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SPT5 cDNA ORF clone, Saccharomyces cerevisiae S288C

Summary

Gene Symbol SPT5
Entrez Gene ID 854999
Full Name transcription elongation factor SPT5
Gene Type protein-coding
Organism Saccharomyces cerevisiae S288C

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001182366.1 NP_013703.1 transcription elongation factor SPT5

Pathways from BioSystems


Homologies


Anopheles gambiae (African malaria mosquito) AgaP_AGAP005021 XP_001231045.2
Caenorhabditis elegans (roundworm) spt-5 NP_502283.1
Xenopus tropicalis (tropical clawed frog) supt5h NP_001123796.1
Canis lupus familiaris (dog) SUPT5H XP_005616644.1
Mus musculus (house mouse) Supt5 NP_038704.1
Gallus gallus (chicken) SUPT5H NP_001026555.1
Neurospora crassa NCU06929 XP_959276.2
SUPT5H XP_001088495.1
Bos taurus (cattle) SUPT5H NP_001076908.2
Rattus norvegicus (Norway rat) Supt5h NP_001100967.2
Schizosaccharomyces pombe (fission yeast) spt5 NP_593191.1
Arabidopsis thaliana (thale cress) AT2G34210 NP_180968.2
Oryza sativa (rice) Os02g0772000 NP_001173159.1
Saccharomyces cerevisiae (baker's yeast) SPT5 NP_013703.1
Eremothecium gossypii AGOS_ADR187W NP_984283.2
Homo sapiens (human) SUPT5H NP_001104490.1
Pan troglodytes (chimpanzee) SUPT5H XP_512652.3
Danio rerio (zebrafish) supt5h NP_571748.2
Drosophila melanogaster (fruit fly) Spt5 NP_652610.1
Kluyveromyces lactis KLLA0B00891g XP_451570.1
Magnaporthe oryzae (rice blast fungus) MGG_12268 XP_003715018.1
Arabidopsis thaliana (thale cress) GTA2 NP_192575.3

Gene Ontology


Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Bowman S, Churcher C, Badcock K, Brown D, Chillingworth T, Connor R, Dedman K, Devlin K, Gentles S, Hamlin N, Hunt S, Jagels K, Lye G, Moule S, Odell C, Pearson D, Rajandream M, Rice P, Skelton J, Walsh S, Whitehead S, Barrell B
Nature387(6632 Suppl)90-3(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Spt4/5 may be important to coordinate the mechanical movement of RNAPII through the nucleosome with co-transcriptional chromatin modifications during transcription.
Title: The elongation factor Spt4/5 regulates RNA polymerase II transcription through the nucleosome.

Spt5 is crucial for a normal rate of RNA synthesis and distribution of RNAPII over transcription units.
Title: Spt5 Plays Vital Roles in the Control of Sense and Antisense Transcription Elongation.

demonstrate that physical and genetic interactions exist between Hot1 and several proteins involved in transcriptional and posttranscriptional processes: for example, transcription co-activator Sub1 and elongation complex Spt4/5
Title: Hot1 factor recruits co-activator Sub1 and elongation complex Spt4/5 to osmostress genes.

These findings attribute a new function to a domain of Spt4/5 that associates directly with RNAPII, making significant steps towards elucidating the mechanism behind transcriptional control by Spt4/5.
Title: The yeast transcription elongation factor Spt4/5 is a sequence-specific RNA binding protein.

Interactions between SPT5 and RNA Polymerase II
Title: Relationships Between RNA Polymerase II Activity and Spt Elongation Factors to Spt- Phenotype and Growth in Saccharomyces cerevisiae.

