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TRK1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Summary

Gene Symbol TRK1
Entrez Gene ID 853312
Full Name Trk1p
Gene Type protein-coding
Organism Saccharomyces cerevisiae S288C

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001181562.1 NP_012406.1 Trk1p

Pathways from BioSystems


Homologies


Kluyveromyces lactis KLLA0E15335g XP_454641.1
Saccharomyces cerevisiae (baker's yeast) TRK1 NP_012406.1

Gene Ontology


Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.
Galibert F, Alexandraki D, Baur A, Boles E, Chalwatzis N, Chuat JC, Coster F, Cziepluch C, De Haan M, Domdey H, Durand P, Entian KD, Gatius M, Goffeau A, Grivell LA, Hennemann A, Herbert CJ, Heumann K, Hilger F, Hollenberg CP, Huang ME, Jacq C, Jauniaux JC, Katsoulou C, Karpfinger-Hartl L
The EMBO journal15(9)2031-49(1996 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Using a combination of physiological, biochemical and proteomic approaches, we show that during growth at suboptimal potassium concentrations, double trk1,2 mutants accumulate less potassium and reach lower yields.
Title: A physiological, biochemical and proteomic characterization of Saccharomyces cerevisiae trk1,2 transport mutants grown under limiting potassium conditions.

Refined structural model of the Saccharomyces cerevisiae Trk1 is presented.
Title: A refined atomic scale model of the Saccharomyces cerevisiae K+-translocation protein Trk1p combined with experimental evidence confirms the role of selectivity filter glycines and other key residues.

Trk1 is not essential for adaptation to potassium changes in Saccharomyces cerevisiae.
Title: Role of Saccharomyces cerevisiae Trk1 in stabilization of intracellular potassium content upon changes in external potassium levels.

the Ppz1-Hal3 interaction is a sensor of intracellular pH that modulates H+ and K+ homeostasis through the regulation of Trk1p activity
Title: pH-Responsive, posttranslational regulation of the Trk1 potassium transporter by the type 1-related Ppz1 phosphatase.

Results indicate that, in addition to their role in regulating Trk1 and 2 potassium transporters, Ppz phosphatases 1 and 2 positively affect the residual low affinity potassium transport mechanisms in yeast.
Title: The Ppz protein phosphatases regulate Trk-independent potassium influx in yeast.

The following TRK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi02586 NM_001181562.1
Latest version!		 Saccharomyces cerevisiae S288C Trk1p (TRK1), partial mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $713.30
$1019.00

