ORF cDNA Clones and Custom Clones » Species Summary » Rattus norvegicus » Cacna1a cDNA ORF clone

Cacna1a cDNA ORF clone, Rattus norvegicus(Norway rat)

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Summary

Gene Symbol	Cacna1a
Entrez Gene ID	25398
Full Name	calcium voltage-gated channel subunit alpha1 A
Synonyms	BccA1,Cav2.1,rbA-1
General protein information	Preferred Names calcium voltage-gated channel subunit alpha1 A Names voltage-dependent P/Q-type calcium channel subunit alpha-1A BI RBA-I brain calcium channel 1 brain calcium channel I brain class A calcium channel alpha 1A calcium channel, L type, alpha-1 polypeptide calcium channel, voltage-dependent, P/Q type, alpha 1A subunit calcium channel, voltage-dependent, alpha 1A subunit voltage-gated calcium channel subunit alpha Cav2.1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 19 Map Location: 19q11
Summary	voltage-gated calcium channel that may be involved in secretion coupling in pancreatic beta cells [RGD, Feb 2006].

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008772348.2	XP_008770570.2	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X2
XM_008772350.2	XP_008770572.2	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X4
XM_017601178.1	XP_017456667.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X8
XM_017601180.1	XP_017456669.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X11
XM_017601182.1	XP_017456671.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X13
XM_008772360.2	XP_008770582.2	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X16
XM_017601177.1	XP_017456666.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X1
XM_008772359.2	XP_008770581.2	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X15
XM_008772349.2	XP_008770571.2	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X3
XM_017601179.1	XP_017456668.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X10
XM_008772356.2	XP_008770578.2	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X12
NM_012918.3	NP_037050.2	voltage-dependent P/Q-type calcium channel subunit alpha-1A

Homologies

Homo sapiens (human)	CACNA1A	NP_001120694.1
Pan troglodytes (chimpanzee)	CACNA1A	XP_003316202.1
Xenopus tropicalis (tropical clawed frog)	cacna1a	XP_004919196.1
	CACNA1A	XP_002801141.1
Danio rerio (zebrafish)	cacna1ab	XP_698014.6
Canis lupus familiaris (dog)	CACNA1A	XP_005632867.1
Bos taurus (cattle)	CACNA1A	NP_001068597.1
Mus musculus (house mouse)	Cacna1a	NP_031604.3
Rattus norvegicus (Norway rat)	Cacna1a	NP_037050.2

Bibliography

Related articles in PubMed

Alcohol withdrawal upregulates mRNA encoding for Ca
Newton J, Suman S, Akinfiresoye LR, Datta K, Lovinger DM, N'Gouemo P
Alcohol (Fayetteville, N.Y.)6621-26(2018 Feb)

A CaV2.1 N-terminal fragment relieves the dominant-negative inhibition by an Episodic ataxia 2 mutant.
Dahimene S, Page KM, Nieto-Rostro M, Pratt WS, D'Arco M, Dolphin AC
Neurobiology of disease93243-56(2016 Sep)

A Single Amino Acid Deletion (?F1502) in the S6 Segment of CaV2.1 Domain III Associated with Congenital Ataxia Increases Channel Activity and Promotes Ca2+ Influx.
Bahamonde MI, Serra SA, Drechsel O, Rahman R, Marc?-Grau A, Prieto M, Ossowski S, Macaya A, Fern?ndez-Fern?ndez JM
PloS one10(12)e0146035(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the consensus motifs of S-nitrosylation were much more abundant in Cav2.2 than in Cav1.2 and Cav2.1.
Title: Molecular Basis of Regulating High Voltage-Activated Calcium Channels by S-Nitrosylation.

Thus, GHSR1a differentially inhibits CaV2 channels by Gi/o or Gq protein pathways depending on its mode of activation.
Title: Constitutive and ghrelin-dependent GHSR1a activation impairs CaV2.1 and CaV2.2 currents in hypothalamic neurons.

data suggest that the different roles that Ca(V)2.1 and Ca(V)2.2 play in MNC secretion may be a result of the different levels of expression of Ca(V)2.1 in VP and OT MNCs
Title: Expression of CaV 2.2 and splice variants of CaV 2.1 in oxytocin- and vasopressin-releasing supraoptic neurones.

By transducing presynaptic action potential firings into unique Ca(2+) signals and vesicle release profiles, Cav2.1 channels contribute to the encoding and processing of neural information
Title: Cav2.1 channels control multivesicular release by relying on their distance from exocytotic Ca2+ sensors at rat cerebellar granule cells.

the contribution of Ca(V)2.1 to complex cognitive functions
Title: Ablation of Ca(V)2.1 voltage-gated Ca?? channels in mouse forebrain generates multiple cognitive impairments.

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The following Cacna1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cacna1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa39897	NM_012918.3 Latest version!	Rattus norvegicus calcium voltage-gated channel subunit alpha1 A (Cacna1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$2092.30 $2989.00
ORa59364	XM_017601177.1 Latest version!	Rattus norvegicus calcium voltage-gated channel subunit alpha1 A (Cacna1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
ORa59365	XM_008772348.2 Latest version!	Rattus norvegicus calcium voltage-gated channel subunit alpha1 A (Cacna1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
ORa59366	XM_008772349.2 Latest version!	Rattus norvegicus calcium voltage-gated channel subunit alpha1 A (Cacna1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
ORa59367	XM_008772350.2 Latest version!	Rattus norvegicus calcium voltage-gated channel subunit alpha1 A (Cacna1a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
ORa59371	XM_017601178.1 Latest version!	Rattus norvegicus calcium voltage-gated channel subunit alpha1 A (Cacna1a), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
ORa59373	XM_017601179.1 Latest version!	Rattus norvegicus calcium voltage-gated channel subunit alpha1 A (Cacna1a), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
ORa59374	XM_017601180.1 Latest version!	Rattus norvegicus calcium voltage-gated channel subunit alpha1 A (Cacna1a), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
ORa59375	XM_008772356.2 Latest version!	Rattus norvegicus calcium voltage-gated channel subunit alpha1 A (Cacna1a), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
ORa59376	XM_017601182.1 Latest version!	Rattus norvegicus calcium voltage-gated channel subunit alpha1 A (Cacna1a), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
ORa59378	XM_008772359.2 Latest version!	Rattus norvegicus calcium voltage-gated channel subunit alpha1 A (Cacna1a), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
ORa59379	XM_008772360.2 Latest version!	Rattus norvegicus calcium voltage-gated channel subunit alpha1 A (Cacna1a), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cacna1a ( NM_012918.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037050.2 Rattus norvegicus calcium voltage-gated channel subunit alpha1 A (Cacna1a), mRNA.

Price & Availability↑

CloneID	ORa39897
Clone ID Related Accession (Same CDS sequence)	NM_012918.3
Accession Version	NM_012918.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from M64373.1, AF051526.1, CB582288.1 and AC120246.5. On Jul 7, 2010 this sequence version replaced NM_012918.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCCCGCT TTGGAGACGA GATGCCGGGC CGCTACGGCG CAGGCGGAGG AGGCTCAGGG 
CCGGCCGCCG GGGTGGTCGT GGGCGCCGCG GGCGGCCGAG GAGCCGGGGG CAGCCGGCAG 
GGCGGGCAGC CCGGAGCGCA GAGGATGTAC AAGCAGTCGA TGGCGCAGAG AGCGCGGACC 
ATGGCCCTCT ACAACCCCAT CCCTGTCCGC CAGAACTGCC TCACGGTCAA CCGCTCCCTG 
TTCCTCTTCA GTGAAGACAA CGTGGTGAGA AAATACGCCA AAAAGATCAC GGAATGGCCT 
CCCTTCGAGT ACATGATCCT GGCCACCATC ATTGCTAACT GCATCGTCCT GGCCCTGGAG 
CAGCACCTCC CTGATGATGA CAAGACACCC ATGTCCGAGC GGCTGGATGA CACAGAACCC 
TATTTCATTG GCATCTTCTG TTTTGAGGCT GGAATTAAGA TCGTGGCTCT TGGCTTTGCC 
TTCCACAAAG GCTCCTACCT GAGGAATGGC TGGAACGTCA TGGACTTTGT CGTGGTGCTA 
ACAGGCATCT TGGCCACTGT CGGGACGGAG TTTGATCTAC GGACACTGAG GGCGGTTCGT 
GTGCTGCGGC CACTCAAGCT GGTGTCTGGA ATCCCAAGTT TACAAGTCGT CCTGAAGTCA 
ATCATGAAGG CGATGATCCC TCTGCTGCAG ATCGGCCTCC TCCTGTTTTT TGCAATCCTT 
ATTTTTGCAA TCATAGGGTT AGAATTTTAT ATGGGAAAAT TTCATACCAC CTGCTTTGAA 
GAGGGGACAG ACGACATCCA GGGTGAGTCG CCAGCTCCGT GTGGGACAGA GGAGCCTGCC 
CGCACCTGCC CCAACGGGAC CAAATGTCAG CCGTACTGGG AAGGGCCCAA CAACGGCATC 
ACTCAGTTCG ACAACATCCT GTTTGCTGTG CTCACTGTTT TCCAGTGCAT CACCATGGAA 
GGCTGGACTG ATCTCCTCTA CAATAGCAAC GATGCCTCAG GGAACACTTG GAACTGGTTG 
TACTTCATCC CCCTCATCAT CATCGGCTCC TTTTTTATGC TGAACCTTGT GCTGGGTGTG 
CTGTCTGGGG AGTTTGCCAA AGAAAGGGAA CGTGTAGAGA ACCGAAGGGC TTTTCTGAAG 
CTCAGAAGAC AACAGCAGAT TGAACGTGAG CTCAATGGAT ACATGGAGTG GATCTCGAAA 
GCAGAAGAGG TGATCCTCGC GGAGGACGAG ACAGACGTGG AGCAGAGGCA CCCTTTTGAT 
GGAGCTCTTC GGAGAGCTAC TCTGAAGAAA AGCAAGACGG ACCTGCTCAA CCCTGAGGAG 
GCGGAGGACC AGCTTGCTGA CATCGCGTCT GTGGGGTCTC CCTTCGCCAG AGCCAGCATC 
AAAAGTGCCA AGCTGGAGAA TTCGACTTTT TTCCACAAAA AGGAGAGAAG AATGCGTTTC 
TACATCCGCC GCATGGTCAA AACTCAGGCC TTCTACTGGA CCGTGCTCAG TCTGGTAGCC 
CTCAACACGC TGTGGCTCGC CATTGTCCAC TACAACCAGC CCGAGTGGCT CTCCGACTTC 
CTCTACTATG CAGAATTCAT TTTCTTAGGA CTCTTTATGT CCGAAATGTT TATAAAAATG 
TATGGGCTCG GGACACGGCC TTACTTCCAC TCTTCCTTCA ACTGCTTTGA CTGTGGGGTC 
ATCATCGGGA GCATCTTTGA AGTCATCTGG GCCGTCATCA AACCGGGTAC ATCCTTTGGA 
ATCAGCGTGT TACGAGCTCT CAGGTTACTG CGTATTTTCA AAGTCACAAA GTACTGGGCA 
TCTCTCAGAA ACCTGGTTGT CTCCCTCCTC AACTCCATGA AATCCATCAT AAGTCTGCTG 
TTCCTCCTCT TCCTCTTCAT TGTCGTCTTT GCCCTCTTGG GGATGCAGCT GTTTGGTGGC 
CAGTTTAATT TTGACGAGGG GACTCCTCCC ACCAACTTCG ACACTTTTCC AGCAGCAATA 
ATGACTGTGT TTCAGATCCT GACTGGCGAG GATTGGAATG AGGTCATGTA TGATGAGATC 
AAGTCTCAGG GGGGCGTGCA GGGCGGCATG GTGTTCTCCA TCTACTTCAT CGTCCTCACC 
CTCTTCGGGA ACTACACCCT GCTGAACGTG TTCTTAGCTA TCGCGGTGGA CAACCTGGCC 
AACGCCCAGG AACTCACCAA GGATGAACAA GAAGAGGAAG AGGCAGCCAA TCAGAAACTG 
GCTCTACAGA AAGCCAAGGA GGTGGCAGAA GTGAGTCCCC TGTCTGCAGC CAACATGTCC 
ATAGCTGTGA AGGAACAGCA GAAGAACCAG AAGCCTGCCA AGTCGGTGTG GGAGCAGCGC 
ACCAGCGAGA TGCGCAAGCA GAACCTGCTG GCTAGCCGCG AGGCGCTGTA CGGGGACGCG 
GCTGAGCGCT GGCCCACCAC TTACGCGCGC CCGCTGCGGC CGGACGTGAA GACGCACTTG 
GACCGGCCGC TCGTGGTGGA CCCGCAGGAG AACCGTAACA ACAACACCAA CAAGAGCCGT 
GCGCCAGAAG CGCTGCGCCA AACCGCGCGG CCCCGCGAGA GCGCGCGCGA CCCCGACGCG 
CGGCGCGCCT GGCCCAGCAG CCCTGAGCGC GCCCCTGGAC GAGAGGGCCC GTATGGCCGC 
GAGAGCGAGC CGCAACAGCG CGAGCACGCG CCACCCCGCG AGCACGTACC CTGGGACGCG 
GATCCTGAGC GCGCCAAGGC CGGGGACGCG CCCCGCCGCC ACACGCACCG GCCTGTGGCC 
GAGGGCGAGC CTCGTCGCCA CCGCGCGCGC CGCCGGCCCG GGGACGAACC GGACGACAGA 
CCGGAGCGCA GGCCGCGTCC CCGCGACGCC ACTAGGCCGG CCCGCGCTGC AGACGGCGAA 
GGCGATGATG GGGAGCGCAA GCGGCGACAC CGACACGGGC CGCCGGCCCA CGATGACAGG 
GAGCGCAGAC ACCGGCGCAG AAAAGAGAGC CAGGGCTCTG GGGTCCCCAT GTCTGGTCCC 
AACCTGTCCA CCACCAGGCC AATCCAGCAG GATCTGGGCC GCCAGGACCT GCCACTGGCT 
GAGGACCTGG ACAACATGAA GAACAACAAG TTGGCCACCG GGGAGCCTGC CAGTCCCCAC 
GACAGCCTGG GCCACAGTGG CCTTCCCCCT AGCCCTGCCA AGATCGGGAA CAGCACCAAC 
CCTGGTCCCG CCTTGGCCAC CAATCCCCAG AATGCTGCCA GCCGCAGGAC GCCCAACAAC 
CCGGGCAACC CGTCCAACCC CGGCCCCCCC AAGACTCCCG AGAACAGCCT TATCGTCACC 
AACCCCAGCA GCACCCAGCC CAACTCAGCA AAGACTGCCA GGAAACCCGA GCACATGGCG 
GTGGAGATCC CCCCGGCCTG CCCGCCCCTC AACCACACTG TGGTCCAAGT AAACAAAAAC 
GCCAACCCAG ACCCACTGCC AAAGAAAGAG GAAGAGAAGA AGGAGGAAGA GGAGGCAGAC 
CCCGGGGAGG ATGGCCCAAA GCCCATGCCG CCCTACAGCT CCATGTTCAT CCTCTCCACC 
ACCAACCCCC TTCGCCGGCT GTGCCATTAC ATCCTGAACC TGCGCTACTT CGAGATGTGC 
ATCCTCATGG TCATTGCCAT GAGTAGCATC GCGCTGGCCG CCGAGGACCC GGTGCAGCCC 
AACGCACCCC GCAACAACGT GCTGCGATAT TTTGACTATG TTTTCACAGG AGTGTTTACC 
TTTGAGATGG TGATCAAGAT GATCGACCTG GGCCTCGTCC TGCATCAGGG GGCCTATTTC 
CGTGACCTGT GGAACATTCT GGACTTCATA GTGGTCAGTG GGGCCCTGGT GGCCTTTGCC 
TTCACTGGCA ATAGCAAAGG AAAGGACATC AACACCATCA AGTCCCTCCG AGTCCTCCGG 
GTGCTACGAC CTCTAAAGAC CATCAAGCGG CTGCCTAAGT TGAAGGCCGT ATTTGACTGC 
GTGGTGAACT CGCTCAAGAA CGTCTTCAAC ATCCTCATTG TCTACATGCT CTTCATGTTC 
ATCTTCGCCG TGGTGGCCGT GCAGCTCTTC AAGGGCAAAT TCTTCCACTG CACGGACGAG 
TCCAAGGAGT TTGAGAGAGA CTGTCGAGGC AAATACCTCC TTTACGAGAA GAACGAGGTA 
AAGGCGCGGG ACCGCGAGTG GAAGAAATAC GACTTCCACT ACGACAACGT GCTCTGGGCC 
CTGCTCACGC TCTTTACGGT GTCCACGGGA GAGGGCTGGC CACAGGTCCT CAAGCACTCA 
GTGGATGCCA CTTTTGAGAA CCAGGGCCCC AGCCCCGGGT ACCGCATGGA AATGTCCATC 
TTCTACGTGG TCTACTTTGT GGTGTTTCCC TTCTTCTTTG TCAATATCTT TGTGGCCTTG 
ATCATCATCA CCTTCCAGGA GCAGGGAGAC AAGATGATGG AAGAATACAG CCTAGAGAAA 
AATGAGAGGG CCTGCATCGA CTTTGCCATC AGTGCCAAGC CGCTGACCAG GCACATGCCC 
CAGAACAAGC AGAGCTTCCA GTATCGAATG TGGCAGTTCG TGGTGTCCCC ACCCTTTGAG 
TACACCATCA TGGCCATGAT CGCTCTCAAC ACCATCGTGC TAATGATGAA GTTCTATGGA 
GCCTCTGTGG CCTATGAAAA CGCCCTTCGA GTGTTCAACA TTGTCTTCAC CTCCCTCTTC 
TCTCTCGAAT GTGTGCTCAA AGTCATGGCT TTTGGGATTC TGAATTATTT CCGCGATGCC 
TGGAACATCT TCGACTTTGT GACTGTTCTG GGCAGCATCA CAGACATCCT CGTCACCGAG 
TTTGGGAATA ACTTCATCAA CCTGAGCTTT CTCCGCCTCT TCCGTGCTGC CCGACTCATC 
AAACTCCTCC GCCAGGGTTA CACCATCCGC ATTCTCCTCT GGACTTTCGT GCAGTCTTTC 
AAGGCCCTAC CTTATGTCTG TCTGCTGATC GCCATGCTCT TCTTCATCTA TGCCATCATC 
GGGATGCAGG TGTTTGGCAA CATCGGCATT GATGGGGAAG ATGAGGACAG CGATGAGGAT 
GAGTTCCAAA TCACGGAGCA CAATAACTTC CGGACCTTCT TCCAAGCTCT CATGCTTCTC 
TTCCGGAGCG CCACAGGGGA AGCGTGGCAC AACATCATGC TGTCCTGCCT CAGCGGGAAG 
CCATGCGACA AGAACTCCGG GATCCAAAAA CCAGAGTGTG GCAACGAGTT CGCCTATTTT 
TACTTTGTCT CGTTCATCTT CCTTTGCTCA TTTCTGATGC TGAATCTCTT TGTTGCTGTC 
ATCATGGACA ACTTCGAGTA CCTCACCCGA GATTCCTCCA TCCTGGGCCC CCACCACCTG 
GATGAGTACG TGCGTGTCTG GGCAGAGTAT GACCCTGCTG CCTGCGGCCG CATTCACTAT 
AAGGACATGT ACAGTTTATT GCGAGTAATA TCGCCCCCTC TCGGCTTAGG CAAGAAATGT 
CCTCATAGGG TTGCTTGCAA GAGGCTCTTG CGGATGGACC TACCCGTAGC GGATGACAAC 
ACCGTTCACT TCAACTCCAC CTTGATGGCT CTGATCCGAA CCGCCCTGGA TATCAAAATC 
GCAAAGGGTG GAGCTGACAA GCAGCAAATG GACGCAGAGC TCCGCAAGGA AATGATGGCC 
ATTTGGCCCA ACCTGTCTCA GAAGACCTTG GATCTGCTGG TCACACCTCA CAAGTCCACG 
GACCTGACAG TGGGTAAGAT CTACGCAGCC ATGATGATCA TGGAGTACTA CCGGCAGAGC 
AAGGCCAAGA AGCTGCAGGC CATGCGAGAG GAGCAGAACC GGACACCACT CATGTTCCAG 
CGCATGGAGC CTCCATCGCC AACACAGGAG GGAGGACCCA GCCAAAACGC CCTTCCCTCC 
ACTCAGCTGG ACCCCGGAGG AGGCCTGATG GCTCAAGAAA GCAGCATGAA GGAGAGCCCG 
TCCTGGGTGA CCCAGCGGGC ACAGGAGATG TTCCAGAAGA CTGGTACCTG GAGCCCAGAG 
CGAGGGCCAC CCATCGACAT GCCTAACAGC CAGCCCAACT CCCAGTCTGT GGAGATGAGA 
GAAATGGGAA CTGATGGCTA CTCAGACAGC GAACACTACC TCCCCATGGA AGGACAGACC 
AGGGCCGCCT CCATGCCCCG CCTCCCAGCA GAGAACCAGA GGAGAAGGGG CCGGCCACGT 
GGAAATAACC TCAGTACCAT CTCTGATACC AGCCCCATGA AGCGCTCAGC CTCCGTGCTG 
GGACCCAAAG CCCGGCGACT GGATGACTAC TCACTAGAGC GGGTACCACC TGAGGAGAAC 
CAAAGGTACC ACCAACGCCG CCGGGACCGT GGCCACCGCA CCTCTGAGCG CTCTCTGGGC 
CGATACACTG ATGTGGACAC AGGCCTGGGG ACAGATCTGA GCATGACCAC CCAATCGGGT 
GACCTGCCCT CCAAAGATCG GGACCAGGAC CGGGGCCGGC CCAAGGACCG GAAGCATCGG 
CCACACCACC ACCACCACCA TCATCACCAT CATCCCCCGG CCCCGGACCG GGAGCGCTAC 
GCACAGGAGC GGCCGGACAC CGGCCGGGCG CGGGCCCGGG AGCAGCGCTG GTCCCGCTCG 
CCCAGCGAGG GTCGGGAGCA CGCGACACAC AGACAGGGCA GTAGTTCCGT CAGTGGAAGC 
CCAGCCCCCT CTACGTCCGG CACCAGCACG CCGCGGCGCG GCCGCCGCCA GCTCCCCCAG 
ACCCCCTGCA CCCCGCGGCC GCTTGTGTCC TACTCGCCCG CTCCGCGCAG GCCTGCGGCG 
CGCAGGATGG CGGGTCCCCC AGCGCCCCCT GGCGGCTCGC CGCGGGGTTG CCGCCGTGCG 
CCGCGCTGGC CTGCGCATGC GCCTGAGGGC CCGCGGCCGC GCGGTGCCGA CTACACCGAG 
CCCGACAGCC CGCGCGAGCC GCCAGGGGGC GCGCATGAGC CCGCGCCGCG GTCGCCCAGG 
ACTCCGCGCG CCGCGGGCTG CGCGTCACCG CGCCACGGCC GCAGGCTGCC CAATGGTTAC 
TACGCGGGGC ACGGCGCGCC ACGGCCGCGC ACAGCCCGCA GGGGCGCGCA CGACGCGTAC 
AGCGAGAGCG AGGACGACTG GTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037050.2
CDS	1..7107
Translation	MARFGDEMPGRYGAGGGGSGPAAGVVVGAAGGRGAGGSRQGGQPGAQRMYKQSMAQRARTMALYNPIPVRQNCLTVNRSLFLFSEDNVVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDDDKTPMSERLDDTEPYFIGIFCFEAGIKIVALGFAFHKGSYLRNGWNVMDFVVVLTGILATVGTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILIFAIIGLEFYMGKFHTTCFEEGTDDIQGESPAPCGTEEPARTCPNGTKCQPYWEGPNNGITQFDNILFAVLTVFQCITMEGWTDLLYNSNDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYMEWISKAEEVILAEDETDVEQRHPFDGALRRATLKKSKTDLLNPEEAEDQLADIASVGSPFARASIKSAKLENSTFFHKKERRMRFYIRRMVKTQAFYWTVLSLVALNTLWLAIVHYNQPEWLSDFLYYAEFIFLGLFMSEMFIKMYGLGTRPYFHSSFNCFDCGVIIGSIFEVIWAVIKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFDEGTPPTNFDTFPAAIMTVFQILTGEDWNEVMYDEIKSQGGVQGGMVFSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAANQKLALQKAKEVAEVSPLSAANMSIAVKEQQKNQKPAKSVWEQRTSEMRKQNLLASREALYGDAAERWPTTYARPLRPDVKTHLDRPLVVDPQENRNNNTNKSRAPEALRQTARPRESARDPDARRAWPSSPERAPGREGPYGRESEPQQREHAPPREHVPWDADPERAKAGDAPRRHTHRPVAEGEPRRHRARRRPGDEPDDRPERRPRPRDATRPARAADGEGDDGERKRRHRHGPPAHDDRERRHRRRKESQGSGVPMSGPNLSTTRPIQQDLGRQDLPLAEDLDNMKNNKLATGEPASPHDSLGHSGLPPSPAKIGNSTNPGPALATNPQNAASRRTPNNPGNPSNPGPPKTPENSLIVTNPSSTQPNSAKTARKPEHMAVEIPPACPPLNHTVVQVNKNANPDPLPKKEEEKKEEEEADPGEDGPKPMPPYSSMFILSTTNPLRRLCHYILNLRYFEMCILMVIAMSSIALAAEDPVQPNAPRNNVLRYFDYVFTGVFTFEMVIKMIDLGLVLHQGAYFRDLWNILDFIVVSGALVAFAFTGNSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYMLFMFIFAVVAVQLFKGKFFHCTDESKEFERDCRGKYLLYEKNEVKARDREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATFENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEEYSLEKNERACIDFAISAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYTIMAMIALNTIVLMMKFYGASVAYENALRVFNIVFTSLFSLECVLKVMAFGILNYFRDAWNIFDFVTVLGSITDILVTEFGNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIGIDGEDEDSDEDEFQITEHNNFRTFFQALMLLFRSATGEAWHNIMLSCLSGKPCDKNSGIQKPECGNEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRVWAEYDPAACGRIHYKDMYSLLRVISPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKQQMDAELRKEMMAIWPNLSQKTLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKKLQAMREEQNRTPLMFQRMEPPSPTQEGGPSQNALPSTQLDPGGGLMAQESSMKESPSWVTQRAQEMFQKTGTWSPERGPPIDMPNSQPNSQSVEMREMGTDGYSDSEHYLPMEGQTRAASMPRLPAENQRRRGRPRGNNLSTISDTSPMKRSASVLGPKARRLDDYSLERVPPEENQRYHQRRRDRGHRTSERSLGRYTDVDTGLGTDLSMTTQSGDLPSKDRDQDRGRPKDRKHRPHHHHHHHHHHPPAPDRERYAQERPDTGRARAREQRWSRSPSEGREHATHRQGSSSVSGSPAPSTSGTSTPRRGRRQLPQTPCTPRPLVSYSPAPRRPAARRMAGPPAPPGGSPRGCRRAPRWPAHAPEGPRPRGADYTEPDSPREPPGGAHEPAPRSPRTPRAAGCASPRHGRRLPNGYYAGHGAPRPRTARRGAHDAYSESEDDWC

Target ORF information:

RefSeq Version	NM_012918.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel subunit alpha1 A (Cacna1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012918.3

ORF Insert Sequence:

ATGGCCCGCT TTGGAGACGA GATGCCGGGC CGCTACGGCG CAGGCGGAGG AGGCTCAGGG 
CCGGCCGCCG GGGTGGTCGT GGGCGCCGCG GGCGGCCGAG GAGCCGGGGG CAGCCGGCAG 
GGCGGGCAGC CCGGAGCGCA GAGGATGTAC AAGCAGTCGA TGGCGCAGAG AGCGCGGACC 
ATGGCCCTCT ACAACCCCAT CCCTGTCCGC CAGAACTGCC TCACGGTCAA CCGCTCCCTG 
TTCCTCTTCA GTGAAGACAA CGTGGTGAGA AAATACGCCA AAAAGATCAC GGAATGGCCT 
CCCTTCGAGT ACATGATCCT GGCCACCATC ATTGCTAACT GCATCGTCCT GGCCCTGGAG 
CAGCACCTCC CTGATGATGA CAAGACACCC ATGTCCGAGC GGCTGGATGA CACAGAACCC 
TATTTCATTG GCATCTTCTG TTTTGAGGCT GGAATTAAGA TCGTGGCTCT TGGCTTTGCC 
TTCCACAAAG GCTCCTACCT GAGGAATGGC TGGAACGTCA TGGACTTTGT CGTGGTGCTA 
ACAGGCATCT TGGCCACTGT CGGGACGGAG TTTGATCTAC GGACACTGAG GGCGGTTCGT 
GTGCTGCGGC CACTCAAGCT GGTGTCTGGA ATCCCAAGTT TACAAGTCGT CCTGAAGTCA 
ATCATGAAGG CGATGATCCC TCTGCTGCAG ATCGGCCTCC TCCTGTTTTT TGCAATCCTT 
ATTTTTGCAA TCATAGGGTT AGAATTTTAT ATGGGAAAAT TTCATACCAC CTGCTTTGAA 
GAGGGGACAG ACGACATCCA GGGTGAGTCG CCAGCTCCGT GTGGGACAGA GGAGCCTGCC 
CGCACCTGCC CCAACGGGAC CAAATGTCAG CCGTACTGGG AAGGGCCCAA CAACGGCATC 
ACTCAGTTCG ACAACATCCT GTTTGCTGTG CTCACTGTTT TCCAGTGCAT CACCATGGAA 
GGCTGGACTG ATCTCCTCTA CAATAGCAAC GATGCCTCAG GGAACACTTG GAACTGGTTG 
TACTTCATCC CCCTCATCAT CATCGGCTCC TTTTTTATGC TGAACCTTGT GCTGGGTGTG 
CTGTCTGGGG AGTTTGCCAA AGAAAGGGAA CGTGTAGAGA ACCGAAGGGC TTTTCTGAAG 
CTCAGAAGAC AACAGCAGAT TGAACGTGAG CTCAATGGAT ACATGGAGTG GATCTCGAAA 
GCAGAAGAGG TGATCCTCGC GGAGGACGAG ACAGACGTGG AGCAGAGGCA CCCTTTTGAT 
GGAGCTCTTC GGAGAGCTAC TCTGAAGAAA AGCAAGACGG ACCTGCTCAA CCCTGAGGAG 
GCGGAGGACC AGCTTGCTGA CATCGCGTCT GTGGGGTCTC CCTTCGCCAG AGCCAGCATC 
AAAAGTGCCA AGCTGGAGAA TTCGACTTTT TTCCACAAAA AGGAGAGAAG AATGCGTTTC 
TACATCCGCC GCATGGTCAA AACTCAGGCC TTCTACTGGA CCGTGCTCAG TCTGGTAGCC 
CTCAACACGC TGTGGCTCGC CATTGTCCAC TACAACCAGC CCGAGTGGCT CTCCGACTTC 
CTCTACTATG CAGAATTCAT TTTCTTAGGA CTCTTTATGT CCGAAATGTT TATAAAAATG 
TATGGGCTCG GGACACGGCC TTACTTCCAC TCTTCCTTCA ACTGCTTTGA CTGTGGGGTC 
ATCATCGGGA GCATCTTTGA AGTCATCTGG GCCGTCATCA AACCGGGTAC ATCCTTTGGA 
ATCAGCGTGT TACGAGCTCT CAGGTTACTG CGTATTTTCA AAGTCACAAA GTACTGGGCA 
TCTCTCAGAA ACCTGGTTGT CTCCCTCCTC AACTCCATGA AATCCATCAT AAGTCTGCTG 
TTCCTCCTCT TCCTCTTCAT TGTCGTCTTT GCCCTCTTGG GGATGCAGCT GTTTGGTGGC 
CAGTTTAATT TTGACGAGGG GACTCCTCCC ACCAACTTCG ACACTTTTCC AGCAGCAATA 
ATGACTGTGT TTCAGATCCT GACTGGCGAG GATTGGAATG AGGTCATGTA TGATGAGATC 
AAGTCTCAGG GGGGCGTGCA GGGCGGCATG GTGTTCTCCA TCTACTTCAT CGTCCTCACC 
CTCTTCGGGA ACTACACCCT GCTGAACGTG TTCTTAGCTA TCGCGGTGGA CAACCTGGCC 
AACGCCCAGG AACTCACCAA GGATGAACAA GAAGAGGAAG AGGCAGCCAA TCAGAAACTG 
GCTCTACAGA AAGCCAAGGA GGTGGCAGAA GTGAGTCCCC TGTCTGCAGC CAACATGTCC 
ATAGCTGTGA AGGAACAGCA GAAGAACCAG AAGCCTGCCA AGTCGGTGTG GGAGCAGCGC 
ACCAGCGAGA TGCGCAAGCA GAACCTGCTG GCTAGCCGCG AGGCGCTGTA CGGGGACGCG 
GCTGAGCGCT GGCCCACCAC TTACGCGCGC CCGCTGCGGC CGGACGTGAA GACGCACTTG 
GACCGGCCGC TCGTGGTGGA CCCGCAGGAG AACCGTAACA ACAACACCAA CAAGAGCCGT 
GCGCCAGAAG CGCTGCGCCA AACCGCGCGG CCCCGCGAGA GCGCGCGCGA CCCCGACGCG 
CGGCGCGCCT GGCCCAGCAG CCCTGAGCGC GCCCCTGGAC GAGAGGGCCC GTATGGCCGC 
GAGAGCGAGC CGCAACAGCG CGAGCACGCG CCACCCCGCG AGCACGTACC CTGGGACGCG 
GATCCTGAGC GCGCCAAGGC CGGGGACGCG CCCCGCCGCC ACACGCACCG GCCTGTGGCC 
GAGGGCGAGC CTCGTCGCCA CCGCGCGCGC CGCCGGCCCG GGGACGAACC GGACGACAGA 
CCGGAGCGCA GGCCGCGTCC CCGCGACGCC ACTAGGCCGG CCCGCGCTGC AGACGGCGAA 
GGCGATGATG GGGAGCGCAA GCGGCGACAC CGACACGGGC CGCCGGCCCA CGATGACAGG 
GAGCGCAGAC ACCGGCGCAG AAAAGAGAGC CAGGGCTCTG GGGTCCCCAT GTCTGGTCCC 
AACCTGTCCA CCACCAGGCC AATCCAGCAG GATCTGGGCC GCCAGGACCT GCCACTGGCT 
GAGGACCTGG ACAACATGAA GAACAACAAG TTGGCCACCG GGGAGCCTGC CAGTCCCCAC 
GACAGCCTGG GCCACAGTGG CCTTCCCCCT AGCCCTGCCA AGATCGGGAA CAGCACCAAC 
CCTGGTCCCG CCTTGGCCAC CAATCCCCAG AATGCTGCCA GCCGCAGGAC GCCCAACAAC 
CCGGGCAACC CGTCCAACCC CGGCCCCCCC AAGACTCCCG AGAACAGCCT TATCGTCACC 
AACCCCAGCA GCACCCAGCC CAACTCAGCA AAGACTGCCA GGAAACCCGA GCACATGGCG 
GTGGAGATCC CCCCGGCCTG CCCGCCCCTC AACCACACTG TGGTCCAAGT AAACAAAAAC 
GCCAACCCAG ACCCACTGCC AAAGAAAGAG GAAGAGAAGA AGGAGGAAGA GGAGGCAGAC 
CCCGGGGAGG ATGGCCCAAA GCCCATGCCG CCCTACAGCT CCATGTTCAT CCTCTCCACC 
ACCAACCCCC TTCGCCGGCT GTGCCATTAC ATCCTGAACC TGCGCTACTT CGAGATGTGC 
ATCCTCATGG TCATTGCCAT GAGTAGCATC GCGCTGGCCG CCGAGGACCC GGTGCAGCCC 
AACGCACCCC GCAACAACGT GCTGCGATAT TTTGACTATG TTTTCACAGG AGTGTTTACC 
TTTGAGATGG TGATCAAGAT GATCGACCTG GGCCTCGTCC TGCATCAGGG GGCCTATTTC 
CGTGACCTGT GGAACATTCT GGACTTCATA GTGGTCAGTG GGGCCCTGGT GGCCTTTGCC 
TTCACTGGCA ATAGCAAAGG AAAGGACATC AACACCATCA AGTCCCTCCG AGTCCTCCGG 
GTGCTACGAC CTCTAAAGAC CATCAAGCGG CTGCCTAAGT TGAAGGCCGT ATTTGACTGC 
GTGGTGAACT CGCTCAAGAA CGTCTTCAAC ATCCTCATTG TCTACATGCT CTTCATGTTC 
ATCTTCGCCG TGGTGGCCGT GCAGCTCTTC AAGGGCAAAT TCTTCCACTG CACGGACGAG 
TCCAAGGAGT TTGAGAGAGA CTGTCGAGGC AAATACCTCC TTTACGAGAA GAACGAGGTA 
AAGGCGCGGG ACCGCGAGTG GAAGAAATAC GACTTCCACT ACGACAACGT GCTCTGGGCC 
CTGCTCACGC TCTTTACGGT GTCCACGGGA GAGGGCTGGC CACAGGTCCT CAAGCACTCA 
GTGGATGCCA CTTTTGAGAA CCAGGGCCCC AGCCCCGGGT ACCGCATGGA AATGTCCATC 
TTCTACGTGG TCTACTTTGT GGTGTTTCCC TTCTTCTTTG TCAATATCTT TGTGGCCTTG 
ATCATCATCA CCTTCCAGGA GCAGGGAGAC AAGATGATGG AAGAATACAG CCTAGAGAAA 
AATGAGAGGG CCTGCATCGA CTTTGCCATC AGTGCCAAGC CGCTGACCAG GCACATGCCC 
CAGAACAAGC AGAGCTTCCA GTATCGAATG TGGCAGTTCG TGGTGTCCCC ACCCTTTGAG 
TACACCATCA TGGCCATGAT CGCTCTCAAC ACCATCGTGC TAATGATGAA GTTCTATGGA 
GCCTCTGTGG CCTATGAAAA CGCCCTTCGA GTGTTCAACA TTGTCTTCAC CTCCCTCTTC 
TCTCTCGAAT GTGTGCTCAA AGTCATGGCT TTTGGGATTC TGAATTATTT CCGCGATGCC 
TGGAACATCT TCGACTTTGT GACTGTTCTG GGCAGCATCA CAGACATCCT CGTCACCGAG 
TTTGGGAATA ACTTCATCAA CCTGAGCTTT CTCCGCCTCT TCCGTGCTGC CCGACTCATC 
AAACTCCTCC GCCAGGGTTA CACCATCCGC ATTCTCCTCT GGACTTTCGT GCAGTCTTTC 
AAGGCCCTAC CTTATGTCTG TCTGCTGATC GCCATGCTCT TCTTCATCTA TGCCATCATC 
GGGATGCAGG TGTTTGGCAA CATCGGCATT GATGGGGAAG ATGAGGACAG CGATGAGGAT 
GAGTTCCAAA TCACGGAGCA CAATAACTTC CGGACCTTCT TCCAAGCTCT CATGCTTCTC 
TTCCGGAGCG CCACAGGGGA AGCGTGGCAC AACATCATGC TGTCCTGCCT CAGCGGGAAG 
CCATGCGACA AGAACTCCGG GATCCAAAAA CCAGAGTGTG GCAACGAGTT CGCCTATTTT 
TACTTTGTCT CGTTCATCTT CCTTTGCTCA TTTCTGATGC TGAATCTCTT TGTTGCTGTC 
ATCATGGACA ACTTCGAGTA CCTCACCCGA GATTCCTCCA TCCTGGGCCC CCACCACCTG 
GATGAGTACG TGCGTGTCTG GGCAGAGTAT GACCCTGCTG CCTGCGGCCG CATTCACTAT 
AAGGACATGT ACAGTTTATT GCGAGTAATA TCGCCCCCTC TCGGCTTAGG CAAGAAATGT 
CCTCATAGGG TTGCTTGCAA GAGGCTCTTG CGGATGGACC TACCCGTAGC GGATGACAAC 
ACCGTTCACT TCAACTCCAC CTTGATGGCT CTGATCCGAA CCGCCCTGGA TATCAAAATC 
GCAAAGGGTG GAGCTGACAA GCAGCAAATG GACGCAGAGC TCCGCAAGGA AATGATGGCC 
ATTTGGCCCA ACCTGTCTCA GAAGACCTTG GATCTGCTGG TCACACCTCA CAAGTCCACG 
GACCTGACAG TGGGTAAGAT CTACGCAGCC ATGATGATCA TGGAGTACTA CCGGCAGAGC 
AAGGCCAAGA AGCTGCAGGC CATGCGAGAG GAGCAGAACC GGACACCACT CATGTTCCAG 
CGCATGGAGC CTCCATCGCC AACACAGGAG GGAGGACCCA GCCAAAACGC CCTTCCCTCC 
ACTCAGCTGG ACCCCGGAGG AGGCCTGATG GCTCAAGAAA GCAGCATGAA GGAGAGCCCG 
TCCTGGGTGA CCCAGCGGGC ACAGGAGATG TTCCAGAAGA CTGGTACCTG GAGCCCAGAG 
CGAGGGCCAC CCATCGACAT GCCTAACAGC CAGCCCAACT CCCAGTCTGT GGAGATGAGA 
GAAATGGGAA CTGATGGCTA CTCAGACAGC GAACACTACC TCCCCATGGA AGGACAGACC 
AGGGCCGCCT CCATGCCCCG CCTCCCAGCA GAGAACCAGA GGAGAAGGGG CCGGCCACGT 
GGAAATAACC TCAGTACCAT CTCTGATACC AGCCCCATGA AGCGCTCAGC CTCCGTGCTG 
GGACCCAAAG CCCGGCGACT GGATGACTAC TCACTAGAGC GGGTACCACC TGAGGAGAAC 
CAAAGGTACC ACCAACGCCG CCGGGACCGT GGCCACCGCA CCTCTGAGCG CTCTCTGGGC 
CGATACACTG ATGTGGACAC AGGCCTGGGG ACAGATCTGA GCATGACCAC CCAATCGGGT 
GACCTGCCCT CCAAAGATCG GGACCAGGAC CGGGGCCGGC CCAAGGACCG GAAGCATCGG 
CCACACCACC ACCACCACCA TCATCACCAT CATCCCCCGG CCCCGGACCG GGAGCGCTAC 
GCACAGGAGC GGCCGGACAC CGGCCGGGCG CGGGCCCGGG AGCAGCGCTG GTCCCGCTCG 
CCCAGCGAGG GTCGGGAGCA CGCGACACAC AGACAGGGCA GTAGTTCCGT CAGTGGAAGC 
CCAGCCCCCT CTACGTCCGG CACCAGCACG CCGCGGCGCG GCCGCCGCCA GCTCCCCCAG 
ACCCCCTGCA CCCCGCGGCC GCTTGTGTCC TACTCGCCCG CTCCGCGCAG GCCTGCGGCG 
CGCAGGATGG CGGGTCCCCC AGCGCCCCCT GGCGGCTCGC CGCGGGGTTG CCGCCGTGCG 
CCGCGCTGGC CTGCGCATGC GCCTGAGGGC CCGCGGCCGC GCGGTGCCGA CTACACCGAG 
CCCGACAGCC CGCGCGAGCC GCCAGGGGGC GCGCATGAGC CCGCGCCGCG GTCGCCCAGG 
ACTCCGCGCG CCGCGGGCTG CGCGTCACCG CGCCACGGCC GCAGGCTGCC CAATGGTTAC 
TACGCGGGGC ACGGCGCGCC ACGGCCGCGC ACAGCCCGCA GGGGCGCGCA CGACGCGTAC 
AGCGAGAGCG AGGACGACTG GTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1a ( XM_017601177.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017456666.1 Rattus norvegicus calcium voltage-gated channel subunit alpha1 A (Cacna1a), transcript variant X1, mRNA.

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CloneID	ORa59364
Clone ID Related Accession (Same CDS sequence)	XM_017601177.1
Accession Version	XM_017601177.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7539bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTCCT CTCCTGGGAT TTCTGAGCGA GGCCCATGGA TGCCGAGGGC CTCCTGGGTC 
TCTGACCCCA TGCGTTCTCC CCTCAATCAT GAGCTCCTGC ACAGCTCGAG GTACCCAGGC 
ACGGACTGCA CCCTGGCAGG TTCTTCCAGG ACTTTGCATG TCCCGTGGTC CGAGCAGATG 
AGCGGAAGCC TCGAGGGGCC AACTCTGAAA CGGCGTGGTG GCGTGGTGGA CCACCGGGAT 
GTCATCTTGG CCCACCAGGC GCACAAAATG CACAGCACCC CTCAGGCCAG GAGGAAAGAG 
TGGGAAATGG CCCGCTTTGG AGACGAGATG CCGGGCCGCT ACGGCGCAGG CGGAGGAGGC 
TCAGGGCCGG CCGCCGGGGT GGTCGTGGGC GCCGCGGGCG GCCGAGGAGC CGGGGGCAGC 
CGGCAGGGCG GGCAGCCCGG AGCGCAGAGG ATGTACAAGC AGTCGATGGC GCAGAGAGCG 
CGGACCATGG CCCTCTACAA CCCCATCCCT GTCCGCCAGA ACTGCCTCAC GGTCAACCGC 
TCCCTGTTCC TCTTCAGTGA AGACAACGTG GTGAGAAAAT ACGCCAAAAA GATCACGGAA 
TGGCCTCCCT TCGAGTACAT GATCCTGGCC ACCATCATTG CTAACTGCAT CGTCCTGGCC 
CTGGAGCAGC ACCTCCCTGA TGATGACAAG ACACCCATGT CCGAGCGGCT GGATGACACA 
GAACCCTATT TCATTGGCAT CTTCTGTTTT GAGGCTGGAA TTAAGATCGT GGCTCTTGGC 
TTTGCCTTCC ACAAAGGCTC CTACCTGAGG AATGGCTGGA ACGTCATGGA CTTTGTCGTG 
GTGCTAACAG GCATCTTGGC CACTGTCGGG ACGGAGTTTG ATCTACGGAC ACTGAGGGCG 
GTTCGTGTGC TGCGGCCACT CAAGCTGGTG TCTGGAATCC CAAGTTTACA AGTCGTCCTG 
AAGTCAATCA TGAAGGCGAT GATCCCTCTG CTGCAGATCG GCCTCCTCCT GTTTTTTGCA 
ATCCTTATTT TTGCAATCAT AGGGTTAGAA TTTTATATGG GAAAATTTCA TACCACCTGC 
TTTGAAGAGG GGACAGACGA CATCCAGGGT GAGTCGCCAG CTCCGTGTGG GACAGAGGAG 
CCTGCCCGCA CCTGCCCCAA CGGGACCAAA TGTCAGCCGT ACTGGGAAGG GCCCAACAAC 
GGCATCACTC AGTTCGACAA CATCCTGTTT GCTGTGCTCA CTGTTTTCCA GTGCATCACC 
ATGGAAGGCT GGACTGATCT CCTCTACAAT AGCAACGATG CCTCAGGGAA CACTTGGAAC 
TGGTTGTACT TCATCCCCCT CATCATCATC GGCTCCTTTT TTATGCTGAA CCTTGTGCTG 
GGTGTGCTGT CTGGGGAGTT TGCCAAAGAA AGGGAACGTG TAGAGAACCG AAGGGCTTTT 
CTGAAGCTCA GAAGACAACA GCAGATTGAA CGTGAGCTCA ATGGATACAT GGAGTGGATC 
TCGAAAGCAG AAGAGGTGAT CCTCGCGGAG GACGAGACAG ACGTGGAGCA GAGGCACCCT 
TTTGATGGAG CTCTTCGGAG AGCTACTCTG AAGAAAAGCA AGACGGACCT GCTCAACCCT 
GAGGAGGCGG AGGACCAGCT TGCTGACATC GCGTCTGTGG GGTCTCCCTT CGCCAGAGCC 
AGCATCAAAA GTGCCAAGCT GGAGAATTCG ACTTTTTTCC ACAAAAAGGA GAGAAGAATG 
CGTTTCTACA TCCGCCGCAT GGTCAAAACT CAGGCCTTCT ACTGGACCGT GCTCAGTCTG 
GTAGCCCTCA ACACGCTGTG CGTCGCCATT GTCCACTACA ACCAGCCCGA GTGGCTCTCC 
GACTTCCTCT ACTATGCAGA ATTCATTTTC TTAGGACTCT TTATGTCCGA AATGTTTATA 
AAAATGTATG GGCTCGGGAC ACGGCCTTAC TTCCACTCTT CCTTCAACTG CTTTGACTGT 
GGGGTCATCA TCGGGAGCAT CTTTGAAGTC ATCTGGGCCG TCATCAAACC GGGTACATCC 
TTTGGAATCA GCGTGTTACG AGCTCTCAGG TTACTGCGTA TTTTCAAAGT CACAAAGTAC 
TGGGCATCTC TCAGAAACCT GGTTGTCTCC CTCCTCAACT CCATGAAATC CATCATAAGT 
CTGCTGTTCC TCCTCTTCCT CTTCATTGTC GTCTTTGCCC TCTTGGGGAT GCAGCTGTTT 
GGTGGCCAGT TTAATTTTGA CGAGGGGACT CCTCCCACCA ACTTCGACAC TTTTCCAGCA 
GCAATAATGA CTGTGTTTCA GATCCTGACT GGCGAGGATT GGAATGAGGT CATGTATGAT 
GGGATCAAGT CTCAGGGGGG CGTGCAGGGC GGCATGGTGT TCTCCATCTA CTTCATCGTC 
CTCACCCTCT TCGGGAACTA CACCCTGCTG AACGTGTTCT TGGCTATCGC GGTGGACAAC 
CTGGCCAACG CCCAGGAACT CACCAAGGAT GAACAAGAAG AGGAAGAGGC AGCCAATCAG 
AAACTGGCTC TACAGAAAGC CAAGGAGGTG GCAGAAGTGA GTCCCCTGTC TGCAGCCAAC 
ATGTCCATAG CTGTGAAGGA ACAGCAGAAG AACCAGAAGC CTGCCAAGTC GGTGTGGGAG 
CAGCGCACCA GCGAGATGCG CAAGCAGAAC CTGCTGGCTA GCCGCGAGGC GCTGTACGGG 
GACGCGGCTG AGCGCTGGCC CACCACTTAC GCGCGCCCGC TGCGGCCGGA CGTGAAGACG 
CACTTGGACC GGCCGCTCGT GGTGGACCCG CAGGAGAACC GTAACAACAA CACCAACAAG 
AGCCGTGCGC CAGAAGCGCT GCGCCAAACC GCGCGGCCCC GCGAGAGCGC GCGCGACCCC 
GACGCGCGGC GCGCCTGGCC CAGCAGCCCT GAGCGCGCCC CTGGACGAGA GGGCCCGTAT 
GGCCGCGAGA GCGAGCCGCA ACAGCGCGAG CACGCGCCAC CCCGCGAGCA CGTACCCTGG 
GACGCGGATC CTGAGCGCGC CAAGGCCGGG GACGCGCCCC GCCGCCACAC GCACCGGCCT 
GTGGCCGAGG GCGAGCCTCG TCGCCACCGC GCGCGCCGCC GGCCCGGGGA CGAACCGGAC 
GACAGACCGG AGCGCAGGCC GCGTCCCCGC GACGCCACTA GGCCGGCCCG CGCTGCAGAC 
GGCGAAGGCG ATGATGGGGA GCGCAAGCGG CGACACCGAC ACGGGCCGCC GGCCCACGAT 
GACAGGGAGC GCAGACACCG GCGCAGAAAA GAGAGCCAGG GCTCTGGGGT CCCCATGTCT 
GGTCCCAACC TGTCCACCAC CAGGCCAATC CAGCAGGATC TGGGCCGCCA GGACCTGCCA 
CTGGCTGAGG ACCTGGACAA CATGAAGAAC AACAAGTTGG CCACCGGGGA GCCTGCCAGT 
CCCCACGACA GCCTGGGCCA CAGTGGCCTT CCCCCTAGCC CTGCCAAGAT CGGGAACAGC 
ACCAACCCTG GTCCCGCCTT GGCCACCAAT CCCCAGAATG CTGCCAGCCG CAGGACGCCC 
AACAACCCGG GCAACCCGTC CAACCCCGGC CCCCCCAAGA CTCCCGAGAA CAGCCTTATC 
GTCACCAACC CCAGCAGCAC CCAGCCCAAC TCAGCAAAGA CTGCCAGGAA ACCCGAGCAC 
ATGGCGGTGG AGATCCCCCC GGCCTGCCCG CCCCTCAACC ACACTGTGGT CCAAGTAAAC 
AAAAACGCCA ACCCAGACCC ACTGCCAAAG AAAGAGGAAG AGAAGAAGGA GGAAGAGGAG 
GCAGACCCCG GGGAGGATGG CCCAAAGCCC ATGCCGCCCT ACAGCTCCAT GTTCATCCTC 
TCCACCACCA ACCCCCTTCG CCGGCTGTGC CATTACATCC TGAACCTGCG CTACTTCGAG 
ATGTGCATCC TCATGGTCAT TGCCATGAGT AGCATCGCGC TGGCCGCCGA GGACCCGGTG 
CAGCCCAACG CACCCCGCAA CAACGTGCTG CGATATTTTG ACTATGTTTT CACAGGAGTG 
TTTACCTTTG AGATGGTGAT CAAGATGATC GACCTGGGCC TCGTCCTGCA TCAGGGGGCC 
TATTTCCGTG ACCTGTGGAA CATTCTGGAC TTCATAGTGG TCAGTGGGGC CCTGGTGGCC 
TTTGCCTTCA CGAGCAGTAC ACGTGGCAAT AGCAAAGGAA AGGACATCAA CACCATCAAG 
TCCCTCCGAG TCCTCCGGGT GCTACGACCT CTAAAGACCA TCAAGCGGCT GCCTAAGTTG 
AAGGCCGTAT TTGACTGCGT GGTGAACTCG CTCAAGAACG TCTTCAACAT CCTCATTGTC 
TACATGCTCT TCATGTTCAT CTTCGCCGTG GTGGCCGTGC AGCTCTTCAA GGGCAAATTC 
TTCCACTGCA CGGACGAGTC CAAGGAGTTT GAGAGAGACT GTCGAGGCAA ATACCTCCTT 
TACGAGAAGA ACGAGGTAAA GGCGCGGGAC CGCGAGTGGA AGAAATACGA CTTCCACTAC 
GACAACGTGC TCTGGGCCCT GCTCACGCTC TTTACGGTGT CCACGGGAGA GGGCTGGCCA 
CAGGTCCTCA AGCACTCAGT GGATGCCACT TTTGAGAACC AGGGCCCCAG CCCCGGGTAC 
CGCATGGAAA TGTCCATCTT CTACGTGGTC TACTTTGTGG TGTTTCCCTT CTTCTTTGTC 
AATATCTTTG TGGCCTTGAT CATCATCACC TTCCAGGAGC AGGGAGACAA GATGATGGAA 
GAATACAGCC TAGAGAAAAA TGAGAGGGCC TGCATCGACT TTGCCATCAG TGCCAAGCCG 
CTGACCAGGC ACATGCCCCA GAACAAGCAG AGCTTCCAGT ATCGAATGTG GCAGTTCGTG 
GTGTCCCCAC CCTTTGAGTA CACCATCATG GCCATGATCG CTCTCAACAC CATCGTGCTA 
ATGATGAAGT TCTATGGAGC CTCTGTGGCC TATGAAAACG CCCTTCGAGT GTTCAACATT 
GTCTTCACCT CCCTCTTCTC TCTCGAATGT GTGCTCAAAG TCATGGCTTT TGGGATTCTG 
AATTATTTCC GCGATGCCTG GAACATCTTC GACTTTGTGA CTGTTCTGGG CAGCATCACA 
GACATCCTCG TCACCGAGTT TGGGAATCCG AATAACTTCA TCAACCTGAG CTTTCTCCGC 
CTCTTCCGTG CTGCCCGACT CATCAAACTC CTCCGCCAGG GTTACACCAT CCGCATTCTC 
CTCTGGACTT TCGTGCAGTC TTTCAAGGCC CTACCTTATG TCTGTCTGCT GATCGCCATG 
CTCTTCTTCA TCTATGCCAT CATCGGGATG CAGGTGATCC CGCACCCTTG GGGTACCGGG 
GCTGCGGGAG GGAGCTTGTG TCAAAGGGAG CCAAGGTGGC TGTGGCTGCT CCCATCGTCA 
CCCCCCCCCC CAACTCTCCA GGTGTTTGGC AACATCGGCA TTGATGGGGA AGATGAGGAC 
AGCGATGAGG ATGAGTTCCA AATCACGGAG CACAATAACT TCCGGACCTT CTTCCAAGCT 
CTCATGCTTC TCTTCCGGAG CGCCACAGGG GAAGCGTGGC ACAACATCAT GCTGTCCTGC 
CTCAGCGGGA AGCCATGCGA CAAGAACTCC GGGATCCAAA AACCAGAGTG TGGCAACGAG 
TTCGCCTATT TTTACTTTGT CTCGTTCATC TTCCTTTGCT CATTTCTGAT GCTGAATCTC 
TTTGTTGCTG TCATCATGGA CAACTTCGAG TACCTCACCC GAGATTCCTC CATCCTGGGC 
CCCCACCACC TGGATGAGTA CGTGCGTGTC TGGGCAGAGT ATGACCCTGC TGCCTGCGGC 
CGCATTCACT ATAAGGACAT GTACAGTTTA TTGCGAGTAA TATCGCCCCC TCTCGGCTTA 
GGCAAGAAAT GTCCTCATAG GGTTGCTTGC AAGAGGCTCT TGCGGATGGA CCTACCCGTA 
GCGGATGACA ACACCGTTCA CTTCAACTCC ACCTTGATGG CTCTGATCCG AACCGCCCTG 
GATATCAAAA TCGCAAAGGG TGGAGCTGAC AAGCAGCAAA TGGACGCAGA GCTCCGCAAG 
GAAATGATGG CCATTTGGCC CAACCTGTCT CAGAAGACCT TGGATCTGCT GGTCACACCT 
CACAAGTCCA CGGACCTGAC AGTGGGTAAG ATCTACGCAG CCATGATGAT CATGGAGTAC 
TACCGGCAGA GCAAGGCCAA GAAGCTGCAG GCCATGCGAG AGGAGCAGAA CCGGACACCA 
CTCATGTTCC AGCGCATGGA GCCTCCATCG CCAACACAGG AGGGAGGACC CAGCCAAAAC 
GCCCTTCCCT CCACTCAGCT GGACCCCGGA GGAGGCCTGA TGGCTCAAGA AAGCAGCATG 
AAGGAGAGCC CGTCCTGGGT GACCCAGCGG GCACAGGAGA TGTTCCAGAA GACTGGTACC 
TGGAGCCCAG AGCGAGGGCC ACCCATCGAC ATGCCTAACA GCCAGCCCAA CTCCCAGTCT 
GTGGAGATGA GAGAAATGGG AACTGATGGC TACTCAGACA GCGAACACTA CCTCCCCATG 
GAAGGACAGA CCAGGGCCGC CTCCATGCCC CGCCTCCCAG CAGAGAACCA GAGGAGAAGG 
GGCCGGCCAC GTGGAAATAA CCTCAGTACC ATCTCTGATA CCAGCCCCAT GAAGCGCTCA 
GCCTCCGTGC TGGGACCCAA AGCCCGGCGA CTGGATGACT ACTCACTAGA GCGGGTACCA 
CCTGAGGAGA ACCAAAGGTA CCACCAACGC CGCCGGGACC GTGGCCACCG CACCTCTGAG 
CGCTCTCTGG GCCGATACAC TGATGTGGAC ACAGGCCTGG GGACAGATCT GAGCATGACC 
ACCCAATCGG GTGACCTGCC CTCCAAAGAT CGGGACCAGG ACCGGGGCCG GCCCAAGGAC 
CGGAAGCATC GGCCACACCA CCACCACCAC CATCATCACC ATCATCCCCC GGCCCCGGAC 
CGGGAGCGCT ACGCACAGGA GCGGCCGGAC ACCGGCCGGG CGCGGGCCCG GGAGCAGCGC 
TGGTCCCGCT CGCCCAGCGA GGGTCGGGAG CACGCGACAC ACAGACAGGG CAGTAGTTCC 
GTCAGTGGAA GCCCAGCCCC CTCTACGTCC GGCACCAGCA CGCCGCGGCG CGGCCGCCGC 
CAGCTCCCCC AGACCCCCTG CACCCCGCGG CCGCTTGTGT CCTACTCGCC CGCTCCGCGC 
AGGCCTGCGG CGCGCAGGAT GGCGGGTCCC CCAGCGCCCC CTGGCGGCTC GCCGCGGGGT 
TGCCGCCGTG CGCCGCGCTG GCCTGCGCAT GCGCCTGAGG GCCCGCGGCC GCGCGGTGCC 
GACTACACCG AGCCCGACAG CCCGCGCGAG CCGCCAGGGG GCGCGCATGA GCCCGCGCCG 
CGGTCGCCCA GGACTCCGCG CGCCGCGGGC TGCGCGTCAC CGCGCCACGG CCGCAGGCTG 
CCCAATGGTT ACTACGCGGG GCACGGCGCG CCACGGCCGC GCACAGCCCG CAGGGGCGCG 
CACGACGCGT ACAGCGAGAG CGAGGACGAC TGGTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017456666.1
CDS	107..7645
Translation	MVSSPGISERGPWMPRASWVSDPMRSPLNHELLHSSRYPGTDCTLAGSSRTLHVPWSEQMSGSLEGPTLKRRGGVVDHRDVILAHQAHKMHSTPQARRKEWEMARFGDEMPGRYGAGGGGSGPAAGVVVGAAGGRGAGGSRQGGQPGAQRMYKQSMAQRARTMALYNPIPVRQNCLTVNRSLFLFSEDNVVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDDDKTPMSERLDDTEPYFIGIFCFEAGIKIVALGFAFHKGSYLRNGWNVMDFVVVLTGILATVGTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILIFAIIGLEFYMGKFHTTCFEEGTDDIQGESPAPCGTEEPARTCPNGTKCQPYWEGPNNGITQFDNILFAVLTVFQCITMEGWTDLLYNSNDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYMEWISKAEEVILAEDETDVEQRHPFDGALRRATLKKSKTDLLNPEEAEDQLADIASVGSPFARASIKSAKLENSTFFHKKERRMRFYIRRMVKTQAFYWTVLSLVALNTLCVAIVHYNQPEWLSDFLYYAEFIFLGLFMSEMFIKMYGLGTRPYFHSSFNCFDCGVIIGSIFEVIWAVIKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFDEGTPPTNFDTFPAAIMTVFQILTGEDWNEVMYDGIKSQGGVQGGMVFSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAANQKLALQKAKEVAEVSPLSAANMSIAVKEQQKNQKPAKSVWEQRTSEMRKQNLLASREALYGDAAERWPTTYARPLRPDVKTHLDRPLVVDPQENRNNNTNKSRAPEALRQTARPRESARDPDARRAWPSSPERAPGREGPYGRESEPQQREHAPPREHVPWDADPERAKAGDAPRRHTHRPVAEGEPRRHRARRRPGDEPDDRPERRPRPRDATRPARAADGEGDDGERKRRHRHGPPAHDDRERRHRRRKESQGSGVPMSGPNLSTTRPIQQDLGRQDLPLAEDLDNMKNNKLATGEPASPHDSLGHSGLPPSPAKIGNSTNPGPALATNPQNAASRRTPNNPGNPSNPGPPKTPENSLIVTNPSSTQPNSAKTARKPEHMAVEIPPACPPLNHTVVQVNKNANPDPLPKKEEEKKEEEEADPGEDGPKPMPPYSSMFILSTTNPLRRLCHYILNLRYFEMCILMVIAMSSIALAAEDPVQPNAPRNNVLRYFDYVFTGVFTFEMVIKMIDLGLVLHQGAYFRDLWNILDFIVVSGALVAFAFTSSTRGNSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYMLFMFIFAVVAVQLFKGKFFHCTDESKEFERDCRGKYLLYEKNEVKARDREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATFENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEEYSLEKNERACIDFAISAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYTIMAMIALNTIVLMMKFYGASVAYENALRVFNIVFTSLFSLECVLKVMAFGILNYFRDAWNIFDFVTVLGSITDILVTEFGNPNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVIPHPWGTGAAGGSLCQREPRWLWLLPSSPPPPTLQVFGNIGIDGEDEDSDEDEFQITEHNNFRTFFQALMLLFRSATGEAWHNIMLSCLSGKPCDKNSGIQKPECGNEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRVWAEYDPAACGRIHYKDMYSLLRVISPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKQQMDAELRKEMMAIWPNLSQKTLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKKLQAMREEQNRTPLMFQRMEPPSPTQEGGPSQNALPSTQLDPGGGLMAQESSMKESPSWVTQRAQEMFQKTGTWSPERGPPIDMPNSQPNSQSVEMREMGTDGYSDSEHYLPMEGQTRAASMPRLPAENQRRRGRPRGNNLSTISDTSPMKRSASVLGPKARRLDDYSLERVPPEENQRYHQRRRDRGHRTSERSLGRYTDVDTGLGTDLSMTTQSGDLPSKDRDQDRGRPKDRKHRPHHHHHHHHHHPPAPDRERYAQERPDTGRARAREQRWSRSPSEGREHATHRQGSSSVSGSPAPSTSGTSTPRRGRRQLPQTPCTPRPLVSYSPAPRRPAARRMAGPPAPPGGSPRGCRRAPRWPAHAPEGPRPRGADYTEPDSPREPPGGAHEPAPRSPRTPRAAGCASPRHGRRLPNGYYAGHGAPRPRTARRGAHDAYSESEDDWC

