HECTD1 cDNA ORF clone, Sus scrofa(pig)

  • Gene

  • Clones

  • gRNAs

The following HECTD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HECTD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSe223525 XM_021099884.1
Latest version!
Sus scrofa HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OSe14103 XM_013978136.1
Latest version!
Sus scrofa HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OSe223517 XM_013978136.2
Latest version!
Sus scrofa HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OSe223518 XM_021099877.1
Latest version!
Sus scrofa HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OSe223519 XM_021099878.1
Latest version!
Sus scrofa HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OSe223520 XM_021099879.1
Latest version!
Sus scrofa HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OSe223521 XM_021099880.1
Latest version!
Sus scrofa HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OSe223522 XM_021099881.1
Latest version!
Sus scrofa HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OSe223523 XM_021099882.1
Latest version!
Sus scrofa HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OSe223524 XM_021099883.1
Latest version!
Sus scrofa HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSe223525
    Clone ID Related Accession (Same CDS sequence) XM_021099884.1
    Accession Version XM_021099884.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5400bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010449.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGCAGATG TGGATCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAGATT
    TTTCTTGATG AAAGTGCTCC AGACAACGTG TTAGAGGTGA CCGCCCGTGC CATAACATAC
    TACCTGGACG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTGGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAATGTG TGAAGGTGTT AGAACTGATA TGCACTCGTG AGTCAGGAGC GGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACACCT GGTTCATAAA
    GATACATTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCGTT AGCCAAGCAT GGATTAACTG AGGAATTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT ATCAGGGCCA TCATCTGCTT GCAAACCAGG TCGCAGCACC
    ACGGGAGCGC CTTCCACGGC TGCAGACTCC AAATTGAGTA ACCAGGTGTC AACAATTGTG
    AGCCTGCTCT CAACACTTTG TAGAGGCTCT CCGGTAGTAA CACATGATCT CCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGACG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAGGC TTTGCCAAAG
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTCCGGA GATTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GGGGTCAGAG GTCATCTTCT TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTCTT AAGGCATGGT GCAAACCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAAAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGACACA
    AACAAAGATG AAGAAGAATG TAATGAGCCA AAAGGAGATC CAGAAATGGC ACCCATATAC
    TTGAAAAGGT TACTGCCAGT ATTTGCACAA ACATTTCAGC AGACGATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT TATTCGAAAA ATGATTCATT TTTGTTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCGA CAGTACTAGT GGAGATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGTTGGCTCT GCAGATCATA
    AGAGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACATT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAAGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA GGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT ATGGAGTGAT
    GCGGCAGCCT TGGAATTATC CAATGGAAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GCTACAAAGA GCTCGAGGCC AAGTAAAGCC TTCTACTTCA
    AGTCAGCCTA TACTCTCAGC ACCAGGACCT ACTAAACTCA CTGTAGGGAA TTGGTCACTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCAACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTGTTT GAGTCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA GAGAGGCAGA
    AAACTAAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACCATGGC TCGAGATTTA
    TATGATGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTGATGAAG ACAGCTTTAG AAAATCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATACGAA ATGTGCAGTA GTGGCTTGGT GCAAGCACTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTAGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTC AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTGCTGGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTGCAGA TACTTACAAG AAGATTAAGA
    TTTCGGTTAG AACGTGCACC TGGTGAAACT GCACTAATAG ACAGAACTGG CAGGATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTAGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ATGATTTCGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCGT TTACTGGATT
    GGGACAAATG CCAAAACTGC ATATGAATGG GTAAATCCAG CTGCATATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ATTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCTTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTA
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AAGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGGAAG
    AATGCTAGTG GACAAACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTC
    AACGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAGGC TAATCTTAGA
    AGACAGAGAC GTCTGGTACG CTCCCAGGTA TTGAAGTACA TGGTTCCAGG GGCTCGTGTT
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACC
    GTCACAGGAG AGCTGCACAA TGCTTCTCCT CTTATGGGCG CTCAGAGTTT CCCTAATTTG
    ACCACACCTG GTACTACATC AACAGTGACT ATGTCAACGT CCAGTGTTAC CAGTAGTAGC
    AATGTAGCTA CAGCAACAAC AGTTTTATCA GTTGGTCAGT CATTAAGTAA TACTTTAACC
    ACCAGCCTCA CATCAACCTC CAGTGAGAGT GACACAGGTC AGGAAGCAGA ATATTCCTTA
    TATGATTTCC TTGATAGCTG CCGTGCCAGT ACTCTGTTAG CTGAGCTAGA TGACGATGAG
    GACTTGCCAG AACCAGATGA GGAAGATGAT GAAAATGAAG ATGACAATCA GGAGGACCAA
    GAATACGAGG AGGTTATGAT TCTGAGACGT CCATCTCTGC AGCGTCGAGC TGGCTCCCGC
    TCTGATGTGA CACACCATGC TGTCACCTCA CAGCTACCAC AGGTCCCTGC TGGAGCAGGG
    AGCCGACCTA TTGGGGAGCA GGAAGAAGAA GAGTATGAAA CTAAAGGGGG ACGCCGGAGA
    ACATGGGATG ATGATTATGT GCTAAAGCGG CAGTTTTCAG CTTTGGTTCC TGCTTTTGAT
    CCTAGACCTG GTCGTACTAA TGTACAGCAG ACAACTGATT TAGAAATTCC GCCCCCAGGA
    ACACCTCATT CAGAGCTCTT GGAAGAAGTT GAATGTACTC CATCACCTCG ATTAGCCCTC
    ACTTTGAAAG TAACAGGTCT TGGAACAACT CGTGAGGTTG AATTACCACT CACTAATTTC
    AGATCAACCA TCTTTTACTA TGTACAGAAA CTGCTTCAGT TGTCCTGTAA TGGCAATGTG
    AAATCAGATA AACTTAGGCG TATTTGGGAG CCAACATACA CAATTATGTA CAGAGAAATG
    AAGGATTCTG ACAAAGAAAA AGAAAATGGA AAAATGGACT TTTGTGAACA TGGAACTAAG
    TCTGGGTTAA ACCAGGGGGC CATTTCTACT CTTCAAAGTA GTGATATTCT TAACTTGACA
    AAAGAACAAC CTCAGGCCAA AGCAGGCAAT GGACAGAATT CTTGTGGAGT AGAAGACGTC
    CTTCAGCTTC TGCGTATTCT GTATATAGTT GCAAGTGACC CTTATTCAAG AATATCCCAA
    GAAGAAGGTG ATGAGCAGCC TCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA
    ATCACAACAA AAATTTTGCA GCAAATTGAG GAACCATTGG CATTGGCAAG TGGCGCTCTT
    CCAGACTGGT GTGAACAACT AACCAGCAAG TGTCCTTTTC TAATTCCGTT TGAAACTAGG
    CAGCTTTACT TCACATGTAC GGCGTTTGGT GCATCCAGAG CAATCGTGTG GTTACAAAAC
    CGACGTGAGG CCACTGTAGA AAGAACAAGG ACCACAAGTA GTGTAAGACG AGATGATCCT
    GGAGAGTTTC GAGTTGGTCG TCTCAAGCAT GAAAGAGTGA AAGTTCCACG TGGTGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020955543.1
    CDS802..6201
    Translation

    Target ORF information:

    RefSeq Version XM_021099884.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021099884.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGCAGATG TGGATCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAGATT
    TTTCTTGATG AAAGTGCTCC AGACAACGTG TTAGAGGTGA CCGCCCGTGC CATAACATAC
    TACCTGGACG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTGGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAATGTG TGAAGGTGTT AGAACTGATA TGCACTCGTG AGTCAGGAGC GGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACACCT GGTTCATAAA
    GATACATTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCGTT AGCCAAGCAT GGATTAACTG AGGAATTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT ATCAGGGCCA TCATCTGCTT GCAAACCAGG TCGCAGCACC
    ACGGGAGCGC CTTCCACGGC TGCAGACTCC AAATTGAGTA ACCAGGTGTC AACAATTGTG
    AGCCTGCTCT CAACACTTTG TAGAGGCTCT CCGGTAGTAA CACATGATCT CCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGACG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAGGC TTTGCCAAAG
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTCCGGA GATTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GGGGTCAGAG GTCATCTTCT TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTCTT AAGGCATGGT GCAAACCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAAAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGACACA
    AACAAAGATG AAGAAGAATG TAATGAGCCA AAAGGAGATC CAGAAATGGC ACCCATATAC
    TTGAAAAGGT TACTGCCAGT ATTTGCACAA ACATTTCAGC AGACGATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT TATTCGAAAA ATGATTCATT TTTGTTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCGA CAGTACTAGT GGAGATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGTTGGCTCT GCAGATCATA
    AGAGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACATT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAAGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA GGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT ATGGAGTGAT
    GCGGCAGCCT TGGAATTATC CAATGGAAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GCTACAAAGA GCTCGAGGCC AAGTAAAGCC TTCTACTTCA
    AGTCAGCCTA TACTCTCAGC ACCAGGACCT ACTAAACTCA CTGTAGGGAA TTGGTCACTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCAACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTGTTT GAGTCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA GAGAGGCAGA
    AAACTAAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACCATGGC TCGAGATTTA
    TATGATGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTGATGAAG ACAGCTTTAG AAAATCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATACGAA ATGTGCAGTA GTGGCTTGGT GCAAGCACTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTAGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTC AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTGCTGGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTGCAGA TACTTACAAG AAGATTAAGA
    TTTCGGTTAG AACGTGCACC TGGTGAAACT GCACTAATAG ACAGAACTGG CAGGATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTAGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ATGATTTCGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCGT TTACTGGATT
    GGGACAAATG CCAAAACTGC ATATGAATGG GTAAATCCAG CTGCATATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ATTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCTTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTA
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AAGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGGAAG
    AATGCTAGTG GACAAACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTC
    AACGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAGGC TAATCTTAGA
    AGACAGAGAC GTCTGGTACG CTCCCAGGTA TTGAAGTACA TGGTTCCAGG GGCTCGTGTT
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACC
    GTCACAGGAG AGCTGCACAA TGCTTCTCCT CTTATGGGCG CTCAGAGTTT CCCTAATTTG
    ACCACACCTG GTACTACATC AACAGTGACT ATGTCAACGT CCAGTGTTAC CAGTAGTAGC
    AATGTAGCTA CAGCAACAAC AGTTTTATCA GTTGGTCAGT CATTAAGTAA TACTTTAACC
    ACCAGCCTCA CATCAACCTC CAGTGAGAGT GACACAGGTC AGGAAGCAGA ATATTCCTTA
    TATGATTTCC TTGATAGCTG CCGTGCCAGT ACTCTGTTAG CTGAGCTAGA TGACGATGAG
    GACTTGCCAG AACCAGATGA GGAAGATGAT GAAAATGAAG ATGACAATCA GGAGGACCAA
    GAATACGAGG AGGTTATGAT TCTGAGACGT CCATCTCTGC AGCGTCGAGC TGGCTCCCGC
    TCTGATGTGA CACACCATGC TGTCACCTCA CAGCTACCAC AGGTCCCTGC TGGAGCAGGG
    AGCCGACCTA TTGGGGAGCA GGAAGAAGAA GAGTATGAAA CTAAAGGGGG ACGCCGGAGA
    ACATGGGATG ATGATTATGT GCTAAAGCGG CAGTTTTCAG CTTTGGTTCC TGCTTTTGAT
    CCTAGACCTG GTCGTACTAA TGTACAGCAG ACAACTGATT TAGAAATTCC GCCCCCAGGA
    ACACCTCATT CAGAGCTCTT GGAAGAAGTT GAATGTACTC CATCACCTCG ATTAGCCCTC
    ACTTTGAAAG TAACAGGTCT TGGAACAACT CGTGAGGTTG AATTACCACT CACTAATTTC
    AGATCAACCA TCTTTTACTA TGTACAGAAA CTGCTTCAGT TGTCCTGTAA TGGCAATGTG
    AAATCAGATA AACTTAGGCG TATTTGGGAG CCAACATACA CAATTATGTA CAGAGAAATG
    AAGGATTCTG ACAAAGAAAA AGAAAATGGA AAAATGGACT TTTGTGAACA TGGAACTAAG
    TCTGGGTTAA ACCAGGGGGC CATTTCTACT CTTCAAAGTA GTGATATTCT TAACTTGACA
    AAAGAACAAC CTCAGGCCAA AGCAGGCAAT GGACAGAATT CTTGTGGAGT AGAAGACGTC
    CTTCAGCTTC TGCGTATTCT GTATATAGTT GCAAGTGACC CTTATTCAAG AATATCCCAA
    GAAGAAGGTG ATGAGCAGCC TCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA
    ATCACAACAA AAATTTTGCA GCAAATTGAG GAACCATTGG CATTGGCAAG TGGCGCTCTT
    CCAGACTGGT GTGAACAACT AACCAGCAAG TGTCCTTTTC TAATTCCGTT TGAAACTAGG
    CAGCTTTACT TCACATGTAC GGCGTTTGGT GCATCCAGAG CAATCGTGTG GTTACAAAAC
    CGACGTGAGG CCACTGTAGA AAGAACAAGG ACCACAAGTA GTGTAAGACG AGATGATCCT
    GGAGAGTTTC GAGTTGGTCG TCTCAAGCAT GAAAGAGTGA AAGTTCCACG TGGTGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe14103
    Clone ID Related Accession (Same CDS sequence) XM_013978136.1
    Accession Version XM_013978136.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7833bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-10
    Organism Sus scrofa(Pig)
    Product LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase HECTD1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003610680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 3 indels ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGATCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAGATT
    TTTCTTGATG AAAGTGCTCC AGACAACGTG TTAGAGGTGA CCGCCCGTGC CATAACATAC
    TACCTGGACG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTGGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAATGTG TGAAGGTGTT AGAACTGATA TGCACTCGTG AGTCAGGAGC GGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACACCT GGTTCATAAA
    GATACCTTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCGTT AGCCAAGCAT GGATTAACTG AGGAATTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT ATCAGGGCCA TCATCTGCTT GCAAACCAGG TCGCAGCACC
    ACGGGAGCGC CTTCCACGGC TGCAGACTCC AAATTGAGTA ACCAGGTGTC AACAATTGTG
    AGCCTGCTCT CAACACTTTG TAGAGGCTCT CCGGTAGTAA CACATGATCT CCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGACG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAGGC TTTGCCAAAG
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTCCGGA GATTGGATAG TTCTGGGGAG
    CGTTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GGGGTCAGAG GTCATCTTCT TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTCTT AAGGCATGGT GCAAACCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAAAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGACACA
    AACAAAGATG AAGAAGAATG TAATGAGCCA AAAGGAGATC CAGAAATGGC ACCCATATAC
    TTGAAAAGGT TACTGCCAGT ATTTGCACAA ACATTTCAGC AGACCATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT TATTCGAAAA ATGATTCATT TTTGTTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCGA CAGTACTAGT GGAGATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGTTGGCTCT GCAGATCATA
    AGAGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACATT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAAGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA GGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT ATGGAGTGAT
    GCGGCAGCCT TGGAATTATC CAATGGAAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GCTACAAAGA GCTCGAGGCC AAGTAAAGCC TTCTACTTCA
    AGTCAGCCTA TACTCTCAGC ACCAGGACCT ACTAAACTCA CTGTAGGGAA TTGGTCACTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCAACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTGTTT GAGTCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA GAGAGGCAGA
    AAACTAAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACCATGGC TCGAGATTTA
    TATGATGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTGATGAAG ACAGCTTTAG AAAATCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATACGAA ATGTGCAGTA GTGGCTTGGT GCAAGCACTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTAGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTC AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCTGTTGGAT TAATCCGAAA GTTAATAGCT GTGCTGGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTGCAGA TACTTACAAG AAGATTAAGA
    TTTCGGTTAG AACGTGCACC TGGTGAAACT GCACTAATAG ACAGAACTGG CAGGATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTAGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ATGATTTCGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCGT TTACTGGATT
    GGGACAAATG CCAAAACTGC ATATGAATGG GTAAATCCAG CTGCATATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ATTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCTTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTA
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AAGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGGAAG
    AATGCTAGTG GACAAACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTC
    AACGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAGGC TAATCTTAGA
    AGACAGAGAC GTCTGGTACG CTCCCAGGTA TTGAAGTACA TGGTTCCAGG GGCTCGTGTT
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACC
    GTCACAGGAG AGCTGCACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATANG TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTATCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCACTGA TAGTTCTGTT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGGATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT GACTAATAAA
    GAAGCAACTT CACAACGGCC CCTTAGCTCT TCAGCGAGTA ACAGACTCTC AGTGAGTTCT
    TTGTTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGCGCTC AGAGTTTCCC TAATTTGACC
    ACACCTGGTA CTACATCAAC AGTGACTATG TCAACGTCCA GTGTTACCAG TAGTAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTT GGTCAGTCAT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAAC CAGATGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGTCCA TCTCTGCAGC GTCGAGCTGG CTCCCGCTCT
    GATGTGACAC ACCATGCTGT CACCTCACAG CTACCACAGG TCCCTGCTGG AGCAGGGAGC
    CGACCTATTG GGGAGCAGGA AGAAGAAGAG TATGAAACTA AAGGGGGACG CCGGAGAACA
    TGGGATGATG ATTATGTGCT AAAGCGGCAG TTTTCAGCTT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GTACTAATGT ACAGCAGACA ACTGATTTAG AAATTCCGCC CCCAGGAACA
    CCTCATTCAG AGCTCTTGGA AGAAGTTGAA TGTACTCCAT CACCTCGATT AGCCCTCACT
    TTGAAAGTAA CAGGTCTTGG AACAACTCGT GAGGTTGAAT TACCACTCAC TAATTTCAGA
    TCAACCATCT TTTACTATGT ACAGAAACTG CTTCAGTTGT CCTGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCA ACATACACAA TTATGTACAG AGAAATGAAG
    GATTCTGACA AAGAAAAAGA AAATGGAAAA ATGGGTTGTT GGTCTATAGA GCATGTGGAA
    CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT
    GCAGACGCCG CTTTCCTGCG CCACTGGAAA TTAACTGGCA CTAATAAAAG TATTAGGAAA
    AACAGAAATT GTTCTCAGCT CATAGCTGCA TATAAGGACT TTTGTGAACA TGGAACTAAG
    TCTGGGTTAA ACCAGGGGGC CATTTCTACT CTTCAAAGTA GTGATATTCT TAACTTGACA
    AAAGAACAAC CTCAGGCCAA AGCAGGCAAT GGACAGAATT CTTGTGGAGT AGAAGACGTC
    CTTCAGCTTC TGCGTATTCT GTATATAGTT GCAAGTGACC CTTATTCAAG AATATCCCAA
    GAAGAAGGTG ATGAGCAGCC TCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA
    ATCACAACAA AAATTTTGCA GCAAATTGAG GAACCATTGG CATTGGCAAG TGGCGCTCTT
    CCAGACTGGT GTGAACAACT AACCAGCAAG TGTCCTTTTC TAATTCCGTT TGAAACTAGG
    CAGCTTTACT TCACATGTAC GGCGTTTGGT GCATCCAGAG CAATCGTGTG GTTACAAAAC
    CGACGTGAGG CCACTGTAGA AAGAACAAGG ACCACAAGTA GTGTAAGACG AGATGATCCT
    GGAGAGTTTC GAGTTGGTCG TCTCAAGCAT GAAAGAGTGA AAGTTCCACG TGGTGAATCA
    CTGATGGAGT GGGCTGAGAA TGTCATGCAG ATACATGCAG ATCGGAAATC AGTTCTTGAG
    GTTGAATTTT TAGGAGAAGA AGGAACTGGC TTGGGTCCCA CATTAGAGTT TTATGCTCTG
    GTGGCAGCAG AATTCCAGAG AACTGACTTA GGAGCTTGGC TTTGTGATGA TAACTTTCCA
    GATGATGAAT CTCGTCATGT TGATCTTGGA GGTGGATTGA AACCTCCTGG GTACTATGTG
    CAGCGATCAT GTGGATTGTT TACAGCTCCA TTTCCACAAG ATAGTGATGA GCTTGAAAGG
    ATCACAAAAC TCTTTCATTT CCTTGGAATT TTCTTGGCCA AATGCATTCA AGACAATAGA
    CTTGTGGACT TACCTATTTC TAAACCTTTC TTTAAACTCA TGTGTATGGG TGACATTAAA
    AGCAATATGA GCAAACTGAT TTATGAGTCA CGCGGTGATA GAGACTTACA CTGTACTGAA
    AGTCAGTCTG AAGCCTCTAC AGAAGAAGGA CATGATTCAC TCTCAGTAGG AAGCTTTGAA
    GAGGATTCAA AATCAGAGTT TATTCTAGAT CCCCCCAAAC CAAAACCCCC AGCTTGGTTT
    AATGGAATTT TAACTTGGGA AGACTTTGAA CTAGTAAACC CCCACCGAGC CAGATTTCTG
    AAAGAAATTA AAGAGCTTGC TATCAAGAGG CGCCAGATTT TAAGCAACAA AGGTCTTTCT
    GAAGATGAGA AGAACACAAA ATTACAAGAA CTAGTGCTGA AGAATCCATC AGGTTCTGGG
    CCTCCACTTA GCATAGAGGA CTTAGGTTTA AATTTCCAGT TTTGTCCTTC CTCAAGAATA
    TATGGTTTTA CAGCTGTGGA TCTCAAGCCA AGTGGTGAAG ATGAGATGGT AACAATGGAT
    AATGCAGAAG AATATGTGGA TTTGATGTTT GACTTTTGTA TGCATACAGG TATTCAGAAA
    CAGATGGAAG CCTTTAGAGA TGGATTTAAT AAAGTCTTTC CAATGGAGAA ATTAAGTTCC
    TTCAGCCATG AAGAAGTCCA AATGATTCTT TGTGGAAACC AGTCGCCATC CTGGGCAGCA
    GAGGATATAA TTAATTATAC TGAACCTAAG TTGGGTTATA CACGTGATAG CCCTGGTTTC
    CTGAGGTTCG TGAGGGTTTT ATGTGGCATG TCTTCAGATG AGAGGAAAGC ATTCCTGCAG
    TTTACCACTG GTTGTTCAAC TCTACCCCCA GGTGGACTAG CTAACTTGCA TCCGAGGCTT
    ACAGTTGTAC GCAAGGTTGA TGCTACTGAT GCAAGCTACC CATCAGTGAA TACATGTGTG
    CATTATCTAA AGTTGCCAGA ATATTCTTCG GAGGAGATCA TGAGAGAGCG CCTGCTAGCC
    GCCACAATGG AGAAGGGCTT TCATCTCAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013833590.1
    CDS257..8089
    Translation

