ZGRF1 cDNA ORF clone, Sus scrofa(pig)

The following ZGRF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ZGRF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSe39090 XM_005656543.2
Latest version!
Sus scrofa zinc finger, GRF-type containing 1 (ZGRF1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OSe224828 XM_021101665.1
Latest version!
Sus scrofa zinc finger GRF-type containing 1 (ZGRF1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OSe224829 XM_021101666.1
Latest version!
Sus scrofa zinc finger GRF-type containing 1 (ZGRF1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OSe224830 XM_021101667.1
Latest version!
Sus scrofa zinc finger GRF-type containing 1 (ZGRF1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OSe224831 XM_021101668.1
Latest version!
Sus scrofa zinc finger GRF-type containing 1 (ZGRF1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OSe224826 XM_021101663.1
Latest version!
Sus scrofa zinc finger GRF-type containing 1 (ZGRF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OSe224826 XM_021101661.1
Latest version!
Sus scrofa zinc finger GRF-type containing 1 (ZGRF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OSe224826 XM_021101662.1
Latest version!
Sus scrofa zinc finger GRF-type containing 1 (ZGRF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OSe224827 XM_021101664.1
Latest version!
Sus scrofa zinc finger GRF-type containing 1 (ZGRF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSe39090
    Clone ID Related Accession (Same CDS sequence) XM_005656543.2
    Accession Version XM_005656543.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2742bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-10
    Organism Sus scrofa(Pig)
    Product protein ZGRF1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003535436.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 11, 2015 this sequence version replaced XM_005656543.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCAA CAAAGGGCCC CTGTTGGGAA ACTGTAGAAT ATACAGCTAA AGAGGCCATT 
    TTATATGATG ACAAAGGACA GTGTTTGGAG AGTTTATTTC TTAAGTGCCT TGAGGTGAAA
    CCTGGAGATG ATTTAGAAAG TGATCGATAC TTAATCACAG TTGAGGAGGT TAAAGTTGCT
    GGAAGCATAG CTGTTAAACA AGATATCATT AAAGGAGCAC CTCCATTAAA TTCAAAGAGC
    TTTATACCTT CTGGCCGGTC CCTTGGGTGT CAACCTGCTG GTTTAAAAAG GAAATTTACT
    GGTTTTCAAG GACCACGGCA AGTGCCAAAG AAAATGGCTA TTACAGAAAA TAGTGAATCA
    GCTGCTTCAC TTGAGGCTAA GGAAGCTGGT CCTGTTTTTC TTTCTCCAGT CTACAGCACA
    CCTCCTTTGT TTCCTACTGT TGGCAAAAAA GATATAAATT ATATGCCAAC AGACCCTGAC
    AACCAGGACA GAGAGAGAAA TGGCATAGCT TTTTCTTCGG TTGTCTCTAC TCCATCCTTC
    AAGATTAACC CAGAAATGCT ATGTGAAGAA AATTATTTTT GCTCTACTGT CAATTCTGTA
    AATAAGCATT CAGGTTCTTT ACTGACCGAT GAGCTTATGA AAAGAGATTG TTTGGTATCT
    CACAATTCAG GAGTTTCACA GAACATTAGA AGTAAAGCCC AGATATTGGC TCTTCTGAAA
    TCCAAATCCA CTGGTACATC TAAAGAACCA AATTCTGAGA TTACAGGACA TTTCCCTCTG
    ATACAACCAC AAGGAAGTTT AAAAATTCCT GCTAAACTTG AGTGCTTAAT TGAACAGGAA
    GAACACACTC AGGTGAAGAG CACAGAAAGT TTATACTGCC AACATCAATC AAAAAATACC
    ATGAGAAATA AAAGCCGGTG GGCCATGTAT TTATCCTCAC AGAGTTCACC TACACATTCT
    TCTACAGTAG ATGGAAATAA TACAGGAAAA AATCCTGAGG CCGAGGGAGG TAATATAAAT
    TTAAATTTGA AAGACGCTTT GGTGCAAAAA AGCATGCGGT TTTTAGAAAC ATGTACTGAA
    GAGGGAAAAA AGTATAATGA AGACAAGCCA GTAGTTGATA ATGATCAATC CTGGAATCAA
    AAAGTAAAAT TAGAATTTCC TTCATTCTGT GAAAGCAGTG CCTTACCAGT TACTTGTGAC
    AACGTAGAAA ACGACAGCTT ATTATCAGAA AATGACAGCT TATTATCAGA AAATGACATT
    CAAGAAAATA ATAAAAGACT TTTTAATCAA AATGACCAGA TGGGCATAAA AGGATCAATT
    CTCATTACAG AAAAAGCTCA GGAGATAACC ATGTATGGAA CCCCAGCTAA GGAGTATAAA
    CATCTGCAGA TTGAATCTTC ACTAAGTAAC GATTTTAGGG TCTCTAATGG CATTGCTGAC
    ATGTTTTCTA AAAGTAATGC TGATAATGAA AATCTGAACA GTATTCATGA ACCACTTGTG
    GAGATTACTT TTAATCTGAA CAATTTTGAA ACCAGTGACT CTGAGGAGGA ATCACAGGAA
    AGCAGCAGAA TTTCCCACGA TTCAGAGGGT TGGGTGAAGG AAACTTTAGT TAAAGACAAT
    TCAAGCTGTG AGAATATAAA CTGTGCAGAA GTTGGCAGTG ACCATTTGCC TCTCTTAACG
    TCTAGTGGGG ACAGACTTGC AGAGACATTT TCCATGAAAG AGACTCTGCT ATCACAATTT
    TGTGATAAAA CTTCTGTAGG TTTTGACACA GGACCTTGGA AAGCTGGAAA CACTAGAAAA
    GAAATAGAGG AGGAGTACGG TGACACCTCA AACAATCTTG ACTCATCTTT AGAGTGGACT
    GAAGATGCAA ATGTAGGTAG TAATGAAGGC ACTAATAAAT CTACTCAGGA AGTCACAGTT
    AACTATGATT TTGCTTCTCT CCCAAATGAA AGTATAAACA CAAATTTACA TATCCCTCAC
    TCTTTAAACA CAGCCACTAA TCAGGATCCT GAAAACAATC CATGGTCACA ACAGGCTCAG
    CCTCAGCCTT TTATTATGGG AACTGACTTA CACAAAAATA CTGAACAGGT TTTACCATTA
    ACTTCTAGCA GTGGCAACGG CACCCAACTA TTAAATGCCA GTCAGAATCA CTCTGAAGAA
    TTTCTTGCAC TTGATAAATC ACATACCCAA GTTTCCAATT CTTCGTTTTA CCCTCTGGGA
    GTAAAATATC CTATATCTAA AGACACAGAA TCACATATTC TGGAATCTGA AGATTTGGGA
    AGGACTAGAA GTTTACCTCA TGACAATACT GACATAGAAA CTGCCAGAGA GAGCAAACAG
    TACTGGAATA CTCCTAAAAA TTCTTCAGAA CTTTCTGGAT TAGAAAATAG CATTTCCCTA
    TTAAAATCAT TGTCTGAACA CAGTACTGCT TTAGAAGGCT TGGAAATATT GAAAAAGAAA
    CATACCTCCT TTAAGCAACA AGGAACTCTG CAGACGTATG ATCCAGAAAG CAGTCCTGAA
    GCTAGGAAAC CATTGACTAC AATAGTTTCA CAAGCTATAC CAAAGTCTCA TCCGAACCAG
    GATCCTCAAC AGATGATAAA AGAAAATGAA GTTAAACCAA GTGAATCCCT CACCAAGTTT
    TTTCCCTTGG GCAGTGAAGA AGAGACTGAC TCCCGAGCTC TCATTCCTAA ACAAATGGAG
    AGAAAAACCT GTGACCCTAA GGTAAAATTT GCGCAGAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005656600.2
    CDS1..2742
    Translation

    Target ORF information:

    RefSeq Version XM_005656543.2
    Organism Sus scrofa(Pig)
    Definition Sus scrofa zinc finger, GRF-type containing 1 (ZGRF1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005656543.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCAA CAAAGGGCCC CTGTTGGGAA ACTGTAGAAT ATACAGCTAA AGAGGCCATT 
    TTATATGATG ACAAAGGACA GTGTTTGGAG AGTTTATTTC TTAAGTGCCT TGAGGTGAAA
    CCTGGAGATG ATTTAGAAAG TGATCGATAC TTAATCACAG TTGAGGAGGT TAAAGTTGCT
    GGAAGCATAG CTGTTAAACA AGATATCATT AAAGGAGCAC CTCCATTAAA TTCAAAGAGC
    TTTATACCTT CTGGCCGGTC CCTTGGGTGT CAACCTGCTG GTTTAAAAAG GAAATTTACT
    GGTTTTCAAG GACCACGGCA AGTGCCAAAG AAAATGGCTA TTACAGAAAA TAGTGAATCA
    GCTGCTTCAC TTGAGGCTAA GGAAGCTGGT CCTGTTTTTC TTTCTCCAGT CTACAGCACA
    CCTCCTTTGT TTCCTACTGT TGGCAAAAAA GATATAAATT ATATGCCAAC AGACCCTGAC
    AACCAGGACA GAGAGAGAAA TGGCATAGCT TTTTCTTCGG TTGTCTCTAC TCCATCCTTC
    AAGATTAACC CAGAAATGCT ATGTGAAGAA AATTATTTTT GCTCTACTGT CAATTCTGTA
    AATAAGCATT CAGGTTCTTT ACTGACCGAT GAGCTTATGA AAAGAGATTG TTTGGTATCT
    CACAATTCAG GAGTTTCACA GAACATTAGA AGTAAAGCCC AGATATTGGC TCTTCTGAAA
    TCCAAATCCA CTGGTACATC TAAAGAACCA AATTCTGAGA TTACAGGACA TTTCCCTCTG
    ATACAACCAC AAGGAAGTTT AAAAATTCCT GCTAAACTTG AGTGCTTAAT TGAACAGGAA
    GAACACACTC AGGTGAAGAG CACAGAAAGT TTATACTGCC AACATCAATC AAAAAATACC
    ATGAGAAATA AAAGCCGGTG GGCCATGTAT TTATCCTCAC AGAGTTCACC TACACATTCT
    TCTACAGTAG ATGGAAATAA TACAGGAAAA AATCCTGAGG CCGAGGGAGG TAATATAAAT
    TTAAATTTGA AAGACGCTTT GGTGCAAAAA AGCATGCGGT TTTTAGAAAC ATGTACTGAA
    GAGGGAAAAA AGTATAATGA AGACAAGCCA GTAGTTGATA ATGATCAATC CTGGAATCAA
    AAAGTAAAAT TAGAATTTCC TTCATTCTGT GAAAGCAGTG CCTTACCAGT TACTTGTGAC
    AACGTAGAAA ACGACAGCTT ATTATCAGAA AATGACAGCT TATTATCAGA AAATGACATT
    CAAGAAAATA ATAAAAGACT TTTTAATCAA AATGACCAGA TGGGCATAAA AGGATCAATT
    CTCATTACAG AAAAAGCTCA GGAGATAACC ATGTATGGAA CCCCAGCTAA GGAGTATAAA
    CATCTGCAGA TTGAATCTTC ACTAAGTAAC GATTTTAGGG TCTCTAATGG CATTGCTGAC
    ATGTTTTCTA AAAGTAATGC TGATAATGAA AATCTGAACA GTATTCATGA ACCACTTGTG
    GAGATTACTT TTAATCTGAA CAATTTTGAA ACCAGTGACT CTGAGGAGGA ATCACAGGAA
    AGCAGCAGAA TTTCCCACGA TTCAGAGGGT TGGGTGAAGG AAACTTTAGT TAAAGACAAT
    TCAAGCTGTG AGAATATAAA CTGTGCAGAA GTTGGCAGTG ACCATTTGCC TCTCTTAACG
    TCTAGTGGGG ACAGACTTGC AGAGACATTT TCCATGAAAG AGACTCTGCT ATCACAATTT
    TGTGATAAAA CTTCTGTAGG TTTTGACACA GGACCTTGGA AAGCTGGAAA CACTAGAAAA
    GAAATAGAGG AGGAGTACGG TGACACCTCA AACAATCTTG ACTCATCTTT AGAGTGGACT
    GAAGATGCAA ATGTAGGTAG TAATGAAGGC ACTAATAAAT CTACTCAGGA AGTCACAGTT
    AACTATGATT TTGCTTCTCT CCCAAATGAA AGTATAAACA CAAATTTACA TATCCCTCAC
    TCTTTAAACA CAGCCACTAA TCAGGATCCT GAAAACAATC CATGGTCACA ACAGGCTCAG
    CCTCAGCCTT TTATTATGGG AACTGACTTA CACAAAAATA CTGAACAGGT TTTACCATTA
    ACTTCTAGCA GTGGCAACGG CACCCAACTA TTAAATGCCA GTCAGAATCA CTCTGAAGAA
    TTTCTTGCAC TTGATAAATC ACATACCCAA GTTTCCAATT CTTCGTTTTA CCCTCTGGGA
    GTAAAATATC CTATATCTAA AGACACAGAA TCACATATTC TGGAATCTGA AGATTTGGGA
    AGGACTAGAA GTTTACCTCA TGACAATACT GACATAGAAA CTGCCAGAGA GAGCAAACAG
    TACTGGAATA CTCCTAAAAA TTCTTCAGAA CTTTCTGGAT TAGAAAATAG CATTTCCCTA
    TTAAAATCAT TGTCTGAACA CAGTACTGCT TTAGAAGGCT TGGAAATATT GAAAAAGAAA
    CATACCTCCT TTAAGCAACA AGGAACTCTG CAGACGTATG ATCCAGAAAG CAGTCCTGAA
    GCTAGGAAAC CATTGACTAC AATAGTTTCA CAAGCTATAC CAAAGTCTCA TCCGAACCAG
    GATCCTCAAC AGATGATAAA AGAAAATGAA GTTAAACCAA GTGAATCCCT CACCAAGTTT
    TTTCCCTTGG GCAGTGAAGA AGAGACTGAC TCCCGAGCTC TCATTCCTAA ACAAATGGAG
    AGAAAAACCT GTGACCCTAA GGTAAAATTT GCGCAGAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe224828
    Clone ID Related Accession (Same CDS sequence) XM_021101665.1
    Accession Version XM_021101665.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5655bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product protein ZGRF1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010450.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGAAAGCC AGGAATTTAT TGTCCTATAT ACTCATCAAA AGATGAAGAA GTCAAAAGTA 
    TGGCAAGATG GAATTCTGAA GATCACTCAC GTAGGAAACA AAGCCATTTT ATATGATGAC
    AAAGGACAGT GTTTGGAGAG TTTATTTCTT AAGTGCCTTG AGGTGAAACC TGGAGATGAT
    TTAGAAAGTG ATCGATACTT AATCACAGTT GAGGAGGTTA AAGTTGCTGG AAGCATAGCT
    GTTAAACAAG ATATCATTAA AGGAGCACCT CCATTAAATT CAAAGAGCTT TATACCTTCT
    GGCCGGTCCC TTGGGTGTCA ACCTGCTGGT TTAAAAAGGA AATTTACTGG TTTTCAAGGA
    CCACGGCAAG TGCCAAAGAA AATGGCTATT ACAGAAAATA GTGAATCAGC TGCTTCACTT
    GAGGCTAAGG AAGCTGGTCC TGTTTTTCTT TCTCCAGTCT ACAGCACACC TCCTTTGTTT
    CCTACTGTTG GCAAAAAAGA TATAAATTAT ATGCCAACAG ACCCTGACAA CCAGGACAGA
    GAGAGAAATG GCATAGCTTT TTCTTCGGTT GTCTCTACTC CATCCTTCAA GATTAACCCA
    GAAATGCTAT GTGAAGAAAA TTATTTTTGC TCTACTGTCA ATTCTGTAAA TAAGCATTCA
    GGTTCTTTAC TGACCGATGA GCTTATGAAA AGAGATTGTT TGGTATCTCA CAATTCAGGA
    GTTTCACAGA ACATTAGAAG TAAAGCCCAG ATATTGGCTC TTCTGAAATC CAAATCCACT
    GGTACATCTA AAGAACCAAA TTCTGAGATT ACAGGACATT TCCCTCTGAT ACAACCACAA
    GGAAGTTTAA AAATTCCTGC TAAACTTGAG TGCTTAATTG AACAGGAAGA ACACACTCAG
    GTGAAGAGCA CAGAAAGTTT ATACTGCCAA CATCAATCAA AAAATACCAT GAGAAATAAA
    AGCCGGTGGG CCATGTATTT ATCCTCACAG AGTTCACCTA CACATTCTTC TACAGTAGAT
    GGAAATAATA CAGGAAAAAA TCCTGAGGCC GAGGGAGGTA ATATAAATTT AAATTTGAAA
    GACGCTTTGG TGCAAAAAAG CATGCGGTTT TTAGAAACAT GTACTGAAGA GGGAAAAAAG
    TATAATGAAG ACAAGCCAGT AGTTGATAAT GATCAATCCT GGAATCAAAA AGTAAAATTA
    GAATTTCCTT CATTCTGTGA AAGCAGTGCC TTACCAGTTA CTTGTGACAA CGTAGAAAAC
    GACAGCTTAT TATCAGAAAA TGACAGCTTA TTATCAGAAA ATGACATTCA AGAAAATAAT
    AAAAGACTTT TTAATCAAAA TGACCAGATG GGCATAAAAG GATCAATTCT CATTACAGAA
    AAAGCTCAGG AGATAACCAT GTATGGAACC CCAGCTAAGG AGTATAAACA TCTGCAGATT
    GAATCTTCAC TAAGTAACGA TTTTAGGGTC TCTAATGGCA TTGCTGACAT GTTTTCTAAA
    AGTAATGCTG ATAATGAAAA TCTGAACAGT ATTCATGAAC CACTTGTGGA GATTACTTTT
    AATCTGAACA ATTTTGAAAC CAGTGACTCT GAGGAGGAAT CACAGGAAAG CAGCAGAATT
    TCCCACGATT CAGAGGGTTG GGTGAAGGAA ACTTTAGTTA AAGACAATTC AAGCTGTGAG
    AATATAAACT GTGCAGAAGT TGGCAGTGAC CATTTGCCTC TCTTAACGTC TAGTGGGGAC
    AGACTTGCAG AGACATTTTC CATGAAAGAG ACTCTGCTAT CACAATTTTG TGATAAAACT
    TCTGTAGGTT TTGACACAGG ACCTTGGAAA GCTGGAAACA CTAGAAAAGA AATAGAGGAG
    GAGTACGGTG ACACCTCAAA CAATCTTGAC TCATCTTTAG AGTGGACTGA AGATGCAAAT
    GTAGGTAGTA ATGAAGGCAC TAATAAATCT ACTCAGGAAG TCACAGTTAA CTATGATTTT
    GCTTCTCTCC CAAATGAAAG TATAAACACA AATTTACATA TCCCTCACTC TTTAAACACA
    GCCACTAATC AGGATCCTGA AAACAATCCA TGGTCACAAC AGGCTCAGCC TCAGCCTTTT
    ATTATGGGAA CTGACTTACA CAAAAATACT GAACAGGTTT TACCATTAAC TTCTAGCAGT
    GGCAACGGCA CCCAACTATT AAATGCCAGT CAGAATCACT CTGAAGAATT TCTTGCACTT
    GATAAATCAC ATACCCAAGT TTCCAATTCT TCGTTTTACC CTCTGGGAGT AAAATATCCT
    ATATCTAAAG ACACAGAATC ACATATTCTG GAATCTGAAG ATTTGGGAAG GACTAGAAGT
    TTACCTCATG ACAATACTGA CATAGAAACT GCCAGAGAGA GCAAACAGTA CTGGAATACT
    CCTAAAAATT CTTCAGAACT TTCTGGATTA GAAAATAGCA TTTCCCTATT AAAATCATTG
    TCTGAACACA GTACTGCTTT AGAAGGCTTG GAAATATTGA AAAAGAAACA TACCTCCTTT
    AAGCAACAAG GAACTCTGCA GACGTATGAT CCAGAAAGCA GTCCTGAAGC TAGGAAACCA
    TTGACTACAA TAGTTTCACA AGCTATACCA AAGTCTCATC CGAACCAGGA TCCTCAACAG
    ATGATAAAAG AAAATGAAGT TAAACCAAGT GAATCCCTCA CCAAGTTTTT TCCCTTGGGC
    AGTGAAGAAG AGACTGACTC CCGAGCTCTC ATTCCTAAAC AAATGGAGAG AAAAACCTGT
    GACCCTAAGC CTGTTGAGTT TCAAGGGCAC CAAGTGAAAG GATCAGCTGC TAGTGCTGTG
    ATGGTCAGAG GACACAGCTC CCAGTTTGAA TGCAGTCAAT TTCCAGATAG CGTGTATGAA
    AACTGCATGA CAAATACTTG TTTTTTGACA CCTAAGTTGC CTAGCACTTG TATGCAGATT
    GACTTCTTGC AGTTGCCAGA TGAAGAAAAC TTGATAAAAG GAGATTCCAG CTTTAACACT
    GAAGACAATT TTCAAGTGAT AGCTGAGCCT AATAAGAATT CAGTTAAAAA TGATCTAATG
    AAAAAGTCTC TGGAGAATAA GAACCTTCAA AGGCTTTCAT TAGAGATAAG TGACTGTGAG
    CTGTGCTTCC CAAGTGGGGA GAAAGTAAAA TCTGCATATC TTCCCCAAAG GCAAATTCAC
    ATACCAGCTG TTTTTCAGTC TCCTGCTCAT TATAAACAGA TTTTTACATC TTGTCTCATA
    GAACATCTAA ATATATTGCT GTTTGGGTTG GCACAAAGGC TGCACAAAGC TCTTTCAAAA
    GTTGACATAT CATTTTACTC ATCAAACGGA GACAAACGGA AAAATGTAGA AAATAATGTG
    CCATCATGCC ATCATCATCA CCCTGCAAAA CTTGTCATGG TTAAAAAGGA AGGTCCAAAT
    AAGGGTCGTC TCTTTTATGC TTGTGATGGA CCCAAAGCTG ACCAATGTAA ATTTTTCAAG
    TGGCTTGATG AAGTGACCCC AGGATATTTA ACACAGGAAG AATCACTACC TAGCATGGTT
    TTAAGTGATA TAAAAAGTAT TGGCTTGTAC TTAAGAAGTC AAAAGATACC CCTCTATGAG
    GAATGCCAAC TTTTGGTGAG AAAAGGATTT GATTTTCAAA GAAAACGGTA TGGTAAACTA
    AAGAAGTTTA CAACTGTAGA TCCTGAATTT TATAACGAAC CTAAGAGCAG ACTTTATCTT
    AAGCTGAGTC GTAAGGAAAG TTCATCTGCT TATAGTAAAG ATGATCTTTG GGTGGTTTCA
    AAAACCCTAG ACTTTGAATT GGATACTTTT ATTGCATGTA GTACATTCTT TGGACCGTCA
    TCTGTCAATG AGGTAGAGCT ACTACCTTTG AAAGGTTATT TCCCTTCCAA CTGGCCCACT
    AACACATTGG TCCATGCATT ATTGGTTTGT AATGCTAGCA CGGAGCTGAC CACTTTGAAA
    AATATTCAGG ACTACTTTAA TCCAGCTACT CTGCCTCTAA CACAGAACCT GTTAACAATG
    TCTTCATCAA CTATAGTGAG CAACAAGAGA GTCAATAAGA GAAAATTTAT CCCACCAGCC
    TTCTCAAATA TCAATACAAA ATTTGAACTG CTCAGTATAG GAGCAACGCT GCAGTTAGCT
    GGTGAGTTAA TTCACACACA CAAGTTAAAC AAGGATCAAG CTACAGCCCT AATTCAGATA
    GCGCAAATGA TGGCATCACA AGGAAGTGCT GAAGAAGTGA AGGAACTGGG AACTCACACC
    CTCCCAATCA CGATCATACA TGGTGTTTTT GGAGCAGGAA AAAGTTATTT GCTGGCAGTG
    GTGATTTTGT TTTTTGTTCA GCTATTTGAA AAGAGTGAAG CTCTTGCAGT TGGAAATGGA
    AGACCTTGGA AACTTCTGAT TTCTTCTTCT ACTAATGTAG CTGTTGACAG AGTACTTCTT
    GGGCTTCTTA GTCTTGGGTT TGAAAAGTTT GTCAGAGTTG GGAGTGTCAG GAAGATTGCC
    AAGCCAATTT TACCTTACAG TTTGCATGCT GGCTCAGAAA ATGAAAATGA ACAATTAAAA
    GAACTGCATA CACTCATGAA GGAAGACCTG ACTCCTGTGG AAAGAGTCTA TGTGAGAAAA
    AGTATTGAGC AACATAAACT GGGGACCAAT AAAACCCTGC TGAAGCAGGT TCGAGTAGTT
    GGAGTTACCT GTGCAGCCTG CCCATTTCCA TGCATGAATG ACCTTAAATT TCCTGTGGTT
    GTACTGGATG AGTGTAGCCA AATAACCGAA CCAGCTTCTC TCCTTCCCAT TGCAAGGTTT
    GAGTGTGAAA AGCTTATTCT TGTTGGAGAT CCCAAACAGC TTCCTCCTAC TATTCAGGGT
    TCTGATGCAG CTCATGAAAA TGGACTGGAA CAAACTCTTT TTGATCGACT TTGCTTAATG
    GGTCACAAGC CAGTTCTTTT GCGAACCCAG TACCGTTGTC ACCCTACAAT CAGTGCTATT
    GCTAATGATC TGTTTTATGA AGGAAACCTC GTGAATGGAG TTTCAGAAAC AGAGAGGAGC
    CCTTTATTGG AATGGCTTCC AACCCTGTGT TTCTATAACA TTCAAGGGCT GGAACAGATC
    GAAAGAGATA ACAGCTTTCA TAACGTAGCA GAAGCTGCTT TTACACTCAA GCTGATTCAA
    TCACTGATTG TGAGTGGAAT AGCAGGCTCT ATGATTGGGG TGATAACATT ATACAAATCC
    CAGATGTACA AGCTTTGTCA CTTACTCACT GCACTGGACT TTGACCATCC TGGTAATGTG
    AAAGCTGTGC AGGTGTCCAC AGTAGATGCT TTTCAGGGCG CTGAAAAGGA GATCATCATT
    CTGTCCTGTG TGAGGACAAG ACAAGTAGGA TTTATTGATT CAGAACAAAG AATGAATGTT
    GCATTGACCA GAGGAAGGAG ACATTTGTTG ATTGTGGGAA ATTTGGCCTG TTTGAGGAAA
    AATCGACTAT GGGGGCGTGT GATCCAACAC TGTGAAGGCA GGGAAGATGG ATTGCAACAT
    GCAAGCCAAT ATGAACCACA GCTCAACCAT CTCCTTAAAG ATTATTTTGA AAAACAAGCA
    GAAGAAAAAC AAAAGAAAAA GAATGAAAAA GATAAATCCA AAGATAAATC TCATTTACAA
    AAAGAAACAG TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020957324.1
    CDS405..6059
    Translation

    Target ORF information:

    RefSeq Version XM_021101665.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa zinc finger GRF-type containing 1 (ZGRF1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021101665.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGAAAGCC AGGAATTTAT TGTCCTATAT ACTCATCAAA AGATGAAGAA GTCAAAAGTA 
    TGGCAAGATG GAATTCTGAA GATCACTCAC GTAGGAAACA AAGCCATTTT ATATGATGAC
    AAAGGACAGT GTTTGGAGAG TTTATTTCTT AAGTGCCTTG AGGTGAAACC TGGAGATGAT
    TTAGAAAGTG ATCGATACTT AATCACAGTT GAGGAGGTTA AAGTTGCTGG AAGCATAGCT
    GTTAAACAAG ATATCATTAA AGGAGCACCT CCATTAAATT CAAAGAGCTT TATACCTTCT
    GGCCGGTCCC TTGGGTGTCA ACCTGCTGGT TTAAAAAGGA AATTTACTGG TTTTCAAGGA
    CCACGGCAAG TGCCAAAGAA AATGGCTATT ACAGAAAATA GTGAATCAGC TGCTTCACTT
    GAGGCTAAGG AAGCTGGTCC TGTTTTTCTT TCTCCAGTCT ACAGCACACC TCCTTTGTTT
    CCTACTGTTG GCAAAAAAGA TATAAATTAT ATGCCAACAG ACCCTGACAA CCAGGACAGA
    GAGAGAAATG GCATAGCTTT TTCTTCGGTT GTCTCTACTC CATCCTTCAA GATTAACCCA
    GAAATGCTAT GTGAAGAAAA TTATTTTTGC TCTACTGTCA ATTCTGTAAA TAAGCATTCA
    GGTTCTTTAC TGACCGATGA GCTTATGAAA AGAGATTGTT TGGTATCTCA CAATTCAGGA
    GTTTCACAGA ACATTAGAAG TAAAGCCCAG ATATTGGCTC TTCTGAAATC CAAATCCACT
    GGTACATCTA AAGAACCAAA TTCTGAGATT ACAGGACATT TCCCTCTGAT ACAACCACAA
    GGAAGTTTAA AAATTCCTGC TAAACTTGAG TGCTTAATTG AACAGGAAGA ACACACTCAG
    GTGAAGAGCA CAGAAAGTTT ATACTGCCAA CATCAATCAA AAAATACCAT GAGAAATAAA
    AGCCGGTGGG CCATGTATTT ATCCTCACAG AGTTCACCTA CACATTCTTC TACAGTAGAT
    GGAAATAATA CAGGAAAAAA TCCTGAGGCC GAGGGAGGTA ATATAAATTT AAATTTGAAA
    GACGCTTTGG TGCAAAAAAG CATGCGGTTT TTAGAAACAT GTACTGAAGA GGGAAAAAAG
    TATAATGAAG ACAAGCCAGT AGTTGATAAT GATCAATCCT GGAATCAAAA AGTAAAATTA
    GAATTTCCTT CATTCTGTGA AAGCAGTGCC TTACCAGTTA CTTGTGACAA CGTAGAAAAC
    GACAGCTTAT TATCAGAAAA TGACAGCTTA TTATCAGAAA ATGACATTCA AGAAAATAAT
    AAAAGACTTT TTAATCAAAA TGACCAGATG GGCATAAAAG GATCAATTCT CATTACAGAA
    AAAGCTCAGG AGATAACCAT GTATGGAACC CCAGCTAAGG AGTATAAACA TCTGCAGATT
    GAATCTTCAC TAAGTAACGA TTTTAGGGTC TCTAATGGCA TTGCTGACAT GTTTTCTAAA
    AGTAATGCTG ATAATGAAAA TCTGAACAGT ATTCATGAAC CACTTGTGGA GATTACTTTT
    AATCTGAACA ATTTTGAAAC CAGTGACTCT GAGGAGGAAT CACAGGAAAG CAGCAGAATT
    TCCCACGATT CAGAGGGTTG GGTGAAGGAA ACTTTAGTTA AAGACAATTC AAGCTGTGAG
    AATATAAACT GTGCAGAAGT TGGCAGTGAC CATTTGCCTC TCTTAACGTC TAGTGGGGAC
    AGACTTGCAG AGACATTTTC CATGAAAGAG ACTCTGCTAT CACAATTTTG TGATAAAACT
    TCTGTAGGTT TTGACACAGG ACCTTGGAAA GCTGGAAACA CTAGAAAAGA AATAGAGGAG
    GAGTACGGTG ACACCTCAAA CAATCTTGAC TCATCTTTAG AGTGGACTGA AGATGCAAAT
    GTAGGTAGTA ATGAAGGCAC TAATAAATCT ACTCAGGAAG TCACAGTTAA CTATGATTTT
    GCTTCTCTCC CAAATGAAAG TATAAACACA AATTTACATA TCCCTCACTC TTTAAACACA
    GCCACTAATC AGGATCCTGA AAACAATCCA TGGTCACAAC AGGCTCAGCC TCAGCCTTTT
    ATTATGGGAA CTGACTTACA CAAAAATACT GAACAGGTTT TACCATTAAC TTCTAGCAGT
    GGCAACGGCA CCCAACTATT AAATGCCAGT CAGAATCACT CTGAAGAATT TCTTGCACTT
    GATAAATCAC ATACCCAAGT TTCCAATTCT TCGTTTTACC CTCTGGGAGT AAAATATCCT
    ATATCTAAAG ACACAGAATC ACATATTCTG GAATCTGAAG ATTTGGGAAG GACTAGAAGT
    TTACCTCATG ACAATACTGA CATAGAAACT GCCAGAGAGA GCAAACAGTA CTGGAATACT
    CCTAAAAATT CTTCAGAACT TTCTGGATTA GAAAATAGCA TTTCCCTATT AAAATCATTG
    TCTGAACACA GTACTGCTTT AGAAGGCTTG GAAATATTGA AAAAGAAACA TACCTCCTTT
    AAGCAACAAG GAACTCTGCA GACGTATGAT CCAGAAAGCA GTCCTGAAGC TAGGAAACCA
    TTGACTACAA TAGTTTCACA AGCTATACCA AAGTCTCATC CGAACCAGGA TCCTCAACAG
    ATGATAAAAG AAAATGAAGT TAAACCAAGT GAATCCCTCA CCAAGTTTTT TCCCTTGGGC
    AGTGAAGAAG AGACTGACTC CCGAGCTCTC ATTCCTAAAC AAATGGAGAG AAAAACCTGT
    GACCCTAAGC CTGTTGAGTT TCAAGGGCAC CAAGTGAAAG GATCAGCTGC TAGTGCTGTG
    ATGGTCAGAG GACACAGCTC CCAGTTTGAA TGCAGTCAAT TTCCAGATAG CGTGTATGAA
    AACTGCATGA CAAATACTTG TTTTTTGACA CCTAAGTTGC CTAGCACTTG TATGCAGATT
    GACTTCTTGC AGTTGCCAGA TGAAGAAAAC TTGATAAAAG GAGATTCCAG CTTTAACACT
    GAAGACAATT TTCAAGTGAT AGCTGAGCCT AATAAGAATT CAGTTAAAAA TGATCTAATG
    AAAAAGTCTC TGGAGAATAA GAACCTTCAA AGGCTTTCAT TAGAGATAAG TGACTGTGAG
    CTGTGCTTCC CAAGTGGGGA GAAAGTAAAA TCTGCATATC TTCCCCAAAG GCAAATTCAC
    ATACCAGCTG TTTTTCAGTC TCCTGCTCAT TATAAACAGA TTTTTACATC TTGTCTCATA
    GAACATCTAA ATATATTGCT GTTTGGGTTG GCACAAAGGC TGCACAAAGC TCTTTCAAAA
    GTTGACATAT CATTTTACTC ATCAAACGGA GACAAACGGA AAAATGTAGA AAATAATGTG
    CCATCATGCC ATCATCATCA CCCTGCAAAA CTTGTCATGG TTAAAAAGGA AGGTCCAAAT
    AAGGGTCGTC TCTTTTATGC TTGTGATGGA CCCAAAGCTG ACCAATGTAA ATTTTTCAAG
    TGGCTTGATG AAGTGACCCC AGGATATTTA ACACAGGAAG AATCACTACC TAGCATGGTT
    TTAAGTGATA TAAAAAGTAT TGGCTTGTAC TTAAGAAGTC AAAAGATACC CCTCTATGAG
    GAATGCCAAC TTTTGGTGAG AAAAGGATTT GATTTTCAAA GAAAACGGTA TGGTAAACTA
    AAGAAGTTTA CAACTGTAGA TCCTGAATTT TATAACGAAC CTAAGAGCAG ACTTTATCTT
    AAGCTGAGTC GTAAGGAAAG TTCATCTGCT TATAGTAAAG ATGATCTTTG GGTGGTTTCA
    AAAACCCTAG ACTTTGAATT GGATACTTTT ATTGCATGTA GTACATTCTT TGGACCGTCA
    TCTGTCAATG AGGTAGAGCT ACTACCTTTG AAAGGTTATT TCCCTTCCAA CTGGCCCACT
    AACACATTGG TCCATGCATT ATTGGTTTGT AATGCTAGCA CGGAGCTGAC CACTTTGAAA
    AATATTCAGG ACTACTTTAA TCCAGCTACT CTGCCTCTAA CACAGAACCT GTTAACAATG
    TCTTCATCAA CTATAGTGAG CAACAAGAGA GTCAATAAGA GAAAATTTAT CCCACCAGCC
    TTCTCAAATA TCAATACAAA ATTTGAACTG CTCAGTATAG GAGCAACGCT GCAGTTAGCT
    GGTGAGTTAA TTCACACACA CAAGTTAAAC AAGGATCAAG CTACAGCCCT AATTCAGATA
    GCGCAAATGA TGGCATCACA AGGAAGTGCT GAAGAAGTGA AGGAACTGGG AACTCACACC
    CTCCCAATCA CGATCATACA TGGTGTTTTT GGAGCAGGAA AAAGTTATTT GCTGGCAGTG
    GTGATTTTGT TTTTTGTTCA GCTATTTGAA AAGAGTGAAG CTCTTGCAGT TGGAAATGGA
    AGACCTTGGA AACTTCTGAT TTCTTCTTCT ACTAATGTAG CTGTTGACAG AGTACTTCTT
    GGGCTTCTTA GTCTTGGGTT TGAAAAGTTT GTCAGAGTTG GGAGTGTCAG GAAGATTGCC
    AAGCCAATTT TACCTTACAG TTTGCATGCT GGCTCAGAAA ATGAAAATGA ACAATTAAAA
    GAACTGCATA CACTCATGAA GGAAGACCTG ACTCCTGTGG AAAGAGTCTA TGTGAGAAAA
    AGTATTGAGC AACATAAACT GGGGACCAAT AAAACCCTGC TGAAGCAGGT TCGAGTAGTT
    GGAGTTACCT GTGCAGCCTG CCCATTTCCA TGCATGAATG ACCTTAAATT TCCTGTGGTT
    GTACTGGATG AGTGTAGCCA AATAACCGAA CCAGCTTCTC TCCTTCCCAT TGCAAGGTTT
    GAGTGTGAAA AGCTTATTCT TGTTGGAGAT CCCAAACAGC TTCCTCCTAC TATTCAGGGT
    TCTGATGCAG CTCATGAAAA TGGACTGGAA CAAACTCTTT TTGATCGACT TTGCTTAATG
    GGTCACAAGC CAGTTCTTTT GCGAACCCAG TACCGTTGTC ACCCTACAAT CAGTGCTATT
    GCTAATGATC TGTTTTATGA AGGAAACCTC GTGAATGGAG TTTCAGAAAC AGAGAGGAGC
    CCTTTATTGG AATGGCTTCC AACCCTGTGT TTCTATAACA TTCAAGGGCT GGAACAGATC
    GAAAGAGATA ACAGCTTTCA TAACGTAGCA GAAGCTGCTT TTACACTCAA GCTGATTCAA
    TCACTGATTG TGAGTGGAAT AGCAGGCTCT ATGATTGGGG TGATAACATT ATACAAATCC
    CAGATGTACA AGCTTTGTCA CTTACTCACT GCACTGGACT TTGACCATCC TGGTAATGTG
    AAAGCTGTGC AGGTGTCCAC AGTAGATGCT TTTCAGGGCG CTGAAAAGGA GATCATCATT
    CTGTCCTGTG TGAGGACAAG ACAAGTAGGA TTTATTGATT CAGAACAAAG AATGAATGTT
    GCATTGACCA GAGGAAGGAG ACATTTGTTG ATTGTGGGAA ATTTGGCCTG TTTGAGGAAA
    AATCGACTAT GGGGGCGTGT GATCCAACAC TGTGAAGGCA GGGAAGATGG ATTGCAACAT
    GCAAGCCAAT ATGAACCACA GCTCAACCAT CTCCTTAAAG ATTATTTTGA AAAACAAGCA
    GAAGAAAAAC AAAAGAAAAA GAATGAAAAA GATAAATCCA AAGATAAATC TCATTTACAA
    AAAGAAACAG TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe224829
    Clone ID Related Accession (Same CDS sequence) XM_021101666.1
    Accession Version XM_021101666.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5511bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product protein ZGRF1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010450.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGAAAGCC AGGAATTTAT TGTCCTATAT ACTCATCAAA AGATGAAGAA GTCAAAAGTA 
    TGGCAAGATG GAATTCTGAA GATCACTCAC GTAGGAAACA AAGCCATTTT ATATGATGAC
    AAAGGACAGT GTTTGGAGAG TTTATTTCTT AAGTGCCTTG AGGTGAAACC TGGAGATGAT
    TTAGAAAGTG ATCGATACTT AATCACAGTT GAGGAGGTTA AAGTTGCTGG AAGCATAGCT
    GTTAAACAAG ATATCATTAA AGGAGCACCT CCATTAAATT CAAAGAGCTT TATACCTTCT
    GGCCGGTCCC TTGGGTGTCA ACCTGCTGGT TTAAAAAGGA AATTTACTGG TTTTCAAGGA
    CCACGGCAAG TGCCAAAGAA AATGGCTATT ACAGAAAATA GTGAATCAGC TGCTTCACTT
    GAGGCTAAGG AAGCTGGTCC TGTTTTTCTT TCTCCAGTCT ACAGCACACC TCCTTTGTTT
    CCTACTGTTG GCAAAAAAGA TATAAATTAT ATGCCAACAG ACCCTGACAA CCAGGACAGA
    GAGAGAAATG GCATAGCTTT TTCTTCGGTT GTCTCTACTC CATCCTTCAA GATTAACCCA
    GAAATGCTAT GTGAAGAAAA TTATTTTTGC TCTACTGTCA ATTCTGTAAA TAAGCATTCA
    GGTTCTTTAC TGACCGATGA GCTTATGAAA AGAGATTGTT TGGTATCTCA CAATTCAGGA
    GTTTCACAGA ACATTAGAAG TAAAGCCCAG ATATTGGCTC TTCTGAAATC CAAATCCACT
    GGTACATCTA AAGAACCAAA TTCTGAGATT ACAGGACATT TCCCTCTGAT ACAACCACAA
    GGAAGTTTAA AAATTCCTGC TAAACTTGAG TGCTTAATTG AACAGGAAGA ACACACTCAG
    GTGAAGAGCA CAGAAAGTTT ATACTGCCAA CATCAATCAA AAAATACCAT GAGAAATAAA
    AGCCGGTGGG CCATGTATTT ATCCTCACAG AGTTCACCTA CACATTCTTC TACAGTAGAT
    GGAAATAATA CAGGAAAAAA TCCTGAGGCC GAGGGAGGTA ATATAAATTT AAATTTGAAA
    GACGCTTTGG TGCAAAAAAG CATGCGGTTT TTAGAAACAT GTACTGAAGA GGGAAAAAAG
    TATAATGAAG ACAAGCCAGT AGTTGATAAT GATCAATCCT GGAATCAAAA AGTAAAATTA
    GAATTTCCTT CATTCTGTGA AAGCAGTGCC TTACCAGTTA CTTGTGACAA CGTAGAAAAC
    GACAGCTTAT TATCAGAAAA TGACAGCTTA TTATCAGAAA ATGACATTCA AGAAAATAAT
    AAAAGACTTT TTAATCAAAA TGACCAGATG GGCATAAAAG GATCAATTCT CATTACAGAA
    AAAGCTCAGG AGATAACCAT GTATGGAACC CCAGCTAAGG AGTATAAACA TCTGCAGATT
    GAATCTTCAC TAAGTAACGA TTTTAGGGTC TCTAATGGCA TTGCTGACAT GTTTTCTAAA
    AGTAATGCTG ATAATGAAAA TCTGAACAGT ATTCATGAAC CACTTGTGGA GATTACTTTT
    AATCTGAACA ATTTTGAAAC CAGTGACTCT GAGGAGGAAT CACAGGAAAG CAGCAGAATT
    TCCCACGATT CAGAGGGTTG GGTGAAGGAA ACTTTAGTTA AAGACAATTC AAGCTGTGAG
    AATATAAACT GTGCAGAAGT TGGCAGTGAC CATTTGCCTC TCTTAACGTC TAGTGGGGAC
    AGACTTGCAG AGACATTTTC CATGAAAGAG ACTCTGCTAT CACAATTTTG TGATAAAACT
    TCTGTAGGTT TTGACACAGG ACCTTGGAAA GCTGGAAACA CTAGAAAAGA AATAGAGGAG
    GAGTACGGTG ACACCTCAAA CAATCTTGAC TCATCTTTAG AGTGGACTGA AGATGCAAAT
    GTAGGTAGTA ATGAAGGCAC TAATAAATCT ACTCAGGAAG TCACAGTTAA CTATGATTTT
    GCTTCTCTCC CAAATGAAAG TATAAACACA AATTTACATA TCCCTCACTC TTTAAACACA
    GCCACTAATC AGGATCCTGA AAACAATCCA TGGTCACAAC AGGCTCAGCC TCAGCCTTTT
    ATTATGGGAA CTGACTTACA CAAAAATACT GAACAGGTTT TACCATTAAC TTCTAGCAGT
    GGCAACGGCA CCCAACTATT AAATGCCAGT CAGAATCACT CTGAAGAATT TCTTGCACTT
    GATAAATCAC ATACCCAAGT TTCCAATTCT TCGTTTTACC CTCTGGGAGT AAAATATCCT
    ATATCTAAAG ACACAGAATC ACATATTCTG GAATCTGAAG ATTTGGGAAG GACTAGAAGT
    TTACCTCATG ACAATACTGA CATAGAAACT GCCAGAGAGA GCAAACAGTA CTGGAATACT
    CCTAAAAATT CTTCAGAACT TTCTGGATTA GAAAATAGCA TTTCCCTATT AAAATCATTG
    TCTGAACACA GTACTGCTTT AGAAGGCTTG GAAATATTGA AAAAGAAACA TACCTCCTTT
    AAGCAACAAG GAACTCTGCA GACGTATGAT CCAGAAAGCA GTCCTGAAGC TAGGAAACCA
    TTGACTACAA TAGTTTCACA AGCTATACCA AAGTCTCATC CGAACCAGGA TCCTCAACAG
    ATGATAAAAG AAAATGAAGT TAAACCAAGT GAATCCCTCA CCAAGTTTTT TCCCTTGGGC
    AGTGAAGAAG AGACTGACTC CCGAGCTCTC ATTCCTAAAC AAATGGAGAG AAAAACCTGT
    GACCCTAAGC CTGTTGAGTT TCAAGGGCAC CAAGTGAAAG GATCAGCTGC TAGTGCTGTG
    ATGGTCAGAG GACACAGCTC CCAGTTTGAA TGCAGTCAAT TTCCAGATAG CGTGTATGAA
    AACTGCATGA CAAATACTTG TTTTTTGACA CCTAAGTTGC CTAGCACTTG TATGCAGATT
    GACTTCTTGC AGTTGCCAGA TGAAGAAAAC TTGATAAAAG GAGATTCCAG CTTTAACACT
    GAAGACAATT TTCAAGTGAT AGCTGAGCCT AATAAGAATT CAGTTAAAAA TGATCTAATG
    AAAAAGTCTC TGGAGAATAA GAACCTTCAA AGGCTTTCAT TAGAGATAAG TGACTGTGAG
    CTGTGCTTCC CAAGTGGGGA GAAAGTAAAA TCTGCATATC TTCCCCAAAG GCAAATTCAC
    ATACCAGCTG TTTTTCAGTC TCCTGCTCAT TATAAACAGA TTTTTACATC TTGTCTCATA
    GAACATCTAA ATATATTGCT GTTTGGGTTG GCACAAAGGC TGCACAAAGC TCTTTCAAAA
    GTTGACATAT CATTTTACTC ATCAAACGGA GACAAACGGA AAAATGTAGA AAATAATGTG
    CCATCATGCC ATCATCATCA CCCTGCAAAA CTTGTCATGG TTAAAAAGGA AGGTCCAAAT
    AAGGGTCGTC TCTTTTATGC TTGTGATGGA CCCAAAGCTG ACCAATGTAA ATTTTTCAAG
    TGGCTTGATG AAGTGACCCC AGGATATTTA ACACAGGAAG AATCACTACC TAGCATGGTT
    TTAAGTGATA TAAAAAGTAT TGGCTTGTAC TTAAGAAGTC AAAAGATACC CCTCTATGAG
    GAATGCCAAC TTTTGGTGAG AAAAGGATTT GATTTTCAAA GAAAACGGTA TGGTAAACTA
    AAGAAGTTTA CAACTGTAGA TCCTGAATTT TATAACGAAC CTAAGAGCAG ACTTTATCTT
    AAGCTGAGTC GTAAGGAAAG TTCATCTGCT TATAGTAAAG CATTGGTCCA TGCATTATTG
    GTTTGTAATG CTAGCACGGA GCTGACCACT TTGAAAAATA TTCAGGACTA CTTTAATCCA
    GCTACTCTGC CTCTAACACA GAACCTGTTA ACAATGTCTT CATCAACTAT AGTGAGCAAC
    AAGAGAGTCA ATAAGAGAAA ATTTATCCCA CCAGCCTTCT CAAATATCAA TACAAAATTT
    GAACTGCTCA GTATAGGAGC AACGCTGCAG TTAGCTGGTG AGTTAATTCA CACACACAAG
    TTAAACAAGG ATCAAGCTAC AGCCCTAATT CAGATAGCGC AAATGATGGC ATCACAAGGA
    AGTGCTGAAG AAGTGAAGGA ACTGGGAACT CACACCCTCC CAATCACGAT CATACATGGT
    GTTTTTGGAG CAGGAAAAAG TTATTTGCTG GCAGTGGTGA TTTTGTTTTT TGTTCAGCTA
    TTTGAAAAGA GTGAAGCTCT TGCAGTTGGA AATGGAAGAC CTTGGAAACT TCTGATTTCT
    TCTTCTACTA ATGTAGCTGT TGACAGAGTA CTTCTTGGGC TTCTTAGTCT TGGGTTTGAA
    AAGTTTGTCA GAGTTGGGAG TGTCAGGAAG ATTGCCAAGC CAATTTTACC TTACAGTTTG
    CATGCTGGCT CAGAAAATGA AAATGAACAA TTAAAAGAAC TGCATACACT CATGAAGGAA
    GACCTGACTC CTGTGGAAAG AGTCTATGTG AGAAAAAGTA TTGAGCAACA TAAACTGGGG
    ACCAATAAAA CCCTGCTGAA GCAGGTTCGA GTAGTTGGAG TTACCTGTGC AGCCTGCCCA
    TTTCCATGCA TGAATGACCT TAAATTTCCT GTGGTTGTAC TGGATGAGTG TAGCCAAATA
    ACCGAACCAG CTTCTCTCCT TCCCATTGCA AGGTTTGAGT GTGAAAAGCT TATTCTTGTT
    GGAGATCCCA AACAGCTTCC TCCTACTATT CAGGGTTCTG ATGCAGCTCA TGAAAATGGA
    CTGGAACAAA CTCTTTTTGA TCGACTTTGC TTAATGGGTC ACAAGCCAGT TCTTTTGCGA
    ACCCAGTACC GTTGTCACCC TACAATCAGT GCTATTGCTA ATGATCTGTT TTATGAAGGA
    AACCTCGTGA ATGGAGTTTC AGAAACAGAG AGGAGCCCTT TATTGGAATG GCTTCCAACC
    CTGTGTTTCT ATAACATTCA AGGGCTGGAA CAGATCGAAA GAGATAACAG CTTTCATAAC
    GTAGCAGAAG CTGCTTTTAC ACTCAAGCTG ATTCAATCAC TGATTGTGAG TGGAATAGCA
    GGCTCTATGA TTGGGGTGAT AACATTATAC AAATCCCAGA TGTACAAGCT TTGTCACTTA
    CTCACTGCAC TGGACTTTGA CCATCCTGGT AATGTGAAAG CTGTGCAGGT GTCCACAGTA
    GATGCTTTTC AGGGCGCTGA AAAGGAGATC ATCATTCTGT CCTGTGTGAG GACAAGACAA
    GTAGGATTTA TTGATTCAGA ACAAAGAATG AATGTTGCAT TGACCAGAGG AAGGAGACAT
    TTGTTGATTG TGGGAAATTT GGCCTGTTTG AGGAAAAATC GACTATGGGG GCGTGTGATC
    CAACACTGTG AAGGCAGGGA AGATGGATTG CAACATGCAA GCCAATATGA ACCACAGCTC
    AACCATCTCC TTAAAGATTA TTTTGAAAAA CAAGCAGAAG AAAAACAAAA GAAAAAGAAT
    GAAAAAGATA AATCCAAAGA TAAATCTCAT TTACAAAAAG AAACAGTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020957325.1
    CDS405..5915
    Translation

    Target ORF information:

    RefSeq Version XM_021101666.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa zinc finger GRF-type containing 1 (ZGRF1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021101666.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGAAAGCC AGGAATTTAT TGTCCTATAT ACTCATCAAA AGATGAAGAA GTCAAAAGTA 
    TGGCAAGATG GAATTCTGAA GATCACTCAC GTAGGAAACA AAGCCATTTT ATATGATGAC
    AAAGGACAGT GTTTGGAGAG TTTATTTCTT AAGTGCCTTG AGGTGAAACC TGGAGATGAT
    TTAGAAAGTG ATCGATACTT AATCACAGTT GAGGAGGTTA AAGTTGCTGG AAGCATAGCT
    GTTAAACAAG ATATCATTAA AGGAGCACCT CCATTAAATT CAAAGAGCTT TATACCTTCT
    GGCCGGTCCC TTGGGTGTCA ACCTGCTGGT TTAAAAAGGA AATTTACTGG TTTTCAAGGA
    CCACGGCAAG TGCCAAAGAA AATGGCTATT ACAGAAAATA GTGAATCAGC TGCTTCACTT
    GAGGCTAAGG AAGCTGGTCC TGTTTTTCTT TCTCCAGTCT ACAGCACACC TCCTTTGTTT
    CCTACTGTTG GCAAAAAAGA TATAAATTAT ATGCCAACAG ACCCTGACAA CCAGGACAGA
    GAGAGAAATG GCATAGCTTT TTCTTCGGTT GTCTCTACTC CATCCTTCAA GATTAACCCA
    GAAATGCTAT GTGAAGAAAA TTATTTTTGC TCTACTGTCA ATTCTGTAAA TAAGCATTCA
    GGTTCTTTAC TGACCGATGA GCTTATGAAA AGAGATTGTT TGGTATCTCA CAATTCAGGA
    GTTTCACAGA ACATTAGAAG TAAAGCCCAG ATATTGGCTC TTCTGAAATC CAAATCCACT
    GGTACATCTA AAGAACCAAA TTCTGAGATT ACAGGACATT TCCCTCTGAT ACAACCACAA
    GGAAGTTTAA AAATTCCTGC TAAACTTGAG TGCTTAATTG AACAGGAAGA ACACACTCAG
    GTGAAGAGCA CAGAAAGTTT ATACTGCCAA CATCAATCAA AAAATACCAT GAGAAATAAA
    AGCCGGTGGG CCATGTATTT ATCCTCACAG AGTTCACCTA CACATTCTTC TACAGTAGAT
    GGAAATAATA CAGGAAAAAA TCCTGAGGCC GAGGGAGGTA ATATAAATTT AAATTTGAAA
    GACGCTTTGG TGCAAAAAAG CATGCGGTTT TTAGAAACAT GTACTGAAGA GGGAAAAAAG
    TATAATGAAG ACAAGCCAGT AGTTGATAAT GATCAATCCT GGAATCAAAA AGTAAAATTA
    GAATTTCCTT CATTCTGTGA AAGCAGTGCC TTACCAGTTA CTTGTGACAA CGTAGAAAAC
    GACAGCTTAT TATCAGAAAA TGACAGCTTA TTATCAGAAA ATGACATTCA AGAAAATAAT
    AAAAGACTTT TTAATCAAAA TGACCAGATG GGCATAAAAG GATCAATTCT CATTACAGAA
    AAAGCTCAGG AGATAACCAT GTATGGAACC CCAGCTAAGG AGTATAAACA TCTGCAGATT
    GAATCTTCAC TAAGTAACGA TTTTAGGGTC TCTAATGGCA TTGCTGACAT GTTTTCTAAA
    AGTAATGCTG ATAATGAAAA TCTGAACAGT ATTCATGAAC CACTTGTGGA GATTACTTTT
    AATCTGAACA ATTTTGAAAC CAGTGACTCT GAGGAGGAAT CACAGGAAAG CAGCAGAATT
    TCCCACGATT CAGAGGGTTG GGTGAAGGAA ACTTTAGTTA AAGACAATTC AAGCTGTGAG
    AATATAAACT GTGCAGAAGT TGGCAGTGAC CATTTGCCTC TCTTAACGTC TAGTGGGGAC
    AGACTTGCAG AGACATTTTC CATGAAAGAG ACTCTGCTAT CACAATTTTG TGATAAAACT
    TCTGTAGGTT TTGACACAGG ACCTTGGAAA GCTGGAAACA CTAGAAAAGA AATAGAGGAG
    GAGTACGGTG ACACCTCAAA CAATCTTGAC TCATCTTTAG AGTGGACTGA AGATGCAAAT
    GTAGGTAGTA ATGAAGGCAC TAATAAATCT ACTCAGGAAG TCACAGTTAA CTATGATTTT
    GCTTCTCTCC CAAATGAAAG TATAAACACA AATTTACATA TCCCTCACTC TTTAAACACA
    GCCACTAATC AGGATCCTGA AAACAATCCA TGGTCACAAC AGGCTCAGCC TCAGCCTTTT
    ATTATGGGAA CTGACTTACA CAAAAATACT GAACAGGTTT TACCATTAAC TTCTAGCAGT
    GGCAACGGCA CCCAACTATT AAATGCCAGT CAGAATCACT CTGAAGAATT TCTTGCACTT
    GATAAATCAC ATACCCAAGT TTCCAATTCT TCGTTTTACC CTCTGGGAGT AAAATATCCT
    ATATCTAAAG ACACAGAATC ACATATTCTG GAATCTGAAG ATTTGGGAAG GACTAGAAGT
    TTACCTCATG ACAATACTGA CATAGAAACT GCCAGAGAGA GCAAACAGTA CTGGAATACT
    CCTAAAAATT CTTCAGAACT TTCTGGATTA GAAAATAGCA TTTCCCTATT AAAATCATTG
    TCTGAACACA GTACTGCTTT AGAAGGCTTG GAAATATTGA AAAAGAAACA TACCTCCTTT
    AAGCAACAAG GAACTCTGCA GACGTATGAT CCAGAAAGCA GTCCTGAAGC TAGGAAACCA
    TTGACTACAA TAGTTTCACA AGCTATACCA AAGTCTCATC CGAACCAGGA TCCTCAACAG
    ATGATAAAAG AAAATGAAGT TAAACCAAGT GAATCCCTCA CCAAGTTTTT TCCCTTGGGC
    AGTGAAGAAG AGACTGACTC CCGAGCTCTC ATTCCTAAAC AAATGGAGAG AAAAACCTGT
    GACCCTAAGC CTGTTGAGTT TCAAGGGCAC CAAGTGAAAG GATCAGCTGC TAGTGCTGTG
    ATGGTCAGAG GACACAGCTC CCAGTTTGAA TGCAGTCAAT TTCCAGATAG CGTGTATGAA
    AACTGCATGA CAAATACTTG TTTTTTGACA CCTAAGTTGC CTAGCACTTG TATGCAGATT
    GACTTCTTGC AGTTGCCAGA TGAAGAAAAC TTGATAAAAG GAGATTCCAG CTTTAACACT
    GAAGACAATT TTCAAGTGAT AGCTGAGCCT AATAAGAATT CAGTTAAAAA TGATCTAATG
    AAAAAGTCTC TGGAGAATAA GAACCTTCAA AGGCTTTCAT TAGAGATAAG TGACTGTGAG
    CTGTGCTTCC CAAGTGGGGA GAAAGTAAAA TCTGCATATC TTCCCCAAAG GCAAATTCAC
    ATACCAGCTG TTTTTCAGTC TCCTGCTCAT TATAAACAGA TTTTTACATC TTGTCTCATA
    GAACATCTAA ATATATTGCT GTTTGGGTTG GCACAAAGGC TGCACAAAGC TCTTTCAAAA
    GTTGACATAT CATTTTACTC ATCAAACGGA GACAAACGGA AAAATGTAGA AAATAATGTG
    CCATCATGCC ATCATCATCA CCCTGCAAAA CTTGTCATGG TTAAAAAGGA AGGTCCAAAT
    AAGGGTCGTC TCTTTTATGC TTGTGATGGA CCCAAAGCTG ACCAATGTAA ATTTTTCAAG
    TGGCTTGATG AAGTGACCCC AGGATATTTA ACACAGGAAG AATCACTACC TAGCATGGTT
    TTAAGTGATA TAAAAAGTAT TGGCTTGTAC TTAAGAAGTC AAAAGATACC CCTCTATGAG
    GAATGCCAAC TTTTGGTGAG AAAAGGATTT GATTTTCAAA GAAAACGGTA TGGTAAACTA
    AAGAAGTTTA CAACTGTAGA TCCTGAATTT TATAACGAAC CTAAGAGCAG ACTTTATCTT
    AAGCTGAGTC GTAAGGAAAG TTCATCTGCT TATAGTAAAG CATTGGTCCA TGCATTATTG
    GTTTGTAATG CTAGCACGGA GCTGACCACT TTGAAAAATA TTCAGGACTA CTTTAATCCA
    GCTACTCTGC CTCTAACACA GAACCTGTTA ACAATGTCTT CATCAACTAT AGTGAGCAAC
    AAGAGAGTCA ATAAGAGAAA ATTTATCCCA CCAGCCTTCT CAAATATCAA TACAAAATTT
    GAACTGCTCA GTATAGGAGC AACGCTGCAG TTAGCTGGTG AGTTAATTCA CACACACAAG
    TTAAACAAGG ATCAAGCTAC AGCCCTAATT CAGATAGCGC AAATGATGGC ATCACAAGGA
    AGTGCTGAAG AAGTGAAGGA ACTGGGAACT CACACCCTCC CAATCACGAT CATACATGGT
    GTTTTTGGAG CAGGAAAAAG TTATTTGCTG GCAGTGGTGA TTTTGTTTTT TGTTCAGCTA
    TTTGAAAAGA GTGAAGCTCT TGCAGTTGGA AATGGAAGAC CTTGGAAACT TCTGATTTCT
    TCTTCTACTA ATGTAGCTGT TGACAGAGTA CTTCTTGGGC TTCTTAGTCT TGGGTTTGAA
    AAGTTTGTCA GAGTTGGGAG TGTCAGGAAG ATTGCCAAGC CAATTTTACC TTACAGTTTG
    CATGCTGGCT CAGAAAATGA AAATGAACAA TTAAAAGAAC TGCATACACT CATGAAGGAA
    GACCTGACTC CTGTGGAAAG AGTCTATGTG AGAAAAAGTA TTGAGCAACA TAAACTGGGG
    ACCAATAAAA CCCTGCTGAA GCAGGTTCGA GTAGTTGGAG TTACCTGTGC AGCCTGCCCA
    TTTCCATGCA TGAATGACCT TAAATTTCCT GTGGTTGTAC TGGATGAGTG TAGCCAAATA
    ACCGAACCAG CTTCTCTCCT TCCCATTGCA AGGTTTGAGT GTGAAAAGCT TATTCTTGTT
    GGAGATCCCA AACAGCTTCC TCCTACTATT CAGGGTTCTG ATGCAGCTCA TGAAAATGGA
    CTGGAACAAA CTCTTTTTGA TCGACTTTGC TTAATGGGTC ACAAGCCAGT TCTTTTGCGA
    ACCCAGTACC GTTGTCACCC TACAATCAGT GCTATTGCTA ATGATCTGTT TTATGAAGGA
    AACCTCGTGA ATGGAGTTTC AGAAACAGAG AGGAGCCCTT TATTGGAATG GCTTCCAACC
    CTGTGTTTCT ATAACATTCA AGGGCTGGAA CAGATCGAAA GAGATAACAG CTTTCATAAC
    GTAGCAGAAG CTGCTTTTAC ACTCAAGCTG ATTCAATCAC TGATTGTGAG TGGAATAGCA
    GGCTCTATGA TTGGGGTGAT AACATTATAC AAATCCCAGA TGTACAAGCT TTGTCACTTA
    CTCACTGCAC TGGACTTTGA CCATCCTGGT AATGTGAAAG CTGTGCAGGT GTCCACAGTA
    GATGCTTTTC AGGGCGCTGA AAAGGAGATC ATCATTCTGT CCTGTGTGAG GACAAGACAA
    GTAGGATTTA TTGATTCAGA ACAAAGAATG AATGTTGCAT TGACCAGAGG AAGGAGACAT
    TTGTTGATTG TGGGAAATTT GGCCTGTTTG AGGAAAAATC GACTATGGGG GCGTGTGATC
    CAACACTGTG AAGGCAGGGA AGATGGATTG CAACATGCAA GCCAATATGA ACCACAGCTC
    AACCATCTCC TTAAAGATTA TTTTGAAAAA CAAGCAGAAG AAAAACAAAA GAAAAAGAAT
    GAAAAAGATA AATCCAAAGA TAAATCTCAT TTACAAAAAG AAACAGTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe224830
    Clone ID Related Accession (Same CDS sequence) XM_021101667.1
    Accession Version XM_021101667.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5472bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product protein ZGRF1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010450.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGAAAGCC AGGAATTTAT TGTCCTATAT ACTCATCAAA AGATGAAGAA GTCAAAAGTA 
    TGGCAAGATG GAATTCTGAA GATCACTCAC GTAGGAAACA AAGCCATTTT ATATGATGAC
    AAAGGACAGT GTTTGGAGAG TTTATTTCTT AAGTGCCTTG AGGTGAAACC TGGAGATGAT
    TTAGAAAGTG ATCGATACTT AATCACAGTT GAGGAGGTTA AAGTTGCTGG AAGCATAGCT
    GTTAAACAAG ATATCATTAA AGGAGCACCT CCATTAAATT CAAAGAGCTT TATACCTTCT
    GGCCGGTCCC TTGGGTGTCA ACCTGCTGGT TTAAAAAGGA AATTTACTGG TTTTCAAGGA
    CCACGGCAAG TGCCAAAGAA AATGGCTATT ACAGAAAATA GTGAATCAGC TGCTTCACTT
    GAGGCTAAGG AAGCTGGTCC TGTTTTTCTT TCTCCAGTCT ACAGCACACC TCCTTTGTTT
    CCTACTGTTG GCAAAAAAGA TATAAATTAT ATGCCAACAG ACCCTGACAA CCAGGACAGA
    GAGAGAAATG GCATAGCTTT TTCTTCGGTT GTCTCTACTC CATCCTTCAA GATTAACCCA
    GAAATGCTAT GTGAAGAAAA TTATTTTTGC TCTACTGTCA ATTCTGTAAA TAAGCATTCA
    GGTTCTTTAC TGACCGATGA GCTTATGAAA AGAGATTGTT TGGTATCTCA CAATTCAGGA
    GTTTCACAGA ACATTAGAAG TAAAGCCCAG ATATTGGCTC TTCTGAAATC CAAATCCACT
    GGTACATCTA AAGAACCAAA TTCTGAGATT ACAGGACATT TCCCTCTGAT ACAACCACAA
    GGAAGTTTAA AAATTCCTGC TAAACTTGAG TGCTTAATTG AACAGGAAGA ACACACTCAG
    GTGAAGAGCA CAGAAAGTTT ATACTGCCAA CATCAATCAA AAAATACCAT GAGAAATAAA
    AGCCGGTGGG CCATGTATTT ATCCTCACAG AGTTCACCTA CACATTCTTC TACAGTAGAT
    GGAAATAATA CAGGAAAAAA TCCTGAGGCC GAGGGAGGTA ATATAAATTT AAATTTGAAA
    GACGCTTTGG TGCAAAAAAG CATGCGGTTT TTAGAAACAT GTACTGAAGA GGGAAAAAAG
    TATAATGAAG ACAAGCCAGT AGTTGATAAT GATCAATCCT GGAATCAAAA AGTAAAATTA
    GAATTTCCTT CATTCTGTGA AAGCAGTGCC TTACCAGTTA CTTGTGACAA CGTAGAAAAC
    GACAGCTTAT TATCAGAAAA TGACAGCTTA TTATCAGAAA ATGACATTCA AGAAAATAAT
    AAAAGACTTT TTAATCAAAA TGACCAGATG GGCATAAAAG GATCAATTCT CATTACAGAA
    AAAGCTCAGG AGATAACCAT GTATGGAACC CCAGCTAAGG AGTATAAACA TCTGCAGATT
    GAATCTTCAC TAAGTAACGA TTTTAGGGTC TCTAATGGCA TTGCTGACAT GTTTTCTAAA
    AGTAATGCTG ATAATGAAAA TCTGAACAGT ATTCATGAAC CACTTGTGGA GATTACTTTT
    AATCTGAACA ATTTTGAAAC CAGTGACTCT GAGGAGGAAT CACAGGAAAG CAGCAGAATT
    TCCCACGATT CAGAGGGTTG GGTGAAGGAA ACTTTAGTTA AAGACAATTC AAGCTGTGAG
    AATATAAACT GTGCAGAAGT TGGCAGTGAC CATTTGCCTC TCTTAACGTC TAGTGGGGAC
    AGACTTGCAG AGACATTTTC CATGAAAGAG ACTCTGCTAT CACAATTTTG TGATAAAACT
    TCTGTAGGTT TTGACACAGG ACCTTGGAAA GCTGGAAACA CTAGAAAAGA AATAGAGGAG
    GAGTACGGTG ACACCTCAAA CAATCTTGAC TCATCTTTAG AGTGGACTGA AGATGCAAAT
    GTAGGTAGTA ATGAAGGCAC TAATAAATCT ACTCAGGAAG TCACAGTTAA CTATGATTTT
    GCTTCTCTCC CAAATGAAAG TATAAACACA AATTTACATA TCCCTCACTC TTTAAACACA
    GCCACTAATC AGGATCCTGA AAACAATCCA TGGTCACAAC AGGCTCAGCC TCAGCCTTTT
    ATTATGGGAA CTGACTTACA CAAAAATACT GAACAGGTTT TACCATTAAC TTCTAGCAGT
    GGCAACGGCA CCCAACTATT AAATGCCAGT CAGAATCACT CTGAAGAATT TCTTGCACTT
    GATAAATCAC ATACCCAAGT TTCCAATTCT TCGTTTTACC CTCTGGGAGT AAAATATCCT
    ATATCTAAAG ACACAGAATC ACATATTCTG GAATCTGAAG ATTTGGGAAG GACTAGAAGT
    TTACCTCATG ACAATACTGA CATAGAAACT GCCAGAGAGA GCAAACAGTA CTGGAATACT
    CCTAAAAATT CTTCAGAACT TTCTGGATTA GAAAATAGCA TTTCCCTATT AAAATCATTG
    TCTGAACACA GTACTGCTTT AGAAGGCTTG GAAATATTGA AAAAGAAACA TACCTCCTTT
    AAGCAACAAG GAACTCTGCA GACGTATGAT CCAGAAAGCA GTCCTGAAGC TAGGAAACCA
    TTGACTACAA TAGTTTCACA AGCTATACCA AAGTCTCATC CGAACCAGGA TCCTCAACAG
    ATGATAAAAG AAAATGAAGT TAAACCAAGT GAATCCCTCA CCAAGTTTTT TCCCTTGGGC
    AGTGAAGAAG AGACTGACTC CCGAGCTCTC ATTCCTAAAC AAATGGAGAG AAAAACCTGT
    GACCCTAAGT TGCCAGATGA AGAAAACTTG ATAAAAGGAG ATTCCAGCTT TAACACTGAA
    GACAATTTTC AAGTGATAGC TGAGCCTAAT AAGAATTCAG TTAAAAATGA TCTAATGAAA
    AAGTCTCTGG AGAATAAGAA CCTTCAAAGG CTTTCATTAG AGATAAGTGA CTGTGAGCTG
    TGCTTCCCAA GTGGGGAGAA AGTAAAATCT GCATATCTTC CCCAAAGGCA AATTCACATA
    CCAGCTGTTT TTCAGTCTCC TGCTCATTAT AAACAGATTT TTACATCTTG TCTCATAGAA
    CATCTAAATA TATTGCTGTT TGGGTTGGCA CAAAGGCTGC ACAAAGCTCT TTCAAAAGTT
    GACATATCAT TTTACTCATC AAACGGAGAC AAACGGAAAA ATGTAGAAAA TAATGTGCCA
    TCATGCCATC ATCATCACCC TGCAAAACTT GTCATGGTTA AAAAGGAAGG TCCAAATAAG
    GGTCGTCTCT TTTATGCTTG TGATGGACCC AAAGCTGACC AATGTAAATT TTTCAAGTGG
    CTTGATGAAG TGACCCCAGG ATATTTAACA CAGGAAGAAT CACTACCTAG CATGGTTTTA
    AGTGATATAA AAAGTATTGG CTTGTACTTA AGAAGTCAAA AGATACCCCT CTATGAGGAA
    TGCCAACTTT TGGTGAGAAA AGGATTTGAT TTTCAAAGAA AACGGTATGG TAAACTAAAG
    AAGTTTACAA CTGTAGATCC TGAATTTTAT AACGAACCTA AGAGCAGACT TTATCTTAAG
    CTGAGTCGTA AGGAAAGTTC ATCTGCTTAT AGTAAAGATG ATCTTTGGGT GGTTTCAAAA
    ACCCTAGACT TTGAATTGGA TACTTTTATT GCATGTAGTA CATTCTTTGG ACCGTCATCT
    GTCAATGAGG TAGAGCTACT ACCTTTGAAA GGTTATTTCC CTTCCAACTG GCCCACTAAC
    ACATTGGTCC ATGCATTATT GGTTTGTAAT GCTAGCACGG AGCTGACCAC TTTGAAAAAT
    ATTCAGGACT ACTTTAATCC AGCTACTCTG CCTCTAACAC AGAACCTGTT AACAATGTCT
    TCATCAACTA TAGTGAGCAA CAAGAGAGTC AATAAGAGAA AATTTATCCC ACCAGCCTTC
    TCAAATATCA ATACAAAATT TGAACTGCTC AGTATAGGAG CAACGCTGCA GTTAGCTGGT
    GAGTTAATTC ACACACACAA GTTAAACAAG GATCAAGCTA CAGCCCTAAT TCAGATAGCG
    CAAATGATGG CATCACAAGG AAGTGCTGAA GAAGTGAAGG AACTGGGAAC TCACACCCTC
    CCAATCACGA TCATACATGG TGTTTTTGGA GCAGGAAAAA GTTATTTGCT GGCAGTGGTG
    ATTTTGTTTT TTGTTCAGCT ATTTGAAAAG AGTGAAGCTC TTGCAGTTGG AAATGGAAGA
    CCTTGGAAAC TTCTGATTTC TTCTTCTACT AATGTAGCTG TTGACAGAGT ACTTCTTGGG
    CTTCTTAGTC TTGGGTTTGA AAAGTTTGTC AGAGTTGGGA GTGTCAGGAA GATTGCCAAG
    CCAATTTTAC CTTACAGTTT GCATGCTGGC TCAGAAAATG AAAATGAACA ATTAAAAGAA
    CTGCATACAC TCATGAAGGA AGACCTGACT CCTGTGGAAA GAGTCTATGT GAGAAAAAGT
    ATTGAGCAAC ATAAACTGGG GACCAATAAA ACCCTGCTGA AGCAGGTTCG AGTAGTTGGA
    GTTACCTGTG CAGCCTGCCC ATTTCCATGC ATGAATGACC TTAAATTTCC TGTGGTTGTA
    CTGGATGAGT GTAGCCAAAT AACCGAACCA GCTTCTCTCC TTCCCATTGC AAGGTTTGAG
    TGTGAAAAGC TTATTCTTGT TGGAGATCCC AAACAGCTTC CTCCTACTAT TCAGGGTTCT
    GATGCAGCTC ATGAAAATGG ACTGGAACAA ACTCTTTTTG ATCGACTTTG CTTAATGGGT
    CACAAGCCAG TTCTTTTGCG AACCCAGTAC CGTTGTCACC CTACAATCAG TGCTATTGCT
    AATGATCTGT TTTATGAAGG AAACCTCGTG AATGGAGTTT CAGAAACAGA GAGGAGCCCT
    TTATTGGAAT GGCTTCCAAC CCTGTGTTTC TATAACATTC AAGGGCTGGA ACAGATCGAA
    AGAGATAACA GCTTTCATAA CGTAGCAGAA GCTGCTTTTA CACTCAAGCT GATTCAATCA
    CTGATTGTGA GTGGAATAGC AGGCTCTATG ATTGGGGTGA TAACATTATA CAAATCCCAG
    ATGTACAAGC TTTGTCACTT ACTCACTGCA CTGGACTTTG ACCATCCTGG TAATGTGAAA
    GCTGTGCAGG TGTCCACAGT AGATGCTTTT CAGGGCGCTG AAAAGGAGAT CATCATTCTG
    TCCTGTGTGA GGACAAGACA AGTAGGATTT ATTGATTCAG AACAAAGAAT GAATGTTGCA
    TTGACCAGAG GAAGGAGACA TTTGTTGATT GTGGGAAATT TGGCCTGTTT GAGGAAAAAT
    CGACTATGGG GGCGTGTGAT CCAACACTGT GAAGGCAGGG AAGATGGATT GCAACATGCA
    AGCCAATATG AACCACAGCT CAACCATCTC CTTAAAGATT ATTTTGAAAA ACAAGCAGAA
    GAAAAACAAA AGAAAAAGAA TGAAAAAGAT AAATCCAAAG ATAAATCTCA TTTACAAAAA
    GAAACAGTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020957326.1
    CDS405..5876
    Translation

    Target ORF information:

    RefSeq Version XM_021101667.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa zinc finger GRF-type containing 1 (ZGRF1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021101667.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGAAAGCC AGGAATTTAT TGTCCTATAT ACTCATCAAA AGATGAAGAA GTCAAAAGTA 
    TGGCAAGATG GAATTCTGAA GATCACTCAC GTAGGAAACA AAGCCATTTT ATATGATGAC
    AAAGGACAGT GTTTGGAGAG TTTATTTCTT AAGTGCCTTG AGGTGAAACC TGGAGATGAT
    TTAGAAAGTG ATCGATACTT AATCACAGTT GAGGAGGTTA AAGTTGCTGG AAGCATAGCT
    GTTAAACAAG ATATCATTAA AGGAGCACCT CCATTAAATT CAAAGAGCTT TATACCTTCT
    GGCCGGTCCC TTGGGTGTCA ACCTGCTGGT TTAAAAAGGA AATTTACTGG TTTTCAAGGA
    CCACGGCAAG TGCCAAAGAA AATGGCTATT ACAGAAAATA GTGAATCAGC TGCTTCACTT
    GAGGCTAAGG AAGCTGGTCC TGTTTTTCTT TCTCCAGTCT ACAGCACACC TCCTTTGTTT
    CCTACTGTTG GCAAAAAAGA TATAAATTAT ATGCCAACAG ACCCTGACAA CCAGGACAGA
    GAGAGAAATG GCATAGCTTT TTCTTCGGTT GTCTCTACTC CATCCTTCAA GATTAACCCA
    GAAATGCTAT GTGAAGAAAA TTATTTTTGC TCTACTGTCA ATTCTGTAAA TAAGCATTCA
    GGTTCTTTAC TGACCGATGA GCTTATGAAA AGAGATTGTT TGGTATCTCA CAATTCAGGA
    GTTTCACAGA ACATTAGAAG TAAAGCCCAG ATATTGGCTC TTCTGAAATC CAAATCCACT
    GGTACATCTA AAGAACCAAA TTCTGAGATT ACAGGACATT TCCCTCTGAT ACAACCACAA
    GGAAGTTTAA AAATTCCTGC TAAACTTGAG TGCTTAATTG AACAGGAAGA ACACACTCAG
    GTGAAGAGCA CAGAAAGTTT ATACTGCCAA CATCAATCAA AAAATACCAT GAGAAATAAA
    AGCCGGTGGG CCATGTATTT ATCCTCACAG AGTTCACCTA CACATTCTTC TACAGTAGAT
    GGAAATAATA CAGGAAAAAA TCCTGAGGCC GAGGGAGGTA ATATAAATTT AAATTTGAAA
    GACGCTTTGG TGCAAAAAAG CATGCGGTTT TTAGAAACAT GTACTGAAGA GGGAAAAAAG
    TATAATGAAG ACAAGCCAGT AGTTGATAAT GATCAATCCT GGAATCAAAA AGTAAAATTA
    GAATTTCCTT CATTCTGTGA AAGCAGTGCC TTACCAGTTA CTTGTGACAA CGTAGAAAAC
    GACAGCTTAT TATCAGAAAA TGACAGCTTA TTATCAGAAA ATGACATTCA AGAAAATAAT
    AAAAGACTTT TTAATCAAAA TGACCAGATG GGCATAAAAG GATCAATTCT CATTACAGAA
    AAAGCTCAGG AGATAACCAT GTATGGAACC CCAGCTAAGG AGTATAAACA TCTGCAGATT
    GAATCTTCAC TAAGTAACGA TTTTAGGGTC TCTAATGGCA TTGCTGACAT GTTTTCTAAA
    AGTAATGCTG ATAATGAAAA TCTGAACAGT ATTCATGAAC CACTTGTGGA GATTACTTTT
    AATCTGAACA ATTTTGAAAC CAGTGACTCT GAGGAGGAAT CACAGGAAAG CAGCAGAATT
    TCCCACGATT CAGAGGGTTG GGTGAAGGAA ACTTTAGTTA AAGACAATTC AAGCTGTGAG
    AATATAAACT GTGCAGAAGT TGGCAGTGAC CATTTGCCTC TCTTAACGTC TAGTGGGGAC
    AGACTTGCAG AGACATTTTC CATGAAAGAG ACTCTGCTAT CACAATTTTG TGATAAAACT
    TCTGTAGGTT TTGACACAGG ACCTTGGAAA GCTGGAAACA CTAGAAAAGA AATAGAGGAG
    GAGTACGGTG ACACCTCAAA CAATCTTGAC TCATCTTTAG AGTGGACTGA AGATGCAAAT
    GTAGGTAGTA ATGAAGGCAC TAATAAATCT ACTCAGGAAG TCACAGTTAA CTATGATTTT
    GCTTCTCTCC CAAATGAAAG TATAAACACA AATTTACATA TCCCTCACTC TTTAAACACA
    GCCACTAATC AGGATCCTGA AAACAATCCA TGGTCACAAC AGGCTCAGCC TCAGCCTTTT
    ATTATGGGAA CTGACTTACA CAAAAATACT GAACAGGTTT TACCATTAAC TTCTAGCAGT
    GGCAACGGCA CCCAACTATT AAATGCCAGT CAGAATCACT CTGAAGAATT TCTTGCACTT
    GATAAATCAC ATACCCAAGT TTCCAATTCT TCGTTTTACC CTCTGGGAGT AAAATATCCT
    ATATCTAAAG ACACAGAATC ACATATTCTG GAATCTGAAG ATTTGGGAAG GACTAGAAGT
    TTACCTCATG ACAATACTGA CATAGAAACT GCCAGAGAGA GCAAACAGTA CTGGAATACT
    CCTAAAAATT CTTCAGAACT TTCTGGATTA GAAAATAGCA TTTCCCTATT AAAATCATTG
    TCTGAACACA GTACTGCTTT AGAAGGCTTG GAAATATTGA AAAAGAAACA TACCTCCTTT
    AAGCAACAAG GAACTCTGCA GACGTATGAT CCAGAAAGCA GTCCTGAAGC TAGGAAACCA
    TTGACTACAA TAGTTTCACA AGCTATACCA AAGTCTCATC CGAACCAGGA TCCTCAACAG
    ATGATAAAAG AAAATGAAGT TAAACCAAGT GAATCCCTCA CCAAGTTTTT TCCCTTGGGC
    AGTGAAGAAG AGACTGACTC CCGAGCTCTC ATTCCTAAAC AAATGGAGAG AAAAACCTGT
    GACCCTAAGT TGCCAGATGA AGAAAACTTG ATAAAAGGAG ATTCCAGCTT TAACACTGAA
    GACAATTTTC AAGTGATAGC TGAGCCTAAT AAGAATTCAG TTAAAAATGA TCTAATGAAA
    AAGTCTCTGG AGAATAAGAA CCTTCAAAGG CTTTCATTAG AGATAAGTGA CTGTGAGCTG
    TGCTTCCCAA GTGGGGAGAA AGTAAAATCT GCATATCTTC CCCAAAGGCA AATTCACATA
    CCAGCTGTTT TTCAGTCTCC TGCTCATTAT AAACAGATTT TTACATCTTG TCTCATAGAA
    CATCTAAATA TATTGCTGTT TGGGTTGGCA CAAAGGCTGC ACAAAGCTCT TTCAAAAGTT
    GACATATCAT TTTACTCATC AAACGGAGAC AAACGGAAAA ATGTAGAAAA TAATGTGCCA
    TCATGCCATC ATCATCACCC TGCAAAACTT GTCATGGTTA AAAAGGAAGG TCCAAATAAG
    GGTCGTCTCT TTTATGCTTG TGATGGACCC AAAGCTGACC AATGTAAATT TTTCAAGTGG
    CTTGATGAAG TGACCCCAGG ATATTTAACA CAGGAAGAAT CACTACCTAG CATGGTTTTA
    AGTGATATAA AAAGTATTGG CTTGTACTTA AGAAGTCAAA AGATACCCCT CTATGAGGAA
    TGCCAACTTT TGGTGAGAAA AGGATTTGAT TTTCAAAGAA AACGGTATGG TAAACTAAAG
    AAGTTTACAA CTGTAGATCC TGAATTTTAT AACGAACCTA AGAGCAGACT TTATCTTAAG
    CTGAGTCGTA AGGAAAGTTC ATCTGCTTAT AGTAAAGATG ATCTTTGGGT GGTTTCAAAA
    ACCCTAGACT TTGAATTGGA TACTTTTATT GCATGTAGTA CATTCTTTGG ACCGTCATCT
    GTCAATGAGG TAGAGCTACT ACCTTTGAAA GGTTATTTCC CTTCCAACTG GCCCACTAAC
    ACATTGGTCC ATGCATTATT GGTTTGTAAT GCTAGCACGG AGCTGACCAC TTTGAAAAAT
    ATTCAGGACT ACTTTAATCC AGCTACTCTG CCTCTAACAC AGAACCTGTT AACAATGTCT
    TCATCAACTA TAGTGAGCAA CAAGAGAGTC AATAAGAGAA AATTTATCCC ACCAGCCTTC
    TCAAATATCA ATACAAAATT TGAACTGCTC AGTATAGGAG CAACGCTGCA GTTAGCTGGT
    GAGTTAATTC ACACACACAA GTTAAACAAG GATCAAGCTA CAGCCCTAAT TCAGATAGCG
    CAAATGATGG CATCACAAGG AAGTGCTGAA GAAGTGAAGG AACTGGGAAC TCACACCCTC
    CCAATCACGA TCATACATGG TGTTTTTGGA GCAGGAAAAA GTTATTTGCT GGCAGTGGTG
    ATTTTGTTTT TTGTTCAGCT ATTTGAAAAG AGTGAAGCTC TTGCAGTTGG AAATGGAAGA
    CCTTGGAAAC TTCTGATTTC TTCTTCTACT AATGTAGCTG TTGACAGAGT ACTTCTTGGG
    CTTCTTAGTC TTGGGTTTGA AAAGTTTGTC AGAGTTGGGA GTGTCAGGAA GATTGCCAAG
    CCAATTTTAC CTTACAGTTT GCATGCTGGC TCAGAAAATG AAAATGAACA ATTAAAAGAA
    CTGCATACAC TCATGAAGGA AGACCTGACT CCTGTGGAAA GAGTCTATGT GAGAAAAAGT
    ATTGAGCAAC ATAAACTGGG GACCAATAAA ACCCTGCTGA AGCAGGTTCG AGTAGTTGGA
    GTTACCTGTG CAGCCTGCCC ATTTCCATGC ATGAATGACC TTAAATTTCC TGTGGTTGTA
    CTGGATGAGT GTAGCCAAAT AACCGAACCA GCTTCTCTCC TTCCCATTGC AAGGTTTGAG
    TGTGAAAAGC TTATTCTTGT TGGAGATCCC AAACAGCTTC CTCCTACTAT TCAGGGTTCT
    GATGCAGCTC ATGAAAATGG ACTGGAACAA ACTCTTTTTG ATCGACTTTG CTTAATGGGT
    CACAAGCCAG TTCTTTTGCG AACCCAGTAC CGTTGTCACC CTACAATCAG TGCTATTGCT
    AATGATCTGT TTTATGAAGG AAACCTCGTG AATGGAGTTT CAGAAACAGA GAGGAGCCCT
    TTATTGGAAT GGCTTCCAAC CCTGTGTTTC TATAACATTC AAGGGCTGGA ACAGATCGAA
    AGAGATAACA GCTTTCATAA CGTAGCAGAA GCTGCTTTTA CACTCAAGCT GATTCAATCA
    CTGATTGTGA GTGGAATAGC AGGCTCTATG ATTGGGGTGA TAACATTATA CAAATCCCAG
    ATGTACAAGC TTTGTCACTT ACTCACTGCA CTGGACTTTG ACCATCCTGG TAATGTGAAA
    GCTGTGCAGG TGTCCACAGT AGATGCTTTT CAGGGCGCTG AAAAGGAGAT CATCATTCTG
    TCCTGTGTGA GGACAAGACA AGTAGGATTT ATTGATTCAG AACAAAGAAT GAATGTTGCA
    TTGACCAGAG GAAGGAGACA TTTGTTGATT GTGGGAAATT TGGCCTGTTT GAGGAAAAAT
    CGACTATGGG GGCGTGTGAT CCAACACTGT GAAGGCAGGG AAGATGGATT GCAACATGCA
    AGCCAATATG AACCACAGCT CAACCATCTC CTTAAAGATT ATTTTGAAAA ACAAGCAGAA
    GAAAAACAAA AGAAAAAGAA TGAAAAAGAT AAATCCAAAG ATAAATCTCA TTTACAAAAA
    GAAACAGTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe224831
    Clone ID Related Accession (Same CDS sequence) XM_021101668.1
    Accession Version XM_021101668.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5328bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product protein ZGRF1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010450.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGAAAGCC AGGAATTTAT TGTCCTATAT ACTCATCAAA AGATGAAGAA GTCAAAAGTA 
    TGGCAAGATG GAATTCTGAA GATCACTCAC GTAGGAAACA AAGCCATTTT ATATGATGAC
    AAAGGACAGT GTTTGGAGAG TTTATTTCTT AAGTGCCTTG AGGTGAAACC TGGAGATGAT
    TTAGAAAGTG ATCGATACTT AATCACAGTT GAGGAGGTTA AAGTTGCTGG AAGCATAGCT
    GTTAAACAAG ATATCATTAA AGGAGCACCT CCATTAAATT CAAAGAGCTT TATACCTTCT
    GGCCGGTCCC TTGGGTGTCA ACCTGCTGGT TTAAAAAGGA AATTTACTGG TTTTCAAGGA
    CCACGGCAAG TGCCAAAGAA AATGGCTATT ACAGAAAATA GTGAATCAGC TGCTTCACTT
    GAGGCTAAGG AAGCTGGTCC TGTTTTTCTT TCTCCAGTCT ACAGCACACC TCCTTTGTTT
    CCTACTGTTG GCAAAAAAGA TATAAATTAT ATGCCAACAG ACCCTGACAA CCAGGACAGA
    GAGAGAAATG GCATAGCTTT TTCTTCGGTT GTCTCTACTC CATCCTTCAA GATTAACCCA
    GAAATGCTAT GTGAAGAAAA TTATTTTTGC TCTACTGTCA ATTCTGTAAA TAAGCATTCA
    GGTTCTTTAC TGACCGATGA GCTTATGAAA AGAGATTGTT TGGTATCTCA CAATTCAGGA
    GTTTCACAGA ACATTAGAAG TAAAGCCCAG ATATTGGCTC TTCTGAAATC CAAATCCACT
    GGTACATCTA AAGAACCAAA TTCTGAGATT ACAGGACATT TCCCTCTGAT ACAACCACAA
    GGAAGTTTAA AAATTCCTGC TAAACTTGAG TGCTTAATTG AACAGGAAGA ACACACTCAG
    GTGAAGAGCA CAGAAAGTTT ATACTGCCAA CATCAATCAA AAAATACCAT GAGAAATAAA
    AGCCGGTGGG CCATGTATTT ATCCTCACAG AGTTCACCTA CACATTCTTC TACAGTAGAT
    GGAAATAATA CAGGAAAAAA TCCTGAGGCC GAGGGAGGTA ATATAAATTT AAATTTGAAA
    GACGCTTTGG TGCAAAAAAG CATGCGGTTT TTAGAAACAT GTACTGAAGA GGGAAAAAAG
    TATAATGAAG ACAAGCCAGT AGTTGATAAT GATCAATCCT GGAATCAAAA AGTAAAATTA
    GAATTTCCTT CATTCTGTGA AAGCAGTGCC TTACCAGTTA CTTGTGACAA CGTAGAAAAC
    GACAGCTTAT TATCAGAAAA TGACAGCTTA TTATCAGAAA ATGACATTCA AGAAAATAAT
    AAAAGACTTT TTAATCAAAA TGACCAGATG GGCATAAAAG GATCAATTCT CATTACAGAA
    AAAGCTCAGG AGATAACCAT GTATGGAACC CCAGCTAAGG AGTATAAACA TCTGCAGATT
    GAATCTTCAC TAAGTAACGA TTTTAGGGTC TCTAATGGCA TTGCTGACAT GTTTTCTAAA
    AGTAATGCTG ATAATGAAAA TCTGAACAGT ATTCATGAAC CACTTGTGGA GATTACTTTT
    AATCTGAACA ATTTTGAAAC CAGTGACTCT GAGGAGGAAT CACAGGAAAG CAGCAGAATT
    TCCCACGATT CAGAGGGTTG GGTGAAGGAA ACTTTAGTTA AAGACAATTC AAGCTGTGAG
    AATATAAACT GTGCAGAAGT TGGCAGTGAC CATTTGCCTC TCTTAACGTC TAGTGGGGAC
    AGACTTGCAG AGACATTTTC CATGAAAGAG ACTCTGCTAT CACAATTTTG TGATAAAACT
    TCTGTAGGTT TTGACACAGG ACCTTGGAAA GCTGGAAACA CTAGAAAAGA AATAGAGGAG
    GAGTACGGTG ACACCTCAAA CAATCTTGAC TCATCTTTAG AGTGGACTGA AGATGCAAAT
    GTAGGTAGTA ATGAAGGCAC TAATAAATCT ACTCAGGAAG TCACAGTTAA CTATGATTTT
    GCTTCTCTCC CAAATGAAAG TATAAACACA AATTTACATA TCCCTCACTC TTTAAACACA
    GCCACTAATC AGGATCCTGA AAACAATCCA TGGTCACAAC AGGCTCAGCC TCAGCCTTTT
    ATTATGGGAA CTGACTTACA CAAAAATACT GAACAGGTTT TACCATTAAC TTCTAGCAGT
    GGCAACGGCA CCCAACTATT AAATGCCAGT CAGAATCACT CTGAAGAATT TCTTGCACTT
    GATAAATCAC ATACCCAAGT TTCCAATTCT TCGTTTTACC CTCTGGGAGT AAAATATCCT
    ATATCTAAAG ACACAGAATC ACATATTCTG GAATCTGAAG ATTTGGGAAG GACTAGAAGT
    TTACCTCATG ACAATACTGA CATAGAAACT GCCAGAGAGA GCAAACAGTA CTGGAATACT
    CCTAAAAATT CTTCAGAACT TTCTGGATTA GAAAATAGCA TTTCCCTATT AAAATCATTG
    TCTGAACACA GTACTGCTTT AGAAGGCTTG GAAATATTGA AAAAGAAACA TACCTCCTTT
    AAGCAACAAG GAACTCTGCA GACGTATGAT CCAGAAAGCA GTCCTGAAGC TAGGAAACCA
    TTGACTACAA TAGTTTCACA AGCTATACCA AAGTCTCATC CGAACCAGGA TCCTCAACAG
    ATGATAAAAG AAAATGAAGT TAAACCAAGT GAATCCCTCA CCAAGTTTTT TCCCTTGGGC
    AGTGAAGAAG AGACTGACTC CCGAGCTCTC ATTCCTAAAC AAATGGAGAG AAAAACCTGT
    GACCCTAAGT TGCCAGATGA AGAAAACTTG ATAAAAGGAG ATTCCAGCTT TAACACTGAA
    GACAATTTTC AAGTGATAGC TGAGCCTAAT AAGAATTCAG TTAAAAATGA TCTAATGAAA
    AAGTCTCTGG AGAATAAGAA CCTTCAAAGG CTTTCATTAG AGATAAGTGA CTGTGAGCTG
    TGCTTCCCAA GTGGGGAGAA AGTAAAATCT GCATATCTTC CCCAAAGGCA AATTCACATA
    CCAGCTGTTT TTCAGTCTCC TGCTCATTAT AAACAGATTT TTACATCTTG TCTCATAGAA
    CATCTAAATA TATTGCTGTT TGGGTTGGCA CAAAGGCTGC ACAAAGCTCT TTCAAAAGTT
    GACATATCAT TTTACTCATC AAACGGAGAC AAACGGAAAA ATGTAGAAAA TAATGTGCCA
    TCATGCCATC ATCATCACCC TGCAAAACTT GTCATGGTTA AAAAGGAAGG TCCAAATAAG
    GGTCGTCTCT TTTATGCTTG TGATGGACCC AAAGCTGACC AATGTAAATT TTTCAAGTGG
    CTTGATGAAG TGACCCCAGG ATATTTAACA CAGGAAGAAT CACTACCTAG CATGGTTTTA
    AGTGATATAA AAAGTATTGG CTTGTACTTA AGAAGTCAAA AGATACCCCT CTATGAGGAA
    TGCCAACTTT TGGTGAGAAA AGGATTTGAT TTTCAAAGAA AACGGTATGG TAAACTAAAG
    AAGTTTACAA CTGTAGATCC TGAATTTTAT AACGAACCTA AGAGCAGACT TTATCTTAAG
    CTGAGTCGTA AGGAAAGTTC ATCTGCTTAT AGTAAAGCAT TGGTCCATGC ATTATTGGTT
    TGTAATGCTA GCACGGAGCT GACCACTTTG AAAAATATTC AGGACTACTT TAATCCAGCT
    ACTCTGCCTC TAACACAGAA CCTGTTAACA ATGTCTTCAT CAACTATAGT GAGCAACAAG
    AGAGTCAATA AGAGAAAATT TATCCCACCA GCCTTCTCAA ATATCAATAC AAAATTTGAA
    CTGCTCAGTA TAGGAGCAAC GCTGCAGTTA GCTGGTGAGT TAATTCACAC ACACAAGTTA
    AACAAGGATC AAGCTACAGC CCTAATTCAG ATAGCGCAAA TGATGGCATC ACAAGGAAGT
    GCTGAAGAAG TGAAGGAACT GGGAACTCAC ACCCTCCCAA TCACGATCAT ACATGGTGTT
    TTTGGAGCAG GAAAAAGTTA TTTGCTGGCA GTGGTGATTT TGTTTTTTGT TCAGCTATTT
    GAAAAGAGTG AAGCTCTTGC AGTTGGAAAT GGAAGACCTT GGAAACTTCT GATTTCTTCT
    TCTACTAATG TAGCTGTTGA CAGAGTACTT CTTGGGCTTC TTAGTCTTGG GTTTGAAAAG
    TTTGTCAGAG TTGGGAGTGT CAGGAAGATT GCCAAGCCAA TTTTACCTTA CAGTTTGCAT
    GCTGGCTCAG AAAATGAAAA TGAACAATTA AAAGAACTGC ATACACTCAT GAAGGAAGAC
    CTGACTCCTG TGGAAAGAGT CTATGTGAGA AAAAGTATTG AGCAACATAA ACTGGGGACC
    AATAAAACCC TGCTGAAGCA GGTTCGAGTA GTTGGAGTTA CCTGTGCAGC CTGCCCATTT
    CCATGCATGA ATGACCTTAA ATTTCCTGTG GTTGTACTGG ATGAGTGTAG CCAAATAACC
    GAACCAGCTT CTCTCCTTCC CATTGCAAGG TTTGAGTGTG AAAAGCTTAT TCTTGTTGGA
    GATCCCAAAC AGCTTCCTCC TACTATTCAG GGTTCTGATG CAGCTCATGA AAATGGACTG
    GAACAAACTC TTTTTGATCG ACTTTGCTTA ATGGGTCACA AGCCAGTTCT TTTGCGAACC
    CAGTACCGTT GTCACCCTAC AATCAGTGCT ATTGCTAATG ATCTGTTTTA TGAAGGAAAC
    CTCGTGAATG GAGTTTCAGA AACAGAGAGG AGCCCTTTAT TGGAATGGCT TCCAACCCTG
    TGTTTCTATA ACATTCAAGG GCTGGAACAG ATCGAAAGAG ATAACAGCTT TCATAACGTA
    GCAGAAGCTG CTTTTACACT CAAGCTGATT CAATCACTGA TTGTGAGTGG AATAGCAGGC
    TCTATGATTG GGGTGATAAC ATTATACAAA TCCCAGATGT ACAAGCTTTG TCACTTACTC
    ACTGCACTGG ACTTTGACCA TCCTGGTAAT GTGAAAGCTG TGCAGGTGTC CACAGTAGAT
    GCTTTTCAGG GCGCTGAAAA GGAGATCATC ATTCTGTCCT GTGTGAGGAC AAGACAAGTA
    GGATTTATTG ATTCAGAACA AAGAATGAAT GTTGCATTGA CCAGAGGAAG GAGACATTTG
    TTGATTGTGG GAAATTTGGC CTGTTTGAGG AAAAATCGAC TATGGGGGCG TGTGATCCAA
    CACTGTGAAG GCAGGGAAGA TGGATTGCAA CATGCAAGCC AATATGAACC ACAGCTCAAC
    CATCTCCTTA AAGATTATTT TGAAAAACAA GCAGAAGAAA AACAAAAGAA AAAGAATGAA
    AAAGATAAAT CCAAAGATAA ATCTCATTTA CAAAAAGAAA CAGTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020957327.1
    CDS405..5732
    Translation

    Target ORF information:

    RefSeq Version XM_021101668.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa zinc finger GRF-type containing 1 (ZGRF1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021101668.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGAAAGCC AGGAATTTAT TGTCCTATAT ACTCATCAAA AGATGAAGAA GTCAAAAGTA 
    TGGCAAGATG GAATTCTGAA GATCACTCAC GTAGGAAACA AAGCCATTTT ATATGATGAC
    AAAGGACAGT GTTTGGAGAG TTTATTTCTT AAGTGCCTTG AGGTGAAACC TGGAGATGAT
    TTAGAAAGTG ATCGATACTT AATCACAGTT GAGGAGGTTA AAGTTGCTGG AAGCATAGCT
    GTTAAACAAG ATATCATTAA AGGAGCACCT CCATTAAATT CAAAGAGCTT TATACCTTCT
    GGCCGGTCCC TTGGGTGTCA ACCTGCTGGT TTAAAAAGGA AATTTACTGG TTTTCAAGGA
    CCACGGCAAG TGCCAAAGAA AATGGCTATT ACAGAAAATA GTGAATCAGC TGCTTCACTT
    GAGGCTAAGG AAGCTGGTCC TGTTTTTCTT TCTCCAGTCT ACAGCACACC TCCTTTGTTT
    CCTACTGTTG GCAAAAAAGA TATAAATTAT ATGCCAACAG ACCCTGACAA CCAGGACAGA
    GAGAGAAATG GCATAGCTTT TTCTTCGGTT GTCTCTACTC CATCCTTCAA GATTAACCCA
    GAAATGCTAT GTGAAGAAAA TTATTTTTGC TCTACTGTCA ATTCTGTAAA TAAGCATTCA
    GGTTCTTTAC TGACCGATGA GCTTATGAAA AGAGATTGTT TGGTATCTCA CAATTCAGGA
    GTTTCACAGA ACATTAGAAG TAAAGCCCAG ATATTGGCTC TTCTGAAATC CAAATCCACT
    GGTACATCTA AAGAACCAAA TTCTGAGATT ACAGGACATT TCCCTCTGAT ACAACCACAA
    GGAAGTTTAA AAATTCCTGC TAAACTTGAG TGCTTAATTG AACAGGAAGA ACACACTCAG
    GTGAAGAGCA CAGAAAGTTT ATACTGCCAA CATCAATCAA AAAATACCAT GAGAAATAAA
    AGCCGGTGGG CCATGTATTT ATCCTCACAG AGTTCACCTA CACATTCTTC TACAGTAGAT
    GGAAATAATA CAGGAAAAAA TCCTGAGGCC GAGGGAGGTA ATATAAATTT AAATTTGAAA
    GACGCTTTGG TGCAAAAAAG CATGCGGTTT TTAGAAACAT GTACTGAAGA GGGAAAAAAG
    TATAATGAAG ACAAGCCAGT AGTTGATAAT GATCAATCCT GGAATCAAAA AGTAAAATTA
    GAATTTCCTT CATTCTGTGA AAGCAGTGCC TTACCAGTTA CTTGTGACAA CGTAGAAAAC
    GACAGCTTAT TATCAGAAAA TGACAGCTTA TTATCAGAAA ATGACATTCA AGAAAATAAT
    AAAAGACTTT TTAATCAAAA TGACCAGATG GGCATAAAAG GATCAATTCT CATTACAGAA
    AAAGCTCAGG AGATAACCAT GTATGGAACC CCAGCTAAGG AGTATAAACA TCTGCAGATT
    GAATCTTCAC TAAGTAACGA TTTTAGGGTC TCTAATGGCA TTGCTGACAT GTTTTCTAAA
    AGTAATGCTG ATAATGAAAA TCTGAACAGT ATTCATGAAC CACTTGTGGA GATTACTTTT
    AATCTGAACA ATTTTGAAAC CAGTGACTCT GAGGAGGAAT CACAGGAAAG CAGCAGAATT
    TCCCACGATT CAGAGGGTTG GGTGAAGGAA ACTTTAGTTA AAGACAATTC AAGCTGTGAG
    AATATAAACT GTGCAGAAGT TGGCAGTGAC CATTTGCCTC TCTTAACGTC TAGTGGGGAC
    AGACTTGCAG AGACATTTTC CATGAAAGAG ACTCTGCTAT CACAATTTTG TGATAAAACT
    TCTGTAGGTT TTGACACAGG ACCTTGGAAA GCTGGAAACA CTAGAAAAGA AATAGAGGAG
    GAGTACGGTG ACACCTCAAA CAATCTTGAC TCATCTTTAG AGTGGACTGA AGATGCAAAT
    GTAGGTAGTA ATGAAGGCAC TAATAAATCT ACTCAGGAAG TCACAGTTAA CTATGATTTT
    GCTTCTCTCC CAAATGAAAG TATAAACACA AATTTACATA TCCCTCACTC TTTAAACACA
    GCCACTAATC AGGATCCTGA AAACAATCCA TGGTCACAAC AGGCTCAGCC TCAGCCTTTT
    ATTATGGGAA CTGACTTACA CAAAAATACT GAACAGGTTT TACCATTAAC TTCTAGCAGT
    GGCAACGGCA CCCAACTATT AAATGCCAGT CAGAATCACT CTGAAGAATT TCTTGCACTT
    GATAAATCAC ATACCCAAGT TTCCAATTCT TCGTTTTACC CTCTGGGAGT AAAATATCCT
    ATATCTAAAG ACACAGAATC ACATATTCTG GAATCTGAAG ATTTGGGAAG GACTAGAAGT
    TTACCTCATG ACAATACTGA CATAGAAACT GCCAGAGAGA GCAAACAGTA CTGGAATACT
    CCTAAAAATT CTTCAGAACT TTCTGGATTA GAAAATAGCA TTTCCCTATT AAAATCATTG
    TCTGAACACA GTACTGCTTT AGAAGGCTTG GAAATATTGA AAAAGAAACA TACCTCCTTT
    AAGCAACAAG GAACTCTGCA GACGTATGAT CCAGAAAGCA GTCCTGAAGC TAGGAAACCA
    TTGACTACAA TAGTTTCACA AGCTATACCA AAGTCTCATC CGAACCAGGA TCCTCAACAG
    ATGATAAAAG AAAATGAAGT TAAACCAAGT GAATCCCTCA CCAAGTTTTT TCCCTTGGGC
    AGTGAAGAAG AGACTGACTC CCGAGCTCTC ATTCCTAAAC AAATGGAGAG AAAAACCTGT
    GACCCTAAGT TGCCAGATGA AGAAAACTTG ATAAAAGGAG ATTCCAGCTT TAACACTGAA
    GACAATTTTC AAGTGATAGC TGAGCCTAAT AAGAATTCAG TTAAAAATGA TCTAATGAAA
    AAGTCTCTGG AGAATAAGAA CCTTCAAAGG CTTTCATTAG AGATAAGTGA CTGTGAGCTG
    TGCTTCCCAA GTGGGGAGAA AGTAAAATCT GCATATCTTC CCCAAAGGCA AATTCACATA
    CCAGCTGTTT TTCAGTCTCC TGCTCATTAT AAACAGATTT TTACATCTTG TCTCATAGAA
    CATCTAAATA TATTGCTGTT TGGGTTGGCA CAAAGGCTGC ACAAAGCTCT TTCAAAAGTT
    GACATATCAT TTTACTCATC AAACGGAGAC AAACGGAAAA ATGTAGAAAA TAATGTGCCA
    TCATGCCATC ATCATCACCC TGCAAAACTT GTCATGGTTA AAAAGGAAGG TCCAAATAAG
    GGTCGTCTCT TTTATGCTTG TGATGGACCC AAAGCTGACC AATGTAAATT TTTCAAGTGG
    CTTGATGAAG TGACCCCAGG ATATTTAACA CAGGAAGAAT CACTACCTAG CATGGTTTTA
    AGTGATATAA AAAGTATTGG CTTGTACTTA AGAAGTCAAA AGATACCCCT CTATGAGGAA
    TGCCAACTTT TGGTGAGAAA AGGATTTGAT TTTCAAAGAA AACGGTATGG TAAACTAAAG
    AAGTTTACAA CTGTAGATCC TGAATTTTAT AACGAACCTA AGAGCAGACT TTATCTTAAG
    CTGAGTCGTA AGGAAAGTTC ATCTGCTTAT AGTAAAGCAT TGGTCCATGC ATTATTGGTT
    TGTAATGCTA GCACGGAGCT GACCACTTTG AAAAATATTC AGGACTACTT TAATCCAGCT
    ACTCTGCCTC TAACACAGAA CCTGTTAACA ATGTCTTCAT CAACTATAGT GAGCAACAAG
    AGAGTCAATA AGAGAAAATT TATCCCACCA GCCTTCTCAA ATATCAATAC AAAATTTGAA
    CTGCTCAGTA TAGGAGCAAC GCTGCAGTTA GCTGGTGAGT TAATTCACAC ACACAAGTTA
    AACAAGGATC AAGCTACAGC CCTAATTCAG ATAGCGCAAA TGATGGCATC ACAAGGAAGT
    GCTGAAGAAG TGAAGGAACT GGGAACTCAC ACCCTCCCAA TCACGATCAT ACATGGTGTT
    TTTGGAGCAG GAAAAAGTTA TTTGCTGGCA GTGGTGATTT TGTTTTTTGT TCAGCTATTT
    GAAAAGAGTG AAGCTCTTGC AGTTGGAAAT GGAAGACCTT GGAAACTTCT GATTTCTTCT
    TCTACTAATG TAGCTGTTGA CAGAGTACTT CTTGGGCTTC TTAGTCTTGG GTTTGAAAAG
    TTTGTCAGAG TTGGGAGTGT CAGGAAGATT GCCAAGCCAA TTTTACCTTA CAGTTTGCAT
    GCTGGCTCAG AAAATGAAAA TGAACAATTA AAAGAACTGC ATACACTCAT GAAGGAAGAC
    CTGACTCCTG TGGAAAGAGT CTATGTGAGA AAAAGTATTG AGCAACATAA ACTGGGGACC
    AATAAAACCC TGCTGAAGCA GGTTCGAGTA GTTGGAGTTA CCTGTGCAGC CTGCCCATTT
    CCATGCATGA ATGACCTTAA ATTTCCTGTG GTTGTACTGG ATGAGTGTAG CCAAATAACC
    GAACCAGCTT CTCTCCTTCC CATTGCAAGG TTTGAGTGTG AAAAGCTTAT TCTTGTTGGA
    GATCCCAAAC AGCTTCCTCC TACTATTCAG GGTTCTGATG CAGCTCATGA AAATGGACTG
    GAACAAACTC TTTTTGATCG ACTTTGCTTA ATGGGTCACA AGCCAGTTCT TTTGCGAACC
    CAGTACCGTT GTCACCCTAC AATCAGTGCT ATTGCTAATG ATCTGTTTTA TGAAGGAAAC
    CTCGTGAATG GAGTTTCAGA AACAGAGAGG AGCCCTTTAT TGGAATGGCT TCCAACCCTG
    TGTTTCTATA ACATTCAAGG GCTGGAACAG ATCGAAAGAG ATAACAGCTT TCATAACGTA
    GCAGAAGCTG CTTTTACACT CAAGCTGATT CAATCACTGA TTGTGAGTGG AATAGCAGGC
    TCTATGATTG GGGTGATAAC ATTATACAAA TCCCAGATGT ACAAGCTTTG TCACTTACTC
    ACTGCACTGG ACTTTGACCA TCCTGGTAAT GTGAAAGCTG TGCAGGTGTC CACAGTAGAT
    GCTTTTCAGG GCGCTGAAAA GGAGATCATC ATTCTGTCCT GTGTGAGGAC AAGACAAGTA
    GGATTTATTG ATTCAGAACA AAGAATGAAT GTTGCATTGA CCAGAGGAAG GAGACATTTG
    TTGATTGTGG GAAATTTGGC CTGTTTGAGG AAAAATCGAC TATGGGGGCG TGTGATCCAA
    CACTGTGAAG GCAGGGAAGA TGGATTGCAA CATGCAAGCC AATATGAACC ACAGCTCAAC
    CATCTCCTTA AAGATTATTT TGAAAAACAA GCAGAAGAAA AACAAAAGAA AAAGAATGAA
    AAAGATAAAT CCAAAGATAA ATCTCATTTA CAAAAAGAAA CAGTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe224826
    Clone ID Related Accession (Same CDS sequence) XM_021101661.1 , XM_021101663.1 , XM_021101662.1
    Accession Version XM_021101661.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6255bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product protein ZGRF1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010450.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGAAAGCC AGGAATTTAT TGTCCTATAT ACTCATCAAA AGATGAAGAA GTCAAAAGTA 
    TGGCAAGATG GAATTCTGAA GATCACTCAC GTAGGAAACA AAGCCATTTT ATATGATGAC
    AAAGGACAGT GTTTGGAGAG TTTATTTCTT AAGTGCCTTG AGGTGAAACC TGGAGATGAT
    TTAGAAAGTG ATCGATACTT AATCACAGTT GAGGAGGTTA AAGTTGCTGG AAGCATAGCT
    GTTAAACAAG ATATCATTAA AGGAGCACCT CCATTAAATT CAAAGAGCTT TATACCTTCT
    GGCCGGTCCC TTGGGTGTCA ACCTGCTGGT TTAAAAAGGA AATTTACTGG TTTTCAAGGA
    CCACGGCAAG TGCCAAAGAA AATGGCTATT ACAGAAAATA GTGAATCAGC TGCTTCACTT
    GAGGCTAAGG AAGCTGGTCC TGTTTTTCTT TCTCCAGTCT ACAGCACACC TCCTTTGTTT
    CCTACTGTTG GCAAAAAAGA TATAAATTAT ATGCCAACAG ACCCTGACAA CCAGGACAGA
    GAGAGAAATG GCATAGCTTT TTCTTCGGTT GTCTCTACTC CATCCTTCAA GATTAACCCA
    GAAATGCTAT GTGAAGAAAA TTATTTTTGC TCTACTGTCA ATTCTGTAAA TAAGCATTCA
    GGTTCTTTAC TGACCGATGA GCTTATGAAA AGAGATTGTT TGGTATCTCA CAATTCAGGA
    GTTTCACAGA ACATTAGAAG TAAAGCCCAG ATATTGGCTC TTCTGAAATC CAAATCCACT
    GGTACATCTA AAGAACCAAA TTCTGAGATT ACAGGACATT TCCCTCTGAT ACAACCACAA
    GGAAGTTTAA AAATTCCTGC TAAACTTGAG TGCTTAATTG AACAGGAAGA ACACACTCAG
    GTGAAGAGCA CAGAAAGTTT ATACTGCCAA CATCAATCAA AAAATACCAT GAGAAATAAA
    AGCCGGTGGG CCATGTATTT ATCCTCACAG AGTTCACCTA CACATTCTTC TACAGTAGAT
    GGAAATAATA CAGGAAAAAA TCCTGAGGCC GAGGGAGGTA ATATAAATTT AAATTTGAAA
    GACGCTTTGG TGCAAAAAAG CATGCGGTTT TTAGAAACAT GTACTGAAGA GGGAAAAAAG
    TATAATGAAG ACAAGCCAGT AGTTGATAAT GATCAATCCT GGAATCAAAA AGTAAAATTA
    GAATTTCCTT CATTCTGTGA AAGCAGTGCC TTACCAGTTA CTTGTGACAA CGTAGAAAAC
    GACAGCTTAT TATCAGAAAA TGACAGCTTA TTATCAGAAA ATGACATTCA AGAAAATAAT
    AAAAGACTTT TTAATCAAAA TGACCAGATG GGCATAAAAG GATCAATTCT CATTACAGAA
    AAAGCTCAGG AGATAACCAT GTATGGAACC CCAGCTAAGG AGTATAAACA TCTGCAGATT
    GAATCTTCAC TAAGTAACGA TTTTAGGGTC TCTAATGGCA TTGCTGACAT GTTTTCTAAA
    AGTAATGCTG ATAATGAAAA TCTGAACAGT ATTCATGAAC CACTTGTGGA GATTACTTTT
    AATCTGAACA ATTTTGAAAC CAGTGACTCT GAGGAGGAAT CACAGGAAAG CAGCAGAATT
    TCCCACGATT CAGAGGGTTG GGTGAAGGAA ACTTTAGTTA AAGACAATTC AAGCTGTGAG
    AATATAAACT GTGCAGAAGT TGGCAGTGAC CATTTGCCTC TCTTAACGTC TAGTGGGGAC
    AGACTTGCAG AGACATTTTC CATGAAAGAG ACTCTGCTAT CACAATTTTG TGATAAAACT
    TCTGTAGGTT TTGACACAGG ACCTTGGAAA GCTGGAAACA CTAGAAAAGA AATAGAGGAG
    GAGTACGGTG ACACCTCAAA CAATCTTGAC TCATCTTTAG AGTGGACTGA AGATGCAAAT
    GTAGGTAGTA ATGAAGGCAC TAATAAATCT ACTCAGGAAG TCACAGTTAA CTATGATTTT
    GCTTCTCTCC CAAATGAAAG TATAAACACA AATTTACATA TCCCTCACTC TTTAAACACA
    GCCACTAATC AGGATCCTGA AAACAATCCA TGGTCACAAC AGGCTCAGCC TCAGCCTTTT
    ATTATGGGAA CTGACTTACA CAAAAATACT GAACAGGTTT TACCATTAAC TTCTAGCAGT
    GGCAACGGCA CCCAACTATT AAATGCCAGT CAGAATCACT CTGAAGAATT TCTTGCACTT
    GATAAATCAC ATACCCAAGT TTCCAATTCT TCGTTTTACC CTCTGGGAGT AAAATATCCT
    ATATCTAAAG ACACAGAATC ACATATTCTG GAATCTGAAG ATTTGGGAAG GACTAGAAGT
    TTACCTCATG ACAATACTGA CATAGAAACT GCCAGAGAGA GCAAACAGTA CTGGAATACT
    CCTAAAAATT CTTCAGAACT TTCTGGATTA GAAAATAGCA TTTCCCTATT AAAATCATTG
    TCTGAACACA GTACTGCTTT AGAAGGCTTG GAAATATTGA AAAAGAAACA TACCTCCTTT
    AAGCAACAAG GAACTCTGCA GACGTATGAT CCAGAAAGCA GTCCTGAAGC TAGGAAACCA
    TTGACTACAA TAGTTTCACA AGCTATACCA AAGTCTCATC CGAACCAGGA TCCTCAACAG
    ATGATAAAAG AAAATGAAGT TAAACCAAGT GAATCCCTCA CCAAGTTTTT TCCCTTGGGC
    AGTGAAGAAG AGACTGACTC CCGAGCTCTC ATTCCTAAAC AAATGGAGAG AAAAACCTGT
    GACCCTAAGC CTGTTGAGTT TCAAGGGCAC CAAGTGAAAG GATCAGCTGC TAGTGCTGTG
    ATGGTCAGAG GACACAGCTC CCAGTTTGAA TGCAGTCAAT TTCCAGATAG CGTGTATGAA
    AACTGCATGA CAAATACTTG TTTTTTGACA CCTAAGTTGC CTAGCACTTG TATGCAGATT
    GACTTCTTGC AGTTGCCAGA TGAAGAAAAC TTGATAAAAG GAGATTCCAG CTTTAACACT
    GAAGACAATT TTCAAGTGAT AGCTGAGCCT AATAAGAATT CAGTTAAAAA TGATCTAATG
    AAAAAGTCTC TGGAGAATAA GAACCTTCAA AGGCTTTCAT TAGTAAGTAG AATCCGAGTT
    CCACTTATTA CTTTGCCAGC TACCAGTGGG TCACCTGACC TAGATTCTTG TTCGTGTGTG
    ATCGACTGTG ACAGGCAAGA GTCTTCTGAT TCTCCCATAG TCAACTTGTA TGAAGAGTCA
    GAGCTTCCTT CTTTCAGCTT TGGATCTGAG GACCAAAGTG AAACTTCTTT CTCTAAAGAA
    TCTACTGAAA TGACTCCAAG GGATATTTCA CTTCTCGATA ATACATCTAC TCAAAGCAAG
    TGGCTGAAAT ATCAAAACAT AATCCAATGC AAGTTGACTA CACCAAACAG ACTTGATCAG
    AAAGTAACAG ATGGCTTCTT TGCTGAGGCT GTTTCCAGGA TGCATTTTAG TGACACAGGT
    GAAAGACAGA GTGATGCTGT GAATGAAAGC TTAGACTCTG AGCATTTGCA AATGATAAAA
    TGCATGCTTC AACAACAGCG GCAGGATTTT AGCAGTCAAG ATTTGGTTTC CAGACAGAAA
    GCACTCTCTT TGAATTTAAA GCACACTTCT AAGACTGAGG AAATTAAAAA TGTGTTAGGA
    GAGTCTGCCT GCTACACCTG CAGTGTAAGA GGAGATTTAC AGGAGATAAG TGACTGTGAG
    CTGTGCTTCC CAAGTGGGGA GAAAGTAAAA TCTGCATATC TTCCCCAAAG GCAAATTCAC
    ATACCAGCTG TTTTTCAGTC TCCTGCTCAT TATAAACAGA TTTTTACATC TTGTCTCATA
    GAACATCTAA ATATATTGCT GTTTGGGTTG GCACAAAGGC TGCACAAAGC TCTTTCAAAA
    GTTGACATAT CATTTTACTC ATCAAACGGA GACAAACGGA AAAATGTAGA AAATAATGTG
    CCATCATGCC ATCATCATCA CCCTGCAAAA CTTGTCATGG TTAAAAAGGA AGGTCCAAAT
    AAGGGTCGTC TCTTTTATGC TTGTGATGGA CCCAAAGCTG ACCAATGTAA ATTTTTCAAG
    TGGCTTGATG AAGTGACCCC AGGATATTTA ACACAGGAAG AATCACTACC TAGCATGGTT
    TTAAGTGATA TAAAAAGTAT TGGCTTGTAC TTAAGAAGTC AAAAGATACC CCTCTATGAG
    GAATGCCAAC TTTTGGTGAG AAAAGGATTT GATTTTCAAA GAAAACGGTA TGGTAAACTA
    AAGAAGTTTA CAACTGTAGA TCCTGAATTT TATAACGAAC CTAAGAGCAG ACTTTATCTT
    AAGCTGAGTC GTAAGGAAAG TTCATCTGCT TATAGTAAAG ATGATCTTTG GGTGGTTTCA
    AAAACCCTAG ACTTTGAATT GGATACTTTT ATTGCATGTA GTACATTCTT TGGACCGTCA
    TCTGTCAATG AGGTAGAGCT ACTACCTTTG AAAGGTTATT TCCCTTCCAA CTGGCCCACT
    AACACATTGG TCCATGCATT ATTGGTTTGT AATGCTAGCA CGGAGCTGAC CACTTTGAAA
    AATATTCAGG ACTACTTTAA TCCAGCTACT CTGCCTCTAA CACAGAACCT GTTAACAATG
    TCTTCATCAA CTATAGTGAG CAACAAGAGA GTCAATAAGA GAAAATTTAT CCCACCAGCC
    TTCTCAAATA TCAATACAAA ATTTGAACTG CTCAGTATAG GAGCAACGCT GCAGTTAGCT
    GGTGAGTTAA TTCACACACA CAAGTTAAAC AAGGATCAAG CTACAGCCCT AATTCAGATA
    GCGCAAATGA TGGCATCACA AGGAAGTGCT GAAGAAGTGA AGGAACTGGG AACTCACACC
    CTCCCAATCA CGATCATACA TGGTGTTTTT GGAGCAGGAA AAAGTTATTT GCTGGCAGTG
    GTGATTTTGT TTTTTGTTCA GCTATTTGAA AAGAGTGAAG CTCTTGCAGT TGGAAATGGA
    AGACCTTGGA AACTTCTGAT TTCTTCTTCT ACTAATGTAG CTGTTGACAG AGTACTTCTT
    GGGCTTCTTA GTCTTGGGTT TGAAAAGTTT GTCAGAGTTG GGAGTGTCAG GAAGATTGCC
    AAGCCAATTT TACCTTACAG TTTGCATGCT GGCTCAGAAA ATGAAAATGA ACAATTAAAA
    GAACTGCATA CACTCATGAA GGAAGACCTG ACTCCTGTGG AAAGAGTCTA TGTGAGAAAA
    AGTATTGAGC AACATAAACT GGGGACCAAT AAAACCCTGC TGAAGCAGGT TCGAGTAGTT
    GGAGTTACCT GTGCAGCCTG CCCATTTCCA TGCATGAATG ACCTTAAATT TCCTGTGGTT
    GTACTGGATG AGTGTAGCCA AATAACCGAA CCAGCTTCTC TCCTTCCCAT TGCAAGGTTT
    GAGTGTGAAA AGCTTATTCT TGTTGGAGAT CCCAAACAGC TTCCTCCTAC TATTCAGGGT
    TCTGATGCAG CTCATGAAAA TGGACTGGAA CAAACTCTTT TTGATCGACT TTGCTTAATG
    GGTCACAAGC CAGTTCTTTT GCGAACCCAG TACCGTTGTC ACCCTACAAT CAGTGCTATT
    GCTAATGATC TGTTTTATGA AGGAAACCTC GTGAATGGAG TTTCAGAAAC AGAGAGGAGC
    CCTTTATTGG AATGGCTTCC AACCCTGTGT TTCTATAACA TTCAAGGGCT GGAACAGATC
    GAAAGAGATA ACAGCTTTCA TAACGTAGCA GAAGCTGCTT TTACACTCAA GCTGATTCAA
    TCACTGATTG TGAGTGGAAT AGCAGGCTCT ATGATTGGGG TGATAACATT ATACAAATCC
    CAGATGTACA AGCTTTGTCA CTTACTCACT GCACTGGACT TTGACCATCC TGGTAATGTG
    AAAGCTGTGC AGGTGTCCAC AGTAGATGCT TTTCAGGGCG CTGAAAAGGA GATCATCATT
    CTGTCCTGTG TGAGGACAAG ACAAGTAGGA TTTATTGATT CAGAACAAAG AATGAATGTT
    GCATTGACCA GAGGAAGGAG ACATTTGTTG ATTGTGGGAA ATTTGGCCTG TTTGAGGAAA
    AATCGACTAT GGGGGCGTGT GATCCAACAC TGTGAAGGCA GGGAAGATGG ATTGCAACAT
    GCAAGCCAAT ATGAACCACA GCTCAACCAT CTCCTTAAAG ATTATTTTGA AAAACAAGCA
    GAAGAAAAAC AAAAGAAAAA GAATGAAAAA GATAAATCCA AAGATAAATC TCATTTACAA
    AAAGAAACAG TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020957320.1
    CDS405..6659
    Translation

    Target ORF information:

    RefSeq Version XM_021101661.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa zinc finger GRF-type containing 1 (ZGRF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021101661.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGAAAGCC AGGAATTTAT TGTCCTATAT ACTCATCAAA AGATGAAGAA GTCAAAAGTA 
    TGGCAAGATG GAATTCTGAA GATCACTCAC GTAGGAAACA AAGCCATTTT ATATGATGAC
    AAAGGACAGT GTTTGGAGAG TTTATTTCTT AAGTGCCTTG AGGTGAAACC TGGAGATGAT
    TTAGAAAGTG ATCGATACTT AATCACAGTT GAGGAGGTTA AAGTTGCTGG AAGCATAGCT
    GTTAAACAAG ATATCATTAA AGGAGCACCT CCATTAAATT CAAAGAGCTT TATACCTTCT
    GGCCGGTCCC TTGGGTGTCA ACCTGCTGGT TTAAAAAGGA AATTTACTGG TTTTCAAGGA
    CCACGGCAAG TGCCAAAGAA AATGGCTATT ACAGAAAATA GTGAATCAGC TGCTTCACTT
    GAGGCTAAGG AAGCTGGTCC TGTTTTTCTT TCTCCAGTCT ACAGCACACC TCCTTTGTTT
    CCTACTGTTG GCAAAAAAGA TATAAATTAT ATGCCAACAG ACCCTGACAA CCAGGACAGA
    GAGAGAAATG GCATAGCTTT TTCTTCGGTT GTCTCTACTC CATCCTTCAA GATTAACCCA
    GAAATGCTAT GTGAAGAAAA TTATTTTTGC TCTACTGTCA ATTCTGTAAA TAAGCATTCA
    GGTTCTTTAC TGACCGATGA GCTTATGAAA AGAGATTGTT TGGTATCTCA CAATTCAGGA
    GTTTCACAGA ACATTAGAAG TAAAGCCCAG ATATTGGCTC TTCTGAAATC CAAATCCACT
    GGTACATCTA AAGAACCAAA TTCTGAGATT ACAGGACATT TCCCTCTGAT ACAACCACAA
    GGAAGTTTAA AAATTCCTGC TAAACTTGAG TGCTTAATTG AACAGGAAGA ACACACTCAG
    GTGAAGAGCA CAGAAAGTTT ATACTGCCAA CATCAATCAA AAAATACCAT GAGAAATAAA
    AGCCGGTGGG CCATGTATTT ATCCTCACAG AGTTCACCTA CACATTCTTC TACAGTAGAT
    GGAAATAATA CAGGAAAAAA TCCTGAGGCC GAGGGAGGTA ATATAAATTT AAATTTGAAA
    GACGCTTTGG TGCAAAAAAG CATGCGGTTT TTAGAAACAT GTACTGAAGA GGGAAAAAAG
    TATAATGAAG ACAAGCCAGT AGTTGATAAT GATCAATCCT GGAATCAAAA AGTAAAATTA
    GAATTTCCTT CATTCTGTGA AAGCAGTGCC TTACCAGTTA CTTGTGACAA CGTAGAAAAC
    GACAGCTTAT TATCAGAAAA TGACAGCTTA TTATCAGAAA ATGACATTCA AGAAAATAAT
    AAAAGACTTT TTAATCAAAA TGACCAGATG GGCATAAAAG GATCAATTCT CATTACAGAA
    AAAGCTCAGG AGATAACCAT GTATGGAACC CCAGCTAAGG AGTATAAACA TCTGCAGATT
    GAATCTTCAC TAAGTAACGA TTTTAGGGTC TCTAATGGCA TTGCTGACAT GTTTTCTAAA
    AGTAATGCTG ATAATGAAAA TCTGAACAGT ATTCATGAAC CACTTGTGGA GATTACTTTT
    AATCTGAACA ATTTTGAAAC CAGTGACTCT GAGGAGGAAT CACAGGAAAG CAGCAGAATT
    TCCCACGATT CAGAGGGTTG GGTGAAGGAA ACTTTAGTTA AAGACAATTC AAGCTGTGAG
    AATATAAACT GTGCAGAAGT TGGCAGTGAC CATTTGCCTC TCTTAACGTC TAGTGGGGAC
    AGACTTGCAG AGACATTTTC CATGAAAGAG ACTCTGCTAT CACAATTTTG TGATAAAACT
    TCTGTAGGTT TTGACACAGG ACCTTGGAAA GCTGGAAACA CTAGAAAAGA AATAGAGGAG
    GAGTACGGTG ACACCTCAAA CAATCTTGAC TCATCTTTAG AGTGGACTGA AGATGCAAAT
    GTAGGTAGTA ATGAAGGCAC TAATAAATCT ACTCAGGAAG TCACAGTTAA CTATGATTTT
    GCTTCTCTCC CAAATGAAAG TATAAACACA AATTTACATA TCCCTCACTC TTTAAACACA
    GCCACTAATC AGGATCCTGA AAACAATCCA TGGTCACAAC AGGCTCAGCC TCAGCCTTTT
    ATTATGGGAA CTGACTTACA CAAAAATACT GAACAGGTTT TACCATTAAC TTCTAGCAGT
    GGCAACGGCA CCCAACTATT AAATGCCAGT CAGAATCACT CTGAAGAATT TCTTGCACTT
    GATAAATCAC ATACCCAAGT TTCCAATTCT TCGTTTTACC CTCTGGGAGT AAAATATCCT
    ATATCTAAAG ACACAGAATC ACATATTCTG GAATCTGAAG ATTTGGGAAG GACTAGAAGT
    TTACCTCATG ACAATACTGA CATAGAAACT GCCAGAGAGA GCAAACAGTA CTGGAATACT
    CCTAAAAATT CTTCAGAACT TTCTGGATTA GAAAATAGCA TTTCCCTATT AAAATCATTG
    TCTGAACACA GTACTGCTTT AGAAGGCTTG GAAATATTGA AAAAGAAACA TACCTCCTTT
    AAGCAACAAG GAACTCTGCA GACGTATGAT CCAGAAAGCA GTCCTGAAGC TAGGAAACCA
    TTGACTACAA TAGTTTCACA AGCTATACCA AAGTCTCATC CGAACCAGGA TCCTCAACAG
    ATGATAAAAG AAAATGAAGT TAAACCAAGT GAATCCCTCA CCAAGTTTTT TCCCTTGGGC
    AGTGAAGAAG AGACTGACTC CCGAGCTCTC ATTCCTAAAC AAATGGAGAG AAAAACCTGT
    GACCCTAAGC CTGTTGAGTT TCAAGGGCAC CAAGTGAAAG GATCAGCTGC TAGTGCTGTG
    ATGGTCAGAG GACACAGCTC CCAGTTTGAA TGCAGTCAAT TTCCAGATAG CGTGTATGAA
    AACTGCATGA CAAATACTTG TTTTTTGACA CCTAAGTTGC CTAGCACTTG TATGCAGATT
    GACTTCTTGC AGTTGCCAGA TGAAGAAAAC TTGATAAAAG GAGATTCCAG CTTTAACACT
    GAAGACAATT TTCAAGTGAT AGCTGAGCCT AATAAGAATT CAGTTAAAAA TGATCTAATG
    AAAAAGTCTC TGGAGAATAA GAACCTTCAA AGGCTTTCAT TAGTAAGTAG AATCCGAGTT
    CCACTTATTA CTTTGCCAGC TACCAGTGGG TCACCTGACC TAGATTCTTG TTCGTGTGTG
    ATCGACTGTG ACAGGCAAGA GTCTTCTGAT TCTCCCATAG TCAACTTGTA TGAAGAGTCA
    GAGCTTCCTT CTTTCAGCTT TGGATCTGAG GACCAAAGTG AAACTTCTTT CTCTAAAGAA
    TCTACTGAAA TGACTCCAAG GGATATTTCA CTTCTCGATA ATACATCTAC TCAAAGCAAG
    TGGCTGAAAT ATCAAAACAT AATCCAATGC AAGTTGACTA CACCAAACAG ACTTGATCAG
    AAAGTAACAG ATGGCTTCTT TGCTGAGGCT GTTTCCAGGA TGCATTTTAG TGACACAGGT
    GAAAGACAGA GTGATGCTGT GAATGAAAGC TTAGACTCTG AGCATTTGCA AATGATAAAA
    TGCATGCTTC AACAACAGCG GCAGGATTTT AGCAGTCAAG ATTTGGTTTC CAGACAGAAA
    GCACTCTCTT TGAATTTAAA GCACACTTCT AAGACTGAGG AAATTAAAAA TGTGTTAGGA
    GAGTCTGCCT GCTACACCTG CAGTGTAAGA GGAGATTTAC AGGAGATAAG TGACTGTGAG
    CTGTGCTTCC CAAGTGGGGA GAAAGTAAAA TCTGCATATC TTCCCCAAAG GCAAATTCAC
    ATACCAGCTG TTTTTCAGTC TCCTGCTCAT TATAAACAGA TTTTTACATC TTGTCTCATA
    GAACATCTAA ATATATTGCT GTTTGGGTTG GCACAAAGGC TGCACAAAGC TCTTTCAAAA
    GTTGACATAT CATTTTACTC ATCAAACGGA GACAAACGGA AAAATGTAGA AAATAATGTG
    CCATCATGCC ATCATCATCA CCCTGCAAAA CTTGTCATGG TTAAAAAGGA AGGTCCAAAT
    AAGGGTCGTC TCTTTTATGC TTGTGATGGA CCCAAAGCTG ACCAATGTAA ATTTTTCAAG
    TGGCTTGATG AAGTGACCCC AGGATATTTA ACACAGGAAG AATCACTACC TAGCATGGTT
    TTAAGTGATA TAAAAAGTAT TGGCTTGTAC TTAAGAAGTC AAAAGATACC CCTCTATGAG
    GAATGCCAAC TTTTGGTGAG AAAAGGATTT GATTTTCAAA GAAAACGGTA TGGTAAACTA
    AAGAAGTTTA CAACTGTAGA TCCTGAATTT TATAACGAAC CTAAGAGCAG ACTTTATCTT
    AAGCTGAGTC GTAAGGAAAG TTCATCTGCT TATAGTAAAG ATGATCTTTG GGTGGTTTCA
    AAAACCCTAG ACTTTGAATT GGATACTTTT ATTGCATGTA GTACATTCTT TGGACCGTCA
    TCTGTCAATG AGGTAGAGCT ACTACCTTTG AAAGGTTATT TCCCTTCCAA CTGGCCCACT
    AACACATTGG TCCATGCATT ATTGGTTTGT AATGCTAGCA CGGAGCTGAC CACTTTGAAA
    AATATTCAGG ACTACTTTAA TCCAGCTACT CTGCCTCTAA CACAGAACCT GTTAACAATG
    TCTTCATCAA CTATAGTGAG CAACAAGAGA GTCAATAAGA GAAAATTTAT CCCACCAGCC
    TTCTCAAATA TCAATACAAA ATTTGAACTG CTCAGTATAG GAGCAACGCT GCAGTTAGCT
    GGTGAGTTAA TTCACACACA CAAGTTAAAC AAGGATCAAG CTACAGCCCT AATTCAGATA
    GCGCAAATGA TGGCATCACA AGGAAGTGCT GAAGAAGTGA AGGAACTGGG AACTCACACC
    CTCCCAATCA CGATCATACA TGGTGTTTTT GGAGCAGGAA AAAGTTATTT GCTGGCAGTG
    GTGATTTTGT TTTTTGTTCA GCTATTTGAA AAGAGTGAAG CTCTTGCAGT TGGAAATGGA
    AGACCTTGGA AACTTCTGAT TTCTTCTTCT ACTAATGTAG CTGTTGACAG AGTACTTCTT
    GGGCTTCTTA GTCTTGGGTT TGAAAAGTTT GTCAGAGTTG GGAGTGTCAG GAAGATTGCC
    AAGCCAATTT TACCTTACAG TTTGCATGCT GGCTCAGAAA ATGAAAATGA ACAATTAAAA
    GAACTGCATA CACTCATGAA GGAAGACCTG ACTCCTGTGG AAAGAGTCTA TGTGAGAAAA
    AGTATTGAGC AACATAAACT GGGGACCAAT AAAACCCTGC TGAAGCAGGT TCGAGTAGTT
    GGAGTTACCT GTGCAGCCTG CCCATTTCCA TGCATGAATG ACCTTAAATT TCCTGTGGTT
    GTACTGGATG AGTGTAGCCA AATAACCGAA CCAGCTTCTC TCCTTCCCAT TGCAAGGTTT
    GAGTGTGAAA AGCTTATTCT TGTTGGAGAT CCCAAACAGC TTCCTCCTAC TATTCAGGGT
    TCTGATGCAG CTCATGAAAA TGGACTGGAA CAAACTCTTT TTGATCGACT TTGCTTAATG
    GGTCACAAGC CAGTTCTTTT GCGAACCCAG TACCGTTGTC ACCCTACAAT CAGTGCTATT
    GCTAATGATC TGTTTTATGA AGGAAACCTC GTGAATGGAG TTTCAGAAAC AGAGAGGAGC
    CCTTTATTGG AATGGCTTCC AACCCTGTGT TTCTATAACA TTCAAGGGCT GGAACAGATC
    GAAAGAGATA ACAGCTTTCA TAACGTAGCA GAAGCTGCTT TTACACTCAA GCTGATTCAA
    TCACTGATTG TGAGTGGAAT AGCAGGCTCT ATGATTGGGG TGATAACATT ATACAAATCC
    CAGATGTACA AGCTTTGTCA CTTACTCACT GCACTGGACT TTGACCATCC TGGTAATGTG
    AAAGCTGTGC AGGTGTCCAC AGTAGATGCT TTTCAGGGCG CTGAAAAGGA GATCATCATT
    CTGTCCTGTG TGAGGACAAG ACAAGTAGGA TTTATTGATT CAGAACAAAG AATGAATGTT
    GCATTGACCA GAGGAAGGAG ACATTTGTTG ATTGTGGGAA ATTTGGCCTG TTTGAGGAAA
    AATCGACTAT GGGGGCGTGT GATCCAACAC TGTGAAGGCA GGGAAGATGG ATTGCAACAT
    GCAAGCCAAT ATGAACCACA GCTCAACCAT CTCCTTAAAG ATTATTTTGA AAAACAAGCA
    GAAGAAAAAC AAAAGAAAAA GAATGAAAAA GATAAATCCA AAGATAAATC TCATTTACAA
    AAAGAAACAG TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe224826
    Clone ID Related Accession (Same CDS sequence) XM_021101661.1 , XM_021101663.1 , XM_021101662.1
    Accession Version XM_021101663.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6255bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product protein ZGRF1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010450.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGAAAGCC AGGAATTTAT TGTCCTATAT ACTCATCAAA AGATGAAGAA GTCAAAAGTA 
    TGGCAAGATG GAATTCTGAA GATCACTCAC GTAGGAAACA AAGCCATTTT ATATGATGAC
    AAAGGACAGT GTTTGGAGAG TTTATTTCTT AAGTGCCTTG AGGTGAAACC TGGAGATGAT
    TTAGAAAGTG ATCGATACTT AATCACAGTT GAGGAGGTTA AAGTTGCTGG AAGCATAGCT
    GTTAAACAAG ATATCATTAA AGGAGCACCT CCATTAAATT CAAAGAGCTT TATACCTTCT
    GGCCGGTCCC TTGGGTGTCA ACCTGCTGGT TTAAAAAGGA AATTTACTGG TTTTCAAGGA
    CCACGGCAAG TGCCAAAGAA AATGGCTATT ACAGAAAATA GTGAATCAGC TGCTTCACTT
    GAGGCTAAGG AAGCTGGTCC TGTTTTTCTT TCTCCAGTCT ACAGCACACC TCCTTTGTTT
    CCTACTGTTG GCAAAAAAGA TATAAATTAT ATGCCAACAG ACCCTGACAA CCAGGACAGA
    GAGAGAAATG GCATAGCTTT TTCTTCGGTT GTCTCTACTC CATCCTTCAA GATTAACCCA
    GAAATGCTAT GTGAAGAAAA TTATTTTTGC TCTACTGTCA ATTCTGTAAA TAAGCATTCA
    GGTTCTTTAC TGACCGATGA GCTTATGAAA AGAGATTGTT TGGTATCTCA CAATTCAGGA
    GTTTCACAGA ACATTAGAAG TAAAGCCCAG ATATTGGCTC TTCTGAAATC CAAATCCACT
    GGTACATCTA AAGAACCAAA TTCTGAGATT ACAGGACATT TCCCTCTGAT ACAACCACAA
    GGAAGTTTAA AAATTCCTGC TAAACTTGAG TGCTTAATTG AACAGGAAGA ACACACTCAG
    GTGAAGAGCA CAGAAAGTTT ATACTGCCAA CATCAATCAA AAAATACCAT GAGAAATAAA
    AGCCGGTGGG CCATGTATTT ATCCTCACAG AGTTCACCTA CACATTCTTC TACAGTAGAT
    GGAAATAATA CAGGAAAAAA TCCTGAGGCC GAGGGAGGTA ATATAAATTT AAATTTGAAA
    GACGCTTTGG TGCAAAAAAG CATGCGGTTT TTAGAAACAT GTACTGAAGA GGGAAAAAAG
    TATAATGAAG ACAAGCCAGT AGTTGATAAT GATCAATCCT GGAATCAAAA AGTAAAATTA
    GAATTTCCTT CATTCTGTGA AAGCAGTGCC TTACCAGTTA CTTGTGACAA CGTAGAAAAC
    GACAGCTTAT TATCAGAAAA TGACAGCTTA TTATCAGAAA ATGACATTCA AGAAAATAAT
    AAAAGACTTT TTAATCAAAA TGACCAGATG GGCATAAAAG GATCAATTCT CATTACAGAA
    AAAGCTCAGG AGATAACCAT GTATGGAACC CCAGCTAAGG AGTATAAACA TCTGCAGATT
    GAATCTTCAC TAAGTAACGA TTTTAGGGTC TCTAATGGCA TTGCTGACAT GTTTTCTAAA
    AGTAATGCTG ATAATGAAAA TCTGAACAGT ATTCATGAAC CACTTGTGGA GATTACTTTT
    AATCTGAACA ATTTTGAAAC CAGTGACTCT GAGGAGGAAT CACAGGAAAG CAGCAGAATT
    TCCCACGATT CAGAGGGTTG GGTGAAGGAA ACTTTAGTTA AAGACAATTC AAGCTGTGAG
    AATATAAACT GTGCAGAAGT TGGCAGTGAC CATTTGCCTC TCTTAACGTC TAGTGGGGAC
    AGACTTGCAG AGACATTTTC CATGAAAGAG ACTCTGCTAT CACAATTTTG TGATAAAACT
    TCTGTAGGTT TTGACACAGG ACCTTGGAAA GCTGGAAACA CTAGAAAAGA AATAGAGGAG
    GAGTACGGTG ACACCTCAAA CAATCTTGAC TCATCTTTAG AGTGGACTGA AGATGCAAAT
    GTAGGTAGTA ATGAAGGCAC TAATAAATCT ACTCAGGAAG TCACAGTTAA CTATGATTTT
    GCTTCTCTCC CAAATGAAAG TATAAACACA AATTTACATA TCCCTCACTC TTTAAACACA
    GCCACTAATC AGGATCCTGA AAACAATCCA TGGTCACAAC AGGCTCAGCC TCAGCCTTTT
    ATTATGGGAA CTGACTTACA CAAAAATACT GAACAGGTTT TACCATTAAC TTCTAGCAGT
    GGCAACGGCA CCCAACTATT AAATGCCAGT CAGAATCACT CTGAAGAATT TCTTGCACTT
    GATAAATCAC ATACCCAAGT TTCCAATTCT TCGTTTTACC CTCTGGGAGT AAAATATCCT
    ATATCTAAAG ACACAGAATC ACATATTCTG GAATCTGAAG ATTTGGGAAG GACTAGAAGT
    TTACCTCATG ACAATACTGA CATAGAAACT GCCAGAGAGA GCAAACAGTA CTGGAATACT
    CCTAAAAATT CTTCAGAACT TTCTGGATTA GAAAATAGCA TTTCCCTATT AAAATCATTG
    TCTGAACACA GTACTGCTTT AGAAGGCTTG GAAATATTGA AAAAGAAACA TACCTCCTTT
    AAGCAACAAG GAACTCTGCA GACGTATGAT CCAGAAAGCA GTCCTGAAGC TAGGAAACCA
    TTGACTACAA TAGTTTCACA AGCTATACCA AAGTCTCATC CGAACCAGGA TCCTCAACAG
    ATGATAAAAG AAAATGAAGT TAAACCAAGT GAATCCCTCA CCAAGTTTTT TCCCTTGGGC
    AGTGAAGAAG AGACTGACTC CCGAGCTCTC ATTCCTAAAC AAATGGAGAG AAAAACCTGT
    GACCCTAAGC CTGTTGAGTT TCAAGGGCAC CAAGTGAAAG GATCAGCTGC TAGTGCTGTG
    ATGGTCAGAG GACACAGCTC CCAGTTTGAA TGCAGTCAAT TTCCAGATAG CGTGTATGAA
    AACTGCATGA CAAATACTTG TTTTTTGACA CCTAAGTTGC CTAGCACTTG TATGCAGATT
    GACTTCTTGC AGTTGCCAGA TGAAGAAAAC TTGATAAAAG GAGATTCCAG CTTTAACACT
    GAAGACAATT TTCAAGTGAT AGCTGAGCCT AATAAGAATT CAGTTAAAAA TGATCTAATG
    AAAAAGTCTC TGGAGAATAA GAACCTTCAA AGGCTTTCAT TAGTAAGTAG AATCCGAGTT
    CCACTTATTA CTTTGCCAGC TACCAGTGGG TCACCTGACC TAGATTCTTG TTCGTGTGTG
    ATCGACTGTG ACAGGCAAGA GTCTTCTGAT TCTCCCATAG TCAACTTGTA TGAAGAGTCA
    GAGCTTCCTT CTTTCAGCTT TGGATCTGAG GACCAAAGTG AAACTTCTTT CTCTAAAGAA
    TCTACTGAAA TGACTCCAAG GGATATTTCA CTTCTCGATA ATACATCTAC TCAAAGCAAG
    TGGCTGAAAT ATCAAAACAT AATCCAATGC AAGTTGACTA CACCAAACAG ACTTGATCAG
    AAAGTAACAG ATGGCTTCTT TGCTGAGGCT GTTTCCAGGA TGCATTTTAG TGACACAGGT
    GAAAGACAGA GTGATGCTGT GAATGAAAGC TTAGACTCTG AGCATTTGCA AATGATAAAA
    TGCATGCTTC AACAACAGCG GCAGGATTTT AGCAGTCAAG ATTTGGTTTC CAGACAGAAA
    GCACTCTCTT TGAATTTAAA GCACACTTCT AAGACTGAGG AAATTAAAAA TGTGTTAGGA
    GAGTCTGCCT GCTACACCTG CAGTGTAAGA GGAGATTTAC AGGAGATAAG TGACTGTGAG
    CTGTGCTTCC CAAGTGGGGA GAAAGTAAAA TCTGCATATC TTCCCCAAAG GCAAATTCAC
    ATACCAGCTG TTTTTCAGTC TCCTGCTCAT TATAAACAGA TTTTTACATC TTGTCTCATA
    GAACATCTAA ATATATTGCT GTTTGGGTTG GCACAAAGGC TGCACAAAGC TCTTTCAAAA
    GTTGACATAT CATTTTACTC ATCAAACGGA GACAAACGGA AAAATGTAGA AAATAATGTG
    CCATCATGCC ATCATCATCA CCCTGCAAAA CTTGTCATGG TTAAAAAGGA AGGTCCAAAT
    AAGGGTCGTC TCTTTTATGC TTGTGATGGA CCCAAAGCTG ACCAATGTAA ATTTTTCAAG
    TGGCTTGATG AAGTGACCCC AGGATATTTA ACACAGGAAG AATCACTACC TAGCATGGTT
    TTAAGTGATA TAAAAAGTAT TGGCTTGTAC TTAAGAAGTC AAAAGATACC CCTCTATGAG
    GAATGCCAAC TTTTGGTGAG AAAAGGATTT GATTTTCAAA GAAAACGGTA TGGTAAACTA
    AAGAAGTTTA CAACTGTAGA TCCTGAATTT TATAACGAAC CTAAGAGCAG ACTTTATCTT
    AAGCTGAGTC GTAAGGAAAG TTCATCTGCT TATAGTAAAG ATGATCTTTG GGTGGTTTCA
    AAAACCCTAG ACTTTGAATT GGATACTTTT ATTGCATGTA GTACATTCTT TGGACCGTCA
    TCTGTCAATG AGGTAGAGCT ACTACCTTTG AAAGGTTATT TCCCTTCCAA CTGGCCCACT
    AACACATTGG TCCATGCATT ATTGGTTTGT AATGCTAGCA CGGAGCTGAC CACTTTGAAA
    AATATTCAGG ACTACTTTAA TCCAGCTACT CTGCCTCTAA CACAGAACCT GTTAACAATG
    TCTTCATCAA CTATAGTGAG CAACAAGAGA GTCAATAAGA GAAAATTTAT CCCACCAGCC
    TTCTCAAATA TCAATACAAA ATTTGAACTG CTCAGTATAG GAGCAACGCT GCAGTTAGCT
    GGTGAGTTAA TTCACACACA CAAGTTAAAC AAGGATCAAG CTACAGCCCT AATTCAGATA
    GCGCAAATGA TGGCATCACA AGGAAGTGCT GAAGAAGTGA AGGAACTGGG AACTCACACC
    CTCCCAATCA CGATCATACA TGGTGTTTTT GGAGCAGGAA AAAGTTATTT GCTGGCAGTG
    GTGATTTTGT TTTTTGTTCA GCTATTTGAA AAGAGTGAAG CTCTTGCAGT TGGAAATGGA
    AGACCTTGGA AACTTCTGAT TTCTTCTTCT ACTAATGTAG CTGTTGACAG AGTACTTCTT
    GGGCTTCTTA GTCTTGGGTT TGAAAAGTTT GTCAGAGTTG GGAGTGTCAG GAAGATTGCC
    AAGCCAATTT TACCTTACAG TTTGCATGCT GGCTCAGAAA ATGAAAATGA ACAATTAAAA
    GAACTGCATA CACTCATGAA GGAAGACCTG ACTCCTGTGG AAAGAGTCTA TGTGAGAAAA
    AGTATTGAGC AACATAAACT GGGGACCAAT AAAACCCTGC TGAAGCAGGT TCGAGTAGTT
    GGAGTTACCT GTGCAGCCTG CCCATTTCCA TGCATGAATG ACCTTAAATT TCCTGTGGTT
    GTACTGGATG AGTGTAGCCA AATAACCGAA CCAGCTTCTC TCCTTCCCAT TGCAAGGTTT
    GAGTGTGAAA AGCTTATTCT TGTTGGAGAT CCCAAACAGC TTCCTCCTAC TATTCAGGGT
    TCTGATGCAG CTCATGAAAA TGGACTGGAA CAAACTCTTT TTGATCGACT TTGCTTAATG
    GGTCACAAGC CAGTTCTTTT GCGAACCCAG TACCGTTGTC ACCCTACAAT CAGTGCTATT
    GCTAATGATC TGTTTTATGA AGGAAACCTC GTGAATGGAG TTTCAGAAAC AGAGAGGAGC
    CCTTTATTGG AATGGCTTCC AACCCTGTGT TTCTATAACA TTCAAGGGCT GGAACAGATC
    GAAAGAGATA ACAGCTTTCA TAACGTAGCA GAAGCTGCTT TTACACTCAA GCTGATTCAA
    TCACTGATTG TGAGTGGAAT AGCAGGCTCT ATGATTGGGG TGATAACATT ATACAAATCC
    CAGATGTACA AGCTTTGTCA CTTACTCACT GCACTGGACT TTGACCATCC TGGTAATGTG
    AAAGCTGTGC AGGTGTCCAC AGTAGATGCT TTTCAGGGCG CTGAAAAGGA GATCATCATT
    CTGTCCTGTG TGAGGACAAG ACAAGTAGGA TTTATTGATT CAGAACAAAG AATGAATGTT
    GCATTGACCA GAGGAAGGAG ACATTTGTTG ATTGTGGGAA ATTTGGCCTG TTTGAGGAAA
    AATCGACTAT GGGGGCGTGT GATCCAACAC TGTGAAGGCA GGGAAGATGG ATTGCAACAT
    GCAAGCCAAT ATGAACCACA GCTCAACCAT CTCCTTAAAG ATTATTTTGA AAAACAAGCA
    GAAGAAAAAC AAAAGAAAAA GAATGAAAAA GATAAATCCA AAGATAAATC TCATTTACAA
    AAAGAAACAG TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020957322.1
    CDS134..6388
    Translation

    Target ORF information:

    RefSeq Version XM_021101663.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa zinc finger GRF-type containing 1 (ZGRF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021101663.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGAAAGCC AGGAATTTAT TGTCCTATAT ACTCATCAAA AGATGAAGAA GTCAAAAGTA 
    TGGCAAGATG GAATTCTGAA GATCACTCAC GTAGGAAACA AAGCCATTTT ATATGATGAC
    AAAGGACAGT GTTTGGAGAG TTTATTTCTT AAGTGCCTTG AGGTGAAACC TGGAGATGAT
    TTAGAAAGTG ATCGATACTT AATCACAGTT GAGGAGGTTA AAGTTGCTGG AAGCATAGCT
    GTTAAACAAG ATATCATTAA AGGAGCACCT CCATTAAATT CAAAGAGCTT TATACCTTCT
    GGCCGGTCCC TTGGGTGTCA ACCTGCTGGT TTAAAAAGGA AATTTACTGG TTTTCAAGGA
    CCACGGCAAG TGCCAAAGAA AATGGCTATT ACAGAAAATA GTGAATCAGC TGCTTCACTT
    GAGGCTAAGG AAGCTGGTCC TGTTTTTCTT TCTCCAGTCT ACAGCACACC TCCTTTGTTT
    CCTACTGTTG GCAAAAAAGA TATAAATTAT ATGCCAACAG ACCCTGACAA CCAGGACAGA
    GAGAGAAATG GCATAGCTTT TTCTTCGGTT GTCTCTACTC CATCCTTCAA GATTAACCCA
    GAAATGCTAT GTGAAGAAAA TTATTTTTGC TCTACTGTCA ATTCTGTAAA TAAGCATTCA
    GGTTCTTTAC TGACCGATGA GCTTATGAAA AGAGATTGTT TGGTATCTCA CAATTCAGGA
    GTTTCACAGA ACATTAGAAG TAAAGCCCAG ATATTGGCTC TTCTGAAATC CAAATCCACT
    GGTACATCTA AAGAACCAAA TTCTGAGATT ACAGGACATT TCCCTCTGAT ACAACCACAA
    GGAAGTTTAA AAATTCCTGC TAAACTTGAG TGCTTAATTG AACAGGAAGA ACACACTCAG
    GTGAAGAGCA CAGAAAGTTT ATACTGCCAA CATCAATCAA AAAATACCAT GAGAAATAAA
    AGCCGGTGGG CCATGTATTT ATCCTCACAG AGTTCACCTA CACATTCTTC TACAGTAGAT
    GGAAATAATA CAGGAAAAAA TCCTGAGGCC GAGGGAGGTA ATATAAATTT AAATTTGAAA
    GACGCTTTGG TGCAAAAAAG CATGCGGTTT TTAGAAACAT GTACTGAAGA GGGAAAAAAG
    TATAATGAAG ACAAGCCAGT AGTTGATAAT GATCAATCCT GGAATCAAAA AGTAAAATTA
    GAATTTCCTT CATTCTGTGA AAGCAGTGCC TTACCAGTTA CTTGTGACAA CGTAGAAAAC
    GACAGCTTAT TATCAGAAAA TGACAGCTTA TTATCAGAAA ATGACATTCA AGAAAATAAT
    AAAAGACTTT TTAATCAAAA TGACCAGATG GGCATAAAAG GATCAATTCT CATTACAGAA
    AAAGCTCAGG AGATAACCAT GTATGGAACC CCAGCTAAGG AGTATAAACA TCTGCAGATT
    GAATCTTCAC TAAGTAACGA TTTTAGGGTC TCTAATGGCA TTGCTGACAT GTTTTCTAAA
    AGTAATGCTG ATAATGAAAA TCTGAACAGT ATTCATGAAC CACTTGTGGA GATTACTTTT
    AATCTGAACA ATTTTGAAAC CAGTGACTCT GAGGAGGAAT CACAGGAAAG CAGCAGAATT
    TCCCACGATT CAGAGGGTTG GGTGAAGGAA ACTTTAGTTA AAGACAATTC AAGCTGTGAG
    AATATAAACT GTGCAGAAGT TGGCAGTGAC CATTTGCCTC TCTTAACGTC TAGTGGGGAC
    AGACTTGCAG AGACATTTTC CATGAAAGAG ACTCTGCTAT CACAATTTTG TGATAAAACT
    TCTGTAGGTT TTGACACAGG ACCTTGGAAA GCTGGAAACA CTAGAAAAGA AATAGAGGAG
    GAGTACGGTG ACACCTCAAA CAATCTTGAC TCATCTTTAG AGTGGACTGA AGATGCAAAT
    GTAGGTAGTA ATGAAGGCAC TAATAAATCT ACTCAGGAAG TCACAGTTAA CTATGATTTT
    GCTTCTCTCC CAAATGAAAG TATAAACACA AATTTACATA TCCCTCACTC TTTAAACACA
    GCCACTAATC AGGATCCTGA AAACAATCCA TGGTCACAAC AGGCTCAGCC TCAGCCTTTT
    ATTATGGGAA CTGACTTACA CAAAAATACT GAACAGGTTT TACCATTAAC TTCTAGCAGT
    GGCAACGGCA CCCAACTATT AAATGCCAGT CAGAATCACT CTGAAGAATT TCTTGCACTT
    GATAAATCAC ATACCCAAGT TTCCAATTCT TCGTTTTACC CTCTGGGAGT AAAATATCCT
    ATATCTAAAG ACACAGAATC ACATATTCTG GAATCTGAAG ATTTGGGAAG GACTAGAAGT
    TTACCTCATG ACAATACTGA CATAGAAACT GCCAGAGAGA GCAAACAGTA CTGGAATACT
    CCTAAAAATT CTTCAGAACT TTCTGGATTA GAAAATAGCA TTTCCCTATT AAAATCATTG
    TCTGAACACA GTACTGCTTT AGAAGGCTTG GAAATATTGA AAAAGAAACA TACCTCCTTT
    AAGCAACAAG GAACTCTGCA GACGTATGAT CCAGAAAGCA GTCCTGAAGC TAGGAAACCA
    TTGACTACAA TAGTTTCACA AGCTATACCA AAGTCTCATC CGAACCAGGA TCCTCAACAG
    ATGATAAAAG AAAATGAAGT TAAACCAAGT GAATCCCTCA CCAAGTTTTT TCCCTTGGGC
    AGTGAAGAAG AGACTGACTC CCGAGCTCTC ATTCCTAAAC AAATGGAGAG AAAAACCTGT
    GACCCTAAGC CTGTTGAGTT TCAAGGGCAC CAAGTGAAAG GATCAGCTGC TAGTGCTGTG
    ATGGTCAGAG GACACAGCTC CCAGTTTGAA TGCAGTCAAT TTCCAGATAG CGTGTATGAA
    AACTGCATGA CAAATACTTG TTTTTTGACA CCTAAGTTGC CTAGCACTTG TATGCAGATT
    GACTTCTTGC AGTTGCCAGA TGAAGAAAAC TTGATAAAAG GAGATTCCAG CTTTAACACT
    GAAGACAATT TTCAAGTGAT AGCTGAGCCT AATAAGAATT CAGTTAAAAA TGATCTAATG
    AAAAAGTCTC TGGAGAATAA GAACCTTCAA AGGCTTTCAT TAGTAAGTAG AATCCGAGTT
    CCACTTATTA CTTTGCCAGC TACCAGTGGG TCACCTGACC TAGATTCTTG TTCGTGTGTG
    ATCGACTGTG ACAGGCAAGA GTCTTCTGAT TCTCCCATAG TCAACTTGTA TGAAGAGTCA
    GAGCTTCCTT CTTTCAGCTT TGGATCTGAG GACCAAAGTG AAACTTCTTT CTCTAAAGAA
    TCTACTGAAA TGACTCCAAG GGATATTTCA CTTCTCGATA ATACATCTAC TCAAAGCAAG
    TGGCTGAAAT ATCAAAACAT AATCCAATGC AAGTTGACTA CACCAAACAG ACTTGATCAG
    AAAGTAACAG ATGGCTTCTT TGCTGAGGCT GTTTCCAGGA TGCATTTTAG TGACACAGGT
    GAAAGACAGA GTGATGCTGT GAATGAAAGC TTAGACTCTG AGCATTTGCA AATGATAAAA
    TGCATGCTTC AACAACAGCG GCAGGATTTT AGCAGTCAAG ATTTGGTTTC CAGACAGAAA
    GCACTCTCTT TGAATTTAAA GCACACTTCT AAGACTGAGG AAATTAAAAA TGTGTTAGGA
    GAGTCTGCCT GCTACACCTG CAGTGTAAGA GGAGATTTAC AGGAGATAAG TGACTGTGAG
    CTGTGCTTCC CAAGTGGGGA GAAAGTAAAA TCTGCATATC TTCCCCAAAG GCAAATTCAC
    ATACCAGCTG TTTTTCAGTC TCCTGCTCAT TATAAACAGA TTTTTACATC TTGTCTCATA
    GAACATCTAA ATATATTGCT GTTTGGGTTG GCACAAAGGC TGCACAAAGC TCTTTCAAAA
    GTTGACATAT CATTTTACTC ATCAAACGGA GACAAACGGA AAAATGTAGA AAATAATGTG
    CCATCATGCC ATCATCATCA CCCTGCAAAA CTTGTCATGG TTAAAAAGGA AGGTCCAAAT
    AAGGGTCGTC TCTTTTATGC TTGTGATGGA CCCAAAGCTG ACCAATGTAA ATTTTTCAAG
    TGGCTTGATG AAGTGACCCC AGGATATTTA ACACAGGAAG AATCACTACC TAGCATGGTT
    TTAAGTGATA TAAAAAGTAT TGGCTTGTAC TTAAGAAGTC AAAAGATACC CCTCTATGAG
    GAATGCCAAC TTTTGGTGAG AAAAGGATTT GATTTTCAAA GAAAACGGTA TGGTAAACTA
    AAGAAGTTTA CAACTGTAGA TCCTGAATTT TATAACGAAC CTAAGAGCAG ACTTTATCTT
    AAGCTGAGTC GTAAGGAAAG TTCATCTGCT TATAGTAAAG ATGATCTTTG GGTGGTTTCA
    AAAACCCTAG ACTTTGAATT GGATACTTTT ATTGCATGTA GTACATTCTT TGGACCGTCA
    TCTGTCAATG AGGTAGAGCT ACTACCTTTG AAAGGTTATT TCCCTTCCAA CTGGCCCACT
    AACACATTGG TCCATGCATT ATTGGTTTGT AATGCTAGCA CGGAGCTGAC CACTTTGAAA
    AATATTCAGG ACTACTTTAA TCCAGCTACT CTGCCTCTAA CACAGAACCT GTTAACAATG
    TCTTCATCAA CTATAGTGAG CAACAAGAGA GTCAATAAGA GAAAATTTAT CCCACCAGCC
    TTCTCAAATA TCAATACAAA ATTTGAACTG CTCAGTATAG GAGCAACGCT GCAGTTAGCT
    GGTGAGTTAA TTCACACACA CAAGTTAAAC AAGGATCAAG CTACAGCCCT AATTCAGATA
    GCGCAAATGA TGGCATCACA AGGAAGTGCT GAAGAAGTGA AGGAACTGGG AACTCACACC
    CTCCCAATCA CGATCATACA TGGTGTTTTT GGAGCAGGAA AAAGTTATTT GCTGGCAGTG
    GTGATTTTGT TTTTTGTTCA GCTATTTGAA AAGAGTGAAG CTCTTGCAGT TGGAAATGGA
    AGACCTTGGA AACTTCTGAT TTCTTCTTCT ACTAATGTAG CTGTTGACAG AGTACTTCTT
    GGGCTTCTTA GTCTTGGGTT TGAAAAGTTT GTCAGAGTTG GGAGTGTCAG GAAGATTGCC
    AAGCCAATTT TACCTTACAG TTTGCATGCT GGCTCAGAAA ATGAAAATGA ACAATTAAAA
    GAACTGCATA CACTCATGAA GGAAGACCTG ACTCCTGTGG AAAGAGTCTA TGTGAGAAAA
    AGTATTGAGC AACATAAACT GGGGACCAAT AAAACCCTGC TGAAGCAGGT TCGAGTAGTT
    GGAGTTACCT GTGCAGCCTG CCCATTTCCA TGCATGAATG ACCTTAAATT TCCTGTGGTT
    GTACTGGATG AGTGTAGCCA AATAACCGAA CCAGCTTCTC TCCTTCCCAT TGCAAGGTTT
    GAGTGTGAAA AGCTTATTCT TGTTGGAGAT CCCAAACAGC TTCCTCCTAC TATTCAGGGT
    TCTGATGCAG CTCATGAAAA TGGACTGGAA CAAACTCTTT TTGATCGACT TTGCTTAATG
    GGTCACAAGC CAGTTCTTTT GCGAACCCAG TACCGTTGTC ACCCTACAAT CAGTGCTATT
    GCTAATGATC TGTTTTATGA AGGAAACCTC GTGAATGGAG TTTCAGAAAC AGAGAGGAGC
    CCTTTATTGG AATGGCTTCC AACCCTGTGT TTCTATAACA TTCAAGGGCT GGAACAGATC
    GAAAGAGATA ACAGCTTTCA TAACGTAGCA GAAGCTGCTT TTACACTCAA GCTGATTCAA
    TCACTGATTG TGAGTGGAAT AGCAGGCTCT ATGATTGGGG TGATAACATT ATACAAATCC
    CAGATGTACA AGCTTTGTCA CTTACTCACT GCACTGGACT TTGACCATCC TGGTAATGTG
    AAAGCTGTGC AGGTGTCCAC AGTAGATGCT TTTCAGGGCG CTGAAAAGGA GATCATCATT
    CTGTCCTGTG TGAGGACAAG ACAAGTAGGA TTTATTGATT CAGAACAAAG AATGAATGTT
    GCATTGACCA GAGGAAGGAG ACATTTGTTG ATTGTGGGAA ATTTGGCCTG TTTGAGGAAA
    AATCGACTAT GGGGGCGTGT GATCCAACAC TGTGAAGGCA GGGAAGATGG ATTGCAACAT
    GCAAGCCAAT ATGAACCACA GCTCAACCAT CTCCTTAAAG ATTATTTTGA AAAACAAGCA
    GAAGAAAAAC AAAAGAAAAA GAATGAAAAA GATAAATCCA AAGATAAATC TCATTTACAA
    AAAGAAACAG TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe224826
    Clone ID Related Accession (Same CDS sequence) XM_021101661.1 , XM_021101663.1 , XM_021101662.1
    Accession Version XM_021101662.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6255bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product protein ZGRF1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010450.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGAAAGCC AGGAATTTAT TGTCCTATAT ACTCATCAAA AGATGAAGAA GTCAAAAGTA 
    TGGCAAGATG GAATTCTGAA GATCACTCAC GTAGGAAACA AAGCCATTTT ATATGATGAC
    AAAGGACAGT GTTTGGAGAG TTTATTTCTT AAGTGCCTTG AGGTGAAACC TGGAGATGAT
    TTAGAAAGTG ATCGATACTT AATCACAGTT GAGGAGGTTA AAGTTGCTGG AAGCATAGCT
    GTTAAACAAG ATATCATTAA AGGAGCACCT CCATTAAATT CAAAGAGCTT TATACCTTCT
    GGCCGGTCCC TTGGGTGTCA ACCTGCTGGT TTAAAAAGGA AATTTACTGG TTTTCAAGGA
    CCACGGCAAG TGCCAAAGAA AATGGCTATT ACAGAAAATA GTGAATCAGC TGCTTCACTT
    GAGGCTAAGG AAGCTGGTCC TGTTTTTCTT TCTCCAGTCT ACAGCACACC TCCTTTGTTT
    CCTACTGTTG GCAAAAAAGA TATAAATTAT ATGCCAACAG ACCCTGACAA CCAGGACAGA
    GAGAGAAATG GCATAGCTTT TTCTTCGGTT GTCTCTACTC CATCCTTCAA GATTAACCCA
    GAAATGCTAT GTGAAGAAAA TTATTTTTGC TCTACTGTCA ATTCTGTAAA TAAGCATTCA
    GGTTCTTTAC TGACCGATGA GCTTATGAAA AGAGATTGTT TGGTATCTCA CAATTCAGGA
    GTTTCACAGA ACATTAGAAG TAAAGCCCAG ATATTGGCTC TTCTGAAATC CAAATCCACT
    GGTACATCTA AAGAACCAAA TTCTGAGATT ACAGGACATT TCCCTCTGAT ACAACCACAA
    GGAAGTTTAA AAATTCCTGC TAAACTTGAG TGCTTAATTG AACAGGAAGA ACACACTCAG
    GTGAAGAGCA CAGAAAGTTT ATACTGCCAA CATCAATCAA AAAATACCAT GAGAAATAAA
    AGCCGGTGGG CCATGTATTT ATCCTCACAG AGTTCACCTA CACATTCTTC TACAGTAGAT
    GGAAATAATA CAGGAAAAAA TCCTGAGGCC GAGGGAGGTA ATATAAATTT AAATTTGAAA
    GACGCTTTGG TGCAAAAAAG CATGCGGTTT TTAGAAACAT GTACTGAAGA GGGAAAAAAG
    TATAATGAAG ACAAGCCAGT AGTTGATAAT GATCAATCCT GGAATCAAAA AGTAAAATTA
    GAATTTCCTT CATTCTGTGA AAGCAGTGCC TTACCAGTTA CTTGTGACAA CGTAGAAAAC
    GACAGCTTAT TATCAGAAAA TGACAGCTTA TTATCAGAAA ATGACATTCA AGAAAATAAT
    AAAAGACTTT TTAATCAAAA TGACCAGATG GGCATAAAAG GATCAATTCT CATTACAGAA
    AAAGCTCAGG AGATAACCAT GTATGGAACC CCAGCTAAGG AGTATAAACA TCTGCAGATT
    GAATCTTCAC TAAGTAACGA TTTTAGGGTC TCTAATGGCA TTGCTGACAT GTTTTCTAAA
    AGTAATGCTG ATAATGAAAA TCTGAACAGT ATTCATGAAC CACTTGTGGA GATTACTTTT
    AATCTGAACA ATTTTGAAAC CAGTGACTCT GAGGAGGAAT CACAGGAAAG CAGCAGAATT
    TCCCACGATT CAGAGGGTTG GGTGAAGGAA ACTTTAGTTA AAGACAATTC AAGCTGTGAG
    AATATAAACT GTGCAGAAGT TGGCAGTGAC CATTTGCCTC TCTTAACGTC TAGTGGGGAC
    AGACTTGCAG AGACATTTTC CATGAAAGAG ACTCTGCTAT CACAATTTTG TGATAAAACT
    TCTGTAGGTT TTGACACAGG ACCTTGGAAA GCTGGAAACA CTAGAAAAGA AATAGAGGAG
    GAGTACGGTG ACACCTCAAA CAATCTTGAC TCATCTTTAG AGTGGACTGA AGATGCAAAT
    GTAGGTAGTA ATGAAGGCAC TAATAAATCT ACTCAGGAAG TCACAGTTAA CTATGATTTT
    GCTTCTCTCC CAAATGAAAG TATAAACACA AATTTACATA TCCCTCACTC TTTAAACACA
    GCCACTAATC AGGATCCTGA AAACAATCCA TGGTCACAAC AGGCTCAGCC TCAGCCTTTT
    ATTATGGGAA CTGACTTACA CAAAAATACT GAACAGGTTT TACCATTAAC TTCTAGCAGT
    GGCAACGGCA CCCAACTATT AAATGCCAGT CAGAATCACT CTGAAGAATT TCTTGCACTT
    GATAAATCAC ATACCCAAGT TTCCAATTCT TCGTTTTACC CTCTGGGAGT AAAATATCCT
    ATATCTAAAG ACACAGAATC ACATATTCTG GAATCTGAAG ATTTGGGAAG GACTAGAAGT
    TTACCTCATG ACAATACTGA CATAGAAACT GCCAGAGAGA GCAAACAGTA CTGGAATACT
    CCTAAAAATT CTTCAGAACT TTCTGGATTA GAAAATAGCA TTTCCCTATT AAAATCATTG
    TCTGAACACA GTACTGCTTT AGAAGGCTTG GAAATATTGA AAAAGAAACA TACCTCCTTT
    AAGCAACAAG GAACTCTGCA GACGTATGAT CCAGAAAGCA GTCCTGAAGC TAGGAAACCA
    TTGACTACAA TAGTTTCACA AGCTATACCA AAGTCTCATC CGAACCAGGA TCCTCAACAG
    ATGATAAAAG AAAATGAAGT TAAACCAAGT GAATCCCTCA CCAAGTTTTT TCCCTTGGGC
    AGTGAAGAAG AGACTGACTC CCGAGCTCTC ATTCCTAAAC AAATGGAGAG AAAAACCTGT
    GACCCTAAGC CTGTTGAGTT TCAAGGGCAC CAAGTGAAAG GATCAGCTGC TAGTGCTGTG
    ATGGTCAGAG GACACAGCTC CCAGTTTGAA TGCAGTCAAT TTCCAGATAG CGTGTATGAA
    AACTGCATGA CAAATACTTG TTTTTTGACA CCTAAGTTGC CTAGCACTTG TATGCAGATT
    GACTTCTTGC AGTTGCCAGA TGAAGAAAAC TTGATAAAAG GAGATTCCAG CTTTAACACT
    GAAGACAATT TTCAAGTGAT AGCTGAGCCT AATAAGAATT CAGTTAAAAA TGATCTAATG
    AAAAAGTCTC TGGAGAATAA GAACCTTCAA AGGCTTTCAT TAGTAAGTAG AATCCGAGTT
    CCACTTATTA CTTTGCCAGC TACCAGTGGG TCACCTGACC TAGATTCTTG TTCGTGTGTG
    ATCGACTGTG ACAGGCAAGA GTCTTCTGAT TCTCCCATAG TCAACTTGTA TGAAGAGTCA
    GAGCTTCCTT CTTTCAGCTT TGGATCTGAG GACCAAAGTG AAACTTCTTT CTCTAAAGAA
    TCTACTGAAA TGACTCCAAG GGATATTTCA CTTCTCGATA ATACATCTAC TCAAAGCAAG
    TGGCTGAAAT ATCAAAACAT AATCCAATGC AAGTTGACTA CACCAAACAG ACTTGATCAG
    AAAGTAACAG ATGGCTTCTT TGCTGAGGCT GTTTCCAGGA TGCATTTTAG TGACACAGGT
    GAAAGACAGA GTGATGCTGT GAATGAAAGC TTAGACTCTG AGCATTTGCA AATGATAAAA
    TGCATGCTTC AACAACAGCG GCAGGATTTT AGCAGTCAAG ATTTGGTTTC CAGACAGAAA
    GCACTCTCTT TGAATTTAAA GCACACTTCT AAGACTGAGG AAATTAAAAA TGTGTTAGGA
    GAGTCTGCCT GCTACACCTG CAGTGTAAGA GGAGATTTAC AGGAGATAAG TGACTGTGAG
    CTGTGCTTCC CAAGTGGGGA GAAAGTAAAA TCTGCATATC TTCCCCAAAG GCAAATTCAC
    ATACCAGCTG TTTTTCAGTC TCCTGCTCAT TATAAACAGA TTTTTACATC TTGTCTCATA
    GAACATCTAA ATATATTGCT GTTTGGGTTG GCACAAAGGC TGCACAAAGC TCTTTCAAAA
    GTTGACATAT CATTTTACTC ATCAAACGGA GACAAACGGA AAAATGTAGA AAATAATGTG
    CCATCATGCC ATCATCATCA CCCTGCAAAA CTTGTCATGG TTAAAAAGGA AGGTCCAAAT
    AAGGGTCGTC TCTTTTATGC TTGTGATGGA CCCAAAGCTG ACCAATGTAA ATTTTTCAAG
    TGGCTTGATG AAGTGACCCC AGGATATTTA ACACAGGAAG AATCACTACC TAGCATGGTT
    TTAAGTGATA TAAAAAGTAT TGGCTTGTAC TTAAGAAGTC AAAAGATACC CCTCTATGAG
    GAATGCCAAC TTTTGGTGAG AAAAGGATTT GATTTTCAAA GAAAACGGTA TGGTAAACTA
    AAGAAGTTTA CAACTGTAGA TCCTGAATTT TATAACGAAC CTAAGAGCAG ACTTTATCTT
    AAGCTGAGTC GTAAGGAAAG TTCATCTGCT TATAGTAAAG ATGATCTTTG GGTGGTTTCA
    AAAACCCTAG ACTTTGAATT GGATACTTTT ATTGCATGTA GTACATTCTT TGGACCGTCA
    TCTGTCAATG AGGTAGAGCT ACTACCTTTG AAAGGTTATT TCCCTTCCAA CTGGCCCACT
    AACACATTGG TCCATGCATT ATTGGTTTGT AATGCTAGCA CGGAGCTGAC CACTTTGAAA
    AATATTCAGG ACTACTTTAA TCCAGCTACT CTGCCTCTAA CACAGAACCT GTTAACAATG
    TCTTCATCAA CTATAGTGAG CAACAAGAGA GTCAATAAGA GAAAATTTAT CCCACCAGCC
    TTCTCAAATA TCAATACAAA ATTTGAACTG CTCAGTATAG GAGCAACGCT GCAGTTAGCT
    GGTGAGTTAA TTCACACACA CAAGTTAAAC AAGGATCAAG CTACAGCCCT AATTCAGATA
    GCGCAAATGA TGGCATCACA AGGAAGTGCT GAAGAAGTGA AGGAACTGGG AACTCACACC
    CTCCCAATCA CGATCATACA TGGTGTTTTT GGAGCAGGAA AAAGTTATTT GCTGGCAGTG
    GTGATTTTGT TTTTTGTTCA GCTATTTGAA AAGAGTGAAG CTCTTGCAGT TGGAAATGGA
    AGACCTTGGA AACTTCTGAT TTCTTCTTCT ACTAATGTAG CTGTTGACAG AGTACTTCTT
    GGGCTTCTTA GTCTTGGGTT TGAAAAGTTT GTCAGAGTTG GGAGTGTCAG GAAGATTGCC
    AAGCCAATTT TACCTTACAG TTTGCATGCT GGCTCAGAAA ATGAAAATGA ACAATTAAAA
    GAACTGCATA CACTCATGAA GGAAGACCTG ACTCCTGTGG AAAGAGTCTA TGTGAGAAAA
    AGTATTGAGC AACATAAACT GGGGACCAAT AAAACCCTGC TGAAGCAGGT TCGAGTAGTT
    GGAGTTACCT GTGCAGCCTG CCCATTTCCA TGCATGAATG ACCTTAAATT TCCTGTGGTT
    GTACTGGATG AGTGTAGCCA AATAACCGAA CCAGCTTCTC TCCTTCCCAT TGCAAGGTTT
    GAGTGTGAAA AGCTTATTCT TGTTGGAGAT CCCAAACAGC TTCCTCCTAC TATTCAGGGT
    TCTGATGCAG CTCATGAAAA TGGACTGGAA CAAACTCTTT TTGATCGACT TTGCTTAATG
    GGTCACAAGC CAGTTCTTTT GCGAACCCAG TACCGTTGTC ACCCTACAAT CAGTGCTATT
    GCTAATGATC TGTTTTATGA AGGAAACCTC GTGAATGGAG TTTCAGAAAC AGAGAGGAGC
    CCTTTATTGG AATGGCTTCC AACCCTGTGT TTCTATAACA TTCAAGGGCT GGAACAGATC
    GAAAGAGATA ACAGCTTTCA TAACGTAGCA GAAGCTGCTT TTACACTCAA GCTGATTCAA
    TCACTGATTG TGAGTGGAAT AGCAGGCTCT ATGATTGGGG TGATAACATT ATACAAATCC
    CAGATGTACA AGCTTTGTCA CTTACTCACT GCACTGGACT TTGACCATCC TGGTAATGTG
    AAAGCTGTGC AGGTGTCCAC AGTAGATGCT TTTCAGGGCG CTGAAAAGGA GATCATCATT
    CTGTCCTGTG TGAGGACAAG ACAAGTAGGA TTTATTGATT CAGAACAAAG AATGAATGTT
    GCATTGACCA GAGGAAGGAG ACATTTGTTG ATTGTGGGAA ATTTGGCCTG TTTGAGGAAA
    AATCGACTAT GGGGGCGTGT GATCCAACAC TGTGAAGGCA GGGAAGATGG ATTGCAACAT
    GCAAGCCAAT ATGAACCACA GCTCAACCAT CTCCTTAAAG ATTATTTTGA AAAACAAGCA
    GAAGAAAAAC AAAAGAAAAA GAATGAAAAA GATAAATCCA AAGATAAATC TCATTTACAA
    AAAGAAACAG TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020957321.1
    CDS491..6745
    Translation

    Target ORF information:

    RefSeq Version XM_021101662.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa zinc finger GRF-type containing 1 (ZGRF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021101662.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGAAAGCC AGGAATTTAT TGTCCTATAT ACTCATCAAA AGATGAAGAA GTCAAAAGTA 
    TGGCAAGATG GAATTCTGAA GATCACTCAC GTAGGAAACA AAGCCATTTT ATATGATGAC
    AAAGGACAGT GTTTGGAGAG TTTATTTCTT AAGTGCCTTG AGGTGAAACC TGGAGATGAT
    TTAGAAAGTG ATCGATACTT AATCACAGTT GAGGAGGTTA AAGTTGCTGG AAGCATAGCT
    GTTAAACAAG ATATCATTAA AGGAGCACCT CCATTAAATT CAAAGAGCTT TATACCTTCT
    GGCCGGTCCC TTGGGTGTCA ACCTGCTGGT TTAAAAAGGA AATTTACTGG TTTTCAAGGA
    CCACGGCAAG TGCCAAAGAA AATGGCTATT ACAGAAAATA GTGAATCAGC TGCTTCACTT
    GAGGCTAAGG AAGCTGGTCC TGTTTTTCTT TCTCCAGTCT ACAGCACACC TCCTTTGTTT
    CCTACTGTTG GCAAAAAAGA TATAAATTAT ATGCCAACAG ACCCTGACAA CCAGGACAGA
    GAGAGAAATG GCATAGCTTT TTCTTCGGTT GTCTCTACTC CATCCTTCAA GATTAACCCA
    GAAATGCTAT GTGAAGAAAA TTATTTTTGC TCTACTGTCA ATTCTGTAAA TAAGCATTCA
    GGTTCTTTAC TGACCGATGA GCTTATGAAA AGAGATTGTT TGGTATCTCA CAATTCAGGA
    GTTTCACAGA ACATTAGAAG TAAAGCCCAG ATATTGGCTC TTCTGAAATC CAAATCCACT
    GGTACATCTA AAGAACCAAA TTCTGAGATT ACAGGACATT TCCCTCTGAT ACAACCACAA
    GGAAGTTTAA AAATTCCTGC TAAACTTGAG TGCTTAATTG AACAGGAAGA ACACACTCAG
    GTGAAGAGCA CAGAAAGTTT ATACTGCCAA CATCAATCAA AAAATACCAT GAGAAATAAA
    AGCCGGTGGG CCATGTATTT ATCCTCACAG AGTTCACCTA CACATTCTTC TACAGTAGAT
    GGAAATAATA CAGGAAAAAA TCCTGAGGCC GAGGGAGGTA ATATAAATTT AAATTTGAAA
    GACGCTTTGG TGCAAAAAAG CATGCGGTTT TTAGAAACAT GTACTGAAGA GGGAAAAAAG
    TATAATGAAG ACAAGCCAGT AGTTGATAAT GATCAATCCT GGAATCAAAA AGTAAAATTA
    GAATTTCCTT CATTCTGTGA AAGCAGTGCC TTACCAGTTA CTTGTGACAA CGTAGAAAAC
    GACAGCTTAT TATCAGAAAA TGACAGCTTA TTATCAGAAA ATGACATTCA AGAAAATAAT
    AAAAGACTTT TTAATCAAAA TGACCAGATG GGCATAAAAG GATCAATTCT CATTACAGAA
    AAAGCTCAGG AGATAACCAT GTATGGAACC CCAGCTAAGG AGTATAAACA TCTGCAGATT
    GAATCTTCAC TAAGTAACGA TTTTAGGGTC TCTAATGGCA TTGCTGACAT GTTTTCTAAA
    AGTAATGCTG ATAATGAAAA TCTGAACAGT ATTCATGAAC CACTTGTGGA GATTACTTTT
    AATCTGAACA ATTTTGAAAC CAGTGACTCT GAGGAGGAAT CACAGGAAAG CAGCAGAATT
    TCCCACGATT CAGAGGGTTG GGTGAAGGAA ACTTTAGTTA AAGACAATTC AAGCTGTGAG
    AATATAAACT GTGCAGAAGT TGGCAGTGAC CATTTGCCTC TCTTAACGTC TAGTGGGGAC
    AGACTTGCAG AGACATTTTC CATGAAAGAG ACTCTGCTAT CACAATTTTG TGATAAAACT
    TCTGTAGGTT TTGACACAGG ACCTTGGAAA GCTGGAAACA CTAGAAAAGA AATAGAGGAG
    GAGTACGGTG ACACCTCAAA CAATCTTGAC TCATCTTTAG AGTGGACTGA AGATGCAAAT
    GTAGGTAGTA ATGAAGGCAC TAATAAATCT ACTCAGGAAG TCACAGTTAA CTATGATTTT
    GCTTCTCTCC CAAATGAAAG TATAAACACA AATTTACATA TCCCTCACTC TTTAAACACA
    GCCACTAATC AGGATCCTGA AAACAATCCA TGGTCACAAC AGGCTCAGCC TCAGCCTTTT
    ATTATGGGAA CTGACTTACA CAAAAATACT GAACAGGTTT TACCATTAAC TTCTAGCAGT
    GGCAACGGCA CCCAACTATT AAATGCCAGT CAGAATCACT CTGAAGAATT TCTTGCACTT
    GATAAATCAC ATACCCAAGT TTCCAATTCT TCGTTTTACC CTCTGGGAGT AAAATATCCT
    ATATCTAAAG ACACAGAATC ACATATTCTG GAATCTGAAG ATTTGGGAAG GACTAGAAGT
    TTACCTCATG ACAATACTGA CATAGAAACT GCCAGAGAGA GCAAACAGTA CTGGAATACT
    CCTAAAAATT CTTCAGAACT TTCTGGATTA GAAAATAGCA TTTCCCTATT AAAATCATTG
    TCTGAACACA GTACTGCTTT AGAAGGCTTG GAAATATTGA AAAAGAAACA TACCTCCTTT
    AAGCAACAAG GAACTCTGCA GACGTATGAT CCAGAAAGCA GTCCTGAAGC TAGGAAACCA
    TTGACTACAA TAGTTTCACA AGCTATACCA AAGTCTCATC CGAACCAGGA TCCTCAACAG
    ATGATAAAAG AAAATGAAGT TAAACCAAGT GAATCCCTCA CCAAGTTTTT TCCCTTGGGC
    AGTGAAGAAG AGACTGACTC CCGAGCTCTC ATTCCTAAAC AAATGGAGAG AAAAACCTGT
    GACCCTAAGC CTGTTGAGTT TCAAGGGCAC CAAGTGAAAG GATCAGCTGC TAGTGCTGTG
    ATGGTCAGAG GACACAGCTC CCAGTTTGAA TGCAGTCAAT TTCCAGATAG CGTGTATGAA
    AACTGCATGA CAAATACTTG TTTTTTGACA CCTAAGTTGC CTAGCACTTG TATGCAGATT
    GACTTCTTGC AGTTGCCAGA TGAAGAAAAC TTGATAAAAG GAGATTCCAG CTTTAACACT
    GAAGACAATT TTCAAGTGAT AGCTGAGCCT AATAAGAATT CAGTTAAAAA TGATCTAATG
    AAAAAGTCTC TGGAGAATAA GAACCTTCAA AGGCTTTCAT TAGTAAGTAG AATCCGAGTT
    CCACTTATTA CTTTGCCAGC TACCAGTGGG TCACCTGACC TAGATTCTTG TTCGTGTGTG
    ATCGACTGTG ACAGGCAAGA GTCTTCTGAT TCTCCCATAG TCAACTTGTA TGAAGAGTCA
    GAGCTTCCTT CTTTCAGCTT TGGATCTGAG GACCAAAGTG AAACTTCTTT CTCTAAAGAA
    TCTACTGAAA TGACTCCAAG GGATATTTCA CTTCTCGATA ATACATCTAC TCAAAGCAAG
    TGGCTGAAAT ATCAAAACAT AATCCAATGC AAGTTGACTA CACCAAACAG ACTTGATCAG
    AAAGTAACAG ATGGCTTCTT TGCTGAGGCT GTTTCCAGGA TGCATTTTAG TGACACAGGT
    GAAAGACAGA GTGATGCTGT GAATGAAAGC TTAGACTCTG AGCATTTGCA AATGATAAAA
    TGCATGCTTC AACAACAGCG GCAGGATTTT AGCAGTCAAG ATTTGGTTTC CAGACAGAAA
    GCACTCTCTT TGAATTTAAA GCACACTTCT AAGACTGAGG AAATTAAAAA TGTGTTAGGA
    GAGTCTGCCT GCTACACCTG CAGTGTAAGA GGAGATTTAC AGGAGATAAG TGACTGTGAG
    CTGTGCTTCC CAAGTGGGGA GAAAGTAAAA TCTGCATATC TTCCCCAAAG GCAAATTCAC
    ATACCAGCTG TTTTTCAGTC TCCTGCTCAT TATAAACAGA TTTTTACATC TTGTCTCATA
    GAACATCTAA ATATATTGCT GTTTGGGTTG GCACAAAGGC TGCACAAAGC TCTTTCAAAA
    GTTGACATAT CATTTTACTC ATCAAACGGA GACAAACGGA AAAATGTAGA AAATAATGTG
    CCATCATGCC ATCATCATCA CCCTGCAAAA CTTGTCATGG TTAAAAAGGA AGGTCCAAAT
    AAGGGTCGTC TCTTTTATGC TTGTGATGGA CCCAAAGCTG ACCAATGTAA ATTTTTCAAG
    TGGCTTGATG AAGTGACCCC AGGATATTTA ACACAGGAAG AATCACTACC TAGCATGGTT
    TTAAGTGATA TAAAAAGTAT TGGCTTGTAC TTAAGAAGTC AAAAGATACC CCTCTATGAG
    GAATGCCAAC TTTTGGTGAG AAAAGGATTT GATTTTCAAA GAAAACGGTA TGGTAAACTA
    AAGAAGTTTA CAACTGTAGA TCCTGAATTT TATAACGAAC CTAAGAGCAG ACTTTATCTT
    AAGCTGAGTC GTAAGGAAAG TTCATCTGCT TATAGTAAAG ATGATCTTTG GGTGGTTTCA
    AAAACCCTAG ACTTTGAATT GGATACTTTT ATTGCATGTA GTACATTCTT TGGACCGTCA
    TCTGTCAATG AGGTAGAGCT ACTACCTTTG AAAGGTTATT TCCCTTCCAA CTGGCCCACT
    AACACATTGG TCCATGCATT ATTGGTTTGT AATGCTAGCA CGGAGCTGAC CACTTTGAAA
    AATATTCAGG ACTACTTTAA TCCAGCTACT CTGCCTCTAA CACAGAACCT GTTAACAATG
    TCTTCATCAA CTATAGTGAG CAACAAGAGA GTCAATAAGA GAAAATTTAT CCCACCAGCC
    TTCTCAAATA TCAATACAAA ATTTGAACTG CTCAGTATAG GAGCAACGCT GCAGTTAGCT
    GGTGAGTTAA TTCACACACA CAAGTTAAAC AAGGATCAAG CTACAGCCCT AATTCAGATA
    GCGCAAATGA TGGCATCACA AGGAAGTGCT GAAGAAGTGA AGGAACTGGG AACTCACACC
    CTCCCAATCA CGATCATACA TGGTGTTTTT GGAGCAGGAA AAAGTTATTT GCTGGCAGTG
    GTGATTTTGT TTTTTGTTCA GCTATTTGAA AAGAGTGAAG CTCTTGCAGT TGGAAATGGA
    AGACCTTGGA AACTTCTGAT TTCTTCTTCT ACTAATGTAG CTGTTGACAG AGTACTTCTT
    GGGCTTCTTA GTCTTGGGTT TGAAAAGTTT GTCAGAGTTG GGAGTGTCAG GAAGATTGCC
    AAGCCAATTT TACCTTACAG TTTGCATGCT GGCTCAGAAA ATGAAAATGA ACAATTAAAA
    GAACTGCATA CACTCATGAA GGAAGACCTG ACTCCTGTGG AAAGAGTCTA TGTGAGAAAA
    AGTATTGAGC AACATAAACT GGGGACCAAT AAAACCCTGC TGAAGCAGGT TCGAGTAGTT
    GGAGTTACCT GTGCAGCCTG CCCATTTCCA TGCATGAATG ACCTTAAATT TCCTGTGGTT
    GTACTGGATG AGTGTAGCCA AATAACCGAA CCAGCTTCTC TCCTTCCCAT TGCAAGGTTT
    GAGTGTGAAA AGCTTATTCT TGTTGGAGAT CCCAAACAGC TTCCTCCTAC TATTCAGGGT
    TCTGATGCAG CTCATGAAAA TGGACTGGAA CAAACTCTTT TTGATCGACT TTGCTTAATG
    GGTCACAAGC CAGTTCTTTT GCGAACCCAG TACCGTTGTC ACCCTACAAT CAGTGCTATT
    GCTAATGATC TGTTTTATGA AGGAAACCTC GTGAATGGAG TTTCAGAAAC AGAGAGGAGC
    CCTTTATTGG AATGGCTTCC AACCCTGTGT TTCTATAACA TTCAAGGGCT GGAACAGATC
    GAAAGAGATA ACAGCTTTCA TAACGTAGCA GAAGCTGCTT TTACACTCAA GCTGATTCAA
    TCACTGATTG TGAGTGGAAT AGCAGGCTCT ATGATTGGGG TGATAACATT ATACAAATCC
    CAGATGTACA AGCTTTGTCA CTTACTCACT GCACTGGACT TTGACCATCC TGGTAATGTG
    AAAGCTGTGC AGGTGTCCAC AGTAGATGCT TTTCAGGGCG CTGAAAAGGA GATCATCATT
    CTGTCCTGTG TGAGGACAAG ACAAGTAGGA TTTATTGATT CAGAACAAAG AATGAATGTT
    GCATTGACCA GAGGAAGGAG ACATTTGTTG ATTGTGGGAA ATTTGGCCTG TTTGAGGAAA
    AATCGACTAT GGGGGCGTGT GATCCAACAC TGTGAAGGCA GGGAAGATGG ATTGCAACAT
    GCAAGCCAAT ATGAACCACA GCTCAACCAT CTCCTTAAAG ATTATTTTGA AAAACAAGCA
    GAAGAAAAAC AAAAGAAAAA GAATGAAAAA GATAAATCCA AAGATAAATC TCATTTACAA
    AAAGAAACAG TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSe224827
    Clone ID Related Accession (Same CDS sequence) XM_021101664.1
    Accession Version XM_021101664.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6072bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-12
    Organism Sus scrofa(pig)
    Product protein ZGRF1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010450.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGAAAGCC AGGAATTTAT TGTCCTATAT ACTCATCAAA AGATGAAGAA GTCAAAAGTA 
    TGGCAAGATG GAATTCTGAA GATCACTCAC GTAGGAAACA AAGCCATTTT ATATGATGAC
    AAAGGACAGT GTTTGGAGAG TTTATTTCTT AAGTGCCTTG AGGTGAAACC TGGAGATGAT
    TTAGAAAGTG ATCGATACTT AATCACAGTT GAGGAGGTTA AAGTTGCTGG AAGCATAGCT
    GTTAAACAAG ATATCATTAA AGGAGCACCT CCATTAAATT CAAAGAGCTT TATACCTTCT
    GGCCGGTCCC TTGGGTGTCA ACCTGCTGGT TTAAAAAGGA AATTTACTGG TTTTCAAGGA
    CCACGGCAAG TGCCAAAGAA AATGGCTATT ACAGAAAATA GTGAATCAGC TGCTTCACTT
    GAGGCTAAGG AAGCTGGTCC TGTTTTTCTT TCTCCAGTCT ACAGCACACC TCCTTTGTTT
    CCTACTGTTG GCAAAAAAGA TATAAATTAT ATGCCAACAG ACCCTGACAA CCAGGACAGA
    GAGAGAAATG GCATAGCTTT TTCTTCGGTT GTCTCTACTC CATCCTTCAA GATTAACCCA
    GAAATGCTAT GTGAAGAAAA TTATTTTTGC TCTACTGTCA ATTCTGTAAA TAAGCATTCA
    GGTTCTTTAC TGACCGATGA GCTTATGAAA AGAGATTGTT TGGTATCTCA CAATTCAGGA
    GTTTCACAGA ACATTAGAAG TAAAGCCCAG ATATTGGCTC TTCTGAAATC CAAATCCACT
    GGTACATCTA AAGAACCAAA TTCTGAGATT ACAGGACATT TCCCTCTGAT ACAACCACAA
    GGAAGTTTAA AAATTCCTGC TAAACTTGAG TGCTTAATTG AACAGGAAGA ACACACTCAG
    GTGAAGAGCA CAGAAAGTTT ATACTGCCAA CATCAATCAA AAAATACCAT GAGAAATAAA
    AGCCGGTGGG CCATGTATTT ATCCTCACAG AGTTCACCTA CACATTCTTC TACAGTAGAT
    GGAAATAATA CAGGAAAAAA TCCTGAGGCC GAGGGAGGTA ATATAAATTT AAATTTGAAA
    GACGCTTTGG TGCAAAAAAG CATGCGGTTT TTAGAAACAT GTACTGAAGA GGGAAAAAAG
    TATAATGAAG ACAAGCCAGT AGTTGATAAT GATCAATCCT GGAATCAAAA AGTAAAATTA
    GAATTTCCTT CATTCTGTGA AAGCAGTGCC TTACCAGTTA CTTGTGACAA CGTAGAAAAC
    GACAGCTTAT TATCAGAAAA TGACAGCTTA TTATCAGAAA ATGACATTCA AGAAAATAAT
    AAAAGACTTT TTAATCAAAA TGACCAGATG GGCATAAAAG GATCAATTCT CATTACAGAA
    AAAGCTCAGG AGATAACCAT GTATGGAACC CCAGCTAAGG AGTATAAACA TCTGCAGATT
    GAATCTTCAC TAAGTAACGA TTTTAGGGTC TCTAATGGCA TTGCTGACAT GTTTTCTAAA
    AGTAATGCTG ATAATGAAAA TCTGAACAGT ATTCATGAAC CACTTGTGGA GATTACTTTT
    AATCTGAACA ATTTTGAAAC CAGTGACTCT GAGGAGGAAT CACAGGAAAG CAGCAGAATT
    TCCCACGATT CAGAGGGTTG GGTGAAGGAA ACTTTAGTTA AAGACAATTC AAGCTGTGAG
    AATATAAACT GTGCAGAAGT TGGCAGTGAC CATTTGCCTC TCTTAACGTC TAGTGGGGAC
    AGACTTGCAG AGACATTTTC CATGAAAGAG ACTCTGCTAT CACAATTTTG TGATAAAACT
    TCTGTAGGTT TTGACACAGG ACCTTGGAAA GCTGGAAACA CTAGAAAAGA AATAGAGGAG
    GAGTACGGTG ACACCTCAAA CAATCTTGAC TCATCTTTAG AGTGGACTGA AGATGCAAAT
    GTAGGTAGTA ATGAAGGCAC TAATAAATCT ACTCAGGAAG TCACAGTTAA CTATGATTTT
    GCTTCTCTCC CAAATGAAAG TATAAACACA AATTTACATA TCCCTCACTC TTTAAACACA
    GCCACTAATC AGGATCCTGA AAACAATCCA TGGTCACAAC AGGCTCAGCC TCAGCCTTTT
    ATTATGGGAA CTGACTTACA CAAAAATACT GAACAGGTTT TACCATTAAC TTCTAGCAGT
    GGCAACGGCA CCCAACTATT AAATGCCAGT CAGAATCACT CTGAAGAATT TCTTGCACTT
    GATAAATCAC ATACCCAAGT TTCCAATTCT TCGTTTTACC CTCTGGGAGT AAAATATCCT
    ATATCTAAAG ACACAGAATC ACATATTCTG GAATCTGAAG ATTTGGGAAG GACTAGAAGT
    TTACCTCATG ACAATACTGA CATAGAAACT GCCAGAGAGA GCAAACAGTA CTGGAATACT
    CCTAAAAATT CTTCAGAACT TTCTGGATTA GAAAATAGCA TTTCCCTATT AAAATCATTG
    TCTGAACACA GTACTGCTTT AGAAGGCTTG GAAATATTGA AAAAGAAACA TACCTCCTTT
    AAGCAACAAG GAACTCTGCA GACGTATGAT CCAGAAAGCA GTCCTGAAGC TAGGAAACCA
    TTGACTACAA TAGTTTCACA AGCTATACCA AAGTCTCATC CGAACCAGGA TCCTCAACAG
    ATGATAAAAG AAAATGAAGT TAAACCAAGT GAATCCCTCA CCAAGTTTTT TCCCTTGGGC
    AGTGAAGAAG AGACTGACTC CCGAGCTCTC ATTCCTAAAC AAATGGAGAG AAAAACCTGT
    GACCCTAAGT TGCCAGATGA AGAAAACTTG ATAAAAGGAG ATTCCAGCTT TAACACTGAA
    GACAATTTTC AAGTGATAGC TGAGCCTAAT AAGAATTCAG TTAAAAATGA TCTAATGAAA
    AAGTCTCTGG AGAATAAGAA CCTTCAAAGG CTTTCATTAG TAAGTAGAAT CCGAGTTCCA
    CTTATTACTT TGCCAGCTAC CAGTGGGTCA CCTGACCTAG ATTCTTGTTC GTGTGTGATC
    GACTGTGACA GGCAAGAGTC TTCTGATTCT CCCATAGTCA ACTTGTATGA AGAGTCAGAG
    CTTCCTTCTT TCAGCTTTGG ATCTGAGGAC CAAAGTGAAA CTTCTTTCTC TAAAGAATCT
    ACTGAAATGA CTCCAAGGGA TATTTCACTT CTCGATAATA CATCTACTCA AAGCAAGTGG
    CTGAAATATC AAAACATAAT CCAATGCAAG TTGACTACAC CAAACAGACT TGATCAGAAA
    GTAACAGATG GCTTCTTTGC TGAGGCTGTT TCCAGGATGC ATTTTAGTGA CACAGGTGAA
    AGACAGAGTG ATGCTGTGAA TGAAAGCTTA GACTCTGAGC ATTTGCAAAT GATAAAATGC
    ATGCTTCAAC AACAGCGGCA GGATTTTAGC AGTCAAGATT TGGTTTCCAG ACAGAAAGCA
    CTCTCTTTGA ATTTAAAGCA CACTTCTAAG ACTGAGGAAA TTAAAAATGT GTTAGGAGAG
    TCTGCCTGCT ACACCTGCAG TGTAAGAGGA GATTTACAGG AGATAAGTGA CTGTGAGCTG
    TGCTTCCCAA GTGGGGAGAA AGTAAAATCT GCATATCTTC CCCAAAGGCA AATTCACATA
    CCAGCTGTTT TTCAGTCTCC TGCTCATTAT AAACAGATTT TTACATCTTG TCTCATAGAA
    CATCTAAATA TATTGCTGTT TGGGTTGGCA CAAAGGCTGC ACAAAGCTCT TTCAAAAGTT
    GACATATCAT TTTACTCATC AAACGGAGAC AAACGGAAAA ATGTAGAAAA TAATGTGCCA
    TCATGCCATC ATCATCACCC TGCAAAACTT GTCATGGTTA AAAAGGAAGG TCCAAATAAG
    GGTCGTCTCT TTTATGCTTG TGATGGACCC AAAGCTGACC AATGTAAATT TTTCAAGTGG
    CTTGATGAAG TGACCCCAGG ATATTTAACA CAGGAAGAAT CACTACCTAG CATGGTTTTA
    AGTGATATAA AAAGTATTGG CTTGTACTTA AGAAGTCAAA AGATACCCCT CTATGAGGAA
    TGCCAACTTT TGGTGAGAAA AGGATTTGAT TTTCAAAGAA AACGGTATGG TAAACTAAAG
    AAGTTTACAA CTGTAGATCC TGAATTTTAT AACGAACCTA AGAGCAGACT TTATCTTAAG
    CTGAGTCGTA AGGAAAGTTC ATCTGCTTAT AGTAAAGATG ATCTTTGGGT GGTTTCAAAA
    ACCCTAGACT TTGAATTGGA TACTTTTATT GCATGTAGTA CATTCTTTGG ACCGTCATCT
    GTCAATGAGG TAGAGCTACT ACCTTTGAAA GGTTATTTCC CTTCCAACTG GCCCACTAAC
    ACATTGGTCC ATGCATTATT GGTTTGTAAT GCTAGCACGG AGCTGACCAC TTTGAAAAAT
    ATTCAGGACT ACTTTAATCC AGCTACTCTG CCTCTAACAC AGAACCTGTT AACAATGTCT
    TCATCAACTA TAGTGAGCAA CAAGAGAGTC AATAAGAGAA AATTTATCCC ACCAGCCTTC
    TCAAATATCA ATACAAAATT TGAACTGCTC AGTATAGGAG CAACGCTGCA GTTAGCTGGT
    GAGTTAATTC ACACACACAA GTTAAACAAG GATCAAGCTA CAGCCCTAAT TCAGATAGCG
    CAAATGATGG CATCACAAGG AAGTGCTGAA GAAGTGAAGG AACTGGGAAC TCACACCCTC
    CCAATCACGA TCATACATGG TGTTTTTGGA GCAGGAAAAA GTTATTTGCT GGCAGTGGTG
    ATTTTGTTTT TTGTTCAGCT ATTTGAAAAG AGTGAAGCTC TTGCAGTTGG AAATGGAAGA
    CCTTGGAAAC TTCTGATTTC TTCTTCTACT AATGTAGCTG TTGACAGAGT ACTTCTTGGG
    CTTCTTAGTC TTGGGTTTGA AAAGTTTGTC AGAGTTGGGA GTGTCAGGAA GATTGCCAAG
    CCAATTTTAC CTTACAGTTT GCATGCTGGC TCAGAAAATG AAAATGAACA ATTAAAAGAA
    CTGCATACAC TCATGAAGGA AGACCTGACT CCTGTGGAAA GAGTCTATGT GAGAAAAAGT
    ATTGAGCAAC ATAAACTGGG GACCAATAAA ACCCTGCTGA AGCAGGTTCG AGTAGTTGGA
    GTTACCTGTG CAGCCTGCCC ATTTCCATGC ATGAATGACC TTAAATTTCC TGTGGTTGTA
    CTGGATGAGT GTAGCCAAAT AACCGAACCA GCTTCTCTCC TTCCCATTGC AAGGTTTGAG
    TGTGAAAAGC TTATTCTTGT TGGAGATCCC AAACAGCTTC CTCCTACTAT TCAGGGTTCT
    GATGCAGCTC ATGAAAATGG ACTGGAACAA ACTCTTTTTG ATCGACTTTG CTTAATGGGT
    CACAAGCCAG TTCTTTTGCG AACCCAGTAC CGTTGTCACC CTACAATCAG TGCTATTGCT
    AATGATCTGT TTTATGAAGG AAACCTCGTG AATGGAGTTT CAGAAACAGA GAGGAGCCCT
    TTATTGGAAT GGCTTCCAAC CCTGTGTTTC TATAACATTC AAGGGCTGGA ACAGATCGAA
    AGAGATAACA GCTTTCATAA CGTAGCAGAA GCTGCTTTTA CACTCAAGCT GATTCAATCA
    CTGATTGTGA GTGGAATAGC AGGCTCTATG ATTGGGGTGA TAACATTATA CAAATCCCAG
    ATGTACAAGC TTTGTCACTT ACTCACTGCA CTGGACTTTG ACCATCCTGG TAATGTGAAA
    GCTGTGCAGG TGTCCACAGT AGATGCTTTT CAGGGCGCTG AAAAGGAGAT CATCATTCTG
    TCCTGTGTGA GGACAAGACA AGTAGGATTT ATTGATTCAG AACAAAGAAT GAATGTTGCA
    TTGACCAGAG GAAGGAGACA TTTGTTGATT GTGGGAAATT TGGCCTGTTT GAGGAAAAAT
    CGACTATGGG GGCGTGTGAT CCAACACTGT GAAGGCAGGG AAGATGGATT GCAACATGCA
    AGCCAATATG AACCACAGCT CAACCATCTC CTTAAAGATT ATTTTGAAAA ACAAGCAGAA
    GAAAAACAAA AGAAAAAGAA TGAAAAAGAT AAATCCAAAG ATAAATCTCA TTTACAAAAA
    GAAACAGTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_020957323.1
    CDS405..6476
    Translation

    Target ORF information:

    RefSeq Version XM_021101664.1
    Organism Sus scrofa(pig)
    Definition Sus scrofa zinc finger GRF-type containing 1 (ZGRF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021101664.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGAAAGCC AGGAATTTAT TGTCCTATAT ACTCATCAAA AGATGAAGAA GTCAAAAGTA 
    TGGCAAGATG GAATTCTGAA GATCACTCAC GTAGGAAACA AAGCCATTTT ATATGATGAC
    AAAGGACAGT GTTTGGAGAG TTTATTTCTT AAGTGCCTTG AGGTGAAACC TGGAGATGAT
    TTAGAAAGTG ATCGATACTT AATCACAGTT GAGGAGGTTA AAGTTGCTGG AAGCATAGCT
    GTTAAACAAG ATATCATTAA AGGAGCACCT CCATTAAATT CAAAGAGCTT TATACCTTCT
    GGCCGGTCCC TTGGGTGTCA ACCTGCTGGT TTAAAAAGGA AATTTACTGG TTTTCAAGGA
    CCACGGCAAG TGCCAAAGAA AATGGCTATT ACAGAAAATA GTGAATCAGC TGCTTCACTT
    GAGGCTAAGG AAGCTGGTCC TGTTTTTCTT TCTCCAGTCT ACAGCACACC TCCTTTGTTT
    CCTACTGTTG GCAAAAAAGA TATAAATTAT ATGCCAACAG ACCCTGACAA CCAGGACAGA
    GAGAGAAATG GCATAGCTTT TTCTTCGGTT GTCTCTACTC CATCCTTCAA GATTAACCCA
    GAAATGCTAT GTGAAGAAAA TTATTTTTGC TCTACTGTCA ATTCTGTAAA TAAGCATTCA
    GGTTCTTTAC TGACCGATGA GCTTATGAAA AGAGATTGTT TGGTATCTCA CAATTCAGGA
    GTTTCACAGA ACATTAGAAG TAAAGCCCAG ATATTGGCTC TTCTGAAATC CAAATCCACT
    GGTACATCTA AAGAACCAAA TTCTGAGATT ACAGGACATT TCCCTCTGAT ACAACCACAA
    GGAAGTTTAA AAATTCCTGC TAAACTTGAG TGCTTAATTG AACAGGAAGA ACACACTCAG
    GTGAAGAGCA CAGAAAGTTT ATACTGCCAA CATCAATCAA AAAATACCAT GAGAAATAAA
    AGCCGGTGGG CCATGTATTT ATCCTCACAG AGTTCACCTA CACATTCTTC TACAGTAGAT
    GGAAATAATA CAGGAAAAAA TCCTGAGGCC GAGGGAGGTA ATATAAATTT AAATTTGAAA
    GACGCTTTGG TGCAAAAAAG CATGCGGTTT TTAGAAACAT GTACTGAAGA GGGAAAAAAG
    TATAATGAAG ACAAGCCAGT AGTTGATAAT GATCAATCCT GGAATCAAAA AGTAAAATTA
    GAATTTCCTT CATTCTGTGA AAGCAGTGCC TTACCAGTTA CTTGTGACAA CGTAGAAAAC
    GACAGCTTAT TATCAGAAAA TGACAGCTTA TTATCAGAAA ATGACATTCA AGAAAATAAT
    AAAAGACTTT TTAATCAAAA TGACCAGATG GGCATAAAAG GATCAATTCT CATTACAGAA
    AAAGCTCAGG AGATAACCAT GTATGGAACC CCAGCTAAGG AGTATAAACA TCTGCAGATT
    GAATCTTCAC TAAGTAACGA TTTTAGGGTC TCTAATGGCA TTGCTGACAT GTTTTCTAAA
    AGTAATGCTG ATAATGAAAA TCTGAACAGT ATTCATGAAC CACTTGTGGA GATTACTTTT
    AATCTGAACA ATTTTGAAAC CAGTGACTCT GAGGAGGAAT CACAGGAAAG CAGCAGAATT
    TCCCACGATT CAGAGGGTTG GGTGAAGGAA ACTTTAGTTA AAGACAATTC AAGCTGTGAG
    AATATAAACT GTGCAGAAGT TGGCAGTGAC CATTTGCCTC TCTTAACGTC TAGTGGGGAC
    AGACTTGCAG AGACATTTTC CATGAAAGAG ACTCTGCTAT CACAATTTTG TGATAAAACT
    TCTGTAGGTT TTGACACAGG ACCTTGGAAA GCTGGAAACA CTAGAAAAGA AATAGAGGAG
    GAGTACGGTG ACACCTCAAA CAATCTTGAC TCATCTTTAG AGTGGACTGA AGATGCAAAT
    GTAGGTAGTA ATGAAGGCAC TAATAAATCT ACTCAGGAAG TCACAGTTAA CTATGATTTT
    GCTTCTCTCC CAAATGAAAG TATAAACACA AATTTACATA TCCCTCACTC TTTAAACACA
    GCCACTAATC AGGATCCTGA AAACAATCCA TGGTCACAAC AGGCTCAGCC TCAGCCTTTT
    ATTATGGGAA CTGACTTACA CAAAAATACT GAACAGGTTT TACCATTAAC TTCTAGCAGT
    GGCAACGGCA CCCAACTATT AAATGCCAGT CAGAATCACT CTGAAGAATT TCTTGCACTT
    GATAAATCAC ATACCCAAGT TTCCAATTCT TCGTTTTACC CTCTGGGAGT AAAATATCCT
    ATATCTAAAG ACACAGAATC ACATATTCTG GAATCTGAAG ATTTGGGAAG GACTAGAAGT
    TTACCTCATG ACAATACTGA CATAGAAACT GCCAGAGAGA GCAAACAGTA CTGGAATACT
    CCTAAAAATT CTTCAGAACT TTCTGGATTA GAAAATAGCA TTTCCCTATT AAAATCATTG
    TCTGAACACA GTACTGCTTT AGAAGGCTTG GAAATATTGA AAAAGAAACA TACCTCCTTT
    AAGCAACAAG GAACTCTGCA GACGTATGAT CCAGAAAGCA GTCCTGAAGC TAGGAAACCA
    TTGACTACAA TAGTTTCACA AGCTATACCA AAGTCTCATC CGAACCAGGA TCCTCAACAG
    ATGATAAAAG AAAATGAAGT TAAACCAAGT GAATCCCTCA CCAAGTTTTT TCCCTTGGGC
    AGTGAAGAAG AGACTGACTC CCGAGCTCTC ATTCCTAAAC AAATGGAGAG AAAAACCTGT
    GACCCTAAGT TGCCAGATGA AGAAAACTTG ATAAAAGGAG ATTCCAGCTT TAACACTGAA
    GACAATTTTC AAGTGATAGC TGAGCCTAAT AAGAATTCAG TTAAAAATGA TCTAATGAAA
    AAGTCTCTGG AGAATAAGAA CCTTCAAAGG CTTTCATTAG TAAGTAGAAT CCGAGTTCCA
    CTTATTACTT TGCCAGCTAC CAGTGGGTCA CCTGACCTAG ATTCTTGTTC GTGTGTGATC
    GACTGTGACA GGCAAGAGTC TTCTGATTCT CCCATAGTCA ACTTGTATGA AGAGTCAGAG
    CTTCCTTCTT TCAGCTTTGG ATCTGAGGAC CAAAGTGAAA CTTCTTTCTC TAAAGAATCT
    ACTGAAATGA CTCCAAGGGA TATTTCACTT CTCGATAATA CATCTACTCA AAGCAAGTGG
    CTGAAATATC AAAACATAAT CCAATGCAAG TTGACTACAC CAAACAGACT TGATCAGAAA
    GTAACAGATG GCTTCTTTGC TGAGGCTGTT TCCAGGATGC ATTTTAGTGA CACAGGTGAA
    AGACAGAGTG ATGCTGTGAA TGAAAGCTTA GACTCTGAGC ATTTGCAAAT GATAAAATGC
    ATGCTTCAAC AACAGCGGCA GGATTTTAGC AGTCAAGATT TGGTTTCCAG ACAGAAAGCA
    CTCTCTTTGA ATTTAAAGCA CACTTCTAAG ACTGAGGAAA TTAAAAATGT GTTAGGAGAG
    TCTGCCTGCT ACACCTGCAG TGTAAGAGGA GATTTACAGG AGATAAGTGA CTGTGAGCTG
    TGCTTCCCAA GTGGGGAGAA AGTAAAATCT GCATATCTTC CCCAAAGGCA AATTCACATA
    CCAGCTGTTT TTCAGTCTCC TGCTCATTAT AAACAGATTT TTACATCTTG TCTCATAGAA
    CATCTAAATA TATTGCTGTT TGGGTTGGCA CAAAGGCTGC ACAAAGCTCT TTCAAAAGTT
    GACATATCAT TTTACTCATC AAACGGAGAC AAACGGAAAA ATGTAGAAAA TAATGTGCCA
    TCATGCCATC ATCATCACCC TGCAAAACTT GTCATGGTTA AAAAGGAAGG TCCAAATAAG
    GGTCGTCTCT TTTATGCTTG TGATGGACCC AAAGCTGACC AATGTAAATT TTTCAAGTGG
    CTTGATGAAG TGACCCCAGG ATATTTAACA CAGGAAGAAT CACTACCTAG CATGGTTTTA
    AGTGATATAA AAAGTATTGG CTTGTACTTA AGAAGTCAAA AGATACCCCT CTATGAGGAA
    TGCCAACTTT TGGTGAGAAA AGGATTTGAT TTTCAAAGAA AACGGTATGG TAAACTAAAG
    AAGTTTACAA CTGTAGATCC TGAATTTTAT AACGAACCTA AGAGCAGACT TTATCTTAAG
    CTGAGTCGTA AGGAAAGTTC ATCTGCTTAT AGTAAAGATG ATCTTTGGGT GGTTTCAAAA
    ACCCTAGACT TTGAATTGGA TACTTTTATT GCATGTAGTA CATTCTTTGG ACCGTCATCT
    GTCAATGAGG TAGAGCTACT ACCTTTGAAA GGTTATTTCC CTTCCAACTG GCCCACTAAC
    ACATTGGTCC ATGCATTATT GGTTTGTAAT GCTAGCACGG AGCTGACCAC TTTGAAAAAT
    ATTCAGGACT ACTTTAATCC AGCTACTCTG CCTCTAACAC AGAACCTGTT AACAATGTCT
    TCATCAACTA TAGTGAGCAA CAAGAGAGTC AATAAGAGAA AATTTATCCC ACCAGCCTTC
    TCAAATATCA ATACAAAATT TGAACTGCTC AGTATAGGAG CAACGCTGCA GTTAGCTGGT
    GAGTTAATTC ACACACACAA GTTAAACAAG GATCAAGCTA CAGCCCTAAT TCAGATAGCG
    CAAATGATGG CATCACAAGG AAGTGCTGAA GAAGTGAAGG AACTGGGAAC TCACACCCTC
    CCAATCACGA TCATACATGG TGTTTTTGGA GCAGGAAAAA GTTATTTGCT GGCAGTGGTG
    ATTTTGTTTT TTGTTCAGCT ATTTGAAAAG AGTGAAGCTC TTGCAGTTGG AAATGGAAGA
    CCTTGGAAAC TTCTGATTTC TTCTTCTACT AATGTAGCTG TTGACAGAGT ACTTCTTGGG
    CTTCTTAGTC TTGGGTTTGA AAAGTTTGTC AGAGTTGGGA GTGTCAGGAA GATTGCCAAG
    CCAATTTTAC CTTACAGTTT GCATGCTGGC TCAGAAAATG AAAATGAACA ATTAAAAGAA
    CTGCATACAC TCATGAAGGA AGACCTGACT CCTGTGGAAA GAGTCTATGT GAGAAAAAGT
    ATTGAGCAAC ATAAACTGGG GACCAATAAA ACCCTGCTGA AGCAGGTTCG AGTAGTTGGA
    GTTACCTGTG CAGCCTGCCC ATTTCCATGC ATGAATGACC TTAAATTTCC TGTGGTTGTA
    CTGGATGAGT GTAGCCAAAT AACCGAACCA GCTTCTCTCC TTCCCATTGC AAGGTTTGAG
    TGTGAAAAGC TTATTCTTGT TGGAGATCCC AAACAGCTTC CTCCTACTAT TCAGGGTTCT
    GATGCAGCTC ATGAAAATGG ACTGGAACAA ACTCTTTTTG ATCGACTTTG CTTAATGGGT
    CACAAGCCAG TTCTTTTGCG AACCCAGTAC CGTTGTCACC CTACAATCAG TGCTATTGCT
    AATGATCTGT TTTATGAAGG AAACCTCGTG AATGGAGTTT CAGAAACAGA GAGGAGCCCT
    TTATTGGAAT GGCTTCCAAC CCTGTGTTTC TATAACATTC AAGGGCTGGA ACAGATCGAA
    AGAGATAACA GCTTTCATAA CGTAGCAGAA GCTGCTTTTA CACTCAAGCT GATTCAATCA
    CTGATTGTGA GTGGAATAGC AGGCTCTATG ATTGGGGTGA TAACATTATA CAAATCCCAG
    ATGTACAAGC TTTGTCACTT ACTCACTGCA CTGGACTTTG ACCATCCTGG TAATGTGAAA
    GCTGTGCAGG TGTCCACAGT AGATGCTTTT CAGGGCGCTG AAAAGGAGAT CATCATTCTG
    TCCTGTGTGA GGACAAGACA AGTAGGATTT ATTGATTCAG AACAAAGAAT GAATGTTGCA
    TTGACCAGAG GAAGGAGACA TTTGTTGATT GTGGGAAATT TGGCCTGTTT GAGGAAAAAT
    CGACTATGGG GGCGTGTGAT CCAACACTGT GAAGGCAGGG AAGATGGATT GCAACATGCA
    AGCCAATATG AACCACAGCT CAACCATCTC CTTAAAGATT ATTTTGAAAA ACAAGCAGAA
    GAAAAACAAA AGAAAAAGAA TGAAAAAGAT AAATCCAAAG ATAAATCTCA TTTACAAAAA
    GAAACAGTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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