Target ORF information:

RefSeq Version	XM_017601177.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel subunit alpha1 A (Cacna1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017601177.1

ORF Insert Sequence:

ATGGTTTCCT CTCCTGGGAT TTCTGAGCGA GGCCCATGGA TGCCGAGGGC CTCCTGGGTC 
TCTGACCCCA TGCGTTCTCC CCTCAATCAT GAGCTCCTGC ACAGCTCGAG GTACCCAGGC 
ACGGACTGCA CCCTGGCAGG TTCTTCCAGG ACTTTGCATG TCCCGTGGTC CGAGCAGATG 
AGCGGAAGCC TCGAGGGGCC AACTCTGAAA CGGCGTGGTG GCGTGGTGGA CCACCGGGAT 
GTCATCTTGG CCCACCAGGC GCACAAAATG CACAGCACCC CTCAGGCCAG GAGGAAAGAG 
TGGGAAATGG CCCGCTTTGG AGACGAGATG CCGGGCCGCT ACGGCGCAGG CGGAGGAGGC 
TCAGGGCCGG CCGCCGGGGT GGTCGTGGGC GCCGCGGGCG GCCGAGGAGC CGGGGGCAGC 
CGGCAGGGCG GGCAGCCCGG AGCGCAGAGG ATGTACAAGC AGTCGATGGC GCAGAGAGCG 
CGGACCATGG CCCTCTACAA CCCCATCCCT GTCCGCCAGA ACTGCCTCAC GGTCAACCGC 
TCCCTGTTCC TCTTCAGTGA AGACAACGTG GTGAGAAAAT ACGCCAAAAA GATCACGGAA 
TGGCCTCCCT TCGAGTACAT GATCCTGGCC ACCATCATTG CTAACTGCAT CGTCCTGGCC 
CTGGAGCAGC ACCTCCCTGA TGATGACAAG ACACCCATGT CCGAGCGGCT GGATGACACA 
GAACCCTATT TCATTGGCAT CTTCTGTTTT GAGGCTGGAA TTAAGATCGT GGCTCTTGGC 
TTTGCCTTCC ACAAAGGCTC CTACCTGAGG AATGGCTGGA ACGTCATGGA CTTTGTCGTG 
GTGCTAACAG GCATCTTGGC CACTGTCGGG ACGGAGTTTG ATCTACGGAC ACTGAGGGCG 
GTTCGTGTGC TGCGGCCACT CAAGCTGGTG TCTGGAATCC CAAGTTTACA AGTCGTCCTG 
AAGTCAATCA TGAAGGCGAT GATCCCTCTG CTGCAGATCG GCCTCCTCCT GTTTTTTGCA 
ATCCTTATTT TTGCAATCAT AGGGTTAGAA TTTTATATGG GAAAATTTCA TACCACCTGC 
TTTGAAGAGG GGACAGACGA CATCCAGGGT GAGTCGCCAG CTCCGTGTGG GACAGAGGAG 
CCTGCCCGCA CCTGCCCCAA CGGGACCAAA TGTCAGCCGT ACTGGGAAGG GCCCAACAAC 
GGCATCACTC AGTTCGACAA CATCCTGTTT GCTGTGCTCA CTGTTTTCCA GTGCATCACC 
ATGGAAGGCT GGACTGATCT CCTCTACAAT AGCAACGATG CCTCAGGGAA CACTTGGAAC 
TGGTTGTACT TCATCCCCCT CATCATCATC GGCTCCTTTT TTATGCTGAA CCTTGTGCTG 
GGTGTGCTGT CTGGGGAGTT TGCCAAAGAA AGGGAACGTG TAGAGAACCG AAGGGCTTTT 
CTGAAGCTCA GAAGACAACA GCAGATTGAA CGTGAGCTCA ATGGATACAT GGAGTGGATC 
TCGAAAGCAG AAGAGGTGAT CCTCGCGGAG GACGAGACAG ACGTGGAGCA GAGGCACCCT 
TTTGATGGAG CTCTTCGGAG AGCTACTCTG AAGAAAAGCA AGACGGACCT GCTCAACCCT 
GAGGAGGCGG AGGACCAGCT TGCTGACATC GCGTCTGTGG GGTCTCCCTT CGCCAGAGCC 
AGCATCAAAA GTGCCAAGCT GGAGAATTCG ACTTTTTTCC ACAAAAAGGA GAGAAGAATG 
CGTTTCTACA TCCGCCGCAT GGTCAAAACT CAGGCCTTCT ACTGGACCGT GCTCAGTCTG 
GTAGCCCTCA ACACGCTGTG CGTCGCCATT GTCCACTACA ACCAGCCCGA GTGGCTCTCC 
GACTTCCTCT ACTATGCAGA ATTCATTTTC TTAGGACTCT TTATGTCCGA AATGTTTATA 
AAAATGTATG GGCTCGGGAC ACGGCCTTAC TTCCACTCTT CCTTCAACTG CTTTGACTGT 
GGGGTCATCA TCGGGAGCAT CTTTGAAGTC ATCTGGGCCG TCATCAAACC GGGTACATCC 
TTTGGAATCA GCGTGTTACG AGCTCTCAGG TTACTGCGTA TTTTCAAAGT CACAAAGTAC 
TGGGCATCTC TCAGAAACCT GGTTGTCTCC CTCCTCAACT CCATGAAATC CATCATAAGT 
CTGCTGTTCC TCCTCTTCCT CTTCATTGTC GTCTTTGCCC TCTTGGGGAT GCAGCTGTTT 
GGTGGCCAGT TTAATTTTGA CGAGGGGACT CCTCCCACCA ACTTCGACAC TTTTCCAGCA 
GCAATAATGA CTGTGTTTCA GATCCTGACT GGCGAGGATT GGAATGAGGT CATGTATGAT 
GGGATCAAGT CTCAGGGGGG CGTGCAGGGC GGCATGGTGT TCTCCATCTA CTTCATCGTC 
CTCACCCTCT TCGGGAACTA CACCCTGCTG AACGTGTTCT TGGCTATCGC GGTGGACAAC 
CTGGCCAACG CCCAGGAACT CACCAAGGAT GAACAAGAAG AGGAAGAGGC AGCCAATCAG 
AAACTGGCTC TACAGAAAGC CAAGGAGGTG GCAGAAGTGA GTCCCCTGTC TGCAGCCAAC 
ATGTCCATAG CTGTGAAGGA ACAGCAGAAG AACCAGAAGC CTGCCAAGTC GGTGTGGGAG 
CAGCGCACCA GCGAGATGCG CAAGCAGAAC CTGCTGGCTA GCCGCGAGGC GCTGTACGGG 
GACGCGGCTG AGCGCTGGCC CACCACTTAC GCGCGCCCGC TGCGGCCGGA CGTGAAGACG 
CACTTGGACC GGCCGCTCGT GGTGGACCCG CAGGAGAACC GTAACAACAA CACCAACAAG 
AGCCGTGCGC CAGAAGCGCT GCGCCAAACC GCGCGGCCCC GCGAGAGCGC GCGCGACCCC 
GACGCGCGGC GCGCCTGGCC CAGCAGCCCT GAGCGCGCCC CTGGACGAGA GGGCCCGTAT 
GGCCGCGAGA GCGAGCCGCA ACAGCGCGAG CACGCGCCAC CCCGCGAGCA CGTACCCTGG 
GACGCGGATC CTGAGCGCGC CAAGGCCGGG GACGCGCCCC GCCGCCACAC GCACCGGCCT 
GTGGCCGAGG GCGAGCCTCG TCGCCACCGC GCGCGCCGCC GGCCCGGGGA CGAACCGGAC 
GACAGACCGG AGCGCAGGCC GCGTCCCCGC GACGCCACTA GGCCGGCCCG CGCTGCAGAC 
GGCGAAGGCG ATGATGGGGA GCGCAAGCGG CGACACCGAC ACGGGCCGCC GGCCCACGAT 
GACAGGGAGC GCAGACACCG GCGCAGAAAA GAGAGCCAGG GCTCTGGGGT CCCCATGTCT 
GGTCCCAACC TGTCCACCAC CAGGCCAATC CAGCAGGATC TGGGCCGCCA GGACCTGCCA 
CTGGCTGAGG ACCTGGACAA CATGAAGAAC AACAAGTTGG CCACCGGGGA GCCTGCCAGT 
CCCCACGACA GCCTGGGCCA CAGTGGCCTT CCCCCTAGCC CTGCCAAGAT CGGGAACAGC 
ACCAACCCTG GTCCCGCCTT GGCCACCAAT CCCCAGAATG CTGCCAGCCG CAGGACGCCC 
AACAACCCGG GCAACCCGTC CAACCCCGGC CCCCCCAAGA CTCCCGAGAA CAGCCTTATC 
GTCACCAACC CCAGCAGCAC CCAGCCCAAC TCAGCAAAGA CTGCCAGGAA ACCCGAGCAC 
ATGGCGGTGG AGATCCCCCC GGCCTGCCCG CCCCTCAACC ACACTGTGGT CCAAGTAAAC 
AAAAACGCCA ACCCAGACCC ACTGCCAAAG AAAGAGGAAG AGAAGAAGGA GGAAGAGGAG 
GCAGACCCCG GGGAGGATGG CCCAAAGCCC ATGCCGCCCT ACAGCTCCAT GTTCATCCTC 
TCCACCACCA ACCCCCTTCG CCGGCTGTGC CATTACATCC TGAACCTGCG CTACTTCGAG 
ATGTGCATCC TCATGGTCAT TGCCATGAGT AGCATCGCGC TGGCCGCCGA GGACCCGGTG 
CAGCCCAACG CACCCCGCAA CAACGTGCTG CGATATTTTG ACTATGTTTT CACAGGAGTG 
TTTACCTTTG AGATGGTGAT CAAGATGATC GACCTGGGCC TCGTCCTGCA TCAGGGGGCC 
TATTTCCGTG ACCTGTGGAA CATTCTGGAC TTCATAGTGG TCAGTGGGGC CCTGGTGGCC 
TTTGCCTTCA CGAGCAGTAC ACGTGGCAAT AGCAAAGGAA AGGACATCAA CACCATCAAG 
TCCCTCCGAG TCCTCCGGGT GCTACGACCT CTAAAGACCA TCAAGCGGCT GCCTAAGTTG 
AAGGCCGTAT TTGACTGCGT GGTGAACTCG CTCAAGAACG TCTTCAACAT CCTCATTGTC 
TACATGCTCT TCATGTTCAT CTTCGCCGTG GTGGCCGTGC AGCTCTTCAA GGGCAAATTC 
TTCCACTGCA CGGACGAGTC CAAGGAGTTT GAGAGAGACT GTCGAGGCAA ATACCTCCTT 
TACGAGAAGA ACGAGGTAAA GGCGCGGGAC CGCGAGTGGA AGAAATACGA CTTCCACTAC 
GACAACGTGC TCTGGGCCCT GCTCACGCTC TTTACGGTGT CCACGGGAGA GGGCTGGCCA 
CAGGTCCTCA AGCACTCAGT GGATGCCACT TTTGAGAACC AGGGCCCCAG CCCCGGGTAC 
CGCATGGAAA TGTCCATCTT CTACGTGGTC TACTTTGTGG TGTTTCCCTT CTTCTTTGTC 
AATATCTTTG TGGCCTTGAT CATCATCACC TTCCAGGAGC AGGGAGACAA GATGATGGAA 
GAATACAGCC TAGAGAAAAA TGAGAGGGCC TGCATCGACT TTGCCATCAG TGCCAAGCCG 
CTGACCAGGC ACATGCCCCA GAACAAGCAG AGCTTCCAGT ATCGAATGTG GCAGTTCGTG 
GTGTCCCCAC CCTTTGAGTA CACCATCATG GCCATGATCG CTCTCAACAC CATCGTGCTA 
ATGATGAAGT TCTATGGAGC CTCTGTGGCC TATGAAAACG CCCTTCGAGT GTTCAACATT 
GTCTTCACCT CCCTCTTCTC TCTCGAATGT GTGCTCAAAG TCATGGCTTT TGGGATTCTG 
AATTATTTCC GCGATGCCTG GAACATCTTC GACTTTGTGA CTGTTCTGGG CAGCATCACA 
GACATCCTCG TCACCGAGTT TGGGAATCCG AATAACTTCA TCAACCTGAG CTTTCTCCGC 
CTCTTCCGTG CTGCCCGACT CATCAAACTC CTCCGCCAGG GTTACACCAT CCGCATTCTC 
CTCTGGACTT TCGTGCAGTC TTTCAAGGCC CTACCTTATG TCTGTCTGCT GATCGCCATG 
CTCTTCTTCA TCTATGCCAT CATCGGGATG CAGGTGATCC CGCACCCTTG GGGTACCGGG 
GCTGCGGGAG GGAGCTTGTG TCAAAGGGAG CCAAGGTGGC TGTGGCTGCT CCCATCGTCA 
CCCCCCCCCC CAACTCTCCA GGTGTTTGGC AACATCGGCA TTGATGGGGA AGATGAGGAC 
AGCGATGAGG ATGAGTTCCA AATCACGGAG CACAATAACT TCCGGACCTT CTTCCAAGCT 
CTCATGCTTC TCTTCCGGAG CGCCACAGGG GAAGCGTGGC ACAACATCAT GCTGTCCTGC 
CTCAGCGGGA AGCCATGCGA CAAGAACTCC GGGATCCAAA AACCAGAGTG TGGCAACGAG 
TTCGCCTATT TTTACTTTGT CTCGTTCATC TTCCTTTGCT CATTTCTGAT GCTGAATCTC 
TTTGTTGCTG TCATCATGGA CAACTTCGAG TACCTCACCC GAGATTCCTC CATCCTGGGC 
CCCCACCACC TGGATGAGTA CGTGCGTGTC TGGGCAGAGT ATGACCCTGC TGCCTGCGGC 
CGCATTCACT ATAAGGACAT GTACAGTTTA TTGCGAGTAA TATCGCCCCC TCTCGGCTTA 
GGCAAGAAAT GTCCTCATAG GGTTGCTTGC AAGAGGCTCT TGCGGATGGA CCTACCCGTA 
GCGGATGACA ACACCGTTCA CTTCAACTCC ACCTTGATGG CTCTGATCCG AACCGCCCTG 
GATATCAAAA TCGCAAAGGG TGGAGCTGAC AAGCAGCAAA TGGACGCAGA GCTCCGCAAG 
GAAATGATGG CCATTTGGCC CAACCTGTCT CAGAAGACCT TGGATCTGCT GGTCACACCT 
CACAAGTCCA CGGACCTGAC AGTGGGTAAG ATCTACGCAG CCATGATGAT CATGGAGTAC 
TACCGGCAGA GCAAGGCCAA GAAGCTGCAG GCCATGCGAG AGGAGCAGAA CCGGACACCA 
CTCATGTTCC AGCGCATGGA GCCTCCATCG CCAACACAGG AGGGAGGACC CAGCCAAAAC 
GCCCTTCCCT CCACTCAGCT GGACCCCGGA GGAGGCCTGA TGGCTCAAGA AAGCAGCATG 
AAGGAGAGCC CGTCCTGGGT GACCCAGCGG GCACAGGAGA TGTTCCAGAA GACTGGTACC 
TGGAGCCCAG AGCGAGGGCC ACCCATCGAC ATGCCTAACA GCCAGCCCAA CTCCCAGTCT 
GTGGAGATGA GAGAAATGGG AACTGATGGC TACTCAGACA GCGAACACTA CCTCCCCATG 
GAAGGACAGA CCAGGGCCGC CTCCATGCCC CGCCTCCCAG CAGAGAACCA GAGGAGAAGG 
GGCCGGCCAC GTGGAAATAA CCTCAGTACC ATCTCTGATA CCAGCCCCAT GAAGCGCTCA 
GCCTCCGTGC TGGGACCCAA AGCCCGGCGA CTGGATGACT ACTCACTAGA GCGGGTACCA 
CCTGAGGAGA ACCAAAGGTA CCACCAACGC CGCCGGGACC GTGGCCACCG CACCTCTGAG 
CGCTCTCTGG GCCGATACAC TGATGTGGAC ACAGGCCTGG GGACAGATCT GAGCATGACC 
ACCCAATCGG GTGACCTGCC CTCCAAAGAT CGGGACCAGG ACCGGGGCCG GCCCAAGGAC 
CGGAAGCATC GGCCACACCA CCACCACCAC CATCATCACC ATCATCCCCC GGCCCCGGAC 
CGGGAGCGCT ACGCACAGGA GCGGCCGGAC ACCGGCCGGG CGCGGGCCCG GGAGCAGCGC 
TGGTCCCGCT CGCCCAGCGA GGGTCGGGAG CACGCGACAC ACAGACAGGG CAGTAGTTCC 
GTCAGTGGAA GCCCAGCCCC CTCTACGTCC GGCACCAGCA CGCCGCGGCG CGGCCGCCGC 
CAGCTCCCCC AGACCCCCTG CACCCCGCGG CCGCTTGTGT CCTACTCGCC CGCTCCGCGC 
AGGCCTGCGG CGCGCAGGAT GGCGGGTCCC CCAGCGCCCC CTGGCGGCTC GCCGCGGGGT 
TGCCGCCGTG CGCCGCGCTG GCCTGCGCAT GCGCCTGAGG GCCCGCGGCC GCGCGGTGCC 
GACTACACCG AGCCCGACAG CCCGCGCGAG CCGCCAGGGG GCGCGCATGA GCCCGCGCCG 
CGGTCGCCCA GGACTCCGCG CGCCGCGGGC TGCGCGTCAC CGCGCCACGG CCGCAGGCTG 
CCCAATGGTT ACTACGCGGG GCACGGCGCG CCACGGCCGC GCACAGCCCG CAGGGGCGCG 
CACGACGCGT ACAGCGAGAG CGAGGACGAC TGGTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1a ( XM_008772348.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008770570.2 Rattus norvegicus calcium voltage-gated channel subunit alpha1 A (Cacna1a), transcript variant X2, mRNA.

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CloneID	ORa59365
Clone ID Related Accession (Same CDS sequence)	XM_008772348.2
Accession Version	XM_008772348.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7539bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008772348.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTCCT CTCCTGGGAT TTCTGAGCGA GGCCCATGGA TGCCGAGGGC CTCCTGGGTC 
TCTGACCCCA TGCGTTCTCC CCTCAATCAT GAGCTCCTGC ACAGCTCGAG GTACCCAGGC 
ACGGACTGCA CCCTGGCAGG TTCTTCCAGG ACTTTGCATG TCCCGTGGTC CGAGCAGATG 
AGCGGAAGCC TCGAGGGGCC AACTCTGAAA CGGCGTGGTG GCGTGGTGGA CCACCGGGAT 
GTCATCTTGG CCCACCAGGC GCACAAAATG CACAGCACCC CTCAGGCCAG GAGGAAAGAG 
TGGGAAATGG CCCGCTTTGG AGACGAGATG CCGGGCCGCT ACGGCGCAGG CGGAGGAGGC 
TCAGGGCCGG CCGCCGGGGT GGTCGTGGGC GCCGCGGGCG GCCGAGGAGC CGGGGGCAGC 
CGGCAGGGCG GGCAGCCCGG AGCGCAGAGG ATGTACAAGC AGTCGATGGC GCAGAGAGCG 
CGGACCATGG CCCTCTACAA CCCCATCCCT GTCCGCCAGA ACTGCCTCAC GGTCAACCGC 
TCCCTGTTCC TCTTCAGTGA AGACAACGTG GTGAGAAAAT ACGCCAAAAA GATCACGGAA 
TGGCCTCCCT TCGAGTACAT GATCCTGGCC ACCATCATTG CTAACTGCAT CGTCCTGGCC 
CTGGAGCAGC ACCTCCCTGA TGATGACAAG ACACCCATGT CCGAGCGGCT GGATGACACA 
GAACCCTATT TCATTGGCAT CTTCTGTTTT GAGGCTGGAA TTAAGATCGT GGCTCTTGGC 
TTTGCCTTCC ACAAAGGCTC CTACCTGAGG AATGGCTGGA ACGTCATGGA CTTTGTCGTG 
GTGCTAACAG GCATCTTGGC CACTGTCGGG ACGGAGTTTG ATCTACGGAC ACTGAGGGCG 
GTTCGTGTGC TGCGGCCACT CAAGCTGGTG TCTGGAATCC CAAGTTTACA AGTCGTCCTG 
AAGTCAATCA TGAAGGCGAT GATCCCTCTG CTGCAGATCG GCCTCCTCCT GTTTTTTGCA 
ATCCTTATTT TTGCAATCAT AGGGTTAGAA TTTTATATGG GAAAATTTCA TACCACCTGC 
TTTGAAGAGG GGACAGACGA CATCCAGGGT GAGTCGCCAG CTCCGTGTGG GACAGAGGAG 
CCTGCCCGCA CCTGCCCCAA CGGGACCAAA TGTCAGCCGT ACTGGGAAGG GCCCAACAAC 
GGCATCACTC AGTTCGACAA CATCCTGTTT GCTGTGCTCA CTGTTTTCCA GTGCATCACC 
ATGGAAGGCT GGACTGATCT CCTCTACAAT AGCAACGATG CCTCAGGGAA CACTTGGAAC 
TGGTTGTACT TCATCCCCCT CATCATCATC GGCTCCTTTT TTATGCTGAA CCTTGTGCTG 
GGTGTGCTGT CTGGGGAGTT TGCCAAAGAA AGGGAACGTG TAGAGAACCG AAGGGCTTTT 
CTGAAGCTCA GAAGACAACA GCAGATTGAA CGTGAGCTCA ATGGATACAT GGAGTGGATC 
TCGAAAGCAG AAGAGGTGAT CCTCGCGGAG GACGAGACAG ACGTGGAGCA GAGGCACCCT 
TTTGATGGAG CTCTTCGGAG AGCTACTCTG AAGAAAAGCA AGACGGACCT GCTCAACCCT 
GAGGAGGCGG AGGACCAGCT TGCTGACATC GCGTCTGTGG GGTCTCCCTT CGCCAGAGCC 
AGCATCAAAA GTGCCAAGCT GGAGAATTCG ACTTTTTTCC ACAAAAAGGA GAGAAGAATG 
CGTTTCTACA TCCGCCGCAT GGTCAAAACT CAGGCCTTCT ACTGGACCGT GCTCAGTCTG 
GTAGCCCTCA ACACGCTGTG CGTCGCCATT GTCCACTACA ACCAGCCCGA GTGGCTCTCC 
GACTTCCTCT ACTATGCAGA ATTCATTTTC TTAGGACTCT TTATGTCCGA AATGTTTATA 
AAAATGTATG GGCTCGGGAC ACGGCCTTAC TTCCACTCTT CCTTCAACTG CTTTGACTGT 
GGGGTCATCA TCGGGAGCAT CTTTGAAGTC ATCTGGGCCG TCATCAAACC GGGTACATCC 
TTTGGAATCA GCGTGTTACG AGCTCTCAGG TTACTGCGTA TTTTCAAAGT CACAAAGTAC 
TGGGCATCTC TCAGAAACCT GGTTGTCTCC CTCCTCAACT CCATGAAATC CATCATAAGT 
CTGCTGTTCC TCCTCTTCCT CTTCATTGTC GTCTTTGCCC TCTTGGGGAT GCAGCTGTTT 
GGTGGCCAGT TTAATTTTGA CGAGGGGACT CCTCCCACCA ACTTCGACAC TTTTCCAGCA 
GCAATAATGA CTGTGTTTCA GATCCTGACT GGCGAGGATT GGAATGAGGT CATGTATGAT 
GGGATCAAGT CTCAGGGGGG CGTGCAGGGC GGCATGGTGT TCTCCATCTA CTTCATCGTC 
CTCACCCTCT TCGGGAACTA CACCCTGCTG AACGTGTTCT TGGCTATCGC GGTGGACAAC 
CTGGCCAACG CCCAGGAACT CACCAAGGAT GAACAAGAAG AGGAAGAGGC AGCCAATCAG 
AAACTGGCTC TACAGAAAGC CAAGGAGGTG GCAGAAGTGA GTCCCCTGTC TGCAGCCAAC 
ATGTCCATAG CTGTGAAGGA ACAGCAGAAG AACCAGAAGC CTGCCAAGTC GGTGTGGGAG 
CAGCGCACCA GCGAGATGCG CAAGCAGAAC CTGCTGGCTA GCCGCGAGGC GCTGTACGGG 
GACGCGGCTG AGCGCTGGCC CACCACTTAC GCGCGCCCGC TGCGGCCGGA CGTGAAGACG 
CACTTGGACC GGCCGCTCGT GGTGGACCCG CAGGAGAACC GTAACAACAA CACCAACAAG 
AGCCGTGCGC CAGAAGCGCT GCGCCAAACC GCGCGGCCCC GCGAGAGCGC GCGCGACCCC 
GACGCGCGGC GCGCCTGGCC CAGCAGCCCT GAGCGCGCCC CTGGACGAGA GGGCCCGTAT 
GGCCGCGAGA GCGAGCCGCA ACAGCGCGAG CACGCGCCAC CCCGCGAGCA CGTACCCTGG 
GACGCGGATC CTGAGCGCGC CAAGGCCGGG GACGCGCCCC GCCGCCACAC GCACCGGCCT 
GTGGCCGAGG GCGAGCCTCG TCGCCACCGC GCGCGCCGCC GGCCCGGGGA CGAACCGGAC 
GACAGACCGG AGCGCAGGCC GCGTCCCCGC GACGCCACTA GGCCGGCCCG CGCTGCAGAC 
GGCGAAGGCG ATGATGGGGA GCGCAAGCGG CGACACCGAC ACGGGCCGCC GGCCCACGAT 
GACAGGGAGC GCAGACACCG GCGCAGAAAA GAGAGCCAGG GCTCTGGGGT CCCCATGTCT 
GGTCCCAACC TGTCCACCAC CAGGCCAATC CAGCAGGATC TGGGCCGCCA GGACCTGCCA 
CTGGCTGAGG ACCTGGACAA CATGAAGAAC AACAAGTTGG CCACCGGGGA GCCTGCCAGT 
CCCCACGACA GCCTGGGCCA CAGTGGCCTT CCCCCTAGCC CTGCCAAGAT CGGGAACAGC 
ACCAACCCTG GTCCCGCCTT GGCCACCAAT CCCCAGAATG CTGCCAGCCG CAGGACGCCC 
AACAACCCGG GCAACCCGTC CAACCCCGGC CCCCCCAAGA CTCCCGAGAA CAGCCTTATC 
GTCACCAACC CCAGCAGCAC CCAGCCCAAC TCAGCAAAGA CTGCCAGGAA ACCCGAGCAC 
ATGGCGGTGG AGATCCCCCC GGCCTGCCCG CCCCTCAACC ACACTGTGGT CCAAGTAAAC 
AAAAACGCCA ACCCAGACCC ACTGCCAAAG AAAGAGGAAG AGAAGAAGGA GGAAGAGGAG 
GCAGACCCCG GGGAGGATGG CCCAAAGCCC ATGCCGCCCT ACAGCTCCAT GTTCATCCTC 
TCCACCACCA ACCCCCTTCG CCGGCTGTGC CATTACATCC TGAACCTGCG CTACTTCGAG 
ATGTGCATCC TCATGGTCAT TGCCATGAGT AGCATCGCGC TGGCCGCCGA GGACCCGGTG 
CAGCCCAACG CACCCCGCAA CAACGTGCTG CGATATTTTG ACTATGTTTT CACAGGAGTG 
TTTACCTTTG AGATGGTGAT CAAGATGATC GACCTGGGCC TCGTCCTGCA TCAGGGGGCC 
TATTTCCGTG ACCTGTGGAA CATTCTGGAC TTCATAGTGG TCAGTGGGGC CCTGGTGGCC 
TTTGCCTTCA CGAGCAGTAC ACGTGGCAAT AGCAAAGGAA AGGACATCAA CACCATCAAG 
TCCCTCCGAG TCCTCCGGGT GCTACGACCT CTAAAGACCA TCAAGCGGCT GCCTAAGTTG 
AAGGCCGTAT TTGACTGCGT GGTGAACTCG CTCAAGAACG TCTTCAACAT CCTCATTGTC 
TACATGCTCT TCATGTTCAT CTTCGCCGTG GTGGCCGTGC AGCTCTTCAA GGGCAAATTC 
TTCCACTGCA CGGACGAGTC CAAGGAGTTT GAGAGAGACT GTCGAGGCAA ATACCTCCTT 
TACGAGAAGA ACGAGGTAAA GGCGCGGGAC CGCGAGTGGA AGAAATACGA CTTCCACTAC 
GACAACGTGC TCTGGGCCCT GCTCACGCTC TTTACGGTGT CCACGGGAGA GGGCTGGCCA 
CAGGTCCTCA AGCACTCAGT GGATGCCACT TTTGAGAACC AGGGCCCCAG CCCCGGGTAC 
CGCATGGAAA TGTCCATCTT CTACGTGGTC TACTTTGTGG TGTTTCCCTT CTTCTTTGTC 
AATATCTTTG TGGCCTTGAT CATCATCACC TTCCAGGAGC AGGGAGACAA GATGATGGAA 
GAATACAGCC TAGAGAAAAA TGAGAGGGCC TGCATCGACT TTGCCATCAG TGCCAAGCCG 
CTGACCAGGC ACATGCCCCA GAACAAGCAG AGCTTCCAGT ATCGAATGTG GCAGTTCGTG 
GTGTCCCCAC CCTTTGAGTA CACCATCATG GCCATGATCG CTCTCAACAC CATCGTGCTA 
ATGATGAAGT TCTATGGAGC CTCTGTGGCC TATGAAAACG CCCTTCGAGT GTTCAACATT 
GTCTTCACCT CCCTCTTCTC TCTCGAATGT GTGCTCAAAG TCATGGCTTT TGGGATTCTG 
AATTATTTCC GCGATGCCTG GAACATCTTC GACTTTGTGA CTGTTCTGGG CAGCATCACA 
GACATCCTCG TCACCGAGTT TGGGAATCCG AATAACTTCA TCAACCTGAG CTTTCTCCGC 
CTCTTCCGTG CTGCCCGACT CATCAAACTC CTCCGCCAGG GTTACACCAT CCGCATTCTC 
CTCTGGACTT TCGTGCAGTC TTTCAAGGCC CTACCTTATG TCTGTCTGCT GATCGCCATG 
CTCTTCTTCA TCTATGCCAT CATCGGGATG CAGGTGATCC CGCACCCTTG GGGTACCGGG 
GCTGCGGGAG GGAGCTTGTG TCAAAGGGAG CCAAGGTGGC TGTGGCTGCT CCCATCGTCA 
CCCCCCCCCC CAACTCTCCA GGTGTTTGGC AACATCGGCA TTGATGGGGA AGATGAGGAC 
AGCGATGAGG ATGAGTTCCA AATCACGGAG CACAATAACT TCCGGACCTT CTTCCAAGCT 
CTCATGCTTC TCTTCCGGAG CGCCACAGGG GAAGCGTGGC ACAACATCAT GCTGTCCTGC 
CTCAGCGGGA AGCCATGCGA CAAGAACTCC GGGATCCAAA AACCAGAGTG TGGCAACGAG 
TTCGCCTATT TTTACTTTGT CTCGTTCATC TTCCTTTGCT CATTTCTGAT GCTGAATCTC 
TTTGTTGCTG TCATCATGGA CAACTTCGAG TACCTCACCC GAGATTCCTC CATCCTGGGC 
CCCCACCACC TGGATGAGTA CGTGCGTGTC TGGGCAGAGT ATGACCCTGC TGCCTGGGGT 
CGCATGCCTT ACCCGGACAT GTATCAGATG CTGAGACACA TGTCCCCGCC CCTGGGTCTT 
GGGAAGAAGT GCCCGGCCAG AGTGGCTTAC AAGAGGCTCT TGCGGATGGA CCTACCCGTA 
GCGGATGACA ACACCGTTCA CTTCAACTCC ACCTTGATGG CTCTGATCCG AACCGCCCTG 
GATATCAAAA TCGCAAAGGG TGGAGCTGAC AAGCAGCAAA TGGACGCAGA GCTCCGCAAG 
GAAATGATGG CCATTTGGCC CAACCTGTCT CAGAAGACCT TGGATCTGCT GGTCACACCT 
CACAAGTCCA CGGACCTGAC AGTGGGTAAG ATCTACGCAG CCATGATGAT CATGGAGTAC 
TACCGGCAGA GCAAGGCCAA GAAGCTGCAG GCCATGCGAG AGGAGCAGAA CCGGACACCA 
CTCATGTTCC AGCGCATGGA GCCTCCATCG CCAACACAGG AGGGAGGACC CAGCCAAAAC 
GCCCTTCCCT CCACTCAGCT GGACCCCGGA GGAGGCCTGA TGGCTCAAGA AAGCAGCATG 
AAGGAGAGCC CGTCCTGGGT GACCCAGCGG GCACAGGAGA TGTTCCAGAA GACTGGTACC 
TGGAGCCCAG AGCGAGGGCC ACCCATCGAC ATGCCTAACA GCCAGCCCAA CTCCCAGTCT 
GTGGAGATGA GAGAAATGGG AACTGATGGC TACTCAGACA GCGAACACTA CCTCCCCATG 
GAAGGACAGA CCAGGGCCGC CTCCATGCCC CGCCTCCCAG CAGAGAACCA GAGGAGAAGG 
GGCCGGCCAC GTGGAAATAA CCTCAGTACC ATCTCTGATA CCAGCCCCAT GAAGCGCTCA 
GCCTCCGTGC TGGGACCCAA AGCCCGGCGA CTGGATGACT ACTCACTAGA GCGGGTACCA 
CCTGAGGAGA ACCAAAGGTA CCACCAACGC CGCCGGGACC GTGGCCACCG CACCTCTGAG 
CGCTCTCTGG GCCGATACAC TGATGTGGAC ACAGGCCTGG GGACAGATCT GAGCATGACC 
ACCCAATCGG GTGACCTGCC CTCCAAAGAT CGGGACCAGG ACCGGGGCCG GCCCAAGGAC 
CGGAAGCATC GGCCACACCA CCACCACCAC CATCATCACC ATCATCCCCC GGCCCCGGAC 
CGGGAGCGCT ACGCACAGGA GCGGCCGGAC ACCGGCCGGG CGCGGGCCCG GGAGCAGCGC 
TGGTCCCGCT CGCCCAGCGA GGGTCGGGAG CACGCGACAC ACAGACAGGG CAGTAGTTCC 
GTCAGTGGAA GCCCAGCCCC CTCTACGTCC GGCACCAGCA CGCCGCGGCG CGGCCGCCGC 
CAGCTCCCCC AGACCCCCTG CACCCCGCGG CCGCTTGTGT CCTACTCGCC CGCTCCGCGC 
AGGCCTGCGG CGCGCAGGAT GGCGGGTCCC CCAGCGCCCC CTGGCGGCTC GCCGCGGGGT 
TGCCGCCGTG CGCCGCGCTG GCCTGCGCAT GCGCCTGAGG GCCCGCGGCC GCGCGGTGCC 
GACTACACCG AGCCCGACAG CCCGCGCGAG CCGCCAGGGG GCGCGCATGA GCCCGCGCCG 
CGGTCGCCCA GGACTCCGCG CGCCGCGGGC TGCGCGTCAC CGCGCCACGG CCGCAGGCTG 
CCCAATGGTT ACTACGCGGG GCACGGCGCG CCACGGCCGC GCACAGCCCG CAGGGGCGCG 
CACGACGCGT ACAGCGAGAG CGAGGACGAC TGGTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008770570.2
CDS	108..7646
Translation	MVSSPGISERGPWMPRASWVSDPMRSPLNHELLHSSRYPGTDCTLAGSSRTLHVPWSEQMSGSLEGPTLKRRGGVVDHRDVILAHQAHKMHSTPQARRKEWEMARFGDEMPGRYGAGGGGSGPAAGVVVGAAGGRGAGGSRQGGQPGAQRMYKQSMAQRARTMALYNPIPVRQNCLTVNRSLFLFSEDNVVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDDDKTPMSERLDDTEPYFIGIFCFEAGIKIVALGFAFHKGSYLRNGWNVMDFVVVLTGILATVGTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILIFAIIGLEFYMGKFHTTCFEEGTDDIQGESPAPCGTEEPARTCPNGTKCQPYWEGPNNGITQFDNILFAVLTVFQCITMEGWTDLLYNSNDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYMEWISKAEEVILAEDETDVEQRHPFDGALRRATLKKSKTDLLNPEEAEDQLADIASVGSPFARASIKSAKLENSTFFHKKERRMRFYIRRMVKTQAFYWTVLSLVALNTLCVAIVHYNQPEWLSDFLYYAEFIFLGLFMSEMFIKMYGLGTRPYFHSSFNCFDCGVIIGSIFEVIWAVIKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFDEGTPPTNFDTFPAAIMTVFQILTGEDWNEVMYDGIKSQGGVQGGMVFSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAANQKLALQKAKEVAEVSPLSAANMSIAVKEQQKNQKPAKSVWEQRTSEMRKQNLLASREALYGDAAERWPTTYARPLRPDVKTHLDRPLVVDPQENRNNNTNKSRAPEALRQTARPRESARDPDARRAWPSSPERAPGREGPYGRESEPQQREHAPPREHVPWDADPERAKAGDAPRRHTHRPVAEGEPRRHRARRRPGDEPDDRPERRPRPRDATRPARAADGEGDDGERKRRHRHGPPAHDDRERRHRRRKESQGSGVPMSGPNLSTTRPIQQDLGRQDLPLAEDLDNMKNNKLATGEPASPHDSLGHSGLPPSPAKIGNSTNPGPALATNPQNAASRRTPNNPGNPSNPGPPKTPENSLIVTNPSSTQPNSAKTARKPEHMAVEIPPACPPLNHTVVQVNKNANPDPLPKKEEEKKEEEEADPGEDGPKPMPPYSSMFILSTTNPLRRLCHYILNLRYFEMCILMVIAMSSIALAAEDPVQPNAPRNNVLRYFDYVFTGVFTFEMVIKMIDLGLVLHQGAYFRDLWNILDFIVVSGALVAFAFTSSTRGNSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYMLFMFIFAVVAVQLFKGKFFHCTDESKEFERDCRGKYLLYEKNEVKARDREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATFENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEEYSLEKNERACIDFAISAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYTIMAMIALNTIVLMMKFYGASVAYENALRVFNIVFTSLFSLECVLKVMAFGILNYFRDAWNIFDFVTVLGSITDILVTEFGNPNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVIPHPWGTGAAGGSLCQREPRWLWLLPSSPPPPTLQVFGNIGIDGEDEDSDEDEFQITEHNNFRTFFQALMLLFRSATGEAWHNIMLSCLSGKPCDKNSGIQKPECGNEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRVWAEYDPAAWGRMPYPDMYQMLRHMSPPLGLGKKCPARVAYKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKQQMDAELRKEMMAIWPNLSQKTLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKKLQAMREEQNRTPLMFQRMEPPSPTQEGGPSQNALPSTQLDPGGGLMAQESSMKESPSWVTQRAQEMFQKTGTWSPERGPPIDMPNSQPNSQSVEMREMGTDGYSDSEHYLPMEGQTRAASMPRLPAENQRRRGRPRGNNLSTISDTSPMKRSASVLGPKARRLDDYSLERVPPEENQRYHQRRRDRGHRTSERSLGRYTDVDTGLGTDLSMTTQSGDLPSKDRDQDRGRPKDRKHRPHHHHHHHHHHPPAPDRERYAQERPDTGRARAREQRWSRSPSEGREHATHRQGSSSVSGSPAPSTSGTSTPRRGRRQLPQTPCTPRPLVSYSPAPRRPAARRMAGPPAPPGGSPRGCRRAPRWPAHAPEGPRPRGADYTEPDSPREPPGGAHEPAPRSPRTPRAAGCASPRHGRRLPNGYYAGHGAPRPRTARRGAHDAYSESEDDWC

Target ORF information:

RefSeq Version	XM_008772348.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel subunit alpha1 A (Cacna1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772348.2

ORF Insert Sequence:

ATGGTTTCCT CTCCTGGGAT TTCTGAGCGA GGCCCATGGA TGCCGAGGGC CTCCTGGGTC 
TCTGACCCCA TGCGTTCTCC CCTCAATCAT GAGCTCCTGC ACAGCTCGAG GTACCCAGGC 
ACGGACTGCA CCCTGGCAGG TTCTTCCAGG ACTTTGCATG TCCCGTGGTC CGAGCAGATG 
AGCGGAAGCC TCGAGGGGCC AACTCTGAAA CGGCGTGGTG GCGTGGTGGA CCACCGGGAT 
GTCATCTTGG CCCACCAGGC GCACAAAATG CACAGCACCC CTCAGGCCAG GAGGAAAGAG 
TGGGAAATGG CCCGCTTTGG AGACGAGATG CCGGGCCGCT ACGGCGCAGG CGGAGGAGGC 
TCAGGGCCGG CCGCCGGGGT GGTCGTGGGC GCCGCGGGCG GCCGAGGAGC CGGGGGCAGC 
CGGCAGGGCG GGCAGCCCGG AGCGCAGAGG ATGTACAAGC AGTCGATGGC GCAGAGAGCG 
CGGACCATGG CCCTCTACAA CCCCATCCCT GTCCGCCAGA ACTGCCTCAC GGTCAACCGC 
TCCCTGTTCC TCTTCAGTGA AGACAACGTG GTGAGAAAAT ACGCCAAAAA GATCACGGAA 
TGGCCTCCCT TCGAGTACAT GATCCTGGCC ACCATCATTG CTAACTGCAT CGTCCTGGCC 
CTGGAGCAGC ACCTCCCTGA TGATGACAAG ACACCCATGT CCGAGCGGCT GGATGACACA 
GAACCCTATT TCATTGGCAT CTTCTGTTTT GAGGCTGGAA TTAAGATCGT GGCTCTTGGC 
TTTGCCTTCC ACAAAGGCTC CTACCTGAGG AATGGCTGGA ACGTCATGGA CTTTGTCGTG 
GTGCTAACAG GCATCTTGGC CACTGTCGGG ACGGAGTTTG ATCTACGGAC ACTGAGGGCG 
GTTCGTGTGC TGCGGCCACT CAAGCTGGTG TCTGGAATCC CAAGTTTACA AGTCGTCCTG 
AAGTCAATCA TGAAGGCGAT GATCCCTCTG CTGCAGATCG GCCTCCTCCT GTTTTTTGCA 
ATCCTTATTT TTGCAATCAT AGGGTTAGAA TTTTATATGG GAAAATTTCA TACCACCTGC 
TTTGAAGAGG GGACAGACGA CATCCAGGGT GAGTCGCCAG CTCCGTGTGG GACAGAGGAG 
CCTGCCCGCA CCTGCCCCAA CGGGACCAAA TGTCAGCCGT ACTGGGAAGG GCCCAACAAC 
GGCATCACTC AGTTCGACAA CATCCTGTTT GCTGTGCTCA CTGTTTTCCA GTGCATCACC 
ATGGAAGGCT GGACTGATCT CCTCTACAAT AGCAACGATG CCTCAGGGAA CACTTGGAAC 
TGGTTGTACT TCATCCCCCT CATCATCATC GGCTCCTTTT TTATGCTGAA CCTTGTGCTG 
GGTGTGCTGT CTGGGGAGTT TGCCAAAGAA AGGGAACGTG TAGAGAACCG AAGGGCTTTT 
CTGAAGCTCA GAAGACAACA GCAGATTGAA CGTGAGCTCA ATGGATACAT GGAGTGGATC 
TCGAAAGCAG AAGAGGTGAT CCTCGCGGAG GACGAGACAG ACGTGGAGCA GAGGCACCCT 
TTTGATGGAG CTCTTCGGAG AGCTACTCTG AAGAAAAGCA AGACGGACCT GCTCAACCCT 
GAGGAGGCGG AGGACCAGCT TGCTGACATC GCGTCTGTGG GGTCTCCCTT CGCCAGAGCC 
AGCATCAAAA GTGCCAAGCT GGAGAATTCG ACTTTTTTCC ACAAAAAGGA GAGAAGAATG 
CGTTTCTACA TCCGCCGCAT GGTCAAAACT CAGGCCTTCT ACTGGACCGT GCTCAGTCTG 
GTAGCCCTCA ACACGCTGTG CGTCGCCATT GTCCACTACA ACCAGCCCGA GTGGCTCTCC 
GACTTCCTCT ACTATGCAGA ATTCATTTTC TTAGGACTCT TTATGTCCGA AATGTTTATA 
AAAATGTATG GGCTCGGGAC ACGGCCTTAC TTCCACTCTT CCTTCAACTG CTTTGACTGT 
GGGGTCATCA TCGGGAGCAT CTTTGAAGTC ATCTGGGCCG TCATCAAACC GGGTACATCC 
TTTGGAATCA GCGTGTTACG AGCTCTCAGG TTACTGCGTA TTTTCAAAGT CACAAAGTAC 
TGGGCATCTC TCAGAAACCT GGTTGTCTCC CTCCTCAACT CCATGAAATC CATCATAAGT 
CTGCTGTTCC TCCTCTTCCT CTTCATTGTC GTCTTTGCCC TCTTGGGGAT GCAGCTGTTT 
GGTGGCCAGT TTAATTTTGA CGAGGGGACT CCTCCCACCA ACTTCGACAC TTTTCCAGCA 
GCAATAATGA CTGTGTTTCA GATCCTGACT GGCGAGGATT GGAATGAGGT CATGTATGAT 
GGGATCAAGT CTCAGGGGGG CGTGCAGGGC GGCATGGTGT TCTCCATCTA CTTCATCGTC 
CTCACCCTCT TCGGGAACTA CACCCTGCTG AACGTGTTCT TGGCTATCGC GGTGGACAAC 
CTGGCCAACG CCCAGGAACT CACCAAGGAT GAACAAGAAG AGGAAGAGGC AGCCAATCAG 
AAACTGGCTC TACAGAAAGC CAAGGAGGTG GCAGAAGTGA GTCCCCTGTC TGCAGCCAAC 
ATGTCCATAG CTGTGAAGGA ACAGCAGAAG AACCAGAAGC CTGCCAAGTC GGTGTGGGAG 
CAGCGCACCA GCGAGATGCG CAAGCAGAAC CTGCTGGCTA GCCGCGAGGC GCTGTACGGG 
GACGCGGCTG AGCGCTGGCC CACCACTTAC GCGCGCCCGC TGCGGCCGGA CGTGAAGACG 
CACTTGGACC GGCCGCTCGT GGTGGACCCG CAGGAGAACC GTAACAACAA CACCAACAAG 
AGCCGTGCGC CAGAAGCGCT GCGCCAAACC GCGCGGCCCC GCGAGAGCGC GCGCGACCCC 
GACGCGCGGC GCGCCTGGCC CAGCAGCCCT GAGCGCGCCC CTGGACGAGA GGGCCCGTAT 
GGCCGCGAGA GCGAGCCGCA ACAGCGCGAG CACGCGCCAC CCCGCGAGCA CGTACCCTGG 
GACGCGGATC CTGAGCGCGC CAAGGCCGGG GACGCGCCCC GCCGCCACAC GCACCGGCCT 
GTGGCCGAGG GCGAGCCTCG TCGCCACCGC GCGCGCCGCC GGCCCGGGGA CGAACCGGAC 
GACAGACCGG AGCGCAGGCC GCGTCCCCGC GACGCCACTA GGCCGGCCCG CGCTGCAGAC 
GGCGAAGGCG ATGATGGGGA GCGCAAGCGG CGACACCGAC ACGGGCCGCC GGCCCACGAT 
GACAGGGAGC GCAGACACCG GCGCAGAAAA GAGAGCCAGG GCTCTGGGGT CCCCATGTCT 
GGTCCCAACC TGTCCACCAC CAGGCCAATC CAGCAGGATC TGGGCCGCCA GGACCTGCCA 
CTGGCTGAGG ACCTGGACAA CATGAAGAAC AACAAGTTGG CCACCGGGGA GCCTGCCAGT 
CCCCACGACA GCCTGGGCCA CAGTGGCCTT CCCCCTAGCC CTGCCAAGAT CGGGAACAGC 
ACCAACCCTG GTCCCGCCTT GGCCACCAAT CCCCAGAATG CTGCCAGCCG CAGGACGCCC 
AACAACCCGG GCAACCCGTC CAACCCCGGC CCCCCCAAGA CTCCCGAGAA CAGCCTTATC 
GTCACCAACC CCAGCAGCAC CCAGCCCAAC TCAGCAAAGA CTGCCAGGAA ACCCGAGCAC 
ATGGCGGTGG AGATCCCCCC GGCCTGCCCG CCCCTCAACC ACACTGTGGT CCAAGTAAAC 
AAAAACGCCA ACCCAGACCC ACTGCCAAAG AAAGAGGAAG AGAAGAAGGA GGAAGAGGAG 
GCAGACCCCG GGGAGGATGG CCCAAAGCCC ATGCCGCCCT ACAGCTCCAT GTTCATCCTC 
TCCACCACCA ACCCCCTTCG CCGGCTGTGC CATTACATCC TGAACCTGCG CTACTTCGAG 
ATGTGCATCC TCATGGTCAT TGCCATGAGT AGCATCGCGC TGGCCGCCGA GGACCCGGTG 
CAGCCCAACG CACCCCGCAA CAACGTGCTG CGATATTTTG ACTATGTTTT CACAGGAGTG 
TTTACCTTTG AGATGGTGAT CAAGATGATC GACCTGGGCC TCGTCCTGCA TCAGGGGGCC 
TATTTCCGTG ACCTGTGGAA CATTCTGGAC TTCATAGTGG TCAGTGGGGC CCTGGTGGCC 
TTTGCCTTCA CGAGCAGTAC ACGTGGCAAT AGCAAAGGAA AGGACATCAA CACCATCAAG 
TCCCTCCGAG TCCTCCGGGT GCTACGACCT CTAAAGACCA TCAAGCGGCT GCCTAAGTTG 
AAGGCCGTAT TTGACTGCGT GGTGAACTCG CTCAAGAACG TCTTCAACAT CCTCATTGTC 
TACATGCTCT TCATGTTCAT CTTCGCCGTG GTGGCCGTGC AGCTCTTCAA GGGCAAATTC 
TTCCACTGCA CGGACGAGTC CAAGGAGTTT GAGAGAGACT GTCGAGGCAA ATACCTCCTT 
TACGAGAAGA ACGAGGTAAA GGCGCGGGAC CGCGAGTGGA AGAAATACGA CTTCCACTAC 
GACAACGTGC TCTGGGCCCT GCTCACGCTC TTTACGGTGT CCACGGGAGA GGGCTGGCCA 
CAGGTCCTCA AGCACTCAGT GGATGCCACT TTTGAGAACC AGGGCCCCAG CCCCGGGTAC 
CGCATGGAAA TGTCCATCTT CTACGTGGTC TACTTTGTGG TGTTTCCCTT CTTCTTTGTC 
AATATCTTTG TGGCCTTGAT CATCATCACC TTCCAGGAGC AGGGAGACAA GATGATGGAA 
GAATACAGCC TAGAGAAAAA TGAGAGGGCC TGCATCGACT TTGCCATCAG TGCCAAGCCG 
CTGACCAGGC ACATGCCCCA GAACAAGCAG AGCTTCCAGT ATCGAATGTG GCAGTTCGTG 
GTGTCCCCAC CCTTTGAGTA CACCATCATG GCCATGATCG CTCTCAACAC CATCGTGCTA 
ATGATGAAGT TCTATGGAGC CTCTGTGGCC TATGAAAACG CCCTTCGAGT GTTCAACATT 
GTCTTCACCT CCCTCTTCTC TCTCGAATGT GTGCTCAAAG TCATGGCTTT TGGGATTCTG 
AATTATTTCC GCGATGCCTG GAACATCTTC GACTTTGTGA CTGTTCTGGG CAGCATCACA 
GACATCCTCG TCACCGAGTT TGGGAATCCG AATAACTTCA TCAACCTGAG CTTTCTCCGC 
CTCTTCCGTG CTGCCCGACT CATCAAACTC CTCCGCCAGG GTTACACCAT CCGCATTCTC 
CTCTGGACTT TCGTGCAGTC TTTCAAGGCC CTACCTTATG TCTGTCTGCT GATCGCCATG 
CTCTTCTTCA TCTATGCCAT CATCGGGATG CAGGTGATCC CGCACCCTTG GGGTACCGGG 
GCTGCGGGAG GGAGCTTGTG TCAAAGGGAG CCAAGGTGGC TGTGGCTGCT CCCATCGTCA 
CCCCCCCCCC CAACTCTCCA GGTGTTTGGC AACATCGGCA TTGATGGGGA AGATGAGGAC 
AGCGATGAGG ATGAGTTCCA AATCACGGAG CACAATAACT TCCGGACCTT CTTCCAAGCT 
CTCATGCTTC TCTTCCGGAG CGCCACAGGG GAAGCGTGGC ACAACATCAT GCTGTCCTGC 
CTCAGCGGGA AGCCATGCGA CAAGAACTCC GGGATCCAAA AACCAGAGTG TGGCAACGAG 
TTCGCCTATT TTTACTTTGT CTCGTTCATC TTCCTTTGCT CATTTCTGAT GCTGAATCTC 
TTTGTTGCTG TCATCATGGA CAACTTCGAG TACCTCACCC GAGATTCCTC CATCCTGGGC 
CCCCACCACC TGGATGAGTA CGTGCGTGTC TGGGCAGAGT ATGACCCTGC TGCCTGGGGT 
CGCATGCCTT ACCCGGACAT GTATCAGATG CTGAGACACA TGTCCCCGCC CCTGGGTCTT 
GGGAAGAAGT GCCCGGCCAG AGTGGCTTAC AAGAGGCTCT TGCGGATGGA CCTACCCGTA 
GCGGATGACA ACACCGTTCA CTTCAACTCC ACCTTGATGG CTCTGATCCG AACCGCCCTG 
GATATCAAAA TCGCAAAGGG TGGAGCTGAC AAGCAGCAAA TGGACGCAGA GCTCCGCAAG 
GAAATGATGG CCATTTGGCC CAACCTGTCT CAGAAGACCT TGGATCTGCT GGTCACACCT 
CACAAGTCCA CGGACCTGAC AGTGGGTAAG ATCTACGCAG CCATGATGAT CATGGAGTAC 
TACCGGCAGA GCAAGGCCAA GAAGCTGCAG GCCATGCGAG AGGAGCAGAA CCGGACACCA 
CTCATGTTCC AGCGCATGGA GCCTCCATCG CCAACACAGG AGGGAGGACC CAGCCAAAAC 
GCCCTTCCCT CCACTCAGCT GGACCCCGGA GGAGGCCTGA TGGCTCAAGA AAGCAGCATG 
AAGGAGAGCC CGTCCTGGGT GACCCAGCGG GCACAGGAGA TGTTCCAGAA GACTGGTACC 
TGGAGCCCAG AGCGAGGGCC ACCCATCGAC ATGCCTAACA GCCAGCCCAA CTCCCAGTCT 
GTGGAGATGA GAGAAATGGG AACTGATGGC TACTCAGACA GCGAACACTA CCTCCCCATG 
GAAGGACAGA CCAGGGCCGC CTCCATGCCC CGCCTCCCAG CAGAGAACCA GAGGAGAAGG 
GGCCGGCCAC GTGGAAATAA CCTCAGTACC ATCTCTGATA CCAGCCCCAT GAAGCGCTCA 
GCCTCCGTGC TGGGACCCAA AGCCCGGCGA CTGGATGACT ACTCACTAGA GCGGGTACCA 
CCTGAGGAGA ACCAAAGGTA CCACCAACGC CGCCGGGACC GTGGCCACCG CACCTCTGAG 
CGCTCTCTGG GCCGATACAC TGATGTGGAC ACAGGCCTGG GGACAGATCT GAGCATGACC 
ACCCAATCGG GTGACCTGCC CTCCAAAGAT CGGGACCAGG ACCGGGGCCG GCCCAAGGAC 
CGGAAGCATC GGCCACACCA CCACCACCAC CATCATCACC ATCATCCCCC GGCCCCGGAC 
CGGGAGCGCT ACGCACAGGA GCGGCCGGAC ACCGGCCGGG CGCGGGCCCG GGAGCAGCGC 
TGGTCCCGCT CGCCCAGCGA GGGTCGGGAG CACGCGACAC ACAGACAGGG CAGTAGTTCC 
GTCAGTGGAA GCCCAGCCCC CTCTACGTCC GGCACCAGCA CGCCGCGGCG CGGCCGCCGC 
CAGCTCCCCC AGACCCCCTG CACCCCGCGG CCGCTTGTGT CCTACTCGCC CGCTCCGCGC 
AGGCCTGCGG CGCGCAGGAT GGCGGGTCCC CCAGCGCCCC CTGGCGGCTC GCCGCGGGGT 
TGCCGCCGTG CGCCGCGCTG GCCTGCGCAT GCGCCTGAGG GCCCGCGGCC GCGCGGTGCC 
GACTACACCG AGCCCGACAG CCCGCGCGAG CCGCCAGGGG GCGCGCATGA GCCCGCGCCG 
CGGTCGCCCA GGACTCCGCG CGCCGCGGGC TGCGCGTCAC CGCGCCACGG CCGCAGGCTG 
CCCAATGGTT ACTACGCGGG GCACGGCGCG CCACGGCCGC GCACAGCCCG CAGGGGCGCG 
CACGACGCGT ACAGCGAGAG CGAGGACGAC TGGTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1a ( XM_008772349.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008770571.2 Rattus norvegicus calcium voltage-gated channel subunit alpha1 A (Cacna1a), transcript variant X3, mRNA.

