• English
  • Sign In
    Please Sign In
    Sign In
    New to GenScript?
    Register
  • Contact Us
×
Contact us now: +1-877-436-7274

eef2k cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Summary

Gene Symbol eef2k
Entrez Gene ID 100145184
Full Name eukaryotic elongation factor 2 kinase
General protein information
Preferred Names

eukaryotic elongation factor 2 kinase

Names

eukaryotic elongation factor 2 kinase
Gene Type protein-coding
Organism Xenopus tropicalis(tropical clawed frog)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_012970072.3 XP_012825526.1 eukaryotic elongation factor 2 kinase isoform X1
XM_012970073.2 XP_012825527.1 eukaryotic elongation factor 2 kinase isoform X2
XM_012970074.3 XP_012825528.1 eukaryotic elongation factor 2 kinase isoform X3
XM_012970074.2 XP_012825528.1 eukaryotic elongation factor 2 kinase isoform X3
XM_012970072.2 XP_012825526.1 eukaryotic elongation factor 2 kinase isoform X1
XM_012970073.3 XP_012825527.1 eukaryotic elongation factor 2 kinase isoform X2
NM_001126674.1 NP_001120146.1 eukaryotic elongation factor 2 kinase

Pathways from BioSystems


Homologies


Homo sapiens (human) EEF2K NP_037434.1
Bos taurus (cattle) EEF2K NP_001179471.1
EEF2K XP_001092565.1
Canis lupus familiaris (dog) EEF2K XP_005621491.1
Pan troglodytes (chimpanzee) EEF2K XP_001161085.2
Rattus norvegicus (Norway rat) Eef2k NP_037079.1
Danio rerio (zebrafish) eef2k NP_001002740.1
Caenorhabditis elegans (roundworm) efk-1 NP_001022572.1
Xenopus tropicalis (tropical clawed frog) eef2k NP_001120146.1
Mus musculus (house mouse) Eef2k NP_001254640.1
Gallus gallus (chicken) EEF2K XP_004945358.1

Gene Ontology


Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following eef2k gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the eef2k cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa01820 NM_001126674.1
Latest version!		 Xenopus tropicalis eukaryotic elongation factor 2 kinase (eef2k), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $426.30
$609.00
OXa31610 XM_012970072.2
Latest version!		 Xenopus tropicalis eukaryotic elongation factor 2 kinase (eef2k), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $426.30
$609.00
OXa31611 XM_012970073.2
Latest version!		 Xenopus tropicalis eukaryotic elongation factor 2 kinase (eef2k), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $426.30
$609.00
OXa31612 XM_012970074.2
Latest version!		 Xenopus tropicalis eukaryotic elongation factor 2 kinase (eef2k), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $426.30
$609.00
OXa65869 XM_012970072.3
Latest version!		 Xenopus tropicalis eukaryotic elongation factor 2 kinase (eef2k), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $391.30
$559.00
OXa65870 XM_012970073.3
Latest version!		 Xenopus tropicalis eukaryotic elongation factor 2 kinase (eef2k), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $391.30
$559.00
OXa65871 XM_012970074.3
Latest version!		 Xenopus tropicalis eukaryotic elongation factor 2 kinase (eef2k), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $391.30
$559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OXa01820
Clone ID Related Accession (Same CDS sequence) NM_001126674.1
Accession Version NM_001126674.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2157bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-21
Organism Xenopus tropicalis(tropical clawed frog)
Product eukaryotic elongation factor 2 kinase
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC159137.1. ##Evidence-Data-START## CDS exon combination :: BC159137.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMD00028292, SAMD00028293 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGGCAGACG AAGATCTCAT CTTTAACATG GAGGGCCTTG ATAACATCGA CTTGGCCAAA 
GACCGATGCT CAGATTACAA CAAGGCCGAC AGCGATGAGG AAGACAATTT CTACATCTGC 
CCTATCACCG ACGACCCAGC TGCGCAGCAC CAGACCAAAA AGAAAAGTCC ATGTTTCTTT 
ACCAACTTGG TGAAAAAGAC AGAATATGGA ACTAGTTCCT CCCCTCGGAA CAGTTTTAAG 
GAGGCCTGGA AGCATGCCAT TCAGAAAGCT AAGCAGATGC CTGATCCATG GGCTGAGTTT 
CACTTGGAAG ACATTGAGAC AGAGCATGCT ATCAGGCACA GGTACAATGC TGTATCTGGC 
CAGTGGGTTC AAGATGATGT ACTAATTAAA ATGGCCTCTC AGGCGTTTGG CAGGGGAGCC 
ATGAGAGAAT GTTACCGAAC GAAAAAACTC TCCAACTTCT CACACAAACA GCAGTGGAAG 
AGCGCATATA ATTATGTGTC CAAACGATAC TTAGAGACTG TGGACAGAGA TGTGTACTTT 
GAGGACGTCC GACTCCAGAT GGAGGCAAAG CTGTGGGGAG AGGAATACAA CAGACACAAA 
CCACCAAAAC AGGTGGATAT CATGCAAATG TGCATTATTG AAATGAAGGA CAGGCCTGGC 
AAACCGCTTT TTCACCTTGA ACATTACATC GAGGGAAAGT ATATTAAATA TAACTCAAAT 
TCTGGGTTTG TACGAGATGA CAATATCCGC CTCACTCCTC AGGCCTTCAG TCACTTCACA 
TTTGAGCGCT CAGGACATCA GCTGATTGTT GTTGATGTAC AAGGAGTTGG TGATTTATAC 
ACTGATCCTC AGATCCACAC AGAGAGTGGG ACGGATTTTG GAGATGGAAA TTTAGGGGTA 
CGAGGGATGG CTCTGTTTTT CCATTCTCAT GCTTGCAACA GAATTTGCAA AAGCATGGGA 
CTGACACCCT TTGACATTTC GGCCAAAGAA AAGGCTGTTT TGGATAACTG CAGCAAAATG 
TTGCAAACAG CCCAGACTGT TGTTCGTGGC ATTGAGGAAA AGTGTGGCAG CAATCGGGTC 
AGAACCATGT CTGGCAGCCG CCCACCTCTT CTCTCACGTC TCTCTGAAAA CTCTGGAGAT 
GACATGAGTG ACATGGCGTT TGATTCCTTA CCTAGCTCAC CTTCCCCAGT CACCCCCCAC 
GGCCATCGAT TCGATTCGCA TGGTTGGCCT ATTTTTAATG AGGTTGACAA CTTACTTAAC 
GAAGAAAACA ATAACTTGGA CAATCATAGA GACTCTGAAA ATGGAGGGGA TAGTGGATGT 
CCCAGTGAAA AGAGAAGTGA ATCTGATGAG CTGGAACACA GAATCCATAA CGTCGGCAGG 
AGACGTTTTG AGTCAGAATC CGAAGACAGT TTTGGAAAGG TCAGTGTGGA AAAATGGAAC 
CAGTTCCACG CTTCCCGAGT TCATGTCCAC AGACCCTCTT GTGTGGCGTT TGAAGTAGAA 
AGACTTAATG CTCTGGAACT AGAGAAGAAA ATCGGGAAAT CGATTCTAGG AAAGGTTCAC 
TTGGCCATGG TGCGTTATCA TGAAGGGGGG CGTTTCTGCG AGAAAGACCA AGAATGGGAT 
CAGGAATCTG CCATTTTTCA TCTGGAACAC GCTGCTCACT GTGGGGAACT TGAAGCCATT 
ATTGGCCTTG GCCTCATGTA CTCACAGTCA CCCCATCACA TACTGACTGA TGTATCTGTC 
CAGGACACAG AAGATAATAG GATAAAAGGA TTTAACTATT TGTTGATGGC AGCAGAAGCA 
GGAGACCGTC CTTCCATGAT TCACGTTGCC CGAGCTTTTG ATACTGGTGT GAACCTTGGT 
TCAGACAGGA TAAAGGACTG GCAAGAAGCC CTCTTCTGGT ACGATTCTGC ACTCAACATG 
ACAGATTATG ATGAAGTTGG TGAATATGAT GGAACCCAAG ATGAACCCAA ATACTTGCTG 
CTGGCTAGGG AAGCAGAAAT GTTACTTACT GGGGGATTTA ACCTTCAGAA GGACCCACAG 
CGATCTGGTG ACTTGTACAC AGAAGCTGCT GAAGCAGCAA TGGAAGCAAT GAAAGGGAGG 
TTGGCCAATC AGTACTACCA GAAGGCAGAG GAGGCCTGGG CGTGTATGGA GGAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001120146.1
CDS295..2451
Translation

Target ORF information:

RefSeq Version NM_001126674.1
Organism Xenopus tropicalis(tropical clawed frog)
Definition Xenopus tropicalis eukaryotic elongation factor 2 kinase (eef2k), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001126674.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGGCAGACG AAGATCTCAT CTTTAACATG GAGGGCCTTG ATAACATCGA CTTGGCCAAA 
GACCGATGCT CAGATTACAA CAAGGCCGAC AGCGATGAGG AAGACAATTT CTACATCTGC 
CCTATCACCG ACGACCCAGC TGCGCAGCAC CAGACCAAAA AGAAAAGTCC ATGTTTCTTT 
ACCAACTTGG TGAAAAAGAC AGAATATGGA ACTAGTTCCT CCCCTCGGAA CAGTTTTAAG 
GAGGCCTGGA AGCATGCCAT TCAGAAAGCT AAGCAGATGC CTGATCCATG GGCTGAGTTT 
CACTTGGAAG ACATTGAGAC AGAGCATGCT ATCAGGCACA GGTACAATGC TGTATCTGGC 
CAGTGGGTTC AAGATGATGT ACTAATTAAA ATGGCCTCTC AGGCGTTTGG CAGGGGAGCC 
ATGAGAGAAT GTTACCGAAC GAAAAAACTC TCCAACTTCT CACACAAACA GCAGTGGAAG 
AGCGCATATA ATTATGTGTC CAAACGATAC TTAGAGACTG TGGACAGAGA TGTGTACTTT 
GAGGACGTCC GACTCCAGAT GGAGGCAAAG CTGTGGGGAG AGGAATACAA CAGACACAAA 
CCACCAAAAC AGGTGGATAT CATGCAAATG TGCATTATTG AAATGAAGGA CAGGCCTGGC 
AAACCGCTTT TTCACCTTGA ACATTACATC GAGGGAAAGT ATATTAAATA TAACTCAAAT 
TCTGGGTTTG TACGAGATGA CAATATCCGC CTCACTCCTC AGGCCTTCAG TCACTTCACA 
TTTGAGCGCT CAGGACATCA GCTGATTGTT GTTGATGTAC AAGGAGTTGG TGATTTATAC 
ACTGATCCTC AGATCCACAC AGAGAGTGGG ACGGATTTTG GAGATGGAAA TTTAGGGGTA 
CGAGGGATGG CTCTGTTTTT CCATTCTCAT GCTTGCAACA GAATTTGCAA AAGCATGGGA 
CTGACACCCT TTGACATTTC GGCCAAAGAA AAGGCTGTTT TGGATAACTG CAGCAAAATG 
TTGCAAACAG CCCAGACTGT TGTTCGTGGC ATTGAGGAAA AGTGTGGCAG CAATCGGGTC 
AGAACCATGT CTGGCAGCCG CCCACCTCTT CTCTCACGTC TCTCTGAAAA CTCTGGAGAT 
GACATGAGTG ACATGGCGTT TGATTCCTTA CCTAGCTCAC CTTCCCCAGT CACCCCCCAC 
GGCCATCGAT TCGATTCGCA TGGTTGGCCT ATTTTTAATG AGGTTGACAA CTTACTTAAC 
GAAGAAAACA ATAACTTGGA CAATCATAGA GACTCTGAAA ATGGAGGGGA TAGTGGATGT 
CCCAGTGAAA AGAGAAGTGA ATCTGATGAG CTGGAACACA GAATCCATAA CGTCGGCAGG 
AGACGTTTTG AGTCAGAATC CGAAGACAGT TTTGGAAAGG TCAGTGTGGA AAAATGGAAC 
CAGTTCCACG CTTCCCGAGT TCATGTCCAC AGACCCTCTT GTGTGGCGTT TGAAGTAGAA 
AGACTTAATG CTCTGGAACT AGAGAAGAAA ATCGGGAAAT CGATTCTAGG AAAGGTTCAC 
TTGGCCATGG TGCGTTATCA TGAAGGGGGG CGTTTCTGCG AGAAAGACCA AGAATGGGAT 
CAGGAATCTG CCATTTTTCA TCTGGAACAC GCTGCTCACT GTGGGGAACT TGAAGCCATT 
ATTGGCCTTG GCCTCATGTA CTCACAGTCA CCCCATCACA TACTGACTGA TGTATCTGTC 
CAGGACACAG AAGATAATAG GATAAAAGGA TTTAACTATT TGTTGATGGC AGCAGAAGCA 
GGAGACCGTC CTTCCATGAT TCACGTTGCC CGAGCTTTTG ATACTGGTGT GAACCTTGGT 
TCAGACAGGA TAAAGGACTG GCAAGAAGCC CTCTTCTGGT ACGATTCTGC ACTCAACATG 
ACAGATTATG ATGAAGTTGG TGAATATGAT GGAACCCAAG ATGAACCCAA ATACTTGCTG 
CTGGCTAGGG AAGCAGAAAT GTTACTTACT GGGGGATTTA ACCTTCAGAA GGACCCACAG 
CGATCTGGTG ACTTGTACAC AGAAGCTGCT GAAGCAGCAA TGGAAGCAAT GAAAGGGAGG 
TTGGCCAATC AGTACTACCA GAAGGCAGAG GAGGCCTGGG CGTGTATGGA GGAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OXa31610
Clone ID Related Accession (Same CDS sequence) XM_012970072.2
Accession Version XM_012970072.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2265bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-09-07
Organism Xenopus tropicalis(tropical clawed frog)
Product eukaryotic elongation factor 2 kinase isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970072.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGCAGACG AAGATCTCAT CTTTAACATG GAGGGCCTTG ATAACATCGA CTTGGCCAAA 
GACCGATGCT CAGATTACAA CAAGGCCGAC AGCGATGAGG AAGACAATTT CTACATCTGC 
CCTATCACCG ACGACCCAGC TGCGCAGCAC CAGACCAAAA AGAAAAGTCC ATGTTTCTTT 
ACCAACTTGG TGAAAAAGAC AGAATATGGA ACTAGTTCCT CCCCTCGGAA CAGTTTTAAG 
AATGGACATT CCAAATATTC TAAGCATGGC TGCTTGGCTG AACGAGGTCG GGAGGCCTGG 
AAGCATGCCA TTCAGAAAGC TAAGCAGATG CCTGATCCAT GGGCTGAGTT TCACTTGGAA 
GACATTGAGA CAGAGCATGC TATCAGGCAC AGGTACAATG CTGTATCTGG CCAGTGGGTT 
CAAGATGATG TACTAATTAA AATGGCCTCT CAGGCGTTTG GCAGGGGAGC CATGAGAGAA 
TGTTACCGAA CGAAAAAACT CTCCAACTTC TCACACAAAC AGCAGTGGAA GAGCGCATAT 
AATTATGTGT CCAAACGATA CTTAGAGACT GTGGACAGAG ATGTGTACTT TGAGGACGTC 
CGACTCCAGA TGGAGGCAAA GCTGTGGGGA GAGGAATACA ACAGACACAA ACCACCAAAA 
CAGGTGGATA TCATGCAAAT GTGCATTATT GAAATGAAGG ACAGGCCTGG CAAACCGCTT 
TTTCACCTTG AACATTACAT CGAGGGAAAG TATATTAAAT ATAACTCAAA TTCTGGGTTT 
GTACGAGATG ACAATATCCG CCTCACTCCT CAGGCCTTCA GTCACTTCAC ATTTGAGCGC 
TCAGGACATC AGCTGATTGT TGTTGATGTA CAAGGAGTTG GTGATTTATA CACTGATCCT 
CAGATCCACA CAGAGAGTGG GACGGATTTT GGAGATGGAA ATTTAGGGGT ACGAGGGATG 
GCTCTGTTTT TCCATTCTCA TGCTTGCAAC AGAATTTGCA AAAGCATGGG ACTGACACCC 
TTTGACATTT CGGCCAAAGA AAAGGCTGTT TTGGATAACT GCAGCAAAAT GTTGCAAACA 
GCCCAGACTG TTGTTCGTGG CATTGAGGAA AAGTGTGGCA GCAATCGGGT CAGAACCATG 
TCTGGCAGCC GCCCACCTCT TCTCTCACGT CTCTCTGAAA ACTCTGGAGA TGACATGAGT 
GACATGGCGT TTGATTCCTT ACCTAGCTCA CCTTCCCCAG TCACCCCCCA CGGCCATCGA 
TTCGATTCGC ATGGTTGGCC TATTTTTAAT GAGGTTGACA ACTTACTTAA CGAAGAAAAC 
AATAACTTGG ACAATCATAG AGACTCTGAA AATGGAGGGG ATAGTGGATG TCCCAGTGAA 
AAGAGAAGTG AATCTGATGA GCTGGAACAC AGAATCCATA ACGTCGGCAG GAGACGTTTT 
GAGTCAGAAT CCGAAGACAG TTTTGGAAAG AGCAAAGGTG TAAATAAGGC CTATGTCATC 
CTTAAAAACA GTCCAAGGAG TAAACTGGTC AGTGTGGAAA AATGGAACCA GTTCCACGCT 
TCCCGAGTTC ATGTCCACAG ACCCTCTTGT GTGGCGTTTG AAGTAGAAAG ACTTAATGCT 
CTGGAACTAG AGAAGAAAAT CGGGAAATCG ATTCTAGGAA AGGTTCACTT GGCCATGGTG 
CGTTATCATG AAGGGGGGCG TTTCTGCGAG AAAGACCAAG AATGGGATCA GGAATCTGCC 
ATTTTTCATC TGGAACACGC TGCTCACTGT GGGGAACTTG AAGCCATTAT TGGCCTTGGC 
CTCATGTACT CACAGTCACC CCATCACATA CTGACTGATG TATCTGTCCA GGACACAGAA 
GATAATAGGA TAAAAGGATT TAACTATTTG TTGATGGCAG CAGAAGCAGG AGACCGTCCT 
TCCATGATTC ACGTTGCCCG AGCTTTTGAT ACTGGTGTGA ACCTTGGTTC AGACAGGATA 
AAGGACTGGC AAGAAGCCCT CTTCTGGTAC GATTCTGCAC TCAACATGAC AGATTATGAT 
GAAGGTGGTG AATATGATGG AACCCAAGAT GAACCCAAAT ACTTGCTGCT GGCTAGGGAA 
GCAGAAATGT TACTTACTGG GGGATTTAAC CTTCAGAAGG ACCCACAGCG ATCTGGTGAC 
TTGTACACAG AAGCTGCTGA AGCAGCAATG GAAGCAATGA AAGGGAGGTT GGCCAATCAG 
TACTACCAGA AGGCAGAGGA GGCCTGGGCG TGTATGGAGG AGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_012825526.1
CDS335..2599
Translation

Target ORF information:

RefSeq Version XM_012970072.2
Organism Xenopus tropicalis(tropical clawed frog)
Definition Xenopus tropicalis eukaryotic elongation factor 2 kinase (eef2k), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_012970072.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGCAGACG AAGATCTCAT CTTTAACATG GAGGGCCTTG ATAACATCGA CTTGGCCAAA 
GACCGATGCT CAGATTACAA CAAGGCCGAC AGCGATGAGG AAGACAATTT CTACATCTGC 
CCTATCACCG ACGACCCAGC TGCGCAGCAC CAGACCAAAA AGAAAAGTCC ATGTTTCTTT 
ACCAACTTGG TGAAAAAGAC AGAATATGGA ACTAGTTCCT CCCCTCGGAA CAGTTTTAAG 
AATGGACATT CCAAATATTC TAAGCATGGC TGCTTGGCTG AACGAGGTCG GGAGGCCTGG 
AAGCATGCCA TTCAGAAAGC TAAGCAGATG CCTGATCCAT GGGCTGAGTT TCACTTGGAA 
GACATTGAGA CAGAGCATGC TATCAGGCAC AGGTACAATG CTGTATCTGG CCAGTGGGTT 
CAAGATGATG TACTAATTAA AATGGCCTCT CAGGCGTTTG GCAGGGGAGC CATGAGAGAA 
TGTTACCGAA CGAAAAAACT CTCCAACTTC TCACACAAAC AGCAGTGGAA GAGCGCATAT 
AATTATGTGT CCAAACGATA CTTAGAGACT GTGGACAGAG ATGTGTACTT TGAGGACGTC 
CGACTCCAGA TGGAGGCAAA GCTGTGGGGA GAGGAATACA ACAGACACAA ACCACCAAAA 
CAGGTGGATA TCATGCAAAT GTGCATTATT GAAATGAAGG ACAGGCCTGG CAAACCGCTT 
TTTCACCTTG AACATTACAT CGAGGGAAAG TATATTAAAT ATAACTCAAA TTCTGGGTTT 
GTACGAGATG ACAATATCCG CCTCACTCCT CAGGCCTTCA GTCACTTCAC ATTTGAGCGC 
TCAGGACATC AGCTGATTGT TGTTGATGTA CAAGGAGTTG GTGATTTATA CACTGATCCT 
CAGATCCACA CAGAGAGTGG GACGGATTTT GGAGATGGAA ATTTAGGGGT ACGAGGGATG 
GCTCTGTTTT TCCATTCTCA TGCTTGCAAC AGAATTTGCA AAAGCATGGG ACTGACACCC 
TTTGACATTT CGGCCAAAGA AAAGGCTGTT TTGGATAACT GCAGCAAAAT GTTGCAAACA 
GCCCAGACTG TTGTTCGTGG CATTGAGGAA AAGTGTGGCA GCAATCGGGT CAGAACCATG 
TCTGGCAGCC GCCCACCTCT TCTCTCACGT CTCTCTGAAA ACTCTGGAGA TGACATGAGT 
GACATGGCGT TTGATTCCTT ACCTAGCTCA CCTTCCCCAG TCACCCCCCA CGGCCATCGA 
TTCGATTCGC ATGGTTGGCC TATTTTTAAT GAGGTTGACA ACTTACTTAA CGAAGAAAAC 
AATAACTTGG ACAATCATAG AGACTCTGAA AATGGAGGGG ATAGTGGATG TCCCAGTGAA 
AAGAGAAGTG AATCTGATGA GCTGGAACAC AGAATCCATA ACGTCGGCAG GAGACGTTTT 
GAGTCAGAAT CCGAAGACAG TTTTGGAAAG AGCAAAGGTG TAAATAAGGC CTATGTCATC 
CTTAAAAACA GTCCAAGGAG TAAACTGGTC AGTGTGGAAA AATGGAACCA GTTCCACGCT 
TCCCGAGTTC ATGTCCACAG ACCCTCTTGT GTGGCGTTTG AAGTAGAAAG ACTTAATGCT 
CTGGAACTAG AGAAGAAAAT CGGGAAATCG ATTCTAGGAA AGGTTCACTT GGCCATGGTG 
CGTTATCATG AAGGGGGGCG TTTCTGCGAG AAAGACCAAG AATGGGATCA GGAATCTGCC 
ATTTTTCATC TGGAACACGC TGCTCACTGT GGGGAACTTG AAGCCATTAT TGGCCTTGGC 
CTCATGTACT CACAGTCACC CCATCACATA CTGACTGATG TATCTGTCCA GGACACAGAA 
GATAATAGGA TAAAAGGATT TAACTATTTG TTGATGGCAG CAGAAGCAGG AGACCGTCCT 
TCCATGATTC ACGTTGCCCG AGCTTTTGAT ACTGGTGTGA ACCTTGGTTC AGACAGGATA 
AAGGACTGGC AAGAAGCCCT CTTCTGGTAC GATTCTGCAC TCAACATGAC AGATTATGAT 
GAAGGTGGTG AATATGATGG AACCCAAGAT GAACCCAAAT ACTTGCTGCT GGCTAGGGAA 
GCAGAAATGT TACTTACTGG GGGATTTAAC CTTCAGAAGG ACCCACAGCG ATCTGGTGAC 
TTGTACACAG AAGCTGCTGA AGCAGCAATG GAAGCAATGA AAGGGAGGTT GGCCAATCAG 
TACTACCAGA AGGCAGAGGA GGCCTGGGCG TGTATGGAGG AGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OXa31611
Clone ID Related Accession (Same CDS sequence) XM_012970073.2
Accession Version XM_012970073.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2214bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-09-07
Organism Xenopus tropicalis(tropical clawed frog)
Product eukaryotic elongation factor 2 kinase isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970073.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCAGACG AAGATCTCAT CTTTAACATG GAGGGCCTTG ATAACATCGA CTTGGCCAAA 
GACCGATGCT CAGATTACAA CAAGGCCGAC AGCGATGAGG AAGACAATTT CTACATCTGC 
CCTATCACCG ACGACCCAGC TGCGCAGCAC CAGACCAAAA AGAAAAGTCC ATGTTTCTTT 
ACCAACTTGG TGAAAAAGAC AGAATATGGA ACTAGTTCCT CCCCTCGGAA CAGTTTTAAG 
GAGGCCTGGA AGCATGCCAT TCAGAAAGCT AAGCAGATGC CTGATCCATG GGCTGAGTTT 
CACTTGGAAG ACATTGAGAC AGAGCATGCT ATCAGGCACA GGTACAATGC TGTATCTGGC 
CAGTGGGTTC AAGATGATGT ACTAATTAAA ATGGCCTCTC AGGCGTTTGG CAGGGGAGCC 
ATGAGAGAAT GTTACCGAAC GAAAAAACTC TCCAACTTCT CACACAAACA GCAGTGGAAG 
AGCGCATATA ATTATGTGTC CAAACGATAC TTAGAGACTG TGGACAGAGA TGTGTACTTT 
GAGGACGTCC GACTCCAGAT GGAGGCAAAG CTGTGGGGAG AGGAATACAA CAGACACAAA 
CCACCAAAAC AGGTGGATAT CATGCAAATG TGCATTATTG AAATGAAGGA CAGGCCTGGC 
AAACCGCTTT TTCACCTTGA ACATTACATC GAGGGAAAGT ATATTAAATA TAACTCAAAT 
TCTGGGTTTG TACGAGATGA CAATATCCGC CTCACTCCTC AGGCCTTCAG TCACTTCACA 
TTTGAGCGCT CAGGACATCA GCTGATTGTT GTTGATGTAC AAGGAGTTGG TGATTTATAC 
ACTGATCCTC AGATCCACAC AGAGAGTGGG ACGGATTTTG GAGATGGAAA TTTAGGGGTA 
CGAGGGATGG CTCTGTTTTT CCATTCTCAT GCTTGCAACA GAATTTGCAA AAGCATGGGA 
CTGACACCCT TTGACATTTC GGCCAAAGAA AAGGCTGTTT TGGATAACTG CAGCAAAATG 
TTGCAAACAG CCCAGACTGT TGTTCGTGGC ATTGAGGAAA AGTGTGGCAG CAATCGGGTC 
AGAACCATGT CTGGCAGCCG CCCACCTCTT CTCTCACGTC TCTCTGAAAA CTCTGGAGAT 
GACATGAGTG ACATGGCGTT TGATTCCTTA CCTAGCTCAC CTTCCCCAGT CACCCCCCAC 
GGCCATCGAT TCGATTCGCA TGGTTGGCCT ATTTTTAATG AGGTTGACAA CTTACTTAAC 
GAAGAAAACA ATAACTTGGA CAATCATAGA GACTCTGAAA ATGGAGGGGA TAGTGGATGT 
CCCAGTGAAA AGAGAAGTGA ATCTGATGAG CTGGAACACA GAATCCATAA CGTCGGCAGG 
AGACGTTTTG AGTCAGAATC CGAAGACAGT TTTGGAAAGA GCAAAGGTGT AAATAAGGCC 
TATGTCATCC TTAAAAACAG TCCAAGGAGT AAACTGGTCA GTGTGGAAAA ATGGAACCAG 
TTCCACGCTT CCCGAGTTCA TGTCCACAGA CCCTCTTGTG TGGCGTTTGA AGTAGAAAGA 
CTTAATGCTC TGGAACTAGA GAAGAAAATC GGGAAATCGA TTCTAGGAAA GGTTCACTTG 
GCCATGGTGC GTTATCATGA AGGGGGGCGT TTCTGCGAGA AAGACCAAGA ATGGGATCAG 
GAATCTGCCA TTTTTCATCT GGAACACGCT GCTCACTGTG GGGAACTTGA AGCCATTATT 
GGCCTTGGCC TCATGTACTC ACAGTCACCC CATCACATAC TGACTGATGT ATCTGTCCAG 
GACACAGAAG ATAATAGGAT AAAAGGATTT AACTATTTGT TGATGGCAGC AGAAGCAGGA 
GACCGTCCTT CCATGATTCA CGTTGCCCGA GCTTTTGATA CTGGTGTGAA CCTTGGTTCA 
GACAGGATAA AGGACTGGCA AGAAGCCCTC TTCTGGTACG ATTCTGCACT CAACATGACA 
GATTATGATG AAGGTGGTGA ATATGATGGA ACCCAAGATG AACCCAAATA CTTGCTGCTG 
GCTAGGGAAG CAGAAATGTT ACTTACTGGG GGATTTAACC TTCAGAAGGA CCCACAGCGA 
TCTGGTGACT TGTACACAGA AGCTGCTGAA GCAGCAATGG AAGCAATGAA AGGGAGGTTG 
GCCAATCAGT ACTACCAGAA GGCAGAGGAG GCCTGGGCGT GTATGGAGGA GTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_012825527.1
CDS334..2547
Translation

