CACCAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCAAA GATGAAGGGC CTGAACGGGA AAGTGTCAGA CCTGGAGAAA 
GCTGCAGCCC AGAGAAAGGC GAAGCTGGAT GAGAACTCGG CCTTCCTTCA GTTCAACTGG 
AAGGCGGACG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGCCGAG ACCTGTCTTC TGTGCAGACG CTCCTCACCA AACAGGAAAC TTTTGACGCT 
GGGCTGCAGG CCTTCCAGCA GGAAGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTT 
CTCGCCGCCA AACACGTTCA GTCCAAGGCC ATCGAGGCCC GGCACGCCTC CCTCATGAAG 
AGGTGGAGCC AGCTTCTGGC CAACTCAGCC GCCCGCAAGA AGAAGCTTCT GGAGGCGCAG 
AGTCACTTCC GCAAGGTGGA GGACCTCTTC CTGACCTTTG CCAAAAAGGC TTCTGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CCGTGCGCTG CAACTCCTTG 
GAAGAAATCA AAGCTTTGCG CGAGGCCCAC GACGCCTTCC GCTCCTCCCT CAGCTCTGCC 
CAGGCTGACT TCAACCAGCT GGCCGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTAGCC 
TCCAACCCCT ACACCTGGTT TACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTACAG 
AAAATCATCA AGGAGAGGGA GCTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT TGCCCAGCAC GCCAACGCCT TCCACCAGTG GATCCAAGAG 
ACCAGGACAT ACCTCCTCGA TGGCATAGCA TATCGTCGGG TCATTCGTGT CTATCAGTAT 
GAAGTTGGGG ATGATCTGTC TGGAAGGTCC TGTATGGTGG AAGAGTCGGG GACCCTCGAA 
TCCCAGCTTG AAGCTACCAA ACGCAAGCAC CAGGAAATCC GAGCCATGAG AAGTCAGCTC 
AAAAAGATCG AGGACCTGGG GGCCGCCATG GAGGAGGCCC TCATCCTGGA CAACAAGTAC 
ACGGAGCACA GCACCGTGGG CCTCGCCCAG CAGTGGGACC AGCTGGACCA GCTGGGCATG 
CGCATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACAACAGG TGTGACTGAG 
GAGGCCCTCA AAGAATTCAG CATGATGTTT AAACACTTTG ACAAGGACAA GTCTGGCAGG 
CTGAACCATC AGGAGTTCAA ATCTTGCCTG CGCTCCCTGG GCTATGACCT GCCCATGGTG 
GAGGAAGGGG AACCTGACCC TGAGTTCGAG GCAATCCTGG ACACGGTGGA TCCGAACAGA 
GATGGCCATG TCTCCCTGCA AGAATACATG GCTTTCATGA TCAGCCGCGA AACTGAGAAC 
GTCAAGTCCA GCGAGGAGAT TGAGAGCGCC TTCCGGGCCC TCAGCTCAGA GGGAAAGCCT 
TACGTGACCA AGGAGGAGCT CTACCAGAAC CTGACCCGGG AACAAGCCGA CTACTGCGTC 
TCCCACATGA AGCCCTACGT GGACGGCAAG GGCCGCGAGC TCCCCACCGC GTTCGACTAC 
GTGGAGTTCA CCCGCTCGCT TTTCGTGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016817284.2
CDS	121..7593
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDIEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_016961795.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016961795.2

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAATTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAATG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA ACACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCCC TGCTAGAGGC AGATGTGGCT 
GCTCATCAGG ACCGAATTGA TGGCATTACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATATTGAGGA TGAGGAGACG TGGATTCGAG AGAAGGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCAGCT GCTTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAGGC TCTAAACACA GACAATTATG GACATGATCT TGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAAT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCGTTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAAATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACGGCAAG TGATGAGTCA TACAAGGATC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCACCA GAAGCACCAG GCTTTTGAAG CAGAGCTGCA TGCCAACGCT 
GACCGGATCC GTGGGGTTAT CGACATGGGC AACTCCCTCA TTGAACGTGG AGCCTGTGCC 
GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCTTAG CTGACCAGTG GCAATTCTTG 
GTGCAAAAGT CAGCGGAAAA GAGCCAGAAA CTGAAAGAAG CCAACAAGCA GCAGAACTTC 
AACACAGGGA TCAAGGACTT TGACTTCTGG CTGTCTGAGG TGGAGGCCCT GCTGGCATCC 
GAAGATTATG GCAAAGACCT GGCTTCTGTG AACAACCTGC TGAAAAAGCA TCAACTGCTG 
GAAGCAGATA TATCTGCCCA TGAGGATCGC CTGAAGGACC TGAACAGCCA GGCAGACAGC 
CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTAAAGG ACAAGAGGGA CACCATCAAC 
GGGCGCTTCC AGAAGATCAA GAGCATGGCG GCCTCCCGGC GAGCCAAGCT GAATGAATCC 
CATCGCCTGC ACCAGTTCTT CCGGGACATG GATGACGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGGGCTCGGA GGACTACGGC CGGGACCTAA CCGGCGTGCA GAACCTGAGG 
AAGAAGCACA AGCGCCTGGA AGCAGAACTG GCTGCGCATG AGCCGGCTAT TCAGGGTGTC 
CTGGACACTG GCAAGAAGCT GTCCGATGAT AACACCATCG GGAAAGAGGA GATCCAGCAG 
CGGCTGGCGC AGTTTGTGGA GCACTGGAAA GAGCTGAAGC AGCTGGCAGC TGCCCGGGGT 
CAGCGGCTGG AAGAGTCCTT GGAATATCAG CAGTTTGTAG CCAATGTGGA AGAGGAGGAA 
GCCTGGATCA ATGAGAAAAT GACCCTGGTG GCCAGCGAAG ATTATGGCGA CACTCTTGCT 
GCCATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC CGTCCACAAG 
GATCGCGTGA ATGACGTCTG CACCAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCAAA GATGAAGGGC CTGAACGGGA AAGTGTCAGA CCTGGAGAAA 
GCTGCAGCCC AGAGAAAGGC GAAGCTGGAT GAGAACTCGG CCTTCCTTCA GTTCAACTGG 
AAGGCGGACG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGCCGAG ACCTGTCTTC TGTGCAGACG CTCCTCACCA AACAGGAAAC TTTTGACGCT 
GGGCTGCAGG CCTTCCAGCA GGAAGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTT 
CTCGCCGCCA AACACGTTCA GTCCAAGGCC ATCGAGGCCC GGCACGCCTC CCTCATGAAG 
AGGTGGAGCC AGCTTCTGGC CAACTCAGCC GCCCGCAAGA AGAAGCTTCT GGAGGCGCAG 
AGTCACTTCC GCAAGGTGGA GGACCTCTTC CTGACCTTTG CCAAAAAGGC TTCTGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CCGTGCGCTG CAACTCCTTG 
GAAGAAATCA AAGCTTTGCG CGAGGCCCAC GACGCCTTCC GCTCCTCCCT CAGCTCTGCC 
CAGGCTGACT TCAACCAGCT GGCCGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTAGCC 
TCCAACCCCT ACACCTGGTT TACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTACAG 
AAAATCATCA AGGAGAGGGA GCTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT TGCCCAGCAC GCCAACGCCT TCCACCAGTG GATCCAAGAG 
ACCAGGACAT ACCTCCTCGA TGGCATAGCA TATCGTCGGG TCATTCGTGT CTATCAGTAT 
GAAGTTGGGG ATGATCTGTC TGGAAGGTCC TGTATGGTGG AAGAGTCGGG GACCCTCGAA 
TCCCAGCTTG AAGCTACCAA ACGCAAGCAC CAGGAAATCC GAGCCATGAG AAGTCAGCTC 
AAAAAGATCG AGGACCTGGG GGCCGCCATG GAGGAGGCCC TCATCCTGGA CAACAAGTAC 
ACGGAGCACA GCACCGTGGG CCTCGCCCAG CAGTGGGACC AGCTGGACCA GCTGGGCATG 
CGCATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACAACAGG TGTGACTGAG 
GAGGCCCTCA AAGAATTCAG CATGATGTTT AAACACTTTG ACAAGGACAA GTCTGGCAGG 
CTGAACCATC AGGAGTTCAA ATCTTGCCTG CGCTCCCTGG GCTATGACCT GCCCATGGTG 
GAGGAAGGGG AACCTGACCC TGAGTTCGAG GCAATCCTGG ACACGGTGGA TCCGAACAGA 
GATGGCCATG TCTCCCTGCA AGAATACATG GCTTTCATGA TCAGCCGCGA AACTGAGAAC 
GTCAAGTCCA GCGAGGAGAT TGAGAGCGCC TTCCGGGCCC TCAGCTCAGA GGGAAAGCCT 
TACGTGACCA AGGAGGAGCT CTACCAGAAC CTGACCCGGG AACAAGCCGA CTACTGCGTC 
TCCCACATGA AGCCCTACGT GGACGGCAAG GGCCGCGAGC TCCCCACCGC GTTCGACTAC 
GTGGAGTTCA CCCGCTCGCT TTTCGTGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_016961796.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016817285.2 Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X4, mRNA.

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CloneID	OPc109760
Clone ID Related Accession (Same CDS sequence)	XM_016961796.2
Accession Version	XM_016961796.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7470bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036888.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016961796.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAATTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAATG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA ACACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCCC TGCTAGAGGC AGATGTGGCT 
GCTCATCAGG ACCGAATTGA TGGCATTACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATATTGAGGA TGAGGAGACG TGGATTCGAG AGAAGGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCAGCT GCTTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AGACACGCAT AACTAAGGAG GCCGGCAGTG TATCTCTGCG TATGAAGCAG 
GTGGAAGAAC TATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAGGC TCTAAACACA GACAATTATG GACATGATCT TGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAAT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCGTTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAAATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACGGCAAG TGATGAGTCA TACAAGGATC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCACCA GAAGCACCAG GCTTTTGAAG CAGAGCTGCA TGCCAACGCT 
GACCGGATCC GTGGGGTTAT CGACATGGGC AACTCCCTCA TTGAACGTGG AGCCTGTGCC 
GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCTTAG CTGACCAGTG GCAATTCTTG 
GTGCAAAAGT CAGCGGAAAA GAGCCAGAAA CTGAAAGAAG CCAACAAGCA GCAGAACTTC 
AACACAGGGA TCAAGGACTT TGACTTCTGG CTGTCTGAGG TGGAGGCCCT GCTGGCATCC 
GAAGATTATG GCAAAGACCT GGCTTCTGTG AACAACCTGC TGAAAAAGCA TCAACTGCTG 
GAAGCAGATA TATCTGCCCA TGAGGATCGC CTGAAGGACC TGAACAGCCA GGCAGACAGC 
CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTAAAGG ACAAGAGGGA CACCATCAAC 
GGGCGCTTCC AGAAGATCAA GAGCATGGCG GCCTCCCGGC GAGCCAAGCT GAATGAATCC 
CATCGCCTGC ACCAGTTCTT CCGGGACATG GATGACGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGGGCTCGGA GGACTACGGC CGGGACCTAA CCGGCGTGCA GAACCTGAGG 
AAGAAGCACA AGCGCCTGGA AGCAGAACTG GCTGCGCATG AGCCGGCTAT TCAGGGTGTC 
CTGGACACTG GCAAGAAGCT GTCCGATGAT AACACCATCG GGAAAGAGGA GATCCAGCAG 
CGGCTGGCGC AGTTTGTGGA GCACTGGAAA GAGCTGAAGC AGCTGGCAGC TGCCCGGGGT 
CAGCGGCTGG AAGAGTCCTT GGAATATCAG CAGTTTGTAG CCAATGTGGA AGAGGAGGAA 
GCCTGGATCA ATGAGAAAAT GACCCTGGTG GCCAGCGAAG ATTATGGCGA CACTCTTGCT 
GCCATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC CGTCCACAAG 
GATCGCGTGA ATGACGTCTG CACCAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCAAA GATGAAGGGC CTGAACGGGA AAGTGTCAGA CCTGGAGAAA 
GCTGCAGCCC AGAGAAAGGC GAAGCTGGAT GAGAACTCGG CCTTCCTTCA GTTCAACTGG 
AAGGCGGACG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGCCGAG ACCTGTCTTC TGTGCAGACG CTCCTCACCA AACAGGAAAC TTTTGACGCT 
GGGCTGCAGG CCTTCCAGCA GGAAGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTT 
CTCGCCGCCA AACACGTTCA GTCCAAGGCC ATCGAGGCCC GGCACGCCTC CCTCATGAAG 
AGGTGGAGCC AGCTTCTGGC CAACTCAGCC GCCCGCAAGA AGAAGCTTCT GGAGGCGCAG 
AGTCACTTCC GCAAGGTGGA GGACCTCTTC CTGACCTTTG CCAAAAAGGC TTCTGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CCGTGCGCTG CAACTCCTTG 
GAAGAAATCA AAGCTTTGCG CGAGGCCCAC GACGCCTTCC GCTCCTCCCT CAGCTCTGCC 
CAGGCTGACT TCAACCAGCT GGCCGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTAGCC 
TCCAACCCCT ACACCTGGTT TACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTACAG 
AAAATCATCA AGGAGAGGGA GCTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT TGCCCAGCAC GCCAACGCCT TCCACCAGTG GATCCAAGAG 
ACCAGGACAT ACCTCCTCGA TGGGTCCTGT ATGGTGGAAG AGTCGGGGAC CCTCGAATCC 
CAGCTTGAAG CTACCAAACG CAAGCACCAG GAAATCCGAG CCATGAGAAG TCAGCTCAAA 
AAGATCGAGG ACCTGGGGGC CGCCATGGAG GAGGCCCTCA TCCTGGACAA CAAGTACACG 
GAGCACAGCA CCGTGGGCCT CGCCCAGCAG TGGGACCAGC TGGACCAGCT GGGCATGCGC 
ATGCAGCACA ACCTGGAGCA GCAGATCCAG GCCAGGAACA CAACAGGTGT GACTGAGGAG 
GCCCTCAAAG AATTCAGCAT GATGTTTAAA CACTTTGACA AGGACAAGTC TGGCAGGCTG 
AACCATCAGG AGTTCAAATC TTGCCTGCGC TCCCTGGGCT ATGACCTGCC CATGGTGGAG 
GAAGGGGAAC CTGACCCTGA GTTCGAGGCA ATCCTGGACA CGGTGGATCC GAACAGAGAT 
GGCCATGTCT CCCTGCAAGA ATACATGGCT TTCATGATCA GCCGCGAAAC TGAGAACGTC 
AAGTCCAGCG AGGAGATTGA GAGCGCCTTC CGGGCCCTCA GCTCAGAGGG AAAGCCTTAC 
GTGACCAAGG AGGAGCTCTA CCAGAACCTG ACCCGGGAAC AAGCCGACTA CTGCGTCTCC 
CACATGAAGC CCTACGTGGA CGGCAAGGGC CGCGAGCTCC CCACCGCGTT CGACTACGTG 
GAGTTCACCC GCTCGCTTTT CGTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016817285.2
CDS	121..7590
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDIEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRITKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_016961796.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016961796.2

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAATTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAATG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA ACACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCCC TGCTAGAGGC AGATGTGGCT 
GCTCATCAGG ACCGAATTGA TGGCATTACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATATTGAGGA TGAGGAGACG TGGATTCGAG AGAAGGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCAGCT GCTTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AGACACGCAT AACTAAGGAG GCCGGCAGTG TATCTCTGCG TATGAAGCAG 
GTGGAAGAAC TATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAGGC TCTAAACACA GACAATTATG GACATGATCT TGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAAT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCGTTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAAATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACGGCAAG TGATGAGTCA TACAAGGATC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCACCA GAAGCACCAG GCTTTTGAAG CAGAGCTGCA TGCCAACGCT 
GACCGGATCC GTGGGGTTAT CGACATGGGC AACTCCCTCA TTGAACGTGG AGCCTGTGCC 
GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCTTAG CTGACCAGTG GCAATTCTTG 
GTGCAAAAGT CAGCGGAAAA GAGCCAGAAA CTGAAAGAAG CCAACAAGCA GCAGAACTTC 
AACACAGGGA TCAAGGACTT TGACTTCTGG CTGTCTGAGG TGGAGGCCCT GCTGGCATCC 
GAAGATTATG GCAAAGACCT GGCTTCTGTG AACAACCTGC TGAAAAAGCA TCAACTGCTG 
GAAGCAGATA TATCTGCCCA TGAGGATCGC CTGAAGGACC TGAACAGCCA GGCAGACAGC 
CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTAAAGG ACAAGAGGGA CACCATCAAC 
GGGCGCTTCC AGAAGATCAA GAGCATGGCG GCCTCCCGGC GAGCCAAGCT GAATGAATCC 
CATCGCCTGC ACCAGTTCTT CCGGGACATG GATGACGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGGGCTCGGA GGACTACGGC CGGGACCTAA CCGGCGTGCA GAACCTGAGG 
AAGAAGCACA AGCGCCTGGA AGCAGAACTG GCTGCGCATG AGCCGGCTAT TCAGGGTGTC 
CTGGACACTG GCAAGAAGCT GTCCGATGAT AACACCATCG GGAAAGAGGA GATCCAGCAG 
CGGCTGGCGC AGTTTGTGGA GCACTGGAAA GAGCTGAAGC AGCTGGCAGC TGCCCGGGGT 
CAGCGGCTGG AAGAGTCCTT GGAATATCAG CAGTTTGTAG CCAATGTGGA AGAGGAGGAA 
GCCTGGATCA ATGAGAAAAT GACCCTGGTG GCCAGCGAAG ATTATGGCGA CACTCTTGCT 
GCCATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC CGTCCACAAG 
GATCGCGTGA ATGACGTCTG CACCAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCAAA GATGAAGGGC CTGAACGGGA AAGTGTCAGA CCTGGAGAAA 
GCTGCAGCCC AGAGAAAGGC GAAGCTGGAT GAGAACTCGG CCTTCCTTCA GTTCAACTGG 
AAGGCGGACG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGCCGAG ACCTGTCTTC TGTGCAGACG CTCCTCACCA AACAGGAAAC TTTTGACGCT 
GGGCTGCAGG CCTTCCAGCA GGAAGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTT 
CTCGCCGCCA AACACGTTCA GTCCAAGGCC ATCGAGGCCC GGCACGCCTC CCTCATGAAG 
AGGTGGAGCC AGCTTCTGGC CAACTCAGCC GCCCGCAAGA AGAAGCTTCT GGAGGCGCAG 
AGTCACTTCC GCAAGGTGGA GGACCTCTTC CTGACCTTTG CCAAAAAGGC TTCTGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CCGTGCGCTG CAACTCCTTG 
GAAGAAATCA AAGCTTTGCG CGAGGCCCAC GACGCCTTCC GCTCCTCCCT CAGCTCTGCC 
CAGGCTGACT TCAACCAGCT GGCCGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTAGCC 
TCCAACCCCT ACACCTGGTT TACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTACAG 
AAAATCATCA AGGAGAGGGA GCTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT TGCCCAGCAC GCCAACGCCT TCCACCAGTG GATCCAAGAG 
ACCAGGACAT ACCTCCTCGA TGGGTCCTGT ATGGTGGAAG AGTCGGGGAC CCTCGAATCC 
CAGCTTGAAG CTACCAAACG CAAGCACCAG GAAATCCGAG CCATGAGAAG TCAGCTCAAA 
AAGATCGAGG ACCTGGGGGC CGCCATGGAG GAGGCCCTCA TCCTGGACAA CAAGTACACG 
GAGCACAGCA CCGTGGGCCT CGCCCAGCAG TGGGACCAGC TGGACCAGCT GGGCATGCGC 
ATGCAGCACA ACCTGGAGCA GCAGATCCAG GCCAGGAACA CAACAGGTGT GACTGAGGAG 
GCCCTCAAAG AATTCAGCAT GATGTTTAAA CACTTTGACA AGGACAAGTC TGGCAGGCTG 
AACCATCAGG AGTTCAAATC TTGCCTGCGC TCCCTGGGCT ATGACCTGCC CATGGTGGAG 
GAAGGGGAAC CTGACCCTGA GTTCGAGGCA ATCCTGGACA CGGTGGATCC GAACAGAGAT 
GGCCATGTCT CCCTGCAAGA ATACATGGCT TTCATGATCA GCCGCGAAAC TGAGAACGTC 
AAGTCCAGCG AGGAGATTGA GAGCGCCTTC CGGGCCCTCA GCTCAGAGGG AAAGCCTTAC 
GTGACCAAGG AGGAGCTCTA CCAGAACCTG ACCCGGGAAC AAGCCGACTA CTGCGTCTCC 
CACATGAAGC CCTACGTGGA CGGCAAGGGC CGCGAGCTCC CCACCGCGTT CGACTACGTG 
GAGTTCACCC GCTCGCTTTT CGTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_016961797.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016817286.2 Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X5, mRNA.

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CloneID	OPc109761
Clone ID Related Accession (Same CDS sequence)	XM_016961797.2
Accession Version	XM_016961797.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7455bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036888.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016961797.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAATTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAATG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA ACACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCCC TGCTAGAGGC AGATGTGGCT 
GCTCATCAGG ACCGAATTGA TGGCATTACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATATTGAGGA TGAGGAGACG TGGATTCGAG AGAAGGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCAGCT GCTTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AGACACGCAT AACTAAGGAG GCCGGCAGTG TATCTCTGCG TATGAAGCAG 
GTGGAAGAAC TATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAGGC TCTAAACACA GACAATTATG GACATGATCT TGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAAT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCGTTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAAATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACGGCAAG TGATGAGTCA TACAAGGATC CCACCAACAT CCAGAGCAAG 
CACCAGAAGC ACCAGGCTTT TGAAGCAGAG CTGCATGCCA ACGCTGACCG GATCCGTGGG 
GTTATCGACA TGGGCAACTC CCTCATTGAA CGTGGAGCCT GTGCCGGCAG TGAGGATGCT 
GTCAAGGCCC GCCTGGCTGC CTTAGCTGAC CAGTGGCAAT TCTTGGTGCA AAAGTCAGCG 
GAAAAGAGCC AGAAACTGAA AGAAGCCAAC AAGCAGCAGA ACTTCAACAC AGGGATCAAG 
GACTTTGACT TCTGGCTGTC TGAGGTGGAG GCCCTGCTGG CATCCGAAGA TTATGGCAAA 
GACCTGGCTT CTGTGAACAA CCTGCTGAAA AAGCATCAAC TGCTGGAAGC AGATATATCT 
GCCCATGAGG ATCGCCTGAA GGACCTGAAC AGCCAGGCAG ACAGCCTGAT GACCAGCAGT 
GCCTTCGACA CCTCCCAAGT AAAGGACAAG AGGGACACCA TCAACGGGCG CTTCCAGAAG 
ATCAAGAGCA TGGCGGCCTC CCGGCGAGCC AAGCTGAATG AATCCCATCG CCTGCACCAG 
TTCTTCCGGG ACATGGATGA CGAGGAGTCC TGGATCAAGG AGAAGAAGCT GCTGGTGGGC 
TCGGAGGACT ACGGCCGGGA CCTAACCGGC GTGCAGAACC TGAGGAAGAA GCACAAGCGC 
CTGGAAGCAG AACTGGCTGC GCATGAGCCG GCTATTCAGG GTGTCCTGGA CACTGGCAAG 
AAGCTGTCCG ATGATAACAC CATCGGGAAA GAGGAGATCC AGCAGCGGCT GGCGCAGTTT 
GTGGAGCACT GGAAAGAGCT GAAGCAGCTG GCAGCTGCCC GGGGTCAGCG GCTGGAAGAG 
TCCTTGGAAT ATCAGCAGTT TGTAGCCAAT GTGGAAGAGG AGGAAGCCTG GATCAATGAG 
AAAATGACCC TGGTGGCCAG CGAAGATTAT GGCGACACTC TTGCTGCCAT CCAGGGCTTA 
CTGAAGAAAC ATGAAGCTTT TGAGACAGAC TTCACCGTCC ACAAGGATCG CGTGAATGAC 
GTCTGCACCA ATGGACAAGA CCTCATTAAG AAGAACAATC ACCATGAGGA GAACATCTCT 
TCAAAGATGA AGGGCCTGAA CGGGAAAGTG TCAGACCTGG AGAAAGCTGC AGCCCAGAGA 
AAGGCGAAGC TGGATGAGAA CTCGGCCTTC CTTCAGTTCA ACTGGAAGGC GGACGTGGTG 
GAGTCCTGGA TCGGTGAAAA GGAGAACAGC TTGAAGACAG ATGATTATGG CCGAGACCTG 
TCTTCTGTGC AGACGCTCCT CACCAAACAG GAAACTTTTG ACGCTGGGCT GCAGGCCTTC 
CAGCAGGAAG GCATTGCCAA CATCACTGCC CTCAAAGATC AGCTTCTCGC CGCCAAACAC 
GTTCAGTCCA AGGCCATCGA GGCCCGGCAC GCCTCCCTCA TGAAGAGGTG GAGCCAGCTT 
CTGGCCAACT CAGCCGCCCG CAAGAAGAAG CTTCTGGAGG CGCAGAGTCA CTTCCGCAAG 
GTGGAGGACC TCTTCCTGAC CTTTGCCAAA AAGGCTTCTG CCTTCAACAG CTGGTTTGAA 
AATGCAGAGG AGGACTTAAC AGACCCCGTG CGCTGCAACT CCTTGGAAGA AATCAAAGCT 
TTGCGCGAGG CCCACGACGC CTTCCGCTCC TCCCTCAGCT CTGCCCAGGC TGACTTCAAC 
CAGCTGGCCG AGCTGGACCG CCAGATCAAG AGCTTCCGCG TAGCCTCCAA CCCCTACACC 
TGGTTTACCA TGGAGGCCCT GGAGGAGACC TGGAGGAACC TACAGAAAAT CATCAAGGAG 
AGGGAGCTGG AGCTGCAGAA GGAACAGCGG CGGCAGGAGG AGAACGACAA GCTGCGCCAG 
GAGTTTGCCC AGCACGCCAA CGCCTTCCAC CAGTGGATCC AAGAGACCAG GACATACCTC 
CTCGATGGGT CCTGTATGGT GGAAGAGTCG GGGACCCTCG AATCCCAGCT TGAAGCTACC 
AAACGCAAGC ACCAGGAAAT CCGAGCCATG AGAAGTCAGC TCAAAAAGAT CGAGGACCTG 
GGGGCCGCCA TGGAGGAGGC CCTCATCCTG GACAACAAGT ACACGGAGCA CAGCACCGTG 
GGCCTCGCCC AGCAGTGGGA CCAGCTGGAC CAGCTGGGCA TGCGCATGCA GCACAACCTG 
GAGCAGCAGA TCCAGGCCAG GAACACAACA GGTGTGACTG AGGAGGCCCT CAAAGAATTC 
AGCATGATGT TTAAACACTT TGACAAGGAC AAGTCTGGCA GGCTGAACCA TCAGGAGTTC 
AAATCTTGCC TGCGCTCCCT GGGCTATGAC CTGCCCATGG TGGAGGAAGG GGAACCTGAC 
CCTGAGTTCG AGGCAATCCT GGACACGGTG GATCCGAACA GAGATGGCCA TGTCTCCCTG 
CAAGAATACA TGGCTTTCAT GATCAGCCGC GAAACTGAGA ACGTCAAGTC CAGCGAGGAG 
ATTGAGAGCG CCTTCCGGGC CCTCAGCTCA GAGGGAAAGC CTTACGTGAC CAAGGAGGAG 
CTCTACCAGA ACCTGACCCG GGAACAAGCC GACTACTGCG TCTCCCACAT GAAGCCCTAC 
GTGGACGGCA AGGGCCGCGA GCTCCCCACC GCGTTCGACT ACGTGGAGTT CACCCGCTCG 
CTTTTCGTGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016817286.2
CDS	121..7575
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDIEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRITKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_016961797.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016961797.2

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAATTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAATG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA ACACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCCC TGCTAGAGGC AGATGTGGCT 
GCTCATCAGG ACCGAATTGA TGGCATTACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATATTGAGGA TGAGGAGACG TGGATTCGAG AGAAGGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCAGCT GCTTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AGACACGCAT AACTAAGGAG GCCGGCAGTG TATCTCTGCG TATGAAGCAG 
GTGGAAGAAC TATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAGGC TCTAAACACA GACAATTATG GACATGATCT TGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAAT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCGTTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAAATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACGGCAAG TGATGAGTCA TACAAGGATC CCACCAACAT CCAGAGCAAG 
CACCAGAAGC ACCAGGCTTT TGAAGCAGAG CTGCATGCCA ACGCTGACCG GATCCGTGGG 
GTTATCGACA TGGGCAACTC CCTCATTGAA CGTGGAGCCT GTGCCGGCAG TGAGGATGCT 
GTCAAGGCCC GCCTGGCTGC CTTAGCTGAC CAGTGGCAAT TCTTGGTGCA AAAGTCAGCG 
GAAAAGAGCC AGAAACTGAA AGAAGCCAAC AAGCAGCAGA ACTTCAACAC AGGGATCAAG 
GACTTTGACT TCTGGCTGTC TGAGGTGGAG GCCCTGCTGG CATCCGAAGA TTATGGCAAA 
GACCTGGCTT CTGTGAACAA CCTGCTGAAA AAGCATCAAC TGCTGGAAGC AGATATATCT 
GCCCATGAGG ATCGCCTGAA GGACCTGAAC AGCCAGGCAG ACAGCCTGAT GACCAGCAGT 
GCCTTCGACA CCTCCCAAGT AAAGGACAAG AGGGACACCA TCAACGGGCG CTTCCAGAAG 
ATCAAGAGCA TGGCGGCCTC CCGGCGAGCC AAGCTGAATG AATCCCATCG CCTGCACCAG 
TTCTTCCGGG ACATGGATGA CGAGGAGTCC TGGATCAAGG AGAAGAAGCT GCTGGTGGGC 
TCGGAGGACT ACGGCCGGGA CCTAACCGGC GTGCAGAACC TGAGGAAGAA GCACAAGCGC 
CTGGAAGCAG AACTGGCTGC GCATGAGCCG GCTATTCAGG GTGTCCTGGA CACTGGCAAG 
AAGCTGTCCG ATGATAACAC CATCGGGAAA GAGGAGATCC AGCAGCGGCT GGCGCAGTTT 
GTGGAGCACT GGAAAGAGCT GAAGCAGCTG GCAGCTGCCC GGGGTCAGCG GCTGGAAGAG 
TCCTTGGAAT ATCAGCAGTT TGTAGCCAAT GTGGAAGAGG AGGAAGCCTG GATCAATGAG 
AAAATGACCC TGGTGGCCAG CGAAGATTAT GGCGACACTC TTGCTGCCAT CCAGGGCTTA 
CTGAAGAAAC ATGAAGCTTT TGAGACAGAC TTCACCGTCC ACAAGGATCG CGTGAATGAC 
GTCTGCACCA ATGGACAAGA CCTCATTAAG AAGAACAATC ACCATGAGGA GAACATCTCT 
TCAAAGATGA AGGGCCTGAA CGGGAAAGTG TCAGACCTGG AGAAAGCTGC AGCCCAGAGA 
AAGGCGAAGC TGGATGAGAA CTCGGCCTTC CTTCAGTTCA ACTGGAAGGC GGACGTGGTG 
GAGTCCTGGA TCGGTGAAAA GGAGAACAGC TTGAAGACAG ATGATTATGG CCGAGACCTG 
TCTTCTGTGC AGACGCTCCT CACCAAACAG GAAACTTTTG ACGCTGGGCT GCAGGCCTTC 
CAGCAGGAAG GCATTGCCAA CATCACTGCC CTCAAAGATC AGCTTCTCGC CGCCAAACAC 
GTTCAGTCCA AGGCCATCGA GGCCCGGCAC GCCTCCCTCA TGAAGAGGTG GAGCCAGCTT 
CTGGCCAACT CAGCCGCCCG CAAGAAGAAG CTTCTGGAGG CGCAGAGTCA CTTCCGCAAG 
GTGGAGGACC TCTTCCTGAC CTTTGCCAAA AAGGCTTCTG CCTTCAACAG CTGGTTTGAA 
AATGCAGAGG AGGACTTAAC AGACCCCGTG CGCTGCAACT CCTTGGAAGA AATCAAAGCT 
TTGCGCGAGG CCCACGACGC CTTCCGCTCC TCCCTCAGCT CTGCCCAGGC TGACTTCAAC 
CAGCTGGCCG AGCTGGACCG CCAGATCAAG AGCTTCCGCG TAGCCTCCAA CCCCTACACC 
TGGTTTACCA TGGAGGCCCT GGAGGAGACC TGGAGGAACC TACAGAAAAT CATCAAGGAG 
AGGGAGCTGG AGCTGCAGAA GGAACAGCGG CGGCAGGAGG AGAACGACAA GCTGCGCCAG 
GAGTTTGCCC AGCACGCCAA CGCCTTCCAC CAGTGGATCC AAGAGACCAG GACATACCTC 
CTCGATGGGT CCTGTATGGT GGAAGAGTCG GGGACCCTCG AATCCCAGCT TGAAGCTACC 
AAACGCAAGC ACCAGGAAAT CCGAGCCATG AGAAGTCAGC TCAAAAAGAT CGAGGACCTG 
GGGGCCGCCA TGGAGGAGGC CCTCATCCTG GACAACAAGT ACACGGAGCA CAGCACCGTG 
GGCCTCGCCC AGCAGTGGGA CCAGCTGGAC CAGCTGGGCA TGCGCATGCA GCACAACCTG 
GAGCAGCAGA TCCAGGCCAG GAACACAACA GGTGTGACTG AGGAGGCCCT CAAAGAATTC 
AGCATGATGT TTAAACACTT TGACAAGGAC AAGTCTGGCA GGCTGAACCA TCAGGAGTTC 
AAATCTTGCC TGCGCTCCCT GGGCTATGAC CTGCCCATGG TGGAGGAAGG GGAACCTGAC 
CCTGAGTTCG AGGCAATCCT GGACACGGTG GATCCGAACA GAGATGGCCA TGTCTCCCTG 
CAAGAATACA TGGCTTTCAT GATCAGCCGC GAAACTGAGA ACGTCAAGTC CAGCGAGGAG 
ATTGAGAGCG CCTTCCGGGC CCTCAGCTCA GAGGGAAAGC CTTACGTGAC CAAGGAGGAG 
CTCTACCAGA ACCTGACCCG GGAACAAGCC GACTACTGCG TCTCCCACAT GAAGCCCTAC 
GTGGACGGCA AGGGCCGCGA GCTCCCCACC GCGTTCGACT ACGTGGAGTT CACCCGCTCG 
CTTTTCGTGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_009457423.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009455698.3 Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X6, mRNA.

