Nebl cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nebl
Entrez Gene ID	307189
Full Name	nebulette
Synonyms	Lasp-2
General protein information	Preferred Names nebulette Names nebulette
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 17 Map Location: 17q12.3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001191694.1	NP_001178623.1	nebulette

Pathways from BioSystems

Homologies

	NEBL	XP_001095037.1
Canis lupus familiaris (dog)	NEBL	XP_849547.1
Rattus norvegicus (Norway rat)	Nebl	NP_001178623.1
Xenopus tropicalis (tropical clawed frog)	nebl	XP_002933196.2
Pan troglodytes (chimpanzee)	NEBL	XP_001154532.1
Homo sapiens (human)	NEBL	NP_006384.1
Bos taurus (cattle)	NEBL	NP_001178342.1

Gene Ontology
Bibliography

Related articles in PubMed

Nebulette mutations are associated with dilated cardiomyopathy and endocardial fibroelastosis.
Purevjav E, Varela J, Morgado M, Kearney DL, Li H, Taylor MD, Arimura T, Moncman CL, McKenna W, Murphy RT, Labeit S, Vatta M, Bowles NE, Kimura A, Boriek AM, Towbin JA
Journal of the American College of Cardiology56(18)1493-502(2010 Oct)

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)

The nebulette repeat domain is necessary for proper maintenance of tropomyosin with the cardiac sarcomere.
Bonzo JR, Norris AA, Esham M, Moncman CL
Experimental cell research314(19)3519-30(2008 Nov)

Nebulette interacts with filamin C.
Holmes WB, Moncman CL
Cell motility and the cytoskeleton65(2)130-42(2008 Feb)

Targeted disruption of nebulette protein expression alters cardiac myofibril assembly and function.
Moncman CL, Wang K
Experimental cell research273(2)204-18(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nebl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nebl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa07413	NM_001191694.1 Latest version!	Rattus norvegicus nebulette (Nebl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nebl ( NM_001191694.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001178623.1 Rattus norvegicus nebulette (Nebl), mRNA.

Price & Availability↑

CloneID	ORa07413
Clone ID Related Accession (Same CDS sequence)	NM_001191694.1
Accession Version	NM_001191694.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3048bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-31
Organism	Rattus norvegicus(Norway rat)
Product	nebulette
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AABR07028742.1. On Nov 15, 2012 this sequence version replaced XM_225644.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN02642914 [ECO:0000350] ##Evidence-Data-END##

