Sema5a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Gene Symbol	Sema5a
Entrez Gene ID	310207
Full Name	semaphorin 5A
General protein information	Preferred Names semaphorin 5A Names semaphorin-5A sema F sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A semaphorin-F
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q22

mRNA and Protein(s)

mRNA Protein Name

XM_017590833.1 XP_017446322.1 semaphorin-5A isoform X1

NM_001107659.2 NP_001101129.1 semaphorin-5A precursor
Pathways from BioSystems

mRNA	Protein	Name
XM_017590833.1	XP_017446322.1	semaphorin-5A isoform X1
NM_001107659.2	NP_001101129.1	semaphorin-5A precursor

Homologies

Canis lupus familiaris (dog)	SEMA5A	XP_005639731.1
Rattus norvegicus (Norway rat)	Sema5a	NP_001101129.1
Bos taurus (cattle)	SEMA5A	XP_005221710.1
Gallus gallus (chicken)	SEMA5A	XP_419016.3
Danio rerio (zebrafish)	sema5a	NP_001258599.1
Pan troglodytes (chimpanzee)	SEMA5A	XP_517627.2
Mus musculus (house mouse)	Sema5a	NP_033180.2
Homo sapiens (human)	SEMA5A	NP_003957.2
	SEMA5A	XP_002804352.1

Gene Ontology
Bibliography

Related articles in PubMed

Class 5 transmembrane semaphorins control selective Mammalian retinal lamination and function.
Matsuoka RL, Chivatakarn O, Badea TC, Samuels IS, Cahill H, Katayama K, Kumar SR, Suto F, Ch?dotal A, Peachey NS, Nathans J, Yoshida Y, Giger RJ, Kolodkin AL
Neuron71(3)460-73(2011 Aug)

Semaphorin 5A promotes angiogenesis by increasing endothelial cell proliferation, migration, and decreasing apoptosis.
Sadanandam A, Rosenbaugh EG, Singh S, Varney M, Singh RK
Microvascular research79(1)1-9(2010 Jan)

Semaphorin5A expression in the developing chick telencephalon.
Pineda D, Garc?a B, Olmos JL, D?vila JC, Real MA, Guirado S
Brain research bulletin66(4-6)436-40(2005 Sep)

Inactivation of the Sema5a gene results in embryonic lethality and defective remodeling of the cranial vascular system.
Fiore R, Rahim B, Christoffels VM, Moorman AF, P?schel AW
Molecular and cellular biology25(6)2310-9(2005 Mar)

Semaphorin 5A is a bifunctional axon guidance cue regulated by heparan and chondroitin sulfate proteoglycans.
Kantor DB, Chivatakarn O, Peer KL, Oster SF, Inatani M, Hansen MJ, Flanagan JG, Yamaguchi Y, Sretavan DW, Giger RJ, Kolodkin AL
Neuron44(6)961-75(2004 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Semaphorin5A expression in the developing chick telencephalon.
Title: Semaphorin5A expression in the developing chick telencephalon.

The following Sema5a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sema5a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05493	NM_001107659.2 Latest version!	Rattus norvegicus semaphorin 5A (Sema5a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa05493	XM_017590833.1 Latest version!	Rattus norvegicus semaphorin 5A (Sema5a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sema5a ( NM_001107659.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101129.1 Rattus norvegicus semaphorin 5A (Sema5a), mRNA.

