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TNF cDNA ORF clone, Sus scrofa(Pig)

Gene Symbol TNF
Entrez Gene ID 397086
Full Name tumor necrosis factor
Synonyms TNFa
Gene Type protein-coding
Organism Sus scrofa(Pig)
Genome

7

7|7p11-q11


mRNA and Protein(s)

mRNA Protein Name
NM_214022.1 NP_999187.1 tumor necrosis factor



Related articles in PubMed

Effects of the -791(C→T) mutation in the promoter for tumor necrosis factor alpha on gene expression and resistance of Large White pigs to enterotoxigenic Escherichia coli F18.
Liu Y, Dai C, Sun L, Zhu G, Wu S, Bao W
Canadian journal of veterinary research = Revue canadienne de recherche veterinaire80(3)203-8(2016 Jul)

Effects of interleukin 6 and tumor necrosis factor-α on the proliferation of porcine theca interna cells: Possible role of these cytokines in the pathogenesis of polycystic ovary syndrome.
Hong L, Zhang Y, Wang Q, Han Y, Teng X
Taiwanese journal of obstetrics & gynecology55(2)183-7(2016 Apr)

Both Nsp1β and Nsp11 are responsible for differential TNF-α production induced by porcine reproductive and respiratory syndrome virus strains with different pathogenicity in vitro.
He Q, Li Y, Zhou L, Ge X, Guo X, Yang H
Virus research20132-40(2015 Apr)

The effect of interleukin-1β, interleukin-6, and tumor necrosis factor-α on estradiol-17β release in the myometrium: the in vitro study on the pig model.
Franczak A, Wojciechowicz B, Kolakowska J, Kotwica G
Theriogenology81(2)266-74(2014 Jan)

MiRNA-181a regulates adipogenesis by targeting tumor necrosis factor-α (TNF-α) in the porcine model.
Li H, Chen X, Guan L, Qi Q, Shu G, Jiang Q, Yuan L, Xi Q, Zhang Y
PloS one8(10)e71568(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TNF-alpha induced MMP-13 expression by condylar cells might be involved in the degradation of the juvenile condyle.
Title: Cyclic Tensile Strain Reduces TNF-α Induced Expression of MMP-13 by Condylar Temporomandibular Joint Cells.

TNF-alpha, secreted from activated Monocytes, mediates the downregulation of OTX2 and essential retinal pigment epithelium genes.
Title: Activated monocytes resist elimination by retinal pigment epithelium and downregulate their OTX2 expression via TNF-α.

TMZ pretreatment effectively reduced the myocardial damage caused by CME via inhibiting the PDCD4/NF-kappaB/ TNF-alpha pathway in cardiomyocytes.
Title: Effects of Trimetazidine on PDCD4/NF-κB/TNF-α Pathway in Coronary Microembolization.

This study showed that the -791(C-->T) mutation of the TNF-alpha gene could be considered an important potential genetic marker of enterotoxigenic Escherichia coli F18 resistance.
Title: Effects of the -791(C→T) mutation in the promoter for tumor necrosis factor alpha on gene expression and resistance of Large White pigs to enterotoxigenic Escherichia coli F18.

TNF-alpha was able to promote theca interna cell proliferation. Our results suggest that TNF-alpha might play a role in hyperandrogenism, cortex thickness, and the increased ovary volume observed in polycystic ovaries.
Title: Effects of interleukin 6 and tumor necrosis factor-α on the proliferation of porcine theca interna cells: Possible role of these cytokines in the pathogenesis of polycystic ovary syndrome.

