NLRP3 cDNA ORF clone, Sus scrofa(pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	NLRP3
Entrez Gene ID	100514823
Full Name	NLR family pyrin domain containing 3
Gene Type	protein-coding
Organism	Sus scrofa(pig)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001256770.1	NP_001243699.1	NACHT, LRR and PYD domains-containing protein 3
NM_001256770.2	NP_001243699.1	NACHT, LRR and PYD domains-containing protein 3
XM_021078925.1	XP_020934584.1	NACHT, LRR and PYD domains-containing protein 3 isoform X2
XM_021078922.1	XP_020934581.1	NACHT, LRR and PYD domains-containing protein 3 isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Identification of the Q969R gain-of-function polymorphism in the gene encoding porcine NLRP3 and its distribution in pigs of Asian and European origin.
Tohno M, Shinkai H, Toki D, Okumura N, Tajima K, Uenishi H
Immunogenetics68(9)693-701(2016 10)

The anti-inflammatory effects of baicalin through suppression of NLRP3 inflammasome pathway in LPS-challenged piglet mononuclear phagocytes.
Ye C, Li S, Yao W, Xu L, Qiu Y, Liu Y, Wu Z, Hou Y
Innate immunity22(3)196-204(2016 Apr)

Activation of sterol regulatory element binding protein and NLRP3 inflammasome in atherosclerotic lesion development in diabetic pigs.
Li Y, Xu S, Jiang B, Cohen RA, Zang M
PloS one8(6)e67532(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

identified six synonymous and six nonsynonymous single-nucleotide polymorphisms (SNPs) in the coding sequence of NLRP3 of six breeds of pigs, including major commercial breeds. Among the nonsynonymous SNPs
Title: Identification of the Q969R gain-of-function polymorphism in the gene encoding porcine NLRP3 and its distribution in pigs of Asian and European origin.

this study shows that the anti-inflammatory activity of baicalin could be related to the suppression of NLRP3 inflammasome pathway under LPS stimulation
Title: The anti-inflammatory effects of baicalin through suppression of NLRP3 inflammasome pathway in LPS-challenged piglet mononuclear phagocytes.

dysregulation of SIRT1-AMPK-SREBP and stimulation of NLRP3 inflammasome may contribute to vascular lipid deposition and inflammation in atherosclerosis
Title: Activation of sterol regulatory element binding protein and NLRP3 inflammasome in atherosclerotic lesion development in diabetic pigs.

enhanced expression in gut-associated lymphoid tissues by immunobiotic Lactobacillus strains
Title: Immunobiotic Lactobacillus strains augment NLRP3 expression in newborn and adult porcine gut-associated lymphoid tissues.

The following NLRP3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NLRP3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSe242473	XM_021078922.1 Latest version!	Sus scrofa NLR family pyrin domain containing 3 (NLRP3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OSe242474	XM_021078925.1 Latest version!	Sus scrofa NLR family pyrin domain containing 3 (NLRP3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OSe30559	NM_001256770.2 Latest version!	Sus scrofa NLR family pyrin domain containing 3 (NLRP3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OSe30559	NM_001256770.1	Sus scrofa NLR family pyrin domain containing 3 (NLRP3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NLRP3 ( XM_021078922.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020934581.1 Sus scrofa NLR family pyrin domain containing 3 (NLRP3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSe242473
Clone ID Related Accession (Same CDS sequence)	XM_021078922.1
Accession Version	XM_021078922.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2775bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	NACHT, LRR and PYD domains-containing protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010444.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTTAC TGGGGTACCT TTCCAGAATC TCTATTTGTA AGAAAAAGAA AGATTATTGT 
AAGAAGTACA GAAAGCACGT GAGAAGCAGA TTCCAGTGCA TCAAAGACAG GAATGCACGT 
CTGGGTGAGA GTGTGAACCT CAACAAACGC TTCACCAGGC TGCGTCTCAT CAAGGAACAC 
CGGAGTCAGC AGGAGAGGGA GCATGAGCTC CTTGCCATTG GCAGGACCTC AGCCAAGATG 
CAAGATGGCC CCGTGAGTTC CCTGAACTTG GAATTGCTGT TTGATCCTGA GGACCAACAC 
TCTGAGCCTG TGCACACGGT AGTATTCCAG GGAGCAGCAG GCATTGGGAA AACAATACTG 
GCCAGGAAGA TCATGTTGGA CTGGGCATCA GAGAAACTTT ACCAGGAAAA ATTTGACTAT 
TTGTTTTACA TTCACTGTCG GGAGGTGAGC CTAGGAACTC GGAGGAGCCT GGGAGACCTG 
ATCGCCAGCT GCTGCCCTGG CCCAAACCCA CCCATAGGCA AGATTGTGAG CAAGCCTTCC 
AGGATCCTCT TCCTCATGGA TGGCTTTGAT GAGCTGCAAG GTGCCTTTGA TGAGCGCACA 
GAGGCACTCT GCACAAACTG GCAGAAGGTG GAGCGGGGAG ACATTCTCCT GAGCAGCCTC 
ATCAGAAAAA GACTGCTTCC TGAGGCCTCC CTGCTCATCA CCACAAGACC TGTGGCCCTG 
GAGAAACTTC AGCACTTGCT GGGCCGGGCT CGTCATGTGG AGATTCTGGG TTTCTCAGAG 
GCCAAGAGGA AGGAATATTT CTTTAAGTAT TTTTCAGATG AACAGCAAGC AAGGGAAGCC 
TTCAGGCTGA TTCAGGAGAA TGAGGTCCTG TTCACCATGT GCTTTATTCC CCTGGTCTGC 
TGGATTGTGT GCACTGGGCT GAAACAGCAG ATGGATAGCG GCAAGAGTCT TGCCAGGACA 
TCCAAGACCA CCACTGCCGT GTATATCTTC TTCCTCTCCA CTTTGTTGCA ATCTCAAGAA 
GGGAGCCAGG ACCACCACGT CTCTGCCACC CTCTGGGGTC TCTGCTCACT GGCTGCGGAT 
GGAATCTGGA ATCAGAAAAT CCTGTTTGAG GAGTGTGATC TCAGGAACCA TGGCCTGCAG 
AAGGCAGATG TGTCTGCTTT CCTGAGGATG AACCTATTCC AAAAGGAAGT AGACTGTGAG 
AAATTCTACA GCTTCATTCA CATGACTTTC CAGGAGTTCT TTGCTGCCAT GTACTATCTA 
CTGGAAAAGG AGGATCAGGG GGAGATGAGG AATGTGCCAC GAAGCTGTTT GAAGCTTCCC 
AGTCGAGACG TGACAGTTCT TCTTGAAAAC TATGGCAAAT TTGAAAAGGG GTATCTGATT 
TTTGTTGTCC GTTTCCTCTT TGGCCTTGTA AACCAGGAGA GAACGTCCTA CTTGGAGAAA 
AAACTGAGTT GTAAGATCTC TCAGAAAATC AGGCTAGAGC TGCTTAAATG GATTGAAGCG 
AAAGCCAAGG CCAAGAAGCT ACAGATTGAG CCCAGCCAGC TGGAATTATT TTACTGTTTG 
TACGAAATGC AGGAGGAGGA CTTTGTGCAA AAGGCCATGG GCCATTTCCC TAAAATTGAG 
ATCAATCTCT CCACCAGAAT GGACCATGTA GTTTCTTCTT TTTGTATTGA GAACTGTCGC 
CATGTGGAAT CACTTTCTCT GAGGTTGCTC CATAATTCAC CCAAAGAGGA GGAGGAGGAA 
GAGGAGATAG AGGAGGAGGA TGTTCAACAC TCTGATGTGG ACAATTGTAT CCTCTCTGAG 
TCTCATGTTG CATATTCTCA GCGATTGGTG AACTATCTCA CTTCCAGCTT TTGTAGTGGC 
ATCTTCTCAG TCCTGAGCAA TAACTGGAAT CTCACTGAAT TGAACCTCAG TGGTAACAGC 
CTGGGAGACC CAGGGATGAA GGTGTTATGT GAAACGCTCC AGCAACCTGG CTGTAACATT 
CGAAGATTAT GGTTGGGACA GTGTTGCCTG TCCCATCAGT GCTGCTTCAA CATCTCCTCT 
GTCCTGAGCA ACAACCAGAA GTTGGTGGAA CTGGATCTGA GCCACAATGC CCTGGGAGAC 
TTTGGGATCA GACTTCTGTG TGTGGGACTG AGGCATCTAT TCTGCAAGCT AAAGAAGCTC 
TGGTTGGTCA GTTGCTGTCT CACATCAGCG TGTTGTGAGG ATCTTGCGTC CGTCCTGAGC 
AGCAATCATT CCCTGACCAG ACTCTACTTG GGTGAAAATG CCCTGGGAGA CTCAGGAGTT 
GGAATTTTAT GTGAAAAAGC AAAGCATCCA CAATGTAACC TGCAAAAACT GGGGTTGGTG 
AATTCTGGCC TTACATCAGG TTGTTGTCTA GCTCTATCCT CAGTGCTCAG CACGAACCAG 
AATCTCACTC ACCTCTATCT ACGGGGAAAT GCTCTTGGAG ACATCGGAGT CAAGCAACTC 
TGTGAGGGAC TGTTGCACCC GAACTGCAAG CTTCAAGTGT TGGAATTAGA GAACTGCAGC 
CTCACATCAC ACTGCTGCTG GGATCTTTCC ACACTTCTGA CTTCTAACCA GAGCCTGCGA 
AAGCTGAGCC TAGGCAACAA TGACCTGGGT GATCTGGGGG TCATGCTACT CTGTGAAGTG 
TTGAAACAGC AGGGGTGCCT CCTGAAAAGC CTTAAGTTGT GTGAAATGTA TTTCAATTAT 
GATACAAAAC ATGCATTACA AACACTGCAA GAAGAAAAGC CTGAGTTGAC CATTGTCTTT 
GAGCCTTCCC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020934581.1
CDS	331..3105
Translation	MGLLGYLSRISICKKKKDYCKKYRKHVRSRFQCIKDRNARLGESVNLNKRFTRLRLIKEHRSQQEREHELLAIGRTSAKMQDGPVSSLNLELLFDPEDQHSEPVHTVVFQGAAGIGKTILARKIMLDWASEKLYQEKFDYLFYIHCREVSLGTRRSLGDLIASCCPGPNPPIGKIVSKPSRILFLMDGFDELQGAFDERTEALCTNWQKVERGDILLSSLIRKRLLPEASLLITTRPVALEKLQHLLGRARHVEILGFSEAKRKEYFFKYFSDEQQAREAFRLIQENEVLFTMCFIPLVCWIVCTGLKQQMDSGKSLARTSKTTTAVYIFFLSTLLQSQEGSQDHHVSATLWGLCSLAADGIWNQKILFEECDLRNHGLQKADVSAFLRMNLFQKEVDCEKFYSFIHMTFQEFFAAMYYLLEKEDQGEMRNVPRSCLKLPSRDVTVLLENYGKFEKGYLIFVVRFLFGLVNQERTSYLEKKLSCKISQKIRLELLKWIEAKAKAKKLQIEPSQLELFYCLYEMQEEDFVQKAMGHFPKIEINLSTRMDHVVSSFCIENCRHVESLSLRLLHNSPKEEEEEEEIEEEDVQHSDVDNCILSESHVAYSQRLVNYLTSSFCSGIFSVLSNNWNLTELNLSGNSLGDPGMKVLCETLQQPGCNIRRLWLGQCCLSHQCCFNISSVLSNNQKLVELDLSHNALGDFGIRLLCVGLRHLFCKLKKLWLVSCCLTSACCEDLASVLSSNHSLTRLYLGENALGDSGVGILCEKAKHPQCNLQKLGLVNSGLTSGCCLALSSVLSTNQNLTHLYLRGNALGDIGVKQLCEGLLHPNCKLQVLELENCSLTSHCCWDLSTLLTSNQSLRKLSLGNNDLGDLGVMLLCEVLKQQGCLLKSLKLCEMYFNYDTKHALQTLQEEKPELTIVFEPSQ