The following SPT5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPT5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi03990 NM_001182366.1
Latest version!		 Saccharomyces cerevisiae S288C transcription elongation factor SPT5 (SPT5), partial mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $629.30
$899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OSi03990
Clone ID Related Accession (Same CDS sequence) NM_001182366.1
Accession Version NM_001182366.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3192bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-10-31
Organism Saccharomyces cerevisiae S288C
Product transcription elongation factor SPT5
Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001145). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
ATGAGTGACA ACTCGGACAC AAACGTGAGC ATGCAGGACC ATGATCAACA ATTTGCTGAT 
CCCGTAGTGG TTCCGCAGTC AACTGACACT AAAGATGAAA ATACTAGTGA CAAAGATACT 
GTTGATAGTG GCAATGTGAC CACAACGGAA AGTACAGAAC GTGCAGAAAG TACAAGCAAT 
ATTCCCCCTT TAGATGGGGA AGAAAAAGAA GCAAAATCTG AGCCACAGCA ACCTGAGGAT 
AATGCAGAAA CGGCGGCCAC AGAGCAGGTT TCATCTAGTA ACGGGCCTGC TACAGATGAT 
GCCCAAGCAA CTTTGAATAC GGATTCATCC GAAGCAAATG AAATTGTCAA GAAGGAAGAG 
GGCTCTGATG AAAGGAAGAG ACCTCGCGAA GAGGACACCA AAAACAGTGA TGGTGATACC 
AAAGATGAGG GCGATAACAA AGATGAAGAC GATGATGAAG ACGACGATGA CGATGATGAT 
GATGAGGACG ATGACGATGA AGCCCCAACT AAAAGGCGTC GTCAGGAGAG GAACAGATTC 
TTGGATATTG AAGCTGAGGT TAGTGATGAT GAAGATGAAG ATGAAGATGA AGAGGATTCA 
GAGTTGGTTC GTGAAGGTTT CATTACCCAT GGTGATGATG AAGATGACGA AGCAAGTGCT 
CCAGGCGCAA GAAGAGACGA TAGATTACAT AGACAACTGG ACCAAGATTT GAACAAGACT 
TCAGAAGAAG ACGCTCAAAG GTTAGCGAAA GAATTAAGGG AGCGTTACGG TAGAAGCAGC 
TCCAAGCAAT ACCGTGCTGC TGCTCAAGAT GGTTACGTGC CCCAGAGGTT TCTCCTACCA 
AGTGTTGATA CAGCTACCAT TTGGGGTGTG CGCTGCAGAC CAGGTAAAGA AAAGGAATTG 
ATTCGTAAGT TATTAAAAAA AAAATTCAAT TTGGATAGGG CGATGGGTAA GAAAAAACTG 
AAAATTTTAT CCATTTTCCA AAGGGATAAT TACACAGGAA GAATCTATAT CGAAGCCCCT 
AAGCAATCCG TTATTGAAAA ATTTTGTAAT GGTGTTCCAG ATATTTATAT TTCTCAAAAA 
TTGCTAATTC CTGTCCAAGA ATTACCTCTA TTACTAAAAC CAAACAAATC TGATGATGTT 
GCTTTGGAAG AAGGTAGCTA CGTTCGTATT AAGAGAGGGA TCTATAAGGG TGACTTAGCT 
ATGGTCGACC AAATTAGTGA GAATAATTTA GAAGTTATGC TGAAAATTGT TCCTCGTCTG 
