SPBC646.03 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following SPBC646.03 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPBC646.03 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc101082 NM_001021269.2
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Schizosaccharomyces pombe putative glutamyl-tRNA amidotransferase alpha subunit (SPBC646.03), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSc101082
    Clone ID Related Accession (Same CDS sequence) NM_001021269.2
    Accession Version NM_001021269.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1416bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product putative glutamyl-tRNA amidotransferase alpha subunit
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001021269.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGAGTAATA AATATAACAA TTTATTAAAA GAAGTTGCAG CTAAATATGC TAGTATCATT 
    CTAAATGATG CGAAAATTAA GTCTTTAACT TCAATAAATA GTGCCGAATA TCTATATGAT
    TCATTTGTTG AAATTAGTAA ATTACCACTG GAAAAGAAGC TTCCGTTAAA ATGGAAACTG
    ATTACAGTAA AGGAAAATAT TTGCACGAAG ACAAATTTAA CCACGGCAGC CTCTAATATG
    TTGAAGGACT ACAATTCTCC GTTTGATGCG TCAATTGTCG AAAGCTTGAA GAAAGCCGGC
    GGTATTATTC TTGGTAAAAC AAATATGGAT GAGTTTGCAA TGGGAGTCAA GAGCGAAAAC
    AATCTTTTTG GTCGCACTGT TAATCCTGTT GTTAAGGATA GTAATTACGA TGTTGGGGGC
    AGCTCGGGAG GAGCTGCTGC TGCAATCGCG GCGGATATAT GTTATGCATC AGTTGGTTCA
    GACACAGGAG GATCAATCCG TCTACCTGCT GCTTACGTAG GTTGTGTAGG TTTTAAACCA
    TCTTTCGGCA GGATTTCGAG ATATGGAATG CTTGCCTTTG CCAATAGTTT TGACACAGTG
    GGTATCGCTG CAAATAATGT TAAGGGCGTT ACCAAAGTAT TTAATGTTTT AGACCATCCC
    GACATAAATG ACTCAACCTG TTTAACTAAA GAAGCTCGTT ATTTTGTTAA AGAGCAGCAC
    AAAAAATTAT CGCGGAAGCC AATTAAAATA GGAATTCCAA TTGACTGGAA TGTTTCTGAG
    ACTCATCCAA ACGTTTTAGA TAAGTGGAAT GAATTCATAT CTCTTCTGAA ATCAAATGGT
    TATCTTGTCC AAGAAATACA GCTTCCAATT TCATTATACG CTAACAGTGT ATATTCCACA
    ATGGCTTATG CAGAAGCGAC TTCAAATTTG GCTAAATATA ATACTATCGC ATTTGGCAAT
    TGTTTAGATG AAAAGTTCGA AGAGGAAATA ATTTCATCTA CTGCACGCTC TTTTTTTTTA
    GGAGACGAAG TGAAAAAGAG GCTACTGCTA GGCGCTTATT CACTAGCAAG AATGAATTCC
    TCTGATTTGT TTTCTAAGGC TCGATACGTT CGCCGAGCCA TACAGCTGGA ATTCAACAAA
    AACTTTTTTC TACCTTCCTT TTCTGTCGAT GATCCCAGGG GTGACATCGA TTTCATAGTT
    ACCCCTTCTT TTTTTAATTC CAGTCAACCC ATTGAAACCC CGTCCTCTTA TAGTCATCTT
    TCAGATACTA TGTTAGTTCC AGCGAATATG GCTGGAATAC CATCCGTTAG CATACCTTTC
    GGTACTTTGA ACAATGGGCT TCCAATGGGA ATTCAAATTA TGGCCCAATA TCTTAATGAC
    GAAGATTTGT TGTCTTTTGC AGGCCAGTTT GCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_595361.1
    CDS91..1506
    Translation

    Target ORF information:

    RefSeq Version NM_001021269.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe putative glutamyl-tRNA amidotransferase alpha subunit (SPBC646.03), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001021269.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGAGTAATA AATATAACAA TTTATTAAAA GAAGTTGCAG CTAAATATGC TAGTATCATT 
    CTAAATGATG CGAAAATTAA GTCTTTAACT TCAATAAATA GTGCCGAATA TCTATATGAT
    TCATTTGTTG AAATTAGTAA ATTACCACTG GAAAAGAAGC TTCCGTTAAA ATGGAAACTG
    ATTACAGTAA AGGAAAATAT TTGCACGAAG ACAAATTTAA CCACGGCAGC CTCTAATATG
    TTGAAGGACT ACAATTCTCC GTTTGATGCG TCAATTGTCG AAAGCTTGAA GAAAGCCGGC
    GGTATTATTC TTGGTAAAAC AAATATGGAT GAGTTTGCAA TGGGAGTCAA GAGCGAAAAC
    AATCTTTTTG GTCGCACTGT TAATCCTGTT GTTAAGGATA GTAATTACGA TGTTGGGGGC
    AGCTCGGGAG GAGCTGCTGC TGCAATCGCG GCGGATATAT GTTATGCATC AGTTGGTTCA
    GACACAGGAG GATCAATCCG TCTACCTGCT GCTTACGTAG GTTGTGTAGG TTTTAAACCA
    TCTTTCGGCA GGATTTCGAG ATATGGAATG CTTGCCTTTG CCAATAGTTT TGACACAGTG
    GGTATCGCTG CAAATAATGT TAAGGGCGTT ACCAAAGTAT TTAATGTTTT AGACCATCCC
    GACATAAATG ACTCAACCTG TTTAACTAAA GAAGCTCGTT ATTTTGTTAA AGAGCAGCAC
    AAAAAATTAT CGCGGAAGCC AATTAAAATA GGAATTCCAA TTGACTGGAA TGTTTCTGAG
    ACTCATCCAA ACGTTTTAGA TAAGTGGAAT GAATTCATAT CTCTTCTGAA ATCAAATGGT
    TATCTTGTCC AAGAAATACA GCTTCCAATT TCATTATACG CTAACAGTGT ATATTCCACA
    ATGGCTTATG CAGAAGCGAC TTCAAATTTG GCTAAATATA ATACTATCGC ATTTGGCAAT
    TGTTTAGATG AAAAGTTCGA AGAGGAAATA ATTTCATCTA CTGCACGCTC TTTTTTTTTA
    GGAGACGAAG TGAAAAAGAG GCTACTGCTA GGCGCTTATT CACTAGCAAG AATGAATTCC
    TCTGATTTGT TTTCTAAGGC TCGATACGTT CGCCGAGCCA TACAGCTGGA ATTCAACAAA
    AACTTTTTTC TACCTTCCTT TTCTGTCGAT GATCCCAGGG GTGACATCGA TTTCATAGTT
    ACCCCTTCTT TTTTTAATTC CAGTCAACCC ATTGAAACCC CGTCCTCTTA TAGTCATCTT
    TCAGATACTA TGTTAGTTCC AGCGAATATG GCTGGAATAC CATCCGTTAG CATACCTTTC
    GGTACTTTGA ACAATGGGCT TCCAATGGGA ATTCAAATTA TGGCCCAATA TCTTAATGAC
    GAAGATTTGT TGTCTTTTGC AGGCCAGTTT GCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L


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