• English
  • Sign In
    Please Sign In
    Sign In
    New to GenScript?
    Register
  • Contact Us
×
Contact us now: +1-877-436-7274

Usp14 cDNA ORF clone, Rattus norvegicus(Norway rat)

Summary

Gene Symbol Usp14
Entrez Gene ID 291796
Full Name ubiquitin specific peptidase 14
General protein information
Preferred Names

ubiquitin specific peptidase 14

Names

ubiquitin carboxyl-terminal hydrolase 14
ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
ubiquitin specific protease 14
Gene Type protein-coding
Organism Rattus norvegicus(Norway rat)
Genome

18

18p13

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001008301.1 NP_001008302.1 ubiquitin carboxyl-terminal hydrolase 14
XM_006254398.3 XP_006254460.1 ubiquitin carboxyl-terminal hydrolase 14 isoform X2
XM_006254396.2 XP_006254458.1 ubiquitin carboxyl-terminal hydrolase 14 isoform X1

Pathways from BioSystems


Homologies


Homo sapiens (human) USP14 NP_005142.1
Canis lupus familiaris (dog) USP14 XP_537306.2
Gallus gallus (chicken) USP14 XP_004939812.1
Xenopus tropicalis (tropical clawed frog) usp14 NP_001005641.1
Bos taurus (cattle) USP14 NP_001068657.1
Caenorhabditis elegans (roundworm) usp-14 NP_497006.1
Schizosaccharomyces pombe (fission yeast) ubp6 NP_594117.2
Pan troglodytes (chimpanzee) USP14 NP_001038961.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP009348 XP_310014.4
Magnaporthe oryzae (rice blast fungus) MGG_02970 XP_003720730.1
Neurospora crassa NCU01919 XP_965550.1
Drosophila melanogaster (fruit fly) CG5384 NP_001260321.1
Saccharomyces cerevisiae (baker's yeast) UBP6 NP_116665.1
Kluyveromyces lactis KLLA0A10791g XP_451470.1
Eremothecium gossypii AGOS_AEL029W NP_984832.2
Arabidopsis thaliana (thale cress) UBP7 NP_566680.2
Oryza sativa (rice) Os01g0550100 NP_001043299.1
USP14 XP_001090784.1
Mus musculus (house mouse) Usp14 NP_067497.2
Rattus norvegicus (Norway rat) Usp14 NP_001008302.1
Danio rerio (zebrafish) usp14 NP_956267.1
Arabidopsis thaliana (thale cress) UBP6 NP_564596.1

Gene Ontology


Bibliography

Related articles in PubMed

Neurotoxic mechanisms by which the USP14 inhibitor IU1 depletes ubiquitinated proteins and Tau in rat cerebral cortical neurons: Relevance to Alzheimer's disease.
Kiprowska MJ, Stepanova A, Todaro DR, Galkin A, Haas A, Wilson SM, Figueiredo-Pereira ME
Biochimica et biophysica acta1863(6)1157-1170(2017 Jun)

Ubiquitin-specific protease 14 regulates cardiac hypertrophy progression by increasing GSK-3? phosphorylation.
Liu N, Chai R, Liu B, Zhang Z, Zhang S, Zhang J, Liao Y, Cai J, Xia X, Li A, Liu J, Huang H, Liu S
Biochemical and biophysical research communications478(3)1236-41(2016 09)

Inhibition of deubiquitinating activity of USP14 decreases tyrosine hydroxylase phosphorylated at Ser19 in PC12D cells.
Nakashima A, Ohnuma S, Kodani Y, Kaneko YS, Nagasaki H, Nagatsu T, Ota A
Biochemical and biophysical research communications472(4)598-602(2016 Apr)

Deubiquitination of Dishevelled by Usp14 is required for Wnt signaling.
Jung H, Kim BG, Han WH, Lee JH, Cho JY, Park WS, Maurice MM, Han JK, Lee MJ, Finley D, Jho EH
Oncogenesis2e64(2013 Aug)

USP14 inhibits ER-associated degradation via interaction with IRE1alpha.
Nagai A, Kadowaki H, Maruyama T, Takeda K, Nishitoh H, Ichijo H
Biochemical and biophysical research communications379(4)995-1000(2009 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

mediates the development of cardiac hypertrophy by promoting GSK-3beta phosphorylation.
Title: Ubiquitin-specific protease 14 regulates cardiac hypertrophy progression by increasing GSK-3? phosphorylation.

Activation of proteasome degradation by IU-1, which is an inhibitor for the deubiquitinating activity of USP14, decreased only the quantity of TH molecules phosphorylated at their Ser19.
Title: Inhibition of deubiquitinating activity of USP14 decreases tyrosine hydroxylase phosphorylated at Ser19 in PC12D cells.

The following Usp14 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Usp14 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa11224 NM_001008301.1
Latest version!		 Rattus norvegicus ubiquitin specific peptidase 14 (Usp14), mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $223.30
$319.00
ORa11224 XM_006254396.2
Latest version!		 Rattus norvegicus ubiquitin specific peptidase 14 (Usp14), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $223.30
$319.00
ORa38167 XM_006254398.3
Latest version!		 Rattus norvegicus ubiquitin specific peptidase 14 (Usp14), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID ORa11224
Clone ID Related Accession (Same CDS sequence) NM_001008301.1 , XM_006254396.2
Accession Version NM_001008301.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1482bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-12-01
Organism Rattus norvegicus(Norway rat)
Product ubiquitin carboxyl-terminal hydrolase 14
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC085947.1. On Dec 16, 2004 this sequence version replaced XM_214624.2. ##Evidence-Data-START## Transcript exon combination :: BC085947.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGCCACTCT ACTCTGTTAC AGTAAAATGG GGAAAGGAGA AATTTGAAGG TGTAGAATTG 
AATACTGATG AACCTCCAAT GGTGTTCAAG GCCCAGCTTT TTGCATTGAC TGGAGTCCAG 
CCAGCCAGAC AAAAAGTTAT GGTGAAAGGA GGAACCCTGA AGGATGATGA CTGGGGAAAC 
ATCAAAATGA AAAATGGAAT GACTGTACTA ATGATGGGGT CAGCAGATGC TCTTCCAGAA 
GAACCCTCTG CTAAAACTGT CTTCGTAGAA GACATGACAG AAGAACAGTT AGCAACTGCT 
ATGGAGTTGC CGTGTGGATT GACAAACCTC GGTAACACTT GTTACATGAA CGCTACAGTT 
CAGTGTATTC GCTCTGTGCC TGAACTCAAA GACGCCCTTA AAAGGTATGC AGGTGCCTTG 
AGAGCTTCAG GGGAAATGGC TTCAGCACAG TATATTACTG CAGCTCTTAG AGATTTGTTT 
GATTCCATGG ATAAAACTTC TTCTAGTATC CCACCTATTA TTCTGCTGCA GTTTCTGCAC 
ATGGCTTTTC CACAGTTTGC AGAGAAGGGC GAACAAGGGC AGTATCTTCA GCAGGATGCT 
AACGAGTGCT GGATACAAAT GATGAGAGTC CTGCAACAGA AACTAGAAGC CATAGAGGAT 
GATTCTGCTA GAGAGACCGA GTCTTCATCT GCATCAGCAG TCACACCTTC TAAAAAGAAA 
AGCTTAATTG ATCAGTTTTT TGGTGTTGAG TTTGAAACTA CCATGAAATG TACAGAATCT 
GAAGAAGAAG AGGTCACCAA AGGAAAGGAA AATCAACTTC AACTTAGTTG TTTCATTAAC 
CAGGAAGTGA AGTATCTTTT CACAGGGCTT AAATTGCGAC TTCAGGAAGA AATTACTAAA 
CAGTCTCCAA CATTGCAGAG AAATGCTTTG TACATCAAAT CTTCTAAGAT CAGCCGGCTA 
CCTGCTTACT TAACTATTCA GATGGTTCGA TTTTTTTATA AAGAGAAAGA ATCTGTGAAC 
GCCAAAGTTC TTAAGGATGT TAAATTTCCT CTCATGTTGG ATGTGTATGA ACTCTGTACA 
CCGGAACTTC AAGAGAAAAT GATCTCTTTT CGGTCAAAAT TCAAGGATCT AGAAGATAAA 
AAAGTGAATC AGCAACCAAA TGCAAACGAC AAGAATAGTC CTCCAAAAGA GATTAAGTAT 
GAACCCTTTT CATTTGCTGA TGATATTGGC TCCAATAATT GTGGCTACTA TGACTTACAA 
GCAGTGTTAA CTCACCAGGG AAGATCTAGT TCTTCGGGTC ATTATGTATC ATGGGTGAAA 
AGAAAAGAAG ATGAATGGAT CAAATTTGAT GACGATAAGG TCAGCATTGT AACACCAGAA 
GATATTCTGC GACTGTCTGG AGGTGGAGAC TGGCACATTG CTTATGTTCT ACTTTATGGG 
CCTCGAAGGG TTGAAATAAT GGAAGAAGAC AGTGAACAGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001008302.1
CDS123..1604
Translation

Target ORF information:

RefSeq Version NM_001008301.1
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus ubiquitin specific peptidase 14 (Usp14), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001008301.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGCCACTCT ACTCTGTTAC AGTAAAATGG GGAAAGGAGA AATTTGAAGG TGTAGAATTG 
AATACTGATG AACCTCCAAT GGTGTTCAAG GCCCAGCTTT TTGCATTGAC TGGAGTCCAG 
CCAGCCAGAC AAAAAGTTAT GGTGAAAGGA GGAACCCTGA AGGATGATGA CTGGGGAAAC 
ATCAAAATGA AAAATGGAAT GACTGTACTA ATGATGGGGT CAGCAGATGC TCTTCCAGAA 
GAACCCTCTG CTAAAACTGT CTTCGTAGAA GACATGACAG AAGAACAGTT AGCAACTGCT 
ATGGAGTTGC CGTGTGGATT GACAAACCTC GGTAACACTT GTTACATGAA CGCTACAGTT 
CAGTGTATTC GCTCTGTGCC TGAACTCAAA GACGCCCTTA AAAGGTATGC AGGTGCCTTG 
AGAGCTTCAG GGGAAATGGC TTCAGCACAG TATATTACTG CAGCTCTTAG AGATTTGTTT 
GATTCCATGG ATAAAACTTC TTCTAGTATC CCACCTATTA TTCTGCTGCA GTTTCTGCAC 
ATGGCTTTTC CACAGTTTGC AGAGAAGGGC GAACAAGGGC AGTATCTTCA GCAGGATGCT 
AACGAGTGCT GGATACAAAT GATGAGAGTC CTGCAACAGA AACTAGAAGC CATAGAGGAT 
GATTCTGCTA GAGAGACCGA GTCTTCATCT GCATCAGCAG TCACACCTTC TAAAAAGAAA 
AGCTTAATTG ATCAGTTTTT TGGTGTTGAG TTTGAAACTA CCATGAAATG TACAGAATCT 
GAAGAAGAAG AGGTCACCAA AGGAAAGGAA AATCAACTTC AACTTAGTTG TTTCATTAAC 
CAGGAAGTGA AGTATCTTTT CACAGGGCTT AAATTGCGAC TTCAGGAAGA AATTACTAAA 
CAGTCTCCAA CATTGCAGAG AAATGCTTTG TACATCAAAT CTTCTAAGAT CAGCCGGCTA 
CCTGCTTACT TAACTATTCA GATGGTTCGA TTTTTTTATA AAGAGAAAGA ATCTGTGAAC 
GCCAAAGTTC TTAAGGATGT TAAATTTCCT CTCATGTTGG ATGTGTATGA ACTCTGTACA 
CCGGAACTTC AAGAGAAAAT GATCTCTTTT CGGTCAAAAT TCAAGGATCT AGAAGATAAA 
AAAGTGAATC AGCAACCAAA TGCAAACGAC AAGAATAGTC CTCCAAAAGA GATTAAGTAT 
GAACCCTTTT CATTTGCTGA TGATATTGGC TCCAATAATT GTGGCTACTA TGACTTACAA 
GCAGTGTTAA CTCACCAGGG AAGATCTAGT TCTTCGGGTC ATTATGTATC ATGGGTGAAA 
AGAAAAGAAG ATGAATGGAT CAAATTTGAT GACGATAAGG TCAGCATTGT AACACCAGAA 
GATATTCTGC GACTGTCTGG AGGTGGAGAC TGGCACATTG CTTATGTTCT ACTTTATGGG 
CCTCGAAGGG TTGAAATAAT GGAAGAAGAC AGTGAACAGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa11224
Clone ID Related Accession (Same CDS sequence) NM_001008301.1 , XM_006254396.2
Accession Version XM_006254396.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1482bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product ubiquitin carboxyl-terminal hydrolase 14 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005117.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006254396.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGCCACTCT ACTCTGTTAC AGTAAAATGG GGAAAGGAGA AATTTGAAGG TGTAGAATTG 
AATACTGATG AACCTCCAAT GGTGTTCAAG GCCCAGCTTT TTGCATTGAC TGGAGTCCAG 
CCAGCCAGAC AAAAAGTTAT GGTGAAAGGA GGAACCCTGA AGGATGATGA CTGGGGAAAC 
ATCAAAATGA AAAATGGAAT GACTGTACTA ATGATGGGGT CAGCAGATGC TCTTCCAGAA 
GAACCCTCTG CTAAAACTGT CTTCGTAGAA GACATGACAG AAGAACAGTT AGCAACTGCT 
ATGGAGTTGC CGTGTGGATT GACAAACCTC GGTAACACTT GTTACATGAA CGCTACAGTT 
CAGTGTATTC GCTCTGTGCC TGAACTCAAA GACGCCCTTA AAAGGTATGC AGGTGCCTTG 
AGAGCTTCAG GGGAAATGGC TTCAGCACAG TATATTACTG CAGCTCTTAG AGATTTGTTT 
GATTCCATGG ATAAAACTTC TTCTAGTATC CCACCTATTA TTCTGCTGCA GTTTCTGCAC 
ATGGCTTTTC CACAGTTTGC AGAGAAGGGC GAACAAGGGC AGTATCTTCA GCAGGATGCT 
AACGAGTGCT GGATACAAAT GATGAGAGTC CTGCAACAGA AACTAGAAGC CATAGAGGAT 
GATTCTGCTA GAGAGACCGA GTCTTCATCT GCATCAGCAG TCACACCTTC TAAAAAGAAA 
AGCTTAATTG ATCAGTTTTT TGGTGTTGAG TTTGAAACTA CCATGAAATG TACAGAATCT 
GAAGAAGAAG AGGTCACCAA AGGAAAGGAA AATCAACTTC AACTTAGTTG TTTCATTAAC 
CAGGAAGTGA AGTATCTTTT CACAGGGCTT AAATTGCGAC TTCAGGAAGA AATTACTAAA 
CAGTCTCCAA CATTGCAGAG AAATGCTTTG TACATCAAAT CTTCTAAGAT CAGCCGGCTA 
CCTGCTTACT TAACTATTCA GATGGTTCGA TTTTTTTATA AAGAGAAAGA ATCTGTGAAC 
GCCAAAGTTC TTAAGGATGT TAAATTTCCT CTCATGTTGG ATGTGTATGA ACTCTGTACA 
CCGGAACTTC AAGAGAAAAT GATCTCTTTT CGGTCAAAAT TCAAGGATCT AGAAGATAAA 
AAAGTGAATC AGCAACCAAA TGCAAACGAC AAGAATAGTC CTCCAAAAGA GATTAAGTAT 
GAACCCTTTT CATTTGCTGA TGATATTGGC TCCAATAATT GTGGCTACTA TGACTTACAA 
GCAGTGTTAA CTCACCAGGG AAGATCTAGT TCTTCGGGTC ATTATGTATC ATGGGTGAAA 
AGAAAAGAAG ATGAATGGAT CAAATTTGAT GACGATAAGG TCAGCATTGT AACACCAGAA 
GATATTCTGC GACTGTCTGG AGGTGGAGAC TGGCACATTG CTTATGTTCT ACTTTATGGG 
CCTCGAAGGG TTGAAATAAT GGAAGAAGAC AGTGAACAGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006254458.1
CDS317..1798
Translation

Target ORF information:

RefSeq Version XM_006254396.2
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus ubiquitin specific peptidase 14 (Usp14), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006254396.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGCCACTCT ACTCTGTTAC AGTAAAATGG GGAAAGGAGA AATTTGAAGG TGTAGAATTG 
AATACTGATG AACCTCCAAT GGTGTTCAAG GCCCAGCTTT TTGCATTGAC TGGAGTCCAG 
CCAGCCAGAC AAAAAGTTAT GGTGAAAGGA GGAACCCTGA AGGATGATGA CTGGGGAAAC 
ATCAAAATGA AAAATGGAAT GACTGTACTA ATGATGGGGT CAGCAGATGC TCTTCCAGAA 
GAACCCTCTG CTAAAACTGT CTTCGTAGAA GACATGACAG AAGAACAGTT AGCAACTGCT 
ATGGAGTTGC CGTGTGGATT GACAAACCTC GGTAACACTT GTTACATGAA CGCTACAGTT 
CAGTGTATTC GCTCTGTGCC TGAACTCAAA GACGCCCTTA AAAGGTATGC AGGTGCCTTG 
AGAGCTTCAG GGGAAATGGC TTCAGCACAG TATATTACTG CAGCTCTTAG AGATTTGTTT 
GATTCCATGG ATAAAACTTC TTCTAGTATC CCACCTATTA TTCTGCTGCA GTTTCTGCAC 
ATGGCTTTTC CACAGTTTGC AGAGAAGGGC GAACAAGGGC AGTATCTTCA GCAGGATGCT 
AACGAGTGCT GGATACAAAT GATGAGAGTC CTGCAACAGA AACTAGAAGC CATAGAGGAT 
GATTCTGCTA GAGAGACCGA GTCTTCATCT GCATCAGCAG TCACACCTTC TAAAAAGAAA 
AGCTTAATTG ATCAGTTTTT TGGTGTTGAG TTTGAAACTA CCATGAAATG TACAGAATCT 
GAAGAAGAAG AGGTCACCAA AGGAAAGGAA AATCAACTTC AACTTAGTTG TTTCATTAAC 
CAGGAAGTGA AGTATCTTTT CACAGGGCTT AAATTGCGAC TTCAGGAAGA AATTACTAAA 
CAGTCTCCAA CATTGCAGAG AAATGCTTTG TACATCAAAT CTTCTAAGAT CAGCCGGCTA 
CCTGCTTACT TAACTATTCA GATGGTTCGA TTTTTTTATA AAGAGAAAGA ATCTGTGAAC 
GCCAAAGTTC TTAAGGATGT TAAATTTCCT CTCATGTTGG ATGTGTATGA ACTCTGTACA 
CCGGAACTTC AAGAGAAAAT GATCTCTTTT CGGTCAAAAT TCAAGGATCT AGAAGATAAA 
AAAGTGAATC AGCAACCAAA TGCAAACGAC AAGAATAGTC CTCCAAAAGA GATTAAGTAT 
GAACCCTTTT CATTTGCTGA TGATATTGGC TCCAATAATT GTGGCTACTA TGACTTACAA 
GCAGTGTTAA CTCACCAGGG AAGATCTAGT TCTTCGGGTC ATTATGTATC ATGGGTGAAA 
AGAAAAGAAG ATGAATGGAT CAAATTTGAT GACGATAAGG TCAGCATTGT AACACCAGAA 
GATATTCTGC GACTGTCTGG AGGTGGAGAC TGGCACATTG CTTATGTTCT ACTTTATGGG 
CCTCGAAGGG TTGAAATAAT GGAAGAAGAC AGTGAACAGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa38167
Clone ID Related Accession (Same CDS sequence) XM_006254398.3
Accession Version XM_006254398.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1377bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product ubiquitin carboxyl-terminal hydrolase 14 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005117.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006254398.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGCCACTCT ACTCTGTTAC AGTAAAATGG GGAAAGGAGA AATTTGAAGG TGTAGAATTG 
AATACTGATG AACCTCCAAT GGTGTTCAAG GCCCAGCTTT TTGCATTGAC TGGAGTCCAG 
CCAGCCAGAC AAAAAGTTAT GGTGAAAGGA GGAACCCTGA AGGATGATGA CTGGGGAAAC 
ATCAAAATGA AAAATATGGA GTTGCCGTGT GGATTGACAA ACCTCGGTAA CACTTGTTAC 
ATGAACGCTA CAGTTCAGTG TATTCGCTCT GTGCCTGAAC TCAAAGACGC CCTTAAAAGG 
TATGCAGGTG CCTTGAGAGC TTCAGGGGAA ATGGCTTCAG CACAGTATAT TACTGCAGCT 
CTTAGAGATT TGTTTGATTC CATGGATAAA ACTTCTTCTA GTATCCCACC TATTATTCTG 
CTGCAGTTTC TGCACATGGC TTTTCCACAG TTTGCAGAGA AGGGCGAACA AGGGCAGTAT 
CTTCAGCAGG ATGCTAACGA GTGCTGGATA CAAATGATGA GAGTCCTGCA ACAGAAACTA 
GAAGCCATAG AGGATGATTC TGCTAGAGAG ACCGAGTCTT CATCTGCATC AGCAGTCACA 
CCTTCTAAAA AGAAAAGCTT AATTGATCAG TTTTTTGGTG TTGAGTTTGA AACTACCATG 
AAATGTACAG AATCTGAAGA AGAAGAGGTC ACCAAAGGAA AGGAAAATCA ACTTCAACTT 
AGTTGTTTCA TTAACCAGGA AGTGAAGTAT CTTTTCACAG GGCTTAAATT GCGACTTCAG 
GAAGAAATTA CTAAACAGTC TCCAACATTG CAGAGAAATG CTTTGTACAT CAAATCTTCT 
AAGATCAGCC GGCTACCTGC TTACTTAACT ATTCAGATGG TTCGATTTTT TTATAAAGAG 
AAAGAATCTG TGAACGCCAA AGTTCTTAAG GATGTTAAAT TTCCTCTCAT GTTGGATGTG 
TATGAACTCT GTACACCGGA ACTTCAAGAG AAAATGATCT CTTTTCGGTC AAAATTCAAG 
GATCTAGAAG ATAAAAAAGT GAATCAGCAA CCAAATGCAA ACGACAAGAA TAGTCCTCCA 
AAAGAGATTA AGTATGAACC CTTTTCATTT GCTGATGATA TTGGCTCCAA TAATTGTGGC 
TACTATGACT TACAAGCAGT GTTAACTCAC CAGGGAAGAT CTAGTTCTTC GGGTCATTAT 
GTATCATGGG TGAAAAGAAA AGAAGATGAA TGGATCAAAT TTGATGACGA TAAGGTCAGC 
ATTGTAACAC CAGAAGATAT TCTGCGACTG TCTGGAGGTG GAGACTGGCA CATTGCTTAT 
GTTCTACTTT ATGGGCCTCG AAGGGTTGAA ATAATGGAAG AAGACAGTGA ACAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006254460.1
CDS288..1664
Translation

Target ORF information:

RefSeq Version XM_006254398.3
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus ubiquitin specific peptidase 14 (Usp14), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006254398.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGCCACTCT ACTCTGTTAC AGTAAAATGG GGAAAGGAGA AATTTGAAGG TGTAGAATTG 
AATACTGATG AACCTCCAAT GGTGTTCAAG GCCCAGCTTT TTGCATTGAC TGGAGTCCAG 
CCAGCCAGAC AAAAAGTTAT GGTGAAAGGA GGAACCCTGA AGGATGATGA CTGGGGAAAC 
ATCAAAATGA AAAATATGGA GTTGCCGTGT GGATTGACAA ACCTCGGTAA CACTTGTTAC 
ATGAACGCTA CAGTTCAGTG TATTCGCTCT GTGCCTGAAC TCAAAGACGC CCTTAAAAGG 
TATGCAGGTG CCTTGAGAGC TTCAGGGGAA ATGGCTTCAG CACAGTATAT TACTGCAGCT 
CTTAGAGATT TGTTTGATTC CATGGATAAA ACTTCTTCTA GTATCCCACC TATTATTCTG 
CTGCAGTTTC TGCACATGGC TTTTCCACAG TTTGCAGAGA AGGGCGAACA AGGGCAGTAT 
CTTCAGCAGG ATGCTAACGA GTGCTGGATA CAAATGATGA GAGTCCTGCA ACAGAAACTA 
GAAGCCATAG AGGATGATTC TGCTAGAGAG ACCGAGTCTT CATCTGCATC AGCAGTCACA 
CCTTCTAAAA AGAAAAGCTT AATTGATCAG TTTTTTGGTG TTGAGTTTGA AACTACCATG 
AAATGTACAG AATCTGAAGA AGAAGAGGTC ACCAAAGGAA AGGAAAATCA ACTTCAACTT 
AGTTGTTTCA TTAACCAGGA AGTGAAGTAT CTTTTCACAG GGCTTAAATT GCGACTTCAG 
GAAGAAATTA CTAAACAGTC TCCAACATTG CAGAGAAATG CTTTGTACAT CAAATCTTCT 
AAGATCAGCC GGCTACCTGC TTACTTAACT ATTCAGATGG TTCGATTTTT TTATAAAGAG 
AAAGAATCTG TGAACGCCAA AGTTCTTAAG GATGTTAAAT TTCCTCTCAT GTTGGATGTG 
TATGAACTCT GTACACCGGA ACTTCAAGAG AAAATGATCT CTTTTCGGTC AAAATTCAAG 
GATCTAGAAG ATAAAAAAGT GAATCAGCAA CCAAATGCAA ACGACAAGAA TAGTCCTCCA 
AAAGAGATTA AGTATGAACC CTTTTCATTT GCTGATGATA TTGGCTCCAA TAATTGTGGC 
TACTATGACT TACAAGCAGT GTTAACTCAC CAGGGAAGAT CTAGTTCTTC GGGTCATTAT 
GTATCATGGG TGAAAAGAAA AGAAGATGAA TGGATCAAAT TTGATGACGA TAAGGTCAGC 
ATTGTAACAC CAGAAGATAT TCTGCGACTG TCTGGAGGTG GAGACTGGCA CATTGCTTAT 
GTTCTACTTT ATGGGCCTCG AAGGGTTGAA ATAATGGAAG AAGACAGTGA ACAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Neurotoxic mechanisms by which the USP14 inhibitor IU1 depletes ubiquitinated proteins and Tau in rat cerebral cortical neurons: Relevance to Alzheimer's disease.
Biochimica et biophysica acta1863(6)1157-1170(2017 Jun)
Kiprowska MJ,Stepanova A,Todaro DR,Galkin A,Haas A,Wilson SM,Figueiredo-Pereira ME


book

Ubiquitin-specific protease 14 regulates cardiac hypertrophy progression by increasing GSK-3? phosphorylation.
Biochemical and biophysical research communications478(3)1236-41(2016 09)
Liu N,Chai R,Liu B,Zhang Z,Zhang S,Zhang J,Liao Y,Cai J,Xia X,Li A,Liu J,Huang H,Liu S


book

Inhibition of deubiquitinating activity of USP14 decreases tyrosine hydroxylase phosphorylated at Ser19 in PC12D cells.
Biochemical and biophysical research communications472(4)598-602(2016 Apr)
Nakashima A,Ohnuma S,Kodani Y,Kaneko YS,Nagasaki H,Nagatsu T,Ota A


book

Deubiquitination of Dishevelled by Usp14 is required for Wnt signaling.
Oncogenesis2e64(2013 Aug)
Jung H,Kim BG,Han WH,Lee JH,Cho JY,Park WS,Maurice MM,Han JK,Lee MJ,Finley D,Jho EH


book

USP14 inhibits ER-associated degradation via interaction with IRE1alpha.
Biochemical and biophysical research communications379(4)995-1000(2009 Feb)
Nagai A,Kadowaki H,Maruyama T,Takeda K,Nishitoh H,Ichijo H