Mrpl27 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Gene Symbol	Mrpl27
Entrez Gene ID	287635
Full Name	mitochondrial ribosomal protein L27
General protein information	Preferred Names mitochondrial ribosomal protein L27 Names 39S ribosomal protein L27, mitochondrial
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q26

mRNA and Protein(s)

mRNA Protein Name

NM_001105831.1 NP_001099301.1 39S ribosomal protein L27, mitochondrial
Pathways from BioSystems

mRNA	Protein	Name
NM_001105831.1	NP_001099301.1	39S ribosomal protein L27, mitochondrial

Homologies

Pan troglodytes (chimpanzee)	MRPL27	XP_003315660.1
Rattus norvegicus (Norway rat)	Mrpl27	NP_001099301.1
Danio rerio (zebrafish)	mrpl27	NP_001018516.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002470	XP_312468.5
Gallus gallus (chicken)	MRPL27	XP_001232140.1
Drosophila melanogaster (fruit fly)	mRpL27	NP_001260028.1
Canis lupus familiaris (dog)	MRPL27	XP_548198.3
Xenopus tropicalis (tropical clawed frog)	mrpl27	NP_001016630.1
Homo sapiens (human)	MRPL27	NP_057588.1
Bos taurus (cattle)	MRPL27	NP_001032530.1
Mus musculus (house mouse)	Mrpl27	NP_444391.1

Gene Ontology
Bibliography

Related articles in PubMed

Structures of the human mitochondrial ribosome in native states of assembly.
Brown A, Rathore S, Kimanius D, Aibara S, Bai XC, Rorbach J, Amunts A, Ramakrishnan V
Nature structural & molecular biology24(10)866-869(2017 Oct)

Structure of the large ribosomal subunit from human mitochondria.
Brown A, Amunts A, Bai XC, Sugimoto Y, Edwards PC, Murshudov G, Scheres SHW, Ramakrishnan V
Science (New York, N.Y.)346(6210)718-722(2014 Nov)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Mootha VK, Bunkenborg J, Olsen JV, Hjerrild M, Wisniewski JR, Stahl E, Bolouri MS, Ray HN, Sihag S, Kamal M, Patterson N, Lander ES, Mann M
Cell115(5)629-40(2003 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Mrpl27 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mrpl27 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa11489	NM_001105831.1 Latest version!	Rattus norvegicus mitochondrial ribosomal protein L27 (Mrpl27), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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1 Clone	30% OFF
2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mrpl27 ( NM_001105831.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099301.1 Rattus norvegicus mitochondrial ribosomal protein L27 (Mrpl27), mRNA.

Price & Availability↑

CloneID	ORa11489
Clone ID Related Accession (Same CDS sequence)	NM_001105831.1
Accession Version	NM_001105831.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	39S ribosomal protein L27, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473948.1. On or before Oct 3, 2007 this sequence version replaced XM_213439.4, XM_001081298.1. ##Evidence-Data-START## Transcript exon combination :: FM054246.1, FM073931.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCGTCGG CGGCGGCGGC CCTTACGCTG AGGACGCGGG CAGCAGCCCT GCTGAGTCCC 
ACAGCACCTA CAGCACTTGC TGTCAGATCC GCATCCAAGA AGACAGGTGG CAGTTCTAAG 
AACCTTGGTG GCAAATCACG AGGCAAACAC TATGGCATCA AGAAAATGGA AGGTCACTAC 
GTTCATGCCG GCAACATCCT TGGCACTCAG CGGCAGTTCA GATGGCACCC AGGCGCCCAT 
GTGGGACTGG GGAAGAACAA GTGCCTGTAT GCCCTGGAGG AGGGGATAGT TCGCTACACG 
AAAGAAGTCT ACGTGCCCAA TCCCAAAAAC TCGGAGGCTG TGAATCTGGT CACTAGTCTG 
CCCAAGGGTG CTGTGCTCTA CAAGACTTTT GTCCACGTGG TTCCTGCCAA ACCGGAGGGA 
ACCTTCAAAC TGGTAGACAT GCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099301.1
CDS	6..452
Translation	MASAAAALTLRTRAAALLSPTAPTALAVRSASKKTGGSSKNLGGKSRGKHYGIKKMEGHYVHAGNILGTQRQFRWHPGAHVGLGKNKCLYALEEGIVRYTKEVYVPNPKNSEAVNLVTSLPKGAVLYKTFVHVVPAKPEGTFKLVDML

Target ORF information:

RefSeq Version	NM_001105831.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitochondrial ribosomal protein L27 (Mrpl27), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001105831.1

ORF Insert Sequence:

ATGGCGTCGG CGGCGGCGGC CCTTACGCTG AGGACGCGGG CAGCAGCCCT GCTGAGTCCC 
ACAGCACCTA CAGCACTTGC TGTCAGATCC GCATCCAAGA AGACAGGTGG CAGTTCTAAG 
AACCTTGGTG GCAAATCACG AGGCAAACAC TATGGCATCA AGAAAATGGA AGGTCACTAC 
GTTCATGCCG GCAACATCCT TGGCACTCAG CGGCAGTTCA GATGGCACCC AGGCGCCCAT 
GTGGGACTGG GGAAGAACAA GTGCCTGTAT GCCCTGGAGG AGGGGATAGT TCGCTACACG 
AAAGAAGTCT ACGTGCCCAA TCCCAAAAAC TCGGAGGCTG TGAATCTGGT CACTAGTCTG 
CCCAAGGGTG CTGTGCTCTA CAAGACTTTT GTCCACGTGG TTCCTGCCAA ACCGGAGGGA 
ACCTTCAAAC TGGTAGACAT GCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structures of the human mitochondrial ribosome in native states of assembly.
Nature structural & molecular biology24(10)866-869(2017 Oct)
Brown A,Rathore S,Kimanius D,Aibara S,Bai XC,Rorbach J,Amunts A,Ramakrishnan V

Structure of the large ribosomal subunit from human mitochondria.
Science (New York, N.Y.)346(6210)718-722(2014 Nov)
Brown A,Amunts A,Bai XC,Sugimoto Y,Edwards PC,Murshudov G,Scheres SHW,Ramakrishnan V

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Cell115(5)629-40(2003 Nov)
Mootha VK,Bunkenborg J,Olsen JV,Hjerrild M,Wisniewski JR,Stahl E,Bolouri MS,Ray HN,Sihag S,Kamal M,Patterson N,Lander ES,Mann M

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Mrpl27 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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