ORF cDNA Clones and Custom Clones » Species Summary » Xenopus tropicalis » chd8 cDNA ORF clone

chd8 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

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Summary

Gene Symbol	chd8
Entrez Gene ID	100192376
Full Name	chromodomain helicase DNA binding protein 8
Synonyms	CHD-8,duplin
General protein information	Preferred Names chromodomain helicase DNA binding protein 8 Names chromodomain-helicase-DNA-binding protein 8 ATP-dependent helicase CHD8
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012957172.3	XP_012812626.1	chromodomain-helicase-DNA-binding protein 8 isoform X1
NM_001137617.2	NP_001131089.2	chromodomain-helicase-DNA-binding protein 8
XM_012957178.3	XP_012812632.1	chromodomain-helicase-DNA-binding protein 8 isoform X1
XM_031896082.1	XP_031751942.1	chromodomain-helicase-DNA-binding protein 8 isoform X1
XM_012957198.3	XP_012812652.1	chromodomain-helicase-DNA-binding protein 8 isoform X2
XM_012957178.2	XP_012812632.1	chromodomain-helicase-DNA-binding protein 8 isoform X1
XM_012957198.2	XP_012812652.1	chromodomain-helicase-DNA-binding protein 8 isoform X2
XM_012957172.2	XP_012812626.1	chromodomain-helicase-DNA-binding protein 8 isoform X1
XM_012957182.2	XP_012812636.1	chromodomain-helicase-DNA-binding protein 8 isoform X1
XM_012957182.3	XP_012812636.1	chromodomain-helicase-DNA-binding protein 8 isoform X1

Homologies

	CHD8	XP_001096619.2
Bos taurus (cattle)	CHD8	NP_001179063.1
Danio rerio (zebrafish)	chd8	NP_001189381.1
Canis lupus familiaris (dog)	CHD8	XP_532624.4
Rattus norvegicus (Norway rat)	Chd8	NP_075222.2
Pan troglodytes (chimpanzee)	CHD8	XP_003314292.1
Mus musculus (house mouse)	Chd8	NP_963999.2
Homo sapiens (human)	CHD8	NP_001164100.1
Xenopus tropicalis (tropical clawed frog)	chd8	NP_001131089.2

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

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The following chd8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the chd8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa32500	XM_031896082.1 Latest version!	Xenopus tropicalis chromodomain helicase DNA binding protein 8 (chd8), transcript variant X4, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	29-31	$1301.30 $1859.00
OXa32500	XM_012957182.3 Latest version!	Xenopus tropicalis chromodomain helicase DNA binding protein 8 (chd8), transcript variant X3, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	29-31	$1301.30 $1859.00
OXa32500	XM_012957172.3 Latest version!	Xenopus tropicalis chromodomain helicase DNA binding protein 8 (chd8), transcript variant X1, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	29-31	$1301.30 $1859.00
OXa32500	XM_012957178.3 Latest version!	Xenopus tropicalis chromodomain helicase DNA binding protein 8 (chd8), transcript variant X2, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	29-31	$1301.30 $1859.00
OXa32501	XM_012957198.3 Latest version!	Xenopus tropicalis chromodomain helicase DNA binding protein 8 (chd8), transcript variant X5, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	29-31	$1301.30 $1859.00
OXa01041	NM_001137617.2 Latest version!	Xenopus tropicalis chromodomain helicase DNA binding protein 8 (chd8), mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	30	$1161.30 $1659.00
View Old Accession Versions >>
OXa32500	XM_012957178.2	Xenopus tropicalis chromodomain helicase DNA binding protein 8 (chd8), transcript variant X2, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	30	$1371.30 $1959.00
OXa32501	XM_012957198.2	Xenopus tropicalis chromodomain helicase DNA binding protein 8 (chd8), transcript variant X4, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	30	$1371.30 $1959.00
OXa32500	XM_012957172.2	Xenopus tropicalis chromodomain helicase DNA binding protein 8 (chd8), transcript variant X1, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	30	$1371.30 $1959.00
OXa32500	XM_012957182.2	Xenopus tropicalis chromodomain helicase DNA binding protein 8 (chd8), transcript variant X3, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	30	$1371.30 $1959.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

chd8 ( XM_012957172.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012812626.1 Xenopus tropicalis chromodomain helicase DNA binding protein 8 (chd8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa32500
Clone ID Related Accession (Same CDS sequence)	XM_012957172.3 , XM_012957178.3 , XM_031896082.1 , XM_012957178.2 , XM_012957172.2 , XM_012957182.2 , XM_012957182.3
Accession Version	XM_012957172.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7008bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	chromodomain-helicase-DNA-binding protein 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957172.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACC CTATCATGGA CCTCTTTGAT GATCCAAACC TATTTGGCCT TGACTCTCTC 
ACTGGAGATT CCTTTGGACG GGATGGGCCA GACACTATAG ATGATGCTCT AGGCCTGGGT 
AATGTCTTGG GACCTTTGGA GCCGATAACA GACAGAGTTG GCCTGCCTGG TGCTGATGTT 
GGCAATAATG AAGTTAAACA ACAAACAGAG TCACAGGTTC TCCCGGACAA TCCAGCTCTT 
ATGTCAGCTA CTGAGATCAT GCAGCCCATT CAACTTCCCA CAAACCAGGA AACACTGAAC 
CAAGGAAATC CCTTTATGGG TGCCTCAAAT GCTGGAGCCC CAAAAATTAT GATTCTGAAA 
GCTCCTGCTG GAATGACAGT TGGTGCATGT CCTGTAACTC AGATACAAAC TCTGACACCT 
CACCAAGCTG CTAATGGTGG CAAGGTGGCA TTTGCTAAGG TGCTTACAGG AACCCCATTG 
CGTCCTGGGG TCTCAATTGT ATCTGGAAAC ACTGTCCTTG CCAAAATGCC AAGCACTGGA 
GTTGCAGGGC AGGTAGGAGC AGTGAGGCCT GTCCGTCAGC TACTTCTTCA ACCAGTGCGT 
GCCTCCGCAG CCCCAGGAAG CTCCGAGAGT AATACAGGGA TCAAGCCTGC AATCACACTA 
ACGTCAGCAC CTCAACAGGG GGACCCTAAA AGAATTACTC TCGTCCTACA GCAGCCTTCC 
CAAGTTGGTG CAACTGCACA ACAAGGGCAG CGTCATGTTG TGCTGGGTGG GCTTCCAGGA 
AAGATTGTCC TTCAGGGAAA CCAACTGGCA GCTCTCACAC AAGCTAAAAC CCCTCAAGGT 
CAGCCAGCTA AAGTAGTGAC TATCCAGCTG CAAGTCCAGC AGCAGCCTGG GGCTGCAGGT 
CAGACTCCTC AAAAGTTTCA GATTGTACAA CAAGCCCCAG GAGGTGTGGC AATTGCTCCC 
AGTGGGCAAC ATACACACAT GCTTAATCAA CAAGGAGTTC AGAGGCTTTC TGTTCCACTC 
AAAGTTGTTC TGCAACCACA GGCTGGTTCC TCTCAGGGTA CTGCGCAGGG CCTGTCAGTT 
GTGAAAGTTC TAAATGCAAG TGAAGTTGCC AATTTGACTG CAAGTCAGAC TGTGGTTAAA 
ACATCCACTG GAGGTGGTGA ATCTCGTAAG CTGGACAGCC AGAAGAAGCA AGAGAAAGCT 
AACCGTATTG TAGCTGAAGC TATTGCCAGG GCCCGAGCCA GAGGTGAACA GAATATTCCT 
CGGGTCCTGA ATGAGGATGA GCTTCCCAGT GTGAATCCTG AAGATGATGA TGGAAGCAGG 
CGGAAAAGGA AGAAGAAGAA AGGAGAGACT AGTGATAGGT CCAAGGATGA GAAACCTAAG 
AAGGTCAAAG GTAGTGGAAG CTTAAGGTCT AGGAGCAAAG GCAAACCAAG TACTATCACT 
CCTATTGTGG GTAAGAAAAG GAAGAGAAAC CCATCTTCCG ATAATTCTGA TGCTGAGATC 
ATGGCATCAC AGGCTTCTCC TGCAGAGGAT GAAGACAGTG TCCAGAAGAG ACGTTCAAAT 
CGCCAGGTGA AGCGCAAGAA GTATACAGAG GATTTGGATA TTAAAATAAC AGATGATGAA 
GATGATGAGG AGGTAGATGT GACGGGACCT GTGAAAACAG AACCTGTGCT GCTTCCTGAG 
CCCATGCCTT TACCTGACAG TGAGGCAGTC CCTTCAATGC AGTTCTTCGT GGAGAACCCA 
AGTGAAGAAG ATGCTGCTAT TGTTGACAAA ATACTCTCTA TGCGCCTAGC TAAAAAAGAG 
CTGCCCACTG GAGAGTATGT TGAAGTTGAA GAGTACTTTG TCAAATATAA AAATTATTCC 
TACCTACACT GTGAATGGGC CACAATTGAG CAACTGGAAA GGGACAAGCG TATTCATCAG 
AAACTGAAAA GATTTAAGAC AAAGATGACC CAGATGCAGC ATTTCTTGCA AGAAGATGAA 
GAATCTTTCA ATCCAGACTA TGTAGAGGTA GATCGAATCC TAGACGAATC TCACAGCACT 
GACAAGGACA ATGGTGAACC GGTGGTGTAT TACCTAGTAA AATGGTGCTC TTTACCTTAT 
GAAGATAGCA CATGGGAGCT GAAAGAAGAT GTTGATGATG GAAAGATAGA GGAATTCAAA 
CGAATAGAGG CGAGGCAGCC AAATCTCAAA CGCGTGGCCC GCCCAGCTGC TACATCATGG 
AAGAAGCTGG AATTGTCTCG GGAGTATCAG AATGGCAACC AGTTGAGAGA ATACCAGCTG 
GAAGGTGTTA ATTGGCTTCT CTTTAACTGG TACAATAGGC AAAACTGCAT TTTGGCAGAT 
GAAATGGGTT TGGGGAAGAC CATCCAATCT ATCACATTCT TACAAGAAGT ATATAATGTG 
GGAATACGGG GACCTTTTTT AGTAATTGCT CCACTTTCCA CCATCACGAA CTGGGAGAGA 
GAGTTTGGCA GTTGGACTCA AATGAATACT ATTGTGTATC ATGGCAGCCT TGCTAGTCGC 
CAAATGATCC AGCAATATGA GATGTACTGT AAGGACTCCA AGGGTCGTCT TATTCCTGGT 
GCGTACAAGT TTGATGCTCT TATTACAACT TTTGAAATGG TGCTGTCTGA TTGCCCAGAG 
CTGCGTGAAA TTGAGTGGCG CTGTGTCATA ATTGATGAGG CTCATCGGCT CAAAAACAGA 
AACTGCAAAC TACTGGACAG CCTAAAGCAC ATGGACTTGG AGCACAAAGT CCTTTTAACA 
GGCACTCCAT TACAGAACAC CGTGGAAGAG TTGTTCAGTC TTTTGCATTT CCTGGAGCCA 
ACACAATTTT CCTCTGAAGC AGAGTTTCTT AAGGACTTTG GAGACCTGAA GACAGAGGAA 
CAGGTGCAGA AATTACAGGC CATCTTAAAA CCTATGATGT TGAGGCGATT AAAGGAAGAT 
GTTGAGAAAA ACCTGGCCCC GAAACAAGAA ACTATAATTG AGGTTGAGCT TACTAACATC 
CAGAAGAAGT ATTACCGAGC AATACTGGAG AAGAATTTTT CTTTTCTGAC CAAAGGAGCT 
AGTCAATCCA ACACACCCAA CTTGCTCAAT ACCATGATGG AACTACGCAA ATGCTGCAAC 
CACCCATACC TCATCACTGG AGCAGAAGAA AAGATCATAT CTGAGTTCCG AGAAGCAACT 
CCAGTAGTTC CCCCTGACTT TCACGTCCAA GCAATGGTAC GCTCCTCTGG CAAACTGGTG 
CTAATAGATA AGCTGCTCCC TAAACTAAGG GCTGGAGGCC ACAAAGTTCT CATCTTTTCT 
CAGATGGTAC GGTGCCTGGA CATCCTGGAA GACTATCTCA TTCAGAGAAG GTACCTTTAT 
GAGCGTATTG ATGGACGTGT CCGTGGCAAC ATGCGTCAGG CAGCCATTGA TAGATTCAGC 
CGCCCAGATT CTGACCGATT TGTGTTTCTA CTCTGTACAC GAGCTGGTGG GCTTGGAATT 
AACTTGACAG CTGCTGATAC TTGTATTATA TTTGACTCGG ACTGGAACCC TCAAAATGAC 
CTTCAGGCTC AAGCTCGATG TCATCGTATT GGGCAAAGCA AAGCCGTTAA AATTTACAGA 
CTCATCACTC GCAACTCCTA TGAGAGAGAG ATGTTTGATA AGGCCAGTCT CAAACTAGGA 
CTAGACAAAG CTGTATTGCA GTCTATGAGT GGTCGGGATA ATCACCTTAG TGGGCCTATT 
CAGCAATTCA CAAAGAAAGA AATTGAAGAC TTGCTAAGGA AAGGAGCTTA TGCTGCAATC 
ATGGATGAAG ATGACGAAGG CTCTAAATTC TGTGAAGAAG ATATTGACCA AATTTTACTG 
AGGCGTACCA CGACTATTAC CATAGAGTCA GAGGGAAAGG GCTCAACCTT CTCCAAGGCA 
AGTTTTGTTG CTTCTGAGAA CAGGACTGAT ATATCCCTAG ATGATCCTAA TTTCTGGCAA 
AAGTGGGCCA AGAAAGCAGA CTTAGACTTG GATCTTCTTA GCAGCAAGAA TACACTGGTT 
ATTGATACCC CACGTATCAG GAAGCAAACA CGGCACTTTA CTAACAAAGA TGATGACATG 
GTTGAGTTCT CAGATTTGGA GAGTGATGAT GATGATCGCC CCAAAGCTCG GCGTCAAGAC 
CGCAAGCATG GATATGGACG TACAGATTGC TTCCGTGTGG AGAAGCATTT GCTTGTTTAT 
GGATGGGGTC GCTGGCGAGA TATCCTTACT CATGGTCGTT TCAAGCGTGG TATGAATGAG 
CGTGACGTGG AGAAAATATG TAGAGCCATA CTGGTTTATT GTCTTTTGCA CTACCGGGGA 
GATGAAAACA TAAAGAGCTT TATCTGGGAT CTAATTACAC CAGCTGAGAA TGGCAAAACT 
AAGCAGCTAC AGAACCACTC TGGATTGTCT ATTCCTGTCC CCCGTGGTCG TAAGGGGAAA 
AAAGTGAAAT CCCAAAGCAG CTTTGACATA CATAAAGCCG ATTGGATACA TAAGTACAAC 
CCAGATACCT TGTTCCAAGA TGAAGGGTAC AAGAAACATC TCAAGCATCA ATGTAACAAG 
GTGCTGCTTC GCGTTCGTAT GTTATATTTT CTTCGTCAGG AAGTTATTGG AAACCAGGCA 
ATGAAAGTTT TAAGTGGTGT AGAGGCCAGG CAAATTGACA TCTGGTTCCC ACAGGTGGAC 
CAGGTTGAAG TTCCCTCACG CTGGTGGGAC AGAGAAGCTG ATAAATCTCT TCTTATCGGG 
GTCTTTAAAC ATGGTTATGA GAAGTATAAC ACCATGCGAG CTGACCCTGC ACTCTGTTTT 
TTGGAGAAGG TGGGGGGACC AGATGAACAG GCTATTGCAG CTGAGCACCA TGCTCAAGAC 
TTTTCTGAAC TCCCTGATGG TGGGGACTTT GATCGAGACA TAGAAGATCC AGAATACAAG 
CCACTTCATG CACAGAAAGA TCCTGAGGAT GAGATCGACT CTTTAATGAT GGATGAAGAA 
ATCTCTGTTG TTGACGGTGA AGAAGCAACA GCAATGCCCA GTGGTATGTT ATGGCCACCT 
GGCTCTGCTC TCACAGCTCG TCTACGGCGT CTAATTACTG CCTACCAACG CAGCTACAAA 
AGAGAACAAC TAAAATTGGA GGCTGAAGAG CGTGGGGACA AACGTAGAAA ACGATGTGAG 
GCAGCCTTTA AACTGAAAGA AATTGCCCGT AGGGAGAAAC AGCAAAGGTG GACCCGAAGG 
GAGGCATGTG ACTTTTTTCG TGTGCTGTCC AGCTTTGGTG TCGAGTATGA CCCCGATACT 
CAACTGTATG ACTGGCAGAG GTTCCGTGGT CTGGCAAGAC TGGACAAGAA GACAGATGAG 
TCACTGCTCA AATACTTCCA TGGATTTGTG GCAATGTGCC GCCAGGTTTG CCGCTTACCC 
CCTGCTGCCG GAGACGAGCC ACCAGATCCA TCTCTCTTCA TTGAGCCAAT TTCAGAAGAG 
CGAGCATCTC GCGCCCTGTT TCGCTTGGAT CTTCTTCGTA GGGTGCGTGA GCAAGTACTT 
TGTCATCCTT TGCTTATTCC TCGTTTGTCA CTGTGCCGGC CAGACCCAGA ACTCCCAGAA 
TGGTGGGAGA GTGGACGTCA TGACAATGAG CTTCTTCAGG GAGCAGCACG TTATGGTCTG 
AGCCGTACAG ACCTCACTAT ACTGCAGGAC CATGCATTTT CTTTTTTGCG GTGTCAGATC 
AGTTATTTCC AGTCAAGGGG AATATCTGGC CATTCTCGCC CTTCTACTCC AACTACTGCC 
CCTTCAGTGC CTACTGAACG TCAGCCTTCT CATCTAGCCT CCCTTTCCTC ATCTGTCAGC 
TGTTCTCCTG CTCCACGCAG ATCCTCTTCC TGCTCATCAT CATCTCACTC ATCAAACTCT 
GAGGATTCTT CAGATGAAAG CAATGGTGTA AAAGTAAAAC CTAATGCAGG TCTTTCGCAC 
GCTCGACTGT ATGATGAGGA GAGCCGGCTT TCTCTTACTG CCTCCCCTGC AGATCTCACT 
ACAGAGGACA GTATTCAGAC AGCGCTGGAG ACCCCGCACA GTACAGACTG GCCAAAGGAC 
CGTGTCCTTA TCTGCAGAAT TGAGCAAGTA TGCAGTGCCG TTCTTACTGG CAAATGGTCC 
TCACCACGAC GACTGTCAGA TCCACCAAGT GACAGCCCTG ATAGCTTGCC TCCAACCCCA 
GAGCAGCAGA GTCCAGCCCA CTTCACACAG ATAAGACCTG CACCGGACCC CAAAGAGTTT 
ACCATTCAAA TAAAAAGTGA AGAAGGCCTG AAGATTAAAT TTCAGAAGCA CAAATTTATG 
GGTAATGGAG CTCTTGATTC TGCACCTCAT ACCCAGAAGA AAAAGAGCAA GAAAAAGATG 
GATCTTGACA TGGACACACG AATTCCCGTT GTAAACCAGA AGGATGGGAC CTTGTTGTTA 
GGGGAAGAAG CCCCTTTACG CTGCCAGCTG CCCGAGTGGT TACACAGAAA TCCTGACTTC 
ATGGTTGACC CACGCTTTAT TGCGTTTATG GAAGACCGCA GGAAAAGAAA AAAACATCGA 
AGCAAAAAGT CCAAATCCTC CTCCCAGATT GGAAACGTAC AGAATCCCCA CTCCCACAAT 
GGAAAGAAGT CTTCCCACAC AGAAAACTCT TTCGAGACGC AACACCTTCC TACTGCAGCC 
AAAAAAAGGA AGAAACGCCC TCCTTCAAAA ATAATAATGC CATCTGCACT ACATGGAGCT 
GGTGATGTTA CACATTCCTT CTCAGACCTT CAACCTGACC ATTCTGGTCA CCCCATGCGG 
TCAGTGTATG GACCATCAGA TGGAGGGCTG TCCCATCAAG CAGGACTCAG CGGGTTGGGA 
TTTACCCGCT TTCATTCATC GCAGACATCT TCAGTGGGCA GAATCCATGG CACTACCTTT 
GATGAGTTAC AAAGTTTTGA GCCCCCACAA CAACATTTTT CTCTGCTTGA TGATCCTATG 
ATGCCAGCAA ATTCCTCCTC CAGCGGTGAT GAACTGGACA GACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012812626.1
CDS	289..7296
Translation	MADPIMDLFDDPNLFGLDSLTGDSFGRDGPDTIDDALGLGNVLGPLEPITDRVGLPGADVGNNEVKQQTESQVLPDNPALMSATEIMQPIQLPTNQETLNQGNPFMGASNAGAPKIMILKAPAGMTVGACPVTQIQTLTPHQAANGGKVAFAKVLTGTPLRPGVSIVSGNTVLAKMPSTGVAGQVGAVRPVRQLLLQPVRASAAPGSSESNTGIKPAITLTSAPQQGDPKRITLVLQQPSQVGATAQQGQRHVVLGGLPGKIVLQGNQLAALTQAKTPQGQPAKVVTIQLQVQQQPGAAGQTPQKFQIVQQAPGGVAIAPSGQHTHMLNQQGVQRLSVPLKVVLQPQAGSSQGTAQGLSVVKVLNASEVANLTASQTVVKTSTGGGESRKLDSQKKQEKANRIVAEAIARARARGEQNIPRVLNEDELPSVNPEDDDGSRRKRKKKKGETSDRSKDEKPKKVKGSGSLRSRSKGKPSTITPIVGKKRKRNPSSDNSDAEIMASQASPAEDEDSVQKRRSNRQVKRKKYTEDLDIKITDDEDDEEVDVTGPVKTEPVLLPEPMPLPDSEAVPSMQFFVENPSEEDAAIVDKILSMRLAKKELPTGEYVEVEEYFVKYKNYSYLHCEWATIEQLERDKRIHQKLKRFKTKMTQMQHFLQEDEESFNPDYVEVDRILDESHSTDKDNGEPVVYYLVKWCSLPYEDSTWELKEDVDDGKIEEFKRIEARQPNLKRVARPAATSWKKLELSREYQNGNQLREYQLEGVNWLLFNWYNRQNCILADEMGLGKTIQSITFLQEVYNVGIRGPFLVIAPLSTITNWEREFGSWTQMNTIVYHGSLASRQMIQQYEMYCKDSKGRLIPGAYKFDALITTFEMVLSDCPELREIEWRCVIIDEAHRLKNRNCKLLDSLKHMDLEHKVLLTGTPLQNTVEELFSLLHFLEPTQFSSEAEFLKDFGDLKTEEQVQKLQAILKPMMLRRLKEDVEKNLAPKQETIIEVELTNIQKKYYRAILEKNFSFLTKGASQSNTPNLLNTMMELRKCCNHPYLITGAEEKIISEFREATPVVPPDFHVQAMVRSSGKLVLIDKLLPKLRAGGHKVLIFSQMVRCLDILEDYLIQRRYLYERIDGRVRGNMRQAAIDRFSRPDSDRFVFLLCTRAGGLGINLTAADTCIIFDSDWNPQNDLQAQARCHRIGQSKAVKIYRLITRNSYEREMFDKASLKLGLDKAVLQSMSGRDNHLSGPIQQFTKKEIEDLLRKGAYAAIMDEDDEGSKFCEEDIDQILLRRTTTITIESEGKGSTFSKASFVASENRTDISLDDPNFWQKWAKKADLDLDLLSSKNTLVIDTPRIRKQTRHFTNKDDDMVEFSDLESDDDDRPKARRQDRKHGYGRTDCFRVEKHLLVYGWGRWRDILTHGRFKRGMNERDVEKICRAILVYCLLHYRGDENIKSFIWDLITPAENGKTKQLQNHSGLSIPVPRGRKGKKVKSQSSFDIHKADWIHKYNPDTLFQDEGYKKHLKHQCNKVLLRVRMLYFLRQEVIGNQAMKVLSGVEARQIDIWFPQVDQVEVPSRWWDREADKSLLIGVFKHGYEKYNTMRADPALCFLEKVGGPDEQAIAAEHHAQDFSELPDGGDFDRDIEDPEYKPLHAQKDPEDEIDSLMMDEEISVVDGEEATAMPSGMLWPPGSALTARLRRLITAYQRSYKREQLKLEAEERGDKRRKRCEAAFKLKEIARREKQQRWTRREACDFFRVLSSFGVEYDPDTQLYDWQRFRGLARLDKKTDESLLKYFHGFVAMCRQVCRLPPAAGDEPPDPSLFIEPISEERASRALFRLDLLRRVREQVLCHPLLIPRLSLCRPDPELPEWWESGRHDNELLQGAARYGLSRTDLTILQDHAFSFLRCQISYFQSRGISGHSRPSTPTTAPSVPTERQPSHLASLSSSVSCSPAPRRSSSCSSSSHSSNSEDSSDESNGVKVKPNAGLSHARLYDEESRLSLTASPADLTTEDSIQTALETPHSTDWPKDRVLICRIEQVCSAVLTGKWSSPRRLSDPPSDSPDSLPPTPEQQSPAHFTQIRPAPDPKEFTIQIKSEEGLKIKFQKHKFMGNGALDSAPHTQKKKSKKKMDLDMDTRIPVVNQKDGTLLLGEEAPLRCQLPEWLHRNPDFMVDPRFIAFMEDRRKRKKHRSKKSKSSSQIGNVQNPHSHNGKKSSHTENSFETQHLPTAAKKRKKRPPSKIIMPSALHGAGDVTHSFSDLQPDHSGHPMRSVYGPSDGGLSHQAGLSGLGFTRFHSSQTSSVGRIHGTTFDELQSFEPPQQHFSLLDDPMMPANSSSSGDELDRP

Target ORF information:

RefSeq Version	XM_012957172.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis chromodomain helicase DNA binding protein 8 (chd8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957172.3

ORF Insert Sequence:

ATGGCTGACC CTATCATGGA CCTCTTTGAT GATCCAAACC TATTTGGCCT TGACTCTCTC 
ACTGGAGATT CCTTTGGACG GGATGGGCCA GACACTATAG ATGATGCTCT AGGCCTGGGT 
AATGTCTTGG GACCTTTGGA GCCGATAACA GACAGAGTTG GCCTGCCTGG TGCTGATGTT 
GGCAATAATG AAGTTAAACA ACAAACAGAG TCACAGGTTC TCCCGGACAA TCCAGCTCTT 
ATGTCAGCTA CTGAGATCAT GCAGCCCATT CAACTTCCCA CAAACCAGGA AACACTGAAC 
CAAGGAAATC CCTTTATGGG TGCCTCAAAT GCTGGAGCCC CAAAAATTAT GATTCTGAAA 
GCTCCTGCTG GAATGACAGT TGGTGCATGT CCTGTAACTC AGATACAAAC TCTGACACCT 
CACCAAGCTG CTAATGGTGG CAAGGTGGCA TTTGCTAAGG TGCTTACAGG AACCCCATTG 
CGTCCTGGGG TCTCAATTGT ATCTGGAAAC ACTGTCCTTG CCAAAATGCC AAGCACTGGA 
GTTGCAGGGC AGGTAGGAGC AGTGAGGCCT GTCCGTCAGC TACTTCTTCA ACCAGTGCGT 
GCCTCCGCAG CCCCAGGAAG CTCCGAGAGT AATACAGGGA TCAAGCCTGC AATCACACTA 
ACGTCAGCAC CTCAACAGGG GGACCCTAAA AGAATTACTC TCGTCCTACA GCAGCCTTCC 
CAAGTTGGTG CAACTGCACA ACAAGGGCAG CGTCATGTTG TGCTGGGTGG GCTTCCAGGA 
AAGATTGTCC TTCAGGGAAA CCAACTGGCA GCTCTCACAC AAGCTAAAAC CCCTCAAGGT 
CAGCCAGCTA AAGTAGTGAC TATCCAGCTG CAAGTCCAGC AGCAGCCTGG GGCTGCAGGT 
CAGACTCCTC AAAAGTTTCA GATTGTACAA CAAGCCCCAG GAGGTGTGGC AATTGCTCCC 
AGTGGGCAAC ATACACACAT GCTTAATCAA CAAGGAGTTC AGAGGCTTTC TGTTCCACTC 
AAAGTTGTTC TGCAACCACA GGCTGGTTCC TCTCAGGGTA CTGCGCAGGG CCTGTCAGTT 
GTGAAAGTTC TAAATGCAAG TGAAGTTGCC AATTTGACTG CAAGTCAGAC TGTGGTTAAA 
ACATCCACTG GAGGTGGTGA ATCTCGTAAG CTGGACAGCC AGAAGAAGCA AGAGAAAGCT 
AACCGTATTG TAGCTGAAGC TATTGCCAGG GCCCGAGCCA GAGGTGAACA GAATATTCCT 
CGGGTCCTGA ATGAGGATGA GCTTCCCAGT GTGAATCCTG AAGATGATGA TGGAAGCAGG 
CGGAAAAGGA AGAAGAAGAA AGGAGAGACT AGTGATAGGT CCAAGGATGA GAAACCTAAG 
AAGGTCAAAG GTAGTGGAAG CTTAAGGTCT AGGAGCAAAG GCAAACCAAG TACTATCACT 
CCTATTGTGG GTAAGAAAAG GAAGAGAAAC CCATCTTCCG ATAATTCTGA TGCTGAGATC 
ATGGCATCAC AGGCTTCTCC TGCAGAGGAT GAAGACAGTG TCCAGAAGAG ACGTTCAAAT 
CGCCAGGTGA AGCGCAAGAA GTATACAGAG GATTTGGATA TTAAAATAAC AGATGATGAA 
GATGATGAGG AGGTAGATGT GACGGGACCT GTGAAAACAG AACCTGTGCT GCTTCCTGAG 
CCCATGCCTT TACCTGACAG TGAGGCAGTC CCTTCAATGC AGTTCTTCGT GGAGAACCCA 
AGTGAAGAAG ATGCTGCTAT TGTTGACAAA ATACTCTCTA TGCGCCTAGC TAAAAAAGAG 
CTGCCCACTG GAGAGTATGT TGAAGTTGAA GAGTACTTTG TCAAATATAA AAATTATTCC 
TACCTACACT GTGAATGGGC CACAATTGAG CAACTGGAAA GGGACAAGCG TATTCATCAG 
AAACTGAAAA GATTTAAGAC AAAGATGACC CAGATGCAGC ATTTCTTGCA AGAAGATGAA 
GAATCTTTCA ATCCAGACTA TGTAGAGGTA GATCGAATCC TAGACGAATC TCACAGCACT 
GACAAGGACA ATGGTGAACC GGTGGTGTAT TACCTAGTAA AATGGTGCTC TTTACCTTAT 
GAAGATAGCA CATGGGAGCT GAAAGAAGAT GTTGATGATG GAAAGATAGA GGAATTCAAA 
CGAATAGAGG CGAGGCAGCC AAATCTCAAA CGCGTGGCCC GCCCAGCTGC TACATCATGG 
AAGAAGCTGG AATTGTCTCG GGAGTATCAG AATGGCAACC AGTTGAGAGA ATACCAGCTG 
GAAGGTGTTA ATTGGCTTCT CTTTAACTGG TACAATAGGC AAAACTGCAT TTTGGCAGAT 
GAAATGGGTT TGGGGAAGAC CATCCAATCT ATCACATTCT TACAAGAAGT ATATAATGTG 
GGAATACGGG GACCTTTTTT AGTAATTGCT CCACTTTCCA CCATCACGAA CTGGGAGAGA 
GAGTTTGGCA GTTGGACTCA AATGAATACT ATTGTGTATC ATGGCAGCCT TGCTAGTCGC 
CAAATGATCC AGCAATATGA GATGTACTGT AAGGACTCCA AGGGTCGTCT TATTCCTGGT 
GCGTACAAGT TTGATGCTCT TATTACAACT TTTGAAATGG TGCTGTCTGA TTGCCCAGAG 
CTGCGTGAAA TTGAGTGGCG CTGTGTCATA ATTGATGAGG CTCATCGGCT CAAAAACAGA 
AACTGCAAAC TACTGGACAG CCTAAAGCAC ATGGACTTGG AGCACAAAGT CCTTTTAACA 
GGCACTCCAT TACAGAACAC CGTGGAAGAG TTGTTCAGTC TTTTGCATTT CCTGGAGCCA 
ACACAATTTT CCTCTGAAGC AGAGTTTCTT AAGGACTTTG GAGACCTGAA GACAGAGGAA 
CAGGTGCAGA AATTACAGGC CATCTTAAAA CCTATGATGT TGAGGCGATT AAAGGAAGAT 
GTTGAGAAAA ACCTGGCCCC GAAACAAGAA ACTATAATTG AGGTTGAGCT TACTAACATC 
CAGAAGAAGT ATTACCGAGC AATACTGGAG AAGAATTTTT CTTTTCTGAC CAAAGGAGCT 
AGTCAATCCA ACACACCCAA CTTGCTCAAT ACCATGATGG AACTACGCAA ATGCTGCAAC 
CACCCATACC TCATCACTGG AGCAGAAGAA AAGATCATAT CTGAGTTCCG AGAAGCAACT 
CCAGTAGTTC CCCCTGACTT TCACGTCCAA GCAATGGTAC GCTCCTCTGG CAAACTGGTG 
CTAATAGATA AGCTGCTCCC TAAACTAAGG GCTGGAGGCC ACAAAGTTCT CATCTTTTCT 
CAGATGGTAC GGTGCCTGGA CATCCTGGAA GACTATCTCA TTCAGAGAAG GTACCTTTAT 
GAGCGTATTG ATGGACGTGT CCGTGGCAAC ATGCGTCAGG CAGCCATTGA TAGATTCAGC 
CGCCCAGATT CTGACCGATT TGTGTTTCTA CTCTGTACAC GAGCTGGTGG GCTTGGAATT 
AACTTGACAG CTGCTGATAC TTGTATTATA TTTGACTCGG ACTGGAACCC TCAAAATGAC 
CTTCAGGCTC AAGCTCGATG TCATCGTATT GGGCAAAGCA AAGCCGTTAA AATTTACAGA 
CTCATCACTC GCAACTCCTA TGAGAGAGAG ATGTTTGATA AGGCCAGTCT CAAACTAGGA 
CTAGACAAAG CTGTATTGCA GTCTATGAGT GGTCGGGATA ATCACCTTAG TGGGCCTATT 
CAGCAATTCA CAAAGAAAGA AATTGAAGAC TTGCTAAGGA AAGGAGCTTA TGCTGCAATC 
ATGGATGAAG ATGACGAAGG CTCTAAATTC TGTGAAGAAG ATATTGACCA AATTTTACTG 
AGGCGTACCA CGACTATTAC CATAGAGTCA GAGGGAAAGG GCTCAACCTT CTCCAAGGCA 
AGTTTTGTTG CTTCTGAGAA CAGGACTGAT ATATCCCTAG ATGATCCTAA TTTCTGGCAA 
AAGTGGGCCA AGAAAGCAGA CTTAGACTTG GATCTTCTTA GCAGCAAGAA TACACTGGTT 
ATTGATACCC CACGTATCAG GAAGCAAACA CGGCACTTTA CTAACAAAGA TGATGACATG 
GTTGAGTTCT CAGATTTGGA GAGTGATGAT GATGATCGCC CCAAAGCTCG GCGTCAAGAC 
CGCAAGCATG GATATGGACG TACAGATTGC TTCCGTGTGG AGAAGCATTT GCTTGTTTAT 
GGATGGGGTC GCTGGCGAGA TATCCTTACT CATGGTCGTT TCAAGCGTGG TATGAATGAG 
CGTGACGTGG AGAAAATATG TAGAGCCATA CTGGTTTATT GTCTTTTGCA CTACCGGGGA 
GATGAAAACA TAAAGAGCTT TATCTGGGAT CTAATTACAC CAGCTGAGAA TGGCAAAACT 
AAGCAGCTAC AGAACCACTC TGGATTGTCT ATTCCTGTCC CCCGTGGTCG TAAGGGGAAA 
AAAGTGAAAT CCCAAAGCAG CTTTGACATA CATAAAGCCG ATTGGATACA TAAGTACAAC 
CCAGATACCT TGTTCCAAGA TGAAGGGTAC AAGAAACATC TCAAGCATCA ATGTAACAAG 
GTGCTGCTTC GCGTTCGTAT GTTATATTTT CTTCGTCAGG AAGTTATTGG AAACCAGGCA 
ATGAAAGTTT TAAGTGGTGT AGAGGCCAGG CAAATTGACA TCTGGTTCCC ACAGGTGGAC 
CAGGTTGAAG TTCCCTCACG CTGGTGGGAC AGAGAAGCTG ATAAATCTCT TCTTATCGGG 
GTCTTTAAAC ATGGTTATGA GAAGTATAAC ACCATGCGAG CTGACCCTGC ACTCTGTTTT 
TTGGAGAAGG TGGGGGGACC AGATGAACAG GCTATTGCAG CTGAGCACCA TGCTCAAGAC 
TTTTCTGAAC TCCCTGATGG TGGGGACTTT GATCGAGACA TAGAAGATCC AGAATACAAG 
CCACTTCATG CACAGAAAGA TCCTGAGGAT GAGATCGACT CTTTAATGAT GGATGAAGAA 
ATCTCTGTTG TTGACGGTGA AGAAGCAACA GCAATGCCCA GTGGTATGTT ATGGCCACCT 
GGCTCTGCTC TCACAGCTCG TCTACGGCGT CTAATTACTG CCTACCAACG CAGCTACAAA 
AGAGAACAAC TAAAATTGGA GGCTGAAGAG CGTGGGGACA AACGTAGAAA ACGATGTGAG 
GCAGCCTTTA AACTGAAAGA AATTGCCCGT AGGGAGAAAC AGCAAAGGTG GACCCGAAGG 
GAGGCATGTG ACTTTTTTCG TGTGCTGTCC AGCTTTGGTG TCGAGTATGA CCCCGATACT 
CAACTGTATG ACTGGCAGAG GTTCCGTGGT CTGGCAAGAC TGGACAAGAA GACAGATGAG 
TCACTGCTCA AATACTTCCA TGGATTTGTG GCAATGTGCC GCCAGGTTTG CCGCTTACCC 
CCTGCTGCCG GAGACGAGCC ACCAGATCCA TCTCTCTTCA TTGAGCCAAT TTCAGAAGAG 
CGAGCATCTC GCGCCCTGTT TCGCTTGGAT CTTCTTCGTA GGGTGCGTGA GCAAGTACTT 
TGTCATCCTT TGCTTATTCC TCGTTTGTCA CTGTGCCGGC CAGACCCAGA ACTCCCAGAA 
TGGTGGGAGA GTGGACGTCA TGACAATGAG CTTCTTCAGG GAGCAGCACG TTATGGTCTG 
AGCCGTACAG ACCTCACTAT ACTGCAGGAC CATGCATTTT CTTTTTTGCG GTGTCAGATC 
AGTTATTTCC AGTCAAGGGG AATATCTGGC CATTCTCGCC CTTCTACTCC AACTACTGCC 
CCTTCAGTGC CTACTGAACG TCAGCCTTCT CATCTAGCCT CCCTTTCCTC ATCTGTCAGC 
TGTTCTCCTG CTCCACGCAG ATCCTCTTCC TGCTCATCAT CATCTCACTC ATCAAACTCT 
GAGGATTCTT CAGATGAAAG CAATGGTGTA AAAGTAAAAC CTAATGCAGG TCTTTCGCAC 
GCTCGACTGT ATGATGAGGA GAGCCGGCTT TCTCTTACTG CCTCCCCTGC AGATCTCACT 
ACAGAGGACA GTATTCAGAC AGCGCTGGAG ACCCCGCACA GTACAGACTG GCCAAAGGAC 
CGTGTCCTTA TCTGCAGAAT TGAGCAAGTA TGCAGTGCCG TTCTTACTGG CAAATGGTCC 
TCACCACGAC GACTGTCAGA TCCACCAAGT GACAGCCCTG ATAGCTTGCC TCCAACCCCA 
GAGCAGCAGA GTCCAGCCCA CTTCACACAG ATAAGACCTG CACCGGACCC CAAAGAGTTT 
ACCATTCAAA TAAAAAGTGA AGAAGGCCTG AAGATTAAAT TTCAGAAGCA CAAATTTATG 
GGTAATGGAG CTCTTGATTC TGCACCTCAT ACCCAGAAGA AAAAGAGCAA GAAAAAGATG 
GATCTTGACA TGGACACACG AATTCCCGTT GTAAACCAGA AGGATGGGAC CTTGTTGTTA 
GGGGAAGAAG CCCCTTTACG CTGCCAGCTG CCCGAGTGGT TACACAGAAA TCCTGACTTC 
ATGGTTGACC CACGCTTTAT TGCGTTTATG GAAGACCGCA GGAAAAGAAA AAAACATCGA 
AGCAAAAAGT CCAAATCCTC CTCCCAGATT GGAAACGTAC AGAATCCCCA CTCCCACAAT 
GGAAAGAAGT CTTCCCACAC AGAAAACTCT TTCGAGACGC AACACCTTCC TACTGCAGCC 
AAAAAAAGGA AGAAACGCCC TCCTTCAAAA ATAATAATGC CATCTGCACT ACATGGAGCT 
GGTGATGTTA CACATTCCTT CTCAGACCTT CAACCTGACC ATTCTGGTCA CCCCATGCGG 
TCAGTGTATG GACCATCAGA TGGAGGGCTG TCCCATCAAG CAGGACTCAG CGGGTTGGGA 
TTTACCCGCT TTCATTCATC GCAGACATCT TCAGTGGGCA GAATCCATGG CACTACCTTT 
GATGAGTTAC AAAGTTTTGA GCCCCCACAA CAACATTTTT CTCTGCTTGA TGATCCTATG 
ATGCCAGCAA ATTCCTCCTC CAGCGGTGAT GAACTGGACA GACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chd8 ( XM_012957178.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012812632.1 Xenopus tropicalis chromodomain helicase DNA binding protein 8 (chd8), transcript variant X2, mRNA.