ORF Online Only Promotion

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1 Clone 30% OFF
2-4 Clone 40% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OSi02586
Clone ID Related Accession (Same CDS sequence) NM_001181562.1
Accession Version NM_001181562.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3708bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-10-31
Organism Saccharomyces cerevisiae S288C
Product Trk1p
Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001142). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGCATTTTA GAAGAACGAT GAGTAGAGTG CCCACATTGG CATCTCTTGA AATACGATAT 
AAAAAATCTT TCGGCCATAA ATTTCGTGAT TTTATTGCTC TATGTGGTCA CTATTTTGCT 
CCAGTTAAAA AATATATCTT CCCCAGTTTT ATCGCGGTTC ACTACTTCTA CACGATATCC 
CTGACATTAA TAACTTCAAT CCTGCTATAT CCCATTAAGA ATACCAGATA CATTGATACA 
TTGTTTTTAG CAGCGGGCGC AGTTACACAA GGTGGCTTAA ATACTGTGGA TATCAACAAT 
CTAAGCTTAT ACCAACAAAT TGTTCTGTAT ATCGTATGCT GCATATCAAC ACCAATTGCA 
GTTCATAGTT GCTTGGCATT TGTACGGCTT TACTGGTTTG AGCGCTACTT CGATGGTATT 
AGAGACTCTT CTAGACGAAA TTTTAAGATG AGAAGAACGA AAACAATCTT AGAAAGGGAA 
CTAACAGCAA GAACCATGAC CAAGAATAGA ACAGGTACCC AAAGAACGTC TTATCCTAGG 
AAACAAGCTA AAACAGATGA TTTCCAAGAA AAATTGTTCA GCGGAGAAAT GGTTAATAGA 
GATGAGCAGG ACTCAGTTCA CAGCGACCAG AATTCTCATG ACATTAGTAG GGACAGCAGC 
AATAATAATA CGAATCACAA TGGTAGCAGT GGCAGTTTAG ATGATTTCGT TAAGGAAGAC 
GAAACGGATG ACAATGGAGA ATATCAGGAG AACAACTCCT ACTCGACGGT AGGTAGTTCG 
TCTAACACAG TTGCAGACGA AAGTTTAAAT CAGAAGCCCA AGCCAAGCAG TCTTCGGTTT 
GATGAGCCAC ACAGCAAACA AAGACCCGCA AGAGTTCCCT CAGAGAAATT TGCAAAAAGA 
AGGGGTTCAA GAGATATTAG CCCAGCCGAT ATGTATCGAT CCATTATGAT GCTACAAGGT 
AAGCATGAAG CAACTGCTGA AGATGAAGGT CCCCCTTTAG TCATCGGGTC CCCTGCGGAT 
GGCACAAGAT ATAAAAGTAA TGTCAATAAG CTAAAGAAGG CCACCGGCAT AAATGGTAAC 
AAAATCAAGA TTCGAGATAA GGGAAATGAA AGTAACACTG ATCAAAATTC CGTGTCAAGT 
GAAGCAAACA GTACGGCGAG CGTTTCGGAC GAAAGCTCGT TACACACAAA TTTTGGTAAC 
AAAGTACCTT CATTAAGAAC AAATACTCAT AGATCAAATT CGGGCCCGAT AGCCATTACT 
GATAACGCAG AAACAGACAA AAAGCATGGG CCATCAATTC AATTCGATAT AACTAAACCT 
CCTAGAAAAA TTTCAAAAAG AGTTTCAACC TTCGATGATT TGAACCCAAA ATCTTCCGTT 
CTTTATCGAA AAAAAGCATC GAAGAAGTAC CTCATGAAAC ATTTTCCTAA AGCGCGGCGA 
ATACGGCAAC AAATTAAGAG AAGGCTTTCT ACTGGTTCAA TTGAGAAAAA CAGCAGTAAC 
AATGTTTCAG ATAGAAAACC TATTACTGAT ATGGATGATG ATGATGATGA CGATGACAAC 
GACGGCGATA ACAACGAAGA ATACTTTGCT GACAACGAAA GCGGCGATGA AGATGAACGA 
GTACAGCAGT CTGAACCACA TTCTGATTCA GAACTCAAAT CGCACCAACA ACAGCAAGAA 
AAACACCAAC TGCAGCAGAA CCTGCACCGC ATGTATAAAA CCAAATCATT TGATGATAAT 
CGTTCAAGAG CAGTTCCTAT GGAACGTTCC AGGACCATCG ATATGGCAGA GGCTAAGGAT 
CTAAATGAGC TCGCAAGGAC GCCTGATTTT CAAAAAATGG TCTATCAAAA TTGGAAAGCC 
CATCATAGAA AAAAACCGAA CTTTAGGAAG AGGGGATGGA ATAACAAGAT ATTTGAACAT 
GGTCCCTATG CATCTGACAG CGATCGCAAT TATCCTGATA ATAGTAATAC TGGAAACAGT 
ATTCTTCATT ACGCAGAGTC TATTTTACAT CATGATGGCT CTCATAAAAA TGGAAGCGAA 
GAAGCCTCTT CCGACTCTAA TGAGAATATC TATTCCACGA ATGGAGGAAG CGACCACAAT 
GGTCTTAACA ACTATCCTAC TTACAACGAC GATGAAGAAG GCTATTATGG TTTACATTTC 
GATACCGATT ATGACCTAGA TCCTCGTCAT GATTTATCTA AAGGCAGTGG TAAAACGTAT 
CTATCATGGC AACCAACTAT TGGACGTAAC TCAAACTTCC TTGGATTAAC AAGAGCCCAG 
AAAGATGAAT TAGGTGGTGT CGAGTACAGA GCAATCAAAC TTTTATGCAC CATATTGGTT 
GTCTACTACG TTGGATGGCA TATTGTTGCT TTTGTTATGT TAGTACCTTG GATTATTTTG 
AAAAAGCATT ATAGTGAAGT TGTTAGAGAT GATGGTGTTT CACCTACATG GTGGGGATTT 
TGGACAGCAA TGAGTGCATT TAATGATTTA GGTTTGACAT TAACTCCAAA TTCAATGATG 
TCGTTTAACA AAGCTGTATA CCCATTGATC GTTATGATTT GGTTTATCAT TATCGGAAAT 
ACAGGGTTTC CCATCCTTCT TAGATGCATC ATTTGGATAA TGTTTAAAAT TTCTCCTGAT 
TTATCACAGA TGAGAGAAAG TTTAGGTTTT CTCTTAGACC ATCCACGTCG TTGTTTCACC 
TTGCTATTTC CTAAGGCAGC TACATGGTGG CTACTTTTAA CGCTTGCAGG ATTGAATATA 
ACTGATTGGA TTTTATTTAT TATTCTAGAT TTTGGCTCAA CAGTTGTGAA ATCATTATCG 
AAAGGCTATA GAGTCCTTGT CGGCCTGTTT CAATCTGTTA GCACAAGAAC TGCTGGATTC 
AGCGTTGTCG ATTTAAGTCA ACTGCATCCT TCTATCCAAG TCTCCTATAT GCTAATGATG 
TATGTCTCCG TATTACCATT GGCCATCTCT ATTCGACGGA CAAATGTTTA CGAGGAGCAA 
TCTTTAGGAC TATATGGAGA TATGGGGGGA GAACCAGAAG ATACGGATAC TGAAGACGAT 
GGTAACGATG AAGATGACGA CGAGGAAAAC GAGAGTCACG AAGGTCAAAG TAGTCAAAGA 
AGTAGTTCGA ACAACAACAA CAATAACAAC AGGAAAAAGA AAAAGAAAAA GAAAACTGAA 
AATCCAAATG AAATATCTAC AAAATCCTTT ATCGGTGCCC ATTTAAGGAA ACAGCTTTCA 
TTTGACTTGT GGTTTCTATT TTTAGGGTTA TTTATCATTT GCATTTGTGA AGGGGACAAG 
ATAAAGGACG TACAAGAACC AAACTTTAAT ATATTTGCAA TTCTTTTTGA AATTGTTAGC 
GCTTACGGTA CAGTTGGGCT ATCGCTAGGT TATCCGGACA CCAACCAATC GTTTTCAAGA 
CAGTTTACTA CATTATCTAA GTTGGTGATC ATAGCTATGC TGATCAGAGG CAAGAATAGA 
GGTCTACCAT ACTCACTGGA TCGTGCAATT ATCTTGCCTA GTGATAGACT TGAACATATT 
GACCACCTTG AGGGCATGAA ATTGAAGAGA CAGGCTAGAA CCAATACAGA AGACCCAATG 
ACGGAACATT TCAAGAGAAG TTTCACTGAT GTGAAACATC GTTGGGGAGC TCTTAAGCGT 
AAGACCACAC ATTCCCGAAA TCCTAAAAGG AGCAGCACAA CGCTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_012406.1
CDS1..3708
Translation

Target ORF information:

RefSeq Version NM_001181562.1
Organism Saccharomyces cerevisiae S288C
Definition Saccharomyces cerevisiae S288C Trk1p (TRK1), partial mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001181562.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGCATTTTA GAAGAACGAT GAGTAGAGTG CCCACATTGG CATCTCTTGA AATACGATAT 
AAAAAATCTT TCGGCCATAA ATTTCGTGAT TTTATTGCTC TATGTGGTCA CTATTTTGCT 
CCAGTTAAAA AATATATCTT CCCCAGTTTT ATCGCGGTTC ACTACTTCTA CACGATATCC 
CTGACATTAA TAACTTCAAT CCTGCTATAT CCCATTAAGA ATACCAGATA CATTGATACA 
TTGTTTTTAG CAGCGGGCGC AGTTACACAA GGTGGCTTAA ATACTGTGGA TATCAACAAT 
CTAAGCTTAT ACCAACAAAT TGTTCTGTAT ATCGTATGCT GCATATCAAC ACCAATTGCA 
GTTCATAGTT GCTTGGCATT TGTACGGCTT TACTGGTTTG AGCGCTACTT CGATGGTATT 
AGAGACTCTT CTAGACGAAA TTTTAAGATG AGAAGAACGA AAACAATCTT AGAAAGGGAA 
CTAACAGCAA GAACCATGAC CAAGAATAGA ACAGGTACCC AAAGAACGTC TTATCCTAGG 
AAACAAGCTA AAACAGATGA TTTCCAAGAA AAATTGTTCA GCGGAGAAAT GGTTAATAGA 
GATGAGCAGG ACTCAGTTCA CAGCGACCAG AATTCTCATG ACATTAGTAG GGACAGCAGC 
AATAATAATA CGAATCACAA TGGTAGCAGT GGCAGTTTAG ATGATTTCGT TAAGGAAGAC 
GAAACGGATG ACAATGGAGA ATATCAGGAG AACAACTCCT ACTCGACGGT AGGTAGTTCG 
TCTAACACAG TTGCAGACGA AAGTTTAAAT CAGAAGCCCA AGCCAAGCAG TCTTCGGTTT 
GATGAGCCAC ACAGCAAACA AAGACCCGCA AGAGTTCCCT CAGAGAAATT TGCAAAAAGA 
AGGGGTTCAA GAGATATTAG CCCAGCCGAT ATGTATCGAT CCATTATGAT GCTACAAGGT 
AAGCATGAAG CAACTGCTGA AGATGAAGGT CCCCCTTTAG TCATCGGGTC CCCTGCGGAT 
GGCACAAGAT ATAAAAGTAA TGTCAATAAG CTAAAGAAGG CCACCGGCAT AAATGGTAAC 
AAAATCAAGA TTCGAGATAA GGGAAATGAA AGTAACACTG ATCAAAATTC CGTGTCAAGT 
GAAGCAAACA GTACGGCGAG CGTTTCGGAC GAAAGCTCGT TACACACAAA TTTTGGTAAC 
AAAGTACCTT CATTAAGAAC AAATACTCAT AGATCAAATT CGGGCCCGAT AGCCATTACT 
GATAACGCAG AAACAGACAA AAAGCATGGG CCATCAATTC AATTCGATAT AACTAAACCT 
CCTAGAAAAA TTTCAAAAAG AGTTTCAACC TTCGATGATT TGAACCCAAA ATCTTCCGTT 
CTTTATCGAA AAAAAGCATC GAAGAAGTAC CTCATGAAAC ATTTTCCTAA AGCGCGGCGA 
ATACGGCAAC AAATTAAGAG AAGGCTTTCT ACTGGTTCAA TTGAGAAAAA CAGCAGTAAC 
AATGTTTCAG ATAGAAAACC TATTACTGAT ATGGATGATG ATGATGATGA CGATGACAAC 
GACGGCGATA ACAACGAAGA ATACTTTGCT GACAACGAAA GCGGCGATGA AGATGAACGA 
GTACAGCAGT CTGAACCACA TTCTGATTCA GAACTCAAAT CGCACCAACA ACAGCAAGAA 
AAACACCAAC TGCAGCAGAA CCTGCACCGC ATGTATAAAA CCAAATCATT TGATGATAAT 
CGTTCAAGAG CAGTTCCTAT GGAACGTTCC AGGACCATCG ATATGGCAGA GGCTAAGGAT 
CTAAATGAGC TCGCAAGGAC GCCTGATTTT CAAAAAATGG TCTATCAAAA TTGGAAAGCC 
CATCATAGAA AAAAACCGAA CTTTAGGAAG AGGGGATGGA ATAACAAGAT ATTTGAACAT 
GGTCCCTATG CATCTGACAG CGATCGCAAT TATCCTGATA ATAGTAATAC TGGAAACAGT 
ATTCTTCATT ACGCAGAGTC TATTTTACAT CATGATGGCT CTCATAAAAA TGGAAGCGAA 
GAAGCCTCTT CCGACTCTAA TGAGAATATC TATTCCACGA ATGGAGGAAG CGACCACAAT 
GGTCTTAACA ACTATCCTAC TTACAACGAC GATGAAGAAG GCTATTATGG TTTACATTTC 
GATACCGATT ATGACCTAGA TCCTCGTCAT GATTTATCTA AAGGCAGTGG TAAAACGTAT 
CTATCATGGC AACCAACTAT TGGACGTAAC TCAAACTTCC TTGGATTAAC AAGAGCCCAG 
AAAGATGAAT TAGGTGGTGT CGAGTACAGA GCAATCAAAC TTTTATGCAC CATATTGGTT 
GTCTACTACG TTGGATGGCA TATTGTTGCT TTTGTTATGT TAGTACCTTG GATTATTTTG 
AAAAAGCATT ATAGTGAAGT TGTTAGAGAT GATGGTGTTT CACCTACATG GTGGGGATTT 
TGGACAGCAA TGAGTGCATT TAATGATTTA GGTTTGACAT TAACTCCAAA TTCAATGATG 
TCGTTTAACA AAGCTGTATA CCCATTGATC GTTATGATTT GGTTTATCAT TATCGGAAAT 
ACAGGGTTTC CCATCCTTCT TAGATGCATC ATTTGGATAA TGTTTAAAAT TTCTCCTGAT 
TTATCACAGA TGAGAGAAAG TTTAGGTTTT CTCTTAGACC ATCCACGTCG TTGTTTCACC 
TTGCTATTTC CTAAGGCAGC TACATGGTGG CTACTTTTAA CGCTTGCAGG ATTGAATATA 
ACTGATTGGA TTTTATTTAT TATTCTAGAT TTTGGCTCAA CAGTTGTGAA ATCATTATCG 
AAAGGCTATA GAGTCCTTGT CGGCCTGTTT CAATCTGTTA GCACAAGAAC TGCTGGATTC 
AGCGTTGTCG ATTTAAGTCA ACTGCATCCT TCTATCCAAG TCTCCTATAT GCTAATGATG 
TATGTCTCCG TATTACCATT GGCCATCTCT ATTCGACGGA CAAATGTTTA CGAGGAGCAA 
TCTTTAGGAC TATATGGAGA TATGGGGGGA GAACCAGAAG ATACGGATAC TGAAGACGAT 
GGTAACGATG AAGATGACGA CGAGGAAAAC GAGAGTCACG AAGGTCAAAG TAGTCAAAGA 
AGTAGTTCGA ACAACAACAA CAATAACAAC AGGAAAAAGA AAAAGAAAAA GAAAACTGAA 
AATCCAAATG AAATATCTAC AAAATCCTTT ATCGGTGCCC ATTTAAGGAA ACAGCTTTCA 
TTTGACTTGT GGTTTCTATT TTTAGGGTTA TTTATCATTT GCATTTGTGA AGGGGACAAG 
ATAAAGGACG TACAAGAACC AAACTTTAAT ATATTTGCAA TTCTTTTTGA AATTGTTAGC 
GCTTACGGTA CAGTTGGGCT ATCGCTAGGT TATCCGGACA CCAACCAATC GTTTTCAAGA 
CAGTTTACTA CATTATCTAA GTTGGTGATC ATAGCTATGC TGATCAGAGG CAAGAATAGA 
GGTCTACCAT ACTCACTGGA TCGTGCAATT ATCTTGCCTA GTGATAGACT TGAACATATT 
GACCACCTTG AGGGCATGAA ATTGAAGAGA CAGGCTAGAA CCAATACAGA AGACCCAATG 
ACGGAACATT TCAAGAGAAG TTTCACTGAT GTGAAACATC GTTGGGGAGC TCTTAAGCGT 
AAGACCACAC ATTCCCGAAA TCCTAAAAGG AGCAGCACAA CGCTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


book

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.
The EMBO journal15(9)2031-49(1996 May)
Galibert F,Alexandraki D,Baur A,Boles E,Chalwatzis N,Chuat JC,Coster F,Cziepluch C,De Haan M,Domdey H,Durand P,Entian KD,Gatius M,Goffeau A,Grivell LA,Hennemann A,Herbert CJ,Heumann K,Hilger F,Hollenberg CP,Huang ME,Jacq C,Jauniaux JC,Katsoulou C,Karpfinger-Hartl L