GATTATGGTA AATTCGACGA AATTGATCCA ACAACACAGC AACGTAAATC CAGAAGACCA 
ACTTTTGCTC ATAGAGCACC ACCGCAATTA TTTAATCCAA CAATGGCTCT AAGATTAGAC 
CAAGCTAACC TGTACAAAAG GGATGATCGC CACTTTACTT ATAAGAATGA AGATTATATC 
GATGGTTATC TGTATAAGTC CTTCAGAATT CAACATGTGG AAACCAAAAA TATTCAGCCA 
ACTGTGGAGG AATTGGCAAG ATTCGGTTCC AAAGAGGGCG CGGTAGATCT AACATCAGTT 
TCACAATCAA TCAAGAAGGC TCAAGCTGCA AAGGTCACTT TCCAGCCAGG TGATCGTATC 
GAAGTTCTAA ATGGTGAACA ACGTGGTTCC AAGGGTATTG TAACAAGAAC CACGAAAGAT 
ATTGCTACTA TCAAACTAAA TGGCTTTACA ACGCCTCTAG AATTTCCTAT CTCTACTCTG 
AGGAAAATTT TCGAACCTGG TGATCACGTT ACTGTCATCA ATGGTGAACA TCAAGGTGAT 
GCTGGTTTAG TTCTTATGGT AGAGCAAGGT CAAGTGACAT TTATGTCAAC TCAAACAAGC 
AGAGAAGTTA CCATTACAGC AAATAATCTG TCCAAATCCA TCGACACTAC AGCTACATCA 
AGTGAATATG CGCTACATGA CATAGTCGAA TTGAGTGCTA AAAATGTTGC TTGTATTATT 
CAAGCTGGCC ACGATATCTT CAAGGTTATT GATGAAACTG GTAAAGTGTC GACAATTACG 
AAGGGGTCTA TCTTGAGTAA AATAAATACT GCTCGTGCAC GCGTTTCTAG TGTCGATGCA 
AATGGTAATG AAATCAAAAT TGGTGATACC ATAGTAGAGA AAGTAGGTTC ACGCAGAGAA 
GGTCAGGTCC TGTACATACA AACACAACAA ATTTTTGTTG TTTCAAAGAA GATTGTTGAA 
AATGCAGGGG TTTTTGTTGT TAACCCTAGT AACGTTGAGG CCGTGGCATC CAAGGACAAT 
ATGTTAAGTA ACAAAATGGA TCTAAGTAAA ATGAATCCAG AAATCATTTC TAAAATGGGA 
CCTCCATCAT CTAAGACATT CCAACAACCC ATCCAGTCCA GAGGAGGTCG TGAAGTTGCA 
CTCGGCAAAA CAGTAAGAAT TCGTTCTGCT GGTTACAAGG GTCAATTAGG TATTGTGAAA 
GATGTGAATG GTGATAAAGC TACTGTCGAA TTACACTCGA AGAACAAACA CATTACAATT 
GACAAGCATA AGTTAACATA TTACAACCGT GAGGGAGGTG AAGGTATCAC GTATGATGAA 
TTGGTTAATA GACGTGGTAG AGTTCCACAG GCCAGAATGG GCCCAAGTTA CGTCAGTGCC 
CCAAGAAACA TGGCCACTGG CGGTATTGCA GCAGGTGCTG CGGCTACCTC TTCTGGTCTT 
AGCGGTGGTA TGACACCAGG ATGGAGCTCC TTCGATGGTG GCAAAACACC AGCTGTAAAT 
GCGCATGGAG GCTCAGGTGG TGGCGGTGTC TCCTCATGGG GTGGTGCTTC CACTTGGGGT 
GGCCAAGGTA ATGGAGGTGC ATCCGCTTGG GGCGGTGCTG GCGGCGGTGC CTCAGCTTGG 
GGCGGCCAAG GTACTGGTGC TACTTCTACT TGGGGTGGTG CTTCAGCCTG GGGTAACAAA 
TCAAGTTGGG GCGGTGCATC CACTTGGGCG TCGGGTGGTG AATCTAATGG TGCCATGTCT 
ACTTGGGGTG GTACCGGTGA TAGGTCAGCC TACGGCGGGG CTTCCACCTG GGGAGGAAAT 
AACAATAATA AAAGTACAAG AGATGGCGGA GCTTCTGCAT GGGGTAACCA AGACGATGGA 
AATAGGTCTG CTTGGAACAA CCAAGGAAAT AAGTCAAACT ATGGTGGTAA CAGTACATGG 
GGAGGTCATT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_013703.1
CDS1..3192
Translation