Price & Availability↑

CloneID	ORa05493
Clone ID Related Accession (Same CDS sequence)	XM_017590833.1 , NM_001107659.2
Accession Version	NM_001107659.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	semaphorin-5A precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07009051.1 and AABR07009052.1. On Jan 9, 2008 this sequence version replaced NM_001107659.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGGGAG CCTGCATCCT TGCATGGCTG TTCTCAAGCC TGGGAGTGTG GAGACTTGCT 
CGGCCTGAGA CCCAGGACCC TGCCAAGTGC CAGAGAGCTG AGCACCCCGT CGTCTCCTAC 
AAAGAAATTG GCCCCTGGTT ACGGGAATTC AGAGCCGAGA ATGCTGTGGA TTTCTCGAGG 
TTAACATTTG ACCCAGGACA GAAAGAACTT GTCGTAGGAG CAAGAAACTA TCTCTTCAGA 
CTACAGCTCG AGGATCTGTC TCTCATCCAG GCTGTGCAGT GGGAGTGTGA TGAAGCCACG 
AAGAAGGCCT GTTACAGCAA GGGCAAATCG AAGGAGGAAT GTCAGAACTA CATACGAGTG 
CTCTTAGTGG GCGGGGACCG GCTCTTCACC TGTGGGACCA ATGCCTTCAC ACCCGTCTGT 
ACCATCCGCT CCTTAAGTAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCTCGA 
TGTCCCTATA GTCCCCAGCA CAATTCTACA GCTCTACTCA CTGCCAGTGG CGAGCTCTAT 
GCTGCAACAG CCATGGATTT CCCAGGGCGC GATCCAGCCA TTTATCGAAG TCTGGGCACT 
TTACCTCCTC TTCGAACTGC ACAGTACAAC TCCAAATGGC TCAATGAACC AAACTTTGTG 
TCTTCCTACG ACATCGGGAA CTTCACCTAC TTCTTCTTCC GAGAAAATGC CGTGGAACAC 
GACTGTGGGA AGACTGTGTT CTCCAGGGCA GCCAGGGTCT GCAAGAATGA CATTGGAGGA 
CGATTTCTTC TGGAGGATAC CTGGACTACC TTCATGAAGG CTCGCCTCAA CTGCTCTCGG 
CCTGGTGAGG TGCCGTTCTA CTACAACGAG CTTCAAAGCA CCTTCTTCCT GCCGGAGCTG 
GATCTGATCT ACGGCATCTT CACCACCAAT GTAAACAGCA TTGCTGCCTC TGCTGTCTGT 
GTCTTCAACC TGAGCGCCAT CTCACAGGCC TTCAATGGAC CCTTCAAGTA CCAAGAGAAC 
TCTCGCTCAG CCTGGCTGCC TTATCCCAAC CCCAACCCCA ATTTTCAGTG CGGCACCATG 
GACCAGGGCC TGTATGTAAA CCTGACGGAA AGAAACCTAC AGGATGCTCA GAAGTTCATC 
CTGATGCATG AAGTGGTGCA GCCAGTGACC ACAGTGCCTT CCTTCATGGA AGACAACAGC 
CGTTTCTCCC ATGTGGCTGT CGATGTTGTG CAAGGCAGGG ACACCCTTGT CCATATCATC 
TATCTGGCCA CAGATTATGG CACCATTAAA AAAGTGCGGG CTCCGCTGAG TCAGAGCTCG 
GGCAGCTGTT TGCTAGAAGA GATTGAGCTT TTTCCAGAGA GGAAGAGTGA GCCCATCAGG 
AGCCTAAAGA TCCTCCACAG CCAGAGTGTC CTATTTGTGG GGCTACAGGA GCATGTGGTC 
AAGATCCCCC TGAAGAGATG CCATTTTCAT CAAACACGTG GTGCCTGCAT TGGTGCTCAG 
GACCCTTACT GTGGCTGGGA TGCGGTGATG AAGAAGTGCA CCAGCCTGGA GGAGAGCCTG 
AGCATGACCC AGTGGGATCA GAGCGTCCCC ACCTGTCCGA CCAGAAATCT CACCGTAGAT 
GGGAGCTTTG GCCCGTGGTC TCCATGGACA CCCTGTACTC ACACTGATGG CACTGCTGTG 
GGATCCTGCC TCTGCCGGTC CCGATCCTGT GACAGCCCAG CTCCACAGTG TGGCGGCTGG 
CAGTGTGAGG GCCCTAGAAT GGAGATCACC AACTGTTCCA GGAATGGAGG CTGGACTCCC 
TGGACCTCCT GGTCTCCCTG CAGCACCACT TGTGGCATTG GCTTCCAGGT GCGGCAGCGC 
TCCTGCAGCA ACCCTACGCC CAGGCATGGA GGGCGTGTGT GCGTGGGCCA GAACAGGGAG 
GAAAGATACT GCAATGAACA TTTACTCTGC CCACCACACG TGTTCTGGAC AGGCTGGGGA 
