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DDI3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene Symbol DDI3
Entrez Gene ID 855381
Full Name cyanamide hydratase
Gene Type protein-coding
Organism Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA Protein Name
NM_001183173.1 NP_014064.1 cyanamide hydratase



Neurospora crassa NCU04466 XP_957035.2
Saccharomyces cerevisiae (baker's yeast) DDI3 NP_014064.1
Saccharomyces cerevisiae (baker's yeast) DDI2 NP_116594.1


Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Philippsen P, Kleine K, Pöhlmann R, Düsterhöft A, Hamberg K, Hegemann JH, Obermaier B, Urrestarazu LA, Aert R, Albermann K, Altmann R, André B, Baladron V, Ballesta JP, Bécam AM, Beinhauer J, Boskovic J, Buitrago MJ, Bussereau F, Coster F, Crouzet M, D'Angelo M, Dal Pero F, De Antoni A, Del Rey F, Doignon F, Domdey H, Dubois E, Fiedler T, Fleig U, Floeth M, Fritz C, Gaillardin C, Garcia-Cantalejo JM, Glansdorff NN, Goffeau A, Gueldener U, Herbert C, Heumann K, Heuss-Neitzel D, Hilbert H, Hinni K, Iraqui Houssaini I, Jacquet M, Jimenez A, Jonniaux JL, Karpfinger L, Lanfranchi G, Lepingle A, Levesque H, Lyck R, Maftahi M, Mallet L, Maurer KC, Messenguy F, Mewes HW, Mösti D, Nasr F, Nicaud JM, Niedenthal RK, Pandolfo D, Piérard A, Piravandi E, Planta RJ, Pohl TM, Purnelle B, Rebischung C, Remacha M, Revuelta JL, Rinke M, Saiz JE, Sartorello F, Scherens B, Sen-Gupta M, Soler-Mira A, Urbanus JH, Valle G, Van Dyck L, Verhasselt P, Vierendeels F, Vissers S, Voet M, Volckaert G, Wach A, Wambutt R, Wedler H, Zollner A, Hani J
Nature387(6632 Suppl)93-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DDI2/3 genes protect host cells from cyanamide toxicity.
Title: Two duplicated genes DDI2 and DDI3 in budding yeast encode a cyanamide hydratase and are induced by cyanamide.

The following DDI3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DDI3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OSi04319 NM_001183173.1
Latest version!
Saccharomyces cerevisiae S288c cyanamide hydratase (DDI3), partial mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $174.30
$249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OSi04319
Clone ID Related Accession (Same CDS sequence) NM_001183173.1
Accession Version NM_001183173.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 678bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-03-15
Organism Saccharomyces cerevisiae S288C
Product cyanamide hydratase
Comment REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001146). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

1
61
121
181
241
301
361
421
481
541
601
661
ATGTCACAGT ACGGATTTGT AAGAGTTCCT AGAGAGGTAG AAAAGGCCAT TCCAGTGGTG 
AATGCACCTA GACCACGGGC CGTTGTTCCG CCTCCAAACA GTGAAACTGC TAGGCTTGTT
CGGGAATATG CCGCTAAAGA ATTGACTGCC CCCGTTCTAA ACCACTCTTT GCGTGTTTTT
CAATATAGTG TAGCTATCAT AAGAGACCAA TTTCCAGCAT GGGACTTGGA TCAGGAAGTT
TTGTACGTCA CCTGCTTACT TCATGATATT GCAACAACAG ATAAGAATAT GAGAGCCACG
AAGATGTCAT TTGAGTATTA TGGTGGCATA CTTTCAAGGG AGCTTGTATT TAATGCGACA
GGTGGAAATC AGGACTACGC AGATGCAGTA ACTGAGGCCA TCATTCGTCA CCAGGATTTG
ACTGGGACTG GCTACATTAC CACCTTGGGG CTCATTCTGC AGATTGCTAC TACGCTTGAC
AATGTCGGAT CCAATACCGA TCTGATTCAT ATCGATACAG TTAGTGCCAT TAACGAGCAA
TTTCCAAGAC TGCACTGGTT ATCATGTTTT GCTACGGTGG TGGACACTGA AAACTCGAGA
AAACCGTGGG GCCACACCAG TTCTTTGGGT GATGATTTTT CAAAGAAAGT CATATGCAAT
ACATTTGGGT ATAACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_014064.1
CDS1..678
Misc Feature(1)4..672
Misc Feature(2)order(163..165,262..267,469..471)
Misc Feature(3)265..267
Translation