Target ORF information:

RefSeq Version NM_001182366.1
Organism Saccharomyces cerevisiae S288C
Definition Saccharomyces cerevisiae S288C transcription elongation factor SPT5 (SPT5), partial mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001182366.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
ATGAGTGACA ACTCGGACAC AAACGTGAGC ATGCAGGACC ATGATCAACA ATTTGCTGAT 
CCCGTAGTGG TTCCGCAGTC AACTGACACT AAAGATGAAA ATACTAGTGA CAAAGATACT 
GTTGATAGTG GCAATGTGAC CACAACGGAA AGTACAGAAC GTGCAGAAAG TACAAGCAAT 
ATTCCCCCTT TAGATGGGGA AGAAAAAGAA GCAAAATCTG AGCCACAGCA ACCTGAGGAT 
AATGCAGAAA CGGCGGCCAC AGAGCAGGTT TCATCTAGTA ACGGGCCTGC TACAGATGAT 
GCCCAAGCAA CTTTGAATAC GGATTCATCC GAAGCAAATG AAATTGTCAA GAAGGAAGAG 
GGCTCTGATG AAAGGAAGAG ACCTCGCGAA GAGGACACCA AAAACAGTGA TGGTGATACC 
AAAGATGAGG GCGATAACAA AGATGAAGAC GATGATGAAG ACGACGATGA CGATGATGAT 
GATGAGGACG ATGACGATGA AGCCCCAACT AAAAGGCGTC GTCAGGAGAG GAACAGATTC 
TTGGATATTG AAGCTGAGGT TAGTGATGAT GAAGATGAAG ATGAAGATGA AGAGGATTCA 
GAGTTGGTTC GTGAAGGTTT CATTACCCAT GGTGATGATG AAGATGACGA AGCAAGTGCT 
CCAGGCGCAA GAAGAGACGA TAGATTACAT AGACAACTGG ACCAAGATTT GAACAAGACT 
TCAGAAGAAG ACGCTCAAAG GTTAGCGAAA GAATTAAGGG AGCGTTACGG TAGAAGCAGC 
TCCAAGCAAT ACCGTGCTGC TGCTCAAGAT GGTTACGTGC CCCAGAGGTT TCTCCTACCA 
AGTGTTGATA CAGCTACCAT TTGGGGTGTG CGCTGCAGAC CAGGTAAAGA AAAGGAATTG 
ATTCGTAAGT TATTAAAAAA AAAATTCAAT TTGGATAGGG CGATGGGTAA GAAAAAACTG 
AAAATTTTAT CCATTTTCCA AAGGGATAAT TACACAGGAA GAATCTATAT CGAAGCCCCT 
AAGCAATCCG TTATTGAAAA ATTTTGTAAT GGTGTTCCAG ATATTTATAT TTCTCAAAAA 
TTGCTAATTC CTGTCCAAGA ATTACCTCTA TTACTAAAAC CAAACAAATC TGATGATGTT 
GCTTTGGAAG AAGGTAGCTA CGTTCGTATT AAGAGAGGGA TCTATAAGGG TGACTTAGCT 
ATGGTCGACC AAATTAGTGA GAATAATTTA GAAGTTATGC TGAAAATTGT TCCTCGTCTG 
GATTATGGTA AATTCGACGA AATTGATCCA ACAACACAGC AACGTAAATC CAGAAGACCA 
ACTTTTGCTC ATAGAGCACC ACCGCAATTA TTTAATCCAA CAATGGCTCT AAGATTAGAC 
CAAGCTAACC TGTACAAAAG GGATGATCGC CACTTTACTT ATAAGAATGA AGATTATATC 
GATGGTTATC TGTATAAGTC CTTCAGAATT CAACATGTGG AAACCAAAAA TATTCAGCCA 
ACTGTGGAGG AATTGGCAAG ATTCGGTTCC AAAGAGGGCG CGGTAGATCT AACATCAGTT 
TCACAATCAA TCAAGAAGGC TCAAGCTGCA AAGGTCACTT TCCAGCCAGG TGATCGTATC 