Target ORF information:

RefSeq Version XM_012970073.2
Organism Xenopus tropicalis(tropical clawed frog)
Definition Xenopus tropicalis eukaryotic elongation factor 2 kinase (eef2k), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_012970073.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCAGACG AAGATCTCAT CTTTAACATG GAGGGCCTTG ATAACATCGA CTTGGCCAAA 
GACCGATGCT CAGATTACAA CAAGGCCGAC AGCGATGAGG AAGACAATTT CTACATCTGC 
CCTATCACCG ACGACCCAGC TGCGCAGCAC CAGACCAAAA AGAAAAGTCC ATGTTTCTTT 
ACCAACTTGG TGAAAAAGAC AGAATATGGA ACTAGTTCCT CCCCTCGGAA CAGTTTTAAG 
GAGGCCTGGA AGCATGCCAT TCAGAAAGCT AAGCAGATGC CTGATCCATG GGCTGAGTTT 
CACTTGGAAG ACATTGAGAC AGAGCATGCT ATCAGGCACA GGTACAATGC TGTATCTGGC 
CAGTGGGTTC AAGATGATGT ACTAATTAAA ATGGCCTCTC AGGCGTTTGG CAGGGGAGCC 
ATGAGAGAAT GTTACCGAAC GAAAAAACTC TCCAACTTCT CACACAAACA GCAGTGGAAG 
AGCGCATATA ATTATGTGTC CAAACGATAC TTAGAGACTG TGGACAGAGA TGTGTACTTT 
GAGGACGTCC GACTCCAGAT GGAGGCAAAG CTGTGGGGAG AGGAATACAA CAGACACAAA 
CCACCAAAAC AGGTGGATAT CATGCAAATG TGCATTATTG AAATGAAGGA CAGGCCTGGC 
AAACCGCTTT TTCACCTTGA ACATTACATC GAGGGAAAGT ATATTAAATA TAACTCAAAT 
TCTGGGTTTG TACGAGATGA CAATATCCGC CTCACTCCTC AGGCCTTCAG TCACTTCACA 
TTTGAGCGCT CAGGACATCA GCTGATTGTT GTTGATGTAC AAGGAGTTGG TGATTTATAC 
ACTGATCCTC AGATCCACAC AGAGAGTGGG ACGGATTTTG GAGATGGAAA TTTAGGGGTA 
CGAGGGATGG CTCTGTTTTT CCATTCTCAT GCTTGCAACA GAATTTGCAA AAGCATGGGA 
CTGACACCCT TTGACATTTC GGCCAAAGAA AAGGCTGTTT TGGATAACTG CAGCAAAATG 
TTGCAAACAG CCCAGACTGT TGTTCGTGGC ATTGAGGAAA AGTGTGGCAG CAATCGGGTC 
AGAACCATGT CTGGCAGCCG CCCACCTCTT CTCTCACGTC TCTCTGAAAA CTCTGGAGAT 
GACATGAGTG ACATGGCGTT TGATTCCTTA CCTAGCTCAC CTTCCCCAGT CACCCCCCAC 
GGCCATCGAT TCGATTCGCA TGGTTGGCCT ATTTTTAATG AGGTTGACAA CTTACTTAAC 
GAAGAAAACA ATAACTTGGA CAATCATAGA GACTCTGAAA ATGGAGGGGA TAGTGGATGT 
CCCAGTGAAA AGAGAAGTGA ATCTGATGAG CTGGAACACA GAATCCATAA CGTCGGCAGG 
AGACGTTTTG AGTCAGAATC CGAAGACAGT TTTGGAAAGA GCAAAGGTGT AAATAAGGCC 
TATGTCATCC TTAAAAACAG TCCAAGGAGT AAACTGGTCA GTGTGGAAAA ATGGAACCAG 
TTCCACGCTT CCCGAGTTCA TGTCCACAGA CCCTCTTGTG TGGCGTTTGA AGTAGAAAGA 
CTTAATGCTC TGGAACTAGA GAAGAAAATC GGGAAATCGA TTCTAGGAAA GGTTCACTTG 
GCCATGGTGC GTTATCATGA AGGGGGGCGT TTCTGCGAGA AAGACCAAGA ATGGGATCAG 
GAATCTGCCA TTTTTCATCT GGAACACGCT GCTCACTGTG GGGAACTTGA AGCCATTATT 
GGCCTTGGCC TCATGTACTC ACAGTCACCC CATCACATAC TGACTGATGT ATCTGTCCAG 
GACACAGAAG ATAATAGGAT AAAAGGATTT AACTATTTGT TGATGGCAGC AGAAGCAGGA 
GACCGTCCTT CCATGATTCA CGTTGCCCGA GCTTTTGATA CTGGTGTGAA CCTTGGTTCA 
GACAGGATAA AGGACTGGCA AGAAGCCCTC TTCTGGTACG ATTCTGCACT CAACATGACA 
GATTATGATG AAGGTGGTGA ATATGATGGA ACCCAAGATG AACCCAAATA CTTGCTGCTG 
GCTAGGGAAG CAGAAATGTT ACTTACTGGG GGATTTAACC TTCAGAAGGA CCCACAGCGA 
TCTGGTGACT TGTACACAGA AGCTGCTGAA GCAGCAATGG AAGCAATGAA AGGGAGGTTG 
GCCAATCAGT ACTACCAGAA GGCAGAGGAG GCCTGGGCGT GTATGGAGGA GTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OXa31612
Clone ID Related Accession (Same CDS sequence) XM_012970074.2
Accession Version XM_012970074.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2208bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-09-07
Organism Xenopus tropicalis(tropical clawed frog)
Product eukaryotic elongation factor 2 kinase isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970074.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCAGACG AAGATCTCAT CTTTAACATG GAGGGCCTTG ATAACATCGA CTTGGCCAAA 
GACCGATGCT CAGATTACAA CAAGGCCGAC AGCGATGAGG AAGACAATTT CTACATCTGC 
CCTATCACCG ACGACCCAGC TGCGCAGCAC CAGACCAAAA AGAAAAGTCC ATGTTTCTTT 
ACCAACTTGG TGAAAAAGAC AGAATATGGA ACTAGTTCCT CCCCTCGGAA CAGTTTTAAG 
AATGGACATT CCAAATATTC TAAGCATGGC TGCTTGGCTG AACGAGGTCG GGAGGCCTGG 
AAGCATGCCA TTCAGAAAGC TAAGCAGATG CCTGATCCAT GGGCTGAGTT TCACTTGGAA 
GACATTGAGA CAGAGCATGC TATCAGGCAC AGGTACAATG CTGTATCTGG CCAGTGGGTT 
CAAGATGATG TACTAATTAA AATGGCCTCT CAGGCGTTTG GCAGGGGAGC CATGAGAGAA 
TGTTACCGAA CGAAAAAACT CTCCAACTTC TCACACAAAC AGCAGTGGAA GAGCGCATAT 
AATTATGTGT CCAAACGATA CTTAGAGACT GTGGACAGAG ATGTGTACTT TGAGGACGTC 
CGACTCCAGA TGGAGGCAAA GCTGTGGGGA GAGGAATACA ACAGACACAA ACCACCAAAA 
CAGGTGGATA TCATGCAAAT GTGCATTATT GAAATGAAGG ACAGGCCTGG CAAACCGCTT 
TTTCACCTTG AACATTACAT CGAGGGAAAG TATATTAAAT ATAACTCAAA TTCTGGGTTT 
GTACGAGATG ACAATATCCG CCTCACTCCT CAGGCCTTCA GTCACTTCAC ATTTGAGCGC 
TCAGGACATC AGCTGATTGT TGTTGATGTA CAAGGAGTTG GTGATTTATA CACTGATCCT 
CAGATCCACA CAGAGAGTGG GACGGATTTT GGAGATGGAA ATTTAGGGGT ACGAGGGATG 
GCTCTGTTTT TCCATTCTCA TGCTTGCAAC AGAATTTGCA AAAGCATGGG ACTGACACCC 
TTTGACATTT CGGCCAAAGA AAAGGCTGTT TTGGATAACT GCAGCAAAAT GTTGCAAACA 
GCCCAGACTG TTGTTCGTGG CATTGAGGAA AAGTGTGGCA GCAATCGGGT CAGAACCATG 
TCTGGCAGCC GCCCACCTCT TCTCTCACGT CTCTCTGAAA ACTCTGGAGA TGACATGAGT 
GACATGGCGT TTGATTCCTT ACCTAGCTCA CCTTCCCCAG TCACCCCCCA CGGCCATCGA 
TTCGATTCGC ATGGTTGGCC TATTTTTAAT GAGGTTGACA ACTTACTTAA CGAAGAAAAC 
AATAACTTGG ACAATCATAG AGACTCTGAA AATGGAGGGG ATAGTGGATG TCCCAGTGAA 
AAGAGAAGTG AATCTGATGA GCTGGAACAC AGAATCCATA ACGTCGGCAG GAGACGTTTT 
GAGTCAGAAT CCGAAGACAG TTTTGGAAAG GTCAGTGTGG AAAAATGGAA CCAGTTCCAC 
GCTTCCCGAG TTCATGTCCA CAGACCCTCT TGTGTGGCGT TTGAAGTAGA AAGACTTAAT 
GCTCTGGAAC TAGAGAAGAA AATCGGGAAA TCGATTCTAG GAAAGGTTCA CTTGGCCATG 
GTGCGTTATC ATGAAGGGGG GCGTTTCTGC GAGAAAGACC AAGAATGGGA TCAGGAATCT 
GCCATTTTTC ATCTGGAACA CGCTGCTCAC TGTGGGGAAC TTGAAGCCAT TATTGGCCTT 
GGCCTCATGT ACTCACAGTC ACCCCATCAC ATACTGACTG ATGTATCTGT CCAGGACACA 
GAAGATAATA GGATAAAAGG ATTTAACTAT TTGTTGATGG CAGCAGAAGC AGGAGACCGT 
CCTTCCATGA TTCACGTTGC CCGAGCTTTT GATACTGGTG TGAACCTTGG TTCAGACAGG 
ATAAAGGACT GGCAAGAAGC CCTCTTCTGG TACGATTCTG CACTCAACAT GACAGATTAT 
GATGAAGGTG GTGAATATGA TGGAACCCAA GATGAACCCA AATACTTGCT GCTGGCTAGG 
GAAGCAGAAA TGTTACTTAC TGGGGGATTT AACCTTCAGA AGGACCCACA GCGATCTGGT 
GACTTGTACA CAGAAGCTGC TGAAGCAGCA ATGGAAGCAA TGAAAGGGAG GTTGGCCAAT 
CAGTACTACC AGAAGGCAGA GGAGGCCTGG GCGTGTATGG AGGAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_012825528.1
CDS334..2541
Translation