Target ORF information:

RefSeq Version NM_001183173.1
Organism Saccharomyces cerevisiae S288C
Definition Saccharomyces cerevisiae S288c cyanamide hydratase (DDI3), partial mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001183173.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGTCACAGT ACGGATTTGT AAGAGTTCCT AGAGAGGTAG AAAAGGCCAT TCCAGTGGTG 
AATGCACCTA GACCACGGGC CGTTGTTCCG CCTCCAAACA GTGAAACTGC TAGGCTTGTT
CGGGAATATG CCGCTAAAGA ATTGACTGCC CCCGTTCTAA ACCACTCTTT GCGTGTTTTT
CAATATAGTG TAGCTATCAT AAGAGACCAA TTTCCAGCAT GGGACTTGGA TCAGGAAGTT
TTGTACGTCA CCTGCTTACT TCATGATATT GCAACAACAG ATAAGAATAT GAGAGCCACG
AAGATGTCAT TTGAGTATTA TGGTGGCATA CTTTCAAGGG AGCTTGTATT TAATGCGACA
GGTGGAAATC AGGACTACGC AGATGCAGTA ACTGAGGCCA TCATTCGTCA CCAGGATTTG
ACTGGGACTG GCTACATTAC CACCTTGGGG CTCATTCTGC AGATTGCTAC TACGCTTGAC
AATGTCGGAT CCAATACCGA TCTGATTCAT ATCGATACAG TTAGTGCCAT TAACGAGCAA
TTTCCAAGAC TGCACTGGTT ATCATGTTTT GCTACGGTGG TGGACACTGA AAACTCGAGA
AAACCGTGGG GCCACACCAG TTCTTTGGGT GATGATTTTT CAAAGAAAGT CATATGCAAT
ACATTTGGGT ATAACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Nature387(6632 Suppl)93-8(1997 May)
Philippsen P,Kleine K,Pöhlmann R,Düsterhöft A,Hamberg K,Hegemann JH,Obermaier B,Urrestarazu LA,Aert R,Albermann K,Altmann R,André B,Baladron V,Ballesta JP,Bécam AM,Beinhauer J,Boskovic J,Buitrago MJ,Bussereau F,Coster F,Crouzet M,D'Angelo M,Dal Pero F,De Antoni A,Del Rey F,Doignon F,Domdey H,Dubois E,Fiedler T,Fleig U,Floeth M,Fritz C,Gaillardin C,Garcia-Cantalejo JM,Glansdorff NN,Goffeau A,Gueldener U,Herbert C,Heumann K,Heuss-Neitzel D,Hilbert H,Hinni K,Iraqui Houssaini I,Jacquet M,Jimenez A,Jonniaux JL,Karpfinger L,Lanfranchi G,Lepingle A,Levesque H,Lyck R,Maftahi M,Mallet L,Maurer KC,Messenguy F,Mewes HW,Mösti D,Nasr F,Nicaud JM,Niedenthal RK,Pandolfo D,Piérard A,Piravandi E,Planta RJ,Pohl TM,Purnelle B,Rebischung C,Remacha M,Revuelta JL,Rinke M,Saiz JE,Sartorello F,Scherens B,Sen-Gupta M,Soler-Mira A,Urbanus JH,Valle G,Van Dyck L,Verhasselt P,Vierendeels F,Vissers S,Voet M,Volckaert G,Wach A,Wambutt R,Wedler H,Zollner A,Hani J


book

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


 
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