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CloneID	ORa59366
Clone ID Related Accession (Same CDS sequence)	XM_008772349.2
Accession Version	XM_008772349.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008772349.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTCCT CTCCTGGGAT TTCTGAGCGA GGCCCATGGA TGCCGAGGGC CTCCTGGGTC 
TCTGACCCCA TGCGTTCTCC CCTCAATCAT GAGCTCCTGC ACAGCTCGAG GTACCCAGGC 
ACGGACTGCA CCCTGGCAGG TTCTTCCAGG ACTTTGCATG TCCCGTGGTC CGAGCAGATG 
AGCGGAAGCC TCGAGGGGCC AACTCTGAAA CGGCGTGGTG GCGTGGTGGA CCACCGGGAT 
GTCATCTTGG CCCACCAGGC GCACAAAATG CACAGCACCC CTCAGGCCAG GAGGAAAGAG 
TGGGAAATGG CCCGCTTTGG AGACGAGATG CCGGGCCGCT ACGGCGCAGG CGGAGGAGGC 
TCAGGGCCGG CCGCCGGGGT GGTCGTGGGC GCCGCGGGCG GCCGAGGAGC CGGGGGCAGC 
CGGCAGGGCG GGCAGCCCGG AGCGCAGAGG ATGTACAAGC AGTCGATGGC GCAGAGAGCG 
CGGACCATGG CCCTCTACAA CCCCATCCCT GTCCGCCAGA ACTGCCTCAC GGTCAACCGC 
TCCCTGTTCC TCTTCAGTGA AGACAACGTG GTGAGAAAAT ACGCCAAAAA GATCACGGAA 
TGGCCTCCCT TCGAGTACAT GATCCTGGCC ACCATCATTG CTAACTGCAT CGTCCTGGCC 
CTGGAGCAGC ACCTCCCTGA TGATGACAAG ACACCCATGT CCGAGCGGCT GGATGACACA 
GAACCCTATT TCATTGGCAT CTTCTGTTTT GAGGCTGGAA TTAAGATCGT GGCTCTTGGC 
TTTGCCTTCC ACAAAGGCTC CTACCTGAGG AATGGCTGGA ACGTCATGGA CTTTGTCGTG 
GTGCTAACAG GCATCTTGGC CACTGTCGGG ACGGAGTTTG ATCTACGGAC ACTGAGGGCG 
GTTCGTGTGC TGCGGCCACT CAAGCTGGTG TCTGGAATCC CAAGTTTACA AGTCGTCCTG 
AAGTCAATCA TGAAGGCGAT GATCCCTCTG CTGCAGATCG GCCTCCTCCT GTTTTTTGCA 
ATCCTTATTT TTGCAATCAT AGGGTTAGAA TTTTATATGG GAAAATTTCA TACCACCTGC 
TTTGAAGAGG GGACAGACGA CATCCAGGGT GAGTCGCCAG CTCCGTGTGG GACAGAGGAG 
CCTGCCCGCA CCTGCCCCAA CGGGACCAAA TGTCAGCCGT ACTGGGAAGG GCCCAACAAC 
GGCATCACTC AGTTCGACAA CATCCTGTTT GCTGTGCTCA CTGTTTTCCA GTGCATCACC 
ATGGAAGGCT GGACTGATCT CCTCTACAAT AGCAACGATG CCTCAGGGAA CACTTGGAAC 
TGGTTGTACT TCATCCCCCT CATCATCATC GGCTCCTTTT TTATGCTGAA CCTTGTGCTG 
GGTGTGCTGT CTGGGGAGTT TGCCAAAGAA AGGGAACGTG TAGAGAACCG AAGGGCTTTT 
CTGAAGCTCA GAAGACAACA GCAGATTGAA CGTGAGCTCA ATGGATACAT GGAGTGGATC 
TCGAAAGCAG AGGTGATCCT CGCGGAGGAC GAGACAGACG TGGAGCAGAG GCACCCTTTT 
GATGGAGCTC TTCGGAGAGC TACTCTGAAG AAAAGCAAGA CGGACCTGCT CAACCCTGAG 
GAGGCGGAGG ACCAGCTTGC TGACATCGCG TCTGTGGGGT CTCCCTTCGC CAGAGCCAGC 
ATCAAAAGTG CCAAGCTGGA GAATTCGACT TTTTTCCACA AAAAGGAGAG AAGAATGCGT 
TTCTACATCC GCCGCATGGT CAAAACTCAG GCCTTCTACT GGACCGTGCT CAGTCTGGTA 
GCCCTCAACA CGCTGTGCGT CGCCATTGTC CACTACAACC AGCCCGAGTG GCTCTCCGAC 
TTCCTCTACT ATGCAGAATT CATTTTCTTA GGACTCTTTA TGTCCGAAAT GTTTATAAAA 
ATGTATGGGC TCGGGACACG GCCTTACTTC CACTCTTCCT TCAACTGCTT TGACTGTGGG 
GTCATCATCG GGAGCATCTT TGAAGTCATC TGGGCCGTCA TCAAACCGGG TACATCCTTT 
GGAATCAGCG TGTTACGAGC TCTCAGGTTA CTGCGTATTT TCAAAGTCAC AAAGTACTGG 
GCATCTCTCA GAAACCTGGT TGTCTCCCTC CTCAACTCCA TGAAATCCAT CATAAGTCTG 
CTGTTCCTCC TCTTCCTCTT CATTGTCGTC TTTGCCCTCT TGGGGATGCA GCTGTTTGGT 
GGCCAGTTTA ATTTTGACGA GGGGACTCCT CCCACCAACT TCGACACTTT TCCAGCAGCA 
ATAATGACTG TGTTTCAGAT CCTGACTGGC GAGGATTGGA ATGAGGTCAT GTATGATGGG 
ATCAAGTCTC AGGGGGGCGT GCAGGGCGGC ATGGTGTTCT CCATCTACTT CATCGTCCTC 
ACCCTCTTCG GGAACTACAC CCTGCTGAAC GTGTTCTTGG CTATCGCGGT GGACAACCTG 
GCCAACGCCC AGGAACTCAC CAAGGATGAA CAAGAAGAGG AAGAGGCAGC CAATCAGAAA 
CTGGCTCTAC AGAAAGCCAA GGAGGTGGCA GAAGTGAGTC CCCTGTCTGC AGCCAACATG 
TCCATAGCTG TGAAGGAACA GCAGAAGAAC CAGAAGCCTG CCAAGTCGGT GTGGGAGCAG 
CGCACCAGCG AGATGCGCAA GCAGAACCTG CTGGCTAGCC GCGAGGCGCT GTACGGGGAC 
GCGGCTGAGC GCTGGCCCAC CACTTACGCG CGCCCGCTGC GGCCGGACGT GAAGACGCAC 
TTGGACCGGC CGCTCGTGGT GGACCCGCAG GAGAACCGTA ACAACAACAC CAACAAGAGC 
CGTGCGCCAG AAGCGCTGCG CCAAACCGCG CGGCCCCGCG AGAGCGCGCG CGACCCCGAC 
GCGCGGCGCG CCTGGCCCAG CAGCCCTGAG CGCGCCCCTG GACGAGAGGG CCCGTATGGC 
CGCGAGAGCG AGCCGCAACA GCGCGAGCAC GCGCCACCCC GCGAGCACGT ACCCTGGGAC 
GCGGATCCTG AGCGCGCCAA GGCCGGGGAC GCGCCCCGCC GCCACACGCA CCGGCCTGTG 
GCCGAGGGCG AGCCTCGTCG CCACCGCGCG CGCCGCCGGC CCGGGGACGA ACCGGACGAC 
AGACCGGAGC GCAGGCCGCG TCCCCGCGAC GCCACTAGGC CGGCCCGCGC TGCAGACGGC 
GAAGGCGATG ATGGGGAGCG CAAGCGGCGA CACCGACACG GGCCGCCGGC CCACGATGAC 
AGGGAGCGCA GACACCGGCG CAGAAAAGAG AGCCAGGGCT CTGGGGTCCC CATGTCTGGT 
CCCAACCTGT CCACCACCAG GCCAATCCAG CAGGATCTGG GCCGCCAGGA CCTGCCACTG 
GCTGAGGACC TGGACAACAT GAAGAACAAC AAGTTGGCCA CCGGGGAGCC TGCCAGTCCC 
CACGACAGCC TGGGCCACAG TGGCCTTCCC CCTAGCCCTG CCAAGATCGG GAACAGCACC 
AACCCTGGTC CCGCCTTGGC CACCAATCCC CAGAATGCTG CCAGCCGCAG GACGCCCAAC 
AACCCGGGCA ACCCGTCCAA CCCCGGCCCC CCCAAGACTC CCGAGAACAG CCTTATCGTC 
ACCAACCCCA GCAGCACCCA GCCCAACTCA GCAAAGACTG CCAGGAAACC CGAGCACATG 
GCGGTGGAGA TCCCCCCGGC CTGCCCGCCC CTCAACCACA CTGTGGTCCA AGTAAACAAA 
AACGCCAACC CAGACCCACT GCCAAAGAAA GAGGAAGAGA AGAAGGAGGA AGAGGAGGCA 
GACCCCGGGG AGGATGGCCC AAAGCCCATG CCGCCCTACA GCTCCATGTT CATCCTCTCC 
ACCACCAACC CCCTTCGCCG GCTGTGCCAT TACATCCTGA ACCTGCGCTA CTTCGAGATG 
TGCATCCTCA TGGTCATTGC CATGAGTAGC ATCGCGCTGG CCGCCGAGGA CCCGGTGCAG 
CCCAACGCAC CCCGCAACAA CGTGCTGCGA TATTTTGACT ATGTTTTCAC AGGAGTGTTT 
ACCTTTGAGA TGGTGATCAA GATGATCGAC CTGGGCCTCG TCCTGCATCA GGGGGCCTAT 
TTCCGTGACC TGTGGAACAT TCTGGACTTC ATAGTGGTCA GTGGGGCCCT GGTGGCCTTT 
GCCTTCACGA GCAGTACACG TGGCAATAGC AAAGGAAAGG ACATCAACAC CATCAAGTCC 
CTCCGAGTCC TCCGGGTGCT ACGACCTCTA AAGACCATCA AGCGGCTGCC TAAGTTGAAG 
GCCGTATTTG ACTGCGTGGT GAACTCGCTC AAGAACGTCT TCAACATCCT CATTGTCTAC 
ATGCTCTTCA TGTTCATCTT CGCCGTGGTG GCCGTGCAGC TCTTCAAGGG CAAATTCTTC 
CACTGCACGG ACGAGTCCAA GGAGTTTGAG AGAGACTGTC GAGGCAAATA CCTCCTTTAC 
GAGAAGAACG AGGTAAAGGC GCGGGACCGC GAGTGGAAGA AATACGACTT CCACTACGAC 
AACGTGCTCT GGGCCCTGCT CACGCTCTTT ACGGTGTCCA CGGGAGAGGG CTGGCCACAG 
GTCCTCAAGC ACTCAGTGGA TGCCACTTTT GAGAACCAGG GCCCCAGCCC CGGGTACCGC 
ATGGAAATGT CCATCTTCTA CGTGGTCTAC TTTGTGGTGT TTCCCTTCTT CTTTGTCAAT 
ATCTTTGTGG CCTTGATCAT CATCACCTTC CAGGAGCAGG GAGACAAGAT GATGGAAGAA 
TACAGCCTAG AGAAAAATGA GAGGGCCTGC ATCGACTTTG CCATCAGTGC CAAGCCGCTG 
ACCAGGCACA TGCCCCAGAA CAAGCAGAGC TTCCAGTATC GAATGTGGCA GTTCGTGGTG 
TCCCCACCCT TTGAGTACAC CATCATGGCC ATGATCGCTC TCAACACCAT CGTGCTAATG 
ATGAAGTTCT ATGGAGCCTC TGTGGCCTAT GAAAACGCCC TTCGAGTGTT CAACATTGTC 
TTCACCTCCC TCTTCTCTCT CGAATGTGTG CTCAAAGTCA TGGCTTTTGG GATTCTGAAT 
TATTTCCGCG ATGCCTGGAA CATCTTCGAC TTTGTGACTG TTCTGGGCAG CATCACAGAC 
ATCCTCGTCA CCGAGTTTGG GAATCCGAAT AACTTCATCA ACCTGAGCTT TCTCCGCCTC 
TTCCGTGCTG CCCGACTCAT CAAACTCCTC CGCCAGGGTT ACACCATCCG CATTCTCCTC 
TGGACTTTCG TGCAGTCTTT CAAGGCCCTA CCTTATGTCT GTCTGCTGAT CGCCATGCTC 
TTCTTCATCT ATGCCATCAT CGGGATGCAG GTGATCCCGC ACCCTTGGGG TACCGGGGCT 
GCGGGAGGGA GCTTGTGTCA AAGGGAGCCA AGGTGGCTGT GGCTGCTCCC ATCGTCACCC 
CCCCCCCCAA CTCTCCAGGT GTTTGGCAAC ATCGGCATTG ATGGGGAAGA TGAGGACAGC 
GATGAGGATG AGTTCCAAAT CACGGAGCAC AATAACTTCC GGACCTTCTT CCAAGCTCTC 
ATGCTTCTCT TCCGGAGCGC CACAGGGGAA GCGTGGCACA ACATCATGCT GTCCTGCCTC 
AGCGGGAAGC CATGCGACAA GAACTCCGGG ATCCAAAAAC CAGAGTGTGG CAACGAGTTC 
GCCTATTTTT ACTTTGTCTC GTTCATCTTC CTTTGCTCAT TTCTGATGCT GAATCTCTTT 
GTTGCTGTCA TCATGGACAA CTTCGAGTAC CTCACCCGAG ATTCCTCCAT CCTGGGCCCC 
CACCACCTGG ATGAGTACGT GCGTGTCTGG GCAGAGTATG ACCCTGCTGC CTGCGGCCGC 
ATTCACTATA AGGACATGTA CAGTTTATTG CGAGTAATAT CGCCCCCTCT CGGCTTAGGC 
AAGAAATGTC CTCATAGGGT TGCTTGCAAG AGGCTCTTGC GGATGGACCT ACCCGTAGCG 
GATGACAACA CCGTTCACTT CAACTCCACC TTGATGGCTC TGATCCGAAC CGCCCTGGAT 
ATCAAAATCG CAAAGGGTGG AGCTGACAAG CAGCAAATGG ACGCAGAGCT CCGCAAGGAA 
ATGATGGCCA TTTGGCCCAA CCTGTCTCAG AAGACCTTGG ATCTGCTGGT CACACCTCAC 
AAGTCCACGG ACCTGACAGT GGGTAAGATC TACGCAGCCA TGATGATCAT GGAGTACTAC 
CGGCAGAGCA AGGCCAAGAA GCTGCAGGCC ATGCGAGAGG AGCAGAACCG GACACCACTC 
ATGTTCCAGC GCATGGAGCC TCCATCGCCA ACACAGGAGG GAGGACCCAG CCAAAACGCC 
CTTCCCTCCA CTCAGCTGGA CCCCGGAGGA GGCCTGATGG CTCAAGAAAG CAGCATGAAG 
GAGAGCCCGT CCTGGGTGAC CCAGCGGGCA CAGGAGATGT TCCAGAAGAC TGGTACCTGG 
AGCCCAGAGC GAGGGCCACC CATCGACATG CCTAACAGCC AGCCCAACTC CCAGTCTGTG 
GAGATGAGAG AAATGGGAAC TGATGGCTAC TCAGACAGCG AACACTACCT CCCCATGGAA 
GGACAGACCA GGGCCGCCTC CATGCCCCGC CTCCCAGCAG AGAACCAGAG GAGAAGGGGC 
CGGCCACGTG GAAATAACCT CAGTACCATC TCTGATACCA GCCCCATGAA GCGCTCAGCC 
TCCGTGCTGG GACCCAAAGC CCGGCGACTG GATGACTACT CACTAGAGCG GGTACCACCT 
GAGGAGAACC AAAGGTACCA CCAACGCCGC CGGGACCGTG GCCACCGCAC CTCTGAGCGC 
TCTCTGGGCC GATACACTGA TGTGGACACA GGCCTGGGGA CAGATCTGAG CATGACCACC 
CAATCGGGTG ACCTGCCCTC CAAAGATCGG GACCAGGACC GGGGCCGGCC CAAGGACCGG 
AAGCATCGGC CACACCACCA CCACCACCAT CATCACCATC ATCCCCCGGC CCCGGACCGG 
GAGCGCTACG CACAGGAGCG GCCGGACACC GGCCGGGCGC GGGCCCGGGA GCAGCGCTGG 
TCCCGCTCGC CCAGCGAGGG TCGGGAGCAC GCGACACACA GACAGGGCAG TAGTTCCGTC 
AGTGGAAGCC CAGCCCCCTC TACGTCCGGC ACCAGCACGC CGCGGCGCGG CCGCCGCCAG 
CTCCCCCAGA CCCCCTGCAC CCCGCGGCCG CTTGTGTCCT ACTCGCCCGC TCCGCGCAGG 
CCTGCGGCGC GCAGGATGGC GGGTCCCCCA GCGCCCCCTG GCGGCTCGCC GCGGGGTTGC 
CGCCGTGCGC CGCGCTGGCC TGCGCATGCG CCTGAGGGCC CGCGGCCGCG CGGTGCCGAC 
TACACCGAGC CCGACAGCCC GCGCGAGCCG CCAGGGGGCG CGCATGAGCC CGCGCCGCGG 
TCGCCCAGGA CTCCGCGCGC CGCGGGCTGC GCGTCACCGC GCCACGGCCG CAGGCTGCCC 
AATGGTTACT ACGCGGGGCA CGGCGCGCCA CGGCCGCGCA CAGCCCGCAG GGGCGCGCAC 
GACGCGTACA GCGAGAGCGA GGACGACTGG TGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008770571.2
CDS	107..7642
Translation	MVSSPGISERGPWMPRASWVSDPMRSPLNHELLHSSRYPGTDCTLAGSSRTLHVPWSEQMSGSLEGPTLKRRGGVVDHRDVILAHQAHKMHSTPQARRKEWEMARFGDEMPGRYGAGGGGSGPAAGVVVGAAGGRGAGGSRQGGQPGAQRMYKQSMAQRARTMALYNPIPVRQNCLTVNRSLFLFSEDNVVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDDDKTPMSERLDDTEPYFIGIFCFEAGIKIVALGFAFHKGSYLRNGWNVMDFVVVLTGILATVGTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILIFAIIGLEFYMGKFHTTCFEEGTDDIQGESPAPCGTEEPARTCPNGTKCQPYWEGPNNGITQFDNILFAVLTVFQCITMEGWTDLLYNSNDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYMEWISKAEVILAEDETDVEQRHPFDGALRRATLKKSKTDLLNPEEAEDQLADIASVGSPFARASIKSAKLENSTFFHKKERRMRFYIRRMVKTQAFYWTVLSLVALNTLCVAIVHYNQPEWLSDFLYYAEFIFLGLFMSEMFIKMYGLGTRPYFHSSFNCFDCGVIIGSIFEVIWAVIKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFDEGTPPTNFDTFPAAIMTVFQILTGEDWNEVMYDGIKSQGGVQGGMVFSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAANQKLALQKAKEVAEVSPLSAANMSIAVKEQQKNQKPAKSVWEQRTSEMRKQNLLASREALYGDAAERWPTTYARPLRPDVKTHLDRPLVVDPQENRNNNTNKSRAPEALRQTARPRESARDPDARRAWPSSPERAPGREGPYGRESEPQQREHAPPREHVPWDADPERAKAGDAPRRHTHRPVAEGEPRRHRARRRPGDEPDDRPERRPRPRDATRPARAADGEGDDGERKRRHRHGPPAHDDRERRHRRRKESQGSGVPMSGPNLSTTRPIQQDLGRQDLPLAEDLDNMKNNKLATGEPASPHDSLGHSGLPPSPAKIGNSTNPGPALATNPQNAASRRTPNNPGNPSNPGPPKTPENSLIVTNPSSTQPNSAKTARKPEHMAVEIPPACPPLNHTVVQVNKNANPDPLPKKEEEKKEEEEADPGEDGPKPMPPYSSMFILSTTNPLRRLCHYILNLRYFEMCILMVIAMSSIALAAEDPVQPNAPRNNVLRYFDYVFTGVFTFEMVIKMIDLGLVLHQGAYFRDLWNILDFIVVSGALVAFAFTSSTRGNSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYMLFMFIFAVVAVQLFKGKFFHCTDESKEFERDCRGKYLLYEKNEVKARDREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATFENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEEYSLEKNERACIDFAISAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYTIMAMIALNTIVLMMKFYGASVAYENALRVFNIVFTSLFSLECVLKVMAFGILNYFRDAWNIFDFVTVLGSITDILVTEFGNPNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVIPHPWGTGAAGGSLCQREPRWLWLLPSSPPPPTLQVFGNIGIDGEDEDSDEDEFQITEHNNFRTFFQALMLLFRSATGEAWHNIMLSCLSGKPCDKNSGIQKPECGNEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRVWAEYDPAACGRIHYKDMYSLLRVISPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKQQMDAELRKEMMAIWPNLSQKTLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKKLQAMREEQNRTPLMFQRMEPPSPTQEGGPSQNALPSTQLDPGGGLMAQESSMKESPSWVTQRAQEMFQKTGTWSPERGPPIDMPNSQPNSQSVEMREMGTDGYSDSEHYLPMEGQTRAASMPRLPAENQRRRGRPRGNNLSTISDTSPMKRSASVLGPKARRLDDYSLERVPPEENQRYHQRRRDRGHRTSERSLGRYTDVDTGLGTDLSMTTQSGDLPSKDRDQDRGRPKDRKHRPHHHHHHHHHHPPAPDRERYAQERPDTGRARAREQRWSRSPSEGREHATHRQGSSSVSGSPAPSTSGTSTPRRGRRQLPQTPCTPRPLVSYSPAPRRPAARRMAGPPAPPGGSPRGCRRAPRWPAHAPEGPRPRGADYTEPDSPREPPGGAHEPAPRSPRTPRAAGCASPRHGRRLPNGYYAGHGAPRPRTARRGAHDAYSESEDDWC

Target ORF information:

RefSeq Version	XM_008772349.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel subunit alpha1 A (Cacna1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772349.2

ORF Insert Sequence:

ATGGTTTCCT CTCCTGGGAT TTCTGAGCGA GGCCCATGGA TGCCGAGGGC CTCCTGGGTC 
TCTGACCCCA TGCGTTCTCC CCTCAATCAT GAGCTCCTGC ACAGCTCGAG GTACCCAGGC 
ACGGACTGCA CCCTGGCAGG TTCTTCCAGG ACTTTGCATG TCCCGTGGTC CGAGCAGATG 
AGCGGAAGCC TCGAGGGGCC AACTCTGAAA CGGCGTGGTG GCGTGGTGGA CCACCGGGAT 
GTCATCTTGG CCCACCAGGC GCACAAAATG CACAGCACCC CTCAGGCCAG GAGGAAAGAG 
TGGGAAATGG CCCGCTTTGG AGACGAGATG CCGGGCCGCT ACGGCGCAGG CGGAGGAGGC 
TCAGGGCCGG CCGCCGGGGT GGTCGTGGGC GCCGCGGGCG GCCGAGGAGC CGGGGGCAGC 
CGGCAGGGCG GGCAGCCCGG AGCGCAGAGG ATGTACAAGC AGTCGATGGC GCAGAGAGCG 
CGGACCATGG CCCTCTACAA CCCCATCCCT GTCCGCCAGA ACTGCCTCAC GGTCAACCGC 
TCCCTGTTCC TCTTCAGTGA AGACAACGTG GTGAGAAAAT ACGCCAAAAA GATCACGGAA 
TGGCCTCCCT TCGAGTACAT GATCCTGGCC ACCATCATTG CTAACTGCAT CGTCCTGGCC 
CTGGAGCAGC ACCTCCCTGA TGATGACAAG ACACCCATGT CCGAGCGGCT GGATGACACA 
GAACCCTATT TCATTGGCAT CTTCTGTTTT GAGGCTGGAA TTAAGATCGT GGCTCTTGGC 
TTTGCCTTCC ACAAAGGCTC CTACCTGAGG AATGGCTGGA ACGTCATGGA CTTTGTCGTG 
GTGCTAACAG GCATCTTGGC CACTGTCGGG ACGGAGTTTG ATCTACGGAC ACTGAGGGCG 
GTTCGTGTGC TGCGGCCACT CAAGCTGGTG TCTGGAATCC CAAGTTTACA AGTCGTCCTG 
AAGTCAATCA TGAAGGCGAT GATCCCTCTG CTGCAGATCG GCCTCCTCCT GTTTTTTGCA 
ATCCTTATTT TTGCAATCAT AGGGTTAGAA TTTTATATGG GAAAATTTCA TACCACCTGC 
TTTGAAGAGG GGACAGACGA CATCCAGGGT GAGTCGCCAG CTCCGTGTGG GACAGAGGAG 
CCTGCCCGCA CCTGCCCCAA CGGGACCAAA TGTCAGCCGT ACTGGGAAGG GCCCAACAAC 
GGCATCACTC AGTTCGACAA CATCCTGTTT GCTGTGCTCA CTGTTTTCCA GTGCATCACC 
ATGGAAGGCT GGACTGATCT CCTCTACAAT AGCAACGATG CCTCAGGGAA CACTTGGAAC 
TGGTTGTACT TCATCCCCCT CATCATCATC GGCTCCTTTT TTATGCTGAA CCTTGTGCTG 
GGTGTGCTGT CTGGGGAGTT TGCCAAAGAA AGGGAACGTG TAGAGAACCG AAGGGCTTTT 
CTGAAGCTCA GAAGACAACA GCAGATTGAA CGTGAGCTCA ATGGATACAT GGAGTGGATC 
TCGAAAGCAG AGGTGATCCT CGCGGAGGAC GAGACAGACG TGGAGCAGAG GCACCCTTTT 
GATGGAGCTC TTCGGAGAGC TACTCTGAAG AAAAGCAAGA CGGACCTGCT CAACCCTGAG 
GAGGCGGAGG ACCAGCTTGC TGACATCGCG TCTGTGGGGT CTCCCTTCGC CAGAGCCAGC 
ATCAAAAGTG CCAAGCTGGA GAATTCGACT TTTTTCCACA AAAAGGAGAG AAGAATGCGT 
TTCTACATCC GCCGCATGGT CAAAACTCAG GCCTTCTACT GGACCGTGCT CAGTCTGGTA 
GCCCTCAACA CGCTGTGCGT CGCCATTGTC CACTACAACC AGCCCGAGTG GCTCTCCGAC 
TTCCTCTACT ATGCAGAATT CATTTTCTTA GGACTCTTTA TGTCCGAAAT GTTTATAAAA 
ATGTATGGGC TCGGGACACG GCCTTACTTC CACTCTTCCT TCAACTGCTT TGACTGTGGG 
GTCATCATCG GGAGCATCTT TGAAGTCATC TGGGCCGTCA TCAAACCGGG TACATCCTTT 
GGAATCAGCG TGTTACGAGC TCTCAGGTTA CTGCGTATTT TCAAAGTCAC AAAGTACTGG 
GCATCTCTCA GAAACCTGGT TGTCTCCCTC CTCAACTCCA TGAAATCCAT CATAAGTCTG 
CTGTTCCTCC TCTTCCTCTT CATTGTCGTC TTTGCCCTCT TGGGGATGCA GCTGTTTGGT 
GGCCAGTTTA ATTTTGACGA GGGGACTCCT CCCACCAACT TCGACACTTT TCCAGCAGCA 
ATAATGACTG TGTTTCAGAT CCTGACTGGC GAGGATTGGA ATGAGGTCAT GTATGATGGG 
ATCAAGTCTC AGGGGGGCGT GCAGGGCGGC ATGGTGTTCT CCATCTACTT CATCGTCCTC 
ACCCTCTTCG GGAACTACAC CCTGCTGAAC GTGTTCTTGG CTATCGCGGT GGACAACCTG 
GCCAACGCCC AGGAACTCAC CAAGGATGAA CAAGAAGAGG AAGAGGCAGC CAATCAGAAA 
CTGGCTCTAC AGAAAGCCAA GGAGGTGGCA GAAGTGAGTC CCCTGTCTGC AGCCAACATG 
TCCATAGCTG TGAAGGAACA GCAGAAGAAC CAGAAGCCTG CCAAGTCGGT GTGGGAGCAG 
CGCACCAGCG AGATGCGCAA GCAGAACCTG CTGGCTAGCC GCGAGGCGCT GTACGGGGAC 
GCGGCTGAGC GCTGGCCCAC CACTTACGCG CGCCCGCTGC GGCCGGACGT GAAGACGCAC 
TTGGACCGGC CGCTCGTGGT GGACCCGCAG GAGAACCGTA ACAACAACAC CAACAAGAGC 
CGTGCGCCAG AAGCGCTGCG CCAAACCGCG CGGCCCCGCG AGAGCGCGCG CGACCCCGAC 
GCGCGGCGCG CCTGGCCCAG CAGCCCTGAG CGCGCCCCTG GACGAGAGGG CCCGTATGGC 
CGCGAGAGCG AGCCGCAACA GCGCGAGCAC GCGCCACCCC GCGAGCACGT ACCCTGGGAC 
GCGGATCCTG AGCGCGCCAA GGCCGGGGAC GCGCCCCGCC GCCACACGCA CCGGCCTGTG 
GCCGAGGGCG AGCCTCGTCG CCACCGCGCG CGCCGCCGGC CCGGGGACGA ACCGGACGAC 
AGACCGGAGC GCAGGCCGCG TCCCCGCGAC GCCACTAGGC CGGCCCGCGC TGCAGACGGC 
GAAGGCGATG ATGGGGAGCG CAAGCGGCGA CACCGACACG GGCCGCCGGC CCACGATGAC 
AGGGAGCGCA GACACCGGCG CAGAAAAGAG AGCCAGGGCT CTGGGGTCCC CATGTCTGGT 
CCCAACCTGT CCACCACCAG GCCAATCCAG CAGGATCTGG GCCGCCAGGA CCTGCCACTG 
GCTGAGGACC TGGACAACAT GAAGAACAAC AAGTTGGCCA CCGGGGAGCC TGCCAGTCCC 
CACGACAGCC TGGGCCACAG TGGCCTTCCC CCTAGCCCTG CCAAGATCGG GAACAGCACC 
AACCCTGGTC CCGCCTTGGC CACCAATCCC CAGAATGCTG CCAGCCGCAG GACGCCCAAC 
AACCCGGGCA ACCCGTCCAA CCCCGGCCCC CCCAAGACTC CCGAGAACAG CCTTATCGTC 
ACCAACCCCA GCAGCACCCA GCCCAACTCA GCAAAGACTG CCAGGAAACC CGAGCACATG 
GCGGTGGAGA TCCCCCCGGC CTGCCCGCCC CTCAACCACA CTGTGGTCCA AGTAAACAAA 
AACGCCAACC CAGACCCACT GCCAAAGAAA GAGGAAGAGA AGAAGGAGGA AGAGGAGGCA 
GACCCCGGGG AGGATGGCCC AAAGCCCATG CCGCCCTACA GCTCCATGTT CATCCTCTCC 
ACCACCAACC CCCTTCGCCG GCTGTGCCAT TACATCCTGA ACCTGCGCTA CTTCGAGATG 
TGCATCCTCA TGGTCATTGC CATGAGTAGC ATCGCGCTGG CCGCCGAGGA CCCGGTGCAG 
CCCAACGCAC CCCGCAACAA CGTGCTGCGA TATTTTGACT ATGTTTTCAC AGGAGTGTTT 
ACCTTTGAGA TGGTGATCAA GATGATCGAC CTGGGCCTCG TCCTGCATCA GGGGGCCTAT 
TTCCGTGACC TGTGGAACAT TCTGGACTTC ATAGTGGTCA GTGGGGCCCT GGTGGCCTTT 
GCCTTCACGA GCAGTACACG TGGCAATAGC AAAGGAAAGG ACATCAACAC CATCAAGTCC 
CTCCGAGTCC TCCGGGTGCT ACGACCTCTA AAGACCATCA AGCGGCTGCC TAAGTTGAAG 
GCCGTATTTG ACTGCGTGGT GAACTCGCTC AAGAACGTCT TCAACATCCT CATTGTCTAC 
ATGCTCTTCA TGTTCATCTT CGCCGTGGTG GCCGTGCAGC TCTTCAAGGG CAAATTCTTC 
CACTGCACGG ACGAGTCCAA GGAGTTTGAG AGAGACTGTC GAGGCAAATA CCTCCTTTAC 
GAGAAGAACG AGGTAAAGGC GCGGGACCGC GAGTGGAAGA AATACGACTT CCACTACGAC 
AACGTGCTCT GGGCCCTGCT CACGCTCTTT ACGGTGTCCA CGGGAGAGGG CTGGCCACAG 
GTCCTCAAGC ACTCAGTGGA TGCCACTTTT GAGAACCAGG GCCCCAGCCC CGGGTACCGC 
ATGGAAATGT CCATCTTCTA CGTGGTCTAC TTTGTGGTGT TTCCCTTCTT CTTTGTCAAT 
ATCTTTGTGG CCTTGATCAT CATCACCTTC CAGGAGCAGG GAGACAAGAT GATGGAAGAA 
TACAGCCTAG AGAAAAATGA GAGGGCCTGC ATCGACTTTG CCATCAGTGC CAAGCCGCTG 
ACCAGGCACA TGCCCCAGAA CAAGCAGAGC TTCCAGTATC GAATGTGGCA GTTCGTGGTG 
TCCCCACCCT TTGAGTACAC CATCATGGCC ATGATCGCTC TCAACACCAT CGTGCTAATG 
ATGAAGTTCT ATGGAGCCTC TGTGGCCTAT GAAAACGCCC TTCGAGTGTT CAACATTGTC 
TTCACCTCCC TCTTCTCTCT CGAATGTGTG CTCAAAGTCA TGGCTTTTGG GATTCTGAAT 
TATTTCCGCG ATGCCTGGAA CATCTTCGAC TTTGTGACTG TTCTGGGCAG CATCACAGAC 
ATCCTCGTCA CCGAGTTTGG GAATCCGAAT AACTTCATCA ACCTGAGCTT TCTCCGCCTC 
TTCCGTGCTG CCCGACTCAT CAAACTCCTC CGCCAGGGTT ACACCATCCG CATTCTCCTC 
TGGACTTTCG TGCAGTCTTT CAAGGCCCTA CCTTATGTCT GTCTGCTGAT CGCCATGCTC 
TTCTTCATCT ATGCCATCAT CGGGATGCAG GTGATCCCGC ACCCTTGGGG TACCGGGGCT 
GCGGGAGGGA GCTTGTGTCA AAGGGAGCCA AGGTGGCTGT GGCTGCTCCC ATCGTCACCC 
CCCCCCCCAA CTCTCCAGGT GTTTGGCAAC ATCGGCATTG ATGGGGAAGA TGAGGACAGC 
GATGAGGATG AGTTCCAAAT CACGGAGCAC AATAACTTCC GGACCTTCTT CCAAGCTCTC 
ATGCTTCTCT TCCGGAGCGC CACAGGGGAA GCGTGGCACA ACATCATGCT GTCCTGCCTC 
AGCGGGAAGC CATGCGACAA GAACTCCGGG ATCCAAAAAC CAGAGTGTGG CAACGAGTTC 
GCCTATTTTT ACTTTGTCTC GTTCATCTTC CTTTGCTCAT TTCTGATGCT GAATCTCTTT 
GTTGCTGTCA TCATGGACAA CTTCGAGTAC CTCACCCGAG ATTCCTCCAT CCTGGGCCCC 
CACCACCTGG ATGAGTACGT GCGTGTCTGG GCAGAGTATG ACCCTGCTGC CTGCGGCCGC 
ATTCACTATA AGGACATGTA CAGTTTATTG CGAGTAATAT CGCCCCCTCT CGGCTTAGGC 
AAGAAATGTC CTCATAGGGT TGCTTGCAAG AGGCTCTTGC GGATGGACCT ACCCGTAGCG 
GATGACAACA CCGTTCACTT CAACTCCACC TTGATGGCTC TGATCCGAAC CGCCCTGGAT 
ATCAAAATCG CAAAGGGTGG AGCTGACAAG CAGCAAATGG ACGCAGAGCT CCGCAAGGAA 
ATGATGGCCA TTTGGCCCAA CCTGTCTCAG AAGACCTTGG ATCTGCTGGT CACACCTCAC 
AAGTCCACGG ACCTGACAGT GGGTAAGATC TACGCAGCCA TGATGATCAT GGAGTACTAC 
CGGCAGAGCA AGGCCAAGAA GCTGCAGGCC ATGCGAGAGG AGCAGAACCG GACACCACTC 
ATGTTCCAGC GCATGGAGCC TCCATCGCCA ACACAGGAGG GAGGACCCAG CCAAAACGCC 
CTTCCCTCCA CTCAGCTGGA CCCCGGAGGA GGCCTGATGG CTCAAGAAAG CAGCATGAAG 
GAGAGCCCGT CCTGGGTGAC CCAGCGGGCA CAGGAGATGT TCCAGAAGAC TGGTACCTGG 
AGCCCAGAGC GAGGGCCACC CATCGACATG CCTAACAGCC AGCCCAACTC CCAGTCTGTG 
GAGATGAGAG AAATGGGAAC TGATGGCTAC TCAGACAGCG AACACTACCT CCCCATGGAA 
GGACAGACCA GGGCCGCCTC CATGCCCCGC CTCCCAGCAG AGAACCAGAG GAGAAGGGGC 
CGGCCACGTG GAAATAACCT CAGTACCATC TCTGATACCA GCCCCATGAA GCGCTCAGCC 
TCCGTGCTGG GACCCAAAGC CCGGCGACTG GATGACTACT CACTAGAGCG GGTACCACCT 
GAGGAGAACC AAAGGTACCA CCAACGCCGC CGGGACCGTG GCCACCGCAC CTCTGAGCGC 
TCTCTGGGCC GATACACTGA TGTGGACACA GGCCTGGGGA CAGATCTGAG CATGACCACC 
CAATCGGGTG ACCTGCCCTC CAAAGATCGG GACCAGGACC GGGGCCGGCC CAAGGACCGG 
AAGCATCGGC CACACCACCA CCACCACCAT CATCACCATC ATCCCCCGGC CCCGGACCGG 
GAGCGCTACG CACAGGAGCG GCCGGACACC GGCCGGGCGC GGGCCCGGGA GCAGCGCTGG 
TCCCGCTCGC CCAGCGAGGG TCGGGAGCAC GCGACACACA GACAGGGCAG TAGTTCCGTC 
AGTGGAAGCC CAGCCCCCTC TACGTCCGGC ACCAGCACGC CGCGGCGCGG CCGCCGCCAG 
CTCCCCCAGA CCCCCTGCAC CCCGCGGCCG CTTGTGTCCT ACTCGCCCGC TCCGCGCAGG 
CCTGCGGCGC GCAGGATGGC GGGTCCCCCA GCGCCCCCTG GCGGCTCGCC GCGGGGTTGC 
CGCCGTGCGC CGCGCTGGCC TGCGCATGCG CCTGAGGGCC CGCGGCCGCG CGGTGCCGAC 
TACACCGAGC CCGACAGCCC GCGCGAGCCG CCAGGGGGCG CGCATGAGCC CGCGCCGCGG 
TCGCCCAGGA CTCCGCGCGC CGCGGGCTGC GCGTCACCGC GCCACGGCCG CAGGCTGCCC 
AATGGTTACT ACGCGGGGCA CGGCGCGCCA CGGCCGCGCA CAGCCCGCAG GGGCGCGCAC 
GACGCGTACA GCGAGAGCGA GGACGACTGG TGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1a ( XM_008772350.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008770572.2 Rattus norvegicus calcium voltage-gated channel subunit alpha1 A (Cacna1a), transcript variant X4, mRNA.

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CloneID	ORa59367
Clone ID Related Accession (Same CDS sequence)	XM_008772350.2
Accession Version	XM_008772350.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7533bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008772350.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTCCT CTCCTGGGAT TTCTGAGCGA GGCCCATGGA TGCCGAGGGC CTCCTGGGTC 
TCTGACCCCA TGCGTTCTCC CCTCAATCAT GAGCTCCTGC ACAGCTCGAG GTACCCAGGC 
ACGGACTGCA CCCTGGCAGG TTCTTCCAGG ACTTTGCATG TCCCGTGGTC CGAGCAGATG 
AGCGGAAGCC TCGAGGGGCC AACTCTGAAA CGGCGTGGTG GCGTGGTGGA CCACCGGGAT 
GTCATCTTGG CCCACCAGGC GCACAAAATG CACAGCACCC CTCAGGCCAG GAGGAAAGAG 
TGGGAAATGG CCCGCTTTGG AGACGAGATG CCGGGCCGCT ACGGCGCAGG CGGAGGAGGC 
TCAGGGCCGG CCGCCGGGGT GGTCGTGGGC GCCGCGGGCG GCCGAGGAGC CGGGGGCAGC 
CGGCAGGGCG GGCAGCCCGG AGCGCAGAGG ATGTACAAGC AGTCGATGGC GCAGAGAGCG 
CGGACCATGG CCCTCTACAA CCCCATCCCT GTCCGCCAGA ACTGCCTCAC GGTCAACCGC 
TCCCTGTTCC TCTTCAGTGA AGACAACGTG GTGAGAAAAT ACGCCAAAAA GATCACGGAA 
TGGCCTCCCT TCGAGTACAT GATCCTGGCC ACCATCATTG CTAACTGCAT CGTCCTGGCC 
CTGGAGCAGC ACCTCCCTGA TGATGACAAG ACACCCATGT CCGAGCGGCT GGATGACACA 
GAACCCTATT TCATTGGCAT CTTCTGTTTT GAGGCTGGAA TTAAGATCGT GGCTCTTGGC 
TTTGCCTTCC ACAAAGGCTC CTACCTGAGG AATGGCTGGA ACGTCATGGA CTTTGTCGTG 
GTGCTAACAG GCATCTTGGC CACTGTCGGG ACGGAGTTTG ATCTACGGAC ACTGAGGGCG 
GTTCGTGTGC TGCGGCCACT CAAGCTGGTG TCTGGAATCC CAAGTTTACA AGTCGTCCTG 
AAGTCAATCA TGAAGGCGAT GATCCCTCTG CTGCAGATCG GCCTCCTCCT GTTTTTTGCA 
ATCCTTATTT TTGCAATCAT AGGGTTAGAA TTTTATATGG GAAAATTTCA TACCACCTGC 
TTTGAAGAGG GGACAGACGA CATCCAGGGT GAGTCGCCAG CTCCGTGTGG GACAGAGGAG 
CCTGCCCGCA CCTGCCCCAA CGGGACCAAA TGTCAGCCGT ACTGGGAAGG GCCCAACAAC 
GGCATCACTC AGTTCGACAA CATCCTGTTT GCTGTGCTCA CTGTTTTCCA GTGCATCACC 
ATGGAAGGCT GGACTGATCT CCTCTACAAT AGCAACGATG CCTCAGGGAA CACTTGGAAC 
TGGTTGTACT TCATCCCCCT CATCATCATC GGCTCCTTTT TTATGCTGAA CCTTGTGCTG 
GGTGTGCTGT CTGGGGAGTT TGCCAAAGAA AGGGAACGTG TAGAGAACCG AAGGGCTTTT 
CTGAAGCTCA GAAGACAACA GCAGATTGAA CGTGAGCTCA ATGGATACAT GGAGTGGATC 
TCGAAAGCAG AAGAGGTGAT CCTCGCGGAG GACGAGACAG ACGTGGAGCA GAGGCACCCT 
TTTGATGGAG CTCTTCGGAG AGCTACTCTG AAGAAAAGCA AGACGGACCT GCTCAACCCT 
GAGGAGGCGG AGGACCAGCT TGCTGACATC GCGTCTGTGG GGTCTCCCTT CGCCAGAGCC 
AGCATCAAAA GTGCCAAGCT GGAGAATTCG ACTTTTTTCC ACAAAAAGGA GAGAAGAATG 
CGTTTCTACA TCCGCCGCAT GGTCAAAACT CAGGCCTTCT ACTGGACCGT GCTCAGTCTG 
GTAGCCCTCA ACACGCTGTG CGTCGCCATT GTCCACTACA ACCAGCCCGA GTGGCTCTCC 
GACTTCCTCT ACTATGCAGA ATTCATTTTC TTAGGACTCT TTATGTCCGA AATGTTTATA 
AAAATGTATG GGCTCGGGAC ACGGCCTTAC TTCCACTCTT CCTTCAACTG CTTTGACTGT 
GGGGTCATCA TCGGGAGCAT CTTTGAAGTC ATCTGGGCCG TCATCAAACC GGGTACATCC 
TTTGGAATCA GCGTGTTACG AGCTCTCAGG TTACTGCGTA TTTTCAAAGT CACAAAGTAC 
TGGGCATCTC TCAGAAACCT GGTTGTCTCC CTCCTCAACT CCATGAAATC CATCATAAGT 
CTGCTGTTCC TCCTCTTCCT CTTCATTGTC GTCTTTGCCC TCTTGGGGAT GCAGCTGTTT 
GGTGGCCAGT TTAATTTTGA CGAGGGGACT CCTCCCACCA ACTTCGACAC TTTTCCAGCA 
GCAATAATGA CTGTGTTTCA GATCCTGACT GGCGAGGATT GGAATGAGGT CATGTATGAT 
GGGATCAAGT CTCAGGGGGG CGTGCAGGGC GGCATGGTGT TCTCCATCTA CTTCATCGTC 
CTCACCCTCT TCGGGAACTA CACCCTGCTG AACGTGTTCT TGGCTATCGC GGTGGACAAC 
CTGGCCAACG CCCAGGAACT CACCAAGGAT GAACAAGAAG AGGAAGAGGC AGCCAATCAG 
AAACTGGCTC TACAGAAAGC CAAGGAGGTG GCAGAAGTGA GTCCCCTGTC TGCAGCCAAC 
ATGTCCATAG CTGTGAAGGA ACAGCAGAAG AACCAGAAGC CTGCCAAGTC GGTGTGGGAG 
CAGCGCACCA GCGAGATGCG CAAGCAGAAC CTGCTGGCTA GCCGCGAGGC GCTGTACGGG 
GACGCGGCTG AGCGCTGGCC CACCACTTAC GCGCGCCCGC TGCGGCCGGA CGTGAAGACG 
CACTTGGACC GGCCGCTCGT GGTGGACCCG CAGGAGAACC GTAACAACAA CACCAACAAG 
AGCCGTGCGC CAGAAGCGCT GCGCCAAACC GCGCGGCCCC GCGAGAGCGC GCGCGACCCC 
GACGCGCGGC GCGCCTGGCC CAGCAGCCCT GAGCGCGCCC CTGGACGAGA GGGCCCGTAT 
GGCCGCGAGA GCGAGCCGCA ACAGCGCGAG CACGCGCCAC CCCGCGAGCA CGTACCCTGG 
GACGCGGATC CTGAGCGCGC CAAGGCCGGG GACGCGCCCC GCCGCCACAC GCACCGGCCT 
GTGGCCGAGG GCGAGCCTCG TCGCCACCGC GCGCGCCGCC GGCCCGGGGA CGAACCGGAC 
GACAGACCGG AGCGCAGGCC GCGTCCCCGC GACGCCACTA GGCCGGCCCG CGCTGCAGAC 
GGCGAAGGCG ATGATGGGGA GCGCAAGCGG CGACACCGAC ACGGGCCGCC GGCCCACGAT 
GACAGGGAGC GCAGACACCG GCGCAGAAAA GAGAGCCAGG GCTCTGGGGT CCCCATGTCT 
GGTCCCAACC TGTCCACCAC CAGGCCAATC CAGCAGGATC TGGGCCGCCA GGACCTGCCA 
CTGGCTGAGG ACCTGGACAA CATGAAGAAC AACAAGTTGG CCACCGGGGA GCCTGCCAGT 
CCCCACGACA GCCTGGGCCA CAGTGGCCTT CCCCCTAGCC CTGCCAAGAT CGGGAACAGC 
ACCAACCCTG GTCCCGCCTT GGCCACCAAT CCCCAGAATG CTGCCAGCCG CAGGACGCCC 
AACAACCCGG GCAACCCGTC CAACCCCGGC CCCCCCAAGA CTCCCGAGAA CAGCCTTATC 
GTCACCAACC CCAGCAGCAC CCAGCCCAAC TCAGCAAAGA CTGCCAGGAA ACCCGAGCAC 
ATGGCGGTGG AGATCCCCCC GGCCTGCCCG CCCCTCAACC ACACTGTGGT CCAAGTAAAC 
AAAAACGCCA ACCCAGACCC ACTGCCAAAG AAAGAGGAAG AGAAGAAGGA GGAAGAGGAG 
GCAGACCCCG GGGAGGATGG CCCAAAGCCC ATGCCGCCCT ACAGCTCCAT GTTCATCCTC 
TCCACCACCA ACCCCCTTCG CCGGCTGTGC CATTACATCC TGAACCTGCG CTACTTCGAG 
ATGTGCATCC TCATGGTCAT TGCCATGAGT AGCATCGCGC TGGCCGCCGA GGACCCGGTG 
CAGCCCAACG CACCCCGCAA CAACGTGCTG CGATATTTTG ACTATGTTTT CACAGGAGTG 
TTTACCTTTG AGATGGTGAT CAAGATGATC GACCTGGGCC TCGTCCTGCA TCAGGGGGCC 
TATTTCCGTG ACCTGTGGAA CATTCTGGAC TTCATAGTGG TCAGTGGGGC CCTGGTGGCC 
TTTGCCTTCA CGAGCAGTAC ACGTGGCAAT AGCAAAGGAA AGGACATCAA CACCATCAAG 
TCCCTCCGAG TCCTCCGGGT GCTACGACCT CTAAAGACCA TCAAGCGGCT GCCTAAGTTG 
AAGGCCGTAT TTGACTGCGT GGTGAACTCG CTCAAGAACG TCTTCAACAT CCTCATTGTC 
TACATGCTCT TCATGTTCAT CTTCGCCGTG GTGGCCGTGC AGCTCTTCAA GGGCAAATTC 
TTCCACTGCA CGGACGAGTC CAAGGAGTTT GAGAGAGACT GTCGAGGCAA ATACCTCCTT 
TACGAGAAGA ACGAGGTAAA GGCGCGGGAC CGCGAGTGGA AGAAATACGA CTTCCACTAC 
GACAACGTGC TCTGGGCCCT GCTCACGCTC TTTACGGTGT CCACGGGAGA GGGCTGGCCA 
CAGGTCCTCA AGCACTCAGT GGATGCCACT TTTGAGAACC AGGGCCCCAG CCCCGGGTAC 
CGCATGGAAA TGTCCATCTT CTACGTGGTC TACTTTGTGG TGTTTCCCTT CTTCTTTGTC 
AATATCTTTG TGGCCTTGAT CATCATCACC TTCCAGGAGC AGGGAGACAA GATGATGGAA 
GAATACAGCC TAGAGAAAAA TGAGAGGGCC TGCATCGACT TTGCCATCAG TGCCAAGCCG 
CTGACCAGGC ACATGCCCCA GAACAAGCAG AGCTTCCAGT ATCGAATGTG GCAGTTCGTG 
GTGTCCCCAC CCTTTGAGTA CACCATCATG GCCATGATCG CTCTCAACAC CATCGTGCTA 
ATGATGAAGT TCTATGGAGC CTCTGTGGCC TATGAAAACG CCCTTCGAGT GTTCAACATT 
GTCTTCACCT CCCTCTTCTC TCTCGAATGT GTGCTCAAAG TCATGGCTTT TGGGATTCTG 
AATTATTTCC GCGATGCCTG GAACATCTTC GACTTTGTGA CTGTTCTGGG CAGCATCACA 
GACATCCTCG TCACCGAGTT TGGGAATAAC TTCATCAACC TGAGCTTTCT CCGCCTCTTC 
CGTGCTGCCC GACTCATCAA ACTCCTCCGC CAGGGTTACA CCATCCGCAT TCTCCTCTGG 
ACTTTCGTGC AGTCTTTCAA GGCCCTACCT TATGTCTGTC TGCTGATCGC CATGCTCTTC 
TTCATCTATG CCATCATCGG GATGCAGGTG ATCCCGCACC CTTGGGGTAC CGGGGCTGCG 
GGAGGGAGCT TGTGTCAAAG GGAGCCAAGG TGGCTGTGGC TGCTCCCATC GTCACCCCCC 
CCCCCAACTC TCCAGGTGTT TGGCAACATC GGCATTGATG GGGAAGATGA GGACAGCGAT 
GAGGATGAGT TCCAAATCAC GGAGCACAAT AACTTCCGGA CCTTCTTCCA AGCTCTCATG 
CTTCTCTTCC GGAGCGCCAC AGGGGAAGCG TGGCACAACA TCATGCTGTC CTGCCTCAGC 
GGGAAGCCAT GCGACAAGAA CTCCGGGATC CAAAAACCAG AGTGTGGCAA CGAGTTCGCC 
TATTTTTACT TTGTCTCGTT CATCTTCCTT TGCTCATTTC TGATGCTGAA TCTCTTTGTT 
GCTGTCATCA TGGACAACTT CGAGTACCTC ACCCGAGATT CCTCCATCCT GGGCCCCCAC 
CACCTGGATG AGTACGTGCG TGTCTGGGCA GAGTATGACC CTGCTGCCTG CGGCCGCATT 
CACTATAAGG ACATGTACAG TTTATTGCGA GTAATATCGC CCCCTCTCGG CTTAGGCAAG 
AAATGTCCTC ATAGGGTTGC TTGCAAGAGG CTCTTGCGGA TGGACCTACC CGTAGCGGAT 
GACAACACCG TTCACTTCAA CTCCACCTTG ATGGCTCTGA TCCGAACCGC CCTGGATATC 
AAAATCGCAA AGGGTGGAGC TGACAAGCAG CAAATGGACG CAGAGCTCCG CAAGGAAATG 
ATGGCCATTT GGCCCAACCT GTCTCAGAAG ACCTTGGATC TGCTGGTCAC ACCTCACAAG 
TCCACGGACC TGACAGTGGG TAAGATCTAC GCAGCCATGA TGATCATGGA GTACTACCGG 
CAGAGCAAGG CCAAGAAGCT GCAGGCCATG CGAGAGGAGC AGAACCGGAC ACCACTCATG 
TTCCAGCGCA TGGAGCCTCC ATCGCCAACA CAGGAGGGAG GACCCAGCCA AAACGCCCTT 
CCCTCCACTC AGCTGGACCC CGGAGGAGGC CTGATGGCTC AAGAAAGCAG CATGAAGGAG 
AGCCCGTCCT GGGTGACCCA GCGGGCACAG GAGATGTTCC AGAAGACTGG TACCTGGAGC 
CCAGAGCGAG GGCCACCCAT CGACATGCCT AACAGCCAGC CCAACTCCCA GTCTGTGGAG 
ATGAGAGAAA TGGGAACTGA TGGCTACTCA GACAGCGAAC ACTACCTCCC CATGGAAGGA 
CAGACCAGGG CCGCCTCCAT GCCCCGCCTC CCAGCAGAGA ACCAGAGGAG AAGGGGCCGG 
CCACGTGGAA ATAACCTCAG TACCATCTCT GATACCAGCC CCATGAAGCG CTCAGCCTCC 
GTGCTGGGAC CCAAAGCCCG GCGACTGGAT GACTACTCAC TAGAGCGGGT ACCACCTGAG 
GAGAACCAAA GGTACCACCA ACGCCGCCGG GACCGTGGCC ACCGCACCTC TGAGCGCTCT 
CTGGGCCGAT ACACTGATGT GGACACAGGC CTGGGGACAG ATCTGAGCAT GACCACCCAA 
TCGGGTGACC TGCCCTCCAA AGATCGGGAC CAGGACCGGG GCCGGCCCAA GGACCGGAAG 
CATCGGCCAC ACCACCACCA CCACCATCAT CACCATCATC CCCCGGCCCC GGACCGGGAG 
CGCTACGCAC AGGAGCGGCC GGACACCGGC CGGGCGCGGG CCCGGGAGCA GCGCTGGTCC 
CGCTCGCCCA GCGAGGGTCG GGAGCACGCG ACACACAGAC AGGGCAGTAG TTCCGTCAGT 
GGAAGCCCAG CCCCCTCTAC GTCCGGCACC AGCACGCCGC GGCGCGGCCG CCGCCAGCTC 
CCCCAGACCC CCTGCACCCC GCGGCCGCTT GTGTCCTACT CGCCCGCTCC GCGCAGGCCT 
GCGGCGCGCA GGATGGCGGG TCCCCCAGCG CCCCCTGGCG GCTCGCCGCG GGGTTGCCGC 
CGTGCGCCGC GCTGGCCTGC GCATGCGCCT GAGGGCCCGC GGCCGCGCGG TGCCGACTAC 
ACCGAGCCCG ACAGCCCGCG CGAGCCGCCA GGGGGCGCGC ATGAGCCCGC GCCGCGGTCG 
CCCAGGACTC CGCGCGCCGC GGGCTGCGCG TCACCGCGCC ACGGCCGCAG GCTGCCCAAT 
GGTTACTACG CGGGGCACGG CGCGCCACGG CCGCGCACAG CCCGCAGGGG CGCGCACGAC 
GCGTACAGCG AGAGCGAGGA CGACTGGTGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008770572.2
CDS	107..7639
Translation	MVSSPGISERGPWMPRASWVSDPMRSPLNHELLHSSRYPGTDCTLAGSSRTLHVPWSEQMSGSLEGPTLKRRGGVVDHRDVILAHQAHKMHSTPQARRKEWEMARFGDEMPGRYGAGGGGSGPAAGVVVGAAGGRGAGGSRQGGQPGAQRMYKQSMAQRARTMALYNPIPVRQNCLTVNRSLFLFSEDNVVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDDDKTPMSERLDDTEPYFIGIFCFEAGIKIVALGFAFHKGSYLRNGWNVMDFVVVLTGILATVGTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILIFAIIGLEFYMGKFHTTCFEEGTDDIQGESPAPCGTEEPARTCPNGTKCQPYWEGPNNGITQFDNILFAVLTVFQCITMEGWTDLLYNSNDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYMEWISKAEEVILAEDETDVEQRHPFDGALRRATLKKSKTDLLNPEEAEDQLADIASVGSPFARASIKSAKLENSTFFHKKERRMRFYIRRMVKTQAFYWTVLSLVALNTLCVAIVHYNQPEWLSDFLYYAEFIFLGLFMSEMFIKMYGLGTRPYFHSSFNCFDCGVIIGSIFEVIWAVIKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFDEGTPPTNFDTFPAAIMTVFQILTGEDWNEVMYDGIKSQGGVQGGMVFSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAANQKLALQKAKEVAEVSPLSAANMSIAVKEQQKNQKPAKSVWEQRTSEMRKQNLLASREALYGDAAERWPTTYARPLRPDVKTHLDRPLVVDPQENRNNNTNKSRAPEALRQTARPRESARDPDARRAWPSSPERAPGREGPYGRESEPQQREHAPPREHVPWDADPERAKAGDAPRRHTHRPVAEGEPRRHRARRRPGDEPDDRPERRPRPRDATRPARAADGEGDDGERKRRHRHGPPAHDDRERRHRRRKESQGSGVPMSGPNLSTTRPIQQDLGRQDLPLAEDLDNMKNNKLATGEPASPHDSLGHSGLPPSPAKIGNSTNPGPALATNPQNAASRRTPNNPGNPSNPGPPKTPENSLIVTNPSSTQPNSAKTARKPEHMAVEIPPACPPLNHTVVQVNKNANPDPLPKKEEEKKEEEEADPGEDGPKPMPPYSSMFILSTTNPLRRLCHYILNLRYFEMCILMVIAMSSIALAAEDPVQPNAPRNNVLRYFDYVFTGVFTFEMVIKMIDLGLVLHQGAYFRDLWNILDFIVVSGALVAFAFTSSTRGNSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYMLFMFIFAVVAVQLFKGKFFHCTDESKEFERDCRGKYLLYEKNEVKARDREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATFENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEEYSLEKNERACIDFAISAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYTIMAMIALNTIVLMMKFYGASVAYENALRVFNIVFTSLFSLECVLKVMAFGILNYFRDAWNIFDFVTVLGSITDILVTEFGNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVIPHPWGTGAAGGSLCQREPRWLWLLPSSPPPPTLQVFGNIGIDGEDEDSDEDEFQITEHNNFRTFFQALMLLFRSATGEAWHNIMLSCLSGKPCDKNSGIQKPECGNEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRVWAEYDPAACGRIHYKDMYSLLRVISPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKQQMDAELRKEMMAIWPNLSQKTLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKKLQAMREEQNRTPLMFQRMEPPSPTQEGGPSQNALPSTQLDPGGGLMAQESSMKESPSWVTQRAQEMFQKTGTWSPERGPPIDMPNSQPNSQSVEMREMGTDGYSDSEHYLPMEGQTRAASMPRLPAENQRRRGRPRGNNLSTISDTSPMKRSASVLGPKARRLDDYSLERVPPEENQRYHQRRRDRGHRTSERSLGRYTDVDTGLGTDLSMTTQSGDLPSKDRDQDRGRPKDRKHRPHHHHHHHHHHPPAPDRERYAQERPDTGRARAREQRWSRSPSEGREHATHRQGSSSVSGSPAPSTSGTSTPRRGRRQLPQTPCTPRPLVSYSPAPRRPAARRMAGPPAPPGGSPRGCRRAPRWPAHAPEGPRPRGADYTEPDSPREPPGGAHEPAPRSPRTPRAAGCASPRHGRRLPNGYYAGHGAPRPRTARRGAHDAYSESEDDWC

Target ORF information:

RefSeq Version	XM_008772350.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel subunit alpha1 A (Cacna1a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772350.2

ORF Insert Sequence:

ATGGTTTCCT CTCCTGGGAT TTCTGAGCGA GGCCCATGGA TGCCGAGGGC CTCCTGGGTC 
TCTGACCCCA TGCGTTCTCC CCTCAATCAT GAGCTCCTGC ACAGCTCGAG GTACCCAGGC 
ACGGACTGCA CCCTGGCAGG TTCTTCCAGG ACTTTGCATG TCCCGTGGTC CGAGCAGATG 
AGCGGAAGCC TCGAGGGGCC AACTCTGAAA CGGCGTGGTG GCGTGGTGGA CCACCGGGAT 
GTCATCTTGG CCCACCAGGC GCACAAAATG CACAGCACCC CTCAGGCCAG GAGGAAAGAG 
TGGGAAATGG CCCGCTTTGG AGACGAGATG CCGGGCCGCT ACGGCGCAGG CGGAGGAGGC 
TCAGGGCCGG CCGCCGGGGT GGTCGTGGGC GCCGCGGGCG GCCGAGGAGC CGGGGGCAGC 
CGGCAGGGCG GGCAGCCCGG AGCGCAGAGG ATGTACAAGC AGTCGATGGC GCAGAGAGCG 
CGGACCATGG CCCTCTACAA CCCCATCCCT GTCCGCCAGA ACTGCCTCAC GGTCAACCGC 
TCCCTGTTCC TCTTCAGTGA AGACAACGTG GTGAGAAAAT ACGCCAAAAA GATCACGGAA 
TGGCCTCCCT TCGAGTACAT GATCCTGGCC ACCATCATTG CTAACTGCAT CGTCCTGGCC 
CTGGAGCAGC ACCTCCCTGA TGATGACAAG ACACCCATGT CCGAGCGGCT GGATGACACA 
GAACCCTATT TCATTGGCAT CTTCTGTTTT GAGGCTGGAA TTAAGATCGT GGCTCTTGGC 
TTTGCCTTCC ACAAAGGCTC CTACCTGAGG AATGGCTGGA ACGTCATGGA CTTTGTCGTG 
GTGCTAACAG GCATCTTGGC CACTGTCGGG ACGGAGTTTG ATCTACGGAC ACTGAGGGCG 
GTTCGTGTGC TGCGGCCACT CAAGCTGGTG TCTGGAATCC CAAGTTTACA AGTCGTCCTG 
AAGTCAATCA TGAAGGCGAT GATCCCTCTG CTGCAGATCG GCCTCCTCCT GTTTTTTGCA 
ATCCTTATTT TTGCAATCAT AGGGTTAGAA TTTTATATGG GAAAATTTCA TACCACCTGC 
TTTGAAGAGG GGACAGACGA CATCCAGGGT GAGTCGCCAG CTCCGTGTGG GACAGAGGAG 
CCTGCCCGCA CCTGCCCCAA CGGGACCAAA TGTCAGCCGT ACTGGGAAGG GCCCAACAAC 
GGCATCACTC AGTTCGACAA CATCCTGTTT GCTGTGCTCA CTGTTTTCCA GTGCATCACC 
ATGGAAGGCT GGACTGATCT CCTCTACAAT AGCAACGATG CCTCAGGGAA CACTTGGAAC 
TGGTTGTACT TCATCCCCCT CATCATCATC GGCTCCTTTT TTATGCTGAA CCTTGTGCTG 
GGTGTGCTGT CTGGGGAGTT TGCCAAAGAA AGGGAACGTG TAGAGAACCG AAGGGCTTTT 
CTGAAGCTCA GAAGACAACA GCAGATTGAA CGTGAGCTCA ATGGATACAT GGAGTGGATC 
TCGAAAGCAG AAGAGGTGAT CCTCGCGGAG GACGAGACAG ACGTGGAGCA GAGGCACCCT 
TTTGATGGAG CTCTTCGGAG AGCTACTCTG AAGAAAAGCA AGACGGACCT GCTCAACCCT 
GAGGAGGCGG AGGACCAGCT TGCTGACATC GCGTCTGTGG GGTCTCCCTT CGCCAGAGCC 
AGCATCAAAA GTGCCAAGCT GGAGAATTCG ACTTTTTTCC ACAAAAAGGA GAGAAGAATG 
CGTTTCTACA TCCGCCGCAT GGTCAAAACT CAGGCCTTCT ACTGGACCGT GCTCAGTCTG 
GTAGCCCTCA ACACGCTGTG CGTCGCCATT GTCCACTACA ACCAGCCCGA GTGGCTCTCC 
GACTTCCTCT ACTATGCAGA ATTCATTTTC TTAGGACTCT TTATGTCCGA AATGTTTATA 
AAAATGTATG GGCTCGGGAC ACGGCCTTAC TTCCACTCTT CCTTCAACTG CTTTGACTGT 
GGGGTCATCA TCGGGAGCAT CTTTGAAGTC ATCTGGGCCG TCATCAAACC GGGTACATCC 
TTTGGAATCA GCGTGTTACG AGCTCTCAGG TTACTGCGTA TTTTCAAAGT CACAAAGTAC 
TGGGCATCTC TCAGAAACCT GGTTGTCTCC CTCCTCAACT CCATGAAATC CATCATAAGT 
CTGCTGTTCC TCCTCTTCCT CTTCATTGTC GTCTTTGCCC TCTTGGGGAT GCAGCTGTTT 
GGTGGCCAGT TTAATTTTGA CGAGGGGACT CCTCCCACCA ACTTCGACAC TTTTCCAGCA 
GCAATAATGA CTGTGTTTCA GATCCTGACT GGCGAGGATT GGAATGAGGT CATGTATGAT 
GGGATCAAGT CTCAGGGGGG CGTGCAGGGC GGCATGGTGT TCTCCATCTA CTTCATCGTC 
CTCACCCTCT TCGGGAACTA CACCCTGCTG AACGTGTTCT TGGCTATCGC GGTGGACAAC 
CTGGCCAACG CCCAGGAACT CACCAAGGAT GAACAAGAAG AGGAAGAGGC AGCCAATCAG 
AAACTGGCTC TACAGAAAGC CAAGGAGGTG GCAGAAGTGA GTCCCCTGTC TGCAGCCAAC 
ATGTCCATAG CTGTGAAGGA ACAGCAGAAG AACCAGAAGC CTGCCAAGTC GGTGTGGGAG 
CAGCGCACCA GCGAGATGCG CAAGCAGAAC CTGCTGGCTA GCCGCGAGGC GCTGTACGGG 
GACGCGGCTG AGCGCTGGCC CACCACTTAC GCGCGCCCGC TGCGGCCGGA CGTGAAGACG 
CACTTGGACC GGCCGCTCGT GGTGGACCCG CAGGAGAACC GTAACAACAA CACCAACAAG 
AGCCGTGCGC CAGAAGCGCT GCGCCAAACC GCGCGGCCCC GCGAGAGCGC GCGCGACCCC 
GACGCGCGGC GCGCCTGGCC CAGCAGCCCT GAGCGCGCCC CTGGACGAGA GGGCCCGTAT 
GGCCGCGAGA GCGAGCCGCA ACAGCGCGAG CACGCGCCAC CCCGCGAGCA CGTACCCTGG 
GACGCGGATC CTGAGCGCGC CAAGGCCGGG GACGCGCCCC GCCGCCACAC GCACCGGCCT 
GTGGCCGAGG GCGAGCCTCG TCGCCACCGC GCGCGCCGCC GGCCCGGGGA CGAACCGGAC 
GACAGACCGG AGCGCAGGCC GCGTCCCCGC GACGCCACTA GGCCGGCCCG CGCTGCAGAC 
GGCGAAGGCG ATGATGGGGA GCGCAAGCGG CGACACCGAC ACGGGCCGCC GGCCCACGAT 
GACAGGGAGC GCAGACACCG GCGCAGAAAA GAGAGCCAGG GCTCTGGGGT CCCCATGTCT 
GGTCCCAACC TGTCCACCAC CAGGCCAATC CAGCAGGATC TGGGCCGCCA GGACCTGCCA 
CTGGCTGAGG ACCTGGACAA CATGAAGAAC AACAAGTTGG CCACCGGGGA GCCTGCCAGT 
CCCCACGACA GCCTGGGCCA CAGTGGCCTT CCCCCTAGCC CTGCCAAGAT CGGGAACAGC 
ACCAACCCTG GTCCCGCCTT GGCCACCAAT CCCCAGAATG CTGCCAGCCG CAGGACGCCC 
AACAACCCGG GCAACCCGTC CAACCCCGGC CCCCCCAAGA CTCCCGAGAA CAGCCTTATC 
GTCACCAACC CCAGCAGCAC CCAGCCCAAC TCAGCAAAGA CTGCCAGGAA ACCCGAGCAC 
ATGGCGGTGG AGATCCCCCC GGCCTGCCCG CCCCTCAACC ACACTGTGGT CCAAGTAAAC 
AAAAACGCCA ACCCAGACCC ACTGCCAAAG AAAGAGGAAG AGAAGAAGGA GGAAGAGGAG 
GCAGACCCCG GGGAGGATGG CCCAAAGCCC ATGCCGCCCT ACAGCTCCAT GTTCATCCTC 
TCCACCACCA ACCCCCTTCG CCGGCTGTGC CATTACATCC TGAACCTGCG CTACTTCGAG 
ATGTGCATCC TCATGGTCAT TGCCATGAGT AGCATCGCGC TGGCCGCCGA GGACCCGGTG 
CAGCCCAACG CACCCCGCAA CAACGTGCTG CGATATTTTG ACTATGTTTT CACAGGAGTG 
TTTACCTTTG AGATGGTGAT CAAGATGATC GACCTGGGCC TCGTCCTGCA TCAGGGGGCC 
TATTTCCGTG ACCTGTGGAA CATTCTGGAC TTCATAGTGG TCAGTGGGGC CCTGGTGGCC 
TTTGCCTTCA CGAGCAGTAC ACGTGGCAAT AGCAAAGGAA AGGACATCAA CACCATCAAG 
TCCCTCCGAG TCCTCCGGGT GCTACGACCT CTAAAGACCA TCAAGCGGCT GCCTAAGTTG 
AAGGCCGTAT TTGACTGCGT GGTGAACTCG CTCAAGAACG TCTTCAACAT CCTCATTGTC 
TACATGCTCT TCATGTTCAT CTTCGCCGTG GTGGCCGTGC AGCTCTTCAA GGGCAAATTC 
TTCCACTGCA CGGACGAGTC CAAGGAGTTT GAGAGAGACT GTCGAGGCAA ATACCTCCTT 
TACGAGAAGA ACGAGGTAAA GGCGCGGGAC CGCGAGTGGA AGAAATACGA CTTCCACTAC 
GACAACGTGC TCTGGGCCCT GCTCACGCTC TTTACGGTGT CCACGGGAGA GGGCTGGCCA 
CAGGTCCTCA AGCACTCAGT GGATGCCACT TTTGAGAACC AGGGCCCCAG CCCCGGGTAC 
CGCATGGAAA TGTCCATCTT CTACGTGGTC TACTTTGTGG TGTTTCCCTT CTTCTTTGTC 
AATATCTTTG TGGCCTTGAT CATCATCACC TTCCAGGAGC AGGGAGACAA GATGATGGAA 
GAATACAGCC TAGAGAAAAA TGAGAGGGCC TGCATCGACT TTGCCATCAG TGCCAAGCCG 
CTGACCAGGC ACATGCCCCA GAACAAGCAG AGCTTCCAGT ATCGAATGTG GCAGTTCGTG 
GTGTCCCCAC CCTTTGAGTA CACCATCATG GCCATGATCG CTCTCAACAC CATCGTGCTA 
ATGATGAAGT TCTATGGAGC CTCTGTGGCC TATGAAAACG CCCTTCGAGT GTTCAACATT 
GTCTTCACCT CCCTCTTCTC TCTCGAATGT GTGCTCAAAG TCATGGCTTT TGGGATTCTG 
AATTATTTCC GCGATGCCTG GAACATCTTC GACTTTGTGA CTGTTCTGGG CAGCATCACA 
GACATCCTCG TCACCGAGTT TGGGAATAAC TTCATCAACC TGAGCTTTCT CCGCCTCTTC 
CGTGCTGCCC GACTCATCAA ACTCCTCCGC CAGGGTTACA CCATCCGCAT TCTCCTCTGG 
ACTTTCGTGC AGTCTTTCAA GGCCCTACCT TATGTCTGTC TGCTGATCGC CATGCTCTTC 
TTCATCTATG CCATCATCGG GATGCAGGTG ATCCCGCACC CTTGGGGTAC CGGGGCTGCG 
GGAGGGAGCT TGTGTCAAAG GGAGCCAAGG TGGCTGTGGC TGCTCCCATC GTCACCCCCC 
CCCCCAACTC TCCAGGTGTT TGGCAACATC GGCATTGATG GGGAAGATGA GGACAGCGAT 
GAGGATGAGT TCCAAATCAC GGAGCACAAT AACTTCCGGA CCTTCTTCCA AGCTCTCATG 
CTTCTCTTCC GGAGCGCCAC AGGGGAAGCG TGGCACAACA TCATGCTGTC CTGCCTCAGC 
GGGAAGCCAT GCGACAAGAA CTCCGGGATC CAAAAACCAG AGTGTGGCAA CGAGTTCGCC 
TATTTTTACT TTGTCTCGTT CATCTTCCTT TGCTCATTTC TGATGCTGAA TCTCTTTGTT 
GCTGTCATCA TGGACAACTT CGAGTACCTC ACCCGAGATT CCTCCATCCT GGGCCCCCAC 
CACCTGGATG AGTACGTGCG TGTCTGGGCA GAGTATGACC CTGCTGCCTG CGGCCGCATT 
CACTATAAGG ACATGTACAG TTTATTGCGA GTAATATCGC CCCCTCTCGG CTTAGGCAAG 
AAATGTCCTC ATAGGGTTGC TTGCAAGAGG CTCTTGCGGA TGGACCTACC CGTAGCGGAT 
GACAACACCG TTCACTTCAA CTCCACCTTG ATGGCTCTGA TCCGAACCGC CCTGGATATC 
AAAATCGCAA AGGGTGGAGC TGACAAGCAG CAAATGGACG CAGAGCTCCG CAAGGAAATG 
ATGGCCATTT GGCCCAACCT GTCTCAGAAG ACCTTGGATC TGCTGGTCAC ACCTCACAAG 
TCCACGGACC TGACAGTGGG TAAGATCTAC GCAGCCATGA TGATCATGGA GTACTACCGG 
CAGAGCAAGG CCAAGAAGCT GCAGGCCATG CGAGAGGAGC AGAACCGGAC ACCACTCATG 
TTCCAGCGCA TGGAGCCTCC ATCGCCAACA CAGGAGGGAG GACCCAGCCA AAACGCCCTT 
CCCTCCACTC AGCTGGACCC CGGAGGAGGC CTGATGGCTC AAGAAAGCAG CATGAAGGAG 
AGCCCGTCCT GGGTGACCCA GCGGGCACAG GAGATGTTCC AGAAGACTGG TACCTGGAGC 
CCAGAGCGAG GGCCACCCAT CGACATGCCT AACAGCCAGC CCAACTCCCA GTCTGTGGAG 
ATGAGAGAAA TGGGAACTGA TGGCTACTCA GACAGCGAAC ACTACCTCCC CATGGAAGGA 
CAGACCAGGG CCGCCTCCAT GCCCCGCCTC CCAGCAGAGA ACCAGAGGAG AAGGGGCCGG 
CCACGTGGAA ATAACCTCAG TACCATCTCT GATACCAGCC CCATGAAGCG CTCAGCCTCC 
GTGCTGGGAC CCAAAGCCCG GCGACTGGAT GACTACTCAC TAGAGCGGGT ACCACCTGAG 
GAGAACCAAA GGTACCACCA ACGCCGCCGG GACCGTGGCC ACCGCACCTC TGAGCGCTCT 
CTGGGCCGAT ACACTGATGT GGACACAGGC CTGGGGACAG ATCTGAGCAT GACCACCCAA 
TCGGGTGACC TGCCCTCCAA AGATCGGGAC CAGGACCGGG GCCGGCCCAA GGACCGGAAG 
CATCGGCCAC ACCACCACCA CCACCATCAT CACCATCATC CCCCGGCCCC GGACCGGGAG 
CGCTACGCAC AGGAGCGGCC GGACACCGGC CGGGCGCGGG CCCGGGAGCA GCGCTGGTCC 
CGCTCGCCCA GCGAGGGTCG GGAGCACGCG ACACACAGAC AGGGCAGTAG TTCCGTCAGT 
GGAAGCCCAG CCCCCTCTAC GTCCGGCACC AGCACGCCGC GGCGCGGCCG CCGCCAGCTC 
CCCCAGACCC CCTGCACCCC GCGGCCGCTT GTGTCCTACT CGCCCGCTCC GCGCAGGCCT 
GCGGCGCGCA GGATGGCGGG TCCCCCAGCG CCCCCTGGCG GCTCGCCGCG GGGTTGCCGC 
CGTGCGCCGC GCTGGCCTGC GCATGCGCCT GAGGGCCCGC GGCCGCGCGG TGCCGACTAC 
ACCGAGCCCG ACAGCCCGCG CGAGCCGCCA GGGGGCGCGC ATGAGCCCGC GCCGCGGTCG 
CCCAGGACTC CGCGCGCCGC GGGCTGCGCG TCACCGCGCC ACGGCCGCAG GCTGCCCAAT 
GGTTACTACG CGGGGCACGG CGCGCCACGG CCGCGCACAG CCCGCAGGGG CGCGCACGAC 
GCGTACAGCG AGAGCGAGGA CGACTGGTGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1a ( XM_017601178.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017456667.1 Rattus norvegicus calcium voltage-gated channel subunit alpha1 A (Cacna1a), transcript variant X8, mRNA.

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CloneID	ORa59371
Clone ID Related Accession (Same CDS sequence)	XM_017601178.1
Accession Version	XM_017601178.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTCCT CTCCTGGGAT TTCTGAGCGA GGCCCATGGA TGCCGAGGGC CTCCTGGGTC 
TCTGACCCCA TGCGTTCTCC CCTCAATCAT GAGCTCCTGC ACAGCTCGAG GTACCCAGGC 
ACGGACTGCA CCCTGGCAGG TTCTTCCAGG ACTTTGCATG TCCCGTGGTC CGAGCAGATG 
AGCGGAAGCC TCGAGGGGCC AACTCTGAAA CGGCGTGGTG GCGTGGTGGA CCACCGGGAT 
GTCATCTTGG CCCACCAGGC GCACAAAATG CACAGCACCC CTCAGGCCAG GAGGAAAGAG 
TGGGAAATGG CCCGCTTTGG AGACGAGATG CCGGGCCGCT ACGGCGCAGG CGGAGGAGGC 
TCAGGGCCGG CCGCCGGGGT GGTCGTGGGC GCCGCGGGCG GCCGAGGAGC CGGGGGCAGC 
CGGCAGGGCG GGCAGCCCGG AGCGCAGAGG ATGTACAAGC AGTCGATGGC GCAGAGAGCG 
CGGACCATGG CCCTCTACAA CCCCATCCCT GTCCGCCAGA ACTGCCTCAC GGTCAACCGC 
TCCCTGTTCC TCTTCAGTGA AGACAACGTG GTGAGAAAAT ACGCCAAAAA GATCACGGAA 
TGGCCTCCCT TCGAGTACAT GATCCTGGCC ACCATCATTG CTAACTGCAT CGTCCTGGCC 
CTGGAGCAGC ACCTCCCTGA TGATGACAAG ACACCCATGT CCGAGCGGCT GGATGACACA 
GAACCCTATT TCATTGGCAT CTTCTGTTTT GAGGCTGGAA TTAAGATCGT GGCTCTTGGC 
TTTGCCTTCC ACAAAGGCTC CTACCTGAGG AATGGCTGGA ACGTCATGGA CTTTGTCGTG 
GTGCTAACAG GCATCTTGGC CACTGTCGGG ACGGAGTTTG ATCTACGGAC ACTGAGGGCG 
GTTCGTGTGC TGCGGCCACT CAAGCTGGTG TCTGGAATCC CAAGTTTACA AGTCGTCCTG 
AAGTCAATCA TGAAGGCGAT GATCCCTCTG CTGCAGATCG GCCTCCTCCT GTTTTTTGCA 
ATCCTTATTT TTGCAATCAT AGGGTTAGAA TTTTATATGG GAAAATTTCA TACCACCTGC 
TTTGAAGAGG GGACAGACGA CATCCAGGGT GAGTCGCCAG CTCCGTGTGG GACAGAGGAG 
CCTGCCCGCA CCTGCCCCAA CGGGACCAAA TGTCAGCCGT ACTGGGAAGG GCCCAACAAC 
GGCATCACTC AGTTCGACAA CATCCTGTTT GCTGTGCTCA CTGTTTTCCA GTGCATCACC 
ATGGAAGGCT GGACTGATCT CCTCTACAAT AGCAACGATG CCTCAGGGAA CACTTGGAAC 
TGGTTGTACT TCATCCCCCT CATCATCATC GGCTCCTTTT TTATGCTGAA CCTTGTGCTG 
GGTGTGCTGT CTGGGGAGTT TGCCAAAGAA AGGGAACGTG TAGAGAACCG AAGGGCTTTT 
CTGAAGCTCA GAAGACAACA GCAGATTGAA CGTGAGCTCA ATGGATACAT GGAGTGGATC 
TCGAAAGCAG AAGAGGTGAT CCTCGCGGAG GACGAGACAG ACGTGGAGCA GAGGCACCCT 
TTTGATGGAG CTCTTCGGAG AGCTACTCTG AAGAAAAGCA AGACGGACCT GCTCAACCCT 
GAGGAGGCGG AGGACCAGCT TGCTGACATC GCGTCTGTGG GGTCTCCCTT CGCCAGAGCC 
AGCATCAAAA GTGCCAAGCT GGAGAATTCG ACTTTTTTCC ACAAAAAGGA GAGAAGAATG 
CGTTTCTACA TCCGCCGCAT GGTCAAAACT CAGGCCTTCT ACTGGACCGT GCTCAGTCTG 
GTAGCCCTCA ACACGCTGTG CGTCGCCATT GTCCACTACA ACCAGCCCGA GTGGCTCTCC 
GACTTCCTCT ACTATGCAGA ATTCATTTTC TTAGGACTCT TTATGTCCGA AATGTTTATA 
AAAATGTATG GGCTCGGGAC ACGGCCTTAC TTCCACTCTT CCTTCAACTG CTTTGACTGT 
GGGGTCATCA TCGGGAGCAT CTTTGAAGTC ATCTGGGCCG TCATCAAACC GGGTACATCC 
TTTGGAATCA GCGTGTTACG AGCTCTCAGG TTACTGCGTA TTTTCAAAGT CACAAAGTAC 
TGGGCATCTC TCAGAAACCT GGTTGTCTCC CTCCTCAACT CCATGAAATC CATCATAAGT 
CTGCTGTTCC TCCTCTTCCT CTTCATTGTC GTCTTTGCCC TCTTGGGGAT GCAGCTGTTT 
GGTGGCCAGT TTAATTTTGA CGAGGGGACT CCTCCCACCA ACTTCGACAC TTTTCCAGCA 
GCAATAATGA CTGTGTTTCA GATCCTGACT GGCGAGGATT GGAATGAGGT CATGTATGAT 
GGGATCAAGT CTCAGGGGGG CGTGCAGGGC GGCATGGTGT TCTCCATCTA CTTCATCGTC 
CTCACCCTCT TCGGGAACTA CACCCTGCTG AACGTGTTCT TGGCTATCGC GGTGGACAAC 
CTGGCCAACG CCCAGGAACT CACCAAGGAT GAACAAGAAG AGGAAGAGGC AGCCAATCAG 
AAACTGGCTC TACAGAAAGC CAAGGAGGTG GCAGAAGTGA GTCCCCTGTC TGCAGCCAAC 
ATGTCCATAG CTGTGAAGGA ACAGCAGAAG AACCAGAAGC CTGCCAAGTC GGTGTGGGAG 
CAGCGCACCA GCGAGATGCG CAAGCAGAAC CTGCTGGCTA GCCGCGAGGC GCTGTACGGG 
GACGCGGCTG AGCGCTGGCC CACCACTTAC GCGCGCCCGC TGCGGCCGGA CGTGAAGACG 
CACTTGGACC GGCCGCTCGT GGTGGACCCG CAGGAGAACC GTAACAACAA CACCAACAAG 
AGCCGTGCGC CAGAAGCGCT GCGCCAAACC GCGCGGCCCC GCGAGAGCGC GCGCGACCCC 
GACGCGCGGC GCGCCTGGCC CAGCAGCCCT GAGCGCGCCC CTGGACGAGA GGGCCCGTAT 
GGCCGCGAGA GCGAGCCGCA ACAGCGCGAG CACGCGCCAC CCCGCGAGCA CGTACCCTGG 
GACGCGGATC CTGAGCGCGC CAAGGCCGGG GACGCGCCCC GCCGCCACAC GCACCGGCCT 
GTGGCCGAGG GCGAGCCTCG TCGCCACCGC GCGCGCCGCC GGCCCGGGGA CGAACCGGAC 
GACAGACCGG AGCGCAGGCC GCGTCCCCGC GACGCCACTA GGCCGGCCCG CGCTGCAGAC 
GGCGAAGGCG ATGATGGGGA GCGCAAGCGG CGACACCGAC ACGGGCCGCC GGCCCACGAT 
GACAGGGAGC GCAGACACCG GCGCAGAAAA GAGAGCCAGG GCTCTGGGGT CCCCATGTCT 
GGTCCCAACC TGTCCACCAC CAGGCCAATC CAGCAGGATC TGGGCCGCCA GGACCTGCCA 
CTGGCTGAGG ACCTGGACAA CATGAAGAAC AACAAGTTGG CCACCGGGGA GCCTGCCAGT 
CCCCACGACA GCCTGGGCCA CAGTGGCCTT CCCCCTAGCC CTGCCAAGAT CGGGAACAGC 
ACCAACCCTG GTCCCGCCTT GGCCACCAAT CCCCAGAATG CTGCCAGCCG CAGGACGCCC 
AACAACCCGG GCAACCCGTC CAACCCCGGC CCCCCCAAGA CTCCCGAGAA CAGCCTTATC 
GTCACCAACC CCAGCAGCAC CCAGCCCAAC TCAGCAAAGA CTGCCAGGAA ACCCGAGCAC 
ATGGCGGTGG AGATCCCCCC GGCCTGCCCG CCCCTCAACC ACACTGTGGT CCAAGTAAAC 
AAAAACGCCA ACCCAGACCC ACTGCCAAAG AAAGAGGAAG AGAAGAAGGA GGAAGAGGAG 
GCAGACCCCG GGGAGGATGG CCCAAAGCCC ATGCCGCCCT ACAGCTCCAT GTTCATCCTC 
TCCACCACCA ACCCCCTTCG CCGGCTGTGC CATTACATCC TGAACCTGCG CTACTTCGAG 
ATGTGCATCC TCATGGTCAT TGCCATGAGT AGCATCGCGC TGGCCGCCGA GGACCCGGTG 
CAGCCCAACG CACCCCGCAA CAACGTGCTG CGATATTTTG ACTATGTTTT CACAGGAGTG 
TTTACCTTTG AGATGGTGAT CAAGATGATC GACCTGGGCC TCGTCCTGCA TCAGGGGGCC 
TATTTCCGTG ACCTGTGGAA CATTCTGGAC TTCATAGTGG TCAGTGGGGC CCTGGTGGCC 
TTTGCCTTCA CGAGCAGTAC ACGTGGCAAT AGCAAAGGAA AGGACATCAA CACCATCAAG 
TCCCTCCGAG TCCTCCGGGT GCTACGACCT CTAAAGACCA TCAAGCGGCT GCCTAAGTTG 
AAGGCCGTAT TTGACTGCGT GGTGAACTCG CTCAAGAACG TCTTCAACAT CCTCATTGTC 
TACATGCTCT TCATGTTCAT CTTCGCCGTG GTGGCCGTGC AGCTCTTCAA GGGCAAATTC 
TTCCACTGCA CGGACGAGTC CAAGGAGTTT GAGAGAGACT GTCGAGGCAA ATACCTCCTT 
TACGAGAAGA ACGAGGTAAA GGCGCGGGAC CGCGAGTGGA AGAAATACGA CTTCCACTAC 
GACAACGTGC TCTGGGCCCT GCTCACGCTC TTTACGGTGT CCACGGGAGA GGGCTGGCCA 
CAGGTCCTCA AGCACTCAGT GGATGCCACT TTTGAGAACC AGGGCCCCAG CCCCGGGTAC 
CGCATGGAAA TGTCCATCTT CTACGTGGTC TACTTTGTGG TGTTTCCCTT CTTCTTTGTC 
AATATCTTTG TGGCCTTGAT CATCATCACC TTCCAGGAGC AGGGAGACAA GATGATGGAA 
GAATACAGCC TAGAGAAAAA TGAGAGGGCC TGCATCGACT TTGCCATCAG TGCCAAGCCG 
CTGACCAGGC ACATGCCCCA GAACAAGCAG AGCTTCCAGT ATCGAATGTG GCAGTTCGTG 
GTGTCCCCAC CCTTTGAGTA CACCATCATG GCCATGATCG CTCTCAACAC CATCGTGCTA 
ATGATGAAGT TCTATGGAGC CTCTGTGGCC TATGAAAACG CCCTTCGAGT GTTCAACATT 
GTCTTCACCT CCCTCTTCTC TCTCGAATGT GTGCTCAAAG TCATGGCTTT TGGGATTCTG 
AATTATTTCC GCGATGCCTG GAACATCTTC GACTTTGTGA CTGTTCTGGG CAGCATCACA 
GACATCCTCG TCACCGAGTT TGGGAATCCG AATAACTTCA TCAACCTGAG CTTTCTCCGC 
CTCTTCCGTG CTGCCCGACT CATCAAACTC CTCCGCCAGG GTTACACCAT CCGCATTCTC 
CTCTGGACTT TCGTGCAGTC TTTCAAGGCC CTACCTTATG TCTGTCTGCT GATCGCCATG 
CTCTTCTTCA TCTATGCCAT CATCGGGATG CAGGTGTTTG GCAACATCGG CATTGATGGG 
GAAGATGAGG ACAGCGATGA GGATGAGTTC CAAATCACGG AGCACAATAA CTTCCGGACC 
TTCTTCCAAG CTCTCATGCT TCTCTTCCGG AGCGCCACAG GGGAAGCGTG GCACAACATC 
ATGCTGTCCT GCCTCAGCGG GAAGCCATGC GACAAGAACT CCGGGATCCA AAAACCAGAG 
TGTGGCAACG AGTTCGCCTA TTTTTACTTT GTCTCGTTCA TCTTCCTTTG CTCATTTCTG 
ATGCTGAATC TCTTTGTTGC TGTCATCATG GACAACTTCG AGTACCTCAC CCGAGATTCC 
TCCATCCTGG GCCCCCACCA CCTGGATGAG TACGTGCGTG TCTGGGCAGA GTATGACCCT 
GCTGCCTGCG GCCGCATTCA CTATAAGGAC ATGTACAGTT TATTGCGAGT AATATCGCCC 
CCTCTCGGCT TAGGCAAGAA ATGTCCTCAT AGGGTTGCTT GCAAGAGGCT CTTGCGGATG 
GACCTACCCG TAGCGGATGA CAACACCGTT CACTTCAACT CCACCTTGAT GGCTCTGATC 
CGAACCGCCC TGGATATCAA AATCGCAAAG GGTGGAGCTG ACAAGCAGCA AATGGACGCA 
GAGCTCCGCA AGGAAATGAT GGCCATTTGG CCCAACCTGT CTCAGAAGAC CTTGGATCTG 
CTGGTCACAC CTCACAAGTC CACGGACCTG ACAGTGGGTA AGATCTACGC AGCCATGATG 
ATCATGGAGT ACTACCGGCA GAGCAAGGCC AAGAAGCTGC AGGCCATGCG AGAGGAGCAG 
AACCGGACAC CACTCATGTT CCAGCGCATG GAGCCTCCAT CGCCAACACA GGAGGGAGGA 
CCCAGCCAAA ACGCCCTTCC CTCCACTCAG CTGGACCCCG GAGGAGGCCT GATGGCTCAA 
GAAAGCAGCA TGAAGGAGAG CCCGTCCTGG GTGACCCAGC GGGCACAGGA GATGTTCCAG 
AAGACTGGTA CCTGGAGCCC AGAGCGAGGG CCACCCATCG ACATGCCTAA CAGCCAGCCC 
AACTCCCAGT CTGTGGAGAT GAGAGAAATG GGAACTGATG GCTACTCAGA CAGCGAACAC 
TACCTCCCCA TGGAAGGACA GACCAGGGCC GCCTCCATGC CCCGCCTCCC AGCAGAGAAC 
CAGAGGAGAA GGGGCCGGCC ACGTGGAAAT AACCTCAGTA CCATCTCTGA TACCAGCCCC 
ATGAAGCGCT CAGCCTCCGT GCTGGGACCC AAAGCCCGGC GACTGGATGA CTACTCACTA 
GAGCGGGTAC CACCTGAGGA GAACCAAAGG TACCACCAAC GCCGCCGGGA CCGTGGCCAC 
CGCACCTCTG AGCGCTCTCT GGGCCGATAC ACTGATGTGG ACACAGGCCT GGGGACAGAT 
CTGAGCATGA CCACCCAATC GGGTGACCTG CCCTCCAAAG ATCGGGACCA GGACCGGGGC 
CGGCCCAAGG ACCGGAAGCA TCGGCCACAC CACCACCACC ACCATCATCA CCATCATCCC 
CCGGCCCCGG ACCGGGAGCG CTACGCACAG GAGCGGCCGG ACACCGGCCG GGCGCGGGCC 
CGGGAGCAGC GCTGGTCCCG CTCGCCCAGC GAGGGTCGGG AGCACGCGAC ACACAGACAG 
GGCAGTAGTT CCGTCAGTGG AAGCCCAGCC CCCTCTACGT CCGGCACCAG CACGCCGCGG 
CGCGGCCGCC GCCAGCTCCC CCAGACCCCC TGCACCCCGC GGCCGCTTGT GTCCTACTCG 
CCCGCTCCGC GCAGGCCTGC GGCGCGCAGG ATGGCGGGTC CCCCAGCGCC CCCTGGCGGC 
TCGCCGCGGG GTTGCCGCCG TGCGCCGCGC TGGCCTGCGC ATGCGCCTGA GGGCCCGCGG 
CCGCGCGGTG CCGACTACAC CGAGCCCGAC AGCCCGCGCG AGCCGCCAGG GGGCGCGCAT 
GAGCCCGCGC CGCGGTCGCC CAGGACTCCG CGCGCCGCGG GCTGCGCGTC ACCGCGCCAC 
GGCCGCAGGC TGCCCAATGG TTACTACGCG GGGCACGGCG CGCCACGGCC GCGCACAGCC 
CGCAGGGGCG CGCACGACGC GTACAGCGAG AGCGAGGACG ACTGGTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017456667.1
CDS	105..7535
Translation	MVSSPGISERGPWMPRASWVSDPMRSPLNHELLHSSRYPGTDCTLAGSSRTLHVPWSEQMSGSLEGPTLKRRGGVVDHRDVILAHQAHKMHSTPQARRKEWEMARFGDEMPGRYGAGGGGSGPAAGVVVGAAGGRGAGGSRQGGQPGAQRMYKQSMAQRARTMALYNPIPVRQNCLTVNRSLFLFSEDNVVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDDDKTPMSERLDDTEPYFIGIFCFEAGIKIVALGFAFHKGSYLRNGWNVMDFVVVLTGILATVGTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILIFAIIGLEFYMGKFHTTCFEEGTDDIQGESPAPCGTEEPARTCPNGTKCQPYWEGPNNGITQFDNILFAVLTVFQCITMEGWTDLLYNSNDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYMEWISKAEEVILAEDETDVEQRHPFDGALRRATLKKSKTDLLNPEEAEDQLADIASVGSPFARASIKSAKLENSTFFHKKERRMRFYIRRMVKTQAFYWTVLSLVALNTLCVAIVHYNQPEWLSDFLYYAEFIFLGLFMSEMFIKMYGLGTRPYFHSSFNCFDCGVIIGSIFEVIWAVIKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFDEGTPPTNFDTFPAAIMTVFQILTGEDWNEVMYDGIKSQGGVQGGMVFSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAANQKLALQKAKEVAEVSPLSAANMSIAVKEQQKNQKPAKSVWEQRTSEMRKQNLLASREALYGDAAERWPTTYARPLRPDVKTHLDRPLVVDPQENRNNNTNKSRAPEALRQTARPRESARDPDARRAWPSSPERAPGREGPYGRESEPQQREHAPPREHVPWDADPERAKAGDAPRRHTHRPVAEGEPRRHRARRRPGDEPDDRPERRPRPRDATRPARAADGEGDDGERKRRHRHGPPAHDDRERRHRRRKESQGSGVPMSGPNLSTTRPIQQDLGRQDLPLAEDLDNMKNNKLATGEPASPHDSLGHSGLPPSPAKIGNSTNPGPALATNPQNAASRRTPNNPGNPSNPGPPKTPENSLIVTNPSSTQPNSAKTARKPEHMAVEIPPACPPLNHTVVQVNKNANPDPLPKKEEEKKEEEEADPGEDGPKPMPPYSSMFILSTTNPLRRLCHYILNLRYFEMCILMVIAMSSIALAAEDPVQPNAPRNNVLRYFDYVFTGVFTFEMVIKMIDLGLVLHQGAYFRDLWNILDFIVVSGALVAFAFTSSTRGNSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYMLFMFIFAVVAVQLFKGKFFHCTDESKEFERDCRGKYLLYEKNEVKARDREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATFENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEEYSLEKNERACIDFAISAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYTIMAMIALNTIVLMMKFYGASVAYENALRVFNIVFTSLFSLECVLKVMAFGILNYFRDAWNIFDFVTVLGSITDILVTEFGNPNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIGIDGEDEDSDEDEFQITEHNNFRTFFQALMLLFRSATGEAWHNIMLSCLSGKPCDKNSGIQKPECGNEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRVWAEYDPAACGRIHYKDMYSLLRVISPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKQQMDAELRKEMMAIWPNLSQKTLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKKLQAMREEQNRTPLMFQRMEPPSPTQEGGPSQNALPSTQLDPGGGLMAQESSMKESPSWVTQRAQEMFQKTGTWSPERGPPIDMPNSQPNSQSVEMREMGTDGYSDSEHYLPMEGQTRAASMPRLPAENQRRRGRPRGNNLSTISDTSPMKRSASVLGPKARRLDDYSLERVPPEENQRYHQRRRDRGHRTSERSLGRYTDVDTGLGTDLSMTTQSGDLPSKDRDQDRGRPKDRKHRPHHHHHHHHHHPPAPDRERYAQERPDTGRARAREQRWSRSPSEGREHATHRQGSSSVSGSPAPSTSGTSTPRRGRRQLPQTPCTPRPLVSYSPAPRRPAARRMAGPPAPPGGSPRGCRRAPRWPAHAPEGPRPRGADYTEPDSPREPPGGAHEPAPRSPRTPRAAGCASPRHGRRLPNGYYAGHGAPRPRTARRGAHDAYSESEDDWC

Target ORF information:

RefSeq Version	XM_017601178.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel subunit alpha1 A (Cacna1a), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017601178.1

ORF Insert Sequence:

ATGGTTTCCT CTCCTGGGAT TTCTGAGCGA GGCCCATGGA TGCCGAGGGC CTCCTGGGTC 
TCTGACCCCA TGCGTTCTCC CCTCAATCAT GAGCTCCTGC ACAGCTCGAG GTACCCAGGC 
ACGGACTGCA CCCTGGCAGG TTCTTCCAGG ACTTTGCATG TCCCGTGGTC CGAGCAGATG 
AGCGGAAGCC TCGAGGGGCC AACTCTGAAA CGGCGTGGTG GCGTGGTGGA CCACCGGGAT 
GTCATCTTGG CCCACCAGGC GCACAAAATG CACAGCACCC CTCAGGCCAG GAGGAAAGAG 
TGGGAAATGG CCCGCTTTGG AGACGAGATG CCGGGCCGCT ACGGCGCAGG CGGAGGAGGC 
TCAGGGCCGG CCGCCGGGGT GGTCGTGGGC GCCGCGGGCG GCCGAGGAGC CGGGGGCAGC 
CGGCAGGGCG GGCAGCCCGG AGCGCAGAGG ATGTACAAGC AGTCGATGGC GCAGAGAGCG 
CGGACCATGG CCCTCTACAA CCCCATCCCT GTCCGCCAGA ACTGCCTCAC GGTCAACCGC 
TCCCTGTTCC TCTTCAGTGA AGACAACGTG GTGAGAAAAT ACGCCAAAAA GATCACGGAA 
TGGCCTCCCT TCGAGTACAT GATCCTGGCC ACCATCATTG CTAACTGCAT CGTCCTGGCC 
CTGGAGCAGC ACCTCCCTGA TGATGACAAG ACACCCATGT CCGAGCGGCT GGATGACACA 
GAACCCTATT TCATTGGCAT CTTCTGTTTT GAGGCTGGAA TTAAGATCGT GGCTCTTGGC 
TTTGCCTTCC ACAAAGGCTC CTACCTGAGG AATGGCTGGA ACGTCATGGA CTTTGTCGTG 
GTGCTAACAG GCATCTTGGC CACTGTCGGG ACGGAGTTTG ATCTACGGAC ACTGAGGGCG 
GTTCGTGTGC TGCGGCCACT CAAGCTGGTG TCTGGAATCC CAAGTTTACA AGTCGTCCTG 
AAGTCAATCA TGAAGGCGAT GATCCCTCTG CTGCAGATCG GCCTCCTCCT GTTTTTTGCA 
ATCCTTATTT TTGCAATCAT AGGGTTAGAA TTTTATATGG GAAAATTTCA TACCACCTGC 
TTTGAAGAGG GGACAGACGA CATCCAGGGT GAGTCGCCAG CTCCGTGTGG GACAGAGGAG 
CCTGCCCGCA CCTGCCCCAA CGGGACCAAA TGTCAGCCGT ACTGGGAAGG GCCCAACAAC 
GGCATCACTC AGTTCGACAA CATCCTGTTT GCTGTGCTCA CTGTTTTCCA GTGCATCACC 
ATGGAAGGCT GGACTGATCT CCTCTACAAT AGCAACGATG CCTCAGGGAA CACTTGGAAC 
TGGTTGTACT TCATCCCCCT CATCATCATC GGCTCCTTTT TTATGCTGAA CCTTGTGCTG 
GGTGTGCTGT CTGGGGAGTT TGCCAAAGAA AGGGAACGTG TAGAGAACCG AAGGGCTTTT 
CTGAAGCTCA GAAGACAACA GCAGATTGAA CGTGAGCTCA ATGGATACAT GGAGTGGATC 
TCGAAAGCAG AAGAGGTGAT CCTCGCGGAG GACGAGACAG ACGTGGAGCA GAGGCACCCT 
TTTGATGGAG CTCTTCGGAG AGCTACTCTG AAGAAAAGCA AGACGGACCT GCTCAACCCT 
GAGGAGGCGG AGGACCAGCT TGCTGACATC GCGTCTGTGG GGTCTCCCTT CGCCAGAGCC 
AGCATCAAAA GTGCCAAGCT GGAGAATTCG ACTTTTTTCC ACAAAAAGGA GAGAAGAATG 
CGTTTCTACA TCCGCCGCAT GGTCAAAACT CAGGCCTTCT ACTGGACCGT GCTCAGTCTG 
GTAGCCCTCA ACACGCTGTG CGTCGCCATT GTCCACTACA ACCAGCCCGA GTGGCTCTCC 
GACTTCCTCT ACTATGCAGA ATTCATTTTC TTAGGACTCT TTATGTCCGA AATGTTTATA 
AAAATGTATG GGCTCGGGAC ACGGCCTTAC TTCCACTCTT CCTTCAACTG CTTTGACTGT 
GGGGTCATCA TCGGGAGCAT CTTTGAAGTC ATCTGGGCCG TCATCAAACC GGGTACATCC 
TTTGGAATCA GCGTGTTACG AGCTCTCAGG TTACTGCGTA TTTTCAAAGT CACAAAGTAC 
TGGGCATCTC TCAGAAACCT GGTTGTCTCC CTCCTCAACT CCATGAAATC CATCATAAGT 
CTGCTGTTCC TCCTCTTCCT CTTCATTGTC GTCTTTGCCC TCTTGGGGAT GCAGCTGTTT 
GGTGGCCAGT TTAATTTTGA CGAGGGGACT CCTCCCACCA ACTTCGACAC TTTTCCAGCA 
GCAATAATGA CTGTGTTTCA GATCCTGACT GGCGAGGATT GGAATGAGGT CATGTATGAT 
GGGATCAAGT CTCAGGGGGG CGTGCAGGGC GGCATGGTGT TCTCCATCTA CTTCATCGTC 
CTCACCCTCT TCGGGAACTA CACCCTGCTG AACGTGTTCT TGGCTATCGC GGTGGACAAC 
CTGGCCAACG CCCAGGAACT CACCAAGGAT GAACAAGAAG AGGAAGAGGC AGCCAATCAG 
AAACTGGCTC TACAGAAAGC CAAGGAGGTG GCAGAAGTGA GTCCCCTGTC TGCAGCCAAC 
ATGTCCATAG CTGTGAAGGA ACAGCAGAAG AACCAGAAGC CTGCCAAGTC GGTGTGGGAG 
CAGCGCACCA GCGAGATGCG CAAGCAGAAC CTGCTGGCTA GCCGCGAGGC GCTGTACGGG 
GACGCGGCTG AGCGCTGGCC CACCACTTAC GCGCGCCCGC TGCGGCCGGA CGTGAAGACG 
CACTTGGACC GGCCGCTCGT GGTGGACCCG CAGGAGAACC GTAACAACAA CACCAACAAG 
AGCCGTGCGC CAGAAGCGCT GCGCCAAACC GCGCGGCCCC GCGAGAGCGC GCGCGACCCC 
GACGCGCGGC GCGCCTGGCC CAGCAGCCCT GAGCGCGCCC CTGGACGAGA GGGCCCGTAT 
GGCCGCGAGA GCGAGCCGCA ACAGCGCGAG CACGCGCCAC CCCGCGAGCA CGTACCCTGG 
GACGCGGATC CTGAGCGCGC CAAGGCCGGG GACGCGCCCC GCCGCCACAC GCACCGGCCT 
GTGGCCGAGG GCGAGCCTCG TCGCCACCGC GCGCGCCGCC GGCCCGGGGA CGAACCGGAC 
GACAGACCGG AGCGCAGGCC GCGTCCCCGC GACGCCACTA GGCCGGCCCG CGCTGCAGAC 
GGCGAAGGCG ATGATGGGGA GCGCAAGCGG CGACACCGAC ACGGGCCGCC GGCCCACGAT 
GACAGGGAGC GCAGACACCG GCGCAGAAAA GAGAGCCAGG GCTCTGGGGT CCCCATGTCT 
GGTCCCAACC TGTCCACCAC CAGGCCAATC CAGCAGGATC TGGGCCGCCA GGACCTGCCA 
CTGGCTGAGG ACCTGGACAA CATGAAGAAC AACAAGTTGG CCACCGGGGA GCCTGCCAGT 
CCCCACGACA GCCTGGGCCA CAGTGGCCTT CCCCCTAGCC CTGCCAAGAT CGGGAACAGC 
ACCAACCCTG GTCCCGCCTT GGCCACCAAT CCCCAGAATG CTGCCAGCCG CAGGACGCCC 
AACAACCCGG GCAACCCGTC CAACCCCGGC CCCCCCAAGA CTCCCGAGAA CAGCCTTATC 
GTCACCAACC CCAGCAGCAC CCAGCCCAAC TCAGCAAAGA CTGCCAGGAA ACCCGAGCAC 
ATGGCGGTGG AGATCCCCCC GGCCTGCCCG CCCCTCAACC ACACTGTGGT CCAAGTAAAC 
AAAAACGCCA ACCCAGACCC ACTGCCAAAG AAAGAGGAAG AGAAGAAGGA GGAAGAGGAG 
GCAGACCCCG GGGAGGATGG CCCAAAGCCC ATGCCGCCCT ACAGCTCCAT GTTCATCCTC 
TCCACCACCA ACCCCCTTCG CCGGCTGTGC CATTACATCC TGAACCTGCG CTACTTCGAG 
ATGTGCATCC TCATGGTCAT TGCCATGAGT AGCATCGCGC TGGCCGCCGA GGACCCGGTG 
CAGCCCAACG CACCCCGCAA CAACGTGCTG CGATATTTTG ACTATGTTTT CACAGGAGTG 
TTTACCTTTG AGATGGTGAT CAAGATGATC GACCTGGGCC TCGTCCTGCA TCAGGGGGCC 
TATTTCCGTG ACCTGTGGAA CATTCTGGAC TTCATAGTGG TCAGTGGGGC CCTGGTGGCC 
TTTGCCTTCA CGAGCAGTAC ACGTGGCAAT AGCAAAGGAA AGGACATCAA CACCATCAAG 
TCCCTCCGAG TCCTCCGGGT GCTACGACCT CTAAAGACCA TCAAGCGGCT GCCTAAGTTG 
AAGGCCGTAT TTGACTGCGT GGTGAACTCG CTCAAGAACG TCTTCAACAT CCTCATTGTC 
TACATGCTCT TCATGTTCAT CTTCGCCGTG GTGGCCGTGC AGCTCTTCAA GGGCAAATTC 
TTCCACTGCA CGGACGAGTC CAAGGAGTTT GAGAGAGACT GTCGAGGCAA ATACCTCCTT 
TACGAGAAGA ACGAGGTAAA GGCGCGGGAC CGCGAGTGGA AGAAATACGA CTTCCACTAC 
GACAACGTGC TCTGGGCCCT GCTCACGCTC TTTACGGTGT CCACGGGAGA GGGCTGGCCA 
CAGGTCCTCA AGCACTCAGT GGATGCCACT TTTGAGAACC AGGGCCCCAG CCCCGGGTAC 
CGCATGGAAA TGTCCATCTT CTACGTGGTC TACTTTGTGG TGTTTCCCTT CTTCTTTGTC 
AATATCTTTG TGGCCTTGAT CATCATCACC TTCCAGGAGC AGGGAGACAA GATGATGGAA 
GAATACAGCC TAGAGAAAAA TGAGAGGGCC TGCATCGACT TTGCCATCAG TGCCAAGCCG 
CTGACCAGGC ACATGCCCCA GAACAAGCAG AGCTTCCAGT ATCGAATGTG GCAGTTCGTG 
GTGTCCCCAC CCTTTGAGTA CACCATCATG GCCATGATCG CTCTCAACAC CATCGTGCTA 
ATGATGAAGT TCTATGGAGC CTCTGTGGCC TATGAAAACG CCCTTCGAGT GTTCAACATT 
GTCTTCACCT CCCTCTTCTC TCTCGAATGT GTGCTCAAAG TCATGGCTTT TGGGATTCTG 
AATTATTTCC GCGATGCCTG GAACATCTTC GACTTTGTGA CTGTTCTGGG CAGCATCACA 
GACATCCTCG TCACCGAGTT TGGGAATCCG AATAACTTCA TCAACCTGAG CTTTCTCCGC 
CTCTTCCGTG CTGCCCGACT CATCAAACTC CTCCGCCAGG GTTACACCAT CCGCATTCTC 
CTCTGGACTT TCGTGCAGTC TTTCAAGGCC CTACCTTATG TCTGTCTGCT GATCGCCATG 
CTCTTCTTCA TCTATGCCAT CATCGGGATG CAGGTGTTTG GCAACATCGG CATTGATGGG 
GAAGATGAGG ACAGCGATGA GGATGAGTTC CAAATCACGG AGCACAATAA CTTCCGGACC 
TTCTTCCAAG CTCTCATGCT TCTCTTCCGG AGCGCCACAG GGGAAGCGTG GCACAACATC 
ATGCTGTCCT GCCTCAGCGG GAAGCCATGC GACAAGAACT CCGGGATCCA AAAACCAGAG 
TGTGGCAACG AGTTCGCCTA TTTTTACTTT GTCTCGTTCA TCTTCCTTTG CTCATTTCTG 
ATGCTGAATC TCTTTGTTGC TGTCATCATG GACAACTTCG AGTACCTCAC CCGAGATTCC 
TCCATCCTGG GCCCCCACCA CCTGGATGAG TACGTGCGTG TCTGGGCAGA GTATGACCCT 
GCTGCCTGCG GCCGCATTCA CTATAAGGAC ATGTACAGTT TATTGCGAGT AATATCGCCC 
CCTCTCGGCT TAGGCAAGAA ATGTCCTCAT AGGGTTGCTT GCAAGAGGCT CTTGCGGATG 
GACCTACCCG TAGCGGATGA CAACACCGTT CACTTCAACT CCACCTTGAT GGCTCTGATC 
CGAACCGCCC TGGATATCAA AATCGCAAAG GGTGGAGCTG ACAAGCAGCA AATGGACGCA 
GAGCTCCGCA AGGAAATGAT GGCCATTTGG CCCAACCTGT CTCAGAAGAC CTTGGATCTG 
CTGGTCACAC CTCACAAGTC CACGGACCTG ACAGTGGGTA AGATCTACGC AGCCATGATG 
ATCATGGAGT ACTACCGGCA GAGCAAGGCC AAGAAGCTGC AGGCCATGCG AGAGGAGCAG 
AACCGGACAC CACTCATGTT CCAGCGCATG GAGCCTCCAT CGCCAACACA GGAGGGAGGA 
CCCAGCCAAA ACGCCCTTCC CTCCACTCAG CTGGACCCCG GAGGAGGCCT GATGGCTCAA 
GAAAGCAGCA TGAAGGAGAG CCCGTCCTGG GTGACCCAGC GGGCACAGGA GATGTTCCAG 
AAGACTGGTA CCTGGAGCCC AGAGCGAGGG CCACCCATCG ACATGCCTAA CAGCCAGCCC 
AACTCCCAGT CTGTGGAGAT GAGAGAAATG GGAACTGATG GCTACTCAGA CAGCGAACAC 
TACCTCCCCA TGGAAGGACA GACCAGGGCC GCCTCCATGC CCCGCCTCCC AGCAGAGAAC 
CAGAGGAGAA GGGGCCGGCC ACGTGGAAAT AACCTCAGTA CCATCTCTGA TACCAGCCCC 
ATGAAGCGCT CAGCCTCCGT GCTGGGACCC AAAGCCCGGC GACTGGATGA CTACTCACTA 
GAGCGGGTAC CACCTGAGGA GAACCAAAGG TACCACCAAC GCCGCCGGGA CCGTGGCCAC 
CGCACCTCTG AGCGCTCTCT GGGCCGATAC ACTGATGTGG ACACAGGCCT GGGGACAGAT 
CTGAGCATGA CCACCCAATC GGGTGACCTG CCCTCCAAAG ATCGGGACCA GGACCGGGGC 
CGGCCCAAGG ACCGGAAGCA TCGGCCACAC CACCACCACC ACCATCATCA CCATCATCCC 
CCGGCCCCGG ACCGGGAGCG CTACGCACAG GAGCGGCCGG ACACCGGCCG GGCGCGGGCC 
CGGGAGCAGC GCTGGTCCCG CTCGCCCAGC GAGGGTCGGG AGCACGCGAC ACACAGACAG 
GGCAGTAGTT CCGTCAGTGG AAGCCCAGCC CCCTCTACGT CCGGCACCAG CACGCCGCGG 
CGCGGCCGCC GCCAGCTCCC CCAGACCCCC TGCACCCCGC GGCCGCTTGT GTCCTACTCG 
CCCGCTCCGC GCAGGCCTGC GGCGCGCAGG ATGGCGGGTC CCCCAGCGCC CCCTGGCGGC 
TCGCCGCGGG GTTGCCGCCG TGCGCCGCGC TGGCCTGCGC ATGCGCCTGA GGGCCCGCGG 
CCGCGCGGTG CCGACTACAC CGAGCCCGAC AGCCCGCGCG AGCCGCCAGG GGGCGCGCAT 
GAGCCCGCGC CGCGGTCGCC CAGGACTCCG CGCGCCGCGG GCTGCGCGTC ACCGCGCCAC 
GGCCGCAGGC TGCCCAATGG TTACTACGCG GGGCACGGCG CGCCACGGCC GCGCACAGCC 
CGCAGGGGCG CGCACGACGC GTACAGCGAG AGCGAGGACG ACTGGTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1a ( XM_017601179.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017456668.1 Rattus norvegicus calcium voltage-gated channel subunit alpha1 A (Cacna1a), transcript variant X10, mRNA.

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CloneID	ORa59373
Clone ID Related Accession (Same CDS sequence)	XM_017601179.1
Accession Version	XM_017601179.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7425bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTCCT CTCCTGGGAT TTCTGAGCGA GGCCCATGGA TGCCGAGGGC CTCCTGGGTC 
TCTGACCCCA TGCGTTCTCC CCTCAATCAT GAGCTCCTGC ACAGCTCGAG GTACCCAGGC 
ACGGACTGCA CCCTGGCAGG TTCTTCCAGG ACTTTGCATG TCCCGTGGTC CGAGCAGATG 
AGCGGAAGCC TCGAGGGGCC AACTCTGAAA CGGCGTGGTG GCGTGGTGGA CCACCGGGAT 
GTCATCTTGG CCCACCAGGC GCACAAAATG CACAGCACCC CTCAGGCCAG GAGGAAAGAG 
TGGGAAATGG CCCGCTTTGG AGACGAGATG CCGGGCCGCT ACGGCGCAGG CGGAGGAGGC 
TCAGGGCCGG CCGCCGGGGT GGTCGTGGGC GCCGCGGGCG GCCGAGGAGC CGGGGGCAGC 
CGGCAGGGCG GGCAGCCCGG AGCGCAGAGG ATGTACAAGC AGTCGATGGC GCAGAGAGCG 
CGGACCATGG CCCTCTACAA CCCCATCCCT GTCCGCCAGA ACTGCCTCAC GGTCAACCGC 
TCCCTGTTCC TCTTCAGTGA AGACAACGTG GTGAGAAAAT ACGCCAAAAA GATCACGGAA 
TGGCCTCCCT TCGAGTACAT GATCCTGGCC ACCATCATTG CTAACTGCAT CGTCCTGGCC 
CTGGAGCAGC ACCTCCCTGA TGATGACAAG ACACCCATGT CCGAGCGGCT GGATGACACA 
GAACCCTATT TCATTGGCAT CTTCTGTTTT GAGGCTGGAA TTAAGATCGT GGCTCTTGGC 
TTTGCCTTCC ACAAAGGCTC CTACCTGAGG AATGGCTGGA ACGTCATGGA CTTTGTCGTG 
GTGCTAACAG GCATCTTGGC CACTGTCGGG ACGGAGTTTG ATCTACGGAC ACTGAGGGCG 
GTTCGTGTGC TGCGGCCACT CAAGCTGGTG TCTGGAATCC CAAGTTTACA AGTCGTCCTG 
AAGTCAATCA TGAAGGCGAT GATCCCTCTG CTGCAGATCG GCCTCCTCCT GTTTTTTGCA 
ATCCTTATTT TTGCAATCAT AGGGTTAGAA TTTTATATGG GAAAATTTCA TACCACCTGC 
TTTGAAGAGG GGACAGACGA CATCCAGGGT GAGTCGCCAG CTCCGTGTGG GACAGAGGAG 
CCTGCCCGCA CCTGCCCCAA CGGGACCAAA TGTCAGCCGT ACTGGGAAGG GCCCAACAAC 
GGCATCACTC AGTTCGACAA CATCCTGTTT GCTGTGCTCA CTGTTTTCCA GTGCATCACC 
ATGGAAGGCT GGACTGATCT CCTCTACAAT AGCAACGATG CCTCAGGGAA CACTTGGAAC 
TGGTTGTACT TCATCCCCCT CATCATCATC GGCTCCTTTT TTATGCTGAA CCTTGTGCTG 
GGTGTGCTGT CTGGGGAGTT TGCCAAAGAA AGGGAACGTG TAGAGAACCG AAGGGCTTTT 
CTGAAGCTCA GAAGACAACA GCAGATTGAA CGTGAGCTCA ATGGATACAT GGAGTGGATC 
TCGAAAGCAG AAGAGGTGAT CCTCGCGGAG GACGAGACAG ACGTGGAGCA GAGGCACCCT 
TTTGATGGAG CTCTTCGGAG AGCTACTCTG AAGAAAAGCA AGACGGACCT GCTCAACCCT 
GAGGAGGCGG AGGACCAGCT TGCTGACATC GCGTCTGTGG GGTCTCCCTT CGCCAGAGCC 
AGCATCAAAA GTGCCAAGCT GGAGAATTCG ACTTTTTTCC ACAAAAAGGA GAGAAGAATG 
CGTTTCTACA TCCGCCGCAT GGTCAAAACT CAGGCCTTCT ACTGGACCGT GCTCAGTCTG 
GTAGCCCTCA ACACGCTGTG CGTCGCCATT GTCCACTACA ACCAGCCCGA GTGGCTCTCC 
GACTTCCTCT ACTATGCAGA ATTCATTTTC TTAGGACTCT TTATGTCCGA AATGTTTATA 
AAAATGTATG GGCTCGGGAC ACGGCCTTAC TTCCACTCTT CCTTCAACTG CTTTGACTGT 
GGGGTCATCA TCGGGAGCAT CTTTGAAGTC ATCTGGGCCG TCATCAAACC GGGTACATCC 
TTTGGAATCA GCGTGTTACG AGCTCTCAGG TTACTGCGTA TTTTCAAAGT CACAAAGTAC 
TGGGCATCTC TCAGAAACCT GGTTGTCTCC CTCCTCAACT CCATGAAATC CATCATAAGT 
CTGCTGTTCC TCCTCTTCCT CTTCATTGTC GTCTTTGCCC TCTTGGGGAT GCAGCTGTTT 
GGTGGCCAGT TTAATTTTGA CGAGGGGACT CCTCCCACCA ACTTCGACAC TTTTCCAGCA 
GCAATAATGA CTGTGTTTCA GATCCTGACT GGCGAGGATT GGAATGAGGT CATGTATGAT 
GGGATCAAGT CTCAGGGGGG CGTGCAGGGC GGCATGGTGT TCTCCATCTA CTTCATCGTC 
CTCACCCTCT TCGGGAACTA CACCCTGCTG AACGTGTTCT TGGCTATCGC GGTGGACAAC 
CTGGCCAACG CCCAGGAACT CACCAAGGAT GAACAAGAAG AGGAAGAGGC AGCCAATCAG 
AAACTGGCTC TACAGAAAGC CAAGGAGGTG GCAGAAGTGA GTCCCCTGTC TGCAGCCAAC 
ATGTCCATAG CTGTGAAGGA ACAGCAGAAG AACCAGAAGC CTGCCAAGTC GGTGTGGGAG 
CAGCGCACCA GCGAGATGCG CAAGCAGAAC CTGCTGGCTA GCCGCGAGGC GCTGTACGGG 
GACGCGGCTG AGCGCTGGCC CACCACTTAC GCGCGCCCGC TGCGGCCGGA CGTGAAGACG 
CACTTGGACC GGCCGCTCGT GGTGGACCCG CAGGAGAACC GTAACAACAA CACCAACAAG 
AGCCGTGCGC CAGAAGCGCT GCGCCAAACC GCGCGGCCCC GCGAGAGCGC GCGCGACCCC 
GACGCGCGGC GCGCCTGGCC CAGCAGCCCT GAGCGCGCCC CTGGACGAGA GGGCCCGTAT 
GGCCGCGAGA GCGAGCCGCA ACAGCGCGAG CACGCGCCAC CCCGCGAGCA CGTACCCTGG 
GACGCGGATC CTGAGCGCGC CAAGGCCGGG GACGCGCCCC GCCGCCACAC GCACCGGCCT 
GTGGCCGAGG GCGAGCCTCG TCGCCACCGC GCGCGCCGCC GGCCCGGGGA CGAACCGGAC 
GACAGACCGG AGCGCAGGCC GCGTCCCCGC GACGCCACTA GGCCGGCCCG CGCTGCAGAC 
GGCGAAGGCG ATGATGGGGA GCGCAAGCGG CGACACCGAC ACGGGCCGCC GGCCCACGAT 
GACAGGGAGC GCAGACACCG GCGCAGAAAA GAGAGCCAGG GCTCTGGGGT CCCCATGTCT 
GGTCCCAACC TGTCCACCAC CAGGCCAATC CAGCAGGATC TGGGCCGCCA GGACCTGCCA 
CTGGCTGAGG ACCTGGACAA CATGAAGAAC AACAAGTTGG CCACCGGGGA GCCTGCCAGT 
CCCCACGACA GCCTGGGCCA CAGTGGCCTT CCCCCTAGCC CTGCCAAGAT CGGGAACAGC 
ACCAACCCTG GTCCCGCCTT GGCCACCAAT CCCCAGAATG CTGCCAGCCG CAGGACGCCC 
AACAACCCGG GCAACCCGTC CAACCCCGGC CCCCCCAAGA CTCCCGAGAA CAGCCTTATC 
GTCACCAACC CCAGCAGCAC CCAGCCCAAC TCAGCAAAGA CTGCCAGGAA ACCCGAGCAC 
ATGGCGGTGG AGATCCCCCC GGCCTGCCCG CCCCTCAACC ACACTGTGGT CCAAGTAAAC 
AAAAACGCCA ACCCAGACCC ACTGCCAAAG AAAGAGGAAG AGAAGAAGGA GGAAGAGGAG 
GCAGACCCCG GGGAGGATGG CCCAAAGCCC ATGCCGCCCT ACAGCTCCAT GTTCATCCTC 
TCCACCACCA ACCCCCTTCG CCGGCTGTGC CATTACATCC TGAACCTGCG CTACTTCGAG 
ATGTGCATCC TCATGGTCAT TGCCATGAGT AGCATCGCGC TGGCCGCCGA GGACCCGGTG 
CAGCCCAACG CACCCCGCAA CAACGTGCTG CGATATTTTG ACTATGTTTT CACAGGAGTG 
TTTACCTTTG AGATGGTGAT CAAGATGATC GACCTGGGCC TCGTCCTGCA TCAGGGGGCC 
TATTTCCGTG ACCTGTGGAA CATTCTGGAC TTCATAGTGG TCAGTGGGGC CCTGGTGGCC 
TTTGCCTTCA CGAGCAGTAC ACGTGGCAAT AGCAAAGGAA AGGACATCAA CACCATCAAG 
TCCCTCCGAG TCCTCCGGGT GCTACGACCT CTAAAGACCA TCAAGCGGCT GCCTAAGTTG 
AAGGCCGTAT TTGACTGCGT GGTGAACTCG CTCAAGAACG TCTTCAACAT CCTCATTGTC 
TACATGCTCT TCATGTTCAT CTTCGCCGTG GTGGCCGTGC AGCTCTTCAA GGGCAAATTC 
TTCCACTGCA CGGACGAGTC CAAGGAGTTT GAGAGAGACT GTCGAGGCAA ATACCTCCTT 
TACGAGAAGA ACGAGGTAAA GGCGCGGGAC CGCGAGTGGA AGAAATACGA CTTCCACTAC 
GACAACGTGC TCTGGGCCCT GCTCACGCTC TTTACGGTGT CCACGGGAGA GGGCTGGCCA 
CAGGTCCTCA AGCACTCAGT GGATGCCACT TTTGAGAACC AGGGCCCCAG CCCCGGGTAC 
CGCATGGAAA TGTCCATCTT CTACGTGGTC TACTTTGTGG TGTTTCCCTT CTTCTTTGTC 
AATATCTTTG TGGCCTTGAT CATCATCACC TTCCAGGAGC AGGGAGACAA GATGATGGAA 
GAATACAGCC TAGAGAAAAA TGAGAGGGCC TGCATCGACT TTGCCATCAG TGCCAAGCCG 
CTGACCAGGC ACATGCCCCA GAACAAGCAG AGCTTCCAGT ATCGAATGTG GCAGTTCGTG 
GTGTCCCCAC CCTTTGAGTA CACCATCATG GCCATGATCG CTCTCAACAC CATCGTGCTA 
ATGATGAAGT TCTATGGAGC CTCTGTGGCC TATGAAAACG CCCTTCGAGT GTTCAACATT 
GTCTTCACCT CCCTCTTCTC TCTCGAATGT GTGCTCAAAG TCATGGCTTT TGGGATTCTG 
AATTATTTCC GCGATGCCTG GAACATCTTC GACTTTGTGA CTGTTCTGGG CAGCATCACA 
GACATCCTCG TCACCGAGTT TGGGAATAAC TTCATCAACC TGAGCTTTCT CCGCCTCTTC 
CGTGCTGCCC GACTCATCAA ACTCCTCCGC CAGGGTTACA CCATCCGCAT TCTCCTCTGG 
ACTTTCGTGC AGTCTTTCAA GGCCCTACCT TATGTCTGTC TGCTGATCGC CATGCTCTTC 
TTCATCTATG CCATCATCGG GATGCAGGTG TTTGGCAACA TCGGCATTGA TGGGGAAGAT 
GAGGACAGCG ATGAGGATGA GTTCCAAATC ACGGAGCACA ATAACTTCCG GACCTTCTTC 
CAAGCTCTCA TGCTTCTCTT CCGGAGCGCC ACAGGGGAAG CGTGGCACAA CATCATGCTG 
TCCTGCCTCA GCGGGAAGCC ATGCGACAAG AACTCCGGGA TCCAAAAACC AGAGTGTGGC 
AACGAGTTCG CCTATTTTTA CTTTGTCTCG TTCATCTTCC TTTGCTCATT TCTGATGCTG 
AATCTCTTTG TTGCTGTCAT CATGGACAAC TTCGAGTACC TCACCCGAGA TTCCTCCATC 
CTGGGCCCCC ACCACCTGGA TGAGTACGTG CGTGTCTGGG CAGAGTATGA CCCTGCTGCC 
TGCGGCCGCA TTCACTATAA GGACATGTAC AGTTTATTGC GAGTAATATC GCCCCCTCTC 
GGCTTAGGCA AGAAATGTCC TCATAGGGTT GCTTGCAAGA GGCTCTTGCG GATGGACCTA 
CCCGTAGCGG ATGACAACAC CGTTCACTTC AACTCCACCT TGATGGCTCT GATCCGAACC 
GCCCTGGATA TCAAAATCGC AAAGGGTGGA GCTGACAAGC AGCAAATGGA CGCAGAGCTC 
CGCAAGGAAA TGATGGCCAT TTGGCCCAAC CTGTCTCAGA AGACCTTGGA TCTGCTGGTC 
ACACCTCACA AGTCCACGGA CCTGACAGTG GGTAAGATCT ACGCAGCCAT GATGATCATG 
GAGTACTACC GGCAGAGCAA GGCCAAGAAG CTGCAGGCCA TGCGAGAGGA GCAGAACCGG 
ACACCACTCA TGTTCCAGCG CATGGAGCCT CCATCGCCAA CACAGGAGGG AGGACCCAGC 
CAAAACGCCC TTCCCTCCAC TCAGCTGGAC CCCGGAGGAG GCCTGATGGC TCAAGAAAGC 
AGCATGAAGG AGAGCCCGTC CTGGGTGACC CAGCGGGCAC AGGAGATGTT CCAGAAGACT 
GGTACCTGGA GCCCAGAGCG AGGGCCACCC ATCGACATGC CTAACAGCCA GCCCAACTCC 
CAGTCTGTGG AGATGAGAGA AATGGGAACT GATGGCTACT CAGACAGCGA ACACTACCTC 
CCCATGGAAG GACAGACCAG GGCCGCCTCC ATGCCCCGCC TCCCAGCAGA GAACCAGAGG 
AGAAGGGGCC GGCCACGTGG AAATAACCTC AGTACCATCT CTGATACCAG CCCCATGAAG 
CGCTCAGCCT CCGTGCTGGG ACCCAAAGCC CGGCGACTGG ATGACTACTC ACTAGAGCGG 
GTACCACCTG AGGAGAACCA AAGGTACCAC CAACGCCGCC GGGACCGTGG CCACCGCACC 
TCTGAGCGCT CTCTGGGCCG ATACACTGAT GTGGACACAG GCCTGGGGAC AGATCTGAGC 
ATGACCACCC AATCGGGTGA CCTGCCCTCC AAAGATCGGG ACCAGGACCG GGGCCGGCCC 
AAGGACCGGA AGCATCGGCC ACACCACCAC CACCACCATC ATCACCATCA TCCCCCGGCC 
CCGGACCGGG AGCGCTACGC ACAGGAGCGG CCGGACACCG GCCGGGCGCG GGCCCGGGAG 
CAGCGCTGGT CCCGCTCGCC CAGCGAGGGT CGGGAGCACG CGACACACAG ACAGGGCAGT 
AGTTCCGTCA GTGGAAGCCC AGCCCCCTCT ACGTCCGGCA CCAGCACGCC GCGGCGCGGC 
CGCCGCCAGC TCCCCCAGAC CCCCTGCACC CCGCGGCCGC TTGTGTCCTA CTCGCCCGCT 
CCGCGCAGGC CTGCGGCGCG CAGGATGGCG GGTCCCCCAG CGCCCCCTGG CGGCTCGCCG 
CGGGGTTGCC GCCGTGCGCC GCGCTGGCCT GCGCATGCGC CTGAGGGCCC GCGGCCGCGC 
GGTGCCGACT ACACCGAGCC CGACAGCCCG CGCGAGCCGC CAGGGGGCGC GCATGAGCCC 
GCGCCGCGGT CGCCCAGGAC TCCGCGCGCC GCGGGCTGCG CGTCACCGCG CCACGGCCGC 
AGGCTGCCCA ATGGTTACTA CGCGGGGCAC GGCGCGCCAC GGCCGCGCAC AGCCCGCAGG 
GGCGCGCACG ACGCGTACAG CGAGAGCGAG GACGACTGGT GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017456668.1
CDS	105..7529
Translation	MVSSPGISERGPWMPRASWVSDPMRSPLNHELLHSSRYPGTDCTLAGSSRTLHVPWSEQMSGSLEGPTLKRRGGVVDHRDVILAHQAHKMHSTPQARRKEWEMARFGDEMPGRYGAGGGGSGPAAGVVVGAAGGRGAGGSRQGGQPGAQRMYKQSMAQRARTMALYNPIPVRQNCLTVNRSLFLFSEDNVVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDDDKTPMSERLDDTEPYFIGIFCFEAGIKIVALGFAFHKGSYLRNGWNVMDFVVVLTGILATVGTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILIFAIIGLEFYMGKFHTTCFEEGTDDIQGESPAPCGTEEPARTCPNGTKCQPYWEGPNNGITQFDNILFAVLTVFQCITMEGWTDLLYNSNDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYMEWISKAEEVILAEDETDVEQRHPFDGALRRATLKKSKTDLLNPEEAEDQLADIASVGSPFARASIKSAKLENSTFFHKKERRMRFYIRRMVKTQAFYWTVLSLVALNTLCVAIVHYNQPEWLSDFLYYAEFIFLGLFMSEMFIKMYGLGTRPYFHSSFNCFDCGVIIGSIFEVIWAVIKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFDEGTPPTNFDTFPAAIMTVFQILTGEDWNEVMYDGIKSQGGVQGGMVFSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAANQKLALQKAKEVAEVSPLSAANMSIAVKEQQKNQKPAKSVWEQRTSEMRKQNLLASREALYGDAAERWPTTYARPLRPDVKTHLDRPLVVDPQENRNNNTNKSRAPEALRQTARPRESARDPDARRAWPSSPERAPGREGPYGRESEPQQREHAPPREHVPWDADPERAKAGDAPRRHTHRPVAEGEPRRHRARRRPGDEPDDRPERRPRPRDATRPARAADGEGDDGERKRRHRHGPPAHDDRERRHRRRKESQGSGVPMSGPNLSTTRPIQQDLGRQDLPLAEDLDNMKNNKLATGEPASPHDSLGHSGLPPSPAKIGNSTNPGPALATNPQNAASRRTPNNPGNPSNPGPPKTPENSLIVTNPSSTQPNSAKTARKPEHMAVEIPPACPPLNHTVVQVNKNANPDPLPKKEEEKKEEEEADPGEDGPKPMPPYSSMFILSTTNPLRRLCHYILNLRYFEMCILMVIAMSSIALAAEDPVQPNAPRNNVLRYFDYVFTGVFTFEMVIKMIDLGLVLHQGAYFRDLWNILDFIVVSGALVAFAFTSSTRGNSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYMLFMFIFAVVAVQLFKGKFFHCTDESKEFERDCRGKYLLYEKNEVKARDREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATFENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEEYSLEKNERACIDFAISAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYTIMAMIALNTIVLMMKFYGASVAYENALRVFNIVFTSLFSLECVLKVMAFGILNYFRDAWNIFDFVTVLGSITDILVTEFGNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIGIDGEDEDSDEDEFQITEHNNFRTFFQALMLLFRSATGEAWHNIMLSCLSGKPCDKNSGIQKPECGNEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRVWAEYDPAACGRIHYKDMYSLLRVISPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKQQMDAELRKEMMAIWPNLSQKTLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKKLQAMREEQNRTPLMFQRMEPPSPTQEGGPSQNALPSTQLDPGGGLMAQESSMKESPSWVTQRAQEMFQKTGTWSPERGPPIDMPNSQPNSQSVEMREMGTDGYSDSEHYLPMEGQTRAASMPRLPAENQRRRGRPRGNNLSTISDTSPMKRSASVLGPKARRLDDYSLERVPPEENQRYHQRRRDRGHRTSERSLGRYTDVDTGLGTDLSMTTQSGDLPSKDRDQDRGRPKDRKHRPHHHHHHHHHHPPAPDRERYAQERPDTGRARAREQRWSRSPSEGREHATHRQGSSSVSGSPAPSTSGTSTPRRGRRQLPQTPCTPRPLVSYSPAPRRPAARRMAGPPAPPGGSPRGCRRAPRWPAHAPEGPRPRGADYTEPDSPREPPGGAHEPAPRSPRTPRAAGCASPRHGRRLPNGYYAGHGAPRPRTARRGAHDAYSESEDDWC

Target ORF information:

RefSeq Version	XM_017601179.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel subunit alpha1 A (Cacna1a), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017601179.1

ORF Insert Sequence:

ATGGTTTCCT CTCCTGGGAT TTCTGAGCGA GGCCCATGGA TGCCGAGGGC CTCCTGGGTC 
TCTGACCCCA TGCGTTCTCC CCTCAATCAT GAGCTCCTGC ACAGCTCGAG GTACCCAGGC 
ACGGACTGCA CCCTGGCAGG TTCTTCCAGG ACTTTGCATG TCCCGTGGTC CGAGCAGATG 
AGCGGAAGCC TCGAGGGGCC AACTCTGAAA CGGCGTGGTG GCGTGGTGGA CCACCGGGAT 
GTCATCTTGG CCCACCAGGC GCACAAAATG CACAGCACCC CTCAGGCCAG GAGGAAAGAG 
TGGGAAATGG CCCGCTTTGG AGACGAGATG CCGGGCCGCT ACGGCGCAGG CGGAGGAGGC 
TCAGGGCCGG CCGCCGGGGT GGTCGTGGGC GCCGCGGGCG GCCGAGGAGC CGGGGGCAGC 
CGGCAGGGCG GGCAGCCCGG AGCGCAGAGG ATGTACAAGC AGTCGATGGC GCAGAGAGCG 
CGGACCATGG CCCTCTACAA CCCCATCCCT GTCCGCCAGA ACTGCCTCAC GGTCAACCGC 
TCCCTGTTCC TCTTCAGTGA AGACAACGTG GTGAGAAAAT ACGCCAAAAA GATCACGGAA 
TGGCCTCCCT TCGAGTACAT GATCCTGGCC ACCATCATTG CTAACTGCAT CGTCCTGGCC 
CTGGAGCAGC ACCTCCCTGA TGATGACAAG ACACCCATGT CCGAGCGGCT GGATGACACA 
GAACCCTATT TCATTGGCAT CTTCTGTTTT GAGGCTGGAA TTAAGATCGT GGCTCTTGGC 
TTTGCCTTCC ACAAAGGCTC CTACCTGAGG AATGGCTGGA ACGTCATGGA CTTTGTCGTG 
GTGCTAACAG GCATCTTGGC CACTGTCGGG ACGGAGTTTG ATCTACGGAC ACTGAGGGCG 
GTTCGTGTGC TGCGGCCACT CAAGCTGGTG TCTGGAATCC CAAGTTTACA AGTCGTCCTG 
AAGTCAATCA TGAAGGCGAT GATCCCTCTG CTGCAGATCG GCCTCCTCCT GTTTTTTGCA 
ATCCTTATTT TTGCAATCAT AGGGTTAGAA TTTTATATGG GAAAATTTCA TACCACCTGC 
TTTGAAGAGG GGACAGACGA CATCCAGGGT GAGTCGCCAG CTCCGTGTGG GACAGAGGAG 
CCTGCCCGCA CCTGCCCCAA CGGGACCAAA TGTCAGCCGT ACTGGGAAGG GCCCAACAAC 
GGCATCACTC AGTTCGACAA CATCCTGTTT GCTGTGCTCA CTGTTTTCCA GTGCATCACC 
ATGGAAGGCT GGACTGATCT CCTCTACAAT AGCAACGATG CCTCAGGGAA CACTTGGAAC 
TGGTTGTACT TCATCCCCCT CATCATCATC GGCTCCTTTT TTATGCTGAA CCTTGTGCTG 
GGTGTGCTGT CTGGGGAGTT TGCCAAAGAA AGGGAACGTG TAGAGAACCG AAGGGCTTTT 
CTGAAGCTCA GAAGACAACA GCAGATTGAA CGTGAGCTCA ATGGATACAT GGAGTGGATC 
TCGAAAGCAG AAGAGGTGAT CCTCGCGGAG GACGAGACAG ACGTGGAGCA GAGGCACCCT 
TTTGATGGAG CTCTTCGGAG AGCTACTCTG AAGAAAAGCA AGACGGACCT GCTCAACCCT 
GAGGAGGCGG AGGACCAGCT TGCTGACATC GCGTCTGTGG GGTCTCCCTT CGCCAGAGCC 
AGCATCAAAA GTGCCAAGCT GGAGAATTCG ACTTTTTTCC ACAAAAAGGA GAGAAGAATG 
CGTTTCTACA TCCGCCGCAT GGTCAAAACT CAGGCCTTCT ACTGGACCGT GCTCAGTCTG 
GTAGCCCTCA ACACGCTGTG CGTCGCCATT GTCCACTACA ACCAGCCCGA GTGGCTCTCC 
GACTTCCTCT ACTATGCAGA ATTCATTTTC TTAGGACTCT TTATGTCCGA AATGTTTATA 
AAAATGTATG GGCTCGGGAC ACGGCCTTAC TTCCACTCTT CCTTCAACTG CTTTGACTGT 
GGGGTCATCA TCGGGAGCAT CTTTGAAGTC ATCTGGGCCG TCATCAAACC GGGTACATCC 
TTTGGAATCA GCGTGTTACG AGCTCTCAGG TTACTGCGTA TTTTCAAAGT CACAAAGTAC 
TGGGCATCTC TCAGAAACCT GGTTGTCTCC CTCCTCAACT CCATGAAATC CATCATAAGT 
CTGCTGTTCC TCCTCTTCCT CTTCATTGTC GTCTTTGCCC TCTTGGGGAT GCAGCTGTTT 
GGTGGCCAGT TTAATTTTGA CGAGGGGACT CCTCCCACCA ACTTCGACAC TTTTCCAGCA 
GCAATAATGA CTGTGTTTCA GATCCTGACT GGCGAGGATT GGAATGAGGT CATGTATGAT 
GGGATCAAGT CTCAGGGGGG CGTGCAGGGC GGCATGGTGT TCTCCATCTA CTTCATCGTC 
CTCACCCTCT TCGGGAACTA CACCCTGCTG AACGTGTTCT TGGCTATCGC GGTGGACAAC 
CTGGCCAACG CCCAGGAACT CACCAAGGAT GAACAAGAAG AGGAAGAGGC AGCCAATCAG 
AAACTGGCTC TACAGAAAGC CAAGGAGGTG GCAGAAGTGA GTCCCCTGTC TGCAGCCAAC 
ATGTCCATAG CTGTGAAGGA ACAGCAGAAG AACCAGAAGC CTGCCAAGTC GGTGTGGGAG 
CAGCGCACCA GCGAGATGCG CAAGCAGAAC CTGCTGGCTA GCCGCGAGGC GCTGTACGGG 
GACGCGGCTG AGCGCTGGCC CACCACTTAC GCGCGCCCGC TGCGGCCGGA CGTGAAGACG 
CACTTGGACC GGCCGCTCGT GGTGGACCCG CAGGAGAACC GTAACAACAA CACCAACAAG 
AGCCGTGCGC CAGAAGCGCT GCGCCAAACC GCGCGGCCCC GCGAGAGCGC GCGCGACCCC 
GACGCGCGGC GCGCCTGGCC CAGCAGCCCT GAGCGCGCCC CTGGACGAGA GGGCCCGTAT 
GGCCGCGAGA GCGAGCCGCA ACAGCGCGAG CACGCGCCAC CCCGCGAGCA CGTACCCTGG 
GACGCGGATC CTGAGCGCGC CAAGGCCGGG GACGCGCCCC GCCGCCACAC GCACCGGCCT 
GTGGCCGAGG GCGAGCCTCG TCGCCACCGC GCGCGCCGCC GGCCCGGGGA CGAACCGGAC 
GACAGACCGG AGCGCAGGCC GCGTCCCCGC GACGCCACTA GGCCGGCCCG CGCTGCAGAC 
GGCGAAGGCG ATGATGGGGA GCGCAAGCGG CGACACCGAC ACGGGCCGCC GGCCCACGAT 
GACAGGGAGC GCAGACACCG GCGCAGAAAA GAGAGCCAGG GCTCTGGGGT CCCCATGTCT 
GGTCCCAACC TGTCCACCAC CAGGCCAATC CAGCAGGATC TGGGCCGCCA GGACCTGCCA 
CTGGCTGAGG ACCTGGACAA CATGAAGAAC AACAAGTTGG CCACCGGGGA GCCTGCCAGT 
CCCCACGACA GCCTGGGCCA CAGTGGCCTT CCCCCTAGCC CTGCCAAGAT CGGGAACAGC 
ACCAACCCTG GTCCCGCCTT GGCCACCAAT CCCCAGAATG CTGCCAGCCG CAGGACGCCC 
AACAACCCGG GCAACCCGTC CAACCCCGGC CCCCCCAAGA CTCCCGAGAA CAGCCTTATC 
GTCACCAACC CCAGCAGCAC CCAGCCCAAC TCAGCAAAGA CTGCCAGGAA ACCCGAGCAC 
ATGGCGGTGG AGATCCCCCC GGCCTGCCCG CCCCTCAACC ACACTGTGGT CCAAGTAAAC 
AAAAACGCCA ACCCAGACCC ACTGCCAAAG AAAGAGGAAG AGAAGAAGGA GGAAGAGGAG 
GCAGACCCCG GGGAGGATGG CCCAAAGCCC ATGCCGCCCT ACAGCTCCAT GTTCATCCTC 
TCCACCACCA ACCCCCTTCG CCGGCTGTGC CATTACATCC TGAACCTGCG CTACTTCGAG 
ATGTGCATCC TCATGGTCAT TGCCATGAGT AGCATCGCGC TGGCCGCCGA GGACCCGGTG 
CAGCCCAACG CACCCCGCAA CAACGTGCTG CGATATTTTG ACTATGTTTT CACAGGAGTG 
TTTACCTTTG AGATGGTGAT CAAGATGATC GACCTGGGCC TCGTCCTGCA TCAGGGGGCC 
TATTTCCGTG ACCTGTGGAA CATTCTGGAC TTCATAGTGG TCAGTGGGGC CCTGGTGGCC 
TTTGCCTTCA CGAGCAGTAC ACGTGGCAAT AGCAAAGGAA AGGACATCAA CACCATCAAG 
TCCCTCCGAG TCCTCCGGGT GCTACGACCT CTAAAGACCA TCAAGCGGCT GCCTAAGTTG 
AAGGCCGTAT TTGACTGCGT GGTGAACTCG CTCAAGAACG TCTTCAACAT CCTCATTGTC 
TACATGCTCT TCATGTTCAT CTTCGCCGTG GTGGCCGTGC AGCTCTTCAA GGGCAAATTC 
TTCCACTGCA CGGACGAGTC CAAGGAGTTT GAGAGAGACT GTCGAGGCAA ATACCTCCTT 
TACGAGAAGA ACGAGGTAAA GGCGCGGGAC CGCGAGTGGA AGAAATACGA CTTCCACTAC 
GACAACGTGC TCTGGGCCCT GCTCACGCTC TTTACGGTGT CCACGGGAGA GGGCTGGCCA 
CAGGTCCTCA AGCACTCAGT GGATGCCACT TTTGAGAACC AGGGCCCCAG CCCCGGGTAC 
CGCATGGAAA TGTCCATCTT CTACGTGGTC TACTTTGTGG TGTTTCCCTT CTTCTTTGTC 
AATATCTTTG TGGCCTTGAT CATCATCACC TTCCAGGAGC AGGGAGACAA GATGATGGAA 
GAATACAGCC TAGAGAAAAA TGAGAGGGCC TGCATCGACT TTGCCATCAG TGCCAAGCCG 
CTGACCAGGC ACATGCCCCA GAACAAGCAG AGCTTCCAGT ATCGAATGTG GCAGTTCGTG 
GTGTCCCCAC CCTTTGAGTA CACCATCATG GCCATGATCG CTCTCAACAC CATCGTGCTA 
ATGATGAAGT TCTATGGAGC CTCTGTGGCC TATGAAAACG CCCTTCGAGT GTTCAACATT 
GTCTTCACCT CCCTCTTCTC TCTCGAATGT GTGCTCAAAG TCATGGCTTT TGGGATTCTG 
AATTATTTCC GCGATGCCTG GAACATCTTC GACTTTGTGA CTGTTCTGGG CAGCATCACA 
GACATCCTCG TCACCGAGTT TGGGAATAAC TTCATCAACC TGAGCTTTCT CCGCCTCTTC 
CGTGCTGCCC GACTCATCAA ACTCCTCCGC CAGGGTTACA CCATCCGCAT TCTCCTCTGG 
ACTTTCGTGC AGTCTTTCAA GGCCCTACCT TATGTCTGTC TGCTGATCGC CATGCTCTTC 
TTCATCTATG CCATCATCGG GATGCAGGTG TTTGGCAACA TCGGCATTGA TGGGGAAGAT 
GAGGACAGCG ATGAGGATGA GTTCCAAATC ACGGAGCACA ATAACTTCCG GACCTTCTTC 
CAAGCTCTCA TGCTTCTCTT CCGGAGCGCC ACAGGGGAAG CGTGGCACAA CATCATGCTG 
TCCTGCCTCA GCGGGAAGCC ATGCGACAAG AACTCCGGGA TCCAAAAACC AGAGTGTGGC 
AACGAGTTCG CCTATTTTTA CTTTGTCTCG TTCATCTTCC TTTGCTCATT TCTGATGCTG 
AATCTCTTTG TTGCTGTCAT CATGGACAAC TTCGAGTACC TCACCCGAGA TTCCTCCATC 
CTGGGCCCCC ACCACCTGGA TGAGTACGTG CGTGTCTGGG CAGAGTATGA CCCTGCTGCC 
TGCGGCCGCA TTCACTATAA GGACATGTAC AGTTTATTGC GAGTAATATC GCCCCCTCTC 
GGCTTAGGCA AGAAATGTCC TCATAGGGTT GCTTGCAAGA GGCTCTTGCG GATGGACCTA 
CCCGTAGCGG ATGACAACAC CGTTCACTTC AACTCCACCT TGATGGCTCT GATCCGAACC 
GCCCTGGATA TCAAAATCGC AAAGGGTGGA GCTGACAAGC AGCAAATGGA CGCAGAGCTC 
CGCAAGGAAA TGATGGCCAT TTGGCCCAAC CTGTCTCAGA AGACCTTGGA TCTGCTGGTC 
ACACCTCACA AGTCCACGGA CCTGACAGTG GGTAAGATCT ACGCAGCCAT GATGATCATG 
GAGTACTACC GGCAGAGCAA GGCCAAGAAG CTGCAGGCCA TGCGAGAGGA GCAGAACCGG 
ACACCACTCA TGTTCCAGCG CATGGAGCCT CCATCGCCAA CACAGGAGGG AGGACCCAGC 
CAAAACGCCC TTCCCTCCAC TCAGCTGGAC CCCGGAGGAG GCCTGATGGC TCAAGAAAGC 
AGCATGAAGG AGAGCCCGTC CTGGGTGACC CAGCGGGCAC AGGAGATGTT CCAGAAGACT 
GGTACCTGGA GCCCAGAGCG AGGGCCACCC ATCGACATGC CTAACAGCCA GCCCAACTCC 
CAGTCTGTGG AGATGAGAGA AATGGGAACT GATGGCTACT CAGACAGCGA ACACTACCTC 
CCCATGGAAG GACAGACCAG GGCCGCCTCC ATGCCCCGCC TCCCAGCAGA GAACCAGAGG 
AGAAGGGGCC GGCCACGTGG AAATAACCTC AGTACCATCT CTGATACCAG CCCCATGAAG 
CGCTCAGCCT CCGTGCTGGG ACCCAAAGCC CGGCGACTGG ATGACTACTC ACTAGAGCGG 
GTACCACCTG AGGAGAACCA AAGGTACCAC CAACGCCGCC GGGACCGTGG CCACCGCACC 
TCTGAGCGCT CTCTGGGCCG ATACACTGAT GTGGACACAG GCCTGGGGAC AGATCTGAGC 
ATGACCACCC AATCGGGTGA CCTGCCCTCC AAAGATCGGG ACCAGGACCG GGGCCGGCCC 
AAGGACCGGA AGCATCGGCC ACACCACCAC CACCACCATC ATCACCATCA TCCCCCGGCC 
CCGGACCGGG AGCGCTACGC ACAGGAGCGG CCGGACACCG GCCGGGCGCG GGCCCGGGAG 
CAGCGCTGGT CCCGCTCGCC CAGCGAGGGT CGGGAGCACG CGACACACAG ACAGGGCAGT 
AGTTCCGTCA GTGGAAGCCC AGCCCCCTCT ACGTCCGGCA CCAGCACGCC GCGGCGCGGC 
CGCCGCCAGC TCCCCCAGAC CCCCTGCACC CCGCGGCCGC TTGTGTCCTA CTCGCCCGCT 
CCGCGCAGGC CTGCGGCGCG CAGGATGGCG GGTCCCCCAG CGCCCCCTGG CGGCTCGCCG 
CGGGGTTGCC GCCGTGCGCC GCGCTGGCCT GCGCATGCGC CTGAGGGCCC GCGGCCGCGC 
GGTGCCGACT ACACCGAGCC CGACAGCCCG CGCGAGCCGC CAGGGGGCGC GCATGAGCCC 
GCGCCGCGGT CGCCCAGGAC TCCGCGCGCC GCGGGCTGCG CGTCACCGCG CCACGGCCGC 
AGGCTGCCCA ATGGTTACTA CGCGGGGCAC GGCGCGCCAC GGCCGCGCAC AGCCCGCAGG 
GGCGCGCACG ACGCGTACAG CGAGAGCGAG GACGACTGGT GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1a ( XM_017601180.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017456669.1 Rattus norvegicus calcium voltage-gated channel subunit alpha1 A (Cacna1a), transcript variant X11, mRNA.

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CloneID	ORa59374
Clone ID Related Accession (Same CDS sequence)	XM_017601180.1
Accession Version	XM_017601180.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7425bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTCCT CTCCTGGGAT TTCTGAGCGA GGCCCATGGA TGCCGAGGGC CTCCTGGGTC 
TCTGACCCCA TGCGTTCTCC CCTCAATCAT GAGCTCCTGC ACAGCTCGAG GTACCCAGGC 
ACGGACTGCA CCCTGGCAGG TTCTTCCAGG ACTTTGCATG TCCCGTGGTC CGAGCAGATG 
AGCGGAAGCC TCGAGGGGCC AACTCTGAAA CGGCGTGGTG GCGTGGTGGA CCACCGGGAT 
GTCATCTTGG CCCACCAGGC GCACAAAATG CACAGCACCC CTCAGGCCAG GAGGAAAGAG 
TGGGAAATGG CCCGCTTTGG AGACGAGATG CCGGGCCGCT ACGGCGCAGG CGGAGGAGGC 
TCAGGGCCGG CCGCCGGGGT GGTCGTGGGC GCCGCGGGCG GCCGAGGAGC CGGGGGCAGC 
CGGCAGGGCG GGCAGCCCGG AGCGCAGAGG ATGTACAAGC AGTCGATGGC GCAGAGAGCG 
CGGACCATGG CCCTCTACAA CCCCATCCCT GTCCGCCAGA ACTGCCTCAC GGTCAACCGC 
TCCCTGTTCC TCTTCAGTGA AGACAACGTG GTGAGAAAAT ACGCCAAAAA GATCACGGAA 
TGGCCTCCCT TCGAGTACAT GATCCTGGCC ACCATCATTG CTAACTGCAT CGTCCTGGCC 
CTGGAGCAGC ACCTCCCTGA TGATGACAAG ACACCCATGT CCGAGCGGCT GGATGACACA 
GAACCCTATT TCATTGGCAT CTTCTGTTTT GAGGCTGGAA TTAAGATCGT GGCTCTTGGC 
TTTGCCTTCC ACAAAGGCTC CTACCTGAGG AATGGCTGGA ACGTCATGGA CTTTGTCGTG 
GTGCTAACAG GCATCTTGGC CACTGTCGGG ACGGAGTTTG ATCTACGGAC ACTGAGGGCG 
GTTCGTGTGC TGCGGCCACT CAAGCTGGTG TCTGGAATCC CAAGTTTACA AGTCGTCCTG 
AAGTCAATCA TGAAGGCGAT GATCCCTCTG CTGCAGATCG GCCTCCTCCT GTTTTTTGCA 
ATCCTTATTT TTGCAATCAT AGGGTTAGAA TTTTATATGG GAAAATTTCA TACCACCTGC 
TTTGAAGAGG GGACAGACGA CATCCAGGGT GAGTCGCCAG CTCCGTGTGG GACAGAGGAG 
CCTGCCCGCA CCTGCCCCAA CGGGACCAAA TGTCAGCCGT ACTGGGAAGG GCCCAACAAC 
GGCATCACTC AGTTCGACAA CATCCTGTTT GCTGTGCTCA CTGTTTTCCA GTGCATCACC 
ATGGAAGGCT GGACTGATCT CCTCTACAAT AGCAACGATG CCTCAGGGAA CACTTGGAAC 
TGGTTGTACT TCATCCCCCT CATCATCATC GGCTCCTTTT TTATGCTGAA CCTTGTGCTG 
GGTGTGCTGT CTGGGGAGTT TGCCAAAGAA AGGGAACGTG TAGAGAACCG AAGGGCTTTT 
CTGAAGCTCA GAAGACAACA GCAGATTGAA CGTGAGCTCA ATGGATACAT GGAGTGGATC 
TCGAAAGCAG AAGAGGTGAT CCTCGCGGAG GACGAGACAG ACGTGGAGCA GAGGCACCCT 
TTTGATGGAG CTCTTCGGAG AGCTACTCTG AAGAAAAGCA AGACGGACCT GCTCAACCCT 
GAGGAGGCGG AGGACCAGCT TGCTGACATC GCGTCTGTGG GGTCTCCCTT CGCCAGAGCC 
AGCATCAAAA GTGCCAAGCT GGAGAATTCG ACTTTTTTCC ACAAAAAGGA GAGAAGAATG 
CGTTTCTACA TCCGCCGCAT GGTCAAAACT CAGGCCTTCT ACTGGACCGT GCTCAGTCTG 
GTAGCCCTCA ACACGCTGTG CGTCGCCATT GTCCACTACA ACCAGCCCGA GTGGCTCTCC 
GACTTCCTCT ACTATGCAGA ATTCATTTTC TTAGGACTCT TTATGTCCGA AATGTTTATA 
AAAATGTATG GGCTCGGGAC ACGGCCTTAC TTCCACTCTT CCTTCAACTG CTTTGACTGT 
GGGGTCATCA TCGGGAGCAT CTTTGAAGTC ATCTGGGCCG TCATCAAACC GGGTACATCC 
TTTGGAATCA GCGTGTTACG AGCTCTCAGG TTACTGCGTA TTTTCAAAGT CACAAAGTAC 
TGGGCATCTC TCAGAAACCT GGTTGTCTCC CTCCTCAACT CCATGAAATC CATCATAAGT 
CTGCTGTTCC TCCTCTTCCT CTTCATTGTC GTCTTTGCCC TCTTGGGGAT GCAGCTGTTT 
GGTGGCCAGT TTAATTTTGA CGAGGGGACT CCTCCCACCA ACTTCGACAC TTTTCCAGCA 
GCAATAATGA CTGTGTTTCA GATCCTGACT GGCGAGGATT GGAATGAGGT CATGTATGAT 
GGGATCAAGT CTCAGGGGGG CGTGCAGGGC GGCATGGTGT TCTCCATCTA CTTCATCGTC 
CTCACCCTCT TCGGGAACTA CACCCTGCTG AACGTGTTCT TGGCTATCGC GGTGGACAAC 
CTGGCCAACG CCCAGGAACT CACCAAGGAT GAACAAGAAG AGGAAGAGGC AGCCAATCAG 
AAACTGGCTC TACAGAAAGC CAAGGAGGTG GCAGAAGTGA GTCCCCTGTC TGCAGCCAAC 
ATGTCCATAG CTGTGAAGGA ACAGCAGAAG AACCAGAAGC CTGCCAAGTC GGTGTGGGAG 
CAGCGCACCA GCGAGATGCG CAAGCAGAAC CTGCTGGCTA GCCGCGAGGC GCTGTACGGG 
GACGCGGCTG AGCGCTGGCC CACCACTTAC GCGCGCCCGC TGCGGCCGGA CGTGAAGACG 
CACTTGGACC GGCCGCTCGT GGTGGACCCG CAGGAGAACC GTAACAACAA CACCAACAAG 
AGCCGTGCGC CAGAAGCGCT GCGCCAAACC GCGCGGCCCC GCGAGAGCGC GCGCGACCCC 
GACGCGCGGC GCGCCTGGCC CAGCAGCCCT GAGCGCGCCC CTGGACGAGA GGGCCCGTAT 
GGCCGCGAGA GCGAGCCGCA ACAGCGCGAG CACGCGCCAC CCCGCGAGCA CGTACCCTGG 
GACGCGGATC CTGAGCGCGC CAAGGCCGGG GACGCGCCCC GCCGCCACAC GCACCGGCCT 
GTGGCCGAGG GCGAGCCTCG TCGCCACCGC GCGCGCCGCC GGCCCGGGGA CGAACCGGAC 
GACAGACCGG AGCGCAGGCC GCGTCCCCGC GACGCCACTA GGCCGGCCCG CGCTGCAGAC 
GGCGAAGGCG ATGATGGGGA GCGCAAGCGG CGACACCGAC ACGGGCCGCC GGCCCACGAT 
GACAGGGAGC GCAGACACCG GCGCAGAAAA GAGAGCCAGG GCTCTGGGGT CCCCATGTCT 
GGTCCCAACC TGTCCACCAC CAGGCCAATC CAGCAGGATC TGGGCCGCCA GGACCTGCCA 
CTGGCTGAGG ACCTGGACAA CATGAAGAAC AACAAGTTGG CCACCGGGGA GCCTGCCAGT 
CCCCACGACA GCCTGGGCCA CAGTGGCCTT CCCCCTAGCC CTGCCAAGAT CGGGAACAGC 
ACCAACCCTG GTCCCGCCTT GGCCACCAAT CCCCAGAATG CTGCCAGCCG CAGGACGCCC 
AACAACCCGG GCAACCCGTC CAACCCCGGC CCCCCCAAGA CTCCCGAGAA CAGCCTTATC 
GTCACCAACC CCAGCAGCAC CCAGCCCAAC TCAGCAAAGA CTGCCAGGAA ACCCGAGCAC 
ATGGCGGTGG AGATCCCCCC GGCCTGCCCG CCCCTCAACC ACACTGTGGT CCAAGTAAAC 
AAAAACGCCA ACCCAGACCC ACTGCCAAAG AAAGAGGAAG AGAAGAAGGA GGAAGAGGAG 
GCAGACCCCG GGGAGGATGG CCCAAAGCCC ATGCCGCCCT ACAGCTCCAT GTTCATCCTC 
TCCACCACCA ACCCCCTTCG CCGGCTGTGC CATTACATCC TGAACCTGCG CTACTTCGAG 
ATGTGCATCC TCATGGTCAT TGCCATGAGT AGCATCGCGC TGGCCGCCGA GGACCCGGTG 
CAGCCCAACG CACCCCGCAA CAACGTGCTG CGATATTTTG ACTATGTTTT CACAGGAGTG 
TTTACCTTTG AGATGGTGAT CAAGATGATC GACCTGGGCC TCGTCCTGCA TCAGGGGGCC 
TATTTCCGTG ACCTGTGGAA CATTCTGGAC TTCATAGTGG TCAGTGGGGC CCTGGTGGCC 
TTTGCCTTCA CGAGCAGTAC ACGTGGCAAT AGCAAAGGAA AGGACATCAA CACCATCAAG 
TCCCTCCGAG TCCTCCGGGT GCTACGACCT CTAAAGACCA TCAAGCGGCT GCCTAAGTTG 
AAGGCCGTAT TTGACTGCGT GGTGAACTCG CTCAAGAACG TCTTCAACAT CCTCATTGTC 
TACATGCTCT TCATGTTCAT CTTCGCCGTG GTGGCCGTGC AGCTCTTCAA GGGCAAATTC 
TTCCACTGCA CGGACGAGTC CAAGGAGTTT GAGAGAGACT GTCGAGGCAA ATACCTCCTT 
TACGAGAAGA ACGAGGTAAA GGCGCGGGAC CGCGAGTGGA AGAAATACGA CTTCCACTAC 
GACAACGTGC TCTGGGCCCT GCTCACGCTC TTTACGGTGT CCACGGGAGA GGGCTGGCCA 
CAGGTCCTCA AGCACTCAGT GGATGCCACT TTTGAGAACC AGGGCCCCAG CCCCGGGTAC 
CGCATGGAAA TGTCCATCTT CTACGTGGTC TACTTTGTGG TGTTTCCCTT CTTCTTTGTC 
AATATCTTTG TGGCCTTGAT CATCATCACC TTCCAGGAGC AGGGAGACAA GATGATGGAA 
GAATACAGCC TAGAGAAAAA TGAGAGGGCC TGCATCGACT TTGCCATCAG TGCCAAGCCG 
CTGACCAGGC ACATGCCCCA GAACAAGCAG AGCTTCCAGT ATCGAATGTG GCAGTTCGTG 
GTGTCCCCAC CCTTTGAGTA CACCATCATG GCCATGATCG CTCTCAACAC CATCGTGCTA 
ATGATGAAGT TCTATGGAGC CTCTGTGGCC TATGAAAACG CCCTTCGAGT GTTCAACATT 
GTCTTCACCT CCCTCTTCTC TCTCGAATGT GTGCTCAAAG TCATGGCTTT TGGGATTCTG 
AATTATTTCC GCGATGCCTG GAACATCTTC GACTTTGTGA CTGTTCTGGG CAGCATCACA 
GACATCCTCG TCACCGAGTT TGGGAATAAC TTCATCAACC TGAGCTTTCT CCGCCTCTTC 
CGTGCTGCCC GACTCATCAA ACTCCTCCGC CAGGGTTACA CCATCCGCAT TCTCCTCTGG 
ACTTTCGTGC AGTCTTTCAA GGCCCTACCT TATGTCTGTC TGCTGATCGC CATGCTCTTC 
TTCATCTATG CCATCATCGG GATGCAGGTG TTTGGCAACA TCGGCATTGA TGGGGAAGAT 
GAGGACAGCG ATGAGGATGA GTTCCAAATC ACGGAGCACA ATAACTTCCG GACCTTCTTC 
CAAGCTCTCA TGCTTCTCTT CCGGAGCGCC ACAGGGGAAG CGTGGCACAA CATCATGCTG 
TCCTGCCTCA GCGGGAAGCC ATGCGACAAG AACTCCGGGA TCCAAAAACC AGAGTGTGGC 
AACGAGTTCG CCTATTTTTA CTTTGTCTCG TTCATCTTCC TTTGCTCATT TCTGATGCTG 
AATCTCTTTG TTGCTGTCAT CATGGACAAC TTCGAGTACC TCACCCGAGA TTCCTCCATC 
CTGGGCCCCC ACCACCTGGA TGAGTACGTG CGTGTCTGGG CAGAGTATGA CCCTGCTGCC 
TGGGGTCGCA TGCCTTACCC GGACATGTAT CAGATGCTGA GACACATGTC CCCGCCCCTG 
GGTCTTGGGA AGAAGTGCCC GGCCAGAGTG GCTTACAAGA GGCTCTTGCG GATGGACCTA 
CCCGTAGCGG ATGACAACAC CGTTCACTTC AACTCCACCT TGATGGCTCT GATCCGAACC 
GCCCTGGATA TCAAAATCGC AAAGGGTGGA GCTGACAAGC AGCAAATGGA CGCAGAGCTC 
CGCAAGGAAA TGATGGCCAT TTGGCCCAAC CTGTCTCAGA AGACCTTGGA TCTGCTGGTC 
ACACCTCACA AGTCCACGGA CCTGACAGTG GGTAAGATCT ACGCAGCCAT GATGATCATG 
GAGTACTACC GGCAGAGCAA GGCCAAGAAG CTGCAGGCCA TGCGAGAGGA GCAGAACCGG 
ACACCACTCA TGTTCCAGCG CATGGAGCCT CCATCGCCAA CACAGGAGGG AGGACCCAGC 
CAAAACGCCC TTCCCTCCAC TCAGCTGGAC CCCGGAGGAG GCCTGATGGC TCAAGAAAGC 
AGCATGAAGG AGAGCCCGTC CTGGGTGACC CAGCGGGCAC AGGAGATGTT CCAGAAGACT 
GGTACCTGGA GCCCAGAGCG AGGGCCACCC ATCGACATGC CTAACAGCCA GCCCAACTCC 
CAGTCTGTGG AGATGAGAGA AATGGGAACT GATGGCTACT CAGACAGCGA ACACTACCTC 
CCCATGGAAG GACAGACCAG GGCCGCCTCC ATGCCCCGCC TCCCAGCAGA GAACCAGAGG 
AGAAGGGGCC GGCCACGTGG AAATAACCTC AGTACCATCT CTGATACCAG CCCCATGAAG 
CGCTCAGCCT CCGTGCTGGG ACCCAAAGCC CGGCGACTGG ATGACTACTC ACTAGAGCGG 
GTACCACCTG AGGAGAACCA AAGGTACCAC CAACGCCGCC GGGACCGTGG CCACCGCACC 
TCTGAGCGCT CTCTGGGCCG ATACACTGAT GTGGACACAG GCCTGGGGAC AGATCTGAGC 
ATGACCACCC AATCGGGTGA CCTGCCCTCC AAAGATCGGG ACCAGGACCG GGGCCGGCCC 
AAGGACCGGA AGCATCGGCC ACACCACCAC CACCACCATC ATCACCATCA TCCCCCGGCC 
CCGGACCGGG AGCGCTACGC ACAGGAGCGG CCGGACACCG GCCGGGCGCG GGCCCGGGAG 
CAGCGCTGGT CCCGCTCGCC CAGCGAGGGT CGGGAGCACG CGACACACAG ACAGGGCAGT 
AGTTCCGTCA GTGGAAGCCC AGCCCCCTCT ACGTCCGGCA CCAGCACGCC GCGGCGCGGC 
CGCCGCCAGC TCCCCCAGAC CCCCTGCACC CCGCGGCCGC TTGTGTCCTA CTCGCCCGCT 
CCGCGCAGGC CTGCGGCGCG CAGGATGGCG GGTCCCCCAG CGCCCCCTGG CGGCTCGCCG 
CGGGGTTGCC GCCGTGCGCC GCGCTGGCCT GCGCATGCGC CTGAGGGCCC GCGGCCGCGC 
GGTGCCGACT ACACCGAGCC CGACAGCCCG CGCGAGCCGC CAGGGGGCGC GCATGAGCCC 
GCGCCGCGGT CGCCCAGGAC TCCGCGCGCC GCGGGCTGCG CGTCACCGCG CCACGGCCGC 
AGGCTGCCCA ATGGTTACTA CGCGGGGCAC GGCGCGCCAC GGCCGCGCAC AGCCCGCAGG 
GGCGCGCACG ACGCGTACAG CGAGAGCGAG GACGACTGGT GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017456669.1
CDS	106..7530
Translation	MVSSPGISERGPWMPRASWVSDPMRSPLNHELLHSSRYPGTDCTLAGSSRTLHVPWSEQMSGSLEGPTLKRRGGVVDHRDVILAHQAHKMHSTPQARRKEWEMARFGDEMPGRYGAGGGGSGPAAGVVVGAAGGRGAGGSRQGGQPGAQRMYKQSMAQRARTMALYNPIPVRQNCLTVNRSLFLFSEDNVVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDDDKTPMSERLDDTEPYFIGIFCFEAGIKIVALGFAFHKGSYLRNGWNVMDFVVVLTGILATVGTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILIFAIIGLEFYMGKFHTTCFEEGTDDIQGESPAPCGTEEPARTCPNGTKCQPYWEGPNNGITQFDNILFAVLTVFQCITMEGWTDLLYNSNDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYMEWISKAEEVILAEDETDVEQRHPFDGALRRATLKKSKTDLLNPEEAEDQLADIASVGSPFARASIKSAKLENSTFFHKKERRMRFYIRRMVKTQAFYWTVLSLVALNTLCVAIVHYNQPEWLSDFLYYAEFIFLGLFMSEMFIKMYGLGTRPYFHSSFNCFDCGVIIGSIFEVIWAVIKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFDEGTPPTNFDTFPAAIMTVFQILTGEDWNEVMYDGIKSQGGVQGGMVFSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAANQKLALQKAKEVAEVSPLSAANMSIAVKEQQKNQKPAKSVWEQRTSEMRKQNLLASREALYGDAAERWPTTYARPLRPDVKTHLDRPLVVDPQENRNNNTNKSRAPEALRQTARPRESARDPDARRAWPSSPERAPGREGPYGRESEPQQREHAPPREHVPWDADPERAKAGDAPRRHTHRPVAEGEPRRHRARRRPGDEPDDRPERRPRPRDATRPARAADGEGDDGERKRRHRHGPPAHDDRERRHRRRKESQGSGVPMSGPNLSTTRPIQQDLGRQDLPLAEDLDNMKNNKLATGEPASPHDSLGHSGLPPSPAKIGNSTNPGPALATNPQNAASRRTPNNPGNPSNPGPPKTPENSLIVTNPSSTQPNSAKTARKPEHMAVEIPPACPPLNHTVVQVNKNANPDPLPKKEEEKKEEEEADPGEDGPKPMPPYSSMFILSTTNPLRRLCHYILNLRYFEMCILMVIAMSSIALAAEDPVQPNAPRNNVLRYFDYVFTGVFTFEMVIKMIDLGLVLHQGAYFRDLWNILDFIVVSGALVAFAFTSSTRGNSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYMLFMFIFAVVAVQLFKGKFFHCTDESKEFERDCRGKYLLYEKNEVKARDREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATFENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEEYSLEKNERACIDFAISAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYTIMAMIALNTIVLMMKFYGASVAYENALRVFNIVFTSLFSLECVLKVMAFGILNYFRDAWNIFDFVTVLGSITDILVTEFGNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIGIDGEDEDSDEDEFQITEHNNFRTFFQALMLLFRSATGEAWHNIMLSCLSGKPCDKNSGIQKPECGNEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRVWAEYDPAAWGRMPYPDMYQMLRHMSPPLGLGKKCPARVAYKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKQQMDAELRKEMMAIWPNLSQKTLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKKLQAMREEQNRTPLMFQRMEPPSPTQEGGPSQNALPSTQLDPGGGLMAQESSMKESPSWVTQRAQEMFQKTGTWSPERGPPIDMPNSQPNSQSVEMREMGTDGYSDSEHYLPMEGQTRAASMPRLPAENQRRRGRPRGNNLSTISDTSPMKRSASVLGPKARRLDDYSLERVPPEENQRYHQRRRDRGHRTSERSLGRYTDVDTGLGTDLSMTTQSGDLPSKDRDQDRGRPKDRKHRPHHHHHHHHHHPPAPDRERYAQERPDTGRARAREQRWSRSPSEGREHATHRQGSSSVSGSPAPSTSGTSTPRRGRRQLPQTPCTPRPLVSYSPAPRRPAARRMAGPPAPPGGSPRGCRRAPRWPAHAPEGPRPRGADYTEPDSPREPPGGAHEPAPRSPRTPRAAGCASPRHGRRLPNGYYAGHGAPRPRTARRGAHDAYSESEDDWC

Target ORF information:

RefSeq Version	XM_017601180.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel subunit alpha1 A (Cacna1a), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017601180.1

ORF Insert Sequence:

ATGGTTTCCT CTCCTGGGAT TTCTGAGCGA GGCCCATGGA TGCCGAGGGC CTCCTGGGTC 
TCTGACCCCA TGCGTTCTCC CCTCAATCAT GAGCTCCTGC ACAGCTCGAG GTACCCAGGC 
ACGGACTGCA CCCTGGCAGG TTCTTCCAGG ACTTTGCATG TCCCGTGGTC CGAGCAGATG 
AGCGGAAGCC TCGAGGGGCC AACTCTGAAA CGGCGTGGTG GCGTGGTGGA CCACCGGGAT 
GTCATCTTGG CCCACCAGGC GCACAAAATG CACAGCACCC CTCAGGCCAG GAGGAAAGAG 
TGGGAAATGG CCCGCTTTGG AGACGAGATG CCGGGCCGCT ACGGCGCAGG CGGAGGAGGC 
TCAGGGCCGG CCGCCGGGGT GGTCGTGGGC GCCGCGGGCG GCCGAGGAGC CGGGGGCAGC 
CGGCAGGGCG GGCAGCCCGG AGCGCAGAGG ATGTACAAGC AGTCGATGGC GCAGAGAGCG 
CGGACCATGG CCCTCTACAA CCCCATCCCT GTCCGCCAGA ACTGCCTCAC GGTCAACCGC 
TCCCTGTTCC TCTTCAGTGA AGACAACGTG GTGAGAAAAT ACGCCAAAAA GATCACGGAA 
TGGCCTCCCT TCGAGTACAT GATCCTGGCC ACCATCATTG CTAACTGCAT CGTCCTGGCC 
CTGGAGCAGC ACCTCCCTGA TGATGACAAG ACACCCATGT CCGAGCGGCT GGATGACACA 
GAACCCTATT TCATTGGCAT CTTCTGTTTT GAGGCTGGAA TTAAGATCGT GGCTCTTGGC 
TTTGCCTTCC ACAAAGGCTC CTACCTGAGG AATGGCTGGA ACGTCATGGA CTTTGTCGTG 
GTGCTAACAG GCATCTTGGC CACTGTCGGG ACGGAGTTTG ATCTACGGAC ACTGAGGGCG 
GTTCGTGTGC TGCGGCCACT CAAGCTGGTG TCTGGAATCC CAAGTTTACA AGTCGTCCTG 
AAGTCAATCA TGAAGGCGAT GATCCCTCTG CTGCAGATCG GCCTCCTCCT GTTTTTTGCA 
ATCCTTATTT TTGCAATCAT AGGGTTAGAA TTTTATATGG GAAAATTTCA TACCACCTGC 
TTTGAAGAGG GGACAGACGA CATCCAGGGT GAGTCGCCAG CTCCGTGTGG GACAGAGGAG 
CCTGCCCGCA CCTGCCCCAA CGGGACCAAA TGTCAGCCGT ACTGGGAAGG GCCCAACAAC 
GGCATCACTC AGTTCGACAA CATCCTGTTT GCTGTGCTCA CTGTTTTCCA GTGCATCACC 
ATGGAAGGCT GGACTGATCT CCTCTACAAT AGCAACGATG CCTCAGGGAA CACTTGGAAC 
TGGTTGTACT TCATCCCCCT CATCATCATC GGCTCCTTTT TTATGCTGAA CCTTGTGCTG 
GGTGTGCTGT CTGGGGAGTT TGCCAAAGAA AGGGAACGTG TAGAGAACCG AAGGGCTTTT 
CTGAAGCTCA GAAGACAACA GCAGATTGAA CGTGAGCTCA ATGGATACAT GGAGTGGATC 
TCGAAAGCAG AAGAGGTGAT CCTCGCGGAG GACGAGACAG ACGTGGAGCA GAGGCACCCT 
TTTGATGGAG CTCTTCGGAG AGCTACTCTG AAGAAAAGCA AGACGGACCT GCTCAACCCT 
GAGGAGGCGG AGGACCAGCT TGCTGACATC GCGTCTGTGG GGTCTCCCTT CGCCAGAGCC 
AGCATCAAAA GTGCCAAGCT GGAGAATTCG ACTTTTTTCC ACAAAAAGGA GAGAAGAATG 
CGTTTCTACA TCCGCCGCAT GGTCAAAACT CAGGCCTTCT ACTGGACCGT GCTCAGTCTG 
GTAGCCCTCA ACACGCTGTG CGTCGCCATT GTCCACTACA ACCAGCCCGA GTGGCTCTCC 
GACTTCCTCT ACTATGCAGA ATTCATTTTC TTAGGACTCT TTATGTCCGA AATGTTTATA 
AAAATGTATG GGCTCGGGAC ACGGCCTTAC TTCCACTCTT CCTTCAACTG CTTTGACTGT 
GGGGTCATCA TCGGGAGCAT CTTTGAAGTC ATCTGGGCCG TCATCAAACC GGGTACATCC 
TTTGGAATCA GCGTGTTACG AGCTCTCAGG TTACTGCGTA TTTTCAAAGT CACAAAGTAC 
TGGGCATCTC TCAGAAACCT GGTTGTCTCC CTCCTCAACT CCATGAAATC CATCATAAGT 
CTGCTGTTCC TCCTCTTCCT CTTCATTGTC GTCTTTGCCC TCTTGGGGAT GCAGCTGTTT 
GGTGGCCAGT TTAATTTTGA CGAGGGGACT CCTCCCACCA ACTTCGACAC TTTTCCAGCA 
GCAATAATGA CTGTGTTTCA GATCCTGACT GGCGAGGATT GGAATGAGGT CATGTATGAT 
GGGATCAAGT CTCAGGGGGG CGTGCAGGGC GGCATGGTGT TCTCCATCTA CTTCATCGTC 
CTCACCCTCT TCGGGAACTA CACCCTGCTG AACGTGTTCT TGGCTATCGC GGTGGACAAC 
CTGGCCAACG CCCAGGAACT CACCAAGGAT GAACAAGAAG AGGAAGAGGC AGCCAATCAG 
AAACTGGCTC TACAGAAAGC CAAGGAGGTG GCAGAAGTGA GTCCCCTGTC TGCAGCCAAC 
ATGTCCATAG CTGTGAAGGA ACAGCAGAAG AACCAGAAGC CTGCCAAGTC GGTGTGGGAG 
CAGCGCACCA GCGAGATGCG CAAGCAGAAC CTGCTGGCTA GCCGCGAGGC GCTGTACGGG 
GACGCGGCTG AGCGCTGGCC CACCACTTAC GCGCGCCCGC TGCGGCCGGA CGTGAAGACG 
CACTTGGACC GGCCGCTCGT GGTGGACCCG CAGGAGAACC GTAACAACAA CACCAACAAG 
AGCCGTGCGC CAGAAGCGCT GCGCCAAACC GCGCGGCCCC GCGAGAGCGC GCGCGACCCC 
GACGCGCGGC GCGCCTGGCC CAGCAGCCCT GAGCGCGCCC CTGGACGAGA GGGCCCGTAT 
GGCCGCGAGA GCGAGCCGCA ACAGCGCGAG CACGCGCCAC CCCGCGAGCA CGTACCCTGG 
GACGCGGATC CTGAGCGCGC CAAGGCCGGG GACGCGCCCC GCCGCCACAC GCACCGGCCT 
GTGGCCGAGG GCGAGCCTCG TCGCCACCGC GCGCGCCGCC GGCCCGGGGA CGAACCGGAC 
GACAGACCGG AGCGCAGGCC GCGTCCCCGC GACGCCACTA GGCCGGCCCG CGCTGCAGAC 
GGCGAAGGCG ATGATGGGGA GCGCAAGCGG CGACACCGAC ACGGGCCGCC GGCCCACGAT 
GACAGGGAGC GCAGACACCG GCGCAGAAAA GAGAGCCAGG GCTCTGGGGT CCCCATGTCT 
GGTCCCAACC TGTCCACCAC CAGGCCAATC CAGCAGGATC TGGGCCGCCA GGACCTGCCA 
CTGGCTGAGG ACCTGGACAA CATGAAGAAC AACAAGTTGG CCACCGGGGA GCCTGCCAGT 
CCCCACGACA GCCTGGGCCA CAGTGGCCTT CCCCCTAGCC CTGCCAAGAT CGGGAACAGC 
ACCAACCCTG GTCCCGCCTT GGCCACCAAT CCCCAGAATG CTGCCAGCCG CAGGACGCCC 
AACAACCCGG GCAACCCGTC CAACCCCGGC CCCCCCAAGA CTCCCGAGAA CAGCCTTATC 
GTCACCAACC CCAGCAGCAC CCAGCCCAAC TCAGCAAAGA CTGCCAGGAA ACCCGAGCAC 
ATGGCGGTGG AGATCCCCCC GGCCTGCCCG CCCCTCAACC ACACTGTGGT CCAAGTAAAC 
AAAAACGCCA ACCCAGACCC ACTGCCAAAG AAAGAGGAAG AGAAGAAGGA GGAAGAGGAG 
GCAGACCCCG GGGAGGATGG CCCAAAGCCC ATGCCGCCCT ACAGCTCCAT GTTCATCCTC 
TCCACCACCA ACCCCCTTCG CCGGCTGTGC CATTACATCC TGAACCTGCG CTACTTCGAG 
ATGTGCATCC TCATGGTCAT TGCCATGAGT AGCATCGCGC TGGCCGCCGA GGACCCGGTG 
CAGCCCAACG CACCCCGCAA CAACGTGCTG CGATATTTTG ACTATGTTTT CACAGGAGTG 
TTTACCTTTG AGATGGTGAT CAAGATGATC GACCTGGGCC TCGTCCTGCA TCAGGGGGCC 
TATTTCCGTG ACCTGTGGAA CATTCTGGAC TTCATAGTGG TCAGTGGGGC CCTGGTGGCC 
TTTGCCTTCA CGAGCAGTAC ACGTGGCAAT AGCAAAGGAA AGGACATCAA CACCATCAAG 
TCCCTCCGAG TCCTCCGGGT GCTACGACCT CTAAAGACCA TCAAGCGGCT GCCTAAGTTG 
AAGGCCGTAT TTGACTGCGT GGTGAACTCG CTCAAGAACG TCTTCAACAT CCTCATTGTC 
TACATGCTCT TCATGTTCAT CTTCGCCGTG GTGGCCGTGC AGCTCTTCAA GGGCAAATTC 
TTCCACTGCA CGGACGAGTC CAAGGAGTTT GAGAGAGACT GTCGAGGCAA ATACCTCCTT 
TACGAGAAGA ACGAGGTAAA GGCGCGGGAC CGCGAGTGGA AGAAATACGA CTTCCACTAC 
GACAACGTGC TCTGGGCCCT GCTCACGCTC TTTACGGTGT CCACGGGAGA GGGCTGGCCA 
CAGGTCCTCA AGCACTCAGT GGATGCCACT TTTGAGAACC AGGGCCCCAG CCCCGGGTAC 
CGCATGGAAA TGTCCATCTT CTACGTGGTC TACTTTGTGG TGTTTCCCTT CTTCTTTGTC 
AATATCTTTG TGGCCTTGAT CATCATCACC TTCCAGGAGC AGGGAGACAA GATGATGGAA 
GAATACAGCC TAGAGAAAAA TGAGAGGGCC TGCATCGACT TTGCCATCAG TGCCAAGCCG 
CTGACCAGGC ACATGCCCCA GAACAAGCAG AGCTTCCAGT ATCGAATGTG GCAGTTCGTG 
GTGTCCCCAC CCTTTGAGTA CACCATCATG GCCATGATCG CTCTCAACAC CATCGTGCTA 
ATGATGAAGT TCTATGGAGC CTCTGTGGCC TATGAAAACG CCCTTCGAGT GTTCAACATT 
GTCTTCACCT CCCTCTTCTC TCTCGAATGT GTGCTCAAAG TCATGGCTTT TGGGATTCTG 
AATTATTTCC GCGATGCCTG GAACATCTTC GACTTTGTGA CTGTTCTGGG CAGCATCACA 
GACATCCTCG TCACCGAGTT TGGGAATAAC TTCATCAACC TGAGCTTTCT CCGCCTCTTC 
CGTGCTGCCC GACTCATCAA ACTCCTCCGC CAGGGTTACA CCATCCGCAT TCTCCTCTGG 
ACTTTCGTGC AGTCTTTCAA GGCCCTACCT TATGTCTGTC TGCTGATCGC CATGCTCTTC 
TTCATCTATG CCATCATCGG GATGCAGGTG TTTGGCAACA TCGGCATTGA TGGGGAAGAT 
GAGGACAGCG ATGAGGATGA GTTCCAAATC ACGGAGCACA ATAACTTCCG GACCTTCTTC 
CAAGCTCTCA TGCTTCTCTT CCGGAGCGCC ACAGGGGAAG CGTGGCACAA CATCATGCTG 
TCCTGCCTCA GCGGGAAGCC ATGCGACAAG AACTCCGGGA TCCAAAAACC AGAGTGTGGC 
AACGAGTTCG CCTATTTTTA CTTTGTCTCG TTCATCTTCC TTTGCTCATT TCTGATGCTG 
AATCTCTTTG TTGCTGTCAT CATGGACAAC TTCGAGTACC TCACCCGAGA TTCCTCCATC 
CTGGGCCCCC ACCACCTGGA TGAGTACGTG CGTGTCTGGG CAGAGTATGA CCCTGCTGCC 
TGGGGTCGCA TGCCTTACCC GGACATGTAT CAGATGCTGA GACACATGTC CCCGCCCCTG 
GGTCTTGGGA AGAAGTGCCC GGCCAGAGTG GCTTACAAGA GGCTCTTGCG GATGGACCTA 
CCCGTAGCGG ATGACAACAC CGTTCACTTC AACTCCACCT TGATGGCTCT GATCCGAACC 
GCCCTGGATA TCAAAATCGC AAAGGGTGGA GCTGACAAGC AGCAAATGGA CGCAGAGCTC 
CGCAAGGAAA TGATGGCCAT TTGGCCCAAC CTGTCTCAGA AGACCTTGGA TCTGCTGGTC 
ACACCTCACA AGTCCACGGA CCTGACAGTG GGTAAGATCT ACGCAGCCAT GATGATCATG 
GAGTACTACC GGCAGAGCAA GGCCAAGAAG CTGCAGGCCA TGCGAGAGGA GCAGAACCGG 
ACACCACTCA TGTTCCAGCG CATGGAGCCT CCATCGCCAA CACAGGAGGG AGGACCCAGC 
CAAAACGCCC TTCCCTCCAC TCAGCTGGAC CCCGGAGGAG GCCTGATGGC TCAAGAAAGC 
AGCATGAAGG AGAGCCCGTC CTGGGTGACC CAGCGGGCAC AGGAGATGTT CCAGAAGACT 
GGTACCTGGA GCCCAGAGCG AGGGCCACCC ATCGACATGC CTAACAGCCA GCCCAACTCC 
CAGTCTGTGG AGATGAGAGA AATGGGAACT GATGGCTACT CAGACAGCGA ACACTACCTC 
CCCATGGAAG GACAGACCAG GGCCGCCTCC ATGCCCCGCC TCCCAGCAGA GAACCAGAGG 
AGAAGGGGCC GGCCACGTGG AAATAACCTC AGTACCATCT CTGATACCAG CCCCATGAAG 
CGCTCAGCCT CCGTGCTGGG ACCCAAAGCC CGGCGACTGG ATGACTACTC ACTAGAGCGG 
GTACCACCTG AGGAGAACCA AAGGTACCAC CAACGCCGCC GGGACCGTGG CCACCGCACC 
TCTGAGCGCT CTCTGGGCCG ATACACTGAT GTGGACACAG GCCTGGGGAC AGATCTGAGC 
ATGACCACCC AATCGGGTGA CCTGCCCTCC AAAGATCGGG ACCAGGACCG GGGCCGGCCC 
AAGGACCGGA AGCATCGGCC ACACCACCAC CACCACCATC ATCACCATCA TCCCCCGGCC 
CCGGACCGGG AGCGCTACGC ACAGGAGCGG CCGGACACCG GCCGGGCGCG GGCCCGGGAG 
CAGCGCTGGT CCCGCTCGCC CAGCGAGGGT CGGGAGCACG CGACACACAG ACAGGGCAGT 
AGTTCCGTCA GTGGAAGCCC AGCCCCCTCT ACGTCCGGCA CCAGCACGCC GCGGCGCGGC 
CGCCGCCAGC TCCCCCAGAC CCCCTGCACC CCGCGGCCGC TTGTGTCCTA CTCGCCCGCT 
CCGCGCAGGC CTGCGGCGCG CAGGATGGCG GGTCCCCCAG CGCCCCCTGG CGGCTCGCCG 
CGGGGTTGCC GCCGTGCGCC GCGCTGGCCT GCGCATGCGC CTGAGGGCCC GCGGCCGCGC 
GGTGCCGACT ACACCGAGCC CGACAGCCCG CGCGAGCCGC CAGGGGGCGC GCATGAGCCC 
GCGCCGCGGT CGCCCAGGAC TCCGCGCGCC GCGGGCTGCG CGTCACCGCG CCACGGCCGC 
AGGCTGCCCA ATGGTTACTA CGCGGGGCAC GGCGCGCCAC GGCCGCGCAC AGCCCGCAGG 
GGCGCGCACG ACGCGTACAG CGAGAGCGAG GACGACTGGT GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1a ( XM_008772356.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008770578.2 Rattus norvegicus calcium voltage-gated channel subunit alpha1 A (Cacna1a), transcript variant X12, mRNA.

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CloneID	ORa59375
Clone ID Related Accession (Same CDS sequence)	XM_008772356.2
Accession Version	XM_008772356.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008772356.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTCCT CTCCTGGGAT TTCTGAGCGA GGCCCATGGA TGCCGAGGGC CTCCTGGGTC 
TCTGACCCCA TGCGTTCTCC CCTCAATCAT GAGCTCCTGC ACAGCTCGAG GTACCCAGGC 
ACGGACTGCA CCCTGGCAGG TTCTTCCAGG ACTTTGCATG TCCCGTGGTC CGAGCAGATG 
AGCGGAAGCC TCGAGGGGCC AACTCTGAAA CGGCGTGGTG GCGTGGTGGA CCACCGGGAT 
GTCATCTTGG CCCACCAGGC GCACAAAATG CACAGCACCC CTCAGGCCAG GAGGAAAGAG 
TGGGAAATGG CCCGCTTTGG AGACGAGATG CCGGGCCGCT ACGGCGCAGG CGGAGGAGGC 
TCAGGGCCGG CCGCCGGGGT GGTCGTGGGC GCCGCGGGCG GCCGAGGAGC CGGGGGCAGC 
CGGCAGGGCG GGCAGCCCGG AGCGCAGAGG ATGTACAAGC AGTCGATGGC GCAGAGAGCG 
CGGACCATGG CCCTCTACAA CCCCATCCCT GTCCGCCAGA ACTGCCTCAC GGTCAACCGC 
TCCCTGTTCC TCTTCAGTGA AGACAACGTG GTGAGAAAAT ACGCCAAAAA GATCACGGAA 
TGGCCTCCCT TCGAGTACAT GATCCTGGCC ACCATCATTG CTAACTGCAT CGTCCTGGCC 
CTGGAGCAGC ACCTCCCTGA TGATGACAAG ACACCCATGT CCGAGCGGCT GGATGACACA 
GAACCCTATT TCATTGGCAT CTTCTGTTTT GAGGCTGGAA TTAAGATCGT GGCTCTTGGC 
TTTGCCTTCC ACAAAGGCTC CTACCTGAGG AATGGCTGGA ACGTCATGGA CTTTGTCGTG 
GTGCTAACAG GCATCTTGGC CACTGTCGGG ACGGAGTTTG ATCTACGGAC ACTGAGGGCG 
GTTCGTGTGC TGCGGCCACT CAAGCTGGTG TCTGGAATCC CAAGTTTACA AGTCGTCCTG 
AAGTCAATCA TGAAGGCGAT GATCCCTCTG CTGCAGATCG GCCTCCTCCT GTTTTTTGCA 
ATCCTTATTT TTGCAATCAT AGGGTTAGAA TTTTATATGG GAAAATTTCA TACCACCTGC 
TTTGAAGAGG GGACAGACGA CATCCAGGGT GAGTCGCCAG CTCCGTGTGG GACAGAGGAG 
CCTGCCCGCA CCTGCCCCAA CGGGACCAAA TGTCAGCCGT ACTGGGAAGG GCCCAACAAC 
GGCATCACTC AGTTCGACAA CATCCTGTTT GCTGTGCTCA CTGTTTTCCA GTGCATCACC 
ATGGAAGGCT GGACTGATCT CCTCTACAAT AGCAACGATG CCTCAGGGAA CACTTGGAAC 
TGGTTGTACT TCATCCCCCT CATCATCATC GGCTCCTTTT TTATGCTGAA CCTTGTGCTG 
GGTGTGCTGT CTGGGGAGTT TGCCAAAGAA AGGGAACGTG TAGAGAACCG AAGGGCTTTT 
CTGAAGCTCA GAAGACAACA GCAGATTGAA CGTGAGCTCA ATGGATACAT GGAGTGGATC 
TCGAAAGCAG AAGAGGTGAT CCTCGCGGAG GACGAGACAG ACGTGGAGCA GAGGCACCCT 
TTTGATGGAG CTCTTCGGAG AGCTACTCTG AAGAAAAGCA AGACGGACCT GCTCAACCCT 
GAGGAGGCGG AGGACCAGCT TGCTGACATC GCGTCTGTGG GGTCTCCCTT CGCCAGAGCC 
AGCATCAAAA GTGCCAAGCT GGAGAATTCG ACTTTTTTCC ACAAAAAGGA GAGAAGAATG 
CGTTTCTACA TCCGCCGCAT GGTCAAAACT CAGGCCTTCT ACTGGACCGT GCTCAGTCTG 
GTAGCCCTCA ACACGCTGTG CGTCGCCATT GTCCACTACA ACCAGCCCGA GTGGCTCTCC 
GACTTCCTCT ACTATGCAGA ATTCATTTTC TTAGGACTCT TTATGTCCGA AATGTTTATA 
AAAATGTATG GGCTCGGGAC ACGGCCTTAC TTCCACTCTT CCTTCAACTG CTTTGACTGT 
GGGGTCATCA TCGGGAGCAT CTTTGAAGTC ATCTGGGCCG TCATCAAACC GGGTACATCC 
TTTGGAATCA GCGTGTTACG AGCTCTCAGG TTACTGCGTA TTTTCAAAGT CACAAAGTAC 
TGGGCATCTC TCAGAAACCT GGTTGTCTCC CTCCTCAACT CCATGAAATC CATCATAAGT 
CTGCTGTTCC TCCTCTTCCT CTTCATTGTC GTCTTTGCCC TCTTGGGGAT GCAGCTGTTT 
GGTGGCCAGT TTAATTTTGA CGAGGGGACT CCTCCCACCA ACTTCGACAC TTTTCCAGCA 
GCAATAATGA CTGTGTTTCA GATCCTGACT GGCGAGGATT GGAATGAGGT CATGTATGAT 
GGGATCAAGT CTCAGGGGGG CGTGCAGGGC GGCATGGTGT TCTCCATCTA CTTCATCGTC 
CTCACCCTCT TCGGGAACTA CACCCTGCTG AACGTGTTCT TGGCTATCGC GGTGGACAAC 
CTGGCCAACG CCCAGGAACT CACCAAGGAT GAACAAGAAG AGGAAGAGGC AGCCAATCAG 
AAACTGGCTC TACAGAAAGC CAAGGAGGTG GCAGAAGTGA GTCCCCTGTC TGCAGCCAAC 
ATGTCCATAG CTGTGAAGGA ACAGCAGAAG AACCAGAAGC CTGCCAAGTC GGTGTGGGAG 
CAGCGCACCA GCGAGATGCG CAAGCAGAAC CTGCTGGCTA GCCGCGAGGC GCTGTACGGG 
GACGCGGCTG AGCGCTGGCC CACCACTTAC GCGCGCCCGC TGCGGCCGGA CGTGAAGACG 
CACTTGGACC GGCCGCTCGT GGTGGACCCG CAGGAGAACC GTAACAACAA CACCAACAAG 
AGCCGTGCGC CAGAAGCGCT GCGCCAAACC GCGCGGCCCC GCGAGAGCGC GCGCGACCCC 
GACGCGCGGC GCGCCTGGCC CAGCAGCCCT GAGCGCGCCC CTGGACGAGA GGGCCCGTAT 
GGCCGCGAGA GCGAGCCGCA ACAGCGCGAG CACGCGCCAC CCCGCGAGCA CGTACCCTGG 
GACGCGGATC CTGAGCGCGC CAAGGCCGGG GACGCGCCCC GCCGCCACAC GCACCGGCCT 
GTGGCCGAGG GCGAGCCTCG TCGCCACCGC GCGCGCCGCC GGCCCGGGGA CGAACCGGAC 
GACAGACCGG AGCGCAGGCC GCGTCCCCGC GACGCCACTA GGCCGGCCCG CGCTGCAGAC 
GGCGAAGGCG ATGATGGGGA GCGCAAGCGG CGACACCGAC ACGGGCCGCC GGCCCACGAT 
GACAGGGAGC GCAGACACCG GCGCAGAAAA GAGAGCCAGG GCTCTGGGGT CCCCATGTCT 
GGTCCCAACC TGTCCACCAC CAGGCCAATC CAGCAGGATC TGGGCCGCCA GGACCTGCCA 
CTGGCTGAGG ACCTGGACAA CATGAAGAAC AACAAGTTGG CCACCGGGGA GCCTGCCAGT 
CCCCACGACA GCCTGGGCCA CAGTGGCCTT CCCCCTAGCC CTGCCAAGAT CGGGAACAGC 
ACCAACCCTG GTCCCGCCTT GGCCACCAAT CCCCAGAATG CTGCCAGCCG CAGGACGCCC 
AACAACCCGG GCAACCCGTC CAACCCCGGC CCCCCCAAGA CTCCCGAGAA CAGCCTTATC 
GTCACCAACC CCAGCAGCAC CCAGCCCAAC TCAGCAAAGA CTGCCAGGAA ACCCGAGCAC 
ATGGCGGTGG AGATCCCCCC GGCCTGCCCG CCCCTCAACC ACACTGTGGT CCAAGTAAAC 
AAAAACGCCA ACCCAGACCC ACTGCCAAAG AAAGAGGAAG AGAAGAAGGA GGAAGAGGAG 
GCAGACCCCG GGGAGGATGG CCCAAAGCCC ATGCCGCCCT ACAGCTCCAT GTTCATCCTC 
TCCACCACCA ACCCCCTTCG CCGGCTGTGC CATTACATCC TGAACCTGCG CTACTTCGAG 
ATGTGCATCC TCATGGTCAT TGCCATGAGT AGCATCGCGC TGGCCGCCGA GGACCCGGTG 
CAGCCCAACG CACCCCGCAA CAACGTGCTG CGATATTTTG ACTATGTTTT CACAGGAGTG 
TTTACCTTTG AGATGGTGAT CAAGATGATC GACCTGGGCC TCGTCCTGCA TCAGGGGGCC 
TATTTCCGTG ACCTGTGGAA CATTCTGGAC TTCATAGTGG TCAGTGGGGC CCTGGTGGCC 
TTTGCCTTCA CTGGCAATAG CAAAGGAAAG GACATCAACA CCATCAAGTC CCTCCGAGTC 
CTCCGGGTGC TACGACCTCT AAAGACCATC AAGCGGCTGC CTAAGTTGAA GGCCGTATTT 
GACTGCGTGG TGAACTCGCT CAAGAACGTC TTCAACATCC TCATTGTCTA CATGCTCTTC 
ATGTTCATCT TCGCCGTGGT GGCCGTGCAG CTCTTCAAGG GCAAATTCTT CCACTGCACG 
GACGAGTCCA AGGAGTTTGA GAGAGACTGT CGAGGCAAAT ACCTCCTTTA CGAGAAGAAC 
GAGGTAAAGG CGCGGGACCG CGAGTGGAAG AAATACGACT TCCACTACGA CAACGTGCTC 
TGGGCCCTGC TCACGCTCTT TACGGTGTCC ACGGGAGAGG GCTGGCCACA GGTCCTCAAG 
CACTCAGTGG ATGCCACTTT TGAGAACCAG GGCCCCAGCC CCGGGTACCG CATGGAAATG 
TCCATCTTCT ACGTGGTCTA CTTTGTGGTG TTTCCCTTCT TCTTTGTCAA TATCTTTGTG 
GCCTTGATCA TCATCACCTT CCAGGAGCAG GGAGACAAGA TGATGGAAGA ATACAGCCTA 
GAGAAAAATG AGAGGGCCTG CATCGACTTT GCCATCAGTG CCAAGCCGCT GACCAGGCAC 
ATGCCCCAGA ACAAGCAGAG CTTCCAGTAT CGAATGTGGC AGTTCGTGGT GTCCCCACCC 
TTTGAGTACA CCATCATGGC CATGATCGCT CTCAACACCA TCGTGCTAAT GATGAAGTTC 
TATGGAGCCT CTGTGGCCTA TGAAAACGCC CTTCGAGTGT TCAACATTGT CTTCACCTCC 
CTCTTCTCTC TCGAATGTGT GCTCAAAGTC ATGGCTTTTG GGATTCTGAA TTATTTCCGC 
GATGCCTGGA ACATCTTCGA CTTTGTGACT GTTCTGGGCA GCATCACAGA CATCCTCGTC 
ACCGAGTTTG GGAATCCGAA TAACTTCATC AACCTGAGCT TTCTCCGCCT CTTCCGTGCT 
GCCCGACTCA TCAAACTCCT CCGCCAGGGT TACACCATCC GCATTCTCCT CTGGACTTTC 
GTGCAGTCTT TCAAGGCCCT ACCTTATGTC TGTCTGCTGA TCGCCATGCT CTTCTTCATC 
TATGCCATCA TCGGGATGCA GGTGTTTGGC AACATCGGCA TTGATGGGGA AGATGAGGAC 
AGCGATGAGG ATGAGTTCCA AATCACGGAG CACAATAACT TCCGGACCTT CTTCCAAGCT 
CTCATGCTTC TCTTCCGGAG CGCCACAGGG GAAGCGTGGC ACAACATCAT GCTGTCCTGC 
CTCAGCGGGA AGCCATGCGA CAAGAACTCC GGGATCCAAA AACCAGAGTG TGGCAACGAG 
TTCGCCTATT TTTACTTTGT CTCGTTCATC TTCCTTTGCT CATTTCTGAT GCTGAATCTC 
TTTGTTGCTG TCATCATGGA CAACTTCGAG TACCTCACCC GAGATTCCTC CATCCTGGGC 
CCCCACCACC TGGATGAGTA CGTGCGTGTC TGGGCAGAGT ATGACCCTGC TGCCTGGGGT 
CGCATGCCTT ACCCGGACAT GTATCAGATG CTGAGACACA TGTCCCCGCC CCTGGGTCTT 
GGGAAGAAGT GCCCGGCCAG AGTGGCTTAC AAGAGGCTCT TGCGGATGGA CCTACCCGTA 
GCGGATGACA ACACCGTTCA CTTCAACTCC ACCTTGATGG CTCTGATCCG AACCGCCCTG 
GATATCAAAA TCGCAAAGGG TGGAGCTGAC AAGCAGCAAA TGGACGCAGA GCTCCGCAAG 
GAAATGATGG CCATTTGGCC CAACCTGTCT CAGAAGACCT TGGATCTGCT GGTCACACCT 
CACAAGTCCA CGGACCTGAC AGTGGGTAAG ATCTACGCAG CCATGATGAT CATGGAGTAC 
TACCGGCAGA GCAAGGCCAA GAAGCTGCAG GCCATGCGAG AGGAGCAGAA CCGGACACCA 
CTCATGTTCC AGCGCATGGA GCCTCCATCG CCAACACAGG AGGGAGGACC CAGCCAAAAC 
GCCCTTCCCT CCACTCAGCT GGACCCCGGA GGAGGCCTGA TGGCTCAAGA AAGCAGCATG 
AAGGAGAGCC CGTCCTGGGT GACCCAGCGG GCACAGGAGA TGTTCCAGAA GACTGGTACC 
TGGAGCCCAG AGCGAGGGCC ACCCATCGAC ATGCCTAACA GCCAGCCCAA CTCCCAGTCT 
GTGGAGATGA GAGAAATGGG AACTGATGGC TACTCAGACA GCGAACACTA CCTCCCCATG 
GAAGGACAGA CCAGGGCCGC CTCCATGCCC CGCCTCCCAG CAGAGAACCA GAGGAGAAGG 
GGCCGGCCAC GTGGAAATAA CCTCAGTACC ATCTCTGATA CCAGCCCCAT GAAGCGCTCA 
GCCTCCGTGC TGGGACCCAA AGCCCGGCGA CTGGATGACT ACTCACTAGA GCGGGTACCA 
CCTGAGGAGA ACCAAAGGTA CCACCAACGC CGCCGGGACC GTGGCCACCG CACCTCTGAG 
CGCTCTCTGG GCCGATACAC TGATGTGGAC ACAGGCCTGG GGACAGATCT GAGCATGACC 
ACCCAATCGG GTGACCTGCC CTCCAAAGAT CGGGACCAGG ACCGGGGCCG GCCCAAGGAC 
CGGAAGCATC GGCCACACCA CCACCACCAC CATCATCACC ATCATCCCCC GGCCCCGGAC 
CGGGAGCGCT ACGCACAGGA GCGGCCGGAC ACCGGCCGGG CGCGGGCCCG GGAGCAGCGC 
TGGTCCCGCT CGCCCAGCGA GGGTCGGGAG CACGCGACAC ACAGACAGGG CAGTAGTTCC 
GTCAGTGGAA GCCCAGCCCC CTCTACGTCC GGCACCAGCA CGCCGCGGCG CGGCCGCCGC 
CAGCTCCCCC AGACCCCCTG CACCCCGCGG CCGCTTGTGT CCTACTCGCC CGCTCCGCGC 
AGGCCTGCGG CGCGCAGGAT GGCGGGTCCC CCAGCGCCCC CTGGCGGCTC GCCGCGGGGT 
TGCCGCCGTG CGCCGCGCTG GCCTGCGCAT GCGCCTGAGG GCCCGCGGCC GCGCGGTGCC 
GACTACACCG AGCCCGACAG CCCGCGCGAG CCGCCAGGGG GCGCGCATGA GCCCGCGCCG 
CGGTCGCCCA GGACTCCGCG CGCCGCGGGC TGCGCGTCAC CGCGCCACGG CCGCAGGCTG 
CCCAATGGTT ACTACGCGGG GCACGGCGCG CCACGGCCGC GCACAGCCCG CAGGGGCGCG 
CACGACGCGT ACAGCGAGAG CGAGGACGAC TGGTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008770578.2
CDS	105..7523
Translation	MVSSPGISERGPWMPRASWVSDPMRSPLNHELLHSSRYPGTDCTLAGSSRTLHVPWSEQMSGSLEGPTLKRRGGVVDHRDVILAHQAHKMHSTPQARRKEWEMARFGDEMPGRYGAGGGGSGPAAGVVVGAAGGRGAGGSRQGGQPGAQRMYKQSMAQRARTMALYNPIPVRQNCLTVNRSLFLFSEDNVVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDDDKTPMSERLDDTEPYFIGIFCFEAGIKIVALGFAFHKGSYLRNGWNVMDFVVVLTGILATVGTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILIFAIIGLEFYMGKFHTTCFEEGTDDIQGESPAPCGTEEPARTCPNGTKCQPYWEGPNNGITQFDNILFAVLTVFQCITMEGWTDLLYNSNDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYMEWISKAEEVILAEDETDVEQRHPFDGALRRATLKKSKTDLLNPEEAEDQLADIASVGSPFARASIKSAKLENSTFFHKKERRMRFYIRRMVKTQAFYWTVLSLVALNTLCVAIVHYNQPEWLSDFLYYAEFIFLGLFMSEMFIKMYGLGTRPYFHSSFNCFDCGVIIGSIFEVIWAVIKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFDEGTPPTNFDTFPAAIMTVFQILTGEDWNEVMYDGIKSQGGVQGGMVFSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAANQKLALQKAKEVAEVSPLSAANMSIAVKEQQKNQKPAKSVWEQRTSEMRKQNLLASREALYGDAAERWPTTYARPLRPDVKTHLDRPLVVDPQENRNNNTNKSRAPEALRQTARPRESARDPDARRAWPSSPERAPGREGPYGRESEPQQREHAPPREHVPWDADPERAKAGDAPRRHTHRPVAEGEPRRHRARRRPGDEPDDRPERRPRPRDATRPARAADGEGDDGERKRRHRHGPPAHDDRERRHRRRKESQGSGVPMSGPNLSTTRPIQQDLGRQDLPLAEDLDNMKNNKLATGEPASPHDSLGHSGLPPSPAKIGNSTNPGPALATNPQNAASRRTPNNPGNPSNPGPPKTPENSLIVTNPSSTQPNSAKTARKPEHMAVEIPPACPPLNHTVVQVNKNANPDPLPKKEEEKKEEEEADPGEDGPKPMPPYSSMFILSTTNPLRRLCHYILNLRYFEMCILMVIAMSSIALAAEDPVQPNAPRNNVLRYFDYVFTGVFTFEMVIKMIDLGLVLHQGAYFRDLWNILDFIVVSGALVAFAFTGNSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYMLFMFIFAVVAVQLFKGKFFHCTDESKEFERDCRGKYLLYEKNEVKARDREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATFENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEEYSLEKNERACIDFAISAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYTIMAMIALNTIVLMMKFYGASVAYENALRVFNIVFTSLFSLECVLKVMAFGILNYFRDAWNIFDFVTVLGSITDILVTEFGNPNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIGIDGEDEDSDEDEFQITEHNNFRTFFQALMLLFRSATGEAWHNIMLSCLSGKPCDKNSGIQKPECGNEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRVWAEYDPAAWGRMPYPDMYQMLRHMSPPLGLGKKCPARVAYKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKQQMDAELRKEMMAIWPNLSQKTLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKKLQAMREEQNRTPLMFQRMEPPSPTQEGGPSQNALPSTQLDPGGGLMAQESSMKESPSWVTQRAQEMFQKTGTWSPERGPPIDMPNSQPNSQSVEMREMGTDGYSDSEHYLPMEGQTRAASMPRLPAENQRRRGRPRGNNLSTISDTSPMKRSASVLGPKARRLDDYSLERVPPEENQRYHQRRRDRGHRTSERSLGRYTDVDTGLGTDLSMTTQSGDLPSKDRDQDRGRPKDRKHRPHHHHHHHHHHPPAPDRERYAQERPDTGRARAREQRWSRSPSEGREHATHRQGSSSVSGSPAPSTSGTSTPRRGRRQLPQTPCTPRPLVSYSPAPRRPAARRMAGPPAPPGGSPRGCRRAPRWPAHAPEGPRPRGADYTEPDSPREPPGGAHEPAPRSPRTPRAAGCASPRHGRRLPNGYYAGHGAPRPRTARRGAHDAYSESEDDWC

Target ORF information:

RefSeq Version	XM_008772356.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel subunit alpha1 A (Cacna1a), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772356.2

ORF Insert Sequence:

ATGGTTTCCT CTCCTGGGAT TTCTGAGCGA GGCCCATGGA TGCCGAGGGC CTCCTGGGTC 
TCTGACCCCA TGCGTTCTCC CCTCAATCAT GAGCTCCTGC ACAGCTCGAG GTACCCAGGC 
ACGGACTGCA CCCTGGCAGG TTCTTCCAGG ACTTTGCATG TCCCGTGGTC CGAGCAGATG 
AGCGGAAGCC TCGAGGGGCC AACTCTGAAA CGGCGTGGTG GCGTGGTGGA CCACCGGGAT 
GTCATCTTGG CCCACCAGGC GCACAAAATG CACAGCACCC CTCAGGCCAG GAGGAAAGAG 
TGGGAAATGG CCCGCTTTGG AGACGAGATG CCGGGCCGCT ACGGCGCAGG CGGAGGAGGC 
TCAGGGCCGG CCGCCGGGGT GGTCGTGGGC GCCGCGGGCG GCCGAGGAGC CGGGGGCAGC 
CGGCAGGGCG GGCAGCCCGG AGCGCAGAGG ATGTACAAGC AGTCGATGGC GCAGAGAGCG 
CGGACCATGG CCCTCTACAA CCCCATCCCT GTCCGCCAGA ACTGCCTCAC GGTCAACCGC 
TCCCTGTTCC TCTTCAGTGA AGACAACGTG GTGAGAAAAT ACGCCAAAAA GATCACGGAA 
TGGCCTCCCT TCGAGTACAT GATCCTGGCC ACCATCATTG CTAACTGCAT CGTCCTGGCC 
CTGGAGCAGC ACCTCCCTGA TGATGACAAG ACACCCATGT CCGAGCGGCT GGATGACACA 
GAACCCTATT TCATTGGCAT CTTCTGTTTT GAGGCTGGAA TTAAGATCGT GGCTCTTGGC 
TTTGCCTTCC ACAAAGGCTC CTACCTGAGG AATGGCTGGA ACGTCATGGA CTTTGTCGTG 
GTGCTAACAG GCATCTTGGC CACTGTCGGG ACGGAGTTTG ATCTACGGAC ACTGAGGGCG 
GTTCGTGTGC TGCGGCCACT CAAGCTGGTG TCTGGAATCC CAAGTTTACA AGTCGTCCTG 
AAGTCAATCA TGAAGGCGAT GATCCCTCTG CTGCAGATCG GCCTCCTCCT GTTTTTTGCA 
ATCCTTATTT TTGCAATCAT AGGGTTAGAA TTTTATATGG GAAAATTTCA TACCACCTGC 
TTTGAAGAGG GGACAGACGA CATCCAGGGT GAGTCGCCAG CTCCGTGTGG GACAGAGGAG 
CCTGCCCGCA CCTGCCCCAA CGGGACCAAA TGTCAGCCGT ACTGGGAAGG GCCCAACAAC 
GGCATCACTC AGTTCGACAA CATCCTGTTT GCTGTGCTCA CTGTTTTCCA GTGCATCACC 
ATGGAAGGCT GGACTGATCT CCTCTACAAT AGCAACGATG CCTCAGGGAA CACTTGGAAC 
TGGTTGTACT TCATCCCCCT CATCATCATC GGCTCCTTTT TTATGCTGAA CCTTGTGCTG 
GGTGTGCTGT CTGGGGAGTT TGCCAAAGAA AGGGAACGTG TAGAGAACCG AAGGGCTTTT 
CTGAAGCTCA GAAGACAACA GCAGATTGAA CGTGAGCTCA ATGGATACAT GGAGTGGATC 
TCGAAAGCAG AAGAGGTGAT CCTCGCGGAG GACGAGACAG ACGTGGAGCA GAGGCACCCT 
TTTGATGGAG CTCTTCGGAG AGCTACTCTG AAGAAAAGCA AGACGGACCT GCTCAACCCT 
GAGGAGGCGG AGGACCAGCT TGCTGACATC GCGTCTGTGG GGTCTCCCTT CGCCAGAGCC 
AGCATCAAAA GTGCCAAGCT GGAGAATTCG ACTTTTTTCC ACAAAAAGGA GAGAAGAATG 
CGTTTCTACA TCCGCCGCAT GGTCAAAACT CAGGCCTTCT ACTGGACCGT GCTCAGTCTG 
GTAGCCCTCA ACACGCTGTG CGTCGCCATT GTCCACTACA ACCAGCCCGA GTGGCTCTCC 
GACTTCCTCT ACTATGCAGA ATTCATTTTC TTAGGACTCT TTATGTCCGA AATGTTTATA 
AAAATGTATG GGCTCGGGAC ACGGCCTTAC TTCCACTCTT CCTTCAACTG CTTTGACTGT 
GGGGTCATCA TCGGGAGCAT CTTTGAAGTC ATCTGGGCCG TCATCAAACC GGGTACATCC 
TTTGGAATCA GCGTGTTACG AGCTCTCAGG TTACTGCGTA TTTTCAAAGT CACAAAGTAC 
TGGGCATCTC TCAGAAACCT GGTTGTCTCC CTCCTCAACT CCATGAAATC CATCATAAGT 
CTGCTGTTCC TCCTCTTCCT CTTCATTGTC GTCTTTGCCC TCTTGGGGAT GCAGCTGTTT 
GGTGGCCAGT TTAATTTTGA CGAGGGGACT CCTCCCACCA ACTTCGACAC TTTTCCAGCA 
GCAATAATGA CTGTGTTTCA GATCCTGACT GGCGAGGATT GGAATGAGGT CATGTATGAT 
GGGATCAAGT CTCAGGGGGG CGTGCAGGGC GGCATGGTGT TCTCCATCTA CTTCATCGTC 
CTCACCCTCT TCGGGAACTA CACCCTGCTG AACGTGTTCT TGGCTATCGC GGTGGACAAC 
CTGGCCAACG CCCAGGAACT CACCAAGGAT GAACAAGAAG AGGAAGAGGC AGCCAATCAG 
AAACTGGCTC TACAGAAAGC CAAGGAGGTG GCAGAAGTGA GTCCCCTGTC TGCAGCCAAC 
ATGTCCATAG CTGTGAAGGA ACAGCAGAAG AACCAGAAGC CTGCCAAGTC GGTGTGGGAG 
CAGCGCACCA GCGAGATGCG CAAGCAGAAC CTGCTGGCTA GCCGCGAGGC GCTGTACGGG 
GACGCGGCTG AGCGCTGGCC CACCACTTAC GCGCGCCCGC TGCGGCCGGA CGTGAAGACG 
CACTTGGACC GGCCGCTCGT GGTGGACCCG CAGGAGAACC GTAACAACAA CACCAACAAG 
AGCCGTGCGC CAGAAGCGCT GCGCCAAACC GCGCGGCCCC GCGAGAGCGC GCGCGACCCC 
GACGCGCGGC GCGCCTGGCC CAGCAGCCCT GAGCGCGCCC CTGGACGAGA GGGCCCGTAT 
GGCCGCGAGA GCGAGCCGCA ACAGCGCGAG CACGCGCCAC CCCGCGAGCA CGTACCCTGG 
GACGCGGATC CTGAGCGCGC CAAGGCCGGG GACGCGCCCC GCCGCCACAC GCACCGGCCT 
GTGGCCGAGG GCGAGCCTCG TCGCCACCGC GCGCGCCGCC GGCCCGGGGA CGAACCGGAC 
GACAGACCGG AGCGCAGGCC GCGTCCCCGC GACGCCACTA GGCCGGCCCG CGCTGCAGAC 
GGCGAAGGCG ATGATGGGGA GCGCAAGCGG CGACACCGAC ACGGGCCGCC GGCCCACGAT 
GACAGGGAGC GCAGACACCG GCGCAGAAAA GAGAGCCAGG GCTCTGGGGT CCCCATGTCT 
GGTCCCAACC TGTCCACCAC CAGGCCAATC CAGCAGGATC TGGGCCGCCA GGACCTGCCA 
CTGGCTGAGG ACCTGGACAA CATGAAGAAC AACAAGTTGG CCACCGGGGA GCCTGCCAGT 
CCCCACGACA GCCTGGGCCA CAGTGGCCTT CCCCCTAGCC CTGCCAAGAT CGGGAACAGC 
ACCAACCCTG GTCCCGCCTT GGCCACCAAT CCCCAGAATG CTGCCAGCCG CAGGACGCCC 
AACAACCCGG GCAACCCGTC CAACCCCGGC CCCCCCAAGA CTCCCGAGAA CAGCCTTATC 
GTCACCAACC CCAGCAGCAC CCAGCCCAAC TCAGCAAAGA CTGCCAGGAA ACCCGAGCAC 
ATGGCGGTGG AGATCCCCCC GGCCTGCCCG CCCCTCAACC ACACTGTGGT CCAAGTAAAC 
AAAAACGCCA ACCCAGACCC ACTGCCAAAG AAAGAGGAAG AGAAGAAGGA GGAAGAGGAG 
GCAGACCCCG GGGAGGATGG CCCAAAGCCC ATGCCGCCCT ACAGCTCCAT GTTCATCCTC 
TCCACCACCA ACCCCCTTCG CCGGCTGTGC CATTACATCC TGAACCTGCG CTACTTCGAG 
ATGTGCATCC TCATGGTCAT TGCCATGAGT AGCATCGCGC TGGCCGCCGA GGACCCGGTG 
CAGCCCAACG CACCCCGCAA CAACGTGCTG CGATATTTTG ACTATGTTTT CACAGGAGTG 
TTTACCTTTG AGATGGTGAT CAAGATGATC GACCTGGGCC TCGTCCTGCA TCAGGGGGCC 
TATTTCCGTG ACCTGTGGAA CATTCTGGAC TTCATAGTGG TCAGTGGGGC CCTGGTGGCC 
TTTGCCTTCA CTGGCAATAG CAAAGGAAAG GACATCAACA CCATCAAGTC CCTCCGAGTC 
CTCCGGGTGC TACGACCTCT AAAGACCATC AAGCGGCTGC CTAAGTTGAA GGCCGTATTT 
GACTGCGTGG TGAACTCGCT CAAGAACGTC TTCAACATCC TCATTGTCTA CATGCTCTTC 
ATGTTCATCT TCGCCGTGGT GGCCGTGCAG CTCTTCAAGG GCAAATTCTT CCACTGCACG 
GACGAGTCCA AGGAGTTTGA GAGAGACTGT CGAGGCAAAT ACCTCCTTTA CGAGAAGAAC 
GAGGTAAAGG CGCGGGACCG CGAGTGGAAG AAATACGACT TCCACTACGA CAACGTGCTC 
TGGGCCCTGC TCACGCTCTT TACGGTGTCC ACGGGAGAGG GCTGGCCACA GGTCCTCAAG 
CACTCAGTGG ATGCCACTTT TGAGAACCAG GGCCCCAGCC CCGGGTACCG CATGGAAATG 
TCCATCTTCT ACGTGGTCTA CTTTGTGGTG TTTCCCTTCT TCTTTGTCAA TATCTTTGTG 
GCCTTGATCA TCATCACCTT CCAGGAGCAG GGAGACAAGA TGATGGAAGA ATACAGCCTA 
GAGAAAAATG AGAGGGCCTG CATCGACTTT GCCATCAGTG CCAAGCCGCT GACCAGGCAC 
ATGCCCCAGA ACAAGCAGAG CTTCCAGTAT CGAATGTGGC AGTTCGTGGT GTCCCCACCC 
TTTGAGTACA CCATCATGGC CATGATCGCT CTCAACACCA TCGTGCTAAT GATGAAGTTC 
TATGGAGCCT CTGTGGCCTA TGAAAACGCC CTTCGAGTGT TCAACATTGT CTTCACCTCC 
CTCTTCTCTC TCGAATGTGT GCTCAAAGTC ATGGCTTTTG GGATTCTGAA TTATTTCCGC 
GATGCCTGGA ACATCTTCGA CTTTGTGACT GTTCTGGGCA GCATCACAGA CATCCTCGTC 
ACCGAGTTTG GGAATCCGAA TAACTTCATC AACCTGAGCT TTCTCCGCCT CTTCCGTGCT 
GCCCGACTCA TCAAACTCCT CCGCCAGGGT TACACCATCC GCATTCTCCT CTGGACTTTC 
GTGCAGTCTT TCAAGGCCCT ACCTTATGTC TGTCTGCTGA TCGCCATGCT CTTCTTCATC 
TATGCCATCA TCGGGATGCA GGTGTTTGGC AACATCGGCA TTGATGGGGA AGATGAGGAC 
AGCGATGAGG ATGAGTTCCA AATCACGGAG CACAATAACT TCCGGACCTT CTTCCAAGCT 
CTCATGCTTC TCTTCCGGAG CGCCACAGGG GAAGCGTGGC ACAACATCAT GCTGTCCTGC 
CTCAGCGGGA AGCCATGCGA CAAGAACTCC GGGATCCAAA AACCAGAGTG TGGCAACGAG 
TTCGCCTATT TTTACTTTGT CTCGTTCATC TTCCTTTGCT CATTTCTGAT GCTGAATCTC 
TTTGTTGCTG TCATCATGGA CAACTTCGAG TACCTCACCC GAGATTCCTC CATCCTGGGC 
CCCCACCACC TGGATGAGTA CGTGCGTGTC TGGGCAGAGT ATGACCCTGC TGCCTGGGGT 
CGCATGCCTT ACCCGGACAT GTATCAGATG CTGAGACACA TGTCCCCGCC CCTGGGTCTT 
GGGAAGAAGT GCCCGGCCAG AGTGGCTTAC AAGAGGCTCT TGCGGATGGA CCTACCCGTA 
GCGGATGACA ACACCGTTCA CTTCAACTCC ACCTTGATGG CTCTGATCCG AACCGCCCTG 
GATATCAAAA TCGCAAAGGG TGGAGCTGAC AAGCAGCAAA TGGACGCAGA GCTCCGCAAG 
GAAATGATGG CCATTTGGCC CAACCTGTCT CAGAAGACCT TGGATCTGCT GGTCACACCT 
CACAAGTCCA CGGACCTGAC AGTGGGTAAG ATCTACGCAG CCATGATGAT CATGGAGTAC 
TACCGGCAGA GCAAGGCCAA GAAGCTGCAG GCCATGCGAG AGGAGCAGAA CCGGACACCA 
CTCATGTTCC AGCGCATGGA GCCTCCATCG CCAACACAGG AGGGAGGACC CAGCCAAAAC 
GCCCTTCCCT CCACTCAGCT GGACCCCGGA GGAGGCCTGA TGGCTCAAGA AAGCAGCATG 
AAGGAGAGCC CGTCCTGGGT GACCCAGCGG GCACAGGAGA TGTTCCAGAA GACTGGTACC 
TGGAGCCCAG AGCGAGGGCC ACCCATCGAC ATGCCTAACA GCCAGCCCAA CTCCCAGTCT 
GTGGAGATGA GAGAAATGGG AACTGATGGC TACTCAGACA GCGAACACTA CCTCCCCATG 
GAAGGACAGA CCAGGGCCGC CTCCATGCCC CGCCTCCCAG CAGAGAACCA GAGGAGAAGG 
GGCCGGCCAC GTGGAAATAA CCTCAGTACC ATCTCTGATA CCAGCCCCAT GAAGCGCTCA 
GCCTCCGTGC TGGGACCCAA AGCCCGGCGA CTGGATGACT ACTCACTAGA GCGGGTACCA 
CCTGAGGAGA ACCAAAGGTA CCACCAACGC CGCCGGGACC GTGGCCACCG CACCTCTGAG 
CGCTCTCTGG GCCGATACAC TGATGTGGAC ACAGGCCTGG GGACAGATCT GAGCATGACC 
ACCCAATCGG GTGACCTGCC CTCCAAAGAT CGGGACCAGG ACCGGGGCCG GCCCAAGGAC 
CGGAAGCATC GGCCACACCA CCACCACCAC CATCATCACC ATCATCCCCC GGCCCCGGAC 
CGGGAGCGCT ACGCACAGGA GCGGCCGGAC ACCGGCCGGG CGCGGGCCCG GGAGCAGCGC 
TGGTCCCGCT CGCCCAGCGA GGGTCGGGAG CACGCGACAC ACAGACAGGG CAGTAGTTCC 
GTCAGTGGAA GCCCAGCCCC CTCTACGTCC GGCACCAGCA CGCCGCGGCG CGGCCGCCGC 
CAGCTCCCCC AGACCCCCTG CACCCCGCGG CCGCTTGTGT CCTACTCGCC CGCTCCGCGC 
AGGCCTGCGG CGCGCAGGAT GGCGGGTCCC CCAGCGCCCC CTGGCGGCTC GCCGCGGGGT 
TGCCGCCGTG CGCCGCGCTG GCCTGCGCAT GCGCCTGAGG GCCCGCGGCC GCGCGGTGCC 
GACTACACCG AGCCCGACAG CCCGCGCGAG CCGCCAGGGG GCGCGCATGA GCCCGCGCCG 
CGGTCGCCCA GGACTCCGCG CGCCGCGGGC TGCGCGTCAC CGCGCCACGG CCGCAGGCTG 
CCCAATGGTT ACTACGCGGG GCACGGCGCG CCACGGCCGC GCACAGCCCG CAGGGGCGCG 
CACGACGCGT ACAGCGAGAG CGAGGACGAC TGGTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1a ( XM_017601182.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017456671.1 Rattus norvegicus calcium voltage-gated channel subunit alpha1 A (Cacna1a), transcript variant X13, mRNA.