CCTTGGGAAC GCTGCACAGC CCAGTGCGGG GGTGGCATTC AAGCTCGCCG TAGGACCTGT 
GAAAATGGGC CTGACTGTGC AGGATGCAAT GTGGAATACC AGCCTTGTAA CACCAATGCA 
TGCCCAGAGT TGAAGAAGAC CACACCATGG ACCCCCTGGA CACCTGTCAA CATCTCTGAC 
AATGGGGGCC ACTATGAGCA GCGGTTCCGC TACACCTGTA AAGCTCGCCT GCCAGATCCA 
AACTTATTGG AAGTAGGAAG ACAAAGGATA GAAATGCGGT ACTGTTCTAG TGATGGGACC 
AGTGGCTGCT CCACAGATGG ACTTTCTGGA GACTTTCTAA GAGCTGGGAG ATACTCTGCC 
CATACAGTCA ATGGGGCATG GTCAGCCTGG ACTTCCTGGT CACAGTGCAG CAGAGACTGC 
AGCAGGGGCA TTCGAAACAG GAAGCGTGTT TGCAACAACC CAGAGCCCAA GTATGGAGGT 
ATGCCATGCC TTGGTCCATC ACTGGAGTTC CAGGAATGCA ACATTTTACC CTGTCCAGTG 
GATGGTGTGT GGTCTTGCTG GTCATCCTGG TCTAAATGCT CAGCAACTTG TGGGGGTGGA 
CACTACATGA GAACCCGTTC GTGTACGAAT CCAGCCCCAG CATACGGAGG GGACATCTGC 
CTGGGACTGC ACACGGAAGA GGCCCTCTGT AACACACAGA CCTGCCCAGA GAACTGGTCA 
GAGTGGTCAG AGTGGTCTGT GTGTGATGCA TCTGGTACCC AGGTCCGTAC TCGGCAGTGC 
ATCCTTCTGT TTCCTGTGGG CAGCCAGTGT TCTGGAAATA CTACAGAGAG CCGGCCTTGT 
GTATTTGACT CTAATTTCAT CCCAGAAGTG TCTGTGGCAA GATCCAGCAG CGTAGAAGAG 
AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATGGCTG TGGGACTTAG CAGTTCCATC 
CTCGGCTGCC TCCTTACACT GCTTGTCTAC ACCTACTGCC AGCGGTACCA GCAGCAGTCC 
CATGATGCAA CTGTCATCCA TCCTGTTTCT CCCGCCGCCC TCAACAGCAG CATAACTAAC 
CACATCAACA AACTGGATAA ATACGATTCC GTGGAGGCCA TCAAGGCATT TAACAAAAAC 
AACTTGATCC TAGAGGAGAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAC 
TCCAACGCCT ACTTTACAGA TCTCAACAAT TATGATGAAT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101129.1
CDS	667..3891
Translation	MKGACILAWLFSSLGVWRLARPETQDPAKCQRAEHPVVSYKEIGPWLREFRAENAVDFSRLTFDPGQKELVVGARNYLFRLQLEDLSLIQAVQWECDEATKKACYSKGKSKEECQNYIRVLLVGGDRLFTCGTNAFTPVCTIRSLSNLTEIHDQISGMARCPYSPQHNSTALLTASGELYAATAMDFPGRDPAIYRSLGTLPPLRTAQYNSKWLNEPNFVSSYDIGNFTYFFFRENAVEHDCGKTVFSRAARVCKNDIGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQSTFFLPELDLIYGIFTTNVNSIAASAVCVFNLSAISQAFNGPFKYQENSRSAWLPYPNPNPNFQCGTMDQGLYVNLTERNLQDAQKFILMHEVVQPVTTVPSFMEDNSRFSHVAVDVVQGRDTLVHIIYLATDYGTIKKVRAPLSQSSGSCLLEEIELFPERKSEPIRSLKILHSQSVLFVGLQEHVVKIPLKRCHFHQTRGACIGAQDPYCGWDAVMKKCTSLEESLSMTQWDQSVPTCPTRNLTVDGSFGPWSPWTPCTHTDGTAVGSCLCRSRSCDSPAPQCGGWQCEGPRMEITNCSRNGGWTPWTSWSPCSTTCGIGFQVRQRSCSNPTPRHGGRVCVGQNREERYCNEHLLCPPHVFWTGWGPWERCTAQCGGGIQARRRTCENGPDCAGCNVEYQPCNTNACPELKKTTPWTPWTPVNISDNGGHYEQRFRYTCKARLPDPNLLEVGRQRIEMRYCSSDGTSGCSTDGLSGDFLRAGRYSAHTVNGAWSAWTSWSQCSRDCSRGIRNRKRVCNNPEPKYGGMPCLGPSLEFQECNILPCPVDGVWSCWSSWSKCSATCGGGHYMRTRSCTNPAPAYGGDICLGLHTEEALCNTQTCPENWSEWSEWSVCDASGTQVRTRQCILLFPVGSQCSGNTTESRPCVFDSNFIPEVSVARSSSVEEKRCGEFNMFHMMAVGLSSSILGCLLTLLVYTYCQRYQQQSHDATVIHPVSPAALNSSITNHINKLDKYDSVEAIKAFNKNNLILEERNKYFNPHLTGKTYSNAYFTDLNNYDEY