ATGAAGGTGC CTGTATCCGG GGATGTGAAA GAGGAAACCG AAGAGGAAAA TGTGGAGGAG 
GAAGAAAAGC CAGAAGACGA GGTGTTTCTG AAACCTGTCG TTGAAGACCT AAGCATGGAG 
CTGGCCAGAA AGTGCACCGA GCTCATTAGT GACATCCATT ATAAAGAAGA CTATAGAAAG 
TCTAAGGACA AGTGTACGTC TGTGACTGAT ACCCCTACGC TAAACCATGT GAAAAATATT 
AGTGCTTTTA TTTCTGAGAC AAAATACAAA GGTACCATTA AAGCTGATCT TTCTAACTGT 
CTGTATAAGG ACATGCCTGC TACCATTGAC AGTGTCTTTG CAAGAGAAGT GTCCCAGCTT 
CAGAGTGAGG TTGCCTATAA GCAGAAGCAT GAGGCGGAGA AAGGCCTCTC CGATTATGCC 
CACATGAAGG AACCACCGGA GGTCAGACAC GCCATGGAGG TCAACAGGCA CCAGAGTAAC 
ATTTCTTACA GAAAAGATGT GCAAGGCACC CACACGTATA CAGCGGAGAT GGACAGACCG 
GATATCAAGA AGGCAACTCA GATCTCCAAG ATCATAAGCA ATGCAGAATA CAAGAAAGGA 
CAAGGCATCG TGAATAAAGA ACCTTCTGTA ATTGGGAGAC CAGATTTTGA ACATGCCGTG 
GAAGCTTCTA AACTTTCTAG TCAGGTTAAA TATAAAGAAA AGTTTGATAA TGAAATGAAG 
GGAAAAGGCC ACCATTACAA CCCTCTGGGA AGTGCGTCTT TCAGACAACA CCAGCTCGCT 
ACTGTCCTGG CAAGCGATGT GAAGTACAAG AAAGATGTTC AAACCATGCA CGAGCCAGTT 
TCAGATCTCC CAAACTTGTT GTTCTTAGAC CATGCTCTGA AAGCCAGCAG AATGCTCAGT 
GGATGGGAAT ATAAAAAGAA TTTTGAAGAA AACAAAGGAT CGTATCACTT TGATGCAGAG 
GCCCCTGAAC ACCTACACCA TAGAGGAAAT GCCACCCTCC AGAGTCAGGT AAAATACAGA 
GAAGAATATG AGAAGAATAA GGGGAAGACC ATGCTTGACT TTGTTGAGAC ACCATCATAT 
CAGTCTTCAA AGGAGGCTCA GAAGATGCAG AGCGAGAAAG TTTACAAAGA AGATTTTGAG 
AAGGAGATTA AAGGAAGATC CTCACTGGAT TTAGACAAGA CTCCTGCATT TCTACACGTG 
AAGCACATCA GCAACCTGAT GAGGGAGAAA GAATATAAAA AAGATTTGGA AAATGAAATA 
AAAGGGAAAG GAATGGAACT TAGTTCAGAA GTTCTTGATA TTCAGAGAGC AAAGCGAGCA 
TCCGAAATGG CCAGCGAGAA AGAATACAAA AAGGACCTGG AGTTGGAAAT TAAAGGGAAG 
GGGATGCAAA TTGACGCTGA TACCCTTGAA ATACAGCGAG TCAAAAGAGC TGCCAAGATA 
GCCAGTGAGA AAGACTATAA AAGAGATCTG GAGACAGAAA TTAAAGGGAA AGGAATGCAG 
GTCAGCACAG ACACGCTGGA TGTTCAGAGA GCTAAGAGGG CATCTGAGAT GGCCAGCCAG 
AAGCAATATA GGAAAGACTT GGAGAATGAA ATTAAGGGGA AAGGAATGCA AGTAAACGTG 
GATATCCCGG ACATGCTTCG AGCCAAGAGG GCGTCTGAAA TCTACAGCCA GAAAAAGTAT 
AAAGATGAAG CTGAGAAGAT GCTCTCCAAC TATTCCACTG TGGCAGTCAC TCCCGAGATT 
CAGAGAATCA AGACGACACA GCAAAACATT AGCAATGTAT CTTATAAGGA GGAAGTAAGA 
GCTGGCACAG CCGTAAGGAA TACTCCAGAG ATTGAACGAG TGAAGAAAAA CCAGCACAAT 
ATTAGTTCGG TGAAATTTGA AGAAGGAATT AAGCATGCAA CAGCCATTTC TGATCCTCTT 
GAGCTAAAGA GAGTTACAGA AAACCAGAAG GACGTCATCA ATAGGTTCCA GTATAAAGAG 
CCAACCTATA AGGCCACTCC GGTAACCATG ACCCCAGAGA TAGAGAGAGT GCGGAGGAAC 
CAGGAACAGC TGAGTGCGGT CAAACACAAA GGAGAGCTAA AACAAGCAAC TTCCATTTTG 
GATCTGCCAG GGCTGAAGAG AGTGAGAGAA AACCAGAAAA CTATCAGCAA TGTTTATTAT 
AAAGGCCAAT TGGGAAGGGC TACAGCTTTG AGCGTAACGC CCGAAATGGA AAGAGTAAAG 
AAGAATCAAG AAAATATCAG CTCGGTGAAA TACACTCAGG ACCATAAACA AATGAAAGGT 
AGACCATGTG TGATCTTAGA TACACCTGCT TTGAGACATG TAAAAGAAGC ACAAAATCAT 
GTTTCCATGG TCAAATATCA TGAAGATTTT GAGAAGACGA AGGGAAGAGG CTTCACTCCT 
GTTGTGGACG ATCCTGTGAC GGAGCGGGTG AGGAAGAACA CCCAGGTTGT GAGCGATGCT 
GCCTATAAAG GGGTACAGCC TCATGTTGTG GAGATGGATA GAAGACCTGG GATCATTGTT 
GACCTTAAAG TGTGGCGTAC AGATCCCGGA TCCATCTTCG ACATTGACCC CCTGGAGGAC 
AATATTCAGT CTCGAAGTCT CCATATGCTC TCTGAAAAGG CAAGTCAGTA CCAGAGCCGC 
CTGTCTCGCT CACACTCTGG CAGCACTTTT GGTACGGGTC TGGGAGATGA CAAGTCAGAG 
ATCTCCGAGC TCTACCCTAG CTTTTCCTGT TGCAGTGAGG TAACAAGACC ATCTGATGAA 
GGAGCACCCG TCCTTCCTGG AGCTTACCAG CAGAGCCACT CCCAAGGCTA CGGGTACATG 
CACCAGACCA GCGTGTCGTC CGTGAGGTCA ATGCAGCATT CAGCAAATCT AAGGACCTAC 
CGAGCCATGT ACGATTACAG TGCCCAGGAC GAAGATGAGG TCTCCTTCCG AGATGGTGAC 
TACATCGTCA ACGTGCAGCC CATTGATGAC GGCTGGATGT ATGGCACCGT GCAGAGAACC 
GGGAGAACGG GCATGCTCCC AGCGAATTAC ATTGAGTTTG TGAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178623.1
CDS	80..3127
Translation	MKVPVSGDVKEETEEENVEEEEKPEDEVFLKPVVEDLSMELARKCTELISDIHYKEDYRKSKDKCTSVTDTPTLNHVKNISAFISETKYKGTIKADLSNCLYKDMPATIDSVFAREVSQLQSEVAYKQKHEAEKGLSDYAHMKEPPEVRHAMEVNRHQSNISYRKDVQGTHTYTAEMDRPDIKKATQISKIISNAEYKKGQGIVNKEPSVIGRPDFEHAVEASKLSSQVKYKEKFDNEMKGKGHHYNPLGSASFRQHQLATVLASDVKYKKDVQTMHEPVSDLPNLLFLDHALKASRMLSGWEYKKNFEENKGSYHFDAEAPEHLHHRGNATLQSQVKYREEYEKNKGKTMLDFVETPSYQSSKEAQKMQSEKVYKEDFEKEIKGRSSLDLDKTPAFLHVKHISNLMREKEYKKDLENEIKGKGMELSSEVLDIQRAKRASEMASEKEYKKDLELEIKGKGMQIDADTLEIQRVKRAAKIASEKDYKRDLETEIKGKGMQVSTDTLDVQRAKRASEMASQKQYRKDLENEIKGKGMQVNVDIPDMLRAKRASEIYSQKKYKDEAEKMLSNYSTVAVTPEIQRIKTTQQNISNVSYKEEVRAGTAVRNTPEIERVKKNQHNISSVKFEEGIKHATAISDPLELKRVTENQKDVINRFQYKEPTYKATPVTMTPEIERVRRNQEQLSAVKHKGELKQATSILDLPGLKRVRENQKTISNVYYKGQLGRATALSVTPEMERVKKNQENISSVKYTQDHKQMKGRPCVILDTPALRHVKEAQNHVSMVKYHEDFEKTKGRGFTPVVDDPVTERVRKNTQVVSDAAYKGVQPHVVEMDRRPGIIVDLKVWRTDPGSIFDIDPLEDNIQSRSLHMLSEKASQYQSRLSRSHSGSTFGTGLGDDKSEISELYPSFSCCSEVTRPSDEGAPVLPGAYQQSHSQGYGYMHQTSVSSVRSMQHSANLRTYRAMYDYSAQDEDEVSFRDGDYIVNVQPIDDGWMYGTVQRTGRTGMLPANYIEFVN