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CloneID	OXa32500
Clone ID Related Accession (Same CDS sequence)	XM_012957172.3 , XM_012957178.3 , XM_031896082.1 , XM_012957178.2 , XM_012957172.2 , XM_012957182.2 , XM_012957182.3
Accession Version	XM_012957178.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7008bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	chromodomain-helicase-DNA-binding protein 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957178.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACC CTATCATGGA CCTCTTTGAT GATCCAAACC TATTTGGCCT TGACTCTCTC 
ACTGGAGATT CCTTTGGACG GGATGGGCCA GACACTATAG ATGATGCTCT AGGCCTGGGT 
AATGTCTTGG GACCTTTGGA GCCGATAACA GACAGAGTTG GCCTGCCTGG TGCTGATGTT 
GGCAATAATG AAGTTAAACA ACAAACAGAG TCACAGGTTC TCCCGGACAA TCCAGCTCTT 
ATGTCAGCTA CTGAGATCAT GCAGCCCATT CAACTTCCCA CAAACCAGGA AACACTGAAC 
CAAGGAAATC CCTTTATGGG TGCCTCAAAT GCTGGAGCCC CAAAAATTAT GATTCTGAAA 
GCTCCTGCTG GAATGACAGT TGGTGCATGT CCTGTAACTC AGATACAAAC TCTGACACCT 
CACCAAGCTG CTAATGGTGG CAAGGTGGCA TTTGCTAAGG TGCTTACAGG AACCCCATTG 
CGTCCTGGGG TCTCAATTGT ATCTGGAAAC ACTGTCCTTG CCAAAATGCC AAGCACTGGA 
GTTGCAGGGC AGGTAGGAGC AGTGAGGCCT GTCCGTCAGC TACTTCTTCA ACCAGTGCGT 
GCCTCCGCAG CCCCAGGAAG CTCCGAGAGT AATACAGGGA TCAAGCCTGC AATCACACTA 
ACGTCAGCAC CTCAACAGGG GGACCCTAAA AGAATTACTC TCGTCCTACA GCAGCCTTCC 
CAAGTTGGTG CAACTGCACA ACAAGGGCAG CGTCATGTTG TGCTGGGTGG GCTTCCAGGA 
AAGATTGTCC TTCAGGGAAA CCAACTGGCA GCTCTCACAC AAGCTAAAAC CCCTCAAGGT 
CAGCCAGCTA AAGTAGTGAC TATCCAGCTG CAAGTCCAGC AGCAGCCTGG GGCTGCAGGT 
CAGACTCCTC AAAAGTTTCA GATTGTACAA CAAGCCCCAG GAGGTGTGGC AATTGCTCCC 
AGTGGGCAAC ATACACACAT GCTTAATCAA CAAGGAGTTC AGAGGCTTTC TGTTCCACTC 
AAAGTTGTTC TGCAACCACA GGCTGGTTCC TCTCAGGGTA CTGCGCAGGG CCTGTCAGTT 
GTGAAAGTTC TAAATGCAAG TGAAGTTGCC AATTTGACTG CAAGTCAGAC TGTGGTTAAA 
ACATCCACTG GAGGTGGTGA ATCTCGTAAG CTGGACAGCC AGAAGAAGCA AGAGAAAGCT 
AACCGTATTG TAGCTGAAGC TATTGCCAGG GCCCGAGCCA GAGGTGAACA GAATATTCCT 
CGGGTCCTGA ATGAGGATGA GCTTCCCAGT GTGAATCCTG AAGATGATGA TGGAAGCAGG 
CGGAAAAGGA AGAAGAAGAA AGGAGAGACT AGTGATAGGT CCAAGGATGA GAAACCTAAG 
AAGGTCAAAG GTAGTGGAAG CTTAAGGTCT AGGAGCAAAG GCAAACCAAG TACTATCACT 
CCTATTGTGG GTAAGAAAAG GAAGAGAAAC CCATCTTCCG ATAATTCTGA TGCTGAGATC 
ATGGCATCAC AGGCTTCTCC TGCAGAGGAT GAAGACAGTG TCCAGAAGAG ACGTTCAAAT 
CGCCAGGTGA AGCGCAAGAA GTATACAGAG GATTTGGATA TTAAAATAAC AGATGATGAA 
GATGATGAGG AGGTAGATGT GACGGGACCT GTGAAAACAG AACCTGTGCT GCTTCCTGAG 
CCCATGCCTT TACCTGACAG TGAGGCAGTC CCTTCAATGC AGTTCTTCGT GGAGAACCCA 
AGTGAAGAAG ATGCTGCTAT TGTTGACAAA ATACTCTCTA TGCGCCTAGC TAAAAAAGAG 
CTGCCCACTG GAGAGTATGT TGAAGTTGAA GAGTACTTTG TCAAATATAA AAATTATTCC 
TACCTACACT GTGAATGGGC CACAATTGAG CAACTGGAAA GGGACAAGCG TATTCATCAG 
AAACTGAAAA GATTTAAGAC AAAGATGACC CAGATGCAGC ATTTCTTGCA AGAAGATGAA 
GAATCTTTCA ATCCAGACTA TGTAGAGGTA GATCGAATCC TAGACGAATC TCACAGCACT 
GACAAGGACA ATGGTGAACC GGTGGTGTAT TACCTAGTAA AATGGTGCTC TTTACCTTAT 
GAAGATAGCA CATGGGAGCT GAAAGAAGAT GTTGATGATG GAAAGATAGA GGAATTCAAA 
CGAATAGAGG CGAGGCAGCC AAATCTCAAA CGCGTGGCCC GCCCAGCTGC TACATCATGG 
AAGAAGCTGG AATTGTCTCG GGAGTATCAG AATGGCAACC AGTTGAGAGA ATACCAGCTG 
GAAGGTGTTA ATTGGCTTCT CTTTAACTGG TACAATAGGC AAAACTGCAT TTTGGCAGAT 
GAAATGGGTT TGGGGAAGAC CATCCAATCT ATCACATTCT TACAAGAAGT ATATAATGTG 
GGAATACGGG GACCTTTTTT AGTAATTGCT CCACTTTCCA CCATCACGAA CTGGGAGAGA 
GAGTTTGGCA GTTGGACTCA AATGAATACT ATTGTGTATC ATGGCAGCCT TGCTAGTCGC 
CAAATGATCC AGCAATATGA GATGTACTGT AAGGACTCCA AGGGTCGTCT TATTCCTGGT 
GCGTACAAGT TTGATGCTCT TATTACAACT TTTGAAATGG TGCTGTCTGA TTGCCCAGAG 
CTGCGTGAAA TTGAGTGGCG CTGTGTCATA ATTGATGAGG CTCATCGGCT CAAAAACAGA 
AACTGCAAAC TACTGGACAG CCTAAAGCAC ATGGACTTGG AGCACAAAGT CCTTTTAACA 
GGCACTCCAT TACAGAACAC CGTGGAAGAG TTGTTCAGTC TTTTGCATTT CCTGGAGCCA 
ACACAATTTT CCTCTGAAGC AGAGTTTCTT AAGGACTTTG GAGACCTGAA GACAGAGGAA 
CAGGTGCAGA AATTACAGGC CATCTTAAAA CCTATGATGT TGAGGCGATT AAAGGAAGAT 
GTTGAGAAAA ACCTGGCCCC GAAACAAGAA ACTATAATTG AGGTTGAGCT TACTAACATC 
CAGAAGAAGT ATTACCGAGC AATACTGGAG AAGAATTTTT CTTTTCTGAC CAAAGGAGCT 
AGTCAATCCA ACACACCCAA CTTGCTCAAT ACCATGATGG AACTACGCAA ATGCTGCAAC 
CACCCATACC TCATCACTGG AGCAGAAGAA AAGATCATAT CTGAGTTCCG AGAAGCAACT 
CCAGTAGTTC CCCCTGACTT TCACGTCCAA GCAATGGTAC GCTCCTCTGG CAAACTGGTG 
CTAATAGATA AGCTGCTCCC TAAACTAAGG GCTGGAGGCC ACAAAGTTCT CATCTTTTCT 
CAGATGGTAC GGTGCCTGGA CATCCTGGAA GACTATCTCA TTCAGAGAAG GTACCTTTAT 
GAGCGTATTG ATGGACGTGT CCGTGGCAAC ATGCGTCAGG CAGCCATTGA TAGATTCAGC 
CGCCCAGATT CTGACCGATT TGTGTTTCTA CTCTGTACAC GAGCTGGTGG GCTTGGAATT 
AACTTGACAG CTGCTGATAC TTGTATTATA TTTGACTCGG ACTGGAACCC TCAAAATGAC 
CTTCAGGCTC AAGCTCGATG TCATCGTATT GGGCAAAGCA AAGCCGTTAA AATTTACAGA 
CTCATCACTC GCAACTCCTA TGAGAGAGAG ATGTTTGATA AGGCCAGTCT CAAACTAGGA 
CTAGACAAAG CTGTATTGCA GTCTATGAGT GGTCGGGATA ATCACCTTAG TGGGCCTATT 
CAGCAATTCA CAAAGAAAGA AATTGAAGAC TTGCTAAGGA AAGGAGCTTA TGCTGCAATC 
ATGGATGAAG ATGACGAAGG CTCTAAATTC TGTGAAGAAG ATATTGACCA AATTTTACTG 
AGGCGTACCA CGACTATTAC CATAGAGTCA GAGGGAAAGG GCTCAACCTT CTCCAAGGCA 
AGTTTTGTTG CTTCTGAGAA CAGGACTGAT ATATCCCTAG ATGATCCTAA TTTCTGGCAA 
AAGTGGGCCA AGAAAGCAGA CTTAGACTTG GATCTTCTTA GCAGCAAGAA TACACTGGTT 
ATTGATACCC CACGTATCAG GAAGCAAACA CGGCACTTTA CTAACAAAGA TGATGACATG 
GTTGAGTTCT CAGATTTGGA GAGTGATGAT GATGATCGCC CCAAAGCTCG GCGTCAAGAC 
CGCAAGCATG GATATGGACG TACAGATTGC TTCCGTGTGG AGAAGCATTT GCTTGTTTAT 
GGATGGGGTC GCTGGCGAGA TATCCTTACT CATGGTCGTT TCAAGCGTGG TATGAATGAG 
CGTGACGTGG AGAAAATATG TAGAGCCATA CTGGTTTATT GTCTTTTGCA CTACCGGGGA 
GATGAAAACA TAAAGAGCTT TATCTGGGAT CTAATTACAC CAGCTGAGAA TGGCAAAACT 
AAGCAGCTAC AGAACCACTC TGGATTGTCT ATTCCTGTCC CCCGTGGTCG TAAGGGGAAA 
AAAGTGAAAT CCCAAAGCAG CTTTGACATA CATAAAGCCG ATTGGATACA TAAGTACAAC 
CCAGATACCT TGTTCCAAGA TGAAGGGTAC AAGAAACATC TCAAGCATCA ATGTAACAAG 
GTGCTGCTTC GCGTTCGTAT GTTATATTTT CTTCGTCAGG AAGTTATTGG AAACCAGGCA 
ATGAAAGTTT TAAGTGGTGT AGAGGCCAGG CAAATTGACA TCTGGTTCCC ACAGGTGGAC 
CAGGTTGAAG TTCCCTCACG CTGGTGGGAC AGAGAAGCTG ATAAATCTCT TCTTATCGGG 
GTCTTTAAAC ATGGTTATGA GAAGTATAAC ACCATGCGAG CTGACCCTGC ACTCTGTTTT 
TTGGAGAAGG TGGGGGGACC AGATGAACAG GCTATTGCAG CTGAGCACCA TGCTCAAGAC 
TTTTCTGAAC TCCCTGATGG TGGGGACTTT GATCGAGACA TAGAAGATCC AGAATACAAG 
CCACTTCATG CACAGAAAGA TCCTGAGGAT GAGATCGACT CTTTAATGAT GGATGAAGAA 
ATCTCTGTTG TTGACGGTGA AGAAGCAACA GCAATGCCCA GTGGTATGTT ATGGCCACCT 
GGCTCTGCTC TCACAGCTCG TCTACGGCGT CTAATTACTG CCTACCAACG CAGCTACAAA 
AGAGAACAAC TAAAATTGGA GGCTGAAGAG CGTGGGGACA AACGTAGAAA ACGATGTGAG 
GCAGCCTTTA AACTGAAAGA AATTGCCCGT AGGGAGAAAC AGCAAAGGTG GACCCGAAGG 
GAGGCATGTG ACTTTTTTCG TGTGCTGTCC AGCTTTGGTG TCGAGTATGA CCCCGATACT 
CAACTGTATG ACTGGCAGAG GTTCCGTGGT CTGGCAAGAC TGGACAAGAA GACAGATGAG 
TCACTGCTCA AATACTTCCA TGGATTTGTG GCAATGTGCC GCCAGGTTTG CCGCTTACCC 
CCTGCTGCCG GAGACGAGCC ACCAGATCCA TCTCTCTTCA TTGAGCCAAT TTCAGAAGAG 
CGAGCATCTC GCGCCCTGTT TCGCTTGGAT CTTCTTCGTA GGGTGCGTGA GCAAGTACTT 
TGTCATCCTT TGCTTATTCC TCGTTTGTCA CTGTGCCGGC CAGACCCAGA ACTCCCAGAA 
TGGTGGGAGA GTGGACGTCA TGACAATGAG CTTCTTCAGG GAGCAGCACG TTATGGTCTG 
AGCCGTACAG ACCTCACTAT ACTGCAGGAC CATGCATTTT CTTTTTTGCG GTGTCAGATC 
AGTTATTTCC AGTCAAGGGG AATATCTGGC CATTCTCGCC CTTCTACTCC AACTACTGCC 
CCTTCAGTGC CTACTGAACG TCAGCCTTCT CATCTAGCCT CCCTTTCCTC ATCTGTCAGC 
TGTTCTCCTG CTCCACGCAG ATCCTCTTCC TGCTCATCAT CATCTCACTC ATCAAACTCT 
GAGGATTCTT CAGATGAAAG CAATGGTGTA AAAGTAAAAC CTAATGCAGG TCTTTCGCAC 
GCTCGACTGT ATGATGAGGA GAGCCGGCTT TCTCTTACTG CCTCCCCTGC AGATCTCACT 
ACAGAGGACA GTATTCAGAC AGCGCTGGAG ACCCCGCACA GTACAGACTG GCCAAAGGAC 
CGTGTCCTTA TCTGCAGAAT TGAGCAAGTA TGCAGTGCCG TTCTTACTGG CAAATGGTCC 
TCACCACGAC GACTGTCAGA TCCACCAAGT GACAGCCCTG ATAGCTTGCC TCCAACCCCA 
GAGCAGCAGA GTCCAGCCCA CTTCACACAG ATAAGACCTG CACCGGACCC CAAAGAGTTT 
ACCATTCAAA TAAAAAGTGA AGAAGGCCTG AAGATTAAAT TTCAGAAGCA CAAATTTATG 
GGTAATGGAG CTCTTGATTC TGCACCTCAT ACCCAGAAGA AAAAGAGCAA GAAAAAGATG 
GATCTTGACA TGGACACACG AATTCCCGTT GTAAACCAGA AGGATGGGAC CTTGTTGTTA 
GGGGAAGAAG CCCCTTTACG CTGCCAGCTG CCCGAGTGGT TACACAGAAA TCCTGACTTC 
ATGGTTGACC CACGCTTTAT TGCGTTTATG GAAGACCGCA GGAAAAGAAA AAAACATCGA 
AGCAAAAAGT CCAAATCCTC CTCCCAGATT GGAAACGTAC AGAATCCCCA CTCCCACAAT 
GGAAAGAAGT CTTCCCACAC AGAAAACTCT TTCGAGACGC AACACCTTCC TACTGCAGCC 
AAAAAAAGGA AGAAACGCCC TCCTTCAAAA ATAATAATGC CATCTGCACT ACATGGAGCT 
GGTGATGTTA CACATTCCTT CTCAGACCTT CAACCTGACC ATTCTGGTCA CCCCATGCGG 
TCAGTGTATG GACCATCAGA TGGAGGGCTG TCCCATCAAG CAGGACTCAG CGGGTTGGGA 
TTTACCCGCT TTCATTCATC GCAGACATCT TCAGTGGGCA GAATCCATGG CACTACCTTT 
GATGAGTTAC AAAGTTTTGA GCCCCCACAA CAACATTTTT CTCTGCTTGA TGATCCTATG 
ATGCCAGCAA ATTCCTCCTC CAGCGGTGAT GAACTGGACA GACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012812632.1
CDS	260..7267
Translation	MADPIMDLFDDPNLFGLDSLTGDSFGRDGPDTIDDALGLGNVLGPLEPITDRVGLPGADVGNNEVKQQTESQVLPDNPALMSATEIMQPIQLPTNQETLNQGNPFMGASNAGAPKIMILKAPAGMTVGACPVTQIQTLTPHQAANGGKVAFAKVLTGTPLRPGVSIVSGNTVLAKMPSTGVAGQVGAVRPVRQLLLQPVRASAAPGSSESNTGIKPAITLTSAPQQGDPKRITLVLQQPSQVGATAQQGQRHVVLGGLPGKIVLQGNQLAALTQAKTPQGQPAKVVTIQLQVQQQPGAAGQTPQKFQIVQQAPGGVAIAPSGQHTHMLNQQGVQRLSVPLKVVLQPQAGSSQGTAQGLSVVKVLNASEVANLTASQTVVKTSTGGGESRKLDSQKKQEKANRIVAEAIARARARGEQNIPRVLNEDELPSVNPEDDDGSRRKRKKKKGETSDRSKDEKPKKVKGSGSLRSRSKGKPSTITPIVGKKRKRNPSSDNSDAEIMASQASPAEDEDSVQKRRSNRQVKRKKYTEDLDIKITDDEDDEEVDVTGPVKTEPVLLPEPMPLPDSEAVPSMQFFVENPSEEDAAIVDKILSMRLAKKELPTGEYVEVEEYFVKYKNYSYLHCEWATIEQLERDKRIHQKLKRFKTKMTQMQHFLQEDEESFNPDYVEVDRILDESHSTDKDNGEPVVYYLVKWCSLPYEDSTWELKEDVDDGKIEEFKRIEARQPNLKRVARPAATSWKKLELSREYQNGNQLREYQLEGVNWLLFNWYNRQNCILADEMGLGKTIQSITFLQEVYNVGIRGPFLVIAPLSTITNWEREFGSWTQMNTIVYHGSLASRQMIQQYEMYCKDSKGRLIPGAYKFDALITTFEMVLSDCPELREIEWRCVIIDEAHRLKNRNCKLLDSLKHMDLEHKVLLTGTPLQNTVEELFSLLHFLEPTQFSSEAEFLKDFGDLKTEEQVQKLQAILKPMMLRRLKEDVEKNLAPKQETIIEVELTNIQKKYYRAILEKNFSFLTKGASQSNTPNLLNTMMELRKCCNHPYLITGAEEKIISEFREATPVVPPDFHVQAMVRSSGKLVLIDKLLPKLRAGGHKVLIFSQMVRCLDILEDYLIQRRYLYERIDGRVRGNMRQAAIDRFSRPDSDRFVFLLCTRAGGLGINLTAADTCIIFDSDWNPQNDLQAQARCHRIGQSKAVKIYRLITRNSYEREMFDKASLKLGLDKAVLQSMSGRDNHLSGPIQQFTKKEIEDLLRKGAYAAIMDEDDEGSKFCEEDIDQILLRRTTTITIESEGKGSTFSKASFVASENRTDISLDDPNFWQKWAKKADLDLDLLSSKNTLVIDTPRIRKQTRHFTNKDDDMVEFSDLESDDDDRPKARRQDRKHGYGRTDCFRVEKHLLVYGWGRWRDILTHGRFKRGMNERDVEKICRAILVYCLLHYRGDENIKSFIWDLITPAENGKTKQLQNHSGLSIPVPRGRKGKKVKSQSSFDIHKADWIHKYNPDTLFQDEGYKKHLKHQCNKVLLRVRMLYFLRQEVIGNQAMKVLSGVEARQIDIWFPQVDQVEVPSRWWDREADKSLLIGVFKHGYEKYNTMRADPALCFLEKVGGPDEQAIAAEHHAQDFSELPDGGDFDRDIEDPEYKPLHAQKDPEDEIDSLMMDEEISVVDGEEATAMPSGMLWPPGSALTARLRRLITAYQRSYKREQLKLEAEERGDKRRKRCEAAFKLKEIARREKQQRWTRREACDFFRVLSSFGVEYDPDTQLYDWQRFRGLARLDKKTDESLLKYFHGFVAMCRQVCRLPPAAGDEPPDPSLFIEPISEERASRALFRLDLLRRVREQVLCHPLLIPRLSLCRPDPELPEWWESGRHDNELLQGAARYGLSRTDLTILQDHAFSFLRCQISYFQSRGISGHSRPSTPTTAPSVPTERQPSHLASLSSSVSCSPAPRRSSSCSSSSHSSNSEDSSDESNGVKVKPNAGLSHARLYDEESRLSLTASPADLTTEDSIQTALETPHSTDWPKDRVLICRIEQVCSAVLTGKWSSPRRLSDPPSDSPDSLPPTPEQQSPAHFTQIRPAPDPKEFTIQIKSEEGLKIKFQKHKFMGNGALDSAPHTQKKKSKKKMDLDMDTRIPVVNQKDGTLLLGEEAPLRCQLPEWLHRNPDFMVDPRFIAFMEDRRKRKKHRSKKSKSSSQIGNVQNPHSHNGKKSSHTENSFETQHLPTAAKKRKKRPPSKIIMPSALHGAGDVTHSFSDLQPDHSGHPMRSVYGPSDGGLSHQAGLSGLGFTRFHSSQTSSVGRIHGTTFDELQSFEPPQQHFSLLDDPMMPANSSSSGDELDRP

Target ORF information:

RefSeq Version	XM_012957178.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis chromodomain helicase DNA binding protein 8 (chd8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957178.3

ORF Insert Sequence:

ATGGCTGACC CTATCATGGA CCTCTTTGAT GATCCAAACC TATTTGGCCT TGACTCTCTC 
ACTGGAGATT CCTTTGGACG GGATGGGCCA GACACTATAG ATGATGCTCT AGGCCTGGGT 
AATGTCTTGG GACCTTTGGA GCCGATAACA GACAGAGTTG GCCTGCCTGG TGCTGATGTT 
GGCAATAATG AAGTTAAACA ACAAACAGAG TCACAGGTTC TCCCGGACAA TCCAGCTCTT 
ATGTCAGCTA CTGAGATCAT GCAGCCCATT CAACTTCCCA CAAACCAGGA AACACTGAAC 
CAAGGAAATC CCTTTATGGG TGCCTCAAAT GCTGGAGCCC CAAAAATTAT GATTCTGAAA 
GCTCCTGCTG GAATGACAGT TGGTGCATGT CCTGTAACTC AGATACAAAC TCTGACACCT 
CACCAAGCTG CTAATGGTGG CAAGGTGGCA TTTGCTAAGG TGCTTACAGG AACCCCATTG 
CGTCCTGGGG TCTCAATTGT ATCTGGAAAC ACTGTCCTTG CCAAAATGCC AAGCACTGGA 
GTTGCAGGGC AGGTAGGAGC AGTGAGGCCT GTCCGTCAGC TACTTCTTCA ACCAGTGCGT 
GCCTCCGCAG CCCCAGGAAG CTCCGAGAGT AATACAGGGA TCAAGCCTGC AATCACACTA 
ACGTCAGCAC CTCAACAGGG GGACCCTAAA AGAATTACTC TCGTCCTACA GCAGCCTTCC 
CAAGTTGGTG CAACTGCACA ACAAGGGCAG CGTCATGTTG TGCTGGGTGG GCTTCCAGGA 
AAGATTGTCC TTCAGGGAAA CCAACTGGCA GCTCTCACAC AAGCTAAAAC CCCTCAAGGT 
CAGCCAGCTA AAGTAGTGAC TATCCAGCTG CAAGTCCAGC AGCAGCCTGG GGCTGCAGGT 
CAGACTCCTC AAAAGTTTCA GATTGTACAA CAAGCCCCAG GAGGTGTGGC AATTGCTCCC 
AGTGGGCAAC ATACACACAT GCTTAATCAA CAAGGAGTTC AGAGGCTTTC TGTTCCACTC 
AAAGTTGTTC TGCAACCACA GGCTGGTTCC TCTCAGGGTA CTGCGCAGGG CCTGTCAGTT 
GTGAAAGTTC TAAATGCAAG TGAAGTTGCC AATTTGACTG CAAGTCAGAC TGTGGTTAAA 
ACATCCACTG GAGGTGGTGA ATCTCGTAAG CTGGACAGCC AGAAGAAGCA AGAGAAAGCT 
AACCGTATTG TAGCTGAAGC TATTGCCAGG GCCCGAGCCA GAGGTGAACA GAATATTCCT 
CGGGTCCTGA ATGAGGATGA GCTTCCCAGT GTGAATCCTG AAGATGATGA TGGAAGCAGG 
CGGAAAAGGA AGAAGAAGAA AGGAGAGACT AGTGATAGGT CCAAGGATGA GAAACCTAAG 
AAGGTCAAAG GTAGTGGAAG CTTAAGGTCT AGGAGCAAAG GCAAACCAAG TACTATCACT 
CCTATTGTGG GTAAGAAAAG GAAGAGAAAC CCATCTTCCG ATAATTCTGA TGCTGAGATC 
ATGGCATCAC AGGCTTCTCC TGCAGAGGAT GAAGACAGTG TCCAGAAGAG ACGTTCAAAT 
CGCCAGGTGA AGCGCAAGAA GTATACAGAG GATTTGGATA TTAAAATAAC AGATGATGAA 
GATGATGAGG AGGTAGATGT GACGGGACCT GTGAAAACAG AACCTGTGCT GCTTCCTGAG 
CCCATGCCTT TACCTGACAG TGAGGCAGTC CCTTCAATGC AGTTCTTCGT GGAGAACCCA 
AGTGAAGAAG ATGCTGCTAT TGTTGACAAA ATACTCTCTA TGCGCCTAGC TAAAAAAGAG 
CTGCCCACTG GAGAGTATGT TGAAGTTGAA GAGTACTTTG TCAAATATAA AAATTATTCC 
TACCTACACT GTGAATGGGC CACAATTGAG CAACTGGAAA GGGACAAGCG TATTCATCAG 
AAACTGAAAA GATTTAAGAC AAAGATGACC CAGATGCAGC ATTTCTTGCA AGAAGATGAA 
GAATCTTTCA ATCCAGACTA TGTAGAGGTA GATCGAATCC TAGACGAATC TCACAGCACT 
GACAAGGACA ATGGTGAACC GGTGGTGTAT TACCTAGTAA AATGGTGCTC TTTACCTTAT 
GAAGATAGCA CATGGGAGCT GAAAGAAGAT GTTGATGATG GAAAGATAGA GGAATTCAAA 
CGAATAGAGG CGAGGCAGCC AAATCTCAAA CGCGTGGCCC GCCCAGCTGC TACATCATGG 
AAGAAGCTGG AATTGTCTCG GGAGTATCAG AATGGCAACC AGTTGAGAGA ATACCAGCTG 
GAAGGTGTTA ATTGGCTTCT CTTTAACTGG TACAATAGGC AAAACTGCAT TTTGGCAGAT 
GAAATGGGTT TGGGGAAGAC CATCCAATCT ATCACATTCT TACAAGAAGT ATATAATGTG 
GGAATACGGG GACCTTTTTT AGTAATTGCT CCACTTTCCA CCATCACGAA CTGGGAGAGA 
GAGTTTGGCA GTTGGACTCA AATGAATACT ATTGTGTATC ATGGCAGCCT TGCTAGTCGC 
CAAATGATCC AGCAATATGA GATGTACTGT AAGGACTCCA AGGGTCGTCT TATTCCTGGT 
GCGTACAAGT TTGATGCTCT TATTACAACT TTTGAAATGG TGCTGTCTGA TTGCCCAGAG 
CTGCGTGAAA TTGAGTGGCG CTGTGTCATA ATTGATGAGG CTCATCGGCT CAAAAACAGA 
AACTGCAAAC TACTGGACAG CCTAAAGCAC ATGGACTTGG AGCACAAAGT CCTTTTAACA 
GGCACTCCAT TACAGAACAC CGTGGAAGAG TTGTTCAGTC TTTTGCATTT CCTGGAGCCA 
ACACAATTTT CCTCTGAAGC AGAGTTTCTT AAGGACTTTG GAGACCTGAA GACAGAGGAA 
CAGGTGCAGA AATTACAGGC CATCTTAAAA CCTATGATGT TGAGGCGATT AAAGGAAGAT 
GTTGAGAAAA ACCTGGCCCC GAAACAAGAA ACTATAATTG AGGTTGAGCT TACTAACATC 
CAGAAGAAGT ATTACCGAGC AATACTGGAG AAGAATTTTT CTTTTCTGAC CAAAGGAGCT 
AGTCAATCCA ACACACCCAA CTTGCTCAAT ACCATGATGG AACTACGCAA ATGCTGCAAC 
CACCCATACC TCATCACTGG AGCAGAAGAA AAGATCATAT CTGAGTTCCG AGAAGCAACT 
CCAGTAGTTC CCCCTGACTT TCACGTCCAA GCAATGGTAC GCTCCTCTGG CAAACTGGTG 
CTAATAGATA AGCTGCTCCC TAAACTAAGG GCTGGAGGCC ACAAAGTTCT CATCTTTTCT 
CAGATGGTAC GGTGCCTGGA CATCCTGGAA GACTATCTCA TTCAGAGAAG GTACCTTTAT 
GAGCGTATTG ATGGACGTGT CCGTGGCAAC ATGCGTCAGG CAGCCATTGA TAGATTCAGC 
CGCCCAGATT CTGACCGATT TGTGTTTCTA CTCTGTACAC GAGCTGGTGG GCTTGGAATT 
AACTTGACAG CTGCTGATAC TTGTATTATA TTTGACTCGG ACTGGAACCC TCAAAATGAC 
CTTCAGGCTC AAGCTCGATG TCATCGTATT GGGCAAAGCA AAGCCGTTAA AATTTACAGA 
CTCATCACTC GCAACTCCTA TGAGAGAGAG ATGTTTGATA AGGCCAGTCT CAAACTAGGA 
CTAGACAAAG CTGTATTGCA GTCTATGAGT GGTCGGGATA ATCACCTTAG TGGGCCTATT 
CAGCAATTCA CAAAGAAAGA AATTGAAGAC TTGCTAAGGA AAGGAGCTTA TGCTGCAATC 
ATGGATGAAG ATGACGAAGG CTCTAAATTC TGTGAAGAAG ATATTGACCA AATTTTACTG 
AGGCGTACCA CGACTATTAC CATAGAGTCA GAGGGAAAGG GCTCAACCTT CTCCAAGGCA 
AGTTTTGTTG CTTCTGAGAA CAGGACTGAT ATATCCCTAG ATGATCCTAA TTTCTGGCAA 
AAGTGGGCCA AGAAAGCAGA CTTAGACTTG GATCTTCTTA GCAGCAAGAA TACACTGGTT 
ATTGATACCC CACGTATCAG GAAGCAAACA CGGCACTTTA CTAACAAAGA TGATGACATG 
GTTGAGTTCT CAGATTTGGA GAGTGATGAT GATGATCGCC CCAAAGCTCG GCGTCAAGAC 
CGCAAGCATG GATATGGACG TACAGATTGC TTCCGTGTGG AGAAGCATTT GCTTGTTTAT 
GGATGGGGTC GCTGGCGAGA TATCCTTACT CATGGTCGTT TCAAGCGTGG TATGAATGAG 
CGTGACGTGG AGAAAATATG TAGAGCCATA CTGGTTTATT GTCTTTTGCA CTACCGGGGA 
GATGAAAACA TAAAGAGCTT TATCTGGGAT CTAATTACAC CAGCTGAGAA TGGCAAAACT 
AAGCAGCTAC AGAACCACTC TGGATTGTCT ATTCCTGTCC CCCGTGGTCG TAAGGGGAAA 
AAAGTGAAAT CCCAAAGCAG CTTTGACATA CATAAAGCCG ATTGGATACA TAAGTACAAC 
CCAGATACCT TGTTCCAAGA TGAAGGGTAC AAGAAACATC TCAAGCATCA ATGTAACAAG 
GTGCTGCTTC GCGTTCGTAT GTTATATTTT CTTCGTCAGG AAGTTATTGG AAACCAGGCA 
ATGAAAGTTT TAAGTGGTGT AGAGGCCAGG CAAATTGACA TCTGGTTCCC ACAGGTGGAC 
CAGGTTGAAG TTCCCTCACG CTGGTGGGAC AGAGAAGCTG ATAAATCTCT TCTTATCGGG 
GTCTTTAAAC ATGGTTATGA GAAGTATAAC ACCATGCGAG CTGACCCTGC ACTCTGTTTT 
TTGGAGAAGG TGGGGGGACC AGATGAACAG GCTATTGCAG CTGAGCACCA TGCTCAAGAC 
TTTTCTGAAC TCCCTGATGG TGGGGACTTT GATCGAGACA TAGAAGATCC AGAATACAAG 
CCACTTCATG CACAGAAAGA TCCTGAGGAT GAGATCGACT CTTTAATGAT GGATGAAGAA 
ATCTCTGTTG TTGACGGTGA AGAAGCAACA GCAATGCCCA GTGGTATGTT ATGGCCACCT 
GGCTCTGCTC TCACAGCTCG TCTACGGCGT CTAATTACTG CCTACCAACG CAGCTACAAA 
AGAGAACAAC TAAAATTGGA GGCTGAAGAG CGTGGGGACA AACGTAGAAA ACGATGTGAG 
GCAGCCTTTA AACTGAAAGA AATTGCCCGT AGGGAGAAAC AGCAAAGGTG GACCCGAAGG 
GAGGCATGTG ACTTTTTTCG TGTGCTGTCC AGCTTTGGTG TCGAGTATGA CCCCGATACT 
CAACTGTATG ACTGGCAGAG GTTCCGTGGT CTGGCAAGAC TGGACAAGAA GACAGATGAG 
TCACTGCTCA AATACTTCCA TGGATTTGTG GCAATGTGCC GCCAGGTTTG CCGCTTACCC 
CCTGCTGCCG GAGACGAGCC ACCAGATCCA TCTCTCTTCA TTGAGCCAAT TTCAGAAGAG 
CGAGCATCTC GCGCCCTGTT TCGCTTGGAT CTTCTTCGTA GGGTGCGTGA GCAAGTACTT 
TGTCATCCTT TGCTTATTCC TCGTTTGTCA CTGTGCCGGC CAGACCCAGA ACTCCCAGAA 
TGGTGGGAGA GTGGACGTCA TGACAATGAG CTTCTTCAGG GAGCAGCACG TTATGGTCTG 
AGCCGTACAG ACCTCACTAT ACTGCAGGAC CATGCATTTT CTTTTTTGCG GTGTCAGATC 
AGTTATTTCC AGTCAAGGGG AATATCTGGC CATTCTCGCC CTTCTACTCC AACTACTGCC 
CCTTCAGTGC CTACTGAACG TCAGCCTTCT CATCTAGCCT CCCTTTCCTC ATCTGTCAGC 
TGTTCTCCTG CTCCACGCAG ATCCTCTTCC TGCTCATCAT CATCTCACTC ATCAAACTCT 
GAGGATTCTT CAGATGAAAG CAATGGTGTA AAAGTAAAAC CTAATGCAGG TCTTTCGCAC 
GCTCGACTGT ATGATGAGGA GAGCCGGCTT TCTCTTACTG CCTCCCCTGC AGATCTCACT 
ACAGAGGACA GTATTCAGAC AGCGCTGGAG ACCCCGCACA GTACAGACTG GCCAAAGGAC 
CGTGTCCTTA TCTGCAGAAT TGAGCAAGTA TGCAGTGCCG TTCTTACTGG CAAATGGTCC 
TCACCACGAC GACTGTCAGA TCCACCAAGT GACAGCCCTG ATAGCTTGCC TCCAACCCCA 
GAGCAGCAGA GTCCAGCCCA CTTCACACAG ATAAGACCTG CACCGGACCC CAAAGAGTTT 
ACCATTCAAA TAAAAAGTGA AGAAGGCCTG AAGATTAAAT TTCAGAAGCA CAAATTTATG 
GGTAATGGAG CTCTTGATTC TGCACCTCAT ACCCAGAAGA AAAAGAGCAA GAAAAAGATG 
GATCTTGACA TGGACACACG AATTCCCGTT GTAAACCAGA AGGATGGGAC CTTGTTGTTA 
GGGGAAGAAG CCCCTTTACG CTGCCAGCTG CCCGAGTGGT TACACAGAAA TCCTGACTTC 
ATGGTTGACC CACGCTTTAT TGCGTTTATG GAAGACCGCA GGAAAAGAAA AAAACATCGA 
AGCAAAAAGT CCAAATCCTC CTCCCAGATT GGAAACGTAC AGAATCCCCA CTCCCACAAT 
GGAAAGAAGT CTTCCCACAC AGAAAACTCT TTCGAGACGC AACACCTTCC TACTGCAGCC 
AAAAAAAGGA AGAAACGCCC TCCTTCAAAA ATAATAATGC CATCTGCACT ACATGGAGCT 
GGTGATGTTA CACATTCCTT CTCAGACCTT CAACCTGACC ATTCTGGTCA CCCCATGCGG 
TCAGTGTATG GACCATCAGA TGGAGGGCTG TCCCATCAAG CAGGACTCAG CGGGTTGGGA 
TTTACCCGCT TTCATTCATC GCAGACATCT TCAGTGGGCA GAATCCATGG CACTACCTTT 
GATGAGTTAC AAAGTTTTGA GCCCCCACAA CAACATTTTT CTCTGCTTGA TGATCCTATG 
ATGCCAGCAA ATTCCTCCTC CAGCGGTGAT GAACTGGACA GACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chd8 ( XM_031896082.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751942.1 Xenopus tropicalis chromodomain helicase DNA binding protein 8 (chd8), transcript variant X4, mRNA.

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CloneID	OXa32500
Clone ID Related Accession (Same CDS sequence)	XM_012957172.3 , XM_012957178.3 , XM_031896082.1 , XM_012957178.2 , XM_012957172.2 , XM_012957182.2 , XM_012957182.3
Accession Version	XM_031896082.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7008bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	chromodomain-helicase-DNA-binding protein 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACC CTATCATGGA CCTCTTTGAT GATCCAAACC TATTTGGCCT TGACTCTCTC 
ACTGGAGATT CCTTTGGACG GGATGGGCCA GACACTATAG ATGATGCTCT AGGCCTGGGT 
AATGTCTTGG GACCTTTGGA GCCGATAACA GACAGAGTTG GCCTGCCTGG TGCTGATGTT 
GGCAATAATG AAGTTAAACA ACAAACAGAG TCACAGGTTC TCCCGGACAA TCCAGCTCTT 
ATGTCAGCTA CTGAGATCAT GCAGCCCATT CAACTTCCCA CAAACCAGGA AACACTGAAC 
CAAGGAAATC CCTTTATGGG TGCCTCAAAT GCTGGAGCCC CAAAAATTAT GATTCTGAAA 
GCTCCTGCTG GAATGACAGT TGGTGCATGT CCTGTAACTC AGATACAAAC TCTGACACCT 
CACCAAGCTG CTAATGGTGG CAAGGTGGCA TTTGCTAAGG TGCTTACAGG AACCCCATTG 
CGTCCTGGGG TCTCAATTGT ATCTGGAAAC ACTGTCCTTG CCAAAATGCC AAGCACTGGA 
GTTGCAGGGC AGGTAGGAGC AGTGAGGCCT GTCCGTCAGC TACTTCTTCA ACCAGTGCGT 
GCCTCCGCAG CCCCAGGAAG CTCCGAGAGT AATACAGGGA TCAAGCCTGC AATCACACTA 
ACGTCAGCAC CTCAACAGGG GGACCCTAAA AGAATTACTC TCGTCCTACA GCAGCCTTCC 
CAAGTTGGTG CAACTGCACA ACAAGGGCAG CGTCATGTTG TGCTGGGTGG GCTTCCAGGA 
AAGATTGTCC TTCAGGGAAA CCAACTGGCA GCTCTCACAC AAGCTAAAAC CCCTCAAGGT 
CAGCCAGCTA AAGTAGTGAC TATCCAGCTG CAAGTCCAGC AGCAGCCTGG GGCTGCAGGT 
CAGACTCCTC AAAAGTTTCA GATTGTACAA CAAGCCCCAG GAGGTGTGGC AATTGCTCCC 
AGTGGGCAAC ATACACACAT GCTTAATCAA CAAGGAGTTC AGAGGCTTTC TGTTCCACTC 
AAAGTTGTTC TGCAACCACA GGCTGGTTCC TCTCAGGGTA CTGCGCAGGG CCTGTCAGTT 
GTGAAAGTTC TAAATGCAAG TGAAGTTGCC AATTTGACTG CAAGTCAGAC TGTGGTTAAA 
ACATCCACTG GAGGTGGTGA ATCTCGTAAG CTGGACAGCC AGAAGAAGCA AGAGAAAGCT 
AACCGTATTG TAGCTGAAGC TATTGCCAGG GCCCGAGCCA GAGGTGAACA GAATATTCCT 
CGGGTCCTGA ATGAGGATGA GCTTCCCAGT GTGAATCCTG AAGATGATGA TGGAAGCAGG 
CGGAAAAGGA AGAAGAAGAA AGGAGAGACT AGTGATAGGT CCAAGGATGA GAAACCTAAG 
AAGGTCAAAG GTAGTGGAAG CTTAAGGTCT AGGAGCAAAG GCAAACCAAG TACTATCACT 
CCTATTGTGG GTAAGAAAAG GAAGAGAAAC CCATCTTCCG ATAATTCTGA TGCTGAGATC 
ATGGCATCAC AGGCTTCTCC TGCAGAGGAT GAAGACAGTG TCCAGAAGAG ACGTTCAAAT 
CGCCAGGTGA AGCGCAAGAA GTATACAGAG GATTTGGATA TTAAAATAAC AGATGATGAA 
GATGATGAGG AGGTAGATGT GACGGGACCT GTGAAAACAG AACCTGTGCT GCTTCCTGAG 
CCCATGCCTT TACCTGACAG TGAGGCAGTC CCTTCAATGC AGTTCTTCGT GGAGAACCCA 
AGTGAAGAAG ATGCTGCTAT TGTTGACAAA ATACTCTCTA TGCGCCTAGC TAAAAAAGAG 
CTGCCCACTG GAGAGTATGT TGAAGTTGAA GAGTACTTTG TCAAATATAA AAATTATTCC 
TACCTACACT GTGAATGGGC CACAATTGAG CAACTGGAAA GGGACAAGCG TATTCATCAG 
AAACTGAAAA GATTTAAGAC AAAGATGACC CAGATGCAGC ATTTCTTGCA AGAAGATGAA 
GAATCTTTCA ATCCAGACTA TGTAGAGGTA GATCGAATCC TAGACGAATC TCACAGCACT 
GACAAGGACA ATGGTGAACC GGTGGTGTAT TACCTAGTAA AATGGTGCTC TTTACCTTAT 
GAAGATAGCA CATGGGAGCT GAAAGAAGAT GTTGATGATG GAAAGATAGA GGAATTCAAA 
CGAATAGAGG CGAGGCAGCC AAATCTCAAA CGCGTGGCCC GCCCAGCTGC TACATCATGG 
AAGAAGCTGG AATTGTCTCG GGAGTATCAG AATGGCAACC AGTTGAGAGA ATACCAGCTG 
GAAGGTGTTA ATTGGCTTCT CTTTAACTGG TACAATAGGC AAAACTGCAT TTTGGCAGAT 
GAAATGGGTT TGGGGAAGAC CATCCAATCT ATCACATTCT TACAAGAAGT ATATAATGTG 
GGAATACGGG GACCTTTTTT AGTAATTGCT CCACTTTCCA CCATCACGAA CTGGGAGAGA 
GAGTTTGGCA GTTGGACTCA AATGAATACT ATTGTGTATC ATGGCAGCCT TGCTAGTCGC 
CAAATGATCC AGCAATATGA GATGTACTGT AAGGACTCCA AGGGTCGTCT TATTCCTGGT 
GCGTACAAGT TTGATGCTCT TATTACAACT TTTGAAATGG TGCTGTCTGA TTGCCCAGAG 
CTGCGTGAAA TTGAGTGGCG CTGTGTCATA ATTGATGAGG CTCATCGGCT CAAAAACAGA 
AACTGCAAAC TACTGGACAG CCTAAAGCAC ATGGACTTGG AGCACAAAGT CCTTTTAACA 
GGCACTCCAT TACAGAACAC CGTGGAAGAG TTGTTCAGTC TTTTGCATTT CCTGGAGCCA 
ACACAATTTT CCTCTGAAGC AGAGTTTCTT AAGGACTTTG GAGACCTGAA GACAGAGGAA 
CAGGTGCAGA AATTACAGGC CATCTTAAAA CCTATGATGT TGAGGCGATT AAAGGAAGAT 
GTTGAGAAAA ACCTGGCCCC GAAACAAGAA ACTATAATTG AGGTTGAGCT TACTAACATC 
CAGAAGAAGT ATTACCGAGC AATACTGGAG AAGAATTTTT CTTTTCTGAC CAAAGGAGCT 
AGTCAATCCA ACACACCCAA CTTGCTCAAT ACCATGATGG AACTACGCAA ATGCTGCAAC 
CACCCATACC TCATCACTGG AGCAGAAGAA AAGATCATAT CTGAGTTCCG AGAAGCAACT 
CCAGTAGTTC CCCCTGACTT TCACGTCCAA GCAATGGTAC GCTCCTCTGG CAAACTGGTG 
CTAATAGATA AGCTGCTCCC TAAACTAAGG GCTGGAGGCC ACAAAGTTCT CATCTTTTCT 
CAGATGGTAC GGTGCCTGGA CATCCTGGAA GACTATCTCA TTCAGAGAAG GTACCTTTAT 
GAGCGTATTG ATGGACGTGT CCGTGGCAAC ATGCGTCAGG CAGCCATTGA TAGATTCAGC 
CGCCCAGATT CTGACCGATT TGTGTTTCTA CTCTGTACAC GAGCTGGTGG GCTTGGAATT 
AACTTGACAG CTGCTGATAC TTGTATTATA TTTGACTCGG ACTGGAACCC TCAAAATGAC 
CTTCAGGCTC AAGCTCGATG TCATCGTATT GGGCAAAGCA AAGCCGTTAA AATTTACAGA 
CTCATCACTC GCAACTCCTA TGAGAGAGAG ATGTTTGATA AGGCCAGTCT CAAACTAGGA 
CTAGACAAAG CTGTATTGCA GTCTATGAGT GGTCGGGATA ATCACCTTAG TGGGCCTATT 
CAGCAATTCA CAAAGAAAGA AATTGAAGAC TTGCTAAGGA AAGGAGCTTA TGCTGCAATC 
ATGGATGAAG ATGACGAAGG CTCTAAATTC TGTGAAGAAG ATATTGACCA AATTTTACTG 
AGGCGTACCA CGACTATTAC CATAGAGTCA GAGGGAAAGG GCTCAACCTT CTCCAAGGCA 
AGTTTTGTTG CTTCTGAGAA CAGGACTGAT ATATCCCTAG ATGATCCTAA TTTCTGGCAA 
AAGTGGGCCA AGAAAGCAGA CTTAGACTTG GATCTTCTTA GCAGCAAGAA TACACTGGTT 
ATTGATACCC CACGTATCAG GAAGCAAACA CGGCACTTTA CTAACAAAGA TGATGACATG 
GTTGAGTTCT CAGATTTGGA GAGTGATGAT GATGATCGCC CCAAAGCTCG GCGTCAAGAC 
CGCAAGCATG GATATGGACG TACAGATTGC TTCCGTGTGG AGAAGCATTT GCTTGTTTAT 
GGATGGGGTC GCTGGCGAGA TATCCTTACT CATGGTCGTT TCAAGCGTGG TATGAATGAG 
CGTGACGTGG AGAAAATATG TAGAGCCATA CTGGTTTATT GTCTTTTGCA CTACCGGGGA 
GATGAAAACA TAAAGAGCTT TATCTGGGAT CTAATTACAC CAGCTGAGAA TGGCAAAACT 
AAGCAGCTAC AGAACCACTC TGGATTGTCT ATTCCTGTCC CCCGTGGTCG TAAGGGGAAA 
AAAGTGAAAT CCCAAAGCAG CTTTGACATA CATAAAGCCG ATTGGATACA TAAGTACAAC 
CCAGATACCT TGTTCCAAGA TGAAGGGTAC AAGAAACATC TCAAGCATCA ATGTAACAAG 
GTGCTGCTTC GCGTTCGTAT GTTATATTTT CTTCGTCAGG AAGTTATTGG AAACCAGGCA 
ATGAAAGTTT TAAGTGGTGT AGAGGCCAGG CAAATTGACA TCTGGTTCCC ACAGGTGGAC 
CAGGTTGAAG TTCCCTCACG CTGGTGGGAC AGAGAAGCTG ATAAATCTCT TCTTATCGGG 
GTCTTTAAAC ATGGTTATGA GAAGTATAAC ACCATGCGAG CTGACCCTGC ACTCTGTTTT 
TTGGAGAAGG TGGGGGGACC AGATGAACAG GCTATTGCAG CTGAGCACCA TGCTCAAGAC 
TTTTCTGAAC TCCCTGATGG TGGGGACTTT GATCGAGACA TAGAAGATCC AGAATACAAG 
CCACTTCATG CACAGAAAGA TCCTGAGGAT GAGATCGACT CTTTAATGAT GGATGAAGAA 
ATCTCTGTTG TTGACGGTGA AGAAGCAACA GCAATGCCCA GTGGTATGTT ATGGCCACCT 
GGCTCTGCTC TCACAGCTCG TCTACGGCGT CTAATTACTG CCTACCAACG CAGCTACAAA 
AGAGAACAAC TAAAATTGGA GGCTGAAGAG CGTGGGGACA AACGTAGAAA ACGATGTGAG 
GCAGCCTTTA AACTGAAAGA AATTGCCCGT AGGGAGAAAC AGCAAAGGTG GACCCGAAGG 
GAGGCATGTG ACTTTTTTCG TGTGCTGTCC AGCTTTGGTG TCGAGTATGA CCCCGATACT 
CAACTGTATG ACTGGCAGAG GTTCCGTGGT CTGGCAAGAC TGGACAAGAA GACAGATGAG 
TCACTGCTCA AATACTTCCA TGGATTTGTG GCAATGTGCC GCCAGGTTTG CCGCTTACCC 
CCTGCTGCCG GAGACGAGCC ACCAGATCCA TCTCTCTTCA TTGAGCCAAT TTCAGAAGAG 
CGAGCATCTC GCGCCCTGTT TCGCTTGGAT CTTCTTCGTA GGGTGCGTGA GCAAGTACTT 
TGTCATCCTT TGCTTATTCC TCGTTTGTCA CTGTGCCGGC CAGACCCAGA ACTCCCAGAA 
TGGTGGGAGA GTGGACGTCA TGACAATGAG CTTCTTCAGG GAGCAGCACG TTATGGTCTG 
AGCCGTACAG ACCTCACTAT ACTGCAGGAC CATGCATTTT CTTTTTTGCG GTGTCAGATC 
AGTTATTTCC AGTCAAGGGG AATATCTGGC CATTCTCGCC CTTCTACTCC AACTACTGCC 
CCTTCAGTGC CTACTGAACG TCAGCCTTCT CATCTAGCCT CCCTTTCCTC ATCTGTCAGC 
TGTTCTCCTG CTCCACGCAG ATCCTCTTCC TGCTCATCAT CATCTCACTC ATCAAACTCT 
GAGGATTCTT CAGATGAAAG CAATGGTGTA AAAGTAAAAC CTAATGCAGG TCTTTCGCAC 
GCTCGACTGT ATGATGAGGA GAGCCGGCTT TCTCTTACTG CCTCCCCTGC AGATCTCACT 
ACAGAGGACA GTATTCAGAC AGCGCTGGAG ACCCCGCACA GTACAGACTG GCCAAAGGAC 
CGTGTCCTTA TCTGCAGAAT TGAGCAAGTA TGCAGTGCCG TTCTTACTGG CAAATGGTCC 
TCACCACGAC GACTGTCAGA TCCACCAAGT GACAGCCCTG ATAGCTTGCC TCCAACCCCA 
GAGCAGCAGA GTCCAGCCCA CTTCACACAG ATAAGACCTG CACCGGACCC CAAAGAGTTT 
ACCATTCAAA TAAAAAGTGA AGAAGGCCTG AAGATTAAAT TTCAGAAGCA CAAATTTATG 
GGTAATGGAG CTCTTGATTC TGCACCTCAT ACCCAGAAGA AAAAGAGCAA GAAAAAGATG 
GATCTTGACA TGGACACACG AATTCCCGTT GTAAACCAGA AGGATGGGAC CTTGTTGTTA 
GGGGAAGAAG CCCCTTTACG CTGCCAGCTG CCCGAGTGGT TACACAGAAA TCCTGACTTC 
ATGGTTGACC CACGCTTTAT TGCGTTTATG GAAGACCGCA GGAAAAGAAA AAAACATCGA 
AGCAAAAAGT CCAAATCCTC CTCCCAGATT GGAAACGTAC AGAATCCCCA CTCCCACAAT 
GGAAAGAAGT CTTCCCACAC AGAAAACTCT TTCGAGACGC AACACCTTCC TACTGCAGCC 
AAAAAAAGGA AGAAACGCCC TCCTTCAAAA ATAATAATGC CATCTGCACT ACATGGAGCT 
GGTGATGTTA CACATTCCTT CTCAGACCTT CAACCTGACC ATTCTGGTCA CCCCATGCGG 
TCAGTGTATG GACCATCAGA TGGAGGGCTG TCCCATCAAG CAGGACTCAG CGGGTTGGGA 
TTTACCCGCT TTCATTCATC GCAGACATCT TCAGTGGGCA GAATCCATGG CACTACCTTT 
GATGAGTTAC AAAGTTTTGA GCCCCCACAA CAACATTTTT CTCTGCTTGA TGATCCTATG 
ATGCCAGCAA ATTCCTCCTC CAGCGGTGAT GAACTGGACA GACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751942.1
CDS	200..7207
Translation	MADPIMDLFDDPNLFGLDSLTGDSFGRDGPDTIDDALGLGNVLGPLEPITDRVGLPGADVGNNEVKQQTESQVLPDNPALMSATEIMQPIQLPTNQETLNQGNPFMGASNAGAPKIMILKAPAGMTVGACPVTQIQTLTPHQAANGGKVAFAKVLTGTPLRPGVSIVSGNTVLAKMPSTGVAGQVGAVRPVRQLLLQPVRASAAPGSSESNTGIKPAITLTSAPQQGDPKRITLVLQQPSQVGATAQQGQRHVVLGGLPGKIVLQGNQLAALTQAKTPQGQPAKVVTIQLQVQQQPGAAGQTPQKFQIVQQAPGGVAIAPSGQHTHMLNQQGVQRLSVPLKVVLQPQAGSSQGTAQGLSVVKVLNASEVANLTASQTVVKTSTGGGESRKLDSQKKQEKANRIVAEAIARARARGEQNIPRVLNEDELPSVNPEDDDGSRRKRKKKKGETSDRSKDEKPKKVKGSGSLRSRSKGKPSTITPIVGKKRKRNPSSDNSDAEIMASQASPAEDEDSVQKRRSNRQVKRKKYTEDLDIKITDDEDDEEVDVTGPVKTEPVLLPEPMPLPDSEAVPSMQFFVENPSEEDAAIVDKILSMRLAKKELPTGEYVEVEEYFVKYKNYSYLHCEWATIEQLERDKRIHQKLKRFKTKMTQMQHFLQEDEESFNPDYVEVDRILDESHSTDKDNGEPVVYYLVKWCSLPYEDSTWELKEDVDDGKIEEFKRIEARQPNLKRVARPAATSWKKLELSREYQNGNQLREYQLEGVNWLLFNWYNRQNCILADEMGLGKTIQSITFLQEVYNVGIRGPFLVIAPLSTITNWEREFGSWTQMNTIVYHGSLASRQMIQQYEMYCKDSKGRLIPGAYKFDALITTFEMVLSDCPELREIEWRCVIIDEAHRLKNRNCKLLDSLKHMDLEHKVLLTGTPLQNTVEELFSLLHFLEPTQFSSEAEFLKDFGDLKTEEQVQKLQAILKPMMLRRLKEDVEKNLAPKQETIIEVELTNIQKKYYRAILEKNFSFLTKGASQSNTPNLLNTMMELRKCCNHPYLITGAEEKIISEFREATPVVPPDFHVQAMVRSSGKLVLIDKLLPKLRAGGHKVLIFSQMVRCLDILEDYLIQRRYLYERIDGRVRGNMRQAAIDRFSRPDSDRFVFLLCTRAGGLGINLTAADTCIIFDSDWNPQNDLQAQARCHRIGQSKAVKIYRLITRNSYEREMFDKASLKLGLDKAVLQSMSGRDNHLSGPIQQFTKKEIEDLLRKGAYAAIMDEDDEGSKFCEEDIDQILLRRTTTITIESEGKGSTFSKASFVASENRTDISLDDPNFWQKWAKKADLDLDLLSSKNTLVIDTPRIRKQTRHFTNKDDDMVEFSDLESDDDDRPKARRQDRKHGYGRTDCFRVEKHLLVYGWGRWRDILTHGRFKRGMNERDVEKICRAILVYCLLHYRGDENIKSFIWDLITPAENGKTKQLQNHSGLSIPVPRGRKGKKVKSQSSFDIHKADWIHKYNPDTLFQDEGYKKHLKHQCNKVLLRVRMLYFLRQEVIGNQAMKVLSGVEARQIDIWFPQVDQVEVPSRWWDREADKSLLIGVFKHGYEKYNTMRADPALCFLEKVGGPDEQAIAAEHHAQDFSELPDGGDFDRDIEDPEYKPLHAQKDPEDEIDSLMMDEEISVVDGEEATAMPSGMLWPPGSALTARLRRLITAYQRSYKREQLKLEAEERGDKRRKRCEAAFKLKEIARREKQQRWTRREACDFFRVLSSFGVEYDPDTQLYDWQRFRGLARLDKKTDESLLKYFHGFVAMCRQVCRLPPAAGDEPPDPSLFIEPISEERASRALFRLDLLRRVREQVLCHPLLIPRLSLCRPDPELPEWWESGRHDNELLQGAARYGLSRTDLTILQDHAFSFLRCQISYFQSRGISGHSRPSTPTTAPSVPTERQPSHLASLSSSVSCSPAPRRSSSCSSSSHSSNSEDSSDESNGVKVKPNAGLSHARLYDEESRLSLTASPADLTTEDSIQTALETPHSTDWPKDRVLICRIEQVCSAVLTGKWSSPRRLSDPPSDSPDSLPPTPEQQSPAHFTQIRPAPDPKEFTIQIKSEEGLKIKFQKHKFMGNGALDSAPHTQKKKSKKKMDLDMDTRIPVVNQKDGTLLLGEEAPLRCQLPEWLHRNPDFMVDPRFIAFMEDRRKRKKHRSKKSKSSSQIGNVQNPHSHNGKKSSHTENSFETQHLPTAAKKRKKRPPSKIIMPSALHGAGDVTHSFSDLQPDHSGHPMRSVYGPSDGGLSHQAGLSGLGFTRFHSSQTSSVGRIHGTTFDELQSFEPPQQHFSLLDDPMMPANSSSSGDELDRP

Target ORF information:

RefSeq Version	XM_031896082.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis chromodomain helicase DNA binding protein 8 (chd8), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031896082.1

ORF Insert Sequence:

ATGGCTGACC CTATCATGGA CCTCTTTGAT GATCCAAACC TATTTGGCCT TGACTCTCTC 
ACTGGAGATT CCTTTGGACG GGATGGGCCA GACACTATAG ATGATGCTCT AGGCCTGGGT 
AATGTCTTGG GACCTTTGGA GCCGATAACA GACAGAGTTG GCCTGCCTGG TGCTGATGTT 
GGCAATAATG AAGTTAAACA ACAAACAGAG TCACAGGTTC TCCCGGACAA TCCAGCTCTT 
ATGTCAGCTA CTGAGATCAT GCAGCCCATT CAACTTCCCA CAAACCAGGA AACACTGAAC 
CAAGGAAATC CCTTTATGGG TGCCTCAAAT GCTGGAGCCC CAAAAATTAT GATTCTGAAA 
GCTCCTGCTG GAATGACAGT TGGTGCATGT CCTGTAACTC AGATACAAAC TCTGACACCT 
CACCAAGCTG CTAATGGTGG CAAGGTGGCA TTTGCTAAGG TGCTTACAGG AACCCCATTG 
CGTCCTGGGG TCTCAATTGT ATCTGGAAAC ACTGTCCTTG CCAAAATGCC AAGCACTGGA 
GTTGCAGGGC AGGTAGGAGC AGTGAGGCCT GTCCGTCAGC TACTTCTTCA ACCAGTGCGT 
GCCTCCGCAG CCCCAGGAAG CTCCGAGAGT AATACAGGGA TCAAGCCTGC AATCACACTA 
ACGTCAGCAC CTCAACAGGG GGACCCTAAA AGAATTACTC TCGTCCTACA GCAGCCTTCC 
CAAGTTGGTG CAACTGCACA ACAAGGGCAG CGTCATGTTG TGCTGGGTGG GCTTCCAGGA 
AAGATTGTCC TTCAGGGAAA CCAACTGGCA GCTCTCACAC AAGCTAAAAC CCCTCAAGGT 
CAGCCAGCTA AAGTAGTGAC TATCCAGCTG CAAGTCCAGC AGCAGCCTGG GGCTGCAGGT 
CAGACTCCTC AAAAGTTTCA GATTGTACAA CAAGCCCCAG GAGGTGTGGC AATTGCTCCC 
AGTGGGCAAC ATACACACAT GCTTAATCAA CAAGGAGTTC AGAGGCTTTC TGTTCCACTC 
AAAGTTGTTC TGCAACCACA GGCTGGTTCC TCTCAGGGTA CTGCGCAGGG CCTGTCAGTT 
GTGAAAGTTC TAAATGCAAG TGAAGTTGCC AATTTGACTG CAAGTCAGAC TGTGGTTAAA 
ACATCCACTG GAGGTGGTGA ATCTCGTAAG CTGGACAGCC AGAAGAAGCA AGAGAAAGCT 
AACCGTATTG TAGCTGAAGC TATTGCCAGG GCCCGAGCCA GAGGTGAACA GAATATTCCT 
CGGGTCCTGA ATGAGGATGA GCTTCCCAGT GTGAATCCTG AAGATGATGA TGGAAGCAGG 
CGGAAAAGGA AGAAGAAGAA AGGAGAGACT AGTGATAGGT CCAAGGATGA GAAACCTAAG 
AAGGTCAAAG GTAGTGGAAG CTTAAGGTCT AGGAGCAAAG GCAAACCAAG TACTATCACT 
CCTATTGTGG GTAAGAAAAG GAAGAGAAAC CCATCTTCCG ATAATTCTGA TGCTGAGATC 
ATGGCATCAC AGGCTTCTCC TGCAGAGGAT GAAGACAGTG TCCAGAAGAG ACGTTCAAAT 
CGCCAGGTGA AGCGCAAGAA GTATACAGAG GATTTGGATA TTAAAATAAC AGATGATGAA 
GATGATGAGG AGGTAGATGT GACGGGACCT GTGAAAACAG AACCTGTGCT GCTTCCTGAG 
CCCATGCCTT TACCTGACAG TGAGGCAGTC CCTTCAATGC AGTTCTTCGT GGAGAACCCA 
AGTGAAGAAG ATGCTGCTAT TGTTGACAAA ATACTCTCTA TGCGCCTAGC TAAAAAAGAG 
CTGCCCACTG GAGAGTATGT TGAAGTTGAA GAGTACTTTG TCAAATATAA AAATTATTCC 
TACCTACACT GTGAATGGGC CACAATTGAG CAACTGGAAA GGGACAAGCG TATTCATCAG 
AAACTGAAAA GATTTAAGAC AAAGATGACC CAGATGCAGC ATTTCTTGCA AGAAGATGAA 
GAATCTTTCA ATCCAGACTA TGTAGAGGTA GATCGAATCC TAGACGAATC TCACAGCACT 
GACAAGGACA ATGGTGAACC GGTGGTGTAT TACCTAGTAA AATGGTGCTC TTTACCTTAT 
GAAGATAGCA CATGGGAGCT GAAAGAAGAT GTTGATGATG GAAAGATAGA GGAATTCAAA 
CGAATAGAGG CGAGGCAGCC AAATCTCAAA CGCGTGGCCC GCCCAGCTGC TACATCATGG 
AAGAAGCTGG AATTGTCTCG GGAGTATCAG AATGGCAACC AGTTGAGAGA ATACCAGCTG 
GAAGGTGTTA ATTGGCTTCT CTTTAACTGG TACAATAGGC AAAACTGCAT TTTGGCAGAT 
GAAATGGGTT TGGGGAAGAC CATCCAATCT ATCACATTCT TACAAGAAGT ATATAATGTG 
GGAATACGGG GACCTTTTTT AGTAATTGCT CCACTTTCCA CCATCACGAA CTGGGAGAGA 
GAGTTTGGCA GTTGGACTCA AATGAATACT ATTGTGTATC ATGGCAGCCT TGCTAGTCGC 
CAAATGATCC AGCAATATGA GATGTACTGT AAGGACTCCA AGGGTCGTCT TATTCCTGGT 
GCGTACAAGT TTGATGCTCT TATTACAACT TTTGAAATGG TGCTGTCTGA TTGCCCAGAG 
CTGCGTGAAA TTGAGTGGCG CTGTGTCATA ATTGATGAGG CTCATCGGCT CAAAAACAGA 
AACTGCAAAC TACTGGACAG CCTAAAGCAC ATGGACTTGG AGCACAAAGT CCTTTTAACA 
GGCACTCCAT TACAGAACAC CGTGGAAGAG TTGTTCAGTC TTTTGCATTT CCTGGAGCCA 
ACACAATTTT CCTCTGAAGC AGAGTTTCTT AAGGACTTTG GAGACCTGAA GACAGAGGAA 
CAGGTGCAGA AATTACAGGC CATCTTAAAA CCTATGATGT TGAGGCGATT AAAGGAAGAT 
GTTGAGAAAA ACCTGGCCCC GAAACAAGAA ACTATAATTG AGGTTGAGCT TACTAACATC 
CAGAAGAAGT ATTACCGAGC AATACTGGAG AAGAATTTTT CTTTTCTGAC CAAAGGAGCT 
AGTCAATCCA ACACACCCAA CTTGCTCAAT ACCATGATGG AACTACGCAA ATGCTGCAAC 
CACCCATACC TCATCACTGG AGCAGAAGAA AAGATCATAT CTGAGTTCCG AGAAGCAACT 
CCAGTAGTTC CCCCTGACTT TCACGTCCAA GCAATGGTAC GCTCCTCTGG CAAACTGGTG 
CTAATAGATA AGCTGCTCCC TAAACTAAGG GCTGGAGGCC ACAAAGTTCT CATCTTTTCT 
CAGATGGTAC GGTGCCTGGA CATCCTGGAA GACTATCTCA TTCAGAGAAG GTACCTTTAT 
GAGCGTATTG ATGGACGTGT CCGTGGCAAC ATGCGTCAGG CAGCCATTGA TAGATTCAGC 
CGCCCAGATT CTGACCGATT TGTGTTTCTA CTCTGTACAC GAGCTGGTGG GCTTGGAATT 
AACTTGACAG CTGCTGATAC TTGTATTATA TTTGACTCGG ACTGGAACCC TCAAAATGAC 
CTTCAGGCTC AAGCTCGATG TCATCGTATT GGGCAAAGCA AAGCCGTTAA AATTTACAGA 
CTCATCACTC GCAACTCCTA TGAGAGAGAG ATGTTTGATA AGGCCAGTCT CAAACTAGGA 
CTAGACAAAG CTGTATTGCA GTCTATGAGT GGTCGGGATA ATCACCTTAG TGGGCCTATT 
CAGCAATTCA CAAAGAAAGA AATTGAAGAC TTGCTAAGGA AAGGAGCTTA TGCTGCAATC 
ATGGATGAAG ATGACGAAGG CTCTAAATTC TGTGAAGAAG ATATTGACCA AATTTTACTG 
AGGCGTACCA CGACTATTAC CATAGAGTCA GAGGGAAAGG GCTCAACCTT CTCCAAGGCA 
AGTTTTGTTG CTTCTGAGAA CAGGACTGAT ATATCCCTAG ATGATCCTAA TTTCTGGCAA 
AAGTGGGCCA AGAAAGCAGA CTTAGACTTG GATCTTCTTA GCAGCAAGAA TACACTGGTT 
ATTGATACCC CACGTATCAG GAAGCAAACA CGGCACTTTA CTAACAAAGA TGATGACATG 
GTTGAGTTCT CAGATTTGGA GAGTGATGAT GATGATCGCC CCAAAGCTCG GCGTCAAGAC 
CGCAAGCATG GATATGGACG TACAGATTGC TTCCGTGTGG AGAAGCATTT GCTTGTTTAT 
GGATGGGGTC GCTGGCGAGA TATCCTTACT CATGGTCGTT TCAAGCGTGG TATGAATGAG 
CGTGACGTGG AGAAAATATG TAGAGCCATA CTGGTTTATT GTCTTTTGCA CTACCGGGGA 
GATGAAAACA TAAAGAGCTT TATCTGGGAT CTAATTACAC CAGCTGAGAA TGGCAAAACT 
AAGCAGCTAC AGAACCACTC TGGATTGTCT ATTCCTGTCC CCCGTGGTCG TAAGGGGAAA 
AAAGTGAAAT CCCAAAGCAG CTTTGACATA CATAAAGCCG ATTGGATACA TAAGTACAAC 
CCAGATACCT TGTTCCAAGA TGAAGGGTAC AAGAAACATC TCAAGCATCA ATGTAACAAG 
GTGCTGCTTC GCGTTCGTAT GTTATATTTT CTTCGTCAGG AAGTTATTGG AAACCAGGCA 
ATGAAAGTTT TAAGTGGTGT AGAGGCCAGG CAAATTGACA TCTGGTTCCC ACAGGTGGAC 
CAGGTTGAAG TTCCCTCACG CTGGTGGGAC AGAGAAGCTG ATAAATCTCT TCTTATCGGG 
GTCTTTAAAC ATGGTTATGA GAAGTATAAC ACCATGCGAG CTGACCCTGC ACTCTGTTTT 
TTGGAGAAGG TGGGGGGACC AGATGAACAG GCTATTGCAG CTGAGCACCA TGCTCAAGAC 
TTTTCTGAAC TCCCTGATGG TGGGGACTTT GATCGAGACA TAGAAGATCC AGAATACAAG 
CCACTTCATG CACAGAAAGA TCCTGAGGAT GAGATCGACT CTTTAATGAT GGATGAAGAA 
ATCTCTGTTG TTGACGGTGA AGAAGCAACA GCAATGCCCA GTGGTATGTT ATGGCCACCT 
GGCTCTGCTC TCACAGCTCG TCTACGGCGT CTAATTACTG CCTACCAACG CAGCTACAAA 
AGAGAACAAC TAAAATTGGA GGCTGAAGAG CGTGGGGACA AACGTAGAAA ACGATGTGAG 
GCAGCCTTTA AACTGAAAGA AATTGCCCGT AGGGAGAAAC AGCAAAGGTG GACCCGAAGG 
GAGGCATGTG ACTTTTTTCG TGTGCTGTCC AGCTTTGGTG TCGAGTATGA CCCCGATACT 
CAACTGTATG ACTGGCAGAG GTTCCGTGGT CTGGCAAGAC TGGACAAGAA GACAGATGAG 
TCACTGCTCA AATACTTCCA TGGATTTGTG GCAATGTGCC GCCAGGTTTG CCGCTTACCC 
CCTGCTGCCG GAGACGAGCC ACCAGATCCA TCTCTCTTCA TTGAGCCAAT TTCAGAAGAG 
CGAGCATCTC GCGCCCTGTT TCGCTTGGAT CTTCTTCGTA GGGTGCGTGA GCAAGTACTT 
TGTCATCCTT TGCTTATTCC TCGTTTGTCA CTGTGCCGGC CAGACCCAGA ACTCCCAGAA 
TGGTGGGAGA GTGGACGTCA TGACAATGAG CTTCTTCAGG GAGCAGCACG TTATGGTCTG 
AGCCGTACAG ACCTCACTAT ACTGCAGGAC CATGCATTTT CTTTTTTGCG GTGTCAGATC 
AGTTATTTCC AGTCAAGGGG AATATCTGGC CATTCTCGCC CTTCTACTCC AACTACTGCC 
CCTTCAGTGC CTACTGAACG TCAGCCTTCT CATCTAGCCT CCCTTTCCTC ATCTGTCAGC 
TGTTCTCCTG CTCCACGCAG ATCCTCTTCC TGCTCATCAT CATCTCACTC ATCAAACTCT 
GAGGATTCTT CAGATGAAAG CAATGGTGTA AAAGTAAAAC CTAATGCAGG TCTTTCGCAC 
GCTCGACTGT ATGATGAGGA GAGCCGGCTT TCTCTTACTG CCTCCCCTGC AGATCTCACT 
ACAGAGGACA GTATTCAGAC AGCGCTGGAG ACCCCGCACA GTACAGACTG GCCAAAGGAC 
CGTGTCCTTA TCTGCAGAAT TGAGCAAGTA TGCAGTGCCG TTCTTACTGG CAAATGGTCC 
TCACCACGAC GACTGTCAGA TCCACCAAGT GACAGCCCTG ATAGCTTGCC TCCAACCCCA 
GAGCAGCAGA GTCCAGCCCA CTTCACACAG ATAAGACCTG CACCGGACCC CAAAGAGTTT 
ACCATTCAAA TAAAAAGTGA AGAAGGCCTG AAGATTAAAT TTCAGAAGCA CAAATTTATG 
GGTAATGGAG CTCTTGATTC TGCACCTCAT ACCCAGAAGA AAAAGAGCAA GAAAAAGATG 
GATCTTGACA TGGACACACG AATTCCCGTT GTAAACCAGA AGGATGGGAC CTTGTTGTTA 
GGGGAAGAAG CCCCTTTACG CTGCCAGCTG CCCGAGTGGT TACACAGAAA TCCTGACTTC 
ATGGTTGACC CACGCTTTAT TGCGTTTATG GAAGACCGCA GGAAAAGAAA AAAACATCGA 
AGCAAAAAGT CCAAATCCTC CTCCCAGATT GGAAACGTAC AGAATCCCCA CTCCCACAAT 
GGAAAGAAGT CTTCCCACAC AGAAAACTCT TTCGAGACGC AACACCTTCC TACTGCAGCC 
AAAAAAAGGA AGAAACGCCC TCCTTCAAAA ATAATAATGC CATCTGCACT ACATGGAGCT 
GGTGATGTTA CACATTCCTT CTCAGACCTT CAACCTGACC ATTCTGGTCA CCCCATGCGG 
TCAGTGTATG GACCATCAGA TGGAGGGCTG TCCCATCAAG CAGGACTCAG CGGGTTGGGA 
TTTACCCGCT TTCATTCATC GCAGACATCT TCAGTGGGCA GAATCCATGG CACTACCTTT 
GATGAGTTAC AAAGTTTTGA GCCCCCACAA CAACATTTTT CTCTGCTTGA TGATCCTATG 
ATGCCAGCAA ATTCCTCCTC CAGCGGTGAT GAACTGGACA GACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chd8 ( XM_012957182.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012812636.1 Xenopus tropicalis chromodomain helicase DNA binding protein 8 (chd8), transcript variant X3, mRNA.

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CloneID	OXa32500
Clone ID Related Accession (Same CDS sequence)	XM_012957172.3 , XM_012957178.3 , XM_031896082.1 , XM_012957178.2 , XM_012957172.2 , XM_012957182.2 , XM_012957182.3
Accession Version	XM_012957182.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7008bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	chromodomain-helicase-DNA-binding protein 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957182.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACC CTATCATGGA CCTCTTTGAT GATCCAAACC TATTTGGCCT TGACTCTCTC 
ACTGGAGATT CCTTTGGACG GGATGGGCCA GACACTATAG ATGATGCTCT AGGCCTGGGT 
AATGTCTTGG GACCTTTGGA GCCGATAACA GACAGAGTTG GCCTGCCTGG TGCTGATGTT 
GGCAATAATG AAGTTAAACA ACAAACAGAG TCACAGGTTC TCCCGGACAA TCCAGCTCTT 
ATGTCAGCTA CTGAGATCAT GCAGCCCATT CAACTTCCCA CAAACCAGGA AACACTGAAC 
CAAGGAAATC CCTTTATGGG TGCCTCAAAT GCTGGAGCCC CAAAAATTAT GATTCTGAAA 
GCTCCTGCTG GAATGACAGT TGGTGCATGT CCTGTAACTC AGATACAAAC TCTGACACCT 
CACCAAGCTG CTAATGGTGG CAAGGTGGCA TTTGCTAAGG TGCTTACAGG AACCCCATTG 
CGTCCTGGGG TCTCAATTGT ATCTGGAAAC ACTGTCCTTG CCAAAATGCC AAGCACTGGA 
GTTGCAGGGC AGGTAGGAGC AGTGAGGCCT GTCCGTCAGC TACTTCTTCA ACCAGTGCGT 
GCCTCCGCAG CCCCAGGAAG CTCCGAGAGT AATACAGGGA TCAAGCCTGC AATCACACTA 
ACGTCAGCAC CTCAACAGGG GGACCCTAAA AGAATTACTC TCGTCCTACA GCAGCCTTCC 
CAAGTTGGTG CAACTGCACA ACAAGGGCAG CGTCATGTTG TGCTGGGTGG GCTTCCAGGA 
AAGATTGTCC TTCAGGGAAA CCAACTGGCA GCTCTCACAC AAGCTAAAAC CCCTCAAGGT 
CAGCCAGCTA AAGTAGTGAC TATCCAGCTG CAAGTCCAGC AGCAGCCTGG GGCTGCAGGT 
CAGACTCCTC AAAAGTTTCA GATTGTACAA CAAGCCCCAG GAGGTGTGGC AATTGCTCCC 
AGTGGGCAAC ATACACACAT GCTTAATCAA CAAGGAGTTC AGAGGCTTTC TGTTCCACTC 
AAAGTTGTTC TGCAACCACA GGCTGGTTCC TCTCAGGGTA CTGCGCAGGG CCTGTCAGTT 
GTGAAAGTTC TAAATGCAAG TGAAGTTGCC AATTTGACTG CAAGTCAGAC TGTGGTTAAA 
ACATCCACTG GAGGTGGTGA ATCTCGTAAG CTGGACAGCC AGAAGAAGCA AGAGAAAGCT 
AACCGTATTG TAGCTGAAGC TATTGCCAGG GCCCGAGCCA GAGGTGAACA GAATATTCCT 
CGGGTCCTGA ATGAGGATGA GCTTCCCAGT GTGAATCCTG AAGATGATGA TGGAAGCAGG 
CGGAAAAGGA AGAAGAAGAA AGGAGAGACT AGTGATAGGT CCAAGGATGA GAAACCTAAG 
AAGGTCAAAG GTAGTGGAAG CTTAAGGTCT AGGAGCAAAG GCAAACCAAG TACTATCACT 
CCTATTGTGG GTAAGAAAAG GAAGAGAAAC CCATCTTCCG ATAATTCTGA TGCTGAGATC 
ATGGCATCAC AGGCTTCTCC TGCAGAGGAT GAAGACAGTG TCCAGAAGAG ACGTTCAAAT 
CGCCAGGTGA AGCGCAAGAA GTATACAGAG GATTTGGATA TTAAAATAAC AGATGATGAA 
GATGATGAGG AGGTAGATGT GACGGGACCT GTGAAAACAG AACCTGTGCT GCTTCCTGAG 
CCCATGCCTT TACCTGACAG TGAGGCAGTC CCTTCAATGC AGTTCTTCGT GGAGAACCCA 
AGTGAAGAAG ATGCTGCTAT TGTTGACAAA ATACTCTCTA TGCGCCTAGC TAAAAAAGAG 
CTGCCCACTG GAGAGTATGT TGAAGTTGAA GAGTACTTTG TCAAATATAA AAATTATTCC 
TACCTACACT GTGAATGGGC CACAATTGAG CAACTGGAAA GGGACAAGCG TATTCATCAG 
AAACTGAAAA GATTTAAGAC AAAGATGACC CAGATGCAGC ATTTCTTGCA AGAAGATGAA 
GAATCTTTCA ATCCAGACTA TGTAGAGGTA GATCGAATCC TAGACGAATC TCACAGCACT 
GACAAGGACA ATGGTGAACC GGTGGTGTAT TACCTAGTAA AATGGTGCTC TTTACCTTAT 
GAAGATAGCA CATGGGAGCT GAAAGAAGAT GTTGATGATG GAAAGATAGA GGAATTCAAA 
CGAATAGAGG CGAGGCAGCC AAATCTCAAA CGCGTGGCCC GCCCAGCTGC TACATCATGG 
AAGAAGCTGG AATTGTCTCG GGAGTATCAG AATGGCAACC AGTTGAGAGA ATACCAGCTG 
GAAGGTGTTA ATTGGCTTCT CTTTAACTGG TACAATAGGC AAAACTGCAT TTTGGCAGAT 
GAAATGGGTT TGGGGAAGAC CATCCAATCT ATCACATTCT TACAAGAAGT ATATAATGTG 
GGAATACGGG GACCTTTTTT AGTAATTGCT CCACTTTCCA CCATCACGAA CTGGGAGAGA 
GAGTTTGGCA GTTGGACTCA AATGAATACT ATTGTGTATC ATGGCAGCCT TGCTAGTCGC 
CAAATGATCC AGCAATATGA GATGTACTGT AAGGACTCCA AGGGTCGTCT TATTCCTGGT 
GCGTACAAGT TTGATGCTCT TATTACAACT TTTGAAATGG TGCTGTCTGA TTGCCCAGAG 
CTGCGTGAAA TTGAGTGGCG CTGTGTCATA ATTGATGAGG CTCATCGGCT CAAAAACAGA 
AACTGCAAAC TACTGGACAG CCTAAAGCAC ATGGACTTGG AGCACAAAGT CCTTTTAACA 
GGCACTCCAT TACAGAACAC CGTGGAAGAG TTGTTCAGTC TTTTGCATTT CCTGGAGCCA 
ACACAATTTT CCTCTGAAGC AGAGTTTCTT AAGGACTTTG GAGACCTGAA GACAGAGGAA 
CAGGTGCAGA AATTACAGGC CATCTTAAAA CCTATGATGT TGAGGCGATT AAAGGAAGAT 
GTTGAGAAAA ACCTGGCCCC GAAACAAGAA ACTATAATTG AGGTTGAGCT TACTAACATC 
CAGAAGAAGT ATTACCGAGC AATACTGGAG AAGAATTTTT CTTTTCTGAC CAAAGGAGCT 
AGTCAATCCA ACACACCCAA CTTGCTCAAT ACCATGATGG AACTACGCAA ATGCTGCAAC 
CACCCATACC TCATCACTGG AGCAGAAGAA AAGATCATAT CTGAGTTCCG AGAAGCAACT 
CCAGTAGTTC CCCCTGACTT TCACGTCCAA GCAATGGTAC GCTCCTCTGG CAAACTGGTG 
CTAATAGATA AGCTGCTCCC TAAACTAAGG GCTGGAGGCC ACAAAGTTCT CATCTTTTCT 
CAGATGGTAC GGTGCCTGGA CATCCTGGAA GACTATCTCA TTCAGAGAAG GTACCTTTAT 
GAGCGTATTG ATGGACGTGT CCGTGGCAAC ATGCGTCAGG CAGCCATTGA TAGATTCAGC 
CGCCCAGATT CTGACCGATT TGTGTTTCTA CTCTGTACAC GAGCTGGTGG GCTTGGAATT 
AACTTGACAG CTGCTGATAC TTGTATTATA TTTGACTCGG ACTGGAACCC TCAAAATGAC 
CTTCAGGCTC AAGCTCGATG TCATCGTATT GGGCAAAGCA AAGCCGTTAA AATTTACAGA 
CTCATCACTC GCAACTCCTA TGAGAGAGAG ATGTTTGATA AGGCCAGTCT CAAACTAGGA 
CTAGACAAAG CTGTATTGCA GTCTATGAGT GGTCGGGATA ATCACCTTAG TGGGCCTATT 
CAGCAATTCA CAAAGAAAGA AATTGAAGAC TTGCTAAGGA AAGGAGCTTA TGCTGCAATC 
ATGGATGAAG ATGACGAAGG CTCTAAATTC TGTGAAGAAG ATATTGACCA AATTTTACTG 
AGGCGTACCA CGACTATTAC CATAGAGTCA GAGGGAAAGG GCTCAACCTT CTCCAAGGCA 
AGTTTTGTTG CTTCTGAGAA CAGGACTGAT ATATCCCTAG ATGATCCTAA TTTCTGGCAA 
AAGTGGGCCA AGAAAGCAGA CTTAGACTTG GATCTTCTTA GCAGCAAGAA TACACTGGTT 
ATTGATACCC CACGTATCAG GAAGCAAACA CGGCACTTTA CTAACAAAGA TGATGACATG 
GTTGAGTTCT CAGATTTGGA GAGTGATGAT GATGATCGCC CCAAAGCTCG GCGTCAAGAC 
CGCAAGCATG GATATGGACG TACAGATTGC TTCCGTGTGG AGAAGCATTT GCTTGTTTAT 
GGATGGGGTC GCTGGCGAGA TATCCTTACT CATGGTCGTT TCAAGCGTGG TATGAATGAG 
CGTGACGTGG AGAAAATATG TAGAGCCATA CTGGTTTATT GTCTTTTGCA CTACCGGGGA 
GATGAAAACA TAAAGAGCTT TATCTGGGAT CTAATTACAC CAGCTGAGAA TGGCAAAACT 
AAGCAGCTAC AGAACCACTC TGGATTGTCT ATTCCTGTCC CCCGTGGTCG TAAGGGGAAA 
AAAGTGAAAT CCCAAAGCAG CTTTGACATA CATAAAGCCG ATTGGATACA TAAGTACAAC 
CCAGATACCT TGTTCCAAGA TGAAGGGTAC AAGAAACATC TCAAGCATCA ATGTAACAAG 
GTGCTGCTTC GCGTTCGTAT GTTATATTTT CTTCGTCAGG AAGTTATTGG AAACCAGGCA 
ATGAAAGTTT TAAGTGGTGT AGAGGCCAGG CAAATTGACA TCTGGTTCCC ACAGGTGGAC 
CAGGTTGAAG TTCCCTCACG CTGGTGGGAC AGAGAAGCTG ATAAATCTCT TCTTATCGGG 
GTCTTTAAAC ATGGTTATGA GAAGTATAAC ACCATGCGAG CTGACCCTGC ACTCTGTTTT 
TTGGAGAAGG TGGGGGGACC AGATGAACAG GCTATTGCAG CTGAGCACCA TGCTCAAGAC 
TTTTCTGAAC TCCCTGATGG TGGGGACTTT GATCGAGACA TAGAAGATCC AGAATACAAG 
CCACTTCATG CACAGAAAGA TCCTGAGGAT GAGATCGACT CTTTAATGAT GGATGAAGAA 
ATCTCTGTTG TTGACGGTGA AGAAGCAACA GCAATGCCCA GTGGTATGTT ATGGCCACCT 
GGCTCTGCTC TCACAGCTCG TCTACGGCGT CTAATTACTG CCTACCAACG CAGCTACAAA 
AGAGAACAAC TAAAATTGGA GGCTGAAGAG CGTGGGGACA AACGTAGAAA ACGATGTGAG 
GCAGCCTTTA AACTGAAAGA AATTGCCCGT AGGGAGAAAC AGCAAAGGTG GACCCGAAGG 
GAGGCATGTG ACTTTTTTCG TGTGCTGTCC AGCTTTGGTG TCGAGTATGA CCCCGATACT 
CAACTGTATG ACTGGCAGAG GTTCCGTGGT CTGGCAAGAC TGGACAAGAA GACAGATGAG 
TCACTGCTCA AATACTTCCA TGGATTTGTG GCAATGTGCC GCCAGGTTTG CCGCTTACCC 
CCTGCTGCCG GAGACGAGCC ACCAGATCCA TCTCTCTTCA TTGAGCCAAT TTCAGAAGAG 
CGAGCATCTC GCGCCCTGTT TCGCTTGGAT CTTCTTCGTA GGGTGCGTGA GCAAGTACTT 
TGTCATCCTT TGCTTATTCC TCGTTTGTCA CTGTGCCGGC CAGACCCAGA ACTCCCAGAA 
TGGTGGGAGA GTGGACGTCA TGACAATGAG CTTCTTCAGG GAGCAGCACG TTATGGTCTG 
AGCCGTACAG ACCTCACTAT ACTGCAGGAC CATGCATTTT CTTTTTTGCG GTGTCAGATC 
AGTTATTTCC AGTCAAGGGG AATATCTGGC CATTCTCGCC CTTCTACTCC AACTACTGCC 
CCTTCAGTGC CTACTGAACG TCAGCCTTCT CATCTAGCCT CCCTTTCCTC ATCTGTCAGC 
TGTTCTCCTG CTCCACGCAG ATCCTCTTCC TGCTCATCAT CATCTCACTC ATCAAACTCT 
GAGGATTCTT CAGATGAAAG CAATGGTGTA AAAGTAAAAC CTAATGCAGG TCTTTCGCAC 
GCTCGACTGT ATGATGAGGA GAGCCGGCTT TCTCTTACTG CCTCCCCTGC AGATCTCACT 
ACAGAGGACA GTATTCAGAC AGCGCTGGAG ACCCCGCACA GTACAGACTG GCCAAAGGAC 
CGTGTCCTTA TCTGCAGAAT TGAGCAAGTA TGCAGTGCCG TTCTTACTGG CAAATGGTCC 
TCACCACGAC GACTGTCAGA TCCACCAAGT GACAGCCCTG ATAGCTTGCC TCCAACCCCA 
GAGCAGCAGA GTCCAGCCCA CTTCACACAG ATAAGACCTG CACCGGACCC CAAAGAGTTT 
ACCATTCAAA TAAAAAGTGA AGAAGGCCTG AAGATTAAAT TTCAGAAGCA CAAATTTATG 
GGTAATGGAG CTCTTGATTC TGCACCTCAT ACCCAGAAGA AAAAGAGCAA GAAAAAGATG 
GATCTTGACA TGGACACACG AATTCCCGTT GTAAACCAGA AGGATGGGAC CTTGTTGTTA 
GGGGAAGAAG CCCCTTTACG CTGCCAGCTG CCCGAGTGGT TACACAGAAA TCCTGACTTC 
ATGGTTGACC CACGCTTTAT TGCGTTTATG GAAGACCGCA GGAAAAGAAA AAAACATCGA 
AGCAAAAAGT CCAAATCCTC CTCCCAGATT GGAAACGTAC AGAATCCCCA CTCCCACAAT 
GGAAAGAAGT CTTCCCACAC AGAAAACTCT TTCGAGACGC AACACCTTCC TACTGCAGCC 
AAAAAAAGGA AGAAACGCCC TCCTTCAAAA ATAATAATGC CATCTGCACT ACATGGAGCT 
GGTGATGTTA CACATTCCTT CTCAGACCTT CAACCTGACC ATTCTGGTCA CCCCATGCGG 
TCAGTGTATG GACCATCAGA TGGAGGGCTG TCCCATCAAG CAGGACTCAG CGGGTTGGGA 
TTTACCCGCT TTCATTCATC GCAGACATCT TCAGTGGGCA GAATCCATGG CACTACCTTT 
GATGAGTTAC AAAGTTTTGA GCCCCCACAA CAACATTTTT CTCTGCTTGA TGATCCTATG 
ATGCCAGCAA ATTCCTCCTC CAGCGGTGAT GAACTGGACA GACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012812636.1
CDS	237..7244
Translation	MADPIMDLFDDPNLFGLDSLTGDSFGRDGPDTIDDALGLGNVLGPLEPITDRVGLPGADVGNNEVKQQTESQVLPDNPALMSATEIMQPIQLPTNQETLNQGNPFMGASNAGAPKIMILKAPAGMTVGACPVTQIQTLTPHQAANGGKVAFAKVLTGTPLRPGVSIVSGNTVLAKMPSTGVAGQVGAVRPVRQLLLQPVRASAAPGSSESNTGIKPAITLTSAPQQGDPKRITLVLQQPSQVGATAQQGQRHVVLGGLPGKIVLQGNQLAALTQAKTPQGQPAKVVTIQLQVQQQPGAAGQTPQKFQIVQQAPGGVAIAPSGQHTHMLNQQGVQRLSVPLKVVLQPQAGSSQGTAQGLSVVKVLNASEVANLTASQTVVKTSTGGGESRKLDSQKKQEKANRIVAEAIARARARGEQNIPRVLNEDELPSVNPEDDDGSRRKRKKKKGETSDRSKDEKPKKVKGSGSLRSRSKGKPSTITPIVGKKRKRNPSSDNSDAEIMASQASPAEDEDSVQKRRSNRQVKRKKYTEDLDIKITDDEDDEEVDVTGPVKTEPVLLPEPMPLPDSEAVPSMQFFVENPSEEDAAIVDKILSMRLAKKELPTGEYVEVEEYFVKYKNYSYLHCEWATIEQLERDKRIHQKLKRFKTKMTQMQHFLQEDEESFNPDYVEVDRILDESHSTDKDNGEPVVYYLVKWCSLPYEDSTWELKEDVDDGKIEEFKRIEARQPNLKRVARPAATSWKKLELSREYQNGNQLREYQLEGVNWLLFNWYNRQNCILADEMGLGKTIQSITFLQEVYNVGIRGPFLVIAPLSTITNWEREFGSWTQMNTIVYHGSLASRQMIQQYEMYCKDSKGRLIPGAYKFDALITTFEMVLSDCPELREIEWRCVIIDEAHRLKNRNCKLLDSLKHMDLEHKVLLTGTPLQNTVEELFSLLHFLEPTQFSSEAEFLKDFGDLKTEEQVQKLQAILKPMMLRRLKEDVEKNLAPKQETIIEVELTNIQKKYYRAILEKNFSFLTKGASQSNTPNLLNTMMELRKCCNHPYLITGAEEKIISEFREATPVVPPDFHVQAMVRSSGKLVLIDKLLPKLRAGGHKVLIFSQMVRCLDILEDYLIQRRYLYERIDGRVRGNMRQAAIDRFSRPDSDRFVFLLCTRAGGLGINLTAADTCIIFDSDWNPQNDLQAQARCHRIGQSKAVKIYRLITRNSYEREMFDKASLKLGLDKAVLQSMSGRDNHLSGPIQQFTKKEIEDLLRKGAYAAIMDEDDEGSKFCEEDIDQILLRRTTTITIESEGKGSTFSKASFVASENRTDISLDDPNFWQKWAKKADLDLDLLSSKNTLVIDTPRIRKQTRHFTNKDDDMVEFSDLESDDDDRPKARRQDRKHGYGRTDCFRVEKHLLVYGWGRWRDILTHGRFKRGMNERDVEKICRAILVYCLLHYRGDENIKSFIWDLITPAENGKTKQLQNHSGLSIPVPRGRKGKKVKSQSSFDIHKADWIHKYNPDTLFQDEGYKKHLKHQCNKVLLRVRMLYFLRQEVIGNQAMKVLSGVEARQIDIWFPQVDQVEVPSRWWDREADKSLLIGVFKHGYEKYNTMRADPALCFLEKVGGPDEQAIAAEHHAQDFSELPDGGDFDRDIEDPEYKPLHAQKDPEDEIDSLMMDEEISVVDGEEATAMPSGMLWPPGSALTARLRRLITAYQRSYKREQLKLEAEERGDKRRKRCEAAFKLKEIARREKQQRWTRREACDFFRVLSSFGVEYDPDTQLYDWQRFRGLARLDKKTDESLLKYFHGFVAMCRQVCRLPPAAGDEPPDPSLFIEPISEERASRALFRLDLLRRVREQVLCHPLLIPRLSLCRPDPELPEWWESGRHDNELLQGAARYGLSRTDLTILQDHAFSFLRCQISYFQSRGISGHSRPSTPTTAPSVPTERQPSHLASLSSSVSCSPAPRRSSSCSSSSHSSNSEDSSDESNGVKVKPNAGLSHARLYDEESRLSLTASPADLTTEDSIQTALETPHSTDWPKDRVLICRIEQVCSAVLTGKWSSPRRLSDPPSDSPDSLPPTPEQQSPAHFTQIRPAPDPKEFTIQIKSEEGLKIKFQKHKFMGNGALDSAPHTQKKKSKKKMDLDMDTRIPVVNQKDGTLLLGEEAPLRCQLPEWLHRNPDFMVDPRFIAFMEDRRKRKKHRSKKSKSSSQIGNVQNPHSHNGKKSSHTENSFETQHLPTAAKKRKKRPPSKIIMPSALHGAGDVTHSFSDLQPDHSGHPMRSVYGPSDGGLSHQAGLSGLGFTRFHSSQTSSVGRIHGTTFDELQSFEPPQQHFSLLDDPMMPANSSSSGDELDRP

Target ORF information:

RefSeq Version	XM_012957182.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis chromodomain helicase DNA binding protein 8 (chd8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957182.3

ORF Insert Sequence:

ATGGCTGACC CTATCATGGA CCTCTTTGAT GATCCAAACC TATTTGGCCT TGACTCTCTC 
ACTGGAGATT CCTTTGGACG GGATGGGCCA GACACTATAG ATGATGCTCT AGGCCTGGGT 
AATGTCTTGG GACCTTTGGA GCCGATAACA GACAGAGTTG GCCTGCCTGG TGCTGATGTT 
GGCAATAATG AAGTTAAACA ACAAACAGAG TCACAGGTTC TCCCGGACAA TCCAGCTCTT 
ATGTCAGCTA CTGAGATCAT GCAGCCCATT CAACTTCCCA CAAACCAGGA AACACTGAAC 
CAAGGAAATC CCTTTATGGG TGCCTCAAAT GCTGGAGCCC CAAAAATTAT GATTCTGAAA 
GCTCCTGCTG GAATGACAGT TGGTGCATGT CCTGTAACTC AGATACAAAC TCTGACACCT 
CACCAAGCTG CTAATGGTGG CAAGGTGGCA TTTGCTAAGG TGCTTACAGG AACCCCATTG 
CGTCCTGGGG TCTCAATTGT ATCTGGAAAC ACTGTCCTTG CCAAAATGCC AAGCACTGGA 
GTTGCAGGGC AGGTAGGAGC AGTGAGGCCT GTCCGTCAGC TACTTCTTCA ACCAGTGCGT 
GCCTCCGCAG CCCCAGGAAG CTCCGAGAGT AATACAGGGA TCAAGCCTGC AATCACACTA 
ACGTCAGCAC CTCAACAGGG GGACCCTAAA AGAATTACTC TCGTCCTACA GCAGCCTTCC 
CAAGTTGGTG CAACTGCACA ACAAGGGCAG CGTCATGTTG TGCTGGGTGG GCTTCCAGGA 
AAGATTGTCC TTCAGGGAAA CCAACTGGCA GCTCTCACAC AAGCTAAAAC CCCTCAAGGT 
CAGCCAGCTA AAGTAGTGAC TATCCAGCTG CAAGTCCAGC AGCAGCCTGG GGCTGCAGGT 
CAGACTCCTC AAAAGTTTCA GATTGTACAA CAAGCCCCAG GAGGTGTGGC AATTGCTCCC 
AGTGGGCAAC ATACACACAT GCTTAATCAA CAAGGAGTTC AGAGGCTTTC TGTTCCACTC 
AAAGTTGTTC TGCAACCACA GGCTGGTTCC TCTCAGGGTA CTGCGCAGGG CCTGTCAGTT 
GTGAAAGTTC TAAATGCAAG TGAAGTTGCC AATTTGACTG CAAGTCAGAC TGTGGTTAAA 
ACATCCACTG GAGGTGGTGA ATCTCGTAAG CTGGACAGCC AGAAGAAGCA AGAGAAAGCT 
AACCGTATTG TAGCTGAAGC TATTGCCAGG GCCCGAGCCA GAGGTGAACA GAATATTCCT 
CGGGTCCTGA ATGAGGATGA GCTTCCCAGT GTGAATCCTG AAGATGATGA TGGAAGCAGG 
CGGAAAAGGA AGAAGAAGAA AGGAGAGACT AGTGATAGGT CCAAGGATGA GAAACCTAAG 
AAGGTCAAAG GTAGTGGAAG CTTAAGGTCT AGGAGCAAAG GCAAACCAAG TACTATCACT 
CCTATTGTGG GTAAGAAAAG GAAGAGAAAC CCATCTTCCG ATAATTCTGA TGCTGAGATC 
ATGGCATCAC AGGCTTCTCC TGCAGAGGAT GAAGACAGTG TCCAGAAGAG ACGTTCAAAT 
CGCCAGGTGA AGCGCAAGAA GTATACAGAG GATTTGGATA TTAAAATAAC AGATGATGAA 
GATGATGAGG AGGTAGATGT GACGGGACCT GTGAAAACAG AACCTGTGCT GCTTCCTGAG 
CCCATGCCTT TACCTGACAG TGAGGCAGTC CCTTCAATGC AGTTCTTCGT GGAGAACCCA 
AGTGAAGAAG ATGCTGCTAT TGTTGACAAA ATACTCTCTA TGCGCCTAGC TAAAAAAGAG 
CTGCCCACTG GAGAGTATGT TGAAGTTGAA GAGTACTTTG TCAAATATAA AAATTATTCC 
TACCTACACT GTGAATGGGC CACAATTGAG CAACTGGAAA GGGACAAGCG TATTCATCAG 
AAACTGAAAA GATTTAAGAC AAAGATGACC CAGATGCAGC ATTTCTTGCA AGAAGATGAA 
GAATCTTTCA ATCCAGACTA TGTAGAGGTA GATCGAATCC TAGACGAATC TCACAGCACT 
GACAAGGACA ATGGTGAACC GGTGGTGTAT TACCTAGTAA AATGGTGCTC TTTACCTTAT 
GAAGATAGCA CATGGGAGCT GAAAGAAGAT GTTGATGATG GAAAGATAGA GGAATTCAAA 
CGAATAGAGG CGAGGCAGCC AAATCTCAAA CGCGTGGCCC GCCCAGCTGC TACATCATGG 
AAGAAGCTGG AATTGTCTCG GGAGTATCAG AATGGCAACC AGTTGAGAGA ATACCAGCTG 
GAAGGTGTTA ATTGGCTTCT CTTTAACTGG TACAATAGGC AAAACTGCAT TTTGGCAGAT 
GAAATGGGTT TGGGGAAGAC CATCCAATCT ATCACATTCT TACAAGAAGT ATATAATGTG 
GGAATACGGG GACCTTTTTT AGTAATTGCT CCACTTTCCA CCATCACGAA CTGGGAGAGA 
GAGTTTGGCA GTTGGACTCA AATGAATACT ATTGTGTATC ATGGCAGCCT TGCTAGTCGC 
CAAATGATCC AGCAATATGA GATGTACTGT AAGGACTCCA AGGGTCGTCT TATTCCTGGT 
GCGTACAAGT TTGATGCTCT TATTACAACT TTTGAAATGG TGCTGTCTGA TTGCCCAGAG 
CTGCGTGAAA TTGAGTGGCG CTGTGTCATA ATTGATGAGG CTCATCGGCT CAAAAACAGA 
AACTGCAAAC TACTGGACAG CCTAAAGCAC ATGGACTTGG AGCACAAAGT CCTTTTAACA 
GGCACTCCAT TACAGAACAC CGTGGAAGAG TTGTTCAGTC TTTTGCATTT CCTGGAGCCA 
ACACAATTTT CCTCTGAAGC AGAGTTTCTT AAGGACTTTG GAGACCTGAA GACAGAGGAA 
CAGGTGCAGA AATTACAGGC CATCTTAAAA CCTATGATGT TGAGGCGATT AAAGGAAGAT 
GTTGAGAAAA ACCTGGCCCC GAAACAAGAA ACTATAATTG AGGTTGAGCT TACTAACATC 
CAGAAGAAGT ATTACCGAGC AATACTGGAG AAGAATTTTT CTTTTCTGAC CAAAGGAGCT 
AGTCAATCCA ACACACCCAA CTTGCTCAAT ACCATGATGG AACTACGCAA ATGCTGCAAC 
CACCCATACC TCATCACTGG AGCAGAAGAA AAGATCATAT CTGAGTTCCG AGAAGCAACT 
CCAGTAGTTC CCCCTGACTT TCACGTCCAA GCAATGGTAC GCTCCTCTGG CAAACTGGTG 
CTAATAGATA AGCTGCTCCC TAAACTAAGG GCTGGAGGCC ACAAAGTTCT CATCTTTTCT 
CAGATGGTAC GGTGCCTGGA CATCCTGGAA GACTATCTCA TTCAGAGAAG GTACCTTTAT 
GAGCGTATTG ATGGACGTGT CCGTGGCAAC ATGCGTCAGG CAGCCATTGA TAGATTCAGC 
CGCCCAGATT CTGACCGATT TGTGTTTCTA CTCTGTACAC GAGCTGGTGG GCTTGGAATT 
AACTTGACAG CTGCTGATAC TTGTATTATA TTTGACTCGG ACTGGAACCC TCAAAATGAC 
CTTCAGGCTC AAGCTCGATG TCATCGTATT GGGCAAAGCA AAGCCGTTAA AATTTACAGA 
CTCATCACTC GCAACTCCTA TGAGAGAGAG ATGTTTGATA AGGCCAGTCT CAAACTAGGA 
CTAGACAAAG CTGTATTGCA GTCTATGAGT GGTCGGGATA ATCACCTTAG TGGGCCTATT 
CAGCAATTCA CAAAGAAAGA AATTGAAGAC TTGCTAAGGA AAGGAGCTTA TGCTGCAATC 
ATGGATGAAG ATGACGAAGG CTCTAAATTC TGTGAAGAAG ATATTGACCA AATTTTACTG 
AGGCGTACCA CGACTATTAC CATAGAGTCA GAGGGAAAGG GCTCAACCTT CTCCAAGGCA 
AGTTTTGTTG CTTCTGAGAA CAGGACTGAT ATATCCCTAG ATGATCCTAA TTTCTGGCAA 
AAGTGGGCCA AGAAAGCAGA CTTAGACTTG GATCTTCTTA GCAGCAAGAA TACACTGGTT 
ATTGATACCC CACGTATCAG GAAGCAAACA CGGCACTTTA CTAACAAAGA TGATGACATG 
GTTGAGTTCT CAGATTTGGA GAGTGATGAT GATGATCGCC CCAAAGCTCG GCGTCAAGAC 
CGCAAGCATG GATATGGACG TACAGATTGC TTCCGTGTGG AGAAGCATTT GCTTGTTTAT 
GGATGGGGTC GCTGGCGAGA TATCCTTACT CATGGTCGTT TCAAGCGTGG TATGAATGAG 
CGTGACGTGG AGAAAATATG TAGAGCCATA CTGGTTTATT GTCTTTTGCA CTACCGGGGA 
GATGAAAACA TAAAGAGCTT TATCTGGGAT CTAATTACAC CAGCTGAGAA TGGCAAAACT 
AAGCAGCTAC AGAACCACTC TGGATTGTCT ATTCCTGTCC CCCGTGGTCG TAAGGGGAAA 
AAAGTGAAAT CCCAAAGCAG CTTTGACATA CATAAAGCCG ATTGGATACA TAAGTACAAC 
CCAGATACCT TGTTCCAAGA TGAAGGGTAC AAGAAACATC TCAAGCATCA ATGTAACAAG 
GTGCTGCTTC GCGTTCGTAT GTTATATTTT CTTCGTCAGG AAGTTATTGG AAACCAGGCA 
ATGAAAGTTT TAAGTGGTGT AGAGGCCAGG CAAATTGACA TCTGGTTCCC ACAGGTGGAC 
CAGGTTGAAG TTCCCTCACG CTGGTGGGAC AGAGAAGCTG ATAAATCTCT TCTTATCGGG 
GTCTTTAAAC ATGGTTATGA GAAGTATAAC ACCATGCGAG CTGACCCTGC ACTCTGTTTT 
TTGGAGAAGG TGGGGGGACC AGATGAACAG GCTATTGCAG CTGAGCACCA TGCTCAAGAC 
TTTTCTGAAC TCCCTGATGG TGGGGACTTT GATCGAGACA TAGAAGATCC AGAATACAAG 
CCACTTCATG CACAGAAAGA TCCTGAGGAT GAGATCGACT CTTTAATGAT GGATGAAGAA 
ATCTCTGTTG TTGACGGTGA AGAAGCAACA GCAATGCCCA GTGGTATGTT ATGGCCACCT 
GGCTCTGCTC TCACAGCTCG TCTACGGCGT CTAATTACTG CCTACCAACG CAGCTACAAA 
AGAGAACAAC TAAAATTGGA GGCTGAAGAG CGTGGGGACA AACGTAGAAA ACGATGTGAG 
GCAGCCTTTA AACTGAAAGA AATTGCCCGT AGGGAGAAAC AGCAAAGGTG GACCCGAAGG 
GAGGCATGTG ACTTTTTTCG TGTGCTGTCC AGCTTTGGTG TCGAGTATGA CCCCGATACT 
CAACTGTATG ACTGGCAGAG GTTCCGTGGT CTGGCAAGAC TGGACAAGAA GACAGATGAG 
TCACTGCTCA AATACTTCCA TGGATTTGTG GCAATGTGCC GCCAGGTTTG CCGCTTACCC 
CCTGCTGCCG GAGACGAGCC ACCAGATCCA TCTCTCTTCA TTGAGCCAAT TTCAGAAGAG 
CGAGCATCTC GCGCCCTGTT TCGCTTGGAT CTTCTTCGTA GGGTGCGTGA GCAAGTACTT 
TGTCATCCTT TGCTTATTCC TCGTTTGTCA CTGTGCCGGC CAGACCCAGA ACTCCCAGAA 
TGGTGGGAGA GTGGACGTCA TGACAATGAG CTTCTTCAGG GAGCAGCACG TTATGGTCTG 
AGCCGTACAG ACCTCACTAT ACTGCAGGAC CATGCATTTT CTTTTTTGCG GTGTCAGATC 
AGTTATTTCC AGTCAAGGGG AATATCTGGC CATTCTCGCC CTTCTACTCC AACTACTGCC 
CCTTCAGTGC CTACTGAACG TCAGCCTTCT CATCTAGCCT CCCTTTCCTC ATCTGTCAGC 
TGTTCTCCTG CTCCACGCAG ATCCTCTTCC TGCTCATCAT CATCTCACTC ATCAAACTCT 
GAGGATTCTT CAGATGAAAG CAATGGTGTA AAAGTAAAAC CTAATGCAGG TCTTTCGCAC 
GCTCGACTGT ATGATGAGGA GAGCCGGCTT TCTCTTACTG CCTCCCCTGC AGATCTCACT 
ACAGAGGACA GTATTCAGAC AGCGCTGGAG ACCCCGCACA GTACAGACTG GCCAAAGGAC 
CGTGTCCTTA TCTGCAGAAT TGAGCAAGTA TGCAGTGCCG TTCTTACTGG CAAATGGTCC 
TCACCACGAC GACTGTCAGA TCCACCAAGT GACAGCCCTG ATAGCTTGCC TCCAACCCCA 
GAGCAGCAGA GTCCAGCCCA CTTCACACAG ATAAGACCTG CACCGGACCC CAAAGAGTTT 
ACCATTCAAA TAAAAAGTGA AGAAGGCCTG AAGATTAAAT TTCAGAAGCA CAAATTTATG 
GGTAATGGAG CTCTTGATTC TGCACCTCAT ACCCAGAAGA AAAAGAGCAA GAAAAAGATG 
GATCTTGACA TGGACACACG AATTCCCGTT GTAAACCAGA AGGATGGGAC CTTGTTGTTA 
GGGGAAGAAG CCCCTTTACG CTGCCAGCTG CCCGAGTGGT TACACAGAAA TCCTGACTTC 
ATGGTTGACC CACGCTTTAT TGCGTTTATG GAAGACCGCA GGAAAAGAAA AAAACATCGA 
AGCAAAAAGT CCAAATCCTC CTCCCAGATT GGAAACGTAC AGAATCCCCA CTCCCACAAT 
GGAAAGAAGT CTTCCCACAC AGAAAACTCT TTCGAGACGC AACACCTTCC TACTGCAGCC 
AAAAAAAGGA AGAAACGCCC TCCTTCAAAA ATAATAATGC CATCTGCACT ACATGGAGCT 
GGTGATGTTA CACATTCCTT CTCAGACCTT CAACCTGACC ATTCTGGTCA CCCCATGCGG 
TCAGTGTATG GACCATCAGA TGGAGGGCTG TCCCATCAAG CAGGACTCAG CGGGTTGGGA 
TTTACCCGCT TTCATTCATC GCAGACATCT TCAGTGGGCA GAATCCATGG CACTACCTTT 
GATGAGTTAC AAAGTTTTGA GCCCCCACAA CAACATTTTT CTCTGCTTGA TGATCCTATG 
ATGCCAGCAA ATTCCTCCTC CAGCGGTGAT GAACTGGACA GACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chd8 ( XM_012957198.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012812652.1 Xenopus tropicalis chromodomain helicase DNA binding protein 8 (chd8), transcript variant X5, mRNA.

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CloneID	OXa32501
Clone ID Related Accession (Same CDS sequence)	XM_012957198.3 , XM_012957198.2
Accession Version	XM_012957198.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7005bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	chromodomain-helicase-DNA-binding protein 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957198.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACC CTATCATGGA CCTCTTTGAT GATCCAAACC TATTTGGCCT TGACTCTCTC 
ACTGGAGATT CCTTTGGACG GGATGGGCCA GACACTATAG ATGATGCTCT AGGCCTGGGT 
AATGTCTTGG GACCTTTGGA GCCGATAACA GACAGAGTTG GCCTGCCTGG TGCTGATGTT 
GGCAATAATG AAGTTAAACA ACAAACAGAG TCACAGGTTC TCCCGGACAA TCCAGCTCTT 
ATGTCAGCTA CTGAGATCAT GCAGCCCATT CAACTTCCCA CAAACCAGGA AACACTGAAC 
CAAGGAAATC CCTTTATGGG TGCCTCAAAT GCTGGAGCCC CAAAAATTAT GATTCTGAAA 
GCTCCTGCTG GAATGACAGT TGGTGCATGT CCTGTAACTC AGATACAAAC TCTGACACCT 
CACCAAGCTG CTAATGGTGG CAAGGTGGCA TTTGCTAAGG TGCTTACAGG AACCCCATTG 
CGTCCTGGGG TCTCAATTGT ATCTGGAAAC ACTGTCCTTG CCAAAATGCC AAGCACTGGA 
GTTGCAGGGC AGGTAGGAGC AGTGAGGCCT GTCCGTCAGC TACTTCTTCA ACCAGTGCGT 
GCCTCCGCAG CCCCAGGAAG CTCCGAGAGT AATACAGGGA TCAAGCCTGC AATCACACTA 
ACGTCAGCAC CTCAACAGGG GGACCCTAAA AGAATTACTC TCGTCCTACA GCAGCCTTCC 
CAAGTTGGTG CAACTGCACA ACAAGGGCAG CGTCATGTTG TGCTGGGTGG GCTTCCAGGA 
AAGATTGTCC TTCAGGGAAA CCAACTGGCA GCTCTCACAC AAGCTAAAAC CCCTCAAGGT 
CAGCCAGCTA AAGTAGTGAC TATCCAGCTG CAAGTCCAGC AGCAGCCTGG GGCTGCAGGT 
CAGACTCCTC AAAAGTTTCA GATTGTACAA CAAGCCCCAG GAGGTGTGGC AATTGCTCCC 
AGTGGGCAAC ATACACACAT GCTTAATCAA CAAGGAGTTC AGAGGCTTTC TGTTCCACTC 
AAAGTTGTTC TGCAACCACA GGCTGGTTCC TCTCAGGGTA CTGCGCAGGG CCTGTCAGTT 
GTGAAAGTTC TAAATGCAAG TGAAGTTGCC AATTTGACTG CAAGTCAGAC TGTGGTTAAA 
ACATCCACTG GAGGTGGTGA ATCTCGTAAG CTGGACAGCC AGAAGAAGCA AGAGAAAGCT 
AACCGTATTG TAGCTGAAGC TATTGCCAGG GCCCGAGCCA GAGGTGAACA GAATATTCCT 
CGGGTCCTGA ATGAGGATGA GCTTCCCAGT GTGAATCCTG AAGATGATGA TGGAAGCAGG 
CGGAAAAGGA AGAAGAAGAA AGGAGAGACT AGTGATAGGT CCAAGGATGA GAAACCTAAG 
AAGGTCAAAG GTAGTGGAAG CTTAAGGTCT AGGAGCAAAG GCAAACCAAG TACTATCACT 
CCTATTGTGG GTAAGAAAAG GAAGAGAAAC CCATCTTCCG ATAATTCTGA TGCTGAGATC 
ATGGCATCAC AGGCTTCTCC TGCAGAGGAT GAAGACAGTG TCCAGAAGAG ACGTTCAAAT 
CGCCAGGTGA AGCGCAAGAA GTATACAGAG GATTTGGATA TTAAAATAAC AGATGATGAA 
GATGATGAGG AGGTAGATGT GACGGGACCT GTGAAAACAG AACCTGTGCT GCTTCCTGAG 
CCCATGCCTT TACCTGACAG TGAGGCAGTC CCTTCAATGC AGTTCTTCGT GGAGAACCCA 
AGTGAAGAAG ATGCTGCTAT TGTTGACAAA ATACTCTCTA TGCGCCTAGC TAAAAAAGAG 
CTGCCCACTG GAGAGTATGT TGAAGTTGAA GAGTACTTTG TCAAATATAA AAATTATTCC 
TACCTACACT GTGAATGGGC CACAATTGAG CAACTGGAAA GGGACAAGCG TATTCATCAG 
AAACTGAAAA GATTTAAGAC AAAGATGACC CAGATGCAGC ATTTCTTGCA AGAAGATGAA 
GAATCTTTCA ATCCAGACTA TGTAGAGGTA GATCGAATCC TAGACGAATC TCACAGCACT 
GACAAGGACA ATGGTGAACC GGTGGTGTAT TACCTAGTAA AATGGTGCTC TTTACCTTAT 
GAAGATAGCA CATGGGAGCT GAAAGAAGAT GTTGATGATG GAAAGATAGA GGAATTCAAA 
CGAATAGAGG CGAGGCAGCC AAATCTCAAA CGCGTGGCCC GCCCAGCTGC TACATCATGG 
AAGAAGCTGG AATTGTCTCG GGAGTATCAG AATGGCAACC AGTTGAGAGA ATACCAGCTG 
GAAGGTGTTA ATTGGCTTCT CTTTAACTGG TACAATAGGC AAAACTGCAT TTTGGCAGAT 
GAAATGGGTT TGGGGAAGAC CATCCAATCT ATCACATTCT TACAAGAAGT ATATAATGTG 
GGAATACGGG GACCTTTTTT AGTAATTGCT CCACTTTCCA CCATCACGAA CTGGGAGAGA 
GAGTTTGGCA GTTGGACTCA AATGAATACT ATTGTGTATC ATGGCAGCCT TGCTAGTCGC 
CAAATGATCC AGCAATATGA GATGTACTGT AAGGACTCCA AGGGTCGTCT TATTCCTGGT 
GCGTACAAGT TTGATGCTCT TATTACAACT TTTGAAATGG TGCTGTCTGA TTGCCCAGAG 
CTGCGTGAAA TTGAGTGGCG CTGTGTCATA ATTGATGAGG CTCATCGGCT CAAAAACAGA 
AACTGCAAAC TACTGGACAG CCTAAAGCAC ATGGACTTGG AGCACAAAGT CCTTTTAACA 
GGCACTCCAT TACAGAACAC CGTGGAAGAG TTGTTCAGTC TTTTGCATTT CCTGGAGCCA 
ACACAATTTT CCTCTGAAGC AGAGTTTCTT AAGGACTTTG GAGACCTGAA GACAGAGGAA 
CAGGTGCAGA AATTACAGGC CATCTTAAAA CCTATGATGT TGAGGCGATT AAAGGAAGAT 
GTTGAGAAAA ACCTGGCCCC GAAACAAGAA ACTATAATTG AGGTTGAGCT TACTAACATC 
CAGAAGAAGT ATTACCGAGC AATACTGGAG AAGAATTTTT CTTTTCTGAC CAAAGGAGCT 
AGTCAATCCA ACACACCCAA CTTGCTCAAT ACCATGATGG AACTACGCAA ATGCTGCAAC 
CACCCATACC TCATCACTGG AGCAGAAGAA AAGATCATAT CTGAGTTCCG AGAAGCAACT 
CCAGTAGTTC CCCCTGACTT TCACGTCCAA GCAATGGTAC GCTCCTCTGG CAAACTGGTG 
CTAATAGATA AGCTGCTCCC TAAACTAAGG GCTGGAGGCC ACAAAGTTCT CATCTTTTCT 
CAGATGGTAC GGTGCCTGGA CATCCTGGAA GACTATCTCA TTCAGAGAAG GTACCTTTAT 
GAGCGTATTG ATGGACGTGT CCGTGGCAAC ATGCGTCAGG CAGCCATTGA TAGATTCAGC 
CGCCCAGATT CTGACCGATT TGTGTTTCTA CTCTGTACAC GAGCTGGTGG GCTTGGAATT 
AACTTGACAG CTGCTGATAC TTGTATTATA TTTGACTCGG ACTGGAACCC TCAAAATGAC 
CTTCAGGCTC AAGCTCGATG TCATCGTATT GGGCAAAGCA AAGCCGTTAA AATTTACAGA 
CTCATCACTC GCAACTCCTA TGAGAGAGAG ATGTTTGATA AGGCCAGTCT CAAACTAGGA 
CTAGACAAAG CTGTATTGCA GTCTATGAGT GGTCGGGATA ATCACCTTAG TGGGCCTATT 
CAGCAATTCA CAAAGAAAGA AATTGAAGAC TTGCTAAGGA AAGGAGCTTA TGCTGCAATC 
ATGGATGAAG ATGACGAAGG CTCTAAATTC TGTGAAGAAG ATATTGACCA AATTTTACTG 
AGGCGTACCA CGACTATTAC CATAGAGTCA GAGGGAAAGG GCTCAACCTT CTCCAAGGCA 
AGTTTTGTTG CTTCTGAGAA CAGGACTGAT ATATCCCTAG ATGATCCTAA TTTCTGGCAA 
AAGTGGGCCA AGAAAGCAGA CTTAGACTTG GATCTTCTTA GCAGCAAGAA TACACTGGTT 
ATTGATACCC CACGTATCAG GAAGCAAACA CGGCACTTTA CTAACAAAGA TGATGACATG 
GTTGAGTTCT CAGATTTGGA GAGTGATGAT GATGATCGCC CCAAAGCTCG GCGTCAAGAC 
CGCAAGCATG GATATGGACG TACAGATTGC TTCCGTGTGG AGAAGCATTT GCTTGTTTAT 
GGATGGGGTC GCTGGCGAGA TATCCTTACT CATGGTCGTT TCAAGCGTGG TATGAATGAG 
CGTGACGTGG AGAAAATATG TAGAGCCATA CTGGTTTATT GTCTTTTGCA CTACCGGGGA 
GATGAAAACA TAAAGAGCTT TATCTGGGAT CTAATTACAC CAGCTGAGAA TGGCAAAACT 
AAGCAGCTAC AGAACCACTC TGGATTGTCT ATTCCTGTCC CCCGTGGTCG TAAGGGGAAA 
AAAGTGAAAT CCCAAAGCAG CTTTGACATA CATAAAGCCG ATTGGATACA TAAGTACAAC 
CCAGATACCT TGTTCCAAGA TGAAGGGTAC AAGAAACATC TCAAGCATCA ATGTAACAAG 
GTGCTGCTTC GCGTTCGTAT GTTATATTTT CTTCGTCAGG AAGTTATTGG AAACCAGGCA 
ATGAAAGTTT TAAGTGGTGT AGAGGCCAGG CAAATTGACA TCTGGTTCCC ACAGGTGGAC 
CAGGTTGAAG TTCCCTCACG CTGGTGGGAC AGAGAAGCTG ATAAATCTCT TCTTATCGGG 
GTCTTTAAAC ATGGTTATGA GAAGTATAAC ACCATGCGAG CTGACCCTGC ACTCTGTTTT 
TTGGAGAAGG TGGGGGGACC AGATGAACAG GCTATTGCAG CTGAGCACCA TGCTCAAGAC 
TTTTCTGAAC TCCCTGATGG TGGGGACTTT GATCGAGACA TAGAAGATCC AGAATACAAG 
CCACTTCATG CACAGAAAGA TCCTGAGGAT GAGATCGACT CTTTAATGAT GGATGAAGAA 
ATCTCTGTTG TTGACGGTGA AGAAGCAACA GCAATGCCCA GTGGTATGTT ATGGCCACCT 
GGCTCTGCTC TCACAGCTCG TCTACGGCGT CTAATTACTG CCTACCAACG CAGCTACAAA 
AGAGAACAAC TAAAATTGGA GGCTGAAGAG CGTGGGGACA AACGTAGAAA ACGATGTGAG 
GCAGCCTTTA AACTGAAAGA AATTGCCCGT AGGGAGAAAC AGCAAAGGTG GACCCGAAGG 
GAGGCATGTG ACTTTTTTCG TGTGCTGTCC AGCTTTGGTG TCGAGTATGA CCCCGATACT 
CAACTGTATG ACTGGCAGAG GTTCCGTGGT CTGGCAAGAC TGGACAAGAA GACAGATGAG 
TCACTGCTCA AATACTTCCA TGGATTTGTG GCAATGTGCC GCCAGGTTTG CCGCTTACCC 
CCTGCTGCCG GAGACGAGCC ACCAGATCCA TCTCTCTTCA TTGAGCCAAT TTCAGAAGAG 
CGAGCATCTC GCGCCCTGTT TCGCTTGGAT CTTCTTCGTA GGGTGCGTGA GCAAGTACTT 
TGTCATCCTT TGCTTATTCC TCGTTTGTCA CTGTGCCGGC CAGACCCAGA ACTCCCAGAA 
TGGTGGGAGA GTGGACGTCA TGACAATGAG CTTCTTCAGG GAGCAGCACG TTATGGTCTG 
AGCCGTACAG ACCTCACTAT ACTGCAGGAC CATGCATTTT CTTTTTTGCG GTGTCAGATC 
AGTTATTTCC AGTCAAGGGG AATATCTGGC CATTCTCGCC CTTCTACTCC AACTACTGCC 
CCTTCAGTGC CTACTGAACG TCAGCCTTCT CATCTAGCCT CCCTTTCCTC ATCTGTCAGC 
TGTTCTCCTG CTCCACGCAG ATCCTCTTCC TGCTCATCAT CATCTCACTC ATCAAACTCT 
GAGGATTCTT CAGATGAAAG CAATGGTGTA AAAGTAAAAC CTAATGGTCT TTCGCACGCT 
CGACTGTATG ATGAGGAGAG CCGGCTTTCT CTTACTGCCT CCCCTGCAGA TCTCACTACA 
GAGGACAGTA TTCAGACAGC GCTGGAGACC CCGCACAGTA CAGACTGGCC AAAGGACCGT 
GTCCTTATCT GCAGAATTGA GCAAGTATGC AGTGCCGTTC TTACTGGCAA ATGGTCCTCA 
CCACGACGAC TGTCAGATCC ACCAAGTGAC AGCCCTGATA GCTTGCCTCC AACCCCAGAG 
CAGCAGAGTC CAGCCCACTT CACACAGATA AGACCTGCAC CGGACCCCAA AGAGTTTACC 
ATTCAAATAA AAAGTGAAGA AGGCCTGAAG ATTAAATTTC AGAAGCACAA ATTTATGGGT 
AATGGAGCTC TTGATTCTGC ACCTCATACC CAGAAGAAAA AGAGCAAGAA AAAGATGGAT 
CTTGACATGG ACACACGAAT TCCCGTTGTA AACCAGAAGG ATGGGACCTT GTTGTTAGGG 
GAAGAAGCCC CTTTACGCTG CCAGCTGCCC GAGTGGTTAC ACAGAAATCC TGACTTCATG 
GTTGACCCAC GCTTTATTGC GTTTATGGAA GACCGCAGGA AAAGAAAAAA ACATCGAAGC 
AAAAAGTCCA AATCCTCCTC CCAGATTGGA AACGTACAGA ATCCCCACTC CCACAATGGA 
AAGAAGTCTT CCCACACAGA AAACTCTTTC GAGACGCAAC ACCTTCCTAC TGCAGCCAAA 
AAAAGGAAGA AACGCCCTCC TTCAAAAATA ATAATGCCAT CTGCACTACA TGGAGCTGGT 
GATGTTACAC ATTCCTTCTC AGACCTTCAA CCTGACCATT CTGGTCACCC CATGCGGTCA 
GTGTATGGAC CATCAGATGG AGGGCTGTCC CATCAAGCAG GACTCAGCGG GTTGGGATTT 
ACCCGCTTTC ATTCATCGCA GACATCTTCA GTGGGCAGAA TCCATGGCAC TACCTTTGAT 
GAGTTACAAA GTTTTGAGCC CCCACAACAA CATTTTTCTC TGCTTGATGA TCCTATGATG 
CCAGCAAATT CCTCCTCCAG CGGTGATGAA CTGGACAGAC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012812652.1
CDS	289..7293
Translation	MADPIMDLFDDPNLFGLDSLTGDSFGRDGPDTIDDALGLGNVLGPLEPITDRVGLPGADVGNNEVKQQTESQVLPDNPALMSATEIMQPIQLPTNQETLNQGNPFMGASNAGAPKIMILKAPAGMTVGACPVTQIQTLTPHQAANGGKVAFAKVLTGTPLRPGVSIVSGNTVLAKMPSTGVAGQVGAVRPVRQLLLQPVRASAAPGSSESNTGIKPAITLTSAPQQGDPKRITLVLQQPSQVGATAQQGQRHVVLGGLPGKIVLQGNQLAALTQAKTPQGQPAKVVTIQLQVQQQPGAAGQTPQKFQIVQQAPGGVAIAPSGQHTHMLNQQGVQRLSVPLKVVLQPQAGSSQGTAQGLSVVKVLNASEVANLTASQTVVKTSTGGGESRKLDSQKKQEKANRIVAEAIARARARGEQNIPRVLNEDELPSVNPEDDDGSRRKRKKKKGETSDRSKDEKPKKVKGSGSLRSRSKGKPSTITPIVGKKRKRNPSSDNSDAEIMASQASPAEDEDSVQKRRSNRQVKRKKYTEDLDIKITDDEDDEEVDVTGPVKTEPVLLPEPMPLPDSEAVPSMQFFVENPSEEDAAIVDKILSMRLAKKELPTGEYVEVEEYFVKYKNYSYLHCEWATIEQLERDKRIHQKLKRFKTKMTQMQHFLQEDEESFNPDYVEVDRILDESHSTDKDNGEPVVYYLVKWCSLPYEDSTWELKEDVDDGKIEEFKRIEARQPNLKRVARPAATSWKKLELSREYQNGNQLREYQLEGVNWLLFNWYNRQNCILADEMGLGKTIQSITFLQEVYNVGIRGPFLVIAPLSTITNWEREFGSWTQMNTIVYHGSLASRQMIQQYEMYCKDSKGRLIPGAYKFDALITTFEMVLSDCPELREIEWRCVIIDEAHRLKNRNCKLLDSLKHMDLEHKVLLTGTPLQNTVEELFSLLHFLEPTQFSSEAEFLKDFGDLKTEEQVQKLQAILKPMMLRRLKEDVEKNLAPKQETIIEVELTNIQKKYYRAILEKNFSFLTKGASQSNTPNLLNTMMELRKCCNHPYLITGAEEKIISEFREATPVVPPDFHVQAMVRSSGKLVLIDKLLPKLRAGGHKVLIFSQMVRCLDILEDYLIQRRYLYERIDGRVRGNMRQAAIDRFSRPDSDRFVFLLCTRAGGLGINLTAADTCIIFDSDWNPQNDLQAQARCHRIGQSKAVKIYRLITRNSYEREMFDKASLKLGLDKAVLQSMSGRDNHLSGPIQQFTKKEIEDLLRKGAYAAIMDEDDEGSKFCEEDIDQILLRRTTTITIESEGKGSTFSKASFVASENRTDISLDDPNFWQKWAKKADLDLDLLSSKNTLVIDTPRIRKQTRHFTNKDDDMVEFSDLESDDDDRPKARRQDRKHGYGRTDCFRVEKHLLVYGWGRWRDILTHGRFKRGMNERDVEKICRAILVYCLLHYRGDENIKSFIWDLITPAENGKTKQLQNHSGLSIPVPRGRKGKKVKSQSSFDIHKADWIHKYNPDTLFQDEGYKKHLKHQCNKVLLRVRMLYFLRQEVIGNQAMKVLSGVEARQIDIWFPQVDQVEVPSRWWDREADKSLLIGVFKHGYEKYNTMRADPALCFLEKVGGPDEQAIAAEHHAQDFSELPDGGDFDRDIEDPEYKPLHAQKDPEDEIDSLMMDEEISVVDGEEATAMPSGMLWPPGSALTARLRRLITAYQRSYKREQLKLEAEERGDKRRKRCEAAFKLKEIARREKQQRWTRREACDFFRVLSSFGVEYDPDTQLYDWQRFRGLARLDKKTDESLLKYFHGFVAMCRQVCRLPPAAGDEPPDPSLFIEPISEERASRALFRLDLLRRVREQVLCHPLLIPRLSLCRPDPELPEWWESGRHDNELLQGAARYGLSRTDLTILQDHAFSFLRCQISYFQSRGISGHSRPSTPTTAPSVPTERQPSHLASLSSSVSCSPAPRRSSSCSSSSHSSNSEDSSDESNGVKVKPNGLSHARLYDEESRLSLTASPADLTTEDSIQTALETPHSTDWPKDRVLICRIEQVCSAVLTGKWSSPRRLSDPPSDSPDSLPPTPEQQSPAHFTQIRPAPDPKEFTIQIKSEEGLKIKFQKHKFMGNGALDSAPHTQKKKSKKKMDLDMDTRIPVVNQKDGTLLLGEEAPLRCQLPEWLHRNPDFMVDPRFIAFMEDRRKRKKHRSKKSKSSSQIGNVQNPHSHNGKKSSHTENSFETQHLPTAAKKRKKRPPSKIIMPSALHGAGDVTHSFSDLQPDHSGHPMRSVYGPSDGGLSHQAGLSGLGFTRFHSSQTSSVGRIHGTTFDELQSFEPPQQHFSLLDDPMMPANSSSSGDELDRP

