E4f1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Gene Symbol	E4f1
Entrez Gene ID	681359
Full Name	E4F transcription factor 1
Synonyms	Dnase1l2
General protein information	Preferred Names E4F transcription factor 1 Names transcription factor E4F1 deoxyribonuclease 1-like 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q12

mRNA and Protein(s)

mRNA	Protein	Name
XM_008767590.2	XP_008765812.1	transcription factor E4F1 isoform X2
XM_017597526.1	XP_017453015.1	transcription factor E4F1 isoform X5
NM_001185046.1	NP_001171975.1	transcription factor E4F1
XM_006246026.3	XP_006246088.1	transcription factor E4F1 isoform X1
XM_006246025.3	XP_006246087.1	transcription factor E4F1 isoform X1
XM_017597525.1	XP_017453014.1	transcription factor E4F1 isoform X3
XM_008767591.2	XP_008765813.1	transcription factor E4F1 isoform X4

Pathways from BioSystems

Homologies

Homo sapiens (human)	E4F1	NP_004415.3
Canis lupus familiaris (dog)	E4F1	XP_005622145.1
Gallus gallus (chicken)	E4F1	XP_414860.4
Xenopus tropicalis (tropical clawed frog)	e4f1	NP_001072166.1
Pan troglodytes (chimpanzee)	E4F1	XP_511205.3
Rattus norvegicus (Norway rat)	E4f1	NP_001171975.1
	E4F1	XP_001084651.1
Bos taurus (cattle)	E4F1	NP_001192754.1
Mus musculus (house mouse)	E4f1	NP_031919.2
Danio rerio (zebrafish)	e4f1	NP_001020684.1

Gene Ontology
Bibliography

Related articles in PubMed

The LIM-only protein FHL2 is a negative regulator of E4F1.
Paul C, Lacroix M, Iankova I, Julien E, Sch?fer BW, Labalette C, Wei Y, Le Cam A, Le Cam L, Sardet C
Oncogene25(40)5475-84(2006 Sep)

The E4F protein is required for mitotic progression during embryonic cell cycles.
Le Cam L, Lacroix M, Ciemerych MA, Sardet C, Sicinski P
Molecular and cellular biology24(14)6467-75(2004 Jul)

Transcriptional activation of the cyclin A gene by the architectural transcription factor HMGA2.
Tessari MA, Gostissa M, Altamura S, Sgarra R, Rustighi A, Salvagno C, Caretti G, Imbriano C, Mantovani R, Del Sal G, Giancotti V, Manfioletti G
Molecular and cellular biology23(24)9104-16(2003 Dec)

E4F and ATF, two transcription factors that recognize the same site, can be distinguished both physically and functionally: a role for E4F in E1A trans activation.
Rooney RJ, Raychaudhuri P, Nevins JR
Molecular and cellular biology10(10)5138-49(1990 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following E4f1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the E4f1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa06749	NM_001185046.1 Latest version!	Rattus norvegicus E4F transcription factor 1 (E4f1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa06749	XM_006246025.3 Latest version!	Rattus norvegicus E4F transcription factor 1 (E4f1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa06749	XM_006246026.3 Latest version!	Rattus norvegicus E4F transcription factor 1 (E4f1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa32916	XM_008767590.2 Latest version!	Rattus norvegicus E4F transcription factor 1 (E4f1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa32918	XM_008767591.2 Latest version!	Rattus norvegicus E4F transcription factor 1 (E4f1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa57474	XM_017597525.1 Latest version!	Rattus norvegicus E4F transcription factor 1 (E4f1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa57475	XM_017597526.1 Latest version!	Rattus norvegicus E4F transcription factor 1 (E4f1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

E4f1 ( NM_001185046.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001171975.1 Rattus norvegicus E4F transcription factor 1 (E4f1), mRNA.

Price & Availability↑

CloneID	ORa06749
Clone ID Related Accession (Same CDS sequence)	NM_001185046.1 , XM_006246026.3 , XM_006246025.3
Accession Version	NM_001185046.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Rattus norvegicus(Norway rat)
Product	transcription factor E4F1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CH473948.1. On or before May 30, 2010 this sequence version replaced XM_002724655.1, XM_001061399.3. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCGGGCCGAA 
GCCGGGCATG AAGCGGGCGA GGGCGGGGTT GCAGCGGCGG CGGCCTTGTC CAACGGCGGC 
TTTCTTGGGC TCCCAGCGCC CTTCAGCGAG GAAGATGAGG ATGACGTACA CAGATGTGGA 
CGCTGCCAAG TGGAATTCAC TGCCTTGGAG GACTTTGTCC AGCACAAGAT TCAGAAGACC 
TGCCATCGGG CCCCACAAGA GGCCCTGCCC ACCACCTCTG CTGCCACTTC GTTGCTGGGC 
CAGGAGGTGG TGCCAACAGC AGCAGAGGAA GGTCCAGAGG AACCCATCAC TGTGGCCCAC 
ATCGTGGTGG AGGCCACCTC TCTGGCAGAA GACATGAGCC ATGCTCCTGA CCTTGTTGGA 
AGTGGGCACA TCAAAGAGGT CATTGTGGCT GCTGAGGCAG AGCCAGGGGA TGGCGAGATG 
GCAGAGGCTC CAGGCAGTCC TAACCATCAG GAACTTGGGC TTATTGGGGA GGGCGAGCAG 
GCCCAAGTCA AGCTGCTGGT GAACAAGGAA GGCCGCTACG TGTGCATGCT ATGTCACAAG 
ACCTTCAAAA CGGGCAGCAT CCTCAAGGCT CACATGGTAA CACACAGCAG CCGCAAGGAC 
CACGAGTGCA AGCTCTGTGG GGCCTCCTTT CGGACCAAGG GCTCTCTGAT CCGGCACCAC 
CGACGGCACA CTGATGAGCG TCCCTACAAA TGTGCCAAGT GTGGGAAGAG TTTCCGAGAG 
TCAGGCGCAC TGACTCGGCA CCTCAAATCT CTCACTCCAT GCACAGAAAA GATCCGTTTC 
AGCATAAGCA AGGACACAGC CGTGGGCAAA GAGGAAGTGC CTGCAGGGTC CAGTGCCTCC 
ACTGTGGGGA CAGTGACATC GTCAGTGGCA GGAGAACCCA TGGAGACATC ACCTGTGATT 
CACCTGGTGA CAGATGCCAA GGGCACTGTC ATCCATGAAG TCCATGTCCA GATGCAGGAG 
CTTCCCCTGG GCATGAAAGC CCTGACCCCA GAGTCGGCAG ACTGTGAGGA GCTTCCCTGC 
TCCCGTGAGA ACAGCCGGGA GAACCTGCTA CATCAGGCCA TGCAGAATTC TGGCATCGTC 
CTCGACAGGG TTGCTGGGGA GGAGAGTACC CTGCAGCCAG CCCCTCCTTC TGGGTCCAGT 
CCCCAGTCCC TGGGAGATGG ACCCTCAGAG CTGCCACTGC TGGAGGTGGA GCAGATAGAG 
ACACAGGTAG CCAGTGAGGC AGCCACTGTG CCCAGGACCC ACCCGTGCTC TCAGTGCAGT 
GAGACTTTCC CATCAGCGGC TACCTTGGAG GCCCACAAGA GAGGTCATAT AGGGCCGAGG 
CCATTCACCT GCACACAGTG TGGCAAGGCC TTCCCCAAAG CCTACCTGCT CAAGAAGCAC 
CAGGAGGTGC ATGTGCACGA GCGCCGCTTC CGTTGTGGAG ACTGTGGGAA GCTTTATAAG 
ACCATTGCCC ATGTGCGGGG CCACCGGCGT GTCCACTCCG ACGAGAGGCC TTTCCCTTGT 
CCCCAGTGTG GCAAGCGTTA CAAGACCAAG AATGCCCAGC AAGTGCACTT CCGGACACAT 
TTGGAAGAAA AGCCCCACGT GTGCCAGTTC TGCAGCCGAG GCTTCCGGGA GAAGGGCTCT 
CTAGTGCGGC ATGTGAGGCA CCACACCGGC GAGAAACCTT TCAAGTGCTA CAAATGTGGC 
CGTGGCTTTG CAGAGCATGG CACACTCAAC CGGCACCTGC GCACTAAAGG GGGCTGCCTG 
CTCGAAGTGG AGGAGTTGCT GGTATCCGAG GAGAGCCCCT CTGCGGCTGC TACTGTGCTT 
GCAGAAGACC CCCACACGGT GCTGGTGGAG TTCTCATCTG TGGTGGCTGA TACCCAAGAG 
TACATTATTG AGGCTACTGC GGATGACACA GAGACCAGTG AAGCCACTGA GATCATTGAG 
GGCACGCAGA CAGAGGTGGA TAGTCACATC ATGAAGGTGG TCCAGCAGAT CGTGCACCAG 
GCTGGCGCTG GGCACCAGAT CATTGTGCAG AATGTGACCA TGGACCAGGA GGCAGCACTG 
GGCTCAGAGG CAGCTGCCGC GGACACGATC ACCATTGCCA CTCCTGAGAG TCTTACTGAG 
CAGGTGGCCA TGACGCTGGC TTCAGCCATC AGTGAGGGCA CTGTGCTCAC AGCCCGGGCA 
GGGCCAAACA GTACTGAACA GGCCACTGTG ACAATGGTGT CATCAGAGGA CATCGAGATC 
CTTGAGCACG GAGGGGAGCT GGTCATTGCT TCACCAGAGG GCCAGCTTGA GGTGCAGACA 
GTCATTGTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171975.1
CDS	82..2433
Translation	MEGAMAVRVTAAHTAEARAEAGHEAGEGGVAAAAALSNGGFLGLPAPFSEEDEDDVHRCGRCQVEFTALEDFVQHKIQKTCHRAPQEALPTTSAATSLLGQEVVPTAAEEGPEEPITVAHIVVEATSLAEDMSHAPDLVGSGHIKEVIVAAEAEPGDGEMAEAPGSPNHQELGLIGEGEQAQVKLLVNKEGRYVCMLCHKTFKTGSILKAHMVTHSSRKDHECKLCGASFRTKGSLIRHHRRHTDERPYKCAKCGKSFRESGALTRHLKSLTPCTEKIRFSISKDTAVGKEEVPAGSSASTVGTVTSSVAGEPMETSPVIHLVTDAKGTVIHEVHVQMQELPLGMKALTPESADCEELPCSRENSRENLLHQAMQNSGIVLDRVAGEESTLQPAPPSGSSPQSLGDGPSELPLLEVEQIETQVASEAATVPRTHPCSQCSETFPSAATLEAHKRGHIGPRPFTCTQCGKAFPKAYLLKKHQEVHVHERRFRCGDCGKLYKTIAHVRGHRRVHSDERPFPCPQCGKRYKTKNAQQVHFRTHLEEKPHVCQFCSRGFREKGSLVRHVRHHTGEKPFKCYKCGRGFAEHGTLNRHLRTKGGCLLEVEELLVSEESPSAAATVLAEDPHTVLVEFSSVVADTQEYIIEATADDTETSEATEIIEGTQTEVDSHIMKVVQQIVHQAGAGHQIIVQNVTMDQEAALGSEAAAADTITIATPESLTEQVAMTLASAISEGTVLTARAGPNSTEQATVTMVSSEDIEILEHGGELVIASPEGQLEVQTVIV

Target ORF information:

RefSeq Version	NM_001185046.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus E4F transcription factor 1 (E4f1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001185046.1

ORF Insert Sequence:

ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCGGGCCGAA 
GCCGGGCATG AAGCGGGCGA GGGCGGGGTT GCAGCGGCGG CGGCCTTGTC CAACGGCGGC 
TTTCTTGGGC TCCCAGCGCC CTTCAGCGAG GAAGATGAGG ATGACGTACA CAGATGTGGA 
CGCTGCCAAG TGGAATTCAC TGCCTTGGAG GACTTTGTCC AGCACAAGAT TCAGAAGACC 
TGCCATCGGG CCCCACAAGA GGCCCTGCCC ACCACCTCTG CTGCCACTTC GTTGCTGGGC 
CAGGAGGTGG TGCCAACAGC AGCAGAGGAA GGTCCAGAGG AACCCATCAC TGTGGCCCAC 
ATCGTGGTGG AGGCCACCTC TCTGGCAGAA GACATGAGCC ATGCTCCTGA CCTTGTTGGA 
AGTGGGCACA TCAAAGAGGT CATTGTGGCT GCTGAGGCAG AGCCAGGGGA TGGCGAGATG 
GCAGAGGCTC CAGGCAGTCC TAACCATCAG GAACTTGGGC TTATTGGGGA GGGCGAGCAG 
GCCCAAGTCA AGCTGCTGGT GAACAAGGAA GGCCGCTACG TGTGCATGCT ATGTCACAAG 
ACCTTCAAAA CGGGCAGCAT CCTCAAGGCT CACATGGTAA CACACAGCAG CCGCAAGGAC 
CACGAGTGCA AGCTCTGTGG GGCCTCCTTT CGGACCAAGG GCTCTCTGAT CCGGCACCAC 
CGACGGCACA CTGATGAGCG TCCCTACAAA TGTGCCAAGT GTGGGAAGAG TTTCCGAGAG 
TCAGGCGCAC TGACTCGGCA CCTCAAATCT CTCACTCCAT GCACAGAAAA GATCCGTTTC 
AGCATAAGCA AGGACACAGC CGTGGGCAAA GAGGAAGTGC CTGCAGGGTC CAGTGCCTCC 
ACTGTGGGGA CAGTGACATC GTCAGTGGCA GGAGAACCCA TGGAGACATC ACCTGTGATT 
CACCTGGTGA CAGATGCCAA GGGCACTGTC ATCCATGAAG TCCATGTCCA GATGCAGGAG 
CTTCCCCTGG GCATGAAAGC CCTGACCCCA GAGTCGGCAG ACTGTGAGGA GCTTCCCTGC 
TCCCGTGAGA ACAGCCGGGA GAACCTGCTA CATCAGGCCA TGCAGAATTC TGGCATCGTC 
CTCGACAGGG TTGCTGGGGA GGAGAGTACC CTGCAGCCAG CCCCTCCTTC TGGGTCCAGT 
CCCCAGTCCC TGGGAGATGG ACCCTCAGAG CTGCCACTGC TGGAGGTGGA GCAGATAGAG 
ACACAGGTAG CCAGTGAGGC AGCCACTGTG CCCAGGACCC ACCCGTGCTC TCAGTGCAGT 
GAGACTTTCC CATCAGCGGC TACCTTGGAG GCCCACAAGA GAGGTCATAT AGGGCCGAGG 
CCATTCACCT GCACACAGTG TGGCAAGGCC TTCCCCAAAG CCTACCTGCT CAAGAAGCAC 
CAGGAGGTGC ATGTGCACGA GCGCCGCTTC CGTTGTGGAG ACTGTGGGAA GCTTTATAAG 
ACCATTGCCC ATGTGCGGGG CCACCGGCGT GTCCACTCCG ACGAGAGGCC TTTCCCTTGT 
CCCCAGTGTG GCAAGCGTTA CAAGACCAAG AATGCCCAGC AAGTGCACTT CCGGACACAT 
TTGGAAGAAA AGCCCCACGT GTGCCAGTTC TGCAGCCGAG GCTTCCGGGA GAAGGGCTCT 
CTAGTGCGGC ATGTGAGGCA CCACACCGGC GAGAAACCTT TCAAGTGCTA CAAATGTGGC 
CGTGGCTTTG CAGAGCATGG CACACTCAAC CGGCACCTGC GCACTAAAGG GGGCTGCCTG 
CTCGAAGTGG AGGAGTTGCT GGTATCCGAG GAGAGCCCCT CTGCGGCTGC TACTGTGCTT 
GCAGAAGACC CCCACACGGT GCTGGTGGAG TTCTCATCTG TGGTGGCTGA TACCCAAGAG 
TACATTATTG AGGCTACTGC GGATGACACA GAGACCAGTG AAGCCACTGA GATCATTGAG 
GGCACGCAGA CAGAGGTGGA TAGTCACATC ATGAAGGTGG TCCAGCAGAT CGTGCACCAG 
GCTGGCGCTG GGCACCAGAT CATTGTGCAG AATGTGACCA TGGACCAGGA GGCAGCACTG 
GGCTCAGAGG CAGCTGCCGC GGACACGATC ACCATTGCCA CTCCTGAGAG TCTTACTGAG 
CAGGTGGCCA TGACGCTGGC TTCAGCCATC AGTGAGGGCA CTGTGCTCAC AGCCCGGGCA 
GGGCCAAACA GTACTGAACA GGCCACTGTG ACAATGGTGT CATCAGAGGA CATCGAGATC 
CTTGAGCACG GAGGGGAGCT GGTCATTGCT TCACCAGAGG GCCAGCTTGA GGTGCAGACA 
GTCATTGTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

E4f1 ( XM_006246026.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006246088.1 Rattus norvegicus E4F transcription factor 1 (E4f1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa06749
Clone ID Related Accession (Same CDS sequence)	NM_001185046.1 , XM_006246026.3 , XM_006246025.3
Accession Version	XM_006246026.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	transcription factor E4F1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006246026.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCGGGCCGAA 
GCCGGGCATG AAGCGGGCGA GGGCGGGGTT GCAGCGGCGG CGGCCTTGTC CAACGGCGGC 
TTTCTTGGGC TCCCAGCGCC CTTCAGCGAG GAAGATGAGG ATGACGTACA CAGATGTGGA 
CGCTGCCAAG TGGAATTCAC TGCCTTGGAG GACTTTGTCC AGCACAAGAT TCAGAAGACC 
TGCCATCGGG CCCCACAAGA GGCCCTGCCC ACCACCTCTG CTGCCACTTC GTTGCTGGGC 
CAGGAGGTGG TGCCAACAGC AGCAGAGGAA GGTCCAGAGG AACCCATCAC TGTGGCCCAC 
ATCGTGGTGG AGGCCACCTC TCTGGCAGAA GACATGAGCC ATGCTCCTGA CCTTGTTGGA 
AGTGGGCACA TCAAAGAGGT CATTGTGGCT GCTGAGGCAG AGCCAGGGGA TGGCGAGATG 
GCAGAGGCTC CAGGCAGTCC TAACCATCAG GAACTTGGGC TTATTGGGGA GGGCGAGCAG 
GCCCAAGTCA AGCTGCTGGT GAACAAGGAA GGCCGCTACG TGTGCATGCT ATGTCACAAG 
ACCTTCAAAA CGGGCAGCAT CCTCAAGGCT CACATGGTAA CACACAGCAG CCGCAAGGAC 
CACGAGTGCA AGCTCTGTGG GGCCTCCTTT CGGACCAAGG GCTCTCTGAT CCGGCACCAC 
CGACGGCACA CTGATGAGCG TCCCTACAAA TGTGCCAAGT GTGGGAAGAG TTTCCGAGAG 
TCAGGCGCAC TGACTCGGCA CCTCAAATCT CTCACTCCAT GCACAGAAAA GATCCGTTTC 
AGCATAAGCA AGGACACAGC CGTGGGCAAA GAGGAAGTGC CTGCAGGGTC CAGTGCCTCC 
ACTGTGGGGA CAGTGACATC GTCAGTGGCA GGAGAACCCA TGGAGACATC ACCTGTGATT 
CACCTGGTGA CAGATGCCAA GGGCACTGTC ATCCATGAAG TCCATGTCCA GATGCAGGAG 
CTTCCCCTGG GCATGAAAGC CCTGACCCCA GAGTCGGCAG ACTGTGAGGA GCTTCCCTGC 
TCCCGTGAGA ACAGCCGGGA GAACCTGCTA CATCAGGCCA TGCAGAATTC TGGCATCGTC 
CTCGACAGGG TTGCTGGGGA GGAGAGTACC CTGCAGCCAG CCCCTCCTTC TGGGTCCAGT 
CCCCAGTCCC TGGGAGATGG ACCCTCAGAG CTGCCACTGC TGGAGGTGGA GCAGATAGAG 
ACACAGGTAG CCAGTGAGGC AGCCACTGTG CCCAGGACCC ACCCGTGCTC TCAGTGCAGT 
GAGACTTTCC CATCAGCGGC TACCTTGGAG GCCCACAAGA GAGGTCATAT AGGGCCGAGG 
CCATTCACCT GCACACAGTG TGGCAAGGCC TTCCCCAAAG CCTACCTGCT CAAGAAGCAC 
CAGGAGGTGC ATGTGCACGA GCGCCGCTTC CGTTGTGGAG ACTGTGGGAA GCTTTATAAG 
ACCATTGCCC ATGTGCGGGG CCACCGGCGT GTCCACTCCG ACGAGAGGCC TTTCCCTTGT 
CCCCAGTGTG GCAAGCGTTA CAAGACCAAG AATGCCCAGC AAGTGCACTT CCGGACACAT 
TTGGAAGAAA AGCCCCACGT GTGCCAGTTC TGCAGCCGAG GCTTCCGGGA GAAGGGCTCT 
CTAGTGCGGC ATGTGAGGCA CCACACCGGC GAGAAACCTT TCAAGTGCTA CAAATGTGGC 
CGTGGCTTTG CAGAGCATGG CACACTCAAC CGGCACCTGC GCACTAAAGG GGGCTGCCTG 
CTCGAAGTGG AGGAGTTGCT GGTATCCGAG GAGAGCCCCT CTGCGGCTGC TACTGTGCTT 
GCAGAAGACC CCCACACGGT GCTGGTGGAG TTCTCATCTG TGGTGGCTGA TACCCAAGAG 
TACATTATTG AGGCTACTGC GGATGACACA GAGACCAGTG AAGCCACTGA GATCATTGAG 
GGCACGCAGA CAGAGGTGGA TAGTCACATC ATGAAGGTGG TCCAGCAGAT CGTGCACCAG 
GCTGGCGCTG GGCACCAGAT CATTGTGCAG AATGTGACCA TGGACCAGGA GGCAGCACTG 
GGCTCAGAGG CAGCTGCCGC GGACACGATC ACCATTGCCA CTCCTGAGAG TCTTACTGAG 
CAGGTGGCCA TGACGCTGGC TTCAGCCATC AGTGAGGGCA CTGTGCTCAC AGCCCGGGCA 
GGGCCAAACA GTACTGAACA GGCCACTGTG ACAATGGTGT CATCAGAGGA CATCGAGATC 
CTTGAGCACG GAGGGGAGCT GGTCATTGCT TCACCAGAGG GCCAGCTTGA GGTGCAGACA 
GTCATTGTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006246088.1
CDS	1135..3486
Translation	MEGAMAVRVTAAHTAEARAEAGHEAGEGGVAAAAALSNGGFLGLPAPFSEEDEDDVHRCGRCQVEFTALEDFVQHKIQKTCHRAPQEALPTTSAATSLLGQEVVPTAAEEGPEEPITVAHIVVEATSLAEDMSHAPDLVGSGHIKEVIVAAEAEPGDGEMAEAPGSPNHQELGLIGEGEQAQVKLLVNKEGRYVCMLCHKTFKTGSILKAHMVTHSSRKDHECKLCGASFRTKGSLIRHHRRHTDERPYKCAKCGKSFRESGALTRHLKSLTPCTEKIRFSISKDTAVGKEEVPAGSSASTVGTVTSSVAGEPMETSPVIHLVTDAKGTVIHEVHVQMQELPLGMKALTPESADCEELPCSRENSRENLLHQAMQNSGIVLDRVAGEESTLQPAPPSGSSPQSLGDGPSELPLLEVEQIETQVASEAATVPRTHPCSQCSETFPSAATLEAHKRGHIGPRPFTCTQCGKAFPKAYLLKKHQEVHVHERRFRCGDCGKLYKTIAHVRGHRRVHSDERPFPCPQCGKRYKTKNAQQVHFRTHLEEKPHVCQFCSRGFREKGSLVRHVRHHTGEKPFKCYKCGRGFAEHGTLNRHLRTKGGCLLEVEELLVSEESPSAAATVLAEDPHTVLVEFSSVVADTQEYIIEATADDTETSEATEIIEGTQTEVDSHIMKVVQQIVHQAGAGHQIIVQNVTMDQEAALGSEAAAADTITIATPESLTEQVAMTLASAISEGTVLTARAGPNSTEQATVTMVSSEDIEILEHGGELVIASPEGQLEVQTVIV