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CloneID	ORa59376
Clone ID Related Accession (Same CDS sequence)	XM_017601182.1
Accession Version	XM_017601182.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7413bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTCCT CTCCTGGGAT TTCTGAGCGA GGCCCATGGA TGCCGAGGGC CTCCTGGGTC 
TCTGACCCCA TGCGTTCTCC CCTCAATCAT GAGCTCCTGC ACAGCTCGAG GTACCCAGGC 
ACGGACTGCA CCCTGGCAGG TTCTTCCAGG ACTTTGCATG TCCCGTGGTC CGAGCAGATG 
AGCGGAAGCC TCGAGGGGCC AACTCTGAAA CGGCGTGGTG GCGTGGTGGA CCACCGGGAT 
GTCATCTTGG CCCACCAGGC GCACAAAATG CACAGCACCC CTCAGGCCAG GAGGAAAGAG 
TGGGAAATGG CCCGCTTTGG AGACGAGATG CCGGGCCGCT ACGGCGCAGG CGGAGGAGGC 
TCAGGGCCGG CCGCCGGGGT GGTCGTGGGC GCCGCGGGCG GCCGAGGAGC CGGGGGCAGC 
CGGCAGGGCG GGCAGCCCGG AGCGCAGAGG ATGTACAAGC AGTCGATGGC GCAGAGAGCG 
CGGACCATGG CCCTCTACAA CCCCATCCCT GTCCGCCAGA ACTGCCTCAC GGTCAACCGC 
TCCCTGTTCC TCTTCAGTGA AGACAACGTG GTGAGAAAAT ACGCCAAAAA GATCACGGAA 
TGGCCTCCCT TCGAGTACAT GATCCTGGCC ACCATCATTG CTAACTGCAT CGTCCTGGCC 
CTGGAGCAGC ACCTCCCTGA TGATGACAAG ACACCCATGT CCGAGCGGCT GGATGACACA 
GAACCCTATT TCATTGGCAT CTTCTGTTTT GAGGCTGGAA TTAAGATCGT GGCTCTTGGC 
TTTGCCTTCC ACAAAGGCTC CTACCTGAGG AATGGCTGGA ACGTCATGGA CTTTGTCGTG 
GTGCTAACAG GCATCTTGGC CACTGTCGGG ACGGAGTTTG ATCTACGGAC ACTGAGGGCG 
GTTCGTGTGC TGCGGCCACT CAAGCTGGTG TCTGGAATCC CAAGTTTACA AGTCGTCCTG 
AAGTCAATCA TGAAGGCGAT GATCCCTCTG CTGCAGATCG GCCTCCTCCT GTTTTTTGCA 
ATCCTTATTT TTGCAATCAT AGGGTTAGAA TTTTATATGG GAAAATTTCA TACCACCTGC 
TTTGAAGAGG GGACAGACGA CATCCAGGGT GAGTCGCCAG CTCCGTGTGG GACAGAGGAG 
CCTGCCCGCA CCTGCCCCAA CGGGACCAAA TGTCAGCCGT ACTGGGAAGG GCCCAACAAC 
GGCATCACTC AGTTCGACAA CATCCTGTTT GCTGTGCTCA CTGTTTTCCA GTGCATCACC 
ATGGAAGGCT GGACTGATCT CCTCTACAAT AGCAACGATG CCTCAGGGAA CACTTGGAAC 
TGGTTGTACT TCATCCCCCT CATCATCATC GGCTCCTTTT TTATGCTGAA CCTTGTGCTG 
GGTGTGCTGT CTGGGGAGTT TGCCAAAGAA AGGGAACGTG TAGAGAACCG AAGGGCTTTT 
CTGAAGCTCA GAAGACAACA GCAGATTGAA CGTGAGCTCA ATGGATACAT GGAGTGGATC 
TCGAAAGCAG AAGAGGTGAT CCTCGCGGAG GACGAGACAG ACGTGGAGCA GAGGCACCCT 
TTTGATGGAG CTCTTCGGAG AGCTACTCTG AAGAAAAGCA AGACGGACCT GCTCAACCCT 
GAGGAGGCGG AGGACCAGCT TGCTGACATC GCGTCTGTGG GGTCTCCCTT CGCCAGAGCC 
AGCATCAAAA GTGCCAAGCT GGAGAATTCG ACTTTTTTCC ACAAAAAGGA GAGAAGAATG 
CGTTTCTACA TCCGCCGCAT GGTCAAAACT CAGGCCTTCT ACTGGACCGT GCTCAGTCTG 
GTAGCCCTCA ACACGCTGTG CGTCGCCATT GTCCACTACA ACCAGCCCGA GTGGCTCTCC 
GACTTCCTCT ACTATGCAGA ATTCATTTTC TTAGGACTCT TTATGTCCGA AATGTTTATA 
AAAATGTATG GGCTCGGGAC ACGGCCTTAC TTCCACTCTT CCTTCAACTG CTTTGACTGT 
GGGGTCATCA TCGGGAGCAT CTTTGAAGTC ATCTGGGCCG TCATCAAACC GGGTACATCC 
TTTGGAATCA GCGTGTTACG AGCTCTCAGG TTACTGCGTA TTTTCAAAGT CACAAAGTAC 
TGGGCATCTC TCAGAAACCT GGTTGTCTCC CTCCTCAACT CCATGAAATC CATCATAAGT 
CTGCTGTTCC TCCTCTTCCT CTTCATTGTC GTCTTTGCCC TCTTGGGGAT GCAGCTGTTT 
GGTGGCCAGT TTAATTTTGA CGAGGGGACT CCTCCCACCA ACTTCGACAC TTTTCCAGCA 
GCAATAATGA CTGTGTTTCA GATCCTGACT GGCGAGGATT GGAATGAGGT CATGTATGAT 
GGGATCAAGT CTCAGGGGGG CGTGCAGGGC GGCATGGTGT TCTCCATCTA CTTCATCGTC 
CTCACCCTCT TCGGGAACTA CACCCTGCTG AACGTGTTCT TGGCTATCGC GGTGGACAAC 
CTGGCCAACG CCCAGGAACT CACCAAGGAT GAACAAGAAG AGGAAGAGGC AGCCAATCAG 
AAACTGGCTC TACAGAAAGC CAAGGAGGTG GCAGAAGTGA GTCCCCTGTC TGCAGCCAAC 
ATGTCCATAG CTGTGAAGGA ACAGCAGAAG AACCAGAAGC CTGCCAAGTC GGTGTGGGAG 
CAGCGCACCA GCGAGATGCG CAAGCAGAAC CTGCTGGCTA GCCGCGAGGC GCTGTACGGG 
GACGCGGCTG AGCGCTGGCC CACCACTTAC GCGCGCCCGC TGCGGCCGGA CGTGAAGACG 
CACTTGGACC GGCCGCTCGT GGTGGACCCG CAGGAGAACC GTAACAACAA CACCAACAAG 
AGCCGTGCGC CAGAAGCGCT GCGCCAAACC GCGCGGCCCC GCGAGAGCGC GCGCGACCCC 
GACGCGCGGC GCGCCTGGCC CAGCAGCCCT GAGCGCGCCC CTGGACGAGA GGGCCCGTAT 
GGCCGCGAGA GCGAGCCGCA ACAGCGCGAG CACGCGCCAC CCCGCGAGCA CGTACCCTGG 
GACGCGGATC CTGAGCGCGC CAAGGCCGGG GACGCGCCCC GCCGCCACAC GCACCGGCCT 
GTGGCCGAGG GCGAGCCTCG TCGCCACCGC GCGCGCCGCC GGCCCGGGGA CGAACCGGAC 
GACAGACCGG AGCGCAGGCC GCGTCCCCGC GACGCCACTA GGCCGGCCCG CGCTGCAGAC 
GGCGAAGGCG ATGATGGGGA GCGCAAGCGG CGACACCGAC ACGGGCCGCC GGCCCACGAT 
GACAGGGAGC GCAGACACCG GCGCAGAAAA GAGAGCCAGG GCTCTGGGGT CCCCATGTCT 
GGTCCCAACC TGTCCACCAC CAGGCCAATC CAGCAGGATC TGGGCCGCCA GGACCTGCCA 
CTGGCTGAGG ACCTGGACAA CATGAAGAAC AACAAGTTGG CCACCGGGGA GCCTGCCAGT 
CCCCACGACA GCCTGGGCCA CAGTGGCCTT CCCCCTAGCC CTGCCAAGAT CGGGAACAGC 
ACCAACCCTG GTCCCGCCTT GGCCACCAAT CCCCAGAATG CTGCCAGCCG CAGGACGCCC 
AACAACCCGG GCAACCCGTC CAACCCCGGC CCCCCCAAGA CTCCCGAGAA CAGCCTTATC 
GTCACCAACC CCAGCAGCAC CCAGCCCAAC TCAGCAAAGA CTGCCAGGAA ACCCGAGCAC 
ATGGCGGTGG AGATCCCCCC GGCCTGCCCG CCCCTCAACC ACACTGTGGT CCAAGTAAAC 
AAAAACGCCA ACCCAGACCC ACTGCCAAAG AAAGAGGAAG AGAAGAAGGA GGAAGAGGAG 
GCAGACCCCG GGGAGGATGG CCCAAAGCCC ATGCCGCCCT ACAGCTCCAT GTTCATCCTC 
TCCACCACCA ACCCCCTTCG CCGGCTGTGC CATTACATCC TGAACCTGCG CTACTTCGAG 
ATGTGCATCC TCATGGTCAT TGCCATGAGT AGCATCGCGC TGGCCGCCGA GGACCCGGTG 
CAGCCCAACG CACCCCGCAA CAACGTGCTG CGATATTTTG ACTATGTTTT CACAGGAGTG 
TTTACCTTTG AGATGGTGAT CAAGATGATC GACCTGGGCC TCGTCCTGCA TCAGGGGGCC 
TATTTCCGTG ACCTGTGGAA CATTCTGGAC TTCATAGTGG TCAGTGGGGC CCTGGTGGCC 
TTTGCCTTCA CTGGCAATAG CAAAGGAAAG GACATCAACA CCATCAAGTC CCTCCGAGTC 
CTCCGGGTGC TACGACCTCT AAAGACCATC AAGCGGCTGC CTAAGTTGAA GGCCGTATTT 
GACTGCGTGG TGAACTCGCT CAAGAACGTC TTCAACATCC TCATTGTCTA CATGCTCTTC 
ATGTTCATCT TCGCCGTGGT GGCCGTGCAG CTCTTCAAGG GCAAATTCTT CCACTGCACG 
GACGAGTCCA AGGAGTTTGA GAGAGACTGT CGAGGCAAAT ACCTCCTTTA CGAGAAGAAC 
GAGGTAAAGG CGCGGGACCG CGAGTGGAAG AAATACGACT TCCACTACGA CAACGTGCTC 
TGGGCCCTGC TCACGCTCTT TACGGTGTCC ACGGGAGAGG GCTGGCCACA GGTCCTCAAG 
CACTCAGTGG ATGCCACTTT TGAGAACCAG GGCCCCAGCC CCGGGTACCG CATGGAAATG 
TCCATCTTCT ACGTGGTCTA CTTTGTGGTG TTTCCCTTCT TCTTTGTCAA TATCTTTGTG 
GCCTTGATCA TCATCACCTT CCAGGAGCAG GGAGACAAGA TGATGGAAGA ATACAGCCTA 
GAGAAAAATG AGAGGGCCTG CATCGACTTT GCCATCAGTG CCAAGCCGCT GACCAGGCAC 
ATGCCCCAGA ACAAGCAGAG CTTCCAGTAT CGAATGTGGC AGTTCGTGGT GTCCCCACCC 
TTTGAGTACA CCATCATGGC CATGATCGCT CTCAACACCA TCGTGCTAAT GATGAAGTTC 
TATGGAGCCT CTGTGGCCTA TGAAAACGCC CTTCGAGTGT TCAACATTGT CTTCACCTCC 
CTCTTCTCTC TCGAATGTGT GCTCAAAGTC ATGGCTTTTG GGATTCTGAA TTATTTCCGC 
GATGCCTGGA ACATCTTCGA CTTTGTGACT GTTCTGGGCA GCATCACAGA CATCCTCGTC 
ACCGAGTTTG GGAATAACTT CATCAACCTG AGCTTTCTCC GCCTCTTCCG TGCTGCCCGA 
CTCATCAAAC TCCTCCGCCA GGGTTACACC ATCCGCATTC TCCTCTGGAC TTTCGTGCAG 
TCTTTCAAGG CCCTACCTTA TGTCTGTCTG CTGATCGCCA TGCTCTTCTT CATCTATGCC 
ATCATCGGGA TGCAGGTGTT TGGCAACATC GGCATTGATG GGGAAGATGA GGACAGCGAT 
GAGGATGAGT TCCAAATCAC GGAGCACAAT AACTTCCGGA CCTTCTTCCA AGCTCTCATG 
CTTCTCTTCC GGAGCGCCAC AGGGGAAGCG TGGCACAACA TCATGCTGTC CTGCCTCAGC 
GGGAAGCCAT GCGACAAGAA CTCCGGGATC CAAAAACCAG AGTGTGGCAA CGAGTTCGCC 
TATTTTTACT TTGTCTCGTT CATCTTCCTT TGCTCATTTC TGATGCTGAA TCTCTTTGTT 
GCTGTCATCA TGGACAACTT CGAGTACCTC ACCCGAGATT CCTCCATCCT GGGCCCCCAC 
CACCTGGATG AGTACGTGCG TGTCTGGGCA GAGTATGACC CTGCTGCCTG CGGCCGCATT 
CACTATAAGG ACATGTACAG TTTATTGCGA GTAATATCGC CCCCTCTCGG CTTAGGCAAG 
AAATGTCCTC ATAGGGTTGC TTGCAAGAGG CTCTTGCGGA TGGACCTACC CGTAGCGGAT 
GACAACACCG TTCACTTCAA CTCCACCTTG ATGGCTCTGA TCCGAACCGC CCTGGATATC 
AAAATCGCAA AGGGTGGAGC TGACAAGCAG CAAATGGACG CAGAGCTCCG CAAGGAAATG 
ATGGCCATTT GGCCCAACCT GTCTCAGAAG ACCTTGGATC TGCTGGTCAC ACCTCACAAG 
TCCACGGACC TGACAGTGGG TAAGATCTAC GCAGCCATGA TGATCATGGA GTACTACCGG 
CAGAGCAAGG CCAAGAAGCT GCAGGCCATG CGAGAGGAGC AGAACCGGAC ACCACTCATG 
TTCCAGCGCA TGGAGCCTCC ATCGCCAACA CAGGAGGGAG GACCCAGCCA AAACGCCCTT 
CCCTCCACTC AGCTGGACCC CGGAGGAGGC CTGATGGCTC AAGAAAGCAG CATGAAGGAG 
AGCCCGTCCT GGGTGACCCA GCGGGCACAG GAGATGTTCC AGAAGACTGG TACCTGGAGC 
CCAGAGCGAG GGCCACCCAT CGACATGCCT AACAGCCAGC CCAACTCCCA GTCTGTGGAG 
ATGAGAGAAA TGGGAACTGA TGGCTACTCA GACAGCGAAC ACTACCTCCC CATGGAAGGA 
CAGACCAGGG CCGCCTCCAT GCCCCGCCTC CCAGCAGAGA ACCAGAGGAG AAGGGGCCGG 
CCACGTGGAA ATAACCTCAG TACCATCTCT GATACCAGCC CCATGAAGCG CTCAGCCTCC 
GTGCTGGGAC CCAAAGCCCG GCGACTGGAT GACTACTCAC TAGAGCGGGT ACCACCTGAG 
GAGAACCAAA GGTACCACCA ACGCCGCCGG GACCGTGGCC ACCGCACCTC TGAGCGCTCT 
CTGGGCCGAT ACACTGATGT GGACACAGGC CTGGGGACAG ATCTGAGCAT GACCACCCAA 
TCGGGTGACC TGCCCTCCAA AGATCGGGAC CAGGACCGGG GCCGGCCCAA GGACCGGAAG 
CATCGGCCAC ACCACCACCA CCACCATCAT CACCATCATC CCCCGGCCCC GGACCGGGAG 
CGCTACGCAC AGGAGCGGCC GGACACCGGC CGGGCGCGGG CCCGGGAGCA GCGCTGGTCC 
CGCTCGCCCA GCGAGGGTCG GGAGCACGCG ACACACAGAC AGGGCAGTAG TTCCGTCAGT 
GGAAGCCCAG CCCCCTCTAC GTCCGGCACC AGCACGCCGC GGCGCGGCCG CCGCCAGCTC 
CCCCAGACCC CCTGCACCCC GCGGCCGCTT GTGTCCTACT CGCCCGCTCC GCGCAGGCCT 
GCGGCGCGCA GGATGGCGGG TCCCCCAGCG CCCCCTGGCG GCTCGCCGCG GGGTTGCCGC 
CGTGCGCCGC GCTGGCCTGC GCATGCGCCT GAGGGCCCGC GGCCGCGCGG TGCCGACTAC 
ACCGAGCCCG ACAGCCCGCG CGAGCCGCCA GGGGGCGCGC ATGAGCCCGC GCCGCGGTCG 
CCCAGGACTC CGCGCGCCGC GGGCTGCGCG TCACCGCGCC ACGGCCGCAG GCTGCCCAAT 
GGTTACTACG CGGGGCACGG CGCGCCACGG CCGCGCACAG CCCGCAGGGG CGCGCACGAC 
GCGTACAGCG AGAGCGAGGA CGACTGGTGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017456671.1
CDS	105..7517
Translation	MVSSPGISERGPWMPRASWVSDPMRSPLNHELLHSSRYPGTDCTLAGSSRTLHVPWSEQMSGSLEGPTLKRRGGVVDHRDVILAHQAHKMHSTPQARRKEWEMARFGDEMPGRYGAGGGGSGPAAGVVVGAAGGRGAGGSRQGGQPGAQRMYKQSMAQRARTMALYNPIPVRQNCLTVNRSLFLFSEDNVVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDDDKTPMSERLDDTEPYFIGIFCFEAGIKIVALGFAFHKGSYLRNGWNVMDFVVVLTGILATVGTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILIFAIIGLEFYMGKFHTTCFEEGTDDIQGESPAPCGTEEPARTCPNGTKCQPYWEGPNNGITQFDNILFAVLTVFQCITMEGWTDLLYNSNDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYMEWISKAEEVILAEDETDVEQRHPFDGALRRATLKKSKTDLLNPEEAEDQLADIASVGSPFARASIKSAKLENSTFFHKKERRMRFYIRRMVKTQAFYWTVLSLVALNTLCVAIVHYNQPEWLSDFLYYAEFIFLGLFMSEMFIKMYGLGTRPYFHSSFNCFDCGVIIGSIFEVIWAVIKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFDEGTPPTNFDTFPAAIMTVFQILTGEDWNEVMYDGIKSQGGVQGGMVFSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAANQKLALQKAKEVAEVSPLSAANMSIAVKEQQKNQKPAKSVWEQRTSEMRKQNLLASREALYGDAAERWPTTYARPLRPDVKTHLDRPLVVDPQENRNNNTNKSRAPEALRQTARPRESARDPDARRAWPSSPERAPGREGPYGRESEPQQREHAPPREHVPWDADPERAKAGDAPRRHTHRPVAEGEPRRHRARRRPGDEPDDRPERRPRPRDATRPARAADGEGDDGERKRRHRHGPPAHDDRERRHRRRKESQGSGVPMSGPNLSTTRPIQQDLGRQDLPLAEDLDNMKNNKLATGEPASPHDSLGHSGLPPSPAKIGNSTNPGPALATNPQNAASRRTPNNPGNPSNPGPPKTPENSLIVTNPSSTQPNSAKTARKPEHMAVEIPPACPPLNHTVVQVNKNANPDPLPKKEEEKKEEEEADPGEDGPKPMPPYSSMFILSTTNPLRRLCHYILNLRYFEMCILMVIAMSSIALAAEDPVQPNAPRNNVLRYFDYVFTGVFTFEMVIKMIDLGLVLHQGAYFRDLWNILDFIVVSGALVAFAFTGNSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYMLFMFIFAVVAVQLFKGKFFHCTDESKEFERDCRGKYLLYEKNEVKARDREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATFENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEEYSLEKNERACIDFAISAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYTIMAMIALNTIVLMMKFYGASVAYENALRVFNIVFTSLFSLECVLKVMAFGILNYFRDAWNIFDFVTVLGSITDILVTEFGNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIGIDGEDEDSDEDEFQITEHNNFRTFFQALMLLFRSATGEAWHNIMLSCLSGKPCDKNSGIQKPECGNEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRVWAEYDPAACGRIHYKDMYSLLRVISPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKQQMDAELRKEMMAIWPNLSQKTLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKKLQAMREEQNRTPLMFQRMEPPSPTQEGGPSQNALPSTQLDPGGGLMAQESSMKESPSWVTQRAQEMFQKTGTWSPERGPPIDMPNSQPNSQSVEMREMGTDGYSDSEHYLPMEGQTRAASMPRLPAENQRRRGRPRGNNLSTISDTSPMKRSASVLGPKARRLDDYSLERVPPEENQRYHQRRRDRGHRTSERSLGRYTDVDTGLGTDLSMTTQSGDLPSKDRDQDRGRPKDRKHRPHHHHHHHHHHPPAPDRERYAQERPDTGRARAREQRWSRSPSEGREHATHRQGSSSVSGSPAPSTSGTSTPRRGRRQLPQTPCTPRPLVSYSPAPRRPAARRMAGPPAPPGGSPRGCRRAPRWPAHAPEGPRPRGADYTEPDSPREPPGGAHEPAPRSPRTPRAAGCASPRHGRRLPNGYYAGHGAPRPRTARRGAHDAYSESEDDWC

Target ORF information:

RefSeq Version	XM_017601182.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel subunit alpha1 A (Cacna1a), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017601182.1

ORF Insert Sequence:

ATGGTTTCCT CTCCTGGGAT TTCTGAGCGA GGCCCATGGA TGCCGAGGGC CTCCTGGGTC 
TCTGACCCCA TGCGTTCTCC CCTCAATCAT GAGCTCCTGC ACAGCTCGAG GTACCCAGGC 
ACGGACTGCA CCCTGGCAGG TTCTTCCAGG ACTTTGCATG TCCCGTGGTC CGAGCAGATG 
AGCGGAAGCC TCGAGGGGCC AACTCTGAAA CGGCGTGGTG GCGTGGTGGA CCACCGGGAT 
GTCATCTTGG CCCACCAGGC GCACAAAATG CACAGCACCC CTCAGGCCAG GAGGAAAGAG 
TGGGAAATGG CCCGCTTTGG AGACGAGATG CCGGGCCGCT ACGGCGCAGG CGGAGGAGGC 
TCAGGGCCGG CCGCCGGGGT GGTCGTGGGC GCCGCGGGCG GCCGAGGAGC CGGGGGCAGC 
CGGCAGGGCG GGCAGCCCGG AGCGCAGAGG ATGTACAAGC AGTCGATGGC GCAGAGAGCG 
CGGACCATGG CCCTCTACAA CCCCATCCCT GTCCGCCAGA ACTGCCTCAC GGTCAACCGC 
TCCCTGTTCC TCTTCAGTGA AGACAACGTG GTGAGAAAAT ACGCCAAAAA GATCACGGAA 
TGGCCTCCCT TCGAGTACAT GATCCTGGCC ACCATCATTG CTAACTGCAT CGTCCTGGCC 
CTGGAGCAGC ACCTCCCTGA TGATGACAAG ACACCCATGT CCGAGCGGCT GGATGACACA 
GAACCCTATT TCATTGGCAT CTTCTGTTTT GAGGCTGGAA TTAAGATCGT GGCTCTTGGC 
TTTGCCTTCC ACAAAGGCTC CTACCTGAGG AATGGCTGGA ACGTCATGGA CTTTGTCGTG 
GTGCTAACAG GCATCTTGGC CACTGTCGGG ACGGAGTTTG ATCTACGGAC ACTGAGGGCG 
GTTCGTGTGC TGCGGCCACT CAAGCTGGTG TCTGGAATCC CAAGTTTACA AGTCGTCCTG 
AAGTCAATCA TGAAGGCGAT GATCCCTCTG CTGCAGATCG GCCTCCTCCT GTTTTTTGCA 
ATCCTTATTT TTGCAATCAT AGGGTTAGAA TTTTATATGG GAAAATTTCA TACCACCTGC 
TTTGAAGAGG GGACAGACGA CATCCAGGGT GAGTCGCCAG CTCCGTGTGG GACAGAGGAG 
CCTGCCCGCA CCTGCCCCAA CGGGACCAAA TGTCAGCCGT ACTGGGAAGG GCCCAACAAC 
GGCATCACTC AGTTCGACAA CATCCTGTTT GCTGTGCTCA CTGTTTTCCA GTGCATCACC 
ATGGAAGGCT GGACTGATCT CCTCTACAAT AGCAACGATG CCTCAGGGAA CACTTGGAAC 
TGGTTGTACT TCATCCCCCT CATCATCATC GGCTCCTTTT TTATGCTGAA CCTTGTGCTG 
GGTGTGCTGT CTGGGGAGTT TGCCAAAGAA AGGGAACGTG TAGAGAACCG AAGGGCTTTT 
CTGAAGCTCA GAAGACAACA GCAGATTGAA CGTGAGCTCA ATGGATACAT GGAGTGGATC 
TCGAAAGCAG AAGAGGTGAT CCTCGCGGAG GACGAGACAG ACGTGGAGCA GAGGCACCCT 
TTTGATGGAG CTCTTCGGAG AGCTACTCTG AAGAAAAGCA AGACGGACCT GCTCAACCCT 
GAGGAGGCGG AGGACCAGCT TGCTGACATC GCGTCTGTGG GGTCTCCCTT CGCCAGAGCC 
AGCATCAAAA GTGCCAAGCT GGAGAATTCG ACTTTTTTCC ACAAAAAGGA GAGAAGAATG 
CGTTTCTACA TCCGCCGCAT GGTCAAAACT CAGGCCTTCT ACTGGACCGT GCTCAGTCTG 
GTAGCCCTCA ACACGCTGTG CGTCGCCATT GTCCACTACA ACCAGCCCGA GTGGCTCTCC 
GACTTCCTCT ACTATGCAGA ATTCATTTTC TTAGGACTCT TTATGTCCGA AATGTTTATA 
AAAATGTATG GGCTCGGGAC ACGGCCTTAC TTCCACTCTT CCTTCAACTG CTTTGACTGT 
GGGGTCATCA TCGGGAGCAT CTTTGAAGTC ATCTGGGCCG TCATCAAACC GGGTACATCC 
TTTGGAATCA GCGTGTTACG AGCTCTCAGG TTACTGCGTA TTTTCAAAGT CACAAAGTAC 
TGGGCATCTC TCAGAAACCT GGTTGTCTCC CTCCTCAACT CCATGAAATC CATCATAAGT 
CTGCTGTTCC TCCTCTTCCT CTTCATTGTC GTCTTTGCCC TCTTGGGGAT GCAGCTGTTT 
GGTGGCCAGT TTAATTTTGA CGAGGGGACT CCTCCCACCA ACTTCGACAC TTTTCCAGCA 
GCAATAATGA CTGTGTTTCA GATCCTGACT GGCGAGGATT GGAATGAGGT CATGTATGAT 
GGGATCAAGT CTCAGGGGGG CGTGCAGGGC GGCATGGTGT TCTCCATCTA CTTCATCGTC 
CTCACCCTCT TCGGGAACTA CACCCTGCTG AACGTGTTCT TGGCTATCGC GGTGGACAAC 
CTGGCCAACG CCCAGGAACT CACCAAGGAT GAACAAGAAG AGGAAGAGGC AGCCAATCAG 
AAACTGGCTC TACAGAAAGC CAAGGAGGTG GCAGAAGTGA GTCCCCTGTC TGCAGCCAAC 
ATGTCCATAG CTGTGAAGGA ACAGCAGAAG AACCAGAAGC CTGCCAAGTC GGTGTGGGAG 
CAGCGCACCA GCGAGATGCG CAAGCAGAAC CTGCTGGCTA GCCGCGAGGC GCTGTACGGG 
GACGCGGCTG AGCGCTGGCC CACCACTTAC GCGCGCCCGC TGCGGCCGGA CGTGAAGACG 
CACTTGGACC GGCCGCTCGT GGTGGACCCG CAGGAGAACC GTAACAACAA CACCAACAAG 
AGCCGTGCGC CAGAAGCGCT GCGCCAAACC GCGCGGCCCC GCGAGAGCGC GCGCGACCCC 
GACGCGCGGC GCGCCTGGCC CAGCAGCCCT GAGCGCGCCC CTGGACGAGA GGGCCCGTAT 
GGCCGCGAGA GCGAGCCGCA ACAGCGCGAG CACGCGCCAC CCCGCGAGCA CGTACCCTGG 
GACGCGGATC CTGAGCGCGC CAAGGCCGGG GACGCGCCCC GCCGCCACAC GCACCGGCCT 
GTGGCCGAGG GCGAGCCTCG TCGCCACCGC GCGCGCCGCC GGCCCGGGGA CGAACCGGAC 
GACAGACCGG AGCGCAGGCC GCGTCCCCGC GACGCCACTA GGCCGGCCCG CGCTGCAGAC 
GGCGAAGGCG ATGATGGGGA GCGCAAGCGG CGACACCGAC ACGGGCCGCC GGCCCACGAT 
GACAGGGAGC GCAGACACCG GCGCAGAAAA GAGAGCCAGG GCTCTGGGGT CCCCATGTCT 
GGTCCCAACC TGTCCACCAC CAGGCCAATC CAGCAGGATC TGGGCCGCCA GGACCTGCCA 
CTGGCTGAGG ACCTGGACAA CATGAAGAAC AACAAGTTGG CCACCGGGGA GCCTGCCAGT 
CCCCACGACA GCCTGGGCCA CAGTGGCCTT CCCCCTAGCC CTGCCAAGAT CGGGAACAGC 
ACCAACCCTG GTCCCGCCTT GGCCACCAAT CCCCAGAATG CTGCCAGCCG CAGGACGCCC 
AACAACCCGG GCAACCCGTC CAACCCCGGC CCCCCCAAGA CTCCCGAGAA CAGCCTTATC 
GTCACCAACC CCAGCAGCAC CCAGCCCAAC TCAGCAAAGA CTGCCAGGAA ACCCGAGCAC 
ATGGCGGTGG AGATCCCCCC GGCCTGCCCG CCCCTCAACC ACACTGTGGT CCAAGTAAAC 
AAAAACGCCA ACCCAGACCC ACTGCCAAAG AAAGAGGAAG AGAAGAAGGA GGAAGAGGAG 
GCAGACCCCG GGGAGGATGG CCCAAAGCCC ATGCCGCCCT ACAGCTCCAT GTTCATCCTC 
TCCACCACCA ACCCCCTTCG CCGGCTGTGC CATTACATCC TGAACCTGCG CTACTTCGAG 
ATGTGCATCC TCATGGTCAT TGCCATGAGT AGCATCGCGC TGGCCGCCGA GGACCCGGTG 
CAGCCCAACG CACCCCGCAA CAACGTGCTG CGATATTTTG ACTATGTTTT CACAGGAGTG 
TTTACCTTTG AGATGGTGAT CAAGATGATC GACCTGGGCC TCGTCCTGCA TCAGGGGGCC 
TATTTCCGTG ACCTGTGGAA CATTCTGGAC TTCATAGTGG TCAGTGGGGC CCTGGTGGCC 
TTTGCCTTCA CTGGCAATAG CAAAGGAAAG GACATCAACA CCATCAAGTC CCTCCGAGTC 
CTCCGGGTGC TACGACCTCT AAAGACCATC AAGCGGCTGC CTAAGTTGAA GGCCGTATTT 
GACTGCGTGG TGAACTCGCT CAAGAACGTC TTCAACATCC TCATTGTCTA CATGCTCTTC 
ATGTTCATCT TCGCCGTGGT GGCCGTGCAG CTCTTCAAGG GCAAATTCTT CCACTGCACG 
GACGAGTCCA AGGAGTTTGA GAGAGACTGT CGAGGCAAAT ACCTCCTTTA CGAGAAGAAC 
GAGGTAAAGG CGCGGGACCG CGAGTGGAAG AAATACGACT TCCACTACGA CAACGTGCTC 
TGGGCCCTGC TCACGCTCTT TACGGTGTCC ACGGGAGAGG GCTGGCCACA GGTCCTCAAG 
CACTCAGTGG ATGCCACTTT TGAGAACCAG GGCCCCAGCC CCGGGTACCG CATGGAAATG 
TCCATCTTCT ACGTGGTCTA CTTTGTGGTG TTTCCCTTCT TCTTTGTCAA TATCTTTGTG 
GCCTTGATCA TCATCACCTT CCAGGAGCAG GGAGACAAGA TGATGGAAGA ATACAGCCTA 
GAGAAAAATG AGAGGGCCTG CATCGACTTT GCCATCAGTG CCAAGCCGCT GACCAGGCAC 
ATGCCCCAGA ACAAGCAGAG CTTCCAGTAT CGAATGTGGC AGTTCGTGGT GTCCCCACCC 
TTTGAGTACA CCATCATGGC CATGATCGCT CTCAACACCA TCGTGCTAAT GATGAAGTTC 
TATGGAGCCT CTGTGGCCTA TGAAAACGCC CTTCGAGTGT TCAACATTGT CTTCACCTCC 
CTCTTCTCTC TCGAATGTGT GCTCAAAGTC ATGGCTTTTG GGATTCTGAA TTATTTCCGC 
GATGCCTGGA ACATCTTCGA CTTTGTGACT GTTCTGGGCA GCATCACAGA CATCCTCGTC 
ACCGAGTTTG GGAATAACTT CATCAACCTG AGCTTTCTCC GCCTCTTCCG TGCTGCCCGA 
CTCATCAAAC TCCTCCGCCA GGGTTACACC ATCCGCATTC TCCTCTGGAC TTTCGTGCAG 
TCTTTCAAGG CCCTACCTTA TGTCTGTCTG CTGATCGCCA TGCTCTTCTT CATCTATGCC 
ATCATCGGGA TGCAGGTGTT TGGCAACATC GGCATTGATG GGGAAGATGA GGACAGCGAT 
GAGGATGAGT TCCAAATCAC GGAGCACAAT AACTTCCGGA CCTTCTTCCA AGCTCTCATG 
CTTCTCTTCC GGAGCGCCAC AGGGGAAGCG TGGCACAACA TCATGCTGTC CTGCCTCAGC 
GGGAAGCCAT GCGACAAGAA CTCCGGGATC CAAAAACCAG AGTGTGGCAA CGAGTTCGCC 
TATTTTTACT TTGTCTCGTT CATCTTCCTT TGCTCATTTC TGATGCTGAA TCTCTTTGTT 
GCTGTCATCA TGGACAACTT CGAGTACCTC ACCCGAGATT CCTCCATCCT GGGCCCCCAC 
CACCTGGATG AGTACGTGCG TGTCTGGGCA GAGTATGACC CTGCTGCCTG CGGCCGCATT 
CACTATAAGG ACATGTACAG TTTATTGCGA GTAATATCGC CCCCTCTCGG CTTAGGCAAG 
AAATGTCCTC ATAGGGTTGC TTGCAAGAGG CTCTTGCGGA TGGACCTACC CGTAGCGGAT 
GACAACACCG TTCACTTCAA CTCCACCTTG ATGGCTCTGA TCCGAACCGC CCTGGATATC 
AAAATCGCAA AGGGTGGAGC TGACAAGCAG CAAATGGACG CAGAGCTCCG CAAGGAAATG 
ATGGCCATTT GGCCCAACCT GTCTCAGAAG ACCTTGGATC TGCTGGTCAC ACCTCACAAG 
TCCACGGACC TGACAGTGGG TAAGATCTAC GCAGCCATGA TGATCATGGA GTACTACCGG 
CAGAGCAAGG CCAAGAAGCT GCAGGCCATG CGAGAGGAGC AGAACCGGAC ACCACTCATG 
TTCCAGCGCA TGGAGCCTCC ATCGCCAACA CAGGAGGGAG GACCCAGCCA AAACGCCCTT 
CCCTCCACTC AGCTGGACCC CGGAGGAGGC CTGATGGCTC AAGAAAGCAG CATGAAGGAG 
AGCCCGTCCT GGGTGACCCA GCGGGCACAG GAGATGTTCC AGAAGACTGG TACCTGGAGC 
CCAGAGCGAG GGCCACCCAT CGACATGCCT AACAGCCAGC CCAACTCCCA GTCTGTGGAG 
ATGAGAGAAA TGGGAACTGA TGGCTACTCA GACAGCGAAC ACTACCTCCC CATGGAAGGA 
CAGACCAGGG CCGCCTCCAT GCCCCGCCTC CCAGCAGAGA ACCAGAGGAG AAGGGGCCGG 
CCACGTGGAA ATAACCTCAG TACCATCTCT GATACCAGCC CCATGAAGCG CTCAGCCTCC 
GTGCTGGGAC CCAAAGCCCG GCGACTGGAT GACTACTCAC TAGAGCGGGT ACCACCTGAG 
GAGAACCAAA GGTACCACCA ACGCCGCCGG GACCGTGGCC ACCGCACCTC TGAGCGCTCT 
CTGGGCCGAT ACACTGATGT GGACACAGGC CTGGGGACAG ATCTGAGCAT GACCACCCAA 
TCGGGTGACC TGCCCTCCAA AGATCGGGAC CAGGACCGGG GCCGGCCCAA GGACCGGAAG 
CATCGGCCAC ACCACCACCA CCACCATCAT CACCATCATC CCCCGGCCCC GGACCGGGAG 
CGCTACGCAC AGGAGCGGCC GGACACCGGC CGGGCGCGGG CCCGGGAGCA GCGCTGGTCC 
CGCTCGCCCA GCGAGGGTCG GGAGCACGCG ACACACAGAC AGGGCAGTAG TTCCGTCAGT 
GGAAGCCCAG CCCCCTCTAC GTCCGGCACC AGCACGCCGC GGCGCGGCCG CCGCCAGCTC 
CCCCAGACCC CCTGCACCCC GCGGCCGCTT GTGTCCTACT CGCCCGCTCC GCGCAGGCCT 
GCGGCGCGCA GGATGGCGGG TCCCCCAGCG CCCCCTGGCG GCTCGCCGCG GGGTTGCCGC 
CGTGCGCCGC GCTGGCCTGC GCATGCGCCT GAGGGCCCGC GGCCGCGCGG TGCCGACTAC 
ACCGAGCCCG ACAGCCCGCG CGAGCCGCCA GGGGGCGCGC ATGAGCCCGC GCCGCGGTCG 
CCCAGGACTC CGCGCGCCGC GGGCTGCGCG TCACCGCGCC ACGGCCGCAG GCTGCCCAAT 
GGTTACTACG CGGGGCACGG CGCGCCACGG CCGCGCACAG CCCGCAGGGG CGCGCACGAC 
GCGTACAGCG AGAGCGAGGA CGACTGGTGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1a ( XM_008772359.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008770581.2 Rattus norvegicus calcium voltage-gated channel subunit alpha1 A (Cacna1a), transcript variant X15, mRNA.

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CloneID	ORa59378
Clone ID Related Accession (Same CDS sequence)	XM_008772359.2
Accession Version	XM_008772359.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008772359.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTCCT CTCCTGGGAT TTCTGAGCGA GGCCCATGGA TGCCGAGGGC CTCCTGGGTC 
TCTGACCCCA TGCGTTCTCC CCTCAATCAT GAGCTCCTGC ACAGCTCGAG GTACCCAGGC 
ACGGACTGCA CCCTGGCAGG TTCTTCCAGG ACTTTGCATG TCCCGTGGTC CGAGCAGATG 
AGCGGAAGCC TCGAGGGGCC AACTCTGAAA CGGCGTGGTG GCGTGGTGGA CCACCGGGAT 
GTCATCTTGG CCCACCAGGC GCACAAAATG CACAGCACCC CTCAGGCCAG GAGGAAAGAG 
TGGGAAATGG CCCGCTTTGG AGACGAGATG CCGGGCCGCT ACGGCGCAGG CGGAGGAGGC 
TCAGGGCCGG CCGCCGGGGT GGTCGTGGGC GCCGCGGGCG GCCGAGGAGC CGGGGGCAGC 
CGGCAGGGCG GGCAGCCCGG AGCGCAGAGG ATGTACAAGC AGTCGATGGC GCAGAGAGCG 
CGGACCATGG CCCTCTACAA CCCCATCCCT GTCCGCCAGA ACTGCCTCAC GGTCAACCGC 
TCCCTGTTCC TCTTCAGTGA AGACAACGTG GTGAGAAAAT ACGCCAAAAA GATCACGGAA 
TGGCCTCCCT TCGAGTACAT GATCCTGGCC ACCATCATTG CTAACTGCAT CGTCCTGGCC 
CTGGAGCAGC ACCTCCCTGA TGATGACAAG ACACCCATGT CCGAGCGGCT GGATGACACA 
GAACCCTATT TCATTGGCAT CTTCTGTTTT GAGGCTGGAA TTAAGATCGT GGCTCTTGGC 
TTTGCCTTCC ACAAAGGCTC CTACCTGAGG AATGGCTGGA ACGTCATGGA CTTTGTCGTG 
GTGCTAACAG GCATCTTGGC CACTGTCGGG ACGGAGTTTG ATCTACGGAC ACTGAGGGCG 
GTTCGTGTGC TGCGGCCACT CAAGCTGGTG TCTGGAATCC CAAGTTTACA AGTCGTCCTG 
AAGTCAATCA TGAAGGCGAT GATCCCTCTG CTGCAGATCG GCCTCCTCCT GTTTTTTGCA 
ATCCTTATTT TTGCAATCAT AGGGTTAGAA TTTTATATGG GAAAATTTCA TACCACCTGC 
TTTGAAGAGG GGACAGACGA CATCCAGGGT GAGTCGCCAG CTCCGTGTGG GACAGAGGAG 
CCTGCCCGCA CCTGCCCCAA CGGGACCAAA TGTCAGCCGT ACTGGGAAGG GCCCAACAAC 
GGCATCACTC AGTTCGACAA CATCCTGTTT GCTGTGCTCA CTGTTTTCCA GTGCATCACC 
ATGGAAGGCT GGACTGATCT CCTCTACAAT AGCAACGATG CCTCAGGGAA CACTTGGAAC 
TGGTTGTACT TCATCCCCCT CATCATCATC GGCTCCTTTT TTATGCTGAA CCTTGTGCTG 
GGTGTGCTGT CTGGGGAGTT TGCCAAAGAA AGGGAACGTG TAGAGAACCG AAGGGCTTTT 
CTGAAGCTCA GAAGACAACA GCAGATTGAA CGTGAGCTCA ATGGATACAT GGAGTGGATC 
TCGAAAGCAG AAGAGGTGAT CCTCGCGGAG GACGAGACAG ACGTGGAGCA GAGGCACCCT 
TTTGATGGAG CTCTTCGGAG AGCTACTCTG AAGAAAAGCA AGACGGACCT GCTCAACCCT 
GAGGAGGCGG AGGACCAGCT TGCTGACATC GCGTCTGTGG GGTCTCCCTT CGCCAGAGCC 
AGCATCAAAA GTGCCAAGCT GGAGAATTCG ACTTTTTTCC ACAAAAAGGA GAGAAGAATG 
CGTTTCTACA TCCGCCGCAT GGTCAAAACT CAGGCCTTCT ACTGGACCGT GCTCAGTCTG 
GTAGCCCTCA ACACGCTGTG CGTCGCCATT GTCCACTACA ACCAGCCCGA GTGGCTCTCC 
GACTTCCTCT ACTATGCAGA ATTCATTTTC TTAGGACTCT TTATGTCCGA AATGTTTATA 
AAAATGTATG GGCTCGGGAC ACGGCCTTAC TTCCACTCTT CCTTCAACTG CTTTGACTGT 
GGGGTCATCA TCGGGAGCAT CTTTGAAGTC ATCTGGGCCG TCATCAAACC GGGTACATCC 
TTTGGAATCA GCGTGTTACG AGCTCTCAGG TTACTGCGTA TTTTCAAAGT CACAAAGTAC 
TGGGCATCTC TCAGAAACCT GGTTGTCTCC CTCCTCAACT CCATGAAATC CATCATAAGT 
CTGCTGTTCC TCCTCTTCCT CTTCATTGTC GTCTTTGCCC TCTTGGGGAT GCAGCTGTTT 
GGTGGCCAGT TTAATTTTGA CGAGGGGACT CCTCCCACCA ACTTCGACAC TTTTCCAGCA 
GCAATAATGA CTGTGTTTCA GATCCTGACT GGCGAGGATT GGAATGAGGT CATGTATGAT 
GGGATCAAGT CTCAGGGGGG CGTGCAGGGC GGCATGGTGT TCTCCATCTA CTTCATCGTC 
CTCACCCTCT TCGGGAACTA CACCCTGCTG AACGTGTTCT TGGCTATCGC GGTGGACAAC 
CTGGCCAACG CCCAGGAACT CACCAAGGAT GAACAAGAAG AGGAAGAGGC AGCCAATCAG 
AAACTGGCTC TACAGAAAGC CAAGGAGGTG GCAGAAGTGA GTCCCCTGTC TGCAGCCAAC 
ATGTCCATAG CTGTGAAGGA ACAGCAGAAG AACCAGAAGC CTGCCAAGTC GGTGTGGGAG 
CAGCGCACCA GCGAGATGCG CAAGCAGAAC CTGCTGGCTA GCCGCGAGGC GCTGTACGGG 
GACGCGGCTG AGCGCTGGCC CACCACTTAC GCGCGCCCGC TGCGGCCGGA CGTGAAGACG 
CACTTGGACC GGCCGCTCGT GGTGGACCCG CAGGAGAACC GTAACAACAA CACCAACAAG 
AGCCGTGCGC CAGAAGCGCT GCGCCAAACC GCGCGGCCCC GCGAGAGCGC GCGCGACCCC 
GACGCGCGGC GCGCCTGGCC CAGCAGCCCT GAGCGCGCCC CTGGACGAGA GGGCCCGTAT 
GGCCGCGAGA GCGAGCCGCA ACAGCGCGAG CACGCGCCAC CCCGCGAGCA CGTACCCTGG 
GACGCGGATC CTGAGCGCGC CAAGGCCGGG GACGCGCCCC GCCGCCACAC GCACCGGCCT 
GTGGCCGAGG GCGAGCCTCG TCGCCACCGC GCGCGCCGCC GGCCCGGGGA CGAACCGGAC 
GACAGACCGG AGCGCAGGCC GCGTCCCCGC GACGCCACTA GGCCGGCCCG CGCTGCAGAC 
GGCGAAGGCG ATGATGGGGA GCGCAAGCGG CGACACCGAC ACGGGCCGCC GGCCCACGAT 
GACAGGGAGC GCAGACACCG GCGCAGAAAA GAGAGCCAGG GCTCTGGGGT CCCCATGTCT 
GGTCCCAACC TGTCCACCAC CAGGCCAATC CAGCAGGATC TGGGCCGCCA GGACCTGCCA 
CTGGCTGAGG ACCTGGACAA CATGAAGAAC AACAAGTTGG CCACCGGGGA GCCTGCCAGT 
CCCCACGACA GCCTGGGCCA CAGTGGCCTT CCCCCTAGCC CTGCCAAGAT CGGGAACAGC 
ACCAACCCTG GTCCCGCCTT GGCCACCAAT CCCCAGAATG CTGCCAGCCG CAGGACGCCC 
AACAACCCGG GCAACCCGTC CAACCCCGGC CCCCCCAAGA CTCCCGAGAA CAGCCTTATC 
GTCACCAACC CCAGCAGCAC CCAGCCCAAC TCAGCAAAGA CTGCCAGGAA ACCCGAGCAC 
ATGGCGGTGG AGATCCCCCC GGCCTGCCCG CCCCTCAACC ACACTGTGGT CCAAGTAAAC 
AAAAACGCCA ACCCAGACCC ACTGCCAAAG AAAGAGGAAG AGAAGAAGGA GGAAGAGGAG 
GCAGACCCCG GGGAGGATGG CCCAAAGCCC ATGCCGCCCT ACAGCTCCAT GTTCATCCTC 
TCCACCACCA ACCCCCTTCG CCGGCTGTGC CATTACATCC TGAACCTGCG CTACTTCGAG 
ATGTGCATCC TCATGGTCAT TGCCATGAGT AGCATCGCGC TGGCCGCCGA GGACCCGGTG 
CAGCCCAACG CACCCCGCAA CAACGTGCTG CGATATTTTG ACTATGTTTT CACAGGAGTG 
TTTACCTTTG AGATGGTGAT CAAGATGATC GACCTGGGCC TCGTCCTGCA TCAGGGGGCC 
TATTTCCGTG ACCTGTGGAA CATTCTGGAC TTCATAGTGG TCAGTGGGGC CCTGGTGGCC 
TTTGCCTTCA CGAGCAGTAC ACGTGGCAAT AGCAAAGGAA AGGACATCAA CACCATCAAG 
TCCCTCCGAG TCCTCCGGGT GCTACGACCT CTAAAGACCA TCAAGCGGCT GCCTAAGTTG 
AAGGCCGTAT TTGACTGCGT GGTGAACTCG CTCAAGAACG TCTTCAACAT CCTCATTGTC 
TACATGCTCT TCATGTTCAT CTTCGCCGTG GTGGCCGTGC AGCTCTTCAA GGGCAAATTC 
TTCCACTGCA CGGACGAGTC CAAGGAGTTT GAGAGAGACT GTCGAGGCAA ATACCTCCTT 
TACGAGAAGA ACGAGGTAAA GGCGCGGGAC CGCGAGTGGA AGAAATACGA CTTCCACTAC 
GACAACGTGC TCTGGGCCCT GCTCACGCTC TTTACGGTGT CCACGGGAGA GGGCTGGCCA 
CAGGTCCTCA AGCACTCAGT GGATGCCACT TTTGAGAACC AGGGCCCCAG CCCCGGGTAC 
CGCATGGAAA TGTCCATCTT CTACGTGGTC TACTTTGTGG TGTTTCCCTT CTTCTTTGTC 
AATATCTTTG TGGCCTTGAT CATCATCACC TTCCAGGAGC AGGGAGACAA GATGATGGAA 
GAATACAGCC TAGAGAAAAA TGAGAGGGCC TGCATCGACT TTGCCATCAG TGCCAAGCCG 
CTGACCAGGC ACATGCCCCA GAACAAGCAG AGCTTCCAGT ATCGAATGTG GCAGTTCGTG 
GTGTCCCCAC CCTTTGAGTA CACCATCATG GCCATGATCG CTCTCAACAC CATCGTGCTA 
ATGATGAAGT TCTATGGAGC CTCTGTGGCC TATGAAAACG CCCTTCGAGT GTTCAACATT 
GTCTTCACCT CCCTCTTCTC TCTCGAATGT GTGCTCAAAG TCATGGCTTT TGGGATTCTG 
AATTATTTCC GCGATGCCTG GAACATCTTC GACTTTGTGA CTGTTCTGGG CAGCATCACA 
GACATCCTCG TCACCGAGTT TGGGAATCCG AATAACTTCA TCAACCTGAG CTTTCTCCGC 
CTCTTCCGTG CTGCCCGACT CATCAAACTC CTCCGCCAGG GTTACACCAT CCGCATTCTC 
CTCTGGACTT TCGTGCAGTC TTTCAAGGCC CTACCTTATG TCTGTCTGCT GATCGCCATG 
CTCTTCTTCA TCTATGCCAT CATCGGGATG CAGGTGTTTG GCAACATCGG CATTGATGGG 
GAAGATGAGG ACAGCGATGA GGATGAGTTC CAAATCACGG AGCACAATAA CTTCCGGACC 
TTCTTCCAAG CTCTCATGCT TCTCTTCCGG AGCGCCACAG GGGAAGCGTG GCACAACATC 
ATGCTGTCCT GCCTCAGCGG GAAGCCATGC GACAAGAACT CCGGGATCCA AAAACCAGAG 
TGTGGCAACG AGTTCGCCTA TTTTTACTTT GTCTCGTTCA TCTTCCTTTG CTCATTTCTG 
ATGCTGAATC TCTTTGTTGC TGTCATCATG GACAACTTCG AGTACCTCAC CCGAGATTCC 
TCCATCCTGG GCCCCCACCA CCTGGATGAG TACGTGCGTG TCTGGGCAGA GTATGACCCT 
GCTGCCTGGG GTCGCATGCC TTACCCGGAC ATGTATCAGA TGCTGAGACA CATGTCCCCG 
CCCCTGGGTC TTGGGAAGAA GTGCCCGGCC AGAGTGGCTT ACAAGAGGCT CTTGCGGATG 
GACCTACCCG TAGCGGATGA CAACACCGTT CACTTCAACT CCACCTTGAT GGCTCTGATC 
CGAACCGCCC TGGATATCAA AATCGCAAAG GGTGGAGCTG ACAAGCAGCA AATGGACGCA 
GAGCTCCGCA AGGAAATGAT GGCCATTTGG CCCAACCTGT CTCAGAAGAC CTTGGATCTG 
CTGGTCACAC CTCACAAGTC CACGGACCTG ACAGTGGGTA AGATCTACGC AGCCATGATG 
ATCATGGAGT ACTACCGGCA GAGCAAGGCC AAGAAGCTGC AGGCCATGCG AGAGGAGCAG 
AACCGGACAC CACTCATGTT CCAGCGCATG GAGCCTCCAT CGCCAACACA GGAGGGAGGA 
CCCAGCCAAA ACGCCCTTCC CTCCACTCAG CTGGACCCCG GAGGAGGCCT GATGGCTCAA 
GAAAGCAGCA TGAAGGAGAG CCCGTCCTGG GTGACCCAGC GGGCACAGGA GATGTTCCAG 
AAGACTGGTA CCTGGAGCCC AGAGCGAGGG CCACCCATCG ACATGCCTAA CAGCCAGCCC 
AACTCCCAGT CTGTGGAGAT GAGAGAAATG GGAACTGATG GCTACTCAGA CAGCGAACAC 
TACCTCCCCA TGGAAGGACA GACCAGGGCC GCCTCCATGC CCCGCCTCCC AGCAGAGAAC 
CAGACCATCT CTGATACCAG CCCCATGAAG CGCTCAGCCT CCGTGCTGGG ACCCAAAGCC 
CGGCGACTGG ATGACTACTC ACTAGAGCGG GTACCACCTG AGGAGAACCA AAGGTACCAC 
CAACGCCGCC GGGACCGTGG CCACCGCACC TCTGAGCGCT CTCTGGGCCG ATACACTGAT 
GTGGACACAG GCCTGGGGAC AGATCTGAGC ATGACCACCC AATCGGGTGA CCTGCCCTCC 
AAAGATCGGG ACCAGGACCG GGGCCGGCCC AAGGACCGGA AGCATCGGCC ACACCACCAC 
CACCACCATC ATCACCATCA TCCCCCGGCC CCGGACCGGG AGCGCTACGC ACAGGAGCGG 
CCGGACACCG GCCGGGCGCG GGCCCGGGAG CAGCGCTGGT CCCGCTCGCC CAGCGAGGGT 
CGGGAGCACG CGACACACAG ACAGGGCAGT AGTTCCGTCA GTGGAAGCCC AGCCCCCTCT 
ACGTCCGGCA CCAGCACGCC GCGGCGCGGC CGCCGCCAGC TCCCCCAGAC CCCCTGCACC 
CCGCGGCCGC TTGTGTCCTA CTCGCCCGCT CCGCGCAGGC CTGCGGCGCG CAGGATGGCG 
GGTCCCCCAG CGCCCCCTGG CGGCTCGCCG CGGGGTTGCC GCCGTGCGCC GCGCTGGCCT 
GCGCATGCGC CTGAGGGCCC GCGGCCGCGC GGTGCCGACT ACACCGAGCC CGACAGCCCG 
CGCGAGCCGC CAGGGGGCGC GCATGAGCCC GCGCCGCGGT CGCCCAGGAC TCCGCGCGCC 
GCGGGCTGCG CGTCACCGCG CCACGGCCGC AGGCTGCCCA ATGGTTACTA CGCGGGGCAC 
GGCGCGCCAC GGCCGCGCAC AGCCCGCAGG GGCGCGCACG ACGCGTACAG CGAGAGCGAG 
GACGACTGGT GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008770581.2
CDS	106..7500
Translation	MVSSPGISERGPWMPRASWVSDPMRSPLNHELLHSSRYPGTDCTLAGSSRTLHVPWSEQMSGSLEGPTLKRRGGVVDHRDVILAHQAHKMHSTPQARRKEWEMARFGDEMPGRYGAGGGGSGPAAGVVVGAAGGRGAGGSRQGGQPGAQRMYKQSMAQRARTMALYNPIPVRQNCLTVNRSLFLFSEDNVVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDDDKTPMSERLDDTEPYFIGIFCFEAGIKIVALGFAFHKGSYLRNGWNVMDFVVVLTGILATVGTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILIFAIIGLEFYMGKFHTTCFEEGTDDIQGESPAPCGTEEPARTCPNGTKCQPYWEGPNNGITQFDNILFAVLTVFQCITMEGWTDLLYNSNDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYMEWISKAEEVILAEDETDVEQRHPFDGALRRATLKKSKTDLLNPEEAEDQLADIASVGSPFARASIKSAKLENSTFFHKKERRMRFYIRRMVKTQAFYWTVLSLVALNTLCVAIVHYNQPEWLSDFLYYAEFIFLGLFMSEMFIKMYGLGTRPYFHSSFNCFDCGVIIGSIFEVIWAVIKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFDEGTPPTNFDTFPAAIMTVFQILTGEDWNEVMYDGIKSQGGVQGGMVFSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAANQKLALQKAKEVAEVSPLSAANMSIAVKEQQKNQKPAKSVWEQRTSEMRKQNLLASREALYGDAAERWPTTYARPLRPDVKTHLDRPLVVDPQENRNNNTNKSRAPEALRQTARPRESARDPDARRAWPSSPERAPGREGPYGRESEPQQREHAPPREHVPWDADPERAKAGDAPRRHTHRPVAEGEPRRHRARRRPGDEPDDRPERRPRPRDATRPARAADGEGDDGERKRRHRHGPPAHDDRERRHRRRKESQGSGVPMSGPNLSTTRPIQQDLGRQDLPLAEDLDNMKNNKLATGEPASPHDSLGHSGLPPSPAKIGNSTNPGPALATNPQNAASRRTPNNPGNPSNPGPPKTPENSLIVTNPSSTQPNSAKTARKPEHMAVEIPPACPPLNHTVVQVNKNANPDPLPKKEEEKKEEEEADPGEDGPKPMPPYSSMFILSTTNPLRRLCHYILNLRYFEMCILMVIAMSSIALAAEDPVQPNAPRNNVLRYFDYVFTGVFTFEMVIKMIDLGLVLHQGAYFRDLWNILDFIVVSGALVAFAFTSSTRGNSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYMLFMFIFAVVAVQLFKGKFFHCTDESKEFERDCRGKYLLYEKNEVKARDREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATFENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEEYSLEKNERACIDFAISAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYTIMAMIALNTIVLMMKFYGASVAYENALRVFNIVFTSLFSLECVLKVMAFGILNYFRDAWNIFDFVTVLGSITDILVTEFGNPNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIGIDGEDEDSDEDEFQITEHNNFRTFFQALMLLFRSATGEAWHNIMLSCLSGKPCDKNSGIQKPECGNEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRVWAEYDPAAWGRMPYPDMYQMLRHMSPPLGLGKKCPARVAYKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKQQMDAELRKEMMAIWPNLSQKTLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKKLQAMREEQNRTPLMFQRMEPPSPTQEGGPSQNALPSTQLDPGGGLMAQESSMKESPSWVTQRAQEMFQKTGTWSPERGPPIDMPNSQPNSQSVEMREMGTDGYSDSEHYLPMEGQTRAASMPRLPAENQTISDTSPMKRSASVLGPKARRLDDYSLERVPPEENQRYHQRRRDRGHRTSERSLGRYTDVDTGLGTDLSMTTQSGDLPSKDRDQDRGRPKDRKHRPHHHHHHHHHHPPAPDRERYAQERPDTGRARAREQRWSRSPSEGREHATHRQGSSSVSGSPAPSTSGTSTPRRGRRQLPQTPCTPRPLVSYSPAPRRPAARRMAGPPAPPGGSPRGCRRAPRWPAHAPEGPRPRGADYTEPDSPREPPGGAHEPAPRSPRTPRAAGCASPRHGRRLPNGYYAGHGAPRPRTARRGAHDAYSESEDDWC