Target ORF information:

RefSeq Version	XM_021078922.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa NLR family pyrin domain containing 3 (NLRP3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021078922.1

ORF Insert Sequence:

ATGGGTTTAC TGGGGTACCT TTCCAGAATC TCTATTTGTA AGAAAAAGAA AGATTATTGT 
AAGAAGTACA GAAAGCACGT GAGAAGCAGA TTCCAGTGCA TCAAAGACAG GAATGCACGT 
CTGGGTGAGA GTGTGAACCT CAACAAACGC TTCACCAGGC TGCGTCTCAT CAAGGAACAC 
CGGAGTCAGC AGGAGAGGGA GCATGAGCTC CTTGCCATTG GCAGGACCTC AGCCAAGATG 
CAAGATGGCC CCGTGAGTTC CCTGAACTTG GAATTGCTGT TTGATCCTGA GGACCAACAC 
TCTGAGCCTG TGCACACGGT AGTATTCCAG GGAGCAGCAG GCATTGGGAA AACAATACTG 
GCCAGGAAGA TCATGTTGGA CTGGGCATCA GAGAAACTTT ACCAGGAAAA ATTTGACTAT 
TTGTTTTACA TTCACTGTCG GGAGGTGAGC CTAGGAACTC GGAGGAGCCT GGGAGACCTG 
ATCGCCAGCT GCTGCCCTGG CCCAAACCCA CCCATAGGCA AGATTGTGAG CAAGCCTTCC 
AGGATCCTCT TCCTCATGGA TGGCTTTGAT GAGCTGCAAG GTGCCTTTGA TGAGCGCACA 
GAGGCACTCT GCACAAACTG GCAGAAGGTG GAGCGGGGAG ACATTCTCCT GAGCAGCCTC 
ATCAGAAAAA GACTGCTTCC TGAGGCCTCC CTGCTCATCA CCACAAGACC TGTGGCCCTG 
GAGAAACTTC AGCACTTGCT GGGCCGGGCT CGTCATGTGG AGATTCTGGG TTTCTCAGAG 
GCCAAGAGGA AGGAATATTT CTTTAAGTAT TTTTCAGATG AACAGCAAGC AAGGGAAGCC 
TTCAGGCTGA TTCAGGAGAA TGAGGTCCTG TTCACCATGT GCTTTATTCC CCTGGTCTGC 
TGGATTGTGT GCACTGGGCT GAAACAGCAG ATGGATAGCG GCAAGAGTCT TGCCAGGACA 
TCCAAGACCA CCACTGCCGT GTATATCTTC TTCCTCTCCA CTTTGTTGCA ATCTCAAGAA 
GGGAGCCAGG ACCACCACGT CTCTGCCACC CTCTGGGGTC TCTGCTCACT GGCTGCGGAT 
GGAATCTGGA ATCAGAAAAT CCTGTTTGAG GAGTGTGATC TCAGGAACCA TGGCCTGCAG 
AAGGCAGATG TGTCTGCTTT CCTGAGGATG AACCTATTCC AAAAGGAAGT AGACTGTGAG 
AAATTCTACA GCTTCATTCA CATGACTTTC CAGGAGTTCT TTGCTGCCAT GTACTATCTA 
CTGGAAAAGG AGGATCAGGG GGAGATGAGG AATGTGCCAC GAAGCTGTTT GAAGCTTCCC 
AGTCGAGACG TGACAGTTCT TCTTGAAAAC TATGGCAAAT TTGAAAAGGG GTATCTGATT 
TTTGTTGTCC GTTTCCTCTT TGGCCTTGTA AACCAGGAGA GAACGTCCTA CTTGGAGAAA 
AAACTGAGTT GTAAGATCTC TCAGAAAATC AGGCTAGAGC TGCTTAAATG GATTGAAGCG 
AAAGCCAAGG CCAAGAAGCT ACAGATTGAG CCCAGCCAGC TGGAATTATT TTACTGTTTG 
TACGAAATGC AGGAGGAGGA CTTTGTGCAA AAGGCCATGG GCCATTTCCC TAAAATTGAG 
ATCAATCTCT CCACCAGAAT GGACCATGTA GTTTCTTCTT TTTGTATTGA GAACTGTCGC 
CATGTGGAAT CACTTTCTCT GAGGTTGCTC CATAATTCAC CCAAAGAGGA GGAGGAGGAA 
GAGGAGATAG AGGAGGAGGA TGTTCAACAC TCTGATGTGG ACAATTGTAT CCTCTCTGAG 
TCTCATGTTG CATATTCTCA GCGATTGGTG AACTATCTCA CTTCCAGCTT TTGTAGTGGC 
ATCTTCTCAG TCCTGAGCAA TAACTGGAAT CTCACTGAAT TGAACCTCAG TGGTAACAGC 
CTGGGAGACC CAGGGATGAA GGTGTTATGT GAAACGCTCC AGCAACCTGG CTGTAACATT 
CGAAGATTAT GGTTGGGACA GTGTTGCCTG TCCCATCAGT GCTGCTTCAA CATCTCCTCT 
GTCCTGAGCA ACAACCAGAA GTTGGTGGAA CTGGATCTGA GCCACAATGC CCTGGGAGAC 
TTTGGGATCA GACTTCTGTG TGTGGGACTG AGGCATCTAT TCTGCAAGCT AAAGAAGCTC 
TGGTTGGTCA GTTGCTGTCT CACATCAGCG TGTTGTGAGG ATCTTGCGTC CGTCCTGAGC 
AGCAATCATT CCCTGACCAG ACTCTACTTG GGTGAAAATG CCCTGGGAGA CTCAGGAGTT 
GGAATTTTAT GTGAAAAAGC AAAGCATCCA CAATGTAACC TGCAAAAACT GGGGTTGGTG 
AATTCTGGCC TTACATCAGG TTGTTGTCTA GCTCTATCCT CAGTGCTCAG CACGAACCAG 
AATCTCACTC ACCTCTATCT ACGGGGAAAT GCTCTTGGAG ACATCGGAGT CAAGCAACTC 
TGTGAGGGAC TGTTGCACCC GAACTGCAAG CTTCAAGTGT TGGAATTAGA GAACTGCAGC 
CTCACATCAC ACTGCTGCTG GGATCTTTCC ACACTTCTGA CTTCTAACCA GAGCCTGCGA 
AAGCTGAGCC TAGGCAACAA TGACCTGGGT GATCTGGGGG TCATGCTACT CTGTGAAGTG 
TTGAAACAGC AGGGGTGCCT CCTGAAAAGC CTTAAGTTGT GTGAAATGTA TTTCAATTAT 
GATACAAAAC ATGCATTACA AACACTGCAA GAAGAAAAGC CTGAGTTGAC CATTGTCTTT 
GAGCCTTCCC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP3 ( XM_021078925.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020934584.1 Sus scrofa NLR family pyrin domain containing 3 (NLRP3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSe242474
Clone ID Related Accession (Same CDS sequence)	XM_021078925.1
Accession Version	XM_021078925.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2679bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	NACHT, LRR and PYD domains-containing protein 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010444.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATGG CAAGCGTCCG CTGCAAGCTG GCTCGTTACC TGGAGGACCT AGAAGATGTA 
GATTTTAAGA AATTCAAGAT GCACTTAGAA GACTATCCTT CTCAGAAGGG CTGCATCTCT 
CTCCCTCGGG GCCAGACAGA AAAAGCAGAT CATGTGGATC TAGCCACTCT GATGATAGAT 
TTCAATGGAG AGGAGAAGGC ATGGGCCATG GCCACATGGA TTTTTGCTGC GATCAACAGG 
AGAGACCTTT ATGAGAAAGC TAAGAGGGAT GAGCCAGAAT GGGACAATGC AAATCTTTCT 
GTGATAAGCC AGGAAGAAAG CCTTGAAGAG GAATGGATGG GTTTACTGGG GTACCTTTCC 
AGAATCTCTA TTTGTAAGAA AAAGAAAGAT TATTGTAAGA AGTACAGAAA GCACGTGAGA 
AGCAGATTCC AGTGCATCAA AGACAGGAAT GCACGTCTGG GTGAGAGTGT GAACCTCAAC 
AAACGCTTCA CCAGGCTGCG TCTCATCAAG GAACACCGGA GTCAGCAGGA GAGGGAGCAT 
GAGCTCCTTG CCATTGGCAG GACCTCAGCC AAGATGCAAG ATGGCCCCGT GAGTTCCCTG 
AACTTGGAAT TGCTGTTTGA TCCTGAGGAC CAACACTCTG AGCCTGTGCA CACGGTAGTA 
TTCCAGGGAG CAGCAGGCAT TGGGAAAACA ATACTGGCCA GGAAGATCAT GTTGGACTGG 
GCATCAGAGA AACTTTACCA GGAAAAATTT GACTATTTGT TTTACATTCA CTGTCGGGAG 
GTGAGCCTAG GAACTCGGAG GAGCCTGGGA GACCTGATCG CCAGCTGCTG CCCTGGCCCA 
AACCCACCCA TAGGCAAGAT TGTGAGCAAG CCTTCCAGGA TCCTCTTCCT CATGGATGGC 
TTTGATGAGC TGCAAGGTGC CTTTGATGAG CGCACAGAGG CACTCTGCAC AAACTGGCAG 
AAGGTGGAGC GGGGAGACAT TCTCCTGAGC AGCCTCATCA GAAAAAGACT GCTTCCTGAG 
GCCTCCCTGC TCATCACCAC AAGACCTGTG GCCCTGGAGA AACTTCAGCA CTTGCTGGGC 
CGGGCTCGTC ATGTGGAGAT TCTGGGTTTC TCAGAGGCCA AGAGGAAGGA ATATTTCTTT 
AAGTATTTTT CAGATGAACA GCAAGCAAGG GAAGCCTTCA GGCTGATTCA GGAGAATGAG 
GTCCTGTTCA CCATGTGCTT TATTCCCCTG GTCTGCTGGA TTGTGTGCAC TGGGCTGAAA 
CAGCAGATGG ATAGCGGCAA GAGTCTTGCC AGGACATCCA AGACCACCAC TGCCGTGTAT 
ATCTTCTTCC TCTCCACTTT GTTGCAATCT CAAGAAGGGA GCCAGGACCA CCACGTCTCT 
GCCACCCTCT GGGGTCTCTG CTCACTGGCT GCGGATGGAA TCTGGAATCA GAAAATCCTG 
TTTGAGGAGT GTGATCTCAG GAACCATGGC CTGCAGAAGG CAGATGTGTC TGCTTTCCTG 
AGGATGAACC TATTCCAAAA GGAAGTAGAC TGTGAGAAAT TCTACAGCTT CATTCACATG 
ACTTTCCAGG AGTTCTTTGC TGCCATGTAC TATCTACTGG AAAAGGAGGA TCAGGGGGAG 
ATGAGGAATG TGCCACGAAG CTGTTTGAAG CTTCCCAGTC GAGACGTGAC AGTTCTTCTT 
GAAAACTATG GCAAATTTGA AAAGGGGTAT CTGATTTTTG TTGTCCGTTT CCTCTTTGGC 
CTTGTAAACC AGGAGAGAAC GTCCTACTTG GAGAAAAAAC TGAGTTGTAA GATCTCTCAG 
AAAATCAGGC TAGAGCTGCT TAAATGGATT GAAGCGAAAG CCAAGGCCAA GAAGCTACAG 
ATTGAGCCCA GCCAGCTGGA ATTATTTTAC TGTTTGTACG AAATGCAGGA GGAGGACTTT 
GTGCAAAAGG CCATGGGCCA TTTCCCTAAA ATTGAGATCA ATCTCTCCAC CAGAATGGAC 
CATGTAGTTT CTTCTTTTTG TATTGAGAAC TGTCGCCATG TGGAATCACT TTCTCTGAGG 
TTGCTCCATA ATTCACCCAA AGAGGAGGAG GAGGAAGAGG AGATAGAGGA GGAGGATGTT 
CAACACTCTG ATGTGGACAA TTGTATCCTC TCTGAGTCTC ATGTTGCATA TTCTCAGCGA 
TTGGTGAACT ATCTCACTTC CAGCTTTTGT AGTGGCATCT TCTCAGTCCT GAGCAATAAC 
TGGAATCTCA CTGAATTGAA CCTCAGTGGT AACAGCCTGG GAGACCCAGG GATGAAGGTG 
TTATGTGAAA CGCTCCAGCA ACCTGGCTGT AACATTCGAA GATTATGGTT GGGACAGTGT 
TGCCTGTCCC ATCAGTGCTG CTTCAACATC TCCTCTGTCC TGAGCAACAA CCAGAAGTTG 
GTGGAACTGG ATCTGAGCCA CAATGCCCTG GGAGACTTTG GGATCAGACT TCTGTGTGTG 
GGACTGAGGC ATCTATTCTG CAAGCTAAAG AAGCTCTGGT TGGTCAGTTG CTGTCTCACA 
TCAGCGTGTT GTGAGGATCT TGCGTCCGTC CTGAGCAGCA ATCATTCCCT GACCAGACTC 
TACTTGGGTG AAAATGCCCT GGGAGACTCA GGAGTTGGAA TTTTATGTGA AAAAGCAAAG 
CATCCACAAT GTAACCTGCA AAAACTGGGA TTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020934584.1
CDS	316..2994
Translation	MSMASVRCKLARYLEDLEDVDFKKFKMHLEDYPSQKGCISLPRGQTEKADHVDLATLMIDFNGEEKAWAMATWIFAAINRRDLYEKAKRDEPEWDNANLSVISQEESLEEEWMGLLGYLSRISICKKKKDYCKKYRKHVRSRFQCIKDRNARLGESVNLNKRFTRLRLIKEHRSQQEREHELLAIGRTSAKMQDGPVSSLNLELLFDPEDQHSEPVHTVVFQGAAGIGKTILARKIMLDWASEKLYQEKFDYLFYIHCREVSLGTRRSLGDLIASCCPGPNPPIGKIVSKPSRILFLMDGFDELQGAFDERTEALCTNWQKVERGDILLSSLIRKRLLPEASLLITTRPVALEKLQHLLGRARHVEILGFSEAKRKEYFFKYFSDEQQAREAFRLIQENEVLFTMCFIPLVCWIVCTGLKQQMDSGKSLARTSKTTTAVYIFFLSTLLQSQEGSQDHHVSATLWGLCSLAADGIWNQKILFEECDLRNHGLQKADVSAFLRMNLFQKEVDCEKFYSFIHMTFQEFFAAMYYLLEKEDQGEMRNVPRSCLKLPSRDVTVLLENYGKFEKGYLIFVVRFLFGLVNQERTSYLEKKLSCKISQKIRLELLKWIEAKAKAKKLQIEPSQLELFYCLYEMQEEDFVQKAMGHFPKIEINLSTRMDHVVSSFCIENCRHVESLSLRLLHNSPKEEEEEEEIEEEDVQHSDVDNCILSESHVAYSQRLVNYLTSSFCSGIFSVLSNNWNLTELNLSGNSLGDPGMKVLCETLQQPGCNIRRLWLGQCCLSHQCCFNISSVLSNNQKLVELDLSHNALGDFGIRLLCVGLRHLFCKLKKLWLVSCCLTSACCEDLASVLSSNHSLTRLYLGENALGDSGVGILCEKAKHPQCNLQKLGFC

Target ORF information:

RefSeq Version	XM_021078925.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa NLR family pyrin domain containing 3 (NLRP3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021078925.1

ORF Insert Sequence:

ATGAGCATGG CAAGCGTCCG CTGCAAGCTG GCTCGTTACC TGGAGGACCT AGAAGATGTA 
GATTTTAAGA AATTCAAGAT GCACTTAGAA GACTATCCTT CTCAGAAGGG CTGCATCTCT 
CTCCCTCGGG GCCAGACAGA AAAAGCAGAT CATGTGGATC TAGCCACTCT GATGATAGAT 
TTCAATGGAG AGGAGAAGGC ATGGGCCATG GCCACATGGA TTTTTGCTGC GATCAACAGG 
AGAGACCTTT ATGAGAAAGC TAAGAGGGAT GAGCCAGAAT GGGACAATGC AAATCTTTCT 
GTGATAAGCC AGGAAGAAAG CCTTGAAGAG GAATGGATGG GTTTACTGGG GTACCTTTCC 
AGAATCTCTA TTTGTAAGAA AAAGAAAGAT TATTGTAAGA AGTACAGAAA GCACGTGAGA 
AGCAGATTCC AGTGCATCAA AGACAGGAAT GCACGTCTGG GTGAGAGTGT GAACCTCAAC 
AAACGCTTCA CCAGGCTGCG TCTCATCAAG GAACACCGGA GTCAGCAGGA GAGGGAGCAT 
GAGCTCCTTG CCATTGGCAG GACCTCAGCC AAGATGCAAG ATGGCCCCGT GAGTTCCCTG 
AACTTGGAAT TGCTGTTTGA TCCTGAGGAC CAACACTCTG AGCCTGTGCA CACGGTAGTA 
TTCCAGGGAG CAGCAGGCAT TGGGAAAACA ATACTGGCCA GGAAGATCAT GTTGGACTGG 
GCATCAGAGA AACTTTACCA GGAAAAATTT GACTATTTGT TTTACATTCA CTGTCGGGAG 
GTGAGCCTAG GAACTCGGAG GAGCCTGGGA GACCTGATCG CCAGCTGCTG CCCTGGCCCA 
AACCCACCCA TAGGCAAGAT TGTGAGCAAG CCTTCCAGGA TCCTCTTCCT CATGGATGGC 
TTTGATGAGC TGCAAGGTGC CTTTGATGAG CGCACAGAGG CACTCTGCAC AAACTGGCAG 
AAGGTGGAGC GGGGAGACAT TCTCCTGAGC AGCCTCATCA GAAAAAGACT GCTTCCTGAG 
GCCTCCCTGC TCATCACCAC AAGACCTGTG GCCCTGGAGA AACTTCAGCA CTTGCTGGGC 
CGGGCTCGTC ATGTGGAGAT TCTGGGTTTC TCAGAGGCCA AGAGGAAGGA ATATTTCTTT 
AAGTATTTTT CAGATGAACA GCAAGCAAGG GAAGCCTTCA GGCTGATTCA GGAGAATGAG 
GTCCTGTTCA CCATGTGCTT TATTCCCCTG GTCTGCTGGA TTGTGTGCAC TGGGCTGAAA 
CAGCAGATGG ATAGCGGCAA GAGTCTTGCC AGGACATCCA AGACCACCAC TGCCGTGTAT 
ATCTTCTTCC TCTCCACTTT GTTGCAATCT CAAGAAGGGA GCCAGGACCA CCACGTCTCT 
GCCACCCTCT GGGGTCTCTG CTCACTGGCT GCGGATGGAA TCTGGAATCA GAAAATCCTG 
TTTGAGGAGT GTGATCTCAG GAACCATGGC CTGCAGAAGG CAGATGTGTC TGCTTTCCTG 
AGGATGAACC TATTCCAAAA GGAAGTAGAC TGTGAGAAAT TCTACAGCTT CATTCACATG 
ACTTTCCAGG AGTTCTTTGC TGCCATGTAC TATCTACTGG AAAAGGAGGA TCAGGGGGAG 
ATGAGGAATG TGCCACGAAG CTGTTTGAAG CTTCCCAGTC GAGACGTGAC AGTTCTTCTT 
GAAAACTATG GCAAATTTGA AAAGGGGTAT CTGATTTTTG TTGTCCGTTT CCTCTTTGGC 
CTTGTAAACC AGGAGAGAAC GTCCTACTTG GAGAAAAAAC TGAGTTGTAA GATCTCTCAG 
AAAATCAGGC TAGAGCTGCT TAAATGGATT GAAGCGAAAG CCAAGGCCAA GAAGCTACAG 
ATTGAGCCCA GCCAGCTGGA ATTATTTTAC TGTTTGTACG AAATGCAGGA GGAGGACTTT 
GTGCAAAAGG CCATGGGCCA TTTCCCTAAA ATTGAGATCA ATCTCTCCAC CAGAATGGAC 
CATGTAGTTT CTTCTTTTTG TATTGAGAAC TGTCGCCATG TGGAATCACT TTCTCTGAGG 
TTGCTCCATA ATTCACCCAA AGAGGAGGAG GAGGAAGAGG AGATAGAGGA GGAGGATGTT 
CAACACTCTG ATGTGGACAA TTGTATCCTC TCTGAGTCTC ATGTTGCATA TTCTCAGCGA 
TTGGTGAACT ATCTCACTTC CAGCTTTTGT AGTGGCATCT TCTCAGTCCT GAGCAATAAC 
TGGAATCTCA CTGAATTGAA CCTCAGTGGT AACAGCCTGG GAGACCCAGG GATGAAGGTG 
TTATGTGAAA CGCTCCAGCA ACCTGGCTGT AACATTCGAA GATTATGGTT GGGACAGTGT 
TGCCTGTCCC ATCAGTGCTG CTTCAACATC TCCTCTGTCC TGAGCAACAA CCAGAAGTTG 
GTGGAACTGG ATCTGAGCCA CAATGCCCTG GGAGACTTTG GGATCAGACT TCTGTGTGTG 
GGACTGAGGC ATCTATTCTG CAAGCTAAAG AAGCTCTGGT TGGTCAGTTG CTGTCTCACA 
TCAGCGTGTT GTGAGGATCT TGCGTCCGTC CTGAGCAGCA ATCATTCCCT GACCAGACTC 
TACTTGGGTG AAAATGCCCT GGGAGACTCA GGAGTTGGAA TTTTATGTGA AAAAGCAAAG 
CATCCACAAT GTAACCTGCA AAAACTGGGA TTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP3 ( NM_001256770.1 ) cDNA ORF clone, Sus scrofa(pig) -> NP_001243699.1 Sus scrofa NLR family pyrin domain containing 3 (NLRP3), mRNA.