Target ORF information:

RefSeq Version XM_012970074.2
Organism Xenopus tropicalis(tropical clawed frog)
Definition Xenopus tropicalis eukaryotic elongation factor 2 kinase (eef2k), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_012970074.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCAGACG AAGATCTCAT CTTTAACATG GAGGGCCTTG ATAACATCGA CTTGGCCAAA 
GACCGATGCT CAGATTACAA CAAGGCCGAC AGCGATGAGG AAGACAATTT CTACATCTGC 
CCTATCACCG ACGACCCAGC TGCGCAGCAC CAGACCAAAA AGAAAAGTCC ATGTTTCTTT 
ACCAACTTGG TGAAAAAGAC AGAATATGGA ACTAGTTCCT CCCCTCGGAA CAGTTTTAAG 
AATGGACATT CCAAATATTC TAAGCATGGC TGCTTGGCTG AACGAGGTCG GGAGGCCTGG 
AAGCATGCCA TTCAGAAAGC TAAGCAGATG CCTGATCCAT GGGCTGAGTT TCACTTGGAA 
GACATTGAGA CAGAGCATGC TATCAGGCAC AGGTACAATG CTGTATCTGG CCAGTGGGTT 
CAAGATGATG TACTAATTAA AATGGCCTCT CAGGCGTTTG GCAGGGGAGC CATGAGAGAA 
TGTTACCGAA CGAAAAAACT CTCCAACTTC TCACACAAAC AGCAGTGGAA GAGCGCATAT 
AATTATGTGT CCAAACGATA CTTAGAGACT GTGGACAGAG ATGTGTACTT TGAGGACGTC 
CGACTCCAGA TGGAGGCAAA GCTGTGGGGA GAGGAATACA ACAGACACAA ACCACCAAAA 
CAGGTGGATA TCATGCAAAT GTGCATTATT GAAATGAAGG ACAGGCCTGG CAAACCGCTT 
TTTCACCTTG AACATTACAT CGAGGGAAAG TATATTAAAT ATAACTCAAA TTCTGGGTTT 
GTACGAGATG ACAATATCCG CCTCACTCCT CAGGCCTTCA GTCACTTCAC ATTTGAGCGC 
TCAGGACATC AGCTGATTGT TGTTGATGTA CAAGGAGTTG GTGATTTATA CACTGATCCT 
CAGATCCACA CAGAGAGTGG GACGGATTTT GGAGATGGAA ATTTAGGGGT ACGAGGGATG 
GCTCTGTTTT TCCATTCTCA TGCTTGCAAC AGAATTTGCA AAAGCATGGG ACTGACACCC 
TTTGACATTT CGGCCAAAGA AAAGGCTGTT TTGGATAACT GCAGCAAAAT GTTGCAAACA 
GCCCAGACTG TTGTTCGTGG CATTGAGGAA AAGTGTGGCA GCAATCGGGT CAGAACCATG 
TCTGGCAGCC GCCCACCTCT TCTCTCACGT CTCTCTGAAA ACTCTGGAGA TGACATGAGT 
GACATGGCGT TTGATTCCTT ACCTAGCTCA CCTTCCCCAG TCACCCCCCA CGGCCATCGA 
TTCGATTCGC ATGGTTGGCC TATTTTTAAT GAGGTTGACA ACTTACTTAA CGAAGAAAAC 
AATAACTTGG ACAATCATAG AGACTCTGAA AATGGAGGGG ATAGTGGATG TCCCAGTGAA 
AAGAGAAGTG AATCTGATGA GCTGGAACAC AGAATCCATA ACGTCGGCAG GAGACGTTTT 
GAGTCAGAAT CCGAAGACAG TTTTGGAAAG GTCAGTGTGG AAAAATGGAA CCAGTTCCAC 
GCTTCCCGAG TTCATGTCCA CAGACCCTCT TGTGTGGCGT TTGAAGTAGA AAGACTTAAT 
GCTCTGGAAC TAGAGAAGAA AATCGGGAAA TCGATTCTAG GAAAGGTTCA CTTGGCCATG 
GTGCGTTATC ATGAAGGGGG GCGTTTCTGC GAGAAAGACC AAGAATGGGA TCAGGAATCT 
GCCATTTTTC ATCTGGAACA CGCTGCTCAC TGTGGGGAAC TTGAAGCCAT TATTGGCCTT 
GGCCTCATGT ACTCACAGTC ACCCCATCAC ATACTGACTG ATGTATCTGT CCAGGACACA 
GAAGATAATA GGATAAAAGG ATTTAACTAT TTGTTGATGG CAGCAGAAGC AGGAGACCGT 
CCTTCCATGA TTCACGTTGC CCGAGCTTTT GATACTGGTG TGAACCTTGG TTCAGACAGG 
ATAAAGGACT GGCAAGAAGC CCTCTTCTGG TACGATTCTG CACTCAACAT GACAGATTAT 
GATGAAGGTG GTGAATATGA TGGAACCCAA GATGAACCCA AATACTTGCT GCTGGCTAGG 
GAAGCAGAAA TGTTACTTAC TGGGGGATTT AACCTTCAGA AGGACCCACA GCGATCTGGT 
GACTTGTACA CAGAAGCTGC TGAAGCAGCA ATGGAAGCAA TGAAAGGGAG GTTGGCCAAT 
CAGTACTACC AGAAGGCAGA GGAGGCCTGG GCGTGTATGG AGGAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OXa65869
Clone ID Related Accession (Same CDS sequence) XM_012970072.3
Accession Version XM_012970072.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2265bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-17
Organism Xenopus tropicalis(tropical clawed frog)
Product eukaryotic elongation factor 2 kinase isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970072.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGCAGACG AAGATCTCAT CTTTAACATG GAGGGCCTTG ATAACATCGA CTTGGCCAAA 
GACCGATGCT CAGATTACAA CAAGGCCGAC AGCGATGAGG AAGACAATTT CTACATCTGC 
CCTATCACCG ACGACCCAGC TGCGCAGCAC CAGACCAAAA AGAAAAGTCC ATGTTTCTTT 
ACCAACTTGG TGAAAAAGAC AGAATATGGA ACTAGTTCCT CCCCTCGGAA CAGTTTTAAG 
AATGGACATT CCAAATATTC TAAGCATGGC TGCTTGGCTG AACGAGGTCG GGAGGCCTGG 
AAGCATGCCA TTCAGAAAGC TAAGCAGATG CCTGATCCAT GGGCTGAGTT TCACTTGGAA 
GACATTGAGA CAGAGCATGC TATCAGGCAC AGGTACAATG CTGTATCTGG CCAGTGGGTT 
CAAGATGATG TACTAATTAA AATGGCCTCT CAGGCGTTTG GCAGGGGAGC CATGAGAGAA 
TGTTACCGAA CGAAAAAACT CTCCAACTTC TCACACAAAC AGCAGTGGAA GAGCGCATAT 
AATTATGTGT CCAAACGATA CTTAGAGACT GTGGACAGAG ATGTGTACTT TGAGGACGTC 
CGACTCCAGA TGGAGGCAAA GCTGTGGGGA GAGGAATACA ACAGACACAA ACCACCAAAA 
CAGGTGGATA TCATGCAAAT GTGCATTATT GAAATGAAGG ACAGGCCTGG CAAACCGCTT 
TTTCACCTTG AACATTACAT CGAGGGAAAG TATATTAAAT ATAACTCAAA TTCTGGGTTT 
GTACGAGATG ACAATATCCG CCTCACTCCT CAGGCCTTCA GTCACTTCAC ATTTGAGCGC 
TCAGGACATC AGCTGATTGT TGTTGATGTA CAAGGAGTTG GTGATTTATA CACTGATCCT 
CAGATCCACA CAGAGAGTGG GACGGATTTT GGAGATGGAA ATTTAGGGGT ACGAGGGATG 
GCTCTGTTTT TCCATTCTCA TGCTTGCAAC AGAATTTGCA AAAGCATGGG ACTGACACCC 
TTTGACATTT CGGCCAAAGA AAAGGCTGTT TTGGATAACT GCAGCAAAAT GTTGCAAACA 
GCCCAGACTG TTGTTCGTGG CATTGAGGAA AAGTGTGGCA GCAATCGGGT CAGAACCATG 
TCTGGCAGCC GCCCACCTCT TCTCTCACGT CTCTCTGAAA ACTCTGGAGA TGACATGAGT 
GACATGGCGT TCGATTCCTT ACCTAGCTCA CCTTCCCCAG TCACCCCCCA CGGCCATCGA 
TTCGATTCGC ATGGTTGGCC TATTTTTAAT GAGGTCGACA ACTTACTTAA CGAAGAAAAC 
AATAACTTGG ACAATCATAG AGACTCTGAA AATGGAGGGG ATAGTGGATG TCCCAGTGAA 
AAGAGAAGTG AATCTGATGA GCTGGAACAC AGAATCCATA ACGTCGGCAG GAGACGTTTT 
GAGTCAGAAT CCGAAGACAG TTTTGGAAAG AGCAAAGGTG TAAATAAGGC CTATGTCATC 
CTTAAAAACA GTCCAAGGAG TAAACTGGTC AGTGTGGAAA AATGGAACCA GTTCCACGCT 
TCCCGAGTTC ATGTCCACAG ACCCTCTTGT GTGGCGTTTG AAGTAGAAAG ACTTAATGCT 
CTGGAACTAG AGAAGAAAAT CGGGAAATCG ATTCTAGGAA AGGTTCACTT GGCCATGGTG 
CGTTATCATG AAGGGGGGCG TTTCTGCGAG AAAGACCAAG AATGGGATCA GGAATCTGCC 
ATTTTTCATC TGGAACACGC TGCTCACTGT GGGGAACTTG AAGCCATTAT TGGCCTTGGC 
CTCATGTACT CACAGTCACC CCATCACATA CTGACTGATG TATCTGTCCA GGACACAGAA 
GATAATAGGA TAAAAGGATT TAACTATTTG TTGATGGCAG CAGAAGCAGG AGACCGTCCT 
TCCATGATTC ACGTTGCCCG AGCTTTTGAT ACTGGTGTGA ACCTTGGTTC AGACAGGATA 
AAGGACTGGC AAGAAGCCCT CTTCTGGTAC GATTCTGCAC TCAACATGAC AGATTATGAT 
GAAGGTGGTG AATATGATGG AACCCAAGAT GAACCCAAAT ACTTGCTGCT GGCTAGGGAA 
GCAGAAATGT TACTTACTGG GGGATTTAAC CTTCAGAAGG ACCCACAGCG ATCTGGTGAC 
TTGTACACAG AAGCTGCTGA AGCAGCAATG GAAGCAATGA AAGGGAGGTT GGCCAATCAG 
TACTACCAGA AGGCAGAGGA GGCCTGGGCG TGTATGGAGG AGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_012825526.1
CDS306..2570
Translation

Target ORF information:

RefSeq Version XM_012970072.3
Organism Xenopus tropicalis(tropical clawed frog)
Definition Xenopus tropicalis eukaryotic elongation factor 2 kinase (eef2k), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_012970072.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGCAGACG AAGATCTCAT CTTTAACATG GAGGGCCTTG ATAACATCGA CTTGGCCAAA 
GACCGATGCT CAGATTACAA CAAGGCCGAC AGCGATGAGG AAGACAATTT CTACATCTGC 
CCTATCACCG ACGACCCAGC TGCGCAGCAC CAGACCAAAA AGAAAAGTCC ATGTTTCTTT 
ACCAACTTGG TGAAAAAGAC AGAATATGGA ACTAGTTCCT CCCCTCGGAA CAGTTTTAAG 
AATGGACATT CCAAATATTC TAAGCATGGC TGCTTGGCTG AACGAGGTCG GGAGGCCTGG 
AAGCATGCCA TTCAGAAAGC TAAGCAGATG CCTGATCCAT GGGCTGAGTT TCACTTGGAA 
GACATTGAGA CAGAGCATGC TATCAGGCAC AGGTACAATG CTGTATCTGG CCAGTGGGTT 
CAAGATGATG TACTAATTAA AATGGCCTCT CAGGCGTTTG GCAGGGGAGC CATGAGAGAA 
TGTTACCGAA CGAAAAAACT CTCCAACTTC TCACACAAAC AGCAGTGGAA GAGCGCATAT 
AATTATGTGT CCAAACGATA CTTAGAGACT GTGGACAGAG ATGTGTACTT TGAGGACGTC 
CGACTCCAGA TGGAGGCAAA GCTGTGGGGA GAGGAATACA ACAGACACAA ACCACCAAAA 
CAGGTGGATA TCATGCAAAT GTGCATTATT GAAATGAAGG ACAGGCCTGG CAAACCGCTT 
TTTCACCTTG AACATTACAT CGAGGGAAAG TATATTAAAT ATAACTCAAA TTCTGGGTTT 
GTACGAGATG ACAATATCCG CCTCACTCCT CAGGCCTTCA GTCACTTCAC ATTTGAGCGC 
TCAGGACATC AGCTGATTGT TGTTGATGTA CAAGGAGTTG GTGATTTATA CACTGATCCT 
CAGATCCACA CAGAGAGTGG GACGGATTTT GGAGATGGAA ATTTAGGGGT ACGAGGGATG 
GCTCTGTTTT TCCATTCTCA TGCTTGCAAC AGAATTTGCA AAAGCATGGG ACTGACACCC 
TTTGACATTT CGGCCAAAGA AAAGGCTGTT TTGGATAACT GCAGCAAAAT GTTGCAAACA 
GCCCAGACTG TTGTTCGTGG CATTGAGGAA AAGTGTGGCA GCAATCGGGT CAGAACCATG 
TCTGGCAGCC GCCCACCTCT TCTCTCACGT CTCTCTGAAA ACTCTGGAGA TGACATGAGT 
GACATGGCGT TCGATTCCTT ACCTAGCTCA CCTTCCCCAG TCACCCCCCA CGGCCATCGA 
TTCGATTCGC ATGGTTGGCC TATTTTTAAT GAGGTCGACA ACTTACTTAA CGAAGAAAAC 
AATAACTTGG ACAATCATAG AGACTCTGAA AATGGAGGGG ATAGTGGATG TCCCAGTGAA 
AAGAGAAGTG AATCTGATGA GCTGGAACAC AGAATCCATA ACGTCGGCAG GAGACGTTTT 
GAGTCAGAAT CCGAAGACAG TTTTGGAAAG AGCAAAGGTG TAAATAAGGC CTATGTCATC 
CTTAAAAACA GTCCAAGGAG TAAACTGGTC AGTGTGGAAA AATGGAACCA GTTCCACGCT 
TCCCGAGTTC ATGTCCACAG ACCCTCTTGT GTGGCGTTTG AAGTAGAAAG ACTTAATGCT 
CTGGAACTAG AGAAGAAAAT CGGGAAATCG ATTCTAGGAA AGGTTCACTT GGCCATGGTG 
CGTTATCATG AAGGGGGGCG TTTCTGCGAG AAAGACCAAG AATGGGATCA GGAATCTGCC 
ATTTTTCATC TGGAACACGC TGCTCACTGT GGGGAACTTG AAGCCATTAT TGGCCTTGGC 
CTCATGTACT CACAGTCACC CCATCACATA CTGACTGATG TATCTGTCCA GGACACAGAA 
GATAATAGGA TAAAAGGATT TAACTATTTG TTGATGGCAG CAGAAGCAGG AGACCGTCCT 
TCCATGATTC ACGTTGCCCG AGCTTTTGAT ACTGGTGTGA ACCTTGGTTC AGACAGGATA 
AAGGACTGGC AAGAAGCCCT CTTCTGGTAC GATTCTGCAC TCAACATGAC AGATTATGAT 
GAAGGTGGTG AATATGATGG AACCCAAGAT GAACCCAAAT ACTTGCTGCT GGCTAGGGAA 
GCAGAAATGT TACTTACTGG GGGATTTAAC CTTCAGAAGG ACCCACAGCG ATCTGGTGAC 
TTGTACACAG AAGCTGCTGA AGCAGCAATG GAAGCAATGA AAGGGAGGTT GGCCAATCAG 
TACTACCAGA AGGCAGAGGA GGCCTGGGCG TGTATGGAGG AGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OXa65870
Clone ID Related Accession (Same CDS sequence) XM_012970073.3
Accession Version XM_012970073.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2214bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-17
Organism Xenopus tropicalis(tropical clawed frog)
Product eukaryotic elongation factor 2 kinase isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970073.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCAGACG AAGATCTCAT CTTTAACATG GAGGGCCTTG ATAACATCGA CTTGGCCAAA 
GACCGATGCT CAGATTACAA CAAGGCCGAC AGCGATGAGG AAGACAATTT CTACATCTGC 
CCTATCACCG ACGACCCAGC TGCGCAGCAC CAGACCAAAA AGAAAAGTCC ATGTTTCTTT 
ACCAACTTGG TGAAAAAGAC AGAATATGGA ACTAGTTCCT CCCCTCGGAA CAGTTTTAAG 
GAGGCCTGGA AGCATGCCAT TCAGAAAGCT AAGCAGATGC CTGATCCATG GGCTGAGTTT 
CACTTGGAAG ACATTGAGAC AGAGCATGCT ATCAGGCACA GGTACAATGC TGTATCTGGC 
CAGTGGGTTC AAGATGATGT ACTAATTAAA ATGGCCTCTC AGGCGTTTGG CAGGGGAGCC 
ATGAGAGAAT GTTACCGAAC GAAAAAACTC TCCAACTTCT CACACAAACA GCAGTGGAAG 
AGCGCATATA ATTATGTGTC CAAACGATAC TTAGAGACTG TGGACAGAGA TGTGTACTTT 
GAGGACGTCC GACTCCAGAT GGAGGCAAAG CTGTGGGGAG AGGAATACAA CAGACACAAA 
CCACCAAAAC AGGTGGATAT CATGCAAATG TGCATTATTG AAATGAAGGA CAGGCCTGGC 
AAACCGCTTT TTCACCTTGA ACATTACATC GAGGGAAAGT ATATTAAATA TAACTCAAAT 
TCTGGGTTTG TACGAGATGA CAATATCCGC CTCACTCCTC AGGCCTTCAG TCACTTCACA 
TTTGAGCGCT CAGGACATCA GCTGATTGTT GTTGATGTAC AAGGAGTTGG TGATTTATAC 
ACTGATCCTC AGATCCACAC AGAGAGTGGG ACGGATTTTG GAGATGGAAA TTTAGGGGTA 
CGAGGGATGG CTCTGTTTTT CCATTCTCAT GCTTGCAACA GAATTTGCAA AAGCATGGGA 
CTGACACCCT TTGACATTTC GGCCAAAGAA AAGGCTGTTT TGGATAACTG CAGCAAAATG 
TTGCAAACAG CCCAGACTGT TGTTCGTGGC ATTGAGGAAA AGTGTGGCAG CAATCGGGTC 
AGAACCATGT CTGGCAGCCG CCCACCTCTT CTCTCACGTC TCTCTGAAAA CTCTGGAGAT 
GACATGAGTG ACATGGCGTT CGATTCCTTA CCTAGCTCAC CTTCCCCAGT CACCCCCCAC 
GGCCATCGAT TCGATTCGCA TGGTTGGCCT ATTTTTAATG AGGTCGACAA CTTACTTAAC 
GAAGAAAACA ATAACTTGGA CAATCATAGA GACTCTGAAA ATGGAGGGGA TAGTGGATGT 
CCCAGTGAAA AGAGAAGTGA ATCTGATGAG CTGGAACACA GAATCCATAA CGTCGGCAGG 
AGACGTTTTG AGTCAGAATC CGAAGACAGT TTTGGAAAGA GCAAAGGTGT AAATAAGGCC 
TATGTCATCC TTAAAAACAG TCCAAGGAGT AAACTGGTCA GTGTGGAAAA ATGGAACCAG 
TTCCACGCTT CCCGAGTTCA TGTCCACAGA CCCTCTTGTG TGGCGTTTGA AGTAGAAAGA 
CTTAATGCTC TGGAACTAGA GAAGAAAATC GGGAAATCGA TTCTAGGAAA GGTTCACTTG 
GCCATGGTGC GTTATCATGA AGGGGGGCGT TTCTGCGAGA AAGACCAAGA ATGGGATCAG 
GAATCTGCCA TTTTTCATCT GGAACACGCT GCTCACTGTG GGGAACTTGA AGCCATTATT 
GGCCTTGGCC TCATGTACTC ACAGTCACCC CATCACATAC TGACTGATGT ATCTGTCCAG 
GACACAGAAG ATAATAGGAT AAAAGGATTT AACTATTTGT TGATGGCAGC AGAAGCAGGA 
GACCGTCCTT CCATGATTCA CGTTGCCCGA GCTTTTGATA CTGGTGTGAA CCTTGGTTCA 
GACAGGATAA AGGACTGGCA AGAAGCCCTC TTCTGGTACG ATTCTGCACT CAACATGACA 
GATTATGATG AAGGTGGTGA ATATGATGGA ACCCAAGATG AACCCAAATA CTTGCTGCTG 
GCTAGGGAAG CAGAAATGTT ACTTACTGGG GGATTTAACC TTCAGAAGGA CCCACAGCGA 
TCTGGTGACT TGTACACAGA AGCTGCTGAA GCAGCAATGG AAGCAATGAA AGGGAGGTTG 
GCCAATCAGT ACTACCAGAA GGCAGAGGAG GCCTGGGCGT GTATGGAGGA GTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_012825527.1
CDS305..2518
Translation

Target ORF information:

RefSeq Version XM_012970073.3
Organism Xenopus tropicalis(tropical clawed frog)
Definition Xenopus tropicalis eukaryotic elongation factor 2 kinase (eef2k), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_012970073.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCAGACG AAGATCTCAT CTTTAACATG GAGGGCCTTG ATAACATCGA CTTGGCCAAA 
GACCGATGCT CAGATTACAA CAAGGCCGAC AGCGATGAGG AAGACAATTT CTACATCTGC 
CCTATCACCG ACGACCCAGC TGCGCAGCAC CAGACCAAAA AGAAAAGTCC ATGTTTCTTT 
ACCAACTTGG TGAAAAAGAC AGAATATGGA ACTAGTTCCT CCCCTCGGAA CAGTTTTAAG 
GAGGCCTGGA AGCATGCCAT TCAGAAAGCT AAGCAGATGC CTGATCCATG GGCTGAGTTT 
CACTTGGAAG ACATTGAGAC AGAGCATGCT ATCAGGCACA GGTACAATGC TGTATCTGGC 
CAGTGGGTTC AAGATGATGT ACTAATTAAA ATGGCCTCTC AGGCGTTTGG CAGGGGAGCC 
ATGAGAGAAT GTTACCGAAC GAAAAAACTC TCCAACTTCT CACACAAACA GCAGTGGAAG 
AGCGCATATA ATTATGTGTC CAAACGATAC TTAGAGACTG TGGACAGAGA TGTGTACTTT 
GAGGACGTCC GACTCCAGAT GGAGGCAAAG CTGTGGGGAG AGGAATACAA CAGACACAAA 
CCACCAAAAC AGGTGGATAT CATGCAAATG TGCATTATTG AAATGAAGGA CAGGCCTGGC 
AAACCGCTTT TTCACCTTGA ACATTACATC GAGGGAAAGT ATATTAAATA TAACTCAAAT 
TCTGGGTTTG TACGAGATGA CAATATCCGC CTCACTCCTC AGGCCTTCAG TCACTTCACA 
TTTGAGCGCT CAGGACATCA GCTGATTGTT GTTGATGTAC AAGGAGTTGG TGATTTATAC 
ACTGATCCTC AGATCCACAC AGAGAGTGGG ACGGATTTTG GAGATGGAAA TTTAGGGGTA 
CGAGGGATGG CTCTGTTTTT CCATTCTCAT GCTTGCAACA GAATTTGCAA AAGCATGGGA 
CTGACACCCT TTGACATTTC GGCCAAAGAA AAGGCTGTTT TGGATAACTG CAGCAAAATG 
TTGCAAACAG CCCAGACTGT TGTTCGTGGC ATTGAGGAAA AGTGTGGCAG CAATCGGGTC 
AGAACCATGT CTGGCAGCCG CCCACCTCTT CTCTCACGTC TCTCTGAAAA CTCTGGAGAT 
GACATGAGTG ACATGGCGTT CGATTCCTTA CCTAGCTCAC CTTCCCCAGT CACCCCCCAC 
GGCCATCGAT TCGATTCGCA TGGTTGGCCT ATTTTTAATG AGGTCGACAA CTTACTTAAC 
GAAGAAAACA ATAACTTGGA CAATCATAGA GACTCTGAAA ATGGAGGGGA TAGTGGATGT 
CCCAGTGAAA AGAGAAGTGA ATCTGATGAG CTGGAACACA GAATCCATAA CGTCGGCAGG 
AGACGTTTTG AGTCAGAATC CGAAGACAGT TTTGGAAAGA GCAAAGGTGT AAATAAGGCC 
TATGTCATCC TTAAAAACAG TCCAAGGAGT AAACTGGTCA GTGTGGAAAA ATGGAACCAG 
TTCCACGCTT CCCGAGTTCA TGTCCACAGA CCCTCTTGTG TGGCGTTTGA AGTAGAAAGA 
CTTAATGCTC TGGAACTAGA GAAGAAAATC GGGAAATCGA TTCTAGGAAA GGTTCACTTG 
GCCATGGTGC GTTATCATGA AGGGGGGCGT TTCTGCGAGA AAGACCAAGA ATGGGATCAG 
GAATCTGCCA TTTTTCATCT GGAACACGCT GCTCACTGTG GGGAACTTGA AGCCATTATT 
GGCCTTGGCC TCATGTACTC ACAGTCACCC CATCACATAC TGACTGATGT ATCTGTCCAG 
GACACAGAAG ATAATAGGAT AAAAGGATTT AACTATTTGT TGATGGCAGC AGAAGCAGGA 
GACCGTCCTT CCATGATTCA CGTTGCCCGA GCTTTTGATA CTGGTGTGAA CCTTGGTTCA 
GACAGGATAA AGGACTGGCA AGAAGCCCTC TTCTGGTACG ATTCTGCACT CAACATGACA 
GATTATGATG AAGGTGGTGA ATATGATGGA ACCCAAGATG AACCCAAATA CTTGCTGCTG 
GCTAGGGAAG CAGAAATGTT ACTTACTGGG GGATTTAACC TTCAGAAGGA CCCACAGCGA 
TCTGGTGACT TGTACACAGA AGCTGCTGAA GCAGCAATGG AAGCAATGAA AGGGAGGTTG 
GCCAATCAGT ACTACCAGAA GGCAGAGGAG GCCTGGGCGT GTATGGAGGA GTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OXa65871
Clone ID Related Accession (Same CDS sequence) XM_012970074.3
Accession Version XM_012970074.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2208bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-17
Organism Xenopus tropicalis(tropical clawed frog)
Product eukaryotic elongation factor 2 kinase isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970074.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCAGACG AAGATCTCAT CTTTAACATG GAGGGCCTTG ATAACATCGA CTTGGCCAAA 
GACCGATGCT CAGATTACAA CAAGGCCGAC AGCGATGAGG AAGACAATTT CTACATCTGC 
CCTATCACCG ACGACCCAGC TGCGCAGCAC CAGACCAAAA AGAAAAGTCC ATGTTTCTTT 
ACCAACTTGG TGAAAAAGAC AGAATATGGA ACTAGTTCCT CCCCTCGGAA CAGTTTTAAG 
AATGGACATT CCAAATATTC TAAGCATGGC TGCTTGGCTG AACGAGGTCG GGAGGCCTGG 
AAGCATGCCA TTCAGAAAGC TAAGCAGATG CCTGATCCAT GGGCTGAGTT TCACTTGGAA 
GACATTGAGA CAGAGCATGC TATCAGGCAC AGGTACAATG CTGTATCTGG CCAGTGGGTT 
CAAGATGATG TACTAATTAA AATGGCCTCT CAGGCGTTTG GCAGGGGAGC CATGAGAGAA 
TGTTACCGAA CGAAAAAACT CTCCAACTTC TCACACAAAC AGCAGTGGAA GAGCGCATAT 
AATTATGTGT CCAAACGATA CTTAGAGACT GTGGACAGAG ATGTGTACTT TGAGGACGTC 
CGACTCCAGA TGGAGGCAAA GCTGTGGGGA GAGGAATACA ACAGACACAA ACCACCAAAA 
CAGGTGGATA TCATGCAAAT GTGCATTATT GAAATGAAGG ACAGGCCTGG CAAACCGCTT 
TTTCACCTTG AACATTACAT CGAGGGAAAG TATATTAAAT ATAACTCAAA TTCTGGGTTT 
GTACGAGATG ACAATATCCG CCTCACTCCT CAGGCCTTCA GTCACTTCAC ATTTGAGCGC 
TCAGGACATC AGCTGATTGT TGTTGATGTA CAAGGAGTTG GTGATTTATA CACTGATCCT 
CAGATCCACA CAGAGAGTGG GACGGATTTT GGAGATGGAA ATTTAGGGGT ACGAGGGATG 
GCTCTGTTTT TCCATTCTCA TGCTTGCAAC AGAATTTGCA AAAGCATGGG ACTGACACCC 
TTTGACATTT CGGCCAAAGA AAAGGCTGTT TTGGATAACT GCAGCAAAAT GTTGCAAACA 
GCCCAGACTG TTGTTCGTGG CATTGAGGAA AAGTGTGGCA GCAATCGGGT CAGAACCATG 
TCTGGCAGCC GCCCACCTCT TCTCTCACGT CTCTCTGAAA ACTCTGGAGA TGACATGAGT 
GACATGGCGT TCGATTCCTT ACCTAGCTCA CCTTCCCCAG TCACCCCCCA CGGCCATCGA 
TTCGATTCGC ATGGTTGGCC TATTTTTAAT GAGGTCGACA ACTTACTTAA CGAAGAAAAC 
AATAACTTGG ACAATCATAG AGACTCTGAA AATGGAGGGG ATAGTGGATG TCCCAGTGAA 
AAGAGAAGTG AATCTGATGA GCTGGAACAC AGAATCCATA ACGTCGGCAG GAGACGTTTT 
GAGTCAGAAT CCGAAGACAG TTTTGGAAAG GTCAGTGTGG AAAAATGGAA CCAGTTCCAC 
GCTTCCCGAG TTCATGTCCA CAGACCCTCT TGTGTGGCGT TTGAAGTAGA AAGACTTAAT 
GCTCTGGAAC TAGAGAAGAA AATCGGGAAA TCGATTCTAG GAAAGGTTCA CTTGGCCATG 
GTGCGTTATC ATGAAGGGGG GCGTTTCTGC GAGAAAGACC AAGAATGGGA TCAGGAATCT 
GCCATTTTTC ATCTGGAACA CGCTGCTCAC TGTGGGGAAC TTGAAGCCAT TATTGGCCTT 
GGCCTCATGT ACTCACAGTC ACCCCATCAC ATACTGACTG ATGTATCTGT CCAGGACACA 
GAAGATAATA GGATAAAAGG ATTTAACTAT TTGTTGATGG CAGCAGAAGC AGGAGACCGT 
CCTTCCATGA TTCACGTTGC CCGAGCTTTT GATACTGGTG TGAACCTTGG TTCAGACAGG 
ATAAAGGACT GGCAAGAAGC CCTCTTCTGG TACGATTCTG CACTCAACAT GACAGATTAT 
GATGAAGGTG GTGAATATGA TGGAACCCAA GATGAACCCA AATACTTGCT GCTGGCTAGG 
GAAGCAGAAA TGTTACTTAC TGGGGGATTT AACCTTCAGA AGGACCCACA GCGATCTGGT 
GACTTGTACA CAGAAGCTGC TGAAGCAGCA ATGGAAGCAA TGAAAGGGAG GTTGGCCAAT 
CAGTACTACC AGAAGGCAGA GGAGGCCTGG GCGTGTATGG AGGAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_012825528.1
CDS305..2512
Translation

Target ORF information:

RefSeq Version XM_012970074.3
Organism Xenopus tropicalis(tropical clawed frog)
Definition Xenopus tropicalis eukaryotic elongation factor 2 kinase (eef2k), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_012970074.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCAGACG AAGATCTCAT CTTTAACATG GAGGGCCTTG ATAACATCGA CTTGGCCAAA 
GACCGATGCT CAGATTACAA CAAGGCCGAC AGCGATGAGG AAGACAATTT CTACATCTGC 
CCTATCACCG ACGACCCAGC TGCGCAGCAC CAGACCAAAA AGAAAAGTCC ATGTTTCTTT 
ACCAACTTGG TGAAAAAGAC AGAATATGGA ACTAGTTCCT CCCCTCGGAA CAGTTTTAAG 
AATGGACATT CCAAATATTC TAAGCATGGC TGCTTGGCTG AACGAGGTCG GGAGGCCTGG 
AAGCATGCCA TTCAGAAAGC TAAGCAGATG CCTGATCCAT GGGCTGAGTT TCACTTGGAA 
GACATTGAGA CAGAGCATGC TATCAGGCAC AGGTACAATG CTGTATCTGG CCAGTGGGTT 
CAAGATGATG TACTAATTAA AATGGCCTCT CAGGCGTTTG GCAGGGGAGC CATGAGAGAA 
TGTTACCGAA CGAAAAAACT CTCCAACTTC TCACACAAAC AGCAGTGGAA GAGCGCATAT 
AATTATGTGT CCAAACGATA CTTAGAGACT GTGGACAGAG ATGTGTACTT TGAGGACGTC 
CGACTCCAGA TGGAGGCAAA GCTGTGGGGA GAGGAATACA ACAGACACAA ACCACCAAAA 
CAGGTGGATA TCATGCAAAT GTGCATTATT GAAATGAAGG ACAGGCCTGG CAAACCGCTT 
TTTCACCTTG AACATTACAT CGAGGGAAAG TATATTAAAT ATAACTCAAA TTCTGGGTTT 
GTACGAGATG ACAATATCCG CCTCACTCCT CAGGCCTTCA GTCACTTCAC ATTTGAGCGC 
TCAGGACATC AGCTGATTGT TGTTGATGTA CAAGGAGTTG GTGATTTATA CACTGATCCT 
CAGATCCACA CAGAGAGTGG GACGGATTTT GGAGATGGAA ATTTAGGGGT ACGAGGGATG 
GCTCTGTTTT TCCATTCTCA TGCTTGCAAC AGAATTTGCA AAAGCATGGG ACTGACACCC 
TTTGACATTT CGGCCAAAGA AAAGGCTGTT TTGGATAACT GCAGCAAAAT GTTGCAAACA 
GCCCAGACTG TTGTTCGTGG CATTGAGGAA AAGTGTGGCA GCAATCGGGT CAGAACCATG 
TCTGGCAGCC GCCCACCTCT TCTCTCACGT CTCTCTGAAA ACTCTGGAGA TGACATGAGT 
GACATGGCGT TCGATTCCTT ACCTAGCTCA CCTTCCCCAG TCACCCCCCA CGGCCATCGA 
TTCGATTCGC ATGGTTGGCC TATTTTTAAT GAGGTCGACA ACTTACTTAA CGAAGAAAAC 
AATAACTTGG ACAATCATAG AGACTCTGAA AATGGAGGGG ATAGTGGATG TCCCAGTGAA 
AAGAGAAGTG AATCTGATGA GCTGGAACAC AGAATCCATA ACGTCGGCAG GAGACGTTTT 
GAGTCAGAAT CCGAAGACAG TTTTGGAAAG GTCAGTGTGG AAAAATGGAA CCAGTTCCAC 
GCTTCCCGAG TTCATGTCCA CAGACCCTCT TGTGTGGCGT TTGAAGTAGA AAGACTTAAT 
GCTCTGGAAC TAGAGAAGAA AATCGGGAAA TCGATTCTAG GAAAGGTTCA CTTGGCCATG 
GTGCGTTATC ATGAAGGGGG GCGTTTCTGC GAGAAAGACC AAGAATGGGA TCAGGAATCT 
GCCATTTTTC ATCTGGAACA CGCTGCTCAC TGTGGGGAAC TTGAAGCCAT TATTGGCCTT 
GGCCTCATGT ACTCACAGTC ACCCCATCAC ATACTGACTG ATGTATCTGT CCAGGACACA 
GAAGATAATA GGATAAAAGG ATTTAACTAT TTGTTGATGG CAGCAGAAGC AGGAGACCGT 
CCTTCCATGA TTCACGTTGC CCGAGCTTTT GATACTGGTG TGAACCTTGG TTCAGACAGG 
ATAAAGGACT GGCAAGAAGC CCTCTTCTGG TACGATTCTG CACTCAACAT GACAGATTAT 
GATGAAGGTG GTGAATATGA TGGAACCCAA GATGAACCCA AATACTTGCT GCTGGCTAGG 
GAAGCAGAAA TGTTACTTAC TGGGGGATTT AACCTTCAGA AGGACCCACA GCGATCTGGT 
GACTTGTACA CAGAAGCTGC TGAAGCAGCA ATGGAAGCAA TGAAAGGGAG GTTGGCCAAT 
CAGTACTACC AGAAGGCAGA GGAGGCCTGG GCGTGTATGG AGGAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P