Target ORF information:

RefSeq Version	NM_001107659.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus semaphorin 5A (Sema5a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107659.2

ORF Insert Sequence:

ATGAAGGGAG CCTGCATCCT TGCATGGCTG TTCTCAAGCC TGGGAGTGTG GAGACTTGCT 
CGGCCTGAGA CCCAGGACCC TGCCAAGTGC CAGAGAGCTG AGCACCCCGT CGTCTCCTAC 
AAAGAAATTG GCCCCTGGTT ACGGGAATTC AGAGCCGAGA ATGCTGTGGA TTTCTCGAGG 
TTAACATTTG ACCCAGGACA GAAAGAACTT GTCGTAGGAG CAAGAAACTA TCTCTTCAGA 
CTACAGCTCG AGGATCTGTC TCTCATCCAG GCTGTGCAGT GGGAGTGTGA TGAAGCCACG 
AAGAAGGCCT GTTACAGCAA GGGCAAATCG AAGGAGGAAT GTCAGAACTA CATACGAGTG 
CTCTTAGTGG GCGGGGACCG GCTCTTCACC TGTGGGACCA ATGCCTTCAC ACCCGTCTGT 
ACCATCCGCT CCTTAAGTAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCTCGA 
TGTCCCTATA GTCCCCAGCA CAATTCTACA GCTCTACTCA CTGCCAGTGG CGAGCTCTAT 
GCTGCAACAG CCATGGATTT CCCAGGGCGC GATCCAGCCA TTTATCGAAG TCTGGGCACT 
TTACCTCCTC TTCGAACTGC ACAGTACAAC TCCAAATGGC TCAATGAACC AAACTTTGTG 
TCTTCCTACG ACATCGGGAA CTTCACCTAC TTCTTCTTCC GAGAAAATGC CGTGGAACAC 
GACTGTGGGA AGACTGTGTT CTCCAGGGCA GCCAGGGTCT GCAAGAATGA CATTGGAGGA 
CGATTTCTTC TGGAGGATAC CTGGACTACC TTCATGAAGG CTCGCCTCAA CTGCTCTCGG 
CCTGGTGAGG TGCCGTTCTA CTACAACGAG CTTCAAAGCA CCTTCTTCCT GCCGGAGCTG 
GATCTGATCT ACGGCATCTT CACCACCAAT GTAAACAGCA TTGCTGCCTC TGCTGTCTGT 
GTCTTCAACC TGAGCGCCAT CTCACAGGCC TTCAATGGAC CCTTCAAGTA CCAAGAGAAC 
TCTCGCTCAG CCTGGCTGCC TTATCCCAAC CCCAACCCCA ATTTTCAGTG CGGCACCATG 
GACCAGGGCC TGTATGTAAA CCTGACGGAA AGAAACCTAC AGGATGCTCA GAAGTTCATC 
CTGATGCATG AAGTGGTGCA GCCAGTGACC ACAGTGCCTT CCTTCATGGA AGACAACAGC 
CGTTTCTCCC ATGTGGCTGT CGATGTTGTG CAAGGCAGGG ACACCCTTGT CCATATCATC 
TATCTGGCCA CAGATTATGG CACCATTAAA AAAGTGCGGG CTCCGCTGAG TCAGAGCTCG 
GGCAGCTGTT TGCTAGAAGA GATTGAGCTT TTTCCAGAGA GGAAGAGTGA GCCCATCAGG 
AGCCTAAAGA TCCTCCACAG CCAGAGTGTC CTATTTGTGG GGCTACAGGA GCATGTGGTC 
AAGATCCCCC TGAAGAGATG CCATTTTCAT CAAACACGTG GTGCCTGCAT TGGTGCTCAG 
GACCCTTACT GTGGCTGGGA TGCGGTGATG AAGAAGTGCA CCAGCCTGGA GGAGAGCCTG 
AGCATGACCC AGTGGGATCA GAGCGTCCCC ACCTGTCCGA CCAGAAATCT CACCGTAGAT 
GGGAGCTTTG GCCCGTGGTC TCCATGGACA CCCTGTACTC ACACTGATGG CACTGCTGTG 
GGATCCTGCC TCTGCCGGTC CCGATCCTGT GACAGCCCAG CTCCACAGTG TGGCGGCTGG 
CAGTGTGAGG GCCCTAGAAT GGAGATCACC AACTGTTCCA GGAATGGAGG CTGGACTCCC 
TGGACCTCCT GGTCTCCCTG CAGCACCACT TGTGGCATTG GCTTCCAGGT GCGGCAGCGC 
TCCTGCAGCA ACCCTACGCC CAGGCATGGA GGGCGTGTGT GCGTGGGCCA GAACAGGGAG 
GAAAGATACT GCAATGAACA TTTACTCTGC CCACCACACG TGTTCTGGAC AGGCTGGGGA 
CCTTGGGAAC GCTGCACAGC CCAGTGCGGG GGTGGCATTC AAGCTCGCCG TAGGACCTGT 
GAAAATGGGC CTGACTGTGC AGGATGCAAT GTGGAATACC AGCCTTGTAA CACCAATGCA 
TGCCCAGAGT TGAAGAAGAC CACACCATGG ACCCCCTGGA CACCTGTCAA CATCTCTGAC 
AATGGGGGCC ACTATGAGCA GCGGTTCCGC TACACCTGTA AAGCTCGCCT GCCAGATCCA 
AACTTATTGG AAGTAGGAAG ACAAAGGATA GAAATGCGGT ACTGTTCTAG TGATGGGACC 
AGTGGCTGCT CCACAGATGG ACTTTCTGGA GACTTTCTAA GAGCTGGGAG ATACTCTGCC 
CATACAGTCA ATGGGGCATG GTCAGCCTGG ACTTCCTGGT CACAGTGCAG CAGAGACTGC 
AGCAGGGGCA TTCGAAACAG GAAGCGTGTT TGCAACAACC CAGAGCCCAA GTATGGAGGT 
ATGCCATGCC TTGGTCCATC ACTGGAGTTC CAGGAATGCA ACATTTTACC CTGTCCAGTG 