Target ORF information:

RefSeq Version	XM_008772359.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel subunit alpha1 A (Cacna1a), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772359.2

ORF Insert Sequence:

ATGGTTTCCT CTCCTGGGAT TTCTGAGCGA GGCCCATGGA TGCCGAGGGC CTCCTGGGTC 
TCTGACCCCA TGCGTTCTCC CCTCAATCAT GAGCTCCTGC ACAGCTCGAG GTACCCAGGC 
ACGGACTGCA CCCTGGCAGG TTCTTCCAGG ACTTTGCATG TCCCGTGGTC CGAGCAGATG 
AGCGGAAGCC TCGAGGGGCC AACTCTGAAA CGGCGTGGTG GCGTGGTGGA CCACCGGGAT 
GTCATCTTGG CCCACCAGGC GCACAAAATG CACAGCACCC CTCAGGCCAG GAGGAAAGAG 
TGGGAAATGG CCCGCTTTGG AGACGAGATG CCGGGCCGCT ACGGCGCAGG CGGAGGAGGC 
TCAGGGCCGG CCGCCGGGGT GGTCGTGGGC GCCGCGGGCG GCCGAGGAGC CGGGGGCAGC 
CGGCAGGGCG GGCAGCCCGG AGCGCAGAGG ATGTACAAGC AGTCGATGGC GCAGAGAGCG 
CGGACCATGG CCCTCTACAA CCCCATCCCT GTCCGCCAGA ACTGCCTCAC GGTCAACCGC 
TCCCTGTTCC TCTTCAGTGA AGACAACGTG GTGAGAAAAT ACGCCAAAAA GATCACGGAA 
TGGCCTCCCT TCGAGTACAT GATCCTGGCC ACCATCATTG CTAACTGCAT CGTCCTGGCC 
CTGGAGCAGC ACCTCCCTGA TGATGACAAG ACACCCATGT CCGAGCGGCT GGATGACACA 
GAACCCTATT TCATTGGCAT CTTCTGTTTT GAGGCTGGAA TTAAGATCGT GGCTCTTGGC 
TTTGCCTTCC ACAAAGGCTC CTACCTGAGG AATGGCTGGA ACGTCATGGA CTTTGTCGTG 
GTGCTAACAG GCATCTTGGC CACTGTCGGG ACGGAGTTTG ATCTACGGAC ACTGAGGGCG 
GTTCGTGTGC TGCGGCCACT CAAGCTGGTG TCTGGAATCC CAAGTTTACA AGTCGTCCTG 
AAGTCAATCA TGAAGGCGAT GATCCCTCTG CTGCAGATCG GCCTCCTCCT GTTTTTTGCA 
ATCCTTATTT TTGCAATCAT AGGGTTAGAA TTTTATATGG GAAAATTTCA TACCACCTGC 
TTTGAAGAGG GGACAGACGA CATCCAGGGT GAGTCGCCAG CTCCGTGTGG GACAGAGGAG 
CCTGCCCGCA CCTGCCCCAA CGGGACCAAA TGTCAGCCGT ACTGGGAAGG GCCCAACAAC 
GGCATCACTC AGTTCGACAA CATCCTGTTT GCTGTGCTCA CTGTTTTCCA GTGCATCACC 
ATGGAAGGCT GGACTGATCT CCTCTACAAT AGCAACGATG CCTCAGGGAA CACTTGGAAC 
TGGTTGTACT TCATCCCCCT CATCATCATC GGCTCCTTTT TTATGCTGAA CCTTGTGCTG 
GGTGTGCTGT CTGGGGAGTT TGCCAAAGAA AGGGAACGTG TAGAGAACCG AAGGGCTTTT 
CTGAAGCTCA GAAGACAACA GCAGATTGAA CGTGAGCTCA ATGGATACAT GGAGTGGATC 
TCGAAAGCAG AAGAGGTGAT CCTCGCGGAG GACGAGACAG ACGTGGAGCA GAGGCACCCT 
TTTGATGGAG CTCTTCGGAG AGCTACTCTG AAGAAAAGCA AGACGGACCT GCTCAACCCT 
GAGGAGGCGG AGGACCAGCT TGCTGACATC GCGTCTGTGG GGTCTCCCTT CGCCAGAGCC 
AGCATCAAAA GTGCCAAGCT GGAGAATTCG ACTTTTTTCC ACAAAAAGGA GAGAAGAATG 
CGTTTCTACA TCCGCCGCAT GGTCAAAACT CAGGCCTTCT ACTGGACCGT GCTCAGTCTG 
GTAGCCCTCA ACACGCTGTG CGTCGCCATT GTCCACTACA ACCAGCCCGA GTGGCTCTCC 
GACTTCCTCT ACTATGCAGA ATTCATTTTC TTAGGACTCT TTATGTCCGA AATGTTTATA 
AAAATGTATG GGCTCGGGAC ACGGCCTTAC TTCCACTCTT CCTTCAACTG CTTTGACTGT 
GGGGTCATCA TCGGGAGCAT CTTTGAAGTC ATCTGGGCCG TCATCAAACC GGGTACATCC 
TTTGGAATCA GCGTGTTACG AGCTCTCAGG TTACTGCGTA TTTTCAAAGT CACAAAGTAC 
TGGGCATCTC TCAGAAACCT GGTTGTCTCC CTCCTCAACT CCATGAAATC CATCATAAGT 
CTGCTGTTCC TCCTCTTCCT CTTCATTGTC GTCTTTGCCC TCTTGGGGAT GCAGCTGTTT 
GGTGGCCAGT TTAATTTTGA CGAGGGGACT CCTCCCACCA ACTTCGACAC TTTTCCAGCA 
GCAATAATGA CTGTGTTTCA GATCCTGACT GGCGAGGATT GGAATGAGGT CATGTATGAT 
GGGATCAAGT CTCAGGGGGG CGTGCAGGGC GGCATGGTGT TCTCCATCTA CTTCATCGTC 
CTCACCCTCT TCGGGAACTA CACCCTGCTG AACGTGTTCT TGGCTATCGC GGTGGACAAC 
CTGGCCAACG CCCAGGAACT CACCAAGGAT GAACAAGAAG AGGAAGAGGC AGCCAATCAG 
AAACTGGCTC TACAGAAAGC CAAGGAGGTG GCAGAAGTGA GTCCCCTGTC TGCAGCCAAC 
ATGTCCATAG CTGTGAAGGA ACAGCAGAAG AACCAGAAGC CTGCCAAGTC GGTGTGGGAG 
CAGCGCACCA GCGAGATGCG CAAGCAGAAC CTGCTGGCTA GCCGCGAGGC GCTGTACGGG 
GACGCGGCTG AGCGCTGGCC CACCACTTAC GCGCGCCCGC TGCGGCCGGA CGTGAAGACG 
CACTTGGACC GGCCGCTCGT GGTGGACCCG CAGGAGAACC GTAACAACAA CACCAACAAG 
AGCCGTGCGC CAGAAGCGCT GCGCCAAACC GCGCGGCCCC GCGAGAGCGC GCGCGACCCC 
GACGCGCGGC GCGCCTGGCC CAGCAGCCCT GAGCGCGCCC CTGGACGAGA GGGCCCGTAT 
GGCCGCGAGA GCGAGCCGCA ACAGCGCGAG CACGCGCCAC CCCGCGAGCA CGTACCCTGG 
GACGCGGATC CTGAGCGCGC CAAGGCCGGG GACGCGCCCC GCCGCCACAC GCACCGGCCT 
GTGGCCGAGG GCGAGCCTCG TCGCCACCGC GCGCGCCGCC GGCCCGGGGA CGAACCGGAC 
GACAGACCGG AGCGCAGGCC GCGTCCCCGC GACGCCACTA GGCCGGCCCG CGCTGCAGAC 
GGCGAAGGCG ATGATGGGGA GCGCAAGCGG CGACACCGAC ACGGGCCGCC GGCCCACGAT 
GACAGGGAGC GCAGACACCG GCGCAGAAAA GAGAGCCAGG GCTCTGGGGT CCCCATGTCT 
GGTCCCAACC TGTCCACCAC CAGGCCAATC CAGCAGGATC TGGGCCGCCA GGACCTGCCA 
CTGGCTGAGG ACCTGGACAA CATGAAGAAC AACAAGTTGG CCACCGGGGA GCCTGCCAGT 
CCCCACGACA GCCTGGGCCA CAGTGGCCTT CCCCCTAGCC CTGCCAAGAT CGGGAACAGC 
ACCAACCCTG GTCCCGCCTT GGCCACCAAT CCCCAGAATG CTGCCAGCCG CAGGACGCCC 
AACAACCCGG GCAACCCGTC CAACCCCGGC CCCCCCAAGA CTCCCGAGAA CAGCCTTATC 
GTCACCAACC CCAGCAGCAC CCAGCCCAAC TCAGCAAAGA CTGCCAGGAA ACCCGAGCAC 
ATGGCGGTGG AGATCCCCCC GGCCTGCCCG CCCCTCAACC ACACTGTGGT CCAAGTAAAC 
AAAAACGCCA ACCCAGACCC ACTGCCAAAG AAAGAGGAAG AGAAGAAGGA GGAAGAGGAG 
GCAGACCCCG GGGAGGATGG CCCAAAGCCC ATGCCGCCCT ACAGCTCCAT GTTCATCCTC 
TCCACCACCA ACCCCCTTCG CCGGCTGTGC CATTACATCC TGAACCTGCG CTACTTCGAG 
ATGTGCATCC TCATGGTCAT TGCCATGAGT AGCATCGCGC TGGCCGCCGA GGACCCGGTG 
CAGCCCAACG CACCCCGCAA CAACGTGCTG CGATATTTTG ACTATGTTTT CACAGGAGTG 
TTTACCTTTG AGATGGTGAT CAAGATGATC GACCTGGGCC TCGTCCTGCA TCAGGGGGCC 
TATTTCCGTG ACCTGTGGAA CATTCTGGAC TTCATAGTGG TCAGTGGGGC CCTGGTGGCC 
TTTGCCTTCA CGAGCAGTAC ACGTGGCAAT AGCAAAGGAA AGGACATCAA CACCATCAAG 
TCCCTCCGAG TCCTCCGGGT GCTACGACCT CTAAAGACCA TCAAGCGGCT GCCTAAGTTG 
AAGGCCGTAT TTGACTGCGT GGTGAACTCG CTCAAGAACG TCTTCAACAT CCTCATTGTC 
TACATGCTCT TCATGTTCAT CTTCGCCGTG GTGGCCGTGC AGCTCTTCAA GGGCAAATTC 
TTCCACTGCA CGGACGAGTC CAAGGAGTTT GAGAGAGACT GTCGAGGCAA ATACCTCCTT 
TACGAGAAGA ACGAGGTAAA GGCGCGGGAC CGCGAGTGGA AGAAATACGA CTTCCACTAC 
GACAACGTGC TCTGGGCCCT GCTCACGCTC TTTACGGTGT CCACGGGAGA GGGCTGGCCA 
CAGGTCCTCA AGCACTCAGT GGATGCCACT TTTGAGAACC AGGGCCCCAG CCCCGGGTAC 
CGCATGGAAA TGTCCATCTT CTACGTGGTC TACTTTGTGG TGTTTCCCTT CTTCTTTGTC 
AATATCTTTG TGGCCTTGAT CATCATCACC TTCCAGGAGC AGGGAGACAA GATGATGGAA 
GAATACAGCC TAGAGAAAAA TGAGAGGGCC TGCATCGACT TTGCCATCAG TGCCAAGCCG 
CTGACCAGGC ACATGCCCCA GAACAAGCAG AGCTTCCAGT ATCGAATGTG GCAGTTCGTG 
GTGTCCCCAC CCTTTGAGTA CACCATCATG GCCATGATCG CTCTCAACAC CATCGTGCTA 
ATGATGAAGT TCTATGGAGC CTCTGTGGCC TATGAAAACG CCCTTCGAGT GTTCAACATT 
GTCTTCACCT CCCTCTTCTC TCTCGAATGT GTGCTCAAAG TCATGGCTTT TGGGATTCTG 
AATTATTTCC GCGATGCCTG GAACATCTTC GACTTTGTGA CTGTTCTGGG CAGCATCACA 
GACATCCTCG TCACCGAGTT TGGGAATCCG AATAACTTCA TCAACCTGAG CTTTCTCCGC 
CTCTTCCGTG CTGCCCGACT CATCAAACTC CTCCGCCAGG GTTACACCAT CCGCATTCTC 
CTCTGGACTT TCGTGCAGTC TTTCAAGGCC CTACCTTATG TCTGTCTGCT GATCGCCATG 
CTCTTCTTCA TCTATGCCAT CATCGGGATG CAGGTGTTTG GCAACATCGG CATTGATGGG 
GAAGATGAGG ACAGCGATGA GGATGAGTTC CAAATCACGG AGCACAATAA CTTCCGGACC 
TTCTTCCAAG CTCTCATGCT TCTCTTCCGG AGCGCCACAG GGGAAGCGTG GCACAACATC 
ATGCTGTCCT GCCTCAGCGG GAAGCCATGC GACAAGAACT CCGGGATCCA AAAACCAGAG 
TGTGGCAACG AGTTCGCCTA TTTTTACTTT GTCTCGTTCA TCTTCCTTTG CTCATTTCTG 
ATGCTGAATC TCTTTGTTGC TGTCATCATG GACAACTTCG AGTACCTCAC CCGAGATTCC 
TCCATCCTGG GCCCCCACCA CCTGGATGAG TACGTGCGTG TCTGGGCAGA GTATGACCCT 
GCTGCCTGGG GTCGCATGCC TTACCCGGAC ATGTATCAGA TGCTGAGACA CATGTCCCCG 
CCCCTGGGTC TTGGGAAGAA GTGCCCGGCC AGAGTGGCTT ACAAGAGGCT CTTGCGGATG 
GACCTACCCG TAGCGGATGA CAACACCGTT CACTTCAACT CCACCTTGAT GGCTCTGATC 
CGAACCGCCC TGGATATCAA AATCGCAAAG GGTGGAGCTG ACAAGCAGCA AATGGACGCA 
GAGCTCCGCA AGGAAATGAT GGCCATTTGG CCCAACCTGT CTCAGAAGAC CTTGGATCTG 
CTGGTCACAC CTCACAAGTC CACGGACCTG ACAGTGGGTA AGATCTACGC AGCCATGATG 
ATCATGGAGT ACTACCGGCA GAGCAAGGCC AAGAAGCTGC AGGCCATGCG AGAGGAGCAG 
AACCGGACAC CACTCATGTT CCAGCGCATG GAGCCTCCAT CGCCAACACA GGAGGGAGGA 
CCCAGCCAAA ACGCCCTTCC CTCCACTCAG CTGGACCCCG GAGGAGGCCT GATGGCTCAA 
GAAAGCAGCA TGAAGGAGAG CCCGTCCTGG GTGACCCAGC GGGCACAGGA GATGTTCCAG 
AAGACTGGTA CCTGGAGCCC AGAGCGAGGG CCACCCATCG ACATGCCTAA CAGCCAGCCC 
AACTCCCAGT CTGTGGAGAT GAGAGAAATG GGAACTGATG GCTACTCAGA CAGCGAACAC 
TACCTCCCCA TGGAAGGACA GACCAGGGCC GCCTCCATGC CCCGCCTCCC AGCAGAGAAC 
CAGACCATCT CTGATACCAG CCCCATGAAG CGCTCAGCCT CCGTGCTGGG ACCCAAAGCC 
CGGCGACTGG ATGACTACTC ACTAGAGCGG GTACCACCTG AGGAGAACCA AAGGTACCAC 
CAACGCCGCC GGGACCGTGG CCACCGCACC TCTGAGCGCT CTCTGGGCCG ATACACTGAT 
GTGGACACAG GCCTGGGGAC AGATCTGAGC ATGACCACCC AATCGGGTGA CCTGCCCTCC 
AAAGATCGGG ACCAGGACCG GGGCCGGCCC AAGGACCGGA AGCATCGGCC ACACCACCAC 
CACCACCATC ATCACCATCA TCCCCCGGCC CCGGACCGGG AGCGCTACGC ACAGGAGCGG 
CCGGACACCG GCCGGGCGCG GGCCCGGGAG CAGCGCTGGT CCCGCTCGCC CAGCGAGGGT 
CGGGAGCACG CGACACACAG ACAGGGCAGT AGTTCCGTCA GTGGAAGCCC AGCCCCCTCT 
ACGTCCGGCA CCAGCACGCC GCGGCGCGGC CGCCGCCAGC TCCCCCAGAC CCCCTGCACC 
CCGCGGCCGC TTGTGTCCTA CTCGCCCGCT CCGCGCAGGC CTGCGGCGCG CAGGATGGCG 
GGTCCCCCAG CGCCCCCTGG CGGCTCGCCG CGGGGTTGCC GCCGTGCGCC GCGCTGGCCT 
GCGCATGCGC CTGAGGGCCC GCGGCCGCGC GGTGCCGACT ACACCGAGCC CGACAGCCCG 
CGCGAGCCGC CAGGGGGCGC GCATGAGCCC GCGCCGCGGT CGCCCAGGAC TCCGCGCGCC 
GCGGGCTGCG CGTCACCGCG CCACGGCCGC AGGCTGCCCA ATGGTTACTA CGCGGGGCAC 
GGCGCGCCAC GGCCGCGCAC AGCCCGCAGG GGCGCGCACG ACGCGTACAG CGAGAGCGAG 
GACGACTGGT GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1a ( XM_008772360.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008770582.2 Rattus norvegicus calcium voltage-gated channel subunit alpha1 A (Cacna1a), transcript variant X16, mRNA.

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CloneID	ORa59379
Clone ID Related Accession (Same CDS sequence)	XM_008772360.2
Accession Version	XM_008772360.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008772360.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTCCT CTCCTGGGAT TTCTGAGCGA GGCCCATGGA TGCCGAGGGC CTCCTGGGTC 
TCTGACCCCA TGCGTTCTCC CCTCAATCAT GAGCTCCTGC ACAGCTCGAG GTACCCAGGC 
ACGGACTGCA CCCTGGCAGG TTCTTCCAGG ACTTTGCATG TCCCGTGGTC CGAGCAGATG 
AGCGGAAGCC TCGAGGGGCC AACTCTGAAA CGGCGTGGTG GCGTGGTGGA CCACCGGGAT 
GTCATCTTGG CCCACCAGGC GCACAAAATG CACAGCACCC CTCAGGCCAG GAGGAAAGAG 
TGGGAAATGG CCCGCTTTGG AGACGAGATG CCGGGCCGCT ACGGCGCAGG CGGAGGAGGC 
TCAGGGCCGG CCGCCGGGGT GGTCGTGGGC GCCGCGGGCG GCCGAGGAGC CGGGGGCAGC 
CGGCAGGGCG GGCAGCCCGG AGCGCAGAGG ATGTACAAGC AGTCGATGGC GCAGAGAGCG 
CGGACCATGG CCCTCTACAA CCCCATCCCT GTCCGCCAGA ACTGCCTCAC GGTCAACCGC 
TCCCTGTTCC TCTTCAGTGA AGACAACGTG GTGAGAAAAT ACGCCAAAAA GATCACGGAA 
TGGCCTCCCT TCGAGTACAT GATCCTGGCC ACCATCATTG CTAACTGCAT CGTCCTGGCC 
CTGGAGCAGC ACCTCCCTGA TGATGACAAG ACACCCATGT CCGAGCGGCT GGATGACACA 
GAACCCTATT TCATTGGCAT CTTCTGTTTT GAGGCTGGAA TTAAGATCGT GGCTCTTGGC 
TTTGCCTTCC ACAAAGGCTC CTACCTGAGG AATGGCTGGA ACGTCATGGA CTTTGTCGTG 
GTGCTAACAG GCATCTTGGC CACTGTCGGG ACGGAGTTTG ATCTACGGAC ACTGAGGGCG 
GTTCGTGTGC TGCGGCCACT CAAGCTGGTG TCTGGAATCC CAAGTTTACA AGTCGTCCTG 
AAGTCAATCA TGAAGGCGAT GATCCCTCTG CTGCAGATCG GCCTCCTCCT GTTTTTTGCA 
ATCCTTATTT TTGCAATCAT AGGGTTAGAA TTTTATATGG GAAAATTTCA TACCACCTGC 
TTTGAAGAGG GGACAGACGA CATCCAGGGT GAGTCGCCAG CTCCGTGTGG GACAGAGGAG 
CCTGCCCGCA CCTGCCCCAA CGGGACCAAA TGTCAGCCGT ACTGGGAAGG GCCCAACAAC 
GGCATCACTC AGTTCGACAA CATCCTGTTT GCTGTGCTCA CTGTTTTCCA GTGCATCACC 
ATGGAAGGCT GGACTGATCT CCTCTACAAT AGCAACGATG CCTCAGGGAA CACTTGGAAC 
TGGTTGTACT TCATCCCCCT CATCATCATC GGCTCCTTTT TTATGCTGAA CCTTGTGCTG 
GGTGTGCTGT CTGGGGAGTT TGCCAAAGAA AGGGAACGTG TAGAGAACCG AAGGGCTTTT 
CTGAAGCTCA GAAGACAACA GCAGATTGAA CGTGAGCTCA ATGGATACAT GGAGTGGATC 
TCGAAAGCAG AAGAGGTGAT CCTCGCGGAG GACGAGACAG ACGTGGAGCA GAGGCACCCT 
TTTGATGGAG CTCTTCGGAG AGCTACTCTG AAGAAAAGCA AGACGGACCT GCTCAACCCT 
GAGGAGGCGG AGGACCAGCT TGCTGACATC GCGTCTGTGG GGTCTCCCTT CGCCAGAGCC 
AGCATCAAAA GTGCCAAGCT GGAGAATTCG ACTTTTTTCC ACAAAAAGGA GAGAAGAATG 
CGTTTCTACA TCCGCCGCAT GGTCAAAACT CAGGCCTTCT ACTGGACCGT GCTCAGTCTG 
GTAGCCCTCA ACACGCTGTG CGTCGCCATT GTCCACTACA ACCAGCCCGA GTGGCTCTCC 
GACTTCCTCT ACTATGCAGA ATTCATTTTC TTAGGACTCT TTATGTCCGA AATGTTTATA 
AAAATGTATG GGCTCGGGAC ACGGCCTTAC TTCCACTCTT CCTTCAACTG CTTTGACTGT 
GGGGTCATCA TCGGGAGCAT CTTTGAAGTC ATCTGGGCCG TCATCAAACC GGGTACATCC 
TTTGGAATCA GCGTGTTACG AGCTCTCAGG TTACTGCGTA TTTTCAAAGT CACAAAGTAC 
TGGGCATCTC TCAGAAACCT GGTTGTCTCC CTCCTCAACT CCATGAAATC CATCATAAGT 
CTGCTGTTCC TCCTCTTCCT CTTCATTGTC GTCTTTGCCC TCTTGGGGAT GCAGCTGTTT 
GGTGGCCAGT TTAATTTTGA CGAGGGGACT CCTCCCACCA ACTTCGACAC TTTTCCAGCA 
GCAATAATGA CTGTGTTTCA GATCCTGACT GGCGAGGATT GGAATGAGGT CATGTATGAT 
GGGATCAAGT CTCAGGGGGG CGTGCAGGGC GGCATGGTGT TCTCCATCTA CTTCATCGTC 
CTCACCCTCT TCGGGAACTA CACCCTGCTG AACGTGTTCT TGGCTATCGC GGTGGACAAC 
CTGGCCAACG CCCAGGAACT CACCAAGGAT GAACAAGAAG AGGAAGAGGC AGCCAATCAG 
AAACTGGCTC TACAGAAAGC CAAGGAGGTG GCAGAAGTGA GTCCCCTGTC TGCAGCCAAC 
ATGTCCATAG CTGTGAAGGA ACAGCAGAAG AACCAGAAGC CTGCCAAGTC GGTGTGGGAG 
CAGCGCACCA GCGAGATGCG CAAGCAGAAC CTGCTGGCTA GCCGCGAGGC GCTGTACGGG 
GACGCGGCTG AGCGCTGGCC CACCACTTAC GCGCGCCCGC TGCGGCCGGA CGTGAAGACG 
CACTTGGACC GGCCGCTCGT GGTGGACCCG CAGGAGAACC GTAACAACAA CACCAACAAG 
AGCCGTGCGC CAGAAGCGCT GCGCCAAACC GCGCGGCCCC GCGAGAGCGC GCGCGACCCC 
GACGCGCGGC GCGCCTGGCC CAGCAGCCCT GAGCGCGCCC CTGGACGAGA GGGCCCGTAT 
GGCCGCGAGA GCGAGCCGCA ACAGCGCGAG CACGCGCCAC CCCGCGAGCA CGTACCCTGG 
GACGCGGATC CTGAGCGCGC CAAGGCCGGG GACGCGCCCC GCCGCCACAC GCACCGGCCT 
GTGGCCGAGG GCGAGCCTCG TCGCCACCGC GCGCGCCGCC GGCCCGGGGA CGAACCGGAC 
GACAGACCGG AGCGCAGGCC GCGTCCCCGC GACGCCACTA GGCCGGCCCG CGCTGCAGAC 
GGCGAAGGCG ATGATGGGGA GCGCAAGCGG CGACACCGAC ACGGGCCGCC GGCCCACGAT 
GACAGGGAGC GCAGACACCG GCGCAGAAAA GAGAGCCAGG GCTCTGGGGT CCCCATGTCT 
GGTCCCAACC TGTCCACCAC CAGGCCAATC CAGCAGGATC TGGGCCGCCA GGACCTGCCA 
CTGGCTGAGG ACCTGGACAA CATGAAGAAC AACAAGTTGG CCACCGGGGA GCCTGCCAGT 
CCCCACGACA GCCTGGGCCA CAGTGGCCTT CCCCCTAGCC CTGCCAAGAT CGGGAACAGC 
ACCAACCCTG GTCCCGCCTT GGCCACCAAT CCCCAGAATG CTGCCAGCCG CAGGACGCCC 
AACAACCCGG GCAACCCGTC CAACCCCGGC CCCCCCAAGA CTCCCGAGAA CAGCCTTATC 
GTCACCAACC CCAGCAGCAC CCAGCCCAAC TCAGCAAAGA CTGCCAGGAA ACCCGAGCAC 
ATGGCGGTGG AGATCCCCCC GGCCTGCCCG CCCCTCAACC ACACTGTGGT CCAAGTAAAC 
AAAAACGCCA ACCCAGACCC ACTGCCAAAG AAAGAGGAAG AGAAGAAGGA GGAAGAGGAG 
GCAGACCCCG GGGAGGATGG CCCAAAGCCC ATGCCGCCCT ACAGCTCCAT GTTCATCCTC 
TCCACCACCA ACCCCCTTCG CCGGCTGTGC CATTACATCC TGAACCTGCG CTACTTCGAG 
ATGTGCATCC TCATGGTCAT TGCCATGAGT AGCATCGCGC TGGCCGCCGA GGACCCGGTG 
CAGCCCAACG CACCCCGCAA CAACGTGCTG CGATATTTTG ACTATGTTTT CACAGGAGTG 
TTTACCTTTG AGATGGTGAT CAAGATGATC GACCTGGGCC TCGTCCTGCA TCAGGGGGCC 
TATTTCCGTG ACCTGTGGAA CATTCTGGAC TTCATAGTGG TCAGTGGGGC CCTGGTGGCC 
TTTGCCTTCA CGAGCAGTAC ACGTGGCAAT AGCAAAGGAA AGGACATCAA CACCATCAAG 
TCCCTCCGAG TCCTCCGGGT GCTACGACCT CTAAAGACCA TCAAGCGGCT GCCTAAGTTG 
AAGGCCGTAT TTGACTGCGT GGTGAACTCG CTCAAGAACG TCTTCAACAT CCTCATTGTC 
TACATGCTCT TCATGTTCAT CTTCGCCGTG GTGGCCGTGC AGCTCTTCAA GGGCAAATTC 
TTCCACTGCA CGGACGAGTC CAAGGAGTTT GAGAGAGACT GTCGAGGCAA ATACCTCCTT 
TACGAGAAGA ACGAGGTAAA GGCGCGGGAC CGCGAGTGGA AGAAATACGA CTTCCACTAC 
GACAACGTGC TCTGGGCCCT GCTCACGCTC TTTACGGTGT CCACGGGAGA GGGCTGGCCA 
CAGGTCCTCA AGCACTCAGT GGATGCCACT TTTGAGAACC AGGGCCCCAG CCCCGGGTAC 
CGCATGGAAA TGTCCATCTT CTACGTGGTC TACTTTGTGG TGTTTCCCTT CTTCTTTGTC 
AATATCTTTG TGGCCTTGAT CATCATCACC TTCCAGGAGC AGGGAGACAA GATGATGGAA 
GAATACAGCC TAGAGAAAAA TGAGAGGGCC TGCATCGACT TTGCCATCAG TGCCAAGCCG 
CTGACCAGGC ACATGCCCCA GAACAAGCAG AGCTTCCAGT ATCGAATGTG GCAGTTCGTG 
GTGTCCCCAC CCTTTGAGTA CACCATCATG GCCATGATCG CTCTCAACAC CATCGTGCTA 
ATGATGAAGT TCTATGGAGC CTCTGTGGCC TATGAAAACG CCCTTCGAGT GTTCAACATT 
GTCTTCACCT CCCTCTTCTC TCTCGAATGT GTGCTCAAAG TCATGGCTTT TGGGATTCTG 
AATTATTTCC GCGATGCCTG GAACATCTTC GACTTTGTGA CTGTTCTGGG CAGCATCACA 
GACATCCTCG TCACCGAGTT TGGGAATCCG AATAACTTCA TCAACCTGAG CTTTCTCCGC 
CTCTTCCGTG CTGCCCGACT CATCAAACTC CTCCGCCAGG GTTACACCAT CCGCATTCTC 
CTCTGGACTT TCGTGCAGTC TTTCAAGGCC CTACCTTATG TCTGTCTGCT GATCGCCATG 
CTCTTCTTCA TCTATGCCAT CATCGGGATG CAGGTGATCC CGCACCCTTG GGGTACCGGG 
GCTGCGGGAG GGAGCTTGTG TCAAAGGGAG CCAAGGTGGC TGTGGCTGCT CCCATCGTCA 
CCCCCCCCCC CAACTCTCCA GGTGTTTGGC AACATCGGCA TTGATGGGGA AGATGAGGAC 
AGCGATGAGG ATGAGTTCCA AATCACGGAG CACAATAACT TCCGGACCTT CTTCCAAGCT 
CTCATGCTTC TCTTCCGGAG CGCCACAGGG GAAGCGTGGC ACAACATCAT GCTGTCCTGC 
CTCAGCGGGA AGCCATGCGA CAAGAACTCC GGGATCCAAA AACCAGAGTG TGGCAACGAG 
TTCGCCTATT TTTACTTTGT CTCGTTCATC TTCCTTTGCT CATTTCTGAT GCTGAATCTC 
TTTGTTGCTG TCATCATGGA CAACTTCGAG TACCTCACCC GAGATTCCTC CATCCTGGGC 
CCCCACCACC TGGATGAGTA CGTGCGTGTC TGGGCAGAGT ATGACCCTGC TGCCTGCGGC 
CGCATTCACT ATAAGGACAT GTACAGTTTA TTGCGAGTAA TATCGCCCCC TCTCGGCTTA 
GGCAAGAAAT GTCCTCATAG GGTTGCTTGC AAGAGGCTCT TGCGGATGGA CCTACCCGTA 
GCGGATGACA ACACCGTTCA CTTCAACTCC ACCTTGATGG CTCTGATCCG AACCGCCCTG 
GATATCAAAA TCGCAAAGGG TGGAGCTGAC AAGCAGCAAA TGGACGCAGA GCTCCGCAAG 
GAAATGATGG CCATTTGGCC CAACCTGTCT CAGAAGACCT TGGATCTGCT GGTCACACCT 
CACAAGTCCA CGGACCTGAC AGTGGGTAAG ATCTACGCAG CCATGATGAT CATGGAGTAC 
TACCGGCAGA GCAAGGCCAA GAAGCTGCAG GCCATGCGAG AGGAGCAGAA CCGGACACCA 
CTCATGTTCC AGCGCATGGA GCCTCCATCG CCAACACAGG AGGGAGGACC CAGCCAAAAC 
GCCCTTCCCT CCACTCAGCT GGACCCCGGA GGAGGCCTGA TGGCTCAAGA AAGCAGCATG 
AAGGAGAGCC CGTCCTGGGT GACCCAGCGG GCACAGGAGA TGTTCCAGAA GACTGGTACC 
TGGAGCCCAG AGCGAGGGCC ACCCATCGAC ATGCCTAACA GCCAGCCCAA CTCCCAGTCT 
GTGGAGATGA GAGAAATGGG AACTGATGGC TACTCAGACA GCGAACACTA CCTCCCCATG 
GAAGGACAGA CCAGGGCCGC CTCCATGCCC CGCCTCCCAG CAGAGAACCA GAGGAGAAGG 
GGCCGGCCAC GTGGAAATAA CCTCAGTACC ATCTCTGATA CCAGCCCCAT GAAGCGCTCA 
GCCTCCGTGC TGGGACCCAA AGCCCGGCGA CTGGATGACT ACTCACTAGA GCGGGTACCA 
CCTGAGGAGA ACCAAAGGTA CCACCAACGC CGCCGGGACC GTGGCCACCG CACCTCTGAG 
CGCTCTCTGG GCCGATACAC TGATGTGGAC ACAGGCCTGG GGACAGATCT GAGCATGACC 
ACCCAATCGG GTGACCTGCC CTCCAAAGAT CGGGACCAGG ACCGGGGCCG GCCCAAGGAC 
CGGAAGCATC GGCCACACCA CCACCACCAC CATCATCACC ATCATCCCCC GGCCCCGGAC 
CGGGAGCGCT ACGCACAGGA GCGGCCGGAC ACCGGCCGGG CGCGGGCCCG GGAGCAGCGC 
TGGTCCCGCT CGCCCAGCGA GGGTCGGGAG CACGCGACAC ACAGACAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008770582.2
CDS	105..7175
Translation	MVSSPGISERGPWMPRASWVSDPMRSPLNHELLHSSRYPGTDCTLAGSSRTLHVPWSEQMSGSLEGPTLKRRGGVVDHRDVILAHQAHKMHSTPQARRKEWEMARFGDEMPGRYGAGGGGSGPAAGVVVGAAGGRGAGGSRQGGQPGAQRMYKQSMAQRARTMALYNPIPVRQNCLTVNRSLFLFSEDNVVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDDDKTPMSERLDDTEPYFIGIFCFEAGIKIVALGFAFHKGSYLRNGWNVMDFVVVLTGILATVGTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILIFAIIGLEFYMGKFHTTCFEEGTDDIQGESPAPCGTEEPARTCPNGTKCQPYWEGPNNGITQFDNILFAVLTVFQCITMEGWTDLLYNSNDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYMEWISKAEEVILAEDETDVEQRHPFDGALRRATLKKSKTDLLNPEEAEDQLADIASVGSPFARASIKSAKLENSTFFHKKERRMRFYIRRMVKTQAFYWTVLSLVALNTLCVAIVHYNQPEWLSDFLYYAEFIFLGLFMSEMFIKMYGLGTRPYFHSSFNCFDCGVIIGSIFEVIWAVIKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFDEGTPPTNFDTFPAAIMTVFQILTGEDWNEVMYDGIKSQGGVQGGMVFSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAANQKLALQKAKEVAEVSPLSAANMSIAVKEQQKNQKPAKSVWEQRTSEMRKQNLLASREALYGDAAERWPTTYARPLRPDVKTHLDRPLVVDPQENRNNNTNKSRAPEALRQTARPRESARDPDARRAWPSSPERAPGREGPYGRESEPQQREHAPPREHVPWDADPERAKAGDAPRRHTHRPVAEGEPRRHRARRRPGDEPDDRPERRPRPRDATRPARAADGEGDDGERKRRHRHGPPAHDDRERRHRRRKESQGSGVPMSGPNLSTTRPIQQDLGRQDLPLAEDLDNMKNNKLATGEPASPHDSLGHSGLPPSPAKIGNSTNPGPALATNPQNAASRRTPNNPGNPSNPGPPKTPENSLIVTNPSSTQPNSAKTARKPEHMAVEIPPACPPLNHTVVQVNKNANPDPLPKKEEEKKEEEEADPGEDGPKPMPPYSSMFILSTTNPLRRLCHYILNLRYFEMCILMVIAMSSIALAAEDPVQPNAPRNNVLRYFDYVFTGVFTFEMVIKMIDLGLVLHQGAYFRDLWNILDFIVVSGALVAFAFTSSTRGNSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYMLFMFIFAVVAVQLFKGKFFHCTDESKEFERDCRGKYLLYEKNEVKARDREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATFENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEEYSLEKNERACIDFAISAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYTIMAMIALNTIVLMMKFYGASVAYENALRVFNIVFTSLFSLECVLKVMAFGILNYFRDAWNIFDFVTVLGSITDILVTEFGNPNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVIPHPWGTGAAGGSLCQREPRWLWLLPSSPPPPTLQVFGNIGIDGEDEDSDEDEFQITEHNNFRTFFQALMLLFRSATGEAWHNIMLSCLSGKPCDKNSGIQKPECGNEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRVWAEYDPAACGRIHYKDMYSLLRVISPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKQQMDAELRKEMMAIWPNLSQKTLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKKLQAMREEQNRTPLMFQRMEPPSPTQEGGPSQNALPSTQLDPGGGLMAQESSMKESPSWVTQRAQEMFQKTGTWSPERGPPIDMPNSQPNSQSVEMREMGTDGYSDSEHYLPMEGQTRAASMPRLPAENQRRRGRPRGNNLSTISDTSPMKRSASVLGPKARRLDDYSLERVPPEENQRYHQRRRDRGHRTSERSLGRYTDVDTGLGTDLSMTTQSGDLPSKDRDQDRGRPKDRKHRPHHHHHHHHHHPPAPDRERYAQERPDTGRARAREQRWSRSPSEGREHATHRQ

Target ORF information:

RefSeq Version	XM_008772360.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel subunit alpha1 A (Cacna1a), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772360.2

ORF Insert Sequence:

ATGGTTTCCT CTCCTGGGAT TTCTGAGCGA GGCCCATGGA TGCCGAGGGC CTCCTGGGTC 
TCTGACCCCA TGCGTTCTCC CCTCAATCAT GAGCTCCTGC ACAGCTCGAG GTACCCAGGC 
ACGGACTGCA CCCTGGCAGG TTCTTCCAGG ACTTTGCATG TCCCGTGGTC CGAGCAGATG 
AGCGGAAGCC TCGAGGGGCC AACTCTGAAA CGGCGTGGTG GCGTGGTGGA CCACCGGGAT 
GTCATCTTGG CCCACCAGGC GCACAAAATG CACAGCACCC CTCAGGCCAG GAGGAAAGAG 
TGGGAAATGG CCCGCTTTGG AGACGAGATG CCGGGCCGCT ACGGCGCAGG CGGAGGAGGC 
TCAGGGCCGG CCGCCGGGGT GGTCGTGGGC GCCGCGGGCG GCCGAGGAGC CGGGGGCAGC 
CGGCAGGGCG GGCAGCCCGG AGCGCAGAGG ATGTACAAGC AGTCGATGGC GCAGAGAGCG 
CGGACCATGG CCCTCTACAA CCCCATCCCT GTCCGCCAGA ACTGCCTCAC GGTCAACCGC 
TCCCTGTTCC TCTTCAGTGA AGACAACGTG GTGAGAAAAT ACGCCAAAAA GATCACGGAA 
TGGCCTCCCT TCGAGTACAT GATCCTGGCC ACCATCATTG CTAACTGCAT CGTCCTGGCC 
CTGGAGCAGC ACCTCCCTGA TGATGACAAG ACACCCATGT CCGAGCGGCT GGATGACACA 
GAACCCTATT TCATTGGCAT CTTCTGTTTT GAGGCTGGAA TTAAGATCGT GGCTCTTGGC 
TTTGCCTTCC ACAAAGGCTC CTACCTGAGG AATGGCTGGA ACGTCATGGA CTTTGTCGTG 
GTGCTAACAG GCATCTTGGC CACTGTCGGG ACGGAGTTTG ATCTACGGAC ACTGAGGGCG 
GTTCGTGTGC TGCGGCCACT CAAGCTGGTG TCTGGAATCC CAAGTTTACA AGTCGTCCTG 
AAGTCAATCA TGAAGGCGAT GATCCCTCTG CTGCAGATCG GCCTCCTCCT GTTTTTTGCA 
ATCCTTATTT TTGCAATCAT AGGGTTAGAA TTTTATATGG GAAAATTTCA TACCACCTGC 
TTTGAAGAGG GGACAGACGA CATCCAGGGT GAGTCGCCAG CTCCGTGTGG GACAGAGGAG 
CCTGCCCGCA CCTGCCCCAA CGGGACCAAA TGTCAGCCGT ACTGGGAAGG GCCCAACAAC 
GGCATCACTC AGTTCGACAA CATCCTGTTT GCTGTGCTCA CTGTTTTCCA GTGCATCACC 
ATGGAAGGCT GGACTGATCT CCTCTACAAT AGCAACGATG CCTCAGGGAA CACTTGGAAC 
TGGTTGTACT TCATCCCCCT CATCATCATC GGCTCCTTTT TTATGCTGAA CCTTGTGCTG 
GGTGTGCTGT CTGGGGAGTT TGCCAAAGAA AGGGAACGTG TAGAGAACCG AAGGGCTTTT 
CTGAAGCTCA GAAGACAACA GCAGATTGAA CGTGAGCTCA ATGGATACAT GGAGTGGATC 
TCGAAAGCAG AAGAGGTGAT CCTCGCGGAG GACGAGACAG ACGTGGAGCA GAGGCACCCT 
TTTGATGGAG CTCTTCGGAG AGCTACTCTG AAGAAAAGCA AGACGGACCT GCTCAACCCT 
GAGGAGGCGG AGGACCAGCT TGCTGACATC GCGTCTGTGG GGTCTCCCTT CGCCAGAGCC 
AGCATCAAAA GTGCCAAGCT GGAGAATTCG ACTTTTTTCC ACAAAAAGGA GAGAAGAATG 
CGTTTCTACA TCCGCCGCAT GGTCAAAACT CAGGCCTTCT ACTGGACCGT GCTCAGTCTG 
GTAGCCCTCA ACACGCTGTG CGTCGCCATT GTCCACTACA ACCAGCCCGA GTGGCTCTCC 
GACTTCCTCT ACTATGCAGA ATTCATTTTC TTAGGACTCT TTATGTCCGA AATGTTTATA 
AAAATGTATG GGCTCGGGAC ACGGCCTTAC TTCCACTCTT CCTTCAACTG CTTTGACTGT 
GGGGTCATCA TCGGGAGCAT CTTTGAAGTC ATCTGGGCCG TCATCAAACC GGGTACATCC 
TTTGGAATCA GCGTGTTACG AGCTCTCAGG TTACTGCGTA TTTTCAAAGT CACAAAGTAC 
TGGGCATCTC TCAGAAACCT GGTTGTCTCC CTCCTCAACT CCATGAAATC CATCATAAGT 
CTGCTGTTCC TCCTCTTCCT CTTCATTGTC GTCTTTGCCC TCTTGGGGAT GCAGCTGTTT 
GGTGGCCAGT TTAATTTTGA CGAGGGGACT CCTCCCACCA ACTTCGACAC TTTTCCAGCA 
GCAATAATGA CTGTGTTTCA GATCCTGACT GGCGAGGATT GGAATGAGGT CATGTATGAT 
GGGATCAAGT CTCAGGGGGG CGTGCAGGGC GGCATGGTGT TCTCCATCTA CTTCATCGTC 
CTCACCCTCT TCGGGAACTA CACCCTGCTG AACGTGTTCT TGGCTATCGC GGTGGACAAC 
CTGGCCAACG CCCAGGAACT CACCAAGGAT GAACAAGAAG AGGAAGAGGC AGCCAATCAG 
AAACTGGCTC TACAGAAAGC CAAGGAGGTG GCAGAAGTGA GTCCCCTGTC TGCAGCCAAC 
ATGTCCATAG CTGTGAAGGA ACAGCAGAAG AACCAGAAGC CTGCCAAGTC GGTGTGGGAG 
CAGCGCACCA GCGAGATGCG CAAGCAGAAC CTGCTGGCTA GCCGCGAGGC GCTGTACGGG 
GACGCGGCTG AGCGCTGGCC CACCACTTAC GCGCGCCCGC TGCGGCCGGA CGTGAAGACG 
CACTTGGACC GGCCGCTCGT GGTGGACCCG CAGGAGAACC GTAACAACAA CACCAACAAG 
AGCCGTGCGC CAGAAGCGCT GCGCCAAACC GCGCGGCCCC GCGAGAGCGC GCGCGACCCC 
GACGCGCGGC GCGCCTGGCC CAGCAGCCCT GAGCGCGCCC CTGGACGAGA GGGCCCGTAT 
GGCCGCGAGA GCGAGCCGCA ACAGCGCGAG CACGCGCCAC CCCGCGAGCA CGTACCCTGG 
GACGCGGATC CTGAGCGCGC CAAGGCCGGG GACGCGCCCC GCCGCCACAC GCACCGGCCT 
GTGGCCGAGG GCGAGCCTCG TCGCCACCGC GCGCGCCGCC GGCCCGGGGA CGAACCGGAC 
GACAGACCGG AGCGCAGGCC GCGTCCCCGC GACGCCACTA GGCCGGCCCG CGCTGCAGAC 
GGCGAAGGCG ATGATGGGGA GCGCAAGCGG CGACACCGAC ACGGGCCGCC GGCCCACGAT 
GACAGGGAGC GCAGACACCG GCGCAGAAAA GAGAGCCAGG GCTCTGGGGT CCCCATGTCT 
GGTCCCAACC TGTCCACCAC CAGGCCAATC CAGCAGGATC TGGGCCGCCA GGACCTGCCA 
CTGGCTGAGG ACCTGGACAA CATGAAGAAC AACAAGTTGG CCACCGGGGA GCCTGCCAGT 
CCCCACGACA GCCTGGGCCA CAGTGGCCTT CCCCCTAGCC CTGCCAAGAT CGGGAACAGC 
ACCAACCCTG GTCCCGCCTT GGCCACCAAT CCCCAGAATG CTGCCAGCCG CAGGACGCCC 
AACAACCCGG GCAACCCGTC CAACCCCGGC CCCCCCAAGA CTCCCGAGAA CAGCCTTATC 
GTCACCAACC CCAGCAGCAC CCAGCCCAAC TCAGCAAAGA CTGCCAGGAA ACCCGAGCAC 
ATGGCGGTGG AGATCCCCCC GGCCTGCCCG CCCCTCAACC ACACTGTGGT CCAAGTAAAC 
AAAAACGCCA ACCCAGACCC ACTGCCAAAG AAAGAGGAAG AGAAGAAGGA GGAAGAGGAG 
GCAGACCCCG GGGAGGATGG CCCAAAGCCC ATGCCGCCCT ACAGCTCCAT GTTCATCCTC 
TCCACCACCA ACCCCCTTCG CCGGCTGTGC CATTACATCC TGAACCTGCG CTACTTCGAG 
ATGTGCATCC TCATGGTCAT TGCCATGAGT AGCATCGCGC TGGCCGCCGA GGACCCGGTG 
CAGCCCAACG CACCCCGCAA CAACGTGCTG CGATATTTTG ACTATGTTTT CACAGGAGTG 
TTTACCTTTG AGATGGTGAT CAAGATGATC GACCTGGGCC TCGTCCTGCA TCAGGGGGCC 
TATTTCCGTG ACCTGTGGAA CATTCTGGAC TTCATAGTGG TCAGTGGGGC CCTGGTGGCC 
TTTGCCTTCA CGAGCAGTAC ACGTGGCAAT AGCAAAGGAA AGGACATCAA CACCATCAAG 
TCCCTCCGAG TCCTCCGGGT GCTACGACCT CTAAAGACCA TCAAGCGGCT GCCTAAGTTG 
AAGGCCGTAT TTGACTGCGT GGTGAACTCG CTCAAGAACG TCTTCAACAT CCTCATTGTC 
TACATGCTCT TCATGTTCAT CTTCGCCGTG GTGGCCGTGC AGCTCTTCAA GGGCAAATTC 
TTCCACTGCA CGGACGAGTC CAAGGAGTTT GAGAGAGACT GTCGAGGCAA ATACCTCCTT 
TACGAGAAGA ACGAGGTAAA GGCGCGGGAC CGCGAGTGGA AGAAATACGA CTTCCACTAC 
GACAACGTGC TCTGGGCCCT GCTCACGCTC TTTACGGTGT CCACGGGAGA GGGCTGGCCA 
CAGGTCCTCA AGCACTCAGT GGATGCCACT TTTGAGAACC AGGGCCCCAG CCCCGGGTAC 
CGCATGGAAA TGTCCATCTT CTACGTGGTC TACTTTGTGG TGTTTCCCTT CTTCTTTGTC 
AATATCTTTG TGGCCTTGAT CATCATCACC TTCCAGGAGC AGGGAGACAA GATGATGGAA 
GAATACAGCC TAGAGAAAAA TGAGAGGGCC TGCATCGACT TTGCCATCAG TGCCAAGCCG 
CTGACCAGGC ACATGCCCCA GAACAAGCAG AGCTTCCAGT ATCGAATGTG GCAGTTCGTG 
GTGTCCCCAC CCTTTGAGTA CACCATCATG GCCATGATCG CTCTCAACAC CATCGTGCTA 
ATGATGAAGT TCTATGGAGC CTCTGTGGCC TATGAAAACG CCCTTCGAGT GTTCAACATT 
GTCTTCACCT CCCTCTTCTC TCTCGAATGT GTGCTCAAAG TCATGGCTTT TGGGATTCTG 
AATTATTTCC GCGATGCCTG GAACATCTTC GACTTTGTGA CTGTTCTGGG CAGCATCACA 
GACATCCTCG TCACCGAGTT TGGGAATCCG AATAACTTCA TCAACCTGAG CTTTCTCCGC 
CTCTTCCGTG CTGCCCGACT CATCAAACTC CTCCGCCAGG GTTACACCAT CCGCATTCTC 
CTCTGGACTT TCGTGCAGTC TTTCAAGGCC CTACCTTATG TCTGTCTGCT GATCGCCATG 
CTCTTCTTCA TCTATGCCAT CATCGGGATG CAGGTGATCC CGCACCCTTG GGGTACCGGG 
GCTGCGGGAG GGAGCTTGTG TCAAAGGGAG CCAAGGTGGC TGTGGCTGCT CCCATCGTCA 
CCCCCCCCCC CAACTCTCCA GGTGTTTGGC AACATCGGCA TTGATGGGGA AGATGAGGAC 
AGCGATGAGG ATGAGTTCCA AATCACGGAG CACAATAACT TCCGGACCTT CTTCCAAGCT 
CTCATGCTTC TCTTCCGGAG CGCCACAGGG GAAGCGTGGC ACAACATCAT GCTGTCCTGC 
CTCAGCGGGA AGCCATGCGA CAAGAACTCC GGGATCCAAA AACCAGAGTG TGGCAACGAG 
TTCGCCTATT TTTACTTTGT CTCGTTCATC TTCCTTTGCT CATTTCTGAT GCTGAATCTC 
TTTGTTGCTG TCATCATGGA CAACTTCGAG TACCTCACCC GAGATTCCTC CATCCTGGGC 
CCCCACCACC TGGATGAGTA CGTGCGTGTC TGGGCAGAGT ATGACCCTGC TGCCTGCGGC 
CGCATTCACT ATAAGGACAT GTACAGTTTA TTGCGAGTAA TATCGCCCCC TCTCGGCTTA 
GGCAAGAAAT GTCCTCATAG GGTTGCTTGC AAGAGGCTCT TGCGGATGGA CCTACCCGTA 
GCGGATGACA ACACCGTTCA CTTCAACTCC ACCTTGATGG CTCTGATCCG AACCGCCCTG 
GATATCAAAA TCGCAAAGGG TGGAGCTGAC AAGCAGCAAA TGGACGCAGA GCTCCGCAAG 
GAAATGATGG CCATTTGGCC CAACCTGTCT CAGAAGACCT TGGATCTGCT GGTCACACCT 
CACAAGTCCA CGGACCTGAC AGTGGGTAAG ATCTACGCAG CCATGATGAT CATGGAGTAC 
TACCGGCAGA GCAAGGCCAA GAAGCTGCAG GCCATGCGAG AGGAGCAGAA CCGGACACCA 
CTCATGTTCC AGCGCATGGA GCCTCCATCG CCAACACAGG AGGGAGGACC CAGCCAAAAC 
GCCCTTCCCT CCACTCAGCT GGACCCCGGA GGAGGCCTGA TGGCTCAAGA AAGCAGCATG 
AAGGAGAGCC CGTCCTGGGT GACCCAGCGG GCACAGGAGA TGTTCCAGAA GACTGGTACC 
TGGAGCCCAG AGCGAGGGCC ACCCATCGAC ATGCCTAACA GCCAGCCCAA CTCCCAGTCT 
GTGGAGATGA GAGAAATGGG AACTGATGGC TACTCAGACA GCGAACACTA CCTCCCCATG 
GAAGGACAGA CCAGGGCCGC CTCCATGCCC CGCCTCCCAG CAGAGAACCA GAGGAGAAGG 
GGCCGGCCAC GTGGAAATAA CCTCAGTACC ATCTCTGATA CCAGCCCCAT GAAGCGCTCA 
GCCTCCGTGC TGGGACCCAA AGCCCGGCGA CTGGATGACT ACTCACTAGA GCGGGTACCA 
CCTGAGGAGA ACCAAAGGTA CCACCAACGC CGCCGGGACC GTGGCCACCG CACCTCTGAG 
CGCTCTCTGG GCCGATACAC TGATGTGGAC ACAGGCCTGG GGACAGATCT GAGCATGACC 
ACCCAATCGG GTGACCTGCC CTCCAAAGAT CGGGACCAGG ACCGGGGCCG GCCCAAGGAC 
CGGAAGCATC GGCCACACCA CCACCACCAC CATCATCACC ATCATCCCCC GGCCCCGGAC 
CGGGAGCGCT ACGCACAGGA GCGGCCGGAC ACCGGCCGGG CGCGGGCCCG GGAGCAGCGC 
TGGTCCCGCT CGCCCAGCGA GGGTCGGGAG CACGCGACAC ACAGACAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Alcohol withdrawal upregulates mRNA encoding for Ca
Alcohol (Fayetteville, N.Y.)6621-26(2018 Feb)
Newton J,Suman S,Akinfiresoye LR,Datta K,Lovinger DM,N'Gouemo P

A CaV2.1 N-terminal fragment relieves the dominant-negative inhibition by an Episodic ataxia 2 mutant.
Neurobiology of disease93243-56(2016 Sep)
Dahimene S,Page KM,Nieto-Rostro M,Pratt WS,D'Arco M,Dolphin AC

A Single Amino Acid Deletion (?F1502) in the S6 Segment of CaV2.1 Domain III Associated with Congenital Ataxia Increases Channel Activity and Promotes Ca2+ Influx.
PloS one10(12)e0146035(2015)
Bahamonde MI,Serra SA,Drechsel O,Rahman R,Marc?-Grau A,Prieto M,Ossowski S,Macaya A,Fern?ndez-Fern?ndez JM

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