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Otulin cDNA ORF clone, Rattus norvegicus(Norway rat)

Summary

Gene Symbol Otulin
Entrez Gene ID 100362554
Full Name OTU deubiquitinase with linear linkage specificity
Synonyms Fam105b
General protein information
Preferred Names

OTU deubiquitinase with linear linkage specificity

Names

ubiquitin thioesterase otulin
family with sequence similarity 105, member B
Gene Type protein-coding
Organism Rattus norvegicus(Norway rat)
Genome

2

2q22

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_006232088.3 XP_006232150.1 ubiquitin thioesterase otulin isoform X1
NM_001302889.1 NP_001289818.1 ubiquitin thioesterase otulin

Pathways from BioSystems


Homologies


FAM105B XP_001087122.2
Rattus norvegicus (Norway rat) Fam105b XP_006232150.1
Gallus gallus (chicken) FAM105B XP_004935161.1
Homo sapiens (human) FAM105B NP_612357.4
Danio rerio (zebrafish) fam105bb NP_001159487.1
Pan troglodytes (chimpanzee) FAM105B XP_517640.2
Canis lupus familiaris (dog) FAM105B XP_848253.2
Bos taurus (cattle) FAM105B NP_001093798.1
Mus musculus (house mouse) Fam105b NP_001013814.2
Danio rerio (zebrafish) LOC100001665 XP_001341618.4
Xenopus tropicalis (tropical clawed frog) fam105b XP_004915385.1

Gene Ontology


Bibliography

Related articles in PubMed

Biallelic hypomorphic mutations in a linear deubiquitinase define otulipenia, an early-onset autoinflammatory disease.
Zhou Q, Yu X, Demirkaya E, Deuitch N, Stone D, Tsai WL, Kuehn HS, Wang H, Yang D, Park YH, Ombrello AK, Blake M, Romeo T, Remmers EF, Chae JJ, Mullikin JC, G?zel F, Milner JD, Boehm M, Rosenzweig SD, Gadina M, Welch SB, ?zen S, Topaloglu R, Abinun M, Kastner DL, Aksentijevich I
Proceedings of the National Academy of Sciences of the United States of America113(36)10127-32(2016 09)

The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity.
Damgaard RB, Walker JA, Marco-Casanova P, Morgan NV, Titheradge HL, Elliott PR, McHale D, Maher ER, McKenzie ANJ, Komander D
Cell166(5)1215-1230.e20(2016 Aug)

CYLD Limits Lys63- and Met1-Linked Ubiquitin at Receptor Complexes to Regulate Innate Immune Signaling.
Hrdinka M, Fiil BK, Zucca M, Leske D, Bagola K, Yabal M, Elliott PR, Damgaard RB, Komander D, Jost PJ, Gyrd-Hansen M
Cell reports14(12)2846-58(2016 Mar)

Binding of OTULIN to the PUB domain of HOIP controls NF-?B signaling.
Schaeffer V, Akutsu M, Olma MH, Gomes LC, Kawasaki M, Dikic I
Molecular cell54(3)349-61(2014 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Otulin gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Otulin cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa15130 NM_001302889.1
Latest version!		 Rattus norvegicus OTU deubiquitinase with linear linkage specificity (Otulin), mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $223.30
$319.00
ORa15131 XM_006232088.3
Latest version!		 Rattus norvegicus OTU deubiquitinase with linear linkage specificity (Otulin), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $223.30
$319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID ORa15130
Clone ID Related Accession (Same CDS sequence) NM_001302889.1
Accession Version NM_001302889.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1062bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-04-18
Organism Rattus norvegicus(Norway rat)
Product ubiquitin thioesterase otulin
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07008940.1, BC158613.1, FM089678.1, BC158827.1 and FM122033.1. On or before Nov 27, 2014 this sequence version replaced XM_002725921.4, XM_002729016.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC158613.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGAGTCGGG GAATCATGCC CCAACCCGGA GCGTGGCCGG GAGCGGGCTG CGCGGAGACG 
CCGGCGCGCG AGGCAGGGGC TGCGGCGCGG GACGGCGGGA AGGTGACGGT CGGCGCGCAG 
CCCCGGGCCG CGACGCGATG CCCGGCCGAG CACGAGGAGG ACATGTACCG TGCTGCAGAT 
GAAATAGAAA AGGAGAAAGA ATTGCTAATA CATGAAAGAG GGATATCAGC AGAACCCAGG 
TTAAGTGTGG CTCCTGAAAT GGATATTATG GACTACTGCA AAAAGGAATG GAGAGGAAAT 
ACCGAGAAAG CCATGTGCAT GAGAAAGGGC TATGAGGAAG TTTCTCAGAA GTTCACTTCC 
ATAAGGCGAG TCCGTGGCGA TAATTACTGT GCACTGCGGG CCACACTGTT CCAGGCTATG 
AGCCAGCTGG CTGAGCTGCC CCCCTGGCTG CAGGACCTGG AGCTCATACT GTTACCGGAA 
AAGCTGATAA ACAAATATAC CTGGATCAAG CAGTGGAAAC TTGGACTGAA ATTTGATGGG 
AAGAGTGAGG ACCTGGTTGA AAGAATCAAG GAATCCCTTG CCCTGCTAAG GAAGAAGTGG 
GTCAGCCTGG CTGCAATGGA AGCCGCTGAA GCAAGGCAGA CAGCCTGTGA CGAGCTGTTC 
ACAAACGAGG AGGAGGAGTA CAGCCTCTAC GAAGCTATGA AGTTCCTGAT GTTAGCCAGA 
GCCATCCAAC TGTATGATGA CAAGGAAAAG GGAAAGGAAG TTCCTTTTTT CTCTGTGCTC 
TTGTTTGCCC GAGACACATC AAATGACCCT GAACAGCTCC TGAGGAACCA CCTGAACCAG 
GTGGGACACA CTGGCGGCCT TGAACAGGTT GAAATGTTCC TTCTTGCCTA TGCTGTTCGC 
CACACCATCC AGGTGTACCG GCTGTCCAAG TATAACACTG AGGAATTCAT CACAGTCTAT 
CCCACTGATC CCCCCAAGGA CTGGCCAATG GTGACCCTCA TAGCTGAGGA CGATCGGCAC 
TATAACATCC CTGTCAGAGT GTGTGAGGAG ACAAGTCTGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001289818.1
CDS73..1134
Translation

Target ORF information:

RefSeq Version NM_001302889.1
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus OTU deubiquitinase with linear linkage specificity (Otulin), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001302889.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGAGTCGGG GAATCATGCC CCAACCCGGA GCGTGGCCGG GAGCGGGCTG CGCGGAGACG 
CCGGCGCGCG AGGCAGGGGC TGCGGCGCGG GACGGCGGGA AGGTGACGGT CGGCGCGCAG 
CCCCGGGCCG CGACGCGATG CCCGGCCGAG CACGAGGAGG ACATGTACCG TGCTGCAGAT 
GAAATAGAAA AGGAGAAAGA ATTGCTAATA CATGAAAGAG GGATATCAGC AGAACCCAGG 
TTAAGTGTGG CTCCTGAAAT GGATATTATG GACTACTGCA AAAAGGAATG GAGAGGAAAT 
ACCGAGAAAG CCATGTGCAT GAGAAAGGGC TATGAGGAAG TTTCTCAGAA GTTCACTTCC 
ATAAGGCGAG TCCGTGGCGA TAATTACTGT GCACTGCGGG CCACACTGTT CCAGGCTATG 
AGCCAGCTGG CTGAGCTGCC CCCCTGGCTG CAGGACCTGG AGCTCATACT GTTACCGGAA 
AAGCTGATAA ACAAATATAC CTGGATCAAG CAGTGGAAAC TTGGACTGAA ATTTGATGGG 
AAGAGTGAGG ACCTGGTTGA AAGAATCAAG GAATCCCTTG CCCTGCTAAG GAAGAAGTGG 
GTCAGCCTGG CTGCAATGGA AGCCGCTGAA GCAAGGCAGA CAGCCTGTGA CGAGCTGTTC 
ACAAACGAGG AGGAGGAGTA CAGCCTCTAC GAAGCTATGA AGTTCCTGAT GTTAGCCAGA 
GCCATCCAAC TGTATGATGA CAAGGAAAAG GGAAAGGAAG TTCCTTTTTT CTCTGTGCTC 
TTGTTTGCCC GAGACACATC AAATGACCCT GAACAGCTCC TGAGGAACCA CCTGAACCAG 
GTGGGACACA CTGGCGGCCT TGAACAGGTT GAAATGTTCC TTCTTGCCTA TGCTGTTCGC 
CACACCATCC AGGTGTACCG GCTGTCCAAG TATAACACTG AGGAATTCAT CACAGTCTAT 
CCCACTGATC CCCCCAAGGA CTGGCCAATG GTGACCCTCA TAGCTGAGGA CGATCGGCAC 
TATAACATCC CTGTCAGAGT GTGTGAGGAG ACAAGTCTGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa15131
Clone ID Related Accession (Same CDS sequence) XM_006232088.3
Accession Version XM_006232088.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1059bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product ubiquitin thioesterase otulin isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006232088.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGAGTCGGG GAATCATGCC CCAACCCGGA GCGTGGCCGG GAGCGGGCTG CGCGGAGACG 
CCGGCGCGCG AGGCAGGGGC TGCGGCGCGG GACGGCGGGA AGGTGACGGT CGGCGCGCAG 
CCCCGGGCCG CGACGCGATG CCCGGCCGAG CACGAGGAGG ACATGTACCG TGCTGCAGAT 
GAAATAGAAA AGGAGAAAGA ATTGCTAATA CATGAAAGAG GGATATCAGA ACCCAGGTTA 
AGTGTGGCTC CTGAAATGGA TATTATGGAC TACTGCAAAA AGGAATGGAG AGGAAATACC 
GAGAAAGCCA TGTGCATGAG AAAGGGCTAT GAGGAAGTTT CTCAGAAGTT CACTTCCATA 
AGGCGAGTCC GTGGCGATAA TTACTGTGCA CTGCGGGCCA CACTGTTCCA GGCTATGAGC 
CAGCTGGCTG AGCTGCCCCC CTGGCTGCAG GACCTGGAGC TCATACTGTT ACCGGAAAAG 
CTGATAAACA AATATACCTG GATCAAGCAG TGGAAACTTG GACTGAAATT TGATGGGAAG 
AGTGAGGACC TGGTTGAAAG AATCAAGGAA TCCCTTGCCC TGCTAAGGAA GAAGTGGGTC 
AGCCTGGCTG CAATGGAAGC CGCTGAAGCA AGGCAGACAG CCTGTGACGA GCTGTTCACA 
AACGAGGAGG AGGAGTACAG CCTCTACGAA GCTATGAAGT TCCTGATGTT AGCCAGAGCC 
ATCCAACTGT ATGATGACAA GGAAAAGGGA AAGGAAGTTC CTTTTTTCTC TGTGCTCTTG 
TTTGCCCGAG ACACATCAAA TGACCCTGAA CAGCTCCTGA GGAACCACCT GAACCAGGTG 
GGACACACTG GCGGCCTTGA ACAGGTTGAA ATGTTCCTTC TTGCCTATGC TGTTCGCCAC 
ACCATCCAGG TGTACCGGCT GTCCAAGTAT AACACTGAGG AATTCATCAC AGTCTATCCC 
ACTGATCCCC CCAAGGACTG GCCAATGGTG ACCCTCATAG CTGAGGACGA TCGGCACTAT 
AACATCCCTG TCAGAGTGTG TGAGGAGACA AGTCTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006232150.1
CDS91..1149
Translation

Target ORF information:

RefSeq Version XM_006232088.3
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus OTU deubiquitinase with linear linkage specificity (Otulin), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006232088.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGAGTCGGG GAATCATGCC CCAACCCGGA GCGTGGCCGG GAGCGGGCTG CGCGGAGACG 
CCGGCGCGCG AGGCAGGGGC TGCGGCGCGG GACGGCGGGA AGGTGACGGT CGGCGCGCAG 
CCCCGGGCCG CGACGCGATG CCCGGCCGAG CACGAGGAGG ACATGTACCG TGCTGCAGAT 
GAAATAGAAA AGGAGAAAGA ATTGCTAATA CATGAAAGAG GGATATCAGA ACCCAGGTTA 
AGTGTGGCTC CTGAAATGGA TATTATGGAC TACTGCAAAA AGGAATGGAG AGGAAATACC 
GAGAAAGCCA TGTGCATGAG AAAGGGCTAT GAGGAAGTTT CTCAGAAGTT CACTTCCATA 
AGGCGAGTCC GTGGCGATAA TTACTGTGCA CTGCGGGCCA CACTGTTCCA GGCTATGAGC 
CAGCTGGCTG AGCTGCCCCC CTGGCTGCAG GACCTGGAGC TCATACTGTT ACCGGAAAAG 
CTGATAAACA AATATACCTG GATCAAGCAG TGGAAACTTG GACTGAAATT TGATGGGAAG 
AGTGAGGACC TGGTTGAAAG AATCAAGGAA TCCCTTGCCC TGCTAAGGAA GAAGTGGGTC 
AGCCTGGCTG CAATGGAAGC CGCTGAAGCA AGGCAGACAG CCTGTGACGA GCTGTTCACA 
AACGAGGAGG AGGAGTACAG CCTCTACGAA GCTATGAAGT TCCTGATGTT AGCCAGAGCC 
ATCCAACTGT ATGATGACAA GGAAAAGGGA AAGGAAGTTC CTTTTTTCTC TGTGCTCTTG 
TTTGCCCGAG ACACATCAAA TGACCCTGAA CAGCTCCTGA GGAACCACCT GAACCAGGTG 
GGACACACTG GCGGCCTTGA ACAGGTTGAA ATGTTCCTTC TTGCCTATGC TGTTCGCCAC 
ACCATCCAGG TGTACCGGCT GTCCAAGTAT AACACTGAGG AATTCATCAC AGTCTATCCC 
ACTGATCCCC CCAAGGACTG GCCAATGGTG ACCCTCATAG CTGAGGACGA TCGGCACTAT 
AACATCCCTG TCAGAGTGTG TGAGGAGACA AGTCTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Biallelic hypomorphic mutations in a linear deubiquitinase define otulipenia, an early-onset autoinflammatory disease.
Proceedings of the National Academy of Sciences of the United States of America113(36)10127-32(2016 09)
Zhou Q,Yu X,Demirkaya E,Deuitch N,Stone D,Tsai WL,Kuehn HS,Wang H,Yang D,Park YH,Ombrello AK,Blake M,Romeo T,Remmers EF,Chae JJ,Mullikin JC,G?zel F,Milner JD,Boehm M,Rosenzweig SD,Gadina M,Welch SB,?zen S,Topaloglu R,Abinun M,Kastner DL,Aksentijevich I


book

The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity.
Cell166(5)1215-1230.e20(2016 Aug)
Damgaard RB,Walker JA,Marco-Casanova P,Morgan NV,Titheradge HL,Elliott PR,McHale D,Maher ER,McKenzie ANJ,Komander D


book

CYLD Limits Lys63- and Met1-Linked Ubiquitin at Receptor Complexes to Regulate Innate Immune Signaling.
Cell reports14(12)2846-58(2016 Mar)
Hrdinka M,Fiil BK,Zucca M,Leske D,Bagola K,Yabal M,Elliott PR,Damgaard RB,Komander D,Jost PJ,Gyrd-Hansen M


book

Binding of OTULIN to the PUB domain of HOIP controls NF-?B signaling.
Molecular cell54(3)349-61(2014 May)
Schaeffer V,Akutsu M,Olma MH,Gomes LC,Kawasaki M,Dikic I