GATGGTGTGT GGTCTTGCTG GTCATCCTGG TCTAAATGCT CAGCAACTTG TGGGGGTGGA 
CACTACATGA GAACCCGTTC GTGTACGAAT CCAGCCCCAG CATACGGAGG GGACATCTGC 
CTGGGACTGC ACACGGAAGA GGCCCTCTGT AACACACAGA CCTGCCCAGA GAACTGGTCA 
GAGTGGTCAG AGTGGTCTGT GTGTGATGCA TCTGGTACCC AGGTCCGTAC TCGGCAGTGC 
ATCCTTCTGT TTCCTGTGGG CAGCCAGTGT TCTGGAAATA CTACAGAGAG CCGGCCTTGT 
GTATTTGACT CTAATTTCAT CCCAGAAGTG TCTGTGGCAA GATCCAGCAG CGTAGAAGAG 
AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATGGCTG TGGGACTTAG CAGTTCCATC 
CTCGGCTGCC TCCTTACACT GCTTGTCTAC ACCTACTGCC AGCGGTACCA GCAGCAGTCC 
CATGATGCAA CTGTCATCCA TCCTGTTTCT CCCGCCGCCC TCAACAGCAG CATAACTAAC 
CACATCAACA AACTGGATAA ATACGATTCC GTGGAGGCCA TCAAGGCATT TAACAAAAAC 
AACTTGATCC TAGAGGAGAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAC 
TCCAACGCCT ACTTTACAGA TCTCAACAAT TATGATGAAT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema5a ( XM_017590833.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446322.1 Rattus norvegicus semaphorin 5A (Sema5a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa05493
Clone ID Related Accession (Same CDS sequence)	XM_017590833.1 , NM_001107659.2
Accession Version	XM_017590833.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	semaphorin-5A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGAG CCTGCATCCT TGCATGGCTG TTCTCAAGCC TGGGAGTGTG GAGACTTGCT 
CGGCCTGAGA CCCAGGACCC TGCCAAGTGC CAGAGAGCTG AGCACCCCGT CGTCTCCTAC 
AAAGAAATTG GCCCCTGGTT ACGGGAATTC AGAGCCGAGA ATGCTGTGGA TTTCTCGAGG 
TTAACATTTG ACCCAGGACA GAAAGAACTT GTCGTAGGAG CAAGAAACTA TCTCTTCAGA 
CTACAGCTCG AGGATCTGTC TCTCATCCAG GCTGTGCAGT GGGAGTGTGA TGAAGCCACG 
AAGAAGGCCT GTTACAGCAA GGGCAAATCG AAGGAGGAAT GTCAGAACTA CATACGAGTG 
CTCTTAGTGG GCGGGGACCG GCTCTTCACC TGTGGGACCA ATGCCTTCAC ACCCGTCTGT 
ACCATCCGCT CCTTAAGTAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCTCGA 
TGTCCCTATA GTCCCCAGCA CAATTCTACA GCTCTACTCA CTGCCAGTGG CGAGCTCTAT 
GCTGCAACAG CCATGGATTT CCCAGGGCGC GATCCAGCCA TTTATCGAAG TCTGGGCACT 
TTACCTCCTC TTCGAACTGC ACAGTACAAC TCCAAATGGC TCAATGAACC AAACTTTGTG 
TCTTCCTACG ACATCGGGAA CTTCACCTAC TTCTTCTTCC GAGAAAATGC CGTGGAACAC 
GACTGTGGGA AGACTGTGTT CTCCAGGGCA GCCAGGGTCT GCAAGAATGA CATTGGAGGA 
CGATTTCTTC TGGAGGATAC CTGGACTACC TTCATGAAGG CTCGCCTCAA CTGCTCTCGG 
CCTGGTGAGG TGCCGTTCTA CTACAACGAG CTTCAAAGCA CCTTCTTCCT GCCGGAGCTG 
GATCTGATCT ACGGCATCTT CACCACCAAT GTAAACAGCA TTGCTGCCTC TGCTGTCTGT 
GTCTTCAACC TGAGCGCCAT CTCACAGGCC TTCAATGGAC CCTTCAAGTA CCAAGAGAAC 
TCTCGCTCAG CCTGGCTGCC TTATCCCAAC CCCAACCCCA ATTTTCAGTG CGGCACCATG 
GACCAGGGCC TGTATGTAAA CCTGACGGAA AGAAACCTAC AGGATGCTCA GAAGTTCATC 
CTGATGCATG AAGTGGTGCA GCCAGTGACC ACAGTGCCTT CCTTCATGGA AGACAACAGC 
CGTTTCTCCC ATGTGGCTGT CGATGTTGTG CAAGGCAGGG ACACCCTTGT CCATATCATC 
TATCTGGCCA CAGATTATGG CACCATTAAA AAAGTGCGGG CTCCGCTGAG TCAGAGCTCG 
GGCAGCTGTT TGCTAGAAGA GATTGAGCTT TTTCCAGAGA GGAAGAGTGA GCCCATCAGG 
AGCCTAAAGA TCCTCCACAG CCAGAGTGTC CTATTTGTGG GGCTACAGGA GCATGTGGTC 
AAGATCCCCC TGAAGAGATG CCATTTTCAT CAAACACGTG GTGCCTGCAT TGGTGCTCAG 
GACCCTTACT GTGGCTGGGA TGCGGTGATG AAGAAGTGCA CCAGCCTGGA GGAGAGCCTG 