Target ORF information:

RefSeq Version	XM_006246026.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus E4F transcription factor 1 (E4f1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006246026.3

ORF Insert Sequence:

ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCGGGCCGAA 
GCCGGGCATG AAGCGGGCGA GGGCGGGGTT GCAGCGGCGG CGGCCTTGTC CAACGGCGGC 
TTTCTTGGGC TCCCAGCGCC CTTCAGCGAG GAAGATGAGG ATGACGTACA CAGATGTGGA 
CGCTGCCAAG TGGAATTCAC TGCCTTGGAG GACTTTGTCC AGCACAAGAT TCAGAAGACC 
TGCCATCGGG CCCCACAAGA GGCCCTGCCC ACCACCTCTG CTGCCACTTC GTTGCTGGGC 
CAGGAGGTGG TGCCAACAGC AGCAGAGGAA GGTCCAGAGG AACCCATCAC TGTGGCCCAC 
ATCGTGGTGG AGGCCACCTC TCTGGCAGAA GACATGAGCC ATGCTCCTGA CCTTGTTGGA 
AGTGGGCACA TCAAAGAGGT CATTGTGGCT GCTGAGGCAG AGCCAGGGGA TGGCGAGATG 
GCAGAGGCTC CAGGCAGTCC TAACCATCAG GAACTTGGGC TTATTGGGGA GGGCGAGCAG 
GCCCAAGTCA AGCTGCTGGT GAACAAGGAA GGCCGCTACG TGTGCATGCT ATGTCACAAG 
ACCTTCAAAA CGGGCAGCAT CCTCAAGGCT CACATGGTAA CACACAGCAG CCGCAAGGAC 
CACGAGTGCA AGCTCTGTGG GGCCTCCTTT CGGACCAAGG GCTCTCTGAT CCGGCACCAC 
CGACGGCACA CTGATGAGCG TCCCTACAAA TGTGCCAAGT GTGGGAAGAG TTTCCGAGAG 
TCAGGCGCAC TGACTCGGCA CCTCAAATCT CTCACTCCAT GCACAGAAAA GATCCGTTTC 
AGCATAAGCA AGGACACAGC CGTGGGCAAA GAGGAAGTGC CTGCAGGGTC CAGTGCCTCC 
ACTGTGGGGA CAGTGACATC GTCAGTGGCA GGAGAACCCA TGGAGACATC ACCTGTGATT 
CACCTGGTGA CAGATGCCAA GGGCACTGTC ATCCATGAAG TCCATGTCCA GATGCAGGAG 
CTTCCCCTGG GCATGAAAGC CCTGACCCCA GAGTCGGCAG ACTGTGAGGA GCTTCCCTGC 
TCCCGTGAGA ACAGCCGGGA GAACCTGCTA CATCAGGCCA TGCAGAATTC TGGCATCGTC 
CTCGACAGGG TTGCTGGGGA GGAGAGTACC CTGCAGCCAG CCCCTCCTTC TGGGTCCAGT 
CCCCAGTCCC TGGGAGATGG ACCCTCAGAG CTGCCACTGC TGGAGGTGGA GCAGATAGAG 
ACACAGGTAG CCAGTGAGGC AGCCACTGTG CCCAGGACCC ACCCGTGCTC TCAGTGCAGT 
GAGACTTTCC CATCAGCGGC TACCTTGGAG GCCCACAAGA GAGGTCATAT AGGGCCGAGG 
CCATTCACCT GCACACAGTG TGGCAAGGCC TTCCCCAAAG CCTACCTGCT CAAGAAGCAC 
CAGGAGGTGC ATGTGCACGA GCGCCGCTTC CGTTGTGGAG ACTGTGGGAA GCTTTATAAG 
ACCATTGCCC ATGTGCGGGG CCACCGGCGT GTCCACTCCG ACGAGAGGCC TTTCCCTTGT 
CCCCAGTGTG GCAAGCGTTA CAAGACCAAG AATGCCCAGC AAGTGCACTT CCGGACACAT 
TTGGAAGAAA AGCCCCACGT GTGCCAGTTC TGCAGCCGAG GCTTCCGGGA GAAGGGCTCT 
CTAGTGCGGC ATGTGAGGCA CCACACCGGC GAGAAACCTT TCAAGTGCTA CAAATGTGGC 
CGTGGCTTTG CAGAGCATGG CACACTCAAC CGGCACCTGC GCACTAAAGG GGGCTGCCTG 
CTCGAAGTGG AGGAGTTGCT GGTATCCGAG GAGAGCCCCT CTGCGGCTGC TACTGTGCTT 
GCAGAAGACC CCCACACGGT GCTGGTGGAG TTCTCATCTG TGGTGGCTGA TACCCAAGAG 
TACATTATTG AGGCTACTGC GGATGACACA GAGACCAGTG AAGCCACTGA GATCATTGAG 
GGCACGCAGA CAGAGGTGGA TAGTCACATC ATGAAGGTGG TCCAGCAGAT CGTGCACCAG 
GCTGGCGCTG GGCACCAGAT CATTGTGCAG AATGTGACCA TGGACCAGGA GGCAGCACTG 
GGCTCAGAGG CAGCTGCCGC GGACACGATC ACCATTGCCA CTCCTGAGAG TCTTACTGAG 
CAGGTGGCCA TGACGCTGGC TTCAGCCATC AGTGAGGGCA CTGTGCTCAC AGCCCGGGCA 
GGGCCAAACA GTACTGAACA GGCCACTGTG ACAATGGTGT CATCAGAGGA CATCGAGATC 
CTTGAGCACG GAGGGGAGCT GGTCATTGCT TCACCAGAGG GCCAGCTTGA GGTGCAGACA 
GTCATTGTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

E4f1 ( XM_006246025.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006246087.1 Rattus norvegicus E4F transcription factor 1 (E4f1), transcript variant X2, mRNA.

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CloneID	ORa06749
Clone ID Related Accession (Same CDS sequence)	NM_001185046.1 , XM_006246026.3 , XM_006246025.3
Accession Version	XM_006246025.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	transcription factor E4F1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006246025.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCGGGCCGAA 
GCCGGGCATG AAGCGGGCGA GGGCGGGGTT GCAGCGGCGG CGGCCTTGTC CAACGGCGGC 
TTTCTTGGGC TCCCAGCGCC CTTCAGCGAG GAAGATGAGG ATGACGTACA CAGATGTGGA 
CGCTGCCAAG TGGAATTCAC TGCCTTGGAG GACTTTGTCC AGCACAAGAT TCAGAAGACC 
TGCCATCGGG CCCCACAAGA GGCCCTGCCC ACCACCTCTG CTGCCACTTC GTTGCTGGGC 
CAGGAGGTGG TGCCAACAGC AGCAGAGGAA GGTCCAGAGG AACCCATCAC TGTGGCCCAC 
ATCGTGGTGG AGGCCACCTC TCTGGCAGAA GACATGAGCC ATGCTCCTGA CCTTGTTGGA 
AGTGGGCACA TCAAAGAGGT CATTGTGGCT GCTGAGGCAG AGCCAGGGGA TGGCGAGATG 
GCAGAGGCTC CAGGCAGTCC TAACCATCAG GAACTTGGGC TTATTGGGGA GGGCGAGCAG 
GCCCAAGTCA AGCTGCTGGT GAACAAGGAA GGCCGCTACG TGTGCATGCT ATGTCACAAG 
ACCTTCAAAA CGGGCAGCAT CCTCAAGGCT CACATGGTAA CACACAGCAG CCGCAAGGAC 
CACGAGTGCA AGCTCTGTGG GGCCTCCTTT CGGACCAAGG GCTCTCTGAT CCGGCACCAC 
CGACGGCACA CTGATGAGCG TCCCTACAAA TGTGCCAAGT GTGGGAAGAG TTTCCGAGAG 
TCAGGCGCAC TGACTCGGCA CCTCAAATCT CTCACTCCAT GCACAGAAAA GATCCGTTTC 
AGCATAAGCA AGGACACAGC CGTGGGCAAA GAGGAAGTGC CTGCAGGGTC CAGTGCCTCC 
ACTGTGGGGA CAGTGACATC GTCAGTGGCA GGAGAACCCA TGGAGACATC ACCTGTGATT 
CACCTGGTGA CAGATGCCAA GGGCACTGTC ATCCATGAAG TCCATGTCCA GATGCAGGAG 
CTTCCCCTGG GCATGAAAGC CCTGACCCCA GAGTCGGCAG ACTGTGAGGA GCTTCCCTGC 
TCCCGTGAGA ACAGCCGGGA GAACCTGCTA CATCAGGCCA TGCAGAATTC TGGCATCGTC 
CTCGACAGGG TTGCTGGGGA GGAGAGTACC CTGCAGCCAG CCCCTCCTTC TGGGTCCAGT 
CCCCAGTCCC TGGGAGATGG ACCCTCAGAG CTGCCACTGC TGGAGGTGGA GCAGATAGAG 
ACACAGGTAG CCAGTGAGGC AGCCACTGTG CCCAGGACCC ACCCGTGCTC TCAGTGCAGT 
GAGACTTTCC CATCAGCGGC TACCTTGGAG GCCCACAAGA GAGGTCATAT AGGGCCGAGG 
CCATTCACCT GCACACAGTG TGGCAAGGCC TTCCCCAAAG CCTACCTGCT CAAGAAGCAC 
CAGGAGGTGC ATGTGCACGA GCGCCGCTTC CGTTGTGGAG ACTGTGGGAA GCTTTATAAG 
ACCATTGCCC ATGTGCGGGG CCACCGGCGT GTCCACTCCG ACGAGAGGCC TTTCCCTTGT 
CCCCAGTGTG GCAAGCGTTA CAAGACCAAG AATGCCCAGC AAGTGCACTT CCGGACACAT 
TTGGAAGAAA AGCCCCACGT GTGCCAGTTC TGCAGCCGAG GCTTCCGGGA GAAGGGCTCT 
CTAGTGCGGC ATGTGAGGCA CCACACCGGC GAGAAACCTT TCAAGTGCTA CAAATGTGGC 
CGTGGCTTTG CAGAGCATGG CACACTCAAC CGGCACCTGC GCACTAAAGG GGGCTGCCTG 
CTCGAAGTGG AGGAGTTGCT GGTATCCGAG GAGAGCCCCT CTGCGGCTGC TACTGTGCTT 
GCAGAAGACC CCCACACGGT GCTGGTGGAG TTCTCATCTG TGGTGGCTGA TACCCAAGAG 
TACATTATTG AGGCTACTGC GGATGACACA GAGACCAGTG AAGCCACTGA GATCATTGAG 
GGCACGCAGA CAGAGGTGGA TAGTCACATC ATGAAGGTGG TCCAGCAGAT CGTGCACCAG 
GCTGGCGCTG GGCACCAGAT CATTGTGCAG AATGTGACCA TGGACCAGGA GGCAGCACTG 
GGCTCAGAGG CAGCTGCCGC GGACACGATC ACCATTGCCA CTCCTGAGAG TCTTACTGAG 
CAGGTGGCCA TGACGCTGGC TTCAGCCATC AGTGAGGGCA CTGTGCTCAC AGCCCGGGCA 
GGGCCAAACA GTACTGAACA GGCCACTGTG ACAATGGTGT CATCAGAGGA CATCGAGATC 
CTTGAGCACG GAGGGGAGCT GGTCATTGCT TCACCAGAGG GCCAGCTTGA GGTGCAGACA 
GTCATTGTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006246087.1
CDS	1135..3486
Translation	MEGAMAVRVTAAHTAEARAEAGHEAGEGGVAAAAALSNGGFLGLPAPFSEEDEDDVHRCGRCQVEFTALEDFVQHKIQKTCHRAPQEALPTTSAATSLLGQEVVPTAAEEGPEEPITVAHIVVEATSLAEDMSHAPDLVGSGHIKEVIVAAEAEPGDGEMAEAPGSPNHQELGLIGEGEQAQVKLLVNKEGRYVCMLCHKTFKTGSILKAHMVTHSSRKDHECKLCGASFRTKGSLIRHHRRHTDERPYKCAKCGKSFRESGALTRHLKSLTPCTEKIRFSISKDTAVGKEEVPAGSSASTVGTVTSSVAGEPMETSPVIHLVTDAKGTVIHEVHVQMQELPLGMKALTPESADCEELPCSRENSRENLLHQAMQNSGIVLDRVAGEESTLQPAPPSGSSPQSLGDGPSELPLLEVEQIETQVASEAATVPRTHPCSQCSETFPSAATLEAHKRGHIGPRPFTCTQCGKAFPKAYLLKKHQEVHVHERRFRCGDCGKLYKTIAHVRGHRRVHSDERPFPCPQCGKRYKTKNAQQVHFRTHLEEKPHVCQFCSRGFREKGSLVRHVRHHTGEKPFKCYKCGRGFAEHGTLNRHLRTKGGCLLEVEELLVSEESPSAAATVLAEDPHTVLVEFSSVVADTQEYIIEATADDTETSEATEIIEGTQTEVDSHIMKVVQQIVHQAGAGHQIIVQNVTMDQEAALGSEAAAADTITIATPESLTEQVAMTLASAISEGTVLTARAGPNSTEQATVTMVSSEDIEILEHGGELVIASPEGQLEVQTVIV

Target ORF information:

RefSeq Version	XM_006246025.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus E4F transcription factor 1 (E4f1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006246025.3

ORF Insert Sequence:

ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCGGGCCGAA 
GCCGGGCATG AAGCGGGCGA GGGCGGGGTT GCAGCGGCGG CGGCCTTGTC CAACGGCGGC 
TTTCTTGGGC TCCCAGCGCC CTTCAGCGAG GAAGATGAGG ATGACGTACA CAGATGTGGA 
CGCTGCCAAG TGGAATTCAC TGCCTTGGAG GACTTTGTCC AGCACAAGAT TCAGAAGACC 
TGCCATCGGG CCCCACAAGA GGCCCTGCCC ACCACCTCTG CTGCCACTTC GTTGCTGGGC 
CAGGAGGTGG TGCCAACAGC AGCAGAGGAA GGTCCAGAGG AACCCATCAC TGTGGCCCAC 
ATCGTGGTGG AGGCCACCTC TCTGGCAGAA GACATGAGCC ATGCTCCTGA CCTTGTTGGA 
AGTGGGCACA TCAAAGAGGT CATTGTGGCT GCTGAGGCAG AGCCAGGGGA TGGCGAGATG 
GCAGAGGCTC CAGGCAGTCC TAACCATCAG GAACTTGGGC TTATTGGGGA GGGCGAGCAG 
GCCCAAGTCA AGCTGCTGGT GAACAAGGAA GGCCGCTACG TGTGCATGCT ATGTCACAAG 
ACCTTCAAAA CGGGCAGCAT CCTCAAGGCT CACATGGTAA CACACAGCAG CCGCAAGGAC 
CACGAGTGCA AGCTCTGTGG GGCCTCCTTT CGGACCAAGG GCTCTCTGAT CCGGCACCAC 
CGACGGCACA CTGATGAGCG TCCCTACAAA TGTGCCAAGT GTGGGAAGAG TTTCCGAGAG 
TCAGGCGCAC TGACTCGGCA CCTCAAATCT CTCACTCCAT GCACAGAAAA GATCCGTTTC 
AGCATAAGCA AGGACACAGC CGTGGGCAAA GAGGAAGTGC CTGCAGGGTC CAGTGCCTCC 
ACTGTGGGGA CAGTGACATC GTCAGTGGCA GGAGAACCCA TGGAGACATC ACCTGTGATT 
CACCTGGTGA CAGATGCCAA GGGCACTGTC ATCCATGAAG TCCATGTCCA GATGCAGGAG 
CTTCCCCTGG GCATGAAAGC CCTGACCCCA GAGTCGGCAG ACTGTGAGGA GCTTCCCTGC 
TCCCGTGAGA ACAGCCGGGA GAACCTGCTA CATCAGGCCA TGCAGAATTC TGGCATCGTC 
CTCGACAGGG TTGCTGGGGA GGAGAGTACC CTGCAGCCAG CCCCTCCTTC TGGGTCCAGT 
CCCCAGTCCC TGGGAGATGG ACCCTCAGAG CTGCCACTGC TGGAGGTGGA GCAGATAGAG 
ACACAGGTAG CCAGTGAGGC AGCCACTGTG CCCAGGACCC ACCCGTGCTC TCAGTGCAGT 
GAGACTTTCC CATCAGCGGC TACCTTGGAG GCCCACAAGA GAGGTCATAT AGGGCCGAGG 
CCATTCACCT GCACACAGTG TGGCAAGGCC TTCCCCAAAG CCTACCTGCT CAAGAAGCAC 
CAGGAGGTGC ATGTGCACGA GCGCCGCTTC CGTTGTGGAG ACTGTGGGAA GCTTTATAAG 
ACCATTGCCC ATGTGCGGGG CCACCGGCGT GTCCACTCCG ACGAGAGGCC TTTCCCTTGT 
CCCCAGTGTG GCAAGCGTTA CAAGACCAAG AATGCCCAGC AAGTGCACTT CCGGACACAT 
TTGGAAGAAA AGCCCCACGT GTGCCAGTTC TGCAGCCGAG GCTTCCGGGA GAAGGGCTCT 
CTAGTGCGGC ATGTGAGGCA CCACACCGGC GAGAAACCTT TCAAGTGCTA CAAATGTGGC 
CGTGGCTTTG CAGAGCATGG CACACTCAAC CGGCACCTGC GCACTAAAGG GGGCTGCCTG 
CTCGAAGTGG AGGAGTTGCT GGTATCCGAG GAGAGCCCCT CTGCGGCTGC TACTGTGCTT 
GCAGAAGACC CCCACACGGT GCTGGTGGAG TTCTCATCTG TGGTGGCTGA TACCCAAGAG 
TACATTATTG AGGCTACTGC GGATGACACA GAGACCAGTG AAGCCACTGA GATCATTGAG 
GGCACGCAGA CAGAGGTGGA TAGTCACATC ATGAAGGTGG TCCAGCAGAT CGTGCACCAG 
GCTGGCGCTG GGCACCAGAT CATTGTGCAG AATGTGACCA TGGACCAGGA GGCAGCACTG 
GGCTCAGAGG CAGCTGCCGC GGACACGATC ACCATTGCCA CTCCTGAGAG TCTTACTGAG 
CAGGTGGCCA TGACGCTGGC TTCAGCCATC AGTGAGGGCA CTGTGCTCAC AGCCCGGGCA 
GGGCCAAACA GTACTGAACA GGCCACTGTG ACAATGGTGT CATCAGAGGA CATCGAGATC 
CTTGAGCACG GAGGGGAGCT GGTCATTGCT TCACCAGAGG GCCAGCTTGA GGTGCAGACA 
GTCATTGTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

E4f1 ( XM_008767590.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008765812.1 Rattus norvegicus E4F transcription factor 1 (E4f1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa32916
Clone ID Related Accession (Same CDS sequence)	XM_008767590.2
Accession Version	XM_008767590.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	transcription factor E4F1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767590.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCGGGCCGAA 
GCCGGGCATG AAGCGGGCGA GGGCGGGGTT GCAGCGGCGG CGGCCTTGTC CAACGGCGGC 
TTTCTTGGGC TCCCAGCGCC CTTCAGCGAG GAAGATGAGG ATGACGTACA CAGATGTGGA 
CGCTGCCAAG TGGAATTCAC TGCCTTGGAG GACTTTGTCC AGCACAAGAT TCAGAAGACC 
TGCCATCGGG CCCCACAAGA GGCCCTGCCC ACCACCTCTG CTGCCACTTC GTTGCTGGGC 
CAGGAGGTGG TGCCAACAGC AGCAGAGGAA GGTCCAGAGG AACCCATCAC TGTGGCCCAC 
ATCGTGGTGG AGGCCACCTC TCTGGCAGAA GACATGAGCC ATGCTCCTGA CCTTGTTGGA 
AGTGGGCACA TCAAAGAGGT CATTGTGGCT GCTGAGGCAG AGCCAGGGGA TGGCGAGATG 
GCAGAGGCTC CAGGCAGTCC TAACCATCAG GAACTTGGGC TTATTGGGGA GGGCGAGCAG 
GCCCAAGTCA AGCTGCTGGT GAACAAGGAA GGCCGCTACG TGTGCATGCT ATGTCACAAG 
ACCTTCAAAA CGGGCAGCAT CCTCAAGGCT CACATGGTAA CACACAGCAG CCGCAAGGAC 
CACGAGTGCA AGCTCTGTGG GGCCTCCTTT CGGACCAAGG GCTCTCTGAT CCGGCACCAC 
CGACGGCACA CTGATGAGCG TCCCTACAAA TGTGCCAAGT GTGGGAAGAG TTTCCGAGAG 
TCAGGCGCAC TGACTCGGCA CCTCAAATCT CTCACTCCAT GCACAGAAAA GATCCGTTTC 
AGCATAAGCA AGGACACAGC CGTGGGCAAA GAGGAAGTGC CTGCAGGGTC CAGTGCCTCC 
ACTGTGGGGA CAGTGACATC GTCAGTGGCA GGAGAACCCA TGGAGACATC ACCTGTGATT 
CACCTGGTGA CAGATGCCAA GGGCACTGTC ATCCATGAAG TCCATGTCCA GATGCAGGAG 
CTTCCCCTGG GCATGAAAGC CCTGACCCCA GAGTCGGCAG ACTGTGAGGA GCTTCCCTGC 
TCCCGTGAGA ACAGCCGGGA GAACCTGCTA CATCAGGCCA TGCAGAATTC TGGCATCGTC 
CTCGACAGGG TTGCTGGGGA GGAGAGTACC CTGCAGCCAG CCCCTCCTTC TGGGTCCAGT 
CCCCAGTCCC TGGGAGATGG ACCCTCAGAG CTGCCACTGC TGGAGGTGGA GCAGATAGAG 
ACAGTAGCCA GTGAGGCAGC CACTGTGCCC AGGACCCACC CGTGCTCTCA GTGCAGTGAG 
ACTTTCCCAT CAGCGGCTAC CTTGGAGGCC CACAAGAGAG GTCATATAGG GCCGAGGCCA 
TTCACCTGCA CACAGTGTGG CAAGGCCTTC CCCAAAGCCT ACCTGCTCAA GAAGCACCAG 
GAGGTGCATG TGCACGAGCG CCGCTTCCGT TGTGGAGACT GTGGGAAGCT TTATAAGACC 
ATTGCCCATG TGCGGGGCCA CCGGCGTGTC CACTCCGACG AGAGGCCTTT CCCTTGTCCC 
CAGTGTGGCA AGCGTTACAA GACCAAGAAT GCCCAGCAAG TGCACTTCCG GACACATTTG 
GAAGAAAAGC CCCACGTGTG CCAGTTCTGC AGCCGAGGCT TCCGGGAGAA GGGCTCTCTA 
GTGCGGCATG TGAGGCACCA CACCGGCGAG AAACCTTTCA AGTGCTACAA ATGTGGCCGT 
GGCTTTGCAG AGCATGGCAC ACTCAACCGG CACCTGCGCA CTAAAGGGGG CTGCCTGCTC 
GAAGTGGAGG AGTTGCTGGT ATCCGAGGAG AGCCCCTCTG CGGCTGCTAC TGTGCTTGCA 
GAAGACCCCC ACACGGTGCT GGTGGAGTTC TCATCTGTGG TGGCTGATAC CCAAGAGTAC 
ATTATTGAGG CTACTGCGGA TGACACAGAG ACCAGTGAAG CCACTGAGAT CATTGAGGGC 
ACGCAGACAG AGGTGGATAG TCACATCATG AAGGTGGTCC AGCAGATCGT GCACCAGGCT 
GGCGCTGGGC ACCAGATCAT TGTGCAGAAT GTGACCATGG ACCAGGAGGC AGCACTGGGC 
TCAGAGGCAG CTGCCGCGGA CACGATCACC ATTGCCACTC CTGAGAGTCT TACTGAGCAG 
GTGGCCATGA CGCTGGCTTC AGCCATCAGT GAGGGCACTG TGCTCACAGC CCGGGCAGGG 
CCAAACAGTA CTGAACAGGC CACTGTGACA ATGGTGTCAT CAGAGGACAT CGAGATCCTT 
GAGCACGGAG GGGAGCTGGT CATTGCTTCA CCAGAGGGCC AGCTTGAGGT GCAGACAGTC 
ATTGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008765812.1
CDS	1135..3483
Translation	MEGAMAVRVTAAHTAEARAEAGHEAGEGGVAAAAALSNGGFLGLPAPFSEEDEDDVHRCGRCQVEFTALEDFVQHKIQKTCHRAPQEALPTTSAATSLLGQEVVPTAAEEGPEEPITVAHIVVEATSLAEDMSHAPDLVGSGHIKEVIVAAEAEPGDGEMAEAPGSPNHQELGLIGEGEQAQVKLLVNKEGRYVCMLCHKTFKTGSILKAHMVTHSSRKDHECKLCGASFRTKGSLIRHHRRHTDERPYKCAKCGKSFRESGALTRHLKSLTPCTEKIRFSISKDTAVGKEEVPAGSSASTVGTVTSSVAGEPMETSPVIHLVTDAKGTVIHEVHVQMQELPLGMKALTPESADCEELPCSRENSRENLLHQAMQNSGIVLDRVAGEESTLQPAPPSGSSPQSLGDGPSELPLLEVEQIETVASEAATVPRTHPCSQCSETFPSAATLEAHKRGHIGPRPFTCTQCGKAFPKAYLLKKHQEVHVHERRFRCGDCGKLYKTIAHVRGHRRVHSDERPFPCPQCGKRYKTKNAQQVHFRTHLEEKPHVCQFCSRGFREKGSLVRHVRHHTGEKPFKCYKCGRGFAEHGTLNRHLRTKGGCLLEVEELLVSEESPSAAATVLAEDPHTVLVEFSSVVADTQEYIIEATADDTETSEATEIIEGTQTEVDSHIMKVVQQIVHQAGAGHQIIVQNVTMDQEAALGSEAAAADTITIATPESLTEQVAMTLASAISEGTVLTARAGPNSTEQATVTMVSSEDIEILEHGGELVIASPEGQLEVQTVIV

Target ORF information:

RefSeq Version	XM_008767590.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus E4F transcription factor 1 (E4f1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008767590.2

ORF Insert Sequence:

ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCGGGCCGAA 
GCCGGGCATG AAGCGGGCGA GGGCGGGGTT GCAGCGGCGG CGGCCTTGTC CAACGGCGGC 
TTTCTTGGGC TCCCAGCGCC CTTCAGCGAG GAAGATGAGG ATGACGTACA CAGATGTGGA 
CGCTGCCAAG TGGAATTCAC TGCCTTGGAG GACTTTGTCC AGCACAAGAT TCAGAAGACC 
TGCCATCGGG CCCCACAAGA GGCCCTGCCC ACCACCTCTG CTGCCACTTC GTTGCTGGGC 
CAGGAGGTGG TGCCAACAGC AGCAGAGGAA GGTCCAGAGG AACCCATCAC TGTGGCCCAC 
ATCGTGGTGG AGGCCACCTC TCTGGCAGAA GACATGAGCC ATGCTCCTGA CCTTGTTGGA 
AGTGGGCACA TCAAAGAGGT CATTGTGGCT GCTGAGGCAG AGCCAGGGGA TGGCGAGATG 
GCAGAGGCTC CAGGCAGTCC TAACCATCAG GAACTTGGGC TTATTGGGGA GGGCGAGCAG 
GCCCAAGTCA AGCTGCTGGT GAACAAGGAA GGCCGCTACG TGTGCATGCT ATGTCACAAG 
ACCTTCAAAA CGGGCAGCAT CCTCAAGGCT CACATGGTAA CACACAGCAG CCGCAAGGAC 
CACGAGTGCA AGCTCTGTGG GGCCTCCTTT CGGACCAAGG GCTCTCTGAT CCGGCACCAC 
CGACGGCACA CTGATGAGCG TCCCTACAAA TGTGCCAAGT GTGGGAAGAG TTTCCGAGAG 
TCAGGCGCAC TGACTCGGCA CCTCAAATCT CTCACTCCAT GCACAGAAAA GATCCGTTTC 
AGCATAAGCA AGGACACAGC CGTGGGCAAA GAGGAAGTGC CTGCAGGGTC CAGTGCCTCC 
ACTGTGGGGA CAGTGACATC GTCAGTGGCA GGAGAACCCA TGGAGACATC ACCTGTGATT 
CACCTGGTGA CAGATGCCAA GGGCACTGTC ATCCATGAAG TCCATGTCCA GATGCAGGAG 
CTTCCCCTGG GCATGAAAGC CCTGACCCCA GAGTCGGCAG ACTGTGAGGA GCTTCCCTGC 
TCCCGTGAGA ACAGCCGGGA GAACCTGCTA CATCAGGCCA TGCAGAATTC TGGCATCGTC 
CTCGACAGGG TTGCTGGGGA GGAGAGTACC CTGCAGCCAG CCCCTCCTTC TGGGTCCAGT 
CCCCAGTCCC TGGGAGATGG ACCCTCAGAG CTGCCACTGC TGGAGGTGGA GCAGATAGAG 
ACAGTAGCCA GTGAGGCAGC CACTGTGCCC AGGACCCACC CGTGCTCTCA GTGCAGTGAG 
ACTTTCCCAT CAGCGGCTAC CTTGGAGGCC CACAAGAGAG GTCATATAGG GCCGAGGCCA 
TTCACCTGCA CACAGTGTGG CAAGGCCTTC CCCAAAGCCT ACCTGCTCAA GAAGCACCAG 
GAGGTGCATG TGCACGAGCG CCGCTTCCGT TGTGGAGACT GTGGGAAGCT TTATAAGACC 
ATTGCCCATG TGCGGGGCCA CCGGCGTGTC CACTCCGACG AGAGGCCTTT CCCTTGTCCC 
CAGTGTGGCA AGCGTTACAA GACCAAGAAT GCCCAGCAAG TGCACTTCCG GACACATTTG 
GAAGAAAAGC CCCACGTGTG CCAGTTCTGC AGCCGAGGCT TCCGGGAGAA GGGCTCTCTA 
GTGCGGCATG TGAGGCACCA CACCGGCGAG AAACCTTTCA AGTGCTACAA ATGTGGCCGT 
GGCTTTGCAG AGCATGGCAC ACTCAACCGG CACCTGCGCA CTAAAGGGGG CTGCCTGCTC 
GAAGTGGAGG AGTTGCTGGT ATCCGAGGAG AGCCCCTCTG CGGCTGCTAC TGTGCTTGCA 
GAAGACCCCC ACACGGTGCT GGTGGAGTTC TCATCTGTGG TGGCTGATAC CCAAGAGTAC 
ATTATTGAGG CTACTGCGGA TGACACAGAG ACCAGTGAAG CCACTGAGAT CATTGAGGGC 
ACGCAGACAG AGGTGGATAG TCACATCATG AAGGTGGTCC AGCAGATCGT GCACCAGGCT 
GGCGCTGGGC ACCAGATCAT TGTGCAGAAT GTGACCATGG ACCAGGAGGC AGCACTGGGC 
TCAGAGGCAG CTGCCGCGGA CACGATCACC ATTGCCACTC CTGAGAGTCT TACTGAGCAG 
GTGGCCATGA CGCTGGCTTC AGCCATCAGT GAGGGCACTG TGCTCACAGC CCGGGCAGGG 
CCAAACAGTA CTGAACAGGC CACTGTGACA ATGGTGTCAT CAGAGGACAT CGAGATCCTT 
GAGCACGGAG GGGAGCTGGT CATTGCTTCA CCAGAGGGCC AGCTTGAGGT GCAGACAGTC 
ATTGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

E4f1 ( XM_008767591.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008765813.1 Rattus norvegicus E4F transcription factor 1 (E4f1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa32918
Clone ID Related Accession (Same CDS sequence)	XM_008767591.2
Accession Version	XM_008767591.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2241bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	transcription factor E4F1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767591.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGAGGG CTGTGTTGGG TACACTAAGG ATTATTTCTC TAGATGAGGA TGACGTACAC 
AGATGTGGAC GCTGCCAAGT GGAATTCACT GCCTTGGAGG ACTTTGTCCA GCACAAGATT 
CAGAAGACCT GCCATCGGGC CCCACAAGAG GCCCTGCCCA CCACCTCTGC TGCCACTTCG 
TTGCTGGGCC AGGAGGTGGT GCCAACAGCA GCAGAGGAAG GTCCAGAGGA ACCCATCACT 
GTGGCCCACA TCGTGGTGGA GGCCACCTCT CTGGCAGAAG ACATGAGCCA TGCTCCTGAC 
CTTGTTGGAA GTGGGCACAT CAAAGAGGTC ATTGTGGCTG CTGAGGCAGA GCCAGGGGAT 
GGCGAGATGG CAGAGGCTCC AGGCAGTCCT AACCATCAGG AACTTGGGCT TATTGGGGAG 
GGCGAGCAGG CCCAAGTCAA GCTGCTGGTG AACAAGGAAG GCCGCTACGT GTGCATGCTA 
TGTCACAAGA CCTTCAAAAC GGGCAGCATC CTCAAGGCTC ACATGGTAAC ACACAGCAGC 
CGCAAGGACC ACGAGTGCAA GCTCTGTGGG GCCTCCTTTC GGACCAAGGG CTCTCTGATC 
CGGCACCACC GACGGCACAC TGATGAGCGT CCCTACAAAT GTGCCAAGTG TGGGAAGAGT 
TTCCGAGAGT CAGGCGCACT GACTCGGCAC CTCAAATCTC TCACTCCATG CACAGAAAAG 
ATCCGTTTCA GCATAAGCAA GGACACAGCC GTGGGCAAAG AGGAAGTGCC TGCAGGGTCC 
AGTGCCTCCA CTGTGGGGAC AGTGACATCG TCAGTGGCAG GAGAACCCAT GGAGACATCA 
CCTGTGATTC ACCTGGTGAC AGATGCCAAG GGCACTGTCA TCCATGAAGT CCATGTCCAG 
ATGCAGGAGC TTCCCCTGGG CATGAAAGCC CTGACCCCAG AGTCGGCAGA CTGTGAGGAG 
CTTCCCTGCT CCCGTGAGAA CAGCCGGGAG AACCTGCTAC ATCAGGCCAT GCAGAATTCT 
GGCATCGTCC TCGACAGGGT TGCTGGGGAG GAGAGTACCC TGCAGCCAGC CCCTCCTTCT 
GGGTCCAGTC CCCAGTCCCT GGGAGATGGA CCCTCAGAGC TGCCACTGCT GGAGGTGGAG 
CAGATAGAGA CACAGGTAGC CAGTGAGGCA GCCACTGTGC CCAGGACCCA CCCGTGCTCT 
CAGTGCAGTG AGACTTTCCC ATCAGCGGCT ACCTTGGAGG CCCACAAGAG AGGTCATATA 
GGGCCGAGGC CATTCACCTG CACACAGTGT GGCAAGGCCT TCCCCAAAGC CTACCTGCTC 
AAGAAGCACC AGGAGGTGCA TGTGCACGAG CGCCGCTTCC GTTGTGGAGA CTGTGGGAAG 
CTTTATAAGA CCATTGCCCA TGTGCGGGGC CACCGGCGTG TCCACTCCGA CGAGAGGCCT 
TTCCCTTGTC CCCAGTGTGG CAAGCGTTAC AAGACCAAGA ATGCCCAGCA AGTGCACTTC 
CGGACACATT TGGAAGAAAA GCCCCACGTG TGCCAGTTCT GCAGCCGAGG CTTCCGGGAG 
AAGGGCTCTC TAGTGCGGCA TGTGAGGCAC CACACCGGCG AGAAACCTTT CAAGTGCTAC 
AAATGTGGCC GTGGCTTTGC AGAGCATGGC ACACTCAACC GGCACCTGCG CACTAAAGGG 
GGCTGCCTGC TCGAAGTGGA GGAGTTGCTG GTATCCGAGG AGAGCCCCTC TGCGGCTGCT 
ACTGTGCTTG CAGAAGACCC CCACACGGTG CTGGTGGAGT TCTCATCTGT GGTGGCTGAT 
ACCCAAGAGT ACATTATTGA GGCTACTGCG GATGACACAG AGACCAGTGA AGCCACTGAG 
ATCATTGAGG GCACGCAGAC AGAGGTGGAT AGTCACATCA TGAAGGTGGT CCAGCAGATC 
GTGCACCAGG CTGGCGCTGG GCACCAGATC ATTGTGCAGA ATGTGACCAT GGACCAGGAG 
GCAGCACTGG GCTCAGAGGC AGCTGCCGCG GACACGATCA CCATTGCCAC TCCTGAGAGT 
CTTACTGAGC AGGTGGCCAT GACGCTGGCT TCAGCCATCA GTGAGGGCAC TGTGCTCACA 
GCCCGGGCAG GGCCAAACAG TACTGAACAG GCCACTGTGA CAATGGTGTC ATCAGAGGAC 
ATCGAGATCC TTGAGCACGG AGGGGAGCTG GTCATTGCTT CACCAGAGGG CCAGCTTGAG 
GTGCAGACAG TCATTGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008765813.1
CDS	749..2989
Translation	MPRAVLGTLRIISLDEDDVHRCGRCQVEFTALEDFVQHKIQKTCHRAPQEALPTTSAATSLLGQEVVPTAAEEGPEEPITVAHIVVEATSLAEDMSHAPDLVGSGHIKEVIVAAEAEPGDGEMAEAPGSPNHQELGLIGEGEQAQVKLLVNKEGRYVCMLCHKTFKTGSILKAHMVTHSSRKDHECKLCGASFRTKGSLIRHHRRHTDERPYKCAKCGKSFRESGALTRHLKSLTPCTEKIRFSISKDTAVGKEEVPAGSSASTVGTVTSSVAGEPMETSPVIHLVTDAKGTVIHEVHVQMQELPLGMKALTPESADCEELPCSRENSRENLLHQAMQNSGIVLDRVAGEESTLQPAPPSGSSPQSLGDGPSELPLLEVEQIETQVASEAATVPRTHPCSQCSETFPSAATLEAHKRGHIGPRPFTCTQCGKAFPKAYLLKKHQEVHVHERRFRCGDCGKLYKTIAHVRGHRRVHSDERPFPCPQCGKRYKTKNAQQVHFRTHLEEKPHVCQFCSRGFREKGSLVRHVRHHTGEKPFKCYKCGRGFAEHGTLNRHLRTKGGCLLEVEELLVSEESPSAAATVLAEDPHTVLVEFSSVVADTQEYIIEATADDTETSEATEIIEGTQTEVDSHIMKVVQQIVHQAGAGHQIIVQNVTMDQEAALGSEAAAADTITIATPESLTEQVAMTLASAISEGTVLTARAGPNSTEQATVTMVSSEDIEILEHGGELVIASPEGQLEVQTVIV

Target ORF information:

RefSeq Version	XM_008767591.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus E4F transcription factor 1 (E4f1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008767591.2

ORF Insert Sequence:

ATGCCGAGGG CTGTGTTGGG TACACTAAGG ATTATTTCTC TAGATGAGGA TGACGTACAC 
AGATGTGGAC GCTGCCAAGT GGAATTCACT GCCTTGGAGG ACTTTGTCCA GCACAAGATT 
CAGAAGACCT GCCATCGGGC CCCACAAGAG GCCCTGCCCA CCACCTCTGC TGCCACTTCG 
TTGCTGGGCC AGGAGGTGGT GCCAACAGCA GCAGAGGAAG GTCCAGAGGA ACCCATCACT 
GTGGCCCACA TCGTGGTGGA GGCCACCTCT CTGGCAGAAG ACATGAGCCA TGCTCCTGAC 
CTTGTTGGAA GTGGGCACAT CAAAGAGGTC ATTGTGGCTG CTGAGGCAGA GCCAGGGGAT 
GGCGAGATGG CAGAGGCTCC AGGCAGTCCT AACCATCAGG AACTTGGGCT TATTGGGGAG 
GGCGAGCAGG CCCAAGTCAA GCTGCTGGTG AACAAGGAAG GCCGCTACGT GTGCATGCTA 
TGTCACAAGA CCTTCAAAAC GGGCAGCATC CTCAAGGCTC ACATGGTAAC ACACAGCAGC 
CGCAAGGACC ACGAGTGCAA GCTCTGTGGG GCCTCCTTTC GGACCAAGGG CTCTCTGATC 
CGGCACCACC GACGGCACAC TGATGAGCGT CCCTACAAAT GTGCCAAGTG TGGGAAGAGT 
TTCCGAGAGT CAGGCGCACT GACTCGGCAC CTCAAATCTC TCACTCCATG CACAGAAAAG 
ATCCGTTTCA GCATAAGCAA GGACACAGCC GTGGGCAAAG AGGAAGTGCC TGCAGGGTCC 
AGTGCCTCCA CTGTGGGGAC AGTGACATCG TCAGTGGCAG GAGAACCCAT GGAGACATCA 
CCTGTGATTC ACCTGGTGAC AGATGCCAAG GGCACTGTCA TCCATGAAGT CCATGTCCAG 
ATGCAGGAGC TTCCCCTGGG CATGAAAGCC CTGACCCCAG AGTCGGCAGA CTGTGAGGAG 
CTTCCCTGCT CCCGTGAGAA CAGCCGGGAG AACCTGCTAC ATCAGGCCAT GCAGAATTCT 
GGCATCGTCC TCGACAGGGT TGCTGGGGAG GAGAGTACCC TGCAGCCAGC CCCTCCTTCT 
GGGTCCAGTC CCCAGTCCCT GGGAGATGGA CCCTCAGAGC TGCCACTGCT GGAGGTGGAG 
CAGATAGAGA CACAGGTAGC CAGTGAGGCA GCCACTGTGC CCAGGACCCA CCCGTGCTCT 
CAGTGCAGTG AGACTTTCCC ATCAGCGGCT ACCTTGGAGG CCCACAAGAG AGGTCATATA 
GGGCCGAGGC CATTCACCTG CACACAGTGT GGCAAGGCCT TCCCCAAAGC CTACCTGCTC 
AAGAAGCACC AGGAGGTGCA TGTGCACGAG CGCCGCTTCC GTTGTGGAGA CTGTGGGAAG 
CTTTATAAGA CCATTGCCCA TGTGCGGGGC CACCGGCGTG TCCACTCCGA CGAGAGGCCT 
TTCCCTTGTC CCCAGTGTGG CAAGCGTTAC AAGACCAAGA ATGCCCAGCA AGTGCACTTC 
CGGACACATT TGGAAGAAAA GCCCCACGTG TGCCAGTTCT GCAGCCGAGG CTTCCGGGAG 
AAGGGCTCTC TAGTGCGGCA TGTGAGGCAC CACACCGGCG AGAAACCTTT CAAGTGCTAC 
AAATGTGGCC GTGGCTTTGC AGAGCATGGC ACACTCAACC GGCACCTGCG CACTAAAGGG 
GGCTGCCTGC TCGAAGTGGA GGAGTTGCTG GTATCCGAGG AGAGCCCCTC TGCGGCTGCT 
ACTGTGCTTG CAGAAGACCC CCACACGGTG CTGGTGGAGT TCTCATCTGT GGTGGCTGAT 
ACCCAAGAGT ACATTATTGA GGCTACTGCG GATGACACAG AGACCAGTGA AGCCACTGAG 
ATCATTGAGG GCACGCAGAC AGAGGTGGAT AGTCACATCA TGAAGGTGGT CCAGCAGATC 
GTGCACCAGG CTGGCGCTGG GCACCAGATC ATTGTGCAGA ATGTGACCAT GGACCAGGAG 
GCAGCACTGG GCTCAGAGGC AGCTGCCGCG GACACGATCA CCATTGCCAC TCCTGAGAGT 
CTTACTGAGC AGGTGGCCAT GACGCTGGCT TCAGCCATCA GTGAGGGCAC TGTGCTCACA 
GCCCGGGCAG GGCCAAACAG TACTGAACAG GCCACTGTGA CAATGGTGTC ATCAGAGGAC 
ATCGAGATCC TTGAGCACGG AGGGGAGCTG GTCATTGCTT CACCAGAGGG CCAGCTTGAG 
GTGCAGACAG TCATTGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

E4f1 ( XM_017597525.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017453014.1 Rattus norvegicus E4F transcription factor 1 (E4f1), transcript variant X4, mRNA.

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CloneID	ORa57474
Clone ID Related Accession (Same CDS sequence)	XM_017597525.1
Accession Version	XM_017597525.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	transcription factor E4F1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCGGGCCGAA 
GCCGGGCATG AAGCGGGCGA GGGCGGGGTT GCAGCGGCGG CGGCCTTGTC CAACGGCGGC 
TTTCTTGGGC TCCCAGCGCC CTTCAGCGAG GAAGATGAGG ATGACGTACA CAGATGTGGA 
CGCTGCCAAG TGGAATTCAC TGCCTTGGAG GACTTTGTCC AGCACAAGAT TCAGAAGACC 
TGCCATCGGG CCCCACAAGA GGCCCTGCCC ACCACCTCTG CTGCCACTTC GTTGCTGGGC 
CAGGAGGTGG TGCCAACAGC AGCAGAGGAA GGTCCAGAGG AACCCATCAC TGTGGCCCAC 
ATCGTGGTGG AGGCCACCTC TCTGGCAGAA GACATGAGCC ATGCTCCTGA CCTTGTTGGA 
AGTGGGCACA TCAAAGAGGT CATTGTGGCT GCTGAGGCAG AGCCAGGGGA TGGCGAGATG 
GCAGAGGCTC CAGGCAGTCC TAACCATCAG GAACTTGGGC TTATTGGGGA GGGCGAGCAG 
GCCCAAGTCA AGCTGCTGGT GAACAAGGAA GGCCGCTACG TGTGCATGCT ATGTCACAAG 
ACCTTCAAAA CGGGCAGCAT CCTCAAGGCT CACATGGTAA CACACAGCAG CCGCAAGGAC 
CACGAGTGCA AGCTCTGTGG GGCCTCCTTT CGGACCAAGG GCTCTCTGAT CCGGCACCAC 
CGACGGCACA CTGAAAAGAT CCGTTTCAGC ATAAGCAAGG ACACAGCCGT GGGCAAAGAG 
GAAGTGCCTG CAGGGTCCAG TGCCTCCACT GTGGGGACAG TGACATCGTC AGTGGCAGGA 
GAACCCATGG AGACATCACC TGTGATTCAC CTGGTGACAG ATGCCAAGGG CACTGTCATC 
CATGAAGTCC ATGTCCAGAT GCAGGAGCTT CCCCTGGGCA TGAAAGCCCT GACCCCAGAG 
TCGGCAGACT GTGAGGAGCT TCCCTGCTCC CGTGAGAACA GCCGGGAGAA CCTGCTACAT 
CAGGCCATGC AGAATTCTGG CATCGTCCTC GACAGGGTTG CTGGGGAGGA GAGTACCCTG 
CAGCCAGCCC CTCCTTCTGG GTCCAGTCCC CAGTCCCTGG GAGATGGACC CTCAGAGCTG 
CCACTGCTGG AGGTGGAGCA GATAGAGACA CAGGTAGCCA GTGAGGCAGC CACTGTGCCC 
AGGACCCACC CGTGCTCTCA GTGCAGTGAG ACTTTCCCAT CAGCGGCTAC CTTGGAGGCC 
CACAAGAGAG GTCATATAGG GCCGAGGCCA TTCACCTGCA CACAGTGTGG CAAGGCCTTC 
CCCAAAGCCT ACCTGCTCAA GAAGCACCAG GAGGTGCATG TGCACGAGCG CCGCTTCCGT 
TGTGGAGACT GTGGGAAGCT TTATAAGACC ATTGCCCATG TGCGGGGCCA CCGGCGTGTC 
CACTCCGACG AGAGGCCTTT CCCTTGTCCC CAGTGTGGCA AGCGTTACAA GACCAAGAAT 
GCCCAGCAAG TGCACTTCCG GACACATTTG GAAGAAAAGC CCCACGTGTG CCAGTTCTGC 
AGCCGAGGCT TCCGGGAGAA GGGCTCTCTA GTGCGGCATG TGAGGCACCA CACCGGCGAG 
AAACCTTTCA AGTGCTACAA ATGTGGCCGT GGCTTTGCAG AGCATGGCAC ACTCAACCGG 
CACCTGCGCA CTAAAGGGGG CTGCCTGCTC GAAGTGGAGG AGTTGCTGGT ATCCGAGGAG 
AGCCCCTCTG CGGCTGCTAC TGTGCTTGCA GAAGACCCCC ACACGGTGCT GGTGGAGTTC 
TCATCTGTGG TGGCTGATAC CCAAGAGTAC ATTATTGAGG CTACTGCGGA TGACACAGAG 
ACCAGTGAAG CCACTGAGAT CATTGAGGGC ACGCAGACAG AGGTGGATAG TCACATCATG 
AAGGTGGTCC AGCAGATCGT GCACCAGGCT GGCGCTGGGC ACCAGATCAT TGTGCAGAAT 
GTGACCATGG ACCAGGAGGC AGCACTGGGC TCAGAGGCAG CTGCCGCGGA CACGATCACC 
ATTGCCACTC CTGAGAGTCT TACTGAGCAG GTGGCCATGA CGCTGGCTTC AGCCATCAGT 
GAGGGCACTG TGCTCACAGC CCGGGCAGGG CCAAACAGTA CTGAACAGGC CACTGTGACA 
ATGGTGTCAT CAGAGGACAT CGAGATCCTT GAGCACGGAG GGGAGCTGGT CATTGCTTCA 
CCAGAGGGCC AGCTTGAGGT GCAGACAGTC ATTGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017453014.1
CDS	1135..3393
Translation	MEGAMAVRVTAAHTAEARAEAGHEAGEGGVAAAAALSNGGFLGLPAPFSEEDEDDVHRCGRCQVEFTALEDFVQHKIQKTCHRAPQEALPTTSAATSLLGQEVVPTAAEEGPEEPITVAHIVVEATSLAEDMSHAPDLVGSGHIKEVIVAAEAEPGDGEMAEAPGSPNHQELGLIGEGEQAQVKLLVNKEGRYVCMLCHKTFKTGSILKAHMVTHSSRKDHECKLCGASFRTKGSLIRHHRRHTEKIRFSISKDTAVGKEEVPAGSSASTVGTVTSSVAGEPMETSPVIHLVTDAKGTVIHEVHVQMQELPLGMKALTPESADCEELPCSRENSRENLLHQAMQNSGIVLDRVAGEESTLQPAPPSGSSPQSLGDGPSELPLLEVEQIETQVASEAATVPRTHPCSQCSETFPSAATLEAHKRGHIGPRPFTCTQCGKAFPKAYLLKKHQEVHVHERRFRCGDCGKLYKTIAHVRGHRRVHSDERPFPCPQCGKRYKTKNAQQVHFRTHLEEKPHVCQFCSRGFREKGSLVRHVRHHTGEKPFKCYKCGRGFAEHGTLNRHLRTKGGCLLEVEELLVSEESPSAAATVLAEDPHTVLVEFSSVVADTQEYIIEATADDTETSEATEIIEGTQTEVDSHIMKVVQQIVHQAGAGHQIIVQNVTMDQEAALGSEAAAADTITIATPESLTEQVAMTLASAISEGTVLTARAGPNSTEQATVTMVSSEDIEILEHGGELVIASPEGQLEVQTVIV

Target ORF information:

RefSeq Version	XM_017597525.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus E4F transcription factor 1 (E4f1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017597525.1

ORF Insert Sequence:

ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCTCATA CGGCAGAAGC CCGGGCCGAA 
GCCGGGCATG AAGCGGGCGA GGGCGGGGTT GCAGCGGCGG CGGCCTTGTC CAACGGCGGC 
TTTCTTGGGC TCCCAGCGCC CTTCAGCGAG GAAGATGAGG ATGACGTACA CAGATGTGGA 
CGCTGCCAAG TGGAATTCAC TGCCTTGGAG GACTTTGTCC AGCACAAGAT TCAGAAGACC 
TGCCATCGGG CCCCACAAGA GGCCCTGCCC ACCACCTCTG CTGCCACTTC GTTGCTGGGC 
CAGGAGGTGG TGCCAACAGC AGCAGAGGAA GGTCCAGAGG AACCCATCAC TGTGGCCCAC 
ATCGTGGTGG AGGCCACCTC TCTGGCAGAA GACATGAGCC ATGCTCCTGA CCTTGTTGGA 
AGTGGGCACA TCAAAGAGGT CATTGTGGCT GCTGAGGCAG AGCCAGGGGA TGGCGAGATG 
GCAGAGGCTC CAGGCAGTCC TAACCATCAG GAACTTGGGC TTATTGGGGA GGGCGAGCAG 
GCCCAAGTCA AGCTGCTGGT GAACAAGGAA GGCCGCTACG TGTGCATGCT ATGTCACAAG 
ACCTTCAAAA CGGGCAGCAT CCTCAAGGCT CACATGGTAA CACACAGCAG CCGCAAGGAC 
CACGAGTGCA AGCTCTGTGG GGCCTCCTTT CGGACCAAGG GCTCTCTGAT CCGGCACCAC 
CGACGGCACA CTGAAAAGAT CCGTTTCAGC ATAAGCAAGG ACACAGCCGT GGGCAAAGAG 
GAAGTGCCTG CAGGGTCCAG TGCCTCCACT GTGGGGACAG TGACATCGTC AGTGGCAGGA 
GAACCCATGG AGACATCACC TGTGATTCAC CTGGTGACAG ATGCCAAGGG CACTGTCATC 
CATGAAGTCC ATGTCCAGAT GCAGGAGCTT CCCCTGGGCA TGAAAGCCCT GACCCCAGAG 
TCGGCAGACT GTGAGGAGCT TCCCTGCTCC CGTGAGAACA GCCGGGAGAA CCTGCTACAT 
CAGGCCATGC AGAATTCTGG CATCGTCCTC GACAGGGTTG CTGGGGAGGA GAGTACCCTG 
CAGCCAGCCC CTCCTTCTGG GTCCAGTCCC CAGTCCCTGG GAGATGGACC CTCAGAGCTG 
CCACTGCTGG AGGTGGAGCA GATAGAGACA CAGGTAGCCA GTGAGGCAGC CACTGTGCCC 
AGGACCCACC CGTGCTCTCA GTGCAGTGAG ACTTTCCCAT CAGCGGCTAC CTTGGAGGCC 
CACAAGAGAG GTCATATAGG GCCGAGGCCA TTCACCTGCA CACAGTGTGG CAAGGCCTTC 
CCCAAAGCCT ACCTGCTCAA GAAGCACCAG GAGGTGCATG TGCACGAGCG CCGCTTCCGT 
TGTGGAGACT GTGGGAAGCT TTATAAGACC ATTGCCCATG TGCGGGGCCA CCGGCGTGTC 
CACTCCGACG AGAGGCCTTT CCCTTGTCCC CAGTGTGGCA AGCGTTACAA GACCAAGAAT 
GCCCAGCAAG TGCACTTCCG GACACATTTG GAAGAAAAGC CCCACGTGTG CCAGTTCTGC 
AGCCGAGGCT TCCGGGAGAA GGGCTCTCTA GTGCGGCATG TGAGGCACCA CACCGGCGAG 
AAACCTTTCA AGTGCTACAA ATGTGGCCGT GGCTTTGCAG AGCATGGCAC ACTCAACCGG 
CACCTGCGCA CTAAAGGGGG CTGCCTGCTC GAAGTGGAGG AGTTGCTGGT ATCCGAGGAG 
AGCCCCTCTG CGGCTGCTAC TGTGCTTGCA GAAGACCCCC ACACGGTGCT GGTGGAGTTC 
TCATCTGTGG TGGCTGATAC CCAAGAGTAC ATTATTGAGG CTACTGCGGA TGACACAGAG 
ACCAGTGAAG CCACTGAGAT CATTGAGGGC ACGCAGACAG AGGTGGATAG TCACATCATG 
AAGGTGGTCC AGCAGATCGT GCACCAGGCT GGCGCTGGGC ACCAGATCAT TGTGCAGAAT 
GTGACCATGG ACCAGGAGGC AGCACTGGGC TCAGAGGCAG CTGCCGCGGA CACGATCACC 
ATTGCCACTC CTGAGAGTCT TACTGAGCAG GTGGCCATGA CGCTGGCTTC AGCCATCAGT 
GAGGGCACTG TGCTCACAGC CCGGGCAGGG CCAAACAGTA CTGAACAGGC CACTGTGACA 
ATGGTGTCAT CAGAGGACAT CGAGATCCTT GAGCACGGAG GGGAGCTGGT CATTGCTTCA 
CCAGAGGGCC AGCTTGAGGT GCAGACAGTC ATTGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

E4f1 ( XM_017597526.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017453015.1 Rattus norvegicus E4F transcription factor 1 (E4f1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ORa57475
Clone ID Related Accession (Same CDS sequence)	XM_017597526.1
Accession Version	XM_017597526.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	transcription factor E4F1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCATG CTCCTGACCT TGTTGGAAGT GGGCACATCA AAGAGGTCAT TGTGGCTGCT 
GAGGCAGAGC CAGGGGATGG CGAGATGGCA GAGGCTCCAG GCAGTCCTAA CCATCAGGAA 
CTTGGGCTTA TTGGGGAGGG CGAGCAGGCC CAAGTCAAGC TGCTGGTGAA CAAGGAAGGC 
CGCTACGTGT GCATGCTATG TCACAAGACC TTCAAAACGG GCAGCATCCT CAAGGCTCAC 
ATGGTAACAC ACAGCAGCCG CAAGGACCAC GAGTGCAAGC TCTGTGGGGC CTCCTTTCGG 
ACCAAGGGCT CTCTGATCCG GCACCACCGA CGGCACACTG ATGAGCGTCC CTACAAATGT 
GCCAAGTGTG GGAAGAGTTT CCGAGAGTCA GGCGCACTGA CTCGGCACCT CAAATCTCTC 
ACTCCATGCA CAGAAAAGAT CCGTTTCAGC ATAAGCAAGG ACACAGCCGT GGGCAAAGAG 
GAAGTGCCTG CAGGGTCCAG TGCCTCCACT GTGGGGACAG TGACATCGTC AGTGGCAGGA 
GAACCCATGG AGACATCACC TGTGATTCAC CTGGTGACAG ATGCCAAGGG CACTGTCATC 
CATGAAGTCC ATGTCCAGAT GCAGGAGCTT CCCCTGGGCA TGAAAGCCCT GACCCCAGAG 
TCGGCAGACT GTGAGGAGCT TCCCTGCTCC CGTGAGAACA GCCGGGAGAA CCTGCTACAT 
CAGGCCATGC AGAATTCTGG CATCGTCCTC GACAGGGTTG CTGGGGAGGA GAGTACCCTG 
CAGCCAGCCC CTCCTTCTGG GTCCAGTCCC CAGTCCCTGG GAGATGGACC CTCAGAGCTG 
CCACTGCTGG AGGTGGAGCA GATAGAGACA CAGGTAGCCA GTGAGGCAGC CACTGTGCCC 
AGGACCCACC CGTGCTCTCA GTGCAGTGAG ACTTTCCCAT CAGCGGCTAC CTTGGAGGCC 
CACAAGAGAG GTCATATAGG GCCGAGGCCA TTCACCTGCA CACAGTGTGG CAAGGCCTTC 
CCCAAAGCCT ACCTGCTCAA GAAGCACCAG GAGGTGCATG TGCACGAGCG CCGCTTCCGT 
TGTGGAGACT GTGGGAAGCT TTATAAGACC ATTGCCCATG TGCGGGGCCA CCGGCGTGTC 
CACTCCGACG AGAGGCCTTT CCCTTGTCCC CAGTGTGGCA AGCGTTACAA GACCAAGAAT 
GCCCAGCAAG TGCACTTCCG GACACATTTG GAAGAAAAGC CCCACGTGTG CCAGTTCTGC 
AGCCGAGGCT TCCGGGAGAA GGGCTCTCTA GTGCGGCATG TGAGGCACCA CACCGGCGAG 
AAACCTTTCA AGTGCTACAA ATGTGGCCGT GGCTTTGCAG AGCATGGCAC ACTCAACCGG 
CACCTGCGCA CTAAAGGGGG CTGCCTGCTC GAAGTGGAGG AGTTGCTGGT ATCCGAGGAG 
AGCCCCTCTG CGGCTGCTAC TGTGCTTGCA GAAGACCCCC ACACGGTGCT GGTGGAGTTC 
TCATCTGTGG TGGCTGATAC CCAAGAGTAC ATTATTGAGG CTACTGCGGA TGACACAGAG 
ACCAGTGAAG CCACTGAGAT CATTGAGGGC ACGCAGACAG AGGTGGATAG TCACATCATG 
AAGGTGGTCC AGCAGATCGT GCACCAGGCT GGCGCTGGGC ACCAGATCAT TGTGCAGAAT 
GTGACCATGG ACCAGGAGGC AGCACTGGGC TCAGAGGCAG CTGCCGCGGA CACGATCACC 
ATTGCCACTC CTGAGAGTCT TACTGAGCAG GTGGCCATGA CGCTGGCTTC AGCCATCAGT 
GAGGGCACTG TGCTCACAGC CCGGGCAGGG CCAAACAGTA CTGAACAGGC CACTGTGACA 
ATGGTGTCAT CAGAGGACAT CGAGATCCTT GAGCACGGAG GGGAGCTGGT CATTGCTTCA 
CCAGAGGGCC AGCTTGAGGT GCAGACAGTC ATTGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017453015.1
CDS	1201..3159
Translation	MSHAPDLVGSGHIKEVIVAAEAEPGDGEMAEAPGSPNHQELGLIGEGEQAQVKLLVNKEGRYVCMLCHKTFKTGSILKAHMVTHSSRKDHECKLCGASFRTKGSLIRHHRRHTDERPYKCAKCGKSFRESGALTRHLKSLTPCTEKIRFSISKDTAVGKEEVPAGSSASTVGTVTSSVAGEPMETSPVIHLVTDAKGTVIHEVHVQMQELPLGMKALTPESADCEELPCSRENSRENLLHQAMQNSGIVLDRVAGEESTLQPAPPSGSSPQSLGDGPSELPLLEVEQIETQVASEAATVPRTHPCSQCSETFPSAATLEAHKRGHIGPRPFTCTQCGKAFPKAYLLKKHQEVHVHERRFRCGDCGKLYKTIAHVRGHRRVHSDERPFPCPQCGKRYKTKNAQQVHFRTHLEEKPHVCQFCSRGFREKGSLVRHVRHHTGEKPFKCYKCGRGFAEHGTLNRHLRTKGGCLLEVEELLVSEESPSAAATVLAEDPHTVLVEFSSVVADTQEYIIEATADDTETSEATEIIEGTQTEVDSHIMKVVQQIVHQAGAGHQIIVQNVTMDQEAALGSEAAAADTITIATPESLTEQVAMTLASAISEGTVLTARAGPNSTEQATVTMVSSEDIEILEHGGELVIASPEGQLEVQTVIV

Target ORF information:

RefSeq Version	XM_017597526.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus E4F transcription factor 1 (E4f1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017597526.1

ORF Insert Sequence:

ATGAGCCATG CTCCTGACCT TGTTGGAAGT GGGCACATCA AAGAGGTCAT TGTGGCTGCT 
GAGGCAGAGC CAGGGGATGG CGAGATGGCA GAGGCTCCAG GCAGTCCTAA CCATCAGGAA 
CTTGGGCTTA TTGGGGAGGG CGAGCAGGCC CAAGTCAAGC TGCTGGTGAA CAAGGAAGGC 
CGCTACGTGT GCATGCTATG TCACAAGACC TTCAAAACGG GCAGCATCCT CAAGGCTCAC 
ATGGTAACAC ACAGCAGCCG CAAGGACCAC GAGTGCAAGC TCTGTGGGGC CTCCTTTCGG 
ACCAAGGGCT CTCTGATCCG GCACCACCGA CGGCACACTG ATGAGCGTCC CTACAAATGT 
GCCAAGTGTG GGAAGAGTTT CCGAGAGTCA GGCGCACTGA CTCGGCACCT CAAATCTCTC 
ACTCCATGCA CAGAAAAGAT CCGTTTCAGC ATAAGCAAGG ACACAGCCGT GGGCAAAGAG 
GAAGTGCCTG CAGGGTCCAG TGCCTCCACT GTGGGGACAG TGACATCGTC AGTGGCAGGA 
GAACCCATGG AGACATCACC TGTGATTCAC CTGGTGACAG ATGCCAAGGG CACTGTCATC 
CATGAAGTCC ATGTCCAGAT GCAGGAGCTT CCCCTGGGCA TGAAAGCCCT GACCCCAGAG 
TCGGCAGACT GTGAGGAGCT TCCCTGCTCC CGTGAGAACA GCCGGGAGAA CCTGCTACAT 
CAGGCCATGC AGAATTCTGG CATCGTCCTC GACAGGGTTG CTGGGGAGGA GAGTACCCTG 
CAGCCAGCCC CTCCTTCTGG GTCCAGTCCC CAGTCCCTGG GAGATGGACC CTCAGAGCTG 
CCACTGCTGG AGGTGGAGCA GATAGAGACA CAGGTAGCCA GTGAGGCAGC CACTGTGCCC 
AGGACCCACC CGTGCTCTCA GTGCAGTGAG ACTTTCCCAT CAGCGGCTAC CTTGGAGGCC 
CACAAGAGAG GTCATATAGG GCCGAGGCCA TTCACCTGCA CACAGTGTGG CAAGGCCTTC 
CCCAAAGCCT ACCTGCTCAA GAAGCACCAG GAGGTGCATG TGCACGAGCG CCGCTTCCGT 
TGTGGAGACT GTGGGAAGCT TTATAAGACC ATTGCCCATG TGCGGGGCCA CCGGCGTGTC 
CACTCCGACG AGAGGCCTTT CCCTTGTCCC CAGTGTGGCA AGCGTTACAA GACCAAGAAT 
GCCCAGCAAG TGCACTTCCG GACACATTTG GAAGAAAAGC CCCACGTGTG CCAGTTCTGC 
AGCCGAGGCT TCCGGGAGAA GGGCTCTCTA GTGCGGCATG TGAGGCACCA CACCGGCGAG 
AAACCTTTCA AGTGCTACAA ATGTGGCCGT GGCTTTGCAG AGCATGGCAC ACTCAACCGG 
CACCTGCGCA CTAAAGGGGG CTGCCTGCTC GAAGTGGAGG AGTTGCTGGT ATCCGAGGAG 
AGCCCCTCTG CGGCTGCTAC TGTGCTTGCA GAAGACCCCC ACACGGTGCT GGTGGAGTTC 
TCATCTGTGG TGGCTGATAC CCAAGAGTAC ATTATTGAGG CTACTGCGGA TGACACAGAG 
ACCAGTGAAG CCACTGAGAT CATTGAGGGC ACGCAGACAG AGGTGGATAG TCACATCATG 
AAGGTGGTCC AGCAGATCGT GCACCAGGCT GGCGCTGGGC ACCAGATCAT TGTGCAGAAT 
GTGACCATGG ACCAGGAGGC AGCACTGGGC TCAGAGGCAG CTGCCGCGGA CACGATCACC 
ATTGCCACTC CTGAGAGTCT TACTGAGCAG GTGGCCATGA CGCTGGCTTC AGCCATCAGT 
GAGGGCACTG TGCTCACAGC CCGGGCAGGG CCAAACAGTA CTGAACAGGC CACTGTGACA 
ATGGTGTCAT CAGAGGACAT CGAGATCCTT GAGCACGGAG GGGAGCTGGT CATTGCTTCA 
CCAGAGGGCC AGCTTGAGGT GCAGACAGTC ATTGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The LIM-only protein FHL2 is a negative regulator of E4F1.
Oncogene25(40)5475-84(2006 Sep)
Paul C,Lacroix M,Iankova I,Julien E,Sch?fer BW,Labalette C,Wei Y,Le Cam A,Le Cam L,Sardet C

The E4F protein is required for mitotic progression during embryonic cell cycles.
Molecular and cellular biology24(14)6467-75(2004 Jul)
Le Cam L,Lacroix M,Ciemerych MA,Sardet C,Sicinski P

Transcriptional activation of the cyclin A gene by the architectural transcription factor HMGA2.
Molecular and cellular biology23(24)9104-16(2003 Dec)
Tessari MA,Gostissa M,Altamura S,Sgarra R,Rustighi A,Salvagno C,Caretti G,Imbriano C,Mantovani R,Del Sal G,Giancotti V,Manfioletti G

E4F and ATF, two transcription factors that recognize the same site, can be distinguished both physically and functionally: a role for E4F in E1A trans activation.
Molecular and cellular biology10(10)5138-49(1990 Oct)
Rooney RJ,Raychaudhuri P,Nevins JR

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E4f1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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