The following TNF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TNF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OSe30520 NM_214022.1
Latest version!
Sus scrofa tumor necrosis factor (TNF), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $174.30
$249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OSe30520
Clone ID Related Accession (Same CDS sequence) NM_214022.1
Accession Version NM_214022.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 699bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-06
Organism Sus scrofa(Pig)
Product tumor necrosis factor
Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X54859.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X57321.1, EU682384.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02904337 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
ATGAGCACTG AGAGCATGAT CCGAGACGTG GAGCTGGCGG AGGAGGCGCT CGCCAAGAAG 
GCCGGGGGCC CCCAGGGCTC CAGGAGGTGC CTGTGCCTCA GCCTCTTCTC CTTCCTCCTG
GTCGCAGGAG CCACCACGCT CTTCTGCCTA CTGCACTTCG AGGTTATCGG CCCCCAGAAG
GAAGAGTTTC CAGCTGGCCC CTTGAGCATC AACCCTCTGG CCCAAGGACT CAGATCATCG
TCTCAAACCT CAGATAAGCC CGTCGCCCAC GTTGTAGCCA ATGTCAAAGC CGAGGGACAG
CTCCAATGGC AGAGTGGGTA TGCCAATGCC CTCCTGGCCA ACGGCGTGAA GCTGAAAGAC
AACCAGCTGG TGGTGCCGAC AGATGGGCTG TACCTCATCT ACTCCCAGGT CCTCTTCAGG
GGCCAAGGCT GCCCTTCCAC CAACGTTTTC CTCACTCACA CCATCAGCCG CATCGCCGTC
TCCTACCAGA CCAAGGTCAA CCTCCTCTCT GCCATCAAGA GCCCTTGCCA GAGGGAGACC
CCCGAGGGGG CCGAGGCCAA GCCCTGGTAC GAACCCATCT ACCTGGGAGG GGTCTTCCAG
CTGGAGAAGG ATGATCGACT CAGTGCCGAG ATCAACCTGC CCGACTATCT GGACTTTGCT
GAATCTGGGC AGGTCTATTT TGGGATCATT GCCCTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_999187.1
CDS184..882
Misc Feature(1)67..69
Misc Feature(2)187..189
Misc Feature(3)283..288
Misc Feature(4)289..351
Misc Feature(5)298..303
Misc Feature(6)328..333
Misc Feature(7)334..339
Misc Feature(8)409..414
Misc Feature(9)order(451..453,577..579,583..585,763..765,778..780,859..861,871..873)
Misc Feature(10)484..879
Misc Feature(11)order(493..498,511..513,637..639,658..660,673..675)
Translation

Target ORF information:

RefSeq Version NM_214022.1
Organism Sus scrofa(Pig)
Definition Sus scrofa tumor necrosis factor (TNF), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_214022.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGAGCACTG AGAGCATGAT CCGAGACGTG GAGCTGGCGG AGGAGGCGCT CGCCAAGAAG 
GCCGGGGGCC CCCAGGGCTC CAGGAGGTGC CTGTGCCTCA GCCTCTTCTC CTTCCTCCTG
GTCGCAGGAG CCACCACGCT CTTCTGCCTA CTGCACTTCG AGGTTATCGG CCCCCAGAAG
GAAGAGTTTC CAGCTGGCCC CTTGAGCATC AACCCTCTGG CCCAAGGACT CAGATCATCG
TCTCAAACCT CAGATAAGCC CGTCGCCCAC GTTGTAGCCA ATGTCAAAGC CGAGGGACAG
CTCCAATGGC AGAGTGGGTA TGCCAATGCC CTCCTGGCCA ACGGCGTGAA GCTGAAAGAC
AACCAGCTGG TGGTGCCGAC AGATGGGCTG TACCTCATCT ACTCCCAGGT CCTCTTCAGG
GGCCAAGGCT GCCCTTCCAC CAACGTTTTC CTCACTCACA CCATCAGCCG CATCGCCGTC
TCCTACCAGA CCAAGGTCAA CCTCCTCTCT GCCATCAAGA GCCCTTGCCA GAGGGAGACC
CCCGAGGGGG CCGAGGCCAA GCCCTGGTAC GAACCCATCT ACCTGGGAGG GGTCTTCCAG
CTGGAGAAGG ATGATCGACT CAGTGCCGAG ATCAACCTGC CCGACTATCT GGACTTTGCT
GAATCTGGGC AGGTCTATTT TGGGATCATT GCCCTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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