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SMT3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene Symbol SMT3
Entrez Gene ID 852122
Full Name SUMO family protein SMT3
Gene Type protein-coding
Organism Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA Protein Name
NM_001180818.1 NP_010798.1 SUMO family protein SMT3



Kluyveromyces lactis KLLA0C01628g XP_452268.1
Neurospora crassa NCU09813 XP_964073.1
Saccharomyces cerevisiae (baker's yeast) SMT3 NP_010798.1
Eremothecium gossypii AGOS_AFR697C NP_986245.1
Magnaporthe oryzae (rice blast fungus) MGG_05737 XP_003710648.1


Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Jacq C, Alt-Mörbe J, Andre B, Arnold W, Bahr A, Ballesta JP, Bargues M, Baron L, Becker A, Biteau N, Blöcker H, Blugeon C, Boskovic J, Brandt P, Brückner M, Buitrago MJ, Coster F, Delaveau T, del Rey F, Dujon B, Eide LG, Garcia-Cantalejo JM, Goffeau A, Gomez-Peris A, Zaccaria P
Nature387(6632 Suppl)75-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the library of Smt3 mutants represents a valuable resource for further exploring the functions of sumoylation in cellular stress response and other SUMO-dependent pathways.
Title: A high throughput mutagenic analysis of yeast sumo structure and function.

Ulp2 controls the dynamic range of SUMO chain lengths by trimming them from the distal ends
Title: In Vitro Studies Reveal a Sequential Mode of Chain Processing by the Yeast SUMO (Small Ubiquitin-related Modifier)-specific Protease Ulp2.

Cdk1 and SUMO (Smt3) regulate Swe1 stability
Title: Cdk1 and SUMO regulate Swe1 stability.

the Zn(2+) motif of E1 has a role in SUMO adenylation
Title: Role of the Zn(2+) motif of E1 in SUMO adenylation.

analysis of a SUMO-binding-motif mimic bound to Smt3p-Ubc9p
Title: Structure of a SUMO-binding-motif mimic bound to Smt3p-Ubc9p: conservation of a non-covalent ubiquitin-like protein-E2 complex as a platform for selective interactions within a SUMO pathway.

The following SMT3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMT3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OSi01576 NM_001180818.1
Latest version!
Saccharomyces cerevisiae S288c SUMO family protein SMT3 (SMT3), partial mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $104.30
$149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OSi01576
Clone ID Related Accession (Same CDS sequence) NM_001180818.1
Accession Version NM_001180818.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 306bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-03-15
Organism Saccharomyces cerevisiae S288C
Product SUMO family protein SMT3
Comment REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001136). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

1
61
121
181
241
301
ATGTCGGACT CAGAAGTCAA TCAAGAAGCT AAGCCAGAGG TCAAGCCAGA AGTCAAGCCT 
GAGACTCACA TCAATTTAAA GGTGTCCGAT GGATCTTCAG AGATCTTCTT CAAGATCAAA
AAGACCACTC CTTTAAGAAG GCTGATGGAA GCGTTCGCTA AAAGACAGGG TAAGGAAATG
GACTCCTTAA GATTCTTGTA CGACGGTATT AGAATTCAAG CTGATCAGAC CCCTGAAGAT
TTGGACATGG AGGATAACGA TATTATTGAG GCTCACAGAG AACAGATTGG TGGTGCTACG
TATTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_010798.1
CDS1..306
Misc Feature(1)1..303
Misc Feature(2)order(52..57,73..75,79..81,139..141,163..165)
Misc Feature(3)70..270
Misc Feature(4)order(190..192,199..207,211..213,217..219,226..228,244..246,268..270,274..282)
Translation

Target ORF information:

RefSeq Version NM_001180818.1
Organism Saccharomyces cerevisiae S288C
Definition Saccharomyces cerevisiae S288c SUMO family protein SMT3 (SMT3), partial mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001180818.1

ORF Insert Sequence:

1
61
121
181
241
301
ATGTCGGACT CAGAAGTCAA TCAAGAAGCT AAGCCAGAGG TCAAGCCAGA AGTCAAGCCT 
GAGACTCACA TCAATTTAAA GGTGTCCGAT GGATCTTCAG AGATCTTCTT CAAGATCAAA
AAGACCACTC CTTTAAGAAG GCTGATGGAA GCGTTCGCTA AAAGACAGGG TAAGGAAATG
GACTCCTTAA GATTCTTGTA CGACGGTATT AGAATTCAAG CTGATCAGAC CCCTGAAGAT
TTGGACATGG AGGATAACGA TATTATTGAG GCTCACAGAG AACAGATTGG TGGTGCTACG
TATTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Nature387(6632 Suppl)75-8(1997 May)
Jacq C,Alt-Mörbe J,Andre B,Arnold W,Bahr A,Ballesta JP,Bargues M,Baron L,Becker A,Biteau N,Blöcker H,Blugeon C,Boskovic J,Brandt P,Brückner M,Buitrago MJ,Coster F,Delaveau T,del Rey F,Dujon B,Eide LG,Garcia-Cantalejo JM,Goffeau A,Gomez-Peris A,Zaccaria P


book

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


 
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