    Target ORF information:

    RefSeq Version XM_013978136.1
    Organism Sus scrofa(Pig)
    Definition Sus scrofa HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_013978136.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGATCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAGATT
    TTTCTTGATG AAAGTGCTCC AGACAACGTG TTAGAGGTGA CCGCCCGTGC CATAACATAC
    TACCTGGACG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTGGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAATGTG TGAAGGTGTT AGAACTGATA TGCACTCGTG AGTCAGGAGC GGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACACCT GGTTCATAAA
    GATACCTTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCGTT AGCCAAGCAT GGATTAACTG AGGAATTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT ATCAGGGCCA TCATCTGCTT GCAAACCAGG TCGCAGCACC
    ACGGGAGCGC CTTCCACGGC TGCAGACTCC AAATTGAGTA ACCAGGTGTC AACAATTGTG
    AGCCTGCTCT CAACACTTTG TAGAGGCTCT CCGGTAGTAA CACATGATCT CCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGACG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAGGC TTTGCCAAAG
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTCCGGA GATTGGATAG TTCTGGGGAG
    CGTTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GGGGTCAGAG GTCATCTTCT TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTCTT AAGGCATGGT GCAAACCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAAAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGACACA
    AACAAAGATG AAGAAGAATG TAATGAGCCA AAAGGAGATC CAGAAATGGC ACCCATATAC
    TTGAAAAGGT TACTGCCAGT ATTTGCACAA ACATTTCAGC AGACCATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT TATTCGAAAA ATGATTCATT TTTGTTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCGA CAGTACTAGT GGAGATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGTTGGCTCT GCAGATCATA
    AGAGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACATT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAAGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA GGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT ATGGAGTGAT
    GCGGCAGCCT TGGAATTATC CAATGGAAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GCTACAAAGA GCTCGAGGCC AAGTAAAGCC TTCTACTTCA
    AGTCAGCCTA TACTCTCAGC ACCAGGACCT ACTAAACTCA CTGTAGGGAA TTGGTCACTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCAACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTGTTT GAGTCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA GAGAGGCAGA
    AAACTAAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACCATGGC TCGAGATTTA
    TATGATGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTGATGAAG ACAGCTTTAG AAAATCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATACGAA ATGTGCAGTA GTGGCTTGGT GCAAGCACTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTAGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTC AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCTGTTGGAT TAATCCGAAA GTTAATAGCT GTGCTGGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTGCAGA TACTTACAAG AAGATTAAGA
    TTTCGGTTAG AACGTGCACC TGGTGAAACT GCACTAATAG ACAGAACTGG CAGGATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTAGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ATGATTTCGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCGT TTACTGGATT
    GGGACAAATG CCAAAACTGC ATATGAATGG GTAAATCCAG CTGCATATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ATTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCTTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTA
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AAGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGGAAG
    AATGCTAGTG GACAAACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTC
    AACGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAGGC TAATCTTAGA
    AGACAGAGAC GTCTGGTACG CTCCCAGGTA TTGAAGTACA TGGTTCCAGG GGCTCGTGTT
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACC
    GTCACAGGAG AGCTGCACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATANG TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTATCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCACTGA TAGTTCTGTT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGGATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT GACTAATAAA
    GAAGCAACTT CACAACGGCC CCTTAGCTCT TCAGCGAGTA ACAGACTCTC AGTGAGTTCT
    TTGTTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGCGCTC AGAGTTTCCC TAATTTGACC
    ACACCTGGTA CTACATCAAC AGTGACTATG TCAACGTCCA GTGTTACCAG TAGTAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTT GGTCAGTCAT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAAC CAGATGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGTCCA TCTCTGCAGC GTCGAGCTGG CTCCCGCTCT
    GATGTGACAC ACCATGCTGT CACCTCACAG CTACCACAGG TCCCTGCTGG AGCAGGGAGC
    CGACCTATTG GGGAGCAGGA AGAAGAAGAG TATGAAACTA AAGGGGGACG CCGGAGAACA
    TGGGATGATG ATTATGTGCT AAAGCGGCAG TTTTCAGCTT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GTACTAATGT ACAGCAGACA ACTGATTTAG AAATTCCGCC CCCAGGAACA
    CCTCATTCAG AGCTCTTGGA AGAAGTTGAA TGTACTCCAT CACCTCGATT AGCCCTCACT
    TTGAAAGTAA CAGGTCTTGG AACAACTCGT GAGGTTGAAT TACCACTCAC TAATTTCAGA
    TCAACCATCT TTTACTATGT ACAGAAACTG CTTCAGTTGT CCTGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCA ACATACACAA TTATGTACAG AGAAATGAAG
    GATTCTGACA AAGAAAAAGA AAATGGAAAA ATGGGTTGTT GGTCTATAGA GCATGTGGAA
    CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT
    GCAGACGCCG CTTTCCTGCG CCACTGGAAA TTAACTGGCA CTAATAAAAG TATTAGGAAA
    AACAGAAATT GTTCTCAGCT CATAGCTGCA TATAAGGACT TTTGTGAACA TGGAACTAAG
    TCTGGGTTAA ACCAGGGGGC CATTTCTACT CTTCAAAGTA GTGATATTCT TAACTTGACA
    AAAGAACAAC CTCAGGCCAA AGCAGGCAAT GGACAGAATT CTTGTGGAGT AGAAGACGTC
    CTTCAGCTTC TGCGTATTCT GTATATAGTT GCAAGTGACC CTTATTCAAG AATATCCCAA
    GAAGAAGGTG ATGAGCAGCC TCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA
    ATCACAACAA AAATTTTGCA GCAAATTGAG GAACCATTGG CATTGGCAAG TGGCGCTCTT
    CCAGACTGGT GTGAACAACT AACCAGCAAG TGTCCTTTTC TAATTCCGTT TGAAACTAGG
    CAGCTTTACT TCACATGTAC GGCGTTTGGT GCATCCAGAG CAATCGTGTG GTTACAAAAC
    CGACGTGAGG CCACTGTAGA AAGAACAAGG ACCACAAGTA GTGTAAGACG AGATGATCCT
    GGAGAGTTTC GAGTTGGTCG TCTCAAGCAT GAAAGAGTGA AAGTTCCACG TGGTGAATCA
    CTGATGGAGT GGGCTGAGAA TGTCATGCAG ATACATGCAG ATCGGAAATC AGTTCTTGAG
    GTTGAATTTT TAGGAGAAGA AGGAACTGGC TTGGGTCCCA CATTAGAGTT TTATGCTCTG
    GTGGCAGCAG AATTCCAGAG AACTGACTTA GGAGCTTGGC TTTGTGATGA TAACTTTCCA
    GATGATGAAT CTCGTCATGT TGATCTTGGA GGTGGATTGA AACCTCCTGG GTACTATGTG
    CAGCGATCAT GTGGATTGTT TACAGCTCCA TTTCCACAAG ATAGTGATGA GCTTGAAAGG
    ATCACAAAAC TCTTTCATTT CCTTGGAATT TTCTTGGCCA AATGCATTCA AGACAATAGA
    CTTGTGGACT TACCTATTTC TAAACCTTTC TTTAAACTCA TGTGTATGGG TGACATTAAA
    AGCAATATGA GCAAACTGAT TTATGAGTCA CGCGGTGATA GAGACTTACA CTGTACTGAA
    AGTCAGTCTG AAGCCTCTAC AGAAGAAGGA CATGATTCAC TCTCAGTAGG AAGCTTTGAA
    GAGGATTCAA AATCAGAGTT TATTCTAGAT CCCCCCAAAC CAAAACCCCC AGCTTGGTTT
    AATGGAATTT TAACTTGGGA AGACTTTGAA CTAGTAAACC CCCACCGAGC CAGATTTCTG
    AAAGAAATTA AAGAGCTTGC TATCAAGAGG CGCCAGATTT TAAGCAACAA AGGTCTTTCT
    GAAGATGAGA AGAACACAAA ATTACAAGAA CTAGTGCTGA AGAATCCATC AGGTTCTGGG
    CCTCCACTTA GCATAGAGGA CTTAGGTTTA AATTTCCAGT TTTGTCCTTC CTCAAGAATA
    TATGGTTTTA CAGCTGTGGA TCTCAAGCCA AGTGGTGAAG ATGAGATGGT AACAATGGAT
    AATGCAGAAG AATATGTGGA TTTGATGTTT GACTTTTGTA TGCATACAGG TATTCAGAAA
    CAGATGGAAG CCTTTAGAGA TGGATTTAAT AAAGTCTTTC CAATGGAGAA ATTAAGTTCC
    TTCAGCCATG AAGAAGTCCA AATGATTCTT TGTGGAAACC AGTCGCCATC CTGGGCAGCA
    GAGGATATAA TTAATTATAC TGAACCTAAG TTGGGTTATA CACGTGATAG CCCTGGTTTC
    CTGAGGTTCG TGAGGGTTTT ATGTGGCATG TCTTCAGATG AGAGGAAAGC ATTCCTGCAG
    TTTACCACTG GTTGTTCAAC TCTACCCCCA GGTGGACTAG CTAACTTGCA TCCGAGGCTT
    ACAGTTGTAC GCAAGGTTGA TGCTACTGAT GCAAGCTACC CATCAGTGAA TACATGTGTG
    CATTATCTAA AGTTGCCAGA ATATTCTTCG GAGGAGATCA TGAGAGAGCG CCTGCTAGCC
    GCCACAATGG AGAAGGGCTT TCATCTCAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe223517
    Clone ID Related Accession (Same CDS sequence) XM_013978136.2
    Accession Version XM_013978136.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6420bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010449.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_013978136.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGCAGATG TGGATCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAGATT
    TTTCTTGATG AAAGTGCTCC AGACAACGTG TTAGAGGTGA CCGCCCGTGC CATAACATAC
    TACCTGGACG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTGGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAATGTG TGAAGGTGTT AGAACTGATA TGCACTCGTG AGTCAGGAGC GGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACACCT GGTTCATAAA
    GATACATTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCGTT AGCCAAGCAT GGATTAACTG AGGAATTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT ATCAGGGCCA TCATCTGCTT GCAAACCAGG TCGCAGCACC
    ACGGGAGCGC CTTCCACGGC TGCAGACTCC AAATTGAGTA ACCAGGTGTC AACAATTGTG
    AGCCTGCTCT CAACACTTTG TAGAGGCTCT CCGGTAGTAA CACATGATCT CCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGACG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAGGC TTTGCCAAAG
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTCCGGA GATTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GGGGTCAGAG GTCATCTTCT TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTCTT AAGGCATGGT GCAAACCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAAAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGACACA
    AACAAAGATG AAGAAGAATG TAATGAGCCA AAAGGAGATC CAGAAATGGC ACCCATATAC
    TTGAAAAGGT TACTGCCAGT ATTTGCACAA ACATTTCAGC AGACGATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT TATTCGAAAA ATGATTCATT TTTGTTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCGA CAGTACTAGT GGAGATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGTTGGCTCT GCAGATCATA
    AGAGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACATT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAAGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA GGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT ATGGAGTGAT
    GCGGCAGCCT TGGAATTATC CAATGGAAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GCTACAAAGA GCTCGAGGCC AAGTAAAGCC TTCTACTTCA
    AGTCAGCCTA TACTCTCAGC ACCAGGACCT ACTAAACTCA CTGTAGGGAA TTGGTCACTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCAACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTGTTT GAGTCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA GAGAGGCAGA
    AAACTAAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACCATGGC TCGAGATTTA
    TATGATGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTGATGAAG ACAGCTTTAG AAAATCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATACGAA ATGTGCAGTA GTGGCTTGGT GCAAGCACTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTAGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTC AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTGCTGGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTGCAGA TACTTACAAG AAGATTAAGA
    TTTCGGTTAG AACGTGCACC TGGTGAAACT GCACTAATAG ACAGAACTGG CAGGATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTAGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ATGATTTCGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCGT TTACTGGATT
    GGGACAAATG CCAAAACTGC ATATGAATGG GTAAATCCAG CTGCATATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ATTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCTTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTA
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AAGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGGAAG
    AATGCTAGTG GACAAACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTC
    AACGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAGGC TAATCTTAGA
    AGACAGAGAC GTCTGGTACG CTCCCAGGTA TTGAAGTACA TGGTTCCAGG GGCTCGTGTT
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACC
    GTCACAGGAG AGCTGCACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCACTGA TAGTTCTGTT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGGATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT GACTAATAAA
    GAAGCAACTT CACAACGGCC CCTTAGCTCT TCAGCGAGTA ACAGACTCTC AGTGAGTTCT
    TTGTTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGCGCTC AGAGTTTCCC TAATTTGACC
    ACACCTGGTA CTACATCAAC AGTGACTATG TCAACGTCCA GTGTTACCAG TAGTAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTT GGTCAGTCAT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAAC CAGATGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGTCCA TCTCTGCAGC GTCGAGCTGG CTCCCGCTCT
    GATGTGACAC ACCATGCTGT CACCTCACAG CTACCACAGG TCCCTGCTGG AGCAGGGAGC
    CGACCTATTG GGGAGCAGGA AGAAGAAGAG TATGAAACTA AAGGGGGACG CCGGAGAACA
    TGGGATGATG ATTATGTGCT AAAGCGGCAG TTTTCAGCTT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GTACTAATGT ACAGCAGACA ACTGATTTAG AAATTCCGCC CCCAGGAACA
    CCTCATTCAG AGCTCTTGGA AGAAGTTGAA TGTACTCCAT CACCTCGATT AGCCCTCACT
    TTGAAAGTAA CAGGTCTTGG AACAACTCGT GAGGTTGAAT TACCACTCAC TAATTTCAGA
    TCAACCATCT TTTACTATGT ACAGAAACTG CTTCAGTTGT CCTGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCA ACATACACAA TTATGTACAG AGAAATGAAG
    GATTCTGACA AAGAAAAAGA AAATGGAAAA ATGGGTTGTT GGTCTATAGA GCATGTGGAA
    CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT
    GCAGACGCCG CTTTCCTGCG CCACTGGAAA TTAACTGGCA CTAATAAAAG TATTAGGAAA
    AACAGAAATT GTTCTCAGCT CATAGCTGCA TATAAGGACT TTTGTGAACA TGGAACTAAG
    TCTGGGTTAA ACCAGGGGGC CATTTCTACT CTTCAAAGTA GTGATATTCT TAACTTGACA
    AAAGAACAAC CTCAGGCCAA AGCAGGCAAT GGACAGAATT CTTGTGGAGT AGAAGACGTC
    CTTCAGCTTC TGCGTATTCT GTATATAGTT GCAAGTGACC CTTATTCAAG AATATCCCAA
    GAAGAAGGTG ATGAGCAGCC TCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA
    ATCACAACAA AAATTTTGCA GCAAATTGAG GAACCATTGG CATTGGCAAG TGGCGCTCTT
    CCAGACTGGT GTGAACAACT AACCAGCAAG TGTCCTTTTC TAATTCCGTT TGAAACTAGG
    CAGCTTTACT TCACATGTAC GGCGTTTGGT GCATCCAGAG CAATCGTGTG GTTACAAAAC
    CGACGTGAGG CCACTGTAGA AAGAACAAGG ACCACAAGTA GTGTAAGACG AGATGATCCT
    GGAGAGTTTC GAGTTGGTCG TCTCAAGCAT GAAAGAGTGA AAGTTCCACG TGGTGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013833590.2
    CDS802..7221
    Translation