Target ORF information:

RefSeq Version	XM_012957198.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis chromodomain helicase DNA binding protein 8 (chd8), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957198.3

ORF Insert Sequence:

ATGGCTGACC CTATCATGGA CCTCTTTGAT GATCCAAACC TATTTGGCCT TGACTCTCTC 
ACTGGAGATT CCTTTGGACG GGATGGGCCA GACACTATAG ATGATGCTCT AGGCCTGGGT 
AATGTCTTGG GACCTTTGGA GCCGATAACA GACAGAGTTG GCCTGCCTGG TGCTGATGTT 
GGCAATAATG AAGTTAAACA ACAAACAGAG TCACAGGTTC TCCCGGACAA TCCAGCTCTT 
ATGTCAGCTA CTGAGATCAT GCAGCCCATT CAACTTCCCA CAAACCAGGA AACACTGAAC 
CAAGGAAATC CCTTTATGGG TGCCTCAAAT GCTGGAGCCC CAAAAATTAT GATTCTGAAA 
GCTCCTGCTG GAATGACAGT TGGTGCATGT CCTGTAACTC AGATACAAAC TCTGACACCT 
CACCAAGCTG CTAATGGTGG CAAGGTGGCA TTTGCTAAGG TGCTTACAGG AACCCCATTG 
CGTCCTGGGG TCTCAATTGT ATCTGGAAAC ACTGTCCTTG CCAAAATGCC AAGCACTGGA 
GTTGCAGGGC AGGTAGGAGC AGTGAGGCCT GTCCGTCAGC TACTTCTTCA ACCAGTGCGT 
GCCTCCGCAG CCCCAGGAAG CTCCGAGAGT AATACAGGGA TCAAGCCTGC AATCACACTA 
ACGTCAGCAC CTCAACAGGG GGACCCTAAA AGAATTACTC TCGTCCTACA GCAGCCTTCC 
CAAGTTGGTG CAACTGCACA ACAAGGGCAG CGTCATGTTG TGCTGGGTGG GCTTCCAGGA 
AAGATTGTCC TTCAGGGAAA CCAACTGGCA GCTCTCACAC AAGCTAAAAC CCCTCAAGGT 
CAGCCAGCTA AAGTAGTGAC TATCCAGCTG CAAGTCCAGC AGCAGCCTGG GGCTGCAGGT 
CAGACTCCTC AAAAGTTTCA GATTGTACAA CAAGCCCCAG GAGGTGTGGC AATTGCTCCC 
AGTGGGCAAC ATACACACAT GCTTAATCAA CAAGGAGTTC AGAGGCTTTC TGTTCCACTC 
AAAGTTGTTC TGCAACCACA GGCTGGTTCC TCTCAGGGTA CTGCGCAGGG CCTGTCAGTT 
GTGAAAGTTC TAAATGCAAG TGAAGTTGCC AATTTGACTG CAAGTCAGAC TGTGGTTAAA 
ACATCCACTG GAGGTGGTGA ATCTCGTAAG CTGGACAGCC AGAAGAAGCA AGAGAAAGCT 
AACCGTATTG TAGCTGAAGC TATTGCCAGG GCCCGAGCCA GAGGTGAACA GAATATTCCT 
CGGGTCCTGA ATGAGGATGA GCTTCCCAGT GTGAATCCTG AAGATGATGA TGGAAGCAGG 
CGGAAAAGGA AGAAGAAGAA AGGAGAGACT AGTGATAGGT CCAAGGATGA GAAACCTAAG 
AAGGTCAAAG GTAGTGGAAG CTTAAGGTCT AGGAGCAAAG GCAAACCAAG TACTATCACT 
CCTATTGTGG GTAAGAAAAG GAAGAGAAAC CCATCTTCCG ATAATTCTGA TGCTGAGATC 
ATGGCATCAC AGGCTTCTCC TGCAGAGGAT GAAGACAGTG TCCAGAAGAG ACGTTCAAAT 
CGCCAGGTGA AGCGCAAGAA GTATACAGAG GATTTGGATA TTAAAATAAC AGATGATGAA 
GATGATGAGG AGGTAGATGT GACGGGACCT GTGAAAACAG AACCTGTGCT GCTTCCTGAG 
CCCATGCCTT TACCTGACAG TGAGGCAGTC CCTTCAATGC AGTTCTTCGT GGAGAACCCA 
AGTGAAGAAG ATGCTGCTAT TGTTGACAAA ATACTCTCTA TGCGCCTAGC TAAAAAAGAG 
CTGCCCACTG GAGAGTATGT TGAAGTTGAA GAGTACTTTG TCAAATATAA AAATTATTCC 
TACCTACACT GTGAATGGGC CACAATTGAG CAACTGGAAA GGGACAAGCG TATTCATCAG 
AAACTGAAAA GATTTAAGAC AAAGATGACC CAGATGCAGC ATTTCTTGCA AGAAGATGAA 
GAATCTTTCA ATCCAGACTA TGTAGAGGTA GATCGAATCC TAGACGAATC TCACAGCACT 
GACAAGGACA ATGGTGAACC GGTGGTGTAT TACCTAGTAA AATGGTGCTC TTTACCTTAT 
GAAGATAGCA CATGGGAGCT GAAAGAAGAT GTTGATGATG GAAAGATAGA GGAATTCAAA 
CGAATAGAGG CGAGGCAGCC AAATCTCAAA CGCGTGGCCC GCCCAGCTGC TACATCATGG 
AAGAAGCTGG AATTGTCTCG GGAGTATCAG AATGGCAACC AGTTGAGAGA ATACCAGCTG 
GAAGGTGTTA ATTGGCTTCT CTTTAACTGG TACAATAGGC AAAACTGCAT TTTGGCAGAT 
GAAATGGGTT TGGGGAAGAC CATCCAATCT ATCACATTCT TACAAGAAGT ATATAATGTG 
GGAATACGGG GACCTTTTTT AGTAATTGCT CCACTTTCCA CCATCACGAA CTGGGAGAGA 
GAGTTTGGCA GTTGGACTCA AATGAATACT ATTGTGTATC ATGGCAGCCT TGCTAGTCGC 
CAAATGATCC AGCAATATGA GATGTACTGT AAGGACTCCA AGGGTCGTCT TATTCCTGGT 
GCGTACAAGT TTGATGCTCT TATTACAACT TTTGAAATGG TGCTGTCTGA TTGCCCAGAG 
CTGCGTGAAA TTGAGTGGCG CTGTGTCATA ATTGATGAGG CTCATCGGCT CAAAAACAGA 
AACTGCAAAC TACTGGACAG CCTAAAGCAC ATGGACTTGG AGCACAAAGT CCTTTTAACA 
GGCACTCCAT TACAGAACAC CGTGGAAGAG TTGTTCAGTC TTTTGCATTT CCTGGAGCCA 
ACACAATTTT CCTCTGAAGC AGAGTTTCTT AAGGACTTTG GAGACCTGAA GACAGAGGAA 
CAGGTGCAGA AATTACAGGC CATCTTAAAA CCTATGATGT TGAGGCGATT AAAGGAAGAT 
GTTGAGAAAA ACCTGGCCCC GAAACAAGAA ACTATAATTG AGGTTGAGCT TACTAACATC 
CAGAAGAAGT ATTACCGAGC AATACTGGAG AAGAATTTTT CTTTTCTGAC CAAAGGAGCT 
AGTCAATCCA ACACACCCAA CTTGCTCAAT ACCATGATGG AACTACGCAA ATGCTGCAAC 
CACCCATACC TCATCACTGG AGCAGAAGAA AAGATCATAT CTGAGTTCCG AGAAGCAACT 
CCAGTAGTTC CCCCTGACTT TCACGTCCAA GCAATGGTAC GCTCCTCTGG CAAACTGGTG 
CTAATAGATA AGCTGCTCCC TAAACTAAGG GCTGGAGGCC ACAAAGTTCT CATCTTTTCT 
CAGATGGTAC GGTGCCTGGA CATCCTGGAA GACTATCTCA TTCAGAGAAG GTACCTTTAT 
GAGCGTATTG ATGGACGTGT CCGTGGCAAC ATGCGTCAGG CAGCCATTGA TAGATTCAGC 
CGCCCAGATT CTGACCGATT TGTGTTTCTA CTCTGTACAC GAGCTGGTGG GCTTGGAATT 
AACTTGACAG CTGCTGATAC TTGTATTATA TTTGACTCGG ACTGGAACCC TCAAAATGAC 
CTTCAGGCTC AAGCTCGATG TCATCGTATT GGGCAAAGCA AAGCCGTTAA AATTTACAGA 
CTCATCACTC GCAACTCCTA TGAGAGAGAG ATGTTTGATA AGGCCAGTCT CAAACTAGGA 
CTAGACAAAG CTGTATTGCA GTCTATGAGT GGTCGGGATA ATCACCTTAG TGGGCCTATT 
CAGCAATTCA CAAAGAAAGA AATTGAAGAC TTGCTAAGGA AAGGAGCTTA TGCTGCAATC 
ATGGATGAAG ATGACGAAGG CTCTAAATTC TGTGAAGAAG ATATTGACCA AATTTTACTG 
AGGCGTACCA CGACTATTAC CATAGAGTCA GAGGGAAAGG GCTCAACCTT CTCCAAGGCA 
AGTTTTGTTG CTTCTGAGAA CAGGACTGAT ATATCCCTAG ATGATCCTAA TTTCTGGCAA 
AAGTGGGCCA AGAAAGCAGA CTTAGACTTG GATCTTCTTA GCAGCAAGAA TACACTGGTT 
ATTGATACCC CACGTATCAG GAAGCAAACA CGGCACTTTA CTAACAAAGA TGATGACATG 
GTTGAGTTCT CAGATTTGGA GAGTGATGAT GATGATCGCC CCAAAGCTCG GCGTCAAGAC 
CGCAAGCATG GATATGGACG TACAGATTGC TTCCGTGTGG AGAAGCATTT GCTTGTTTAT 
GGATGGGGTC GCTGGCGAGA TATCCTTACT CATGGTCGTT TCAAGCGTGG TATGAATGAG 
CGTGACGTGG AGAAAATATG TAGAGCCATA CTGGTTTATT GTCTTTTGCA CTACCGGGGA 
GATGAAAACA TAAAGAGCTT TATCTGGGAT CTAATTACAC CAGCTGAGAA TGGCAAAACT 
AAGCAGCTAC AGAACCACTC TGGATTGTCT ATTCCTGTCC CCCGTGGTCG TAAGGGGAAA 
AAAGTGAAAT CCCAAAGCAG CTTTGACATA CATAAAGCCG ATTGGATACA TAAGTACAAC 
CCAGATACCT TGTTCCAAGA TGAAGGGTAC AAGAAACATC TCAAGCATCA ATGTAACAAG 
GTGCTGCTTC GCGTTCGTAT GTTATATTTT CTTCGTCAGG AAGTTATTGG AAACCAGGCA 
ATGAAAGTTT TAAGTGGTGT AGAGGCCAGG CAAATTGACA TCTGGTTCCC ACAGGTGGAC 
CAGGTTGAAG TTCCCTCACG CTGGTGGGAC AGAGAAGCTG ATAAATCTCT TCTTATCGGG 
GTCTTTAAAC ATGGTTATGA GAAGTATAAC ACCATGCGAG CTGACCCTGC ACTCTGTTTT 
TTGGAGAAGG TGGGGGGACC AGATGAACAG GCTATTGCAG CTGAGCACCA TGCTCAAGAC 
TTTTCTGAAC TCCCTGATGG TGGGGACTTT GATCGAGACA TAGAAGATCC AGAATACAAG 
CCACTTCATG CACAGAAAGA TCCTGAGGAT GAGATCGACT CTTTAATGAT GGATGAAGAA 
ATCTCTGTTG TTGACGGTGA AGAAGCAACA GCAATGCCCA GTGGTATGTT ATGGCCACCT 
GGCTCTGCTC TCACAGCTCG TCTACGGCGT CTAATTACTG CCTACCAACG CAGCTACAAA 
AGAGAACAAC TAAAATTGGA GGCTGAAGAG CGTGGGGACA AACGTAGAAA ACGATGTGAG 
GCAGCCTTTA AACTGAAAGA AATTGCCCGT AGGGAGAAAC AGCAAAGGTG GACCCGAAGG 
GAGGCATGTG ACTTTTTTCG TGTGCTGTCC AGCTTTGGTG TCGAGTATGA CCCCGATACT 
CAACTGTATG ACTGGCAGAG GTTCCGTGGT CTGGCAAGAC TGGACAAGAA GACAGATGAG 
TCACTGCTCA AATACTTCCA TGGATTTGTG GCAATGTGCC GCCAGGTTTG CCGCTTACCC 
CCTGCTGCCG GAGACGAGCC ACCAGATCCA TCTCTCTTCA TTGAGCCAAT TTCAGAAGAG 
CGAGCATCTC GCGCCCTGTT TCGCTTGGAT CTTCTTCGTA GGGTGCGTGA GCAAGTACTT 
TGTCATCCTT TGCTTATTCC TCGTTTGTCA CTGTGCCGGC CAGACCCAGA ACTCCCAGAA 
TGGTGGGAGA GTGGACGTCA TGACAATGAG CTTCTTCAGG GAGCAGCACG TTATGGTCTG 
AGCCGTACAG ACCTCACTAT ACTGCAGGAC CATGCATTTT CTTTTTTGCG GTGTCAGATC 
AGTTATTTCC AGTCAAGGGG AATATCTGGC CATTCTCGCC CTTCTACTCC AACTACTGCC 
CCTTCAGTGC CTACTGAACG TCAGCCTTCT CATCTAGCCT CCCTTTCCTC ATCTGTCAGC 
TGTTCTCCTG CTCCACGCAG ATCCTCTTCC TGCTCATCAT CATCTCACTC ATCAAACTCT 
GAGGATTCTT CAGATGAAAG CAATGGTGTA AAAGTAAAAC CTAATGGTCT TTCGCACGCT 
CGACTGTATG ATGAGGAGAG CCGGCTTTCT CTTACTGCCT CCCCTGCAGA TCTCACTACA 
GAGGACAGTA TTCAGACAGC GCTGGAGACC CCGCACAGTA CAGACTGGCC AAAGGACCGT 
GTCCTTATCT GCAGAATTGA GCAAGTATGC AGTGCCGTTC TTACTGGCAA ATGGTCCTCA 
CCACGACGAC TGTCAGATCC ACCAAGTGAC AGCCCTGATA GCTTGCCTCC AACCCCAGAG 
CAGCAGAGTC CAGCCCACTT CACACAGATA AGACCTGCAC CGGACCCCAA AGAGTTTACC 
ATTCAAATAA AAAGTGAAGA AGGCCTGAAG ATTAAATTTC AGAAGCACAA ATTTATGGGT 
AATGGAGCTC TTGATTCTGC ACCTCATACC CAGAAGAAAA AGAGCAAGAA AAAGATGGAT 
CTTGACATGG ACACACGAAT TCCCGTTGTA AACCAGAAGG ATGGGACCTT GTTGTTAGGG 
GAAGAAGCCC CTTTACGCTG CCAGCTGCCC GAGTGGTTAC ACAGAAATCC TGACTTCATG 
GTTGACCCAC GCTTTATTGC GTTTATGGAA GACCGCAGGA AAAGAAAAAA ACATCGAAGC 
AAAAAGTCCA AATCCTCCTC CCAGATTGGA AACGTACAGA ATCCCCACTC CCACAATGGA 
AAGAAGTCTT CCCACACAGA AAACTCTTTC GAGACGCAAC ACCTTCCTAC TGCAGCCAAA 
AAAAGGAAGA AACGCCCTCC TTCAAAAATA ATAATGCCAT CTGCACTACA TGGAGCTGGT 
GATGTTACAC ATTCCTTCTC AGACCTTCAA CCTGACCATT CTGGTCACCC CATGCGGTCA 
GTGTATGGAC CATCAGATGG AGGGCTGTCC CATCAAGCAG GACTCAGCGG GTTGGGATTT 
ACCCGCTTTC ATTCATCGCA GACATCTTCA GTGGGCAGAA TCCATGGCAC TACCTTTGAT 
GAGTTACAAA GTTTTGAGCC CCCACAACAA CATTTTTCTC TGCTTGATGA TCCTATGATG 
CCAGCAAATT CCTCCTCCAG CGGTGATGAA CTGGACAGAC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chd8 ( NM_001137617.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001131089.2 Xenopus tropicalis chromodomain helicase DNA binding protein 8 (chd8), mRNA.

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CloneID	OXa01041
Clone ID Related Accession (Same CDS sequence)	NM_001137617.2
Accession Version	NM_001137617.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6555bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Xenopus tropicalis(tropical clawed frog)
Product	chromodomain-helicase-DNA-binding protein 8
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC168549.1, EL843535.1 and DN017167.1. On Oct 8, 2011 this sequence version replaced NM_001137617.1. ##Evidence-Data-START## Transcript exon combination :: BC168549.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN05930350, SAMN06679842 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGACC CTATCATGGA CCTCTTTGAT GATCCAAACC TATTTGGCCT TGACTCTCTC 
ACTGGAGATT CCTTTGGACG GGATGGGCCA GACACTATAG ATGATGCTCT AGGCCTGGGT 
AATGTCTTGG GACCTTTGGA GCCGATAACA GACAGAGTTG GCCTGCCTGG TGCTGATGTT 
GGCAATAATG AAGTTAAACA ACAAACAGAG TCACAGGTTC TCCCGGACAA TCCAGCTCTT 
ATGTCAGCTA CTGAGATCAT GCAGCCCATT CAACTTCCCA CAAACCAGGA AACACTGAAC 
CAAGGAAATC CCTTTATGGG TGCCTCAAAT GCTGGAGCCC CAAAAATTAT GATTCTGAAA 
GCTCCTGCTG GAATGACAGT TGGTGCATGT CCTGTAACTC AGATACAAAC TCTGACACCT 
CACCAAGCTG CTAATGGTGG CAAGGTGGCA TTTGCTAAGG TGCTTACAGG AACCCCATTG 
CGTCCTGGGG TCTCAATTGT ATCTGGAAAC ACTGTCCTTG CCAAAATGCC AAGCACTGGA 
GTTGCAGGGC AGGTAGGAGC AGTGAGGCCT GTCCGTCAGC TACTTCTTCA ACCAGTGCGT 
GCCTCCGCAG CCCCAGGAAG CTCCGAGAGT AATACAGGGA TCAAGCCTGC AATCACACTA 
ACGTCAGCAC CTCAACAGGG GGACCCTAAA AGAATTACTC TCGTCCTACA GCAGCCTTCC 
CAAGTTGGTG CAACTGCACA ACAAGGGCAG CGTCATGTTG TGCTGGGTGG GCTTCCAGGA 
AAGATTGTCC TTCAGGGAAA CCAACTGGCA GCTCTCACAC AAGCTAAAAC CCCTCAAGGT 
CAGCCAGCTA AAGTAGTGAC TATCCAGCTG CAAGTCCAGC AGCAGCCTGG GGCTGCAGGT 
CAGACTCCTC AAAAGTTTCA GATTGTACAA CAAGCCCCAG GAGGTGTGGC AATTGCTCCC 
AGTGGGCAAC ATACACACAT GCTTAATCAA CAAGGAGTTC AGAGGCTTTC TGTTCCACTC 
AAAGTTGTTC TGCAACCACA GGCTGGTTCC TCTCAGGGTA CTGCGCAGGG CCTGTCAGTT 
GTGAAAGTTC TAAATGCAAG TGAAGTTGCC AATTTGACTG CAAGTCAGAC TGTGGTTAAA 
ACATCCACTG GAGGTGGTGA ATCTCGTAAG CTGGACAGCC AGAAGAAGCA AGAGAAAGCT 
AACCGTATTG TAGCTGAAGC TATTGCCAGG GCCCGAGCCA GAGGTGAACA GAATATTCCT 
CGGGTCCTGA ATGAGGATGA GCTTCCCAGT GTGAATCCTG AAGATGATGA TGGAAGCAGG 
CGGAAAAGGA AGAAGAAGAA AGGAGAGACT AGTGATAGGT CCAAGGATGA GAAACCTAAG 
AAGGTCAAAG GTAGTGGAAG CTTAAGGTCT AGGAGCAAAG GCAAACCAAG TACTATCACT 
CCTATTGTGG GTAAGAAAAG GAAGAGAAAC CCATCTTCCG ATAATTCTGA TGCTGAGATC 
ATGGCATCAC AGGCTTCTCC TGCAGAGGAT GAAGACAGTG TCCAGAAGAG ACGTTCAAAT 
CGCCAGGTGA AGCGCAAGAA GTATACAGAG GATTTGGATA TTAAAATAAC AGATGATGAA 
GATGATGAGG AGGTAGATGT GACGGGACCT GTGAAAACAG AACCTGTGCT GCTTCCTGAG 
CCCATGCCTT TACCTGACAG TGAGGCAGTC CCTTCAATGC AGTTCTTCGT GGAGAACCCA 
AGTGAAGAAG ATGCTGCTAT TGTTGACAAA ATACTCTCTA TGCGCCTAGC TAAAAAAGAG 
CTGCCCACTG GAGAGTATGT TGAAGTTGAA GAGTACTTTG TCAAATATAA AAATTATTCC 
TACCTACACT GTGAATGGGC CACAATTGAG CAACTGGAAA GGGACAAGCG TATTCATCAG 
AAACTGAAAA GATTTAAGAC AAAGATGACC CAGATGCAGC ATTTCTTGCA AGAAGATGAA 
GAATCTTTCA ATCCAGACTA TGTAGAGGTA GATCGAATCC TAGACGAATC TCACAGCACT 
GACAAGGACA ATGGTGAACC GGTGGTGTAT TACCTAGTAA AATGGTGCTC TTTACCTTAT 
GAAGATAGCA CATGGGAGCT GAAAGAAGAT GTTGATGATG GAAAGATAGA GGAATTCAAA 
CGAATAGAGG CGAGGCAGCC AAATCTCAAA CGCGTGGCCC GCCCAGCTGC TACATCATGG 
AAGAAGCTGG AATTGTCTCG GGAGTATCAG AATGGCAACC AGTTGAGAGA ATACCAGCTG 
GAAGGTGTTA ATTGGCTTCT CTTTAACTGG TACAATAGGC AAAACTGCAT TTTGGCAGAT 
GAAATGGGTT TGGGGAAGAC CATCCAATCT ATCACATTCT TACAAGAAGT ATATAATGTG 
GGAATACGGG GACCTTTTTT AGTAATTGCT CCACTTTCCA CCATCACGAA CTGGGAGAGA 
GAGTTTGGCA GTTGGACTCA AATGAATACT ATTGTGTATC ATGGCAGCCT TGCTAGTCGC 
CAAATGATCC AGCAATATGA GATGTACTGT AAGGACTCCA AGGGTCGTCT TATTCCTGGT 
GCGTACAAGT TTGATGCTCT TATTACAACT TTTGAAATGG TGCTGTCTGA TTGCCCAGAG 
CTGCGTGAAA TTGAGTGGCG CTGTGTCATA ATTGATGAGG CTCATCGGCT CAAAAACAGA 
AACTGCAAAC TACTGGACAG CCTAAAGCAC ATGGACTTGG AGCACAAAGT CCTTTTAACA 
GGCACTCCAT TACAGAACAC CGTGGAAGAG TTGTTCAGTC TTTTGCATTT CCTGGAGCCA 
ACACAATTTT CCTCTGAAGC AGAGTTTCTT AAGGACTTTG GAGACCTGAA GACAGAGGAA 
CAGGTGCAGA AATTACAGGC CATCTTAAAA CCTATGATGT TGAGGCGATT AAAGGAAGAT 
GTTGAGAAAA ACCTGGCCCC GAAACAAGAA ACTATAATTG AGGTTGAGCT TACTAACATC 
CAGAAGAAGT ATTACCGAGC AATACTGGAG AAGAATTTTT CTTTTCTGAC CAAAGGAGCT 
AGTCAATCCA ACACACCCAA CTTGCTCAAT ACCATGATGG AACTACGCAA ATGCTGCAAC 
CACCCATACC TCATCACTGG AGCAGAAGAA AAGATCATAT CTGAGTTCCG AGAAGCAACT 
CCAGTAGTTC CCCCTGACTT TCACGTCCAA GCAATGGTAC GCTCCTCTGG CAAACTGGTG 
CTAATAGATA AGCTGCTCCC TAAACTAAGG GCTGGAGGCC ACAAAGTTCT CATCTTTTCT 
CAGATGGTAC GGTGCCTGGA CATCCTGGAA GACTATCTCA TTCAGAGAAG GTACCTTTAT 
GAGCGTATTG ATGGACGTGT CCGTGGCAAC ATGCGTCAGG CAGCCATTGA TAGATTCAGC 
CGCCCAGATT CTGACCGATT TGTGTTTCTA CTCTGTACAC GAGCTGGTGG GCTTGGAATT 
AACTTGACAG CTGCTGATAC TTGTATTATA TTTGACTCGG ACTGGAACCC TCAAAATGAC 
CTTCAGGCTC AAGCTCGATG TCATCGTATT GGGCAAAGCA AAGCCGTTAA AATTTACAGA 
CTCATCACTC GCAACTCCTA TGAGAGAGAG ATGTTTGATA AGGCCAGTCT CAAACTAGGA 
CTAGACAAAG CTGTATTGCA GTCTATGAGT GGTCGGGATA ATCACCTTAG TGGGCCTATT 
CAGCAATTCA CAAAGAAAGA AATTGAAGAC TTGCTAAGGA AAGGAGCTTA TGCTGCAATC 
ATGGATGAAG ATGACGAAGG CTCTAAATTC TGTGAAGAAG ATATTGACCA AATTTTACTG 
AGGCGTACCA CGACTATTAC CATAGAGTCA GAGGGAAAGG GCTCAACCTT CTCCAAGGCA 
AGTTTTGTTG CTTCTGAGAA CAGGACTGAT ATATCCCTAG ATGATCCTAA TTTCTGGCAA 
AAGTGGGCCA AGAAAGCAGA CTTAGACTTG GATCTTCTTA GCAGCAAGAA TACACTGGTT 
ATTGATACCC CACGTATCAG GAAGCAAACA CGGCACTTTA CTAACAAAGA TGATGACATG 
GTTGAGTTCT CAGATTTGGA GAGTGATGAT GATGATCGCC CCAAAGCTCG GCGTCAAGAC 
CGCAAGCATG GATATGGACG TACAGATTGC TTCCGTGTGG AGAAGCATTT GCTTGTTTAT 
GGATGGGGTC GCTGGCGAGA TATCCTTACT CATGGTCGTT TCAAGCGTGG TATGAATGAG 
CGTGACGTGG AGAAAATATG TAGAGCCATA CTGGTTTATT GTCTTTTGCA CTACCGGGGA 
GATGAAAACA TAAAGAGCTT TATCTGGGAT CTAATTACAC CAGCTGAGAA TGGCAAAACT 
AAGCAGCTAC AGAACCACTC TGGATTGTCT ATTCCTGTCC CCCGTGGTCG TAAGGGGAAA 
AAAGTGAAAT CCCAAAGCAG CTTTGACATA CATAAAGCCG ATTGGATACA TAAGTACAAC 
CCAGATACCT TGTTCCAAGA TGAAGGGTAC AAGAAACATC TCAAGCATCA ATGTAACAAG 
GTGCTGCTTC GCGTTCGTAT GTTATATTTT CTTCGTCAGG AAGTTATTGG AAACCAGGCA 
ATGAAAGTTT TAAGTGGTGT AGAGGCCAGG CAAATTGACA TCTGGTTCCC ACAGGTGGAC 
CAGGTTGAAG TTCCCTCGCG CTGGTGGGAC AGAGAAGCTG ATAAATCTCT TCTTATCGGG 
GTCTTTAAAC ATGGTTATGA GAAGTATAAC ACCATGCGAG CTGACCCTGC ACTCTGTTTT 
TTGGAGAAGG TGGGGGGACC AGATGAACAG GCTATTGCAG CTGAGCACCA TGCTCAAGAC 
TTTTCTGAAC TCCCTGATGG TGGGGACTTT GATCGAGACA TAGAAGATCC AGAATACAAG 
CCACTTCATG CACAGAAAGA TCCTGAGGAT GAGATCGACT CTTTAATGAT GGATGAAGAA 
ATCTCTGTTG TTGACGGTGA AGAAGCAACA GCAATGCCCA GTGGTATGTT ATGGCCACCT 
GGCTCTGCTC TCACAGCTCG TCTACGGCGT CTAATTACTG CCTACCAACG CAGCTACAAA 
AGAGAACAAC TAAAATTGGA GGCTGAAGAG CGTGGGGACA AACGTAGAAA ACGATGTGAG 
GCAGCCTTTA AACTGAAAGA AATTGCCCGT AGGGAGAAAC AGCAAAGGTG GACCCGAAGG 
GAGGCATGTG ACTTTTTTCG TGTGCTGTCC AGCTTTGGTG TCGAGTATGA CCCCGATACT 
CAACTGTATG ACTGGCAGAG GTTCCGTGGT CTGGCAAGAC TGGACAAGAA GACAGATGAG 
TCACTGCTCA AATACTTCCA TGGATTTGTG GCAATGTGCC GCCAGGTTTG CCGCTTACCC 
CCTGCTGCCG GAGACGAGCC ACCAGATCCA TCTCTCTTCA TTGAGCCAAT TTCAGAAGAG 
CGAGCATCTC GCGCCCTGTT TCGCTTGGAT CTTCTTCGTA GGGTGCGTGA GCAAGTACTT 
TGTCATCCTT TGCTTATTCC TCGTTTGTCA CTGTGCCGGC CAGACCCAGA ACTCCCAGAA 
TGGTGGGAGA GTGGACGTCA TGACAATGAG CTTCTTCAGG GAGCAGCACG TTATGGTCTG 
AGCCGTACAG ACCTCACTAT ACTGCAGGAC CATGCATTTT CTTTTTTGCG GTGTCAGATC 
AGTTATTTCC AGTCAAGGGG AATATCTGGC CATTCTCGCC CTTCTACTCC AACTACTGCC 
CCTTCAGTGC CTACTGAACG TCAGCCTTCT CATCTAGCCT CCCTTTCCTC ATCTGTCAGC 
TGTTCTCCTG CTCCACGCAG ATCCTCTTCC TGCTCATCAT CATCTCACTC ATCAAACTCT 
GAGGATTCTT CAGATGAAAG CAATGGTGTA AAAGTAAAAC CTAATGCAGG TCTTTCGCAC 
GCTCGACTGT ATGATGAGGA GAGCCGGCTT TCTCTTACTG CCTCCCCTGC AGATCTCACT 
ACAGAGGACA GTATTCAGAC AGCGCTGGAG ACCCCGCACA GTACAGACTG GCCAAAGGAC 
CGTGTCCTTA TCTGCAGAAT TGAGCAAGTA TGCAGTGCCG TTCTTACTGG CAAATGGTCC 
TCACCACGAC GACTGTCAGA TCCACCAAGT GACAGCCCTG ATAGCTTGCC TCCAACCCCA 
GAGCAGCAGA GTCCAGCCCA CTTCACACAG ATAAGACCTG CACCGGACCC CAAAGAGTTT 
ACCATTCAAA TAAAAAGTGA AGAAGGCCTG AAGATTAAAT TTCAGAAGCA CAAATTTATG 
GGTAATGGAG CTCTTGATTC TGCACCTCAT ACCCAGAAGA AAAAGAGCAA GAAAAAGATG 
GATCTTGACA TGGACACACG AATTCCCGTT GTAAACCAGA AGGATGGGAC CTTGTTGTTA 
GGGGAAGAAG CCCCTTTACG CTGCCAGCTG CCCGAGTGGT TACACAGAAA TCCTGACTTC 
ATGGTTGACC CACGCTTTAT TGCGGTTTCT GGATATTGGA TCCCATATAT AAATAGAGGA 
AAACATTTCC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001131089.2
CDS	117..6671
Translation	MADPIMDLFDDPNLFGLDSLTGDSFGRDGPDTIDDALGLGNVLGPLEPITDRVGLPGADVGNNEVKQQTESQVLPDNPALMSATEIMQPIQLPTNQETLNQGNPFMGASNAGAPKIMILKAPAGMTVGACPVTQIQTLTPHQAANGGKVAFAKVLTGTPLRPGVSIVSGNTVLAKMPSTGVAGQVGAVRPVRQLLLQPVRASAAPGSSESNTGIKPAITLTSAPQQGDPKRITLVLQQPSQVGATAQQGQRHVVLGGLPGKIVLQGNQLAALTQAKTPQGQPAKVVTIQLQVQQQPGAAGQTPQKFQIVQQAPGGVAIAPSGQHTHMLNQQGVQRLSVPLKVVLQPQAGSSQGTAQGLSVVKVLNASEVANLTASQTVVKTSTGGGESRKLDSQKKQEKANRIVAEAIARARARGEQNIPRVLNEDELPSVNPEDDDGSRRKRKKKKGETSDRSKDEKPKKVKGSGSLRSRSKGKPSTITPIVGKKRKRNPSSDNSDAEIMASQASPAEDEDSVQKRRSNRQVKRKKYTEDLDIKITDDEDDEEVDVTGPVKTEPVLLPEPMPLPDSEAVPSMQFFVENPSEEDAAIVDKILSMRLAKKELPTGEYVEVEEYFVKYKNYSYLHCEWATIEQLERDKRIHQKLKRFKTKMTQMQHFLQEDEESFNPDYVEVDRILDESHSTDKDNGEPVVYYLVKWCSLPYEDSTWELKEDVDDGKIEEFKRIEARQPNLKRVARPAATSWKKLELSREYQNGNQLREYQLEGVNWLLFNWYNRQNCILADEMGLGKTIQSITFLQEVYNVGIRGPFLVIAPLSTITNWEREFGSWTQMNTIVYHGSLASRQMIQQYEMYCKDSKGRLIPGAYKFDALITTFEMVLSDCPELREIEWRCVIIDEAHRLKNRNCKLLDSLKHMDLEHKVLLTGTPLQNTVEELFSLLHFLEPTQFSSEAEFLKDFGDLKTEEQVQKLQAILKPMMLRRLKEDVEKNLAPKQETIIEVELTNIQKKYYRAILEKNFSFLTKGASQSNTPNLLNTMMELRKCCNHPYLITGAEEKIISEFREATPVVPPDFHVQAMVRSSGKLVLIDKLLPKLRAGGHKVLIFSQMVRCLDILEDYLIQRRYLYERIDGRVRGNMRQAAIDRFSRPDSDRFVFLLCTRAGGLGINLTAADTCIIFDSDWNPQNDLQAQARCHRIGQSKAVKIYRLITRNSYEREMFDKASLKLGLDKAVLQSMSGRDNHLSGPIQQFTKKEIEDLLRKGAYAAIMDEDDEGSKFCEEDIDQILLRRTTTITIESEGKGSTFSKASFVASENRTDISLDDPNFWQKWAKKADLDLDLLSSKNTLVIDTPRIRKQTRHFTNKDDDMVEFSDLESDDDDRPKARRQDRKHGYGRTDCFRVEKHLLVYGWGRWRDILTHGRFKRGMNERDVEKICRAILVYCLLHYRGDENIKSFIWDLITPAENGKTKQLQNHSGLSIPVPRGRKGKKVKSQSSFDIHKADWIHKYNPDTLFQDEGYKKHLKHQCNKVLLRVRMLYFLRQEVIGNQAMKVLSGVEARQIDIWFPQVDQVEVPSRWWDREADKSLLIGVFKHGYEKYNTMRADPALCFLEKVGGPDEQAIAAEHHAQDFSELPDGGDFDRDIEDPEYKPLHAQKDPEDEIDSLMMDEEISVVDGEEATAMPSGMLWPPGSALTARLRRLITAYQRSYKREQLKLEAEERGDKRRKRCEAAFKLKEIARREKQQRWTRREACDFFRVLSSFGVEYDPDTQLYDWQRFRGLARLDKKTDESLLKYFHGFVAMCRQVCRLPPAAGDEPPDPSLFIEPISEERASRALFRLDLLRRVREQVLCHPLLIPRLSLCRPDPELPEWWESGRHDNELLQGAARYGLSRTDLTILQDHAFSFLRCQISYFQSRGISGHSRPSTPTTAPSVPTERQPSHLASLSSSVSCSPAPRRSSSCSSSSHSSNSEDSSDESNGVKVKPNAGLSHARLYDEESRLSLTASPADLTTEDSIQTALETPHSTDWPKDRVLICRIEQVCSAVLTGKWSSPRRLSDPPSDSPDSLPPTPEQQSPAHFTQIRPAPDPKEFTIQIKSEEGLKIKFQKHKFMGNGALDSAPHTQKKKSKKKMDLDMDTRIPVVNQKDGTLLLGEEAPLRCQLPEWLHRNPDFMVDPRFIAVSGYWIPYINRGKHFL

Target ORF information:

RefSeq Version	NM_001137617.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis chromodomain helicase DNA binding protein 8 (chd8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001137617.2

ORF Insert Sequence:

ATGGCTGACC CTATCATGGA CCTCTTTGAT GATCCAAACC TATTTGGCCT TGACTCTCTC 
ACTGGAGATT CCTTTGGACG GGATGGGCCA GACACTATAG ATGATGCTCT AGGCCTGGGT 
AATGTCTTGG GACCTTTGGA GCCGATAACA GACAGAGTTG GCCTGCCTGG TGCTGATGTT 
GGCAATAATG AAGTTAAACA ACAAACAGAG TCACAGGTTC TCCCGGACAA TCCAGCTCTT 
ATGTCAGCTA CTGAGATCAT GCAGCCCATT CAACTTCCCA CAAACCAGGA AACACTGAAC 
CAAGGAAATC CCTTTATGGG TGCCTCAAAT GCTGGAGCCC CAAAAATTAT GATTCTGAAA 
GCTCCTGCTG GAATGACAGT TGGTGCATGT CCTGTAACTC AGATACAAAC TCTGACACCT 
CACCAAGCTG CTAATGGTGG CAAGGTGGCA TTTGCTAAGG TGCTTACAGG AACCCCATTG 
CGTCCTGGGG TCTCAATTGT ATCTGGAAAC ACTGTCCTTG CCAAAATGCC AAGCACTGGA 
GTTGCAGGGC AGGTAGGAGC AGTGAGGCCT GTCCGTCAGC TACTTCTTCA ACCAGTGCGT 
GCCTCCGCAG CCCCAGGAAG CTCCGAGAGT AATACAGGGA TCAAGCCTGC AATCACACTA 
ACGTCAGCAC CTCAACAGGG GGACCCTAAA AGAATTACTC TCGTCCTACA GCAGCCTTCC 
CAAGTTGGTG CAACTGCACA ACAAGGGCAG CGTCATGTTG TGCTGGGTGG GCTTCCAGGA 
AAGATTGTCC TTCAGGGAAA CCAACTGGCA GCTCTCACAC AAGCTAAAAC CCCTCAAGGT 
CAGCCAGCTA AAGTAGTGAC TATCCAGCTG CAAGTCCAGC AGCAGCCTGG GGCTGCAGGT 
CAGACTCCTC AAAAGTTTCA GATTGTACAA CAAGCCCCAG GAGGTGTGGC AATTGCTCCC 
AGTGGGCAAC ATACACACAT GCTTAATCAA CAAGGAGTTC AGAGGCTTTC TGTTCCACTC 
AAAGTTGTTC TGCAACCACA GGCTGGTTCC TCTCAGGGTA CTGCGCAGGG CCTGTCAGTT 
GTGAAAGTTC TAAATGCAAG TGAAGTTGCC AATTTGACTG CAAGTCAGAC TGTGGTTAAA 
ACATCCACTG GAGGTGGTGA ATCTCGTAAG CTGGACAGCC AGAAGAAGCA AGAGAAAGCT 
AACCGTATTG TAGCTGAAGC TATTGCCAGG GCCCGAGCCA GAGGTGAACA GAATATTCCT 
CGGGTCCTGA ATGAGGATGA GCTTCCCAGT GTGAATCCTG AAGATGATGA TGGAAGCAGG 
CGGAAAAGGA AGAAGAAGAA AGGAGAGACT AGTGATAGGT CCAAGGATGA GAAACCTAAG 
AAGGTCAAAG GTAGTGGAAG CTTAAGGTCT AGGAGCAAAG GCAAACCAAG TACTATCACT 
CCTATTGTGG GTAAGAAAAG GAAGAGAAAC CCATCTTCCG ATAATTCTGA TGCTGAGATC 
ATGGCATCAC AGGCTTCTCC TGCAGAGGAT GAAGACAGTG TCCAGAAGAG ACGTTCAAAT 
CGCCAGGTGA AGCGCAAGAA GTATACAGAG GATTTGGATA TTAAAATAAC AGATGATGAA 
GATGATGAGG AGGTAGATGT GACGGGACCT GTGAAAACAG AACCTGTGCT GCTTCCTGAG 
CCCATGCCTT TACCTGACAG TGAGGCAGTC CCTTCAATGC AGTTCTTCGT GGAGAACCCA 
AGTGAAGAAG ATGCTGCTAT TGTTGACAAA ATACTCTCTA TGCGCCTAGC TAAAAAAGAG 
CTGCCCACTG GAGAGTATGT TGAAGTTGAA GAGTACTTTG TCAAATATAA AAATTATTCC 
TACCTACACT GTGAATGGGC CACAATTGAG CAACTGGAAA GGGACAAGCG TATTCATCAG 
AAACTGAAAA GATTTAAGAC AAAGATGACC CAGATGCAGC ATTTCTTGCA AGAAGATGAA 
GAATCTTTCA ATCCAGACTA TGTAGAGGTA GATCGAATCC TAGACGAATC TCACAGCACT 
GACAAGGACA ATGGTGAACC GGTGGTGTAT TACCTAGTAA AATGGTGCTC TTTACCTTAT 
GAAGATAGCA CATGGGAGCT GAAAGAAGAT GTTGATGATG GAAAGATAGA GGAATTCAAA 
CGAATAGAGG CGAGGCAGCC AAATCTCAAA CGCGTGGCCC GCCCAGCTGC TACATCATGG 
AAGAAGCTGG AATTGTCTCG GGAGTATCAG AATGGCAACC AGTTGAGAGA ATACCAGCTG 
GAAGGTGTTA ATTGGCTTCT CTTTAACTGG TACAATAGGC AAAACTGCAT TTTGGCAGAT 
GAAATGGGTT TGGGGAAGAC CATCCAATCT ATCACATTCT TACAAGAAGT ATATAATGTG 
GGAATACGGG GACCTTTTTT AGTAATTGCT CCACTTTCCA CCATCACGAA CTGGGAGAGA 
GAGTTTGGCA GTTGGACTCA AATGAATACT ATTGTGTATC ATGGCAGCCT TGCTAGTCGC 
CAAATGATCC AGCAATATGA GATGTACTGT AAGGACTCCA AGGGTCGTCT TATTCCTGGT 
GCGTACAAGT TTGATGCTCT TATTACAACT TTTGAAATGG TGCTGTCTGA TTGCCCAGAG 
CTGCGTGAAA TTGAGTGGCG CTGTGTCATA ATTGATGAGG CTCATCGGCT CAAAAACAGA 
AACTGCAAAC TACTGGACAG CCTAAAGCAC ATGGACTTGG AGCACAAAGT CCTTTTAACA 
GGCACTCCAT TACAGAACAC CGTGGAAGAG TTGTTCAGTC TTTTGCATTT CCTGGAGCCA 
ACACAATTTT CCTCTGAAGC AGAGTTTCTT AAGGACTTTG GAGACCTGAA GACAGAGGAA 
CAGGTGCAGA AATTACAGGC CATCTTAAAA CCTATGATGT TGAGGCGATT AAAGGAAGAT 
GTTGAGAAAA ACCTGGCCCC GAAACAAGAA ACTATAATTG AGGTTGAGCT TACTAACATC 
CAGAAGAAGT ATTACCGAGC AATACTGGAG AAGAATTTTT CTTTTCTGAC CAAAGGAGCT 
AGTCAATCCA ACACACCCAA CTTGCTCAAT ACCATGATGG AACTACGCAA ATGCTGCAAC 
CACCCATACC TCATCACTGG AGCAGAAGAA AAGATCATAT CTGAGTTCCG AGAAGCAACT 
CCAGTAGTTC CCCCTGACTT TCACGTCCAA GCAATGGTAC GCTCCTCTGG CAAACTGGTG 
CTAATAGATA AGCTGCTCCC TAAACTAAGG GCTGGAGGCC ACAAAGTTCT CATCTTTTCT 
CAGATGGTAC GGTGCCTGGA CATCCTGGAA GACTATCTCA TTCAGAGAAG GTACCTTTAT 
GAGCGTATTG ATGGACGTGT CCGTGGCAAC ATGCGTCAGG CAGCCATTGA TAGATTCAGC 
CGCCCAGATT CTGACCGATT TGTGTTTCTA CTCTGTACAC GAGCTGGTGG GCTTGGAATT 
AACTTGACAG CTGCTGATAC TTGTATTATA TTTGACTCGG ACTGGAACCC TCAAAATGAC 
CTTCAGGCTC AAGCTCGATG TCATCGTATT GGGCAAAGCA AAGCCGTTAA AATTTACAGA 
CTCATCACTC GCAACTCCTA TGAGAGAGAG ATGTTTGATA AGGCCAGTCT CAAACTAGGA 
CTAGACAAAG CTGTATTGCA GTCTATGAGT GGTCGGGATA ATCACCTTAG TGGGCCTATT 
CAGCAATTCA CAAAGAAAGA AATTGAAGAC TTGCTAAGGA AAGGAGCTTA TGCTGCAATC 
ATGGATGAAG ATGACGAAGG CTCTAAATTC TGTGAAGAAG ATATTGACCA AATTTTACTG 
AGGCGTACCA CGACTATTAC CATAGAGTCA GAGGGAAAGG GCTCAACCTT CTCCAAGGCA 
AGTTTTGTTG CTTCTGAGAA CAGGACTGAT ATATCCCTAG ATGATCCTAA TTTCTGGCAA 
AAGTGGGCCA AGAAAGCAGA CTTAGACTTG GATCTTCTTA GCAGCAAGAA TACACTGGTT 
ATTGATACCC CACGTATCAG GAAGCAAACA CGGCACTTTA CTAACAAAGA TGATGACATG 
GTTGAGTTCT CAGATTTGGA GAGTGATGAT GATGATCGCC CCAAAGCTCG GCGTCAAGAC 
CGCAAGCATG GATATGGACG TACAGATTGC TTCCGTGTGG AGAAGCATTT GCTTGTTTAT 
GGATGGGGTC GCTGGCGAGA TATCCTTACT CATGGTCGTT TCAAGCGTGG TATGAATGAG 
CGTGACGTGG AGAAAATATG TAGAGCCATA CTGGTTTATT GTCTTTTGCA CTACCGGGGA 
GATGAAAACA TAAAGAGCTT TATCTGGGAT CTAATTACAC CAGCTGAGAA TGGCAAAACT 
AAGCAGCTAC AGAACCACTC TGGATTGTCT ATTCCTGTCC CCCGTGGTCG TAAGGGGAAA 
AAAGTGAAAT CCCAAAGCAG CTTTGACATA CATAAAGCCG ATTGGATACA TAAGTACAAC 
CCAGATACCT TGTTCCAAGA TGAAGGGTAC AAGAAACATC TCAAGCATCA ATGTAACAAG 
GTGCTGCTTC GCGTTCGTAT GTTATATTTT CTTCGTCAGG AAGTTATTGG AAACCAGGCA 
ATGAAAGTTT TAAGTGGTGT AGAGGCCAGG CAAATTGACA TCTGGTTCCC ACAGGTGGAC 
CAGGTTGAAG TTCCCTCGCG CTGGTGGGAC AGAGAAGCTG ATAAATCTCT TCTTATCGGG 
GTCTTTAAAC ATGGTTATGA GAAGTATAAC ACCATGCGAG CTGACCCTGC ACTCTGTTTT 
TTGGAGAAGG TGGGGGGACC AGATGAACAG GCTATTGCAG CTGAGCACCA TGCTCAAGAC 
TTTTCTGAAC TCCCTGATGG TGGGGACTTT GATCGAGACA TAGAAGATCC AGAATACAAG 
CCACTTCATG CACAGAAAGA TCCTGAGGAT GAGATCGACT CTTTAATGAT GGATGAAGAA 
ATCTCTGTTG TTGACGGTGA AGAAGCAACA GCAATGCCCA GTGGTATGTT ATGGCCACCT 
GGCTCTGCTC TCACAGCTCG TCTACGGCGT CTAATTACTG CCTACCAACG CAGCTACAAA 
AGAGAACAAC TAAAATTGGA GGCTGAAGAG CGTGGGGACA AACGTAGAAA ACGATGTGAG 
GCAGCCTTTA AACTGAAAGA AATTGCCCGT AGGGAGAAAC AGCAAAGGTG GACCCGAAGG 
GAGGCATGTG ACTTTTTTCG TGTGCTGTCC AGCTTTGGTG TCGAGTATGA CCCCGATACT 
CAACTGTATG ACTGGCAGAG GTTCCGTGGT CTGGCAAGAC TGGACAAGAA GACAGATGAG 
TCACTGCTCA AATACTTCCA TGGATTTGTG GCAATGTGCC GCCAGGTTTG CCGCTTACCC 
CCTGCTGCCG GAGACGAGCC ACCAGATCCA TCTCTCTTCA TTGAGCCAAT TTCAGAAGAG 
CGAGCATCTC GCGCCCTGTT TCGCTTGGAT CTTCTTCGTA GGGTGCGTGA GCAAGTACTT 
TGTCATCCTT TGCTTATTCC TCGTTTGTCA CTGTGCCGGC CAGACCCAGA ACTCCCAGAA 
TGGTGGGAGA GTGGACGTCA TGACAATGAG CTTCTTCAGG GAGCAGCACG TTATGGTCTG 
AGCCGTACAG ACCTCACTAT ACTGCAGGAC CATGCATTTT CTTTTTTGCG GTGTCAGATC 
AGTTATTTCC AGTCAAGGGG AATATCTGGC CATTCTCGCC CTTCTACTCC AACTACTGCC 
CCTTCAGTGC CTACTGAACG TCAGCCTTCT CATCTAGCCT CCCTTTCCTC ATCTGTCAGC 
TGTTCTCCTG CTCCACGCAG ATCCTCTTCC TGCTCATCAT CATCTCACTC ATCAAACTCT 
GAGGATTCTT CAGATGAAAG CAATGGTGTA AAAGTAAAAC CTAATGCAGG TCTTTCGCAC 
GCTCGACTGT ATGATGAGGA GAGCCGGCTT TCTCTTACTG CCTCCCCTGC AGATCTCACT 
ACAGAGGACA GTATTCAGAC AGCGCTGGAG ACCCCGCACA GTACAGACTG GCCAAAGGAC 
CGTGTCCTTA TCTGCAGAAT TGAGCAAGTA TGCAGTGCCG TTCTTACTGG CAAATGGTCC 
TCACCACGAC GACTGTCAGA TCCACCAAGT GACAGCCCTG ATAGCTTGCC TCCAACCCCA 
GAGCAGCAGA GTCCAGCCCA CTTCACACAG ATAAGACCTG CACCGGACCC CAAAGAGTTT 
ACCATTCAAA TAAAAAGTGA AGAAGGCCTG AAGATTAAAT TTCAGAAGCA CAAATTTATG 
GGTAATGGAG CTCTTGATTC TGCACCTCAT ACCCAGAAGA AAAAGAGCAA GAAAAAGATG 
GATCTTGACA TGGACACACG AATTCCCGTT GTAAACCAGA AGGATGGGAC CTTGTTGTTA 
GGGGAAGAAG CCCCTTTACG CTGCCAGCTG CCCGAGTGGT TACACAGAAA TCCTGACTTC 
ATGGTTGACC CACGCTTTAT TGCGGTTTCT GGATATTGGA TCCCATATAT AAATAGAGGA 
AAACATTTCC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chd8 ( XM_012957178.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012812632.1 Xenopus tropicalis chromodomain helicase DNA binding protein 8 (chd8), transcript variant X2, mRNA.

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CloneID	OXa32500
Clone ID Related Accession (Same CDS sequence)	XM_012957172.3 , XM_012957178.3 , XM_031896082.1 , XM_012957178.2 , XM_012957172.2 , XM_012957182.2 , XM_012957182.3
Accession Version	XM_012957178.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7008bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	chromodomain-helicase-DNA-binding protein 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957178.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACC CTATCATGGA CCTCTTTGAT GATCCAAACC TATTTGGCCT TGACTCTCTC 
ACTGGAGATT CCTTTGGACG GGATGGGCCA GACACTATAG ATGATGCTCT AGGCCTGGGT 
AATGTCTTGG GACCTTTGGA GCCGATAACA GACAGAGTTG GCCTGCCTGG TGCTGATGTT 
GGCAATAATG AAGTTAAACA ACAAACAGAG TCACAGGTTC TCCCGGACAA TCCAGCTCTT 
ATGTCAGCTA CTGAGATCAT GCAGCCCATT CAACTTCCCA CAAACCAGGA AACACTGAAC 
CAAGGAAATC CCTTTATGGG TGCCTCAAAT GCTGGAGCCC CAAAAATTAT GATTCTGAAA 
GCTCCTGCTG GAATGACAGT TGGTGCATGT CCTGTAACTC AGATACAAAC TCTGACACCT 
CACCAAGCTG CTAATGGTGG CAAGGTGGCA TTTGCTAAGG TGCTTACAGG AACCCCATTG 
CGTCCTGGGG TCTCAATTGT ATCTGGAAAC ACTGTCCTTG CCAAAATGCC AAGCACTGGA 
GTTGCAGGGC AGGTAGGAGC AGTGAGGCCT GTCCGTCAGC TACTTCTTCA ACCAGTGCGT 
GCCTCCGCAG CCCCAGGAAG CTCCGAGAGT AATACAGGGA TCAAGCCTGC AATCACACTA 
ACGTCAGCAC CTCAACAGGG GGACCCTAAA AGAATTACTC TCGTCCTACA GCAGCCTTCC 
CAAGTTGGTG CAACTGCACA ACAAGGGCAG CGTCATGTTG TGCTGGGTGG GCTTCCAGGA 
AAGATTGTCC TTCAGGGAAA CCAACTGGCA GCTCTCACAC AAGCTAAAAC CCCTCAAGGT 
CAGCCAGCTA AAGTAGTGAC TATCCAGCTG CAAGTCCAGC AGCAGCCTGG GGCTGCAGGT 
CAGACTCCTC AAAAGTTTCA GATTGTACAA CAAGCCCCAG GAGGTGTGGC AATTGCTCCC 
AGTGGGCAAC ATACACACAT GCTTAATCAA CAAGGAGTTC AGAGGCTTTC TGTTCCACTC 
AAAGTTGTTC TGCAACCACA GGCTGGTTCC TCTCAGGGTA CTGCGCAGGG CCTGTCAGTT 
GTGAAAGTTC TAAATGCAAG TGAAGTTGCC AATTTGACTG CAAGTCAGAC TGTGGTTAAA 
ACATCCACTG GAGGTGGTGA ATCTCGTAAG CTGGACAGCC AGAAGAAGCA AGAGAAAGCT 
AACCGTATTG TAGCTGAAGC TATTGCCAGG GCCCGAGCCA GAGGTGAACA GAATATTCCT 
CGGGTCCTGA ATGAGGATGA GCTTCCCAGT GTGAATCCTG AAGATGATGA TGGAAGCAGG 
CGGAAAAGGA AGAAGAAGAA AGGAGAGACT AGTGATAGGT CCAAGGATGA GAAACCTAAG 
AAGGTCAAAG GTAGTGGAAG CTTAAGGTCT AGGAGCAAAG GCAAACCAAG TACTATCACT 
CCTATTGTGG GTAAGAAAAG GAAGAGAAAC CCATCTTCCG ATAATTCTGA TGCTGAGATC 
ATGGCATCAC AGGCTTCTCC TGCAGAGGAT GAAGACAGTG TCCAGAAGAG ACGTTCAAAT 
CGCCAGGTGA AGCGCAAGAA GTATACAGAG GATTTGGATA TTAAAATAAC AGATGATGAA 
GATGATGAGG AGGTAGATGT GACGGGACCT GTGAAAACAG AACCTGTGCT GCTTCCTGAG 
CCCATGCCTT TACCTGACAG TGAGGCAGTC CCTTCAATGC AGTTCTTCGT GGAGAACCCA 
AGTGAAGAAG ATGCTGCTAT TGTTGACAAA ATACTCTCTA TGCGCCTAGC TAAAAAAGAG 
CTGCCCACTG GAGAGTATGT TGAAGTTGAA GAGTACTTTG TCAAATATAA AAATTATTCC 
TACCTACACT GTGAATGGGC CACAATTGAG CAACTGGAAA GGGACAAGCG TATTCATCAG 
AAACTGAAAA GATTTAAGAC AAAGATGACC CAGATGCAGC ATTTCTTGCA AGAAGATGAA 
GAATCTTTCA ATCCAGACTA TGTAGAGGTA GATCGAATCC TAGACGAATC TCACAGCACT 
GACAAGGACA ATGGTGAACC GGTGGTGTAT TACCTAGTAA AATGGTGCTC TTTACCTTAT 
GAAGATAGCA CATGGGAGCT GAAAGAAGAT GTTGATGATG GAAAGATAGA GGAATTCAAA 
CGAATAGAGG CGAGGCAGCC AAATCTCAAA CGCGTGGCCC GCCCAGCTGC TACATCATGG 
AAGAAGCTGG AATTGTCTCG GGAGTATCAG AATGGCAACC AGTTGAGAGA ATACCAGCTG 
GAAGGTGTTA ATTGGCTTCT CTTTAACTGG TACAATAGGC AAAACTGCAT TTTGGCAGAT 
GAAATGGGTT TGGGGAAGAC CATCCAATCT ATCACATTCT TACAAGAAGT ATATAATGTG 
GGAATACGGG GACCTTTTTT AGTAATTGCT CCACTTTCCA CCATCACGAA CTGGGAGAGA 
GAGTTTGGCA GTTGGACTCA AATGAATACT ATTGTGTATC ATGGCAGCCT TGCTAGTCGC 
CAAATGATCC AGCAATATGA GATGTACTGT AAGGACTCCA AGGGTCGTCT TATTCCTGGT 
GCGTACAAGT TTGATGCTCT TATTACAACT TTTGAAATGG TGCTGTCTGA TTGCCCAGAG 
CTGCGTGAAA TTGAGTGGCG CTGTGTCATA ATTGATGAGG CTCATCGGCT CAAAAACAGA 
AACTGCAAAC TACTGGACAG CCTAAAGCAC ATGGACTTGG AGCACAAAGT CCTTTTAACA 
GGCACTCCAT TACAGAACAC CGTGGAAGAG TTGTTCAGTC TTTTGCATTT CCTGGAGCCA 
ACACAATTTT CCTCTGAAGC AGAGTTTCTT AAGGACTTTG GAGACCTGAA GACAGAGGAA 
CAGGTGCAGA AATTACAGGC CATCTTAAAA CCTATGATGT TGAGGCGATT AAAGGAAGAT 
GTTGAGAAAA ACCTGGCCCC GAAACAAGAA ACTATAATTG AGGTTGAGCT TACTAACATC 
CAGAAGAAGT ATTACCGAGC AATACTGGAG AAGAATTTTT CTTTTCTGAC CAAAGGAGCT 
AGTCAATCCA ACACACCCAA CTTGCTCAAT ACCATGATGG AACTACGCAA ATGCTGCAAC 
CACCCATACC TCATCACTGG AGCAGAAGAA AAGATCATAT CTGAGTTCCG AGAAGCAACT 
CCAGTAGTTC CCCCTGACTT TCACGTCCAA GCAATGGTAC GCTCCTCTGG CAAACTGGTG 
CTAATAGATA AGCTGCTCCC TAAACTAAGG GCTGGAGGCC ACAAAGTTCT CATCTTTTCT 
CAGATGGTAC GGTGCCTGGA CATCCTGGAA GACTATCTCA TTCAGAGAAG GTACCTTTAT 
GAGCGTATTG ATGGACGTGT CCGTGGCAAC ATGCGTCAGG CAGCCATTGA TAGATTCAGC 
CGCCCAGATT CTGACCGATT TGTGTTTCTA CTCTGTACAC GAGCTGGTGG GCTTGGAATT 
AACTTGACAG CTGCTGATAC TTGTATTATA TTTGACTCGG ACTGGAACCC TCAAAATGAC 
CTTCAGGCTC AAGCTCGATG TCATCGTATT GGGCAAAGCA AAGCCGTTAA AATTTACAGA 
CTCATCACTC GCAACTCCTA TGAGAGAGAG ATGTTTGATA AGGCCAGTCT CAAACTAGGA 
CTAGACAAAG CTGTATTGCA GTCTATGAGT GGTCGGGATA ATCACCTTAG TGGGCCTATT 
CAGCAATTCA CAAAGAAAGA AATTGAAGAC TTGCTAAGGA AAGGAGCTTA TGCTGCAATC 
ATGGATGAAG ATGACGAAGG CTCTAAATTC TGTGAAGAAG ATATTGACCA AATTTTACTG 
AGGCGTACCA CGACTATTAC CATAGAGTCA GAGGGAAAGG GCTCAACCTT CTCCAAGGCA 
AGTTTTGTTG CTTCTGAGAA CAGGACTGAT ATATCCCTAG ATGATCCTAA TTTCTGGCAA 
AAGTGGGCCA AGAAAGCAGA CTTAGACTTG GATCTTCTTA GCAGCAAGAA TACACTGGTT 
ATTGATACCC CACGTATCAG GAAGCAAACA CGGCACTTTA CTAACAAAGA TGATGACATG 
GTTGAGTTCT CAGATTTGGA GAGTGATGAT GATGATCGCC CCAAAGCTCG GCGTCAAGAC 
CGCAAGCATG GATATGGACG TACAGATTGC TTCCGTGTGG AGAAGCATTT GCTTGTTTAT 
GGATGGGGTC GCTGGCGAGA TATCCTTACT CATGGTCGTT TCAAGCGTGG TATGAATGAG 
CGTGACGTGG AGAAAATATG TAGAGCCATA CTGGTTTATT GTCTTTTGCA CTACCGGGGA 
GATGAAAACA TAAAGAGCTT TATCTGGGAT CTAATTACAC CAGCTGAGAA TGGCAAAACT 
AAGCAGCTAC AGAACCACTC TGGATTGTCT ATTCCTGTCC CCCGTGGTCG TAAGGGGAAA 
AAAGTGAAAT CCCAAAGCAG CTTTGACATA CATAAAGCCG ATTGGATACA TAAGTACAAC 
CCAGATACCT TGTTCCAAGA TGAAGGGTAC AAGAAACATC TCAAGCATCA ATGTAACAAG 
GTGCTGCTTC GCGTTCGTAT GTTATATTTT CTTCGTCAGG AAGTTATTGG AAACCAGGCA 
ATGAAAGTTT TAAGTGGTGT AGAGGCCAGG CAAATTGACA TCTGGTTCCC ACAGGTGGAC 
CAGGTTGAAG TTCCCTCACG CTGGTGGGAC AGAGAAGCTG ATAAATCTCT TCTTATCGGG 
GTCTTTAAAC ATGGTTATGA GAAGTATAAC ACCATGCGAG CTGACCCTGC ACTCTGTTTT 
TTGGAGAAGG TGGGGGGACC AGATGAACAG GCTATTGCAG CTGAGCACCA TGCTCAAGAC 
TTTTCTGAAC TCCCTGATGG TGGGGACTTT GATCGAGACA TAGAAGATCC AGAATACAAG 
CCACTTCATG CACAGAAAGA TCCTGAGGAT GAGATCGACT CTTTAATGAT GGATGAAGAA 
ATCTCTGTTG TTGACGGTGA AGAAGCAACA GCAATGCCCA GTGGTATGTT ATGGCCACCT 
GGCTCTGCTC TCACAGCTCG TCTACGGCGT CTAATTACTG CCTACCAACG CAGCTACAAA 
AGAGAACAAC TAAAATTGGA GGCTGAAGAG CGTGGGGACA AACGTAGAAA ACGATGTGAG 
GCAGCCTTTA AACTGAAAGA AATTGCCCGT AGGGAGAAAC AGCAAAGGTG GACCCGAAGG 
GAGGCATGTG ACTTTTTTCG TGTGCTGTCC AGCTTTGGTG TCGAGTATGA CCCCGATACT 
CAACTGTATG ACTGGCAGAG GTTCCGTGGT CTGGCAAGAC TGGACAAGAA GACAGATGAG 
TCACTGCTCA AATACTTCCA TGGATTTGTG GCAATGTGCC GCCAGGTTTG CCGCTTACCC 
CCTGCTGCCG GAGACGAGCC ACCAGATCCA TCTCTCTTCA TTGAGCCAAT TTCAGAAGAG 
CGAGCATCTC GCGCCCTGTT TCGCTTGGAT CTTCTTCGTA GGGTGCGTGA GCAAGTACTT 
TGTCATCCTT TGCTTATTCC TCGTTTGTCA CTGTGCCGGC CAGACCCAGA ACTCCCAGAA 
TGGTGGGAGA GTGGACGTCA TGACAATGAG CTTCTTCAGG GAGCAGCACG TTATGGTCTG 
AGCCGTACAG ACCTCACTAT ACTGCAGGAC CATGCATTTT CTTTTTTGCG GTGTCAGATC 
AGTTATTTCC AGTCAAGGGG AATATCTGGC CATTCTCGCC CTTCTACTCC AACTACTGCC 
CCTTCAGTGC CTACTGAACG TCAGCCTTCT CATCTAGCCT CCCTTTCCTC ATCTGTCAGC 
TGTTCTCCTG CTCCACGCAG ATCCTCTTCC TGCTCATCAT CATCTCACTC ATCAAACTCT 
GAGGATTCTT CAGATGAAAG CAATGGTGTA AAAGTAAAAC CTAATGCAGG TCTTTCGCAC 
GCTCGACTGT ATGATGAGGA GAGCCGGCTT TCTCTTACTG CCTCCCCTGC AGATCTCACT 
ACAGAGGACA GTATTCAGAC AGCGCTGGAG ACCCCGCACA GTACAGACTG GCCAAAGGAC 
CGTGTCCTTA TCTGCAGAAT TGAGCAAGTA TGCAGTGCCG TTCTTACTGG CAAATGGTCC 
TCACCACGAC GACTGTCAGA TCCACCAAGT GACAGCCCTG ATAGCTTGCC TCCAACCCCA 
GAGCAGCAGA GTCCAGCCCA CTTCACACAG ATAAGACCTG CACCGGACCC CAAAGAGTTT 
ACCATTCAAA TAAAAAGTGA AGAAGGCCTG AAGATTAAAT TTCAGAAGCA CAAATTTATG 
GGTAATGGAG CTCTTGATTC TGCACCTCAT ACCCAGAAGA AAAAGAGCAA GAAAAAGATG 
GATCTTGACA TGGACACACG AATTCCCGTT GTAAACCAGA AGGATGGGAC CTTGTTGTTA 
GGGGAAGAAG CCCCTTTACG CTGCCAGCTG CCCGAGTGGT TACACAGAAA TCCTGACTTC 
ATGGTTGACC CACGCTTTAT TGCGTTTATG GAAGACCGCA GGAAAAGAAA AAAACATCGA 
AGCAAAAAGT CCAAATCCTC CTCCCAGATT GGAAACGTAC AGAATCCCCA CTCCCACAAT 
GGAAAGAAGT CTTCCCACAC AGAAAACTCT TTCGAGACGC AACACCTTCC TACTGCAGCC 
AAAAAAAGGA AGAAACGCCC TCCTTCAAAA ATAATAATGC CATCTGCACT ACATGGAGCT 
GGTGATGTTA CACATTCCTT CTCAGACCTT CAACCTGACC ATTCTGGTCA CCCCATGCGG 
TCAGTGTATG GACCATCAGA TGGAGGGCTG TCCCATCAAG CAGGACTCAG CGGGTTGGGA 
TTTACCCGCT TTCATTCATC GCAGACATCT TCAGTGGGCA GAATCCATGG CACTACCTTT 
GATGAGTTAC AAAGTTTTGA GCCCCCACAA CAACATTTTT CTCTGCTTGA TGATCCTATG 
ATGCCAGCAA ATTCCTCCTC CAGCGGTGAT GAACTGGACA GACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012812632.1
CDS	291..7298
Translation	MADPIMDLFDDPNLFGLDSLTGDSFGRDGPDTIDDALGLGNVLGPLEPITDRVGLPGADVGNNEVKQQTESQVLPDNPALMSATEIMQPIQLPTNQETLNQGNPFMGASNAGAPKIMILKAPAGMTVGACPVTQIQTLTPHQAANGGKVAFAKVLTGTPLRPGVSIVSGNTVLAKMPSTGVAGQVGAVRPVRQLLLQPVRASAAPGSSESNTGIKPAITLTSAPQQGDPKRITLVLQQPSQVGATAQQGQRHVVLGGLPGKIVLQGNQLAALTQAKTPQGQPAKVVTIQLQVQQQPGAAGQTPQKFQIVQQAPGGVAIAPSGQHTHMLNQQGVQRLSVPLKVVLQPQAGSSQGTAQGLSVVKVLNASEVANLTASQTVVKTSTGGGESRKLDSQKKQEKANRIVAEAIARARARGEQNIPRVLNEDELPSVNPEDDDGSRRKRKKKKGETSDRSKDEKPKKVKGSGSLRSRSKGKPSTITPIVGKKRKRNPSSDNSDAEIMASQASPAEDEDSVQKRRSNRQVKRKKYTEDLDIKITDDEDDEEVDVTGPVKTEPVLLPEPMPLPDSEAVPSMQFFVENPSEEDAAIVDKILSMRLAKKELPTGEYVEVEEYFVKYKNYSYLHCEWATIEQLERDKRIHQKLKRFKTKMTQMQHFLQEDEESFNPDYVEVDRILDESHSTDKDNGEPVVYYLVKWCSLPYEDSTWELKEDVDDGKIEEFKRIEARQPNLKRVARPAATSWKKLELSREYQNGNQLREYQLEGVNWLLFNWYNRQNCILADEMGLGKTIQSITFLQEVYNVGIRGPFLVIAPLSTITNWEREFGSWTQMNTIVYHGSLASRQMIQQYEMYCKDSKGRLIPGAYKFDALITTFEMVLSDCPELREIEWRCVIIDEAHRLKNRNCKLLDSLKHMDLEHKVLLTGTPLQNTVEELFSLLHFLEPTQFSSEAEFLKDFGDLKTEEQVQKLQAILKPMMLRRLKEDVEKNLAPKQETIIEVELTNIQKKYYRAILEKNFSFLTKGASQSNTPNLLNTMMELRKCCNHPYLITGAEEKIISEFREATPVVPPDFHVQAMVRSSGKLVLIDKLLPKLRAGGHKVLIFSQMVRCLDILEDYLIQRRYLYERIDGRVRGNMRQAAIDRFSRPDSDRFVFLLCTRAGGLGINLTAADTCIIFDSDWNPQNDLQAQARCHRIGQSKAVKIYRLITRNSYEREMFDKASLKLGLDKAVLQSMSGRDNHLSGPIQQFTKKEIEDLLRKGAYAAIMDEDDEGSKFCEEDIDQILLRRTTTITIESEGKGSTFSKASFVASENRTDISLDDPNFWQKWAKKADLDLDLLSSKNTLVIDTPRIRKQTRHFTNKDDDMVEFSDLESDDDDRPKARRQDRKHGYGRTDCFRVEKHLLVYGWGRWRDILTHGRFKRGMNERDVEKICRAILVYCLLHYRGDENIKSFIWDLITPAENGKTKQLQNHSGLSIPVPRGRKGKKVKSQSSFDIHKADWIHKYNPDTLFQDEGYKKHLKHQCNKVLLRVRMLYFLRQEVIGNQAMKVLSGVEARQIDIWFPQVDQVEVPSRWWDREADKSLLIGVFKHGYEKYNTMRADPALCFLEKVGGPDEQAIAAEHHAQDFSELPDGGDFDRDIEDPEYKPLHAQKDPEDEIDSLMMDEEISVVDGEEATAMPSGMLWPPGSALTARLRRLITAYQRSYKREQLKLEAEERGDKRRKRCEAAFKLKEIARREKQQRWTRREACDFFRVLSSFGVEYDPDTQLYDWQRFRGLARLDKKTDESLLKYFHGFVAMCRQVCRLPPAAGDEPPDPSLFIEPISEERASRALFRLDLLRRVREQVLCHPLLIPRLSLCRPDPELPEWWESGRHDNELLQGAARYGLSRTDLTILQDHAFSFLRCQISYFQSRGISGHSRPSTPTTAPSVPTERQPSHLASLSSSVSCSPAPRRSSSCSSSSHSSNSEDSSDESNGVKVKPNAGLSHARLYDEESRLSLTASPADLTTEDSIQTALETPHSTDWPKDRVLICRIEQVCSAVLTGKWSSPRRLSDPPSDSPDSLPPTPEQQSPAHFTQIRPAPDPKEFTIQIKSEEGLKIKFQKHKFMGNGALDSAPHTQKKKSKKKMDLDMDTRIPVVNQKDGTLLLGEEAPLRCQLPEWLHRNPDFMVDPRFIAFMEDRRKRKKHRSKKSKSSSQIGNVQNPHSHNGKKSSHTENSFETQHLPTAAKKRKKRPPSKIIMPSALHGAGDVTHSFSDLQPDHSGHPMRSVYGPSDGGLSHQAGLSGLGFTRFHSSQTSSVGRIHGTTFDELQSFEPPQQHFSLLDDPMMPANSSSSGDELDRP

