Necab1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Necab1
Entrez Gene ID	64169
Full Name	N-terminal EF-hand calcium binding protein 1
Synonyms	Efcbp1,Stip-1
General protein information	Preferred Names N-terminal EF-hand calcium binding protein 1 Names N-terminal EF-hand calcium-binding protein 1 EF-hand calcium-binding protein 1 neuronal Ca(2+)-binding protein 1 neuronal calcium-binding protein 1 synaptotagmin interacting protein 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q13
Summary	may play a role in neuronal Ca(2+)-signalling [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_022302.1	NP_071638.1	N-terminal EF-hand calcium-binding protein 1

Pathways from BioSystems

Homologies

	NECAB1	XP_001085626.1
Danio rerio (zebrafish)	necab1	NP_001017848.1
Homo sapiens (human)	NECAB1	NP_071746.1
Rattus norvegicus (Norway rat)	Necab1	NP_071638.1
Gallus gallus (chicken)	NECAB1	NP_001264342.1
Canis lupus familiaris (dog)	NECAB1	XP_005638213.1
Mus musculus (house mouse)	Necab1	NP_848732.3
Xenopus tropicalis (tropical clawed frog)	necab1	NP_001096422.1
Pan troglodytes (chimpanzee)	NECAB1	XP_528187.2

Gene Ontology
Bibliography

Related articles in PubMed

Towards Functional Annotation of the Preimplantation Transcriptome: An RNAi Screen in Mammalian Embryos.
Cui W, Dai X, Marcho C, Han Z, Zhang K, Tremblay KD, Mager J
Scientific reports637396(2016 Nov)

NECABs: a family of neuronal Ca(2+)-binding proteins with an unusual domain structure and a restricted expression pattern.
Sugita S, Ho A, S?dhof TC
Neuroscience112(1)51-63(2002)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Determines domain structure of the Necab 1 protein that expressed in the brain.
Title: NECABs: a family of neuronal Ca(2+)-binding proteins with an unusual domain structure and a restricted expression pattern.

The following Necab1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Necab1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05674	NM_022302.1 Latest version!	Rattus norvegicus N-terminal EF-hand calcium binding protein 1 (Necab1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Necab1 ( NM_022302.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_071638.1 Rattus norvegicus N-terminal EF-hand calcium binding protein 1 (Necab1), mRNA.

Price & Availability↑

CloneID	ORa05674
Clone ID Related Accession (Same CDS sequence)	NM_022302.1
Accession Version	NM_022302.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	N-terminal EF-hand calcium-binding protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF193755.1. ##Evidence-Data-START## Transcript exon combination :: AF193755.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGAGGATT CCCGGGAGAC CTCGCCGTCC TCCAACAACT CCTCAGAAGA GCTCAGCTCT 
GCCCTGCAGC TGTCCAAGGG CATGTCCATC TTCCTCGACA TACTGAGGAG AGCAGACAAA 
AATGACGATG GAAAATTGTC CTTTGAGGAA TTTAAAGCGT ATTTTGCAGA TGGAGTTCTC 
AGTGGAGAAG AACTACATGA GCTCTTCCAC ACCATTGACA CACACAATAC CAACAATCTT 
GACACAGAAG AACTATGTGA ATATTTTTCT CAGCACTTGG GTGAATATGA GAATGTTCTG 
GCTGCACTTG AAGACCTGAA TCTCTCCATC CTGAAAGCAA TGGGCAAGAC AAAGAAAGAC 
TACCAAGAAG CCTCCAATTT GGAACAATTT GTCACTCGAT TTTTGTTGAA GGAAACCTTG 
AATCAGTTGC AGTCTCTTCA GAACTCTCTG GAATGTGCCA TGGAAACTAC TGAAGAGCAA 
ACCCGTCAAG AAAGGCAAGG GCCATCCAAG CCAGAAGTCT TGTCCATTCA ATGGCCTGGA 
AAGCGATCCA GCCGCCGAGT CCAGAGACAC AACAGTTTCT CCCCCAACAG CCCACAGTTC 
AATGTCAGCA GCCCAGCATT GTTAGAAGAA GACAATCAGT GGATGACCCA GATAAACAGA 
CTCCAGAAAT TAATTGATAG ACTGGAAAAG AAGGATCTGA AACTTGAACC CCTGGAAGAA 
GAAGTTATTG AAGAGAACAC TAAACCTCAC ATCATGCTTG TGCAGCGCCA GATGTCTGTG 
ACAGAAGAGG ATCTGGAAGA ATTCCAGCTT GCTCTGAAGC ACTACGTGGA GAGCGCTTCA 
GCACAAAGTG GGTGCCTGCG TATCTCTATA CAGAAGCTTT CAAATGAATC TCGCTACATG 
ATTTATGAGT TCTGGGAGAA TAGCAGTGTG TGGAACCGCC ACCTTCAGAC AAATTACAGC 
AAGACGTTCC AAAGAAGCAA CGTGGATTTC TTGGAAACTC CAGAGCTCAC ATCTACTATG 
CTCGTTCCTG CTTCATGGTG GATCCTGAAA AACAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071638.1
CDS	76..1134
Translation	MEDSRETSPSSNNSSEELSSALQLSKGMSIFLDILRRADKNDDGKLSFEEFKAYFADGVLSGEELHELFHTIDTHNTNNLDTEELCEYFSQHLGEYENVLAALEDLNLSILKAMGKTKKDYQEASNLEQFVTRFLLKETLNQLQSLQNSLECAMETTEEQTRQERQGPSKPEVLSIQWPGKRSSRRVQRHNSFSPNSPQFNVSSPALLEEDNQWMTQINRLQKLIDRLEKKDLKLEPLEEEVIEENTKPHIMLVQRQMSVTEEDLEEFQLALKHYVESASAQSGCLRISIQKLSNESRYMIYEFWENSSVWNRHLQTNYSKTFQRSNVDFLETPELTSTMLVPASWWILKNN

Target ORF information:

RefSeq Version	NM_022302.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus N-terminal EF-hand calcium binding protein 1 (Necab1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022302.1

ORF Insert Sequence:

ATGGAGGATT CCCGGGAGAC CTCGCCGTCC TCCAACAACT CCTCAGAAGA GCTCAGCTCT 
GCCCTGCAGC TGTCCAAGGG CATGTCCATC TTCCTCGACA TACTGAGGAG AGCAGACAAA 
AATGACGATG GAAAATTGTC CTTTGAGGAA TTTAAAGCGT ATTTTGCAGA TGGAGTTCTC 
AGTGGAGAAG AACTACATGA GCTCTTCCAC ACCATTGACA CACACAATAC CAACAATCTT 
GACACAGAAG AACTATGTGA ATATTTTTCT CAGCACTTGG GTGAATATGA GAATGTTCTG 
GCTGCACTTG AAGACCTGAA TCTCTCCATC CTGAAAGCAA TGGGCAAGAC AAAGAAAGAC 
TACCAAGAAG CCTCCAATTT GGAACAATTT GTCACTCGAT TTTTGTTGAA GGAAACCTTG 
AATCAGTTGC AGTCTCTTCA GAACTCTCTG GAATGTGCCA TGGAAACTAC TGAAGAGCAA 
ACCCGTCAAG AAAGGCAAGG GCCATCCAAG CCAGAAGTCT TGTCCATTCA ATGGCCTGGA 
AAGCGATCCA GCCGCCGAGT CCAGAGACAC AACAGTTTCT CCCCCAACAG CCCACAGTTC 
AATGTCAGCA GCCCAGCATT GTTAGAAGAA GACAATCAGT GGATGACCCA GATAAACAGA 
CTCCAGAAAT TAATTGATAG ACTGGAAAAG AAGGATCTGA AACTTGAACC CCTGGAAGAA 
GAAGTTATTG AAGAGAACAC TAAACCTCAC ATCATGCTTG TGCAGCGCCA GATGTCTGTG 
ACAGAAGAGG ATCTGGAAGA ATTCCAGCTT GCTCTGAAGC ACTACGTGGA GAGCGCTTCA 
GCACAAAGTG GGTGCCTGCG TATCTCTATA CAGAAGCTTT CAAATGAATC TCGCTACATG 
ATTTATGAGT TCTGGGAGAA TAGCAGTGTG TGGAACCGCC ACCTTCAGAC AAATTACAGC 
AAGACGTTCC AAAGAAGCAA CGTGGATTTC TTGGAAACTC CAGAGCTCAC ATCTACTATG 
CTCGTTCCTG CTTCATGGTG GATCCTGAAA AACAATTAG