Target ORF information:

RefSeq Version	NM_001191694.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nebulette (Nebl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191694.1

ORF Insert Sequence:

ATGAAGGTGC CTGTATCCGG GGATGTGAAA GAGGAAACCG AAGAGGAAAA TGTGGAGGAG 
GAAGAAAAGC CAGAAGACGA GGTGTTTCTG AAACCTGTCG TTGAAGACCT AAGCATGGAG 
CTGGCCAGAA AGTGCACCGA GCTCATTAGT GACATCCATT ATAAAGAAGA CTATAGAAAG 
TCTAAGGACA AGTGTACGTC TGTGACTGAT ACCCCTACGC TAAACCATGT GAAAAATATT 
AGTGCTTTTA TTTCTGAGAC AAAATACAAA GGTACCATTA AAGCTGATCT TTCTAACTGT 
CTGTATAAGG ACATGCCTGC TACCATTGAC AGTGTCTTTG CAAGAGAAGT GTCCCAGCTT 
CAGAGTGAGG TTGCCTATAA GCAGAAGCAT GAGGCGGAGA AAGGCCTCTC CGATTATGCC 
CACATGAAGG AACCACCGGA GGTCAGACAC GCCATGGAGG TCAACAGGCA CCAGAGTAAC 
ATTTCTTACA GAAAAGATGT GCAAGGCACC CACACGTATA CAGCGGAGAT GGACAGACCG 
GATATCAAGA AGGCAACTCA GATCTCCAAG ATCATAAGCA ATGCAGAATA CAAGAAAGGA 
CAAGGCATCG TGAATAAAGA ACCTTCTGTA ATTGGGAGAC CAGATTTTGA ACATGCCGTG 
GAAGCTTCTA AACTTTCTAG TCAGGTTAAA TATAAAGAAA AGTTTGATAA TGAAATGAAG 
GGAAAAGGCC ACCATTACAA CCCTCTGGGA AGTGCGTCTT TCAGACAACA CCAGCTCGCT 
ACTGTCCTGG CAAGCGATGT GAAGTACAAG AAAGATGTTC AAACCATGCA CGAGCCAGTT 
TCAGATCTCC CAAACTTGTT GTTCTTAGAC CATGCTCTGA AAGCCAGCAG AATGCTCAGT 
GGATGGGAAT ATAAAAAGAA TTTTGAAGAA AACAAAGGAT CGTATCACTT TGATGCAGAG 
GCCCCTGAAC ACCTACACCA TAGAGGAAAT GCCACCCTCC AGAGTCAGGT AAAATACAGA 
GAAGAATATG AGAAGAATAA GGGGAAGACC ATGCTTGACT TTGTTGAGAC ACCATCATAT 
CAGTCTTCAA AGGAGGCTCA GAAGATGCAG AGCGAGAAAG TTTACAAAGA AGATTTTGAG 
AAGGAGATTA AAGGAAGATC CTCACTGGAT TTAGACAAGA CTCCTGCATT TCTACACGTG 
AAGCACATCA GCAACCTGAT GAGGGAGAAA GAATATAAAA AAGATTTGGA AAATGAAATA 
AAAGGGAAAG GAATGGAACT TAGTTCAGAA GTTCTTGATA TTCAGAGAGC AAAGCGAGCA 
TCCGAAATGG CCAGCGAGAA AGAATACAAA AAGGACCTGG AGTTGGAAAT TAAAGGGAAG 
GGGATGCAAA TTGACGCTGA TACCCTTGAA ATACAGCGAG TCAAAAGAGC TGCCAAGATA 
GCCAGTGAGA AAGACTATAA AAGAGATCTG GAGACAGAAA TTAAAGGGAA AGGAATGCAG 
GTCAGCACAG ACACGCTGGA TGTTCAGAGA GCTAAGAGGG CATCTGAGAT GGCCAGCCAG 
AAGCAATATA GGAAAGACTT GGAGAATGAA ATTAAGGGGA AAGGAATGCA AGTAAACGTG 
GATATCCCGG ACATGCTTCG AGCCAAGAGG GCGTCTGAAA TCTACAGCCA GAAAAAGTAT 
AAAGATGAAG CTGAGAAGAT GCTCTCCAAC TATTCCACTG TGGCAGTCAC TCCCGAGATT 
CAGAGAATCA AGACGACACA GCAAAACATT AGCAATGTAT CTTATAAGGA GGAAGTAAGA 
GCTGGCACAG CCGTAAGGAA TACTCCAGAG ATTGAACGAG TGAAGAAAAA CCAGCACAAT 
ATTAGTTCGG TGAAATTTGA AGAAGGAATT AAGCATGCAA CAGCCATTTC TGATCCTCTT 
GAGCTAAAGA GAGTTACAGA AAACCAGAAG GACGTCATCA ATAGGTTCCA GTATAAAGAG 
CCAACCTATA AGGCCACTCC GGTAACCATG ACCCCAGAGA TAGAGAGAGT GCGGAGGAAC 
CAGGAACAGC TGAGTGCGGT CAAACACAAA GGAGAGCTAA AACAAGCAAC TTCCATTTTG 
GATCTGCCAG GGCTGAAGAG AGTGAGAGAA AACCAGAAAA CTATCAGCAA TGTTTATTAT 
AAAGGCCAAT TGGGAAGGGC TACAGCTTTG AGCGTAACGC CCGAAATGGA AAGAGTAAAG 
AAGAATCAAG AAAATATCAG CTCGGTGAAA TACACTCAGG ACCATAAACA AATGAAAGGT 
AGACCATGTG TGATCTTAGA TACACCTGCT TTGAGACATG TAAAAGAAGC ACAAAATCAT 
GTTTCCATGG TCAAATATCA TGAAGATTTT GAGAAGACGA AGGGAAGAGG CTTCACTCCT 
GTTGTGGACG ATCCTGTGAC GGAGCGGGTG AGGAAGAACA CCCAGGTTGT GAGCGATGCT 
GCCTATAAAG GGGTACAGCC TCATGTTGTG GAGATGGATA GAAGACCTGG GATCATTGTT 
GACCTTAAAG TGTGGCGTAC AGATCCCGGA TCCATCTTCG ACATTGACCC CCTGGAGGAC 
AATATTCAGT CTCGAAGTCT CCATATGCTC TCTGAAAAGG CAAGTCAGTA CCAGAGCCGC 
CTGTCTCGCT CACACTCTGG CAGCACTTTT GGTACGGGTC TGGGAGATGA CAAGTCAGAG 
ATCTCCGAGC TCTACCCTAG CTTTTCCTGT TGCAGTGAGG TAACAAGACC ATCTGATGAA 
GGAGCACCCG TCCTTCCTGG AGCTTACCAG CAGAGCCACT CCCAAGGCTA CGGGTACATG 
CACCAGACCA GCGTGTCGTC CGTGAGGTCA ATGCAGCATT CAGCAAATCT AAGGACCTAC 
CGAGCCATGT ACGATTACAG TGCCCAGGAC GAAGATGAGG TCTCCTTCCG AGATGGTGAC 
TACATCGTCA ACGTGCAGCC CATTGATGAC GGCTGGATGT ATGGCACCGT GCAGAGAACC 
GGGAGAACGG GCATGCTCCC AGCGAATTAC ATTGAGTTTG TGAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nebulette mutations are associated with dilated cardiomyopathy and endocardial fibroelastosis.
Journal of the American College of Cardiology56(18)1493-502(2010 Oct)
Purevjav E,Varela J,Morgado M,Kearney DL,Li H,Taylor MD,Arimura T,Moncman CL,McKenna W,Murphy RT,Labeit S,Vatta M,Bowles NE,Kimura A,Boriek AM,Towbin JA

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)
Gonzales PA,Pisitkun T,Hoffert JD,Tchapyjnikov D,Star RA,Kleta R,Wang NS,Knepper MA

The nebulette repeat domain is necessary for proper maintenance of tropomyosin with the cardiac sarcomere.
Experimental cell research314(19)3519-30(2008 Nov)
Bonzo JR,Norris AA,Esham M,Moncman CL

Nebulette interacts with filamin C.
Cell motility and the cytoskeleton65(2)130-42(2008 Feb)
Holmes WB,Moncman CL

Targeted disruption of nebulette protein expression alters cardiac myofibril assembly and function.
Experimental cell research273(2)204-18(2002 Feb)
Moncman CL,Wang K