Topbp1 cDNA ORF clone, Rattus norvegicus(Norway rat)

The following Topbp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Topbp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa47492 XM_008757856.2
Latest version!
Rattus norvegicus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
ORa47492 XM_006243684.2
Latest version!
Rattus norvegicus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
ORa47492 XM_008766589.2
Latest version!
Rattus norvegicus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
ORa18094 XM_006226542.2
Latest version!
Rattus norvegicus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
ORa47491 XM_236578.8
Latest version!
Rattus norvegicus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
ORa47491 XM_001072207.5
Latest version!
Rattus norvegicus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
ORa47491 XM_008757855.2
Latest version!
Rattus norvegicus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ORa47492
    Clone ID Related Accession (Same CDS sequence) XM_008757856.2 , XM_006243684.2 , XM_008766589.2
    Accession Version XM_008757856.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3987bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product DNA topoisomerase 2-binding protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000076.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008757856.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCCAGAA ATGACCAAGA GCCGTTTTTT GTGAAGTTTT TAAAGTCTTC GGACAATTCA 
    GAATGTTTTT TTAAAGCTCT TGAGTCAATA AAGGAGTTAC AATCAGAAGA GTATCTTCAG
    GTTATTACCG ATGAGGAAGC ATTGAGGATT AAAGAGAATG ACAAATCGCT TTATATCTGT
    GACCGTTTCA GTGGCACTGC TTTTGATCAC CTTAAACAGC TTGGCTGCAG AATTGTTGGT
    CCTCAAGTAG TCACATTTTG TATGCGCCAC CAGCAGTGTG TCCCAAGAGC TGAACATCCA
    GTTTATAATA TGATTATGTC TGACATAACT ATATCTTGTA CAAGCCTGGA TAAAGACAAG
    AGGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT
    GTCTCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAATACTT AGTTGCTGCT
    AATCTGAAGA AACCTATTTT GCTTCCTGCT TGGATTAAAA CACTTTGGGA GAAGTCACAA
    GAAAAAAAAA TAACCAAATA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTC
    CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGTATAC ACAGGAAGAC AGTCCAGCAG
    CTCACAGCTA AGCATGGAGG CCAGTACATG GGGCAGCTGA AGATGAACGA GTGCACACAC
    CTCATTGTGC AAGAACCAAA AGGACAAAAA TATGAATGTG CCAGGAGATG GAATGTGCAC
    TGTGTGACCC TGCAATGGTT CCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAGTCT
    ATATACAAGG CCGAACCCAG AGCAGCGGTA AAGACGGTGC CTGATACTTC CACACCCACT
    GCTCAGAGCG GCAGTGCTGC AGCTGAGAAT CACAGGCTGG CAGATGTCAG CCATATATCC
    AACATCAACG GCAGCTGTGT AAATGAAACA ATGTTTGGTT CAACTACGAG CAAACTCGAA
    TGCACACTTG AGAACCTAGA AAATCTGGAC ATCAGTATGT TTCAAGCACC TGAAGATCTA
    TTAGATGGCT GTCGGATATA TCTTTGTGGC TTTAGTGGCA GAAAGCTCGA TAAGTTGAGA
    AGGCTTATTA ACAGTGGAGG TGGAGTTCGG TTTAATCAAC TCAATGAAGA TGTAACTCAT
    GTAATCATGG GAGATTATGA GGATGACGTG AGGCAGTTTT GGAGCAAATC CTCCCACAGG
    CCCCATGTAG TGGGAGCAAA GTGGTTGCTG GAGTGTTTTA GTAAAGGATA TATACTTCCT
    GAAGAGTCAT ATATCCACGC TAACTACCAG CCAGATGGAA TTCCAGTTTC AGATCAGCCC
    AGAAACCAGA CAACTGTCTT AGAAAAGAGT GGCACATTCT CTAAGAAGGA CTTTGTTCCT
    AATGAGAAGT TTCAGCAGAC TGATGAGGAT CTGCTTGCTC AGTATGTACA TGATGACTCC
    ACAATAGTTG AAGCTAAGCT ATCTGAAGCT ATAGAGCCTG AAGTTGGGCC TTGCGGTGGT
    TCTGCTCATG TTGAGCCCTG TGATGATTCC ACTCACATTT CTGTGCAAGA GGAAAACAAG
    TCGTCTGTCA GTCATTGCCT CCTTGATGGC TCTACAGTTC CTGAGGAAGG CTTATTTAGC
    CAAAAGAGTT TCCTTGTTTT GGGTTTTAGT GTTGAAAATG AATGTAATAT TGTAAACATC
    ATAAGAGAGC ATGCTGGAAA GATTGTGTCC CTTCCCAGCA GAATTGTTGC TGACTATGCC
    GTGGTTCCCC TGCTGGGATG TGAAGTAGAA GCGACAGTGG CAGAAGTTGT TACAAACACA
    TGGCTGGTTA CATGCATAGA CAACCAGACA TTAGTTGATC CCAAGTCGAA TCCCCTCTTC
    ACGCCAGTCT CAGTAATGTC AGGAATGACT CCTTTAGAGG ACTGTGTGAT TTCCTTCAGT
    CAGTGTGTGG GAGCAGAAAG AGATTCCTTG GTATTCTTAG CAAATCATCT TGGAGCAAGT
    GTTCAAGAAT TCTTTGTCCG CAAATCCAAT GCAAAGAAAG GCATGCTCGC CAGTACACAT
    CTTATAGTGA AAGAACCGAC TGGTTCTAAA TATGAAGCTG CAAAGAAATG GAGTTTGCCA
    GCAGTTAATA TTTCGTGGCT CTTAGAAACT GCTAGAATGG GGAAGAGAGC AGATGAAAAC
    CATTTTCTGG TTGACAGTGC ACCTAAACAA GAACAAGTAT TAGAAACTAA CATACCAAAT
    GGAGTGAGTC CAAATCCAGA TTCTCCAGCA CATCCTGATG CACACTTGGA AATTCACAGA
    CAAAAGGCCG TTACACCTTT GGACATGAAC CGATTTCAGA GTAAAGCTTT CCATGCTGTC
    ATCTCACAGC ACAACAGGCA GGGCTTGGCC TCCCCACCAG TGAGACAGCC ACCTAAGAAG
    GAACCCTCCT TCCACCTGGA CACACCCTCA AAGTTCCTGT CCAAGGACAA GCTTTTCAAG
    CCATCCTTTG ATGTGACGGA TGCACTTGCA GCCTTAGAAA CCCCAAATGC TGCCAGCCAG
    AAGCGGAAAC TGAGCTCACC ACTCTCTGAA GTCATCGTCA GAAACCTGAC AACTGCTTTG
    GCAAATAGTT CACGAAACAC CGGCTGTCTT TCTGCTAGCC CTCAGCTAAA GGGTGCCCAT
    TTGGAGAAGG AAGAGACTCC GAAACCGCTT GACAAAGTTG TGGTGTGCGT GAGTAAAAGG
    CTCAGTAAGA AGCAGAGTGA GCTAAATGGG GTTGCAGCCT CACTGGGAGC AGAGTACAGG
    TGGAGTTTTG ATGAGACAGT TACTCATTAC ATTTACCAGG GTCGGGCAAA TGATAGTAAT
    CGCGAGTATA AATCTGCAAA GGAAAGAGGG GTGCACGTCG TGTCGGAGCA TTGGCTGTTA
    GAGTGTGCTC AAGAGTATAA GCATCTTCCA GAATCTCTTT ACCCACACAC TTACAACCCC
    AAAATGAGCT TGGACATCAG CACAGTGCAG GACGGGCGGC TCTGTGACAG CCGCTTACCC
    TCGGCACTTT CTGCATCTAA GGACGATGAG CCAGATCACC TGTCTGTAGA AGAAAATGCA
    ACAGAGACTT TGGGCACACA CAGCAGAGAG TCCGCTCTGC TGAATGGGAA TGGAAGGGAC
    GACTGCAAAG GAGCTCTGAC CCAGGCCTTG GAGATGAGAG AGAACTTCCA GAAACAACTA
    CAAGAGATCA TGTCAGCAAC CTCCATCGTG AGGACACCAG CCCAGAAGAC TTGCATGTCA
    AGAAGTAGTT GTAACAGCGC CTCTTCAACT CCTGACAGCA CTCGCTCTGC TCGGAGTGGA
    CGAAGCCGAG TCTTGGAGGC GCTCAGGCAG TCTCGACAGA TAGTGCCTGA TATCAACACA
    GAGCCCTCCC AGAATGAACA GATCATTTGG GATGACCCAA CAGCAAGGGA GGAGAGAGCA
    AGGCTTGCCA GCAACCTACA GTGGCCTAGC TACCCAACAC AGTATTCCGA GCTTCAGGTT
    GAGATAAAAA AACTGGATGA TTCTCCTTCT CGAAAGCCTG TGTACCATTC AGAAATTGCT
    GGGCAGGCCT CGTGTGTGAC TCAAGCCCCA GGGCAGCACC CAGGCTCTGA AGAACCAGAA
    GCTCCAGCAG CAGAAAGGAA TCTGATCCCT GAGCCTCAAG CCCCCAGCGT GGCTTTCCCC
    CTGGCTAAGC CGCCTGTAGC TCCACAACCT GAGGACAAGA TTGAAACACT GGAGGAGACG
    CATGGAAAAC TTAAAAAACA GTATGTGTTC CAGATGTCAT CTCTGAATTC TCAAGAGCGG
    ATTGATTACT GTCGCCTGAT TAAAGACCTA GGCCCGGGAG TGCCCAGTGT GATAGTGCAC
    ACATGGAGCG CAGAACAGGT TCCGGGGAGG ACTCCCACCT GTCTTGGCCA AAATAAAAAG
    ATCCATGGAC TCTGCAGTCA GAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008756078.2
    CDS206..4192
    Translation

    Target ORF information:

    RefSeq Version XM_008757856.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008757856.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCCAGAA ATGACCAAGA GCCGTTTTTT GTGAAGTTTT TAAAGTCTTC GGACAATTCA 
    GAATGTTTTT TTAAAGCTCT TGAGTCAATA AAGGAGTTAC AATCAGAAGA GTATCTTCAG
    GTTATTACCG ATGAGGAAGC ATTGAGGATT AAAGAGAATG ACAAATCGCT TTATATCTGT
    GACCGTTTCA GTGGCACTGC TTTTGATCAC CTTAAACAGC TTGGCTGCAG AATTGTTGGT
    CCTCAAGTAG TCACATTTTG TATGCGCCAC CAGCAGTGTG TCCCAAGAGC TGAACATCCA
    GTTTATAATA TGATTATGTC TGACATAACT ATATCTTGTA CAAGCCTGGA TAAAGACAAG
    AGGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT
    GTCTCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAATACTT AGTTGCTGCT
    AATCTGAAGA AACCTATTTT GCTTCCTGCT TGGATTAAAA CACTTTGGGA GAAGTCACAA
    GAAAAAAAAA TAACCAAATA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTC
    CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGTATAC ACAGGAAGAC AGTCCAGCAG
    CTCACAGCTA AGCATGGAGG CCAGTACATG GGGCAGCTGA AGATGAACGA GTGCACACAC
    CTCATTGTGC AAGAACCAAA AGGACAAAAA TATGAATGTG CCAGGAGATG GAATGTGCAC
    TGTGTGACCC TGCAATGGTT CCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAGTCT
    ATATACAAGG CCGAACCCAG AGCAGCGGTA AAGACGGTGC CTGATACTTC CACACCCACT
    GCTCAGAGCG GCAGTGCTGC AGCTGAGAAT CACAGGCTGG CAGATGTCAG CCATATATCC
    AACATCAACG GCAGCTGTGT AAATGAAACA ATGTTTGGTT CAACTACGAG CAAACTCGAA
    TGCACACTTG AGAACCTAGA AAATCTGGAC ATCAGTATGT TTCAAGCACC TGAAGATCTA
    TTAGATGGCT GTCGGATATA TCTTTGTGGC TTTAGTGGCA GAAAGCTCGA TAAGTTGAGA
    AGGCTTATTA ACAGTGGAGG TGGAGTTCGG TTTAATCAAC TCAATGAAGA TGTAACTCAT
    GTAATCATGG GAGATTATGA GGATGACGTG AGGCAGTTTT GGAGCAAATC CTCCCACAGG
    CCCCATGTAG TGGGAGCAAA GTGGTTGCTG GAGTGTTTTA GTAAAGGATA TATACTTCCT
    GAAGAGTCAT ATATCCACGC TAACTACCAG CCAGATGGAA TTCCAGTTTC AGATCAGCCC
    AGAAACCAGA CAACTGTCTT AGAAAAGAGT GGCACATTCT CTAAGAAGGA CTTTGTTCCT
    AATGAGAAGT TTCAGCAGAC TGATGAGGAT CTGCTTGCTC AGTATGTACA TGATGACTCC
    ACAATAGTTG AAGCTAAGCT ATCTGAAGCT ATAGAGCCTG AAGTTGGGCC TTGCGGTGGT
    TCTGCTCATG TTGAGCCCTG TGATGATTCC ACTCACATTT CTGTGCAAGA GGAAAACAAG
    TCGTCTGTCA GTCATTGCCT CCTTGATGGC TCTACAGTTC CTGAGGAAGG CTTATTTAGC
    CAAAAGAGTT TCCTTGTTTT GGGTTTTAGT GTTGAAAATG AATGTAATAT TGTAAACATC
    ATAAGAGAGC ATGCTGGAAA GATTGTGTCC CTTCCCAGCA GAATTGTTGC TGACTATGCC
    GTGGTTCCCC TGCTGGGATG TGAAGTAGAA GCGACAGTGG CAGAAGTTGT TACAAACACA
    TGGCTGGTTA CATGCATAGA CAACCAGACA TTAGTTGATC CCAAGTCGAA TCCCCTCTTC
    ACGCCAGTCT CAGTAATGTC AGGAATGACT CCTTTAGAGG ACTGTGTGAT TTCCTTCAGT
    CAGTGTGTGG GAGCAGAAAG AGATTCCTTG GTATTCTTAG CAAATCATCT TGGAGCAAGT
    GTTCAAGAAT TCTTTGTCCG CAAATCCAAT GCAAAGAAAG GCATGCTCGC CAGTACACAT
    CTTATAGTGA AAGAACCGAC TGGTTCTAAA TATGAAGCTG CAAAGAAATG GAGTTTGCCA
    GCAGTTAATA TTTCGTGGCT CTTAGAAACT GCTAGAATGG GGAAGAGAGC AGATGAAAAC
    CATTTTCTGG TTGACAGTGC ACCTAAACAA GAACAAGTAT TAGAAACTAA CATACCAAAT
    GGAGTGAGTC CAAATCCAGA TTCTCCAGCA CATCCTGATG CACACTTGGA AATTCACAGA
    CAAAAGGCCG TTACACCTTT GGACATGAAC CGATTTCAGA GTAAAGCTTT CCATGCTGTC
    ATCTCACAGC ACAACAGGCA GGGCTTGGCC TCCCCACCAG TGAGACAGCC ACCTAAGAAG
    GAACCCTCCT TCCACCTGGA CACACCCTCA AAGTTCCTGT CCAAGGACAA GCTTTTCAAG
    CCATCCTTTG ATGTGACGGA TGCACTTGCA GCCTTAGAAA CCCCAAATGC TGCCAGCCAG
    AAGCGGAAAC TGAGCTCACC ACTCTCTGAA GTCATCGTCA GAAACCTGAC AACTGCTTTG
    GCAAATAGTT CACGAAACAC CGGCTGTCTT TCTGCTAGCC CTCAGCTAAA GGGTGCCCAT
    TTGGAGAAGG AAGAGACTCC GAAACCGCTT GACAAAGTTG TGGTGTGCGT GAGTAAAAGG
    CTCAGTAAGA AGCAGAGTGA GCTAAATGGG GTTGCAGCCT CACTGGGAGC AGAGTACAGG
    TGGAGTTTTG ATGAGACAGT TACTCATTAC ATTTACCAGG GTCGGGCAAA TGATAGTAAT
    CGCGAGTATA AATCTGCAAA GGAAAGAGGG GTGCACGTCG TGTCGGAGCA TTGGCTGTTA
    GAGTGTGCTC AAGAGTATAA GCATCTTCCA GAATCTCTTT ACCCACACAC TTACAACCCC
    AAAATGAGCT TGGACATCAG CACAGTGCAG GACGGGCGGC TCTGTGACAG CCGCTTACCC
    TCGGCACTTT CTGCATCTAA GGACGATGAG CCAGATCACC TGTCTGTAGA AGAAAATGCA
    ACAGAGACTT TGGGCACACA CAGCAGAGAG TCCGCTCTGC TGAATGGGAA TGGAAGGGAC
    GACTGCAAAG GAGCTCTGAC CCAGGCCTTG GAGATGAGAG AGAACTTCCA GAAACAACTA
    CAAGAGATCA TGTCAGCAAC CTCCATCGTG AGGACACCAG CCCAGAAGAC TTGCATGTCA
    AGAAGTAGTT GTAACAGCGC CTCTTCAACT CCTGACAGCA CTCGCTCTGC TCGGAGTGGA
    CGAAGCCGAG TCTTGGAGGC GCTCAGGCAG TCTCGACAGA TAGTGCCTGA TATCAACACA
    GAGCCCTCCC AGAATGAACA GATCATTTGG GATGACCCAA CAGCAAGGGA GGAGAGAGCA
    AGGCTTGCCA GCAACCTACA GTGGCCTAGC TACCCAACAC AGTATTCCGA GCTTCAGGTT
    GAGATAAAAA AACTGGATGA TTCTCCTTCT CGAAAGCCTG TGTACCATTC AGAAATTGCT
    GGGCAGGCCT CGTGTGTGAC TCAAGCCCCA GGGCAGCACC CAGGCTCTGA AGAACCAGAA
    GCTCCAGCAG CAGAAAGGAA TCTGATCCCT GAGCCTCAAG CCCCCAGCGT GGCTTTCCCC
    CTGGCTAAGC CGCCTGTAGC TCCACAACCT GAGGACAAGA TTGAAACACT GGAGGAGACG
    CATGGAAAAC TTAAAAAACA GTATGTGTTC CAGATGTCAT CTCTGAATTC TCAAGAGCGG
    ATTGATTACT GTCGCCTGAT TAAAGACCTA GGCCCGGGAG TGCCCAGTGT GATAGTGCAC
    ACATGGAGCG CAGAACAGGT TCCGGGGAGG ACTCCCACCT GTCTTGGCCA AAATAAAAAG
    ATCCATGGAC TCTGCAGTCA GAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa47492
    Clone ID Related Accession (Same CDS sequence) XM_008757856.2 , XM_006243684.2 , XM_008766589.2
    Accession Version XM_006243684.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3987bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product DNA topoisomerase 2-binding protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006243684.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCCAGAA ATGACCAAGA GCCGTTTTTT GTGAAGTTTT TAAAGTCTTC GGACAATTCA 
    GAATGTTTTT TTAAAGCTCT TGAGTCAATA AAGGAGTTAC AATCAGAAGA GTATCTTCAG
    GTTATTACCG ATGAGGAAGC ATTGAGGATT AAAGAGAATG ACAAATCGCT TTATATCTGT
    GACCGTTTCA GTGGCACTGC TTTTGATCAC CTTAAACAGC TTGGCTGCAG AATTGTTGGT
    CCTCAAGTAG TCACATTTTG TATGCGCCAC CAGCAGTGTG TCCCAAGAGC TGAACATCCA
    GTTTATAATA TGATTATGTC TGACATAACT ATATCTTGTA CAAGCCTGGA TAAAGACAAG
    AGGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT
    GTCTCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAATACTT AGTTGCTGCT
    AATCTGAAGA AACCTATTTT GCTTCCTGCT TGGATTAAAA CACTTTGGGA GAAGTCACAA
    GAAAAAAAAA TAACCAAATA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTC
    CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGTATAC ACAGGAAGAC AGTCCAGCAG
    CTCACAGCTA AGCATGGAGG CCAGTACATG GGGCAGCTGA AGATGAACGA GTGCACACAC
    CTCATTGTGC AAGAACCAAA AGGACAAAAA TATGAATGTG CCAGGAGATG GAATGTGCAC
    TGTGTGACCC TGCAATGGTT CCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAGTCT
    ATATACAAGG CCGAACCCAG AGCAGCGGTA AAGACGGTGC CTGATACTTC CACACCCACT
    GCTCAGAGCG GCAGTGCTGC AGCTGAGAAT CACAGGCTGG CAGATGTCAG CCATATATCC
    AACATCAACG GCAGCTGTGT AAATGAAACA ATGTTTGGTT CAACTACGAG CAAACTCGAA
    TGCACACTTG AGAACCTAGA AAATCTGGAC ATCAGTATGT TTCAAGCACC TGAAGATCTA
    TTAGATGGCT GTCGGATATA TCTTTGTGGC TTTAGTGGCA GAAAGCTCGA TAAGTTGAGA
    AGGCTTATTA ACAGTGGAGG TGGAGTTCGG TTTAATCAAC TCAATGAAGA TGTAACTCAT
    GTAATCATGG GAGATTATGA GGATGACGTG AGGCAGTTTT GGAGCAAATC CTCCCACAGG
    CCCCATGTAG TGGGAGCAAA GTGGTTGCTG GAGTGTTTTA GTAAAGGATA TATACTTCCT
    GAAGAGTCAT ATATCCACGC TAACTACCAG CCAGATGGAA TTCCAGTTTC AGATCAGCCC
    AGAAACCAGA CAACTGTCTT AGAAAAGAGT GGCACATTCT CTAAGAAGGA CTTTGTTCCT
    AATGAGAAGT TTCAGCAGAC TGATGAGGAT CTGCTTGCTC AGTATGTACA TGATGACTCC
    ACAATAGTTG AAGCTAAGCT ATCTGAAGCT ATAGAGCCTG AAGTTGGGCC TTGCGGTGGT
    TCTGCTCATG TTGAGCCCTG TGATGATTCC ACTCACATTT CTGTGCAAGA GGAAAACAAG
    TCGTCTGTCA GTCATTGCCT CCTTGATGGC TCTACAGTTC CTGAGGAAGG CTTATTTAGC
    CAAAAGAGTT TCCTTGTTTT GGGTTTTAGT GTTGAAAATG AATGTAATAT TGTAAACATC
    ATAAGAGAGC ATGCTGGAAA GATTGTGTCC CTTCCCAGCA GAATTGTTGC TGACTATGCC
    GTGGTTCCCC TGCTGGGATG TGAAGTAGAA GCGACAGTGG CAGAAGTTGT TACAAACACA
    TGGCTGGTTA CATGCATAGA CAACCAGACA TTAGTTGATC CCAAGTCGAA TCCCCTCTTC
    ACGCCAGTCT CAGTAATGTC AGGAATGACT CCTTTAGAGG ACTGTGTGAT TTCCTTCAGT
    CAGTGTGTGG GAGCAGAAAG AGATTCCTTG GTATTCTTAG CAAATCATCT TGGAGCAAGT
    GTTCAAGAAT TCTTTGTCCG CAAATCCAAT GCAAAGAAAG GCATGCTCGC CAGTACACAT
    CTTATAGTGA AAGAACCGAC TGGTTCTAAA TATGAAGCTG CAAAGAAATG GAGTTTGCCA
    GCAGTTAATA TTTCGTGGCT CTTAGAAACT GCTAGAATGG GGAAGAGAGC AGATGAAAAC
    CATTTTCTGG TTGACAGTGC ACCTAAACAA GAACAAGTAT TAGAAACTAA CATACCAAAT
    GGAGTGAGTC CAAATCCAGA TTCTCCAGCA CATCCTGATG CACACTTGGA AATTCACAGA
    CAAAAGGCCG TTACACCTTT GGACATGAAC CGATTTCAGA GTAAAGCTTT CCATGCTGTC
    ATCTCACAGC ACAACAGGCA GGGCTTGGCC TCCCCACCAG TGAGACAGCC ACCTAAGAAG
    GAACCCTCCT TCCACCTGGA CACACCCTCA AAGTTCCTGT CCAAGGACAA GCTTTTCAAG
    CCATCCTTTG ATGTGACGGA TGCACTTGCA GCCTTAGAAA CCCCAAATGC TGCCAGCCAG
    AAGCGGAAAC TGAGCTCACC ACTCTCTGAA GTCATCGTCA GAAACCTGAC AACTGCTTTG
    GCAAATAGTT CACGAAACAC CGGCTGTCTT TCTGCTAGCC CTCAGCTAAA GGGTGCCCAT
    TTGGAGAAGG AAGAGACTCC GAAACCGCTT GACAAAGTTG TGGTGTGCGT GAGTAAAAGG
    CTCAGTAAGA AGCAGAGTGA GCTAAATGGG GTTGCAGCCT CACTGGGAGC AGAGTACAGG
    TGGAGTTTTG ATGAGACAGT TACTCATTAC ATTTACCAGG GTCGGGCAAA TGATAGTAAT
    CGCGAGTATA AATCTGCAAA GGAAAGAGGG GTGCACGTCG TGTCGGAGCA TTGGCTGTTA
    GAGTGTGCTC AAGAGTATAA GCATCTTCCA GAATCTCTTT ACCCACACAC TTACAACCCC
    AAAATGAGCT TGGACATCAG CACAGTGCAG GACGGGCGGC TCTGTGACAG CCGCTTACCC
    TCGGCACTTT CTGCATCTAA GGACGATGAG CCAGATCACC TGTCTGTAGA AGAAAATGCA
    ACAGAGACTT TGGGCACACA CAGCAGAGAG TCCGCTCTGC TGAATGGGAA TGGAAGGGAC
    GACTGCAAAG GAGCTCTGAC CCAGGCCTTG GAGATGAGAG AGAACTTCCA GAAACAACTA
    CAAGAGATCA TGTCAGCAAC CTCCATCGTG AGGACACCAG CCCAGAAGAC TTGCATGTCA
    AGAAGTAGTT GTAACAGCGC CTCTTCAACT CCTGACAGCA CTCGCTCTGC TCGGAGTGGA
    CGAAGCCGAG TCTTGGAGGC GCTCAGGCAG TCTCGACAGA TAGTGCCTGA TATCAACACA
    GAGCCCTCCC AGAATGAACA GATCATTTGG GATGACCCAA CAGCAAGGGA GGAGAGAGCA
    AGGCTTGCCA GCAACCTACA GTGGCCTAGC TACCCAACAC AGTATTCCGA GCTTCAGGTT
    GAGATAAAAA AACTGGATGA TTCTCCTTCT CGAAAGCCTG TGTACCATTC AGAAATTGCT
    GGGCAGGCCT CGTGTGTGAC TCAAGCCCCA GGGCAGCACC CAGGCTCTGA AGAACCAGAA
    GCTCCAGCAG CAGAAAGGAA TCTGATCCCT GAGCCTCAAG CCCCCAGCGT GGCTTTCCCC
    CTGGCTAAGC CGCCTGTAGC TCCACAACCT GAGGACAAGA TTGAAACACT GGAGGAGACG
    CATGGAAAAC TTAAAAAACA GTATGTGTTC CAGATGTCAT CTCTGAATTC TCAAGAGCGG
    ATTGATTACT GTCGCCTGAT TAAAGACCTA GGCCCGGGAG TGCCCAGTGT GATAGTGCAC
    ACATGGAGCG CAGAACAGGT TCCGGGGAGG ACTCCCACCT GTCTTGGCCA AAATAAAAAG
    ATCCATGGAC TCTGCAGTCA GAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006243746.2
    CDS206..4192
    Translation

    Target ORF information:

    RefSeq Version XM_006243684.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006243684.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCCAGAA ATGACCAAGA GCCGTTTTTT GTGAAGTTTT TAAAGTCTTC GGACAATTCA 
    GAATGTTTTT TTAAAGCTCT TGAGTCAATA AAGGAGTTAC AATCAGAAGA GTATCTTCAG
    GTTATTACCG ATGAGGAAGC ATTGAGGATT AAAGAGAATG ACAAATCGCT TTATATCTGT
    GACCGTTTCA GTGGCACTGC TTTTGATCAC CTTAAACAGC TTGGCTGCAG AATTGTTGGT
    CCTCAAGTAG TCACATTTTG TATGCGCCAC CAGCAGTGTG TCCCAAGAGC TGAACATCCA
    GTTTATAATA TGATTATGTC TGACATAACT ATATCTTGTA CAAGCCTGGA TAAAGACAAG
    AGGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT
    GTCTCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAATACTT AGTTGCTGCT
    AATCTGAAGA AACCTATTTT GCTTCCTGCT TGGATTAAAA CACTTTGGGA GAAGTCACAA
    GAAAAAAAAA TAACCAAATA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTC
    CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGTATAC ACAGGAAGAC AGTCCAGCAG
    CTCACAGCTA AGCATGGAGG CCAGTACATG GGGCAGCTGA AGATGAACGA GTGCACACAC
    CTCATTGTGC AAGAACCAAA AGGACAAAAA TATGAATGTG CCAGGAGATG GAATGTGCAC
    TGTGTGACCC TGCAATGGTT CCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAGTCT
    ATATACAAGG CCGAACCCAG AGCAGCGGTA AAGACGGTGC CTGATACTTC CACACCCACT
    GCTCAGAGCG GCAGTGCTGC AGCTGAGAAT CACAGGCTGG CAGATGTCAG CCATATATCC
    AACATCAACG GCAGCTGTGT AAATGAAACA ATGTTTGGTT CAACTACGAG CAAACTCGAA
    TGCACACTTG AGAACCTAGA AAATCTGGAC ATCAGTATGT TTCAAGCACC TGAAGATCTA
    TTAGATGGCT GTCGGATATA TCTTTGTGGC TTTAGTGGCA GAAAGCTCGA TAAGTTGAGA
    AGGCTTATTA ACAGTGGAGG TGGAGTTCGG TTTAATCAAC TCAATGAAGA TGTAACTCAT
    GTAATCATGG GAGATTATGA GGATGACGTG AGGCAGTTTT GGAGCAAATC CTCCCACAGG
    CCCCATGTAG TGGGAGCAAA GTGGTTGCTG GAGTGTTTTA GTAAAGGATA TATACTTCCT
    GAAGAGTCAT ATATCCACGC TAACTACCAG CCAGATGGAA TTCCAGTTTC AGATCAGCCC
    AGAAACCAGA CAACTGTCTT AGAAAAGAGT GGCACATTCT CTAAGAAGGA CTTTGTTCCT
    AATGAGAAGT TTCAGCAGAC TGATGAGGAT CTGCTTGCTC AGTATGTACA TGATGACTCC
    ACAATAGTTG AAGCTAAGCT ATCTGAAGCT ATAGAGCCTG AAGTTGGGCC TTGCGGTGGT
    TCTGCTCATG TTGAGCCCTG TGATGATTCC ACTCACATTT CTGTGCAAGA GGAAAACAAG
    TCGTCTGTCA GTCATTGCCT CCTTGATGGC TCTACAGTTC CTGAGGAAGG CTTATTTAGC
    CAAAAGAGTT TCCTTGTTTT GGGTTTTAGT GTTGAAAATG AATGTAATAT TGTAAACATC
    ATAAGAGAGC ATGCTGGAAA GATTGTGTCC CTTCCCAGCA GAATTGTTGC TGACTATGCC
    GTGGTTCCCC TGCTGGGATG TGAAGTAGAA GCGACAGTGG CAGAAGTTGT TACAAACACA
    TGGCTGGTTA CATGCATAGA CAACCAGACA TTAGTTGATC CCAAGTCGAA TCCCCTCTTC
    ACGCCAGTCT CAGTAATGTC AGGAATGACT CCTTTAGAGG ACTGTGTGAT TTCCTTCAGT
    CAGTGTGTGG GAGCAGAAAG AGATTCCTTG GTATTCTTAG CAAATCATCT TGGAGCAAGT
    GTTCAAGAAT TCTTTGTCCG CAAATCCAAT GCAAAGAAAG GCATGCTCGC CAGTACACAT
    CTTATAGTGA AAGAACCGAC TGGTTCTAAA TATGAAGCTG CAAAGAAATG GAGTTTGCCA
    GCAGTTAATA TTTCGTGGCT CTTAGAAACT GCTAGAATGG GGAAGAGAGC AGATGAAAAC
    CATTTTCTGG TTGACAGTGC ACCTAAACAA GAACAAGTAT TAGAAACTAA CATACCAAAT
    GGAGTGAGTC CAAATCCAGA TTCTCCAGCA CATCCTGATG CACACTTGGA AATTCACAGA
    CAAAAGGCCG TTACACCTTT GGACATGAAC CGATTTCAGA GTAAAGCTTT CCATGCTGTC
    ATCTCACAGC ACAACAGGCA GGGCTTGGCC TCCCCACCAG TGAGACAGCC ACCTAAGAAG
    GAACCCTCCT TCCACCTGGA CACACCCTCA AAGTTCCTGT CCAAGGACAA GCTTTTCAAG
    CCATCCTTTG ATGTGACGGA TGCACTTGCA GCCTTAGAAA CCCCAAATGC TGCCAGCCAG
    AAGCGGAAAC TGAGCTCACC ACTCTCTGAA GTCATCGTCA GAAACCTGAC AACTGCTTTG
    GCAAATAGTT CACGAAACAC CGGCTGTCTT TCTGCTAGCC CTCAGCTAAA GGGTGCCCAT
    TTGGAGAAGG AAGAGACTCC GAAACCGCTT GACAAAGTTG TGGTGTGCGT GAGTAAAAGG
    CTCAGTAAGA AGCAGAGTGA GCTAAATGGG GTTGCAGCCT CACTGGGAGC AGAGTACAGG
    TGGAGTTTTG ATGAGACAGT TACTCATTAC ATTTACCAGG GTCGGGCAAA TGATAGTAAT
    CGCGAGTATA AATCTGCAAA GGAAAGAGGG GTGCACGTCG TGTCGGAGCA TTGGCTGTTA
    GAGTGTGCTC AAGAGTATAA GCATCTTCCA GAATCTCTTT ACCCACACAC TTACAACCCC
    AAAATGAGCT TGGACATCAG CACAGTGCAG GACGGGCGGC TCTGTGACAG CCGCTTACCC
    TCGGCACTTT CTGCATCTAA GGACGATGAG CCAGATCACC TGTCTGTAGA AGAAAATGCA
    ACAGAGACTT TGGGCACACA CAGCAGAGAG TCCGCTCTGC TGAATGGGAA TGGAAGGGAC
    GACTGCAAAG GAGCTCTGAC CCAGGCCTTG GAGATGAGAG AGAACTTCCA GAAACAACTA
    CAAGAGATCA TGTCAGCAAC CTCCATCGTG AGGACACCAG CCCAGAAGAC TTGCATGTCA
    AGAAGTAGTT GTAACAGCGC CTCTTCAACT CCTGACAGCA CTCGCTCTGC TCGGAGTGGA
    CGAAGCCGAG TCTTGGAGGC GCTCAGGCAG TCTCGACAGA TAGTGCCTGA TATCAACACA
    GAGCCCTCCC AGAATGAACA GATCATTTGG GATGACCCAA CAGCAAGGGA GGAGAGAGCA
    AGGCTTGCCA GCAACCTACA GTGGCCTAGC TACCCAACAC AGTATTCCGA GCTTCAGGTT
    GAGATAAAAA AACTGGATGA TTCTCCTTCT CGAAAGCCTG TGTACCATTC AGAAATTGCT
    GGGCAGGCCT CGTGTGTGAC TCAAGCCCCA GGGCAGCACC CAGGCTCTGA AGAACCAGAA
    GCTCCAGCAG CAGAAAGGAA TCTGATCCCT GAGCCTCAAG CCCCCAGCGT GGCTTTCCCC
    CTGGCTAAGC CGCCTGTAGC TCCACAACCT GAGGACAAGA TTGAAACACT GGAGGAGACG
    CATGGAAAAC TTAAAAAACA GTATGTGTTC CAGATGTCAT CTCTGAATTC TCAAGAGCGG
    ATTGATTACT GTCGCCTGAT TAAAGACCTA GGCCCGGGAG TGCCCAGTGT GATAGTGCAC
    ACATGGAGCG CAGAACAGGT TCCGGGGAGG ACTCCCACCT GTCTTGGCCA AAATAAAAAG
    ATCCATGGAC TCTGCAGTCA GAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa47492
    Clone ID Related Accession (Same CDS sequence) XM_008757856.2 , XM_006243684.2 , XM_008766589.2
    Accession Version XM_008766589.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3987bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product DNA topoisomerase 2-binding protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008766589.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCCAGAA ATGACCAAGA GCCGTTTTTT GTGAAGTTTT TAAAGTCTTC GGACAATTCA 
    GAATGTTTTT TTAAAGCTCT TGAGTCAATA AAGGAGTTAC AATCAGAAGA GTATCTTCAG
    GTTATTACCG ATGAGGAAGC ATTGAGGATT AAAGAGAATG ACAAATCGCT TTATATCTGT
    GACCGTTTCA GTGGCACTGC TTTTGATCAC CTTAAACAGC TTGGCTGCAG AATTGTTGGT
    CCTCAAGTAG TCACATTTTG TATGCGCCAC CAGCAGTGTG TCCCAAGAGC TGAACATCCA
    GTTTATAATA TGATTATGTC TGACATAACT ATATCTTGTA CAAGCCTGGA TAAAGACAAG
    AGGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT
    GTCTCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAATACTT AGTTGCTGCT
    AATCTGAAGA AACCTATTTT GCTTCCTGCT TGGATTAAAA CACTTTGGGA GAAGTCACAA
    GAAAAAAAAA TAACCAAATA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTC
    CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGTATAC ACAGGAAGAC AGTCCAGCAG
    CTCACAGCTA AGCATGGAGG CCAGTACATG GGGCAGCTGA AGATGAACGA GTGCACACAC
    CTCATTGTGC AAGAACCAAA AGGACAAAAA TATGAATGTG CCAGGAGATG GAATGTGCAC
    TGTGTGACCC TGCAATGGTT CCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAGTCT
    ATATACAAGG CCGAACCCAG AGCAGCGGTA AAGACGGTGC CTGATACTTC CACACCCACT
    GCTCAGAGCG GCAGTGCTGC AGCTGAGAAT CACAGGCTGG CAGATGTCAG CCATATATCC
    AACATCAACG GCAGCTGTGT AAATGAAACA ATGTTTGGTT CAACTACGAG CAAACTCGAA
    TGCACACTTG AGAACCTAGA AAATCTGGAC ATCAGTATGT TTCAAGCACC TGAAGATCTA
    TTAGATGGCT GTCGGATATA TCTTTGTGGC TTTAGTGGCA GAAAGCTCGA TAAGTTGAGA
    AGGCTTATTA ACAGTGGAGG TGGAGTTCGG TTTAATCAAC TCAATGAAGA TGTAACTCAT
    GTAATCATGG GAGATTATGA GGATGACGTG AGGCAGTTTT GGAGCAAATC CTCCCACAGG
    CCCCATGTAG TGGGAGCAAA GTGGTTGCTG GAGTGTTTTA GTAAAGGATA TATACTTCCT
    GAAGAGTCAT ATATCCACGC TAACTACCAG CCAGATGGAA TTCCAGTTTC AGATCAGCCC
    AGAAACCAGA CAACTGTCTT AGAAAAGAGT GGCACATTCT CTAAGAAGGA CTTTGTTCCT
    AATGAGAAGT TTCAGCAGAC TGATGAGGAT CTGCTTGCTC AGTATGTACA TGATGACTCC
    ACAATAGTTG AAGCTAAGCT ATCTGAAGCT ATAGAGCCTG AAGTTGGGCC TTGCGGTGGT
    TCTGCTCATG TTGAGCCCTG TGATGATTCC ACTCACATTT CTGTGCAAGA GGAAAACAAG
    TCGTCTGTCA GTCATTGCCT CCTTGATGGC TCTACAGTTC CTGAGGAAGG CTTATTTAGC
    CAAAAGAGTT TCCTTGTTTT GGGTTTTAGT GTTGAAAATG AATGTAATAT TGTAAACATC
    ATAAGAGAGC ATGCTGGAAA GATTGTGTCC CTTCCCAGCA GAATTGTTGC TGACTATGCC
    GTGGTTCCCC TGCTGGGATG TGAAGTAGAA GCGACAGTGG CAGAAGTTGT TACAAACACA
    TGGCTGGTTA CATGCATAGA CAACCAGACA TTAGTTGATC CCAAGTCGAA TCCCCTCTTC
    ACGCCAGTCT CAGTAATGTC AGGAATGACT CCTTTAGAGG ACTGTGTGAT TTCCTTCAGT
    CAGTGTGTGG GAGCAGAAAG AGATTCCTTG GTATTCTTAG CAAATCATCT TGGAGCAAGT
    GTTCAAGAAT TCTTTGTCCG CAAATCCAAT GCAAAGAAAG GCATGCTCGC CAGTACACAT
    CTTATAGTGA AAGAACCGAC TGGTTCTAAA TATGAAGCTG CAAAGAAATG GAGTTTGCCA
    GCAGTTAATA TTTCGTGGCT CTTAGAAACT GCTAGAATGG GGAAGAGAGC AGATGAAAAC
    CATTTTCTGG TTGACAGTGC ACCTAAACAA GAACAAGTAT TAGAAACTAA CATACCAAAT
    GGAGTGAGTC CAAATCCAGA TTCTCCAGCA CATCCTGATG CACACTTGGA AATTCACAGA
    CAAAAGGCCG TTACACCTTT GGACATGAAC CGATTTCAGA GTAAAGCTTT CCATGCTGTC
    ATCTCACAGC ACAACAGGCA GGGCTTGGCC TCCCCACCAG TGAGACAGCC ACCTAAGAAG
    GAACCCTCCT TCCACCTGGA CACACCCTCA AAGTTCCTGT CCAAGGACAA GCTTTTCAAG
    CCATCCTTTG ATGTGACGGA TGCACTTGCA GCCTTAGAAA CCCCAAATGC TGCCAGCCAG
    AAGCGGAAAC TGAGCTCACC ACTCTCTGAA GTCATCGTCA GAAACCTGAC AACTGCTTTG
    GCAAATAGTT CACGAAACAC CGGCTGTCTT TCTGCTAGCC CTCAGCTAAA GGGTGCCCAT
    TTGGAGAAGG AAGAGACTCC GAAACCGCTT GACAAAGTTG TGGTGTGCGT GAGTAAAAGG
    CTCAGTAAGA AGCAGAGTGA GCTAAATGGG GTTGCAGCCT CACTGGGAGC AGAGTACAGG
    TGGAGTTTTG ATGAGACAGT TACTCATTAC ATTTACCAGG GTCGGGCAAA TGATAGTAAT
    CGCGAGTATA AATCTGCAAA GGAAAGAGGG GTGCACGTCG TGTCGGAGCA TTGGCTGTTA
    GAGTGTGCTC AAGAGTATAA GCATCTTCCA GAATCTCTTT ACCCACACAC TTACAACCCC
    AAAATGAGCT TGGACATCAG CACAGTGCAG GACGGGCGGC TCTGTGACAG CCGCTTACCC
    TCGGCACTTT CTGCATCTAA GGACGATGAG CCAGATCACC TGTCTGTAGA AGAAAATGCA
    ACAGAGACTT TGGGCACACA CAGCAGAGAG TCCGCTCTGC TGAATGGGAA TGGAAGGGAC
    GACTGCAAAG GAGCTCTGAC CCAGGCCTTG GAGATGAGAG AGAACTTCCA GAAACAACTA
    CAAGAGATCA TGTCAGCAAC CTCCATCGTG AGGACACCAG CCCAGAAGAC TTGCATGTCA
    AGAAGTAGTT GTAACAGCGC CTCTTCAACT CCTGACAGCA CTCGCTCTGC TCGGAGTGGA
    CGAAGCCGAG TCTTGGAGGC GCTCAGGCAG TCTCGACAGA TAGTGCCTGA TATCAACACA
    GAGCCCTCCC AGAATGAACA GATCATTTGG GATGACCCAA CAGCAAGGGA GGAGAGAGCA
    AGGCTTGCCA GCAACCTACA GTGGCCTAGC TACCCAACAC AGTATTCCGA GCTTCAGGTT
    GAGATAAAAA AACTGGATGA TTCTCCTTCT CGAAAGCCTG TGTACCATTC AGAAATTGCT
    GGGCAGGCCT CGTGTGTGAC TCAAGCCCCA GGGCAGCACC CAGGCTCTGA AGAACCAGAA
    GCTCCAGCAG CAGAAAGGAA TCTGATCCCT GAGCCTCAAG CCCCCAGCGT GGCTTTCCCC
    CTGGCTAAGC CGCCTGTAGC TCCACAACCT GAGGACAAGA TTGAAACACT GGAGGAGACG
    CATGGAAAAC TTAAAAAACA GTATGTGTTC CAGATGTCAT CTCTGAATTC TCAAGAGCGG
    ATTGATTACT GTCGCCTGAT TAAAGACCTA GGCCCGGGAG TGCCCAGTGT GATAGTGCAC
    ACATGGAGCG CAGAACAGGT TCCGGGGAGG ACTCCCACCT GTCTTGGCCA AAATAAAAAG
    ATCCATGGAC TCTGCAGTCA GAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008764811.1
    CDS71..4057
    Translation

    Target ORF information:

    RefSeq Version XM_008766589.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008766589.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCCAGAA ATGACCAAGA GCCGTTTTTT GTGAAGTTTT TAAAGTCTTC GGACAATTCA 
    GAATGTTTTT TTAAAGCTCT TGAGTCAATA AAGGAGTTAC AATCAGAAGA GTATCTTCAG
    GTTATTACCG ATGAGGAAGC ATTGAGGATT AAAGAGAATG ACAAATCGCT TTATATCTGT
    GACCGTTTCA GTGGCACTGC TTTTGATCAC CTTAAACAGC TTGGCTGCAG AATTGTTGGT
    CCTCAAGTAG TCACATTTTG TATGCGCCAC CAGCAGTGTG TCCCAAGAGC TGAACATCCA
    GTTTATAATA TGATTATGTC TGACATAACT ATATCTTGTA CAAGCCTGGA TAAAGACAAG
    AGGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT
    GTCTCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAATACTT AGTTGCTGCT
    AATCTGAAGA AACCTATTTT GCTTCCTGCT TGGATTAAAA CACTTTGGGA GAAGTCACAA
    GAAAAAAAAA TAACCAAATA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTC
    CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGTATAC ACAGGAAGAC AGTCCAGCAG
    CTCACAGCTA AGCATGGAGG CCAGTACATG GGGCAGCTGA AGATGAACGA GTGCACACAC
    CTCATTGTGC AAGAACCAAA AGGACAAAAA TATGAATGTG CCAGGAGATG GAATGTGCAC
    TGTGTGACCC TGCAATGGTT CCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAGTCT
    ATATACAAGG CCGAACCCAG AGCAGCGGTA AAGACGGTGC CTGATACTTC CACACCCACT
    GCTCAGAGCG GCAGTGCTGC AGCTGAGAAT CACAGGCTGG CAGATGTCAG CCATATATCC
    AACATCAACG GCAGCTGTGT AAATGAAACA ATGTTTGGTT CAACTACGAG CAAACTCGAA
    TGCACACTTG AGAACCTAGA AAATCTGGAC ATCAGTATGT TTCAAGCACC TGAAGATCTA
    TTAGATGGCT GTCGGATATA TCTTTGTGGC TTTAGTGGCA GAAAGCTCGA TAAGTTGAGA
    AGGCTTATTA ACAGTGGAGG TGGAGTTCGG TTTAATCAAC TCAATGAAGA TGTAACTCAT
    GTAATCATGG GAGATTATGA GGATGACGTG AGGCAGTTTT GGAGCAAATC CTCCCACAGG
    CCCCATGTAG TGGGAGCAAA GTGGTTGCTG GAGTGTTTTA GTAAAGGATA TATACTTCCT
    GAAGAGTCAT ATATCCACGC TAACTACCAG CCAGATGGAA TTCCAGTTTC AGATCAGCCC
    AGAAACCAGA CAACTGTCTT AGAAAAGAGT GGCACATTCT CTAAGAAGGA CTTTGTTCCT
    AATGAGAAGT TTCAGCAGAC TGATGAGGAT CTGCTTGCTC AGTATGTACA TGATGACTCC
    ACAATAGTTG AAGCTAAGCT ATCTGAAGCT ATAGAGCCTG AAGTTGGGCC TTGCGGTGGT
    TCTGCTCATG TTGAGCCCTG TGATGATTCC ACTCACATTT CTGTGCAAGA GGAAAACAAG
    TCGTCTGTCA GTCATTGCCT CCTTGATGGC TCTACAGTTC CTGAGGAAGG CTTATTTAGC
    CAAAAGAGTT TCCTTGTTTT GGGTTTTAGT GTTGAAAATG AATGTAATAT TGTAAACATC
    ATAAGAGAGC ATGCTGGAAA GATTGTGTCC CTTCCCAGCA GAATTGTTGC TGACTATGCC
    GTGGTTCCCC TGCTGGGATG TGAAGTAGAA GCGACAGTGG CAGAAGTTGT TACAAACACA
    TGGCTGGTTA CATGCATAGA CAACCAGACA TTAGTTGATC CCAAGTCGAA TCCCCTCTTC
    ACGCCAGTCT CAGTAATGTC AGGAATGACT CCTTTAGAGG ACTGTGTGAT TTCCTTCAGT
    CAGTGTGTGG GAGCAGAAAG AGATTCCTTG GTATTCTTAG CAAATCATCT TGGAGCAAGT
    GTTCAAGAAT TCTTTGTCCG CAAATCCAAT GCAAAGAAAG GCATGCTCGC CAGTACACAT
    CTTATAGTGA AAGAACCGAC TGGTTCTAAA TATGAAGCTG CAAAGAAATG GAGTTTGCCA
    GCAGTTAATA TTTCGTGGCT CTTAGAAACT GCTAGAATGG GGAAGAGAGC AGATGAAAAC
    CATTTTCTGG TTGACAGTGC ACCTAAACAA GAACAAGTAT TAGAAACTAA CATACCAAAT
    GGAGTGAGTC CAAATCCAGA TTCTCCAGCA CATCCTGATG CACACTTGGA AATTCACAGA
    CAAAAGGCCG TTACACCTTT GGACATGAAC CGATTTCAGA GTAAAGCTTT CCATGCTGTC
    ATCTCACAGC ACAACAGGCA GGGCTTGGCC TCCCCACCAG TGAGACAGCC ACCTAAGAAG
    GAACCCTCCT TCCACCTGGA CACACCCTCA AAGTTCCTGT CCAAGGACAA GCTTTTCAAG
    CCATCCTTTG ATGTGACGGA TGCACTTGCA GCCTTAGAAA CCCCAAATGC TGCCAGCCAG
    AAGCGGAAAC TGAGCTCACC ACTCTCTGAA GTCATCGTCA GAAACCTGAC AACTGCTTTG
    GCAAATAGTT CACGAAACAC CGGCTGTCTT TCTGCTAGCC CTCAGCTAAA GGGTGCCCAT
    TTGGAGAAGG AAGAGACTCC GAAACCGCTT GACAAAGTTG TGGTGTGCGT GAGTAAAAGG
    CTCAGTAAGA AGCAGAGTGA GCTAAATGGG GTTGCAGCCT CACTGGGAGC AGAGTACAGG
    TGGAGTTTTG ATGAGACAGT TACTCATTAC ATTTACCAGG GTCGGGCAAA TGATAGTAAT
    CGCGAGTATA AATCTGCAAA GGAAAGAGGG GTGCACGTCG TGTCGGAGCA TTGGCTGTTA
    GAGTGTGCTC AAGAGTATAA GCATCTTCCA GAATCTCTTT ACCCACACAC TTACAACCCC
    AAAATGAGCT TGGACATCAG CACAGTGCAG GACGGGCGGC TCTGTGACAG CCGCTTACCC
    TCGGCACTTT CTGCATCTAA GGACGATGAG CCAGATCACC TGTCTGTAGA AGAAAATGCA
    ACAGAGACTT TGGGCACACA CAGCAGAGAG TCCGCTCTGC TGAATGGGAA TGGAAGGGAC
    GACTGCAAAG GAGCTCTGAC CCAGGCCTTG GAGATGAGAG AGAACTTCCA GAAACAACTA
    CAAGAGATCA TGTCAGCAAC CTCCATCGTG AGGACACCAG CCCAGAAGAC TTGCATGTCA
    AGAAGTAGTT GTAACAGCGC CTCTTCAACT CCTGACAGCA CTCGCTCTGC TCGGAGTGGA
    CGAAGCCGAG TCTTGGAGGC GCTCAGGCAG TCTCGACAGA TAGTGCCTGA TATCAACACA
    GAGCCCTCCC AGAATGAACA GATCATTTGG GATGACCCAA CAGCAAGGGA GGAGAGAGCA
    AGGCTTGCCA GCAACCTACA GTGGCCTAGC TACCCAACAC AGTATTCCGA GCTTCAGGTT
    GAGATAAAAA AACTGGATGA TTCTCCTTCT CGAAAGCCTG TGTACCATTC AGAAATTGCT
    GGGCAGGCCT CGTGTGTGAC TCAAGCCCCA GGGCAGCACC CAGGCTCTGA AGAACCAGAA
    GCTCCAGCAG CAGAAAGGAA TCTGATCCCT GAGCCTCAAG CCCCCAGCGT GGCTTTCCCC
    CTGGCTAAGC CGCCTGTAGC TCCACAACCT GAGGACAAGA TTGAAACACT GGAGGAGACG
    CATGGAAAAC TTAAAAAACA GTATGTGTTC CAGATGTCAT CTCTGAATTC TCAAGAGCGG
    ATTGATTACT GTCGCCTGAT TAAAGACCTA GGCCCGGGAG TGCCCAGTGT GATAGTGCAC
    ACATGGAGCG CAGAACAGGT TCCGGGGAGG ACTCCCACCT GTCTTGGCCA AAATAAAAAG
    ATCCATGGAC TCTGCAGTCA GAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa18094
    Clone ID Related Accession (Same CDS sequence) XM_006226542.2
    Accession Version XM_006226542.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4200bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product DNA topoisomerase 2-binding protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000076.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006226542.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT 
    GTCTCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAATACTT AGTTGCTGCT
    AATCTGAAGA AACCTATTTT GCTTCCTGCT TGGATTAAAA CACTTTGGGA GAAGTCACAA
    GAAAAAAAAA TAACCAAATA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTC
    CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGTATAC ACAGGAAGAC AGTCCAGCAG
    CTCACAGCTA AGCATGGAGG CCAGTACATG GGGCAGCTGA AGATGAACGA GTGCACACAC
    CTCATTGTGC AAGAACCAAA AGGACAAAAA TATGAATGTG CCAGGAGATG GAATGTGCAC
    TGTGTGACCC TGCAATGGTT CCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAGTCT
    ATATACAAGG CCGAACCCAG AGCAGCGGTA AAGACGGTGC CTGATACTTC CACACCCACT
    GCTCAGAGCG GCAGTGCTGC AGCTGAGAAT CACAGGCTGG CAGATGTCAG CCATATATCC
    AACATCAACG GCAGCTGTGT AAATGAAACA ATGTTTGGTT CAACTACGAG CAAACTCGAA
    TGCACACTTG AGAACCTAGA AAATCTGGAC ATCAGTATGT TTCAAGCACC TGAAGATCTA
    TTAGATGGCT GTCGGATATA TCTTTGTGGC TTTAGTGGCA GAAAGCTCGA TAAGTTGAGA
    AGGCTTATTA ACAGTGGAGG TGGAGTTCGG TTTAATCAAC TCAATGAAGA TGTAACTCAT
    GTAATCATGG GAGATTATGA GGATGACGTG AGGCAGTTTT GGAGCAAATC CTCCCACAGG
    CCCCATGTAG TGGGAGCAAA GTGGTTGCTG GAGTGTTTTA GTAAAGGATA TATACTTCCT
    GAAGAGTCAT ATATCCACGC TAACTACCAG CCAGATGGAA TTCCAGTTTC AGATCAGCCC
    AGAAACCAGA CAACTGTCTT AGAAAAGAGT GGCACATTCT CTAAGAAGGA CTTTGTTCCT
    AATGAGAAGT TTCAGCAGAC TGATGAGGAT CTGCTTGCTC AGTATGTACA TGATGACTCC
    ACAATAGTTG AAGCTAAGCT ATCTGAAGCT ATAGAGCCTG AAGTTGGGCC TTGCGGTGGT
    TCTGCTCATG TTGAGCCCTG TGATGATTCC ACTCACATTT CTGTGCAAGA GGAAAACAAG
    TCGTCTGTCA GTCATTGCCT CCTTGATGGC TCTACAGTTC CTGAGGAAGG CTTATTTAGC
    CAAAAGAGTT TCCTTGTTTT GGGTTTTAGT GTTGAAAATG AATGTAATAT TGTAAACATC
    ATAAGAGAGC ATGCTGGAAA GATTGTGTCC CTTCCCAGCA GAATTGTTGC TGACTATGCC
    GTGGTTCCCC TGCTGGGATG TGAAGTAGAA GCGACAGTGG CAGAAGTTGT TACAAACACA
    TGGCTGGTTA CATGCATAGA CAACCAGACA TTAGTTGATC CCAAGTCGAA TCCCCTCTTC
    ACGCCAGTCT CAGTAATGTC AGGAATGACT CCTTTAGAGG ACTGTGTGAT TTCCTTCAGT
    CAGTGTGTGG GAGCAGAAAG AGATTCCTTG GTATTCTTAG CAAATCATCT TGGAGCAAGT
    GTTCAAGAAT TCTTTGTCCG CAAATCCAAT GCAAAGAAAG GCATGCTCGC CAGTACACAT
    CTTATAGTGA AAGAACCGAC TGGTTCTAAA TATGAAGCTG CAAAGAAATG GAGTTTGCCA
    GCAGTTAATA TTTCGTGGCT CTTAGAAACT GCTAGAATGG GGAAGAGAGC AGATGAAAAC
    CATTTTCTGG TTGACAGTGC ACCTAAACAA GAACAAGTAT TAGAAACTAA CATACCAAAT
    GGAGTGAGTC CAAATCCAGA TTCTCCAGCA CATCCTGATG CACACTTGGA AATTCACAGA
    CAAAAGGCCG TTACACCTTT GGACATGAAC CGATTTCAGA GTAAAGCTTT CCATGCTGTC
    ATCTCACAGC ACAACAGGCA GGGCTTGGCC TCCCCACCAG TGAGACAGCC ACCTAAGAAG
    GAACCCTCCT TCCACCTGGA CACACCCTCA AAGTTCCTGT CCAAGGACAA GCTTTTCAAG
    CCATCCTTTG ATGTGACGGA TGCACTTGCA GCCTTAGAAA CCCCAAATGC TGCCAGCCAG
    AAGCGGAAAC TGAGCTCACC ACTCTCTGAA GTCATCGTCA GAAACCTGAC AACTGCTTTG
    GCAAATAGTT CACGAAACAC CGGCTGTCTT TCTGCTAGCC CTCAGCTAAA GGGTGCCCAT
    TTGGAGAAGG AAGAGACTCC GAAACCGCTT GACAAAGTTG TGGTGTGCGT GAGTAAAAGG
    CTCAGTAAGA AGCAGAGTGA GCTAAATGGG GTTGCAGCCT CACTGGGAGC AGAGTACAGG
    TGGAGTTTTG ATGAGACAGT TACTCATTAC ATTTACCAGG GTCGGGCAAA TGATAGTAAT
    CGCGAGTATA AATCTGCAAA GGAAAGAGGG GTGCACGTCG TGTCGGAGCA TTGGCTGTTA
    GAGTGTGCTC AAGAGTATAA GCATCTTCCA GAATCTCTTT ACCCACACAC TTACAACCCC
    AAAATGAGCT TGGACATCAG CACAGTGCAG GACGGGCGGC TCTGTGACAG CCGCTTACCC
    TCGGCACTTT CTGCATCTAA GGACGATGAG CCAGATCACC TGTCTGTAGA AGAAAATGCA
    ACAGAGACTT TGGGCACACA CAGCAGAGAG TCCGCTCTGC TGAATGGGAA TGGAAGGGAC
    GACTGCAAAG GAGCTCTGAC CCAGGCCTTG GAGATGAGAG AGAACTTCCA GAAACAACTA
    CAAGAGATCA TGTCAGCAAC CTCCATCGTG AGGACACCAG CCCAGAAGAC TTGCATGTCA
    AGAAGTAGTT GTAACAGCGC CTCTTCAACT CCTGACAGCA CTCGCTCTGC TCGGAGTGGA
    CGAAGCCGAG TCTTGGAGGC GCTCAGGCAG TCTCGACAGA TAGTGCCTGA TATCAACACA
    GAGCCCTCCC AGAATGAACA GATCATTTGG GATGACCCAA CAGCAAGGGA GGAGAGAGCA
    AGGCTTGCCA GCAACCTACA GTGGCCTAGC TACCCAACAC AGTATTCCGA GCTTCAGGTT
    GAGATAAAAA AACTGGATGA TTCTCCTTCT CGAAAGCCTG TGTACCATTC AGAAATTGCT
    GGGCAGGCCT CGTGTGTGAC TCAAGCCCCA GGGCAGCACC CAGGCTCTGA AGAACCAGAA
    GCTCCAGCAG CAGAAAGGAA TCTGATCCCT GAGCCTCAAG CCCCCAGCGT GGCTTTCCCC
    CTGGCTAAGC CGCCTGTAGC TCCACAACCT GAGGACAAGA TTGAAACACT GGAGGAGACG
    CATGGAAAAC TTAAAAAACA GTATGTGTTC CAGATGTCAT CTCTGAATTC TCAAGAGCGG
    ATTGATTACT GTCGCCTGAT TAAAGACCTA GGTGGGTCGG TGATCGAGAA GCAGTGCTCA
    GACCCCAGCT GCACACACAT GGTTGTGGGG TACCCACTGC GCAATGAGAA ATACTTAGCT
    TCCATGGCAG CTGGCAAATG GGTGCTTCAT CGCTCCTACC TGGACGCTTG CCGGACAGCT
    GGGCGCTTTG TGCAGGAGGA AGACTACGAG TGGGGAAGCA GCTCCATCCT AGATGCTCTC
    CCTGACGTCA CTGAGCACCA GCAGAAGCTC GCACTTGCAG CAATGAGATG GAGGAAAAGA
    ATCCAGCAGA GTCGGGAGTC GGGCATCGCT GAGGGAGCAT TTAGTGGCTG GAAAGTTATT
    TTGCGCGTTG ATCGGCCCCG AGAAGCAGGG TTTAAGCGCC TTCTTCAGGC AGGAGGAGCA
    AAGGTATTGC CTGGTCATCC AGAGCCTTTA TTTAAGGATG CTACACATCT CTTCTGTGAT
    TTCAATAAAC TGAAGCCTGA TGACTGCAGA GTTTTTATCG CAGAGGCTAC GGCCCAGAAC
    ATGGTCTGCT TGAAAACAGA ATATATTGCT GATTACCTCA TGATGGAATC CCCTCCTTGT
    GCTGATAATT ACCGAGTATC CGAAACTGCT TTATTCCGTA ACAAGGAAGG TGGGCCTGGA
    CTACCACAGA AGAGGAAAAC TCCTGCAGAG AACGTCATCA AACGGCCTCG GGTGCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006226604.1
    CDS124..4323
    Translation

    Target ORF information:

    RefSeq Version XM_006226542.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006226542.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT 
    GTCTCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAATACTT AGTTGCTGCT
    AATCTGAAGA AACCTATTTT GCTTCCTGCT TGGATTAAAA CACTTTGGGA GAAGTCACAA
    GAAAAAAAAA TAACCAAATA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTC
    CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGTATAC ACAGGAAGAC AGTCCAGCAG
    CTCACAGCTA AGCATGGAGG CCAGTACATG GGGCAGCTGA AGATGAACGA GTGCACACAC
    CTCATTGTGC AAGAACCAAA AGGACAAAAA TATGAATGTG CCAGGAGATG GAATGTGCAC
    TGTGTGACCC TGCAATGGTT CCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAGTCT
    ATATACAAGG CCGAACCCAG AGCAGCGGTA AAGACGGTGC CTGATACTTC CACACCCACT
    GCTCAGAGCG GCAGTGCTGC AGCTGAGAAT CACAGGCTGG CAGATGTCAG CCATATATCC
    AACATCAACG GCAGCTGTGT AAATGAAACA ATGTTTGGTT CAACTACGAG CAAACTCGAA
    TGCACACTTG AGAACCTAGA AAATCTGGAC ATCAGTATGT TTCAAGCACC TGAAGATCTA
    TTAGATGGCT GTCGGATATA TCTTTGTGGC TTTAGTGGCA GAAAGCTCGA TAAGTTGAGA
    AGGCTTATTA ACAGTGGAGG TGGAGTTCGG TTTAATCAAC TCAATGAAGA TGTAACTCAT
    GTAATCATGG GAGATTATGA GGATGACGTG AGGCAGTTTT GGAGCAAATC CTCCCACAGG
    CCCCATGTAG TGGGAGCAAA GTGGTTGCTG GAGTGTTTTA GTAAAGGATA TATACTTCCT
    GAAGAGTCAT ATATCCACGC TAACTACCAG CCAGATGGAA TTCCAGTTTC AGATCAGCCC
    AGAAACCAGA CAACTGTCTT AGAAAAGAGT GGCACATTCT CTAAGAAGGA CTTTGTTCCT
    AATGAGAAGT TTCAGCAGAC TGATGAGGAT CTGCTTGCTC AGTATGTACA TGATGACTCC
    ACAATAGTTG AAGCTAAGCT ATCTGAAGCT ATAGAGCCTG AAGTTGGGCC TTGCGGTGGT
    TCTGCTCATG TTGAGCCCTG TGATGATTCC ACTCACATTT CTGTGCAAGA GGAAAACAAG
    TCGTCTGTCA GTCATTGCCT CCTTGATGGC TCTACAGTTC CTGAGGAAGG CTTATTTAGC
    CAAAAGAGTT TCCTTGTTTT GGGTTTTAGT GTTGAAAATG AATGTAATAT TGTAAACATC
    ATAAGAGAGC ATGCTGGAAA GATTGTGTCC CTTCCCAGCA GAATTGTTGC TGACTATGCC
    GTGGTTCCCC TGCTGGGATG TGAAGTAGAA GCGACAGTGG CAGAAGTTGT TACAAACACA
    TGGCTGGTTA CATGCATAGA CAACCAGACA TTAGTTGATC CCAAGTCGAA TCCCCTCTTC
    ACGCCAGTCT CAGTAATGTC AGGAATGACT CCTTTAGAGG ACTGTGTGAT TTCCTTCAGT
    CAGTGTGTGG GAGCAGAAAG AGATTCCTTG GTATTCTTAG CAAATCATCT TGGAGCAAGT
    GTTCAAGAAT TCTTTGTCCG CAAATCCAAT GCAAAGAAAG GCATGCTCGC CAGTACACAT
    CTTATAGTGA AAGAACCGAC TGGTTCTAAA TATGAAGCTG CAAAGAAATG GAGTTTGCCA
    GCAGTTAATA TTTCGTGGCT CTTAGAAACT GCTAGAATGG GGAAGAGAGC AGATGAAAAC
    CATTTTCTGG TTGACAGTGC ACCTAAACAA GAACAAGTAT TAGAAACTAA CATACCAAAT
    GGAGTGAGTC CAAATCCAGA TTCTCCAGCA CATCCTGATG CACACTTGGA AATTCACAGA
    CAAAAGGCCG TTACACCTTT GGACATGAAC CGATTTCAGA GTAAAGCTTT CCATGCTGTC
    ATCTCACAGC ACAACAGGCA GGGCTTGGCC TCCCCACCAG TGAGACAGCC ACCTAAGAAG
    GAACCCTCCT TCCACCTGGA CACACCCTCA AAGTTCCTGT CCAAGGACAA GCTTTTCAAG
    CCATCCTTTG ATGTGACGGA TGCACTTGCA GCCTTAGAAA CCCCAAATGC TGCCAGCCAG
    AAGCGGAAAC TGAGCTCACC ACTCTCTGAA GTCATCGTCA GAAACCTGAC AACTGCTTTG
    GCAAATAGTT CACGAAACAC CGGCTGTCTT TCTGCTAGCC CTCAGCTAAA GGGTGCCCAT
    TTGGAGAAGG AAGAGACTCC GAAACCGCTT GACAAAGTTG TGGTGTGCGT GAGTAAAAGG
    CTCAGTAAGA AGCAGAGTGA GCTAAATGGG GTTGCAGCCT CACTGGGAGC AGAGTACAGG
    TGGAGTTTTG ATGAGACAGT TACTCATTAC ATTTACCAGG GTCGGGCAAA TGATAGTAAT
    CGCGAGTATA AATCTGCAAA GGAAAGAGGG GTGCACGTCG TGTCGGAGCA TTGGCTGTTA
    GAGTGTGCTC AAGAGTATAA GCATCTTCCA GAATCTCTTT ACCCACACAC TTACAACCCC
    AAAATGAGCT TGGACATCAG CACAGTGCAG GACGGGCGGC TCTGTGACAG CCGCTTACCC
    TCGGCACTTT CTGCATCTAA GGACGATGAG CCAGATCACC TGTCTGTAGA AGAAAATGCA
    ACAGAGACTT TGGGCACACA CAGCAGAGAG TCCGCTCTGC TGAATGGGAA TGGAAGGGAC
    GACTGCAAAG GAGCTCTGAC CCAGGCCTTG GAGATGAGAG AGAACTTCCA GAAACAACTA
    CAAGAGATCA TGTCAGCAAC CTCCATCGTG AGGACACCAG CCCAGAAGAC TTGCATGTCA
    AGAAGTAGTT GTAACAGCGC CTCTTCAACT CCTGACAGCA CTCGCTCTGC TCGGAGTGGA
    CGAAGCCGAG TCTTGGAGGC GCTCAGGCAG TCTCGACAGA TAGTGCCTGA TATCAACACA
    GAGCCCTCCC AGAATGAACA GATCATTTGG GATGACCCAA CAGCAAGGGA GGAGAGAGCA
    AGGCTTGCCA GCAACCTACA GTGGCCTAGC TACCCAACAC AGTATTCCGA GCTTCAGGTT
    GAGATAAAAA AACTGGATGA TTCTCCTTCT CGAAAGCCTG TGTACCATTC AGAAATTGCT
    GGGCAGGCCT CGTGTGTGAC TCAAGCCCCA GGGCAGCACC CAGGCTCTGA AGAACCAGAA
    GCTCCAGCAG CAGAAAGGAA TCTGATCCCT GAGCCTCAAG CCCCCAGCGT GGCTTTCCCC
    CTGGCTAAGC CGCCTGTAGC TCCACAACCT GAGGACAAGA TTGAAACACT GGAGGAGACG
    CATGGAAAAC TTAAAAAACA GTATGTGTTC CAGATGTCAT CTCTGAATTC TCAAGAGCGG
    ATTGATTACT GTCGCCTGAT TAAAGACCTA GGTGGGTCGG TGATCGAGAA GCAGTGCTCA
    GACCCCAGCT GCACACACAT GGTTGTGGGG TACCCACTGC GCAATGAGAA ATACTTAGCT
    TCCATGGCAG CTGGCAAATG GGTGCTTCAT CGCTCCTACC TGGACGCTTG CCGGACAGCT
    GGGCGCTTTG TGCAGGAGGA AGACTACGAG TGGGGAAGCA GCTCCATCCT AGATGCTCTC
    CCTGACGTCA CTGAGCACCA GCAGAAGCTC GCACTTGCAG CAATGAGATG GAGGAAAAGA
    ATCCAGCAGA GTCGGGAGTC GGGCATCGCT GAGGGAGCAT TTAGTGGCTG GAAAGTTATT
    TTGCGCGTTG ATCGGCCCCG AGAAGCAGGG TTTAAGCGCC TTCTTCAGGC AGGAGGAGCA
    AAGGTATTGC CTGGTCATCC AGAGCCTTTA TTTAAGGATG CTACACATCT CTTCTGTGAT
    TTCAATAAAC TGAAGCCTGA TGACTGCAGA GTTTTTATCG CAGAGGCTAC GGCCCAGAAC
    ATGGTCTGCT TGAAAACAGA ATATATTGCT GATTACCTCA TGATGGAATC CCCTCCTTGT
    GCTGATAATT ACCGAGTATC CGAAACTGCT TTATTCCGTA ACAAGGAAGG TGGGCCTGGA
    CTACCACAGA AGAGGAAAAC TCCTGCAGAG AACGTCATCA AACGGCCTCG GGTGCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa47491
    Clone ID Related Accession (Same CDS sequence) XM_236578.8 , XM_001072207.5 , XM_008757855.2
    Accession Version XM_236578.8 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4560bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product DNA topoisomerase 2-binding protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_236578.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCCAGAA ATGACCAAGA GCCGTTTTTT GTGAAGTTTT TAAAGTCTTC GGACAATTCA 
    GAATGTTTTT TTAAAGCTCT TGAGTCAATA AAGGAGTTAC AATCAGAAGA GTATCTTCAG
    GTTATTACCG ATGAGGAAGC ATTGAGGATT AAAGAGAATG ACAAATCGCT TTATATCTGT
    GACCGTTTCA GTGGCACTGC TTTTGATCAC CTTAAACAGC TTGGCTGCAG AATTGTTGGT
    CCTCAAGTAG TCACATTTTG TATGCGCCAC CAGCAGTGTG TCCCAAGAGC TGAACATCCA
    GTTTATAATA TGATTATGTC TGACATAACT ATATCTTGTA CAAGCCTGGA TAAAGACAAG
    AGGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT
    GTCTCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAATACTT AGTTGCTGCT
    AATCTGAAGA AACCTATTTT GCTTCCTGCT TGGATTAAAA CACTTTGGGA GAAGTCACAA
    GAAAAAAAAA TAACCAAATA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTC
    CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGTATAC ACAGGAAGAC AGTCCAGCAG
    CTCACAGCTA AGCATGGAGG CCAGTACATG GGGCAGCTGA AGATGAACGA GTGCACACAC
    CTCATTGTGC AAGAACCAAA AGGACAAAAA TATGAATGTG CCAGGAGATG GAATGTGCAC
    TGTGTGACCC TGCAATGGTT CCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAGTCT
    ATATACAAGG CCGAACCCAG AGCAGCGGTA AAGACGGTGC CTGATACTTC CACACCCACT
    GCTCAGAGCG GCAGTGCTGC AGCTGAGAAT CACAGGCTGG CAGATGTCAG CCATATATCC
    AACATCAACG GCAGCTGTGT AAATGAAACA ATGTTTGGTT CAACTACGAG CAAACTCGAA
    TGCACACTTG AGAACCTAGA AAATCTGGAC ATCAGTATGT TTCAAGCACC TGAAGATCTA
    TTAGATGGCT GTCGGATATA TCTTTGTGGC TTTAGTGGCA GAAAGCTCGA TAAGTTGAGA
    AGGCTTATTA ACAGTGGAGG TGGAGTTCGG TTTAATCAAC TCAATGAAGA TGTAACTCAT
    GTAATCATGG GAGATTATGA GGATGACGTG AGGCAGTTTT GGAGCAAATC CTCCCACAGG
    CCCCATGTAG TGGGAGCAAA GTGGTTGCTG GAGTGTTTTA GTAAAGGATA TATACTTCCT
    GAAGAGTCAT ATATCCACGC TAACTACCAG CCAGATGGAA TTCCAGTTTC AGATCAGCCC
    AGAAACCAGA CAACTGTCTT AGAAAAGAGT GGCACATTCT CTAAGAAGGA CTTTGTTCCT
    AATGAGAAGT TTCAGCAGAC TGATGAGGAT CTGCTTGCTC AGTATGTACA TGATGACTCC
    ACAATAGTTG AAGCTAAGCT ATCTGAAGCT ATAGAGCCTG AAGTTGGGCC TTGCGGTGGT
    TCTGCTCATG TTGAGCCCTG TGATGATTCC ACTCACATTT CTGTGCAAGA GGAAAACAAG
    TCGTCTGTCA GTCATTGCCT CCTTGATGGC TCTACAGTTC CTGAGGAAGG CTTATTTAGC
    CAAAAGAGTT TCCTTGTTTT GGGTTTTAGT GTTGAAAATG AATGTAATAT TGTAAACATC
    ATAAGAGAGC ATGCTGGAAA GATTGTGTCC CTTCCCAGCA GAATTGTTGC TGACTATGCC
    GTGGTTCCCC TGCTGGGATG TGAAGTAGAA GCGACAGTGG CAGAAGTTGT TACAAACACA
    TGGCTGGTTA CATGCATAGA CAACCAGACA TTAGTTGATC CCAAGTCGAA TCCCCTCTTC
    ACGCCAGTCT CAGTAATGTC AGGAATGACT CCTTTAGAGG ACTGTGTGAT TTCCTTCAGT
    CAGTGTGTGG GAGCAGAAAG AGATTCCTTG GTATTCTTAG CAAATCATCT TGGAGCAAGT
    GTTCAAGAAT TCTTTGTCCG CAAATCCAAT GCAAAGAAAG GCATGCTCGC CAGTACACAT
    CTTATAGTGA AAGAACCGAC TGGTTCTAAA TATGAAGCTG CAAAGAAATG GAGTTTGCCA
    GCAGTTAATA TTTCGTGGCT CTTAGAAACT GCTAGAATGG GGAAGAGAGC AGATGAAAAC
    CATTTTCTGG TTGACAGTGC ACCTAAACAA GAACAAGTAT TAGAAACTAA CATACCAAAT
    GGAGTGAGTC CAAATCCAGA TTCTCCAGCA CATCCTGATG CACACTTGGA AATTCACAGA
    CAAAAGGCCG TTACACCTTT GGACATGAAC CGATTTCAGA GTAAAGCTTT CCATGCTGTC
    ATCTCACAGC ACAACAGGCA GGGCTTGGCC TCCCCACCAG TGAGACAGCC ACCTAAGAAG
    GAACCCTCCT TCCACCTGGA CACACCCTCA AAGTTCCTGT CCAAGGACAA GCTTTTCAAG
    CCATCCTTTG ATGTGACGGA TGCACTTGCA GCCTTAGAAA CCCCAAATGC TGCCAGCCAG
    AAGCGGAAAC TGAGCTCACC ACTCTCTGAA GTCATCGTCA GAAACCTGAC AACTGCTTTG
    GCAAATAGTT CACGAAACAC CGGCTGTCTT TCTGCTAGCC CTCAGCTAAA GGGTGCCCAT
    TTGGAGAAGG AAGAGACTCC GAAACCGCTT GACAAAGTTG TGGTGTGCGT GAGTAAAAGG
    CTCAGTAAGA AGCAGAGTGA GCTAAATGGG GTTGCAGCCT CACTGGGAGC AGAGTACAGG
    TGGAGTTTTG ATGAGACAGT TACTCATTAC ATTTACCAGG GTCGGGCAAA TGATAGTAAT
    CGCGAGTATA AATCTGCAAA GGAAAGAGGG GTGCACGTCG TGTCGGAGCA TTGGCTGTTA
    GAGTGTGCTC AAGAGTATAA GCATCTTCCA GAATCTCTTT ACCCACACAC TTACAACCCC
    AAAATGAGCT TGGACATCAG CACAGTGCAG GACGGGCGGC TCTGTGACAG CCGCTTACCC
    TCGGCACTTT CTGCATCTAA GGACGATGAG CCAGATCACC TGTCTGTAGA AGAAAATGCA
    ACAGAGACTT TGGGCACACA CAGCAGAGAG TCCGCTCTGC TGAATGGGAA TGGAAGGGAC
    GACTGCAAAG GAGCTCTGAC CCAGGCCTTG GAGATGAGAG AGAACTTCCA GAAACAACTA
    CAAGAGATCA TGTCAGCAAC CTCCATCGTG AGGACACCAG CCCAGAAGAC TTGCATGTCA
    AGAAGTAGTT GTAACAGCGC CTCTTCAACT CCTGACAGCA CTCGCTCTGC TCGGAGTGGA
    CGAAGCCGAG TCTTGGAGGC GCTCAGGCAG TCTCGACAGA TAGTGCCTGA TATCAACACA
    GAGCCCTCCC AGAATGAACA GATCATTTGG GATGACCCAA CAGCAAGGGA GGAGAGAGCA
    AGGCTTGCCA GCAACCTACA GTGGCCTAGC TACCCAACAC AGTATTCCGA GCTTCAGGTT
    GAGATAAAAA AACTGGATGA TTCTCCTTCT CGAAAGCCTG TGTACCATTC AGAAATTGCT
    GGGCAGGCCT CGTGTGTGAC TCAAGCCCCA GGGCAGCACC CAGGCTCTGA AGAACCAGAA
    GCTCCAGCAG CAGAAAGGAA TCTGATCCCT GAGCCTCAAG CCCCCAGCGT GGCTTTCCCC
    CTGGCTAAGC CGCCTGTAGC TCCACAACCT GAGGACAAGA TTGAAACACT GGAGGAGACG
    CATGGAAAAC TTAAAAAACA GTATGTGTTC CAGATGTCAT CTCTGAATTC TCAAGAGCGG
    ATTGATTACT GTCGCCTGAT TAAAGACCTA GGTGGGTCGG TGATCGAGAA GCAGTGCTCA
    GACCCCAGCT GCACACACAT GGTTGTGGGG TACCCACTGC GCAATGAGAA ATACTTAGCT
    TCCATGGCAG CTGGCAAATG GGTGCTTCAT CGCTCCTACC TGGACGCTTG CCGGACAGCT
    GGGCGCTTTG TGCAGGAGGA AGACTACGAG TGGGGAAGCA GCTCCATCCT AGATGCTCTC
    CCTGACGTCA CTGAGCACCA GCAGAAGCTC GCACTTGCAG CAATGAGATG GAGGAAAAGA
    ATCCAGCAGA GTCGGGAGTC GGGCATCGCT GAGGGAGCAT TTAGTGGCTG GAAAGTTATT
    TTGCGCGTTG ATCGGCCCCG AGAAGCAGGG TTTAAGCGCC TTCTTCAGGC AGGAGGAGCA
    AAGGTATTGC CTGGTCATCC AGAGCCTTTA TTTAAGGATG CTACACATCT CTTCTGTGAT
    TTCAATAAAC TGAAGCCTGA TGACTGCAGA GTTTTTATCG CAGAGGCTAC GGCCCAGAAC
    ATGGTCTGCT TGAAAACAGA ATATATTGCT GATTACCTCA TGATGGAATC CCCTCCTTGT
    GCTGATAATT ACCGAGTATC CGAAACTGCT TTATTCCGTA ACAAGGAAGG TGGGCCTGGA
    CTACCACAGA AGAGGAAAAC TCCTGCAGAG AACGTCATCA AACGGCCTCG GGTGCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_236578.7
    CDS205..4764
    Translation

    Target ORF information:

    RefSeq Version XM_236578.8
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_236578.8