AGCATGACCC AGTGGGATCA GAGCGTCCCC ACCTGTCCGA CCAGAAATCT CACCGTAGAT 
GGGAGCTTTG GCCCGTGGTC TCCATGGACA CCCTGTACTC ACACTGATGG CACTGCTGTG 
GGATCCTGCC TCTGCCGGTC CCGATCCTGT GACAGCCCAG CTCCACAGTG TGGCGGCTGG 
CAGTGTGAGG GCCCTAGAAT GGAGATCACC AACTGTTCCA GGAATGGAGG CTGGACTCCC 
TGGACCTCCT GGTCTCCCTG CAGCACCACT TGTGGCATTG GCTTCCAGGT GCGGCAGCGC 
TCCTGCAGCA ACCCTACGCC CAGGCATGGA GGGCGTGTGT GCGTGGGCCA GAACAGGGAG 
GAAAGATACT GCAATGAACA TTTACTCTGC CCACCACACG TGTTCTGGAC AGGCTGGGGA 
CCTTGGGAAC GCTGCACAGC CCAGTGCGGG GGTGGCATTC AAGCTCGCCG TAGGACCTGT 
GAAAATGGGC CTGACTGTGC AGGATGCAAT GTGGAATACC AGCCTTGTAA CACCAATGCA 
TGCCCAGAGT TGAAGAAGAC CACACCATGG ACCCCCTGGA CACCTGTCAA CATCTCTGAC 
AATGGGGGCC ACTATGAGCA GCGGTTCCGC TACACCTGTA AAGCTCGCCT GCCAGATCCA 
AACTTATTGG AAGTAGGAAG ACAAAGGATA GAAATGCGGT ACTGTTCTAG TGATGGGACC 
AGTGGCTGCT CCACAGATGG ACTTTCTGGA GACTTTCTAA GAGCTGGGAG ATACTCTGCC 
CATACAGTCA ATGGGGCATG GTCAGCCTGG ACTTCCTGGT CACAGTGCAG CAGAGACTGC 
AGCAGGGGCA TTCGAAACAG GAAGCGTGTT TGCAACAACC CAGAGCCCAA GTATGGAGGT 
ATGCCATGCC TTGGTCCATC ACTGGAGTTC CAGGAATGCA ACATTTTACC CTGTCCAGTG 
GATGGTGTGT GGTCTTGCTG GTCATCCTGG TCTAAATGCT CAGCAACTTG TGGGGGTGGA 
CACTACATGA GAACCCGTTC GTGTACGAAT CCAGCCCCAG CATACGGAGG GGACATCTGC 
CTGGGACTGC ACACGGAAGA GGCCCTCTGT AACACACAGA CCTGCCCAGA GAACTGGTCA 
GAGTGGTCAG AGTGGTCTGT GTGTGATGCA TCTGGTACCC AGGTCCGTAC TCGGCAGTGC 
ATCCTTCTGT TTCCTGTGGG CAGCCAGTGT TCTGGAAATA CTACAGAGAG CCGGCCTTGT 
GTATTTGACT CTAATTTCAT CCCAGAAGTG TCTGTGGCAA GATCCAGCAG CGTAGAAGAG 
AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATGGCTG TGGGACTTAG CAGTTCCATC 
CTCGGCTGCC TCCTTACACT GCTTGTCTAC ACCTACTGCC AGCGGTACCA GCAGCAGTCC 
CATGATGCAA CTGTCATCCA TCCTGTTTCT CCCGCCGCCC TCAACAGCAG CATAACTAAC 
CACATCAACA AACTGGATAA ATACGATTCC GTGGAGGCCA TCAAGGCATT TAACAAAAAC 
AACTTGATCC TAGAGGAGAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAC 
TCCAACGCCT ACTTTACAGA TCTCAACAAT TATGATGAAT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446322.1
CDS	3749..6973
Translation	MKGACILAWLFSSLGVWRLARPETQDPAKCQRAEHPVVSYKEIGPWLREFRAENAVDFSRLTFDPGQKELVVGARNYLFRLQLEDLSLIQAVQWECDEATKKACYSKGKSKEECQNYIRVLLVGGDRLFTCGTNAFTPVCTIRSLSNLTEIHDQISGMARCPYSPQHNSTALLTASGELYAATAMDFPGRDPAIYRSLGTLPPLRTAQYNSKWLNEPNFVSSYDIGNFTYFFFRENAVEHDCGKTVFSRAARVCKNDIGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQSTFFLPELDLIYGIFTTNVNSIAASAVCVFNLSAISQAFNGPFKYQENSRSAWLPYPNPNPNFQCGTMDQGLYVNLTERNLQDAQKFILMHEVVQPVTTVPSFMEDNSRFSHVAVDVVQGRDTLVHIIYLATDYGTIKKVRAPLSQSSGSCLLEEIELFPERKSEPIRSLKILHSQSVLFVGLQEHVVKIPLKRCHFHQTRGACIGAQDPYCGWDAVMKKCTSLEESLSMTQWDQSVPTCPTRNLTVDGSFGPWSPWTPCTHTDGTAVGSCLCRSRSCDSPAPQCGGWQCEGPRMEITNCSRNGGWTPWTSWSPCSTTCGIGFQVRQRSCSNPTPRHGGRVCVGQNREERYCNEHLLCPPHVFWTGWGPWERCTAQCGGGIQARRRTCENGPDCAGCNVEYQPCNTNACPELKKTTPWTPWTPVNISDNGGHYEQRFRYTCKARLPDPNLLEVGRQRIEMRYCSSDGTSGCSTDGLSGDFLRAGRYSAHTVNGAWSAWTSWSQCSRDCSRGIRNRKRVCNNPEPKYGGMPCLGPSLEFQECNILPCPVDGVWSCWSSWSKCSATCGGGHYMRTRSCTNPAPAYGGDICLGLHTEEALCNTQTCPENWSEWSEWSVCDASGTQVRTRQCILLFPVGSQCSGNTTESRPCVFDSNFIPEVSVARSSSVEEKRCGEFNMFHMMAVGLSSSILGCLLTLLVYTYCQRYQQQSHDATVIHPVSPAALNSSITNHINKLDKYDSVEAIKAFNKNNLILEERNKYFNPHLTGKTYSNAYFTDLNNYDEY

Target ORF information:

RefSeq Version	XM_017590833.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus semaphorin 5A (Sema5a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017590833.1

ORF Insert Sequence:

ATGAAGGGAG CCTGCATCCT TGCATGGCTG TTCTCAAGCC TGGGAGTGTG GAGACTTGCT 
CGGCCTGAGA CCCAGGACCC TGCCAAGTGC CAGAGAGCTG AGCACCCCGT CGTCTCCTAC 
AAAGAAATTG GCCCCTGGTT ACGGGAATTC AGAGCCGAGA ATGCTGTGGA TTTCTCGAGG 
TTAACATTTG ACCCAGGACA GAAAGAACTT GTCGTAGGAG CAAGAAACTA TCTCTTCAGA 
CTACAGCTCG AGGATCTGTC TCTCATCCAG GCTGTGCAGT GGGAGTGTGA TGAAGCCACG 
AAGAAGGCCT GTTACAGCAA GGGCAAATCG AAGGAGGAAT GTCAGAACTA CATACGAGTG 
CTCTTAGTGG GCGGGGACCG GCTCTTCACC TGTGGGACCA ATGCCTTCAC ACCCGTCTGT 
ACCATCCGCT CCTTAAGTAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCTCGA 
TGTCCCTATA GTCCCCAGCA CAATTCTACA GCTCTACTCA CTGCCAGTGG CGAGCTCTAT 
GCTGCAACAG CCATGGATTT CCCAGGGCGC GATCCAGCCA TTTATCGAAG TCTGGGCACT 
TTACCTCCTC TTCGAACTGC ACAGTACAAC TCCAAATGGC TCAATGAACC AAACTTTGTG 
TCTTCCTACG ACATCGGGAA CTTCACCTAC TTCTTCTTCC GAGAAAATGC CGTGGAACAC 
GACTGTGGGA AGACTGTGTT CTCCAGGGCA GCCAGGGTCT GCAAGAATGA CATTGGAGGA 
CGATTTCTTC TGGAGGATAC CTGGACTACC TTCATGAAGG CTCGCCTCAA CTGCTCTCGG 
CCTGGTGAGG TGCCGTTCTA CTACAACGAG CTTCAAAGCA CCTTCTTCCT GCCGGAGCTG 
GATCTGATCT ACGGCATCTT CACCACCAAT GTAAACAGCA TTGCTGCCTC TGCTGTCTGT 
GTCTTCAACC TGAGCGCCAT CTCACAGGCC TTCAATGGAC CCTTCAAGTA CCAAGAGAAC 
TCTCGCTCAG CCTGGCTGCC TTATCCCAAC CCCAACCCCA ATTTTCAGTG CGGCACCATG 
GACCAGGGCC TGTATGTAAA CCTGACGGAA AGAAACCTAC AGGATGCTCA GAAGTTCATC 
CTGATGCATG AAGTGGTGCA GCCAGTGACC ACAGTGCCTT CCTTCATGGA AGACAACAGC 
CGTTTCTCCC ATGTGGCTGT CGATGTTGTG CAAGGCAGGG ACACCCTTGT CCATATCATC 
TATCTGGCCA CAGATTATGG CACCATTAAA AAAGTGCGGG CTCCGCTGAG TCAGAGCTCG 
GGCAGCTGTT TGCTAGAAGA GATTGAGCTT TTTCCAGAGA GGAAGAGTGA GCCCATCAGG 
AGCCTAAAGA TCCTCCACAG CCAGAGTGTC CTATTTGTGG GGCTACAGGA GCATGTGGTC 
AAGATCCCCC TGAAGAGATG CCATTTTCAT CAAACACGTG GTGCCTGCAT TGGTGCTCAG 
GACCCTTACT GTGGCTGGGA TGCGGTGATG AAGAAGTGCA CCAGCCTGGA GGAGAGCCTG 
AGCATGACCC AGTGGGATCA GAGCGTCCCC ACCTGTCCGA CCAGAAATCT CACCGTAGAT 
GGGAGCTTTG GCCCGTGGTC TCCATGGACA CCCTGTACTC ACACTGATGG CACTGCTGTG 
GGATCCTGCC TCTGCCGGTC CCGATCCTGT GACAGCCCAG CTCCACAGTG TGGCGGCTGG 
CAGTGTGAGG GCCCTAGAAT GGAGATCACC AACTGTTCCA GGAATGGAGG CTGGACTCCC 
TGGACCTCCT GGTCTCCCTG CAGCACCACT TGTGGCATTG GCTTCCAGGT GCGGCAGCGC 
TCCTGCAGCA ACCCTACGCC CAGGCATGGA GGGCGTGTGT GCGTGGGCCA GAACAGGGAG 
GAAAGATACT GCAATGAACA TTTACTCTGC CCACCACACG TGTTCTGGAC AGGCTGGGGA 
CCTTGGGAAC GCTGCACAGC CCAGTGCGGG GGTGGCATTC AAGCTCGCCG TAGGACCTGT 
GAAAATGGGC CTGACTGTGC AGGATGCAAT GTGGAATACC AGCCTTGTAA CACCAATGCA 
TGCCCAGAGT TGAAGAAGAC CACACCATGG ACCCCCTGGA CACCTGTCAA CATCTCTGAC 
AATGGGGGCC ACTATGAGCA GCGGTTCCGC TACACCTGTA AAGCTCGCCT GCCAGATCCA 
AACTTATTGG AAGTAGGAAG ACAAAGGATA GAAATGCGGT ACTGTTCTAG TGATGGGACC 
AGTGGCTGCT CCACAGATGG ACTTTCTGGA GACTTTCTAA GAGCTGGGAG ATACTCTGCC 
CATACAGTCA ATGGGGCATG GTCAGCCTGG ACTTCCTGGT CACAGTGCAG CAGAGACTGC 
AGCAGGGGCA TTCGAAACAG GAAGCGTGTT TGCAACAACC CAGAGCCCAA GTATGGAGGT 
ATGCCATGCC TTGGTCCATC ACTGGAGTTC CAGGAATGCA ACATTTTACC CTGTCCAGTG 
GATGGTGTGT GGTCTTGCTG GTCATCCTGG TCTAAATGCT CAGCAACTTG TGGGGGTGGA 
CACTACATGA GAACCCGTTC GTGTACGAAT CCAGCCCCAG CATACGGAGG GGACATCTGC 
CTGGGACTGC ACACGGAAGA GGCCCTCTGT AACACACAGA CCTGCCCAGA GAACTGGTCA 
GAGTGGTCAG AGTGGTCTGT GTGTGATGCA TCTGGTACCC AGGTCCGTAC TCGGCAGTGC 
ATCCTTCTGT TTCCTGTGGG CAGCCAGTGT TCTGGAAATA CTACAGAGAG CCGGCCTTGT 
GTATTTGACT CTAATTTCAT CCCAGAAGTG TCTGTGGCAA GATCCAGCAG CGTAGAAGAG 
AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATGGCTG TGGGACTTAG CAGTTCCATC 
CTCGGCTGCC TCCTTACACT GCTTGTCTAC ACCTACTGCC AGCGGTACCA GCAGCAGTCC 
CATGATGCAA CTGTCATCCA TCCTGTTTCT CCCGCCGCCC TCAACAGCAG CATAACTAAC 
CACATCAACA AACTGGATAA ATACGATTCC GTGGAGGCCA TCAAGGCATT TAACAAAAAC 
AACTTGATCC TAGAGGAGAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAC 
TCCAACGCCT ACTTTACAGA TCTCAACAAT TATGATGAAT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Class 5 transmembrane semaphorins control selective Mammalian retinal lamination and function.
Neuron71(3)460-73(2011 Aug)
Matsuoka RL,Chivatakarn O,Badea TC,Samuels IS,Cahill H,Katayama K,Kumar SR,Suto F,Ch?dotal A,Peachey NS,Nathans J,Yoshida Y,Giger RJ,Kolodkin AL

Semaphorin 5A promotes angiogenesis by increasing endothelial cell proliferation, migration, and decreasing apoptosis.
Microvascular research79(1)1-9(2010 Jan)
Sadanandam A,Rosenbaugh EG,Singh S,Varney M,Singh RK

Semaphorin5A expression in the developing chick telencephalon.
Brain research bulletin66(4-6)436-40(2005 Sep)
Pineda D,Garc?a B,Olmos JL,D?vila JC,Real MA,Guirado S

Inactivation of the Sema5a gene results in embryonic lethality and defective remodeling of the cranial vascular system.
Molecular and cellular biology25(6)2310-9(2005 Mar)
Fiore R,Rahim B,Christoffels VM,Moorman AF,P?schel AW

Semaphorin 5A is a bifunctional axon guidance cue regulated by heparan and chondroitin sulfate proteoglycans.
Neuron44(6)961-75(2004 Dec)
Kantor DB,Chivatakarn O,Peer KL,Oster SF,Inatani M,Hansen MJ,Flanagan JG,Yamaguchi Y,Sretavan DW,Giger RJ,Kolodkin AL

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Sema5a cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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