GAAGTTCTAA ATGGTGAACA ACGTGGTTCC AAGGGTATTG TAACAAGAAC CACGAAAGAT 
ATTGCTACTA TCAAACTAAA TGGCTTTACA ACGCCTCTAG AATTTCCTAT CTCTACTCTG 
AGGAAAATTT TCGAACCTGG TGATCACGTT ACTGTCATCA ATGGTGAACA TCAAGGTGAT 
GCTGGTTTAG TTCTTATGGT AGAGCAAGGT CAAGTGACAT TTATGTCAAC TCAAACAAGC 
AGAGAAGTTA CCATTACAGC AAATAATCTG TCCAAATCCA TCGACACTAC AGCTACATCA 
AGTGAATATG CGCTACATGA CATAGTCGAA TTGAGTGCTA AAAATGTTGC TTGTATTATT 
CAAGCTGGCC ACGATATCTT CAAGGTTATT GATGAAACTG GTAAAGTGTC GACAATTACG 
AAGGGGTCTA TCTTGAGTAA AATAAATACT GCTCGTGCAC GCGTTTCTAG TGTCGATGCA 
AATGGTAATG AAATCAAAAT TGGTGATACC ATAGTAGAGA AAGTAGGTTC ACGCAGAGAA 
GGTCAGGTCC TGTACATACA AACACAACAA ATTTTTGTTG TTTCAAAGAA GATTGTTGAA 
AATGCAGGGG TTTTTGTTGT TAACCCTAGT AACGTTGAGG CCGTGGCATC CAAGGACAAT 
ATGTTAAGTA ACAAAATGGA TCTAAGTAAA ATGAATCCAG AAATCATTTC TAAAATGGGA 
CCTCCATCAT CTAAGACATT CCAACAACCC ATCCAGTCCA GAGGAGGTCG TGAAGTTGCA 
CTCGGCAAAA CAGTAAGAAT TCGTTCTGCT GGTTACAAGG GTCAATTAGG TATTGTGAAA 
GATGTGAATG GTGATAAAGC TACTGTCGAA TTACACTCGA AGAACAAACA CATTACAATT 
GACAAGCATA AGTTAACATA TTACAACCGT GAGGGAGGTG AAGGTATCAC GTATGATGAA 
TTGGTTAATA GACGTGGTAG AGTTCCACAG GCCAGAATGG GCCCAAGTTA CGTCAGTGCC 
CCAAGAAACA TGGCCACTGG CGGTATTGCA GCAGGTGCTG CGGCTACCTC TTCTGGTCTT 
AGCGGTGGTA TGACACCAGG ATGGAGCTCC TTCGATGGTG GCAAAACACC AGCTGTAAAT 
GCGCATGGAG GCTCAGGTGG TGGCGGTGTC TCCTCATGGG GTGGTGCTTC CACTTGGGGT 
GGCCAAGGTA ATGGAGGTGC ATCCGCTTGG GGCGGTGCTG GCGGCGGTGC CTCAGCTTGG 
GGCGGCCAAG GTACTGGTGC TACTTCTACT TGGGGTGGTG CTTCAGCCTG GGGTAACAAA 
TCAAGTTGGG GCGGTGCATC CACTTGGGCG TCGGGTGGTG AATCTAATGG TGCCATGTCT 
ACTTGGGGTG GTACCGGTGA TAGGTCAGCC TACGGCGGGG CTTCCACCTG GGGAGGAAAT 
AACAATAATA AAAGTACAAG AGATGGCGGA GCTTCTGCAT GGGGTAACCA AGACGATGGA 
AATAGGTCTG CTTGGAACAA CCAAGGAAAT AAGTCAAACT ATGGTGGTAA CAGTACATGG 
GGAGGTCATT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Nature387(6632 Suppl)90-3(1997 May)
Bowman S,Churcher C,Badcock K,Brown D,Chillingworth T,Connor R,Dedman K,Devlin K,Gentles S,Hamlin N,Hunt S,Jagels K,Lye G,Moule S,Odell C,Pearson D,Rajandream M,Rice P,Skelton J,Walsh S,Whitehead S,Barrell B


book

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG