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ROBO4 cDNA ORF clone, Sus scrofa(pig)

Summary

Gene Symbol ROBO4
Entrez Gene ID 100524821
Full Name roundabout guidance receptor 4
Gene Type protein-coding
Organism Sus scrofa(pig)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_013979531.1 XP_013834985.1 roundabout homolog 4
XM_013979531.2 XP_013834985.1 roundabout homolog 4

Pathways from BioSystems


Gene Ontology

The following ROBO4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ROBO4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSe39684 XM_013979531.2
Latest version!		 Sus scrofa roundabout guidance receptor 4 (ROBO4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
View Old Accession Versions >>
OSe39684 XM_013979531.1 Sus scrofa roundabout guidance receptor 4 (ROBO4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OSe39684
Clone ID Related Accession (Same CDS sequence) XM_013979531.1 , XM_013979531.2
Accession Version XM_013979531.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3033bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-09-10
Organism Sus scrofa(Pig)
Product roundabout homolog 4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003611049.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
ATGGGCTCTG GAGGAACCGG CCTGCTTGCG GCCCATTGGC CTCTGCCTCT CCTGCTTCTG 
CTCCTGACGG GAGGCCTGGC TCAGGACTCC CCACCCCAGA TCCTAGTCCA CCCTGAGGAC 
CAGCTGCTCC AGGGCCCAGG CCCAGCCAGG ATGAGCTGCC GGGCCTCAGG CCAGCCACCA 
CCCACCATCC GCTGGCTGCT GAATGGGCAG CCCCTGAGCA TGGTTCCCCC CGACATTCAC 
TACCTCTTAC CTGATGGAAC CCTCCTGCTG CTGCGGCCCC CTCCCCGGGG ACGTGCCCAC 
GATGACCAGG CCCTATCCAC AGACCTGGGC ATCTACGCGT GTGAGGCCAG CAACCGGCTG 
GGCACAGCAG TCAGCCGGGG CGCTCGGCTG TCTGTGGCTG TCCTCCGGGA GGATTTCCTG 
ATTCAGCCTC GGGATACGGT GGCCACGGCG GGTGAGCAGG TGATTCTGGA GTGTGGGCCT 
CCTTGGGGCC ACCCAGAGCC CACAGTCTCA TGGTGGAAAG ATGGGAAACC CCTGGTCCTG 
CAGCCAGGGC GGCACTCAGT GTCCAGAGGG TCCCTGCTGA TGGCAAGAGC AGAGAAGAGC 
GATGCAGGGA CCTACATGTG TGTGGCCACC AACAGCGCAG GAAAACGGGA AAGCCGGGCA 
GCCAGGGTGT CTGTCCAGGA GCCCCAGGAC TACAAGGAGC CCCTGGAGCT TCTGGCCGTG 
CGCATTCAGC TGGAAAACGT GACCCTGCTG AACCCGGACC CTGCAAAGGA CACCAAGCCT 
GGGCCTACTG TATGGCTCAG CTGGAAGGTG AGCGGCCCTG CTGCACCTGC TCAGTCCTAC 
ACAGCTCTGT TCAGGGCCCA GGCTGCTCCT GGAAGCCAGG GAGCTCCATG GACAGAGGCC 
CTGCTGGCTG GCTGGCAGAG TGCAGAGCTC GGGGGCCTCC ACTGGGGCCA AGACTATGAG 
TTCAAAGTGA GACCATCCTC CGGCCGGGCT CAAGGTCCCG ACAGCAACGT GCTGCTCCTG 
AGGCTACCTG AACAAGTGCC CAGCGCCCCT CCCCAGGAGG TGACTCTAAG ACCTGGCAAT 
GGCAGTGTCC TTGTGAGCTG GGTGCCACCA CCTGCCGAAA ACCACAATGG CATCATCCGT 
GGCTACCAGG TCTGGAGCCT GGGCAACACT TCACTGCCCC CAGCCAACTG GACCGTGGTG 
GGTGAGCAGA CCCAGCTGGA GATCGCCACC CACATGCCAG GTTCCTACTG CGTACAAGTG 
GCTGCAGTCA CTGGGGCTGG GGCTGGGCAG CCCAGCAGCC CTGTCTGCCT CCTGTTAGAG 
CAGGCCATGG AGCAAGCTGC CCAAGAACCC AGTAGGCATG GTCCATGGAC CCTGGAGCAG 
CTGAGGGCAG CTTTGAAGCA GCCAGAGGTC ATTGCCAGTG GGGGTGTTGT GCTCTGGCTG 
CTGCTGATGG GCATCGCCGT GTGCATCCAC CGCCGGCGCC GGGCTGGCGT GCACCTGGGC 
CCAGGTCTGT ACAGATATAC CAGTGAGGAT GCCATCCTAA AACACAGAAC GGATCACAGT 
GACTCCCCAT GGCTGGCAGA CACTTGGCGC TCCACCTCCG GCTCCCGGGA CATCAGTAGT 
AGCAGCAGCC TCAGCAGCCG GCTGGGAGTG GACCCCCGGG ACCCACTAGA CAGTCGTCGC 
TCCTTGATCT CCTGGGATCC CCGAAGCCCC GGTGTGCCCC TGCTCCCCGA CACCAGCACC 
TTTTATGGCT CCCTCATCGC TGAGCTGCCC TCCAGCCCCC CAGCCCGGCC AAGCCCCCAG 
GCCCCAGCTG TCAGGCGGCT CACCCCCCAG CTGACCCGGC TCTCCAGCCC CTGGCCCAGT 
TCAGACAGCC TCTGCGGCCG CAGGGGCCTC TCTTCTCCAC GCTTGTCTCT GGCCCCTGCA 
GAGGCTTGGA AGGCCAAAAA GAAGCAGGAG CTGCACCAGG CCAACAGCTC CCCGCTGCTC 
CAGGCCAGCC ACCCTTCGGA GCTCTGGGCC TGCGAATTGG GCAACAGAGG CTCCAAGAAC 
CTTTCCCAAA GCTCAGGCTA TGGCTCCCCA GGAGCTTCGC CTCGGGCTCT GGTTGCCTGG 
CGGGCCCTGG GACCACAGCT CCTCCGCTCC TCCAATGAGC TGGTGACTCG CCCTCTCCCC 
CCAGCACCCC TCTCCCCTCG TGGACCCCCC ACTCAGAGAC AACAGACCCA GCACTCGGTG 
GAACCCCCAG CTCCCTCCTG CCCTGTCCTG CCAGCAGCCC CGGTCCCAGT CCTTAGCCCC 
TCTGGTCCCC CCAGCCCCTC CAGCCCCCAG GCCTCCTCCC TCTCCGGTCC CAGCCCAGCC 
TCCAGTCGCC TGTCCAGCTC GTCACTGTCA TCGCTGGGGG AGGATCAGGA CAGTGTGCTG 
ACCCCTGAGG AGGTGGCCCT GTGCCTGGAG CTCAGTGAGG GTGAGGAGAC CCCCAGGAAC 
AGTGTTTCTC CTATGCCAAG AGCGCCCTCA CCCCCCATCA CCTACGGCTA CATCAGCGTC 
CCCACAGCCT CGGAACTGGC GGACATGGGC AGGGCTGGGG GAGGGGTGGG GTCCGAGGTG 
AGGGGCTTGT TGTGCCCACC TCGGCCCTGC CCCACGCCCA CCCCCAGCGA GGGTTCCTTG 
GCCAATGGCT GGGGCTCAGC TTCTGAGGAC AACGCCCCCA GCGCCAGAGC CAGCCTGGTC 
AGCTCCTCTG ACGGCTCCTT CCTTGCCGAC GCCCACTTCG CCCAGGCCCT GGCCATGGCT 
GTGGACAGCT TTGGCTTTGG TCTGGAGCCC AGGGAGGCCG ACTGTGTCTT CACAGATGCT 
TCGTCACCTC CCTCCCCCCG GGATGACCTC TTCCTGACCT CCACCTTCTC CCTGCCCTCG 
TGGGAGTGGA GGTCAGACTG GTTGGAGGAT GTGGAGAAAA ACCATGCCCA GCTGTTTGGC 
AGGGGGCTGC CTTCCCGGCC CTCCGACTCT CGGATCTCTT CCCAGAGAAG TCAGCTCAGC 
TGTCCTGCAC CCAAGGCTGG AGACTCCTCC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_013834985.1
CDS80..3112
Translation

Target ORF information:

RefSeq Version XM_013979531.1
Organism Sus scrofa(Pig)
Definition Sus scrofa roundabout guidance receptor 4 (ROBO4), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_013979531.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
ATGGGCTCTG GAGGAACCGG CCTGCTTGCG GCCCATTGGC CTCTGCCTCT CCTGCTTCTG 
CTCCTGACGG GAGGCCTGGC TCAGGACTCC CCACCCCAGA TCCTAGTCCA CCCTGAGGAC 
CAGCTGCTCC AGGGCCCAGG CCCAGCCAGG ATGAGCTGCC GGGCCTCAGG CCAGCCACCA 
CCCACCATCC GCTGGCTGCT GAATGGGCAG CCCCTGAGCA TGGTTCCCCC CGACATTCAC 
TACCTCTTAC CTGATGGAAC CCTCCTGCTG CTGCGGCCCC CTCCCCGGGG ACGTGCCCAC 
GATGACCAGG CCCTATCCAC AGACCTGGGC ATCTACGCGT GTGAGGCCAG CAACCGGCTG 
GGCACAGCAG TCAGCCGGGG CGCTCGGCTG TCTGTGGCTG TCCTCCGGGA GGATTTCCTG 
ATTCAGCCTC GGGATACGGT GGCCACGGCG GGTGAGCAGG TGATTCTGGA GTGTGGGCCT 
CCTTGGGGCC ACCCAGAGCC CACAGTCTCA TGGTGGAAAG ATGGGAAACC CCTGGTCCTG 
CAGCCAGGGC GGCACTCAGT GTCCAGAGGG TCCCTGCTGA TGGCAAGAGC AGAGAAGAGC 
GATGCAGGGA CCTACATGTG TGTGGCCACC AACAGCGCAG GAAAACGGGA AAGCCGGGCA 
GCCAGGGTGT CTGTCCAGGA GCCCCAGGAC TACAAGGAGC CCCTGGAGCT TCTGGCCGTG 
CGCATTCAGC TGGAAAACGT GACCCTGCTG AACCCGGACC CTGCAAAGGA CACCAAGCCT 
GGGCCTACTG TATGGCTCAG CTGGAAGGTG AGCGGCCCTG CTGCACCTGC TCAGTCCTAC 
ACAGCTCTGT TCAGGGCCCA GGCTGCTCCT GGAAGCCAGG GAGCTCCATG GACAGAGGCC 
CTGCTGGCTG GCTGGCAGAG TGCAGAGCTC GGGGGCCTCC ACTGGGGCCA AGACTATGAG 
TTCAAAGTGA GACCATCCTC CGGCCGGGCT CAAGGTCCCG ACAGCAACGT GCTGCTCCTG 
AGGCTACCTG AACAAGTGCC CAGCGCCCCT CCCCAGGAGG TGACTCTAAG ACCTGGCAAT 
GGCAGTGTCC TTGTGAGCTG GGTGCCACCA CCTGCCGAAA ACCACAATGG CATCATCCGT 
GGCTACCAGG TCTGGAGCCT GGGCAACACT TCACTGCCCC CAGCCAACTG GACCGTGGTG 
GGTGAGCAGA CCCAGCTGGA GATCGCCACC CACATGCCAG GTTCCTACTG CGTACAAGTG 
GCTGCAGTCA CTGGGGCTGG GGCTGGGCAG CCCAGCAGCC CTGTCTGCCT CCTGTTAGAG 
CAGGCCATGG AGCAAGCTGC CCAAGAACCC AGTAGGCATG GTCCATGGAC CCTGGAGCAG 
CTGAGGGCAG CTTTGAAGCA GCCAGAGGTC ATTGCCAGTG GGGGTGTTGT GCTCTGGCTG 
CTGCTGATGG GCATCGCCGT GTGCATCCAC CGCCGGCGCC GGGCTGGCGT GCACCTGGGC 
CCAGGTCTGT ACAGATATAC CAGTGAGGAT GCCATCCTAA AACACAGAAC GGATCACAGT 
GACTCCCCAT GGCTGGCAGA CACTTGGCGC TCCACCTCCG GCTCCCGGGA CATCAGTAGT 
AGCAGCAGCC TCAGCAGCCG GCTGGGAGTG GACCCCCGGG ACCCACTAGA CAGTCGTCGC 
TCCTTGATCT CCTGGGATCC CCGAAGCCCC GGTGTGCCCC TGCTCCCCGA CACCAGCACC 
TTTTATGGCT CCCTCATCGC TGAGCTGCCC TCCAGCCCCC CAGCCCGGCC AAGCCCCCAG 
GCCCCAGCTG TCAGGCGGCT CACCCCCCAG CTGACCCGGC TCTCCAGCCC CTGGCCCAGT 
TCAGACAGCC TCTGCGGCCG CAGGGGCCTC TCTTCTCCAC GCTTGTCTCT GGCCCCTGCA 
GAGGCTTGGA AGGCCAAAAA GAAGCAGGAG CTGCACCAGG CCAACAGCTC CCCGCTGCTC 
CAGGCCAGCC ACCCTTCGGA GCTCTGGGCC TGCGAATTGG GCAACAGAGG CTCCAAGAAC 
CTTTCCCAAA GCTCAGGCTA TGGCTCCCCA GGAGCTTCGC CTCGGGCTCT GGTTGCCTGG 
CGGGCCCTGG GACCACAGCT CCTCCGCTCC TCCAATGAGC TGGTGACTCG CCCTCTCCCC 
CCAGCACCCC TCTCCCCTCG TGGACCCCCC ACTCAGAGAC AACAGACCCA GCACTCGGTG 
GAACCCCCAG CTCCCTCCTG CCCTGTCCTG CCAGCAGCCC CGGTCCCAGT CCTTAGCCCC 
TCTGGTCCCC CCAGCCCCTC CAGCCCCCAG GCCTCCTCCC TCTCCGGTCC CAGCCCAGCC 
TCCAGTCGCC TGTCCAGCTC GTCACTGTCA TCGCTGGGGG AGGATCAGGA CAGTGTGCTG 
ACCCCTGAGG AGGTGGCCCT GTGCCTGGAG CTCAGTGAGG GTGAGGAGAC CCCCAGGAAC 
AGTGTTTCTC CTATGCCAAG AGCGCCCTCA CCCCCCATCA CCTACGGCTA CATCAGCGTC 
CCCACAGCCT CGGAACTGGC GGACATGGGC AGGGCTGGGG GAGGGGTGGG GTCCGAGGTG 
AGGGGCTTGT TGTGCCCACC TCGGCCCTGC CCCACGCCCA CCCCCAGCGA GGGTTCCTTG 
GCCAATGGCT GGGGCTCAGC TTCTGAGGAC AACGCCCCCA GCGCCAGAGC CAGCCTGGTC 
AGCTCCTCTG ACGGCTCCTT CCTTGCCGAC GCCCACTTCG CCCAGGCCCT GGCCATGGCT 
GTGGACAGCT TTGGCTTTGG TCTGGAGCCC AGGGAGGCCG ACTGTGTCTT CACAGATGCT 
TCGTCACCTC CCTCCCCCCG GGATGACCTC TTCCTGACCT CCACCTTCTC CCTGCCCTCG 
TGGGAGTGGA GGTCAGACTG GTTGGAGGAT GTGGAGAAAA ACCATGCCCA GCTGTTTGGC 
AGGGGGCTGC CTTCCCGGCC CTCCGACTCT CGGATCTCTT CCCAGAGAAG TCAGCTCAGC 
TGTCCTGCAC CCAAGGCTGG AGACTCCTCC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OSe39684
Clone ID Related Accession (Same CDS sequence) XM_013979531.1 , XM_013979531.2
Accession Version XM_013979531.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3033bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-05-12
Organism Sus scrofa(pig)
Product roundabout homolog 4
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010451.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_013979531.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
ATGGGCTCTG GAGGAACCGG CCTGCTTGCG GCCCATTGGC CTCTGCCTCT CCTGCTTCTG 
CTCCTGACGG GAGGCCTGGC TCAGGACTCC CCACCCCAGA TCCTAGTCCA CCCTGAGGAC 
CAGCTGCTCC AGGGCCCAGG CCCAGCCAGG ATGAGCTGCC GGGCCTCAGG CCAGCCACCA 
CCCACCATCC GCTGGCTGCT GAATGGGCAG CCCCTGAGCA TGGTTCCCCC CGACATTCAC 
TACCTCTTAC CTGATGGAAC CCTCCTGCTG CTGCGGCCCC CTCCCCGGGG ACGTGCCCAC 
GATGACCAGG CCCTATCCAC AGACCTGGGC ATCTACGCGT GTGAGGCCAG CAACCGGCTG 
GGCACAGCAG TCAGCCGGGG CGCTCGGCTG TCTGTGGCTG TCCTCCGGGA GGATTTCCTG 
ATTCAGCCTC GGGATACGGT GGCCACGGCG GGTGAGCAGG TGATTCTGGA GTGTGGGCCT 
CCTTGGGGCC ACCCAGAGCC CACAGTCTCA TGGTGGAAAG ATGGGAAACC CCTGGTCCTG 
CAGCCAGGGC GGCACTCAGT GTCCAGAGGG TCCCTGCTGA TGGCAAGAGC AGAGAAGAGC 
GATGCAGGGA CCTACATGTG TGTGGCCACC AACAGCGCAG GAAAACGGGA AAGCCGGGCA 
GCCAGGGTGT CTGTCCAGGA GCCCCAGGAC TACAAGGAGC CCCTGGAGCT TCTGGCCGTG 
CGCATTCAGC TGGAAAACGT GACCCTGCTG AACCCGGACC CTGCAAAGGA CACCAAGCCT 
GGGCCTACTG TATGGCTCAG CTGGAAGGTG AGCGGCCCTG CTGCACCTGC TCAGTCCTAC 
ACAGCTCTGT TCAGGGCCCA GGCTGCTCCT GGAAGCCAGG GAGCTCCATG GACAGAGGCC 
CTGCTGGCTG GCTGGCAGAG TGCAGAGCTC GGGGGCCTCC ACTGGGGCCA AGACTATGAG 
TTCAAAGTGA GACCATCCTC CGGCCGGGCT CAAGGTCCCG ACAGCAACGT GCTGCTCCTG 
AGGCTACCTG AACAAGTGCC CAGCGCCCCT CCCCAGGAGG TGACTCTAAG ACCTGGCAAT 
GGCAGTGTCC TTGTGAGCTG GGTGCCACCA CCTGCCGAAA ACCACAATGG CATCATCCGT 
GGCTACCAGG TCTGGAGCCT GGGCAACACT TCACTGCCCC CAGCCAACTG GACCGTGGTG 
GGTGAGCAGA CCCAGCTGGA GATCGCCACC CACATGCCAG GTTCCTACTG CGTACAAGTG 
GCTGCAGTCA CTGGGGCTGG GGCTGGGCAG CCCAGCAGCC CTGTCTGCCT CCTGTTAGAG 
CAGGCCATGG AGCAAGCTGC CCAAGAACCC AGTAGGCATG GTCCATGGAC CCTGGAGCAG 
CTGAGGGCAG CTTTGAAGCA GCCAGAGGTC ATTGCCAGTG GGGGTGTTGT GCTCTGGCTG 
CTGCTGATGG GCATCGCCGT GTGCATCCAC CGCCGGCGCC GGGCTGGCGT GCACCTGGGC 
CCAGGTCTGT ACAGATATAC CAGTGAGGAT GCCATCCTAA AACACAGAAC GGATCACAGT 
GACTCCCCAT GGCTGGCAGA CACTTGGCGC TCCACCTCCG GCTCCCGGGA CATCAGTAGT 
AGCAGCAGCC TCAGCAGCCG GCTGGGAGTG GACCCCCGGG ACCCACTAGA CAGTCGTCGC 
TCCTTGATCT CCTGGGATCC CCGAAGCCCC GGTGTGCCCC TGCTCCCCGA CACCAGCACC 
TTTTATGGCT CCCTCATCGC TGAGCTGCCC TCCAGCCCCC CAGCCCGGCC AAGCCCCCAG 
GCCCCAGCTG TCAGGCGGCT CACCCCCCAG CTGACCCGGC TCTCCAGCCC CTGGCCCAGT 
TCAGACAGCC TCTGCGGCCG CAGGGGCCTC TCTTCTCCAC GCTTGTCTCT GGCCCCTGCA 
GAGGCTTGGA AGGCCAAAAA GAAGCAGGAG CTGCACCAGG CCAACAGCTC CCCGCTGCTC 
CAGGCCAGCC ACCCTTCGGA GCTCTGGGCC TGCGAATTGG GCAACAGAGG CTCCAAGAAC 
CTTTCCCAAA GCTCAGGCTA TGGCTCCCCA GGAGCTTCGC CTCGGGCTCT GGTTGCCTGG 
CGGGCCCTGG GACCACAGCT CCTCCGCTCC TCCAATGAGC TGGTGACTCG CCCTCTCCCC 
CCAGCACCCC TCTCCCCTCG TGGACCCCCC ACTCAGAGAC AACAGACCCA GCACTCGGTG 
GAACCCCCAG CTCCCTCCTG CCCTGTCCTG CCAGCAGCCC CGGTCCCAGT CCTTAGCCCC 
TCTGGTCCCC CCAGCCCCTC CAGCCCCCAG GCCTCCTCCC TCTCCGGTCC CAGCCCAGCC 
TCCAGTCGCC TGTCCAGCTC GTCACTGTCA TCGCTGGGGG AGGATCAGGA CAGTGTGCTG 
ACCCCTGAGG AGGTGGCCCT GTGCCTGGAG CTCAGTGAGG GTGAGGAGAC CCCCAGGAAC 
AGTGTTTCTC CTATGCCAAG AGCGCCCTCA CCCCCCATCA CCTACGGCTA CATCAGCGTC 
CCCACAGCCT CGGAACTGGC GGACATGGGC AGGGCTGGGG GAGGGGTGGG GTCCGAGGTG 
AGGGGCTTGT TGTGCCCACC TCGGCCCTGC CCCACGCCCA CCCCCAGCGA GGGTTCCTTG 
GCCAATGGCT GGGGCTCAGC TTCTGAGGAC AACGCCCCCA GCGCCAGAGC CAGCCTGGTC 
AGCTCCTCTG ACGGCTCCTT CCTTGCCGAC GCCCACTTCG CCCAGGCCCT GGCCATGGCT 
GTGGACAGCT TTGGCTTTGG TCTGGAGCCC AGGGAGGCCG ACTGTGTCTT CACAGATGCT 
TCGTCACCTC CCTCCCCCCG GGATGACCTC TTCCTGACCT CCACCTTCTC CCTGCCCTCG 
TGGGAGTGGA GGTCAGACTG GTTGGAGGAT GTGGAGAAAA ACCATGCCCA GCTGTTTGGC 
AGGGGGCTGC CTTCCCGGCC CTCCGACTCT CGGATCTCTT CCCAGAGAAG TCAGCTCAGC 
TGTCCTGCAC CCAAGGCTGG AGACTCCTCC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_013834985.1
CDS92..3124
Translation

Target ORF information:

RefSeq Version XM_013979531.2
Organism Sus scrofa(pig)
Definition Sus scrofa roundabout guidance receptor 4 (ROBO4), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_013979531.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
ATGGGCTCTG GAGGAACCGG CCTGCTTGCG GCCCATTGGC CTCTGCCTCT CCTGCTTCTG 
CTCCTGACGG GAGGCCTGGC TCAGGACTCC CCACCCCAGA TCCTAGTCCA CCCTGAGGAC 
CAGCTGCTCC AGGGCCCAGG CCCAGCCAGG ATGAGCTGCC GGGCCTCAGG CCAGCCACCA 
CCCACCATCC GCTGGCTGCT GAATGGGCAG CCCCTGAGCA TGGTTCCCCC CGACATTCAC 
TACCTCTTAC CTGATGGAAC CCTCCTGCTG CTGCGGCCCC CTCCCCGGGG ACGTGCCCAC 
GATGACCAGG CCCTATCCAC AGACCTGGGC ATCTACGCGT GTGAGGCCAG CAACCGGCTG 
GGCACAGCAG TCAGCCGGGG CGCTCGGCTG TCTGTGGCTG TCCTCCGGGA GGATTTCCTG 
ATTCAGCCTC GGGATACGGT GGCCACGGCG GGTGAGCAGG TGATTCTGGA GTGTGGGCCT 
CCTTGGGGCC ACCCAGAGCC CACAGTCTCA TGGTGGAAAG ATGGGAAACC CCTGGTCCTG 
CAGCCAGGGC GGCACTCAGT GTCCAGAGGG TCCCTGCTGA TGGCAAGAGC AGAGAAGAGC 
GATGCAGGGA CCTACATGTG TGTGGCCACC AACAGCGCAG GAAAACGGGA AAGCCGGGCA 
GCCAGGGTGT CTGTCCAGGA GCCCCAGGAC TACAAGGAGC CCCTGGAGCT TCTGGCCGTG 
CGCATTCAGC TGGAAAACGT GACCCTGCTG AACCCGGACC CTGCAAAGGA CACCAAGCCT 
GGGCCTACTG TATGGCTCAG CTGGAAGGTG AGCGGCCCTG CTGCACCTGC TCAGTCCTAC 
ACAGCTCTGT TCAGGGCCCA GGCTGCTCCT GGAAGCCAGG GAGCTCCATG GACAGAGGCC 
CTGCTGGCTG GCTGGCAGAG TGCAGAGCTC GGGGGCCTCC ACTGGGGCCA AGACTATGAG 
TTCAAAGTGA GACCATCCTC CGGCCGGGCT CAAGGTCCCG ACAGCAACGT GCTGCTCCTG 
AGGCTACCTG AACAAGTGCC CAGCGCCCCT CCCCAGGAGG TGACTCTAAG ACCTGGCAAT 
GGCAGTGTCC TTGTGAGCTG GGTGCCACCA CCTGCCGAAA ACCACAATGG CATCATCCGT 
GGCTACCAGG TCTGGAGCCT GGGCAACACT TCACTGCCCC CAGCCAACTG GACCGTGGTG 
GGTGAGCAGA CCCAGCTGGA GATCGCCACC CACATGCCAG GTTCCTACTG CGTACAAGTG 
GCTGCAGTCA CTGGGGCTGG GGCTGGGCAG CCCAGCAGCC CTGTCTGCCT CCTGTTAGAG 
CAGGCCATGG AGCAAGCTGC CCAAGAACCC AGTAGGCATG GTCCATGGAC CCTGGAGCAG 
CTGAGGGCAG CTTTGAAGCA GCCAGAGGTC ATTGCCAGTG GGGGTGTTGT GCTCTGGCTG 
CTGCTGATGG GCATCGCCGT GTGCATCCAC CGCCGGCGCC GGGCTGGCGT GCACCTGGGC 
CCAGGTCTGT ACAGATATAC CAGTGAGGAT GCCATCCTAA AACACAGAAC GGATCACAGT 
GACTCCCCAT GGCTGGCAGA CACTTGGCGC TCCACCTCCG GCTCCCGGGA CATCAGTAGT 
AGCAGCAGCC TCAGCAGCCG GCTGGGAGTG GACCCCCGGG ACCCACTAGA CAGTCGTCGC 
TCCTTGATCT CCTGGGATCC CCGAAGCCCC GGTGTGCCCC TGCTCCCCGA CACCAGCACC 
TTTTATGGCT CCCTCATCGC TGAGCTGCCC TCCAGCCCCC CAGCCCGGCC AAGCCCCCAG 
GCCCCAGCTG TCAGGCGGCT CACCCCCCAG CTGACCCGGC TCTCCAGCCC CTGGCCCAGT 
TCAGACAGCC TCTGCGGCCG CAGGGGCCTC TCTTCTCCAC GCTTGTCTCT GGCCCCTGCA 
GAGGCTTGGA AGGCCAAAAA GAAGCAGGAG CTGCACCAGG CCAACAGCTC CCCGCTGCTC 
CAGGCCAGCC ACCCTTCGGA GCTCTGGGCC TGCGAATTGG GCAACAGAGG CTCCAAGAAC 
CTTTCCCAAA GCTCAGGCTA TGGCTCCCCA GGAGCTTCGC CTCGGGCTCT GGTTGCCTGG 
CGGGCCCTGG GACCACAGCT CCTCCGCTCC TCCAATGAGC TGGTGACTCG CCCTCTCCCC 
CCAGCACCCC TCTCCCCTCG TGGACCCCCC ACTCAGAGAC AACAGACCCA GCACTCGGTG 
GAACCCCCAG CTCCCTCCTG CCCTGTCCTG CCAGCAGCCC CGGTCCCAGT CCTTAGCCCC 
TCTGGTCCCC CCAGCCCCTC CAGCCCCCAG GCCTCCTCCC TCTCCGGTCC CAGCCCAGCC 
TCCAGTCGCC TGTCCAGCTC GTCACTGTCA TCGCTGGGGG AGGATCAGGA CAGTGTGCTG 
ACCCCTGAGG AGGTGGCCCT GTGCCTGGAG CTCAGTGAGG GTGAGGAGAC CCCCAGGAAC 
AGTGTTTCTC CTATGCCAAG AGCGCCCTCA CCCCCCATCA CCTACGGCTA CATCAGCGTC 
CCCACAGCCT CGGAACTGGC GGACATGGGC AGGGCTGGGG GAGGGGTGGG GTCCGAGGTG 
AGGGGCTTGT TGTGCCCACC TCGGCCCTGC CCCACGCCCA CCCCCAGCGA GGGTTCCTTG 
GCCAATGGCT GGGGCTCAGC TTCTGAGGAC AACGCCCCCA GCGCCAGAGC CAGCCTGGTC 
AGCTCCTCTG ACGGCTCCTT CCTTGCCGAC GCCCACTTCG CCCAGGCCCT GGCCATGGCT 
GTGGACAGCT TTGGCTTTGG TCTGGAGCCC AGGGAGGCCG ACTGTGTCTT CACAGATGCT 
TCGTCACCTC CCTCCCCCCG GGATGACCTC TTCCTGACCT CCACCTTCTC CCTGCCCTCG 
TGGGAGTGGA GGTCAGACTG GTTGGAGGAT GTGGAGAAAA ACCATGCCCA GCTGTTTGGC 
AGGGGGCTGC CTTCCCGGCC CTCCGACTCT CGGATCTCTT CCCAGAGAAG TCAGCTCAGC 
TGTCCTGCAC CCAAGGCTGG AGACTCCTCC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed