me3 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

  • Gene

  • Clones

  • gRNAs

The following me3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the me3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa25658 NM_001113837.1
Latest version!
Xenopus tropicalis malic enzyme 3, NADP(+)-dependent, mitochondrial (me3), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OXa51112 XM_031895653.1
Latest version!
Xenopus tropicalis malic enzyme 3, NADP(+)-dependent, mitochondrial (me3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OXa51113 XM_031895654.1
Latest version!
Xenopus tropicalis malic enzyme 3, NADP(+)-dependent, mitochondrial (me3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa25658
    Clone ID Related Accession (Same CDS sequence) NM_001113837.1
    Accession Version NM_001113837.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1842bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Xenopus tropicalis(tropical clawed frog)
    Product NADP-dependent malic enzyme, mitochondrial
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC155705.1. ##Evidence-Data-START## Transcript exon combination :: BC155705.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028291 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAACAGTG TGATCAGCAA GCAAGTCATG TCTGTGGGAC TCAGAGCCGC CAGTGGGGCA 
    GCCAGGTTGG GATTATGGGC ACCGGTCCTT AGCCACGCCC AGCCCTCCAT TAGGGTGTGC
    CACACAGCTG CCGGTGGGGG GACCCCGGTT AAGCCTCAGG CCATAAAGAA GAGGGGCTAT
    GACATCACCA GAAACCCCTA TCTCAACAAG GGCATGGCAT TTACGCTTGA AGAGAGGCTC
    CAGCTGGGCA TTCACGGCCT CCTCCCTCCC TGCTTCCTCA GCCAAGATGT ACAAGTTCTA
    CGAGTTATGA AAAGTTATGA AACCAAGTCC AGCGATCTAG ATAAATATAT CATCCTGATG
    ACCTTACAAG ACAGGAACGA GAAGCTCTTC TATCGCGTCC TTACCTCTGA CATTGAGAGG
    TTCATGCCCA TTGTGTACAC GCCAACTGTT GGGCTGGCTT GTCAGCAGTA TGGACTTGCT
    TTCAGGAGAC CACGAGGTTT ATTTATTACT ATTCACGACA AAGGTCACAT TGCAACCATG
    CTGAACTCCT GGCCGGAGGA AGATATTAAG GCAATAGTAG TGACAGACGG TGAGCGTATC
    CTCGGCCTTG GCGATCTCGG CGGCTACGGC ATGGGAATCC CCGTGGGAAA GCTTGCCCTA
    TACACAGCCT GTGGCGGCGT CCACCCACAG CAGTGCCTTC CCGTACTCCT CGATGTAGGC
    ACCGACAATG AGGCTCTTTT GAATGATCCC CTATATATCG GGCTAAAGCA CAAGCGGGTT
    CGTGGGAAGC AATACGATGA GCTGATCGAC GAGTTTATGC AAGCGGTGAC TAACAAGTAC
    GGGATGAACT GCCTGATCCA GTTTGAAGAC TTTGCCAACT CCAATGCATT CCGTCTGCTG
    AACAAGTATC GGAACAAGTA CTGCACCTTT AATGATGATA TTCAAGGAAC GGCCTCCGTG
    GCAGTAGCGG GAATCCTGGC AGCGCTTCGC ATCACAAACA ATAGACTTTC TGATCATAAG
    TTTGTTTTCC AAGGAGCTGG GGAGGCTGCC ATGGGAATCG CACAGCTCAT CACAATGGCC
    ATGGAAAAGG AAGGAACCTC CAGGGAAGAC GCAATCAAGA AGATCTGGAT GGTTGATTCC
    AAGGGACTGA TAGTAAAGGG AAGGGGCAAT CTGAACCATG AGAAAGACGT GTTTGCCCAT
    GATCACCCTC AAGTAAAAAC CTTGGAAGAA GCTGTGCAGA TCCTGAAGCC AACAGCCATC
    ATAGGTGTAG CTGCAATCGG CGGAGCATTT ACAGAAAAGA TAATAAAAGA CATGGCCGCT
    TTCAACAAAC GACCAATCAT ATTCGCTCTT AGCAACCCAA CGAGTAAGGC GGAATGTACA
    GCAGAGCAGT GCTACCAGCT GACTGAGGGT CGGGGAATAT TTGCGAGCGG AAGCCCTTTC
    TCTAAGGTGA CTCTTGCAAA CGGCCAAACA TTTTACCCCG GGCAGGGTAA CAACGCCTAC
    GTATTCCCCG GTGTGGCGCT GGGGGTCATT GGATGTGGAG TCCGGCACAT CTCGGAGGAT
    TTATTTCTTA CGACAGCTGA GACCATTGCA GAGATGGTTA CAGCTGAGAA TTTAGCAGAA
    GGAAGGCTTT ATCCTCCCCT GAGCTCCATC AGAGATGTGT CCTTCAAAAT TGCTGTCAAA
    ATTGTAGATT ACGCCTACAA AAACAACATG GCTTCCTGGT ACCCCGAGCC GGAGGATAAG
    GAGTCTTTCG TCCGATCCCT CATTTACAGC CCTGATTATG ATTCGTTTAC CATCGACAGC
    TACAATTGGC CCGCTCAGGC TATGGAAATC CAAGATGTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001107309.1
    CDS193..2034
    Translation

    Target ORF information:

    RefSeq Version NM_001113837.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis malic enzyme 3, NADP(+)-dependent, mitochondrial (me3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001113837.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAACAGTG TGATCAGCAA GCAAGTCATG TCTGTGGGAC TCAGAGCCGC CAGTGGGGCA 
    GCCAGGTTGG GATTATGGGC ACCGGTCCTT AGCCACGCCC AGCCCTCCAT TAGGGTGTGC
    CACACAGCTG CCGGTGGGGG GACCCCGGTT AAGCCTCAGG CCATAAAGAA GAGGGGCTAT
    GACATCACCA GAAACCCCTA TCTCAACAAG GGCATGGCAT TTACGCTTGA AGAGAGGCTC
    CAGCTGGGCA TTCACGGCCT CCTCCCTCCC TGCTTCCTCA GCCAAGATGT ACAAGTTCTA
    CGAGTTATGA AAAGTTATGA AACCAAGTCC AGCGATCTAG ATAAATATAT CATCCTGATG
    ACCTTACAAG ACAGGAACGA GAAGCTCTTC TATCGCGTCC TTACCTCTGA CATTGAGAGG
    TTCATGCCCA TTGTGTACAC GCCAACTGTT GGGCTGGCTT GTCAGCAGTA TGGACTTGCT
    TTCAGGAGAC CACGAGGTTT ATTTATTACT ATTCACGACA AAGGTCACAT TGCAACCATG
    CTGAACTCCT GGCCGGAGGA AGATATTAAG GCAATAGTAG TGACAGACGG TGAGCGTATC
    CTCGGCCTTG GCGATCTCGG CGGCTACGGC ATGGGAATCC CCGTGGGAAA GCTTGCCCTA
    TACACAGCCT GTGGCGGCGT CCACCCACAG CAGTGCCTTC CCGTACTCCT CGATGTAGGC
    ACCGACAATG AGGCTCTTTT GAATGATCCC CTATATATCG GGCTAAAGCA CAAGCGGGTT
    CGTGGGAAGC AATACGATGA GCTGATCGAC GAGTTTATGC AAGCGGTGAC TAACAAGTAC
    GGGATGAACT GCCTGATCCA GTTTGAAGAC TTTGCCAACT CCAATGCATT CCGTCTGCTG
    AACAAGTATC GGAACAAGTA CTGCACCTTT AATGATGATA TTCAAGGAAC GGCCTCCGTG
    GCAGTAGCGG GAATCCTGGC AGCGCTTCGC ATCACAAACA ATAGACTTTC TGATCATAAG
    TTTGTTTTCC AAGGAGCTGG GGAGGCTGCC ATGGGAATCG CACAGCTCAT CACAATGGCC
    ATGGAAAAGG AAGGAACCTC CAGGGAAGAC GCAATCAAGA AGATCTGGAT GGTTGATTCC
    AAGGGACTGA TAGTAAAGGG AAGGGGCAAT CTGAACCATG AGAAAGACGT GTTTGCCCAT
    GATCACCCTC AAGTAAAAAC CTTGGAAGAA GCTGTGCAGA TCCTGAAGCC AACAGCCATC
    ATAGGTGTAG CTGCAATCGG CGGAGCATTT ACAGAAAAGA TAATAAAAGA CATGGCCGCT
    TTCAACAAAC GACCAATCAT ATTCGCTCTT AGCAACCCAA CGAGTAAGGC GGAATGTACA
    GCAGAGCAGT GCTACCAGCT GACTGAGGGT CGGGGAATAT TTGCGAGCGG AAGCCCTTTC
    TCTAAGGTGA CTCTTGCAAA CGGCCAAACA TTTTACCCCG GGCAGGGTAA CAACGCCTAC
    GTATTCCCCG GTGTGGCGCT GGGGGTCATT GGATGTGGAG TCCGGCACAT CTCGGAGGAT
    TTATTTCTTA CGACAGCTGA GACCATTGCA GAGATGGTTA CAGCTGAGAA TTTAGCAGAA
    GGAAGGCTTT ATCCTCCCCT GAGCTCCATC AGAGATGTGT CCTTCAAAAT TGCTGTCAAA
    ATTGTAGATT ACGCCTACAA AAACAACATG GCTTCCTGGT ACCCCGAGCC GGAGGATAAG
    GAGTCTTTCG TCCGATCCCT CATTTACAGC CCTGATTATG ATTCGTTTAC CATCGACAGC
    TACAATTGGC CCGCTCAGGC TATGGAAATC CAAGATGTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa51112
    Clone ID Related Accession (Same CDS sequence) XM_031895653.1
    Accession Version XM_031895653.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1875bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product NADP-dependent malic enzyme, mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGACATTTA GGGCGACACA TCTGAGATAC AGAATGAACA GTGTGATCAG CAAGCAAGTC 
    ATGTCTGTGG GACTCAGAGC CGCCAGTGGG GCAGCCAGGT TGGGATTATG GGCACCGGTC
    CTTAGCCACG CCCAGCCCTC CATTAGGGTG TGCCACACAG CTGCCGGTGG GGGGACCCCG
    GTTAAGCCTC AGGCCATAAA GAAGAGGGGC TATGACATCA CCAGAAACCC CTATCTCAAC
    AAGGGCATGG CATTTACGCT TGAAGAGAGG CTCCAGCTGG GCATTCACGG CCTCCTCCCT
    CCCTGCTTCC TCAGCCAAGA TGTACAAGTT CTACGAGTTA TGAAAAGTTA TGAAACCAAG
    TCCAGCGATC TAGATAAATA TATCATCCTG ATGACCTTAC AAGACAGGAA CGAGAAGCTC
    TTCTATCGCG TCCTTACCTC TGACATTGAG AGGTTCATGC CCATTGTGTA CACGCCAACT
    GTTGGGCTGG CTTGTCAGCA GTATGGACTT GCTTTCAGGA GACCACGAGG TTTATTTATT
    ACTATTCACG ACAAAGGTCA CATTGCAACC ATGCTGAACT CCTGGCCGGA GGAAGATATT
    AAGGCAATAG TAGTGACAGA CGGTGAGCGT ATCCTTGGCC TTGGCGATCT CGGCGGCTAC
    GGCATGGGAA TCCCCGTGGG AAAGCTTGCC CTATACACAG CCTGTGGCGG CGTCCACCCA
    CAGCAGTGCC TTCCCGTACT CCTCGATGTA GGCACCGACA ATGAGGCTCT TTTGAATGAT
    CCCCTATATA TCGGGCTAAA GCACAAGCGG GTTCGTGGGA AGCAGTACGA TGAGCTGATC
    GACGAGTTTA TGCAAGCGGT GACTAACAAG TACGGGATGA ACTGCCTGAT CCAGTTTGAA
    GACTTTGCCA ACTCCAATGC ATTCCGTCTG CTGAACAAGT ATCGGAACAA GTACTGCACC
    TTTAATGATG ATATTCAAGG AACGGCCTCC GTGGCAGTGG CGGGAATCCT GGCAGCGCTT
    CGCATCACAA ACAATAGACT TTCTGATCAT AAGTTTGTTT TCCAAGGAGC TGGGGAGGCT
    GCCATGGGAA TCGCACAGCT CATCACAATG GCCATGGAAA AGGAAGGAAC CTCCAGGGAA
    GACGCAATCA AGAAGATCTG GATGGTTGAT TCCAAGGGAC TGATAGTAAA GGGAAGGGGC
    AATCTGAACC ATGAGAAAGA CGTGTTTGCC CATGATCACC CTCAAGTAAA AACCTTGGAA
    GAAGCTGTGC AGATCCTGAA GCCAACAGCC ATCATAGGTG TAGCTGCAAT CGGCGGAGCA
    TTTACAGAAA AGATAATAAA AGACATGGCC GCTTTCAACA AACGACCAAT CATATTCGCT
    CTTAGCAACC CAACGAGTAA GGCGGAATGT ACAGCAGAGC AGTGCTACCA GCTGACTGAG
    GGTCGGGGAA TATTTGCGAG CGGAAGCCCT TTCTCTAAGG TGACTCTTGC AAACGGCCAA
    ACATTTTACC CCGGGCAGGG TAACAACGCC TACGTATTCC CCGGTGTGGC GCTGGGGGTC
    ATTGGATGTG GAGTCCGGCA CATCTCGGAG GATTTATTTC TTACGACAGC TGAGACCATT
    GCAGAGATGG TTACAGCTGA GAATTTAGCA GAAGGAAGGC TTTATCCTCC CCTGAGCTCC
    ATCAGAGATG TGTCCTTCAA AATTGCTGTC AAAATTGTAG ATTACGCCTA CAAAAACAAC
    ATGGCTTCCT GGTACCCCGA GCCGGAGGAT AAGGAGTCTT TCGTCCGATC CCTCATTTAC
    AGCCCTGATT ATGATTCGTT TACCATCGAC AGCTACAATT GGCCCGCTCA GGCTATGGAA
    ATCCAAGATG TATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751513.1
    CDS69..1943
    Translation

    Target ORF information:

    RefSeq Version XM_031895653.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis malic enzyme 3, NADP(+)-dependent, mitochondrial (me3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031895653.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGACATTTA GGGCGACACA TCTGAGATAC AGAATGAACA GTGTGATCAG CAAGCAAGTC 
    ATGTCTGTGG GACTCAGAGC CGCCAGTGGG GCAGCCAGGT TGGGATTATG GGCACCGGTC
    CTTAGCCACG CCCAGCCCTC CATTAGGGTG TGCCACACAG CTGCCGGTGG GGGGACCCCG
    GTTAAGCCTC AGGCCATAAA GAAGAGGGGC TATGACATCA CCAGAAACCC CTATCTCAAC
    AAGGGCATGG CATTTACGCT TGAAGAGAGG CTCCAGCTGG GCATTCACGG CCTCCTCCCT
    CCCTGCTTCC TCAGCCAAGA TGTACAAGTT CTACGAGTTA TGAAAAGTTA TGAAACCAAG
    TCCAGCGATC TAGATAAATA TATCATCCTG ATGACCTTAC AAGACAGGAA CGAGAAGCTC
    TTCTATCGCG TCCTTACCTC TGACATTGAG AGGTTCATGC CCATTGTGTA CACGCCAACT
    GTTGGGCTGG CTTGTCAGCA GTATGGACTT GCTTTCAGGA GACCACGAGG TTTATTTATT
    ACTATTCACG ACAAAGGTCA CATTGCAACC ATGCTGAACT CCTGGCCGGA GGAAGATATT
    AAGGCAATAG TAGTGACAGA CGGTGAGCGT ATCCTTGGCC TTGGCGATCT CGGCGGCTAC
    GGCATGGGAA TCCCCGTGGG AAAGCTTGCC CTATACACAG CCTGTGGCGG CGTCCACCCA
    CAGCAGTGCC TTCCCGTACT CCTCGATGTA GGCACCGACA ATGAGGCTCT TTTGAATGAT
    CCCCTATATA TCGGGCTAAA GCACAAGCGG GTTCGTGGGA AGCAGTACGA TGAGCTGATC
    GACGAGTTTA TGCAAGCGGT GACTAACAAG TACGGGATGA ACTGCCTGAT CCAGTTTGAA
    GACTTTGCCA ACTCCAATGC ATTCCGTCTG CTGAACAAGT ATCGGAACAA GTACTGCACC
    TTTAATGATG ATATTCAAGG AACGGCCTCC GTGGCAGTGG CGGGAATCCT GGCAGCGCTT
    CGCATCACAA ACAATAGACT TTCTGATCAT AAGTTTGTTT TCCAAGGAGC TGGGGAGGCT
    GCCATGGGAA TCGCACAGCT CATCACAATG GCCATGGAAA AGGAAGGAAC CTCCAGGGAA
    GACGCAATCA AGAAGATCTG GATGGTTGAT TCCAAGGGAC TGATAGTAAA GGGAAGGGGC
    AATCTGAACC ATGAGAAAGA CGTGTTTGCC CATGATCACC CTCAAGTAAA AACCTTGGAA
    GAAGCTGTGC AGATCCTGAA GCCAACAGCC ATCATAGGTG TAGCTGCAAT CGGCGGAGCA
    TTTACAGAAA AGATAATAAA AGACATGGCC GCTTTCAACA AACGACCAAT CATATTCGCT
    CTTAGCAACC CAACGAGTAA GGCGGAATGT ACAGCAGAGC AGTGCTACCA GCTGACTGAG
    GGTCGGGGAA TATTTGCGAG CGGAAGCCCT TTCTCTAAGG TGACTCTTGC AAACGGCCAA
    ACATTTTACC CCGGGCAGGG TAACAACGCC TACGTATTCC CCGGTGTGGC GCTGGGGGTC
    ATTGGATGTG GAGTCCGGCA CATCTCGGAG GATTTATTTC TTACGACAGC TGAGACCATT
    GCAGAGATGG TTACAGCTGA GAATTTAGCA GAAGGAAGGC TTTATCCTCC CCTGAGCTCC
    ATCAGAGATG TGTCCTTCAA AATTGCTGTC AAAATTGTAG ATTACGCCTA CAAAAACAAC
    ATGGCTTCCT GGTACCCCGA GCCGGAGGAT AAGGAGTCTT TCGTCCGATC CCTCATTTAC
    AGCCCTGATT ATGATTCGTT TACCATCGAC AGCTACAATT GGCCCGCTCA GGCTATGGAA
    ATCCAAGATG TATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa51113
    Clone ID Related Accession (Same CDS sequence) XM_031895654.1
    Accession Version XM_031895654.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1842bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product NADP-dependent malic enzyme, mitochondrial isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAACAGTG TGATCAGCAA GCAAGTCATG TCTGTGGGAC TCAGAGCCGC CAGTGGGGCA 
    GCCAGGTTGG GATTATGGGC ACCGGTCCTT AGCCACGCCC AGCCCTCCAT TAGGGTGTGC
    CACACAGCTG CCGGTGGGGG GACCCCGGTT AAGCCTCAGG CCATAAAGAA GAGGGGCTAT
    GACATCACCA GAAACCCCTA TCTCAACAAG GGCATGGCAT TTACGCTTGA AGAGAGGCTC
    CAGCTGGGCA TTCACGGCCT CCTCCCTCCC TGCTTCCTCA GCCAAGATGT ACAAGTTCTA
    CGAGTTATGA AAAGTTATGA AACCAAGTCC AGCGATCTAG ATAAATATAT CATCCTGATG
    ACCTTACAAG ACAGGAACGA GAAGCTCTTC TATCGCGTCC TTACCTCTGA CATTGAGAGG
    TTCATGCCCA TTGTGTACAC GCCAACTGTT GGGCTGGCTT GTCAGCAGTA TGGACTTGCT
    TTCAGGAGAC CACGAGGTTT ATTTATTACT ATTCACGACA AAGGTCACAT TGCAACCATG
    CTGAACTCCT GGCCGGAGGA AGATATTAAG GCAATAGTAG TGACAGACGG TGAGCGTATC
    CTTGGCCTTG GCGATCTCGG CGGCTACGGC ATGGGAATCC CCGTGGGAAA GCTTGCCCTA
    TACACAGCCT GTGGCGGCGT CCACCCACAG CAGTGCCTTC CCGTACTCCT CGATGTAGGC
    ACCGACAATG AGGCTCTTTT GAATGATCCC CTATATATCG GGCTAAAGCA CAAGCGGGTT
    CGTGGGAAGC AGTACGATGA GCTGATCGAC GAGTTTATGC AAGCGGTGAC TAACAAGTAC
    GGGATGAACT GCCTGATCCA GTTTGAAGAC TTTGCCAACT CCAATGCATT CCGTCTGCTG
    AACAAGTATC GGAACAAGTA CTGCACCTTT AATGATGATA TTCAAGGAAC GGCCTCCGTG
    GCAGTGGCGG GAATCCTGGC AGCGCTTCGC ATCACAAACA ATAGACTTTC TGATCATAAG
    TTTGTTTTCC AAGGAGCTGG GGAGGCTGCC ATGGGAATCG CACAGCTCAT CACAATGGCC
    ATGGAAAAGG AAGGAACCTC CAGGGAAGAC GCAATCAAGA AGATCTGGAT GGTTGATTCC
    AAGGGACTGA TAGTAAAGGG AAGGGGCAAT CTGAACCATG AGAAAGACGT GTTTGCCCAT
    GATCACCCTC AAGTAAAAAC CTTGGAAGAA GCTGTGCAGA TCCTGAAGCC AACAGCCATC
    ATAGGTGTAG CTGCAATCGG CGGAGCATTT ACAGAAAAGA TAATAAAAGA CATGGCCGCT
    TTCAACAAAC GACCAATCAT ATTCGCTCTT AGCAACCCAA CGAGTAAGGC GGAATGTACA
    GCAGAGCAGT GCTACCAGCT GACTGAGGGT CGGGGAATAT TTGCGAGCGG AAGCCCTTTC
    TCTAAGGTGA CTCTTGCAAA CGGCCAAACA TTTTACCCCG GGCAGGGTAA CAACGCCTAC
    GTATTCCCCG GTGTGGCGCT GGGGGTCATT GGATGTGGAG TCCGGCACAT CTCGGAGGAT
    TTATTTCTTA CGACAGCTGA GACCATTGCA GAGATGGTTA CAGCTGAGAA TTTAGCAGAA
    GGAAGGCTTT ATCCTCCCCT GAGCTCCATC AGAGATGTGT CCTTCAAAAT TGCTGTCAAA
    ATTGTAGATT ACGCCTACAA AAACAACATG GCTTCCTGGT ACCCCGAGCC GGAGGATAAG
    GAGTCTTTCG TCCGATCCCT CATTTACAGC CCTGATTATG ATTCGTTTAC CATCGACAGC
    TACAATTGGC CCGCTCAGGC TATGGAAATC CAAGATGTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751514.1
    CDS192..2033
    Translation

    Target ORF information:

    RefSeq Version XM_031895654.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis malic enzyme 3, NADP(+)-dependent, mitochondrial (me3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031895654.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAACAGTG TGATCAGCAA GCAAGTCATG TCTGTGGGAC TCAGAGCCGC CAGTGGGGCA 
    GCCAGGTTGG GATTATGGGC ACCGGTCCTT AGCCACGCCC AGCCCTCCAT TAGGGTGTGC
    CACACAGCTG CCGGTGGGGG GACCCCGGTT AAGCCTCAGG CCATAAAGAA GAGGGGCTAT
    GACATCACCA GAAACCCCTA TCTCAACAAG GGCATGGCAT TTACGCTTGA AGAGAGGCTC
    CAGCTGGGCA TTCACGGCCT CCTCCCTCCC TGCTTCCTCA GCCAAGATGT ACAAGTTCTA
    CGAGTTATGA AAAGTTATGA AACCAAGTCC AGCGATCTAG ATAAATATAT CATCCTGATG
    ACCTTACAAG ACAGGAACGA GAAGCTCTTC TATCGCGTCC TTACCTCTGA CATTGAGAGG
    TTCATGCCCA TTGTGTACAC GCCAACTGTT GGGCTGGCTT GTCAGCAGTA TGGACTTGCT
    TTCAGGAGAC CACGAGGTTT ATTTATTACT ATTCACGACA AAGGTCACAT TGCAACCATG
    CTGAACTCCT GGCCGGAGGA AGATATTAAG GCAATAGTAG TGACAGACGG TGAGCGTATC
    CTTGGCCTTG GCGATCTCGG CGGCTACGGC ATGGGAATCC CCGTGGGAAA GCTTGCCCTA
    TACACAGCCT GTGGCGGCGT CCACCCACAG CAGTGCCTTC CCGTACTCCT CGATGTAGGC
    ACCGACAATG AGGCTCTTTT GAATGATCCC CTATATATCG GGCTAAAGCA CAAGCGGGTT
    CGTGGGAAGC AGTACGATGA GCTGATCGAC GAGTTTATGC AAGCGGTGAC TAACAAGTAC
    GGGATGAACT GCCTGATCCA GTTTGAAGAC TTTGCCAACT CCAATGCATT CCGTCTGCTG
    AACAAGTATC GGAACAAGTA CTGCACCTTT AATGATGATA TTCAAGGAAC GGCCTCCGTG
    GCAGTGGCGG GAATCCTGGC AGCGCTTCGC ATCACAAACA ATAGACTTTC TGATCATAAG
    TTTGTTTTCC AAGGAGCTGG GGAGGCTGCC ATGGGAATCG CACAGCTCAT CACAATGGCC
    ATGGAAAAGG AAGGAACCTC CAGGGAAGAC GCAATCAAGA AGATCTGGAT GGTTGATTCC
    AAGGGACTGA TAGTAAAGGG AAGGGGCAAT CTGAACCATG AGAAAGACGT GTTTGCCCAT
    GATCACCCTC AAGTAAAAAC CTTGGAAGAA GCTGTGCAGA TCCTGAAGCC AACAGCCATC
    ATAGGTGTAG CTGCAATCGG CGGAGCATTT ACAGAAAAGA TAATAAAAGA CATGGCCGCT
    TTCAACAAAC GACCAATCAT ATTCGCTCTT AGCAACCCAA CGAGTAAGGC GGAATGTACA
    GCAGAGCAGT GCTACCAGCT GACTGAGGGT CGGGGAATAT TTGCGAGCGG AAGCCCTTTC
    TCTAAGGTGA CTCTTGCAAA CGGCCAAACA TTTTACCCCG GGCAGGGTAA CAACGCCTAC
    GTATTCCCCG GTGTGGCGCT GGGGGTCATT GGATGTGGAG TCCGGCACAT CTCGGAGGAT
    TTATTTCTTA CGACAGCTGA GACCATTGCA GAGATGGTTA CAGCTGAGAA TTTAGCAGAA
    GGAAGGCTTT ATCCTCCCCT GAGCTCCATC AGAGATGTGT CCTTCAAAAT TGCTGTCAAA
    ATTGTAGATT ACGCCTACAA AAACAACATG GCTTCCTGGT ACCCCGAGCC GGAGGATAAG
    GAGTCTTTCG TCCGATCCCT CATTTACAGC CCTGATTATG ATTCGTTTAC CATCGACAGC
    TACAATTGGC CCGCTCAGGC TATGGAAATC CAAGATGTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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