Price & Availability↑

CloneID	OSe30559
Clone ID Related Accession (Same CDS sequence)	NM_001256770.1 , NM_001256770.2
Accession Version	NM_001256770.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3111bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-25
Organism	Sus scrofa(Pig)
Product	NACHT, LRR and PYD domains-containing protein 3
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from JQ219660.1. On Feb 20, 2012 this sequence version replaced XM_003123558.1. ##Evidence-Data-START## Transcript exon combination :: JQ219660.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1876826, SAMEA1876828 [ECO:0000350] ##Evidence-Data-END##

ATGAGCATGG CAAGCGTCCG CTGCAAGCTG GCTCGTTACC TGGAGGACCT AGAAGATGTA 
GATTTTAAGA AATTCAAGAT GCACTTAGAA GACTATCCTT CTCAGAAGGG CTGCATCTCT 
CTCCCTCGGG GCCAGACAGA AAAAGCAGAT CATGTGGATC TAGCCACTCT GATGATAGAT 
TTCAATGGAG AGGAGAAGGC ATGGGCCATG GCCACATGGA TTTTTGCTGC GATCAACAGG 
AGAGACCTTT ATGAGAAAGC TAAGAGGGAT GAGCCAGAAT GGGACAATGC AAATCTTTCT 
GTGATAAGCC AGGAAGAAAG CCTTGAAGAG GAATGGATGG GTTTACTGGG GTACCTTTCC 
AGAATCTCTA TTTGTAAGAA AAAGAAAGAT TATTGTAAGA AGTACAGAAA GCACGTGAGA 
AGCAGATTCC AGTGCATCAA AGACAGGAAT GCACGTCTGG GTGAGAGTGT GAACCTCAAC 
AAACGCTTCA CCAGGCTGCG TCTCATCAAG GAACACCGGA GTCAGCAGGA GAGGGAGCAT 
GAGCTCCTTG CCATTGGCAG GACCTCAGCC AAGATGCAAG ATGGCCCCGT GAGTTCCCTG 
AACTTGGAAT TGCTGTTTGA TCCTGAGGAC CAACACTCTG AGCCTGTGCA CACGGTAGTA 
TTCCAGGGAG CAGCAGGCAT TGGGAAAACA ATACTGGCCA GGAAGATCAT GTTGGACTGG 
GCATCAGAGA AACTTTACCA GGAAAAATTT GACTATTTGT TTTACATTCA CTGTCGGGAG 
GTGAGCCTAG GAACTCGGAG GAGCCTGGGA GACCTGATCG CCAGCTGCTG CCCTGGCCCA 
AACCCACCCA TAGGCAAGAT TGTGAGCAAG CCTTCCAGGA TCCTCTTCCT CATGGATGGC 
TTTGATGAGC TGCAAGGTGC CTTTGATGAG CGCACAGAGG CACTCTGCAC AAACTGGCAG 
AAGGTGGAGC GGGGAGACAT TCTCCTGAGC AGCCTCATCA GAAAAAGACT GCTTCCTGAG 
GCCTCCCTGC TCATCACCAC AAGACCTGTG GCCCTGGAGA AACTTCAGCA CTTGCTGGGC 
CGGGCTCGTC ATGTGGAGAT TCTGGGTTTC TCAGAGGCCA AGAGGAAGGA ATATTTCTTT 
AAGTATTTTT CAGATGAACA GCAAGCAAGG GAAGCCTTCA GGCTGATTCA GGAGAATGAG 
GTCCTGTTCA CCATGTGCTT TATTCCCCTG GTCTGCTGGA TTGTGTGCAC TGGGCTGAAA 
CAGCAGATGG ATAGCGGCAA GAGTCTTGCC AGGACATCCA AGACCACCAC TGCCGTGTAT 
ATCTTCTTCC TCTCCACTTT GTTGCAATCT CAAGAAGGGA GCCAGGACCA CCACGTCTCT 
GCCACCCTCT GGGGTCTCTG CTCACTGGCT GCGGATGGAA TCTGGAATCA GAAAATCCTG 
TTTGAGGAGT GTGATCTCAG GAACCATGGC CTGCAGAAGG CAGATGTGTC TGCTTTCCTG 
AGGATGAACC TATTCCAAAA GGAAGTAGAC TGTGAGAAAT TCTACAGCTT CATTCACATG 
ACTTTCCAGG AGTTCTTTGC TGCCATGTAC TATCTACTGG AAAAGGAGGA TCAGGGGGAG 
ATGAGGAATG TGCCACGAAG CTGTTTGAAG CTTCCCAGTC GAGACGTGAC AGTTCTTCTT 
GAAAACTATG GCAAATTTGA AAAGGGGTAT CTGATTTTTG TTGTCCGTTT CCTCTTTGGC 
CTTGTAAACC AGGAGAGAAC GTCCTACTTG GAGAAAAAAC TGAGTTGTAA GATCTCTCAG 
AAAATCAGGC TAGAGCTGCT TAAATGGATT GAAGCGAAAG CCAAGGCCAA GAAGCTACAG 
ATTGAGCCCA GCCAGCTGGA ATTATTTTAC TGTTTGTACG AAATGCAGGA GGAGGACTTT 
GTGCAAAAGG CCATGGGCCA TTTCCCTAAA ATTGAGATCA ATCTCTCCAC CAGAATGGAC 
CATGTAGTTT CTTCTTTTTG TATTGAGAAC TGTCGCCATG TGGAATCACT TTCTCTGAGG 
TTGCTCCATA ATTCACCCAA AGAGGAGGAG GAGGAAGAGG AGATAGAGGA GGAGGATGTT 
CAACACTCTG ATGTGGACAA TTGTATCCTC TCTGAGTCTC ATGTTGCATA TTCTCAGCGA 
TTGGTGAACT ATCTCACTTC CAGCTTTTGT AGTGGCATCT TCTCAGTCCT GAGCAATAAC 
TGGAATCTCA CTGAATTGAA CCTCAGTGGT AACAGCCTGG GAGACCCAGG GATGAAGGTG 
TTATGTGAAA CGCTCCAGCA ACCTGGCTGT AACATTCGAA GATTATGGTT GGGACAGTGT 
TGCCTGTCCC ATCAGTGCTG CTTCAACATC TCCTCTGTCC TGAGCAACAA CCAGAAGTTG 
GTGGAACTGG ATCTGAGCCA CAATGCCCTG GGAGACTTTG GGATCAGACT TTTGTGTGTG 
GGACTGAGGC ATCTATTCTG CAAGCTAAAG AAGCTCTGGT TGGTCAGTTG CTGTCTCACA 
TCAGCGTGTT GTGAGGATCT TGCGTCCGTC CTGAGCAGCA ATCATTCCCT GACCAGACTC 
TACTTGGGTG AAAATGCCCT GGGAGACTCA GGAGTTGGAA TTTTATGTGA AAAAGCAAAG 
CATCCACAAT GTAACCTGCA AAAACTGGGG TTGGTGAATT CTGGCCTTAC ATCAGGTTGT 
TGTCTAGCTC TATCCTCAGT GCTCAGCACG AACCAGAATC TCACTCACCT CTATCTACGG 
GGAAATGCTC TTGGAGACAT CGGAGTCAAG CAACTCTGTG AGGGACTGTT GCACCCGAAC 
TGCAAGCTTC AAGTGTTGGA ATTAGAGAAC TGCAGCCTCA CATCACACTG CTGCTGGGAT 
CTTTCCACAC TTCTGACTTC TAACCAGAGC CTGCGAAAGC TGAGCCTAGG CAACAATGAC 
CTGGGTGATC TGGGGGTCAT GCTACTCTGT GAAGTGTTGA AACAGCAGGG GTGCCTCCTG 
AAAAGCCTTA AGTTGTGTGA AATGTATTTC AATTATGATA CAAAACATGC ATTACAAACA 
CTGCAAGAAG AAAAGCCTGA GTTGACCATT GTCTTTGAGC CTTCCCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243699.1
CDS	137..3247
Translation	MSMASVRCKLARYLEDLEDVDFKKFKMHLEDYPSQKGCISLPRGQTEKADHVDLATLMIDFNGEEKAWAMATWIFAAINRRDLYEKAKRDEPEWDNANLSVISQEESLEEEWMGLLGYLSRISICKKKKDYCKKYRKHVRSRFQCIKDRNARLGESVNLNKRFTRLRLIKEHRSQQEREHELLAIGRTSAKMQDGPVSSLNLELLFDPEDQHSEPVHTVVFQGAAGIGKTILARKIMLDWASEKLYQEKFDYLFYIHCREVSLGTRRSLGDLIASCCPGPNPPIGKIVSKPSRILFLMDGFDELQGAFDERTEALCTNWQKVERGDILLSSLIRKRLLPEASLLITTRPVALEKLQHLLGRARHVEILGFSEAKRKEYFFKYFSDEQQAREAFRLIQENEVLFTMCFIPLVCWIVCTGLKQQMDSGKSLARTSKTTTAVYIFFLSTLLQSQEGSQDHHVSATLWGLCSLAADGIWNQKILFEECDLRNHGLQKADVSAFLRMNLFQKEVDCEKFYSFIHMTFQEFFAAMYYLLEKEDQGEMRNVPRSCLKLPSRDVTVLLENYGKFEKGYLIFVVRFLFGLVNQERTSYLEKKLSCKISQKIRLELLKWIEAKAKAKKLQIEPSQLELFYCLYEMQEEDFVQKAMGHFPKIEINLSTRMDHVVSSFCIENCRHVESLSLRLLHNSPKEEEEEEEIEEEDVQHSDVDNCILSESHVAYSQRLVNYLTSSFCSGIFSVLSNNWNLTELNLSGNSLGDPGMKVLCETLQQPGCNIRRLWLGQCCLSHQCCFNISSVLSNNQKLVELDLSHNALGDFGIRLLCVGLRHLFCKLKKLWLVSCCLTSACCEDLASVLSSNHSLTRLYLGENALGDSGVGILCEKAKHPQCNLQKLGLVNSGLTSGCCLALSSVLSTNQNLTHLYLRGNALGDIGVKQLCEGLLHPNCKLQVLELENCSLTSHCCWDLSTLLTSNQSLRKLSLGNNDLGDLGVMLLCEVLKQQGCLLKSLKLCEMYFNYDTKHALQTLQEEKPELTIVFEPSQ

Target ORF information:

RefSeq Version	NM_001256770.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa NLR family pyrin domain containing 3 (NLRP3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256770.1

ORF Insert Sequence:

ATGAGCATGG CAAGCGTCCG CTGCAAGCTG GCTCGTTACC TGGAGGACCT AGAAGATGTA 
GATTTTAAGA AATTCAAGAT GCACTTAGAA GACTATCCTT CTCAGAAGGG CTGCATCTCT 
CTCCCTCGGG GCCAGACAGA AAAAGCAGAT CATGTGGATC TAGCCACTCT GATGATAGAT 
TTCAATGGAG AGGAGAAGGC ATGGGCCATG GCCACATGGA TTTTTGCTGC GATCAACAGG 
AGAGACCTTT ATGAGAAAGC TAAGAGGGAT GAGCCAGAAT GGGACAATGC AAATCTTTCT 
GTGATAAGCC AGGAAGAAAG CCTTGAAGAG GAATGGATGG GTTTACTGGG GTACCTTTCC 
AGAATCTCTA TTTGTAAGAA AAAGAAAGAT TATTGTAAGA AGTACAGAAA GCACGTGAGA 
AGCAGATTCC AGTGCATCAA AGACAGGAAT GCACGTCTGG GTGAGAGTGT GAACCTCAAC 
AAACGCTTCA CCAGGCTGCG TCTCATCAAG GAACACCGGA GTCAGCAGGA GAGGGAGCAT 
GAGCTCCTTG CCATTGGCAG GACCTCAGCC AAGATGCAAG ATGGCCCCGT GAGTTCCCTG 
AACTTGGAAT TGCTGTTTGA TCCTGAGGAC CAACACTCTG AGCCTGTGCA CACGGTAGTA 
TTCCAGGGAG CAGCAGGCAT TGGGAAAACA ATACTGGCCA GGAAGATCAT GTTGGACTGG 
GCATCAGAGA AACTTTACCA GGAAAAATTT GACTATTTGT TTTACATTCA CTGTCGGGAG 
GTGAGCCTAG GAACTCGGAG GAGCCTGGGA GACCTGATCG CCAGCTGCTG CCCTGGCCCA 
AACCCACCCA TAGGCAAGAT TGTGAGCAAG CCTTCCAGGA TCCTCTTCCT CATGGATGGC 
TTTGATGAGC TGCAAGGTGC CTTTGATGAG CGCACAGAGG CACTCTGCAC AAACTGGCAG 
AAGGTGGAGC GGGGAGACAT TCTCCTGAGC AGCCTCATCA GAAAAAGACT GCTTCCTGAG 
GCCTCCCTGC TCATCACCAC AAGACCTGTG GCCCTGGAGA AACTTCAGCA CTTGCTGGGC 
CGGGCTCGTC ATGTGGAGAT TCTGGGTTTC TCAGAGGCCA AGAGGAAGGA ATATTTCTTT 
AAGTATTTTT CAGATGAACA GCAAGCAAGG GAAGCCTTCA GGCTGATTCA GGAGAATGAG 
GTCCTGTTCA CCATGTGCTT TATTCCCCTG GTCTGCTGGA TTGTGTGCAC TGGGCTGAAA 
CAGCAGATGG ATAGCGGCAA GAGTCTTGCC AGGACATCCA AGACCACCAC TGCCGTGTAT 
ATCTTCTTCC TCTCCACTTT GTTGCAATCT CAAGAAGGGA GCCAGGACCA CCACGTCTCT 
GCCACCCTCT GGGGTCTCTG CTCACTGGCT GCGGATGGAA TCTGGAATCA GAAAATCCTG 
TTTGAGGAGT GTGATCTCAG GAACCATGGC CTGCAGAAGG CAGATGTGTC TGCTTTCCTG 
AGGATGAACC TATTCCAAAA GGAAGTAGAC TGTGAGAAAT TCTACAGCTT CATTCACATG 
ACTTTCCAGG AGTTCTTTGC TGCCATGTAC TATCTACTGG AAAAGGAGGA TCAGGGGGAG 
ATGAGGAATG TGCCACGAAG CTGTTTGAAG CTTCCCAGTC GAGACGTGAC AGTTCTTCTT 
GAAAACTATG GCAAATTTGA AAAGGGGTAT CTGATTTTTG TTGTCCGTTT CCTCTTTGGC 
CTTGTAAACC AGGAGAGAAC GTCCTACTTG GAGAAAAAAC TGAGTTGTAA GATCTCTCAG 
AAAATCAGGC TAGAGCTGCT TAAATGGATT GAAGCGAAAG CCAAGGCCAA GAAGCTACAG 
ATTGAGCCCA GCCAGCTGGA ATTATTTTAC TGTTTGTACG AAATGCAGGA GGAGGACTTT 
GTGCAAAAGG CCATGGGCCA TTTCCCTAAA ATTGAGATCA ATCTCTCCAC CAGAATGGAC 
CATGTAGTTT CTTCTTTTTG TATTGAGAAC TGTCGCCATG TGGAATCACT TTCTCTGAGG 
TTGCTCCATA ATTCACCCAA AGAGGAGGAG GAGGAAGAGG AGATAGAGGA GGAGGATGTT 
CAACACTCTG ATGTGGACAA TTGTATCCTC TCTGAGTCTC ATGTTGCATA TTCTCAGCGA 
TTGGTGAACT ATCTCACTTC CAGCTTTTGT AGTGGCATCT TCTCAGTCCT GAGCAATAAC 
TGGAATCTCA CTGAATTGAA CCTCAGTGGT AACAGCCTGG GAGACCCAGG GATGAAGGTG 
TTATGTGAAA CGCTCCAGCA ACCTGGCTGT AACATTCGAA GATTATGGTT GGGACAGTGT 
TGCCTGTCCC ATCAGTGCTG CTTCAACATC TCCTCTGTCC TGAGCAACAA CCAGAAGTTG 
GTGGAACTGG ATCTGAGCCA CAATGCCCTG GGAGACTTTG GGATCAGACT TTTGTGTGTG 
GGACTGAGGC ATCTATTCTG CAAGCTAAAG AAGCTCTGGT TGGTCAGTTG CTGTCTCACA 
TCAGCGTGTT GTGAGGATCT TGCGTCCGTC CTGAGCAGCA ATCATTCCCT GACCAGACTC 
TACTTGGGTG AAAATGCCCT GGGAGACTCA GGAGTTGGAA TTTTATGTGA AAAAGCAAAG 
CATCCACAAT GTAACCTGCA AAAACTGGGG TTGGTGAATT CTGGCCTTAC ATCAGGTTGT 
TGTCTAGCTC TATCCTCAGT GCTCAGCACG AACCAGAATC TCACTCACCT CTATCTACGG 
GGAAATGCTC TTGGAGACAT CGGAGTCAAG CAACTCTGTG AGGGACTGTT GCACCCGAAC 
TGCAAGCTTC AAGTGTTGGA ATTAGAGAAC TGCAGCCTCA CATCACACTG CTGCTGGGAT 
CTTTCCACAC TTCTGACTTC TAACCAGAGC CTGCGAAAGC TGAGCCTAGG CAACAATGAC 
CTGGGTGATC TGGGGGTCAT GCTACTCTGT GAAGTGTTGA AACAGCAGGG GTGCCTCCTG 
AAAAGCCTTA AGTTGTGTGA AATGTATTTC AATTATGATA CAAAACATGC ATTACAAACA 
CTGCAAGAAG AAAAGCCTGA GTTGACCATT GTCTTTGAGC CTTCCCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP3 ( NM_001256770.2 ) cDNA ORF clone, Sus scrofa(pig) -> NP_001243699.1 Sus scrofa NLR family pyrin domain containing 3 (NLRP3), mRNA.

Price & Availability↑

CloneID	OSe30559
Clone ID Related Accession (Same CDS sequence)	NM_001256770.1 , NM_001256770.2
Accession Version	NM_001256770.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3111bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-30
Organism	Sus scrofa(pig)
Product	NACHT, LRR and PYD domains-containing protein 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from JQ219660.1. On May 16, 2017 this sequence version replaced NM_001256770.1. ##Evidence-Data-START## Transcript exon combination :: JQ219660.1, SRR5250921.83247.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA103886111, SAMEA103886112 [ECO:0000350] ##Evidence-Data-END##

ATGAGCATGG CAAGCGTCCG CTGCAAGCTG GCTCGTTACC TGGAGGACCT AGAAGATGTA 
GATTTTAAGA AATTCAAGAT GCACTTAGAA GACTATCCTT CTCAGAAGGG CTGCATCTCT 
CTCCCTCGGG GCCAGACAGA AAAAGCAGAT CATGTGGATC TAGCCACTCT GATGATAGAT 
TTCAATGGAG AGGAGAAGGC ATGGGCCATG GCCACATGGA TTTTTGCTGC GATCAACAGG 
AGAGACCTTT ATGAGAAAGC TAAGAGGGAT GAGCCAGAAT GGGACAATGC AAATCTTTCT 
GTGATAAGCC AGGAAGAAAG CCTTGAAGAG GAATGGATGG GTTTACTGGG GTACCTTTCC 
AGAATCTCTA TTTGTAAGAA AAAGAAAGAT TATTGTAAGA AGTACAGAAA GCACGTGAGA 
AGCAGATTCC AGTGCATCAA AGACAGGAAT GCACGTCTGG GTGAGAGTGT GAACCTCAAC 
AAACGCTTCA CCAGGCTGCG TCTCATCAAG GAACACCGGA GTCAGCAGGA GAGGGAGCAT 
GAGCTCCTTG CCATTGGCAG GACCTCAGCC AAGATGCAAG ATGGCCCCGT GAGTTCCCTG 
AACTTGGAAT TGCTGTTTGA TCCTGAGGAC CAACACTCTG AGCCTGTGCA CACGGTAGTA 
TTCCAGGGAG CAGCAGGCAT TGGGAAAACA ATACTGGCCA GGAAGATCAT GTTGGACTGG 
GCATCAGAGA AACTTTACCA GGAAAAATTT GACTATTTGT TTTACATTCA CTGTCGGGAG 
GTGAGCCTAG GAACTCGGAG GAGCCTGGGA GACCTGATCG CCAGCTGCTG CCCTGGCCCA 
AACCCACCCA TAGGCAAGAT TGTGAGCAAG CCTTCCAGGA TCCTCTTCCT CATGGATGGC 
TTTGATGAGC TGCAAGGTGC CTTTGATGAG CGCACAGAGG CACTCTGCAC AAACTGGCAG 
AAGGTGGAGC GGGGAGACAT TCTCCTGAGC AGCCTCATCA GAAAAAGACT GCTTCCTGAG 
GCCTCCCTGC TCATCACCAC AAGACCTGTG GCCCTGGAGA AACTTCAGCA CTTGCTGGGC 
CGGGCTCGTC ATGTGGAGAT TCTGGGTTTC TCAGAGGCCA AGAGGAAGGA ATATTTCTTT 
AAGTATTTTT CAGATGAACA GCAAGCAAGG GAAGCCTTCA GGCTGATTCA GGAGAATGAG 
GTCCTGTTCA CCATGTGCTT TATTCCCCTG GTCTGCTGGA TTGTGTGCAC TGGGCTGAAA 
CAGCAGATGG ATAGCGGCAA GAGTCTTGCC AGGACATCCA AGACCACCAC TGCCGTGTAT 
ATCTTCTTCC TCTCCACTTT GTTGCAATCT CAAGAAGGGA GCCAGGACCA CCACGTCTCT 
GCCACCCTCT GGGGTCTCTG CTCACTGGCT GCGGATGGAA TCTGGAATCA GAAAATCCTG 
TTTGAGGAGT GTGATCTCAG GAACCATGGC CTGCAGAAGG CAGATGTGTC TGCTTTCCTG 
AGGATGAACC TATTCCAAAA GGAAGTAGAC TGTGAGAAAT TCTACAGCTT CATTCACATG 
ACTTTCCAGG AGTTCTTTGC TGCCATGTAC TATCTACTGG AAAAGGAGGA TCAGGGGGAG 
ATGAGGAATG TGCCACGAAG CTGTTTGAAG CTTCCCAGTC GAGACGTGAC AGTTCTTCTT 
GAAAACTATG GCAAATTTGA AAAGGGGTAT CTGATTTTTG TTGTCCGTTT CCTCTTTGGC 
CTTGTAAACC AGGAGAGAAC GTCCTACTTG GAGAAAAAAC TGAGTTGTAA GATCTCTCAG 
AAAATCAGGC TAGAGCTGCT TAAATGGATT GAAGCGAAAG CCAAGGCCAA GAAGCTACAG 
ATTGAGCCCA GCCAGCTGGA ATTATTTTAC TGTTTGTACG AAATGCAGGA GGAGGACTTT 
GTGCAAAAGG CCATGGGCCA TTTCCCTAAA ATTGAGATCA ATCTCTCCAC CAGAATGGAC 
CATGTAGTTT CTTCTTTTTG TATTGAGAAC TGTCGCCATG TGGAATCACT TTCTCTGAGG 
TTGCTCCATA ATTCACCCAA AGAGGAGGAG GAGGAAGAGG AGATAGAGGA GGAGGATGTT 
CAACACTCTG ATGTGGACAA TTGTATCCTC TCTGAGTCTC ATGTTGCATA TTCTCAGCGA 
TTGGTGAACT ATCTCACTTC CAGCTTTTGT AGTGGCATCT TCTCAGTCCT GAGCAATAAC 
TGGAATCTCA CTGAATTGAA CCTCAGTGGT AACAGCCTGG GAGACCCAGG GATGAAGGTG 
TTATGTGAAA CGCTCCAGCA ACCTGGCTGT AACATTCGAA GATTATGGTT GGGACAGTGT 
TGCCTGTCCC ATCAGTGCTG CTTCAACATC TCCTCTGTCC TGAGCAACAA CCAGAAGTTG 
GTGGAACTGG ATCTGAGCCA CAATGCCCTG GGAGACTTTG GGATCAGACT TTTGTGTGTG 
GGACTGAGGC ATCTATTCTG CAAGCTAAAG AAGCTCTGGT TGGTCAGTTG CTGTCTCACA 
TCAGCGTGTT GTGAGGATCT TGCGTCCGTC CTGAGCAGCA ATCATTCCCT GACCAGACTC 
TACTTGGGTG AAAATGCCCT GGGAGACTCA GGAGTTGGAA TTTTATGTGA AAAAGCAAAG 
CATCCACAAT GTAACCTGCA AAAACTGGGG TTGGTGAATT CTGGCCTTAC ATCAGGTTGT 
TGTCTAGCTC TATCCTCAGT GCTCAGCACG AACCAGAATC TCACTCACCT CTATCTACGG 
GGAAATGCTC TTGGAGACAT CGGAGTCAAG CAACTCTGTG AGGGACTGTT GCACCCGAAC 
TGCAAGCTTC AAGTGTTGGA ATTAGAGAAC TGCAGCCTCA CATCACACTG CTGCTGGGAT 
CTTTCCACAC TTCTGACTTC TAACCAGAGC CTGCGAAAGC TGAGCCTAGG CAACAATGAC 
CTGGGTGATC TGGGGGTCAT GCTACTCTGT GAAGTGTTGA AACAGCAGGG GTGCCTCCTG 
AAAAGCCTTA AGTTGTGTGA AATGTATTTC AATTATGATA CAAAACATGC ATTACAAACA 
CTGCAAGAAG AAAAGCCTGA GTTGACCATT GTCTTTGAGC CTTCCCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243699.1
CDS	132..3242
Translation	MSMASVRCKLARYLEDLEDVDFKKFKMHLEDYPSQKGCISLPRGQTEKADHVDLATLMIDFNGEEKAWAMATWIFAAINRRDLYEKAKRDEPEWDNANLSVISQEESLEEEWMGLLGYLSRISICKKKKDYCKKYRKHVRSRFQCIKDRNARLGESVNLNKRFTRLRLIKEHRSQQEREHELLAIGRTSAKMQDGPVSSLNLELLFDPEDQHSEPVHTVVFQGAAGIGKTILARKIMLDWASEKLYQEKFDYLFYIHCREVSLGTRRSLGDLIASCCPGPNPPIGKIVSKPSRILFLMDGFDELQGAFDERTEALCTNWQKVERGDILLSSLIRKRLLPEASLLITTRPVALEKLQHLLGRARHVEILGFSEAKRKEYFFKYFSDEQQAREAFRLIQENEVLFTMCFIPLVCWIVCTGLKQQMDSGKSLARTSKTTTAVYIFFLSTLLQSQEGSQDHHVSATLWGLCSLAADGIWNQKILFEECDLRNHGLQKADVSAFLRMNLFQKEVDCEKFYSFIHMTFQEFFAAMYYLLEKEDQGEMRNVPRSCLKLPSRDVTVLLENYGKFEKGYLIFVVRFLFGLVNQERTSYLEKKLSCKISQKIRLELLKWIEAKAKAKKLQIEPSQLELFYCLYEMQEEDFVQKAMGHFPKIEINLSTRMDHVVSSFCIENCRHVESLSLRLLHNSPKEEEEEEEIEEEDVQHSDVDNCILSESHVAYSQRLVNYLTSSFCSGIFSVLSNNWNLTELNLSGNSLGDPGMKVLCETLQQPGCNIRRLWLGQCCLSHQCCFNISSVLSNNQKLVELDLSHNALGDFGIRLLCVGLRHLFCKLKKLWLVSCCLTSACCEDLASVLSSNHSLTRLYLGENALGDSGVGILCEKAKHPQCNLQKLGLVNSGLTSGCCLALSSVLSTNQNLTHLYLRGNALGDIGVKQLCEGLLHPNCKLQVLELENCSLTSHCCWDLSTLLTSNQSLRKLSLGNNDLGDLGVMLLCEVLKQQGCLLKSLKLCEMYFNYDTKHALQTLQEEKPELTIVFEPSQ

Target ORF information:

RefSeq Version	NM_001256770.2
Organism	Sus scrofa(pig)
Definition	Sus scrofa NLR family pyrin domain containing 3 (NLRP3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256770.2

ORF Insert Sequence:

ATGAGCATGG CAAGCGTCCG CTGCAAGCTG GCTCGTTACC TGGAGGACCT AGAAGATGTA 
GATTTTAAGA AATTCAAGAT GCACTTAGAA GACTATCCTT CTCAGAAGGG CTGCATCTCT 
CTCCCTCGGG GCCAGACAGA AAAAGCAGAT CATGTGGATC TAGCCACTCT GATGATAGAT 
TTCAATGGAG AGGAGAAGGC ATGGGCCATG GCCACATGGA TTTTTGCTGC GATCAACAGG 
AGAGACCTTT ATGAGAAAGC TAAGAGGGAT GAGCCAGAAT GGGACAATGC AAATCTTTCT 
GTGATAAGCC AGGAAGAAAG CCTTGAAGAG GAATGGATGG GTTTACTGGG GTACCTTTCC 
AGAATCTCTA TTTGTAAGAA AAAGAAAGAT TATTGTAAGA AGTACAGAAA GCACGTGAGA 
AGCAGATTCC AGTGCATCAA AGACAGGAAT GCACGTCTGG GTGAGAGTGT GAACCTCAAC 
AAACGCTTCA CCAGGCTGCG TCTCATCAAG GAACACCGGA GTCAGCAGGA GAGGGAGCAT 
GAGCTCCTTG CCATTGGCAG GACCTCAGCC AAGATGCAAG ATGGCCCCGT GAGTTCCCTG 
AACTTGGAAT TGCTGTTTGA TCCTGAGGAC CAACACTCTG AGCCTGTGCA CACGGTAGTA 
TTCCAGGGAG CAGCAGGCAT TGGGAAAACA ATACTGGCCA GGAAGATCAT GTTGGACTGG 
GCATCAGAGA AACTTTACCA GGAAAAATTT GACTATTTGT TTTACATTCA CTGTCGGGAG 
GTGAGCCTAG GAACTCGGAG GAGCCTGGGA GACCTGATCG CCAGCTGCTG CCCTGGCCCA 
AACCCACCCA TAGGCAAGAT TGTGAGCAAG CCTTCCAGGA TCCTCTTCCT CATGGATGGC 
TTTGATGAGC TGCAAGGTGC CTTTGATGAG CGCACAGAGG CACTCTGCAC AAACTGGCAG 
AAGGTGGAGC GGGGAGACAT TCTCCTGAGC AGCCTCATCA GAAAAAGACT GCTTCCTGAG 
GCCTCCCTGC TCATCACCAC AAGACCTGTG GCCCTGGAGA AACTTCAGCA CTTGCTGGGC 
CGGGCTCGTC ATGTGGAGAT TCTGGGTTTC TCAGAGGCCA AGAGGAAGGA ATATTTCTTT 
AAGTATTTTT CAGATGAACA GCAAGCAAGG GAAGCCTTCA GGCTGATTCA GGAGAATGAG 
GTCCTGTTCA CCATGTGCTT TATTCCCCTG GTCTGCTGGA TTGTGTGCAC TGGGCTGAAA 
CAGCAGATGG ATAGCGGCAA GAGTCTTGCC AGGACATCCA AGACCACCAC TGCCGTGTAT 
ATCTTCTTCC TCTCCACTTT GTTGCAATCT CAAGAAGGGA GCCAGGACCA CCACGTCTCT 
GCCACCCTCT GGGGTCTCTG CTCACTGGCT GCGGATGGAA TCTGGAATCA GAAAATCCTG 
TTTGAGGAGT GTGATCTCAG GAACCATGGC CTGCAGAAGG CAGATGTGTC TGCTTTCCTG 
AGGATGAACC TATTCCAAAA GGAAGTAGAC TGTGAGAAAT TCTACAGCTT CATTCACATG 
ACTTTCCAGG AGTTCTTTGC TGCCATGTAC TATCTACTGG AAAAGGAGGA TCAGGGGGAG 
ATGAGGAATG TGCCACGAAG CTGTTTGAAG CTTCCCAGTC GAGACGTGAC AGTTCTTCTT 
GAAAACTATG GCAAATTTGA AAAGGGGTAT CTGATTTTTG TTGTCCGTTT CCTCTTTGGC 
CTTGTAAACC AGGAGAGAAC GTCCTACTTG GAGAAAAAAC TGAGTTGTAA GATCTCTCAG 
AAAATCAGGC TAGAGCTGCT TAAATGGATT GAAGCGAAAG CCAAGGCCAA GAAGCTACAG 
ATTGAGCCCA GCCAGCTGGA ATTATTTTAC TGTTTGTACG AAATGCAGGA GGAGGACTTT 
GTGCAAAAGG CCATGGGCCA TTTCCCTAAA ATTGAGATCA ATCTCTCCAC CAGAATGGAC 
CATGTAGTTT CTTCTTTTTG TATTGAGAAC TGTCGCCATG TGGAATCACT TTCTCTGAGG 
TTGCTCCATA ATTCACCCAA AGAGGAGGAG GAGGAAGAGG AGATAGAGGA GGAGGATGTT 
CAACACTCTG ATGTGGACAA TTGTATCCTC TCTGAGTCTC ATGTTGCATA TTCTCAGCGA 
TTGGTGAACT ATCTCACTTC CAGCTTTTGT AGTGGCATCT TCTCAGTCCT GAGCAATAAC 
TGGAATCTCA CTGAATTGAA CCTCAGTGGT AACAGCCTGG GAGACCCAGG GATGAAGGTG 
TTATGTGAAA CGCTCCAGCA ACCTGGCTGT AACATTCGAA GATTATGGTT GGGACAGTGT 
TGCCTGTCCC ATCAGTGCTG CTTCAACATC TCCTCTGTCC TGAGCAACAA CCAGAAGTTG 
GTGGAACTGG ATCTGAGCCA CAATGCCCTG GGAGACTTTG GGATCAGACT TTTGTGTGTG 
GGACTGAGGC ATCTATTCTG CAAGCTAAAG AAGCTCTGGT TGGTCAGTTG CTGTCTCACA 
TCAGCGTGTT GTGAGGATCT TGCGTCCGTC CTGAGCAGCA ATCATTCCCT GACCAGACTC 
TACTTGGGTG AAAATGCCCT GGGAGACTCA GGAGTTGGAA TTTTATGTGA AAAAGCAAAG 
CATCCACAAT GTAACCTGCA AAAACTGGGG TTGGTGAATT CTGGCCTTAC ATCAGGTTGT 
TGTCTAGCTC TATCCTCAGT GCTCAGCACG AACCAGAATC TCACTCACCT CTATCTACGG 
GGAAATGCTC TTGGAGACAT CGGAGTCAAG CAACTCTGTG AGGGACTGTT GCACCCGAAC 
TGCAAGCTTC AAGTGTTGGA ATTAGAGAAC TGCAGCCTCA CATCACACTG CTGCTGGGAT 
CTTTCCACAC TTCTGACTTC TAACCAGAGC CTGCGAAAGC TGAGCCTAGG CAACAATGAC 
CTGGGTGATC TGGGGGTCAT GCTACTCTGT GAAGTGTTGA AACAGCAGGG GTGCCTCCTG 
AAAAGCCTTA AGTTGTGTGA AATGTATTTC AATTATGATA CAAAACATGC ATTACAAACA 
CTGCAAGAAG AAAAGCCTGA GTTGACCATT GTCTTTGAGC CTTCCCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of the Q969R gain-of-function polymorphism in the gene encoding porcine NLRP3 and its distribution in pigs of Asian and European origin.
Immunogenetics68(9)693-701(2016 10)
Tohno M,Shinkai H,Toki D,Okumura N,Tajima K,Uenishi H

The anti-inflammatory effects of baicalin through suppression of NLRP3 inflammasome pathway in LPS-challenged piglet mononuclear phagocytes.
Innate immunity22(3)196-204(2016 Apr)
Ye C,Li S,Yao W,Xu L,Qiu Y,Liu Y,Wu Z,Hou Y

Activation of sterol regulatory element binding protein and NLRP3 inflammasome in atherosclerotic lesion development in diabetic pigs.
PloS one8(6)e67532(2013)
Li Y,Xu S,Jiang B,Cohen RA,Zang M

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NLRP3 cDNA ORF clone, Sus scrofa(pig)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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