Prpf8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Gene Symbol	Prpf8
Entrez Gene ID	287530
Full Name	pre-mRNA processing factor 8
General protein information	Preferred Names pre-mRNA processing factor 8 Names pre-mRNA-processing-splicing factor 8 PRP8 pre-mRNA processing factor 8 homolog
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q24

mRNA and Protein(s)

mRNA Protein Name

XM_006246892.3 XP_006246954.1 pre-mRNA-processing-splicing factor 8 isoform X1

NM_001191590.1 NP_001178519.1 pre-mRNA-processing-splicing factor 8
Pathways from BioSystems

mRNA	Protein	Name
XM_006246892.3	XP_006246954.1	pre-mRNA-processing-splicing factor 8 isoform X1
NM_001191590.1	NP_001178519.1	pre-mRNA-processing-splicing factor 8

Homologies

Rattus norvegicus (Norway rat)	Prpf8	NP_001178519.1
Neurospora crassa	NCU07832	XP_962953.1
Arabidopsis thaliana (thale cress)	SUS2	NP_178124.2
	PRPF8	XP_001117328.1
Canis lupus familiaris (dog)	PRPF8	XP_854384.1
Drosophila melanogaster (fruit fly)	Prp8	NP_610735.1
Caenorhabditis elegans (roundworm)	prp-8	NP_498785.1
Schizosaccharomyces pombe (fission yeast)	spp42	NP_593861.1
Magnaporthe oryzae (rice blast fungus)	MGG_05244	XP_003712798.1
Oryza sativa (rice)	Os06g0167000	NP_001056917.1
Homo sapiens (human)	PRPF8	NP_006436.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006885	XP_308873.2
Kluyveromyces lactis	KLLA0A05280g	XP_451233.1
Eremothecium gossypii	AGOS_ABR207W	NP_983156.2
Bos taurus (cattle)	PRPF8	NP_001099108.1
Mus musculus (house mouse)	Prpf8	NP_619600.2
Arabidopsis thaliana (thale cress)	AT4G38780	NP_195589.2
Xenopus tropicalis (tropical clawed frog)	prpf8	XP_002936105.1
Gallus gallus (chicken)	PRPF8	XP_415805.2
Danio rerio (zebrafish)	prpf8	NP_957270.2
Saccharomyces cerevisiae (baker's yeast)	PRP8	NP_012035.1

Gene Ontology
Bibliography

Related articles in PubMed

Structure of a human catalytic step I spliceosome.
Zhan X, Yan C, Zhang X, Lei J, Shi Y
Science (New York, N.Y.)(2018 Jan)

Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for Activation.
Bertram K, Agafonov DE, Dybkov O, Haselbach D, Leelaram MN, Will CL, Urlaub H, Kastner B, L?hrmann R, Stark H
Cell170(4)701-713.e11(2017 Aug)

Cryo-EM structure of a human spliceosome activated for step 2 of splicing.
Bertram K, Agafonov DE, Liu WT, Dybkov O, Will CL, Hartmuth K, Urlaub H, Kastner B, Stark H, L?hrmann R
Nature542(7641)318-323(2017 02)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Prpf8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prpf8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa07077	NM_001191590.1 Latest version!	Rattus norvegicus pre-mRNA processing factor 8 (Prpf8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
ORa07077	XM_006246892.3 Latest version!	Rattus norvegicus pre-mRNA processing factor 8 (Prpf8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00

ORF Online Only Promotion

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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prpf8 ( NM_001191590.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001178519.1 Rattus norvegicus pre-mRNA processing factor 8 (Prpf8), mRNA.

Price & Availability↑

CloneID	ORa07077
Clone ID Related Accession (Same CDS sequence)	XM_006246892.3 , NM_001191590.1
Accession Version	NM_001191590.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Rattus norvegicus(Norway rat)
Product	pre-mRNA-processing-splicing factor 8
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AABR07030039.1. On Jul 17, 2010 this sequence version replaced XM_213385.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMEA2689603 [ECO:0000348] ##Evidence-Data-END##

ATGGCCGGAG TGTTTCCTTA CAGAGGGCCG GGTAACCCGG TGCCCGGCCC TCTAGCCCCG 
CTGCCGGATT ACATGTCAGA GGAGAAGCTG CAGGAGAAAG CTCGAAAATG GCAGCAGTTG 
CAGGCCAAGC GCTATGCAGA GAAGCGGAAA TTCGGGTTTG TGGATGCTCA GAAGGAAGAC 
ATGCCCCCAG AACATGTCAG GAAAATCATT CGAGACCATG GAGACATGAC CAACAGGAAG 
TTCCGTCATG ACAAAAGGGT CTATTTGGGA GCTCTCAAGT ACATGCCTCA CGCAGTTCTC 
AAGCTCCTAG AGAACATGCC CATGCCTTGG GAGCAGATCC GGGATGTGCC TGTGCTGTAC 
CACATCACTG GAGCCATTTC CTTCGTTAAC GAGATTCCCT GGGTTATTGA GCCAGTTTAC 
ATCTCCCAGT GGGGGTCAAT GTGGATCATG ATGCGTAGAG AAAAAAGAGA CAGGAGGCAT 
TTCAAGAGGA TGCGTTTTCC CCCTTTTGAT GATGAGGAGC CGCCCCTGGA TTATGCTGAC 
AACATCTTAG ATGTCGAGCC ACTGGAAGCC ATTCAGCTAG AGCTGGACCC TGAGGAGGAT 
GCCCCTGTGT TGGACTGGTT CTATGACCAC CAGCCATTGA GAGATAGTAG GAAGTATGTT 
AATGGTTCCA CCTACCAACG CTGGCAGTTC ACATTGCCTA TGATGTCCAC TCTCTACCGA 
CTGGCCAACC AGCTTCTGAC TGACTTGGTA GATGACAACT ACTTTTATCT CTTTGATCTA 
AAGGCCTTCT TTACCTCTAA GGCACTCAAC ATGGCCATTC CTGGGGGACC CAAATTTGAA 
CCTCTTGTTC GAGACATCAA CCTACAGGAT GAAGACTGGA ATGAATTCAA TGATATTAAC 
AAGATCATCA TCCGTCAGCC TATTCGTACT GAGTACAAGA TTGCTTTTCC GTACCTCTAT 
AACAACCTCC CACACCATGT CCACCTGACC TGGTACCACA CTCCTAACGT TGTGTTCATC 
AAGACTGAGG ATCCTGATCT GCCAGCTTTC TACTTTGATC CTCTGATTAA CCCAATCTCC 
CATAGACACT CAGTCAAGAG CCAAGAGCCA CTACCTGATG ATGATGAGGA GTTTGAGCTC 
CCAGAGTTTG TGGAGCCCTT CCTGAAGGAT ACACCTCTCT ATACAGACAA TACAGCCAAT 
GGCATCGCAC TGCTCTGGGC ACCACGGCCC TTCAACCTAC GCTCTGGTCG TACCCGCCGG 
GCCCTCGACA TTCCCCTTGT CAAGAACTGG TATCGGGAGC ACTGTCCTGC TGGGCAACCT 
GTGAAAGTTC GAGTCTCCTA TCAGAAGCTG CTTAAGTATT ATGTGCTGAA TGCCCTCAAA 
CATCGGCCTC CCAAAGCGCA GAAGAAGAGG TATCTGTTTC GCTCTTTCAA AGCTACCAAA 
TTCTTTCAGT CTACCAAGCT GGACTGGGTG GAGGTCGGGC TGCAGGTTTG CCGCCAGGGC 
TACAACATGC TTAACCTTCT CATTCACCGT AAGAACCTCA ACTATCTGCA TTTGGACTAT 
AACTTCAACC TGAAGCCTGT CAAGACGCTC ACCACCAAGG AAAGAAAAAA ATCTCGCTTT 
GGGAATGCTT TCCATCTGTG TCGGGAAGTA CTGCGTTTGA CTAAGCTGGT TGTGGATAGT 
CATGTGCAGT ATCGCTTGGG CAATGTAGAT GCCTTCCAGC TGGCAGATGG ATTGCAGTAT 
ATATTTGCCC ACGTGGGACA GTTGACAGGC ATGTATCGAT ACAAATATAA GCTGATGCGG 
CAGATCCGCA TGTGCAAGGA CTTGAAACAT CTCATCTATT ACCGCTTCAA CACGGGTCCT 
GTGGGGAAAG GCCCTGGCTG TGGTTTCTGG GCTGCTGGCT GGCGGGTCTG GCTATTTTTT 
ATGCGTGGAA TTACCCCTTT GCTAGAACGA TGGCTAGGCA ACCTTCTGGC CCGACAGTTT 
GAAGGCCGTC ACTCAAAGGG GGTGGCAAAG ACAGTAACAA AGCAACGAGT AGAGTCCCAT 
TTTGACCTTG AGCTTAGGGC AGCTGTGATG CATGATATTC TGGACATGAT GCCTGAAGGC 
ATCAAACAGA ACAAGGCCCG AACAATTCTG CAGCACCTCA GTGAGGCCTG GCGCTGCTGG 
AAGGCCAACA TTCCATGGAA GGTCCCAGGA CTGCCAACGC CTATAGAGAA TATGATTCTT 
CGGTACGTTA AGGCCAAGGC TGATTGGTGG ACCAATACTG CCCACTACAA CCGTGAACGG 
ATTCGCCGTG GGGCTACTGT AGACAAGACT GTTTGCAAAA AGAACCTGGG CCGCCTCACC 
CGCCTGTATC TGAAGGCAGA ACAGGAGCGG CAGCACAATT ACCTGAAGGA CGGGCCTTAC 
ATCACAGCAG AGGAAGCAGT GGCAGTGTAT ACTACCACTG TGCACTGGCT GGAAAGCCGT 
AGGTTCTCTC CTATCCCGTT CCCCCCACTC TCCTACAAGC ATGACACCAA GTTGCTTATC 
TTGGCTCTGG AGCGACTCAA GGAAGCTTAT AGTGTGAAGT CTAGGTTAAA CCAGTCTCAG 
AGGGAGGAGC TGGGTCTGAT TGAACAAGCC TATGACAACC CCCATGAGGC TTTGTCCCGC 
ATCAAGCGTC ACCTCCTCAC GCAGAGAGCC TTTAAGGAAG TGGGCATTGA GTTTATGGAT 
CTCTATAGCC ACCTTGTGCC AGTGTATGAT GTGGAACCAC TGGAGAAAAT AACTGATGCT 
TACCTGGACC AGTACCTGTG GTATGAAGCA GATAAACGCC GTCTTTTCCC GCCCTGGATC 
AAGCCTGCTG ACACAGAACC CCCTCCACTG CTTGTTTACA AGTGGTGCCA AGGCATCAAT 
AACCTGCAGG ATGTGTGGGA GACGAGTGAG GGTGAGTGCA ATGTCATGCT GGAGTCTCGG 
TTTGAGAAGA TGTATGAGAA GATTGACCTG ACCCTGCTCA ACAGGCTGCT GCGACTCATT 
GTGGACCACA ACATAGCTGA CTACATGACA GCCAAGAACA ATGTAGTCAT CAACTATAAG 
GACATGAACC ATACAAATTC ATATGGAATC ATCAGAGGCC TGCAGTTTGC CTCATTCATT 
GTACAGTACT ATGGTTTGGT GATGGATTTG CTTGTATTGG GATTGCACCG GGCCAGTGAG 
ATGGCTGGGC CTCCGCAGAT GCCAAATGAC TTTCTCAGTT TTCAGGACAT AGCCACCGAG 
GCTGCACACC CAATCCGACT CTTCTGTAGA TACATTGATC GCATCCATAT TTTCTTCAGG 
TTCACAGCAG ATGAGGCTCG GGATCTGATC CAGCGTTACC TGACAGAACA TCCAGACCCC 
AATAATGAAA ACATTGTTGG CTACAATAAT AAGAAATGCT GGCCCCGAGA TGCCCGTATG 
CGTCTCATGA AGCATGATGT CAACTTGGGT CGGGCAGTGT TCTGGGACAT CAAAAACCGG 
CTGCCACGAT CGGTGACTAC AGTACAGTGG GAGAATAGCT TTGTGTCTGT GTACAGTAAA 
GACAACCCCA ACCTGCTGTT CAACATGTGT GGCTTTGAGT GCCGCATCCT ACCTAAGTGC 
CGTACCAGCT ATGAAGAGTT CACCCACAAG GACGGGGTCT GGAATCTACA GAATGAGGTT 
ACTAAGGAGC GGACGGCTCA GTGTTTCCTG CGTGTGGACG ATGAGTCAAT GCAGCGCTTC 
CATAACCGCG TGCGGCAGAT TCTGATGGCC TCTGGCTCTA CCACTTTCAC CAAGATTGTG 
AATAAGTGGA ATACAGCTCT CATTGGCCTT ATGACATACT TTCGAGAGGC TGTGGTGAAT 
ACACAGGAAC TCCTAGACTT ACTGGTGAAG TGTGAAAACA AGATCCAGAC ACGTATCAAG 
ATTGGATTGA ACTCCAAGAT GCCAAGTCGG TTCCCCCCTG TTGTATTCTA CACCCCTAAG 
GAGCTGGGTG GGCTTGGCAT GCTCTCGATG GGCCATGTCC TTATCCCCCA GTCTGACCTC 
AGGTGGTCCA AGCAAACAGA TGTGGGCATC ACACACTTTC GTTCAGGAAT GAGTCATGAA 
GAAGACCAAC TGATTCCCAA CTTGTACCGC TACATACAGC CATGGGAGAG TGAATTCATT 
GACTCCCAGC GGGTCTGGGC TGAGTATGCA CTCAAGCGAC AGGAGGCTAT TGCTCAGAAC 
AGACGCTTGA CTTTAGAAGA TTTAGAAGAC TCGTGGGATC GGGGCATTCC CCGGATCAAT 
ACCCTCTTCC AGAAAGATCG GCACACATTG GCTTATGATA AAGGCTGGCG TGTCCGAACT 
GACTTTAAGC AGTACCAGGT TTTGAAGCAG AACCCCTTTT GGTGGACACA TCAACGGCAT 
GATGGCAAGC TTTGGAACCT CAACAACTAC CGCACAGACA TGATCCAGGC ACTGGGCGGT 
GTGGAGGGCA TTCTGGAACA CACTCTCTTC AAGGGCACTT ACTTCCCAAC CTGGGAAGGT 
CTGTTCTGGG AGAAAGCCAG CGGGTTTGAG GAATCCATGA AGTGGAAGAA GCTGACCAAT 
GCTCAGCGAT CAGGATTAAA CCAGATTCCT AATCGTAGAT TTACTCTCTG GTGGTCCCCA 
ACAATCAATC GAGCCAATGT ATACGTAGGC TTCCAGGTGC AGCTGGACCT GACAGGAATC 
TTTATGCATG GCAAGATCCC CACGCTGAAG ATCTCTCTCA TCCAGATCTT CCGAGCTCAC 
TTGTGGCAAA AGATTCATGA GAGCATCGTG ATGGACTTGT GTCAGGTCTT CGACCAGGAA 
TTAGATGCCC TGGAAATTGA GACAGTACAA AAGGAGACAA TCCATCCCCG AAAATCCTAC 
AAGATGAACT CTTCCTGTGC AGATATACTG CTCTTTGCTT CCTATAAGTG GAATGTCTCC 
CGGCCCTCGC TGCTGGCAGA CTCTAAGGAT GTGATGGACA GCACCACCAC ACAGAAGTAT 
TGGATTGACA TCCAGTTACG CTGGGGGGAC TATGATTCCC ATGATATTGA GCGCTATGCC 
CGGGCCAAGT TCCTGGATTA CACCACAGAC AACATGAGTA TCTACCCTTC ACCCACAGGT 
GTGCTCATTG CCATTGATCT GGCCTATAAT CTACACAGTG CCTATGGAAA CTGGTTCCCG 
GGGAGCAAGC CTCTCATACA GCAAGCCATG GCCAAGATCA TGAAGGCAAA CCCTGCCTTG 
TATGTGTTAC GTGAACGGAT ACGCAAAGGA TTGCAGCTGT ATTCATCTGA GCCCACTGAG 
CCTTACCTGT CCTCTCAGAA CTATGGTGAA CTCTTCTCGA ACCAGATTAT CTGGTTTGTG 
GATGACACCA ATGTCTATAG AGTAACTATC CACAAGACCT TTGAGGGGAA CTTGACAACC 
AAACCCATCA ATGGGGCCAT CTTCATCTTC AATCCACGCA CAGGACAGCT GTTCCTCAAG 
ATAATCCACA CATCCGTATG GGCTGGACAG AAGCGTTTGG GTCAGTTGGC CAAGTGGAAG 
ACTGCTGAAG AAGTGGCTGC CCTGATCCGA TCTCTGCCTG TGGAGGAACA GCCTAAACAG 
ATTATTGTCA CTCGGAAAGG GATGCTGGAT CCCCTGGAGG TACACTTACT GGACTTCCCC 
AATATTGTGA TCAAAGGATC AGAGCTCCAG CTGCCCTTCC AGGCTTGTCT CAAGGTGGAG 
AAGTTTGGGG ATCTCATCCT TAAAGCCACA GAACCACAGA TGGTTCTCTT CAACCTCTAT 
GATGACTGGC TCAAGACTAT CTCATCCTAC ACGGCTTTCT CCCGTCTCAT CCTGATCCTG 
CGTGCTTTGC ATGTGAACAA CGATAGGGCA AAAGTGATCC TGAAGCCAGA CAAGACAACC 
GTTACGGAAC CCCACCACAT CTGGCCCACG CTCACTGACG AGGAGTGGAT CAAGGTGGAG 
GTGCAGCTCA AGGATCTGAT CTTGGCTGAC TATGGCAAGA AAAACAATGT GAATGTGGCA 
TCGCTGACAC AATCAGAAAT TCGAGACATC ATCCTGGGAA TGGAGATCTC TGCACCATCG 
CAGCAGCGGC AGCAGATAGC TGAGATTGAG AAGCAGACCA AGGAACAGTC CCAGCTCACT 
GCTACACAGA CTCGAACTGT CAACAAGCAT GGCGATGAGA TCATCACCTC CACCACTAGC 
AACTATGAGA CCCAGACCTT CTCGTCGAAG ACCGAATGGA GAGTCAGGGC CATCTCTGCT 
GCCAACCTGC ATCTACGGAC CAATCACATT TATGTTTCAT CTGATGACAT CAAGGAGACT 
GGTTATACGT ACATTCTTCC CAAAAATGTG CTGAAGAAGT TCATCTGCAT ATCTGATCTG 
CGCGCCCAAA TTGCAGGATA CCTATATGGG GTGAGCCCTC CAGATAACCC TCAGGTGAAG 
GAGATCCGCT GTATTGTGAT GGTGCCACAG TGGGGCACTC ACCAGACTGT GCACCTGCCT 
AGTCAGCTGC CTCAACATGA GTACCTCAAG GAGATGGAAC CCTTAGGTTG GATCCACACT 
CAACCCAATG AATCCCCCCA GCTATCGCCC CAAGATGTAA CTACTCATGC CAAAATCATG 
GCTGACAACC CATCTTGGGA TGGGGAGAAG ACTATTATCA TCACCTGCAG CTTCACACCA 
GGTTCCTGCA CACTGACAGC CTACAAGCTG ACCCCTAGCG GGTATGAGTG GGGTCGGCAG 
AACACAGACA AAGGCAACAA CCCCAAAGGC TACCTACCTT CGCACTATGA GAGGGTTCAG 
ATGCTCCTGT CTGACCGATT CCTCGGCTTC TTTATGGTCC CTGCCCAGTC CTCCTGGAAC 
TACAACTTTA TGGGTGTTCG ACATGACCCC AACATGAAAT ACGAGCTGCA GCTAGCAAAC 
CCCAAGGAGT TCTACCATGA GGTCCACAGG CCCTCCCACT TCCTCAACTT TGCCCTCCTG 
CAGGAGGGTG AGGTCTATTC CGCAGACAGA GAGGACCTCT ATGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178519.1
CDS	82..7089
Translation	MAGVFPYRGPGNPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKEDMPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRKYVNGSTYQRWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPISHRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRRALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCWKANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLTRLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLILALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWETSEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATEAAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKCRTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTVTEPHHIWPTLTDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPSQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWNYNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA

Target ORF information:

RefSeq Version	NM_001191590.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus pre-mRNA processing factor 8 (Prpf8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191590.1

ORF Insert Sequence:

ATGGCCGGAG TGTTTCCTTA CAGAGGGCCG GGTAACCCGG TGCCCGGCCC TCTAGCCCCG 
CTGCCGGATT ACATGTCAGA GGAGAAGCTG CAGGAGAAAG CTCGAAAATG GCAGCAGTTG 
CAGGCCAAGC GCTATGCAGA GAAGCGGAAA TTCGGGTTTG TGGATGCTCA GAAGGAAGAC 
ATGCCCCCAG AACATGTCAG GAAAATCATT CGAGACCATG GAGACATGAC CAACAGGAAG 
TTCCGTCATG ACAAAAGGGT CTATTTGGGA GCTCTCAAGT ACATGCCTCA CGCAGTTCTC 
AAGCTCCTAG AGAACATGCC CATGCCTTGG GAGCAGATCC GGGATGTGCC TGTGCTGTAC 
CACATCACTG GAGCCATTTC CTTCGTTAAC GAGATTCCCT GGGTTATTGA GCCAGTTTAC 
ATCTCCCAGT GGGGGTCAAT GTGGATCATG ATGCGTAGAG AAAAAAGAGA CAGGAGGCAT 
TTCAAGAGGA TGCGTTTTCC CCCTTTTGAT GATGAGGAGC CGCCCCTGGA TTATGCTGAC 
AACATCTTAG ATGTCGAGCC ACTGGAAGCC ATTCAGCTAG AGCTGGACCC TGAGGAGGAT 
GCCCCTGTGT TGGACTGGTT CTATGACCAC CAGCCATTGA GAGATAGTAG GAAGTATGTT 
AATGGTTCCA CCTACCAACG CTGGCAGTTC ACATTGCCTA TGATGTCCAC TCTCTACCGA 
CTGGCCAACC AGCTTCTGAC TGACTTGGTA GATGACAACT ACTTTTATCT CTTTGATCTA 
AAGGCCTTCT TTACCTCTAA GGCACTCAAC ATGGCCATTC CTGGGGGACC CAAATTTGAA 
CCTCTTGTTC GAGACATCAA CCTACAGGAT GAAGACTGGA ATGAATTCAA TGATATTAAC 
AAGATCATCA TCCGTCAGCC TATTCGTACT GAGTACAAGA TTGCTTTTCC GTACCTCTAT 
AACAACCTCC CACACCATGT CCACCTGACC TGGTACCACA CTCCTAACGT TGTGTTCATC 
AAGACTGAGG ATCCTGATCT GCCAGCTTTC TACTTTGATC CTCTGATTAA CCCAATCTCC 
CATAGACACT CAGTCAAGAG CCAAGAGCCA CTACCTGATG ATGATGAGGA GTTTGAGCTC 
CCAGAGTTTG TGGAGCCCTT CCTGAAGGAT ACACCTCTCT ATACAGACAA TACAGCCAAT 
GGCATCGCAC TGCTCTGGGC ACCACGGCCC TTCAACCTAC GCTCTGGTCG TACCCGCCGG 
GCCCTCGACA TTCCCCTTGT CAAGAACTGG TATCGGGAGC ACTGTCCTGC TGGGCAACCT 
GTGAAAGTTC GAGTCTCCTA TCAGAAGCTG CTTAAGTATT ATGTGCTGAA TGCCCTCAAA 
CATCGGCCTC CCAAAGCGCA GAAGAAGAGG TATCTGTTTC GCTCTTTCAA AGCTACCAAA 
TTCTTTCAGT CTACCAAGCT GGACTGGGTG GAGGTCGGGC TGCAGGTTTG CCGCCAGGGC 
TACAACATGC TTAACCTTCT CATTCACCGT AAGAACCTCA ACTATCTGCA TTTGGACTAT 
AACTTCAACC TGAAGCCTGT CAAGACGCTC ACCACCAAGG AAAGAAAAAA ATCTCGCTTT 
GGGAATGCTT TCCATCTGTG TCGGGAAGTA CTGCGTTTGA CTAAGCTGGT TGTGGATAGT 
CATGTGCAGT ATCGCTTGGG CAATGTAGAT GCCTTCCAGC TGGCAGATGG ATTGCAGTAT 
ATATTTGCCC ACGTGGGACA GTTGACAGGC ATGTATCGAT ACAAATATAA GCTGATGCGG 
CAGATCCGCA TGTGCAAGGA CTTGAAACAT CTCATCTATT ACCGCTTCAA CACGGGTCCT 
GTGGGGAAAG GCCCTGGCTG TGGTTTCTGG GCTGCTGGCT GGCGGGTCTG GCTATTTTTT 
ATGCGTGGAA TTACCCCTTT GCTAGAACGA TGGCTAGGCA ACCTTCTGGC CCGACAGTTT 
GAAGGCCGTC ACTCAAAGGG GGTGGCAAAG ACAGTAACAA AGCAACGAGT AGAGTCCCAT 
TTTGACCTTG AGCTTAGGGC AGCTGTGATG CATGATATTC TGGACATGAT GCCTGAAGGC 
ATCAAACAGA ACAAGGCCCG AACAATTCTG CAGCACCTCA GTGAGGCCTG GCGCTGCTGG 
AAGGCCAACA TTCCATGGAA GGTCCCAGGA CTGCCAACGC CTATAGAGAA TATGATTCTT 
CGGTACGTTA AGGCCAAGGC TGATTGGTGG ACCAATACTG CCCACTACAA CCGTGAACGG 
ATTCGCCGTG GGGCTACTGT AGACAAGACT GTTTGCAAAA AGAACCTGGG CCGCCTCACC 
CGCCTGTATC TGAAGGCAGA ACAGGAGCGG CAGCACAATT ACCTGAAGGA CGGGCCTTAC 
ATCACAGCAG AGGAAGCAGT GGCAGTGTAT ACTACCACTG TGCACTGGCT GGAAAGCCGT 
AGGTTCTCTC CTATCCCGTT CCCCCCACTC TCCTACAAGC ATGACACCAA GTTGCTTATC 
TTGGCTCTGG AGCGACTCAA GGAAGCTTAT AGTGTGAAGT CTAGGTTAAA CCAGTCTCAG 
AGGGAGGAGC TGGGTCTGAT TGAACAAGCC TATGACAACC CCCATGAGGC TTTGTCCCGC 
ATCAAGCGTC ACCTCCTCAC GCAGAGAGCC TTTAAGGAAG TGGGCATTGA GTTTATGGAT 
CTCTATAGCC ACCTTGTGCC AGTGTATGAT GTGGAACCAC TGGAGAAAAT AACTGATGCT 
TACCTGGACC AGTACCTGTG GTATGAAGCA GATAAACGCC GTCTTTTCCC GCCCTGGATC 
AAGCCTGCTG ACACAGAACC CCCTCCACTG CTTGTTTACA AGTGGTGCCA AGGCATCAAT 
AACCTGCAGG ATGTGTGGGA GACGAGTGAG GGTGAGTGCA ATGTCATGCT GGAGTCTCGG 
TTTGAGAAGA TGTATGAGAA GATTGACCTG ACCCTGCTCA ACAGGCTGCT GCGACTCATT 
GTGGACCACA ACATAGCTGA CTACATGACA GCCAAGAACA ATGTAGTCAT CAACTATAAG 
GACATGAACC ATACAAATTC ATATGGAATC ATCAGAGGCC TGCAGTTTGC CTCATTCATT 
GTACAGTACT ATGGTTTGGT GATGGATTTG CTTGTATTGG GATTGCACCG GGCCAGTGAG 
ATGGCTGGGC CTCCGCAGAT GCCAAATGAC TTTCTCAGTT TTCAGGACAT AGCCACCGAG 
GCTGCACACC CAATCCGACT CTTCTGTAGA TACATTGATC GCATCCATAT TTTCTTCAGG 
TTCACAGCAG ATGAGGCTCG GGATCTGATC CAGCGTTACC TGACAGAACA TCCAGACCCC 
AATAATGAAA ACATTGTTGG CTACAATAAT AAGAAATGCT GGCCCCGAGA TGCCCGTATG 
CGTCTCATGA AGCATGATGT CAACTTGGGT CGGGCAGTGT TCTGGGACAT CAAAAACCGG 
CTGCCACGAT CGGTGACTAC AGTACAGTGG GAGAATAGCT TTGTGTCTGT GTACAGTAAA 
GACAACCCCA ACCTGCTGTT CAACATGTGT GGCTTTGAGT GCCGCATCCT ACCTAAGTGC 
CGTACCAGCT ATGAAGAGTT CACCCACAAG GACGGGGTCT GGAATCTACA GAATGAGGTT 
ACTAAGGAGC GGACGGCTCA GTGTTTCCTG CGTGTGGACG ATGAGTCAAT GCAGCGCTTC 
CATAACCGCG TGCGGCAGAT TCTGATGGCC TCTGGCTCTA CCACTTTCAC CAAGATTGTG 
AATAAGTGGA ATACAGCTCT CATTGGCCTT ATGACATACT TTCGAGAGGC TGTGGTGAAT 
ACACAGGAAC TCCTAGACTT ACTGGTGAAG TGTGAAAACA AGATCCAGAC ACGTATCAAG 
ATTGGATTGA ACTCCAAGAT GCCAAGTCGG TTCCCCCCTG TTGTATTCTA CACCCCTAAG 
GAGCTGGGTG GGCTTGGCAT GCTCTCGATG GGCCATGTCC TTATCCCCCA GTCTGACCTC 
AGGTGGTCCA AGCAAACAGA TGTGGGCATC ACACACTTTC GTTCAGGAAT GAGTCATGAA 
GAAGACCAAC TGATTCCCAA CTTGTACCGC TACATACAGC CATGGGAGAG TGAATTCATT 
GACTCCCAGC GGGTCTGGGC TGAGTATGCA CTCAAGCGAC AGGAGGCTAT TGCTCAGAAC 
AGACGCTTGA CTTTAGAAGA TTTAGAAGAC TCGTGGGATC GGGGCATTCC CCGGATCAAT 
ACCCTCTTCC AGAAAGATCG GCACACATTG GCTTATGATA AAGGCTGGCG TGTCCGAACT 
GACTTTAAGC AGTACCAGGT TTTGAAGCAG AACCCCTTTT GGTGGACACA TCAACGGCAT 
GATGGCAAGC TTTGGAACCT CAACAACTAC CGCACAGACA TGATCCAGGC ACTGGGCGGT 
GTGGAGGGCA TTCTGGAACA CACTCTCTTC AAGGGCACTT ACTTCCCAAC CTGGGAAGGT 
CTGTTCTGGG AGAAAGCCAG CGGGTTTGAG GAATCCATGA AGTGGAAGAA GCTGACCAAT 
GCTCAGCGAT CAGGATTAAA CCAGATTCCT AATCGTAGAT TTACTCTCTG GTGGTCCCCA 
ACAATCAATC GAGCCAATGT ATACGTAGGC TTCCAGGTGC AGCTGGACCT GACAGGAATC 
TTTATGCATG GCAAGATCCC CACGCTGAAG ATCTCTCTCA TCCAGATCTT CCGAGCTCAC 
TTGTGGCAAA AGATTCATGA GAGCATCGTG ATGGACTTGT GTCAGGTCTT CGACCAGGAA 
TTAGATGCCC TGGAAATTGA GACAGTACAA AAGGAGACAA TCCATCCCCG AAAATCCTAC 
AAGATGAACT CTTCCTGTGC AGATATACTG CTCTTTGCTT CCTATAAGTG GAATGTCTCC 
CGGCCCTCGC TGCTGGCAGA CTCTAAGGAT GTGATGGACA GCACCACCAC ACAGAAGTAT 
TGGATTGACA TCCAGTTACG CTGGGGGGAC TATGATTCCC ATGATATTGA GCGCTATGCC 
CGGGCCAAGT TCCTGGATTA CACCACAGAC AACATGAGTA TCTACCCTTC ACCCACAGGT 
GTGCTCATTG CCATTGATCT GGCCTATAAT CTACACAGTG CCTATGGAAA CTGGTTCCCG 
GGGAGCAAGC CTCTCATACA GCAAGCCATG GCCAAGATCA TGAAGGCAAA CCCTGCCTTG 
TATGTGTTAC GTGAACGGAT ACGCAAAGGA TTGCAGCTGT ATTCATCTGA GCCCACTGAG 
CCTTACCTGT CCTCTCAGAA CTATGGTGAA CTCTTCTCGA ACCAGATTAT CTGGTTTGTG 
GATGACACCA ATGTCTATAG AGTAACTATC CACAAGACCT TTGAGGGGAA CTTGACAACC 
AAACCCATCA ATGGGGCCAT CTTCATCTTC AATCCACGCA CAGGACAGCT GTTCCTCAAG 
ATAATCCACA CATCCGTATG GGCTGGACAG AAGCGTTTGG GTCAGTTGGC CAAGTGGAAG 
ACTGCTGAAG AAGTGGCTGC CCTGATCCGA TCTCTGCCTG TGGAGGAACA GCCTAAACAG 
ATTATTGTCA CTCGGAAAGG GATGCTGGAT CCCCTGGAGG TACACTTACT GGACTTCCCC 
AATATTGTGA TCAAAGGATC AGAGCTCCAG CTGCCCTTCC AGGCTTGTCT CAAGGTGGAG 
AAGTTTGGGG ATCTCATCCT TAAAGCCACA GAACCACAGA TGGTTCTCTT CAACCTCTAT 
GATGACTGGC TCAAGACTAT CTCATCCTAC ACGGCTTTCT CCCGTCTCAT CCTGATCCTG 
CGTGCTTTGC ATGTGAACAA CGATAGGGCA AAAGTGATCC TGAAGCCAGA CAAGACAACC 
GTTACGGAAC CCCACCACAT CTGGCCCACG CTCACTGACG AGGAGTGGAT CAAGGTGGAG 
GTGCAGCTCA AGGATCTGAT CTTGGCTGAC TATGGCAAGA AAAACAATGT GAATGTGGCA 
TCGCTGACAC AATCAGAAAT TCGAGACATC ATCCTGGGAA TGGAGATCTC TGCACCATCG 
CAGCAGCGGC AGCAGATAGC TGAGATTGAG AAGCAGACCA AGGAACAGTC CCAGCTCACT 
GCTACACAGA CTCGAACTGT CAACAAGCAT GGCGATGAGA TCATCACCTC CACCACTAGC 
AACTATGAGA CCCAGACCTT CTCGTCGAAG ACCGAATGGA GAGTCAGGGC CATCTCTGCT 
GCCAACCTGC ATCTACGGAC CAATCACATT TATGTTTCAT CTGATGACAT CAAGGAGACT 
GGTTATACGT ACATTCTTCC CAAAAATGTG CTGAAGAAGT TCATCTGCAT ATCTGATCTG 
CGCGCCCAAA TTGCAGGATA CCTATATGGG GTGAGCCCTC CAGATAACCC TCAGGTGAAG 
GAGATCCGCT GTATTGTGAT GGTGCCACAG TGGGGCACTC ACCAGACTGT GCACCTGCCT 
AGTCAGCTGC CTCAACATGA GTACCTCAAG GAGATGGAAC CCTTAGGTTG GATCCACACT 
CAACCCAATG AATCCCCCCA GCTATCGCCC CAAGATGTAA CTACTCATGC CAAAATCATG 
GCTGACAACC CATCTTGGGA TGGGGAGAAG ACTATTATCA TCACCTGCAG CTTCACACCA 
GGTTCCTGCA CACTGACAGC CTACAAGCTG ACCCCTAGCG GGTATGAGTG GGGTCGGCAG 
AACACAGACA AAGGCAACAA CCCCAAAGGC TACCTACCTT CGCACTATGA GAGGGTTCAG 
ATGCTCCTGT CTGACCGATT CCTCGGCTTC TTTATGGTCC CTGCCCAGTC CTCCTGGAAC 
TACAACTTTA TGGGTGTTCG ACATGACCCC AACATGAAAT ACGAGCTGCA GCTAGCAAAC 
CCCAAGGAGT TCTACCATGA GGTCCACAGG CCCTCCCACT TCCTCAACTT TGCCCTCCTG 
CAGGAGGGTG AGGTCTATTC CGCAGACAGA GAGGACCTCT ATGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prpf8 ( XM_006246892.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006246954.1 Rattus norvegicus pre-mRNA processing factor 8 (Prpf8), transcript variant X1, mRNA.

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CloneID	ORa07077
Clone ID Related Accession (Same CDS sequence)	XM_006246892.3 , NM_001191590.1
Accession Version	XM_006246892.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	pre-mRNA-processing-splicing factor 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006246892.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGAG TGTTTCCTTA CAGAGGGCCG GGTAACCCGG TGCCCGGCCC TCTAGCCCCG 
CTGCCGGATT ACATGTCAGA GGAGAAGCTG CAGGAGAAAG CTCGAAAATG GCAGCAGTTG 
CAGGCCAAGC GCTATGCAGA GAAGCGGAAA TTCGGGTTTG TGGATGCTCA GAAGGAAGAC 
ATGCCCCCAG AACATGTCAG GAAAATCATT CGAGACCATG GAGACATGAC CAACAGGAAG 
TTCCGTCATG ACAAAAGGGT CTATTTGGGA GCTCTCAAGT ACATGCCTCA CGCAGTTCTC 
AAGCTCCTAG AGAACATGCC CATGCCTTGG GAGCAGATCC GGGATGTGCC TGTGCTGTAC 
CACATCACTG GAGCCATTTC CTTCGTTAAC GAGATTCCCT GGGTTATTGA GCCAGTTTAC 
ATCTCCCAGT GGGGGTCAAT GTGGATCATG ATGCGTAGAG AAAAAAGAGA CAGGAGGCAT 
TTCAAGAGGA TGCGTTTTCC CCCTTTTGAT GATGAGGAGC CGCCCCTGGA TTATGCTGAC 
AACATCTTAG ATGTCGAGCC ACTGGAAGCC ATTCAGCTAG AGCTGGACCC TGAGGAGGAT 
GCCCCTGTGT TGGACTGGTT CTATGACCAC CAGCCATTGA GAGATAGTAG GAAGTATGTT 
AATGGTTCCA CCTACCAACG CTGGCAGTTC ACATTGCCTA TGATGTCCAC TCTCTACCGA 
CTGGCCAACC AGCTTCTGAC TGACTTGGTA GATGACAACT ACTTTTATCT CTTTGATCTA 
AAGGCCTTCT TTACCTCTAA GGCACTCAAC ATGGCCATTC CTGGGGGACC CAAATTTGAA 
CCTCTTGTTC GAGACATCAA CCTACAGGAT GAAGACTGGA ATGAATTCAA TGATATTAAC 
AAGATCATCA TCCGTCAGCC TATTCGTACT GAGTACAAGA TTGCTTTTCC GTACCTCTAT 
AACAACCTCC CACACCATGT CCACCTGACC TGGTACCACA CTCCTAACGT TGTGTTCATC 
AAGACTGAGG ATCCTGATCT GCCAGCTTTC TACTTTGATC CTCTGATTAA CCCAATCTCC 
CATAGACACT CAGTCAAGAG CCAAGAGCCA CTACCTGATG ATGATGAGGA GTTTGAGCTC 
CCAGAGTTTG TGGAGCCCTT CCTGAAGGAT ACACCTCTCT ATACAGACAA TACAGCCAAT 
GGCATCGCAC TGCTCTGGGC ACCACGGCCC TTCAACCTAC GCTCTGGTCG TACCCGCCGG 
GCCCTCGACA TTCCCCTTGT CAAGAACTGG TATCGGGAGC ACTGTCCTGC TGGGCAACCT 
GTGAAAGTTC GAGTCTCCTA TCAGAAGCTG CTTAAGTATT ATGTGCTGAA TGCCCTCAAA 
CATCGGCCTC CCAAAGCGCA GAAGAAGAGG TATCTGTTTC GCTCTTTCAA AGCTACCAAA 
TTCTTTCAGT CTACCAAGCT GGACTGGGTG GAGGTCGGGC TGCAGGTTTG CCGCCAGGGC 
TACAACATGC TTAACCTTCT CATTCACCGT AAGAACCTCA ACTATCTGCA TTTGGACTAT 
AACTTCAACC TGAAGCCTGT CAAGACGCTC ACCACCAAGG AAAGAAAAAA ATCTCGCTTT 
GGGAATGCTT TCCATCTGTG TCGGGAAGTA CTGCGTTTGA CTAAGCTGGT TGTGGATAGT 
CATGTGCAGT ATCGCTTGGG CAATGTAGAT GCCTTCCAGC TGGCAGATGG ATTGCAGTAT 
ATATTTGCCC ACGTGGGACA GTTGACAGGC ATGTATCGAT ACAAATATAA GCTGATGCGG 
CAGATCCGCA TGTGCAAGGA CTTGAAACAT CTCATCTATT ACCGCTTCAA CACGGGTCCT 
GTGGGGAAAG GCCCTGGCTG TGGTTTCTGG GCTGCTGGCT GGCGGGTCTG GCTATTTTTT 
ATGCGTGGAA TTACCCCTTT GCTAGAACGA TGGCTAGGCA ACCTTCTGGC CCGACAGTTT 
GAAGGCCGTC ACTCAAAGGG GGTGGCAAAG ACAGTAACAA AGCAACGAGT AGAGTCCCAT 
TTTGACCTTG AGCTTAGGGC AGCTGTGATG CATGATATTC TGGACATGAT GCCTGAAGGC 
ATCAAACAGA ACAAGGCCCG AACAATTCTG CAGCACCTCA GTGAGGCCTG GCGCTGCTGG 
AAGGCCAACA TTCCATGGAA GGTCCCAGGA CTGCCAACGC CTATAGAGAA TATGATTCTT 
CGGTACGTTA AGGCCAAGGC TGATTGGTGG ACCAATACTG CCCACTACAA CCGTGAACGG 
ATTCGCCGTG GGGCTACTGT AGACAAGACT GTTTGCAAAA AGAACCTGGG CCGCCTCACC 
CGCCTGTATC TGAAGGCAGA ACAGGAGCGG CAGCACAATT ACCTGAAGGA CGGGCCTTAC 
ATCACAGCAG AGGAAGCAGT GGCAGTGTAT ACTACCACTG TGCACTGGCT GGAAAGCCGT 
AGGTTCTCTC CTATCCCGTT CCCCCCACTC TCCTACAAGC ATGACACCAA GTTGCTTATC 
TTGGCTCTGG AGCGACTCAA GGAAGCTTAT AGTGTGAAGT CTAGGTTAAA CCAGTCTCAG 
AGGGAGGAGC TGGGTCTGAT TGAACAAGCC TATGACAACC CCCATGAGGC TTTGTCCCGC 
ATCAAGCGTC ACCTCCTCAC GCAGAGAGCC TTTAAGGAAG TGGGCATTGA GTTTATGGAT 
CTCTATAGCC ACCTTGTGCC AGTGTATGAT GTGGAACCAC TGGAGAAAAT AACTGATGCT 
TACCTGGACC AGTACCTGTG GTATGAAGCA GATAAACGCC GTCTTTTCCC GCCCTGGATC 
AAGCCTGCTG ACACAGAACC CCCTCCACTG CTTGTTTACA AGTGGTGCCA AGGCATCAAT 
AACCTGCAGG ATGTGTGGGA GACGAGTGAG GGTGAGTGCA ATGTCATGCT GGAGTCTCGG 
TTTGAGAAGA TGTATGAGAA GATTGACCTG ACCCTGCTCA ACAGGCTGCT GCGACTCATT 
GTGGACCACA ACATAGCTGA CTACATGACA GCCAAGAACA ATGTAGTCAT CAACTATAAG 
GACATGAACC ATACAAATTC ATATGGAATC ATCAGAGGCC TGCAGTTTGC CTCATTCATT 
GTACAGTACT ATGGTTTGGT GATGGATTTG CTTGTATTGG GATTGCACCG GGCCAGTGAG 
ATGGCTGGGC CTCCGCAGAT GCCAAATGAC TTTCTCAGTT TTCAGGACAT AGCCACCGAG 
GCTGCACACC CAATCCGACT CTTCTGTAGA TACATTGATC GCATCCATAT TTTCTTCAGG 
TTCACAGCAG ATGAGGCTCG GGATCTGATC CAGCGTTACC TGACAGAACA TCCAGACCCC 
AATAATGAAA ACATTGTTGG CTACAATAAT AAGAAATGCT GGCCCCGAGA TGCCCGTATG 
CGTCTCATGA AGCATGATGT CAACTTGGGT CGGGCAGTGT TCTGGGACAT CAAAAACCGG 
CTGCCACGAT CGGTGACTAC AGTACAGTGG GAGAATAGCT TTGTGTCTGT GTACAGTAAA 
GACAACCCCA ACCTGCTGTT CAACATGTGT GGCTTTGAGT GCCGCATCCT ACCTAAGTGC 
CGTACCAGCT ATGAAGAGTT CACCCACAAG GACGGGGTCT GGAATCTACA GAATGAGGTT 
ACTAAGGAGC GGACGGCTCA GTGTTTCCTG CGTGTGGACG ATGAGTCAAT GCAGCGCTTC 
CATAACCGCG TGCGGCAGAT TCTGATGGCC TCTGGCTCTA CCACTTTCAC CAAGATTGTG 
AATAAGTGGA ATACAGCTCT CATTGGCCTT ATGACATACT TTCGAGAGGC TGTGGTGAAT 
ACACAGGAAC TCCTAGACTT ACTGGTGAAG TGTGAAAACA AGATCCAGAC ACGTATCAAG 
ATTGGATTGA ACTCCAAGAT GCCAAGTCGG TTCCCCCCTG TTGTATTCTA CACCCCTAAG 
GAGCTGGGTG GGCTTGGCAT GCTCTCGATG GGCCATGTCC TTATCCCCCA GTCTGACCTC 
AGGTGGTCCA AGCAAACAGA TGTGGGCATC ACACACTTTC GTTCAGGAAT GAGTCATGAA 
GAAGACCAAC TGATTCCCAA CTTGTACCGC TACATACAGC CATGGGAGAG TGAATTCATT 
GACTCCCAGC GGGTCTGGGC TGAGTATGCA CTCAAGCGAC AGGAGGCTAT TGCTCAGAAC 
AGACGCTTGA CTTTAGAAGA TTTAGAAGAC TCGTGGGATC GGGGCATTCC CCGGATCAAT 
ACCCTCTTCC AGAAAGATCG GCACACATTG GCTTATGATA AAGGCTGGCG TGTCCGAACT 
GACTTTAAGC AGTACCAGGT TTTGAAGCAG AACCCCTTTT GGTGGACACA TCAACGGCAT 
GATGGCAAGC TTTGGAACCT CAACAACTAC CGCACAGACA TGATCCAGGC ACTGGGCGGT 
GTGGAGGGCA TTCTGGAACA CACTCTCTTC AAGGGCACTT ACTTCCCAAC CTGGGAAGGT 
CTGTTCTGGG AGAAAGCCAG CGGGTTTGAG GAATCCATGA AGTGGAAGAA GCTGACCAAT 
GCTCAGCGAT CAGGATTAAA CCAGATTCCT AATCGTAGAT TTACTCTCTG GTGGTCCCCA 
ACAATCAATC GAGCCAATGT ATACGTAGGC TTCCAGGTGC AGCTGGACCT GACAGGAATC 
TTTATGCATG GCAAGATCCC CACGCTGAAG ATCTCTCTCA TCCAGATCTT CCGAGCTCAC 
TTGTGGCAAA AGATTCATGA GAGCATCGTG ATGGACTTGT GTCAGGTCTT CGACCAGGAA 
TTAGATGCCC TGGAAATTGA GACAGTACAA AAGGAGACAA TCCATCCCCG AAAATCCTAC 
AAGATGAACT CTTCCTGTGC AGATATACTG CTCTTTGCTT CCTATAAGTG GAATGTCTCC 
CGGCCCTCGC TGCTGGCAGA CTCTAAGGAT GTGATGGACA GCACCACCAC ACAGAAGTAT 
TGGATTGACA TCCAGTTACG CTGGGGGGAC TATGATTCCC ATGATATTGA GCGCTATGCC 
CGGGCCAAGT TCCTGGATTA CACCACAGAC AACATGAGTA TCTACCCTTC ACCCACAGGT 
GTGCTCATTG CCATTGATCT GGCCTATAAT CTACACAGTG CCTATGGAAA CTGGTTCCCG 
GGGAGCAAGC CTCTCATACA GCAAGCCATG GCCAAGATCA TGAAGGCAAA CCCTGCCTTG 
TATGTGTTAC GTGAACGGAT ACGCAAAGGA TTGCAGCTGT ATTCATCTGA GCCCACTGAG 
CCTTACCTGT CCTCTCAGAA CTATGGTGAA CTCTTCTCGA ACCAGATTAT CTGGTTTGTG 
GATGACACCA ATGTCTATAG AGTAACTATC CACAAGACCT TTGAGGGGAA CTTGACAACC 
AAACCCATCA ATGGGGCCAT CTTCATCTTC AATCCACGCA CAGGACAGCT GTTCCTCAAG 
ATAATCCACA CATCCGTATG GGCTGGACAG AAGCGTTTGG GTCAGTTGGC CAAGTGGAAG 
ACTGCTGAAG AAGTGGCTGC CCTGATCCGA TCTCTGCCTG TGGAGGAACA GCCTAAACAG 
ATTATTGTCA CTCGGAAAGG GATGCTGGAT CCCCTGGAGG TACACTTACT GGACTTCCCC 
AATATTGTGA TCAAAGGATC AGAGCTCCAG CTGCCCTTCC AGGCTTGTCT CAAGGTGGAG 
AAGTTTGGGG ATCTCATCCT TAAAGCCACA GAACCACAGA TGGTTCTCTT CAACCTCTAT 
GATGACTGGC TCAAGACTAT CTCATCCTAC ACGGCTTTCT CCCGTCTCAT CCTGATCCTG 
CGTGCTTTGC ATGTGAACAA CGATAGGGCA AAAGTGATCC TGAAGCCAGA CAAGACAACC 
GTTACGGAAC CCCACCACAT CTGGCCCACG CTCACTGACG AGGAGTGGAT CAAGGTGGAG 
GTGCAGCTCA AGGATCTGAT CTTGGCTGAC TATGGCAAGA AAAACAATGT GAATGTGGCA 
TCGCTGACAC AATCAGAAAT TCGAGACATC ATCCTGGGAA TGGAGATCTC TGCACCATCG 
CAGCAGCGGC AGCAGATAGC TGAGATTGAG AAGCAGACCA AGGAACAGTC CCAGCTCACT 
GCTACACAGA CTCGAACTGT CAACAAGCAT GGCGATGAGA TCATCACCTC CACCACTAGC 
AACTATGAGA CCCAGACCTT CTCGTCGAAG ACCGAATGGA GAGTCAGGGC CATCTCTGCT 
GCCAACCTGC ATCTACGGAC CAATCACATT TATGTTTCAT CTGATGACAT CAAGGAGACT 
GGTTATACGT ACATTCTTCC CAAAAATGTG CTGAAGAAGT TCATCTGCAT ATCTGATCTG 
CGCGCCCAAA TTGCAGGATA CCTATATGGG GTGAGCCCTC CAGATAACCC TCAGGTGAAG 
GAGATCCGCT GTATTGTGAT GGTGCCACAG TGGGGCACTC ACCAGACTGT GCACCTGCCT 
AGTCAGCTGC CTCAACATGA GTACCTCAAG GAGATGGAAC CCTTAGGTTG GATCCACACT 
CAACCCAATG AATCCCCCCA GCTATCGCCC CAAGATGTAA CTACTCATGC CAAAATCATG 
GCTGACAACC CATCTTGGGA TGGGGAGAAG ACTATTATCA TCACCTGCAG CTTCACACCA 
GGTTCCTGCA CACTGACAGC CTACAAGCTG ACCCCTAGCG GGTATGAGTG GGGTCGGCAG 
AACACAGACA AAGGCAACAA CCCCAAAGGC TACCTACCTT CGCACTATGA GAGGGTTCAG 
ATGCTCCTGT CTGACCGATT CCTCGGCTTC TTTATGGTCC CTGCCCAGTC CTCCTGGAAC 
TACAACTTTA TGGGTGTTCG ACATGACCCC AACATGAAAT ACGAGCTGCA GCTAGCAAAC 
CCCAAGGAGT TCTACCATGA GGTCCACAGG CCCTCCCACT TCCTCAACTT TGCCCTCCTG 
CAGGAGGGTG AGGTCTATTC CGCAGACAGA GAGGACCTCT ATGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006246954.1
CDS	285..7292
Translation	MAGVFPYRGPGNPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKEDMPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRKYVNGSTYQRWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPISHRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRRALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCWKANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLTRLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLILALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWETSEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATEAAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKCRTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTVTEPHHIWPTLTDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPSQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWNYNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA

Target ORF information:

RefSeq Version	XM_006246892.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus pre-mRNA processing factor 8 (Prpf8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006246892.3

ORF Insert Sequence:

ATGGCCGGAG TGTTTCCTTA CAGAGGGCCG GGTAACCCGG TGCCCGGCCC TCTAGCCCCG 
CTGCCGGATT ACATGTCAGA GGAGAAGCTG CAGGAGAAAG CTCGAAAATG GCAGCAGTTG 
CAGGCCAAGC GCTATGCAGA GAAGCGGAAA TTCGGGTTTG TGGATGCTCA GAAGGAAGAC 
ATGCCCCCAG AACATGTCAG GAAAATCATT CGAGACCATG GAGACATGAC CAACAGGAAG 
TTCCGTCATG ACAAAAGGGT CTATTTGGGA GCTCTCAAGT ACATGCCTCA CGCAGTTCTC 
AAGCTCCTAG AGAACATGCC CATGCCTTGG GAGCAGATCC GGGATGTGCC TGTGCTGTAC 
CACATCACTG GAGCCATTTC CTTCGTTAAC GAGATTCCCT GGGTTATTGA GCCAGTTTAC 
ATCTCCCAGT GGGGGTCAAT GTGGATCATG ATGCGTAGAG AAAAAAGAGA CAGGAGGCAT 
TTCAAGAGGA TGCGTTTTCC CCCTTTTGAT GATGAGGAGC CGCCCCTGGA TTATGCTGAC 
AACATCTTAG ATGTCGAGCC ACTGGAAGCC ATTCAGCTAG AGCTGGACCC TGAGGAGGAT 
GCCCCTGTGT TGGACTGGTT CTATGACCAC CAGCCATTGA GAGATAGTAG GAAGTATGTT 
AATGGTTCCA CCTACCAACG CTGGCAGTTC ACATTGCCTA TGATGTCCAC TCTCTACCGA 
CTGGCCAACC AGCTTCTGAC TGACTTGGTA GATGACAACT ACTTTTATCT CTTTGATCTA 
AAGGCCTTCT TTACCTCTAA GGCACTCAAC ATGGCCATTC CTGGGGGACC CAAATTTGAA 
CCTCTTGTTC GAGACATCAA CCTACAGGAT GAAGACTGGA ATGAATTCAA TGATATTAAC 
AAGATCATCA TCCGTCAGCC TATTCGTACT GAGTACAAGA TTGCTTTTCC GTACCTCTAT 
AACAACCTCC CACACCATGT CCACCTGACC TGGTACCACA CTCCTAACGT TGTGTTCATC 
AAGACTGAGG ATCCTGATCT GCCAGCTTTC TACTTTGATC CTCTGATTAA CCCAATCTCC 
CATAGACACT CAGTCAAGAG CCAAGAGCCA CTACCTGATG ATGATGAGGA GTTTGAGCTC 
CCAGAGTTTG TGGAGCCCTT CCTGAAGGAT ACACCTCTCT ATACAGACAA TACAGCCAAT 
GGCATCGCAC TGCTCTGGGC ACCACGGCCC TTCAACCTAC GCTCTGGTCG TACCCGCCGG 
GCCCTCGACA TTCCCCTTGT CAAGAACTGG TATCGGGAGC ACTGTCCTGC TGGGCAACCT 
GTGAAAGTTC GAGTCTCCTA TCAGAAGCTG CTTAAGTATT ATGTGCTGAA TGCCCTCAAA 
CATCGGCCTC CCAAAGCGCA GAAGAAGAGG TATCTGTTTC GCTCTTTCAA AGCTACCAAA 
TTCTTTCAGT CTACCAAGCT GGACTGGGTG GAGGTCGGGC TGCAGGTTTG CCGCCAGGGC 
TACAACATGC TTAACCTTCT CATTCACCGT AAGAACCTCA ACTATCTGCA TTTGGACTAT 
AACTTCAACC TGAAGCCTGT CAAGACGCTC ACCACCAAGG AAAGAAAAAA ATCTCGCTTT 
GGGAATGCTT TCCATCTGTG TCGGGAAGTA CTGCGTTTGA CTAAGCTGGT TGTGGATAGT 
CATGTGCAGT ATCGCTTGGG CAATGTAGAT GCCTTCCAGC TGGCAGATGG ATTGCAGTAT 
ATATTTGCCC ACGTGGGACA GTTGACAGGC ATGTATCGAT ACAAATATAA GCTGATGCGG 
CAGATCCGCA TGTGCAAGGA CTTGAAACAT CTCATCTATT ACCGCTTCAA CACGGGTCCT 
GTGGGGAAAG GCCCTGGCTG TGGTTTCTGG GCTGCTGGCT GGCGGGTCTG GCTATTTTTT 
ATGCGTGGAA TTACCCCTTT GCTAGAACGA TGGCTAGGCA ACCTTCTGGC CCGACAGTTT 
GAAGGCCGTC ACTCAAAGGG GGTGGCAAAG ACAGTAACAA AGCAACGAGT AGAGTCCCAT 
TTTGACCTTG AGCTTAGGGC AGCTGTGATG CATGATATTC TGGACATGAT GCCTGAAGGC 
ATCAAACAGA ACAAGGCCCG AACAATTCTG CAGCACCTCA GTGAGGCCTG GCGCTGCTGG 
AAGGCCAACA TTCCATGGAA GGTCCCAGGA CTGCCAACGC CTATAGAGAA TATGATTCTT 
CGGTACGTTA AGGCCAAGGC TGATTGGTGG ACCAATACTG CCCACTACAA CCGTGAACGG 
ATTCGCCGTG GGGCTACTGT AGACAAGACT GTTTGCAAAA AGAACCTGGG CCGCCTCACC 
CGCCTGTATC TGAAGGCAGA ACAGGAGCGG CAGCACAATT ACCTGAAGGA CGGGCCTTAC 
ATCACAGCAG AGGAAGCAGT GGCAGTGTAT ACTACCACTG TGCACTGGCT GGAAAGCCGT 
AGGTTCTCTC CTATCCCGTT CCCCCCACTC TCCTACAAGC ATGACACCAA GTTGCTTATC 
TTGGCTCTGG AGCGACTCAA GGAAGCTTAT AGTGTGAAGT CTAGGTTAAA CCAGTCTCAG 
AGGGAGGAGC TGGGTCTGAT TGAACAAGCC TATGACAACC CCCATGAGGC TTTGTCCCGC 
ATCAAGCGTC ACCTCCTCAC GCAGAGAGCC TTTAAGGAAG TGGGCATTGA GTTTATGGAT 
CTCTATAGCC ACCTTGTGCC AGTGTATGAT GTGGAACCAC TGGAGAAAAT AACTGATGCT 
TACCTGGACC AGTACCTGTG GTATGAAGCA GATAAACGCC GTCTTTTCCC GCCCTGGATC 
AAGCCTGCTG ACACAGAACC CCCTCCACTG CTTGTTTACA AGTGGTGCCA AGGCATCAAT 
AACCTGCAGG ATGTGTGGGA GACGAGTGAG GGTGAGTGCA ATGTCATGCT GGAGTCTCGG 
TTTGAGAAGA TGTATGAGAA GATTGACCTG ACCCTGCTCA ACAGGCTGCT GCGACTCATT 
GTGGACCACA ACATAGCTGA CTACATGACA GCCAAGAACA ATGTAGTCAT CAACTATAAG 
GACATGAACC ATACAAATTC ATATGGAATC ATCAGAGGCC TGCAGTTTGC CTCATTCATT 
GTACAGTACT ATGGTTTGGT GATGGATTTG CTTGTATTGG GATTGCACCG GGCCAGTGAG 
ATGGCTGGGC CTCCGCAGAT GCCAAATGAC TTTCTCAGTT TTCAGGACAT AGCCACCGAG 
GCTGCACACC CAATCCGACT CTTCTGTAGA TACATTGATC GCATCCATAT TTTCTTCAGG 
TTCACAGCAG ATGAGGCTCG GGATCTGATC CAGCGTTACC TGACAGAACA TCCAGACCCC 
AATAATGAAA ACATTGTTGG CTACAATAAT AAGAAATGCT GGCCCCGAGA TGCCCGTATG 
CGTCTCATGA AGCATGATGT CAACTTGGGT CGGGCAGTGT TCTGGGACAT CAAAAACCGG 
CTGCCACGAT CGGTGACTAC AGTACAGTGG GAGAATAGCT TTGTGTCTGT GTACAGTAAA 
GACAACCCCA ACCTGCTGTT CAACATGTGT GGCTTTGAGT GCCGCATCCT ACCTAAGTGC 
CGTACCAGCT ATGAAGAGTT CACCCACAAG GACGGGGTCT GGAATCTACA GAATGAGGTT 
ACTAAGGAGC GGACGGCTCA GTGTTTCCTG CGTGTGGACG ATGAGTCAAT GCAGCGCTTC 
CATAACCGCG TGCGGCAGAT TCTGATGGCC TCTGGCTCTA CCACTTTCAC CAAGATTGTG 
AATAAGTGGA ATACAGCTCT CATTGGCCTT ATGACATACT TTCGAGAGGC TGTGGTGAAT 
ACACAGGAAC TCCTAGACTT ACTGGTGAAG TGTGAAAACA AGATCCAGAC ACGTATCAAG 
ATTGGATTGA ACTCCAAGAT GCCAAGTCGG TTCCCCCCTG TTGTATTCTA CACCCCTAAG 
GAGCTGGGTG GGCTTGGCAT GCTCTCGATG GGCCATGTCC TTATCCCCCA GTCTGACCTC 
AGGTGGTCCA AGCAAACAGA TGTGGGCATC ACACACTTTC GTTCAGGAAT GAGTCATGAA 
GAAGACCAAC TGATTCCCAA CTTGTACCGC TACATACAGC CATGGGAGAG TGAATTCATT 
GACTCCCAGC GGGTCTGGGC TGAGTATGCA CTCAAGCGAC AGGAGGCTAT TGCTCAGAAC 
AGACGCTTGA CTTTAGAAGA TTTAGAAGAC TCGTGGGATC GGGGCATTCC CCGGATCAAT 
ACCCTCTTCC AGAAAGATCG GCACACATTG GCTTATGATA AAGGCTGGCG TGTCCGAACT 
GACTTTAAGC AGTACCAGGT TTTGAAGCAG AACCCCTTTT GGTGGACACA TCAACGGCAT 
GATGGCAAGC TTTGGAACCT CAACAACTAC CGCACAGACA TGATCCAGGC ACTGGGCGGT 
GTGGAGGGCA TTCTGGAACA CACTCTCTTC AAGGGCACTT ACTTCCCAAC CTGGGAAGGT 
CTGTTCTGGG AGAAAGCCAG CGGGTTTGAG GAATCCATGA AGTGGAAGAA GCTGACCAAT 
GCTCAGCGAT CAGGATTAAA CCAGATTCCT AATCGTAGAT TTACTCTCTG GTGGTCCCCA 
ACAATCAATC GAGCCAATGT ATACGTAGGC TTCCAGGTGC AGCTGGACCT GACAGGAATC 
TTTATGCATG GCAAGATCCC CACGCTGAAG ATCTCTCTCA TCCAGATCTT CCGAGCTCAC 
TTGTGGCAAA AGATTCATGA GAGCATCGTG ATGGACTTGT GTCAGGTCTT CGACCAGGAA 
TTAGATGCCC TGGAAATTGA GACAGTACAA AAGGAGACAA TCCATCCCCG AAAATCCTAC 
AAGATGAACT CTTCCTGTGC AGATATACTG CTCTTTGCTT CCTATAAGTG GAATGTCTCC 
CGGCCCTCGC TGCTGGCAGA CTCTAAGGAT GTGATGGACA GCACCACCAC ACAGAAGTAT 
TGGATTGACA TCCAGTTACG CTGGGGGGAC TATGATTCCC ATGATATTGA GCGCTATGCC 
CGGGCCAAGT TCCTGGATTA CACCACAGAC AACATGAGTA TCTACCCTTC ACCCACAGGT 
GTGCTCATTG CCATTGATCT GGCCTATAAT CTACACAGTG CCTATGGAAA CTGGTTCCCG 
GGGAGCAAGC CTCTCATACA GCAAGCCATG GCCAAGATCA TGAAGGCAAA CCCTGCCTTG 
TATGTGTTAC GTGAACGGAT ACGCAAAGGA TTGCAGCTGT ATTCATCTGA GCCCACTGAG 
CCTTACCTGT CCTCTCAGAA CTATGGTGAA CTCTTCTCGA ACCAGATTAT CTGGTTTGTG 
GATGACACCA ATGTCTATAG AGTAACTATC CACAAGACCT TTGAGGGGAA CTTGACAACC 
AAACCCATCA ATGGGGCCAT CTTCATCTTC AATCCACGCA CAGGACAGCT GTTCCTCAAG 
ATAATCCACA CATCCGTATG GGCTGGACAG AAGCGTTTGG GTCAGTTGGC CAAGTGGAAG 
ACTGCTGAAG AAGTGGCTGC CCTGATCCGA TCTCTGCCTG TGGAGGAACA GCCTAAACAG 
ATTATTGTCA CTCGGAAAGG GATGCTGGAT CCCCTGGAGG TACACTTACT GGACTTCCCC 
AATATTGTGA TCAAAGGATC AGAGCTCCAG CTGCCCTTCC AGGCTTGTCT CAAGGTGGAG 
AAGTTTGGGG ATCTCATCCT TAAAGCCACA GAACCACAGA TGGTTCTCTT CAACCTCTAT 
GATGACTGGC TCAAGACTAT CTCATCCTAC ACGGCTTTCT CCCGTCTCAT CCTGATCCTG 
CGTGCTTTGC ATGTGAACAA CGATAGGGCA AAAGTGATCC TGAAGCCAGA CAAGACAACC 
GTTACGGAAC CCCACCACAT CTGGCCCACG CTCACTGACG AGGAGTGGAT CAAGGTGGAG 
GTGCAGCTCA AGGATCTGAT CTTGGCTGAC TATGGCAAGA AAAACAATGT GAATGTGGCA 
TCGCTGACAC AATCAGAAAT TCGAGACATC ATCCTGGGAA TGGAGATCTC TGCACCATCG 
CAGCAGCGGC AGCAGATAGC TGAGATTGAG AAGCAGACCA AGGAACAGTC CCAGCTCACT 
GCTACACAGA CTCGAACTGT CAACAAGCAT GGCGATGAGA TCATCACCTC CACCACTAGC 
AACTATGAGA CCCAGACCTT CTCGTCGAAG ACCGAATGGA GAGTCAGGGC CATCTCTGCT 
GCCAACCTGC ATCTACGGAC CAATCACATT TATGTTTCAT CTGATGACAT CAAGGAGACT 
GGTTATACGT ACATTCTTCC CAAAAATGTG CTGAAGAAGT TCATCTGCAT ATCTGATCTG 
CGCGCCCAAA TTGCAGGATA CCTATATGGG GTGAGCCCTC CAGATAACCC TCAGGTGAAG 
GAGATCCGCT GTATTGTGAT GGTGCCACAG TGGGGCACTC ACCAGACTGT GCACCTGCCT 
AGTCAGCTGC CTCAACATGA GTACCTCAAG GAGATGGAAC CCTTAGGTTG GATCCACACT 
CAACCCAATG AATCCCCCCA GCTATCGCCC CAAGATGTAA CTACTCATGC CAAAATCATG 
GCTGACAACC CATCTTGGGA TGGGGAGAAG ACTATTATCA TCACCTGCAG CTTCACACCA 
GGTTCCTGCA CACTGACAGC CTACAAGCTG ACCCCTAGCG GGTATGAGTG GGGTCGGCAG 
AACACAGACA AAGGCAACAA CCCCAAAGGC TACCTACCTT CGCACTATGA GAGGGTTCAG 
ATGCTCCTGT CTGACCGATT CCTCGGCTTC TTTATGGTCC CTGCCCAGTC CTCCTGGAAC 
TACAACTTTA TGGGTGTTCG ACATGACCCC AACATGAAAT ACGAGCTGCA GCTAGCAAAC 
CCCAAGGAGT TCTACCATGA GGTCCACAGG CCCTCCCACT TCCTCAACTT TGCCCTCCTG 
CAGGAGGGTG AGGTCTATTC CGCAGACAGA GAGGACCTCT ATGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structure of a human catalytic step I spliceosome.
Science (New York, N.Y.)(2018 Jan)
Zhan X,Yan C,Zhang X,Lei J,Shi Y

Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for Activation.
Cell170(4)701-713.e11(2017 Aug)
Bertram K,Agafonov DE,Dybkov O,Haselbach D,Leelaram MN,Will CL,Urlaub H,Kastner B,L?hrmann R,Stark H

Cryo-EM structure of a human spliceosome activated for step 2 of splicing.
Nature542(7641)318-323(2017 02)
Bertram K,Agafonov DE,Liu WT,Dybkov O,Will CL,Hartmuth K,Urlaub H,Kastner B,Stark H,L?hrmann R

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Prpf8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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