    Target ORF information:

    RefSeq Version XM_013978136.2
    Organism Sus scrofa(pig)
    Definition Sus scrofa HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_013978136.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGCAGATG TGGATCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAGATT
    TTTCTTGATG AAAGTGCTCC AGACAACGTG TTAGAGGTGA CCGCCCGTGC CATAACATAC
    TACCTGGACG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTGGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAATGTG TGAAGGTGTT AGAACTGATA TGCACTCGTG AGTCAGGAGC GGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACACCT GGTTCATAAA
    GATACATTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCGTT AGCCAAGCAT GGATTAACTG AGGAATTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT ATCAGGGCCA TCATCTGCTT GCAAACCAGG TCGCAGCACC
    ACGGGAGCGC CTTCCACGGC TGCAGACTCC AAATTGAGTA ACCAGGTGTC AACAATTGTG
    AGCCTGCTCT CAACACTTTG TAGAGGCTCT CCGGTAGTAA CACATGATCT CCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGACG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAGGC TTTGCCAAAG
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTCCGGA GATTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GGGGTCAGAG GTCATCTTCT TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTCTT AAGGCATGGT GCAAACCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAAAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGACACA
    AACAAAGATG AAGAAGAATG TAATGAGCCA AAAGGAGATC CAGAAATGGC ACCCATATAC
    TTGAAAAGGT TACTGCCAGT ATTTGCACAA ACATTTCAGC AGACGATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT TATTCGAAAA ATGATTCATT TTTGTTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCGA CAGTACTAGT GGAGATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGTTGGCTCT GCAGATCATA
    AGAGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACATT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAAGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA GGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT ATGGAGTGAT
    GCGGCAGCCT TGGAATTATC CAATGGAAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GCTACAAAGA GCTCGAGGCC AAGTAAAGCC TTCTACTTCA
    AGTCAGCCTA TACTCTCAGC ACCAGGACCT ACTAAACTCA CTGTAGGGAA TTGGTCACTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCAACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTGTTT GAGTCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA GAGAGGCAGA
    AAACTAAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACCATGGC TCGAGATTTA
    TATGATGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTGATGAAG ACAGCTTTAG AAAATCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATACGAA ATGTGCAGTA GTGGCTTGGT GCAAGCACTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTAGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTC AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTGCTGGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTGCAGA TACTTACAAG AAGATTAAGA
    TTTCGGTTAG AACGTGCACC TGGTGAAACT GCACTAATAG ACAGAACTGG CAGGATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTAGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ATGATTTCGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCGT TTACTGGATT
    GGGACAAATG CCAAAACTGC ATATGAATGG GTAAATCCAG CTGCATATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ATTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCTTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTA
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AAGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGGAAG
    AATGCTAGTG GACAAACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTC
    AACGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAGGC TAATCTTAGA
    AGACAGAGAC GTCTGGTACG CTCCCAGGTA TTGAAGTACA TGGTTCCAGG GGCTCGTGTT
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACC
    GTCACAGGAG AGCTGCACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCACTGA TAGTTCTGTT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGGATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT GACTAATAAA
    GAAGCAACTT CACAACGGCC CCTTAGCTCT TCAGCGAGTA ACAGACTCTC AGTGAGTTCT
    TTGTTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGCGCTC AGAGTTTCCC TAATTTGACC
    ACACCTGGTA CTACATCAAC AGTGACTATG TCAACGTCCA GTGTTACCAG TAGTAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTT GGTCAGTCAT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAAC CAGATGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGTCCA TCTCTGCAGC GTCGAGCTGG CTCCCGCTCT
    GATGTGACAC ACCATGCTGT CACCTCACAG CTACCACAGG TCCCTGCTGG AGCAGGGAGC
    CGACCTATTG GGGAGCAGGA AGAAGAAGAG TATGAAACTA AAGGGGGACG CCGGAGAACA
    TGGGATGATG ATTATGTGCT AAAGCGGCAG TTTTCAGCTT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GTACTAATGT ACAGCAGACA ACTGATTTAG AAATTCCGCC CCCAGGAACA
    CCTCATTCAG AGCTCTTGGA AGAAGTTGAA TGTACTCCAT CACCTCGATT AGCCCTCACT
    TTGAAAGTAA CAGGTCTTGG AACAACTCGT GAGGTTGAAT TACCACTCAC TAATTTCAGA
    TCAACCATCT TTTACTATGT ACAGAAACTG CTTCAGTTGT CCTGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCA ACATACACAA TTATGTACAG AGAAATGAAG
    GATTCTGACA AAGAAAAAGA AAATGGAAAA ATGGGTTGTT GGTCTATAGA GCATGTGGAA
    CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT
    GCAGACGCCG CTTTCCTGCG CCACTGGAAA TTAACTGGCA CTAATAAAAG TATTAGGAAA
    AACAGAAATT GTTCTCAGCT CATAGCTGCA TATAAGGACT TTTGTGAACA TGGAACTAAG
    TCTGGGTTAA ACCAGGGGGC CATTTCTACT CTTCAAAGTA GTGATATTCT TAACTTGACA
    AAAGAACAAC CTCAGGCCAA AGCAGGCAAT GGACAGAATT CTTGTGGAGT AGAAGACGTC
    CTTCAGCTTC TGCGTATTCT GTATATAGTT GCAAGTGACC CTTATTCAAG AATATCCCAA
    GAAGAAGGTG ATGAGCAGCC TCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA
    ATCACAACAA AAATTTTGCA GCAAATTGAG GAACCATTGG CATTGGCAAG TGGCGCTCTT
    CCAGACTGGT GTGAACAACT AACCAGCAAG TGTCCTTTTC TAATTCCGTT TGAAACTAGG
    CAGCTTTACT TCACATGTAC GGCGTTTGGT GCATCCAGAG CAATCGTGTG GTTACAAAAC
    CGACGTGAGG CCACTGTAGA AAGAACAAGG ACCACAAGTA GTGTAAGACG AGATGATCCT
    GGAGAGTTTC GAGTTGGTCG TCTCAAGCAT GAAAGAGTGA AAGTTCCACG TGGTGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe223518
    Clone ID Related Accession (Same CDS sequence) XM_021099877.1
    Accession Version XM_021099877.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6414bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010449.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGCAGATG TGGATCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAGATT
    TTTCTTGATG AAAGTGCTCC AGACAACGTG TTAGAGGTGA CCGCCCGTGC CATAACATAC
    TACCTGGACG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTGGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAATGTG TGAAGGTGTT AGAACTGATA TGCACTCGTG AGTCAGGAGC GGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACACCT GGTTCATAAA
    GATACATTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCGTT AGCCAAGCAT GGATTAACTG AGGAATTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT ATCAGGGCCA TCATCTGCTT GCAAACCAGG TCGCAGCACC
    ACGGGAGCGC CTTCCACGGC TGCAGACTCC AAATTGAGTA ACCAGGTGTC AACAATTGTG
    AGCCTGCTCT CAACACTTTG TAGAGGCTCT CCGGTAGTAA CACATGATCT CCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGACG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAGGC TTTGCCAAAG
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTCCGGA GATTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GGGGTCAGAG GTCATCTTCT TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTCTT AAGGCATGGT GCAAACCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAAAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGACACA
    AACAAAGATG AAGAAGAATG TAATGAGCCA AAAGGAGATC CAGAAATGGC ACCCATATAC
    TTGAAAAGGT TACTGCCAGT ATTTGCACAA ACATTTCAGC AGACGATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT TATTCGAAAA ATGATTCATT TTTGTTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCGA CAGTACTAGT GGAGATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGTTGGCTCT GCAGATCATA
    AGAGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACATT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAAGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA GGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT ATGGAGTGAT
    GCGGCAGCCT TGGAATTATC CAATGGAAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GCTACAAAGA GCTCGAGGCC AAGTAAAGCC TTCTACTTCA
    AGTCAGCCTA TACTCTCAGC ACCAGGACCT ACTAAACTCA CTGTAGGGAA TTGGTCACTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCAACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTGTTT GAGTCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA GAGAGGCAGA
    AAACTAAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACCATGGC TCGAGATTTA
    TATGATGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTGATGAAG ACAGCTTTAG AAAATCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATACGAA ATGTGCAGTA GTGGCTTGGT GCAAGCACTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTAGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTC AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTGCTGGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTGCAGA TACTTACAAG AAGATTAAGA
    TTTCGGTTAG AACGTGCACC TGGTGAAACT GCACTAATAG ACAGAACTGG CAGGATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTAGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ATGATTTCGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCGT TTACTGGATT
    GGGACAAATG CCAAAACTGC ATATGAATGG GTAAATCCAG CTGCATATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ATTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCTTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTA
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AAGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGGAAG
    AATGCTAGTG GACAAACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTC
    AACGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAGGC TAATCTTAGA
    AGACAGAGAC GTCTGGTACG CTCCCAGGTA TTGAAGTACA TGGTTCCAGG GGCTCGTGTT
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACC
    GTCACAGGAG AGCTGCACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCACTGA TAGTTCTGTT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGGATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT GACTAATAAA
    GAAGCAACTT CACAACGGCC CCTTAGCTCT TCAGCGAGTA ACAGACTCTC AGTGAGTTCT
    TTGTTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGCGCTC AGAGTTTCCC TAATTTGACC
    ACACCTGGTA CTACATCAAC AGTGACTATG TCAACGTCCA GTGTTACCAG TAGTAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTT GGTCAGTCAT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAAC CAGATGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGA TTCTGAGACG TCCATCTCTG CAGCGTCGAG CTGGCTCCCG CTCTGATGTG
    ACACACCATG CTGTCACCTC ACAGCTACCA CAGGTCCCTG CTGGAGCAGG GAGCCGACCT
    ATTGGGGAGC AGGAAGAAGA AGAGTATGAA ACTAAAGGGG GACGCCGGAG AACATGGGAT
    GATGATTATG TGCTAAAGCG GCAGTTTTCA GCTTTGGTTC CTGCTTTTGA TCCTAGACCT
    GGTCGTACTA ATGTACAGCA GACAACTGAT TTAGAAATTC CGCCCCCAGG AACACCTCAT
    TCAGAGCTCT TGGAAGAAGT TGAATGTACT CCATCACCTC GATTAGCCCT CACTTTGAAA
    GTAACAGGTC TTGGAACAAC TCGTGAGGTT GAATTACCAC TCACTAATTT CAGATCAACC
    ATCTTTTACT ATGTACAGAA ACTGCTTCAG TTGTCCTGTA ATGGCAATGT GAAATCAGAT
    AAACTTAGGC GTATTTGGGA GCCAACATAC ACAATTATGT ACAGAGAAAT GAAGGATTCT
    GACAAAGAAA AAGAAAATGG AAAAATGGGT TGTTGGTCTA TAGAGCATGT GGAACAGTAC
    CTTGGCACTG ATGAATTACC AAAGAATGAC TTGATAACCT ACCTGCAGAA GAATGCAGAC
    GCCGCTTTCC TGCGCCACTG GAAATTAACT GGCACTAATA AAAGTATTAG GAAAAACAGA
    AATTGTTCTC AGCTCATAGC TGCATATAAG GACTTTTGTG AACATGGAAC TAAGTCTGGG
    TTAAACCAGG GGGCCATTTC TACTCTTCAA AGTAGTGATA TTCTTAACTT GACAAAAGAA
    CAACCTCAGG CCAAAGCAGG CAATGGACAG AATTCTTGTG GAGTAGAAGA CGTCCTTCAG
    CTTCTGCGTA TTCTGTATAT AGTTGCAAGT GACCCTTATT CAAGAATATC CCAAGAAGAA
    GGTGATGAGC AGCCTCAGTT TACTTTTCCA CCAGATGAAT TCACTAGCAA AAAAATCACA
    ACAAAAATTT TGCAGCAAAT TGAGGAACCA TTGGCATTGG CAAGTGGCGC TCTTCCAGAC
    TGGTGTGAAC AACTAACCAG CAAGTGTCCT TTTCTAATTC CGTTTGAAAC TAGGCAGCTT
    TACTTCACAT GTACGGCGTT TGGTGCATCC AGAGCAATCG TGTGGTTACA AAACCGACGT
    GAGGCCACTG TAGAAAGAAC AAGGACCACA AGTAGTGTAA GACGAGATGA TCCTGGAGAG
    TTTCGAGTTG GTCGTCTCAA GCATGAAAGA GTGAAAGTTC CACGTGGTGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020955536.1
    CDS802..7215
    Translation

    Target ORF information:

    RefSeq Version XM_021099877.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021099877.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGCAGATG TGGATCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAGATT
    TTTCTTGATG AAAGTGCTCC AGACAACGTG TTAGAGGTGA CCGCCCGTGC CATAACATAC
    TACCTGGACG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTGGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAATGTG TGAAGGTGTT AGAACTGATA TGCACTCGTG AGTCAGGAGC GGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACACCT GGTTCATAAA
    GATACATTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCGTT AGCCAAGCAT GGATTAACTG AGGAATTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT ATCAGGGCCA TCATCTGCTT GCAAACCAGG TCGCAGCACC
    ACGGGAGCGC CTTCCACGGC TGCAGACTCC AAATTGAGTA ACCAGGTGTC AACAATTGTG
    AGCCTGCTCT CAACACTTTG TAGAGGCTCT CCGGTAGTAA CACATGATCT CCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGACG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAGGC TTTGCCAAAG
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTCCGGA GATTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GGGGTCAGAG GTCATCTTCT TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTCTT AAGGCATGGT GCAAACCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAAAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGACACA
    AACAAAGATG AAGAAGAATG TAATGAGCCA AAAGGAGATC CAGAAATGGC ACCCATATAC
    TTGAAAAGGT TACTGCCAGT ATTTGCACAA ACATTTCAGC AGACGATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT TATTCGAAAA ATGATTCATT TTTGTTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCGA CAGTACTAGT GGAGATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGTTGGCTCT GCAGATCATA
    AGAGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACATT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAAGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA GGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT ATGGAGTGAT
    GCGGCAGCCT TGGAATTATC CAATGGAAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GCTACAAAGA GCTCGAGGCC AAGTAAAGCC TTCTACTTCA
    AGTCAGCCTA TACTCTCAGC ACCAGGACCT ACTAAACTCA CTGTAGGGAA TTGGTCACTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCAACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTGTTT GAGTCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA GAGAGGCAGA
    AAACTAAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACCATGGC TCGAGATTTA
    TATGATGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTGATGAAG ACAGCTTTAG AAAATCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATACGAA ATGTGCAGTA GTGGCTTGGT GCAAGCACTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTAGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTC AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTGCTGGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTGCAGA TACTTACAAG AAGATTAAGA
    TTTCGGTTAG AACGTGCACC TGGTGAAACT GCACTAATAG ACAGAACTGG CAGGATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTAGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ATGATTTCGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCGT TTACTGGATT
    GGGACAAATG CCAAAACTGC ATATGAATGG GTAAATCCAG CTGCATATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ATTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCTTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTA
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AAGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGGAAG
    AATGCTAGTG GACAAACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTC
    AACGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAGGC TAATCTTAGA
    AGACAGAGAC GTCTGGTACG CTCCCAGGTA TTGAAGTACA TGGTTCCAGG GGCTCGTGTT
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACC
    GTCACAGGAG AGCTGCACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCACTGA TAGTTCTGTT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGGATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT GACTAATAAA
    GAAGCAACTT CACAACGGCC CCTTAGCTCT TCAGCGAGTA ACAGACTCTC AGTGAGTTCT
    TTGTTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGCGCTC AGAGTTTCCC TAATTTGACC
    ACACCTGGTA CTACATCAAC AGTGACTATG TCAACGTCCA GTGTTACCAG TAGTAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTT GGTCAGTCAT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAAC CAGATGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGA TTCTGAGACG TCCATCTCTG CAGCGTCGAG CTGGCTCCCG CTCTGATGTG
    ACACACCATG CTGTCACCTC ACAGCTACCA CAGGTCCCTG CTGGAGCAGG GAGCCGACCT
    ATTGGGGAGC AGGAAGAAGA AGAGTATGAA ACTAAAGGGG GACGCCGGAG AACATGGGAT
    GATGATTATG TGCTAAAGCG GCAGTTTTCA GCTTTGGTTC CTGCTTTTGA TCCTAGACCT
    GGTCGTACTA ATGTACAGCA GACAACTGAT TTAGAAATTC CGCCCCCAGG AACACCTCAT
    TCAGAGCTCT TGGAAGAAGT TGAATGTACT CCATCACCTC GATTAGCCCT CACTTTGAAA
    GTAACAGGTC TTGGAACAAC TCGTGAGGTT GAATTACCAC TCACTAATTT CAGATCAACC
    ATCTTTTACT ATGTACAGAA ACTGCTTCAG TTGTCCTGTA ATGGCAATGT GAAATCAGAT
    AAACTTAGGC GTATTTGGGA GCCAACATAC ACAATTATGT ACAGAGAAAT GAAGGATTCT
    GACAAAGAAA AAGAAAATGG AAAAATGGGT TGTTGGTCTA TAGAGCATGT GGAACAGTAC
    CTTGGCACTG ATGAATTACC AAAGAATGAC TTGATAACCT ACCTGCAGAA GAATGCAGAC
    GCCGCTTTCC TGCGCCACTG GAAATTAACT GGCACTAATA AAAGTATTAG GAAAAACAGA
    AATTGTTCTC AGCTCATAGC TGCATATAAG GACTTTTGTG AACATGGAAC TAAGTCTGGG
    TTAAACCAGG GGGCCATTTC TACTCTTCAA AGTAGTGATA TTCTTAACTT GACAAAAGAA
    CAACCTCAGG CCAAAGCAGG CAATGGACAG AATTCTTGTG GAGTAGAAGA CGTCCTTCAG
    CTTCTGCGTA TTCTGTATAT AGTTGCAAGT GACCCTTATT CAAGAATATC CCAAGAAGAA
    GGTGATGAGC AGCCTCAGTT TACTTTTCCA CCAGATGAAT TCACTAGCAA AAAAATCACA
    ACAAAAATTT TGCAGCAAAT TGAGGAACCA TTGGCATTGG CAAGTGGCGC TCTTCCAGAC
    TGGTGTGAAC AACTAACCAG CAAGTGTCCT TTTCTAATTC CGTTTGAAAC TAGGCAGCTT
    TACTTCACAT GTACGGCGTT TGGTGCATCC AGAGCAATCG TGTGGTTACA AAACCGACGT
    GAGGCCACTG TAGAAAGAAC AAGGACCACA AGTAGTGTAA GACGAGATGA TCCTGGAGAG
    TTTCGAGTTG GTCGTCTCAA GCATGAAAGA GTGAAAGTTC CACGTGGTGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe223519
    Clone ID Related Accession (Same CDS sequence) XM_021099878.1
    Accession Version XM_021099878.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6279bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010449.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCAGATG TGGATCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAGATT
    TTTCTTGATG AAAGTGCTCC AGACAACGTG TTAGAGGTGA CCGCCCGTGC CATAACATAC
    TACCTGGACG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTGGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAATGTG TGAAGGTGTT AGAACTGATA TGCACTCGTG AGTCAGGAGC GGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACACCT GGTTCATAAA
    GATACATTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCGTT AGCCAAGCAT GGATTAACTG AGGAATTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT ATCAGGGCCA TCATCTGCTT GCAAACCAGG TCGCAGCACC
    ACGGGAGCGC CTTCCACGGC TGCAGACTCC AAATTGAGTA ACCAGGTGTC AACAATTGTG
    AGCCTGCTCT CAACACTTTG TAGAGGCTCT CCGGTAGTAA CACATGATCT CCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGACG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAGGC TTTGCCAAAG
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTCCGGA GATTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GGGGTCAGAG GTCATCTTCT TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTCTT AAGGCATGGT GCAAACCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAAAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGACACA
    AACAAAGATG AAGAAGAATG TAATGAGCCA AAAGGAGATC CAGAAATGGC ACCCATATAC
    TTGAAAAGGT TACTGCCAGT ATTTGCACAA ACATTTCAGC AGACGATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT TATTCGAAAA ATGATTCATT TTTGTTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCGA CAGTACTAGT GGAGATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGTTGGCTCT GCAGATCATA
    AGAGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACATT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAAGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA GGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT ATGGAGTGAT
    GCGGCAGCCT TGGAATTATC CAATGGAAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GCTACAAAGA GCTCGAGGCC AAGTAAAGCC TTCTACTTCA
    AGTCAGCCTA TACTCTCAGC ACCAGGACCT ACTAAACTCA CTGTAGGGAA TTGGTCACTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCAACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTGTTT GAGTCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA GAGAGGCAGA
    AAACTAAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACCATGGC TCGAGATTTA
    TATGATGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTGATGAAG ACAGCTTTAG AAAATCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATACGAA ATGTGCAGTA GTGGCTTGGT GCAAGCACTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTAGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTC AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTGCTGGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTGCAGA TACTTACAAG AAGATTAAGA
    TTTCGGTTAG AACGTGCACC TGGTGAAACT GCACTAATAG ACAGAACTGG CAGGATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTAGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ATGATTTCGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCGT TTACTGGATT
    GGGACAAATG CCAAAACTGC ATATGAATGG GTAAATCCAG CTGCATATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ATTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCTTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTA
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AAGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGGAAG
    AATGCTAGTG GACAAACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTC
    AACGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAGGC TAATCTTAGA
    AGACAGAGAC GTCTGGTACG CTCCCAGGTA TTGAAGTACA TGGTTCCAGG GGCTCGTGTT
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACC
    GTCACAGGAG AGCTGCACAA TGGCACAACG CAATCATGGA GCAGCTTGGT GAAAAACAAC
    TGTCCAGACA AGACATCTGC TGCTGCAGGC TCCTCAAGTA GAAAAGGAAG CAGCAGTTCT
    GTGTGTAGCG TGGCCAGTAG CAGCGACATC AGCTTGGGTT CGACCAAAAC GGAACGGAGA
    TCAGAAATTG TAATGGAACA CAGTATAGTT TCAGGAGCTG ATGTCCATGA ACCAATTGTT
    GTTCTTTCAT CTGCTGAAAA CGTCCCTCAA ACAGAAGTAG GGTCATCTTC CAGTGCAAGT
    ACCAGCACCT TAACAGCGGA AACGGGAAGT GAAAATGCTG AAAGAAAGTT AGGCACTGAT
    AGTTCTGTTC GTACTCCTGG GGAGTCTAGT GCAATATCCA TGGGGATTGT TAGTGTTAGT
    TCTCCTGATG TTAGTTCAGT ATCTGAGTTG ACTAATAAAG AAGCAACTTC ACAACGGCCC
    CTTAGCTCTT CAGCGAGTAA CAGACTCTCA GTGAGTTCTT TGTTGGCTGC TGGGGCCCCT
    ATGAGCTCTA GTGCAAGTGT ACCTAACCTG TCCTCAAGAG AAACATCTAG CTTGGAGAGT
    TTTGTAAGGA GAGTGGCAAA CATAGCACGG ACTAATGCCA CGAACAACAT GAATCTAAGC
    CGAAGCAGCA GTGATAACAA CACTAATACT TTGGGGAGGA ATGTGATGAG CACAGCAACT
    TCTCCTCTTA TGGGCGCTCA GAGTTTCCCT AATTTGACCA CACCTGGTAC TACATCAACA
    GTGACTATGT CAACGTCCAG TGTTACCAGT AGTAGCAATG TAGCTACAGC AACAACAGTT
    TTATCAGTTG GTCAGTCATT AAGTAATACT TTAACCACCA GCCTCACATC AACCTCCAGT
    GAGAGTGACA CAGGTCAGGA AGCAGAATAT TCCTTATATG ATTTCCTTGA TAGCTGCCGT
    GCCAGTACTC TGTTAGCTGA GCTAGATGAC GATGAGGACT TGCCAGAACC AGATGAGGAA
    GATGATGAAA ATGAAGATGA CAATCAGGAG GACCAAGAAT ACGAGGAGGT TATGATTCTG
    AGACGTCCAT CTCTGCAGCG TCGAGCTGGC TCCCGCTCTG ATGTGACACA CCATGCTGTC
    ACCTCACAGC TACCACAGGT CCCTGCTGGA GCAGGGAGCC GACCTATTGG GGAGCAGGAA
    GAAGAAGAGT ATGAAACTAA AGGGGGACGC CGGAGAACAT GGGATGATGA TTATGTGCTA
    AAGCGGCAGT TTTCAGCTTT GGTTCCTGCT TTTGATCCTA GACCTGGTCG TACTAATGTA
    CAGCAGACAA CTGATTTAGA AATTCCGCCC CCAGGAACAC CTCATTCAGA GCTCTTGGAA
    GAAGTTGAAT GTACTCCATC ACCTCGATTA GCCCTCACTT TGAAAGTAAC AGGTCTTGGA
    ACAACTCGTG AGGTTGAATT ACCACTCACT AATTTCAGAT CAACCATCTT TTACTATGTA
    CAGAAACTGC TTCAGTTGTC CTGTAATGGC AATGTGAAAT CAGATAAACT TAGGCGTATT
    TGGGAGCCAA CATACACAAT TATGTACAGA GAAATGAAGG ATTCTGACAA AGAAAAAGAA
    AATGGAAAAA TGGGTTGTTG GTCTATAGAG CATGTGGAAC AGTACCTTGG CACTGATGAA
    TTACCAAAGA ATGACTTGAT AACCTACCTG CAGAAGAATG CAGACGCCGC TTTCCTGCGC
    CACTGGAAAT TAACTGGCAC TAATAAAAGT ATTAGGAAAA ACAGAAATTG TTCTCAGCTC
    ATAGCTGCAT ATAAGGACTT TTGTGAACAT GGAACTAAGT CTGGGTTAAA CCAGGGGGCC
    ATTTCTACTC TTCAAAGTAG TGATATTCTT AACTTGACAA AAGAACAACC TCAGGCCAAA
    GCAGGCAATG GACAGAATTC TTGTGGAGTA GAAGACGTCC TTCAGCTTCT GCGTATTCTG
    TATATAGTTG CAAGTGACCC TTATTCAAGA ATATCCCAAG AAGAAGGTGA TGAGCAGCCT
    CAGTTTACTT TTCCACCAGA TGAATTCACT AGCAAAAAAA TCACAACAAA AATTTTGCAG
    CAAATTGAGG AACCATTGGC ATTGGCAAGT GGCGCTCTTC CAGACTGGTG TGAACAACTA
    ACCAGCAAGT GTCCTTTTCT AATTCCGTTT GAAACTAGGC AGCTTTACTT CACATGTACG
    GCGTTTGGTG CATCCAGAGC AATCGTGTGG TTACAAAACC GACGTGAGGC CACTGTAGAA
    AGAACAAGGA CCACAAGTAG TGTAAGACGA GATGATCCTG GAGAGTTTCG AGTTGGTCGT
    CTCAAGCATG AAAGAGTGAA AGTTCCACGT GGTGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020955537.1
    CDS802..7080
    Translation

    Target ORF information:

    RefSeq Version XM_021099878.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021099878.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCAGATG TGGATCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAGATT
    TTTCTTGATG AAAGTGCTCC AGACAACGTG TTAGAGGTGA CCGCCCGTGC CATAACATAC
    TACCTGGACG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTGGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAATGTG TGAAGGTGTT AGAACTGATA TGCACTCGTG AGTCAGGAGC GGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACACCT GGTTCATAAA
    GATACATTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCGTT AGCCAAGCAT GGATTAACTG AGGAATTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT ATCAGGGCCA TCATCTGCTT GCAAACCAGG TCGCAGCACC
    ACGGGAGCGC CTTCCACGGC TGCAGACTCC AAATTGAGTA ACCAGGTGTC AACAATTGTG
    AGCCTGCTCT CAACACTTTG TAGAGGCTCT CCGGTAGTAA CACATGATCT CCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGACG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAGGC TTTGCCAAAG
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTCCGGA GATTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GGGGTCAGAG GTCATCTTCT TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTCTT AAGGCATGGT GCAAACCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAAAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGACACA
    AACAAAGATG AAGAAGAATG TAATGAGCCA AAAGGAGATC CAGAAATGGC ACCCATATAC
    TTGAAAAGGT TACTGCCAGT ATTTGCACAA ACATTTCAGC AGACGATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT TATTCGAAAA ATGATTCATT TTTGTTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCGA CAGTACTAGT GGAGATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGTTGGCTCT GCAGATCATA
    AGAGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACATT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAAGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA GGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT ATGGAGTGAT
    GCGGCAGCCT TGGAATTATC CAATGGAAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GCTACAAAGA GCTCGAGGCC AAGTAAAGCC TTCTACTTCA
    AGTCAGCCTA TACTCTCAGC ACCAGGACCT ACTAAACTCA CTGTAGGGAA TTGGTCACTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCAACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTGTTT GAGTCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA GAGAGGCAGA
    AAACTAAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACCATGGC TCGAGATTTA
    TATGATGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTGATGAAG ACAGCTTTAG AAAATCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATACGAA ATGTGCAGTA GTGGCTTGGT GCAAGCACTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTAGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTC AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTGCTGGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTGCAGA TACTTACAAG AAGATTAAGA
    TTTCGGTTAG AACGTGCACC TGGTGAAACT GCACTAATAG ACAGAACTGG CAGGATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTAGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ATGATTTCGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCGT TTACTGGATT
    GGGACAAATG CCAAAACTGC ATATGAATGG GTAAATCCAG CTGCATATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ATTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCTTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTA
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AAGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGGAAG
    AATGCTAGTG GACAAACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTC
    AACGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAGGC TAATCTTAGA
    AGACAGAGAC GTCTGGTACG CTCCCAGGTA TTGAAGTACA TGGTTCCAGG GGCTCGTGTT
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACC
    GTCACAGGAG AGCTGCACAA TGGCACAACG CAATCATGGA GCAGCTTGGT GAAAAACAAC
    TGTCCAGACA AGACATCTGC TGCTGCAGGC TCCTCAAGTA GAAAAGGAAG CAGCAGTTCT
    GTGTGTAGCG TGGCCAGTAG CAGCGACATC AGCTTGGGTT CGACCAAAAC GGAACGGAGA
    TCAGAAATTG TAATGGAACA CAGTATAGTT TCAGGAGCTG ATGTCCATGA ACCAATTGTT
    GTTCTTTCAT CTGCTGAAAA CGTCCCTCAA ACAGAAGTAG GGTCATCTTC CAGTGCAAGT
    ACCAGCACCT TAACAGCGGA AACGGGAAGT GAAAATGCTG AAAGAAAGTT AGGCACTGAT
    AGTTCTGTTC GTACTCCTGG GGAGTCTAGT GCAATATCCA TGGGGATTGT TAGTGTTAGT
    TCTCCTGATG TTAGTTCAGT ATCTGAGTTG ACTAATAAAG AAGCAACTTC ACAACGGCCC
    CTTAGCTCTT CAGCGAGTAA CAGACTCTCA GTGAGTTCTT TGTTGGCTGC TGGGGCCCCT
    ATGAGCTCTA GTGCAAGTGT ACCTAACCTG TCCTCAAGAG AAACATCTAG CTTGGAGAGT
    TTTGTAAGGA GAGTGGCAAA CATAGCACGG ACTAATGCCA CGAACAACAT GAATCTAAGC
    CGAAGCAGCA GTGATAACAA CACTAATACT TTGGGGAGGA ATGTGATGAG CACAGCAACT
    TCTCCTCTTA TGGGCGCTCA GAGTTTCCCT AATTTGACCA CACCTGGTAC TACATCAACA
    GTGACTATGT CAACGTCCAG TGTTACCAGT AGTAGCAATG TAGCTACAGC AACAACAGTT
    TTATCAGTTG GTCAGTCATT AAGTAATACT TTAACCACCA GCCTCACATC AACCTCCAGT
    GAGAGTGACA CAGGTCAGGA AGCAGAATAT TCCTTATATG ATTTCCTTGA TAGCTGCCGT
    GCCAGTACTC TGTTAGCTGA GCTAGATGAC GATGAGGACT TGCCAGAACC AGATGAGGAA
    GATGATGAAA ATGAAGATGA CAATCAGGAG GACCAAGAAT ACGAGGAGGT TATGATTCTG
    AGACGTCCAT CTCTGCAGCG TCGAGCTGGC TCCCGCTCTG ATGTGACACA CCATGCTGTC
    ACCTCACAGC TACCACAGGT CCCTGCTGGA GCAGGGAGCC GACCTATTGG GGAGCAGGAA
    GAAGAAGAGT ATGAAACTAA AGGGGGACGC CGGAGAACAT GGGATGATGA TTATGTGCTA
    AAGCGGCAGT TTTCAGCTTT GGTTCCTGCT TTTGATCCTA GACCTGGTCG TACTAATGTA
    CAGCAGACAA CTGATTTAGA AATTCCGCCC CCAGGAACAC CTCATTCAGA GCTCTTGGAA
    GAAGTTGAAT GTACTCCATC ACCTCGATTA GCCCTCACTT TGAAAGTAAC AGGTCTTGGA
    ACAACTCGTG AGGTTGAATT ACCACTCACT AATTTCAGAT CAACCATCTT TTACTATGTA
    CAGAAACTGC TTCAGTTGTC CTGTAATGGC AATGTGAAAT CAGATAAACT TAGGCGTATT
    TGGGAGCCAA CATACACAAT TATGTACAGA GAAATGAAGG ATTCTGACAA AGAAAAAGAA
    AATGGAAAAA TGGGTTGTTG GTCTATAGAG CATGTGGAAC AGTACCTTGG CACTGATGAA
    TTACCAAAGA ATGACTTGAT AACCTACCTG CAGAAGAATG CAGACGCCGC TTTCCTGCGC
    CACTGGAAAT TAACTGGCAC TAATAAAAGT ATTAGGAAAA ACAGAAATTG TTCTCAGCTC
    ATAGCTGCAT ATAAGGACTT TTGTGAACAT GGAACTAAGT CTGGGTTAAA CCAGGGGGCC
    ATTTCTACTC TTCAAAGTAG TGATATTCTT AACTTGACAA AAGAACAACC TCAGGCCAAA
    GCAGGCAATG GACAGAATTC TTGTGGAGTA GAAGACGTCC TTCAGCTTCT GCGTATTCTG
    TATATAGTTG CAAGTGACCC TTATTCAAGA ATATCCCAAG AAGAAGGTGA TGAGCAGCCT
    CAGTTTACTT TTCCACCAGA TGAATTCACT AGCAAAAAAA TCACAACAAA AATTTTGCAG
    CAAATTGAGG AACCATTGGC ATTGGCAAGT GGCGCTCTTC CAGACTGGTG TGAACAACTA
    ACCAGCAAGT GTCCTTTTCT AATTCCGTTT GAAACTAGGC AGCTTTACTT CACATGTACG
    GCGTTTGGTG CATCCAGAGC AATCGTGTGG TTACAAAACC GACGTGAGGC CACTGTAGAA
    AGAACAAGGA CCACAAGTAG TGTAAGACGA GATGATCCTG GAGAGTTTCG AGTTGGTCGT
    CTCAAGCATG AAAGAGTGAA AGTTCCACGT GGTGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe223520
    Clone ID Related Accession (Same CDS sequence) XM_021099879.1
    Accession Version XM_021099879.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6273bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010449.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCAGATG TGGATCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAGATT
    TTTCTTGATG AAAGTGCTCC AGACAACGTG TTAGAGGTGA CCGCCCGTGC CATAACATAC
    TACCTGGACG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTGGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAATGTG TGAAGGTGTT AGAACTGATA TGCACTCGTG AGTCAGGAGC GGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACACCT GGTTCATAAA
    GATACATTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCGTT AGCCAAGCAT GGATTAACTG AGGAATTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT ATCAGGGCCA TCATCTGCTT GCAAACCAGG TCGCAGCACC
    ACGGGAGCGC CTTCCACGGC TGCAGACTCC AAATTGAGTA ACCAGGTGTC AACAATTGTG
    AGCCTGCTCT CAACACTTTG TAGAGGCTCT CCGGTAGTAA CACATGATCT CCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGACG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAGGC TTTGCCAAAG
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTCCGGA GATTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GGGGTCAGAG GTCATCTTCT TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTCTT AAGGCATGGT GCAAACCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAAAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGACACA
    AACAAAGATG AAGAAGAATG TAATGAGCCA AAAGGAGATC CAGAAATGGC ACCCATATAC
    TTGAAAAGGT TACTGCCAGT ATTTGCACAA ACATTTCAGC AGACGATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT TATTCGAAAA ATGATTCATT TTTGTTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCGA CAGTACTAGT GGAGATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGTTGGCTCT GCAGATCATA
    AGAGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACATT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAAGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA GGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT ATGGAGTGAT
    GCGGCAGCCT TGGAATTATC CAATGGAAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GCTACAAAGA GCTCGAGGCC AAGTAAAGCC TTCTACTTCA
    AGTCAGCCTA TACTCTCAGC ACCAGGACCT ACTAAACTCA CTGTAGGGAA TTGGTCACTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCAACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTGTTT GAGTCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA GAGAGGCAGA
    AAACTAAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACCATGGC TCGAGATTTA
    TATGATGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTGATGAAG ACAGCTTTAG AAAATCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATACGAA ATGTGCAGTA GTGGCTTGGT GCAAGCACTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTAGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTC AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTGCTGGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTGCAGA TACTTACAAG AAGATTAAGA
    TTTCGGTTAG AACGTGCACC TGGTGAAACT GCACTAATAG ACAGAACTGG CAGGATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTAGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ATGATTTCGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCGT TTACTGGATT
    GGGACAAATG CCAAAACTGC ATATGAATGG GTAAATCCAG CTGCATATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ATTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCTTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTA
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AAGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGGAAG
    AATGCTAGTG GACAAACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTC
    AACGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAGGC TAATCTTAGA
    AGACAGAGAC GTCTGGTACG CTCCCAGGTA TTGAAGTACA TGGTTCCAGG GGCTCGTGTT
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACC
    GTCACAGGAG AGCTGCACAA TGGCACAACG CAATCATGGA GCAGCTTGGT GAAAAACAAC
    TGTCCAGACA AGACATCTGC TGCTGCAGGC TCCTCAAGTA GAAAAGGAAG CAGCAGTTCT
    GTGTGTAGCG TGGCCAGTAG CAGCGACATC AGCTTGGGTT CGACCAAAAC GGAACGGAGA
    TCAGAAATTG TAATGGAACA CAGTATAGTT TCAGGAGCTG ATGTCCATGA ACCAATTGTT
    GTTCTTTCAT CTGCTGAAAA CGTCCCTCAA ACAGAAGTAG GGTCATCTTC CAGTGCAAGT
    ACCAGCACCT TAACAGCGGA AACGGGAAGT GAAAATGCTG AAAGAAAGTT AGGCACTGAT
    AGTTCTGTTC GTACTCCTGG GGAGTCTAGT GCAATATCCA TGGGGATTGT TAGTGTTAGT
    TCTCCTGATG TTAGTTCAGT ATCTGAGTTG ACTAATAAAG AAGCAACTTC ACAACGGCCC
    CTTAGCTCTT CAGCGAGTAA CAGACTCTCA GTGAGTTCTT TGTTGGCTGC TGGGGCCCCT
    ATGAGCTCTA GTGCAAGTGT ACCTAACCTG TCCTCAAGAG AAACATCTAG CTTGGAGAGT
    TTTGTAAGGA GAGTGGCAAA CATAGCACGG ACTAATGCCA CGAACAACAT GAATCTAAGC
    CGAAGCAGCA GTGATAACAA CACTAATACT TTGGGGAGGA ATGTGATGAG CACAGCAACT
    TCTCCTCTTA TGGGCGCTCA GAGTTTCCCT AATTTGACCA CACCTGGTAC TACATCAACA
    GTGACTATGT CAACGTCCAG TGTTACCAGT AGTAGCAATG TAGCTACAGC AACAACAGTT
    TTATCAGTTG GTCAGTCATT AAGTAATACT TTAACCACCA GCCTCACATC AACCTCCAGT
    GAGAGTGACA CAGGTCAGGA AGCAGAATAT TCCTTATATG ATTTCCTTGA TAGCTGCCGT
    GCCAGTACTC TGTTAGCTGA GCTAGATGAC GATGAGGACT TGCCAGAACC AGATGAGGAA
    GATGATGAAA ATGAAGATGA CAATCAGGAG GACCAAGAAT ACGAGGAGAT TCTGAGACGT
    CCATCTCTGC AGCGTCGAGC TGGCTCCCGC TCTGATGTGA CACACCATGC TGTCACCTCA
    CAGCTACCAC AGGTCCCTGC TGGAGCAGGG AGCCGACCTA TTGGGGAGCA GGAAGAAGAA
    GAGTATGAAA CTAAAGGGGG ACGCCGGAGA ACATGGGATG ATGATTATGT GCTAAAGCGG
    CAGTTTTCAG CTTTGGTTCC TGCTTTTGAT CCTAGACCTG GTCGTACTAA TGTACAGCAG
    ACAACTGATT TAGAAATTCC GCCCCCAGGA ACACCTCATT CAGAGCTCTT GGAAGAAGTT
    GAATGTACTC CATCACCTCG ATTAGCCCTC ACTTTGAAAG TAACAGGTCT TGGAACAACT
    CGTGAGGTTG AATTACCACT CACTAATTTC AGATCAACCA TCTTTTACTA TGTACAGAAA
    CTGCTTCAGT TGTCCTGTAA TGGCAATGTG AAATCAGATA AACTTAGGCG TATTTGGGAG
    CCAACATACA CAATTATGTA CAGAGAAATG AAGGATTCTG ACAAAGAAAA AGAAAATGGA
    AAAATGGGTT GTTGGTCTAT AGAGCATGTG GAACAGTACC TTGGCACTGA TGAATTACCA
    AAGAATGACT TGATAACCTA CCTGCAGAAG AATGCAGACG CCGCTTTCCT GCGCCACTGG
    AAATTAACTG GCACTAATAA AAGTATTAGG AAAAACAGAA ATTGTTCTCA GCTCATAGCT
    GCATATAAGG ACTTTTGTGA ACATGGAACT AAGTCTGGGT TAAACCAGGG GGCCATTTCT
    ACTCTTCAAA GTAGTGATAT TCTTAACTTG ACAAAAGAAC AACCTCAGGC CAAAGCAGGC
    AATGGACAGA ATTCTTGTGG AGTAGAAGAC GTCCTTCAGC TTCTGCGTAT TCTGTATATA
    GTTGCAAGTG ACCCTTATTC AAGAATATCC CAAGAAGAAG GTGATGAGCA GCCTCAGTTT
    ACTTTTCCAC CAGATGAATT CACTAGCAAA AAAATCACAA CAAAAATTTT GCAGCAAATT
    GAGGAACCAT TGGCATTGGC AAGTGGCGCT CTTCCAGACT GGTGTGAACA ACTAACCAGC
    AAGTGTCCTT TTCTAATTCC GTTTGAAACT AGGCAGCTTT ACTTCACATG TACGGCGTTT
    GGTGCATCCA GAGCAATCGT GTGGTTACAA AACCGACGTG AGGCCACTGT AGAAAGAACA
    AGGACCACAA GTAGTGTAAG ACGAGATGAT CCTGGAGAGT TTCGAGTTGG TCGTCTCAAG
    CATGAAAGAG TGAAAGTTCC ACGTGGTGAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020955538.1
    CDS802..7074
    Translation

    Target ORF information:

    RefSeq Version XM_021099879.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021099879.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCAGATG TGGATCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAGATT
    TTTCTTGATG AAAGTGCTCC AGACAACGTG TTAGAGGTGA CCGCCCGTGC CATAACATAC
    TACCTGGACG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTGGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAATGTG TGAAGGTGTT AGAACTGATA TGCACTCGTG AGTCAGGAGC GGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACACCT GGTTCATAAA
    GATACATTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCGTT AGCCAAGCAT GGATTAACTG AGGAATTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT ATCAGGGCCA TCATCTGCTT GCAAACCAGG TCGCAGCACC
    ACGGGAGCGC CTTCCACGGC TGCAGACTCC AAATTGAGTA ACCAGGTGTC AACAATTGTG
    AGCCTGCTCT CAACACTTTG TAGAGGCTCT CCGGTAGTAA CACATGATCT CCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGACG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAGGC TTTGCCAAAG
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTCCGGA GATTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GGGGTCAGAG GTCATCTTCT TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTCTT AAGGCATGGT GCAAACCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAAAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGACACA
    AACAAAGATG AAGAAGAATG TAATGAGCCA AAAGGAGATC CAGAAATGGC ACCCATATAC
    TTGAAAAGGT TACTGCCAGT ATTTGCACAA ACATTTCAGC AGACGATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT TATTCGAAAA ATGATTCATT TTTGTTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCGA CAGTACTAGT GGAGATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGTTGGCTCT GCAGATCATA
    AGAGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACATT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAAGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA GGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT ATGGAGTGAT
    GCGGCAGCCT TGGAATTATC CAATGGAAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GCTACAAAGA GCTCGAGGCC AAGTAAAGCC TTCTACTTCA
    AGTCAGCCTA TACTCTCAGC ACCAGGACCT ACTAAACTCA CTGTAGGGAA TTGGTCACTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCAACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTGTTT GAGTCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA GAGAGGCAGA
    AAACTAAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACCATGGC TCGAGATTTA
    TATGATGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTGATGAAG ACAGCTTTAG AAAATCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATACGAA ATGTGCAGTA GTGGCTTGGT GCAAGCACTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTAGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTC AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTGCTGGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTGCAGA TACTTACAAG AAGATTAAGA
    TTTCGGTTAG AACGTGCACC TGGTGAAACT GCACTAATAG ACAGAACTGG CAGGATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTAGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ATGATTTCGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCGT TTACTGGATT
    GGGACAAATG CCAAAACTGC ATATGAATGG GTAAATCCAG CTGCATATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ATTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCTTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTA
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AAGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGGAAG
    AATGCTAGTG GACAAACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTC
    AACGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAGGC TAATCTTAGA
    AGACAGAGAC GTCTGGTACG CTCCCAGGTA TTGAAGTACA TGGTTCCAGG GGCTCGTGTT
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACC
    GTCACAGGAG AGCTGCACAA TGGCACAACG CAATCATGGA GCAGCTTGGT GAAAAACAAC
    TGTCCAGACA AGACATCTGC TGCTGCAGGC TCCTCAAGTA GAAAAGGAAG CAGCAGTTCT
    GTGTGTAGCG TGGCCAGTAG CAGCGACATC AGCTTGGGTT CGACCAAAAC GGAACGGAGA
    TCAGAAATTG TAATGGAACA CAGTATAGTT TCAGGAGCTG ATGTCCATGA ACCAATTGTT
    GTTCTTTCAT CTGCTGAAAA CGTCCCTCAA ACAGAAGTAG GGTCATCTTC CAGTGCAAGT
    ACCAGCACCT TAACAGCGGA AACGGGAAGT GAAAATGCTG AAAGAAAGTT AGGCACTGAT
    AGTTCTGTTC GTACTCCTGG GGAGTCTAGT GCAATATCCA TGGGGATTGT TAGTGTTAGT
    TCTCCTGATG TTAGTTCAGT ATCTGAGTTG ACTAATAAAG AAGCAACTTC ACAACGGCCC
    CTTAGCTCTT CAGCGAGTAA CAGACTCTCA GTGAGTTCTT TGTTGGCTGC TGGGGCCCCT
    ATGAGCTCTA GTGCAAGTGT ACCTAACCTG TCCTCAAGAG AAACATCTAG CTTGGAGAGT
    TTTGTAAGGA GAGTGGCAAA CATAGCACGG ACTAATGCCA CGAACAACAT GAATCTAAGC
    CGAAGCAGCA GTGATAACAA CACTAATACT TTGGGGAGGA ATGTGATGAG CACAGCAACT
    TCTCCTCTTA TGGGCGCTCA GAGTTTCCCT AATTTGACCA CACCTGGTAC TACATCAACA
    GTGACTATGT CAACGTCCAG TGTTACCAGT AGTAGCAATG TAGCTACAGC AACAACAGTT
    TTATCAGTTG GTCAGTCATT AAGTAATACT TTAACCACCA GCCTCACATC AACCTCCAGT
    GAGAGTGACA CAGGTCAGGA AGCAGAATAT TCCTTATATG ATTTCCTTGA TAGCTGCCGT
    GCCAGTACTC TGTTAGCTGA GCTAGATGAC GATGAGGACT TGCCAGAACC AGATGAGGAA
    GATGATGAAA ATGAAGATGA CAATCAGGAG GACCAAGAAT ACGAGGAGAT TCTGAGACGT
    CCATCTCTGC AGCGTCGAGC TGGCTCCCGC TCTGATGTGA CACACCATGC TGTCACCTCA
    CAGCTACCAC AGGTCCCTGC TGGAGCAGGG AGCCGACCTA TTGGGGAGCA GGAAGAAGAA
    GAGTATGAAA CTAAAGGGGG ACGCCGGAGA ACATGGGATG ATGATTATGT GCTAAAGCGG
    CAGTTTTCAG CTTTGGTTCC TGCTTTTGAT CCTAGACCTG GTCGTACTAA TGTACAGCAG
    ACAACTGATT TAGAAATTCC GCCCCCAGGA ACACCTCATT CAGAGCTCTT GGAAGAAGTT
    GAATGTACTC CATCACCTCG ATTAGCCCTC ACTTTGAAAG TAACAGGTCT TGGAACAACT
    CGTGAGGTTG AATTACCACT CACTAATTTC AGATCAACCA TCTTTTACTA TGTACAGAAA
    CTGCTTCAGT TGTCCTGTAA TGGCAATGTG AAATCAGATA AACTTAGGCG TATTTGGGAG
    CCAACATACA CAATTATGTA CAGAGAAATG AAGGATTCTG ACAAAGAAAA AGAAAATGGA
    AAAATGGGTT GTTGGTCTAT AGAGCATGTG GAACAGTACC TTGGCACTGA TGAATTACCA
    AAGAATGACT TGATAACCTA CCTGCAGAAG AATGCAGACG CCGCTTTCCT GCGCCACTGG
    AAATTAACTG GCACTAATAA AAGTATTAGG AAAAACAGAA ATTGTTCTCA GCTCATAGCT
    GCATATAAGG ACTTTTGTGA ACATGGAACT AAGTCTGGGT TAAACCAGGG GGCCATTTCT
    ACTCTTCAAA GTAGTGATAT TCTTAACTTG ACAAAAGAAC AACCTCAGGC CAAAGCAGGC
    AATGGACAGA ATTCTTGTGG AGTAGAAGAC GTCCTTCAGC TTCTGCGTAT TCTGTATATA
    GTTGCAAGTG ACCCTTATTC AAGAATATCC CAAGAAGAAG GTGATGAGCA GCCTCAGTTT
    ACTTTTCCAC CAGATGAATT CACTAGCAAA AAAATCACAA CAAAAATTTT GCAGCAAATT
    GAGGAACCAT TGGCATTGGC AAGTGGCGCT CTTCCAGACT GGTGTGAACA ACTAACCAGC
    AAGTGTCCTT TTCTAATTCC GTTTGAAACT AGGCAGCTTT ACTTCACATG TACGGCGTTT
    GGTGCATCCA GAGCAATCGT GTGGTTACAA AACCGACGTG AGGCCACTGT AGAAAGAACA
    AGGACCACAA GTAGTGTAAG ACGAGATGAT CCTGGAGAGT TTCGAGTTGG TCGTCTCAAG
    CATGAAAGAG TGAAAGTTCC ACGTGGTGAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe223521
    Clone ID Related Accession (Same CDS sequence) XM_021099880.1
    Accession Version XM_021099880.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6237bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010449.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGCAGATG TGGATCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAGATT
    TTTCTTGATG AAAGTGCTCC AGACAACGTG TTAGAGGTGA CCGCCCGTGC CATAACATAC
    TACCTGGACG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTGGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAATGTG TGAAGGTGTT AGAACTGATA TGCACTCGTG AGTCAGGAGC GGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACACCT GGTTCATAAA
    GATACATTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCGTT AGCCAAGCAT GGATTAACTG AGGAATTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT ATCAGGGCCA TCATCTGCTT GCAAACCAGG TCGCAGCACC
    ACGGGAGCGC CTTCCACGGC TGCAGACTCC AAATTGAGTA ACCAGGTGTC AACAATTGTG
    AGCCTGCTCT CAACACTTTG TAGAGGCTCT CCGGTAGTAA CACATGATCT CCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGACG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAGGC TTTGCCAAAG
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTCCGGA GATTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GGGGTCAGAG GTCATCTTCT TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTCTT AAGGCATGGT GCAAACCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAAAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGACACA
    AACAAAGATG AAGAAGAATG TAATGAGCCA AAAGGAGATC CAGAAATGGC ACCCATATAC
    TTGAAAAGGT TACTGCCAGT ATTTGCACAA ACATTTCAGC AGACGATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT TATTCGAAAA ATGATTCATT TTTGTTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCGA CAGTACTAGT GGAGATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGTTGGCTCT GCAGATCATA
    AGAGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACATT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAAGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA GGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT ATGGAGTGAT
    GCGGCAGCCT TGGAATTATC CAATGGAAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GCTACAAAGA GCTCGAGGCC AAGTAAAGCC TTCTACTTCA
    AGTCAGCCTA TACTCTCAGC ACCAGGACCT ACTAAACTCA CTGTAGGGAA TTGGTCACTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCAACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTGTTT GAGTCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA GAGAGGCAGA
    AAACTAAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACCATGGC TCGAGATTTA
    TATGATGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTGATGAAG ACAGCTTTAG AAAATCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATACGAA ATGTGCAGTA GTGGCTTGGT GCAAGCACTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTAGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTC AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTGCTGGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTGCAGA TACTTACAAG AAGATTAAGA
    TTTCGGTTAG AACGTGCACC TGGTGAAACT GCACTAATAG ACAGAACTGG CAGGATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTAGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ATGATTTCGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCGT TTACTGGATT
    GGGACAAATG CCAAAACTGC ATATGAATGG GTAAATCCAG CTGCATATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ATTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCTTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTA
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AAGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGGAAG
    AATGCTAGTG GACAAACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTC
    AACGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAGGC TAATCTTAGA
    AGACAGAGAC GTCTGGTACG CTCCCAGGTA TTGAAGTACA TGGTTCCAGG GGCTCGTGTT
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACC
    GTCACAGGAG AGCTGCACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCACTGA TAGTTCTGTT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGGATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT GACTAATAAA
    GAAGCAACTT CACAACGGCC CCTTAGCTCT TCAGCGAGTA ACAGACTCTC AGTGAGTTCT
    TTGTTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGCGCTC AGAGTTTCCC TAATTTGACC
    ACACCTGGTA CTACATCAAC AGTGACTATG TCAACGTCCA GTGTTACCAG TAGTAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTT GGTCAGTCAT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAAC CAGATGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGTCCA TCTCTGCAGC GTCGAGCTGG CTCCCGCTCT
    GATGTGACAC ACCATGCTGT CACCTCACAG CTACCACAGG TCCCTGCTGG AGCAGGGAGC
    CGACCTATTG GGGAGCAGGA AGAAGAAGAG TATGAAACTA AAGGGGGACG CCGGAGAACA
    TGGGATGATG ATTATGTGCT AAAGCGGCAG TTTTCAGCTT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GTACTAATGT ACAGCAGACA ACTGATTTAG AAATTCCGCC CCCAGGAACA
    CCTCATTCAG AGCTCTTGGA AGAAGTTGAA TGTACTCCAT CACCTCGATT AGCCCTCACT
    TTGAAAGTAA CAGGTCTTGG AACAACTCGT GAGGTTGAAT TACCACTCAC TAATTTCAGA
    TCAACCATCT TTTACTATGT ACAGAAACTG CTTCAGTTGT CCTGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCA ACATACACAA TTATGTACAG AGAAATGAAG
    GATTCTGACA AAGAAAAAGA AAATGGAAAA ATGGACTTTT GTGAACATGG AACTAAGTCT
    GGGTTAAACC AGGGGGCCAT TTCTACTCTT CAAAGTAGTG ATATTCTTAA CTTGACAAAA
    GAACAACCTC AGGCCAAAGC AGGCAATGGA CAGAATTCTT GTGGAGTAGA AGACGTCCTT
    CAGCTTCTGC GTATTCTGTA TATAGTTGCA AGTGACCCTT ATTCAAGAAT ATCCCAAGAA
    GAAGGTGATG AGCAGCCTCA GTTTACTTTT CCACCAGATG AATTCACTAG CAAAAAAATC
    ACAACAAAAA TTTTGCAGCA AATTGAGGAA CCATTGGCAT TGGCAAGTGG CGCTCTTCCA
    GACTGGTGTG AACAACTAAC CAGCAAGTGT CCTTTTCTAA TTCCGTTTGA AACTAGGCAG
    CTTTACTTCA CATGTACGGC GTTTGGTGCA TCCAGAGCAA TCGTGTGGTT ACAAAACCGA
    CGTGAGGCCA CTGTAGAAAG AACAAGGACC ACAAGTAGTG TAAGACGAGA TGATCCTGGA
    GAGTTTCGAG TTGGTCGTCT CAAGCATGAA AGAGTGAAAG TTCCACGTGG TGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020955539.1
    CDS802..7038
    Translation

