Basp1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Gene Symbol	Basp1
Entrez Gene ID	64160
Full Name	brain abundant, membrane attached signal protein 1
Synonyms	NAP22
General protein information	Preferred Names brain abundant, membrane attached signal protein 1 Names brain acid soluble protein 1 22 kDa neuronal tissue-enriched acidic protein brain acidic membrane protein neuronal axonal membrane protein NAP-22
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q22

mRNA and Protein(s)

mRNA Protein Name

NM_022300.1 NP_071636.1 brain acid soluble protein 1
Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

[Amyloid-like oligomers of presynaptic protein BASP1].
Vitiuk OS, Gil'iano NIa, Zakharov VV
Rossiiskii fiziologicheskii zhurnal imeni I.M. Sechenova99(8)984-92(2013 Aug)

BASP1 and its N-end fragments (BNEMFs) dynamics in rat brain during development.
Kropotova E, Klementiev B, Mosevitsky M
Neurochemical research38(6)1278-84(2013 Jun)

Interaction of NAP-22 with brain glutamic acid decarboxylase (GAD).
Maekawa S, Kobayashi Y, Odagaki S, Makino M, Kumanogoh H, Nakamura S, Morita M, Hayashi F
Neuroscience letters53750-4(2013 Mar)

Quantitative proteomic analysis of human substantia nigra in Alzheimer's disease, Huntington's disease and Multiple sclerosis.
Chen S, Lu FF, Seeman P, Liu F
Neurochemical research37(12)2805-13(2012 Dec)

Motor, sensory and autonomic nerve terminals containing NAP-22 immunoreactivity in the rat muscle.
Iino S, Taguchi K, Maekawa S, Nojyo Y
Brain research1002(1-2)142-50(2004 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We conclude that BASP1 oligomer is a non-pathological physiologically relevant functional form of this protein.
Title: [Amyloid-like oligomers of presynaptic protein BASP1].

In rat brain, the amounts of all BASP1 forms per cell systematically increase during development and remain at the highest levels in adult animals.
Title: BASP1 and its N-end fragments (BNEMFs) dynamics in rat brain during development.

NAP-22 could participate in the transport of GAD65 and GAD67 to the presynaptic termini and their retention on the synaptic vesicles as an anchoring protein.
Title: Interaction of NAP-22 with brain glutamic acid decarboxylase (GAD).

In spermatozoa, as in chromatoid body, BASP1 and MARCKS may bind Ca(2+)-calmodulin and therefore contribute to the activation of calcium-dependent biochemical processes.
Title: Immunoelectron microscopic study of BASP1 and MARCKS location in the early and late rat spermatids.

Hence, the CapZ-NAP-22 complex could work as the nucleation site of actin polymerization or as the actin filament-anchoring site on the membrane microdomain.
Title: Biochemical interaction of an actin-capping protein, CapZ, with NAP-22.

mRNA	Protein	Name
NM_022300.1	NP_071636.1	brain acid soluble protein 1

The following Basp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Basp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05668	NM_022300.1 Latest version!	Rattus norvegicus brain abundant, membrane attached signal protein 1 (Basp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Basp1 ( NM_022300.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_071636.1 Rattus norvegicus brain abundant, membrane attached signal protein 1 (Basp1), mRNA.

Price & Availability↑

CloneID	ORa05668
Clone ID Related Accession (Same CDS sequence)	NM_022300.1
Accession Version	NM_022300.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	brain acid soluble protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D14441.1. On Jun 25, 2006 this sequence version replaced XM_001063291.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D14441.1, CB779780.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMEA2689603 [ECO:0000348] ##Evidence-Data-END##

ATGGGAAGCA AGCTGAGCAA GAAGAAGAAG GGCTACAATG TGAACGACGA GAAGGCCAAG 
GACAAAGACA AGAAGGCTGA AGGCGCGGGC ACCGAGGAGG AGGGGACCCA GAAGGAGAGC 
GAACCCCAGG CGGCCGCCGA CGCCACCGAG GTCAAGGAGA GCGCGGAGGA GAAGCCCAAG 
GACGCGGCGG ACGGCGAGGC CAAGGCGGAG GAAAAGGAGG CCGACAAGGC GGCGGCCAAG 
GAGGAGGCGC CCAAGGCCGA GCCCGAGAAG AGCGAGGGCG CCGCGGAGGA GCAGCCCGAG 
CCCGCGCCGG CGCCCGAGCA GGAGGCGGCC GCGCCCGGGC CCGCTGCGGG CGGCGAGGCC 
CCCAAGGCCG GCGAGGCGAG CGCGGAGAGC ACGGGCGCGG CCGACGGCGC GCCCCAGGAG 
GAGGGCGAGG CCAAAAAGAC TGAGGCTCCC GCCGCCGGCC CCGAAGCGAA AAGCGACGCG 
GCCCCGGCGG CTTCAGACTC TAAACCTAGC ACGGAGCCCG CGCCCTCGTC CAAGGAGACG 
CCCGCGGCCT CCGAGGCGCC CAGTTCCGCC GCCAAGGCCC CCGCGCCCGC CGCGCCTGCC 
GCCGAGCCCC AGGCCGAGGC ACCCGTCGCC AGCTCCGAGC AAAGCGTGGC CGTCAAAGAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071636.1
CDS	216..878
Translation	MGSKLSKKKKGYNVNDEKAKDKDKKAEGAGTEEEGTQKESEPQAAADATEVKESAEEKPKDAADGEAKAEEKEADKAAAKEEAPKAEPEKSEGAAEEQPEPAPAPEQEAAAPGPAAGGEAPKAGEASAESTGAADGAPQEEGEAKKTEAPAAGPEAKSDAAPAASDSKPSTEPAPSSKETPAASEAPSSAAKAPAPAAPAAEPQAEAPVASSEQSVAVKE

Target ORF information:

RefSeq Version	NM_022300.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus brain abundant, membrane attached signal protein 1 (Basp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022300.1

ORF Insert Sequence:

ATGGGAAGCA AGCTGAGCAA GAAGAAGAAG GGCTACAATG TGAACGACGA GAAGGCCAAG 
GACAAAGACA AGAAGGCTGA AGGCGCGGGC ACCGAGGAGG AGGGGACCCA GAAGGAGAGC 
GAACCCCAGG CGGCCGCCGA CGCCACCGAG GTCAAGGAGA GCGCGGAGGA GAAGCCCAAG 
GACGCGGCGG ACGGCGAGGC CAAGGCGGAG GAAAAGGAGG CCGACAAGGC GGCGGCCAAG 
GAGGAGGCGC CCAAGGCCGA GCCCGAGAAG AGCGAGGGCG CCGCGGAGGA GCAGCCCGAG 
CCCGCGCCGG CGCCCGAGCA GGAGGCGGCC GCGCCCGGGC CCGCTGCGGG CGGCGAGGCC 
CCCAAGGCCG GCGAGGCGAG CGCGGAGAGC ACGGGCGCGG CCGACGGCGC GCCCCAGGAG 
GAGGGCGAGG CCAAAAAGAC TGAGGCTCCC GCCGCCGGCC CCGAAGCGAA AAGCGACGCG 
GCCCCGGCGG CTTCAGACTC TAAACCTAGC ACGGAGCCCG CGCCCTCGTC CAAGGAGACG 
CCCGCGGCCT CCGAGGCGCC CAGTTCCGCC GCCAAGGCCC CCGCGCCCGC CGCGCCTGCC 
GCCGAGCCCC AGGCCGAGGC ACCCGTCGCC AGCTCCGAGC AAAGCGTGGC CGTCAAAGAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

[Amyloid-like oligomers of presynaptic protein BASP1].
Rossiiskii fiziologicheskii zhurnal imeni I.M. Sechenova99(8)984-92(2013 Aug)
Vitiuk OS,Gil'iano NIa,Zakharov VV

BASP1 and its N-end fragments (BNEMFs) dynamics in rat brain during development.
Neurochemical research38(6)1278-84(2013 Jun)
Kropotova E,Klementiev B,Mosevitsky M

Interaction of NAP-22 with brain glutamic acid decarboxylase (GAD).
Neuroscience letters53750-4(2013 Mar)
Maekawa S,Kobayashi Y,Odagaki S,Makino M,Kumanogoh H,Nakamura S,Morita M,Hayashi F

Quantitative proteomic analysis of human substantia nigra in Alzheimer's disease, Huntington's disease and Multiple sclerosis.
Neurochemical research37(12)2805-13(2012 Dec)
Chen S,Lu FF,Seeman P,Liu F

Motor, sensory and autonomic nerve terminals containing NAP-22 immunoreactivity in the rat muscle.
Brain research1002(1-2)142-50(2004 Mar)
Iino S,Taguchi K,Maekawa S,Nojyo Y

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Basp1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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