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CloneID	OPc109762
Clone ID Related Accession (Same CDS sequence)	XM_009457423.3
Accession Version	XM_009457423.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7452bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036888.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009457423.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAATTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAATG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA ACACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCCC TGCTAGAGGC AGATGTGGCT 
GCTCATCAGG ACCGAATTGA TGGCATTACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATATTGAGGA TGAGGAGACG TGGATTCGAG AGAAGGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCAGCT GCTTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AGACACGCAT AACTAAGGAG GCCGGCAGTG TATCTCTGCG TATGAAGCAG 
GTGGAAGAAC TATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAGGC TCTAAACACA GACAATTATG GACATGATCT TGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAAT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCGTTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAAATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACGGCAAG TGATGAGTCA TACAAGGATC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCACCA GAAGCACCAG GCTTTTGAAG CAGAGCTGCA TGCCAACGCT 
GACCGGATCC GTGGGGTTAT CGACATGGGC AACTCCCTCA TTGAACGTGG AGCCTGTGCC 
GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCTTAG CTGACCAGTG GCAATTCTTG 
GTGCAAAAGT CAGCGGAAAA GAGCCAGAAA CTGAAAGAAG CCAACAAGCA GCAGAACTTC 
AACACAGGGA TCAAGGACTT TGACTTCTGG CTGTCTGAGG TGGAGGCCCT GCTGGCATCC 
GAAGATTATG GCAAAGACCT GGCTTCTGTG AACAACCTGC TGAAAAAGCA TCAACTGCTG 
GAAGCAGATA TATCTGCCCA TGAGGATCGC CTGAAGGACC TGAACAGCCA GGCAGACAGC 
CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTAAAGG ACAAGAGGGA CACCATCAAC 
GGGCGCTTCC AGAAGATCAA GAGCATGGCG GCCTCCCGGC GAGCCAAGCT GAATGAATCC 
CATCGCCTGC ACCAGTTCTT CCGGGACATG GATGACGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGGGCTCGGA GGACTACGGC CGGGACCTAA CCGGCGTGCA GAACCTGAGG 
AAGAAGCACA AGCGCCTGGA AGCAGAACTG GCTGCGCATG AGCCGGCTAT TCAGGGTGTC 
CTGGACACTG GCAAGAAGCT GTCCGATGAT AACACCATCG GGAAAGAGGA GATCCAGCAG 
CGGCTGGCGC AGTTTGTGGA GCACTGGAAA GAGCTGAAGC AGCTGGCAGC TGCCCGGGGT 
CAGCGGCTGG AAGAGTCCTT GGAATATCAG CAGTTTGTAG CCAATGTGGA AGAGGAGGAA 
GCCTGGATCA ATGAGAAAAT GACCCTGGTG GCCAGCGAAG ATTATGGCGA CACTCTTGCT 
GCCATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC CGTCCACAAG 
GATCGCGTGA ATGACGTCTG CACCAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCAAA GATGAAGGGC CTGAACGGGA AAGTGTCAGA CCTGGAGAAA 
GCTGCAGCCC AGAGAAAGGC GAAGCTGGAT GAGAACTCGG CCTTCCTTCA GTTCAACTGG 
AAGGCGGACG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGCCGAG ACCTGTCTTC TGTGCAGACG CTCCTCACCA AACAGGAAAC TTTTGACGCT 
GGGCTGCAGG CCTTCCAGCA GGAAGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTT 
CTCGCCGCCA AACACGTTCA GTCCAAGGCC ATCGAGGCCC GGCACGCCTC CCTCATGAAG 
AGGTGGAGCC AGCTTCTGGC CAACTCAGCC GCCCGCAAGA AGAAGCTTCT GGAGGCGCAG 
AGTCACTTCC GCAAGGTGGA GGACCTCTTC CTGACCTTTG CCAAAAAGGC TTCTGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CCGTGCGCTG CAACTCCTTG 
GAAGAAATCA AAGCTTTGCG CGAGGCCCAC GACGCCTTCC GCTCCTCCCT CAGCTCTGCC 
CAGGCTGACT TCAACCAGCT GGCCGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTAGCC 
TCCAACCCCT ACACCTGGTT TACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTACAG 
AAAATCATCA AGGAGAGGGA GCTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT TGCCCAGCAC GCCAACGCCT TCCACCAGTG GATCCAAGAG 
ACCAGGTCCT GTATGGTGGA AGAGTCGGGG ACCCTCGAAT CCCAGCTTGA AGCTACCAAA 
CGCAAGCACC AGGAAATCCG AGCCATGAGA AGTCAGCTCA AAAAGATCGA GGACCTGGGG 
GCCGCCATGG AGGAGGCCCT CATCCTGGAC AACAAGTACA CGGAGCACAG CACCGTGGGC 
CTCGCCCAGC AGTGGGACCA GCTGGACCAG CTGGGCATGC GCATGCAGCA CAACCTGGAG 
CAGCAGATCC AGGCCAGGAA CACAACAGGT GTGACTGAGG AGGCCCTCAA AGAATTCAGC 
ATGATGTTTA AACACTTTGA CAAGGACAAG TCTGGCAGGC TGAACCATCA GGAGTTCAAA 
TCTTGCCTGC GCTCCCTGGG CTATGACCTG CCCATGGTGG AGGAAGGGGA ACCTGACCCT 
GAGTTCGAGG CAATCCTGGA CACGGTGGAT CCGAACAGAG ATGGCCATGT CTCCCTGCAA 
GAATACATGG CTTTCATGAT CAGCCGCGAA ACTGAGAACG TCAAGTCCAG CGAGGAGATT 
GAGAGCGCCT TCCGGGCCCT CAGCTCAGAG GGAAAGCCTT ACGTGACCAA GGAGGAGCTC 
TACCAGAACC TGACCCGGGA ACAAGCCGAC TACTGCGTCT CCCACATGAA GCCCTACGTG 
GACGGCAAGG GCCGCGAGCT CCCCACCGCG TTCGACTACG TGGAGTTCAC CCGCTCGCTT 
TTCGTGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009455698.3
CDS	121..7572
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDIEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRITKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_009457423.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009457423.3

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAATTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAATG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA ACACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCCC TGCTAGAGGC AGATGTGGCT 
GCTCATCAGG ACCGAATTGA TGGCATTACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATATTGAGGA TGAGGAGACG TGGATTCGAG AGAAGGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCAGCT GCTTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AGACACGCAT AACTAAGGAG GCCGGCAGTG TATCTCTGCG TATGAAGCAG 
GTGGAAGAAC TATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAGGC TCTAAACACA GACAATTATG GACATGATCT TGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAAT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCGTTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAAATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACGGCAAG TGATGAGTCA TACAAGGATC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCACCA GAAGCACCAG GCTTTTGAAG CAGAGCTGCA TGCCAACGCT 
GACCGGATCC GTGGGGTTAT CGACATGGGC AACTCCCTCA TTGAACGTGG AGCCTGTGCC 
GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCTTAG CTGACCAGTG GCAATTCTTG 
GTGCAAAAGT CAGCGGAAAA GAGCCAGAAA CTGAAAGAAG CCAACAAGCA GCAGAACTTC 
AACACAGGGA TCAAGGACTT TGACTTCTGG CTGTCTGAGG TGGAGGCCCT GCTGGCATCC 
GAAGATTATG GCAAAGACCT GGCTTCTGTG AACAACCTGC TGAAAAAGCA TCAACTGCTG 
GAAGCAGATA TATCTGCCCA TGAGGATCGC CTGAAGGACC TGAACAGCCA GGCAGACAGC 
CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTAAAGG ACAAGAGGGA CACCATCAAC 
GGGCGCTTCC AGAAGATCAA GAGCATGGCG GCCTCCCGGC GAGCCAAGCT GAATGAATCC 
CATCGCCTGC ACCAGTTCTT CCGGGACATG GATGACGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGGGCTCGGA GGACTACGGC CGGGACCTAA CCGGCGTGCA GAACCTGAGG 
AAGAAGCACA AGCGCCTGGA AGCAGAACTG GCTGCGCATG AGCCGGCTAT TCAGGGTGTC 
CTGGACACTG GCAAGAAGCT GTCCGATGAT AACACCATCG GGAAAGAGGA GATCCAGCAG 
CGGCTGGCGC AGTTTGTGGA GCACTGGAAA GAGCTGAAGC AGCTGGCAGC TGCCCGGGGT 
CAGCGGCTGG AAGAGTCCTT GGAATATCAG CAGTTTGTAG CCAATGTGGA AGAGGAGGAA 
GCCTGGATCA ATGAGAAAAT GACCCTGGTG GCCAGCGAAG ATTATGGCGA CACTCTTGCT 
GCCATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC CGTCCACAAG 
GATCGCGTGA ATGACGTCTG CACCAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCAAA GATGAAGGGC CTGAACGGGA AAGTGTCAGA CCTGGAGAAA 
GCTGCAGCCC AGAGAAAGGC GAAGCTGGAT GAGAACTCGG CCTTCCTTCA GTTCAACTGG 
AAGGCGGACG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGCCGAG ACCTGTCTTC TGTGCAGACG CTCCTCACCA AACAGGAAAC TTTTGACGCT 
GGGCTGCAGG CCTTCCAGCA GGAAGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTT 
CTCGCCGCCA AACACGTTCA GTCCAAGGCC ATCGAGGCCC GGCACGCCTC CCTCATGAAG 
AGGTGGAGCC AGCTTCTGGC CAACTCAGCC GCCCGCAAGA AGAAGCTTCT GGAGGCGCAG 
AGTCACTTCC GCAAGGTGGA GGACCTCTTC CTGACCTTTG CCAAAAAGGC TTCTGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CCGTGCGCTG CAACTCCTTG 
GAAGAAATCA AAGCTTTGCG CGAGGCCCAC GACGCCTTCC GCTCCTCCCT CAGCTCTGCC 
CAGGCTGACT TCAACCAGCT GGCCGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTAGCC 
TCCAACCCCT ACACCTGGTT TACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTACAG 
AAAATCATCA AGGAGAGGGA GCTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT TGCCCAGCAC GCCAACGCCT TCCACCAGTG GATCCAAGAG 
ACCAGGTCCT GTATGGTGGA AGAGTCGGGG ACCCTCGAAT CCCAGCTTGA AGCTACCAAA 
CGCAAGCACC AGGAAATCCG AGCCATGAGA AGTCAGCTCA AAAAGATCGA GGACCTGGGG 
GCCGCCATGG AGGAGGCCCT CATCCTGGAC AACAAGTACA CGGAGCACAG CACCGTGGGC 
CTCGCCCAGC AGTGGGACCA GCTGGACCAG CTGGGCATGC GCATGCAGCA CAACCTGGAG 
CAGCAGATCC AGGCCAGGAA CACAACAGGT GTGACTGAGG AGGCCCTCAA AGAATTCAGC 
ATGATGTTTA AACACTTTGA CAAGGACAAG TCTGGCAGGC TGAACCATCA GGAGTTCAAA 
TCTTGCCTGC GCTCCCTGGG CTATGACCTG CCCATGGTGG AGGAAGGGGA ACCTGACCCT 
GAGTTCGAGG CAATCCTGGA CACGGTGGAT CCGAACAGAG ATGGCCATGT CTCCCTGCAA 
GAATACATGG CTTTCATGAT CAGCCGCGAA ACTGAGAACG TCAAGTCCAG CGAGGAGATT 
GAGAGCGCCT TCCGGGCCCT CAGCTCAGAG GGAAAGCCTT ACGTGACCAA GGAGGAGCTC 
TACCAGAACC TGACCCGGGA ACAAGCCGAC TACTGCGTCT CCCACATGAA GCCCTACGTG 
GACGGCAAGG GCCGCGAGCT CCCCACCGCG TTCGACTACG TGGAGTTCAC CCGCTCGCTT 
TTCGTGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_024345907.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_024201675.1 Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X8, mRNA.

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CloneID	OPc109763
Clone ID Related Accession (Same CDS sequence)	XM_024345907.1 , XM_009457424.3
Accession Version	XM_024345907.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7437bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036888.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAA TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AATGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAACACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCCCTGCTAG AGGCAGATGT GGCTGCTCAT CAGGACCGAA TTGATGGCAT TACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATATTG AGGATGAGGA GACGTGGATT 
CGAGAGAAGG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC AGCTGCTTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AGGCTCTAAA CACAGACAAT 
TATGGACATG ATCTTGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAATCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCGTT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAAATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACGG CAAGTGATGA GTCATACAAG 
GATCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAGGCTTTT 
GAAGCAGAGC TGCATGCCAA CGCTGACCGG ATCCGTGGGG TTATCGACAT GGGCAACTCC 
CTCATTGAAC GTGGAGCCTG TGCCGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
TTAGCTGACC AGTGGCAATT CTTGGTGCAA AAGTCAGCGG AAAAGAGCCA GAAACTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACA GGGATCAAGG ACTTTGACTT CTGGCTGTCT 
GAGGTGGAGG CCCTGCTGGC ATCCGAAGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC 
CTGCTGAAAA AGCATCAACT GCTGGAAGCA GATATATCTG CCCATGAGGA TCGCCTGAAG 
GACCTGAACA GCCAGGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTA 
AAGGACAAGA GGGACACCAT CAACGGGCGC TTCCAGAAGA TCAAGAGCAT GGCGGCCTCC 
CGGCGAGCCA AGCTGAATGA ATCCCATCGC CTGCACCAGT TCTTCCGGGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGGGCT CGGAGGACTA CGGCCGGGAC 
CTAACCGGCG TGCAGAACCT GAGGAAGAAG CACAAGCGCC TGGAAGCAGA ACTGGCTGCG 
CATGAGCCGG CTATTCAGGG TGTCCTGGAC ACTGGCAAGA AGCTGTCCGA TGATAACACC 
ATCGGGAAAG AGGAGATCCA GCAGCGGCTG GCGCAGTTTG TGGAGCACTG GAAAGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGTCAGCGG CTGGAAGAGT CCTTGGAATA TCAGCAGTTT 
GTAGCCAATG TGGAAGAGGA GGAAGCCTGG ATCAATGAGA AAATGACCCT GGTGGCCAGC 
GAAGATTATG GCGACACTCT TGCTGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACCGTCCA CAAGGATCGC GTGAATGACG TCTGCACCAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGCCTGAAC 
GGGAAAGTGT CAGACCTGGA GAAAGCTGCA GCCCAGAGAA AGGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA CTGGAAGGCG GACGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGC CGAGACCTGT CTTCTGTGCA GACGCTCCTC 
ACCAAACAGG AAACTTTTGA CGCTGGGCTG CAGGCCTTCC AGCAGGAAGG CATTGCCAAC 
ATCACTGCCC TCAAAGATCA GCTTCTCGCC GCCAAACACG TTCAGTCCAA GGCCATCGAG 
GCCCGGCACG CCTCCCTCAT GAAGAGGTGG AGCCAGCTTC TGGCCAACTC AGCCGCCCGC 
AAGAAGAAGC TTCTGGAGGC GCAGAGTCAC TTCCGCAAGG TGGAGGACCT CTTCCTGACC 
TTTGCCAAAA AGGCTTCTGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCCGTGC GCTGCAACTC CTTGGAAGAA ATCAAAGCTT TGCGCGAGGC CCACGACGCC 
TTCCGCTCCT CCCTCAGCTC TGCCCAGGCT GACTTCAACC AGCTGGCCGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT AGCCTCCAAC CCCTACACCT GGTTTACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT ACAGAAAATC ATCAAGGAGA GGGAGCTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTTGCCCA GCACGCCAAC 
GCCTTCCACC AGTGGATCCA AGAGACCAGG ACATACCTCC TCGATGGCAT AGCATATCGT 
CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGTATG 
GTGGAAGAGT CGGGGACCCT CGAATCCCAG CTTGAAGCTA CCAAACGCAA GCACCAGGAA 
ATCCGAGCCA TGAGAAGTCA GCTCAAAAAG ATCGAGGACC TGGGGGCCGC CATGGAGGAG 
GCCCTCATCC TGGACAACAA GTACACGGAG CACAGCACCG TGGGCCTCGC CCAGCAGTGG 
GACCAGCTGG ACCAGCTGGG CATGCGCATG CAGCACAACC TGGAGCAGCA GATCCAGGCC 
AGGAACACAA CAGGTGTGAC TGAGGAGGCC CTCAAAGAAT TCAGCATGAT GTTTAAACAC 
TTTGACAAGG ACAAGTCTGG CAGGCTGAAC CATCAGGAGT TCAAATCTTG CCTGCGCTCC 
CTGGGCTATG ACCTGCCCAT GGTGGAGGAA GGGGAACCTG ACCCTGAGTT CGAGGCAATC 
CTGGACACGG TGGATCCGAA CAGAGATGGC CATGTCTCCC TGCAAGAATA CATGGCTTTC 
ATGATCAGCC GCGAAACTGA GAACGTCAAG TCCAGCGAGG AGATTGAGAG CGCCTTCCGG 
GCCCTCAGCT CAGAGGGAAA GCCTTACGTG ACCAAGGAGG AGCTCTACCA GAACCTGACC 
CGGGAACAAG CCGACTACTG CGTCTCCCAC ATGAAGCCCT ACGTGGACGG CAAGGGCCGC 
GAGCTCCCCA CCGCGTTCGA CTACGTGGAG TTCACCCGCT CGCTTTTCGT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024201675.1
CDS	278..7714
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDIEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_024345907.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024345907.1

ORF Insert Sequence:

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAA TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AATGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAACACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCCCTGCTAG AGGCAGATGT GGCTGCTCAT CAGGACCGAA TTGATGGCAT TACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATATTG AGGATGAGGA GACGTGGATT 
CGAGAGAAGG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC AGCTGCTTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AGGCTCTAAA CACAGACAAT 
TATGGACATG ATCTTGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAATCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCGTT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAAATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACGG CAAGTGATGA GTCATACAAG 
GATCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAGGCTTTT 
GAAGCAGAGC TGCATGCCAA CGCTGACCGG ATCCGTGGGG TTATCGACAT GGGCAACTCC 
CTCATTGAAC GTGGAGCCTG TGCCGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
TTAGCTGACC AGTGGCAATT CTTGGTGCAA AAGTCAGCGG AAAAGAGCCA GAAACTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACA GGGATCAAGG ACTTTGACTT CTGGCTGTCT 
GAGGTGGAGG CCCTGCTGGC ATCCGAAGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC 
CTGCTGAAAA AGCATCAACT GCTGGAAGCA GATATATCTG CCCATGAGGA TCGCCTGAAG 
GACCTGAACA GCCAGGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTA 
AAGGACAAGA GGGACACCAT CAACGGGCGC TTCCAGAAGA TCAAGAGCAT GGCGGCCTCC 
CGGCGAGCCA AGCTGAATGA ATCCCATCGC CTGCACCAGT TCTTCCGGGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGGGCT CGGAGGACTA CGGCCGGGAC 
CTAACCGGCG TGCAGAACCT GAGGAAGAAG CACAAGCGCC TGGAAGCAGA ACTGGCTGCG 
CATGAGCCGG CTATTCAGGG TGTCCTGGAC ACTGGCAAGA AGCTGTCCGA TGATAACACC 
ATCGGGAAAG AGGAGATCCA GCAGCGGCTG GCGCAGTTTG TGGAGCACTG GAAAGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGTCAGCGG CTGGAAGAGT CCTTGGAATA TCAGCAGTTT 
GTAGCCAATG TGGAAGAGGA GGAAGCCTGG ATCAATGAGA AAATGACCCT GGTGGCCAGC 
GAAGATTATG GCGACACTCT TGCTGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACCGTCCA CAAGGATCGC GTGAATGACG TCTGCACCAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGCCTGAAC 
GGGAAAGTGT CAGACCTGGA GAAAGCTGCA GCCCAGAGAA AGGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA CTGGAAGGCG GACGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGC CGAGACCTGT CTTCTGTGCA GACGCTCCTC 
ACCAAACAGG AAACTTTTGA CGCTGGGCTG CAGGCCTTCC AGCAGGAAGG CATTGCCAAC 
ATCACTGCCC TCAAAGATCA GCTTCTCGCC GCCAAACACG TTCAGTCCAA GGCCATCGAG 
GCCCGGCACG CCTCCCTCAT GAAGAGGTGG AGCCAGCTTC TGGCCAACTC AGCCGCCCGC 
AAGAAGAAGC TTCTGGAGGC GCAGAGTCAC TTCCGCAAGG TGGAGGACCT CTTCCTGACC 
TTTGCCAAAA AGGCTTCTGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCCGTGC GCTGCAACTC CTTGGAAGAA ATCAAAGCTT TGCGCGAGGC CCACGACGCC 
TTCCGCTCCT CCCTCAGCTC TGCCCAGGCT GACTTCAACC AGCTGGCCGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT AGCCTCCAAC CCCTACACCT GGTTTACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT ACAGAAAATC ATCAAGGAGA GGGAGCTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTTGCCCA GCACGCCAAC 
GCCTTCCACC AGTGGATCCA AGAGACCAGG ACATACCTCC TCGATGGCAT AGCATATCGT 
CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGTATG 
GTGGAAGAGT CGGGGACCCT CGAATCCCAG CTTGAAGCTA CCAAACGCAA GCACCAGGAA 
ATCCGAGCCA TGAGAAGTCA GCTCAAAAAG ATCGAGGACC TGGGGGCCGC CATGGAGGAG 
GCCCTCATCC TGGACAACAA GTACACGGAG CACAGCACCG TGGGCCTCGC CCAGCAGTGG 
GACCAGCTGG ACCAGCTGGG CATGCGCATG CAGCACAACC TGGAGCAGCA GATCCAGGCC 
AGGAACACAA CAGGTGTGAC TGAGGAGGCC CTCAAAGAAT TCAGCATGAT GTTTAAACAC 
TTTGACAAGG ACAAGTCTGG CAGGCTGAAC CATCAGGAGT TCAAATCTTG CCTGCGCTCC 
CTGGGCTATG ACCTGCCCAT GGTGGAGGAA GGGGAACCTG ACCCTGAGTT CGAGGCAATC 
CTGGACACGG TGGATCCGAA CAGAGATGGC CATGTCTCCC TGCAAGAATA CATGGCTTTC 
ATGATCAGCC GCGAAACTGA GAACGTCAAG TCCAGCGAGG AGATTGAGAG CGCCTTCCGG 
GCCCTCAGCT CAGAGGGAAA GCCTTACGTG ACCAAGGAGG AGCTCTACCA GAACCTGACC 
CGGGAACAAG CCGACTACTG CGTCTCCCAC ATGAAGCCCT ACGTGGACGG CAAGGGCCGC 
GAGCTCCCCA CCGCGTTCGA CTACGTGGAG TTCACCCGCT CGCTTTTCGT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_009457424.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009455699.3 Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X7, mRNA.

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CloneID	OPc109763
Clone ID Related Accession (Same CDS sequence)	XM_024345907.1 , XM_009457424.3
Accession Version	XM_009457424.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7437bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036888.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009457424.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAA TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AATGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAACACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCCCTGCTAG AGGCAGATGT GGCTGCTCAT CAGGACCGAA TTGATGGCAT TACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATATTG AGGATGAGGA GACGTGGATT 
CGAGAGAAGG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC AGCTGCTTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AGGCTCTAAA CACAGACAAT 
TATGGACATG ATCTTGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAATCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCGTT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAAATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACGG CAAGTGATGA GTCATACAAG 
GATCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAGGCTTTT 
GAAGCAGAGC TGCATGCCAA CGCTGACCGG ATCCGTGGGG TTATCGACAT GGGCAACTCC 
CTCATTGAAC GTGGAGCCTG TGCCGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
TTAGCTGACC AGTGGCAATT CTTGGTGCAA AAGTCAGCGG AAAAGAGCCA GAAACTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACA GGGATCAAGG ACTTTGACTT CTGGCTGTCT 
GAGGTGGAGG CCCTGCTGGC ATCCGAAGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC 
CTGCTGAAAA AGCATCAACT GCTGGAAGCA GATATATCTG CCCATGAGGA TCGCCTGAAG 
GACCTGAACA GCCAGGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTA 
AAGGACAAGA GGGACACCAT CAACGGGCGC TTCCAGAAGA TCAAGAGCAT GGCGGCCTCC 
CGGCGAGCCA AGCTGAATGA ATCCCATCGC CTGCACCAGT TCTTCCGGGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGGGCT CGGAGGACTA CGGCCGGGAC 
CTAACCGGCG TGCAGAACCT GAGGAAGAAG CACAAGCGCC TGGAAGCAGA ACTGGCTGCG 
CATGAGCCGG CTATTCAGGG TGTCCTGGAC ACTGGCAAGA AGCTGTCCGA TGATAACACC 
ATCGGGAAAG AGGAGATCCA GCAGCGGCTG GCGCAGTTTG TGGAGCACTG GAAAGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGTCAGCGG CTGGAAGAGT CCTTGGAATA TCAGCAGTTT 
GTAGCCAATG TGGAAGAGGA GGAAGCCTGG ATCAATGAGA AAATGACCCT GGTGGCCAGC 
GAAGATTATG GCGACACTCT TGCTGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACCGTCCA CAAGGATCGC GTGAATGACG TCTGCACCAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGCCTGAAC 
GGGAAAGTGT CAGACCTGGA GAAAGCTGCA GCCCAGAGAA AGGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA CTGGAAGGCG GACGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGC CGAGACCTGT CTTCTGTGCA GACGCTCCTC 
ACCAAACAGG AAACTTTTGA CGCTGGGCTG CAGGCCTTCC AGCAGGAAGG CATTGCCAAC 
ATCACTGCCC TCAAAGATCA GCTTCTCGCC GCCAAACACG TTCAGTCCAA GGCCATCGAG 
GCCCGGCACG CCTCCCTCAT GAAGAGGTGG AGCCAGCTTC TGGCCAACTC AGCCGCCCGC 
AAGAAGAAGC TTCTGGAGGC GCAGAGTCAC TTCCGCAAGG TGGAGGACCT CTTCCTGACC 
TTTGCCAAAA AGGCTTCTGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCCGTGC GCTGCAACTC CTTGGAAGAA ATCAAAGCTT TGCGCGAGGC CCACGACGCC 
TTCCGCTCCT CCCTCAGCTC TGCCCAGGCT GACTTCAACC AGCTGGCCGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT AGCCTCCAAC CCCTACACCT GGTTTACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT ACAGAAAATC ATCAAGGAGA GGGAGCTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTTGCCCA GCACGCCAAC 
GCCTTCCACC AGTGGATCCA AGAGACCAGG ACATACCTCC TCGATGGCAT AGCATATCGT 
CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGTATG 
GTGGAAGAGT CGGGGACCCT CGAATCCCAG CTTGAAGCTA CCAAACGCAA GCACCAGGAA 
ATCCGAGCCA TGAGAAGTCA GCTCAAAAAG ATCGAGGACC TGGGGGCCGC CATGGAGGAG 
GCCCTCATCC TGGACAACAA GTACACGGAG CACAGCACCG TGGGCCTCGC CCAGCAGTGG 
GACCAGCTGG ACCAGCTGGG CATGCGCATG CAGCACAACC TGGAGCAGCA GATCCAGGCC 
AGGAACACAA CAGGTGTGAC TGAGGAGGCC CTCAAAGAAT TCAGCATGAT GTTTAAACAC 
TTTGACAAGG ACAAGTCTGG CAGGCTGAAC CATCAGGAGT TCAAATCTTG CCTGCGCTCC 
CTGGGCTATG ACCTGCCCAT GGTGGAGGAA GGGGAACCTG ACCCTGAGTT CGAGGCAATC 
CTGGACACGG TGGATCCGAA CAGAGATGGC CATGTCTCCC TGCAAGAATA CATGGCTTTC 
ATGATCAGCC GCGAAACTGA GAACGTCAAG TCCAGCGAGG AGATTGAGAG CGCCTTCCGG 
GCCCTCAGCT CAGAGGGAAA GCCTTACGTG ACCAAGGAGG AGCTCTACCA GAACCTGACC 
CGGGAACAAG CCGACTACTG CGTCTCCCAC ATGAAGCCCT ACGTGGACGG CAAGGGCCGC 
GAGCTCCCCA CCGCGTTCGA CTACGTGGAG TTCACCCGCT CGCTTTTCGT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009455699.3
CDS	108..7544
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDIEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_009457424.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009457424.3

ORF Insert Sequence:

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAA TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AATGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAACACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCCCTGCTAG AGGCAGATGT GGCTGCTCAT CAGGACCGAA TTGATGGCAT TACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATATTG AGGATGAGGA GACGTGGATT 
CGAGAGAAGG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC AGCTGCTTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AGGCTCTAAA CACAGACAAT 
TATGGACATG ATCTTGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAATCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCGTT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAAATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACGG CAAGTGATGA GTCATACAAG 
GATCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAGGCTTTT 
GAAGCAGAGC TGCATGCCAA CGCTGACCGG ATCCGTGGGG TTATCGACAT GGGCAACTCC 
CTCATTGAAC GTGGAGCCTG TGCCGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
TTAGCTGACC AGTGGCAATT CTTGGTGCAA AAGTCAGCGG AAAAGAGCCA GAAACTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACA GGGATCAAGG ACTTTGACTT CTGGCTGTCT 
GAGGTGGAGG CCCTGCTGGC ATCCGAAGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC 
CTGCTGAAAA AGCATCAACT GCTGGAAGCA GATATATCTG CCCATGAGGA TCGCCTGAAG 
GACCTGAACA GCCAGGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTA 
AAGGACAAGA GGGACACCAT CAACGGGCGC TTCCAGAAGA TCAAGAGCAT GGCGGCCTCC 
CGGCGAGCCA AGCTGAATGA ATCCCATCGC CTGCACCAGT TCTTCCGGGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGGGCT CGGAGGACTA CGGCCGGGAC 
CTAACCGGCG TGCAGAACCT GAGGAAGAAG CACAAGCGCC TGGAAGCAGA ACTGGCTGCG 
CATGAGCCGG CTATTCAGGG TGTCCTGGAC ACTGGCAAGA AGCTGTCCGA TGATAACACC 
ATCGGGAAAG AGGAGATCCA GCAGCGGCTG GCGCAGTTTG TGGAGCACTG GAAAGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGTCAGCGG CTGGAAGAGT CCTTGGAATA TCAGCAGTTT 
GTAGCCAATG TGGAAGAGGA GGAAGCCTGG ATCAATGAGA AAATGACCCT GGTGGCCAGC 
GAAGATTATG GCGACACTCT TGCTGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACCGTCCA CAAGGATCGC GTGAATGACG TCTGCACCAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGCCTGAAC 
GGGAAAGTGT CAGACCTGGA GAAAGCTGCA GCCCAGAGAA AGGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA CTGGAAGGCG GACGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGC CGAGACCTGT CTTCTGTGCA GACGCTCCTC 
ACCAAACAGG AAACTTTTGA CGCTGGGCTG CAGGCCTTCC AGCAGGAAGG CATTGCCAAC 
ATCACTGCCC TCAAAGATCA GCTTCTCGCC GCCAAACACG TTCAGTCCAA GGCCATCGAG 
GCCCGGCACG CCTCCCTCAT GAAGAGGTGG AGCCAGCTTC TGGCCAACTC AGCCGCCCGC 
AAGAAGAAGC TTCTGGAGGC GCAGAGTCAC TTCCGCAAGG TGGAGGACCT CTTCCTGACC 
TTTGCCAAAA AGGCTTCTGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCCGTGC GCTGCAACTC CTTGGAAGAA ATCAAAGCTT TGCGCGAGGC CCACGACGCC 
TTCCGCTCCT CCCTCAGCTC TGCCCAGGCT GACTTCAACC AGCTGGCCGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT AGCCTCCAAC CCCTACACCT GGTTTACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT ACAGAAAATC ATCAAGGAGA GGGAGCTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTTGCCCA GCACGCCAAC 
GCCTTCCACC AGTGGATCCA AGAGACCAGG ACATACCTCC TCGATGGCAT AGCATATCGT 
CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGTATG 
GTGGAAGAGT CGGGGACCCT CGAATCCCAG CTTGAAGCTA CCAAACGCAA GCACCAGGAA 
ATCCGAGCCA TGAGAAGTCA GCTCAAAAAG ATCGAGGACC TGGGGGCCGC CATGGAGGAG 
GCCCTCATCC TGGACAACAA GTACACGGAG CACAGCACCG TGGGCCTCGC CCAGCAGTGG 
GACCAGCTGG ACCAGCTGGG CATGCGCATG CAGCACAACC TGGAGCAGCA GATCCAGGCC 
AGGAACACAA CAGGTGTGAC TGAGGAGGCC CTCAAAGAAT TCAGCATGAT GTTTAAACAC 
TTTGACAAGG ACAAGTCTGG CAGGCTGAAC CATCAGGAGT TCAAATCTTG CCTGCGCTCC 
CTGGGCTATG ACCTGCCCAT GGTGGAGGAA GGGGAACCTG ACCCTGAGTT CGAGGCAATC 
CTGGACACGG TGGATCCGAA CAGAGATGGC CATGTCTCCC TGCAAGAATA CATGGCTTTC 
ATGATCAGCC GCGAAACTGA GAACGTCAAG TCCAGCGAGG AGATTGAGAG CGCCTTCCGG 
GCCCTCAGCT CAGAGGGAAA GCCTTACGTG ACCAAGGAGG AGCTCTACCA GAACCTGACC 
CGGGAACAAG CCGACTACTG CGTCTCCCAC ATGAAGCCCT ACGTGGACGG CAAGGGCCGC 
GAGCTCCCCA CCGCGTTCGA CTACGTGGAG TTCACCCGCT CGCTTTTCGT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_009457425.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009455700.3 Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X9, mRNA.

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CloneID	OPc109764
Clone ID Related Accession (Same CDS sequence)	XM_009457425.3
Accession Version	XM_009457425.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7434bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036888.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009457425.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAA TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AATGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAACACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCCCTGCTAG AGGCAGATGT GGCTGCTCAT CAGGACCGAA TTGATGGCAT TACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATATTG AGGATGAGGA GACGTGGATT 
CGAGAGAAGG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC AGCTGCTTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAGACAC GCATAACTAA GGAGGCCGGC 
AGTGTATCTC TGCGTATGAA GCAGGTGGAA GAACTATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AGGCTCTAAA CACAGACAAT 
TATGGACATG ATCTTGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAATCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCGTT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAAATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACGG CAAGTGATGA GTCATACAAG 
GATCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAGGCTTTT 
GAAGCAGAGC TGCATGCCAA CGCTGACCGG ATCCGTGGGG TTATCGACAT GGGCAACTCC 
CTCATTGAAC GTGGAGCCTG TGCCGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
TTAGCTGACC AGTGGCAATT CTTGGTGCAA AAGTCAGCGG AAAAGAGCCA GAAACTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACA GGGATCAAGG ACTTTGACTT CTGGCTGTCT 
GAGGTGGAGG CCCTGCTGGC ATCCGAAGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC 
CTGCTGAAAA AGCATCAACT GCTGGAAGCA GATATATCTG CCCATGAGGA TCGCCTGAAG 
GACCTGAACA GCCAGGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTA 
AAGGACAAGA GGGACACCAT CAACGGGCGC TTCCAGAAGA TCAAGAGCAT GGCGGCCTCC 
CGGCGAGCCA AGCTGAATGA ATCCCATCGC CTGCACCAGT TCTTCCGGGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGGGCT CGGAGGACTA CGGCCGGGAC 
CTAACCGGCG TGCAGAACCT GAGGAAGAAG CACAAGCGCC TGGAAGCAGA ACTGGCTGCG 
CATGAGCCGG CTATTCAGGG TGTCCTGGAC ACTGGCAAGA AGCTGTCCGA TGATAACACC 
ATCGGGAAAG AGGAGATCCA GCAGCGGCTG GCGCAGTTTG TGGAGCACTG GAAAGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGTCAGCGG CTGGAAGAGT CCTTGGAATA TCAGCAGTTT 
GTAGCCAATG TGGAAGAGGA GGAAGCCTGG ATCAATGAGA AAATGACCCT GGTGGCCAGC 
GAAGATTATG GCGACACTCT TGCTGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACCGTCCA CAAGGATCGC GTGAATGACG TCTGCACCAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGCCTGAAC 
GGGAAAGTGT CAGACCTGGA GAAAGCTGCA GCCCAGAGAA AGGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA CTGGAAGGCG GACGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGC CGAGACCTGT CTTCTGTGCA GACGCTCCTC 
ACCAAACAGG AAACTTTTGA CGCTGGGCTG CAGGCCTTCC AGCAGGAAGG CATTGCCAAC 
ATCACTGCCC TCAAAGATCA GCTTCTCGCC GCCAAACACG TTCAGTCCAA GGCCATCGAG 
GCCCGGCACG CCTCCCTCAT GAAGAGGTGG AGCCAGCTTC TGGCCAACTC AGCCGCCCGC 
AAGAAGAAGC TTCTGGAGGC GCAGAGTCAC TTCCGCAAGG TGGAGGACCT CTTCCTGACC 
TTTGCCAAAA AGGCTTCTGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCCGTGC GCTGCAACTC CTTGGAAGAA ATCAAAGCTT TGCGCGAGGC CCACGACGCC 
TTCCGCTCCT CCCTCAGCTC TGCCCAGGCT GACTTCAACC AGCTGGCCGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT AGCCTCCAAC CCCTACACCT GGTTTACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT ACAGAAAATC ATCAAGGAGA GGGAGCTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTTGCCCA GCACGCCAAC 
GCCTTCCACC AGTGGATCCA AGAGACCAGG ACATACCTCC TCGATGGGTC CTGTATGGTG 
GAAGAGTCGG GGACCCTCGA ATCCCAGCTT GAAGCTACCA AACGCAAGCA CCAGGAAATC 
CGAGCCATGA GAAGTCAGCT CAAAAAGATC GAGGACCTGG GGGCCGCCAT GGAGGAGGCC 
CTCATCCTGG ACAACAAGTA CACGGAGCAC AGCACCGTGG GCCTCGCCCA GCAGTGGGAC 
CAGCTGGACC AGCTGGGCAT GCGCATGCAG CACAACCTGG AGCAGCAGAT CCAGGCCAGG 
AACACAACAG GTGTGACTGA GGAGGCCCTC AAAGAATTCA GCATGATGTT TAAACACTTT 
GACAAGGACA AGTCTGGCAG GCTGAACCAT CAGGAGTTCA AATCTTGCCT GCGCTCCCTG 
GGCTATGACC TGCCCATGGT GGAGGAAGGG GAACCTGACC CTGAGTTCGA GGCAATCCTG 
GACACGGTGG ATCCGAACAG AGATGGCCAT GTCTCCCTGC AAGAATACAT GGCTTTCATG 
ATCAGCCGCG AAACTGAGAA CGTCAAGTCC AGCGAGGAGA TTGAGAGCGC CTTCCGGGCC 
CTCAGCTCAG AGGGAAAGCC TTACGTGACC AAGGAGGAGC TCTACCAGAA CCTGACCCGG 
GAACAAGCCG ACTACTGCGT CTCCCACATG AAGCCCTACG TGGACGGCAA GGGCCGCGAG 
CTCCCCACCG CGTTCGACTA CGTGGAGTTC ACCCGCTCGC TTTTCGTGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009455700.3
CDS	137..7570
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDIEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRITKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_009457425.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009457425.3

ORF Insert Sequence:

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAA TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AATGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAACACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCCCTGCTAG AGGCAGATGT GGCTGCTCAT CAGGACCGAA TTGATGGCAT TACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATATTG AGGATGAGGA GACGTGGATT 
CGAGAGAAGG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC AGCTGCTTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAGACAC GCATAACTAA GGAGGCCGGC 
AGTGTATCTC TGCGTATGAA GCAGGTGGAA GAACTATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AGGCTCTAAA CACAGACAAT 
TATGGACATG ATCTTGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAATCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCGTT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAAATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACGG CAAGTGATGA GTCATACAAG 
GATCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAGGCTTTT 
GAAGCAGAGC TGCATGCCAA CGCTGACCGG ATCCGTGGGG TTATCGACAT GGGCAACTCC 
CTCATTGAAC GTGGAGCCTG TGCCGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
TTAGCTGACC AGTGGCAATT CTTGGTGCAA AAGTCAGCGG AAAAGAGCCA GAAACTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACA GGGATCAAGG ACTTTGACTT CTGGCTGTCT 
GAGGTGGAGG CCCTGCTGGC ATCCGAAGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC 
CTGCTGAAAA AGCATCAACT GCTGGAAGCA GATATATCTG CCCATGAGGA TCGCCTGAAG 
GACCTGAACA GCCAGGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTA 
AAGGACAAGA GGGACACCAT CAACGGGCGC TTCCAGAAGA TCAAGAGCAT GGCGGCCTCC 
CGGCGAGCCA AGCTGAATGA ATCCCATCGC CTGCACCAGT TCTTCCGGGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGGGCT CGGAGGACTA CGGCCGGGAC 
CTAACCGGCG TGCAGAACCT GAGGAAGAAG CACAAGCGCC TGGAAGCAGA ACTGGCTGCG 
CATGAGCCGG CTATTCAGGG TGTCCTGGAC ACTGGCAAGA AGCTGTCCGA TGATAACACC 
ATCGGGAAAG AGGAGATCCA GCAGCGGCTG GCGCAGTTTG TGGAGCACTG GAAAGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGTCAGCGG CTGGAAGAGT CCTTGGAATA TCAGCAGTTT 
GTAGCCAATG TGGAAGAGGA GGAAGCCTGG ATCAATGAGA AAATGACCCT GGTGGCCAGC 
GAAGATTATG GCGACACTCT TGCTGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACCGTCCA CAAGGATCGC GTGAATGACG TCTGCACCAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGCCTGAAC 
GGGAAAGTGT CAGACCTGGA GAAAGCTGCA GCCCAGAGAA AGGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA CTGGAAGGCG GACGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGC CGAGACCTGT CTTCTGTGCA GACGCTCCTC 
ACCAAACAGG AAACTTTTGA CGCTGGGCTG CAGGCCTTCC AGCAGGAAGG CATTGCCAAC 
ATCACTGCCC TCAAAGATCA GCTTCTCGCC GCCAAACACG TTCAGTCCAA GGCCATCGAG 
GCCCGGCACG CCTCCCTCAT GAAGAGGTGG AGCCAGCTTC TGGCCAACTC AGCCGCCCGC 
AAGAAGAAGC TTCTGGAGGC GCAGAGTCAC TTCCGCAAGG TGGAGGACCT CTTCCTGACC 
TTTGCCAAAA AGGCTTCTGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCCGTGC GCTGCAACTC CTTGGAAGAA ATCAAAGCTT TGCGCGAGGC CCACGACGCC 
TTCCGCTCCT CCCTCAGCTC TGCCCAGGCT GACTTCAACC AGCTGGCCGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT AGCCTCCAAC CCCTACACCT GGTTTACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT ACAGAAAATC ATCAAGGAGA GGGAGCTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTTGCCCA GCACGCCAAC 
GCCTTCCACC AGTGGATCCA AGAGACCAGG ACATACCTCC TCGATGGGTC CTGTATGGTG 
GAAGAGTCGG GGACCCTCGA ATCCCAGCTT GAAGCTACCA AACGCAAGCA CCAGGAAATC 
CGAGCCATGA GAAGTCAGCT CAAAAAGATC GAGGACCTGG GGGCCGCCAT GGAGGAGGCC 
CTCATCCTGG ACAACAAGTA CACGGAGCAC AGCACCGTGG GCCTCGCCCA GCAGTGGGAC 
CAGCTGGACC AGCTGGGCAT GCGCATGCAG CACAACCTGG AGCAGCAGAT CCAGGCCAGG 
AACACAACAG GTGTGACTGA GGAGGCCCTC AAAGAATTCA GCATGATGTT TAAACACTTT 
GACAAGGACA AGTCTGGCAG GCTGAACCAT CAGGAGTTCA AATCTTGCCT GCGCTCCCTG 
GGCTATGACC TGCCCATGGT GGAGGAAGGG GAACCTGACC CTGAGTTCGA GGCAATCCTG 
GACACGGTGG ATCCGAACAG AGATGGCCAT GTCTCCCTGC AAGAATACAT GGCTTTCATG 
ATCAGCCGCG AAACTGAGAA CGTCAAGTCC AGCGAGGAGA TTGAGAGCGC CTTCCGGGCC 
CTCAGCTCAG AGGGAAAGCC TTACGTGACC AAGGAGGAGC TCTACCAGAA CCTGACCCGG 
GAACAAGCCG ACTACTGCGT CTCCCACATG AAGCCCTACG TGGACGGCAA GGGCCGCGAG 
CTCCCCACCG CGTTCGACTA CGTGGAGTTC ACCCGCTCGC TTTTCGTGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_009457427.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009455702.3 Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X10, mRNA.

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CloneID	OPc109765
Clone ID Related Accession (Same CDS sequence)	XM_009457427.3
Accession Version	XM_009457427.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7410bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036888.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009457427.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAATTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAATG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA ACACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCCC TGCTAGAGGC AGATGTGGCT 
GCTCATCAGG ACCGAATTGA TGGCATTACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATATTGAGGA TGAGGAGACG TGGATTCGAG AGAAGGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCAGCT GCTTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAGGC TCTAAACACA GACAATTATG GACATGATCT TGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAAT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCGTTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAAATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACGGCAAG TGATGAGTCA TACAAGGATC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCACCA GAAGCACCAG GCTTTTGAAG CAGAGCTGCA TGCCAACGCT 
GACCGGATCC GTGGGGTTAT CGACATGGGC AACTCCCTCA TTGAACGTGG AGCCTGTGCC 
GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCTTAG CTGACCAGTG GCAATTCTTG 
GTGCAAAAGT CAGCGGAAAA GAGCCAGAAA CTGAAAGAAG CCAACAAGCA GCAGAACTTC 
AACACAGGGA TCAAGGACTT TGACTTCTGG CTGTCTGAGG TGGAGGCCCT GCTGGCATCC 
GAAGATTATG GCAAAGACCT GGCTTCTGTG AACAACCTGC TGAAAAAGCA TCAACTGCTG 
GAAGCAGATA TATCTGCCCA TGAGGATCGC CTGAAGGACC TGAACAGCCA GGCAGACAGC 
CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTAAAGG ACAAGAGGGA CACCATCAAC 
GGGCGCTTCC AGAAGATCAA GAGCATGGCG GCCTCCCGGC GAGCCAAGCT GAATGAATCC 
CATCGCCTGC ACCAGTTCTT CCGGGACATG GATGACGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGGGCTCGGA GGACTACGGC CGGGACCTAA CCGGCGTGCA GAACCTGAGG 
AAGAAGCACA AGCGCCTGGA AGCAGAACTG GCTGCGCATG AGCCGGCTAT TCAGGGTGTC 
CTGGACACTG GCAAGAAGCT GTCCGATGAT AACACCATCG GGAAAGAGGA GATCCAGCAG 
CGGCTGGCGC AGTTTGTGGA GCACTGGAAA GAGCTGAAGC AGCTGGCAGC TGCCCGGGGT 
CAGCGGCTGG AAGAGTCCTT GGAATATCAG CAGTTTGTAG CCAATGTGGA AGAGGAGGAA 
GCCTGGATCA ATGAGAAAAT GACCCTGGTG GCCAGCGAAG ATTATGGCGA CACTCTTGCT 
GCCATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC CGTCCACAAG 
GATCGCGTGA ATGACGTCTG CACCAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCAAA GATGAAGGGC CTGAACGGGA AAGTGTCAGA CCTGGAGAAA 
GCTGCAGCCC AGAGAAAGGC GAAGCTGGAT GAGAACTCGG CCTTCCTTCA GTTCAACTGG 
AAGGCGGACG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGCCGAG ACCTGTCTTC TGTGCAGACG CTCCTCACCA AACAGGAAAC TTTTGACGCT 
GGGCTGCAGG CCTTCCAGCA GGAAGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTT 
CTCGCCGCCA AACACGTTCA GTCCAAGGCC ATCGAGGCCC GGCACGCCTC CCTCATGAAG 
AGGTGGAGCC AGCTTCTGGC CAACTCAGCC GCCCGCAAGA AGAAGCTTCT GGAGGCGCAG 
AGTCACTTCC GCAAGGTGGA GGACCTCTTC CTGACCTTTG CCAAAAAGGC TTCTGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CCGTGCGCTG CAACTCCTTG 
GAAGAAATCA AAGCTTTGCG CGAGGCCCAC GACGCCTTCC GCTCCTCCCT CAGCTCTGCC 
CAGGCTGACT TCAACCAGCT GGCCGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTAGCC 
TCCAACCCCT ACACCTGGTT TACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTACAG 
AAAATCATCA AGGAGAGGGA GCTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT TGCCCAGCAC GCCAACGCCT TCCACCAGTG GATCCAAGAG 
ACCAGGACAT ACCTCCTCGA TGGGTCCTGT ATGGTGGAAG AGTCGGGGAC CCTCGAATCC 
CAGCTTGAAG CTACCAAACG CAAGCACCAG GAAATCCGAG CCATGAGAAG TCAGCTCAAA 
AAGATCGAGG ACCTGGGGGC CGCCATGGAG GAGGCCCTCA TCCTGGACAA CAAGTACACG 
GAGCACAGCA CCGTGGGCCT CGCCCAGCAG TGGGACCAGC TGGACCAGCT GGGCATGCGC 
ATGCAGCACA ACCTGGAGCA GCAGATCCAG GCCAGGAACA CAACAGGTGT GACTGAGGAG 
GCCCTCAAAG AATTCAGCAT GATGTTTAAA CACTTTGACA AGGACAAGTC TGGCAGGCTG 
AACCATCAGG AGTTCAAATC TTGCCTGCGC TCCCTGGGCT ATGACCTGCC CATGGTGGAG 
GAAGGGGAAC CTGACCCTGA GTTCGAGGCA ATCCTGGACA CGGTGGATCC GAACAGAGAT 
GGCCATGTCT CCCTGCAAGA ATACATGGCT TTCATGATCA GCCGCGAAAC TGAGAACGTC 
AAGTCCAGCG AGGAGATTGA GAGCGCCTTC CGGGCCCTCA GCTCAGAGGG AAAGCCTTAC 
GTGACCAAGG AGGAGCTCTA CCAGAACCTG ACCCGGGAAC AAGCCGACTA CTGCGTCTCC 
CACATGAAGC CCTACGTGGA CGGCAAGGGC CGCGAGCTCC CCACCGCGTT CGACTACGTG 
GAGTTCACCC GCTCGCTTTT CGTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009455702.3
CDS	121..7530
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDIEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_009457427.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009457427.3

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAATTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAATG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA ACACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCCC TGCTAGAGGC AGATGTGGCT 
GCTCATCAGG ACCGAATTGA TGGCATTACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATATTGAGGA TGAGGAGACG TGGATTCGAG AGAAGGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCAGCT GCTTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAGGC TCTAAACACA GACAATTATG GACATGATCT TGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAAT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCGTTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAAATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACGGCAAG TGATGAGTCA TACAAGGATC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCACCA GAAGCACCAG GCTTTTGAAG CAGAGCTGCA TGCCAACGCT 
GACCGGATCC GTGGGGTTAT CGACATGGGC AACTCCCTCA TTGAACGTGG AGCCTGTGCC 
GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCTTAG CTGACCAGTG GCAATTCTTG 
GTGCAAAAGT CAGCGGAAAA GAGCCAGAAA CTGAAAGAAG CCAACAAGCA GCAGAACTTC 
AACACAGGGA TCAAGGACTT TGACTTCTGG CTGTCTGAGG TGGAGGCCCT GCTGGCATCC 
GAAGATTATG GCAAAGACCT GGCTTCTGTG AACAACCTGC TGAAAAAGCA TCAACTGCTG 
GAAGCAGATA TATCTGCCCA TGAGGATCGC CTGAAGGACC TGAACAGCCA GGCAGACAGC 
CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTAAAGG ACAAGAGGGA CACCATCAAC 
GGGCGCTTCC AGAAGATCAA GAGCATGGCG GCCTCCCGGC GAGCCAAGCT GAATGAATCC 
CATCGCCTGC ACCAGTTCTT CCGGGACATG GATGACGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGGGCTCGGA GGACTACGGC CGGGACCTAA CCGGCGTGCA GAACCTGAGG 
AAGAAGCACA AGCGCCTGGA AGCAGAACTG GCTGCGCATG AGCCGGCTAT TCAGGGTGTC 
CTGGACACTG GCAAGAAGCT GTCCGATGAT AACACCATCG GGAAAGAGGA GATCCAGCAG 
CGGCTGGCGC AGTTTGTGGA GCACTGGAAA GAGCTGAAGC AGCTGGCAGC TGCCCGGGGT 
CAGCGGCTGG AAGAGTCCTT GGAATATCAG CAGTTTGTAG CCAATGTGGA AGAGGAGGAA 
GCCTGGATCA ATGAGAAAAT GACCCTGGTG GCCAGCGAAG ATTATGGCGA CACTCTTGCT 
GCCATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC CGTCCACAAG 
GATCGCGTGA ATGACGTCTG CACCAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCAAA GATGAAGGGC CTGAACGGGA AAGTGTCAGA CCTGGAGAAA 
GCTGCAGCCC AGAGAAAGGC GAAGCTGGAT GAGAACTCGG CCTTCCTTCA GTTCAACTGG 
AAGGCGGACG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGCCGAG ACCTGTCTTC TGTGCAGACG CTCCTCACCA AACAGGAAAC TTTTGACGCT 
GGGCTGCAGG CCTTCCAGCA GGAAGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTT 
CTCGCCGCCA AACACGTTCA GTCCAAGGCC ATCGAGGCCC GGCACGCCTC CCTCATGAAG 
AGGTGGAGCC AGCTTCTGGC CAACTCAGCC GCCCGCAAGA AGAAGCTTCT GGAGGCGCAG 
AGTCACTTCC GCAAGGTGGA GGACCTCTTC CTGACCTTTG CCAAAAAGGC TTCTGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CCGTGCGCTG CAACTCCTTG 
GAAGAAATCA AAGCTTTGCG CGAGGCCCAC GACGCCTTCC GCTCCTCCCT CAGCTCTGCC 
CAGGCTGACT TCAACCAGCT GGCCGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTAGCC 
TCCAACCCCT ACACCTGGTT TACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTACAG 
AAAATCATCA AGGAGAGGGA GCTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT TGCCCAGCAC GCCAACGCCT TCCACCAGTG GATCCAAGAG 
ACCAGGACAT ACCTCCTCGA TGGGTCCTGT ATGGTGGAAG AGTCGGGGAC CCTCGAATCC 
CAGCTTGAAG CTACCAAACG CAAGCACCAG GAAATCCGAG CCATGAGAAG TCAGCTCAAA 
AAGATCGAGG ACCTGGGGGC CGCCATGGAG GAGGCCCTCA TCCTGGACAA CAAGTACACG 
GAGCACAGCA CCGTGGGCCT CGCCCAGCAG TGGGACCAGC TGGACCAGCT GGGCATGCGC 
ATGCAGCACA ACCTGGAGCA GCAGATCCAG GCCAGGAACA CAACAGGTGT GACTGAGGAG 
GCCCTCAAAG AATTCAGCAT GATGTTTAAA CACTTTGACA AGGACAAGTC TGGCAGGCTG 
AACCATCAGG AGTTCAAATC TTGCCTGCGC TCCCTGGGCT ATGACCTGCC CATGGTGGAG 
GAAGGGGAAC CTGACCCTGA GTTCGAGGCA ATCCTGGACA CGGTGGATCC GAACAGAGAT 
GGCCATGTCT CCCTGCAAGA ATACATGGCT TTCATGATCA GCCGCGAAAC TGAGAACGTC 
AAGTCCAGCG AGGAGATTGA GAGCGCCTTC CGGGCCCTCA GCTCAGAGGG AAAGCCTTAC 
GTGACCAAGG AGGAGCTCTA CCAGAACCTG ACCCGGGAAC AAGCCGACTA CTGCGTCTCC 
CACATGAAGC CCTACGTGGA CGGCAAGGGC CGCGAGCTCC CCACCGCGTT CGACTACGTG 
GAGTTCACCC GCTCGCTTTT CGTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_016961798.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016817287.2 Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X11, mRNA.

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CloneID	OPc109766
Clone ID Related Accession (Same CDS sequence)	XM_016961798.2
Accession Version	XM_016961798.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7395bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036888.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016961798.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAATTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAATG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA ACACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCCC TGCTAGAGGC AGATGTGGCT 
GCTCATCAGG ACCGAATTGA TGGCATTACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATATTGAGGA TGAGGAGACG TGGATTCGAG AGAAGGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCAGCT GCTTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAGGC TCTAAACACA GACAATTATG GACATGATCT TGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAAT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCGTTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAAATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACGGCAAG TGATGAGTCA TACAAGGATC CCACCAACAT CCAGAGCAAG 
CACCAGAAGC ACCAGGCTTT TGAAGCAGAG CTGCATGCCA ACGCTGACCG GATCCGTGGG 
GTTATCGACA TGGGCAACTC CCTCATTGAA CGTGGAGCCT GTGCCGGCAG TGAGGATGCT 
GTCAAGGCCC GCCTGGCTGC CTTAGCTGAC CAGTGGCAAT TCTTGGTGCA AAAGTCAGCG 
GAAAAGAGCC AGAAACTGAA AGAAGCCAAC AAGCAGCAGA ACTTCAACAC AGGGATCAAG 
GACTTTGACT TCTGGCTGTC TGAGGTGGAG GCCCTGCTGG CATCCGAAGA TTATGGCAAA 
GACCTGGCTT CTGTGAACAA CCTGCTGAAA AAGCATCAAC TGCTGGAAGC AGATATATCT 
GCCCATGAGG ATCGCCTGAA GGACCTGAAC AGCCAGGCAG ACAGCCTGAT GACCAGCAGT 
GCCTTCGACA CCTCCCAAGT AAAGGACAAG AGGGACACCA TCAACGGGCG CTTCCAGAAG 
ATCAAGAGCA TGGCGGCCTC CCGGCGAGCC AAGCTGAATG AATCCCATCG CCTGCACCAG 
TTCTTCCGGG ACATGGATGA CGAGGAGTCC TGGATCAAGG AGAAGAAGCT GCTGGTGGGC 
TCGGAGGACT ACGGCCGGGA CCTAACCGGC GTGCAGAACC TGAGGAAGAA GCACAAGCGC 
CTGGAAGCAG AACTGGCTGC GCATGAGCCG GCTATTCAGG GTGTCCTGGA CACTGGCAAG 
AAGCTGTCCG ATGATAACAC CATCGGGAAA GAGGAGATCC AGCAGCGGCT GGCGCAGTTT 
GTGGAGCACT GGAAAGAGCT GAAGCAGCTG GCAGCTGCCC GGGGTCAGCG GCTGGAAGAG 
TCCTTGGAAT ATCAGCAGTT TGTAGCCAAT GTGGAAGAGG AGGAAGCCTG GATCAATGAG 
AAAATGACCC TGGTGGCCAG CGAAGATTAT GGCGACACTC TTGCTGCCAT CCAGGGCTTA 
CTGAAGAAAC ATGAAGCTTT TGAGACAGAC TTCACCGTCC ACAAGGATCG CGTGAATGAC 
GTCTGCACCA ATGGACAAGA CCTCATTAAG AAGAACAATC ACCATGAGGA GAACATCTCT 
TCAAAGATGA AGGGCCTGAA CGGGAAAGTG TCAGACCTGG AGAAAGCTGC AGCCCAGAGA 
AAGGCGAAGC TGGATGAGAA CTCGGCCTTC CTTCAGTTCA ACTGGAAGGC GGACGTGGTG 
GAGTCCTGGA TCGGTGAAAA GGAGAACAGC TTGAAGACAG ATGATTATGG CCGAGACCTG 
TCTTCTGTGC AGACGCTCCT CACCAAACAG GAAACTTTTG ACGCTGGGCT GCAGGCCTTC 
CAGCAGGAAG GCATTGCCAA CATCACTGCC CTCAAAGATC AGCTTCTCGC CGCCAAACAC 
GTTCAGTCCA AGGCCATCGA GGCCCGGCAC GCCTCCCTCA TGAAGAGGTG GAGCCAGCTT 
CTGGCCAACT CAGCCGCCCG CAAGAAGAAG CTTCTGGAGG CGCAGAGTCA CTTCCGCAAG 
GTGGAGGACC TCTTCCTGAC CTTTGCCAAA AAGGCTTCTG CCTTCAACAG CTGGTTTGAA 
AATGCAGAGG AGGACTTAAC AGACCCCGTG CGCTGCAACT CCTTGGAAGA AATCAAAGCT 
TTGCGCGAGG CCCACGACGC CTTCCGCTCC TCCCTCAGCT CTGCCCAGGC TGACTTCAAC 
CAGCTGGCCG AGCTGGACCG CCAGATCAAG AGCTTCCGCG TAGCCTCCAA CCCCTACACC 
TGGTTTACCA TGGAGGCCCT GGAGGAGACC TGGAGGAACC TACAGAAAAT CATCAAGGAG 
AGGGAGCTGG AGCTGCAGAA GGAACAGCGG CGGCAGGAGG AGAACGACAA GCTGCGCCAG 
GAGTTTGCCC AGCACGCCAA CGCCTTCCAC CAGTGGATCC AAGAGACCAG GACATACCTC 
CTCGATGGGT CCTGTATGGT GGAAGAGTCG GGGACCCTCG AATCCCAGCT TGAAGCTACC 
AAACGCAAGC ACCAGGAAAT CCGAGCCATG AGAAGTCAGC TCAAAAAGAT CGAGGACCTG 
GGGGCCGCCA TGGAGGAGGC CCTCATCCTG GACAACAAGT ACACGGAGCA CAGCACCGTG 
GGCCTCGCCC AGCAGTGGGA CCAGCTGGAC CAGCTGGGCA TGCGCATGCA GCACAACCTG 
GAGCAGCAGA TCCAGGCCAG GAACACAACA GGTGTGACTG AGGAGGCCCT CAAAGAATTC 
AGCATGATGT TTAAACACTT TGACAAGGAC AAGTCTGGCA GGCTGAACCA TCAGGAGTTC 
AAATCTTGCC TGCGCTCCCT GGGCTATGAC CTGCCCATGG TGGAGGAAGG GGAACCTGAC 
CCTGAGTTCG AGGCAATCCT GGACACGGTG GATCCGAACA GAGATGGCCA TGTCTCCCTG 
CAAGAATACA TGGCTTTCAT GATCAGCCGC GAAACTGAGA ACGTCAAGTC CAGCGAGGAG 
ATTGAGAGCG CCTTCCGGGC CCTCAGCTCA GAGGGAAAGC CTTACGTGAC CAAGGAGGAG 
CTCTACCAGA ACCTGACCCG GGAACAAGCC GACTACTGCG TCTCCCACAT GAAGCCCTAC 
GTGGACGGCA AGGGCCGCGA GCTCCCCACC GCGTTCGACT ACGTGGAGTT CACCCGCTCG 
CTTTTCGTGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016817287.2
CDS	121..7515
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDIEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_016961798.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016961798.2

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAATTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAATG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA ACACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCCC TGCTAGAGGC AGATGTGGCT 
GCTCATCAGG ACCGAATTGA TGGCATTACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATATTGAGGA TGAGGAGACG TGGATTCGAG AGAAGGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCAGCT GCTTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAGGC TCTAAACACA GACAATTATG GACATGATCT TGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAAT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCGTTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAAATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACGGCAAG TGATGAGTCA TACAAGGATC CCACCAACAT CCAGAGCAAG 
CACCAGAAGC ACCAGGCTTT TGAAGCAGAG CTGCATGCCA ACGCTGACCG GATCCGTGGG 
GTTATCGACA TGGGCAACTC CCTCATTGAA CGTGGAGCCT GTGCCGGCAG TGAGGATGCT 
GTCAAGGCCC GCCTGGCTGC CTTAGCTGAC CAGTGGCAAT TCTTGGTGCA AAAGTCAGCG 
GAAAAGAGCC AGAAACTGAA AGAAGCCAAC AAGCAGCAGA ACTTCAACAC AGGGATCAAG 
GACTTTGACT TCTGGCTGTC TGAGGTGGAG GCCCTGCTGG CATCCGAAGA TTATGGCAAA 
GACCTGGCTT CTGTGAACAA CCTGCTGAAA AAGCATCAAC TGCTGGAAGC AGATATATCT 
GCCCATGAGG ATCGCCTGAA GGACCTGAAC AGCCAGGCAG ACAGCCTGAT GACCAGCAGT 
GCCTTCGACA CCTCCCAAGT AAAGGACAAG AGGGACACCA TCAACGGGCG CTTCCAGAAG 
ATCAAGAGCA TGGCGGCCTC CCGGCGAGCC AAGCTGAATG AATCCCATCG CCTGCACCAG 
TTCTTCCGGG ACATGGATGA CGAGGAGTCC TGGATCAAGG AGAAGAAGCT GCTGGTGGGC 
TCGGAGGACT ACGGCCGGGA CCTAACCGGC GTGCAGAACC TGAGGAAGAA GCACAAGCGC 
CTGGAAGCAG AACTGGCTGC GCATGAGCCG GCTATTCAGG GTGTCCTGGA CACTGGCAAG 
AAGCTGTCCG ATGATAACAC CATCGGGAAA GAGGAGATCC AGCAGCGGCT GGCGCAGTTT 
GTGGAGCACT GGAAAGAGCT GAAGCAGCTG GCAGCTGCCC GGGGTCAGCG GCTGGAAGAG 
TCCTTGGAAT ATCAGCAGTT TGTAGCCAAT GTGGAAGAGG AGGAAGCCTG GATCAATGAG 
AAAATGACCC TGGTGGCCAG CGAAGATTAT GGCGACACTC TTGCTGCCAT CCAGGGCTTA 
CTGAAGAAAC ATGAAGCTTT TGAGACAGAC TTCACCGTCC ACAAGGATCG CGTGAATGAC 
GTCTGCACCA ATGGACAAGA CCTCATTAAG AAGAACAATC ACCATGAGGA GAACATCTCT 
TCAAAGATGA AGGGCCTGAA CGGGAAAGTG TCAGACCTGG AGAAAGCTGC AGCCCAGAGA 
AAGGCGAAGC TGGATGAGAA CTCGGCCTTC CTTCAGTTCA ACTGGAAGGC GGACGTGGTG 
GAGTCCTGGA TCGGTGAAAA GGAGAACAGC TTGAAGACAG ATGATTATGG CCGAGACCTG 
TCTTCTGTGC AGACGCTCCT CACCAAACAG GAAACTTTTG ACGCTGGGCT GCAGGCCTTC 
CAGCAGGAAG GCATTGCCAA CATCACTGCC CTCAAAGATC AGCTTCTCGC CGCCAAACAC 
GTTCAGTCCA AGGCCATCGA GGCCCGGCAC GCCTCCCTCA TGAAGAGGTG GAGCCAGCTT 
CTGGCCAACT CAGCCGCCCG CAAGAAGAAG CTTCTGGAGG CGCAGAGTCA CTTCCGCAAG 
GTGGAGGACC TCTTCCTGAC CTTTGCCAAA AAGGCTTCTG CCTTCAACAG CTGGTTTGAA 
AATGCAGAGG AGGACTTAAC AGACCCCGTG CGCTGCAACT CCTTGGAAGA AATCAAAGCT 
TTGCGCGAGG CCCACGACGC CTTCCGCTCC TCCCTCAGCT CTGCCCAGGC TGACTTCAAC 
CAGCTGGCCG AGCTGGACCG CCAGATCAAG AGCTTCCGCG TAGCCTCCAA CCCCTACACC 
TGGTTTACCA TGGAGGCCCT GGAGGAGACC TGGAGGAACC TACAGAAAAT CATCAAGGAG 
AGGGAGCTGG AGCTGCAGAA GGAACAGCGG CGGCAGGAGG AGAACGACAA GCTGCGCCAG 
GAGTTTGCCC AGCACGCCAA CGCCTTCCAC CAGTGGATCC AAGAGACCAG GACATACCTC 
CTCGATGGGT CCTGTATGGT GGAAGAGTCG GGGACCCTCG AATCCCAGCT TGAAGCTACC 
AAACGCAAGC ACCAGGAAAT CCGAGCCATG AGAAGTCAGC TCAAAAAGAT CGAGGACCTG 
GGGGCCGCCA TGGAGGAGGC CCTCATCCTG GACAACAAGT ACACGGAGCA CAGCACCGTG 
GGCCTCGCCC AGCAGTGGGA CCAGCTGGAC CAGCTGGGCA TGCGCATGCA GCACAACCTG 
GAGCAGCAGA TCCAGGCCAG GAACACAACA GGTGTGACTG AGGAGGCCCT CAAAGAATTC 
AGCATGATGT TTAAACACTT TGACAAGGAC AAGTCTGGCA GGCTGAACCA TCAGGAGTTC 
AAATCTTGCC TGCGCTCCCT GGGCTATGAC CTGCCCATGG TGGAGGAAGG GGAACCTGAC 
CCTGAGTTCG AGGCAATCCT GGACACGGTG GATCCGAACA GAGATGGCCA TGTCTCCCTG 
CAAGAATACA TGGCTTTCAT GATCAGCCGC GAAACTGAGA ACGTCAAGTC CAGCGAGGAG 
ATTGAGAGCG CCTTCCGGGC CCTCAGCTCA GAGGGAAAGC CTTACGTGAC CAAGGAGGAG 
CTCTACCAGA ACCTGACCCG GGAACAAGCC GACTACTGCG TCTCCCACAT GAAGCCCTAC 
GTGGACGGCA AGGGCCGCGA GCTCCCCACC GCGTTCGACT ACGTGGAGTT CACCCGCTCG 
CTTTTCGTGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_009457429.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009455704.3 Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X12, mRNA.

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CloneID	OPc109767
Clone ID Related Accession (Same CDS sequence)	XM_009457429.3
Accession Version	XM_009457429.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7359bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036888.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009457429.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAA TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AATGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAACACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCCCTGCTAG AGGCAGATGT GGCTGCTCAT CAGGACCGAA TTGATGGCAT TACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATATTG AGGATGAGGA GACGTGGATT 
CGAGAGAAGG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC AGCTGCTTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AGGCTCTAAA CACAGACAAT 
TATGGACATG ATCTTGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAATCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCGTT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAAATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACGG CAAGTGATGA GTCATACAAG 
GATCCCACCA ACATCCAGAG CAAGCACCAG AAGCACCAGG CTTTTGAAGC AGAGCTGCAT 
GCCAACGCTG ACCGGATCCG TGGGGTTATC GACATGGGCA ACTCCCTCAT TGAACGTGGA 
GCCTGTGCCG GCAGTGAGGA TGCTGTCAAG GCCCGCCTGG CTGCCTTAGC TGACCAGTGG 
CAATTCTTGG TGCAAAAGTC AGCGGAAAAG AGCCAGAAAC TGAAAGAAGC CAACAAGCAG 
CAGAACTTCA ACACAGGGAT CAAGGACTTT GACTTCTGGC TGTCTGAGGT GGAGGCCCTG 
CTGGCATCCG AAGATTATGG CAAAGACCTG GCTTCTGTGA ACAACCTGCT GAAAAAGCAT 
CAACTGCTGG AAGCAGATAT ATCTGCCCAT GAGGATCGCC TGAAGGACCT GAACAGCCAG 
GCAGACAGCC TGATGACCAG CAGTGCCTTC GACACCTCCC AAGTAAAGGA CAAGAGGGAC 
ACCATCAACG GGCGCTTCCA GAAGATCAAG AGCATGGCGG CCTCCCGGCG AGCCAAGCTG 
AATGAATCCC ATCGCCTGCA CCAGTTCTTC CGGGACATGG ATGACGAGGA GTCCTGGATC 
AAGGAGAAGA AGCTGCTGGT GGGCTCGGAG GACTACGGCC GGGACCTAAC CGGCGTGCAG 
AACCTGAGGA AGAAGCACAA GCGCCTGGAA GCAGAACTGG CTGCGCATGA GCCGGCTATT 
CAGGGTGTCC TGGACACTGG CAAGAAGCTG TCCGATGATA ACACCATCGG GAAAGAGGAG 
ATCCAGCAGC GGCTGGCGCA GTTTGTGGAG CACTGGAAAG AGCTGAAGCA GCTGGCAGCT 
GCCCGGGGTC AGCGGCTGGA AGAGTCCTTG GAATATCAGC AGTTTGTAGC CAATGTGGAA 
GAGGAGGAAG CCTGGATCAA TGAGAAAATG ACCCTGGTGG CCAGCGAAGA TTATGGCGAC 
ACTCTTGCTG CCATCCAGGG CTTACTGAAG AAACATGAAG CTTTTGAGAC AGACTTCACC 
GTCCACAAGG ATCGCGTGAA TGACGTCTGC ACCAATGGAC AAGACCTCAT TAAGAAGAAC 
AATCACCATG AGGAGAACAT CTCTTCAAAG ATGAAGGGCC TGAACGGGAA AGTGTCAGAC 
CTGGAGAAAG CTGCAGCCCA GAGAAAGGCG AAGCTGGATG AGAACTCGGC CTTCCTTCAG 
TTCAACTGGA AGGCGGACGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG 
ACAGATGATT ATGGCCGAGA CCTGTCTTCT GTGCAGACGC TCCTCACCAA ACAGGAAACT 
TTTGACGCTG GGCTGCAGGC CTTCCAGCAG GAAGGCATTG CCAACATCAC TGCCCTCAAA 
GATCAGCTTC TCGCCGCCAA ACACGTTCAG TCCAAGGCCA TCGAGGCCCG GCACGCCTCC 
CTCATGAAGA GGTGGAGCCA GCTTCTGGCC AACTCAGCCG CCCGCAAGAA GAAGCTTCTG 
GAGGCGCAGA GTCACTTCCG CAAGGTGGAG GACCTCTTCC TGACCTTTGC CAAAAAGGCT 
TCTGCCTTCA ACAGCTGGTT TGAAAATGCA GAGGAGGACT TAACAGACCC CGTGCGCTGC 
AACTCCTTGG AAGAAATCAA AGCTTTGCGC GAGGCCCACG ACGCCTTCCG CTCCTCCCTC 
AGCTCTGCCC AGGCTGACTT CAACCAGCTG GCCGAGCTGG ACCGCCAGAT CAAGAGCTTC 
CGCGTAGCCT CCAACCCCTA CACCTGGTTT ACCATGGAGG CCCTGGAGGA GACCTGGAGG 
AACCTACAGA AAATCATCAA GGAGAGGGAG CTGGAGCTGC AGAAGGAACA GCGGCGGCAG 
GAGGAGAACG ACAAGCTGCG CCAGGAGTTT GCCCAGCACG CCAACGCCTT CCACCAGTGG 
ATCCAAGAGA CCAGGACATA CCTCCTCGAT GGGTCCTGTA TGGTGGAAGA GTCGGGGACC 
CTCGAATCCC AGCTTGAAGC TACCAAACGC AAGCACCAGG AAATCCGAGC CATGAGAAGT 
CAGCTCAAAA AGATCGAGGA CCTGGGGGCC GCCATGGAGG AGGCCCTCAT CCTGGACAAC 
AAGTACACGG AGCACAGCAC CGTGGGCCTC GCCCAGCAGT GGGACCAGCT GGACCAGCTG 
GGCATGCGCA TGCAGCACAA CCTGGAGCAG CAGATCCAGG CCAGGAACAC AACAGGTGTG 
ACTGAGGAGG CCCTCAAAGA ATTCAGCATG ATGTTTAAAC ACTTTGACAA GGACAAGTCT 
GGCAGGCTGA ACCATCAGGA GTTCAAATCT TGCCTGCGCT CCCTGGGCTA TGACCTGCCC 
ATGGTGGAGG AAGGGGAACC TGACCCTGAG TTCGAGGCAA TCCTGGACAC GGTGGATCCG 
AACAGAGATG GCCATGTCTC CCTGCAAGAA TACATGGCTT TCATGATCAG CCGCGAAACT 
GAGAACGTCA AGTCCAGCGA GGAGATTGAG AGCGCCTTCC GGGCCCTCAG CTCAGAGGGA 
AAGCCTTACG TGACCAAGGA GGAGCTCTAC CAGAACCTGA CCCGGGAACA AGCCGACTAC 
TGCGTCTCCC ACATGAAGCC CTACGTGGAC GGCAAGGGCC GCGAGCTCCC CACCGCGTTC 
GACTACGTGG AGTTCACCCG CTCGCTTTTC GTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009455704.3
CDS	137..7495
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDIEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_009457429.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009457429.3

ORF Insert Sequence:

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAA TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AATGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAACACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCCCTGCTAG AGGCAGATGT GGCTGCTCAT CAGGACCGAA TTGATGGCAT TACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATATTG AGGATGAGGA GACGTGGATT 
CGAGAGAAGG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC AGCTGCTTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AGGCTCTAAA CACAGACAAT 
TATGGACATG ATCTTGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAATCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCGTT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAAATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACGG CAAGTGATGA GTCATACAAG 
GATCCCACCA ACATCCAGAG CAAGCACCAG AAGCACCAGG CTTTTGAAGC AGAGCTGCAT 
GCCAACGCTG ACCGGATCCG TGGGGTTATC GACATGGGCA ACTCCCTCAT TGAACGTGGA 
GCCTGTGCCG GCAGTGAGGA TGCTGTCAAG GCCCGCCTGG CTGCCTTAGC TGACCAGTGG 
CAATTCTTGG TGCAAAAGTC AGCGGAAAAG AGCCAGAAAC TGAAAGAAGC CAACAAGCAG 
CAGAACTTCA ACACAGGGAT CAAGGACTTT GACTTCTGGC TGTCTGAGGT GGAGGCCCTG 
CTGGCATCCG AAGATTATGG CAAAGACCTG GCTTCTGTGA ACAACCTGCT GAAAAAGCAT 
CAACTGCTGG AAGCAGATAT ATCTGCCCAT GAGGATCGCC TGAAGGACCT GAACAGCCAG 
GCAGACAGCC TGATGACCAG CAGTGCCTTC GACACCTCCC AAGTAAAGGA CAAGAGGGAC 
ACCATCAACG GGCGCTTCCA GAAGATCAAG AGCATGGCGG CCTCCCGGCG AGCCAAGCTG 
AATGAATCCC ATCGCCTGCA CCAGTTCTTC CGGGACATGG ATGACGAGGA GTCCTGGATC 
AAGGAGAAGA AGCTGCTGGT GGGCTCGGAG GACTACGGCC GGGACCTAAC CGGCGTGCAG 
AACCTGAGGA AGAAGCACAA GCGCCTGGAA GCAGAACTGG CTGCGCATGA GCCGGCTATT 
CAGGGTGTCC TGGACACTGG CAAGAAGCTG TCCGATGATA ACACCATCGG GAAAGAGGAG 
ATCCAGCAGC GGCTGGCGCA GTTTGTGGAG CACTGGAAAG AGCTGAAGCA GCTGGCAGCT 
GCCCGGGGTC AGCGGCTGGA AGAGTCCTTG GAATATCAGC AGTTTGTAGC CAATGTGGAA 
GAGGAGGAAG CCTGGATCAA TGAGAAAATG ACCCTGGTGG CCAGCGAAGA TTATGGCGAC 
ACTCTTGCTG CCATCCAGGG CTTACTGAAG AAACATGAAG CTTTTGAGAC AGACTTCACC 
GTCCACAAGG ATCGCGTGAA TGACGTCTGC ACCAATGGAC AAGACCTCAT TAAGAAGAAC 
AATCACCATG AGGAGAACAT CTCTTCAAAG ATGAAGGGCC TGAACGGGAA AGTGTCAGAC 
CTGGAGAAAG CTGCAGCCCA GAGAAAGGCG AAGCTGGATG AGAACTCGGC CTTCCTTCAG 
TTCAACTGGA AGGCGGACGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG 
ACAGATGATT ATGGCCGAGA CCTGTCTTCT GTGCAGACGC TCCTCACCAA ACAGGAAACT 
TTTGACGCTG GGCTGCAGGC CTTCCAGCAG GAAGGCATTG CCAACATCAC TGCCCTCAAA 
GATCAGCTTC TCGCCGCCAA ACACGTTCAG TCCAAGGCCA TCGAGGCCCG GCACGCCTCC 
CTCATGAAGA GGTGGAGCCA GCTTCTGGCC AACTCAGCCG CCCGCAAGAA GAAGCTTCTG 
GAGGCGCAGA GTCACTTCCG CAAGGTGGAG GACCTCTTCC TGACCTTTGC CAAAAAGGCT 
TCTGCCTTCA ACAGCTGGTT TGAAAATGCA GAGGAGGACT TAACAGACCC CGTGCGCTGC 
AACTCCTTGG AAGAAATCAA AGCTTTGCGC GAGGCCCACG ACGCCTTCCG CTCCTCCCTC 
AGCTCTGCCC AGGCTGACTT CAACCAGCTG GCCGAGCTGG ACCGCCAGAT CAAGAGCTTC 
CGCGTAGCCT CCAACCCCTA CACCTGGTTT ACCATGGAGG CCCTGGAGGA GACCTGGAGG 
AACCTACAGA AAATCATCAA GGAGAGGGAG CTGGAGCTGC AGAAGGAACA GCGGCGGCAG 
GAGGAGAACG ACAAGCTGCG CCAGGAGTTT GCCCAGCACG CCAACGCCTT CCACCAGTGG 
ATCCAAGAGA CCAGGACATA CCTCCTCGAT GGGTCCTGTA TGGTGGAAGA GTCGGGGACC 
CTCGAATCCC AGCTTGAAGC TACCAAACGC AAGCACCAGG AAATCCGAGC CATGAGAAGT 
CAGCTCAAAA AGATCGAGGA CCTGGGGGCC GCCATGGAGG AGGCCCTCAT CCTGGACAAC 
AAGTACACGG AGCACAGCAC CGTGGGCCTC GCCCAGCAGT GGGACCAGCT GGACCAGCTG 
GGCATGCGCA TGCAGCACAA CCTGGAGCAG CAGATCCAGG CCAGGAACAC AACAGGTGTG 
ACTGAGGAGG CCCTCAAAGA ATTCAGCATG ATGTTTAAAC ACTTTGACAA GGACAAGTCT 
GGCAGGCTGA ACCATCAGGA GTTCAAATCT TGCCTGCGCT CCCTGGGCTA TGACCTGCCC 
ATGGTGGAGG AAGGGGAACC TGACCCTGAG TTCGAGGCAA TCCTGGACAC GGTGGATCCG 
AACAGAGATG GCCATGTCTC CCTGCAAGAA TACATGGCTT TCATGATCAG CCGCGAAACT 
GAGAACGTCA AGTCCAGCGA GGAGATTGAG AGCGCCTTCC GGGCCCTCAG CTCAGAGGGA 
AAGCCTTACG TGACCAAGGA GGAGCTCTAC CAGAACCTGA CCCGGGAACA AGCCGACTAC 
TGCGTCTCCC ACATGAAGCC CTACGTGGAC GGCAAGGGCC GCGAGCTCCC CACCGCGTTC 
GACTACGTGG AGTTCACCCG CTCGCTTTTC GTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_016961799.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016817288.2 Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X13, mRNA.

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CloneID	OPc109768
Clone ID Related Accession (Same CDS sequence)	XM_016961799.2
Accession Version	XM_016961799.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7533bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036888.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016961799.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAATTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAATG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA ACACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCCC TGCTAGAGGC AGATGTGGCT 
GCTCATCAGG ACCGAATTGA TGGCATTACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATATTGAGGA TGAGGAGACG TGGATTCGAG AGAAGGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCAGCT GCTTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AGACACGCAT AACTAAGGAG GCCGGCAGTG TATCTCTGCG TATGAAGCAG 
GTGGAAGAAC TATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAGGC TCTAAACACA GACAATTATG GACATGATCT TGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAAT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCGTTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAAATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACGGCAAG TGATGAGTCA TACAAGGATC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCACCA GAAGCACCAG GCTTTTGAAG CAGAGCTGCA TGCCAACGCT 
GACCGGATCC GTGGGGTTAT CGACATGGGC AACTCCCTCA TTGAACGTGG AGCCTGTGCC 
GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCTTAG CTGACCAGTG GCAATTCTTG 
GTGCAAAAGT CAGCGGAAAA GAGCCAGAAA CTGAAAGAAG CCAACAAGCA GCAGAACTTC 
AACACAGGGA TCAAGGACTT TGACTTCTGG CTGTCTGAGG TGGAGGCCCT GCTGGCATCC 
GAAGATTATG GCAAAGACCT GGCTTCTGTG AACAACCTGC TGAAAAAGCA TCAACTGCTG 
GAAGCAGATA TATCTGCCCA TGAGGATCGC CTGAAGGACC TGAACAGCCA GGCAGACAGC 
CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTAAAGG ACAAGAGGGA CACCATCAAC 
GGGCGCTTCC AGAAGATCAA GAGCATGGCG GCCTCCCGGC GAGCCAAGCT GAATGAATCC 
CATCGCCTGC ACCAGTTCTT CCGGGACATG GATGACGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGGGCTCGGA GGACTACGGC CGGGACCTAA CCGGCGTGCA GAACCTGAGG 
AAGAAGCACA AGCGCCTGGA AGCAGAACTG GCTGCGCATG AGCCGGCTAT TCAGGGTGTC 
CTGGACACTG GCAAGAAGCT GTCCGATGAT AACACCATCG GGAAAGAGGA GATCCAGCAG 
CGGCTGGCGC AGTTTGTGGA GCACTGGAAA GAGCTGAAGC AGCTGGCAGC TGCCCGGGGT 
CAGCGGCTGG AAGAGTCCTT GGAATATCAG CAGTTTGTAG CCAATGTGGA AGAGGAGGAA 
GCCTGGATCA ATGAGAAAAT GACCCTGGTG GCCAGCGAAG ATTATGGCGA CACTCTTGCT 
GCCATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC CGTCCACAAG 
GATCGCGTGA ATGACGTCTG CACCAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCAAA GATGAAGGGC CTGAACGGGA AAGTGTCAGA CCTGGAGAAA 
GCTGCAGCCC AGAGAAAGGC GAAGCTGGAT GAGAACTCGG CCTTCCTTCA GTTCAACTGG 
AAGGCGGACG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGCCGAG ACCTGTCTTC TGTGCAGACG CTCCTCACCA AACAGGAAAC TTTTGACGCT 
GGGCTGCAGG CCTTCCAGCA GGAAGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTT 
CTCGCCGCCA AACACGTTCA GTCCAAGGCC ATCGAGGCCC GGCACGCCTC CCTCATGAAG 
AGGTGGAGCC AGCTTCTGGC CAACTCAGCC GCCCGCAAGA AGAAGCTTCT GGAGGCGCAG 
AGTCACTTCC GCAAGGTGGA GGACCTCTTC CTGACCTTTG CCAAAAAGGC TTCTGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CCGTGCGCTG CAACTCCTTG 
GAAGAAATCA AAGCTTTGCG CGAGGCCCAC GACGCCTTCC GCTCCTCCCT CAGCTCTGCC 
CAGGCTGACT TCAACCAGCT GGCCGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTAGCC 
TCCAACCCCT ACACCTGGTT TACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTACAG 
AAAATCATCA AGGAGAGGGA GCTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT TGCCCAGCAC GCCAACGCCT TCCACCAGTG GATCCAAGAG 
ACCAGGACAT ACCTCCTCGA TGGCATAGCA TATCGTCGGG TCATTCGTGT CTATCAGTAT 
GAAGTTGGGG ATGATCTGTC TGGAAGGTCC TGTATGGTGG AAGAGTCGGG GACCCTCGAA 
TCCCAGCTTG AAGCTACCAA ACGCAAGCAC CAGGAAATCC GAGCCATGAG AAGTCAGCTC 
AAAAAGATCG AGGACCTGGG GGCCGCCATG GAGGAGGCCC TCATCCTGGA CAACAAGTAC 
ACGGAGCACA GCACCGTGGG CCTCGCCCAG CAGTGGGACC AGCTGGACCA GCTGGGCATG 
CGCATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACAACAGG TGTGACTGAG 
GAGGCCCTCA AAGAATTCAG CATGATGTTT AAACACTTTG ACAAGGACAA GTCTGGCAGG 
CTGAACCATC AGGAGTTCAA ATCTTGCCTG CGCTCCCTGG GCTATGACCT GCCCATGGTG 
GAGGAAGGGG AACCTGACCC TGAGTTCGAG GCAATCCTGG ACACGGTGGA TCCGAACAGA 
GATGGCCATG TCTCCCTGCA AGAATACATG GCTTTCATGA TCAGCCGCGA AACTGAGAAC 
GTCAAGTCCA GCGAGGAGAT TGAGAGCGCC TTCCGGGCCC TCAGCTCAGA GGGAAAGCCT 
TACGTGACCA AGGAGGAGCT CTACCAGAAC CTGACCCGGG AACAAGCCGA CTACTGCGTC 
TCCCACATGA AGCCCTACGT GGACGGCAAG GGCCGCGAGC TCCCCACCGC GTTCGACTAC 
GTGGAGTTCA CCCGCTCGCT TTTCGTGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016817288.2
CDS	121..7653
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDIEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRITKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_016961799.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016961799.2

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAATTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAATG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA ACACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCCC TGCTAGAGGC AGATGTGGCT 
GCTCATCAGG ACCGAATTGA TGGCATTACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATATTGAGGA TGAGGAGACG TGGATTCGAG AGAAGGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCAGCT GCTTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AGACACGCAT AACTAAGGAG GCCGGCAGTG TATCTCTGCG TATGAAGCAG 
GTGGAAGAAC TATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAGGC TCTAAACACA GACAATTATG GACATGATCT TGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAAT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCGTTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAAATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACGGCAAG TGATGAGTCA TACAAGGATC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCACCA GAAGCACCAG GCTTTTGAAG CAGAGCTGCA TGCCAACGCT 
GACCGGATCC GTGGGGTTAT CGACATGGGC AACTCCCTCA TTGAACGTGG AGCCTGTGCC 
GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCTTAG CTGACCAGTG GCAATTCTTG 
GTGCAAAAGT CAGCGGAAAA GAGCCAGAAA CTGAAAGAAG CCAACAAGCA GCAGAACTTC 
AACACAGGGA TCAAGGACTT TGACTTCTGG CTGTCTGAGG TGGAGGCCCT GCTGGCATCC 
GAAGATTATG GCAAAGACCT GGCTTCTGTG AACAACCTGC TGAAAAAGCA TCAACTGCTG 
GAAGCAGATA TATCTGCCCA TGAGGATCGC CTGAAGGACC TGAACAGCCA GGCAGACAGC 
CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTAAAGG ACAAGAGGGA CACCATCAAC 
GGGCGCTTCC AGAAGATCAA GAGCATGGCG GCCTCCCGGC GAGCCAAGCT GAATGAATCC 
CATCGCCTGC ACCAGTTCTT CCGGGACATG GATGACGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGGGCTCGGA GGACTACGGC CGGGACCTAA CCGGCGTGCA GAACCTGAGG 
AAGAAGCACA AGCGCCTGGA AGCAGAACTG GCTGCGCATG AGCCGGCTAT TCAGGGTGTC 
CTGGACACTG GCAAGAAGCT GTCCGATGAT AACACCATCG GGAAAGAGGA GATCCAGCAG 
CGGCTGGCGC AGTTTGTGGA GCACTGGAAA GAGCTGAAGC AGCTGGCAGC TGCCCGGGGT 
CAGCGGCTGG AAGAGTCCTT GGAATATCAG CAGTTTGTAG CCAATGTGGA AGAGGAGGAA 
GCCTGGATCA ATGAGAAAAT GACCCTGGTG GCCAGCGAAG ATTATGGCGA CACTCTTGCT 
GCCATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC CGTCCACAAG 
GATCGCGTGA ATGACGTCTG CACCAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCAAA GATGAAGGGC CTGAACGGGA AAGTGTCAGA CCTGGAGAAA 
GCTGCAGCCC AGAGAAAGGC GAAGCTGGAT GAGAACTCGG CCTTCCTTCA GTTCAACTGG 
AAGGCGGACG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGCCGAG ACCTGTCTTC TGTGCAGACG CTCCTCACCA AACAGGAAAC TTTTGACGCT 
GGGCTGCAGG CCTTCCAGCA GGAAGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTT 
CTCGCCGCCA AACACGTTCA GTCCAAGGCC ATCGAGGCCC GGCACGCCTC CCTCATGAAG 
AGGTGGAGCC AGCTTCTGGC CAACTCAGCC GCCCGCAAGA AGAAGCTTCT GGAGGCGCAG 
AGTCACTTCC GCAAGGTGGA GGACCTCTTC CTGACCTTTG CCAAAAAGGC TTCTGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CCGTGCGCTG CAACTCCTTG 
GAAGAAATCA AAGCTTTGCG CGAGGCCCAC GACGCCTTCC GCTCCTCCCT CAGCTCTGCC 
CAGGCTGACT TCAACCAGCT GGCCGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTAGCC 
TCCAACCCCT ACACCTGGTT TACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTACAG 
AAAATCATCA AGGAGAGGGA GCTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT TGCCCAGCAC GCCAACGCCT TCCACCAGTG GATCCAAGAG 
ACCAGGACAT ACCTCCTCGA TGGCATAGCA TATCGTCGGG TCATTCGTGT CTATCAGTAT 
GAAGTTGGGG ATGATCTGTC TGGAAGGTCC TGTATGGTGG AAGAGTCGGG GACCCTCGAA 
TCCCAGCTTG AAGCTACCAA ACGCAAGCAC CAGGAAATCC GAGCCATGAG AAGTCAGCTC 
AAAAAGATCG AGGACCTGGG GGCCGCCATG GAGGAGGCCC TCATCCTGGA CAACAAGTAC 
ACGGAGCACA GCACCGTGGG CCTCGCCCAG CAGTGGGACC AGCTGGACCA GCTGGGCATG 
CGCATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACAACAGG TGTGACTGAG 
GAGGCCCTCA AAGAATTCAG CATGATGTTT AAACACTTTG ACAAGGACAA GTCTGGCAGG 
CTGAACCATC AGGAGTTCAA ATCTTGCCTG CGCTCCCTGG GCTATGACCT GCCCATGGTG 
GAGGAAGGGG AACCTGACCC TGAGTTCGAG GCAATCCTGG ACACGGTGGA TCCGAACAGA 
GATGGCCATG TCTCCCTGCA AGAATACATG GCTTTCATGA TCAGCCGCGA AACTGAGAAC 
GTCAAGTCCA GCGAGGAGAT TGAGAGCGCC TTCCGGGCCC TCAGCTCAGA GGGAAAGCCT 
TACGTGACCA AGGAGGAGCT CTACCAGAAC CTGACCCGGG AACAAGCCGA CTACTGCGTC 
TCCCACATGA AGCCCTACGT GGACGGCAAG GGCCGCGAGC TCCCCACCGC GTTCGACTAC 
GTGGAGTTCA CCCGCTCGCT TTTCGTGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_009457426.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009455701.3 Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X14, mRNA.

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CloneID	OPc109769
Clone ID Related Accession (Same CDS sequence)	XM_009457426.3
Accession Version	XM_009457426.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7419bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036888.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009457426.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAA TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AATGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAACACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCCCTGCTAG AGGCAGATGT GGCTGCTCAT CAGGACCGAA TTGATGGCAT TACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATATTG AGGATGAGGA GACGTGGATT 
CGAGAGAAGG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC AGCTGCTTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAGACAC GCATAACTAA GGAGGCCGGC 
AGTGTATCTC TGCGTATGAA GCAGGTGGAA GAACTATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AGGCTCTAAA CACAGACAAT 
TATGGACATG ATCTTGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAATCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCGTT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAAATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACGG CAAGTGATGA GTCATACAAG 
GATCCCACCA ACATCCAGAG CAAGCACCAG AAGCACCAGG CTTTTGAAGC AGAGCTGCAT 
GCCAACGCTG ACCGGATCCG TGGGGTTATC GACATGGGCA ACTCCCTCAT TGAACGTGGA 
GCCTGTGCCG GCAGTGAGGA TGCTGTCAAG GCCCGCCTGG CTGCCTTAGC TGACCAGTGG 
CAATTCTTGG TGCAAAAGTC AGCGGAAAAG AGCCAGAAAC TGAAAGAAGC CAACAAGCAG 
CAGAACTTCA ACACAGGGAT CAAGGACTTT GACTTCTGGC TGTCTGAGGT GGAGGCCCTG 
CTGGCATCCG AAGATTATGG CAAAGACCTG GCTTCTGTGA ACAACCTGCT GAAAAAGCAT 
CAACTGCTGG AAGCAGATAT ATCTGCCCAT GAGGATCGCC TGAAGGACCT GAACAGCCAG 
GCAGACAGCC TGATGACCAG CAGTGCCTTC GACACCTCCC AAGTAAAGGA CAAGAGGGAC 
ACCATCAACG GGCGCTTCCA GAAGATCAAG AGCATGGCGG CCTCCCGGCG AGCCAAGCTG 
AATGAATCCC ATCGCCTGCA CCAGTTCTTC CGGGACATGG ATGACGAGGA GTCCTGGATC 
AAGGAGAAGA AGCTGCTGGT GGGCTCGGAG GACTACGGCC GGGACCTAAC CGGCGTGCAG 
AACCTGAGGA AGAAGCACAA GCGCCTGGAA GCAGAACTGG CTGCGCATGA GCCGGCTATT 
CAGGGTGTCC TGGACACTGG CAAGAAGCTG TCCGATGATA ACACCATCGG GAAAGAGGAG 
ATCCAGCAGC GGCTGGCGCA GTTTGTGGAG CACTGGAAAG AGCTGAAGCA GCTGGCAGCT 
GCCCGGGGTC AGCGGCTGGA AGAGTCCTTG GAATATCAGC AGTTTGTAGC CAATGTGGAA 
GAGGAGGAAG CCTGGATCAA TGAGAAAATG ACCCTGGTGG CCAGCGAAGA TTATGGCGAC 
ACTCTTGCTG CCATCCAGGG CTTACTGAAG AAACATGAAG CTTTTGAGAC AGACTTCACC 
GTCCACAAGG ATCGCGTGAA TGACGTCTGC ACCAATGGAC AAGACCTCAT TAAGAAGAAC 
AATCACCATG AGGAGAACAT CTCTTCAAAG ATGAAGGGCC TGAACGGGAA AGTGTCAGAC 
CTGGAGAAAG CTGCAGCCCA GAGAAAGGCG AAGCTGGATG AGAACTCGGC CTTCCTTCAG 
TTCAACTGGA AGGCGGACGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG 
ACAGATGATT ATGGCCGAGA CCTGTCTTCT GTGCAGACGC TCCTCACCAA ACAGGAAACT 
TTTGACGCTG GGCTGCAGGC CTTCCAGCAG GAAGGCATTG CCAACATCAC TGCCCTCAAA 
GATCAGCTTC TCGCCGCCAA ACACGTTCAG TCCAAGGCCA TCGAGGCCCG GCACGCCTCC 
CTCATGAAGA GGTGGAGCCA GCTTCTGGCC AACTCAGCCG CCCGCAAGAA GAAGCTTCTG 
GAGGCGCAGA GTCACTTCCG CAAGGTGGAG GACCTCTTCC TGACCTTTGC CAAAAAGGCT 
TCTGCCTTCA ACAGCTGGTT TGAAAATGCA GAGGAGGACT TAACAGACCC CGTGCGCTGC 
AACTCCTTGG AAGAAATCAA AGCTTTGCGC GAGGCCCACG ACGCCTTCCG CTCCTCCCTC 
AGCTCTGCCC AGGCTGACTT CAACCAGCTG GCCGAGCTGG ACCGCCAGAT CAAGAGCTTC 
CGCGTAGCCT CCAACCCCTA CACCTGGTTT ACCATGGAGG CCCTGGAGGA GACCTGGAGG 
AACCTACAGA AAATCATCAA GGAGAGGGAG CTGGAGCTGC AGAAGGAACA GCGGCGGCAG 
GAGGAGAACG ACAAGCTGCG CCAGGAGTTT GCCCAGCACG CCAACGCCTT CCACCAGTGG 
ATCCAAGAGA CCAGGACATA CCTCCTCGAT GGGTCCTGTA TGGTGGAAGA GTCGGGGACC 
CTCGAATCCC AGCTTGAAGC TACCAAACGC AAGCACCAGG AAATCCGAGC CATGAGAAGT 
CAGCTCAAAA AGATCGAGGA CCTGGGGGCC GCCATGGAGG AGGCCCTCAT CCTGGACAAC 
AAGTACACGG AGCACAGCAC CGTGGGCCTC GCCCAGCAGT GGGACCAGCT GGACCAGCTG 
GGCATGCGCA TGCAGCACAA CCTGGAGCAG CAGATCCAGG CCAGGAACAC AACAGGTGTG 
ACTGAGGAGG CCCTCAAAGA ATTCAGCATG ATGTTTAAAC ACTTTGACAA GGACAAGTCT 
GGCAGGCTGA ACCATCAGGA GTTCAAATCT TGCCTGCGCT CCCTGGGCTA TGACCTGCCC 
ATGGTGGAGG AAGGGGAACC TGACCCTGAG TTCGAGGCAA TCCTGGACAC GGTGGATCCG 
AACAGAGATG GCCATGTCTC CCTGCAAGAA TACATGGCTT TCATGATCAG CCGCGAAACT 
GAGAACGTCA AGTCCAGCGA GGAGATTGAG AGCGCCTTCC GGGCCCTCAG CTCAGAGGGA 
AAGCCTTACG TGACCAAGGA GGAGCTCTAC CAGAACCTGA CCCGGGAACA AGCCGACTAC 
TGCGTCTCCC ACATGAAGCC CTACGTGGAC GGCAAGGGCC GCGAGCTCCC CACCGCGTTC 
GACTACGTGG AGTTCACCCG CTCGCTTTTC GTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009455701.3
CDS	137..7555
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDIEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRITKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_009457426.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009457426.3

ORF Insert Sequence:

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAA TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AATGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAACACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCCCTGCTAG AGGCAGATGT GGCTGCTCAT CAGGACCGAA TTGATGGCAT TACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATATTG AGGATGAGGA GACGTGGATT 
CGAGAGAAGG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC AGCTGCTTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAGACAC GCATAACTAA GGAGGCCGGC 
AGTGTATCTC TGCGTATGAA GCAGGTGGAA GAACTATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AGGCTCTAAA CACAGACAAT 
TATGGACATG ATCTTGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAATCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCGTT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAAATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACGG CAAGTGATGA GTCATACAAG 
GATCCCACCA ACATCCAGAG CAAGCACCAG AAGCACCAGG CTTTTGAAGC AGAGCTGCAT 
GCCAACGCTG ACCGGATCCG TGGGGTTATC GACATGGGCA ACTCCCTCAT TGAACGTGGA 
GCCTGTGCCG GCAGTGAGGA TGCTGTCAAG GCCCGCCTGG CTGCCTTAGC TGACCAGTGG 
CAATTCTTGG TGCAAAAGTC AGCGGAAAAG AGCCAGAAAC TGAAAGAAGC CAACAAGCAG 
CAGAACTTCA ACACAGGGAT CAAGGACTTT GACTTCTGGC TGTCTGAGGT GGAGGCCCTG 
CTGGCATCCG AAGATTATGG CAAAGACCTG GCTTCTGTGA ACAACCTGCT GAAAAAGCAT 
CAACTGCTGG AAGCAGATAT ATCTGCCCAT GAGGATCGCC TGAAGGACCT GAACAGCCAG 
GCAGACAGCC TGATGACCAG CAGTGCCTTC GACACCTCCC AAGTAAAGGA CAAGAGGGAC 
ACCATCAACG GGCGCTTCCA GAAGATCAAG AGCATGGCGG CCTCCCGGCG AGCCAAGCTG 
AATGAATCCC ATCGCCTGCA CCAGTTCTTC CGGGACATGG ATGACGAGGA GTCCTGGATC 
AAGGAGAAGA AGCTGCTGGT GGGCTCGGAG GACTACGGCC GGGACCTAAC CGGCGTGCAG 
AACCTGAGGA AGAAGCACAA GCGCCTGGAA GCAGAACTGG CTGCGCATGA GCCGGCTATT 
CAGGGTGTCC TGGACACTGG CAAGAAGCTG TCCGATGATA ACACCATCGG GAAAGAGGAG 
ATCCAGCAGC GGCTGGCGCA GTTTGTGGAG CACTGGAAAG AGCTGAAGCA GCTGGCAGCT 
GCCCGGGGTC AGCGGCTGGA AGAGTCCTTG GAATATCAGC AGTTTGTAGC CAATGTGGAA 
GAGGAGGAAG CCTGGATCAA TGAGAAAATG ACCCTGGTGG CCAGCGAAGA TTATGGCGAC 
ACTCTTGCTG CCATCCAGGG CTTACTGAAG AAACATGAAG CTTTTGAGAC AGACTTCACC 
GTCCACAAGG ATCGCGTGAA TGACGTCTGC ACCAATGGAC AAGACCTCAT TAAGAAGAAC 
AATCACCATG AGGAGAACAT CTCTTCAAAG ATGAAGGGCC TGAACGGGAA AGTGTCAGAC 
CTGGAGAAAG CTGCAGCCCA GAGAAAGGCG AAGCTGGATG AGAACTCGGC CTTCCTTCAG 
TTCAACTGGA AGGCGGACGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG 
ACAGATGATT ATGGCCGAGA CCTGTCTTCT GTGCAGACGC TCCTCACCAA ACAGGAAACT 
TTTGACGCTG GGCTGCAGGC CTTCCAGCAG GAAGGCATTG CCAACATCAC TGCCCTCAAA 
GATCAGCTTC TCGCCGCCAA ACACGTTCAG TCCAAGGCCA TCGAGGCCCG GCACGCCTCC 
CTCATGAAGA GGTGGAGCCA GCTTCTGGCC AACTCAGCCG CCCGCAAGAA GAAGCTTCTG 
GAGGCGCAGA GTCACTTCCG CAAGGTGGAG GACCTCTTCC TGACCTTTGC CAAAAAGGCT 
TCTGCCTTCA ACAGCTGGTT TGAAAATGCA GAGGAGGACT TAACAGACCC CGTGCGCTGC 
AACTCCTTGG AAGAAATCAA AGCTTTGCGC GAGGCCCACG ACGCCTTCCG CTCCTCCCTC 
AGCTCTGCCC AGGCTGACTT CAACCAGCTG GCCGAGCTGG ACCGCCAGAT CAAGAGCTTC 
CGCGTAGCCT CCAACCCCTA CACCTGGTTT ACCATGGAGG CCCTGGAGGA GACCTGGAGG 
AACCTACAGA AAATCATCAA GGAGAGGGAG CTGGAGCTGC AGAAGGAACA GCGGCGGCAG 
GAGGAGAACG ACAAGCTGCG CCAGGAGTTT GCCCAGCACG CCAACGCCTT CCACCAGTGG 
ATCCAAGAGA CCAGGACATA CCTCCTCGAT GGGTCCTGTA TGGTGGAAGA GTCGGGGACC 
CTCGAATCCC AGCTTGAAGC TACCAAACGC AAGCACCAGG AAATCCGAGC CATGAGAAGT 
CAGCTCAAAA AGATCGAGGA CCTGGGGGCC GCCATGGAGG AGGCCCTCAT CCTGGACAAC 
AAGTACACGG AGCACAGCAC CGTGGGCCTC GCCCAGCAGT GGGACCAGCT GGACCAGCTG 
GGCATGCGCA TGCAGCACAA CCTGGAGCAG CAGATCCAGG CCAGGAACAC AACAGGTGTG 
ACTGAGGAGG CCCTCAAAGA ATTCAGCATG ATGTTTAAAC ACTTTGACAA GGACAAGTCT 
GGCAGGCTGA ACCATCAGGA GTTCAAATCT TGCCTGCGCT CCCTGGGCTA TGACCTGCCC 
ATGGTGGAGG AAGGGGAACC TGACCCTGAG TTCGAGGCAA TCCTGGACAC GGTGGATCCG 
AACAGAGATG GCCATGTCTC CCTGCAAGAA TACATGGCTT TCATGATCAG CCGCGAAACT 
GAGAACGTCA AGTCCAGCGA GGAGATTGAG AGCGCCTTCC GGGCCCTCAG CTCAGAGGGA 
AAGCCTTACG TGACCAAGGA GGAGCTCTAC CAGAACCTGA CCCGGGAACA AGCCGACTAC 
TGCGTCTCCC ACATGAAGCC CTACGTGGAC GGCAAGGGCC GCGAGCTCCC CACCGCGTTC 
GACTACGTGG AGTTCACCCG CTCGCTTTTC GTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_009457418.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009455693.2 Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X1, mRNA.

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CloneID	OPc89629
Clone ID Related Accession (Same CDS sequence)	XM_009457418.2
Accession Version	XM_009457418.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7518bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006476.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009457418.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAATTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAATG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA ACACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCCC TGCTAGAGGC AGATGTGGCT 
GCTCATCAGG ACCGAATTGA TGGCATTACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATGTTGAGGA TGAGGAGACG TGGATTCGAG AGAAGGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCAGCT GCTTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AGACACGCAT AACTAAGGAG GCCGGCAGTG TATCTCTGCG TATGAAGCAG 
GTGGAAGAAC TATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAGGC TCTAAACACA GACAATTATG GACATGATCT TGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAAT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCGTTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAAATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACGGCAAG TGATGAGTCA TACAAGGATC CCACCAACAT CCAGAGCAAG 
CACCAGAAGC ACCAGGCTTT TGAAGCAGAG CTGCATGCCA ACGCTGACCG GATCCGTGGG 
GTTATCGACA TGGGCAACTC CCTCATTGAA CGTGGAGCCT GTGCCGGCAG TGAGGATGCT 
GTCAAGGCCC GCCTGGCTGC CTTAGCTGAC CAGTGGCAAT TCTTGGTGCA AAAGTCAGCG 
GAAAAGAGCC AGAAACTGAA AGAAGCCAAC AAGCAGCAGA ACTTCAACAC AGGGATCAAG 
GACTTTGACT TCTGGCTGTC TGAGGTGGAG GCCCTGCTGG CATCCGAAGA TTATGGCAAA 
GACCTGGCTT CTGTGAACAA CCTGCTGAAA AAGCATCAAC TGCTGGAAGC AGATATATCT 
GCCCATGAGG ATCGCCTGAA GGACCTGAAC AGCCAGGCAG ACAGCCTGAT GACCAGCAGT 
GCCTTCGACA CCTCCCAAGT AAAGGACAAG AGGGACACCA TCAACGGGCG CTTCCAGAAG 
ATCAAGAGCA TGGCGGCCTC CCGGCGAGCC AAGCTGAATG AATCCCATCG CCTGCACCAG 
TTCTTCCGGG ACATGGATGA CGAGGAGTCC TGGATCAAGG AGAAGAAGCT GCTGGTGGGC 
TCGGAGGACT ACGGCCGGGA CCTAACCGGC GTGCAGAACC TGAGGAAGAA GCACAAGCGC 
CTGGAAGCAG AACTGGCTGC GCATGAGCCG GCTATTCAGG GTGTCCTGGA CACTGGCAAG 
AAGCTGTCCG ATGATAACAC CATCGGGAAA GAGGAGATCC AGCAGCGGCT GGCGCAGTTT 
GTGGAGCACT GGAAAGAGCT GAAGCAGCTG GCAGCTGCCC GGGGTCAGCG GCTGGAAGAG 
TCCTTGGAAT ATCAGCAGTT TGTAGCCAAT GTGGAAGAGG AGGAAGCCTG GATCAATGAG 
AAAATGACCC TGGTGGCCAG CGAAGATTAT GGCGACACTC TTGCTGCCAT CCAGGGCTTA 
CTGAAGAAAC ATGAAGCTTT TGAGACAGAC TTCACCGTCC ACAAGGATCG CGTGAATGAC 
GTCTGCACCA ATGGACAAGA CCTCATTAAG AAGAACAATC ACCATGAGGA GAACATCTCT 
TCAAAGATGA AGGGCCTGAA CGGGAAAGTG TCAGACCTGG AGAAAGCTGC AGCCCAGAGA 
AAGGCGAAGC TGGATGAGAA CTCGGCCTTC CTTCAGTTCA ACTGGAAGGC GGACGTGGTG 
GAGTCCTGGA TCGGTGAAAA GGAGAACAGC TTGAAGACAG ATGATTATGG CCGAGACCTG 
TCTTCTGTGC AGACGCTCCT CACCAAACAG GAAACTTTTG ACGCTGGGCT GCAGGCCTTC 
CAGCAGGAAG GCATTGCCAA CATCACTGCC CTCAAAGATC AGCTTCTCGC CGCCAAACAC 
GTTCAGTCCA AGGCCATCGA GGCCCGGCAC GCCTCCCTCA TGAAGAGGTG GAGCCAGCTT 
CTGGCCAACT CAGCCGCCCG CAAGAAGAAG CTTCTGGAGG CGCAGAGTCA CTTCCGCAAG 
GTGGAGGACC TCTTCCTGAC CTTTGCCAAA AAGGCTTCTG CCTTCAACAG CTGGTTTGAA 
AATGCAGAGG AGGACTTAAC AGACCCCGTG CGCTGCAACT CCTTGGAAGA AATCAAAGCT 
TTGCGCGAGG CCCACGACGC CTTCCGCTCC TCCCTCAGCT CTGCCCAGGC TGACTTCAAC 
CAGCTGGCCG AGCTGGACCG CCAGATCAAG AGCTTCCGCG TAGCCTCCAA CCCCTACACC 
TGGTTTACCA TGGAGGCCCT GGAGGAGACC TGGAGGAACC TACAGAAAAT CATCAAGGAG 
AGGGAGCTGG AGCTGCAGAA GGAACAGCGG CGGCAGGAGG AGAACGACAA GCTGCGCCAG 
GAGTTTGCCC AGCACGCCAA CGCCTTCCAC CAGTGGATCC AAGAGACCAG GACATACCTC 
CTCGATGGCA TAGCATATCG TCGGGTCATT CGTGTCTATC AGTATGAAGT TGGGGATGAT 
CTGTCTGGAA GGTCCTGTAT GGTGGAAGAG TCGGGGACCC TCGAATCCCA GCTTGAAGCT 
ACCAAACGCA AGCACCAGGA AATCCGAGCC ATGAGAAGTC AGCTCAAAAA GATCGAGGAC 
CTGGGGGCCG CCATGGAGGA GGCCCTCATC CTGGACAACA AGTACACGGA GCACAGCACC 
GTGGGCCTCG CCCAGCAGTG GGACCAGCTG GACCAGCTGG GCATGCGCAT GCAGCACAAC 
CTGGAGCAGC AGATCCAGGC CAGGAACACA ACAGGTGTGA CTGAGGAGGC CCTCAAAGAA 
TTCAGCATGA TGTTTAAACA CTTTGACAAG GACAAGTCTG GCAGGCTGAA CCATCAGGAG 
TTCAAATCTT GCCTGCGCTC CCTGGGCTAT GACCTGCCCA TGGTGGAGGA AGGGGAACCT 
GACCCTGAGT TCGAGGCAAT CCTGGACACG GTGGATCCGA ACAGAGATGG CCATGTCTCC 
CTGCAAGAAT ACATGGCTTT CATGATCAGC CGCGAAACTG AGAACGTCAA GTCCAGCGAG 
GAGATTGAGA GCGCCTTCCG GGCCCTCAGC TCAGAGGGAA AGCCTTACGT GACCAAGGAG 
GAGCTCTACC AGAACCTGAC CCGGGAACAA GCCGACTACT GCGTCTCCCA CATGAAGCCC 
TACGTGGACG GCAAGGGCCG CGAGCTCCCC ACCGCGTTCG ACTACGTGGA GTTCACCCGC 
TCGCTTTTCG TGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009455693.2
CDS	121..7638
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRITKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_009457418.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009457418.2

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAATTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAATG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA ACACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCCC TGCTAGAGGC AGATGTGGCT 
GCTCATCAGG ACCGAATTGA TGGCATTACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATGTTGAGGA TGAGGAGACG TGGATTCGAG AGAAGGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCAGCT GCTTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AGACACGCAT AACTAAGGAG GCCGGCAGTG TATCTCTGCG TATGAAGCAG 
GTGGAAGAAC TATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAGGC TCTAAACACA GACAATTATG GACATGATCT TGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAAT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCGTTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAAATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACGGCAAG TGATGAGTCA TACAAGGATC CCACCAACAT CCAGAGCAAG 
CACCAGAAGC ACCAGGCTTT TGAAGCAGAG CTGCATGCCA ACGCTGACCG GATCCGTGGG 
GTTATCGACA TGGGCAACTC CCTCATTGAA CGTGGAGCCT GTGCCGGCAG TGAGGATGCT 
GTCAAGGCCC GCCTGGCTGC CTTAGCTGAC CAGTGGCAAT TCTTGGTGCA AAAGTCAGCG 
GAAAAGAGCC AGAAACTGAA AGAAGCCAAC AAGCAGCAGA ACTTCAACAC AGGGATCAAG 
GACTTTGACT TCTGGCTGTC TGAGGTGGAG GCCCTGCTGG CATCCGAAGA TTATGGCAAA 
GACCTGGCTT CTGTGAACAA CCTGCTGAAA AAGCATCAAC TGCTGGAAGC AGATATATCT 
GCCCATGAGG ATCGCCTGAA GGACCTGAAC AGCCAGGCAG ACAGCCTGAT GACCAGCAGT 
GCCTTCGACA CCTCCCAAGT AAAGGACAAG AGGGACACCA TCAACGGGCG CTTCCAGAAG 
ATCAAGAGCA TGGCGGCCTC CCGGCGAGCC AAGCTGAATG AATCCCATCG CCTGCACCAG 
TTCTTCCGGG ACATGGATGA CGAGGAGTCC TGGATCAAGG AGAAGAAGCT GCTGGTGGGC 
TCGGAGGACT ACGGCCGGGA CCTAACCGGC GTGCAGAACC TGAGGAAGAA GCACAAGCGC 
CTGGAAGCAG AACTGGCTGC GCATGAGCCG GCTATTCAGG GTGTCCTGGA CACTGGCAAG 
AAGCTGTCCG ATGATAACAC CATCGGGAAA GAGGAGATCC AGCAGCGGCT GGCGCAGTTT 
GTGGAGCACT GGAAAGAGCT GAAGCAGCTG GCAGCTGCCC GGGGTCAGCG GCTGGAAGAG 
TCCTTGGAAT ATCAGCAGTT TGTAGCCAAT GTGGAAGAGG AGGAAGCCTG GATCAATGAG 
AAAATGACCC TGGTGGCCAG CGAAGATTAT GGCGACACTC TTGCTGCCAT CCAGGGCTTA 
CTGAAGAAAC ATGAAGCTTT TGAGACAGAC TTCACCGTCC ACAAGGATCG CGTGAATGAC 
GTCTGCACCA ATGGACAAGA CCTCATTAAG AAGAACAATC ACCATGAGGA GAACATCTCT 
TCAAAGATGA AGGGCCTGAA CGGGAAAGTG TCAGACCTGG AGAAAGCTGC AGCCCAGAGA 
AAGGCGAAGC TGGATGAGAA CTCGGCCTTC CTTCAGTTCA ACTGGAAGGC GGACGTGGTG 
GAGTCCTGGA TCGGTGAAAA GGAGAACAGC TTGAAGACAG ATGATTATGG CCGAGACCTG 
TCTTCTGTGC AGACGCTCCT CACCAAACAG GAAACTTTTG ACGCTGGGCT GCAGGCCTTC 
CAGCAGGAAG GCATTGCCAA CATCACTGCC CTCAAAGATC AGCTTCTCGC CGCCAAACAC 
GTTCAGTCCA AGGCCATCGA GGCCCGGCAC GCCTCCCTCA TGAAGAGGTG GAGCCAGCTT 
CTGGCCAACT CAGCCGCCCG CAAGAAGAAG CTTCTGGAGG CGCAGAGTCA CTTCCGCAAG 
GTGGAGGACC TCTTCCTGAC CTTTGCCAAA AAGGCTTCTG CCTTCAACAG CTGGTTTGAA 
AATGCAGAGG AGGACTTAAC AGACCCCGTG CGCTGCAACT CCTTGGAAGA AATCAAAGCT 
TTGCGCGAGG CCCACGACGC CTTCCGCTCC TCCCTCAGCT CTGCCCAGGC TGACTTCAAC 
CAGCTGGCCG AGCTGGACCG CCAGATCAAG AGCTTCCGCG TAGCCTCCAA CCCCTACACC 
TGGTTTACCA TGGAGGCCCT GGAGGAGACC TGGAGGAACC TACAGAAAAT CATCAAGGAG 
AGGGAGCTGG AGCTGCAGAA GGAACAGCGG CGGCAGGAGG AGAACGACAA GCTGCGCCAG 
GAGTTTGCCC AGCACGCCAA CGCCTTCCAC CAGTGGATCC AAGAGACCAG GACATACCTC 
CTCGATGGCA TAGCATATCG TCGGGTCATT CGTGTCTATC AGTATGAAGT TGGGGATGAT 
CTGTCTGGAA GGTCCTGTAT GGTGGAAGAG TCGGGGACCC TCGAATCCCA GCTTGAAGCT 
ACCAAACGCA AGCACCAGGA AATCCGAGCC ATGAGAAGTC AGCTCAAAAA GATCGAGGAC 
CTGGGGGCCG CCATGGAGGA GGCCCTCATC CTGGACAACA AGTACACGGA GCACAGCACC 
GTGGGCCTCG CCCAGCAGTG GGACCAGCTG GACCAGCTGG GCATGCGCAT GCAGCACAAC 
CTGGAGCAGC AGATCCAGGC CAGGAACACA ACAGGTGTGA CTGAGGAGGC CCTCAAAGAA 
TTCAGCATGA TGTTTAAACA CTTTGACAAG GACAAGTCTG GCAGGCTGAA CCATCAGGAG 
TTCAAATCTT GCCTGCGCTC CCTGGGCTAT GACCTGCCCA TGGTGGAGGA AGGGGAACCT 
GACCCTGAGT TCGAGGCAAT CCTGGACACG GTGGATCCGA ACAGAGATGG CCATGTCTCC 
CTGCAAGAAT ACATGGCTTT CATGATCAGC CGCGAAACTG AGAACGTCAA GTCCAGCGAG 
GAGATTGAGA GCGCCTTCCG GGCCCTCAGC TCAGAGGGAA AGCCTTACGT GACCAAGGAG 
GAGCTCTACC AGAACCTGAC CCGGGAACAA GCCGACTACT GCGTCTCCCA CATGAAGCCC 
TACGTGGACG GCAAGGGCCG CGAGCTCCCC ACCGCGTTCG ACTACGTGGA GTTCACCCGC 
TCGCTTTTCG TGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_009457419.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009455694.2 Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X2, mRNA.

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CloneID	OPc89630
Clone ID Related Accession (Same CDS sequence)	XM_009457419.2
Accession Version	XM_009457419.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7497bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006476.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009457419.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAA TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AATGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAACACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCCCTGCTAG AGGCAGATGT GGCTGCTCAT CAGGACCGAA TTGATGGCAT TACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAGG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC AGCTGCTTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAGACAC GCATAACTAA GGAGGCCGGC 
AGTGTATCTC TGCGTATGAA GCAGGTGGAA GAACTATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AGGCTCTAAA CACAGACAAT 
TATGGACATG ATCTTGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAATCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCGTT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAAATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACGG CAAGTGATGA GTCATACAAG 
GATCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAGGCTTTT 
GAAGCAGAGC TGCATGCCAA CGCTGACCGG ATCCGTGGGG TTATCGACAT GGGCAACTCC 
CTCATTGAAC GTGGAGCCTG TGCCGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
TTAGCTGACC AGTGGCAATT CTTGGTGCAA AAGTCAGCGG AAAAGAGCCA GAAACTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACA GGGATCAAGG ACTTTGACTT CTGGCTGTCT 
GAGGTGGAGG CCCTGCTGGC ATCCGAAGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC 
CTGCTGAAAA AGCATCAACT GCTGGAAGCA GATATATCTG CCCATGAGGA TCGCCTGAAG 
GACCTGAACA GCCAGGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTA 
AAGGACAAGA GGGACACCAT CAACGGGCGC TTCCAGAAGA TCAAGAGCAT GGCGGCCTCC 
CGGCGAGCCA AGCTGAATGA ATCCCATCGC CTGCACCAGT TCTTCCGGGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGGGCT CGGAGGACTA CGGCCGGGAC 
CTAACCGGCG TGCAGAACCT GAGGAAGAAG CACAAGCGCC TGGAAGCAGA ACTGGCTGCG 
CATGAGCCGG CTATTCAGGG TGTCCTGGAC ACTGGCAAGA AGCTGTCCGA TGATAACACC 
ATCGGGAAAG AGGAGATCCA GCAGCGGCTG GCGCAGTTTG TGGAGCACTG GAAAGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGTCAGCGG CTGGAAGAGT CCTTGGAATA TCAGCAGTTT 
GTAGCCAATG TGGAAGAGGA GGAAGCCTGG ATCAATGAGA AAATGACCCT GGTGGCCAGC 
GAAGATTATG GCGACACTCT TGCTGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACCGTCCA CAAGGATCGC GTGAATGACG TCTGCACCAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGCCTGAAC 
GGGAAAGTGT CAGACCTGGA GAAAGCTGCA GCCCAGAGAA AGGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA CTGGAAGGCG GACGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGC CGAGACCTGT CTTCTGTGCA GACGCTCCTC 
ACCAAACAGG AAACTTTTGA CGCTGGGCTG CAGGCCTTCC AGCAGGAAGG CATTGCCAAC 
ATCACTGCCC TCAAAGATCA GCTTCTCGCC GCCAAACACG TTCAGTCCAA GGCCATCGAG 
GCCCGGCACG CCTCCCTCAT GAAGAGGTGG AGCCAGCTTC TGGCCAACTC AGCCGCCCGC 
AAGAAGAAGC TTCTGGAGGC GCAGAGTCAC TTCCGCAAGG TGGAGGACCT CTTCCTGACC 
TTTGCCAAAA AGGCTTCTGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCCGTGC GCTGCAACTC CTTGGAAGAA ATCAAAGCTT TGCGCGAGGC CCACGACGCC 
TTCCGCTCCT CCCTCAGCTC TGCCCAGGCT GACTTCAACC AGCTGGCCGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT AGCCTCCAAC CCCTACACCT GGTTTACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT ACAGAAAATC ATCAAGGAGA GGGAGCTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTTGCCCA GCACGCCAAC 
GCCTTCCACC AGTGGATCCA AGAGACCAGG ACATACCTCC TCGATGGCAT AGCATATCGT 
CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGTATG 
GTGGAAGAGT CGGGGACCCT CGAATCCCAG CTTGAAGCTA CCAAACGCAA GCACCAGGAA 
ATCCGAGCCA TGAGAAGTCA GCTCAAAAAG ATCGAGGACC TGGGGGCCGC CATGGAGGAG 
GCCCTCATCC TGGACAACAA GTACACGGAG CACAGCACCG TGGGCCTCGC CCAGCAGTGG 
GACCAGCTGG ACCAGCTGGG CATGCGCATG CAGCACAACC TGGAGCAGCA GATCCAGGCC 
AGGAACACAA CAGGTGTGAC TGAGGAGGCC CTCAAAGAAT TCAGCATGAT GTTTAAACAC 
TTTGACAAGG ACAAGTCTGG CAGGCTGAAC CATCAGGAGT TCAAATCTTG CCTGCGCTCC 
CTGGGCTATG ACCTGCCCAT GGTGGAGGAA GGGGAACCTG ACCCTGAGTT CGAGGCAATC 
CTGGACACGG TGGATCCGAA CAGAGATGGC CATGTCTCCC TGCAAGAATA CATGGCTTTC 
ATGATCAGCC GCGAAACTGA GAACGTCAAG TCCAGCGAGG AGATTGAGAG CGCCTTCCGG 
GCCCTCAGCT CAGAGGGAAA GCCTTACGTG ACCAAGGAGG AGCTCTACCA GAACCTGACC 
CGGGAACAAG CCGACTACTG CGTCTCCCAC ATGAAGCCCT ACGTGGACGG CAAGGGCCGC 
GAGCTCCCCA CCGCGTTCGA CTACGTGGAG TTCACCCGCT CGCTTTTCGT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009455694.2
CDS	138..7634
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRITKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_009457419.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009457419.2

ORF Insert Sequence:

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAA TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AATGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAACACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCCCTGCTAG AGGCAGATGT GGCTGCTCAT CAGGACCGAA TTGATGGCAT TACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAGG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC AGCTGCTTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAGACAC GCATAACTAA GGAGGCCGGC 
AGTGTATCTC TGCGTATGAA GCAGGTGGAA GAACTATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AGGCTCTAAA CACAGACAAT 
TATGGACATG ATCTTGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAATCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCGTT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAAATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACGG CAAGTGATGA GTCATACAAG 
GATCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAGGCTTTT 
GAAGCAGAGC TGCATGCCAA CGCTGACCGG ATCCGTGGGG TTATCGACAT GGGCAACTCC 
CTCATTGAAC GTGGAGCCTG TGCCGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
TTAGCTGACC AGTGGCAATT CTTGGTGCAA AAGTCAGCGG AAAAGAGCCA GAAACTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACA GGGATCAAGG ACTTTGACTT CTGGCTGTCT 
GAGGTGGAGG CCCTGCTGGC ATCCGAAGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC 
CTGCTGAAAA AGCATCAACT GCTGGAAGCA GATATATCTG CCCATGAGGA TCGCCTGAAG 
GACCTGAACA GCCAGGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTA 
AAGGACAAGA GGGACACCAT CAACGGGCGC TTCCAGAAGA TCAAGAGCAT GGCGGCCTCC 
CGGCGAGCCA AGCTGAATGA ATCCCATCGC CTGCACCAGT TCTTCCGGGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGGGCT CGGAGGACTA CGGCCGGGAC 
CTAACCGGCG TGCAGAACCT GAGGAAGAAG CACAAGCGCC TGGAAGCAGA ACTGGCTGCG 
CATGAGCCGG CTATTCAGGG TGTCCTGGAC ACTGGCAAGA AGCTGTCCGA TGATAACACC 
ATCGGGAAAG AGGAGATCCA GCAGCGGCTG GCGCAGTTTG TGGAGCACTG GAAAGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGTCAGCGG CTGGAAGAGT CCTTGGAATA TCAGCAGTTT 
GTAGCCAATG TGGAAGAGGA GGAAGCCTGG ATCAATGAGA AAATGACCCT GGTGGCCAGC 
GAAGATTATG GCGACACTCT TGCTGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACCGTCCA CAAGGATCGC GTGAATGACG TCTGCACCAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGCCTGAAC 
GGGAAAGTGT CAGACCTGGA GAAAGCTGCA GCCCAGAGAA AGGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA CTGGAAGGCG GACGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGC CGAGACCTGT CTTCTGTGCA GACGCTCCTC 
ACCAAACAGG AAACTTTTGA CGCTGGGCTG CAGGCCTTCC AGCAGGAAGG CATTGCCAAC 
ATCACTGCCC TCAAAGATCA GCTTCTCGCC GCCAAACACG TTCAGTCCAA GGCCATCGAG 
GCCCGGCACG CCTCCCTCAT GAAGAGGTGG AGCCAGCTTC TGGCCAACTC AGCCGCCCGC 
AAGAAGAAGC TTCTGGAGGC GCAGAGTCAC TTCCGCAAGG TGGAGGACCT CTTCCTGACC 
TTTGCCAAAA AGGCTTCTGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCCGTGC GCTGCAACTC CTTGGAAGAA ATCAAAGCTT TGCGCGAGGC CCACGACGCC 
TTCCGCTCCT CCCTCAGCTC TGCCCAGGCT GACTTCAACC AGCTGGCCGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT AGCCTCCAAC CCCTACACCT GGTTTACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT ACAGAAAATC ATCAAGGAGA GGGAGCTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTTGCCCA GCACGCCAAC 
GCCTTCCACC AGTGGATCCA AGAGACCAGG ACATACCTCC TCGATGGCAT AGCATATCGT 
CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGTATG 
GTGGAAGAGT CGGGGACCCT CGAATCCCAG CTTGAAGCTA CCAAACGCAA GCACCAGGAA 
ATCCGAGCCA TGAGAAGTCA GCTCAAAAAG ATCGAGGACC TGGGGGCCGC CATGGAGGAG 
GCCCTCATCC TGGACAACAA GTACACGGAG CACAGCACCG TGGGCCTCGC CCAGCAGTGG 
GACCAGCTGG ACCAGCTGGG CATGCGCATG CAGCACAACC TGGAGCAGCA GATCCAGGCC 
AGGAACACAA CAGGTGTGAC TGAGGAGGCC CTCAAAGAAT TCAGCATGAT GTTTAAACAC 
TTTGACAAGG ACAAGTCTGG CAGGCTGAAC CATCAGGAGT TCAAATCTTG CCTGCGCTCC 
CTGGGCTATG ACCTGCCCAT GGTGGAGGAA GGGGAACCTG ACCCTGAGTT CGAGGCAATC 
CTGGACACGG TGGATCCGAA CAGAGATGGC CATGTCTCCC TGCAAGAATA CATGGCTTTC 
ATGATCAGCC GCGAAACTGA GAACGTCAAG TCCAGCGAGG AGATTGAGAG CGCCTTCCGG 
GCCCTCAGCT CAGAGGGAAA GCCTTACGTG ACCAAGGAGG AGCTCTACCA GAACCTGACC 
CGGGAACAAG CCGACTACTG CGTCTCCCAC ATGAAGCCCT ACGTGGACGG CAAGGGCCGC 
GAGCTCCCCA CCGCGTTCGA CTACGTGGAG TTCACCCGCT CGCTTTTCGT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_016961795.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016817284.1 Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X3, mRNA.

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CloneID	OPc89631
Clone ID Related Accession (Same CDS sequence)	XM_016961795.1
Accession Version	XM_016961795.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7473bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006476.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAATTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAATG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA ACACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCCC TGCTAGAGGC AGATGTGGCT 
GCTCATCAGG ACCGAATTGA TGGCATTACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATGTTGAGGA TGAGGAGACG TGGATTCGAG AGAAGGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCAGCT GCTTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAGGC TCTAAACACA GACAATTATG GACATGATCT TGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAAT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCGTTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAAATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACGGCAAG TGATGAGTCA TACAAGGATC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCACCA GAAGCACCAG GCTTTTGAAG CAGAGCTGCA TGCCAACGCT 
GACCGGATCC GTGGGGTTAT CGACATGGGC AACTCCCTCA TTGAACGTGG AGCCTGTGCC 
GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCTTAG CTGACCAGTG GCAATTCTTG 
GTGCAAAAGT CAGCGGAAAA GAGCCAGAAA CTGAAAGAAG CCAACAAGCA GCAGAACTTC 
AACACAGGGA TCAAGGACTT TGACTTCTGG CTGTCTGAGG TGGAGGCCCT GCTGGCATCC 
GAAGATTATG GCAAAGACCT GGCTTCTGTG AACAACCTGC TGAAAAAGCA TCAACTGCTG 
GAAGCAGATA TATCTGCCCA TGAGGATCGC CTGAAGGACC TGAACAGCCA GGCAGACAGC 
CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTAAAGG ACAAGAGGGA CACCATCAAC 
GGGCGCTTCC AGAAGATCAA GAGCATGGCG GCCTCCCGGC GAGCCAAGCT GAATGAATCC 
CATCGCCTGC ACCAGTTCTT CCGGGACATG GATGACGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGGGCTCGGA GGACTACGGC CGGGACCTAA CCGGCGTGCA GAACCTGAGG 
AAGAAGCACA AGCGCCTGGA AGCAGAACTG GCTGCGCATG AGCCGGCTAT TCAGGGTGTC 
CTGGACACTG GCAAGAAGCT GTCCGATGAT AACACCATCG GGAAAGAGGA GATCCAGCAG 
CGGCTGGCGC AGTTTGTGGA GCACTGGAAA GAGCTGAAGC AGCTGGCAGC TGCCCGGGGT 
CAGCGGCTGG AAGAGTCCTT GGAATATCAG CAGTTTGTAG CCAATGTGGA AGAGGAGGAA 
GCCTGGATCA ATGAGAAAAT GACCCTGGTG GCCAGCGAAG ATTATGGCGA CACTCTTGCT 
GCCATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC CGTCCACAAG 
GATCGCGTGA ATGACGTCTG CACCAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCAAA GATGAAGGGC CTGAACGGGA AAGTGTCAGA CCTGGAGAAA 
GCTGCAGCCC AGAGAAAGGC GAAGCTGGAT GAGAACTCGG CCTTCCTTCA GTTCAACTGG 
AAGGCGGACG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGCCGAG ACCTGTCTTC TGTGCAGACG CTCCTCACCA AACAGGAAAC TTTTGACGCT 
GGGCTGCAGG CCTTCCAGCA GGAAGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTT 
CTCGCCGCCA AACACGTTCA GTCCAAGGCC ATCGAGGCCC GGCACGCCTC CCTCATGAAG 
AGGTGGAGCC AGCTTCTGGC CAACTCAGCC GCCCGCAAGA AGAAGCTTCT GGAGGCGCAG 
AGTCACTTCC GCAAGGTGGA GGACCTCTTC CTGACCTTTG CCAAAAAGGC TTCTGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CCGTGCGCTG CAACTCCTTG 
GAAGAAATCA AAGCTTTGCG CGAGGCCCAC GACGCCTTCC GCTCCTCCCT CAGCTCTGCC 
CAGGCTGACT TCAACCAGCT GGCCGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTAGCC 
TCCAACCCCT ACACCTGGTT TACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTACAG 
AAAATCATCA AGGAGAGGGA GCTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT TGCCCAGCAC GCCAACGCCT TCCACCAGTG GATCCAAGAG 
ACCAGGACAT ACCTCCTCGA TGGCATAGCA TATCGTCGGG TCATTCGTGT CTATCAGTAT 
GAAGTTGGGG ATGATCTGTC TGGAAGGTCC TGTATGGTGG AAGAGTCGGG GACCCTCGAA 
TCCCAGCTTG AAGCTACCAA ACGCAAGCAC CAGGAAATCC GAGCCATGAG AAGTCAGCTC 
AAAAAGATCG AGGACCTGGG GGCCGCCATG GAGGAGGCCC TCATCCTGGA CAACAAGTAC 
ACGGAGCACA GCACCGTGGG CCTCGCCCAG CAGTGGGACC AGCTGGACCA GCTGGGCATG 
CGCATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACAACAGG TGTGACTGAG 
GAGGCCCTCA AAGAATTCAG CATGATGTTT AAACACTTTG ACAAGGACAA GTCTGGCAGG 
CTGAACCATC AGGAGTTCAA ATCTTGCCTG CGCTCCCTGG GCTATGACCT GCCCATGGTG 
GAGGAAGGGG AACCTGACCC TGAGTTCGAG GCAATCCTGG ACACGGTGGA TCCGAACAGA 
GATGGCCATG TCTCCCTGCA AGAATACATG GCTTTCATGA TCAGCCGCGA AACTGAGAAC 
GTCAAGTCCA GCGAGGAGAT TGAGAGCGCC TTCCGGGCCC TCAGCTCAGA GGGAAAGCCT 
TACGTGACCA AGGAGGAGCT CTACCAGAAC CTGACCCGGG AACAAGCCGA CTACTGCGTC 
TCCCACATGA AGCCCTACGT GGACGGCAAG GGCCGCGAGC TCCCCACCGC GTTCGACTAC 
GTGGAGTTCA CCCGCTCGCT TTTCGTGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016817284.1
CDS	121..7593
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_016961795.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016961795.1

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAATTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAATG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA ACACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCCC TGCTAGAGGC AGATGTGGCT 
GCTCATCAGG ACCGAATTGA TGGCATTACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATGTTGAGGA TGAGGAGACG TGGATTCGAG AGAAGGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCAGCT GCTTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAGGC TCTAAACACA GACAATTATG GACATGATCT TGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAAT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCGTTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAAATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACGGCAAG TGATGAGTCA TACAAGGATC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCACCA GAAGCACCAG GCTTTTGAAG CAGAGCTGCA TGCCAACGCT 
GACCGGATCC GTGGGGTTAT CGACATGGGC AACTCCCTCA TTGAACGTGG AGCCTGTGCC 
GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCTTAG CTGACCAGTG GCAATTCTTG 
GTGCAAAAGT CAGCGGAAAA GAGCCAGAAA CTGAAAGAAG CCAACAAGCA GCAGAACTTC 
AACACAGGGA TCAAGGACTT TGACTTCTGG CTGTCTGAGG TGGAGGCCCT GCTGGCATCC 
GAAGATTATG GCAAAGACCT GGCTTCTGTG AACAACCTGC TGAAAAAGCA TCAACTGCTG 
GAAGCAGATA TATCTGCCCA TGAGGATCGC CTGAAGGACC TGAACAGCCA GGCAGACAGC 
CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTAAAGG ACAAGAGGGA CACCATCAAC 
GGGCGCTTCC AGAAGATCAA GAGCATGGCG GCCTCCCGGC GAGCCAAGCT GAATGAATCC 
CATCGCCTGC ACCAGTTCTT CCGGGACATG GATGACGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGGGCTCGGA GGACTACGGC CGGGACCTAA CCGGCGTGCA GAACCTGAGG 
AAGAAGCACA AGCGCCTGGA AGCAGAACTG GCTGCGCATG AGCCGGCTAT TCAGGGTGTC 
CTGGACACTG GCAAGAAGCT GTCCGATGAT AACACCATCG GGAAAGAGGA GATCCAGCAG 
CGGCTGGCGC AGTTTGTGGA GCACTGGAAA GAGCTGAAGC AGCTGGCAGC TGCCCGGGGT 
CAGCGGCTGG AAGAGTCCTT GGAATATCAG CAGTTTGTAG CCAATGTGGA AGAGGAGGAA 
GCCTGGATCA ATGAGAAAAT GACCCTGGTG GCCAGCGAAG ATTATGGCGA CACTCTTGCT 
GCCATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC CGTCCACAAG 
GATCGCGTGA ATGACGTCTG CACCAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCAAA GATGAAGGGC CTGAACGGGA AAGTGTCAGA CCTGGAGAAA 
GCTGCAGCCC AGAGAAAGGC GAAGCTGGAT GAGAACTCGG CCTTCCTTCA GTTCAACTGG 
AAGGCGGACG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGCCGAG ACCTGTCTTC TGTGCAGACG CTCCTCACCA AACAGGAAAC TTTTGACGCT 
GGGCTGCAGG CCTTCCAGCA GGAAGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTT 
CTCGCCGCCA AACACGTTCA GTCCAAGGCC ATCGAGGCCC GGCACGCCTC CCTCATGAAG 
AGGTGGAGCC AGCTTCTGGC CAACTCAGCC GCCCGCAAGA AGAAGCTTCT GGAGGCGCAG 
AGTCACTTCC GCAAGGTGGA GGACCTCTTC CTGACCTTTG CCAAAAAGGC TTCTGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CCGTGCGCTG CAACTCCTTG 
GAAGAAATCA AAGCTTTGCG CGAGGCCCAC GACGCCTTCC GCTCCTCCCT CAGCTCTGCC 
CAGGCTGACT TCAACCAGCT GGCCGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTAGCC 
TCCAACCCCT ACACCTGGTT TACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTACAG 
AAAATCATCA AGGAGAGGGA GCTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT TGCCCAGCAC GCCAACGCCT TCCACCAGTG GATCCAAGAG 
ACCAGGACAT ACCTCCTCGA TGGCATAGCA TATCGTCGGG TCATTCGTGT CTATCAGTAT 
GAAGTTGGGG ATGATCTGTC TGGAAGGTCC TGTATGGTGG AAGAGTCGGG GACCCTCGAA 
TCCCAGCTTG AAGCTACCAA ACGCAAGCAC CAGGAAATCC GAGCCATGAG AAGTCAGCTC 
AAAAAGATCG AGGACCTGGG GGCCGCCATG GAGGAGGCCC TCATCCTGGA CAACAAGTAC 
ACGGAGCACA GCACCGTGGG CCTCGCCCAG CAGTGGGACC AGCTGGACCA GCTGGGCATG 
CGCATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACAACAGG TGTGACTGAG 
GAGGCCCTCA AAGAATTCAG CATGATGTTT AAACACTTTG ACAAGGACAA GTCTGGCAGG 
CTGAACCATC AGGAGTTCAA ATCTTGCCTG CGCTCCCTGG GCTATGACCT GCCCATGGTG 
GAGGAAGGGG AACCTGACCC TGAGTTCGAG GCAATCCTGG ACACGGTGGA TCCGAACAGA 
GATGGCCATG TCTCCCTGCA AGAATACATG GCTTTCATGA TCAGCCGCGA AACTGAGAAC 
GTCAAGTCCA GCGAGGAGAT TGAGAGCGCC TTCCGGGCCC TCAGCTCAGA GGGAAAGCCT 
TACGTGACCA AGGAGGAGCT CTACCAGAAC CTGACCCGGG AACAAGCCGA CTACTGCGTC 
TCCCACATGA AGCCCTACGT GGACGGCAAG GGCCGCGAGC TCCCCACCGC GTTCGACTAC 
GTGGAGTTCA CCCGCTCGCT TTTCGTGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_016961796.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016817285.1 Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X4, mRNA.

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CloneID	OPc89632
Clone ID Related Accession (Same CDS sequence)	XM_016961796.1
Accession Version	XM_016961796.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7470bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006476.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAATTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAATG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA ACACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCCC TGCTAGAGGC AGATGTGGCT 
GCTCATCAGG ACCGAATTGA TGGCATTACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATGTTGAGGA TGAGGAGACG TGGATTCGAG AGAAGGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCAGCT GCTTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AGACACGCAT AACTAAGGAG GCCGGCAGTG TATCTCTGCG TATGAAGCAG 
GTGGAAGAAC TATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAGGC TCTAAACACA GACAATTATG GACATGATCT TGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAAT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCGTTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAAATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACGGCAAG TGATGAGTCA TACAAGGATC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCACCA GAAGCACCAG GCTTTTGAAG CAGAGCTGCA TGCCAACGCT 
GACCGGATCC GTGGGGTTAT CGACATGGGC AACTCCCTCA TTGAACGTGG AGCCTGTGCC 
GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCTTAG CTGACCAGTG GCAATTCTTG 
GTGCAAAAGT CAGCGGAAAA GAGCCAGAAA CTGAAAGAAG CCAACAAGCA GCAGAACTTC 
AACACAGGGA TCAAGGACTT TGACTTCTGG CTGTCTGAGG TGGAGGCCCT GCTGGCATCC 
GAAGATTATG GCAAAGACCT GGCTTCTGTG AACAACCTGC TGAAAAAGCA TCAACTGCTG 
GAAGCAGATA TATCTGCCCA TGAGGATCGC CTGAAGGACC TGAACAGCCA GGCAGACAGC 
CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTAAAGG ACAAGAGGGA CACCATCAAC 
GGGCGCTTCC AGAAGATCAA GAGCATGGCG GCCTCCCGGC GAGCCAAGCT GAATGAATCC 
CATCGCCTGC ACCAGTTCTT CCGGGACATG GATGACGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGGGCTCGGA GGACTACGGC CGGGACCTAA CCGGCGTGCA GAACCTGAGG 
AAGAAGCACA AGCGCCTGGA AGCAGAACTG GCTGCGCATG AGCCGGCTAT TCAGGGTGTC 
CTGGACACTG GCAAGAAGCT GTCCGATGAT AACACCATCG GGAAAGAGGA GATCCAGCAG 
CGGCTGGCGC AGTTTGTGGA GCACTGGAAA GAGCTGAAGC AGCTGGCAGC TGCCCGGGGT 
CAGCGGCTGG AAGAGTCCTT GGAATATCAG CAGTTTGTAG CCAATGTGGA AGAGGAGGAA 
GCCTGGATCA ATGAGAAAAT GACCCTGGTG GCCAGCGAAG ATTATGGCGA CACTCTTGCT 
GCCATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC CGTCCACAAG 
GATCGCGTGA ATGACGTCTG CACCAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCAAA GATGAAGGGC CTGAACGGGA AAGTGTCAGA CCTGGAGAAA 
GCTGCAGCCC AGAGAAAGGC GAAGCTGGAT GAGAACTCGG CCTTCCTTCA GTTCAACTGG 
AAGGCGGACG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGCCGAG ACCTGTCTTC TGTGCAGACG CTCCTCACCA AACAGGAAAC TTTTGACGCT 
GGGCTGCAGG CCTTCCAGCA GGAAGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTT 
CTCGCCGCCA AACACGTTCA GTCCAAGGCC ATCGAGGCCC GGCACGCCTC CCTCATGAAG 
AGGTGGAGCC AGCTTCTGGC CAACTCAGCC GCCCGCAAGA AGAAGCTTCT GGAGGCGCAG 
AGTCACTTCC GCAAGGTGGA GGACCTCTTC CTGACCTTTG CCAAAAAGGC TTCTGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CCGTGCGCTG CAACTCCTTG 
GAAGAAATCA AAGCTTTGCG CGAGGCCCAC GACGCCTTCC GCTCCTCCCT CAGCTCTGCC 
CAGGCTGACT TCAACCAGCT GGCCGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTAGCC 
TCCAACCCCT ACACCTGGTT TACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTACAG 
AAAATCATCA AGGAGAGGGA GCTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT TGCCCAGCAC GCCAACGCCT TCCACCAGTG GATCCAAGAG 
ACCAGGACAT ACCTCCTCGA TGGGTCCTGT ATGGTGGAAG AGTCGGGGAC CCTCGAATCC 
CAGCTTGAAG CTACCAAACG CAAGCACCAG GAAATCCGAG CCATGAGAAG TCAGCTCAAA 
AAGATCGAGG ACCTGGGGGC CGCCATGGAG GAGGCCCTCA TCCTGGACAA CAAGTACACG 
GAGCACAGCA CCGTGGGCCT CGCCCAGCAG TGGGACCAGC TGGACCAGCT GGGCATGCGC 
ATGCAGCACA ACCTGGAGCA GCAGATCCAG GCCAGGAACA CAACAGGTGT GACTGAGGAG 
GCCCTCAAAG AATTCAGCAT GATGTTTAAA CACTTTGACA AGGACAAGTC TGGCAGGCTG 
AACCATCAGG AGTTCAAATC TTGCCTGCGC TCCCTGGGCT ATGACCTGCC CATGGTGGAG 
GAAGGGGAAC CTGACCCTGA GTTCGAGGCA ATCCTGGACA CGGTGGATCC GAACAGAGAT 
GGCCATGTCT CCCTGCAAGA ATACATGGCT TTCATGATCA GCCGCGAAAC TGAGAACGTC 
AAGTCCAGCG AGGAGATTGA GAGCGCCTTC CGGGCCCTCA GCTCAGAGGG AAAGCCTTAC 
GTGACCAAGG AGGAGCTCTA CCAGAACCTG ACCCGGGAAC AAGCCGACTA CTGCGTCTCC 
CACATGAAGC CCTACGTGGA CGGCAAGGGC CGCGAGCTCC CCACCGCGTT CGACTACGTG 
GAGTTCACCC GCTCGCTTTT CGTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016817285.1
CDS	121..7590
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRITKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_016961796.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016961796.1

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAATTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAATG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA ACACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCCC TGCTAGAGGC AGATGTGGCT 
GCTCATCAGG ACCGAATTGA TGGCATTACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATGTTGAGGA TGAGGAGACG TGGATTCGAG AGAAGGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCAGCT GCTTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AGACACGCAT AACTAAGGAG GCCGGCAGTG TATCTCTGCG TATGAAGCAG 
GTGGAAGAAC TATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAGGC TCTAAACACA GACAATTATG GACATGATCT TGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAAT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCGTTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAAATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACGGCAAG TGATGAGTCA TACAAGGATC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCACCA GAAGCACCAG GCTTTTGAAG CAGAGCTGCA TGCCAACGCT 
GACCGGATCC GTGGGGTTAT CGACATGGGC AACTCCCTCA TTGAACGTGG AGCCTGTGCC 
GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCTTAG CTGACCAGTG GCAATTCTTG 
GTGCAAAAGT CAGCGGAAAA GAGCCAGAAA CTGAAAGAAG CCAACAAGCA GCAGAACTTC 
AACACAGGGA TCAAGGACTT TGACTTCTGG CTGTCTGAGG TGGAGGCCCT GCTGGCATCC 
GAAGATTATG GCAAAGACCT GGCTTCTGTG AACAACCTGC TGAAAAAGCA TCAACTGCTG 
GAAGCAGATA TATCTGCCCA TGAGGATCGC CTGAAGGACC TGAACAGCCA GGCAGACAGC 
CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTAAAGG ACAAGAGGGA CACCATCAAC 
GGGCGCTTCC AGAAGATCAA GAGCATGGCG GCCTCCCGGC GAGCCAAGCT GAATGAATCC 
CATCGCCTGC ACCAGTTCTT CCGGGACATG GATGACGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGGGCTCGGA GGACTACGGC CGGGACCTAA CCGGCGTGCA GAACCTGAGG 
AAGAAGCACA AGCGCCTGGA AGCAGAACTG GCTGCGCATG AGCCGGCTAT TCAGGGTGTC 
CTGGACACTG GCAAGAAGCT GTCCGATGAT AACACCATCG GGAAAGAGGA GATCCAGCAG 
CGGCTGGCGC AGTTTGTGGA GCACTGGAAA GAGCTGAAGC AGCTGGCAGC TGCCCGGGGT 
CAGCGGCTGG AAGAGTCCTT GGAATATCAG CAGTTTGTAG CCAATGTGGA AGAGGAGGAA 
GCCTGGATCA ATGAGAAAAT GACCCTGGTG GCCAGCGAAG ATTATGGCGA CACTCTTGCT 
GCCATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC CGTCCACAAG 
GATCGCGTGA ATGACGTCTG CACCAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCAAA GATGAAGGGC CTGAACGGGA AAGTGTCAGA CCTGGAGAAA 
GCTGCAGCCC AGAGAAAGGC GAAGCTGGAT GAGAACTCGG CCTTCCTTCA GTTCAACTGG 
AAGGCGGACG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGCCGAG ACCTGTCTTC TGTGCAGACG CTCCTCACCA AACAGGAAAC TTTTGACGCT 
GGGCTGCAGG CCTTCCAGCA GGAAGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTT 
CTCGCCGCCA AACACGTTCA GTCCAAGGCC ATCGAGGCCC GGCACGCCTC CCTCATGAAG 
AGGTGGAGCC AGCTTCTGGC CAACTCAGCC GCCCGCAAGA AGAAGCTTCT GGAGGCGCAG 
AGTCACTTCC GCAAGGTGGA GGACCTCTTC CTGACCTTTG CCAAAAAGGC TTCTGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CCGTGCGCTG CAACTCCTTG 
GAAGAAATCA AAGCTTTGCG CGAGGCCCAC GACGCCTTCC GCTCCTCCCT CAGCTCTGCC 
CAGGCTGACT TCAACCAGCT GGCCGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTAGCC 
TCCAACCCCT ACACCTGGTT TACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTACAG 
AAAATCATCA AGGAGAGGGA GCTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT TGCCCAGCAC GCCAACGCCT TCCACCAGTG GATCCAAGAG 
ACCAGGACAT ACCTCCTCGA TGGGTCCTGT ATGGTGGAAG AGTCGGGGAC CCTCGAATCC 
CAGCTTGAAG CTACCAAACG CAAGCACCAG GAAATCCGAG CCATGAGAAG TCAGCTCAAA 
AAGATCGAGG ACCTGGGGGC CGCCATGGAG GAGGCCCTCA TCCTGGACAA CAAGTACACG 
GAGCACAGCA CCGTGGGCCT CGCCCAGCAG TGGGACCAGC TGGACCAGCT GGGCATGCGC 
ATGCAGCACA ACCTGGAGCA GCAGATCCAG GCCAGGAACA CAACAGGTGT GACTGAGGAG 
GCCCTCAAAG AATTCAGCAT GATGTTTAAA CACTTTGACA AGGACAAGTC TGGCAGGCTG 
AACCATCAGG AGTTCAAATC TTGCCTGCGC TCCCTGGGCT ATGACCTGCC CATGGTGGAG 
GAAGGGGAAC CTGACCCTGA GTTCGAGGCA ATCCTGGACA CGGTGGATCC GAACAGAGAT 
GGCCATGTCT CCCTGCAAGA ATACATGGCT TTCATGATCA GCCGCGAAAC TGAGAACGTC 
AAGTCCAGCG AGGAGATTGA GAGCGCCTTC CGGGCCCTCA GCTCAGAGGG AAAGCCTTAC 
GTGACCAAGG AGGAGCTCTA CCAGAACCTG ACCCGGGAAC AAGCCGACTA CTGCGTCTCC 
CACATGAAGC CCTACGTGGA CGGCAAGGGC CGCGAGCTCC CCACCGCGTT CGACTACGTG 
GAGTTCACCC GCTCGCTTTT CGTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_016961797.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016817286.1 Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X5, mRNA.

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CloneID	OPc89633
Clone ID Related Accession (Same CDS sequence)	XM_016961797.1
Accession Version	XM_016961797.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7455bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006476.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAATTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAATG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA ACACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCCC TGCTAGAGGC AGATGTGGCT 
GCTCATCAGG ACCGAATTGA TGGCATTACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATGTTGAGGA TGAGGAGACG TGGATTCGAG AGAAGGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCAGCT GCTTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AGACACGCAT AACTAAGGAG GCCGGCAGTG TATCTCTGCG TATGAAGCAG 
GTGGAAGAAC TATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAGGC TCTAAACACA GACAATTATG GACATGATCT TGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAAT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCGTTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAAATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACGGCAAG TGATGAGTCA TACAAGGATC CCACCAACAT CCAGAGCAAG 
CACCAGAAGC ACCAGGCTTT TGAAGCAGAG CTGCATGCCA ACGCTGACCG GATCCGTGGG 
GTTATCGACA TGGGCAACTC CCTCATTGAA CGTGGAGCCT GTGCCGGCAG TGAGGATGCT 
GTCAAGGCCC GCCTGGCTGC CTTAGCTGAC CAGTGGCAAT TCTTGGTGCA AAAGTCAGCG 
GAAAAGAGCC AGAAACTGAA AGAAGCCAAC AAGCAGCAGA ACTTCAACAC AGGGATCAAG 
GACTTTGACT TCTGGCTGTC TGAGGTGGAG GCCCTGCTGG CATCCGAAGA TTATGGCAAA 
GACCTGGCTT CTGTGAACAA CCTGCTGAAA AAGCATCAAC TGCTGGAAGC AGATATATCT 
GCCCATGAGG ATCGCCTGAA GGACCTGAAC AGCCAGGCAG ACAGCCTGAT GACCAGCAGT 
GCCTTCGACA CCTCCCAAGT AAAGGACAAG AGGGACACCA TCAACGGGCG CTTCCAGAAG 
ATCAAGAGCA TGGCGGCCTC CCGGCGAGCC AAGCTGAATG AATCCCATCG CCTGCACCAG 
TTCTTCCGGG ACATGGATGA CGAGGAGTCC TGGATCAAGG AGAAGAAGCT GCTGGTGGGC 
TCGGAGGACT ACGGCCGGGA CCTAACCGGC GTGCAGAACC TGAGGAAGAA GCACAAGCGC 
CTGGAAGCAG AACTGGCTGC GCATGAGCCG GCTATTCAGG GTGTCCTGGA CACTGGCAAG 
AAGCTGTCCG ATGATAACAC CATCGGGAAA GAGGAGATCC AGCAGCGGCT GGCGCAGTTT 
GTGGAGCACT GGAAAGAGCT GAAGCAGCTG GCAGCTGCCC GGGGTCAGCG GCTGGAAGAG 
TCCTTGGAAT ATCAGCAGTT TGTAGCCAAT GTGGAAGAGG AGGAAGCCTG GATCAATGAG 
AAAATGACCC TGGTGGCCAG CGAAGATTAT GGCGACACTC TTGCTGCCAT CCAGGGCTTA 
CTGAAGAAAC ATGAAGCTTT TGAGACAGAC TTCACCGTCC ACAAGGATCG CGTGAATGAC 
GTCTGCACCA ATGGACAAGA CCTCATTAAG AAGAACAATC ACCATGAGGA GAACATCTCT 
TCAAAGATGA AGGGCCTGAA CGGGAAAGTG TCAGACCTGG AGAAAGCTGC AGCCCAGAGA 
AAGGCGAAGC TGGATGAGAA CTCGGCCTTC CTTCAGTTCA ACTGGAAGGC GGACGTGGTG 
GAGTCCTGGA TCGGTGAAAA GGAGAACAGC TTGAAGACAG ATGATTATGG CCGAGACCTG 
TCTTCTGTGC AGACGCTCCT CACCAAACAG GAAACTTTTG ACGCTGGGCT GCAGGCCTTC 
CAGCAGGAAG GCATTGCCAA CATCACTGCC CTCAAAGATC AGCTTCTCGC CGCCAAACAC 
GTTCAGTCCA AGGCCATCGA GGCCCGGCAC GCCTCCCTCA TGAAGAGGTG GAGCCAGCTT 
CTGGCCAACT CAGCCGCCCG CAAGAAGAAG CTTCTGGAGG CGCAGAGTCA CTTCCGCAAG 
GTGGAGGACC TCTTCCTGAC CTTTGCCAAA AAGGCTTCTG CCTTCAACAG CTGGTTTGAA 
AATGCAGAGG AGGACTTAAC AGACCCCGTG CGCTGCAACT CCTTGGAAGA AATCAAAGCT 
TTGCGCGAGG CCCACGACGC CTTCCGCTCC TCCCTCAGCT CTGCCCAGGC TGACTTCAAC 
CAGCTGGCCG AGCTGGACCG CCAGATCAAG AGCTTCCGCG TAGCCTCCAA CCCCTACACC 
TGGTTTACCA TGGAGGCCCT GGAGGAGACC TGGAGGAACC TACAGAAAAT CATCAAGGAG 
AGGGAGCTGG AGCTGCAGAA GGAACAGCGG CGGCAGGAGG AGAACGACAA GCTGCGCCAG 
GAGTTTGCCC AGCACGCCAA CGCCTTCCAC CAGTGGATCC AAGAGACCAG GACATACCTC 
CTCGATGGGT CCTGTATGGT GGAAGAGTCG GGGACCCTCG AATCCCAGCT TGAAGCTACC 
AAACGCAAGC ACCAGGAAAT CCGAGCCATG AGAAGTCAGC TCAAAAAGAT CGAGGACCTG 
GGGGCCGCCA TGGAGGAGGC CCTCATCCTG GACAACAAGT ACACGGAGCA CAGCACCGTG 
GGCCTCGCCC AGCAGTGGGA CCAGCTGGAC CAGCTGGGCA TGCGCATGCA GCACAACCTG 
GAGCAGCAGA TCCAGGCCAG GAACACAACA GGTGTGACTG AGGAGGCCCT CAAAGAATTC 
AGCATGATGT TTAAACACTT TGACAAGGAC AAGTCTGGCA GGCTGAACCA TCAGGAGTTC 
AAATCTTGCC TGCGCTCCCT GGGCTATGAC CTGCCCATGG TGGAGGAAGG GGAACCTGAC 
CCTGAGTTCG AGGCAATCCT GGACACGGTG GATCCGAACA GAGATGGCCA TGTCTCCCTG 
CAAGAATACA TGGCTTTCAT GATCAGCCGC GAAACTGAGA ACGTCAAGTC CAGCGAGGAG 
ATTGAGAGCG CCTTCCGGGC CCTCAGCTCA GAGGGAAAGC CTTACGTGAC CAAGGAGGAG 
CTCTACCAGA ACCTGACCCG GGAACAAGCC GACTACTGCG TCTCCCACAT GAAGCCCTAC 
GTGGACGGCA AGGGCCGCGA GCTCCCCACC GCGTTCGACT ACGTGGAGTT CACCCGCTCG 
CTTTTCGTGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016817286.1
CDS	121..7575
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRITKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_016961797.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016961797.1

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAATTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAATG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA ACACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCCC TGCTAGAGGC AGATGTGGCT 
GCTCATCAGG ACCGAATTGA TGGCATTACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATGTTGAGGA TGAGGAGACG TGGATTCGAG AGAAGGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCAGCT GCTTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AGACACGCAT AACTAAGGAG GCCGGCAGTG TATCTCTGCG TATGAAGCAG 
GTGGAAGAAC TATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAGGC TCTAAACACA GACAATTATG GACATGATCT TGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAAT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCGTTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAAATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACGGCAAG TGATGAGTCA TACAAGGATC CCACCAACAT CCAGAGCAAG 
CACCAGAAGC ACCAGGCTTT TGAAGCAGAG CTGCATGCCA ACGCTGACCG GATCCGTGGG 
GTTATCGACA TGGGCAACTC CCTCATTGAA CGTGGAGCCT GTGCCGGCAG TGAGGATGCT 
GTCAAGGCCC GCCTGGCTGC CTTAGCTGAC CAGTGGCAAT TCTTGGTGCA AAAGTCAGCG 
GAAAAGAGCC AGAAACTGAA AGAAGCCAAC AAGCAGCAGA ACTTCAACAC AGGGATCAAG 
GACTTTGACT TCTGGCTGTC TGAGGTGGAG GCCCTGCTGG CATCCGAAGA TTATGGCAAA 
GACCTGGCTT CTGTGAACAA CCTGCTGAAA AAGCATCAAC TGCTGGAAGC AGATATATCT 
GCCCATGAGG ATCGCCTGAA GGACCTGAAC AGCCAGGCAG ACAGCCTGAT GACCAGCAGT 
GCCTTCGACA CCTCCCAAGT AAAGGACAAG AGGGACACCA TCAACGGGCG CTTCCAGAAG 
ATCAAGAGCA TGGCGGCCTC CCGGCGAGCC AAGCTGAATG AATCCCATCG CCTGCACCAG 
TTCTTCCGGG ACATGGATGA CGAGGAGTCC TGGATCAAGG AGAAGAAGCT GCTGGTGGGC 
TCGGAGGACT ACGGCCGGGA CCTAACCGGC GTGCAGAACC TGAGGAAGAA GCACAAGCGC 
CTGGAAGCAG AACTGGCTGC GCATGAGCCG GCTATTCAGG GTGTCCTGGA CACTGGCAAG 
AAGCTGTCCG ATGATAACAC CATCGGGAAA GAGGAGATCC AGCAGCGGCT GGCGCAGTTT 
GTGGAGCACT GGAAAGAGCT GAAGCAGCTG GCAGCTGCCC GGGGTCAGCG GCTGGAAGAG 
TCCTTGGAAT ATCAGCAGTT TGTAGCCAAT GTGGAAGAGG AGGAAGCCTG GATCAATGAG 
AAAATGACCC TGGTGGCCAG CGAAGATTAT GGCGACACTC TTGCTGCCAT CCAGGGCTTA 
CTGAAGAAAC ATGAAGCTTT TGAGACAGAC TTCACCGTCC ACAAGGATCG CGTGAATGAC 
GTCTGCACCA ATGGACAAGA CCTCATTAAG AAGAACAATC ACCATGAGGA GAACATCTCT 
TCAAAGATGA AGGGCCTGAA CGGGAAAGTG TCAGACCTGG AGAAAGCTGC AGCCCAGAGA 
AAGGCGAAGC TGGATGAGAA CTCGGCCTTC CTTCAGTTCA ACTGGAAGGC GGACGTGGTG 
GAGTCCTGGA TCGGTGAAAA GGAGAACAGC TTGAAGACAG ATGATTATGG CCGAGACCTG 
TCTTCTGTGC AGACGCTCCT CACCAAACAG GAAACTTTTG ACGCTGGGCT GCAGGCCTTC 
CAGCAGGAAG GCATTGCCAA CATCACTGCC CTCAAAGATC AGCTTCTCGC CGCCAAACAC 
GTTCAGTCCA AGGCCATCGA GGCCCGGCAC GCCTCCCTCA TGAAGAGGTG GAGCCAGCTT 
CTGGCCAACT CAGCCGCCCG CAAGAAGAAG CTTCTGGAGG CGCAGAGTCA CTTCCGCAAG 
GTGGAGGACC TCTTCCTGAC CTTTGCCAAA AAGGCTTCTG CCTTCAACAG CTGGTTTGAA 
AATGCAGAGG AGGACTTAAC AGACCCCGTG CGCTGCAACT CCTTGGAAGA AATCAAAGCT 
TTGCGCGAGG CCCACGACGC CTTCCGCTCC TCCCTCAGCT CTGCCCAGGC TGACTTCAAC 
CAGCTGGCCG AGCTGGACCG CCAGATCAAG AGCTTCCGCG TAGCCTCCAA CCCCTACACC 
TGGTTTACCA TGGAGGCCCT GGAGGAGACC TGGAGGAACC TACAGAAAAT CATCAAGGAG 
AGGGAGCTGG AGCTGCAGAA GGAACAGCGG CGGCAGGAGG AGAACGACAA GCTGCGCCAG 
GAGTTTGCCC AGCACGCCAA CGCCTTCCAC CAGTGGATCC AAGAGACCAG GACATACCTC 
CTCGATGGGT CCTGTATGGT GGAAGAGTCG GGGACCCTCG AATCCCAGCT TGAAGCTACC 
AAACGCAAGC ACCAGGAAAT CCGAGCCATG AGAAGTCAGC TCAAAAAGAT CGAGGACCTG 
GGGGCCGCCA TGGAGGAGGC CCTCATCCTG GACAACAAGT ACACGGAGCA CAGCACCGTG 
GGCCTCGCCC AGCAGTGGGA CCAGCTGGAC CAGCTGGGCA TGCGCATGCA GCACAACCTG 
GAGCAGCAGA TCCAGGCCAG GAACACAACA GGTGTGACTG AGGAGGCCCT CAAAGAATTC 
AGCATGATGT TTAAACACTT TGACAAGGAC AAGTCTGGCA GGCTGAACCA TCAGGAGTTC 
AAATCTTGCC TGCGCTCCCT GGGCTATGAC CTGCCCATGG TGGAGGAAGG GGAACCTGAC 
CCTGAGTTCG AGGCAATCCT GGACACGGTG GATCCGAACA GAGATGGCCA TGTCTCCCTG 
CAAGAATACA TGGCTTTCAT GATCAGCCGC GAAACTGAGA ACGTCAAGTC CAGCGAGGAG 
ATTGAGAGCG CCTTCCGGGC CCTCAGCTCA GAGGGAAAGC CTTACGTGAC CAAGGAGGAG 
CTCTACCAGA ACCTGACCCG GGAACAAGCC GACTACTGCG TCTCCCACAT GAAGCCCTAC 
GTGGACGGCA AGGGCCGCGA GCTCCCCACC GCGTTCGACT ACGTGGAGTT CACCCGCTCG 
CTTTTCGTGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_009457423.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009455698.2 Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X6, mRNA.

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CloneID	OPc89634
Clone ID Related Accession (Same CDS sequence)	XM_009457423.2
Accession Version	XM_009457423.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7452bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006476.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009457423.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAATTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAATG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA ACACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCCC TGCTAGAGGC AGATGTGGCT 
GCTCATCAGG ACCGAATTGA TGGCATTACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATGTTGAGGA TGAGGAGACG TGGATTCGAG AGAAGGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCAGCT GCTTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AGACACGCAT AACTAAGGAG GCCGGCAGTG TATCTCTGCG TATGAAGCAG 
GTGGAAGAAC TATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAGGC TCTAAACACA GACAATTATG GACATGATCT TGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAAT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCGTTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAAATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACGGCAAG TGATGAGTCA TACAAGGATC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCACCA GAAGCACCAG GCTTTTGAAG CAGAGCTGCA TGCCAACGCT 
GACCGGATCC GTGGGGTTAT CGACATGGGC AACTCCCTCA TTGAACGTGG AGCCTGTGCC 
GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCTTAG CTGACCAGTG GCAATTCTTG 
GTGCAAAAGT CAGCGGAAAA GAGCCAGAAA CTGAAAGAAG CCAACAAGCA GCAGAACTTC 
AACACAGGGA TCAAGGACTT TGACTTCTGG CTGTCTGAGG TGGAGGCCCT GCTGGCATCC 
GAAGATTATG GCAAAGACCT GGCTTCTGTG AACAACCTGC TGAAAAAGCA TCAACTGCTG 
GAAGCAGATA TATCTGCCCA TGAGGATCGC CTGAAGGACC TGAACAGCCA GGCAGACAGC 
CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTAAAGG ACAAGAGGGA CACCATCAAC 
GGGCGCTTCC AGAAGATCAA GAGCATGGCG GCCTCCCGGC GAGCCAAGCT GAATGAATCC 
CATCGCCTGC ACCAGTTCTT CCGGGACATG GATGACGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGGGCTCGGA GGACTACGGC CGGGACCTAA CCGGCGTGCA GAACCTGAGG 
AAGAAGCACA AGCGCCTGGA AGCAGAACTG GCTGCGCATG AGCCGGCTAT TCAGGGTGTC 
CTGGACACTG GCAAGAAGCT GTCCGATGAT AACACCATCG GGAAAGAGGA GATCCAGCAG 
CGGCTGGCGC AGTTTGTGGA GCACTGGAAA GAGCTGAAGC AGCTGGCAGC TGCCCGGGGT 
CAGCGGCTGG AAGAGTCCTT GGAATATCAG CAGTTTGTAG CCAATGTGGA AGAGGAGGAA 
GCCTGGATCA ATGAGAAAAT GACCCTGGTG GCCAGCGAAG ATTATGGCGA CACTCTTGCT 
GCCATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC CGTCCACAAG 
GATCGCGTGA ATGACGTCTG CACCAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCAAA GATGAAGGGC CTGAACGGGA AAGTGTCAGA CCTGGAGAAA 
GCTGCAGCCC AGAGAAAGGC GAAGCTGGAT GAGAACTCGG CCTTCCTTCA GTTCAACTGG 
AAGGCGGACG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGCCGAG ACCTGTCTTC TGTGCAGACG CTCCTCACCA AACAGGAAAC TTTTGACGCT 
GGGCTGCAGG CCTTCCAGCA GGAAGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTT 
CTCGCCGCCA AACACGTTCA GTCCAAGGCC ATCGAGGCCC GGCACGCCTC CCTCATGAAG 
AGGTGGAGCC AGCTTCTGGC CAACTCAGCC GCCCGCAAGA AGAAGCTTCT GGAGGCGCAG 
AGTCACTTCC GCAAGGTGGA GGACCTCTTC CTGACCTTTG CCAAAAAGGC TTCTGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CCGTGCGCTG CAACTCCTTG 
GAAGAAATCA AAGCTTTGCG CGAGGCCCAC GACGCCTTCC GCTCCTCCCT CAGCTCTGCC 
CAGGCTGACT TCAACCAGCT GGCCGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTAGCC 
TCCAACCCCT ACACCTGGTT TACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTACAG 
AAAATCATCA AGGAGAGGGA GCTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT TGCCCAGCAC GCCAACGCCT TCCACCAGTG GATCCAAGAG 
ACCAGGTCCT GTATGGTGGA AGAGTCGGGG ACCCTCGAAT CCCAGCTTGA AGCTACCAAA 
CGCAAGCACC AGGAAATCCG AGCCATGAGA AGTCAGCTCA AAAAGATCGA GGACCTGGGG 
GCCGCCATGG AGGAGGCCCT CATCCTGGAC AACAAGTACA CGGAGCACAG CACCGTGGGC 
CTCGCCCAGC AGTGGGACCA GCTGGACCAG CTGGGCATGC GCATGCAGCA CAACCTGGAG 
CAGCAGATCC AGGCCAGGAA CACAACAGGT GTGACTGAGG AGGCCCTCAA AGAATTCAGC 
ATGATGTTTA AACACTTTGA CAAGGACAAG TCTGGCAGGC TGAACCATCA GGAGTTCAAA 
TCTTGCCTGC GCTCCCTGGG CTATGACCTG CCCATGGTGG AGGAAGGGGA ACCTGACCCT 
GAGTTCGAGG CAATCCTGGA CACGGTGGAT CCGAACAGAG ATGGCCATGT CTCCCTGCAA 
GAATACATGG CTTTCATGAT CAGCCGCGAA ACTGAGAACG TCAAGTCCAG CGAGGAGATT 
GAGAGCGCCT TCCGGGCCCT CAGCTCAGAG GGAAAGCCTT ACGTGACCAA GGAGGAGCTC 
TACCAGAACC TGACCCGGGA ACAAGCCGAC TACTGCGTCT CCCACATGAA GCCCTACGTG 
GACGGCAAGG GCCGCGAGCT CCCCACCGCG TTCGACTACG TGGAGTTCAC CCGCTCGCTT 
TTCGTGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009455698.2
CDS	121..7572
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRITKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_009457423.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009457423.2

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAATTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAATG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA ACACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCCC TGCTAGAGGC AGATGTGGCT 
GCTCATCAGG ACCGAATTGA TGGCATTACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATGTTGAGGA TGAGGAGACG TGGATTCGAG AGAAGGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCAGCT GCTTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AGACACGCAT AACTAAGGAG GCCGGCAGTG TATCTCTGCG TATGAAGCAG 
GTGGAAGAAC TATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAGGC TCTAAACACA GACAATTATG GACATGATCT TGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAAT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCGTTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAAATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACGGCAAG TGATGAGTCA TACAAGGATC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCACCA GAAGCACCAG GCTTTTGAAG CAGAGCTGCA TGCCAACGCT 
GACCGGATCC GTGGGGTTAT CGACATGGGC AACTCCCTCA TTGAACGTGG AGCCTGTGCC 
GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCTTAG CTGACCAGTG GCAATTCTTG 
GTGCAAAAGT CAGCGGAAAA GAGCCAGAAA CTGAAAGAAG CCAACAAGCA GCAGAACTTC 
AACACAGGGA TCAAGGACTT TGACTTCTGG CTGTCTGAGG TGGAGGCCCT GCTGGCATCC 
GAAGATTATG GCAAAGACCT GGCTTCTGTG AACAACCTGC TGAAAAAGCA TCAACTGCTG 
GAAGCAGATA TATCTGCCCA TGAGGATCGC CTGAAGGACC TGAACAGCCA GGCAGACAGC 
CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTAAAGG ACAAGAGGGA CACCATCAAC 
GGGCGCTTCC AGAAGATCAA GAGCATGGCG GCCTCCCGGC GAGCCAAGCT GAATGAATCC 
CATCGCCTGC ACCAGTTCTT CCGGGACATG GATGACGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGGGCTCGGA GGACTACGGC CGGGACCTAA CCGGCGTGCA GAACCTGAGG 
AAGAAGCACA AGCGCCTGGA AGCAGAACTG GCTGCGCATG AGCCGGCTAT TCAGGGTGTC 
CTGGACACTG GCAAGAAGCT GTCCGATGAT AACACCATCG GGAAAGAGGA GATCCAGCAG 
CGGCTGGCGC AGTTTGTGGA GCACTGGAAA GAGCTGAAGC AGCTGGCAGC TGCCCGGGGT 
CAGCGGCTGG AAGAGTCCTT GGAATATCAG CAGTTTGTAG CCAATGTGGA AGAGGAGGAA 
GCCTGGATCA ATGAGAAAAT GACCCTGGTG GCCAGCGAAG ATTATGGCGA CACTCTTGCT 
GCCATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC CGTCCACAAG 
GATCGCGTGA ATGACGTCTG CACCAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCAAA GATGAAGGGC CTGAACGGGA AAGTGTCAGA CCTGGAGAAA 
GCTGCAGCCC AGAGAAAGGC GAAGCTGGAT GAGAACTCGG CCTTCCTTCA GTTCAACTGG 
AAGGCGGACG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGCCGAG ACCTGTCTTC TGTGCAGACG CTCCTCACCA AACAGGAAAC TTTTGACGCT 
GGGCTGCAGG CCTTCCAGCA GGAAGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTT 
CTCGCCGCCA AACACGTTCA GTCCAAGGCC ATCGAGGCCC GGCACGCCTC CCTCATGAAG 
AGGTGGAGCC AGCTTCTGGC CAACTCAGCC GCCCGCAAGA AGAAGCTTCT GGAGGCGCAG 
AGTCACTTCC GCAAGGTGGA GGACCTCTTC CTGACCTTTG CCAAAAAGGC TTCTGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CCGTGCGCTG CAACTCCTTG 
GAAGAAATCA AAGCTTTGCG CGAGGCCCAC GACGCCTTCC GCTCCTCCCT CAGCTCTGCC 
CAGGCTGACT TCAACCAGCT GGCCGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTAGCC 
TCCAACCCCT ACACCTGGTT TACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTACAG 
AAAATCATCA AGGAGAGGGA GCTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT TGCCCAGCAC GCCAACGCCT TCCACCAGTG GATCCAAGAG 
ACCAGGTCCT GTATGGTGGA AGAGTCGGGG ACCCTCGAAT CCCAGCTTGA AGCTACCAAA 
CGCAAGCACC AGGAAATCCG AGCCATGAGA AGTCAGCTCA AAAAGATCGA GGACCTGGGG 
GCCGCCATGG AGGAGGCCCT CATCCTGGAC AACAAGTACA CGGAGCACAG CACCGTGGGC 
CTCGCCCAGC AGTGGGACCA GCTGGACCAG CTGGGCATGC GCATGCAGCA CAACCTGGAG 
CAGCAGATCC AGGCCAGGAA CACAACAGGT GTGACTGAGG AGGCCCTCAA AGAATTCAGC 
ATGATGTTTA AACACTTTGA CAAGGACAAG TCTGGCAGGC TGAACCATCA GGAGTTCAAA 
TCTTGCCTGC GCTCCCTGGG CTATGACCTG CCCATGGTGG AGGAAGGGGA ACCTGACCCT 
GAGTTCGAGG CAATCCTGGA CACGGTGGAT CCGAACAGAG ATGGCCATGT CTCCCTGCAA 
GAATACATGG CTTTCATGAT CAGCCGCGAA ACTGAGAACG TCAAGTCCAG CGAGGAGATT 
GAGAGCGCCT TCCGGGCCCT CAGCTCAGAG GGAAAGCCTT ACGTGACCAA GGAGGAGCTC 
TACCAGAACC TGACCCGGGA ACAAGCCGAC TACTGCGTCT CCCACATGAA GCCCTACGTG 
GACGGCAAGG GCCGCGAGCT CCCCACCGCG TTCGACTACG TGGAGTTCAC CCGCTCGCTT 
TTCGTGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_009457424.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009455699.2 Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X7, mRNA.

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CloneID	OPc89635
Clone ID Related Accession (Same CDS sequence)	XM_009457424.2
Accession Version	XM_009457424.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7437bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006476.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009457424.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAA TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AATGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAACACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCCCTGCTAG AGGCAGATGT GGCTGCTCAT CAGGACCGAA TTGATGGCAT TACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAGG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC AGCTGCTTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AGGCTCTAAA CACAGACAAT 
TATGGACATG ATCTTGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAATCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCGTT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAAATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACGG CAAGTGATGA GTCATACAAG 
GATCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAGGCTTTT 
GAAGCAGAGC TGCATGCCAA CGCTGACCGG ATCCGTGGGG TTATCGACAT GGGCAACTCC 
CTCATTGAAC GTGGAGCCTG TGCCGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
TTAGCTGACC AGTGGCAATT CTTGGTGCAA AAGTCAGCGG AAAAGAGCCA GAAACTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACA GGGATCAAGG ACTTTGACTT CTGGCTGTCT 
GAGGTGGAGG CCCTGCTGGC ATCCGAAGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC 
CTGCTGAAAA AGCATCAACT GCTGGAAGCA GATATATCTG CCCATGAGGA TCGCCTGAAG 
GACCTGAACA GCCAGGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTA 
AAGGACAAGA GGGACACCAT CAACGGGCGC TTCCAGAAGA TCAAGAGCAT GGCGGCCTCC 
CGGCGAGCCA AGCTGAATGA ATCCCATCGC CTGCACCAGT TCTTCCGGGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGGGCT CGGAGGACTA CGGCCGGGAC 
CTAACCGGCG TGCAGAACCT GAGGAAGAAG CACAAGCGCC TGGAAGCAGA ACTGGCTGCG 
CATGAGCCGG CTATTCAGGG TGTCCTGGAC ACTGGCAAGA AGCTGTCCGA TGATAACACC 
ATCGGGAAAG AGGAGATCCA GCAGCGGCTG GCGCAGTTTG TGGAGCACTG GAAAGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGTCAGCGG CTGGAAGAGT CCTTGGAATA TCAGCAGTTT 
GTAGCCAATG TGGAAGAGGA GGAAGCCTGG ATCAATGAGA AAATGACCCT GGTGGCCAGC 
GAAGATTATG GCGACACTCT TGCTGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACCGTCCA CAAGGATCGC GTGAATGACG TCTGCACCAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGCCTGAAC 
GGGAAAGTGT CAGACCTGGA GAAAGCTGCA GCCCAGAGAA AGGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA CTGGAAGGCG GACGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGC CGAGACCTGT CTTCTGTGCA GACGCTCCTC 
ACCAAACAGG AAACTTTTGA CGCTGGGCTG CAGGCCTTCC AGCAGGAAGG CATTGCCAAC 
ATCACTGCCC TCAAAGATCA GCTTCTCGCC GCCAAACACG TTCAGTCCAA GGCCATCGAG 
GCCCGGCACG CCTCCCTCAT GAAGAGGTGG AGCCAGCTTC TGGCCAACTC AGCCGCCCGC 
AAGAAGAAGC TTCTGGAGGC GCAGAGTCAC TTCCGCAAGG TGGAGGACCT CTTCCTGACC 
TTTGCCAAAA AGGCTTCTGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCCGTGC GCTGCAACTC CTTGGAAGAA ATCAAAGCTT TGCGCGAGGC CCACGACGCC 
TTCCGCTCCT CCCTCAGCTC TGCCCAGGCT GACTTCAACC AGCTGGCCGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT AGCCTCCAAC CCCTACACCT GGTTTACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT ACAGAAAATC ATCAAGGAGA GGGAGCTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTTGCCCA GCACGCCAAC 
GCCTTCCACC AGTGGATCCA AGAGACCAGG ACATACCTCC TCGATGGCAT AGCATATCGT 
CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGTATG 
GTGGAAGAGT CGGGGACCCT CGAATCCCAG CTTGAAGCTA CCAAACGCAA GCACCAGGAA 
ATCCGAGCCA TGAGAAGTCA GCTCAAAAAG ATCGAGGACC TGGGGGCCGC CATGGAGGAG 
GCCCTCATCC TGGACAACAA GTACACGGAG CACAGCACCG TGGGCCTCGC CCAGCAGTGG 
GACCAGCTGG ACCAGCTGGG CATGCGCATG CAGCACAACC TGGAGCAGCA GATCCAGGCC 
AGGAACACAA CAGGTGTGAC TGAGGAGGCC CTCAAAGAAT TCAGCATGAT GTTTAAACAC 
TTTGACAAGG ACAAGTCTGG CAGGCTGAAC CATCAGGAGT TCAAATCTTG CCTGCGCTCC 
CTGGGCTATG ACCTGCCCAT GGTGGAGGAA GGGGAACCTG ACCCTGAGTT CGAGGCAATC 
CTGGACACGG TGGATCCGAA CAGAGATGGC CATGTCTCCC TGCAAGAATA CATGGCTTTC 
ATGATCAGCC GCGAAACTGA GAACGTCAAG TCCAGCGAGG AGATTGAGAG CGCCTTCCGG 
GCCCTCAGCT CAGAGGGAAA GCCTTACGTG ACCAAGGAGG AGCTCTACCA GAACCTGACC 
CGGGAACAAG CCGACTACTG CGTCTCCCAC ATGAAGCCCT ACGTGGACGG CAAGGGCCGC 
GAGCTCCCCA CCGCGTTCGA CTACGTGGAG TTCACCCGCT CGCTTTTCGT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009455699.2
CDS	138..7574
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_009457424.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009457424.2

ORF Insert Sequence:

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAA TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AATGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAACACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCCCTGCTAG AGGCAGATGT GGCTGCTCAT CAGGACCGAA TTGATGGCAT TACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAGG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC AGCTGCTTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AGGCTCTAAA CACAGACAAT 
TATGGACATG ATCTTGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAATCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCGTT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAAATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACGG CAAGTGATGA GTCATACAAG 
GATCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAGGCTTTT 
GAAGCAGAGC TGCATGCCAA CGCTGACCGG ATCCGTGGGG TTATCGACAT GGGCAACTCC 
CTCATTGAAC GTGGAGCCTG TGCCGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
TTAGCTGACC AGTGGCAATT CTTGGTGCAA AAGTCAGCGG AAAAGAGCCA GAAACTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACA GGGATCAAGG ACTTTGACTT CTGGCTGTCT 
GAGGTGGAGG CCCTGCTGGC ATCCGAAGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC 
CTGCTGAAAA AGCATCAACT GCTGGAAGCA GATATATCTG CCCATGAGGA TCGCCTGAAG 
GACCTGAACA GCCAGGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTA 
AAGGACAAGA GGGACACCAT CAACGGGCGC TTCCAGAAGA TCAAGAGCAT GGCGGCCTCC 
CGGCGAGCCA AGCTGAATGA ATCCCATCGC CTGCACCAGT TCTTCCGGGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGGGCT CGGAGGACTA CGGCCGGGAC 
CTAACCGGCG TGCAGAACCT GAGGAAGAAG CACAAGCGCC TGGAAGCAGA ACTGGCTGCG 
CATGAGCCGG CTATTCAGGG TGTCCTGGAC ACTGGCAAGA AGCTGTCCGA TGATAACACC 
ATCGGGAAAG AGGAGATCCA GCAGCGGCTG GCGCAGTTTG TGGAGCACTG GAAAGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGTCAGCGG CTGGAAGAGT CCTTGGAATA TCAGCAGTTT 
GTAGCCAATG TGGAAGAGGA GGAAGCCTGG ATCAATGAGA AAATGACCCT GGTGGCCAGC 
GAAGATTATG GCGACACTCT TGCTGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACCGTCCA CAAGGATCGC GTGAATGACG TCTGCACCAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGCCTGAAC 
GGGAAAGTGT CAGACCTGGA GAAAGCTGCA GCCCAGAGAA AGGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA CTGGAAGGCG GACGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGC CGAGACCTGT CTTCTGTGCA GACGCTCCTC 
ACCAAACAGG AAACTTTTGA CGCTGGGCTG CAGGCCTTCC AGCAGGAAGG CATTGCCAAC 
ATCACTGCCC TCAAAGATCA GCTTCTCGCC GCCAAACACG TTCAGTCCAA GGCCATCGAG 
GCCCGGCACG CCTCCCTCAT GAAGAGGTGG AGCCAGCTTC TGGCCAACTC AGCCGCCCGC 
AAGAAGAAGC TTCTGGAGGC GCAGAGTCAC TTCCGCAAGG TGGAGGACCT CTTCCTGACC 
TTTGCCAAAA AGGCTTCTGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCCGTGC GCTGCAACTC CTTGGAAGAA ATCAAAGCTT TGCGCGAGGC CCACGACGCC 
TTCCGCTCCT CCCTCAGCTC TGCCCAGGCT GACTTCAACC AGCTGGCCGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT AGCCTCCAAC CCCTACACCT GGTTTACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT ACAGAAAATC ATCAAGGAGA GGGAGCTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTTGCCCA GCACGCCAAC 
GCCTTCCACC AGTGGATCCA AGAGACCAGG ACATACCTCC TCGATGGCAT AGCATATCGT 
CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGTATG 
GTGGAAGAGT CGGGGACCCT CGAATCCCAG CTTGAAGCTA CCAAACGCAA GCACCAGGAA 
ATCCGAGCCA TGAGAAGTCA GCTCAAAAAG ATCGAGGACC TGGGGGCCGC CATGGAGGAG 
GCCCTCATCC TGGACAACAA GTACACGGAG CACAGCACCG TGGGCCTCGC CCAGCAGTGG 
GACCAGCTGG ACCAGCTGGG CATGCGCATG CAGCACAACC TGGAGCAGCA GATCCAGGCC 
AGGAACACAA CAGGTGTGAC TGAGGAGGCC CTCAAAGAAT TCAGCATGAT GTTTAAACAC 
TTTGACAAGG ACAAGTCTGG CAGGCTGAAC CATCAGGAGT TCAAATCTTG CCTGCGCTCC 
CTGGGCTATG ACCTGCCCAT GGTGGAGGAA GGGGAACCTG ACCCTGAGTT CGAGGCAATC 
CTGGACACGG TGGATCCGAA CAGAGATGGC CATGTCTCCC TGCAAGAATA CATGGCTTTC 
ATGATCAGCC GCGAAACTGA GAACGTCAAG TCCAGCGAGG AGATTGAGAG CGCCTTCCGG 
GCCCTCAGCT CAGAGGGAAA GCCTTACGTG ACCAAGGAGG AGCTCTACCA GAACCTGACC 
CGGGAACAAG CCGACTACTG CGTCTCCCAC ATGAAGCCCT ACGTGGACGG CAAGGGCCGC 
GAGCTCCCCA CCGCGTTCGA CTACGTGGAG TTCACCCGCT CGCTTTTCGT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_009457425.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009455700.2 Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X8, mRNA.

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CloneID	OPc89636
Clone ID Related Accession (Same CDS sequence)	XM_009457425.2
Accession Version	XM_009457425.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7434bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006476.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009457425.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAA TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AATGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAACACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCCCTGCTAG AGGCAGATGT GGCTGCTCAT CAGGACCGAA TTGATGGCAT TACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAGG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC AGCTGCTTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAGACAC GCATAACTAA GGAGGCCGGC 
AGTGTATCTC TGCGTATGAA GCAGGTGGAA GAACTATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AGGCTCTAAA CACAGACAAT 
TATGGACATG ATCTTGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAATCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCGTT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAAATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACGG CAAGTGATGA GTCATACAAG 
GATCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAGGCTTTT 
GAAGCAGAGC TGCATGCCAA CGCTGACCGG ATCCGTGGGG TTATCGACAT GGGCAACTCC 
CTCATTGAAC GTGGAGCCTG TGCCGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
TTAGCTGACC AGTGGCAATT CTTGGTGCAA AAGTCAGCGG AAAAGAGCCA GAAACTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACA GGGATCAAGG ACTTTGACTT CTGGCTGTCT 
GAGGTGGAGG CCCTGCTGGC ATCCGAAGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC 
CTGCTGAAAA AGCATCAACT GCTGGAAGCA GATATATCTG CCCATGAGGA TCGCCTGAAG 
GACCTGAACA GCCAGGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTA 
AAGGACAAGA GGGACACCAT CAACGGGCGC TTCCAGAAGA TCAAGAGCAT GGCGGCCTCC 
CGGCGAGCCA AGCTGAATGA ATCCCATCGC CTGCACCAGT TCTTCCGGGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGGGCT CGGAGGACTA CGGCCGGGAC 
CTAACCGGCG TGCAGAACCT GAGGAAGAAG CACAAGCGCC TGGAAGCAGA ACTGGCTGCG 
CATGAGCCGG CTATTCAGGG TGTCCTGGAC ACTGGCAAGA AGCTGTCCGA TGATAACACC 
ATCGGGAAAG AGGAGATCCA GCAGCGGCTG GCGCAGTTTG TGGAGCACTG GAAAGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGTCAGCGG CTGGAAGAGT CCTTGGAATA TCAGCAGTTT 
GTAGCCAATG TGGAAGAGGA GGAAGCCTGG ATCAATGAGA AAATGACCCT GGTGGCCAGC 
GAAGATTATG GCGACACTCT TGCTGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACCGTCCA CAAGGATCGC GTGAATGACG TCTGCACCAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGCCTGAAC 
GGGAAAGTGT CAGACCTGGA GAAAGCTGCA GCCCAGAGAA AGGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA CTGGAAGGCG GACGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGC CGAGACCTGT CTTCTGTGCA GACGCTCCTC 
ACCAAACAGG AAACTTTTGA CGCTGGGCTG CAGGCCTTCC AGCAGGAAGG CATTGCCAAC 
ATCACTGCCC TCAAAGATCA GCTTCTCGCC GCCAAACACG TTCAGTCCAA GGCCATCGAG 
GCCCGGCACG CCTCCCTCAT GAAGAGGTGG AGCCAGCTTC TGGCCAACTC AGCCGCCCGC 
AAGAAGAAGC TTCTGGAGGC GCAGAGTCAC TTCCGCAAGG TGGAGGACCT CTTCCTGACC 
TTTGCCAAAA AGGCTTCTGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCCGTGC GCTGCAACTC CTTGGAAGAA ATCAAAGCTT TGCGCGAGGC CCACGACGCC 
TTCCGCTCCT CCCTCAGCTC TGCCCAGGCT GACTTCAACC AGCTGGCCGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT AGCCTCCAAC CCCTACACCT GGTTTACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT ACAGAAAATC ATCAAGGAGA GGGAGCTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTTGCCCA GCACGCCAAC 
GCCTTCCACC AGTGGATCCA AGAGACCAGG ACATACCTCC TCGATGGGTC CTGTATGGTG 
GAAGAGTCGG GGACCCTCGA ATCCCAGCTT GAAGCTACCA AACGCAAGCA CCAGGAAATC 
CGAGCCATGA GAAGTCAGCT CAAAAAGATC GAGGACCTGG GGGCCGCCAT GGAGGAGGCC 
CTCATCCTGG ACAACAAGTA CACGGAGCAC AGCACCGTGG GCCTCGCCCA GCAGTGGGAC 
CAGCTGGACC AGCTGGGCAT GCGCATGCAG CACAACCTGG AGCAGCAGAT CCAGGCCAGG 
AACACAACAG GTGTGACTGA GGAGGCCCTC AAAGAATTCA GCATGATGTT TAAACACTTT 
GACAAGGACA AGTCTGGCAG GCTGAACCAT CAGGAGTTCA AATCTTGCCT GCGCTCCCTG 
GGCTATGACC TGCCCATGGT GGAGGAAGGG GAACCTGACC CTGAGTTCGA GGCAATCCTG 
GACACGGTGG ATCCGAACAG AGATGGCCAT GTCTCCCTGC AAGAATACAT GGCTTTCATG 
ATCAGCCGCG AAACTGAGAA CGTCAAGTCC AGCGAGGAGA TTGAGAGCGC CTTCCGGGCC 
CTCAGCTCAG AGGGAAAGCC TTACGTGACC AAGGAGGAGC TCTACCAGAA CCTGACCCGG 
GAACAAGCCG ACTACTGCGT CTCCCACATG AAGCCCTACG TGGACGGCAA GGGCCGCGAG 
CTCCCCACCG CGTTCGACTA CGTGGAGTTC ACCCGCTCGC TTTTCGTGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009455700.2
CDS	137..7570
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRITKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_009457425.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009457425.2

ORF Insert Sequence:

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAA TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AATGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAACACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCCCTGCTAG AGGCAGATGT GGCTGCTCAT CAGGACCGAA TTGATGGCAT TACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAGG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC AGCTGCTTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAGACAC GCATAACTAA GGAGGCCGGC 
AGTGTATCTC TGCGTATGAA GCAGGTGGAA GAACTATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AGGCTCTAAA CACAGACAAT 
TATGGACATG ATCTTGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAATCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCGTT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAAATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACGG CAAGTGATGA GTCATACAAG 
GATCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ACCAGAAGCA CCAGGCTTTT 
GAAGCAGAGC TGCATGCCAA CGCTGACCGG ATCCGTGGGG TTATCGACAT GGGCAACTCC 
CTCATTGAAC GTGGAGCCTG TGCCGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC 
TTAGCTGACC AGTGGCAATT CTTGGTGCAA AAGTCAGCGG AAAAGAGCCA GAAACTGAAA 
GAAGCCAACA AGCAGCAGAA CTTCAACACA GGGATCAAGG ACTTTGACTT CTGGCTGTCT 
GAGGTGGAGG CCCTGCTGGC ATCCGAAGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC 
CTGCTGAAAA AGCATCAACT GCTGGAAGCA GATATATCTG CCCATGAGGA TCGCCTGAAG 
GACCTGAACA GCCAGGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTA 
AAGGACAAGA GGGACACCAT CAACGGGCGC TTCCAGAAGA TCAAGAGCAT GGCGGCCTCC 
CGGCGAGCCA AGCTGAATGA ATCCCATCGC CTGCACCAGT TCTTCCGGGA CATGGATGAC 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGGGCT CGGAGGACTA CGGCCGGGAC 
CTAACCGGCG TGCAGAACCT GAGGAAGAAG CACAAGCGCC TGGAAGCAGA ACTGGCTGCG 
CATGAGCCGG CTATTCAGGG TGTCCTGGAC ACTGGCAAGA AGCTGTCCGA TGATAACACC 
ATCGGGAAAG AGGAGATCCA GCAGCGGCTG GCGCAGTTTG TGGAGCACTG GAAAGAGCTG 
AAGCAGCTGG CAGCTGCCCG GGGTCAGCGG CTGGAAGAGT CCTTGGAATA TCAGCAGTTT 
GTAGCCAATG TGGAAGAGGA GGAAGCCTGG ATCAATGAGA AAATGACCCT GGTGGCCAGC 
GAAGATTATG GCGACACTCT TGCTGCCATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT 
GAGACAGACT TCACCGTCCA CAAGGATCGC GTGAATGACG TCTGCACCAA TGGACAAGAC 
CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGCCTGAAC 
GGGAAAGTGT CAGACCTGGA GAAAGCTGCA GCCCAGAGAA AGGCGAAGCT GGATGAGAAC 
TCGGCCTTCC TTCAGTTCAA CTGGAAGGCG GACGTGGTGG AGTCCTGGAT CGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGC CGAGACCTGT CTTCTGTGCA GACGCTCCTC 
ACCAAACAGG AAACTTTTGA CGCTGGGCTG CAGGCCTTCC AGCAGGAAGG CATTGCCAAC 
ATCACTGCCC TCAAAGATCA GCTTCTCGCC GCCAAACACG TTCAGTCCAA GGCCATCGAG 
GCCCGGCACG CCTCCCTCAT GAAGAGGTGG AGCCAGCTTC TGGCCAACTC AGCCGCCCGC 
AAGAAGAAGC TTCTGGAGGC GCAGAGTCAC TTCCGCAAGG TGGAGGACCT CTTCCTGACC 
TTTGCCAAAA AGGCTTCTGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCCGTGC GCTGCAACTC CTTGGAAGAA ATCAAAGCTT TGCGCGAGGC CCACGACGCC 
TTCCGCTCCT CCCTCAGCTC TGCCCAGGCT GACTTCAACC AGCTGGCCGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT AGCCTCCAAC CCCTACACCT GGTTTACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT ACAGAAAATC ATCAAGGAGA GGGAGCTGGA GCTGCAGAAG 
GAACAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTTGCCCA GCACGCCAAC 
GCCTTCCACC AGTGGATCCA AGAGACCAGG ACATACCTCC TCGATGGGTC CTGTATGGTG 
GAAGAGTCGG GGACCCTCGA ATCCCAGCTT GAAGCTACCA AACGCAAGCA CCAGGAAATC 
CGAGCCATGA GAAGTCAGCT CAAAAAGATC GAGGACCTGG GGGCCGCCAT GGAGGAGGCC 
CTCATCCTGG ACAACAAGTA CACGGAGCAC AGCACCGTGG GCCTCGCCCA GCAGTGGGAC 
CAGCTGGACC AGCTGGGCAT GCGCATGCAG CACAACCTGG AGCAGCAGAT CCAGGCCAGG 
AACACAACAG GTGTGACTGA GGAGGCCCTC AAAGAATTCA GCATGATGTT TAAACACTTT 
GACAAGGACA AGTCTGGCAG GCTGAACCAT CAGGAGTTCA AATCTTGCCT GCGCTCCCTG 
GGCTATGACC TGCCCATGGT GGAGGAAGGG GAACCTGACC CTGAGTTCGA GGCAATCCTG 
GACACGGTGG ATCCGAACAG AGATGGCCAT GTCTCCCTGC AAGAATACAT GGCTTTCATG 
ATCAGCCGCG AAACTGAGAA CGTCAAGTCC AGCGAGGAGA TTGAGAGCGC CTTCCGGGCC 
CTCAGCTCAG AGGGAAAGCC TTACGTGACC AAGGAGGAGC TCTACCAGAA CCTGACCCGG 
GAACAAGCCG ACTACTGCGT CTCCCACATG AAGCCCTACG TGGACGGCAA GGGCCGCGAG 
CTCCCCACCG CGTTCGACTA CGTGGAGTTC ACCCGCTCGC TTTTCGTGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_009457427.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009455702.2 Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X9, mRNA.

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CloneID	OPc89637
Clone ID Related Accession (Same CDS sequence)	XM_009457427.2
Accession Version	XM_009457427.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7410bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006476.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009457427.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAATTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAATG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA ACACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCCC TGCTAGAGGC AGATGTGGCT 
GCTCATCAGG ACCGAATTGA TGGCATTACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATGTTGAGGA TGAGGAGACG TGGATTCGAG AGAAGGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCAGCT GCTTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAGGC TCTAAACACA GACAATTATG GACATGATCT TGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAAT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCGTTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAAATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACGGCAAG TGATGAGTCA TACAAGGATC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCACCA GAAGCACCAG GCTTTTGAAG CAGAGCTGCA TGCCAACGCT 
GACCGGATCC GTGGGGTTAT CGACATGGGC AACTCCCTCA TTGAACGTGG AGCCTGTGCC 
GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCTTAG CTGACCAGTG GCAATTCTTG 
GTGCAAAAGT CAGCGGAAAA GAGCCAGAAA CTGAAAGAAG CCAACAAGCA GCAGAACTTC 
AACACAGGGA TCAAGGACTT TGACTTCTGG CTGTCTGAGG TGGAGGCCCT GCTGGCATCC 
GAAGATTATG GCAAAGACCT GGCTTCTGTG AACAACCTGC TGAAAAAGCA TCAACTGCTG 
GAAGCAGATA TATCTGCCCA TGAGGATCGC CTGAAGGACC TGAACAGCCA GGCAGACAGC 
CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTAAAGG ACAAGAGGGA CACCATCAAC 
GGGCGCTTCC AGAAGATCAA GAGCATGGCG GCCTCCCGGC GAGCCAAGCT GAATGAATCC 
CATCGCCTGC ACCAGTTCTT CCGGGACATG GATGACGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGGGCTCGGA GGACTACGGC CGGGACCTAA CCGGCGTGCA GAACCTGAGG 
AAGAAGCACA AGCGCCTGGA AGCAGAACTG GCTGCGCATG AGCCGGCTAT TCAGGGTGTC 
CTGGACACTG GCAAGAAGCT GTCCGATGAT AACACCATCG GGAAAGAGGA GATCCAGCAG 
CGGCTGGCGC AGTTTGTGGA GCACTGGAAA GAGCTGAAGC AGCTGGCAGC TGCCCGGGGT 
CAGCGGCTGG AAGAGTCCTT GGAATATCAG CAGTTTGTAG CCAATGTGGA AGAGGAGGAA 
GCCTGGATCA ATGAGAAAAT GACCCTGGTG GCCAGCGAAG ATTATGGCGA CACTCTTGCT 
GCCATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC CGTCCACAAG 
GATCGCGTGA ATGACGTCTG CACCAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCAAA GATGAAGGGC CTGAACGGGA AAGTGTCAGA CCTGGAGAAA 
GCTGCAGCCC AGAGAAAGGC GAAGCTGGAT GAGAACTCGG CCTTCCTTCA GTTCAACTGG 
AAGGCGGACG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGCCGAG ACCTGTCTTC TGTGCAGACG CTCCTCACCA AACAGGAAAC TTTTGACGCT 
GGGCTGCAGG CCTTCCAGCA GGAAGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTT 
CTCGCCGCCA AACACGTTCA GTCCAAGGCC ATCGAGGCCC GGCACGCCTC CCTCATGAAG 
AGGTGGAGCC AGCTTCTGGC CAACTCAGCC GCCCGCAAGA AGAAGCTTCT GGAGGCGCAG 
AGTCACTTCC GCAAGGTGGA GGACCTCTTC CTGACCTTTG CCAAAAAGGC TTCTGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CCGTGCGCTG CAACTCCTTG 
GAAGAAATCA AAGCTTTGCG CGAGGCCCAC GACGCCTTCC GCTCCTCCCT CAGCTCTGCC 
CAGGCTGACT TCAACCAGCT GGCCGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTAGCC 
TCCAACCCCT ACACCTGGTT TACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTACAG 
AAAATCATCA AGGAGAGGGA GCTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT TGCCCAGCAC GCCAACGCCT TCCACCAGTG GATCCAAGAG 
ACCAGGACAT ACCTCCTCGA TGGGTCCTGT ATGGTGGAAG AGTCGGGGAC CCTCGAATCC 
CAGCTTGAAG CTACCAAACG CAAGCACCAG GAAATCCGAG CCATGAGAAG TCAGCTCAAA 
AAGATCGAGG ACCTGGGGGC CGCCATGGAG GAGGCCCTCA TCCTGGACAA CAAGTACACG 
GAGCACAGCA CCGTGGGCCT CGCCCAGCAG TGGGACCAGC TGGACCAGCT GGGCATGCGC 
ATGCAGCACA ACCTGGAGCA GCAGATCCAG GCCAGGAACA CAACAGGTGT GACTGAGGAG 
GCCCTCAAAG AATTCAGCAT GATGTTTAAA CACTTTGACA AGGACAAGTC TGGCAGGCTG 
AACCATCAGG AGTTCAAATC TTGCCTGCGC TCCCTGGGCT ATGACCTGCC CATGGTGGAG 
GAAGGGGAAC CTGACCCTGA GTTCGAGGCA ATCCTGGACA CGGTGGATCC GAACAGAGAT 
GGCCATGTCT CCCTGCAAGA ATACATGGCT TTCATGATCA GCCGCGAAAC TGAGAACGTC 
AAGTCCAGCG AGGAGATTGA GAGCGCCTTC CGGGCCCTCA GCTCAGAGGG AAAGCCTTAC 
GTGACCAAGG AGGAGCTCTA CCAGAACCTG ACCCGGGAAC AAGCCGACTA CTGCGTCTCC 
CACATGAAGC CCTACGTGGA CGGCAAGGGC CGCGAGCTCC CCACCGCGTT CGACTACGTG 
GAGTTCACCC GCTCGCTTTT CGTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009455702.2
CDS	121..7530
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_009457427.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009457427.2

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAATTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAATG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA ACACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCCC TGCTAGAGGC AGATGTGGCT 
GCTCATCAGG ACCGAATTGA TGGCATTACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATGTTGAGGA TGAGGAGACG TGGATTCGAG AGAAGGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCAGCT GCTTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAGGC TCTAAACACA GACAATTATG GACATGATCT TGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAAT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCGTTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAAATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACGGCAAG TGATGAGTCA TACAAGGATC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCACCA GAAGCACCAG GCTTTTGAAG CAGAGCTGCA TGCCAACGCT 
GACCGGATCC GTGGGGTTAT CGACATGGGC AACTCCCTCA TTGAACGTGG AGCCTGTGCC 
GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCTTAG CTGACCAGTG GCAATTCTTG 
GTGCAAAAGT CAGCGGAAAA GAGCCAGAAA CTGAAAGAAG CCAACAAGCA GCAGAACTTC 
AACACAGGGA TCAAGGACTT TGACTTCTGG CTGTCTGAGG TGGAGGCCCT GCTGGCATCC 
GAAGATTATG GCAAAGACCT GGCTTCTGTG AACAACCTGC TGAAAAAGCA TCAACTGCTG 
GAAGCAGATA TATCTGCCCA TGAGGATCGC CTGAAGGACC TGAACAGCCA GGCAGACAGC 
CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTAAAGG ACAAGAGGGA CACCATCAAC 
GGGCGCTTCC AGAAGATCAA GAGCATGGCG GCCTCCCGGC GAGCCAAGCT GAATGAATCC 
CATCGCCTGC ACCAGTTCTT CCGGGACATG GATGACGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGGGCTCGGA GGACTACGGC CGGGACCTAA CCGGCGTGCA GAACCTGAGG 
AAGAAGCACA AGCGCCTGGA AGCAGAACTG GCTGCGCATG AGCCGGCTAT TCAGGGTGTC 
CTGGACACTG GCAAGAAGCT GTCCGATGAT AACACCATCG GGAAAGAGGA GATCCAGCAG 
CGGCTGGCGC AGTTTGTGGA GCACTGGAAA GAGCTGAAGC AGCTGGCAGC TGCCCGGGGT 
CAGCGGCTGG AAGAGTCCTT GGAATATCAG CAGTTTGTAG CCAATGTGGA AGAGGAGGAA 
GCCTGGATCA ATGAGAAAAT GACCCTGGTG GCCAGCGAAG ATTATGGCGA CACTCTTGCT 
GCCATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC CGTCCACAAG 
GATCGCGTGA ATGACGTCTG CACCAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCAAA GATGAAGGGC CTGAACGGGA AAGTGTCAGA CCTGGAGAAA 
GCTGCAGCCC AGAGAAAGGC GAAGCTGGAT GAGAACTCGG CCTTCCTTCA GTTCAACTGG 
AAGGCGGACG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGCCGAG ACCTGTCTTC TGTGCAGACG CTCCTCACCA AACAGGAAAC TTTTGACGCT 
GGGCTGCAGG CCTTCCAGCA GGAAGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTT 
CTCGCCGCCA AACACGTTCA GTCCAAGGCC ATCGAGGCCC GGCACGCCTC CCTCATGAAG 
AGGTGGAGCC AGCTTCTGGC CAACTCAGCC GCCCGCAAGA AGAAGCTTCT GGAGGCGCAG 
AGTCACTTCC GCAAGGTGGA GGACCTCTTC CTGACCTTTG CCAAAAAGGC TTCTGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CCGTGCGCTG CAACTCCTTG 
GAAGAAATCA AAGCTTTGCG CGAGGCCCAC GACGCCTTCC GCTCCTCCCT CAGCTCTGCC 
CAGGCTGACT TCAACCAGCT GGCCGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTAGCC 
TCCAACCCCT ACACCTGGTT TACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTACAG 
AAAATCATCA AGGAGAGGGA GCTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT TGCCCAGCAC GCCAACGCCT TCCACCAGTG GATCCAAGAG 
ACCAGGACAT ACCTCCTCGA TGGGTCCTGT ATGGTGGAAG AGTCGGGGAC CCTCGAATCC 
CAGCTTGAAG CTACCAAACG CAAGCACCAG GAAATCCGAG CCATGAGAAG TCAGCTCAAA 
AAGATCGAGG ACCTGGGGGC CGCCATGGAG GAGGCCCTCA TCCTGGACAA CAAGTACACG 
GAGCACAGCA CCGTGGGCCT CGCCCAGCAG TGGGACCAGC TGGACCAGCT GGGCATGCGC 
ATGCAGCACA ACCTGGAGCA GCAGATCCAG GCCAGGAACA CAACAGGTGT GACTGAGGAG 
GCCCTCAAAG AATTCAGCAT GATGTTTAAA CACTTTGACA AGGACAAGTC TGGCAGGCTG 
AACCATCAGG AGTTCAAATC TTGCCTGCGC TCCCTGGGCT ATGACCTGCC CATGGTGGAG 
GAAGGGGAAC CTGACCCTGA GTTCGAGGCA ATCCTGGACA CGGTGGATCC GAACAGAGAT 
GGCCATGTCT CCCTGCAAGA ATACATGGCT TTCATGATCA GCCGCGAAAC TGAGAACGTC 
AAGTCCAGCG AGGAGATTGA GAGCGCCTTC CGGGCCCTCA GCTCAGAGGG AAAGCCTTAC 
GTGACCAAGG AGGAGCTCTA CCAGAACCTG ACCCGGGAAC AAGCCGACTA CTGCGTCTCC 
CACATGAAGC CCTACGTGGA CGGCAAGGGC CGCGAGCTCC CCACCGCGTT CGACTACGTG 
GAGTTCACCC GCTCGCTTTT CGTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_016961798.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016817287.1 Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X10, mRNA.

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CloneID	OPc89638
Clone ID Related Accession (Same CDS sequence)	XM_016961798.1
Accession Version	XM_016961798.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7395bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006476.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAATTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAATG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA ACACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCCC TGCTAGAGGC AGATGTGGCT 
GCTCATCAGG ACCGAATTGA TGGCATTACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATGTTGAGGA TGAGGAGACG TGGATTCGAG AGAAGGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCAGCT GCTTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAGGC TCTAAACACA GACAATTATG GACATGATCT TGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAAT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCGTTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAAATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACGGCAAG TGATGAGTCA TACAAGGATC CCACCAACAT CCAGAGCAAG 
CACCAGAAGC ACCAGGCTTT TGAAGCAGAG CTGCATGCCA ACGCTGACCG GATCCGTGGG 
GTTATCGACA TGGGCAACTC CCTCATTGAA CGTGGAGCCT GTGCCGGCAG TGAGGATGCT 
GTCAAGGCCC GCCTGGCTGC CTTAGCTGAC CAGTGGCAAT TCTTGGTGCA AAAGTCAGCG 
GAAAAGAGCC AGAAACTGAA AGAAGCCAAC AAGCAGCAGA ACTTCAACAC AGGGATCAAG 
GACTTTGACT TCTGGCTGTC TGAGGTGGAG GCCCTGCTGG CATCCGAAGA TTATGGCAAA 
GACCTGGCTT CTGTGAACAA CCTGCTGAAA AAGCATCAAC TGCTGGAAGC AGATATATCT 
GCCCATGAGG ATCGCCTGAA GGACCTGAAC AGCCAGGCAG ACAGCCTGAT GACCAGCAGT 
GCCTTCGACA CCTCCCAAGT AAAGGACAAG AGGGACACCA TCAACGGGCG CTTCCAGAAG 
ATCAAGAGCA TGGCGGCCTC CCGGCGAGCC AAGCTGAATG AATCCCATCG CCTGCACCAG 
TTCTTCCGGG ACATGGATGA CGAGGAGTCC TGGATCAAGG AGAAGAAGCT GCTGGTGGGC 
TCGGAGGACT ACGGCCGGGA CCTAACCGGC GTGCAGAACC TGAGGAAGAA GCACAAGCGC 
CTGGAAGCAG AACTGGCTGC GCATGAGCCG GCTATTCAGG GTGTCCTGGA CACTGGCAAG 
AAGCTGTCCG ATGATAACAC CATCGGGAAA GAGGAGATCC AGCAGCGGCT GGCGCAGTTT 
GTGGAGCACT GGAAAGAGCT GAAGCAGCTG GCAGCTGCCC GGGGTCAGCG GCTGGAAGAG 
TCCTTGGAAT ATCAGCAGTT TGTAGCCAAT GTGGAAGAGG AGGAAGCCTG GATCAATGAG 
AAAATGACCC TGGTGGCCAG CGAAGATTAT GGCGACACTC TTGCTGCCAT CCAGGGCTTA 
CTGAAGAAAC ATGAAGCTTT TGAGACAGAC TTCACCGTCC ACAAGGATCG CGTGAATGAC 
GTCTGCACCA ATGGACAAGA CCTCATTAAG AAGAACAATC ACCATGAGGA GAACATCTCT 
TCAAAGATGA AGGGCCTGAA CGGGAAAGTG TCAGACCTGG AGAAAGCTGC AGCCCAGAGA 
AAGGCGAAGC TGGATGAGAA CTCGGCCTTC CTTCAGTTCA ACTGGAAGGC GGACGTGGTG 
GAGTCCTGGA TCGGTGAAAA GGAGAACAGC TTGAAGACAG ATGATTATGG CCGAGACCTG 
TCTTCTGTGC AGACGCTCCT CACCAAACAG GAAACTTTTG ACGCTGGGCT GCAGGCCTTC 
CAGCAGGAAG GCATTGCCAA CATCACTGCC CTCAAAGATC AGCTTCTCGC CGCCAAACAC 
GTTCAGTCCA AGGCCATCGA GGCCCGGCAC GCCTCCCTCA TGAAGAGGTG GAGCCAGCTT 
CTGGCCAACT CAGCCGCCCG CAAGAAGAAG CTTCTGGAGG CGCAGAGTCA CTTCCGCAAG 
GTGGAGGACC TCTTCCTGAC CTTTGCCAAA AAGGCTTCTG CCTTCAACAG CTGGTTTGAA 
AATGCAGAGG AGGACTTAAC AGACCCCGTG CGCTGCAACT CCTTGGAAGA AATCAAAGCT 
TTGCGCGAGG CCCACGACGC CTTCCGCTCC TCCCTCAGCT CTGCCCAGGC TGACTTCAAC 
CAGCTGGCCG AGCTGGACCG CCAGATCAAG AGCTTCCGCG TAGCCTCCAA CCCCTACACC 
TGGTTTACCA TGGAGGCCCT GGAGGAGACC TGGAGGAACC TACAGAAAAT CATCAAGGAG 
AGGGAGCTGG AGCTGCAGAA GGAACAGCGG CGGCAGGAGG AGAACGACAA GCTGCGCCAG 
GAGTTTGCCC AGCACGCCAA CGCCTTCCAC CAGTGGATCC AAGAGACCAG GACATACCTC 
CTCGATGGGT CCTGTATGGT GGAAGAGTCG GGGACCCTCG AATCCCAGCT TGAAGCTACC 
AAACGCAAGC ACCAGGAAAT CCGAGCCATG AGAAGTCAGC TCAAAAAGAT CGAGGACCTG 
GGGGCCGCCA TGGAGGAGGC CCTCATCCTG GACAACAAGT ACACGGAGCA CAGCACCGTG 
GGCCTCGCCC AGCAGTGGGA CCAGCTGGAC CAGCTGGGCA TGCGCATGCA GCACAACCTG 
GAGCAGCAGA TCCAGGCCAG GAACACAACA GGTGTGACTG AGGAGGCCCT CAAAGAATTC 
AGCATGATGT TTAAACACTT TGACAAGGAC AAGTCTGGCA GGCTGAACCA TCAGGAGTTC 
AAATCTTGCC TGCGCTCCCT GGGCTATGAC CTGCCCATGG TGGAGGAAGG GGAACCTGAC 
CCTGAGTTCG AGGCAATCCT GGACACGGTG GATCCGAACA GAGATGGCCA TGTCTCCCTG 
CAAGAATACA TGGCTTTCAT GATCAGCCGC GAAACTGAGA ACGTCAAGTC CAGCGAGGAG 
ATTGAGAGCG CCTTCCGGGC CCTCAGCTCA GAGGGAAAGC CTTACGTGAC CAAGGAGGAG 
CTCTACCAGA ACCTGACCCG GGAACAAGCC GACTACTGCG TCTCCCACAT GAAGCCCTAC 
GTGGACGGCA AGGGCCGCGA GCTCCCCACC GCGTTCGACT ACGTGGAGTT CACCCGCTCG 
CTTTTCGTGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016817287.1
CDS	121..7515
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_016961798.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016961798.1

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAATTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAATG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA ACACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCCC TGCTAGAGGC AGATGTGGCT 
GCTCATCAGG ACCGAATTGA TGGCATTACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATGTTGAGGA TGAGGAGACG TGGATTCGAG AGAAGGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCAGCT GCTTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAGGC TCTAAACACA GACAATTATG GACATGATCT TGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAAT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCGTTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAAATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACGGCAAG TGATGAGTCA TACAAGGATC CCACCAACAT CCAGAGCAAG 
CACCAGAAGC ACCAGGCTTT TGAAGCAGAG CTGCATGCCA ACGCTGACCG GATCCGTGGG 
GTTATCGACA TGGGCAACTC CCTCATTGAA CGTGGAGCCT GTGCCGGCAG TGAGGATGCT 
GTCAAGGCCC GCCTGGCTGC CTTAGCTGAC CAGTGGCAAT TCTTGGTGCA AAAGTCAGCG 
GAAAAGAGCC AGAAACTGAA AGAAGCCAAC AAGCAGCAGA ACTTCAACAC AGGGATCAAG 
GACTTTGACT TCTGGCTGTC TGAGGTGGAG GCCCTGCTGG CATCCGAAGA TTATGGCAAA 
GACCTGGCTT CTGTGAACAA CCTGCTGAAA AAGCATCAAC TGCTGGAAGC AGATATATCT 
GCCCATGAGG ATCGCCTGAA GGACCTGAAC AGCCAGGCAG ACAGCCTGAT GACCAGCAGT 
GCCTTCGACA CCTCCCAAGT AAAGGACAAG AGGGACACCA TCAACGGGCG CTTCCAGAAG 
ATCAAGAGCA TGGCGGCCTC CCGGCGAGCC AAGCTGAATG AATCCCATCG CCTGCACCAG 
TTCTTCCGGG ACATGGATGA CGAGGAGTCC TGGATCAAGG AGAAGAAGCT GCTGGTGGGC 
TCGGAGGACT ACGGCCGGGA CCTAACCGGC GTGCAGAACC TGAGGAAGAA GCACAAGCGC 
CTGGAAGCAG AACTGGCTGC GCATGAGCCG GCTATTCAGG GTGTCCTGGA CACTGGCAAG 
AAGCTGTCCG ATGATAACAC CATCGGGAAA GAGGAGATCC AGCAGCGGCT GGCGCAGTTT 
GTGGAGCACT GGAAAGAGCT GAAGCAGCTG GCAGCTGCCC GGGGTCAGCG GCTGGAAGAG 
TCCTTGGAAT ATCAGCAGTT TGTAGCCAAT GTGGAAGAGG AGGAAGCCTG GATCAATGAG 
AAAATGACCC TGGTGGCCAG CGAAGATTAT GGCGACACTC TTGCTGCCAT CCAGGGCTTA 
CTGAAGAAAC ATGAAGCTTT TGAGACAGAC TTCACCGTCC ACAAGGATCG CGTGAATGAC 
GTCTGCACCA ATGGACAAGA CCTCATTAAG AAGAACAATC ACCATGAGGA GAACATCTCT 
TCAAAGATGA AGGGCCTGAA CGGGAAAGTG TCAGACCTGG AGAAAGCTGC AGCCCAGAGA 
AAGGCGAAGC TGGATGAGAA CTCGGCCTTC CTTCAGTTCA ACTGGAAGGC GGACGTGGTG 
GAGTCCTGGA TCGGTGAAAA GGAGAACAGC TTGAAGACAG ATGATTATGG CCGAGACCTG 
TCTTCTGTGC AGACGCTCCT CACCAAACAG GAAACTTTTG ACGCTGGGCT GCAGGCCTTC 
CAGCAGGAAG GCATTGCCAA CATCACTGCC CTCAAAGATC AGCTTCTCGC CGCCAAACAC 
GTTCAGTCCA AGGCCATCGA GGCCCGGCAC GCCTCCCTCA TGAAGAGGTG GAGCCAGCTT 
CTGGCCAACT CAGCCGCCCG CAAGAAGAAG CTTCTGGAGG CGCAGAGTCA CTTCCGCAAG 
GTGGAGGACC TCTTCCTGAC CTTTGCCAAA AAGGCTTCTG CCTTCAACAG CTGGTTTGAA 
AATGCAGAGG AGGACTTAAC AGACCCCGTG CGCTGCAACT CCTTGGAAGA AATCAAAGCT 
TTGCGCGAGG CCCACGACGC CTTCCGCTCC TCCCTCAGCT CTGCCCAGGC TGACTTCAAC 
CAGCTGGCCG AGCTGGACCG CCAGATCAAG AGCTTCCGCG TAGCCTCCAA CCCCTACACC 
TGGTTTACCA TGGAGGCCCT GGAGGAGACC TGGAGGAACC TACAGAAAAT CATCAAGGAG 
AGGGAGCTGG AGCTGCAGAA GGAACAGCGG CGGCAGGAGG AGAACGACAA GCTGCGCCAG 
GAGTTTGCCC AGCACGCCAA CGCCTTCCAC CAGTGGATCC AAGAGACCAG GACATACCTC 
CTCGATGGGT CCTGTATGGT GGAAGAGTCG GGGACCCTCG AATCCCAGCT TGAAGCTACC 
AAACGCAAGC ACCAGGAAAT CCGAGCCATG AGAAGTCAGC TCAAAAAGAT CGAGGACCTG 
GGGGCCGCCA TGGAGGAGGC CCTCATCCTG GACAACAAGT ACACGGAGCA CAGCACCGTG 
GGCCTCGCCC AGCAGTGGGA CCAGCTGGAC CAGCTGGGCA TGCGCATGCA GCACAACCTG 
GAGCAGCAGA TCCAGGCCAG GAACACAACA GGTGTGACTG AGGAGGCCCT CAAAGAATTC 
AGCATGATGT TTAAACACTT TGACAAGGAC AAGTCTGGCA GGCTGAACCA TCAGGAGTTC 
AAATCTTGCC TGCGCTCCCT GGGCTATGAC CTGCCCATGG TGGAGGAAGG GGAACCTGAC 
CCTGAGTTCG AGGCAATCCT GGACACGGTG GATCCGAACA GAGATGGCCA TGTCTCCCTG 
CAAGAATACA TGGCTTTCAT GATCAGCCGC GAAACTGAGA ACGTCAAGTC CAGCGAGGAG 
ATTGAGAGCG CCTTCCGGGC CCTCAGCTCA GAGGGAAAGC CTTACGTGAC CAAGGAGGAG 
CTCTACCAGA ACCTGACCCG GGAACAAGCC GACTACTGCG TCTCCCACAT GAAGCCCTAC 
GTGGACGGCA AGGGCCGCGA GCTCCCCACC GCGTTCGACT ACGTGGAGTT CACCCGCTCG 
CTTTTCGTGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_009457429.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009455704.2 Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X11, mRNA.

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CloneID	OPc89639
Clone ID Related Accession (Same CDS sequence)	XM_009457429.2
Accession Version	XM_009457429.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7359bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006476.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009457429.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAA TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AATGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAACACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCCCTGCTAG AGGCAGATGT GGCTGCTCAT CAGGACCGAA TTGATGGCAT TACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAGG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC AGCTGCTTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AGGCTCTAAA CACAGACAAT 
TATGGACATG ATCTTGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAATCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCGTT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAAATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACGG CAAGTGATGA GTCATACAAG 
GATCCCACCA ACATCCAGAG CAAGCACCAG AAGCACCAGG CTTTTGAAGC AGAGCTGCAT 
GCCAACGCTG ACCGGATCCG TGGGGTTATC GACATGGGCA ACTCCCTCAT TGAACGTGGA 
GCCTGTGCCG GCAGTGAGGA TGCTGTCAAG GCCCGCCTGG CTGCCTTAGC TGACCAGTGG 
CAATTCTTGG TGCAAAAGTC AGCGGAAAAG AGCCAGAAAC TGAAAGAAGC CAACAAGCAG 
CAGAACTTCA ACACAGGGAT CAAGGACTTT GACTTCTGGC TGTCTGAGGT GGAGGCCCTG 
CTGGCATCCG AAGATTATGG CAAAGACCTG GCTTCTGTGA ACAACCTGCT GAAAAAGCAT 
CAACTGCTGG AAGCAGATAT ATCTGCCCAT GAGGATCGCC TGAAGGACCT GAACAGCCAG 
GCAGACAGCC TGATGACCAG CAGTGCCTTC GACACCTCCC AAGTAAAGGA CAAGAGGGAC 
ACCATCAACG GGCGCTTCCA GAAGATCAAG AGCATGGCGG CCTCCCGGCG AGCCAAGCTG 
AATGAATCCC ATCGCCTGCA CCAGTTCTTC CGGGACATGG ATGACGAGGA GTCCTGGATC 
AAGGAGAAGA AGCTGCTGGT GGGCTCGGAG GACTACGGCC GGGACCTAAC CGGCGTGCAG 
AACCTGAGGA AGAAGCACAA GCGCCTGGAA GCAGAACTGG CTGCGCATGA GCCGGCTATT 
CAGGGTGTCC TGGACACTGG CAAGAAGCTG TCCGATGATA ACACCATCGG GAAAGAGGAG 
ATCCAGCAGC GGCTGGCGCA GTTTGTGGAG CACTGGAAAG AGCTGAAGCA GCTGGCAGCT 
GCCCGGGGTC AGCGGCTGGA AGAGTCCTTG GAATATCAGC AGTTTGTAGC CAATGTGGAA 
GAGGAGGAAG CCTGGATCAA TGAGAAAATG ACCCTGGTGG CCAGCGAAGA TTATGGCGAC 
ACTCTTGCTG CCATCCAGGG CTTACTGAAG AAACATGAAG CTTTTGAGAC AGACTTCACC 
GTCCACAAGG ATCGCGTGAA TGACGTCTGC ACCAATGGAC AAGACCTCAT TAAGAAGAAC 
AATCACCATG AGGAGAACAT CTCTTCAAAG ATGAAGGGCC TGAACGGGAA AGTGTCAGAC 
CTGGAGAAAG CTGCAGCCCA GAGAAAGGCG AAGCTGGATG AGAACTCGGC CTTCCTTCAG 
TTCAACTGGA AGGCGGACGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG 
ACAGATGATT ATGGCCGAGA CCTGTCTTCT GTGCAGACGC TCCTCACCAA ACAGGAAACT 
TTTGACGCTG GGCTGCAGGC CTTCCAGCAG GAAGGCATTG CCAACATCAC TGCCCTCAAA 
GATCAGCTTC TCGCCGCCAA ACACGTTCAG TCCAAGGCCA TCGAGGCCCG GCACGCCTCC 
CTCATGAAGA GGTGGAGCCA GCTTCTGGCC AACTCAGCCG CCCGCAAGAA GAAGCTTCTG 
GAGGCGCAGA GTCACTTCCG CAAGGTGGAG GACCTCTTCC TGACCTTTGC CAAAAAGGCT 
TCTGCCTTCA ACAGCTGGTT TGAAAATGCA GAGGAGGACT TAACAGACCC CGTGCGCTGC 
AACTCCTTGG AAGAAATCAA AGCTTTGCGC GAGGCCCACG ACGCCTTCCG CTCCTCCCTC 
AGCTCTGCCC AGGCTGACTT CAACCAGCTG GCCGAGCTGG ACCGCCAGAT CAAGAGCTTC 
CGCGTAGCCT CCAACCCCTA CACCTGGTTT ACCATGGAGG CCCTGGAGGA GACCTGGAGG 
AACCTACAGA AAATCATCAA GGAGAGGGAG CTGGAGCTGC AGAAGGAACA GCGGCGGCAG 
GAGGAGAACG ACAAGCTGCG CCAGGAGTTT GCCCAGCACG CCAACGCCTT CCACCAGTGG 
ATCCAAGAGA CCAGGACATA CCTCCTCGAT GGGTCCTGTA TGGTGGAAGA GTCGGGGACC 
CTCGAATCCC AGCTTGAAGC TACCAAACGC AAGCACCAGG AAATCCGAGC CATGAGAAGT 
CAGCTCAAAA AGATCGAGGA CCTGGGGGCC GCCATGGAGG AGGCCCTCAT CCTGGACAAC 
AAGTACACGG AGCACAGCAC CGTGGGCCTC GCCCAGCAGT GGGACCAGCT GGACCAGCTG 
GGCATGCGCA TGCAGCACAA CCTGGAGCAG CAGATCCAGG CCAGGAACAC AACAGGTGTG 
ACTGAGGAGG CCCTCAAAGA ATTCAGCATG ATGTTTAAAC ACTTTGACAA GGACAAGTCT 
GGCAGGCTGA ACCATCAGGA GTTCAAATCT TGCCTGCGCT CCCTGGGCTA TGACCTGCCC 
ATGGTGGAGG AAGGGGAACC TGACCCTGAG TTCGAGGCAA TCCTGGACAC GGTGGATCCG 
AACAGAGATG GCCATGTCTC CCTGCAAGAA TACATGGCTT TCATGATCAG CCGCGAAACT 
GAGAACGTCA AGTCCAGCGA GGAGATTGAG AGCGCCTTCC GGGCCCTCAG CTCAGAGGGA 
AAGCCTTACG TGACCAAGGA GGAGCTCTAC CAGAACCTGA CCCGGGAACA AGCCGACTAC 
TGCGTCTCCC ACATGAAGCC CTACGTGGAC GGCAAGGGCC GCGAGCTCCC CACCGCGTTC 
GACTACGTGG AGTTCACCCG CTCGCTTTTC GTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009455704.2
CDS	137..7495
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_009457429.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009457429.2

ORF Insert Sequence:

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAA TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AATGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAACACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCCCTGCTAG AGGCAGATGT GGCTGCTCAT CAGGACCGAA TTGATGGCAT TACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAGG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC AGCTGCTTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AGGCTCTAAA CACAGACAAT 
TATGGACATG ATCTTGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAATCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCGTT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAAATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACGG CAAGTGATGA GTCATACAAG 
GATCCCACCA ACATCCAGAG CAAGCACCAG AAGCACCAGG CTTTTGAAGC AGAGCTGCAT 
GCCAACGCTG ACCGGATCCG TGGGGTTATC GACATGGGCA ACTCCCTCAT TGAACGTGGA 
GCCTGTGCCG GCAGTGAGGA TGCTGTCAAG GCCCGCCTGG CTGCCTTAGC TGACCAGTGG 
CAATTCTTGG TGCAAAAGTC AGCGGAAAAG AGCCAGAAAC TGAAAGAAGC CAACAAGCAG 
CAGAACTTCA ACACAGGGAT CAAGGACTTT GACTTCTGGC TGTCTGAGGT GGAGGCCCTG 
CTGGCATCCG AAGATTATGG CAAAGACCTG GCTTCTGTGA ACAACCTGCT GAAAAAGCAT 
CAACTGCTGG AAGCAGATAT ATCTGCCCAT GAGGATCGCC TGAAGGACCT GAACAGCCAG 
GCAGACAGCC TGATGACCAG CAGTGCCTTC GACACCTCCC AAGTAAAGGA CAAGAGGGAC 
ACCATCAACG GGCGCTTCCA GAAGATCAAG AGCATGGCGG CCTCCCGGCG AGCCAAGCTG 
AATGAATCCC ATCGCCTGCA CCAGTTCTTC CGGGACATGG ATGACGAGGA GTCCTGGATC 
AAGGAGAAGA AGCTGCTGGT GGGCTCGGAG GACTACGGCC GGGACCTAAC CGGCGTGCAG 
AACCTGAGGA AGAAGCACAA GCGCCTGGAA GCAGAACTGG CTGCGCATGA GCCGGCTATT 
CAGGGTGTCC TGGACACTGG CAAGAAGCTG TCCGATGATA ACACCATCGG GAAAGAGGAG 
ATCCAGCAGC GGCTGGCGCA GTTTGTGGAG CACTGGAAAG AGCTGAAGCA GCTGGCAGCT 
GCCCGGGGTC AGCGGCTGGA AGAGTCCTTG GAATATCAGC AGTTTGTAGC CAATGTGGAA 
GAGGAGGAAG CCTGGATCAA TGAGAAAATG ACCCTGGTGG CCAGCGAAGA TTATGGCGAC 
ACTCTTGCTG CCATCCAGGG CTTACTGAAG AAACATGAAG CTTTTGAGAC AGACTTCACC 
GTCCACAAGG ATCGCGTGAA TGACGTCTGC ACCAATGGAC AAGACCTCAT TAAGAAGAAC 
AATCACCATG AGGAGAACAT CTCTTCAAAG ATGAAGGGCC TGAACGGGAA AGTGTCAGAC 
CTGGAGAAAG CTGCAGCCCA GAGAAAGGCG AAGCTGGATG AGAACTCGGC CTTCCTTCAG 
TTCAACTGGA AGGCGGACGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG 
ACAGATGATT ATGGCCGAGA CCTGTCTTCT GTGCAGACGC TCCTCACCAA ACAGGAAACT 
TTTGACGCTG GGCTGCAGGC CTTCCAGCAG GAAGGCATTG CCAACATCAC TGCCCTCAAA 
GATCAGCTTC TCGCCGCCAA ACACGTTCAG TCCAAGGCCA TCGAGGCCCG GCACGCCTCC 
CTCATGAAGA GGTGGAGCCA GCTTCTGGCC AACTCAGCCG CCCGCAAGAA GAAGCTTCTG 
GAGGCGCAGA GTCACTTCCG CAAGGTGGAG GACCTCTTCC TGACCTTTGC CAAAAAGGCT 
TCTGCCTTCA ACAGCTGGTT TGAAAATGCA GAGGAGGACT TAACAGACCC CGTGCGCTGC 
AACTCCTTGG AAGAAATCAA AGCTTTGCGC GAGGCCCACG ACGCCTTCCG CTCCTCCCTC 
AGCTCTGCCC AGGCTGACTT CAACCAGCTG GCCGAGCTGG ACCGCCAGAT CAAGAGCTTC 
CGCGTAGCCT CCAACCCCTA CACCTGGTTT ACCATGGAGG CCCTGGAGGA GACCTGGAGG 
AACCTACAGA AAATCATCAA GGAGAGGGAG CTGGAGCTGC AGAAGGAACA GCGGCGGCAG 
GAGGAGAACG ACAAGCTGCG CCAGGAGTTT GCCCAGCACG CCAACGCCTT CCACCAGTGG 
ATCCAAGAGA CCAGGACATA CCTCCTCGAT GGGTCCTGTA TGGTGGAAGA GTCGGGGACC 
CTCGAATCCC AGCTTGAAGC TACCAAACGC AAGCACCAGG AAATCCGAGC CATGAGAAGT 
CAGCTCAAAA AGATCGAGGA CCTGGGGGCC GCCATGGAGG AGGCCCTCAT CCTGGACAAC 
AAGTACACGG AGCACAGCAC CGTGGGCCTC GCCCAGCAGT GGGACCAGCT GGACCAGCTG 
GGCATGCGCA TGCAGCACAA CCTGGAGCAG CAGATCCAGG CCAGGAACAC AACAGGTGTG 
ACTGAGGAGG CCCTCAAAGA ATTCAGCATG ATGTTTAAAC ACTTTGACAA GGACAAGTCT 
GGCAGGCTGA ACCATCAGGA GTTCAAATCT TGCCTGCGCT CCCTGGGCTA TGACCTGCCC 
ATGGTGGAGG AAGGGGAACC TGACCCTGAG TTCGAGGCAA TCCTGGACAC GGTGGATCCG 
AACAGAGATG GCCATGTCTC CCTGCAAGAA TACATGGCTT TCATGATCAG CCGCGAAACT 
GAGAACGTCA AGTCCAGCGA GGAGATTGAG AGCGCCTTCC GGGCCCTCAG CTCAGAGGGA 
AAGCCTTACG TGACCAAGGA GGAGCTCTAC CAGAACCTGA CCCGGGAACA AGCCGACTAC 
TGCGTCTCCC ACATGAAGCC CTACGTGGAC GGCAAGGGCC GCGAGCTCCC CACCGCGTTC 
GACTACGTGG AGTTCACCCG CTCGCTTTTC GTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_016961799.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016817288.1 Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X12, mRNA.