    Target ORF information:

    RefSeq Version XM_021099880.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021099880.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGCAGATG TGGATCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAGATT
    TTTCTTGATG AAAGTGCTCC AGACAACGTG TTAGAGGTGA CCGCCCGTGC CATAACATAC
    TACCTGGACG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTGGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAATGTG TGAAGGTGTT AGAACTGATA TGCACTCGTG AGTCAGGAGC GGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACACCT GGTTCATAAA
    GATACATTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCGTT AGCCAAGCAT GGATTAACTG AGGAATTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT ATCAGGGCCA TCATCTGCTT GCAAACCAGG TCGCAGCACC
    ACGGGAGCGC CTTCCACGGC TGCAGACTCC AAATTGAGTA ACCAGGTGTC AACAATTGTG
    AGCCTGCTCT CAACACTTTG TAGAGGCTCT CCGGTAGTAA CACATGATCT CCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGACG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAGGC TTTGCCAAAG
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTCCGGA GATTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GGGGTCAGAG GTCATCTTCT TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTCTT AAGGCATGGT GCAAACCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAAAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGACACA
    AACAAAGATG AAGAAGAATG TAATGAGCCA AAAGGAGATC CAGAAATGGC ACCCATATAC
    TTGAAAAGGT TACTGCCAGT ATTTGCACAA ACATTTCAGC AGACGATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT TATTCGAAAA ATGATTCATT TTTGTTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCGA CAGTACTAGT GGAGATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGTTGGCTCT GCAGATCATA
    AGAGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACATT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAAGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA GGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT ATGGAGTGAT
    GCGGCAGCCT TGGAATTATC CAATGGAAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GCTACAAAGA GCTCGAGGCC AAGTAAAGCC TTCTACTTCA
    AGTCAGCCTA TACTCTCAGC ACCAGGACCT ACTAAACTCA CTGTAGGGAA TTGGTCACTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCAACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTGTTT GAGTCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA GAGAGGCAGA
    AAACTAAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACCATGGC TCGAGATTTA
    TATGATGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTGATGAAG ACAGCTTTAG AAAATCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATACGAA ATGTGCAGTA GTGGCTTGGT GCAAGCACTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTAGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTC AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTGCTGGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTGCAGA TACTTACAAG AAGATTAAGA
    TTTCGGTTAG AACGTGCACC TGGTGAAACT GCACTAATAG ACAGAACTGG CAGGATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTAGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ATGATTTCGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCGT TTACTGGATT
    GGGACAAATG CCAAAACTGC ATATGAATGG GTAAATCCAG CTGCATATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ATTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCTTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTA
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AAGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGGAAG
    AATGCTAGTG GACAAACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTC
    AACGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAGGC TAATCTTAGA
    AGACAGAGAC GTCTGGTACG CTCCCAGGTA TTGAAGTACA TGGTTCCAGG GGCTCGTGTT
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACC
    GTCACAGGAG AGCTGCACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCACTGA TAGTTCTGTT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGGATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT GACTAATAAA
    GAAGCAACTT CACAACGGCC CCTTAGCTCT TCAGCGAGTA ACAGACTCTC AGTGAGTTCT
    TTGTTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGCGCTC AGAGTTTCCC TAATTTGACC
    ACACCTGGTA CTACATCAAC AGTGACTATG TCAACGTCCA GTGTTACCAG TAGTAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTT GGTCAGTCAT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAAC CAGATGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGTCCA TCTCTGCAGC GTCGAGCTGG CTCCCGCTCT
    GATGTGACAC ACCATGCTGT CACCTCACAG CTACCACAGG TCCCTGCTGG AGCAGGGAGC
    CGACCTATTG GGGAGCAGGA AGAAGAAGAG TATGAAACTA AAGGGGGACG CCGGAGAACA
    TGGGATGATG ATTATGTGCT AAAGCGGCAG TTTTCAGCTT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GTACTAATGT ACAGCAGACA ACTGATTTAG AAATTCCGCC CCCAGGAACA
    CCTCATTCAG AGCTCTTGGA AGAAGTTGAA TGTACTCCAT CACCTCGATT AGCCCTCACT
    TTGAAAGTAA CAGGTCTTGG AACAACTCGT GAGGTTGAAT TACCACTCAC TAATTTCAGA
    TCAACCATCT TTTACTATGT ACAGAAACTG CTTCAGTTGT CCTGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCA ACATACACAA TTATGTACAG AGAAATGAAG
    GATTCTGACA AAGAAAAAGA AAATGGAAAA ATGGACTTTT GTGAACATGG AACTAAGTCT
    GGGTTAAACC AGGGGGCCAT TTCTACTCTT CAAAGTAGTG ATATTCTTAA CTTGACAAAA
    GAACAACCTC AGGCCAAAGC AGGCAATGGA CAGAATTCTT GTGGAGTAGA AGACGTCCTT
    CAGCTTCTGC GTATTCTGTA TATAGTTGCA AGTGACCCTT ATTCAAGAAT ATCCCAAGAA
    GAAGGTGATG AGCAGCCTCA GTTTACTTTT CCACCAGATG AATTCACTAG CAAAAAAATC
    ACAACAAAAA TTTTGCAGCA AATTGAGGAA CCATTGGCAT TGGCAAGTGG CGCTCTTCCA
    GACTGGTGTG AACAACTAAC CAGCAAGTGT CCTTTTCTAA TTCCGTTTGA AACTAGGCAG
    CTTTACTTCA CATGTACGGC GTTTGGTGCA TCCAGAGCAA TCGTGTGGTT ACAAAACCGA
    CGTGAGGCCA CTGTAGAAAG AACAAGGACC ACAAGTAGTG TAAGACGAGA TGATCCTGGA
    GAGTTTCGAG TTGGTCGTCT CAAGCATGAA AGAGTGAAAG TTCCACGTGG TGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe223522
    Clone ID Related Accession (Same CDS sequence) XM_021099881.1
    Accession Version XM_021099881.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6231bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010449.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGCAGATG TGGATCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAGATT
    TTTCTTGATG AAAGTGCTCC AGACAACGTG TTAGAGGTGA CCGCCCGTGC CATAACATAC
    TACCTGGACG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTGGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAATGTG TGAAGGTGTT AGAACTGATA TGCACTCGTG AGTCAGGAGC GGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACACCT GGTTCATAAA
    GATACATTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCGTT AGCCAAGCAT GGATTAACTG AGGAATTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT ATCAGGGCCA TCATCTGCTT GCAAACCAGG TCGCAGCACC
    ACGGGAGCGC CTTCCACGGC TGCAGACTCC AAATTGAGTA ACCAGGTGTC AACAATTGTG
    AGCCTGCTCT CAACACTTTG TAGAGGCTCT CCGGTAGTAA CACATGATCT CCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGACG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAGGC TTTGCCAAAG
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTCCGGA GATTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GGGGTCAGAG GTCATCTTCT TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTCTT AAGGCATGGT GCAAACCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAAAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGACACA
    AACAAAGATG AAGAAGAATG TAATGAGCCA AAAGGAGATC CAGAAATGGC ACCCATATAC
    TTGAAAAGGT TACTGCCAGT ATTTGCACAA ACATTTCAGC AGACGATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT TATTCGAAAA ATGATTCATT TTTGTTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCGA CAGTACTAGT GGAGATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGTTGGCTCT GCAGATCATA
    AGAGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACATT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAAGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA GGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT ATGGAGTGAT
    GCGGCAGCCT TGGAATTATC CAATGGAAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GCTACAAAGA GCTCGAGGCC AAGTAAAGCC TTCTACTTCA
    AGTCAGCCTA TACTCTCAGC ACCAGGACCT ACTAAACTCA CTGTAGGGAA TTGGTCACTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCAACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTGTTT GAGTCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA GAGAGGCAGA
    AAACTAAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACCATGGC TCGAGATTTA
    TATGATGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTGATGAAG ACAGCTTTAG AAAATCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATACGAA ATGTGCAGTA GTGGCTTGGT GCAAGCACTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTAGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTC AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTGCTGGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTGCAGA TACTTACAAG AAGATTAAGA
    TTTCGGTTAG AACGTGCACC TGGTGAAACT GCACTAATAG ACAGAACTGG CAGGATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTAGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ATGATTTCGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCGT TTACTGGATT
    GGGACAAATG CCAAAACTGC ATATGAATGG GTAAATCCAG CTGCATATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ATTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCTTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTA
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AAGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGGAAG
    AATGCTAGTG GACAAACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTC
    AACGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAGGC TAATCTTAGA
    AGACAGAGAC GTCTGGTACG CTCCCAGGTA TTGAAGTACA TGGTTCCAGG GGCTCGTGTT
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACC
    GTCACAGGAG AGCTGCACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCACTGA TAGTTCTGTT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGGATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT GACTAATAAA
    GAAGCAACTT CACAACGGCC CCTTAGCTCT TCAGCGAGTA ACAGACTCTC AGTGAGTTCT
    TTGTTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGCGCTC AGAGTTTCCC TAATTTGACC
    ACACCTGGTA CTACATCAAC AGTGACTATG TCAACGTCCA GTGTTACCAG TAGTAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTT GGTCAGTCAT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAAC CAGATGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGA TTCTGAGACG TCCATCTCTG CAGCGTCGAG CTGGCTCCCG CTCTGATGTG
    ACACACCATG CTGTCACCTC ACAGCTACCA CAGGTCCCTG CTGGAGCAGG GAGCCGACCT
    ATTGGGGAGC AGGAAGAAGA AGAGTATGAA ACTAAAGGGG GACGCCGGAG AACATGGGAT
    GATGATTATG TGCTAAAGCG GCAGTTTTCA GCTTTGGTTC CTGCTTTTGA TCCTAGACCT
    GGTCGTACTA ATGTACAGCA GACAACTGAT TTAGAAATTC CGCCCCCAGG AACACCTCAT
    TCAGAGCTCT TGGAAGAAGT TGAATGTACT CCATCACCTC GATTAGCCCT CACTTTGAAA
    GTAACAGGTC TTGGAACAAC TCGTGAGGTT GAATTACCAC TCACTAATTT CAGATCAACC
    ATCTTTTACT ATGTACAGAA ACTGCTTCAG TTGTCCTGTA ATGGCAATGT GAAATCAGAT
    AAACTTAGGC GTATTTGGGA GCCAACATAC ACAATTATGT ACAGAGAAAT GAAGGATTCT
    GACAAAGAAA AAGAAAATGG AAAAATGGAC TTTTGTGAAC ATGGAACTAA GTCTGGGTTA
    AACCAGGGGG CCATTTCTAC TCTTCAAAGT AGTGATATTC TTAACTTGAC AAAAGAACAA
    CCTCAGGCCA AAGCAGGCAA TGGACAGAAT TCTTGTGGAG TAGAAGACGT CCTTCAGCTT
    CTGCGTATTC TGTATATAGT TGCAAGTGAC CCTTATTCAA GAATATCCCA AGAAGAAGGT
    GATGAGCAGC CTCAGTTTAC TTTTCCACCA GATGAATTCA CTAGCAAAAA AATCACAACA
    AAAATTTTGC AGCAAATTGA GGAACCATTG GCATTGGCAA GTGGCGCTCT TCCAGACTGG
    TGTGAACAAC TAACCAGCAA GTGTCCTTTT CTAATTCCGT TTGAAACTAG GCAGCTTTAC
    TTCACATGTA CGGCGTTTGG TGCATCCAGA GCAATCGTGT GGTTACAAAA CCGACGTGAG
    GCCACTGTAG AAAGAACAAG GACCACAAGT AGTGTAAGAC GAGATGATCC TGGAGAGTTT
    CGAGTTGGTC GTCTCAAGCA TGAAAGAGTG AAAGTTCCAC GTGGTGAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020955540.1
    CDS802..7032
    Translation

    Target ORF information:

    RefSeq Version XM_021099881.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021099881.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGCAGATG TGGATCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAGATT
    TTTCTTGATG AAAGTGCTCC AGACAACGTG TTAGAGGTGA CCGCCCGTGC CATAACATAC
    TACCTGGACG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTGGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAATGTG TGAAGGTGTT AGAACTGATA TGCACTCGTG AGTCAGGAGC GGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACACCT GGTTCATAAA
    GATACATTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCGTT AGCCAAGCAT GGATTAACTG AGGAATTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT ATCAGGGCCA TCATCTGCTT GCAAACCAGG TCGCAGCACC
    ACGGGAGCGC CTTCCACGGC TGCAGACTCC AAATTGAGTA ACCAGGTGTC AACAATTGTG
    AGCCTGCTCT CAACACTTTG TAGAGGCTCT CCGGTAGTAA CACATGATCT CCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGACG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAGGC TTTGCCAAAG
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTCCGGA GATTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GGGGTCAGAG GTCATCTTCT TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTCTT AAGGCATGGT GCAAACCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAAAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGACACA
    AACAAAGATG AAGAAGAATG TAATGAGCCA AAAGGAGATC CAGAAATGGC ACCCATATAC
    TTGAAAAGGT TACTGCCAGT ATTTGCACAA ACATTTCAGC AGACGATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT TATTCGAAAA ATGATTCATT TTTGTTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCGA CAGTACTAGT GGAGATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGTTGGCTCT GCAGATCATA
    AGAGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACATT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAAGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA GGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT ATGGAGTGAT
    GCGGCAGCCT TGGAATTATC CAATGGAAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GCTACAAAGA GCTCGAGGCC AAGTAAAGCC TTCTACTTCA
    AGTCAGCCTA TACTCTCAGC ACCAGGACCT ACTAAACTCA CTGTAGGGAA TTGGTCACTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCAACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTGTTT GAGTCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA GAGAGGCAGA
    AAACTAAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACCATGGC TCGAGATTTA
    TATGATGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTGATGAAG ACAGCTTTAG AAAATCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATACGAA ATGTGCAGTA GTGGCTTGGT GCAAGCACTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTAGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTC AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTGCTGGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTGCAGA TACTTACAAG AAGATTAAGA
    TTTCGGTTAG AACGTGCACC TGGTGAAACT GCACTAATAG ACAGAACTGG CAGGATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTAGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ATGATTTCGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCGT TTACTGGATT
    GGGACAAATG CCAAAACTGC ATATGAATGG GTAAATCCAG CTGCATATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ATTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCTTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTA
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AAGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGGAAG
    AATGCTAGTG GACAAACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTC
    AACGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAGGC TAATCTTAGA
    AGACAGAGAC GTCTGGTACG CTCCCAGGTA TTGAAGTACA TGGTTCCAGG GGCTCGTGTT
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACC
    GTCACAGGAG AGCTGCACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCACTGA TAGTTCTGTT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGGATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT GACTAATAAA
    GAAGCAACTT CACAACGGCC CCTTAGCTCT TCAGCGAGTA ACAGACTCTC AGTGAGTTCT
    TTGTTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGCGCTC AGAGTTTCCC TAATTTGACC
    ACACCTGGTA CTACATCAAC AGTGACTATG TCAACGTCCA GTGTTACCAG TAGTAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTT GGTCAGTCAT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAAC CAGATGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGA TTCTGAGACG TCCATCTCTG CAGCGTCGAG CTGGCTCCCG CTCTGATGTG
    ACACACCATG CTGTCACCTC ACAGCTACCA CAGGTCCCTG CTGGAGCAGG GAGCCGACCT
    ATTGGGGAGC AGGAAGAAGA AGAGTATGAA ACTAAAGGGG GACGCCGGAG AACATGGGAT
    GATGATTATG TGCTAAAGCG GCAGTTTTCA GCTTTGGTTC CTGCTTTTGA TCCTAGACCT
    GGTCGTACTA ATGTACAGCA GACAACTGAT TTAGAAATTC CGCCCCCAGG AACACCTCAT
    TCAGAGCTCT TGGAAGAAGT TGAATGTACT CCATCACCTC GATTAGCCCT CACTTTGAAA
    GTAACAGGTC TTGGAACAAC TCGTGAGGTT GAATTACCAC TCACTAATTT CAGATCAACC
    ATCTTTTACT ATGTACAGAA ACTGCTTCAG TTGTCCTGTA ATGGCAATGT GAAATCAGAT
    AAACTTAGGC GTATTTGGGA GCCAACATAC ACAATTATGT ACAGAGAAAT GAAGGATTCT
    GACAAAGAAA AAGAAAATGG AAAAATGGAC TTTTGTGAAC ATGGAACTAA GTCTGGGTTA
    AACCAGGGGG CCATTTCTAC TCTTCAAAGT AGTGATATTC TTAACTTGAC AAAAGAACAA
    CCTCAGGCCA AAGCAGGCAA TGGACAGAAT TCTTGTGGAG TAGAAGACGT CCTTCAGCTT
    CTGCGTATTC TGTATATAGT TGCAAGTGAC CCTTATTCAA GAATATCCCA AGAAGAAGGT
    GATGAGCAGC CTCAGTTTAC TTTTCCACCA GATGAATTCA CTAGCAAAAA AATCACAACA
    AAAATTTTGC AGCAAATTGA GGAACCATTG GCATTGGCAA GTGGCGCTCT TCCAGACTGG
    TGTGAACAAC TAACCAGCAA GTGTCCTTTT CTAATTCCGT TTGAAACTAG GCAGCTTTAC
    TTCACATGTA CGGCGTTTGG TGCATCCAGA GCAATCGTGT GGTTACAAAA CCGACGTGAG
    GCCACTGTAG AAAGAACAAG GACCACAAGT AGTGTAAGAC GAGATGATCC TGGAGAGTTT
    CGAGTTGGTC GTCTCAAGCA TGAAAGAGTG AAAGTTCCAC GTGGTGAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe223523
    Clone ID Related Accession (Same CDS sequence) XM_021099882.1
    Accession Version XM_021099882.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6096bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010449.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCAGATG TGGATCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAGATT
    TTTCTTGATG AAAGTGCTCC AGACAACGTG TTAGAGGTGA CCGCCCGTGC CATAACATAC
    TACCTGGACG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTGGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAATGTG TGAAGGTGTT AGAACTGATA TGCACTCGTG AGTCAGGAGC GGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACACCT GGTTCATAAA
    GATACATTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCGTT AGCCAAGCAT GGATTAACTG AGGAATTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT ATCAGGGCCA TCATCTGCTT GCAAACCAGG TCGCAGCACC
    ACGGGAGCGC CTTCCACGGC TGCAGACTCC AAATTGAGTA ACCAGGTGTC AACAATTGTG
    AGCCTGCTCT CAACACTTTG TAGAGGCTCT CCGGTAGTAA CACATGATCT CCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGACG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAGGC TTTGCCAAAG
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTCCGGA GATTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GGGGTCAGAG GTCATCTTCT TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTCTT AAGGCATGGT GCAAACCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAAAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGACACA
    AACAAAGATG AAGAAGAATG TAATGAGCCA AAAGGAGATC CAGAAATGGC ACCCATATAC
    TTGAAAAGGT TACTGCCAGT ATTTGCACAA ACATTTCAGC AGACGATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT TATTCGAAAA ATGATTCATT TTTGTTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCGA CAGTACTAGT GGAGATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGTTGGCTCT GCAGATCATA
    AGAGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACATT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAAGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA GGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT ATGGAGTGAT
    GCGGCAGCCT TGGAATTATC CAATGGAAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GCTACAAAGA GCTCGAGGCC AAGTAAAGCC TTCTACTTCA
    AGTCAGCCTA TACTCTCAGC ACCAGGACCT ACTAAACTCA CTGTAGGGAA TTGGTCACTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCAACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTGTTT GAGTCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA GAGAGGCAGA
    AAACTAAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACCATGGC TCGAGATTTA
    TATGATGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTGATGAAG ACAGCTTTAG AAAATCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATACGAA ATGTGCAGTA GTGGCTTGGT GCAAGCACTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTAGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTC AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTGCTGGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTGCAGA TACTTACAAG AAGATTAAGA
    TTTCGGTTAG AACGTGCACC TGGTGAAACT GCACTAATAG ACAGAACTGG CAGGATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTAGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ATGATTTCGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCGT TTACTGGATT
    GGGACAAATG CCAAAACTGC ATATGAATGG GTAAATCCAG CTGCATATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ATTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCTTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTA
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AAGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGGAAG
    AATGCTAGTG GACAAACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTC
    AACGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAGGC TAATCTTAGA
    AGACAGAGAC GTCTGGTACG CTCCCAGGTA TTGAAGTACA TGGTTCCAGG GGCTCGTGTT
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACC
    GTCACAGGAG AGCTGCACAA TGGCACAACG CAATCATGGA GCAGCTTGGT GAAAAACAAC
    TGTCCAGACA AGACATCTGC TGCTGCAGGC TCCTCAAGTA GAAAAGGAAG CAGCAGTTCT
    GTGTGTAGCG TGGCCAGTAG CAGCGACATC AGCTTGGGTT CGACCAAAAC GGAACGGAGA
    TCAGAAATTG TAATGGAACA CAGTATAGTT TCAGGAGCTG ATGTCCATGA ACCAATTGTT
    GTTCTTTCAT CTGCTGAAAA CGTCCCTCAA ACAGAAGTAG GGTCATCTTC CAGTGCAAGT
    ACCAGCACCT TAACAGCGGA AACGGGAAGT GAAAATGCTG AAAGAAAGTT AGGCACTGAT
    AGTTCTGTTC GTACTCCTGG GGAGTCTAGT GCAATATCCA TGGGGATTGT TAGTGTTAGT
    TCTCCTGATG TTAGTTCAGT ATCTGAGTTG ACTAATAAAG AAGCAACTTC ACAACGGCCC
    CTTAGCTCTT CAGCGAGTAA CAGACTCTCA GTGAGTTCTT TGTTGGCTGC TGGGGCCCCT
    ATGAGCTCTA GTGCAAGTGT ACCTAACCTG TCCTCAAGAG AAACATCTAG CTTGGAGAGT
    TTTGTAAGGA GAGTGGCAAA CATAGCACGG ACTAATGCCA CGAACAACAT GAATCTAAGC
    CGAAGCAGCA GTGATAACAA CACTAATACT TTGGGGAGGA ATGTGATGAG CACAGCAACT
    TCTCCTCTTA TGGGCGCTCA GAGTTTCCCT AATTTGACCA CACCTGGTAC TACATCAACA
    GTGACTATGT CAACGTCCAG TGTTACCAGT AGTAGCAATG TAGCTACAGC AACAACAGTT
    TTATCAGTTG GTCAGTCATT AAGTAATACT TTAACCACCA GCCTCACATC AACCTCCAGT
    GAGAGTGACA CAGGTCAGGA AGCAGAATAT TCCTTATATG ATTTCCTTGA TAGCTGCCGT
    GCCAGTACTC TGTTAGCTGA GCTAGATGAC GATGAGGACT TGCCAGAACC AGATGAGGAA
    GATGATGAAA ATGAAGATGA CAATCAGGAG GACCAAGAAT ACGAGGAGGT TATGATTCTG
    AGACGTCCAT CTCTGCAGCG TCGAGCTGGC TCCCGCTCTG ATGTGACACA CCATGCTGTC
    ACCTCACAGC TACCACAGGT CCCTGCTGGA GCAGGGAGCC GACCTATTGG GGAGCAGGAA
    GAAGAAGAGT ATGAAACTAA AGGGGGACGC CGGAGAACAT GGGATGATGA TTATGTGCTA
    AAGCGGCAGT TTTCAGCTTT GGTTCCTGCT TTTGATCCTA GACCTGGTCG TACTAATGTA
    CAGCAGACAA CTGATTTAGA AATTCCGCCC CCAGGAACAC CTCATTCAGA GCTCTTGGAA
    GAAGTTGAAT GTACTCCATC ACCTCGATTA GCCCTCACTT TGAAAGTAAC AGGTCTTGGA
    ACAACTCGTG AGGTTGAATT ACCACTCACT AATTTCAGAT CAACCATCTT TTACTATGTA
    CAGAAACTGC TTCAGTTGTC CTGTAATGGC AATGTGAAAT CAGATAAACT TAGGCGTATT
    TGGGAGCCAA CATACACAAT TATGTACAGA GAAATGAAGG ATTCTGACAA AGAAAAAGAA
    AATGGAAAAA TGGACTTTTG TGAACATGGA ACTAAGTCTG GGTTAAACCA GGGGGCCATT
    TCTACTCTTC AAAGTAGTGA TATTCTTAAC TTGACAAAAG AACAACCTCA GGCCAAAGCA
    GGCAATGGAC AGAATTCTTG TGGAGTAGAA GACGTCCTTC AGCTTCTGCG TATTCTGTAT
    ATAGTTGCAA GTGACCCTTA TTCAAGAATA TCCCAAGAAG AAGGTGATGA GCAGCCTCAG
    TTTACTTTTC CACCAGATGA ATTCACTAGC AAAAAAATCA CAACAAAAAT TTTGCAGCAA
    ATTGAGGAAC CATTGGCATT GGCAAGTGGC GCTCTTCCAG ACTGGTGTGA ACAACTAACC
    AGCAAGTGTC CTTTTCTAAT TCCGTTTGAA ACTAGGCAGC TTTACTTCAC ATGTACGGCG
    TTTGGTGCAT CCAGAGCAAT CGTGTGGTTA CAAAACCGAC GTGAGGCCAC TGTAGAAAGA
    ACAAGGACCA CAAGTAGTGT AAGACGAGAT GATCCTGGAG AGTTTCGAGT TGGTCGTCTC
    AAGCATGAAA GAGTGAAAGT TCCACGTGGT GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020955541.1
    CDS802..6897
    Translation

    Target ORF information:

    RefSeq Version XM_021099882.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021099882.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCAGATG TGGATCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAGATT
    TTTCTTGATG AAAGTGCTCC AGACAACGTG TTAGAGGTGA CCGCCCGTGC CATAACATAC
    TACCTGGACG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTGGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAATGTG TGAAGGTGTT AGAACTGATA TGCACTCGTG AGTCAGGAGC GGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACACCT GGTTCATAAA
    GATACATTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCGTT AGCCAAGCAT GGATTAACTG AGGAATTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT ATCAGGGCCA TCATCTGCTT GCAAACCAGG TCGCAGCACC
    ACGGGAGCGC CTTCCACGGC TGCAGACTCC AAATTGAGTA ACCAGGTGTC AACAATTGTG
    AGCCTGCTCT CAACACTTTG TAGAGGCTCT CCGGTAGTAA CACATGATCT CCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGACG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAGGC TTTGCCAAAG
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTCCGGA GATTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GGGGTCAGAG GTCATCTTCT TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTCTT AAGGCATGGT GCAAACCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAAAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGACACA
    AACAAAGATG AAGAAGAATG TAATGAGCCA AAAGGAGATC CAGAAATGGC ACCCATATAC
    TTGAAAAGGT TACTGCCAGT ATTTGCACAA ACATTTCAGC AGACGATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT TATTCGAAAA ATGATTCATT TTTGTTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCGA CAGTACTAGT GGAGATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGTTGGCTCT GCAGATCATA
    AGAGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACATT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAAGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA GGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT ATGGAGTGAT
    GCGGCAGCCT TGGAATTATC CAATGGAAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GCTACAAAGA GCTCGAGGCC AAGTAAAGCC TTCTACTTCA
    AGTCAGCCTA TACTCTCAGC ACCAGGACCT ACTAAACTCA CTGTAGGGAA TTGGTCACTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCAACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTGTTT GAGTCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA GAGAGGCAGA
    AAACTAAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACCATGGC TCGAGATTTA
    TATGATGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTGATGAAG ACAGCTTTAG AAAATCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATACGAA ATGTGCAGTA GTGGCTTGGT GCAAGCACTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTAGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTC AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTGCTGGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTGCAGA TACTTACAAG AAGATTAAGA
    TTTCGGTTAG AACGTGCACC TGGTGAAACT GCACTAATAG ACAGAACTGG CAGGATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTAGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ATGATTTCGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCGT TTACTGGATT
    GGGACAAATG CCAAAACTGC ATATGAATGG GTAAATCCAG CTGCATATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ATTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCTTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTA
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AAGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGGAAG
    AATGCTAGTG GACAAACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTC
    AACGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAGGC TAATCTTAGA
    AGACAGAGAC GTCTGGTACG CTCCCAGGTA TTGAAGTACA TGGTTCCAGG GGCTCGTGTT
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACC
    GTCACAGGAG AGCTGCACAA TGGCACAACG CAATCATGGA GCAGCTTGGT GAAAAACAAC
    TGTCCAGACA AGACATCTGC TGCTGCAGGC TCCTCAAGTA GAAAAGGAAG CAGCAGTTCT
    GTGTGTAGCG TGGCCAGTAG CAGCGACATC AGCTTGGGTT CGACCAAAAC GGAACGGAGA
    TCAGAAATTG TAATGGAACA CAGTATAGTT TCAGGAGCTG ATGTCCATGA ACCAATTGTT
    GTTCTTTCAT CTGCTGAAAA CGTCCCTCAA ACAGAAGTAG GGTCATCTTC CAGTGCAAGT
    ACCAGCACCT TAACAGCGGA AACGGGAAGT GAAAATGCTG AAAGAAAGTT AGGCACTGAT
    AGTTCTGTTC GTACTCCTGG GGAGTCTAGT GCAATATCCA TGGGGATTGT TAGTGTTAGT
    TCTCCTGATG TTAGTTCAGT ATCTGAGTTG ACTAATAAAG AAGCAACTTC ACAACGGCCC
    CTTAGCTCTT CAGCGAGTAA CAGACTCTCA GTGAGTTCTT TGTTGGCTGC TGGGGCCCCT
    ATGAGCTCTA GTGCAAGTGT ACCTAACCTG TCCTCAAGAG AAACATCTAG CTTGGAGAGT
    TTTGTAAGGA GAGTGGCAAA CATAGCACGG ACTAATGCCA CGAACAACAT GAATCTAAGC
    CGAAGCAGCA GTGATAACAA CACTAATACT TTGGGGAGGA ATGTGATGAG CACAGCAACT
    TCTCCTCTTA TGGGCGCTCA GAGTTTCCCT AATTTGACCA CACCTGGTAC TACATCAACA
    GTGACTATGT CAACGTCCAG TGTTACCAGT AGTAGCAATG TAGCTACAGC AACAACAGTT
    TTATCAGTTG GTCAGTCATT AAGTAATACT TTAACCACCA GCCTCACATC AACCTCCAGT
    GAGAGTGACA CAGGTCAGGA AGCAGAATAT TCCTTATATG ATTTCCTTGA TAGCTGCCGT
    GCCAGTACTC TGTTAGCTGA GCTAGATGAC GATGAGGACT TGCCAGAACC AGATGAGGAA
    GATGATGAAA ATGAAGATGA CAATCAGGAG GACCAAGAAT ACGAGGAGGT TATGATTCTG
    AGACGTCCAT CTCTGCAGCG TCGAGCTGGC TCCCGCTCTG ATGTGACACA CCATGCTGTC
    ACCTCACAGC TACCACAGGT CCCTGCTGGA GCAGGGAGCC GACCTATTGG GGAGCAGGAA
    GAAGAAGAGT ATGAAACTAA AGGGGGACGC CGGAGAACAT GGGATGATGA TTATGTGCTA
    AAGCGGCAGT TTTCAGCTTT GGTTCCTGCT TTTGATCCTA GACCTGGTCG TACTAATGTA
    CAGCAGACAA CTGATTTAGA AATTCCGCCC CCAGGAACAC CTCATTCAGA GCTCTTGGAA
    GAAGTTGAAT GTACTCCATC ACCTCGATTA GCCCTCACTT TGAAAGTAAC AGGTCTTGGA
    ACAACTCGTG AGGTTGAATT ACCACTCACT AATTTCAGAT CAACCATCTT TTACTATGTA
    CAGAAACTGC TTCAGTTGTC CTGTAATGGC AATGTGAAAT CAGATAAACT TAGGCGTATT
    TGGGAGCCAA CATACACAAT TATGTACAGA GAAATGAAGG ATTCTGACAA AGAAAAAGAA
    AATGGAAAAA TGGACTTTTG TGAACATGGA ACTAAGTCTG GGTTAAACCA GGGGGCCATT
    TCTACTCTTC AAAGTAGTGA TATTCTTAAC TTGACAAAAG AACAACCTCA GGCCAAAGCA
    GGCAATGGAC AGAATTCTTG TGGAGTAGAA GACGTCCTTC AGCTTCTGCG TATTCTGTAT
    ATAGTTGCAA GTGACCCTTA TTCAAGAATA TCCCAAGAAG AAGGTGATGA GCAGCCTCAG
    TTTACTTTTC CACCAGATGA ATTCACTAGC AAAAAAATCA CAACAAAAAT TTTGCAGCAA
    ATTGAGGAAC CATTGGCATT GGCAAGTGGC GCTCTTCCAG ACTGGTGTGA ACAACTAACC
    AGCAAGTGTC CTTTTCTAAT TCCGTTTGAA ACTAGGCAGC TTTACTTCAC ATGTACGGCG
    TTTGGTGCAT CCAGAGCAAT CGTGTGGTTA CAAAACCGAC GTGAGGCCAC TGTAGAAAGA
    ACAAGGACCA CAAGTAGTGT AAGACGAGAT GATCCTGGAG AGTTTCGAGT TGGTCGTCTC
    AAGCATGAAA GAGTGAAAGT TCCACGTGGT GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe223524
    Clone ID Related Accession (Same CDS sequence) XM_021099883.1
    Accession Version XM_021099883.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6090bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010449.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCAGATG TGGATCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAGATT
    TTTCTTGATG AAAGTGCTCC AGACAACGTG TTAGAGGTGA CCGCCCGTGC CATAACATAC
    TACCTGGACG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTGGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAATGTG TGAAGGTGTT AGAACTGATA TGCACTCGTG AGTCAGGAGC GGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACACCT GGTTCATAAA
    GATACATTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCGTT AGCCAAGCAT GGATTAACTG AGGAATTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT ATCAGGGCCA TCATCTGCTT GCAAACCAGG TCGCAGCACC
    ACGGGAGCGC CTTCCACGGC TGCAGACTCC AAATTGAGTA ACCAGGTGTC AACAATTGTG
    AGCCTGCTCT CAACACTTTG TAGAGGCTCT CCGGTAGTAA CACATGATCT CCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGACG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAGGC TTTGCCAAAG
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTCCGGA GATTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GGGGTCAGAG GTCATCTTCT TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTCTT AAGGCATGGT GCAAACCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAAAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGACACA
    AACAAAGATG AAGAAGAATG TAATGAGCCA AAAGGAGATC CAGAAATGGC ACCCATATAC
    TTGAAAAGGT TACTGCCAGT ATTTGCACAA ACATTTCAGC AGACGATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT TATTCGAAAA ATGATTCATT TTTGTTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCGA CAGTACTAGT GGAGATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGTTGGCTCT GCAGATCATA
    AGAGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACATT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAAGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA GGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT ATGGAGTGAT
    GCGGCAGCCT TGGAATTATC CAATGGAAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GCTACAAAGA GCTCGAGGCC AAGTAAAGCC TTCTACTTCA
    AGTCAGCCTA TACTCTCAGC ACCAGGACCT ACTAAACTCA CTGTAGGGAA TTGGTCACTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCAACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTGTTT GAGTCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA GAGAGGCAGA
    AAACTAAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACCATGGC TCGAGATTTA
    TATGATGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTGATGAAG ACAGCTTTAG AAAATCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATACGAA ATGTGCAGTA GTGGCTTGGT GCAAGCACTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTAGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTC AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTGCTGGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTGCAGA TACTTACAAG AAGATTAAGA
    TTTCGGTTAG AACGTGCACC TGGTGAAACT GCACTAATAG ACAGAACTGG CAGGATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTAGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ATGATTTCGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCGT TTACTGGATT
    GGGACAAATG CCAAAACTGC ATATGAATGG GTAAATCCAG CTGCATATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ATTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCTTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTA
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AAGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGGAAG
    AATGCTAGTG GACAAACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTC
    AACGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAGGC TAATCTTAGA
    AGACAGAGAC GTCTGGTACG CTCCCAGGTA TTGAAGTACA TGGTTCCAGG GGCTCGTGTT
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACC
    GTCACAGGAG AGCTGCACAA TGGCACAACG CAATCATGGA GCAGCTTGGT GAAAAACAAC
    TGTCCAGACA AGACATCTGC TGCTGCAGGC TCCTCAAGTA GAAAAGGAAG CAGCAGTTCT
    GTGTGTAGCG TGGCCAGTAG CAGCGACATC AGCTTGGGTT CGACCAAAAC GGAACGGAGA
    TCAGAAATTG TAATGGAACA CAGTATAGTT TCAGGAGCTG ATGTCCATGA ACCAATTGTT
    GTTCTTTCAT CTGCTGAAAA CGTCCCTCAA ACAGAAGTAG GGTCATCTTC CAGTGCAAGT
    ACCAGCACCT TAACAGCGGA AACGGGAAGT GAAAATGCTG AAAGAAAGTT AGGCACTGAT
    AGTTCTGTTC GTACTCCTGG GGAGTCTAGT GCAATATCCA TGGGGATTGT TAGTGTTAGT
    TCTCCTGATG TTAGTTCAGT ATCTGAGTTG ACTAATAAAG AAGCAACTTC ACAACGGCCC
    CTTAGCTCTT CAGCGAGTAA CAGACTCTCA GTGAGTTCTT TGTTGGCTGC TGGGGCCCCT
    ATGAGCTCTA GTGCAAGTGT ACCTAACCTG TCCTCAAGAG AAACATCTAG CTTGGAGAGT
    TTTGTAAGGA GAGTGGCAAA CATAGCACGG ACTAATGCCA CGAACAACAT GAATCTAAGC
    CGAAGCAGCA GTGATAACAA CACTAATACT TTGGGGAGGA ATGTGATGAG CACAGCAACT
    TCTCCTCTTA TGGGCGCTCA GAGTTTCCCT AATTTGACCA CACCTGGTAC TACATCAACA
    GTGACTATGT CAACGTCCAG TGTTACCAGT AGTAGCAATG TAGCTACAGC AACAACAGTT
    TTATCAGTTG GTCAGTCATT AAGTAATACT TTAACCACCA GCCTCACATC AACCTCCAGT
    GAGAGTGACA CAGGTCAGGA AGCAGAATAT TCCTTATATG ATTTCCTTGA TAGCTGCCGT
    GCCAGTACTC TGTTAGCTGA GCTAGATGAC GATGAGGACT TGCCAGAACC AGATGAGGAA
    GATGATGAAA ATGAAGATGA CAATCAGGAG GACCAAGAAT ACGAGGAGAT TCTGAGACGT
    CCATCTCTGC AGCGTCGAGC TGGCTCCCGC TCTGATGTGA CACACCATGC TGTCACCTCA
    CAGCTACCAC AGGTCCCTGC TGGAGCAGGG AGCCGACCTA TTGGGGAGCA GGAAGAAGAA
    GAGTATGAAA CTAAAGGGGG ACGCCGGAGA ACATGGGATG ATGATTATGT GCTAAAGCGG
    CAGTTTTCAG CTTTGGTTCC TGCTTTTGAT CCTAGACCTG GTCGTACTAA TGTACAGCAG
    ACAACTGATT TAGAAATTCC GCCCCCAGGA ACACCTCATT CAGAGCTCTT GGAAGAAGTT
    GAATGTACTC CATCACCTCG ATTAGCCCTC ACTTTGAAAG TAACAGGTCT TGGAACAACT
    CGTGAGGTTG AATTACCACT CACTAATTTC AGATCAACCA TCTTTTACTA TGTACAGAAA
    CTGCTTCAGT TGTCCTGTAA TGGCAATGTG AAATCAGATA AACTTAGGCG TATTTGGGAG
    CCAACATACA CAATTATGTA CAGAGAAATG AAGGATTCTG ACAAAGAAAA AGAAAATGGA
    AAAATGGACT TTTGTGAACA TGGAACTAAG TCTGGGTTAA ACCAGGGGGC CATTTCTACT
    CTTCAAAGTA GTGATATTCT TAACTTGACA AAAGAACAAC CTCAGGCCAA AGCAGGCAAT
    GGACAGAATT CTTGTGGAGT AGAAGACGTC CTTCAGCTTC TGCGTATTCT GTATATAGTT
    GCAAGTGACC CTTATTCAAG AATATCCCAA GAAGAAGGTG ATGAGCAGCC TCAGTTTACT
    TTTCCACCAG ATGAATTCAC TAGCAAAAAA ATCACAACAA AAATTTTGCA GCAAATTGAG
    GAACCATTGG CATTGGCAAG TGGCGCTCTT CCAGACTGGT GTGAACAACT AACCAGCAAG
    TGTCCTTTTC TAATTCCGTT TGAAACTAGG CAGCTTTACT TCACATGTAC GGCGTTTGGT
    GCATCCAGAG CAATCGTGTG GTTACAAAAC CGACGTGAGG CCACTGTAGA AAGAACAAGG
    ACCACAAGTA GTGTAAGACG AGATGATCCT GGAGAGTTTC GAGTTGGTCG TCTCAAGCAT
    GAAAGAGTGA AAGTTCCACG TGGTGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020955542.1
    CDS802..6891
    Translation

    Target ORF information:

    RefSeq Version XM_021099883.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021099883.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCAGATG TGGATCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAGATT
    TTTCTTGATG AAAGTGCTCC AGACAACGTG TTAGAGGTGA CCGCCCGTGC CATAACATAC
    TACCTGGACG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTGGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAATGTG TGAAGGTGTT AGAACTGATA TGCACTCGTG AGTCAGGAGC GGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACACCT GGTTCATAAA
    GATACATTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCATCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCGTT AGCCAAGCAT GGATTAACTG AGGAATTGTT ATCTCGAATG
    GCTGCTGCTG GTGGTACTGT ATCAGGGCCA TCATCTGCTT GCAAACCAGG TCGCAGCACC
    ACGGGAGCGC CTTCCACGGC TGCAGACTCC AAATTGAGTA ACCAGGTGTC AACAATTGTG
    AGCCTGCTCT CAACACTTTG TAGAGGCTCT CCGGTAGTAA CACATGATCT CCTGAGGTCG
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGACG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAGGC TTTGCCAAAG
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTCCGGA GATTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GGGGTCAGAG GTCATCTTCT TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTCTT AAGGCATGGT GCAAACCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGGGAAAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGACACA
    AACAAAGATG AAGAAGAATG TAATGAGCCA AAAGGAGATC CAGAAATGGC ACCCATATAC
    TTGAAAAGGT TACTGCCAGT ATTTGCACAA ACATTTCAGC AGACGATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT TATTCGAAAA ATGATTCATT TTTGTTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCGA CAGTACTAGT GGAGATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGTTGGCTCT GCAGATCATA
    AGAGATTTAG TAGATAAAGG TGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAACATT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAAGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA GGGTAAACCA
    TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT ATGGAGTGAT
    GCGGCAGCCT TGGAATTATC CAATGGAAGT AATGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GCTACAAAGA GCTCGAGGCC AAGTAAAGCC TTCTACTTCA
    AGTCAGCCTA TACTCTCAGC ACCAGGACCT ACTAAACTCA CTGTAGGGAA TTGGTCACTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCAACAATA
    TTGAAAGAAG ATTTACCTGG TTTTGTGTTT GAGTCTAATA GAGGAACCAA ACATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA GAGAGGCAGA
    AAACTAAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACCATGGC TCGAGATTTA
    TATGATGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTGATGAAG ACAGCTTTAG AAAATCTAAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC ACCATACGAA ATGTGCAGTA GTGGCTTGGT GCAAGCACTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTAGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTC AGTGAAAATG AAGATGATGA AAGTCGACCA
    GCTGTTGCAT TAATCCGAAA GTTAATAGCT GTGCTGGAAT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTGCAGA TACTTACAAG AAGATTAAGA
    TTTCGGTTAG AACGTGCACC TGGTGAAACT GCACTAATAG ACAGAACTGG CAGGATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTAGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ATGATTTCGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCGT TTACTGGATT
    GGGACAAATG CCAAAACTGC ATATGAATGG GTAAATCCAG CTGCATATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ATTAAGTCGT
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCTTGGTT TGCCATAGAT
    CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTA
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCAA AAGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGGAAG
    AATGCTAGTG GACAAACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTC
    AACGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAGGC TAATCTTAGA
    AGACAGAGAC GTCTGGTACG CTCCCAGGTA TTGAAGTACA TGGTTCCAGG GGCTCGTGTT
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACC
    GTCACAGGAG AGCTGCACAA TGGCACAACG CAATCATGGA GCAGCTTGGT GAAAAACAAC
    TGTCCAGACA AGACATCTGC TGCTGCAGGC TCCTCAAGTA GAAAAGGAAG CAGCAGTTCT
    GTGTGTAGCG TGGCCAGTAG CAGCGACATC AGCTTGGGTT CGACCAAAAC GGAACGGAGA
    TCAGAAATTG TAATGGAACA CAGTATAGTT TCAGGAGCTG ATGTCCATGA ACCAATTGTT
    GTTCTTTCAT CTGCTGAAAA CGTCCCTCAA ACAGAAGTAG GGTCATCTTC CAGTGCAAGT
    ACCAGCACCT TAACAGCGGA AACGGGAAGT GAAAATGCTG AAAGAAAGTT AGGCACTGAT
    AGTTCTGTTC GTACTCCTGG GGAGTCTAGT GCAATATCCA TGGGGATTGT TAGTGTTAGT
    TCTCCTGATG TTAGTTCAGT ATCTGAGTTG ACTAATAAAG AAGCAACTTC ACAACGGCCC
    CTTAGCTCTT CAGCGAGTAA CAGACTCTCA GTGAGTTCTT TGTTGGCTGC TGGGGCCCCT
    ATGAGCTCTA GTGCAAGTGT ACCTAACCTG TCCTCAAGAG AAACATCTAG CTTGGAGAGT
    TTTGTAAGGA GAGTGGCAAA CATAGCACGG ACTAATGCCA CGAACAACAT GAATCTAAGC
    CGAAGCAGCA GTGATAACAA CACTAATACT TTGGGGAGGA ATGTGATGAG CACAGCAACT
    TCTCCTCTTA TGGGCGCTCA GAGTTTCCCT AATTTGACCA CACCTGGTAC TACATCAACA
    GTGACTATGT CAACGTCCAG TGTTACCAGT AGTAGCAATG TAGCTACAGC AACAACAGTT
    TTATCAGTTG GTCAGTCATT AAGTAATACT TTAACCACCA GCCTCACATC AACCTCCAGT
    GAGAGTGACA CAGGTCAGGA AGCAGAATAT TCCTTATATG ATTTCCTTGA TAGCTGCCGT
    GCCAGTACTC TGTTAGCTGA GCTAGATGAC GATGAGGACT TGCCAGAACC AGATGAGGAA
    GATGATGAAA ATGAAGATGA CAATCAGGAG GACCAAGAAT ACGAGGAGAT TCTGAGACGT
    CCATCTCTGC AGCGTCGAGC TGGCTCCCGC TCTGATGTGA CACACCATGC TGTCACCTCA
    CAGCTACCAC AGGTCCCTGC TGGAGCAGGG AGCCGACCTA TTGGGGAGCA GGAAGAAGAA
    GAGTATGAAA CTAAAGGGGG ACGCCGGAGA ACATGGGATG ATGATTATGT GCTAAAGCGG
    CAGTTTTCAG CTTTGGTTCC TGCTTTTGAT CCTAGACCTG GTCGTACTAA TGTACAGCAG
    ACAACTGATT TAGAAATTCC GCCCCCAGGA ACACCTCATT CAGAGCTCTT GGAAGAAGTT
    GAATGTACTC CATCACCTCG ATTAGCCCTC ACTTTGAAAG TAACAGGTCT TGGAACAACT
    CGTGAGGTTG AATTACCACT CACTAATTTC AGATCAACCA TCTTTTACTA TGTACAGAAA
    CTGCTTCAGT TGTCCTGTAA TGGCAATGTG AAATCAGATA AACTTAGGCG TATTTGGGAG
    CCAACATACA CAATTATGTA CAGAGAAATG AAGGATTCTG ACAAAGAAAA AGAAAATGGA
    AAAATGGACT TTTGTGAACA TGGAACTAAG TCTGGGTTAA ACCAGGGGGC CATTTCTACT
    CTTCAAAGTA GTGATATTCT TAACTTGACA AAAGAACAAC CTCAGGCCAA AGCAGGCAAT
    GGACAGAATT CTTGTGGAGT AGAAGACGTC CTTCAGCTTC TGCGTATTCT GTATATAGTT
    GCAAGTGACC CTTATTCAAG AATATCCCAA GAAGAAGGTG ATGAGCAGCC TCAGTTTACT
    TTTCCACCAG ATGAATTCAC TAGCAAAAAA ATCACAACAA AAATTTTGCA GCAAATTGAG
    GAACCATTGG CATTGGCAAG TGGCGCTCTT CCAGACTGGT GTGAACAACT AACCAGCAAG
    TGTCCTTTTC TAATTCCGTT TGAAACTAGG CAGCTTTACT TCACATGTAC GGCGTTTGGT
    GCATCCAGAG CAATCGTGTG GTTACAAAAC CGACGTGAGG CCACTGTAGA AAGAACAAGG
    ACCACAAGTA GTGTAAGACG AGATGATCCT GGAGAGTTTC GAGTTGGTCG TCTCAAGCAT
    GAAAGAGTGA AAGTTCCACG TGGTGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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