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCCAGAA ATGACCAAGA GCCGTTTTTT GTGAAGTTTT TAAAGTCTTC GGACAATTCA 
    GAATGTTTTT TTAAAGCTCT TGAGTCAATA AAGGAGTTAC AATCAGAAGA GTATCTTCAG
    GTTATTACCG ATGAGGAAGC ATTGAGGATT AAAGAGAATG ACAAATCGCT TTATATCTGT
    GACCGTTTCA GTGGCACTGC TTTTGATCAC CTTAAACAGC TTGGCTGCAG AATTGTTGGT
    CCTCAAGTAG TCACATTTTG TATGCGCCAC CAGCAGTGTG TCCCAAGAGC TGAACATCCA
    GTTTATAATA TGATTATGTC TGACATAACT ATATCTTGTA CAAGCCTGGA TAAAGACAAG
    AGGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT
    GTCTCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAATACTT AGTTGCTGCT
    AATCTGAAGA AACCTATTTT GCTTCCTGCT TGGATTAAAA CACTTTGGGA GAAGTCACAA
    GAAAAAAAAA TAACCAAATA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTC
    CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGTATAC ACAGGAAGAC AGTCCAGCAG
    CTCACAGCTA AGCATGGAGG CCAGTACATG GGGCAGCTGA AGATGAACGA GTGCACACAC
    CTCATTGTGC AAGAACCAAA AGGACAAAAA TATGAATGTG CCAGGAGATG GAATGTGCAC
    TGTGTGACCC TGCAATGGTT CCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAGTCT
    ATATACAAGG CCGAACCCAG AGCAGCGGTA AAGACGGTGC CTGATACTTC CACACCCACT
    GCTCAGAGCG GCAGTGCTGC AGCTGAGAAT CACAGGCTGG CAGATGTCAG CCATATATCC
    AACATCAACG GCAGCTGTGT AAATGAAACA ATGTTTGGTT CAACTACGAG CAAACTCGAA
    TGCACACTTG AGAACCTAGA AAATCTGGAC ATCAGTATGT TTCAAGCACC TGAAGATCTA
    TTAGATGGCT GTCGGATATA TCTTTGTGGC TTTAGTGGCA GAAAGCTCGA TAAGTTGAGA
    AGGCTTATTA ACAGTGGAGG TGGAGTTCGG TTTAATCAAC TCAATGAAGA TGTAACTCAT
    GTAATCATGG GAGATTATGA GGATGACGTG AGGCAGTTTT GGAGCAAATC CTCCCACAGG
    CCCCATGTAG TGGGAGCAAA GTGGTTGCTG GAGTGTTTTA GTAAAGGATA TATACTTCCT
    GAAGAGTCAT ATATCCACGC TAACTACCAG CCAGATGGAA TTCCAGTTTC AGATCAGCCC
    AGAAACCAGA CAACTGTCTT AGAAAAGAGT GGCACATTCT CTAAGAAGGA CTTTGTTCCT
    AATGAGAAGT TTCAGCAGAC TGATGAGGAT CTGCTTGCTC AGTATGTACA TGATGACTCC
    ACAATAGTTG AAGCTAAGCT ATCTGAAGCT ATAGAGCCTG AAGTTGGGCC TTGCGGTGGT
    TCTGCTCATG TTGAGCCCTG TGATGATTCC ACTCACATTT CTGTGCAAGA GGAAAACAAG
    TCGTCTGTCA GTCATTGCCT CCTTGATGGC TCTACAGTTC CTGAGGAAGG CTTATTTAGC
    CAAAAGAGTT TCCTTGTTTT GGGTTTTAGT GTTGAAAATG AATGTAATAT TGTAAACATC
    ATAAGAGAGC ATGCTGGAAA GATTGTGTCC CTTCCCAGCA GAATTGTTGC TGACTATGCC
    GTGGTTCCCC TGCTGGGATG TGAAGTAGAA GCGACAGTGG CAGAAGTTGT TACAAACACA
    TGGCTGGTTA CATGCATAGA CAACCAGACA TTAGTTGATC CCAAGTCGAA TCCCCTCTTC
    ACGCCAGTCT CAGTAATGTC AGGAATGACT CCTTTAGAGG ACTGTGTGAT TTCCTTCAGT
    CAGTGTGTGG GAGCAGAAAG AGATTCCTTG GTATTCTTAG CAAATCATCT TGGAGCAAGT
    GTTCAAGAAT TCTTTGTCCG CAAATCCAAT GCAAAGAAAG GCATGCTCGC CAGTACACAT
    CTTATAGTGA AAGAACCGAC TGGTTCTAAA TATGAAGCTG CAAAGAAATG GAGTTTGCCA
    GCAGTTAATA TTTCGTGGCT CTTAGAAACT GCTAGAATGG GGAAGAGAGC AGATGAAAAC
    CATTTTCTGG TTGACAGTGC ACCTAAACAA GAACAAGTAT TAGAAACTAA CATACCAAAT
    GGAGTGAGTC CAAATCCAGA TTCTCCAGCA CATCCTGATG CACACTTGGA AATTCACAGA
    CAAAAGGCCG TTACACCTTT GGACATGAAC CGATTTCAGA GTAAAGCTTT CCATGCTGTC
    ATCTCACAGC ACAACAGGCA GGGCTTGGCC TCCCCACCAG TGAGACAGCC ACCTAAGAAG
    GAACCCTCCT TCCACCTGGA CACACCCTCA AAGTTCCTGT CCAAGGACAA GCTTTTCAAG
    CCATCCTTTG ATGTGACGGA TGCACTTGCA GCCTTAGAAA CCCCAAATGC TGCCAGCCAG
    AAGCGGAAAC TGAGCTCACC ACTCTCTGAA GTCATCGTCA GAAACCTGAC AACTGCTTTG
    GCAAATAGTT CACGAAACAC CGGCTGTCTT TCTGCTAGCC CTCAGCTAAA GGGTGCCCAT
    TTGGAGAAGG AAGAGACTCC GAAACCGCTT GACAAAGTTG TGGTGTGCGT GAGTAAAAGG
    CTCAGTAAGA AGCAGAGTGA GCTAAATGGG GTTGCAGCCT CACTGGGAGC AGAGTACAGG
    TGGAGTTTTG ATGAGACAGT TACTCATTAC ATTTACCAGG GTCGGGCAAA TGATAGTAAT
    CGCGAGTATA AATCTGCAAA GGAAAGAGGG GTGCACGTCG TGTCGGAGCA TTGGCTGTTA
    GAGTGTGCTC AAGAGTATAA GCATCTTCCA GAATCTCTTT ACCCACACAC TTACAACCCC
    AAAATGAGCT TGGACATCAG CACAGTGCAG GACGGGCGGC TCTGTGACAG CCGCTTACCC
    TCGGCACTTT CTGCATCTAA GGACGATGAG CCAGATCACC TGTCTGTAGA AGAAAATGCA
    ACAGAGACTT TGGGCACACA CAGCAGAGAG TCCGCTCTGC TGAATGGGAA TGGAAGGGAC
    GACTGCAAAG GAGCTCTGAC CCAGGCCTTG GAGATGAGAG AGAACTTCCA GAAACAACTA
    CAAGAGATCA TGTCAGCAAC CTCCATCGTG AGGACACCAG CCCAGAAGAC TTGCATGTCA
    AGAAGTAGTT GTAACAGCGC CTCTTCAACT CCTGACAGCA CTCGCTCTGC TCGGAGTGGA
    CGAAGCCGAG TCTTGGAGGC GCTCAGGCAG TCTCGACAGA TAGTGCCTGA TATCAACACA
    GAGCCCTCCC AGAATGAACA GATCATTTGG GATGACCCAA CAGCAAGGGA GGAGAGAGCA
    AGGCTTGCCA GCAACCTACA GTGGCCTAGC TACCCAACAC AGTATTCCGA GCTTCAGGTT
    GAGATAAAAA AACTGGATGA TTCTCCTTCT CGAAAGCCTG TGTACCATTC AGAAATTGCT
    GGGCAGGCCT CGTGTGTGAC TCAAGCCCCA GGGCAGCACC CAGGCTCTGA AGAACCAGAA
    GCTCCAGCAG CAGAAAGGAA TCTGATCCCT GAGCCTCAAG CCCCCAGCGT GGCTTTCCCC
    CTGGCTAAGC CGCCTGTAGC TCCACAACCT GAGGACAAGA TTGAAACACT GGAGGAGACG
    CATGGAAAAC TTAAAAAACA GTATGTGTTC CAGATGTCAT CTCTGAATTC TCAAGAGCGG
    ATTGATTACT GTCGCCTGAT TAAAGACCTA GGTGGGTCGG TGATCGAGAA GCAGTGCTCA
    GACCCCAGCT GCACACACAT GGTTGTGGGG TACCCACTGC GCAATGAGAA ATACTTAGCT
    TCCATGGCAG CTGGCAAATG GGTGCTTCAT CGCTCCTACC TGGACGCTTG CCGGACAGCT
    GGGCGCTTTG TGCAGGAGGA AGACTACGAG TGGGGAAGCA GCTCCATCCT AGATGCTCTC
    CCTGACGTCA CTGAGCACCA GCAGAAGCTC GCACTTGCAG CAATGAGATG GAGGAAAAGA
    ATCCAGCAGA GTCGGGAGTC GGGCATCGCT GAGGGAGCAT TTAGTGGCTG GAAAGTTATT
    TTGCGCGTTG ATCGGCCCCG AGAAGCAGGG TTTAAGCGCC TTCTTCAGGC AGGAGGAGCA
    AAGGTATTGC CTGGTCATCC AGAGCCTTTA TTTAAGGATG CTACACATCT CTTCTGTGAT
    TTCAATAAAC TGAAGCCTGA TGACTGCAGA GTTTTTATCG CAGAGGCTAC GGCCCAGAAC
    ATGGTCTGCT TGAAAACAGA ATATATTGCT GATTACCTCA TGATGGAATC CCCTCCTTGT
    GCTGATAATT ACCGAGTATC CGAAACTGCT TTATTCCGTA ACAAGGAAGG TGGGCCTGGA
    CTACCACAGA AGAGGAAAAC TCCTGCAGAG AACGTCATCA AACGGCCTCG GGTGCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa47491
    Clone ID Related Accession (Same CDS sequence) XM_236578.8 , XM_001072207.5 , XM_008757855.2
    Accession Version XM_001072207.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4560bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product DNA topoisomerase 2-binding protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000076.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_001072207.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCCAGAA ATGACCAAGA GCCGTTTTTT GTGAAGTTTT TAAAGTCTTC GGACAATTCA 
    GAATGTTTTT TTAAAGCTCT TGAGTCAATA AAGGAGTTAC AATCAGAAGA GTATCTTCAG
    GTTATTACCG ATGAGGAAGC ATTGAGGATT AAAGAGAATG ACAAATCGCT TTATATCTGT
    GACCGTTTCA GTGGCACTGC TTTTGATCAC CTTAAACAGC TTGGCTGCAG AATTGTTGGT
    CCTCAAGTAG TCACATTTTG TATGCGCCAC CAGCAGTGTG TCCCAAGAGC TGAACATCCA
    GTTTATAATA TGATTATGTC TGACATAACT ATATCTTGTA CAAGCCTGGA TAAAGACAAG
    AGGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT
    GTCTCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAATACTT AGTTGCTGCT
    AATCTGAAGA AACCTATTTT GCTTCCTGCT TGGATTAAAA CACTTTGGGA GAAGTCACAA
    GAAAAAAAAA TAACCAAATA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTC
    CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGTATAC ACAGGAAGAC AGTCCAGCAG
    CTCACAGCTA AGCATGGAGG CCAGTACATG GGGCAGCTGA AGATGAACGA GTGCACACAC
    CTCATTGTGC AAGAACCAAA AGGACAAAAA TATGAATGTG CCAGGAGATG GAATGTGCAC
    TGTGTGACCC TGCAATGGTT CCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAGTCT
    ATATACAAGG CCGAACCCAG AGCAGCGGTA AAGACGGTGC CTGATACTTC CACACCCACT
    GCTCAGAGCG GCAGTGCTGC AGCTGAGAAT CACAGGCTGG CAGATGTCAG CCATATATCC
    AACATCAACG GCAGCTGTGT AAATGAAACA ATGTTTGGTT CAACTACGAG CAAACTCGAA
    TGCACACTTG AGAACCTAGA AAATCTGGAC ATCAGTATGT TTCAAGCACC TGAAGATCTA
    TTAGATGGCT GTCGGATATA TCTTTGTGGC TTTAGTGGCA GAAAGCTCGA TAAGTTGAGA
    AGGCTTATTA ACAGTGGAGG TGGAGTTCGG TTTAATCAAC TCAATGAAGA TGTAACTCAT
    GTAATCATGG GAGATTATGA GGATGACGTG AGGCAGTTTT GGAGCAAATC CTCCCACAGG
    CCCCATGTAG TGGGAGCAAA GTGGTTGCTG GAGTGTTTTA GTAAAGGATA TATACTTCCT
    GAAGAGTCAT ATATCCACGC TAACTACCAG CCAGATGGAA TTCCAGTTTC AGATCAGCCC
    AGAAACCAGA CAACTGTCTT AGAAAAGAGT GGCACATTCT CTAAGAAGGA CTTTGTTCCT
    AATGAGAAGT TTCAGCAGAC TGATGAGGAT CTGCTTGCTC AGTATGTACA TGATGACTCC
    ACAATAGTTG AAGCTAAGCT ATCTGAAGCT ATAGAGCCTG AAGTTGGGCC TTGCGGTGGT
    TCTGCTCATG TTGAGCCCTG TGATGATTCC ACTCACATTT CTGTGCAAGA GGAAAACAAG
    TCGTCTGTCA GTCATTGCCT CCTTGATGGC TCTACAGTTC CTGAGGAAGG CTTATTTAGC
    CAAAAGAGTT TCCTTGTTTT GGGTTTTAGT GTTGAAAATG AATGTAATAT TGTAAACATC
    ATAAGAGAGC ATGCTGGAAA GATTGTGTCC CTTCCCAGCA GAATTGTTGC TGACTATGCC
    GTGGTTCCCC TGCTGGGATG TGAAGTAGAA GCGACAGTGG CAGAAGTTGT TACAAACACA
    TGGCTGGTTA CATGCATAGA CAACCAGACA TTAGTTGATC CCAAGTCGAA TCCCCTCTTC
    ACGCCAGTCT CAGTAATGTC AGGAATGACT CCTTTAGAGG ACTGTGTGAT TTCCTTCAGT
    CAGTGTGTGG GAGCAGAAAG AGATTCCTTG GTATTCTTAG CAAATCATCT TGGAGCAAGT
    GTTCAAGAAT TCTTTGTCCG CAAATCCAAT GCAAAGAAAG GCATGCTCGC CAGTACACAT
    CTTATAGTGA AAGAACCGAC TGGTTCTAAA TATGAAGCTG CAAAGAAATG GAGTTTGCCA
    GCAGTTAATA TTTCGTGGCT CTTAGAAACT GCTAGAATGG GGAAGAGAGC AGATGAAAAC
    CATTTTCTGG TTGACAGTGC ACCTAAACAA GAACAAGTAT TAGAAACTAA CATACCAAAT
    GGAGTGAGTC CAAATCCAGA TTCTCCAGCA CATCCTGATG CACACTTGGA AATTCACAGA
    CAAAAGGCCG TTACACCTTT GGACATGAAC CGATTTCAGA GTAAAGCTTT CCATGCTGTC
    ATCTCACAGC ACAACAGGCA GGGCTTGGCC TCCCCACCAG TGAGACAGCC ACCTAAGAAG
    GAACCCTCCT TCCACCTGGA CACACCCTCA AAGTTCCTGT CCAAGGACAA GCTTTTCAAG
    CCATCCTTTG ATGTGACGGA TGCACTTGCA GCCTTAGAAA CCCCAAATGC TGCCAGCCAG
    AAGCGGAAAC TGAGCTCACC ACTCTCTGAA GTCATCGTCA GAAACCTGAC AACTGCTTTG
    GCAAATAGTT CACGAAACAC CGGCTGTCTT TCTGCTAGCC CTCAGCTAAA GGGTGCCCAT
    TTGGAGAAGG AAGAGACTCC GAAACCGCTT GACAAAGTTG TGGTGTGCGT GAGTAAAAGG
    CTCAGTAAGA AGCAGAGTGA GCTAAATGGG GTTGCAGCCT CACTGGGAGC AGAGTACAGG
    TGGAGTTTTG ATGAGACAGT TACTCATTAC ATTTACCAGG GTCGGGCAAA TGATAGTAAT
    CGCGAGTATA AATCTGCAAA GGAAAGAGGG GTGCACGTCG TGTCGGAGCA TTGGCTGTTA
    GAGTGTGCTC AAGAGTATAA GCATCTTCCA GAATCTCTTT ACCCACACAC TTACAACCCC
    AAAATGAGCT TGGACATCAG CACAGTGCAG GACGGGCGGC TCTGTGACAG CCGCTTACCC
    TCGGCACTTT CTGCATCTAA GGACGATGAG CCAGATCACC TGTCTGTAGA AGAAAATGCA
    ACAGAGACTT TGGGCACACA CAGCAGAGAG TCCGCTCTGC TGAATGGGAA TGGAAGGGAC
    GACTGCAAAG GAGCTCTGAC CCAGGCCTTG GAGATGAGAG AGAACTTCCA GAAACAACTA
    CAAGAGATCA TGTCAGCAAC CTCCATCGTG AGGACACCAG CCCAGAAGAC TTGCATGTCA
    AGAAGTAGTT GTAACAGCGC CTCTTCAACT CCTGACAGCA CTCGCTCTGC TCGGAGTGGA
    CGAAGCCGAG TCTTGGAGGC GCTCAGGCAG TCTCGACAGA TAGTGCCTGA TATCAACACA
    GAGCCCTCCC AGAATGAACA GATCATTTGG GATGACCCAA CAGCAAGGGA GGAGAGAGCA
    AGGCTTGCCA GCAACCTACA GTGGCCTAGC TACCCAACAC AGTATTCCGA GCTTCAGGTT
    GAGATAAAAA AACTGGATGA TTCTCCTTCT CGAAAGCCTG TGTACCATTC AGAAATTGCT
    GGGCAGGCCT CGTGTGTGAC TCAAGCCCCA GGGCAGCACC CAGGCTCTGA AGAACCAGAA
    GCTCCAGCAG CAGAAAGGAA TCTGATCCCT GAGCCTCAAG CCCCCAGCGT GGCTTTCCCC
    CTGGCTAAGC CGCCTGTAGC TCCACAACCT GAGGACAAGA TTGAAACACT GGAGGAGACG
    CATGGAAAAC TTAAAAAACA GTATGTGTTC CAGATGTCAT CTCTGAATTC TCAAGAGCGG
    ATTGATTACT GTCGCCTGAT TAAAGACCTA GGTGGGTCGG TGATCGAGAA GCAGTGCTCA
    GACCCCAGCT GCACACACAT GGTTGTGGGG TACCCACTGC GCAATGAGAA ATACTTAGCT
    TCCATGGCAG CTGGCAAATG GGTGCTTCAT CGCTCCTACC TGGACGCTTG CCGGACAGCT
    GGGCGCTTTG TGCAGGAGGA AGACTACGAG TGGGGAAGCA GCTCCATCCT AGATGCTCTC
    CCTGACGTCA CTGAGCACCA GCAGAAGCTC GCACTTGCAG CAATGAGATG GAGGAAAAGA
    ATCCAGCAGA GTCGGGAGTC GGGCATCGCT GAGGGAGCAT TTAGTGGCTG GAAAGTTATT
    TTGCGCGTTG ATCGGCCCCG AGAAGCAGGG TTTAAGCGCC TTCTTCAGGC AGGAGGAGCA
    AAGGTATTGC CTGGTCATCC AGAGCCTTTA TTTAAGGATG CTACACATCT CTTCTGTGAT
    TTCAATAAAC TGAAGCCTGA TGACTGCAGA GTTTTTATCG CAGAGGCTAC GGCCCAGAAC
    ATGGTCTGCT TGAAAACAGA ATATATTGCT GATTACCTCA TGATGGAATC CCCTCCTTGT
    GCTGATAATT ACCGAGTATC CGAAACTGCT TTATTCCGTA ACAAGGAAGG TGGGCCTGGA
    CTACCACAGA AGAGGAAAAC TCCTGCAGAG AACGTCATCA AACGGCCTCG GGTGCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001072207.4
    CDS205..4764
    Translation

    Target ORF information:

    RefSeq Version XM_001072207.5
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001072207.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCCAGAA ATGACCAAGA GCCGTTTTTT GTGAAGTTTT TAAAGTCTTC GGACAATTCA 
    GAATGTTTTT TTAAAGCTCT TGAGTCAATA AAGGAGTTAC AATCAGAAGA GTATCTTCAG
    GTTATTACCG ATGAGGAAGC ATTGAGGATT AAAGAGAATG ACAAATCGCT TTATATCTGT
    GACCGTTTCA GTGGCACTGC TTTTGATCAC CTTAAACAGC TTGGCTGCAG AATTGTTGGT
    CCTCAAGTAG TCACATTTTG TATGCGCCAC CAGCAGTGTG TCCCAAGAGC TGAACATCCA
    GTTTATAATA TGATTATGTC TGACATAACT ATATCTTGTA CAAGCCTGGA TAAAGACAAG
    AGGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT
    GTCTCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAATACTT AGTTGCTGCT
    AATCTGAAGA AACCTATTTT GCTTCCTGCT TGGATTAAAA CACTTTGGGA GAAGTCACAA
    GAAAAAAAAA TAACCAAATA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTC
    CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGTATAC ACAGGAAGAC AGTCCAGCAG
    CTCACAGCTA AGCATGGAGG CCAGTACATG GGGCAGCTGA AGATGAACGA GTGCACACAC
    CTCATTGTGC AAGAACCAAA AGGACAAAAA TATGAATGTG CCAGGAGATG GAATGTGCAC
    TGTGTGACCC TGCAATGGTT CCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAGTCT
    ATATACAAGG CCGAACCCAG AGCAGCGGTA AAGACGGTGC CTGATACTTC CACACCCACT
    GCTCAGAGCG GCAGTGCTGC AGCTGAGAAT CACAGGCTGG CAGATGTCAG CCATATATCC
    AACATCAACG GCAGCTGTGT AAATGAAACA ATGTTTGGTT CAACTACGAG CAAACTCGAA
    TGCACACTTG AGAACCTAGA AAATCTGGAC ATCAGTATGT TTCAAGCACC TGAAGATCTA
    TTAGATGGCT GTCGGATATA TCTTTGTGGC TTTAGTGGCA GAAAGCTCGA TAAGTTGAGA
    AGGCTTATTA ACAGTGGAGG TGGAGTTCGG TTTAATCAAC TCAATGAAGA TGTAACTCAT
    GTAATCATGG GAGATTATGA GGATGACGTG AGGCAGTTTT GGAGCAAATC CTCCCACAGG
    CCCCATGTAG TGGGAGCAAA GTGGTTGCTG GAGTGTTTTA GTAAAGGATA TATACTTCCT
    GAAGAGTCAT ATATCCACGC TAACTACCAG CCAGATGGAA TTCCAGTTTC AGATCAGCCC
    AGAAACCAGA CAACTGTCTT AGAAAAGAGT GGCACATTCT CTAAGAAGGA CTTTGTTCCT
    AATGAGAAGT TTCAGCAGAC TGATGAGGAT CTGCTTGCTC AGTATGTACA TGATGACTCC
    ACAATAGTTG AAGCTAAGCT ATCTGAAGCT ATAGAGCCTG AAGTTGGGCC TTGCGGTGGT
    TCTGCTCATG TTGAGCCCTG TGATGATTCC ACTCACATTT CTGTGCAAGA GGAAAACAAG
    TCGTCTGTCA GTCATTGCCT CCTTGATGGC TCTACAGTTC CTGAGGAAGG CTTATTTAGC
    CAAAAGAGTT TCCTTGTTTT GGGTTTTAGT GTTGAAAATG AATGTAATAT TGTAAACATC
    ATAAGAGAGC ATGCTGGAAA GATTGTGTCC CTTCCCAGCA GAATTGTTGC TGACTATGCC
    GTGGTTCCCC TGCTGGGATG TGAAGTAGAA GCGACAGTGG CAGAAGTTGT TACAAACACA
    TGGCTGGTTA CATGCATAGA CAACCAGACA TTAGTTGATC CCAAGTCGAA TCCCCTCTTC
    ACGCCAGTCT CAGTAATGTC AGGAATGACT CCTTTAGAGG ACTGTGTGAT TTCCTTCAGT
    CAGTGTGTGG GAGCAGAAAG AGATTCCTTG GTATTCTTAG CAAATCATCT TGGAGCAAGT
    GTTCAAGAAT TCTTTGTCCG CAAATCCAAT GCAAAGAAAG GCATGCTCGC CAGTACACAT
    CTTATAGTGA AAGAACCGAC TGGTTCTAAA TATGAAGCTG CAAAGAAATG GAGTTTGCCA
    GCAGTTAATA TTTCGTGGCT CTTAGAAACT GCTAGAATGG GGAAGAGAGC AGATGAAAAC
    CATTTTCTGG TTGACAGTGC ACCTAAACAA GAACAAGTAT TAGAAACTAA CATACCAAAT
    GGAGTGAGTC CAAATCCAGA TTCTCCAGCA CATCCTGATG CACACTTGGA AATTCACAGA
    CAAAAGGCCG TTACACCTTT GGACATGAAC CGATTTCAGA GTAAAGCTTT CCATGCTGTC
    ATCTCACAGC ACAACAGGCA GGGCTTGGCC TCCCCACCAG TGAGACAGCC ACCTAAGAAG
    GAACCCTCCT TCCACCTGGA CACACCCTCA AAGTTCCTGT CCAAGGACAA GCTTTTCAAG
    CCATCCTTTG ATGTGACGGA TGCACTTGCA GCCTTAGAAA CCCCAAATGC TGCCAGCCAG
    AAGCGGAAAC TGAGCTCACC ACTCTCTGAA GTCATCGTCA GAAACCTGAC AACTGCTTTG
    GCAAATAGTT CACGAAACAC CGGCTGTCTT TCTGCTAGCC CTCAGCTAAA GGGTGCCCAT
    TTGGAGAAGG AAGAGACTCC GAAACCGCTT GACAAAGTTG TGGTGTGCGT GAGTAAAAGG
    CTCAGTAAGA AGCAGAGTGA GCTAAATGGG GTTGCAGCCT CACTGGGAGC AGAGTACAGG
    TGGAGTTTTG ATGAGACAGT TACTCATTAC ATTTACCAGG GTCGGGCAAA TGATAGTAAT
    CGCGAGTATA AATCTGCAAA GGAAAGAGGG GTGCACGTCG TGTCGGAGCA TTGGCTGTTA
    GAGTGTGCTC AAGAGTATAA GCATCTTCCA GAATCTCTTT ACCCACACAC TTACAACCCC
    AAAATGAGCT TGGACATCAG CACAGTGCAG GACGGGCGGC TCTGTGACAG CCGCTTACCC
    TCGGCACTTT CTGCATCTAA GGACGATGAG CCAGATCACC TGTCTGTAGA AGAAAATGCA
    ACAGAGACTT TGGGCACACA CAGCAGAGAG TCCGCTCTGC TGAATGGGAA TGGAAGGGAC
    GACTGCAAAG GAGCTCTGAC CCAGGCCTTG GAGATGAGAG AGAACTTCCA GAAACAACTA
    CAAGAGATCA TGTCAGCAAC CTCCATCGTG AGGACACCAG CCCAGAAGAC TTGCATGTCA
    AGAAGTAGTT GTAACAGCGC CTCTTCAACT CCTGACAGCA CTCGCTCTGC TCGGAGTGGA
    CGAAGCCGAG TCTTGGAGGC GCTCAGGCAG TCTCGACAGA TAGTGCCTGA TATCAACACA
    GAGCCCTCCC AGAATGAACA GATCATTTGG GATGACCCAA CAGCAAGGGA GGAGAGAGCA
    AGGCTTGCCA GCAACCTACA GTGGCCTAGC TACCCAACAC AGTATTCCGA GCTTCAGGTT
    GAGATAAAAA AACTGGATGA TTCTCCTTCT CGAAAGCCTG TGTACCATTC AGAAATTGCT
    GGGCAGGCCT CGTGTGTGAC TCAAGCCCCA GGGCAGCACC CAGGCTCTGA AGAACCAGAA
    GCTCCAGCAG CAGAAAGGAA TCTGATCCCT GAGCCTCAAG CCCCCAGCGT GGCTTTCCCC
    CTGGCTAAGC CGCCTGTAGC TCCACAACCT GAGGACAAGA TTGAAACACT GGAGGAGACG
    CATGGAAAAC TTAAAAAACA GTATGTGTTC CAGATGTCAT CTCTGAATTC TCAAGAGCGG
    ATTGATTACT GTCGCCTGAT TAAAGACCTA GGTGGGTCGG TGATCGAGAA GCAGTGCTCA
    GACCCCAGCT GCACACACAT GGTTGTGGGG TACCCACTGC GCAATGAGAA ATACTTAGCT
    TCCATGGCAG CTGGCAAATG GGTGCTTCAT CGCTCCTACC TGGACGCTTG CCGGACAGCT
    GGGCGCTTTG TGCAGGAGGA AGACTACGAG TGGGGAAGCA GCTCCATCCT AGATGCTCTC
    CCTGACGTCA CTGAGCACCA GCAGAAGCTC GCACTTGCAG CAATGAGATG GAGGAAAAGA
    ATCCAGCAGA GTCGGGAGTC GGGCATCGCT GAGGGAGCAT TTAGTGGCTG GAAAGTTATT
    TTGCGCGTTG ATCGGCCCCG AGAAGCAGGG TTTAAGCGCC TTCTTCAGGC AGGAGGAGCA
    AAGGTATTGC CTGGTCATCC AGAGCCTTTA TTTAAGGATG CTACACATCT CTTCTGTGAT
    TTCAATAAAC TGAAGCCTGA TGACTGCAGA GTTTTTATCG CAGAGGCTAC GGCCCAGAAC
    ATGGTCTGCT TGAAAACAGA ATATATTGCT GATTACCTCA TGATGGAATC CCCTCCTTGT
    GCTGATAATT ACCGAGTATC CGAAACTGCT TTATTCCGTA ACAAGGAAGG TGGGCCTGGA
    CTACCACAGA AGAGGAAAAC TCCTGCAGAG AACGTCATCA AACGGCCTCG GGTGCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa47491
    Clone ID Related Accession (Same CDS sequence) XM_236578.8 , XM_001072207.5 , XM_008757855.2
    Accession Version XM_008757855.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4560bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product DNA topoisomerase 2-binding protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000076.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008757855.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCCAGAA ATGACCAAGA GCCGTTTTTT GTGAAGTTTT TAAAGTCTTC GGACAATTCA 
    GAATGTTTTT TTAAAGCTCT TGAGTCAATA AAGGAGTTAC AATCAGAAGA GTATCTTCAG
    GTTATTACCG ATGAGGAAGC ATTGAGGATT AAAGAGAATG ACAAATCGCT TTATATCTGT
    GACCGTTTCA GTGGCACTGC TTTTGATCAC CTTAAACAGC TTGGCTGCAG AATTGTTGGT
    CCTCAAGTAG TCACATTTTG TATGCGCCAC CAGCAGTGTG TCCCAAGAGC TGAACATCCA
    GTTTATAATA TGATTATGTC TGACATAACT ATATCTTGTA CAAGCCTGGA TAAAGACAAG
    AGGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT
    GTCTCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAATACTT AGTTGCTGCT
    AATCTGAAGA AACCTATTTT GCTTCCTGCT TGGATTAAAA CACTTTGGGA GAAGTCACAA
    GAAAAAAAAA TAACCAAATA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTC
    CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGTATAC ACAGGAAGAC AGTCCAGCAG
    CTCACAGCTA AGCATGGAGG CCAGTACATG GGGCAGCTGA AGATGAACGA GTGCACACAC
    CTCATTGTGC AAGAACCAAA AGGACAAAAA TATGAATGTG CCAGGAGATG GAATGTGCAC
    TGTGTGACCC TGCAATGGTT CCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAGTCT
    ATATACAAGG CCGAACCCAG AGCAGCGGTA AAGACGGTGC CTGATACTTC CACACCCACT
    GCTCAGAGCG GCAGTGCTGC AGCTGAGAAT CACAGGCTGG CAGATGTCAG CCATATATCC
    AACATCAACG GCAGCTGTGT AAATGAAACA ATGTTTGGTT CAACTACGAG CAAACTCGAA
    TGCACACTTG AGAACCTAGA AAATCTGGAC ATCAGTATGT TTCAAGCACC TGAAGATCTA
    TTAGATGGCT GTCGGATATA TCTTTGTGGC TTTAGTGGCA GAAAGCTCGA TAAGTTGAGA
    AGGCTTATTA ACAGTGGAGG TGGAGTTCGG TTTAATCAAC TCAATGAAGA TGTAACTCAT
    GTAATCATGG GAGATTATGA GGATGACGTG AGGCAGTTTT GGAGCAAATC CTCCCACAGG
    CCCCATGTAG TGGGAGCAAA GTGGTTGCTG GAGTGTTTTA GTAAAGGATA TATACTTCCT
    GAAGAGTCAT ATATCCACGC TAACTACCAG CCAGATGGAA TTCCAGTTTC AGATCAGCCC
    AGAAACCAGA CAACTGTCTT AGAAAAGAGT GGCACATTCT CTAAGAAGGA CTTTGTTCCT
    AATGAGAAGT TTCAGCAGAC TGATGAGGAT CTGCTTGCTC AGTATGTACA TGATGACTCC
    ACAATAGTTG AAGCTAAGCT ATCTGAAGCT ATAGAGCCTG AAGTTGGGCC TTGCGGTGGT
    TCTGCTCATG TTGAGCCCTG TGATGATTCC ACTCACATTT CTGTGCAAGA GGAAAACAAG
    TCGTCTGTCA GTCATTGCCT CCTTGATGGC TCTACAGTTC CTGAGGAAGG CTTATTTAGC
    CAAAAGAGTT TCCTTGTTTT GGGTTTTAGT GTTGAAAATG AATGTAATAT TGTAAACATC
    ATAAGAGAGC ATGCTGGAAA GATTGTGTCC CTTCCCAGCA GAATTGTTGC TGACTATGCC
    GTGGTTCCCC TGCTGGGATG TGAAGTAGAA GCGACAGTGG CAGAAGTTGT TACAAACACA
    TGGCTGGTTA CATGCATAGA CAACCAGACA TTAGTTGATC CCAAGTCGAA TCCCCTCTTC
    ACGCCAGTCT CAGTAATGTC AGGAATGACT CCTTTAGAGG ACTGTGTGAT TTCCTTCAGT
    CAGTGTGTGG GAGCAGAAAG AGATTCCTTG GTATTCTTAG CAAATCATCT TGGAGCAAGT
    GTTCAAGAAT TCTTTGTCCG CAAATCCAAT GCAAAGAAAG GCATGCTCGC CAGTACACAT
    CTTATAGTGA AAGAACCGAC TGGTTCTAAA TATGAAGCTG CAAAGAAATG GAGTTTGCCA
    GCAGTTAATA TTTCGTGGCT CTTAGAAACT GCTAGAATGG GGAAGAGAGC AGATGAAAAC
    CATTTTCTGG TTGACAGTGC ACCTAAACAA GAACAAGTAT TAGAAACTAA CATACCAAAT
    GGAGTGAGTC CAAATCCAGA TTCTCCAGCA CATCCTGATG CACACTTGGA AATTCACAGA
    CAAAAGGCCG TTACACCTTT GGACATGAAC CGATTTCAGA GTAAAGCTTT CCATGCTGTC
    ATCTCACAGC ACAACAGGCA GGGCTTGGCC TCCCCACCAG TGAGACAGCC ACCTAAGAAG
    GAACCCTCCT TCCACCTGGA CACACCCTCA AAGTTCCTGT CCAAGGACAA GCTTTTCAAG
    CCATCCTTTG ATGTGACGGA TGCACTTGCA GCCTTAGAAA CCCCAAATGC TGCCAGCCAG
    AAGCGGAAAC TGAGCTCACC ACTCTCTGAA GTCATCGTCA GAAACCTGAC AACTGCTTTG
    GCAAATAGTT CACGAAACAC CGGCTGTCTT TCTGCTAGCC CTCAGCTAAA GGGTGCCCAT
    TTGGAGAAGG AAGAGACTCC GAAACCGCTT GACAAAGTTG TGGTGTGCGT GAGTAAAAGG
    CTCAGTAAGA AGCAGAGTGA GCTAAATGGG GTTGCAGCCT CACTGGGAGC AGAGTACAGG
    TGGAGTTTTG ATGAGACAGT TACTCATTAC ATTTACCAGG GTCGGGCAAA TGATAGTAAT
    CGCGAGTATA AATCTGCAAA GGAAAGAGGG GTGCACGTCG TGTCGGAGCA TTGGCTGTTA
    GAGTGTGCTC AAGAGTATAA GCATCTTCCA GAATCTCTTT ACCCACACAC TTACAACCCC
    AAAATGAGCT TGGACATCAG CACAGTGCAG GACGGGCGGC TCTGTGACAG CCGCTTACCC
    TCGGCACTTT CTGCATCTAA GGACGATGAG CCAGATCACC TGTCTGTAGA AGAAAATGCA
    ACAGAGACTT TGGGCACACA CAGCAGAGAG TCCGCTCTGC TGAATGGGAA TGGAAGGGAC
    GACTGCAAAG GAGCTCTGAC CCAGGCCTTG GAGATGAGAG AGAACTTCCA GAAACAACTA
    CAAGAGATCA TGTCAGCAAC CTCCATCGTG AGGACACCAG CCCAGAAGAC TTGCATGTCA
    AGAAGTAGTT GTAACAGCGC CTCTTCAACT CCTGACAGCA CTCGCTCTGC TCGGAGTGGA
    CGAAGCCGAG TCTTGGAGGC GCTCAGGCAG TCTCGACAGA TAGTGCCTGA TATCAACACA
    GAGCCCTCCC AGAATGAACA GATCATTTGG GATGACCCAA CAGCAAGGGA GGAGAGAGCA
    AGGCTTGCCA GCAACCTACA GTGGCCTAGC TACCCAACAC AGTATTCCGA GCTTCAGGTT
    GAGATAAAAA AACTGGATGA TTCTCCTTCT CGAAAGCCTG TGTACCATTC AGAAATTGCT
    GGGCAGGCCT CGTGTGTGAC TCAAGCCCCA GGGCAGCACC CAGGCTCTGA AGAACCAGAA
    GCTCCAGCAG CAGAAAGGAA TCTGATCCCT GAGCCTCAAG CCCCCAGCGT GGCTTTCCCC
    CTGGCTAAGC CGCCTGTAGC TCCACAACCT GAGGACAAGA TTGAAACACT GGAGGAGACG
    CATGGAAAAC TTAAAAAACA GTATGTGTTC CAGATGTCAT CTCTGAATTC TCAAGAGCGG
    ATTGATTACT GTCGCCTGAT TAAAGACCTA GGTGGGTCGG TGATCGAGAA GCAGTGCTCA
    GACCCCAGCT GCACACACAT GGTTGTGGGG TACCCACTGC GCAATGAGAA ATACTTAGCT
    TCCATGGCAG CTGGCAAATG GGTGCTTCAT CGCTCCTACC TGGACGCTTG CCGGACAGCT
    GGGCGCTTTG TGCAGGAGGA AGACTACGAG TGGGGAAGCA GCTCCATCCT AGATGCTCTC
    CCTGACGTCA CTGAGCACCA GCAGAAGCTC GCACTTGCAG CAATGAGATG GAGGAAAAGA
    ATCCAGCAGA GTCGGGAGTC GGGCATCGCT GAGGGAGCAT TTAGTGGCTG GAAAGTTATT
    TTGCGCGTTG ATCGGCCCCG AGAAGCAGGG TTTAAGCGCC TTCTTCAGGC AGGAGGAGCA
    AAGGTATTGC CTGGTCATCC AGAGCCTTTA TTTAAGGATG CTACACATCT CTTCTGTGAT
    TTCAATAAAC TGAAGCCTGA TGACTGCAGA GTTTTTATCG CAGAGGCTAC GGCCCAGAAC
    ATGGTCTGCT TGAAAACAGA ATATATTGCT GATTACCTCA TGATGGAATC CCCTCCTTGT
    GCTGATAATT ACCGAGTATC CGAAACTGCT TTATTCCGTA ACAAGGAAGG TGGGCCTGGA
    CTACCACAGA AGAGGAAAAC TCCTGCAGAG AACGTCATCA AACGGCCTCG GGTGCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008756077.1
    CDS71..4630
    Translation

    Target ORF information:

    RefSeq Version XM_008757855.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008757855.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCCAGAA ATGACCAAGA GCCGTTTTTT GTGAAGTTTT TAAAGTCTTC GGACAATTCA 
    GAATGTTTTT TTAAAGCTCT TGAGTCAATA AAGGAGTTAC AATCAGAAGA GTATCTTCAG
    GTTATTACCG ATGAGGAAGC ATTGAGGATT AAAGAGAATG ACAAATCGCT TTATATCTGT
    GACCGTTTCA GTGGCACTGC TTTTGATCAC CTTAAACAGC TTGGCTGCAG AATTGTTGGT
    CCTCAAGTAG TCACATTTTG TATGCGCCAC CAGCAGTGTG TCCCAAGAGC TGAACATCCA
    GTTTATAATA TGATTATGTC TGACATAACT ATATCTTGTA CAAGCCTGGA TAAAGACAAG
    AGGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT
    GTCTCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAATACTT AGTTGCTGCT
    AATCTGAAGA AACCTATTTT GCTTCCTGCT TGGATTAAAA CACTTTGGGA GAAGTCACAA
    GAAAAAAAAA TAACCAAATA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTC
    CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGTATAC ACAGGAAGAC AGTCCAGCAG
    CTCACAGCTA AGCATGGAGG CCAGTACATG GGGCAGCTGA AGATGAACGA GTGCACACAC
    CTCATTGTGC AAGAACCAAA AGGACAAAAA TATGAATGTG CCAGGAGATG GAATGTGCAC
    TGTGTGACCC TGCAATGGTT CCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAGTCT
    ATATACAAGG CCGAACCCAG AGCAGCGGTA AAGACGGTGC CTGATACTTC CACACCCACT
    GCTCAGAGCG GCAGTGCTGC AGCTGAGAAT CACAGGCTGG CAGATGTCAG CCATATATCC
    AACATCAACG GCAGCTGTGT AAATGAAACA ATGTTTGGTT CAACTACGAG CAAACTCGAA
    TGCACACTTG AGAACCTAGA AAATCTGGAC ATCAGTATGT TTCAAGCACC TGAAGATCTA
    TTAGATGGCT GTCGGATATA TCTTTGTGGC TTTAGTGGCA GAAAGCTCGA TAAGTTGAGA
    AGGCTTATTA ACAGTGGAGG TGGAGTTCGG TTTAATCAAC TCAATGAAGA TGTAACTCAT
    GTAATCATGG GAGATTATGA GGATGACGTG AGGCAGTTTT GGAGCAAATC CTCCCACAGG
    CCCCATGTAG TGGGAGCAAA GTGGTTGCTG GAGTGTTTTA GTAAAGGATA TATACTTCCT
    GAAGAGTCAT ATATCCACGC TAACTACCAG CCAGATGGAA TTCCAGTTTC AGATCAGCCC
    AGAAACCAGA CAACTGTCTT AGAAAAGAGT GGCACATTCT CTAAGAAGGA CTTTGTTCCT
    AATGAGAAGT TTCAGCAGAC TGATGAGGAT CTGCTTGCTC AGTATGTACA TGATGACTCC
    ACAATAGTTG AAGCTAAGCT ATCTGAAGCT ATAGAGCCTG AAGTTGGGCC TTGCGGTGGT
    TCTGCTCATG TTGAGCCCTG TGATGATTCC ACTCACATTT CTGTGCAAGA GGAAAACAAG
    TCGTCTGTCA GTCATTGCCT CCTTGATGGC TCTACAGTTC CTGAGGAAGG CTTATTTAGC
    CAAAAGAGTT TCCTTGTTTT GGGTTTTAGT GTTGAAAATG AATGTAATAT TGTAAACATC
    ATAAGAGAGC ATGCTGGAAA GATTGTGTCC CTTCCCAGCA GAATTGTTGC TGACTATGCC
    GTGGTTCCCC TGCTGGGATG TGAAGTAGAA GCGACAGTGG CAGAAGTTGT TACAAACACA
    TGGCTGGTTA CATGCATAGA CAACCAGACA TTAGTTGATC CCAAGTCGAA TCCCCTCTTC
    ACGCCAGTCT CAGTAATGTC AGGAATGACT CCTTTAGAGG ACTGTGTGAT TTCCTTCAGT
    CAGTGTGTGG GAGCAGAAAG AGATTCCTTG GTATTCTTAG CAAATCATCT TGGAGCAAGT
    GTTCAAGAAT TCTTTGTCCG CAAATCCAAT GCAAAGAAAG GCATGCTCGC CAGTACACAT
    CTTATAGTGA AAGAACCGAC TGGTTCTAAA TATGAAGCTG CAAAGAAATG GAGTTTGCCA
    GCAGTTAATA TTTCGTGGCT CTTAGAAACT GCTAGAATGG GGAAGAGAGC AGATGAAAAC
    CATTTTCTGG TTGACAGTGC ACCTAAACAA GAACAAGTAT TAGAAACTAA CATACCAAAT
    GGAGTGAGTC CAAATCCAGA TTCTCCAGCA CATCCTGATG CACACTTGGA AATTCACAGA
    CAAAAGGCCG TTACACCTTT GGACATGAAC CGATTTCAGA GTAAAGCTTT CCATGCTGTC
    ATCTCACAGC ACAACAGGCA GGGCTTGGCC TCCCCACCAG TGAGACAGCC ACCTAAGAAG
    GAACCCTCCT TCCACCTGGA CACACCCTCA AAGTTCCTGT CCAAGGACAA GCTTTTCAAG
    CCATCCTTTG ATGTGACGGA TGCACTTGCA GCCTTAGAAA CCCCAAATGC TGCCAGCCAG
    AAGCGGAAAC TGAGCTCACC ACTCTCTGAA GTCATCGTCA GAAACCTGAC AACTGCTTTG
    GCAAATAGTT CACGAAACAC CGGCTGTCTT TCTGCTAGCC CTCAGCTAAA GGGTGCCCAT
    TTGGAGAAGG AAGAGACTCC GAAACCGCTT GACAAAGTTG TGGTGTGCGT GAGTAAAAGG
    CTCAGTAAGA AGCAGAGTGA GCTAAATGGG GTTGCAGCCT CACTGGGAGC AGAGTACAGG
    TGGAGTTTTG ATGAGACAGT TACTCATTAC ATTTACCAGG GTCGGGCAAA TGATAGTAAT
    CGCGAGTATA AATCTGCAAA GGAAAGAGGG GTGCACGTCG TGTCGGAGCA TTGGCTGTTA
    GAGTGTGCTC AAGAGTATAA GCATCTTCCA GAATCTCTTT ACCCACACAC TTACAACCCC
    AAAATGAGCT TGGACATCAG CACAGTGCAG GACGGGCGGC TCTGTGACAG CCGCTTACCC
    TCGGCACTTT CTGCATCTAA GGACGATGAG CCAGATCACC TGTCTGTAGA AGAAAATGCA
    ACAGAGACTT TGGGCACACA CAGCAGAGAG TCCGCTCTGC TGAATGGGAA TGGAAGGGAC
    GACTGCAAAG GAGCTCTGAC CCAGGCCTTG GAGATGAGAG AGAACTTCCA GAAACAACTA
    CAAGAGATCA TGTCAGCAAC CTCCATCGTG AGGACACCAG CCCAGAAGAC TTGCATGTCA
    AGAAGTAGTT GTAACAGCGC CTCTTCAACT CCTGACAGCA CTCGCTCTGC TCGGAGTGGA
    CGAAGCCGAG TCTTGGAGGC GCTCAGGCAG TCTCGACAGA TAGTGCCTGA TATCAACACA
    GAGCCCTCCC AGAATGAACA GATCATTTGG GATGACCCAA CAGCAAGGGA GGAGAGAGCA
    AGGCTTGCCA GCAACCTACA GTGGCCTAGC TACCCAACAC AGTATTCCGA GCTTCAGGTT
    GAGATAAAAA AACTGGATGA TTCTCCTTCT CGAAAGCCTG TGTACCATTC AGAAATTGCT
    GGGCAGGCCT CGTGTGTGAC TCAAGCCCCA GGGCAGCACC CAGGCTCTGA AGAACCAGAA
    GCTCCAGCAG CAGAAAGGAA TCTGATCCCT GAGCCTCAAG CCCCCAGCGT GGCTTTCCCC
    CTGGCTAAGC CGCCTGTAGC TCCACAACCT GAGGACAAGA TTGAAACACT GGAGGAGACG
    CATGGAAAAC TTAAAAAACA GTATGTGTTC CAGATGTCAT CTCTGAATTC TCAAGAGCGG
    ATTGATTACT GTCGCCTGAT TAAAGACCTA GGTGGGTCGG TGATCGAGAA GCAGTGCTCA
    GACCCCAGCT GCACACACAT GGTTGTGGGG TACCCACTGC GCAATGAGAA ATACTTAGCT
    TCCATGGCAG CTGGCAAATG GGTGCTTCAT CGCTCCTACC TGGACGCTTG CCGGACAGCT
    GGGCGCTTTG TGCAGGAGGA AGACTACGAG TGGGGAAGCA GCTCCATCCT AGATGCTCTC
    CCTGACGTCA CTGAGCACCA GCAGAAGCTC GCACTTGCAG CAATGAGATG GAGGAAAAGA
    ATCCAGCAGA GTCGGGAGTC GGGCATCGCT GAGGGAGCAT TTAGTGGCTG GAAAGTTATT
    TTGCGCGTTG ATCGGCCCCG AGAAGCAGGG TTTAAGCGCC TTCTTCAGGC AGGAGGAGCA
    AAGGTATTGC CTGGTCATCC AGAGCCTTTA TTTAAGGATG CTACACATCT CTTCTGTGAT
    TTCAATAAAC TGAAGCCTGA TGACTGCAGA GTTTTTATCG CAGAGGCTAC GGCCCAGAAC
    ATGGTCTGCT TGAAAACAGA ATATATTGCT GATTACCTCA TGATGGAATC CCCTCCTTGT
    GCTGATAATT ACCGAGTATC CGAAACTGCT TTATTCCGTA ACAAGGAAGG TGGGCCTGGA
    CTACCACAGA AGAGGAAAAC TCCTGCAGAG AACGTCATCA AACGGCCTCG GGTGCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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