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CloneID	OPc89640
Clone ID Related Accession (Same CDS sequence)	XM_016961799.1
Accession Version	XM_016961799.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7533bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006476.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAATTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAATG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA ACACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCCC TGCTAGAGGC AGATGTGGCT 
GCTCATCAGG ACCGAATTGA TGGCATTACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATGTTGAGGA TGAGGAGACG TGGATTCGAG AGAAGGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCAGCT GCTTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AGACACGCAT AACTAAGGAG GCCGGCAGTG TATCTCTGCG TATGAAGCAG 
GTGGAAGAAC TATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAGGC TCTAAACACA GACAATTATG GACATGATCT TGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAAT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCGTTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAAATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACGGCAAG TGATGAGTCA TACAAGGATC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCACCA GAAGCACCAG GCTTTTGAAG CAGAGCTGCA TGCCAACGCT 
GACCGGATCC GTGGGGTTAT CGACATGGGC AACTCCCTCA TTGAACGTGG AGCCTGTGCC 
GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCTTAG CTGACCAGTG GCAATTCTTG 
GTGCAAAAGT CAGCGGAAAA GAGCCAGAAA CTGAAAGAAG CCAACAAGCA GCAGAACTTC 
AACACAGGGA TCAAGGACTT TGACTTCTGG CTGTCTGAGG TGGAGGCCCT GCTGGCATCC 
GAAGATTATG GCAAAGACCT GGCTTCTGTG AACAACCTGC TGAAAAAGCA TCAACTGCTG 
GAAGCAGATA TATCTGCCCA TGAGGATCGC CTGAAGGACC TGAACAGCCA GGCAGACAGC 
CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTAAAGG ACAAGAGGGA CACCATCAAC 
GGGCGCTTCC AGAAGATCAA GAGCATGGCG GCCTCCCGGC GAGCCAAGCT GAATGAATCC 
CATCGCCTGC ACCAGTTCTT CCGGGACATG GATGACGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGGGCTCGGA GGACTACGGC CGGGACCTAA CCGGCGTGCA GAACCTGAGG 
AAGAAGCACA AGCGCCTGGA AGCAGAACTG GCTGCGCATG AGCCGGCTAT TCAGGGTGTC 
CTGGACACTG GCAAGAAGCT GTCCGATGAT AACACCATCG GGAAAGAGGA GATCCAGCAG 
CGGCTGGCGC AGTTTGTGGA GCACTGGAAA GAGCTGAAGC AGCTGGCAGC TGCCCGGGGT 
CAGCGGCTGG AAGAGTCCTT GGAATATCAG CAGTTTGTAG CCAATGTGGA AGAGGAGGAA 
GCCTGGATCA ATGAGAAAAT GACCCTGGTG GCCAGCGAAG ATTATGGCGA CACTCTTGCT 
GCCATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC CGTCCACAAG 
GATCGCGTGA ATGACGTCTG CACCAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCAAA GATGAAGGGC CTGAACGGGA AAGTGTCAGA CCTGGAGAAA 
GCTGCAGCCC AGAGAAAGGC GAAGCTGGAT GAGAACTCGG CCTTCCTTCA GTTCAACTGG 
AAGGCGGACG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGCCGAG ACCTGTCTTC TGTGCAGACG CTCCTCACCA AACAGGAAAC TTTTGACGCT 
GGGCTGCAGG CCTTCCAGCA GGAAGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTT 
CTCGCCGCCA AACACGTTCA GTCCAAGGCC ATCGAGGCCC GGCACGCCTC CCTCATGAAG 
AGGTGGAGCC AGCTTCTGGC CAACTCAGCC GCCCGCAAGA AGAAGCTTCT GGAGGCGCAG 
AGTCACTTCC GCAAGGTGGA GGACCTCTTC CTGACCTTTG CCAAAAAGGC TTCTGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CCGTGCGCTG CAACTCCTTG 
GAAGAAATCA AAGCTTTGCG CGAGGCCCAC GACGCCTTCC GCTCCTCCCT CAGCTCTGCC 
CAGGCTGACT TCAACCAGCT GGCCGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTAGCC 
TCCAACCCCT ACACCTGGTT TACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTACAG 
AAAATCATCA AGGAGAGGGA GCTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT TGCCCAGCAC GCCAACGCCT TCCACCAGTG GATCCAAGAG 
ACCAGGACAT ACCTCCTCGA TGGCATAGCA TATCGTCGGG TCATTCGTGT CTATCAGTAT 
GAAGTTGGGG ATGATCTGTC TGGAAGGTCC TGTATGGTGG AAGAGTCGGG GACCCTCGAA 
TCCCAGCTTG AAGCTACCAA ACGCAAGCAC CAGGAAATCC GAGCCATGAG AAGTCAGCTC 
AAAAAGATCG AGGACCTGGG GGCCGCCATG GAGGAGGCCC TCATCCTGGA CAACAAGTAC 
ACGGAGCACA GCACCGTGGG CCTCGCCCAG CAGTGGGACC AGCTGGACCA GCTGGGCATG 
CGCATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACAACAGG TGTGACTGAG 
GAGGCCCTCA AAGAATTCAG CATGATGTTT AAACACTTTG ACAAGGACAA GTCTGGCAGG 
CTGAACCATC AGGAGTTCAA ATCTTGCCTG CGCTCCCTGG GCTATGACCT GCCCATGGTG 
GAGGAAGGGG AACCTGACCC TGAGTTCGAG GCAATCCTGG ACACGGTGGA TCCGAACAGA 
GATGGCCATG TCTCCCTGCA AGAATACATG GCTTTCATGA TCAGCCGCGA AACTGAGAAC 
GTCAAGTCCA GCGAGGAGAT TGAGAGCGCC TTCCGGGCCC TCAGCTCAGA GGGAAAGCCT 
TACGTGACCA AGGAGGAGCT CTACCAGAAC CTGACCCGGG AACAAGCCGA CTACTGCGTC 
TCCCACATGA AGCCCTACGT GGACGGCAAG GGCCGCGAGC TCCCCACCGC GTTCGACTAC 
GTGGAGTTCA CCCGCTCGCT TTTCGTGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016817288.1
CDS	121..7653
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRITKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_016961799.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016961799.1

ORF Insert Sequence:

ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACCG ATACCACCGC 
TTCAAGGAAC TCTCAACCCT TAGGCGTCAG AAGCTGGAAG ATTCCTATCG ATTCCAGTTC 
TTTCAAAGAG ATGCTGAAGA GCTGGAGAAA TGGATACAGG AAAAACTTCA GATTGCATCT 
GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGAAAGC TTCAGAAGCA TCAAGCATTT 
GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAGC TGGATGAAAC TGGAAACCTG 
ATGATCTCAG AAGGGCATTT TGCATCTGAA ACCATACGGA CCCGTTTGAT GGAGCTGCAC 
CGCCAGTGGG AATTACTTTT GGAGAAGATG CGAGAAAAAG GAATCAAACT GCTGCAGGCC 
CAGAAGTTGG TGCAGTACTT ACGAGAATGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCAATTG TTACTTCTGA AGAGCTGGGC CAGGATCTGG AGCATGTAGA GGTTTTACAG 
AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCTCATG AAGAAAGAGT TAATGAAGTG 
AACCAATTTG CTGCCAAACT CATACAGGAG CAGCACCCTG AGGAGGAACT GATCAAGACT 
AAGCAGGATG AAGTCAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCTCT GCAGAGGCAG 
GGGAAGCTCT TTGGGGCAGC AGAAGTTCAG CGCTTTAACA GGGATGTGGA TGAGACTATC 
AGTTGGATTA AGGAAAAGGA GCAGTTAATG GCCTCTGATG ATTTTGGCCG AGACCTGGCA 
AGTGTTCAGG CTCTGCTTCG GAAGCACGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAA 
GACAAGGTCA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCCCA CCCTCTGAGT 
GCAACACAGA TTCAAGTGAA GCGAGAGGAA CTGATTACAA ACTGGGAGCA GATCCGCACC 
TTGGCGGCAG AGAGACATGC ACGGCTCAAT GATTCATACA GGCTTCAACG CTTCCTTGCT 
GACTTCCGTG ACCTCACCAG CTGGGTGACT GAGATGAAAG CCCTCATCAA TGCAGATGAG 
CTTGCCAATG ATGTGGCTGG GGCTGAAGCC CTGCTAGATA GACACCAAGA ACACAAGGGT 
GAAATTGATG CCCATGAAGA CAGCTTCAAA TCTGCAGATG AATCTGGACA GGCACTGCTT 
GCTGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCGTCCT TTCCGAGGAG 
AGAGCGGCGC TGCTGGAGCT GTGGGAGCTG CGCAGGCAGC AGTACGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGGGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ATGAAGACTT GGGAGATTCC TTGGATAGTG TGGAAGCGCT TCTTAAGAAG 
CACGAAGACT TTGAGAAATC CCTTAGTGCC CAGGAGGAAA AGATTACAGC ATTAGATGAA 
TTTGCAACCA AGCTAATTCA GAACAACCAC TATGCAATGG AAGATGTGGC CACTCGCCGA 
GATGCTCTGT TGAGCCGCCG CAATGCCCTT CACGAGAGAG CCATGCGTCG CCGGGCCCAG 
CTAGCCGATT CTTTCCATCT GCAGCAGTTT TTCCGTGATT CTGATGAGCT CAAGAGTTGG 
GTCAATGAGA AGATGAAAAC TGCCACAGAT GAAGCTTATA AAGATCCATC CAACCTACAA 
GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CAGCAAACCA GAGCCGAATT 
GATGCCTTGG AGAAAGCTGG CCAAAAGCTG ATTGATGTCA ACCACTATGC CAAGGATGAA 
GTGGCAGCTC GTATGAATGA GGTGATCAGT TTGTGGAAGA AACTGCTAGA GGCCACTGAA 
CTGAAAGGAA TAAAGCTTCG TGAAGCCAAC CAGCAACAGC AATTTAATCG CAATGTTGAG 
GATATTGAAT TGTGGCTATA TGAAGTAGAA GGTCACTTGG CTTCGGATGA TTACGGCAAA 
GATCTTACCA ATGTGCAGAA CCTCCAGAAG AAACATGCCC TGCTAGAGGC AGATGTGGCT 
GCTCATCAGG ACCGAATTGA TGGCATTACC ATTCAGGCCC GCCAGTTCCA AGATGCTGGC 
CATTTTGATG CAGAAAACAT CAAGAAGAAA CAGGAAGCCC TCGTGGCTCG CTATGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCTCTGCG GTTGCAGCAG 
CTCTTCCGGG ATGTTGAGGA TGAGGAGACG TGGATTCGAG AGAAGGAGCC CATTGCCGCA 
TCTACCAACA GAGGTAAGGA TTTAATTGGG GTCCAGAATC TGCTAAAGAA ACATCAAGCC 
TTACAAGCAG AAATTGCTGG ACATGAACCA CGCATCAAAG CAGTTACACA GAAGGGGAAT 
GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAGCT TCACGAGCTG 
AACCAAAAGT GGGAGGCACT GAAAGCCAAA GCTTCCCAGC GTCGGCAGGA CCTGGAGGAC 
TCTCTGCAGG CCCAGCAGTA CTTTGCTGAT GCTAACGAGG CTGAATCCTG GATGCGGGAG 
AAGGAACCCA TTGTGGGCAG CACTGACTAT GGCAAGGACG AAGACTCTGC TGAGGCTCTA 
CTGAAGAAAC ACGAAGCTTT GATGTCAGAT CTCAGTGCCT ACGGCAGCAG CATCCAGGCT 
TTGCGAGAAC AAGCACAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG 
AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAGGGAG ATATCCTTAC CTTACTCAAC AGCACCAACA AGGATTGGTG GAAAGTGGAA 
GTGAACGATC GTCAGGGTTT TGTGCCAGCT GCTTACGTGA AGAAATTGGA CCCCGCCCAG 
TCAGCCTCCC GGGAGAATCT CCTGGAGGAG CAAGGCAGCA TAGCACTGCG GCAGGAGCAG 
ATTGACAATC AGACACGCAT AACTAAGGAG GCCGGCAGTG TATCTCTGCG TATGAAGCAG 
GTGGAAGAAC TATATCATTC TCTGCTGGAA CTGGGTGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGTTGCA AGAAGTTTAT GTTGTTCCGT GAAGCGAATG AACTACAGCA ATGGATCAAT 
GAGAAGGAAG CCGCTCTGAC AAGTGAGGAG GTCGGAGCAG ACTTGGAGCA GGTTGAGGTG 
CTCCAGAAGA AGTTTGATGA CTTCCAGAAG GACCTGAAGG CCAATGAGTC ACGGTTGAAG 
GACATTAACA AGGTAGCTGA AGACCTGGAG TCTGAAGGTC TCATGGCAGA GGAGGTGCAG 
GCTGTGCAAC AACAGGAAGT GTATGGCATG ATGCCCAGGG ATGAAACTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGTCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTGA ATGAGCGCTG GCGGTCCCTA CAGCAGCTGG CCGAGGAACG GAGCCAGCTC 
TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CTGATGAAAC CAAAGAATGG 
ATTGAAGAGA AGAATCAGGC TCTAAACACA GACAATTATG GACATGATCT TGCCAGTGTC 
CAGGCCCTGC AACGCAAGCA TGAGGGCTTC GAGAGGGACC TTGCGGCTCT CGGTGACAAG 
GTAAACTCCC TTGGTGAAAC AGCAGAGCGC CTGATCCAAT CCCATCCCGA GTCAGCAGAA 
GACCTGCAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA GCAGCCTGGG GAAACGTGCA 
GATCAGCGCA AGGCAAAGTT GGGTGACTCC CACGACCTGC AGCGCTTCCT TAGCGATTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATA CGGGGGTTGG TGTCCTCAGA TGAGCTAGCC 
AAGGATGTCA CCGGAGCTGA GGCGTTGCTG GAGCGACACC AGGAACACCG GACAGAAATC 
GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GACAGCAGCT GTTGGCTCAC 
GGACACTATG CCAGCCCTGA GATCAAGCAG AAACTTGATA TTCTTGACCA GGAGCGTGCA 
GACCTGGAGA AGGCCTGGGT TCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAACTGCAG 
CTGTTCCATC GGGACTGTGA GCAAGCTGAG AACTGGATGG CTGCCCGGGA GGCCTTCTTG 
AATACCGAAG ACAAAGGAGA CTCACTGGAC AGCGTAGAGG CTCTGATCAA AAAACATGAA 
GACTTTGACA AAGCGATTAA CGTCCAGGAA GAGAAGATTG CTGCTCTGCA GGCCTTTGCC 
GACCAGCTCA TCGCTGCCGG CCATTATGCC AAGGGAGACA TTTCTAGCCG GCGCAATGAG 
GTCTTGGACA GGTGGCGACG TCTGAAAGCC CAAATGATTG AGAAAAGGTC AAAGCTAGGA 
GAATCTCAAA CCCTCCAACA GTTCAGCCGG GATGTGGATG AGATTGAGGC TTGGATCAGT 
GAAAAATTGC AAACGGCAAG TGATGAGTCA TACAAGGATC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCACCA GAAGCACCAG GCTTTTGAAG CAGAGCTGCA TGCCAACGCT 
GACCGGATCC GTGGGGTTAT CGACATGGGC AACTCCCTCA TTGAACGTGG AGCCTGTGCC 
GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCTTAG CTGACCAGTG GCAATTCTTG 
GTGCAAAAGT CAGCGGAAAA GAGCCAGAAA CTGAAAGAAG CCAACAAGCA GCAGAACTTC 
AACACAGGGA TCAAGGACTT TGACTTCTGG CTGTCTGAGG TGGAGGCCCT GCTGGCATCC 
GAAGATTATG GCAAAGACCT GGCTTCTGTG AACAACCTGC TGAAAAAGCA TCAACTGCTG 
GAAGCAGATA TATCTGCCCA TGAGGATCGC CTGAAGGACC TGAACAGCCA GGCAGACAGC 
CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTAAAGG ACAAGAGGGA CACCATCAAC 
GGGCGCTTCC AGAAGATCAA GAGCATGGCG GCCTCCCGGC GAGCCAAGCT GAATGAATCC 
CATCGCCTGC ACCAGTTCTT CCGGGACATG GATGACGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGGGCTCGGA GGACTACGGC CGGGACCTAA CCGGCGTGCA GAACCTGAGG 
AAGAAGCACA AGCGCCTGGA AGCAGAACTG GCTGCGCATG AGCCGGCTAT TCAGGGTGTC 
CTGGACACTG GCAAGAAGCT GTCCGATGAT AACACCATCG GGAAAGAGGA GATCCAGCAG 
CGGCTGGCGC AGTTTGTGGA GCACTGGAAA GAGCTGAAGC AGCTGGCAGC TGCCCGGGGT 
CAGCGGCTGG AAGAGTCCTT GGAATATCAG CAGTTTGTAG CCAATGTGGA AGAGGAGGAA 
GCCTGGATCA ATGAGAAAAT GACCCTGGTG GCCAGCGAAG ATTATGGCGA CACTCTTGCT 
GCCATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC CGTCCACAAG 
GATCGCGTGA ATGACGTCTG CACCAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCAAA GATGAAGGGC CTGAACGGGA AAGTGTCAGA CCTGGAGAAA 
GCTGCAGCCC AGAGAAAGGC GAAGCTGGAT GAGAACTCGG CCTTCCTTCA GTTCAACTGG 
AAGGCGGACG TGGTGGAGTC CTGGATCGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGCCGAG ACCTGTCTTC TGTGCAGACG CTCCTCACCA AACAGGAAAC TTTTGACGCT 
GGGCTGCAGG CCTTCCAGCA GGAAGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTT 
CTCGCCGCCA AACACGTTCA GTCCAAGGCC ATCGAGGCCC GGCACGCCTC CCTCATGAAG 
AGGTGGAGCC AGCTTCTGGC CAACTCAGCC GCCCGCAAGA AGAAGCTTCT GGAGGCGCAG 
AGTCACTTCC GCAAGGTGGA GGACCTCTTC CTGACCTTTG CCAAAAAGGC TTCTGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CCGTGCGCTG CAACTCCTTG 
GAAGAAATCA AAGCTTTGCG CGAGGCCCAC GACGCCTTCC GCTCCTCCCT CAGCTCTGCC 
CAGGCTGACT TCAACCAGCT GGCCGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTAGCC 
TCCAACCCCT ACACCTGGTT TACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTACAG 
AAAATCATCA AGGAGAGGGA GCTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT TGCCCAGCAC GCCAACGCCT TCCACCAGTG GATCCAAGAG 
ACCAGGACAT ACCTCCTCGA TGGCATAGCA TATCGTCGGG TCATTCGTGT CTATCAGTAT 
GAAGTTGGGG ATGATCTGTC TGGAAGGTCC TGTATGGTGG AAGAGTCGGG GACCCTCGAA 
TCCCAGCTTG AAGCTACCAA ACGCAAGCAC CAGGAAATCC GAGCCATGAG AAGTCAGCTC 
AAAAAGATCG AGGACCTGGG GGCCGCCATG GAGGAGGCCC TCATCCTGGA CAACAAGTAC 
ACGGAGCACA GCACCGTGGG CCTCGCCCAG CAGTGGGACC AGCTGGACCA GCTGGGCATG 
CGCATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACAACAGG TGTGACTGAG 
GAGGCCCTCA AAGAATTCAG CATGATGTTT AAACACTTTG ACAAGGACAA GTCTGGCAGG 
CTGAACCATC AGGAGTTCAA ATCTTGCCTG CGCTCCCTGG GCTATGACCT GCCCATGGTG 
GAGGAAGGGG AACCTGACCC TGAGTTCGAG GCAATCCTGG ACACGGTGGA TCCGAACAGA 
GATGGCCATG TCTCCCTGCA AGAATACATG GCTTTCATGA TCAGCCGCGA AACTGAGAAC 
GTCAAGTCCA GCGAGGAGAT TGAGAGCGCC TTCCGGGCCC TCAGCTCAGA GGGAAAGCCT 
TACGTGACCA AGGAGGAGCT CTACCAGAAC CTGACCCGGG AACAAGCCGA CTACTGCGTC 
TCCCACATGA AGCCCTACGT GGACGGCAAG GGCCGCGAGC TCCCCACCGC GTTCGACTAC 
GTGGAGTTCA CCCGCTCGCT TTTCGTGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_009457426.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009455701.2 Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X13, mRNA.

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CloneID	OPc89641
Clone ID Related Accession (Same CDS sequence)	XM_009457426.2
Accession Version	XM_009457426.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7419bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006476.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009457426.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAA TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AATGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAACACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCCCTGCTAG AGGCAGATGT GGCTGCTCAT CAGGACCGAA TTGATGGCAT TACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAGG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC AGCTGCTTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAGACAC GCATAACTAA GGAGGCCGGC 
AGTGTATCTC TGCGTATGAA GCAGGTGGAA GAACTATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AGGCTCTAAA CACAGACAAT 
TATGGACATG ATCTTGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAATCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCGTT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAAATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACGG CAAGTGATGA GTCATACAAG 
GATCCCACCA ACATCCAGAG CAAGCACCAG AAGCACCAGG CTTTTGAAGC AGAGCTGCAT 
GCCAACGCTG ACCGGATCCG TGGGGTTATC GACATGGGCA ACTCCCTCAT TGAACGTGGA 
GCCTGTGCCG GCAGTGAGGA TGCTGTCAAG GCCCGCCTGG CTGCCTTAGC TGACCAGTGG 
CAATTCTTGG TGCAAAAGTC AGCGGAAAAG AGCCAGAAAC TGAAAGAAGC CAACAAGCAG 
CAGAACTTCA ACACAGGGAT CAAGGACTTT GACTTCTGGC TGTCTGAGGT GGAGGCCCTG 
CTGGCATCCG AAGATTATGG CAAAGACCTG GCTTCTGTGA ACAACCTGCT GAAAAAGCAT 
CAACTGCTGG AAGCAGATAT ATCTGCCCAT GAGGATCGCC TGAAGGACCT GAACAGCCAG 
GCAGACAGCC TGATGACCAG CAGTGCCTTC GACACCTCCC AAGTAAAGGA CAAGAGGGAC 
ACCATCAACG GGCGCTTCCA GAAGATCAAG AGCATGGCGG CCTCCCGGCG AGCCAAGCTG 
AATGAATCCC ATCGCCTGCA CCAGTTCTTC CGGGACATGG ATGACGAGGA GTCCTGGATC 
AAGGAGAAGA AGCTGCTGGT GGGCTCGGAG GACTACGGCC GGGACCTAAC CGGCGTGCAG 
AACCTGAGGA AGAAGCACAA GCGCCTGGAA GCAGAACTGG CTGCGCATGA GCCGGCTATT 
CAGGGTGTCC TGGACACTGG CAAGAAGCTG TCCGATGATA ACACCATCGG GAAAGAGGAG 
ATCCAGCAGC GGCTGGCGCA GTTTGTGGAG CACTGGAAAG AGCTGAAGCA GCTGGCAGCT 
GCCCGGGGTC AGCGGCTGGA AGAGTCCTTG GAATATCAGC AGTTTGTAGC CAATGTGGAA 
GAGGAGGAAG CCTGGATCAA TGAGAAAATG ACCCTGGTGG CCAGCGAAGA TTATGGCGAC 
ACTCTTGCTG CCATCCAGGG CTTACTGAAG AAACATGAAG CTTTTGAGAC AGACTTCACC 
GTCCACAAGG ATCGCGTGAA TGACGTCTGC ACCAATGGAC AAGACCTCAT TAAGAAGAAC 
AATCACCATG AGGAGAACAT CTCTTCAAAG ATGAAGGGCC TGAACGGGAA AGTGTCAGAC 
CTGGAGAAAG CTGCAGCCCA GAGAAAGGCG AAGCTGGATG AGAACTCGGC CTTCCTTCAG 
TTCAACTGGA AGGCGGACGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG 
ACAGATGATT ATGGCCGAGA CCTGTCTTCT GTGCAGACGC TCCTCACCAA ACAGGAAACT 
TTTGACGCTG GGCTGCAGGC CTTCCAGCAG GAAGGCATTG CCAACATCAC TGCCCTCAAA 
GATCAGCTTC TCGCCGCCAA ACACGTTCAG TCCAAGGCCA TCGAGGCCCG GCACGCCTCC 
CTCATGAAGA GGTGGAGCCA GCTTCTGGCC AACTCAGCCG CCCGCAAGAA GAAGCTTCTG 
GAGGCGCAGA GTCACTTCCG CAAGGTGGAG GACCTCTTCC TGACCTTTGC CAAAAAGGCT 
TCTGCCTTCA ACAGCTGGTT TGAAAATGCA GAGGAGGACT TAACAGACCC CGTGCGCTGC 
AACTCCTTGG AAGAAATCAA AGCTTTGCGC GAGGCCCACG ACGCCTTCCG CTCCTCCCTC 
AGCTCTGCCC AGGCTGACTT CAACCAGCTG GCCGAGCTGG ACCGCCAGAT CAAGAGCTTC 
CGCGTAGCCT CCAACCCCTA CACCTGGTTT ACCATGGAGG CCCTGGAGGA GACCTGGAGG 
AACCTACAGA AAATCATCAA GGAGAGGGAG CTGGAGCTGC AGAAGGAACA GCGGCGGCAG 
GAGGAGAACG ACAAGCTGCG CCAGGAGTTT GCCCAGCACG CCAACGCCTT CCACCAGTGG 
ATCCAAGAGA CCAGGACATA CCTCCTCGAT GGGTCCTGTA TGGTGGAAGA GTCGGGGACC 
CTCGAATCCC AGCTTGAAGC TACCAAACGC AAGCACCAGG AAATCCGAGC CATGAGAAGT 
CAGCTCAAAA AGATCGAGGA CCTGGGGGCC GCCATGGAGG AGGCCCTCAT CCTGGACAAC 
AAGTACACGG AGCACAGCAC CGTGGGCCTC GCCCAGCAGT GGGACCAGCT GGACCAGCTG 
GGCATGCGCA TGCAGCACAA CCTGGAGCAG CAGATCCAGG CCAGGAACAC AACAGGTGTG 
ACTGAGGAGG CCCTCAAAGA ATTCAGCATG ATGTTTAAAC ACTTTGACAA GGACAAGTCT 
GGCAGGCTGA ACCATCAGGA GTTCAAATCT TGCCTGCGCT CCCTGGGCTA TGACCTGCCC 
ATGGTGGAGG AAGGGGAACC TGACCCTGAG TTCGAGGCAA TCCTGGACAC GGTGGATCCG 
AACAGAGATG GCCATGTCTC CCTGCAAGAA TACATGGCTT TCATGATCAG CCGCGAAACT 
GAGAACGTCA AGTCCAGCGA GGAGATTGAG AGCGCCTTCC GGGCCCTCAG CTCAGAGGGA 
AAGCCTTACG TGACCAAGGA GGAGCTCTAC CAGAACCTGA CCCGGGAACA AGCCGACTAC 
TGCGTCTCCC ACATGAAGCC CTACGTGGAC GGCAAGGGCC GCGAGCTCCC CACCGCGTTC 
GACTACGTGG AGTTCACCCG CTCGCTTTTC GTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009455701.2
CDS	137..7555
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVNHYAKDEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRITKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKQKLDILDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQAFADQLIAAGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINGRFQKIKSMAASRRAKLNESHRLHQFFRDMDDEESWIKEKKLLVGSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHASLMKRWSQLLANSAARKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_009457426.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009457426.2

ORF Insert Sequence:

ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTAG ACCGATACCA CCGCTTCAAG GAACTCTCAA CCCTTAGGCG TCAGAAGCTG 
GAAGATTCCT ATCGATTCCA GTTCTTTCAA AGAGATGCTG AAGAGCTGGA GAAATGGATA 
CAGGAAAAAC TTCAGATTGC ATCTGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGA 
AAGCTTCAGA AGCATCAAGC ATTTGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT 
AAGCTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCATC TGAAACCATA 
CGGACCCGTT TGATGGAGCT GCACCGCCAG TGGGAATTAC TTTTGGAGAA GATGCGAGAA 
AAAGGAATCA AACTGCTGCA GGCCCAGAAG TTGGTGCAGT ACTTACGAGA ATGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCA ATTGTTACTT CTGAAGAGCT GGGCCAGGAT 
CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCT 
CATGAAGAAA GAGTTAATGA AGTGAACCAA TTTGCTGCCA AACTCATACA GGAGCAGCAC 
CCTGAGGAGG AACTGATCAA GACTAAGCAG GATGAAGTCA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CTCTGCAGAG GCAGGGGAAG CTCTTTGGGG CAGCAGAAGT TCAGCGCTTT 
AACAGGGATG TGGATGAGAC TATCAGTTGG ATTAAGGAAA AGGAGCAGTT AATGGCCTCT 
GATGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TTCGGAAGCA CGAGGGTCTG 
GAGAGAGATC TTGCTGCTCT AGAAGACAAG GTCAAAGCCC TGTGTGCTGA GGCTGACCGC 
CTGCAACAGT CCCACCCTCT GAGTGCAACA CAGATTCAAG TGAAGCGAGA GGAACTGATT 
ACAAACTGGG AGCAGATCCG CACCTTGGCG GCAGAGAGAC ATGCACGGCT CAATGATTCA 
TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTCA CCAGCTGGGT GACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAGCTTGCC AATGATGTGG CTGGGGCTGA AGCCCTGCTA 
GATAGACACC AAGAACACAA GGGTGAAATT GATGCCCATG AAGACAGCTT CAAATCTGCA 
GATGAATCTG GACAGGCACT GCTTGCTGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCG TCCTTTCCGA GGAGAGAGCG GCGCTGCTGG AGCTGTGGGA GCTGCGCAGG 
CAGCAGTACG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GGGACACTGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAATGAAG ACTTGGGAGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTTAA GAAGCACGAA GACTTTGAGA AATCCCTTAG TGCCCAGGAG 
GAAAAGATTA CAGCATTAGA TGAATTTGCA ACCAAGCTAA TTCAGAACAA CCACTATGCA 
ATGGAAGATG TGGCCACTCG CCGAGATGCT CTGTTGAGCC GCCGCAATGC CCTTCACGAG 
AGAGCCATGC GTCGCCGGGC CCAGCTAGCC GATTCTTTCC ATCTGCAGCA GTTTTTCCGT 
GATTCTGATG AGCTCAAGAG TTGGGTCAAT GAGAAGATGA AAACTGCCAC AGATGAAGCT 
TATAAAGATC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG 
CTCTCAGCAA ACCAGAGCCG AATTGATGCC TTGGAGAAAG CTGGCCAAAA GCTGATTGAT 
GTCAACCACT ATGCCAAGGA TGAAGTGGCA GCTCGTATGA ATGAGGTGAT CAGTTTGTGG 
AAGAAACTGC TAGAGGCCAC TGAACTGAAA GGAATAAAGC TTCGTGAAGC CAACCAGCAA 
CAGCAATTTA ATCGCAATGT TGAGGATATT GAATTGTGGC TATATGAAGT AGAAGGTCAC 
TTGGCTTCGG ATGATTACGG CAAAGATCTT ACCAATGTGC AGAACCTCCA GAAGAAACAT 
GCCCTGCTAG AGGCAGATGT GGCTGCTCAT CAGGACCGAA TTGATGGCAT TACCATTCAG 
GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCAGAAA ACATCAAGAA GAAACAGGAA 
GCCCTCGTGG CTCGCTATGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCTC TGCGGTTGCA GCAGCTCTTC CGGGATGTTG AGGATGAGGA GACGTGGATT 
CGAGAGAAGG AGCCCATTGC CGCATCTACC AACAGAGGTA AGGATTTAAT TGGGGTCCAG 
AATCTGCTAA AGAAACATCA AGCCTTACAA GCAGAAATTG CTGGACATGA ACCACGCATC 
AAAGCAGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT 
GTGAAGGCCA AGCTTCACGA GCTGAACCAA AAGTGGGAGG CACTGAAAGC CAAAGCTTCC 
CAGCGTCGGC AGGACCTGGA GGACTCTCTG CAGGCCCAGC AGTACTTTGC TGATGCTAAC 
GAGGCTGAAT CCTGGATGCG GGAGAAGGAA CCCATTGTGG GCAGCACTGA CTATGGCAAG 
GACGAAGACT CTGCTGAGGC TCTACTGAAG AAACACGAAG CTTTGATGTC AGATCTCAGT 
GCCTACGGCA GCAGCATCCA GGCTTTGCGA GAACAAGCAC AGTCCTGCCG GCAACAAGTG 
GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAG GGAGATATCC TTACCTTACT CAACAGCACC 
AACAAGGATT GGTGGAAAGT GGAAGTGAAC GATCGTCAGG GTTTTGTGCC AGCTGCTTAC 
GTGAAGAAAT TGGACCCCGC CCAGTCAGCC TCCCGGGAGA ATCTCCTGGA GGAGCAAGGC 
AGCATAGCAC TGCGGCAGGA GCAGATTGAC AATCAGACAC GCATAACTAA GGAGGCCGGC 
AGTGTATCTC TGCGTATGAA GCAGGTGGAA GAACTATATC ATTCTCTGCT GGAACTGGGT 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TTATGTTGTT CCGTGAAGCG 
AATGAACTAC AGCAATGGAT CAATGAGAAG GAAGCCGCTC TGACAAGTGA GGAGGTCGGA 
GCAGACTTGG AGCAGGTTGA GGTGCTCCAG AAGAAGTTTG ATGACTTCCA GAAGGACCTG 
AAGGCCAATG AGTCACGGTT GAAGGACATT AACAAGGTAG CTGAAGACCT GGAGTCTGAA 
GGTCTCATGG CAGAGGAGGT GCAGGCTGTG CAACAACAGG AAGTGTATGG CATGATGCCC 
AGGGATGAAA CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGTCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAATGAGC GCTGGCGGTC CCTACAGCAG 
CTGGCCGAGG AACGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA 
GATGCTGATG AAACCAAAGA ATGGATTGAA GAGAAGAATC AGGCTCTAAA CACAGACAAT 
TATGGACATG ATCTTGCCAG TGTCCAGGCC CTGCAACGCA AGCATGAGGG CTTCGAGAGG 
GACCTTGCGG CTCTCGGTGA CAAGGTAAAC TCCCTTGGTG AAACAGCAGA GCGCCTGATC 
CAATCCCATC CCGAGTCAGC AGAAGACCTG CAGGAAAAGT GCACAGAGTT AAACCAGGCC 
TGGAGCAGCC TGGGGAAACG TGCAGATCAG CGCAAGGCAA AGTTGGGTGA CTCCCACGAC 
CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGGGGG 
TTGGTGTCCT CAGATGAGCT AGCCAAGGAT GTCACCGGAG CTGAGGCGTT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG 
TTTGGACAGC AGCTGTTGGC TCACGGACAC TATGCCAGCC CTGAGATCAA GCAGAAACTT 
GATATTCTTG ACCAGGAGCG TGCAGACCTG GAGAAGGCCT GGGTTCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAACT GCAGCTGTTC CATCGGGACT GTGAGCAAGC TGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CTTGAATACC GAAGACAAAG GAGACTCACT GGACAGCGTA 
GAGGCTCTGA TCAAAAAACA TGAAGACTTT GACAAAGCGA TTAACGTCCA GGAAGAGAAG 
ATTGCTGCTC TGCAGGCCTT TGCCGACCAG CTCATCGCTG CCGGCCATTA TGCCAAGGGA 
GACATTTCTA GCCGGCGCAA TGAGGTCTTG GACAGGTGGC GACGTCTGAA AGCCCAAATG 
ATTGAGAAAA GGTCAAAGCT AGGAGAATCT CAAACCCTCC AACAGTTCAG CCGGGATGTG 
GATGAGATTG AGGCTTGGAT CAGTGAAAAA TTGCAAACGG CAAGTGATGA GTCATACAAG 
GATCCCACCA ACATCCAGAG CAAGCACCAG AAGCACCAGG CTTTTGAAGC AGAGCTGCAT 
GCCAACGCTG ACCGGATCCG TGGGGTTATC GACATGGGCA ACTCCCTCAT TGAACGTGGA 
GCCTGTGCCG GCAGTGAGGA TGCTGTCAAG GCCCGCCTGG CTGCCTTAGC TGACCAGTGG 
CAATTCTTGG TGCAAAAGTC AGCGGAAAAG AGCCAGAAAC TGAAAGAAGC CAACAAGCAG 
CAGAACTTCA ACACAGGGAT CAAGGACTTT GACTTCTGGC TGTCTGAGGT GGAGGCCCTG 
CTGGCATCCG AAGATTATGG CAAAGACCTG GCTTCTGTGA ACAACCTGCT GAAAAAGCAT 
CAACTGCTGG AAGCAGATAT ATCTGCCCAT GAGGATCGCC TGAAGGACCT GAACAGCCAG 
GCAGACAGCC TGATGACCAG CAGTGCCTTC GACACCTCCC AAGTAAAGGA CAAGAGGGAC 
ACCATCAACG GGCGCTTCCA GAAGATCAAG AGCATGGCGG CCTCCCGGCG AGCCAAGCTG 
AATGAATCCC ATCGCCTGCA CCAGTTCTTC CGGGACATGG ATGACGAGGA GTCCTGGATC 
AAGGAGAAGA AGCTGCTGGT GGGCTCGGAG GACTACGGCC GGGACCTAAC CGGCGTGCAG 
AACCTGAGGA AGAAGCACAA GCGCCTGGAA GCAGAACTGG CTGCGCATGA GCCGGCTATT 
CAGGGTGTCC TGGACACTGG CAAGAAGCTG TCCGATGATA ACACCATCGG GAAAGAGGAG 
ATCCAGCAGC GGCTGGCGCA GTTTGTGGAG CACTGGAAAG AGCTGAAGCA GCTGGCAGCT 
GCCCGGGGTC AGCGGCTGGA AGAGTCCTTG GAATATCAGC AGTTTGTAGC CAATGTGGAA 
GAGGAGGAAG CCTGGATCAA TGAGAAAATG ACCCTGGTGG CCAGCGAAGA TTATGGCGAC 
ACTCTTGCTG CCATCCAGGG CTTACTGAAG AAACATGAAG CTTTTGAGAC AGACTTCACC 
GTCCACAAGG ATCGCGTGAA TGACGTCTGC ACCAATGGAC AAGACCTCAT TAAGAAGAAC 
AATCACCATG AGGAGAACAT CTCTTCAAAG ATGAAGGGCC TGAACGGGAA AGTGTCAGAC 
CTGGAGAAAG CTGCAGCCCA GAGAAAGGCG AAGCTGGATG AGAACTCGGC CTTCCTTCAG 
TTCAACTGGA AGGCGGACGT GGTGGAGTCC TGGATCGGTG AAAAGGAGAA CAGCTTGAAG 
ACAGATGATT ATGGCCGAGA CCTGTCTTCT GTGCAGACGC TCCTCACCAA ACAGGAAACT 
TTTGACGCTG GGCTGCAGGC CTTCCAGCAG GAAGGCATTG CCAACATCAC TGCCCTCAAA 
GATCAGCTTC TCGCCGCCAA ACACGTTCAG TCCAAGGCCA TCGAGGCCCG GCACGCCTCC 
CTCATGAAGA GGTGGAGCCA GCTTCTGGCC AACTCAGCCG CCCGCAAGAA GAAGCTTCTG 
GAGGCGCAGA GTCACTTCCG CAAGGTGGAG GACCTCTTCC TGACCTTTGC CAAAAAGGCT 
TCTGCCTTCA ACAGCTGGTT TGAAAATGCA GAGGAGGACT TAACAGACCC CGTGCGCTGC 
AACTCCTTGG AAGAAATCAA AGCTTTGCGC GAGGCCCACG ACGCCTTCCG CTCCTCCCTC 
AGCTCTGCCC AGGCTGACTT CAACCAGCTG GCCGAGCTGG ACCGCCAGAT CAAGAGCTTC 
CGCGTAGCCT CCAACCCCTA CACCTGGTTT ACCATGGAGG CCCTGGAGGA GACCTGGAGG 
AACCTACAGA AAATCATCAA GGAGAGGGAG CTGGAGCTGC AGAAGGAACA GCGGCGGCAG 
GAGGAGAACG ACAAGCTGCG CCAGGAGTTT GCCCAGCACG CCAACGCCTT CCACCAGTGG 
ATCCAAGAGA CCAGGACATA CCTCCTCGAT GGGTCCTGTA TGGTGGAAGA GTCGGGGACC 
CTCGAATCCC AGCTTGAAGC TACCAAACGC AAGCACCAGG AAATCCGAGC CATGAGAAGT 
CAGCTCAAAA AGATCGAGGA CCTGGGGGCC GCCATGGAGG AGGCCCTCAT CCTGGACAAC 
AAGTACACGG AGCACAGCAC CGTGGGCCTC GCCCAGCAGT GGGACCAGCT GGACCAGCTG 
GGCATGCGCA TGCAGCACAA CCTGGAGCAG CAGATCCAGG CCAGGAACAC AACAGGTGTG 
ACTGAGGAGG CCCTCAAAGA ATTCAGCATG ATGTTTAAAC ACTTTGACAA GGACAAGTCT 
GGCAGGCTGA ACCATCAGGA GTTCAAATCT TGCCTGCGCT CCCTGGGCTA TGACCTGCCC 
ATGGTGGAGG AAGGGGAACC TGACCCTGAG TTCGAGGCAA TCCTGGACAC GGTGGATCCG 
AACAGAGATG GCCATGTCTC CCTGCAAGAA TACATGGCTT TCATGATCAG CCGCGAAACT 
GAGAACGTCA AGTCCAGCGA GGAGATTGAG AGCGCCTTCC GGGCCCTCAG CTCAGAGGGA 
AAGCCTTACG TGACCAAGGA GGAGCTCTAC CAGAACCTGA CCCGGGAACA AGCCGACTAC 
TGCGTCTCCC ACATGAAGCC CTACGTGGAC GGCAAGGGCC GCGAGCTCCC CACCGCGTTC 
GACTACGTGG AGTTCACCCG CTCGCTTTTC GTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 cDNA ORF clone, Pan troglodytes(chimpanzee)

Summary

Reference Sequences (RefSeq)

Pathways from BioSystems

Homologies

Gene Ontology

Reference Sequences (Refseq)

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