Target ORF information:

RefSeq Version	XM_012957178.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis chromodomain helicase DNA binding protein 8 (chd8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957178.2

ORF Insert Sequence:

ATGGCTGACC CTATCATGGA CCTCTTTGAT GATCCAAACC TATTTGGCCT TGACTCTCTC 
ACTGGAGATT CCTTTGGACG GGATGGGCCA GACACTATAG ATGATGCTCT AGGCCTGGGT 
AATGTCTTGG GACCTTTGGA GCCGATAACA GACAGAGTTG GCCTGCCTGG TGCTGATGTT 
GGCAATAATG AAGTTAAACA ACAAACAGAG TCACAGGTTC TCCCGGACAA TCCAGCTCTT 
ATGTCAGCTA CTGAGATCAT GCAGCCCATT CAACTTCCCA CAAACCAGGA AACACTGAAC 
CAAGGAAATC CCTTTATGGG TGCCTCAAAT GCTGGAGCCC CAAAAATTAT GATTCTGAAA 
GCTCCTGCTG GAATGACAGT TGGTGCATGT CCTGTAACTC AGATACAAAC TCTGACACCT 
CACCAAGCTG CTAATGGTGG CAAGGTGGCA TTTGCTAAGG TGCTTACAGG AACCCCATTG 
CGTCCTGGGG TCTCAATTGT ATCTGGAAAC ACTGTCCTTG CCAAAATGCC AAGCACTGGA 
GTTGCAGGGC AGGTAGGAGC AGTGAGGCCT GTCCGTCAGC TACTTCTTCA ACCAGTGCGT 
GCCTCCGCAG CCCCAGGAAG CTCCGAGAGT AATACAGGGA TCAAGCCTGC AATCACACTA 
ACGTCAGCAC CTCAACAGGG GGACCCTAAA AGAATTACTC TCGTCCTACA GCAGCCTTCC 
CAAGTTGGTG CAACTGCACA ACAAGGGCAG CGTCATGTTG TGCTGGGTGG GCTTCCAGGA 
AAGATTGTCC TTCAGGGAAA CCAACTGGCA GCTCTCACAC AAGCTAAAAC CCCTCAAGGT 
CAGCCAGCTA AAGTAGTGAC TATCCAGCTG CAAGTCCAGC AGCAGCCTGG GGCTGCAGGT 
CAGACTCCTC AAAAGTTTCA GATTGTACAA CAAGCCCCAG GAGGTGTGGC AATTGCTCCC 
AGTGGGCAAC ATACACACAT GCTTAATCAA CAAGGAGTTC AGAGGCTTTC TGTTCCACTC 
AAAGTTGTTC TGCAACCACA GGCTGGTTCC TCTCAGGGTA CTGCGCAGGG CCTGTCAGTT 
GTGAAAGTTC TAAATGCAAG TGAAGTTGCC AATTTGACTG CAAGTCAGAC TGTGGTTAAA 
ACATCCACTG GAGGTGGTGA ATCTCGTAAG CTGGACAGCC AGAAGAAGCA AGAGAAAGCT 
AACCGTATTG TAGCTGAAGC TATTGCCAGG GCCCGAGCCA GAGGTGAACA GAATATTCCT 
CGGGTCCTGA ATGAGGATGA GCTTCCCAGT GTGAATCCTG AAGATGATGA TGGAAGCAGG 
CGGAAAAGGA AGAAGAAGAA AGGAGAGACT AGTGATAGGT CCAAGGATGA GAAACCTAAG 
AAGGTCAAAG GTAGTGGAAG CTTAAGGTCT AGGAGCAAAG GCAAACCAAG TACTATCACT 
CCTATTGTGG GTAAGAAAAG GAAGAGAAAC CCATCTTCCG ATAATTCTGA TGCTGAGATC 
ATGGCATCAC AGGCTTCTCC TGCAGAGGAT GAAGACAGTG TCCAGAAGAG ACGTTCAAAT 
CGCCAGGTGA AGCGCAAGAA GTATACAGAG GATTTGGATA TTAAAATAAC AGATGATGAA 
GATGATGAGG AGGTAGATGT GACGGGACCT GTGAAAACAG AACCTGTGCT GCTTCCTGAG 
CCCATGCCTT TACCTGACAG TGAGGCAGTC CCTTCAATGC AGTTCTTCGT GGAGAACCCA 
AGTGAAGAAG ATGCTGCTAT TGTTGACAAA ATACTCTCTA TGCGCCTAGC TAAAAAAGAG 
CTGCCCACTG GAGAGTATGT TGAAGTTGAA GAGTACTTTG TCAAATATAA AAATTATTCC 
TACCTACACT GTGAATGGGC CACAATTGAG CAACTGGAAA GGGACAAGCG TATTCATCAG 
AAACTGAAAA GATTTAAGAC AAAGATGACC CAGATGCAGC ATTTCTTGCA AGAAGATGAA 
GAATCTTTCA ATCCAGACTA TGTAGAGGTA GATCGAATCC TAGACGAATC TCACAGCACT 
GACAAGGACA ATGGTGAACC GGTGGTGTAT TACCTAGTAA AATGGTGCTC TTTACCTTAT 
GAAGATAGCA CATGGGAGCT GAAAGAAGAT GTTGATGATG GAAAGATAGA GGAATTCAAA 
CGAATAGAGG CGAGGCAGCC AAATCTCAAA CGCGTGGCCC GCCCAGCTGC TACATCATGG 
AAGAAGCTGG AATTGTCTCG GGAGTATCAG AATGGCAACC AGTTGAGAGA ATACCAGCTG 
GAAGGTGTTA ATTGGCTTCT CTTTAACTGG TACAATAGGC AAAACTGCAT TTTGGCAGAT 
GAAATGGGTT TGGGGAAGAC CATCCAATCT ATCACATTCT TACAAGAAGT ATATAATGTG 
GGAATACGGG GACCTTTTTT AGTAATTGCT CCACTTTCCA CCATCACGAA CTGGGAGAGA 
GAGTTTGGCA GTTGGACTCA AATGAATACT ATTGTGTATC ATGGCAGCCT TGCTAGTCGC 
CAAATGATCC AGCAATATGA GATGTACTGT AAGGACTCCA AGGGTCGTCT TATTCCTGGT 
GCGTACAAGT TTGATGCTCT TATTACAACT TTTGAAATGG TGCTGTCTGA TTGCCCAGAG 
CTGCGTGAAA TTGAGTGGCG CTGTGTCATA ATTGATGAGG CTCATCGGCT CAAAAACAGA 
AACTGCAAAC TACTGGACAG CCTAAAGCAC ATGGACTTGG AGCACAAAGT CCTTTTAACA 
GGCACTCCAT TACAGAACAC CGTGGAAGAG TTGTTCAGTC TTTTGCATTT CCTGGAGCCA 
ACACAATTTT CCTCTGAAGC AGAGTTTCTT AAGGACTTTG GAGACCTGAA GACAGAGGAA 
CAGGTGCAGA AATTACAGGC CATCTTAAAA CCTATGATGT TGAGGCGATT AAAGGAAGAT 
GTTGAGAAAA ACCTGGCCCC GAAACAAGAA ACTATAATTG AGGTTGAGCT TACTAACATC 
CAGAAGAAGT ATTACCGAGC AATACTGGAG AAGAATTTTT CTTTTCTGAC CAAAGGAGCT 
AGTCAATCCA ACACACCCAA CTTGCTCAAT ACCATGATGG AACTACGCAA ATGCTGCAAC 
CACCCATACC TCATCACTGG AGCAGAAGAA AAGATCATAT CTGAGTTCCG AGAAGCAACT 
CCAGTAGTTC CCCCTGACTT TCACGTCCAA GCAATGGTAC GCTCCTCTGG CAAACTGGTG 
CTAATAGATA AGCTGCTCCC TAAACTAAGG GCTGGAGGCC ACAAAGTTCT CATCTTTTCT 
CAGATGGTAC GGTGCCTGGA CATCCTGGAA GACTATCTCA TTCAGAGAAG GTACCTTTAT 
GAGCGTATTG ATGGACGTGT CCGTGGCAAC ATGCGTCAGG CAGCCATTGA TAGATTCAGC 
CGCCCAGATT CTGACCGATT TGTGTTTCTA CTCTGTACAC GAGCTGGTGG GCTTGGAATT 
AACTTGACAG CTGCTGATAC TTGTATTATA TTTGACTCGG ACTGGAACCC TCAAAATGAC 
CTTCAGGCTC AAGCTCGATG TCATCGTATT GGGCAAAGCA AAGCCGTTAA AATTTACAGA 
CTCATCACTC GCAACTCCTA TGAGAGAGAG ATGTTTGATA AGGCCAGTCT CAAACTAGGA 
CTAGACAAAG CTGTATTGCA GTCTATGAGT GGTCGGGATA ATCACCTTAG TGGGCCTATT 
CAGCAATTCA CAAAGAAAGA AATTGAAGAC TTGCTAAGGA AAGGAGCTTA TGCTGCAATC 
ATGGATGAAG ATGACGAAGG CTCTAAATTC TGTGAAGAAG ATATTGACCA AATTTTACTG 
AGGCGTACCA CGACTATTAC CATAGAGTCA GAGGGAAAGG GCTCAACCTT CTCCAAGGCA 
AGTTTTGTTG CTTCTGAGAA CAGGACTGAT ATATCCCTAG ATGATCCTAA TTTCTGGCAA 
AAGTGGGCCA AGAAAGCAGA CTTAGACTTG GATCTTCTTA GCAGCAAGAA TACACTGGTT 
ATTGATACCC CACGTATCAG GAAGCAAACA CGGCACTTTA CTAACAAAGA TGATGACATG 
GTTGAGTTCT CAGATTTGGA GAGTGATGAT GATGATCGCC CCAAAGCTCG GCGTCAAGAC 
CGCAAGCATG GATATGGACG TACAGATTGC TTCCGTGTGG AGAAGCATTT GCTTGTTTAT 
GGATGGGGTC GCTGGCGAGA TATCCTTACT CATGGTCGTT TCAAGCGTGG TATGAATGAG 
CGTGACGTGG AGAAAATATG TAGAGCCATA CTGGTTTATT GTCTTTTGCA CTACCGGGGA 
GATGAAAACA TAAAGAGCTT TATCTGGGAT CTAATTACAC CAGCTGAGAA TGGCAAAACT 
AAGCAGCTAC AGAACCACTC TGGATTGTCT ATTCCTGTCC CCCGTGGTCG TAAGGGGAAA 
AAAGTGAAAT CCCAAAGCAG CTTTGACATA CATAAAGCCG ATTGGATACA TAAGTACAAC 
CCAGATACCT TGTTCCAAGA TGAAGGGTAC AAGAAACATC TCAAGCATCA ATGTAACAAG 
GTGCTGCTTC GCGTTCGTAT GTTATATTTT CTTCGTCAGG AAGTTATTGG AAACCAGGCA 
ATGAAAGTTT TAAGTGGTGT AGAGGCCAGG CAAATTGACA TCTGGTTCCC ACAGGTGGAC 
CAGGTTGAAG TTCCCTCACG CTGGTGGGAC AGAGAAGCTG ATAAATCTCT TCTTATCGGG 
GTCTTTAAAC ATGGTTATGA GAAGTATAAC ACCATGCGAG CTGACCCTGC ACTCTGTTTT 
TTGGAGAAGG TGGGGGGACC AGATGAACAG GCTATTGCAG CTGAGCACCA TGCTCAAGAC 
TTTTCTGAAC TCCCTGATGG TGGGGACTTT GATCGAGACA TAGAAGATCC AGAATACAAG 
CCACTTCATG CACAGAAAGA TCCTGAGGAT GAGATCGACT CTTTAATGAT GGATGAAGAA 
ATCTCTGTTG TTGACGGTGA AGAAGCAACA GCAATGCCCA GTGGTATGTT ATGGCCACCT 
GGCTCTGCTC TCACAGCTCG TCTACGGCGT CTAATTACTG CCTACCAACG CAGCTACAAA 
AGAGAACAAC TAAAATTGGA GGCTGAAGAG CGTGGGGACA AACGTAGAAA ACGATGTGAG 
GCAGCCTTTA AACTGAAAGA AATTGCCCGT AGGGAGAAAC AGCAAAGGTG GACCCGAAGG 
GAGGCATGTG ACTTTTTTCG TGTGCTGTCC AGCTTTGGTG TCGAGTATGA CCCCGATACT 
CAACTGTATG ACTGGCAGAG GTTCCGTGGT CTGGCAAGAC TGGACAAGAA GACAGATGAG 
TCACTGCTCA AATACTTCCA TGGATTTGTG GCAATGTGCC GCCAGGTTTG CCGCTTACCC 
CCTGCTGCCG GAGACGAGCC ACCAGATCCA TCTCTCTTCA TTGAGCCAAT TTCAGAAGAG 
CGAGCATCTC GCGCCCTGTT TCGCTTGGAT CTTCTTCGTA GGGTGCGTGA GCAAGTACTT 
TGTCATCCTT TGCTTATTCC TCGTTTGTCA CTGTGCCGGC CAGACCCAGA ACTCCCAGAA 
TGGTGGGAGA GTGGACGTCA TGACAATGAG CTTCTTCAGG GAGCAGCACG TTATGGTCTG 
AGCCGTACAG ACCTCACTAT ACTGCAGGAC CATGCATTTT CTTTTTTGCG GTGTCAGATC 
AGTTATTTCC AGTCAAGGGG AATATCTGGC CATTCTCGCC CTTCTACTCC AACTACTGCC 
CCTTCAGTGC CTACTGAACG TCAGCCTTCT CATCTAGCCT CCCTTTCCTC ATCTGTCAGC 
TGTTCTCCTG CTCCACGCAG ATCCTCTTCC TGCTCATCAT CATCTCACTC ATCAAACTCT 
GAGGATTCTT CAGATGAAAG CAATGGTGTA AAAGTAAAAC CTAATGCAGG TCTTTCGCAC 
GCTCGACTGT ATGATGAGGA GAGCCGGCTT TCTCTTACTG CCTCCCCTGC AGATCTCACT 
ACAGAGGACA GTATTCAGAC AGCGCTGGAG ACCCCGCACA GTACAGACTG GCCAAAGGAC 
CGTGTCCTTA TCTGCAGAAT TGAGCAAGTA TGCAGTGCCG TTCTTACTGG CAAATGGTCC 
TCACCACGAC GACTGTCAGA TCCACCAAGT GACAGCCCTG ATAGCTTGCC TCCAACCCCA 
GAGCAGCAGA GTCCAGCCCA CTTCACACAG ATAAGACCTG CACCGGACCC CAAAGAGTTT 
ACCATTCAAA TAAAAAGTGA AGAAGGCCTG AAGATTAAAT TTCAGAAGCA CAAATTTATG 
GGTAATGGAG CTCTTGATTC TGCACCTCAT ACCCAGAAGA AAAAGAGCAA GAAAAAGATG 
GATCTTGACA TGGACACACG AATTCCCGTT GTAAACCAGA AGGATGGGAC CTTGTTGTTA 
GGGGAAGAAG CCCCTTTACG CTGCCAGCTG CCCGAGTGGT TACACAGAAA TCCTGACTTC 
ATGGTTGACC CACGCTTTAT TGCGTTTATG GAAGACCGCA GGAAAAGAAA AAAACATCGA 
AGCAAAAAGT CCAAATCCTC CTCCCAGATT GGAAACGTAC AGAATCCCCA CTCCCACAAT 
GGAAAGAAGT CTTCCCACAC AGAAAACTCT TTCGAGACGC AACACCTTCC TACTGCAGCC 
AAAAAAAGGA AGAAACGCCC TCCTTCAAAA ATAATAATGC CATCTGCACT ACATGGAGCT 
GGTGATGTTA CACATTCCTT CTCAGACCTT CAACCTGACC ATTCTGGTCA CCCCATGCGG 
TCAGTGTATG GACCATCAGA TGGAGGGCTG TCCCATCAAG CAGGACTCAG CGGGTTGGGA 
TTTACCCGCT TTCATTCATC GCAGACATCT TCAGTGGGCA GAATCCATGG CACTACCTTT 
GATGAGTTAC AAAGTTTTGA GCCCCCACAA CAACATTTTT CTCTGCTTGA TGATCCTATG 
ATGCCAGCAA ATTCCTCCTC CAGCGGTGAT GAACTGGACA GACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chd8 ( XM_012957172.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012812626.1 Xenopus tropicalis chromodomain helicase DNA binding protein 8 (chd8), transcript variant X1, mRNA.

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CloneID	OXa32500
Clone ID Related Accession (Same CDS sequence)	XM_012957172.3 , XM_012957178.3 , XM_031896082.1 , XM_012957178.2 , XM_012957172.2 , XM_012957182.2 , XM_012957182.3
Accession Version	XM_012957172.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7008bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	chromodomain-helicase-DNA-binding protein 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957172.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACC CTATCATGGA CCTCTTTGAT GATCCAAACC TATTTGGCCT TGACTCTCTC 
ACTGGAGATT CCTTTGGACG GGATGGGCCA GACACTATAG ATGATGCTCT AGGCCTGGGT 
AATGTCTTGG GACCTTTGGA GCCGATAACA GACAGAGTTG GCCTGCCTGG TGCTGATGTT 
GGCAATAATG AAGTTAAACA ACAAACAGAG TCACAGGTTC TCCCGGACAA TCCAGCTCTT 
ATGTCAGCTA CTGAGATCAT GCAGCCCATT CAACTTCCCA CAAACCAGGA AACACTGAAC 
CAAGGAAATC CCTTTATGGG TGCCTCAAAT GCTGGAGCCC CAAAAATTAT GATTCTGAAA 
GCTCCTGCTG GAATGACAGT TGGTGCATGT CCTGTAACTC AGATACAAAC TCTGACACCT 
CACCAAGCTG CTAATGGTGG CAAGGTGGCA TTTGCTAAGG TGCTTACAGG AACCCCATTG 
CGTCCTGGGG TCTCAATTGT ATCTGGAAAC ACTGTCCTTG CCAAAATGCC AAGCACTGGA 
GTTGCAGGGC AGGTAGGAGC AGTGAGGCCT GTCCGTCAGC TACTTCTTCA ACCAGTGCGT 
GCCTCCGCAG CCCCAGGAAG CTCCGAGAGT AATACAGGGA TCAAGCCTGC AATCACACTA 
ACGTCAGCAC CTCAACAGGG GGACCCTAAA AGAATTACTC TCGTCCTACA GCAGCCTTCC 
CAAGTTGGTG CAACTGCACA ACAAGGGCAG CGTCATGTTG TGCTGGGTGG GCTTCCAGGA 
AAGATTGTCC TTCAGGGAAA CCAACTGGCA GCTCTCACAC AAGCTAAAAC CCCTCAAGGT 
CAGCCAGCTA AAGTAGTGAC TATCCAGCTG CAAGTCCAGC AGCAGCCTGG GGCTGCAGGT 
CAGACTCCTC AAAAGTTTCA GATTGTACAA CAAGCCCCAG GAGGTGTGGC AATTGCTCCC 
AGTGGGCAAC ATACACACAT GCTTAATCAA CAAGGAGTTC AGAGGCTTTC TGTTCCACTC 
AAAGTTGTTC TGCAACCACA GGCTGGTTCC TCTCAGGGTA CTGCGCAGGG CCTGTCAGTT 
GTGAAAGTTC TAAATGCAAG TGAAGTTGCC AATTTGACTG CAAGTCAGAC TGTGGTTAAA 
ACATCCACTG GAGGTGGTGA ATCTCGTAAG CTGGACAGCC AGAAGAAGCA AGAGAAAGCT 
AACCGTATTG TAGCTGAAGC TATTGCCAGG GCCCGAGCCA GAGGTGAACA GAATATTCCT 
CGGGTCCTGA ATGAGGATGA GCTTCCCAGT GTGAATCCTG AAGATGATGA TGGAAGCAGG 
CGGAAAAGGA AGAAGAAGAA AGGAGAGACT AGTGATAGGT CCAAGGATGA GAAACCTAAG 
AAGGTCAAAG GTAGTGGAAG CTTAAGGTCT AGGAGCAAAG GCAAACCAAG TACTATCACT 
CCTATTGTGG GTAAGAAAAG GAAGAGAAAC CCATCTTCCG ATAATTCTGA TGCTGAGATC 
ATGGCATCAC AGGCTTCTCC TGCAGAGGAT GAAGACAGTG TCCAGAAGAG ACGTTCAAAT 
CGCCAGGTGA AGCGCAAGAA GTATACAGAG GATTTGGATA TTAAAATAAC AGATGATGAA 
GATGATGAGG AGGTAGATGT GACGGGACCT GTGAAAACAG AACCTGTGCT GCTTCCTGAG 
CCCATGCCTT TACCTGACAG TGAGGCAGTC CCTTCAATGC AGTTCTTCGT GGAGAACCCA 
AGTGAAGAAG ATGCTGCTAT TGTTGACAAA ATACTCTCTA TGCGCCTAGC TAAAAAAGAG 
CTGCCCACTG GAGAGTATGT TGAAGTTGAA GAGTACTTTG TCAAATATAA AAATTATTCC 
TACCTACACT GTGAATGGGC CACAATTGAG CAACTGGAAA GGGACAAGCG TATTCATCAG 
AAACTGAAAA GATTTAAGAC AAAGATGACC CAGATGCAGC ATTTCTTGCA AGAAGATGAA 
GAATCTTTCA ATCCAGACTA TGTAGAGGTA GATCGAATCC TAGACGAATC TCACAGCACT 
GACAAGGACA ATGGTGAACC GGTGGTGTAT TACCTAGTAA AATGGTGCTC TTTACCTTAT 
GAAGATAGCA CATGGGAGCT GAAAGAAGAT GTTGATGATG GAAAGATAGA GGAATTCAAA 
CGAATAGAGG CGAGGCAGCC AAATCTCAAA CGCGTGGCCC GCCCAGCTGC TACATCATGG 
AAGAAGCTGG AATTGTCTCG GGAGTATCAG AATGGCAACC AGTTGAGAGA ATACCAGCTG 
GAAGGTGTTA ATTGGCTTCT CTTTAACTGG TACAATAGGC AAAACTGCAT TTTGGCAGAT 
GAAATGGGTT TGGGGAAGAC CATCCAATCT ATCACATTCT TACAAGAAGT ATATAATGTG 
GGAATACGGG GACCTTTTTT AGTAATTGCT CCACTTTCCA CCATCACGAA CTGGGAGAGA 
GAGTTTGGCA GTTGGACTCA AATGAATACT ATTGTGTATC ATGGCAGCCT TGCTAGTCGC 
CAAATGATCC AGCAATATGA GATGTACTGT AAGGACTCCA AGGGTCGTCT TATTCCTGGT 
GCGTACAAGT TTGATGCTCT TATTACAACT TTTGAAATGG TGCTGTCTGA TTGCCCAGAG 
CTGCGTGAAA TTGAGTGGCG CTGTGTCATA ATTGATGAGG CTCATCGGCT CAAAAACAGA 
AACTGCAAAC TACTGGACAG CCTAAAGCAC ATGGACTTGG AGCACAAAGT CCTTTTAACA 
GGCACTCCAT TACAGAACAC CGTGGAAGAG TTGTTCAGTC TTTTGCATTT CCTGGAGCCA 
ACACAATTTT CCTCTGAAGC AGAGTTTCTT AAGGACTTTG GAGACCTGAA GACAGAGGAA 
CAGGTGCAGA AATTACAGGC CATCTTAAAA CCTATGATGT TGAGGCGATT AAAGGAAGAT 
GTTGAGAAAA ACCTGGCCCC GAAACAAGAA ACTATAATTG AGGTTGAGCT TACTAACATC 
CAGAAGAAGT ATTACCGAGC AATACTGGAG AAGAATTTTT CTTTTCTGAC CAAAGGAGCT 
AGTCAATCCA ACACACCCAA CTTGCTCAAT ACCATGATGG AACTACGCAA ATGCTGCAAC 
CACCCATACC TCATCACTGG AGCAGAAGAA AAGATCATAT CTGAGTTCCG AGAAGCAACT 
CCAGTAGTTC CCCCTGACTT TCACGTCCAA GCAATGGTAC GCTCCTCTGG CAAACTGGTG 
CTAATAGATA AGCTGCTCCC TAAACTAAGG GCTGGAGGCC ACAAAGTTCT CATCTTTTCT 
CAGATGGTAC GGTGCCTGGA CATCCTGGAA GACTATCTCA TTCAGAGAAG GTACCTTTAT 
GAGCGTATTG ATGGACGTGT CCGTGGCAAC ATGCGTCAGG CAGCCATTGA TAGATTCAGC 
CGCCCAGATT CTGACCGATT TGTGTTTCTA CTCTGTACAC GAGCTGGTGG GCTTGGAATT 
AACTTGACAG CTGCTGATAC TTGTATTATA TTTGACTCGG ACTGGAACCC TCAAAATGAC 
CTTCAGGCTC AAGCTCGATG TCATCGTATT GGGCAAAGCA AAGCCGTTAA AATTTACAGA 
CTCATCACTC GCAACTCCTA TGAGAGAGAG ATGTTTGATA AGGCCAGTCT CAAACTAGGA 
CTAGACAAAG CTGTATTGCA GTCTATGAGT GGTCGGGATA ATCACCTTAG TGGGCCTATT 
CAGCAATTCA CAAAGAAAGA AATTGAAGAC TTGCTAAGGA AAGGAGCTTA TGCTGCAATC 
ATGGATGAAG ATGACGAAGG CTCTAAATTC TGTGAAGAAG ATATTGACCA AATTTTACTG 
AGGCGTACCA CGACTATTAC CATAGAGTCA GAGGGAAAGG GCTCAACCTT CTCCAAGGCA 
AGTTTTGTTG CTTCTGAGAA CAGGACTGAT ATATCCCTAG ATGATCCTAA TTTCTGGCAA 
AAGTGGGCCA AGAAAGCAGA CTTAGACTTG GATCTTCTTA GCAGCAAGAA TACACTGGTT 
ATTGATACCC CACGTATCAG GAAGCAAACA CGGCACTTTA CTAACAAAGA TGATGACATG 
GTTGAGTTCT CAGATTTGGA GAGTGATGAT GATGATCGCC CCAAAGCTCG GCGTCAAGAC 
CGCAAGCATG GATATGGACG TACAGATTGC TTCCGTGTGG AGAAGCATTT GCTTGTTTAT 
GGATGGGGTC GCTGGCGAGA TATCCTTACT CATGGTCGTT TCAAGCGTGG TATGAATGAG 
CGTGACGTGG AGAAAATATG TAGAGCCATA CTGGTTTATT GTCTTTTGCA CTACCGGGGA 
GATGAAAACA TAAAGAGCTT TATCTGGGAT CTAATTACAC CAGCTGAGAA TGGCAAAACT 
AAGCAGCTAC AGAACCACTC TGGATTGTCT ATTCCTGTCC CCCGTGGTCG TAAGGGGAAA 
AAAGTGAAAT CCCAAAGCAG CTTTGACATA CATAAAGCCG ATTGGATACA TAAGTACAAC 
CCAGATACCT TGTTCCAAGA TGAAGGGTAC AAGAAACATC TCAAGCATCA ATGTAACAAG 
GTGCTGCTTC GCGTTCGTAT GTTATATTTT CTTCGTCAGG AAGTTATTGG AAACCAGGCA 
ATGAAAGTTT TAAGTGGTGT AGAGGCCAGG CAAATTGACA TCTGGTTCCC ACAGGTGGAC 
CAGGTTGAAG TTCCCTCACG CTGGTGGGAC AGAGAAGCTG ATAAATCTCT TCTTATCGGG 
GTCTTTAAAC ATGGTTATGA GAAGTATAAC ACCATGCGAG CTGACCCTGC ACTCTGTTTT 
TTGGAGAAGG TGGGGGGACC AGATGAACAG GCTATTGCAG CTGAGCACCA TGCTCAAGAC 
TTTTCTGAAC TCCCTGATGG TGGGGACTTT GATCGAGACA TAGAAGATCC AGAATACAAG 
CCACTTCATG CACAGAAAGA TCCTGAGGAT GAGATCGACT CTTTAATGAT GGATGAAGAA 
ATCTCTGTTG TTGACGGTGA AGAAGCAACA GCAATGCCCA GTGGTATGTT ATGGCCACCT 
GGCTCTGCTC TCACAGCTCG TCTACGGCGT CTAATTACTG CCTACCAACG CAGCTACAAA 
AGAGAACAAC TAAAATTGGA GGCTGAAGAG CGTGGGGACA AACGTAGAAA ACGATGTGAG 
GCAGCCTTTA AACTGAAAGA AATTGCCCGT AGGGAGAAAC AGCAAAGGTG GACCCGAAGG 
GAGGCATGTG ACTTTTTTCG TGTGCTGTCC AGCTTTGGTG TCGAGTATGA CCCCGATACT 
CAACTGTATG ACTGGCAGAG GTTCCGTGGT CTGGCAAGAC TGGACAAGAA GACAGATGAG 
TCACTGCTCA AATACTTCCA TGGATTTGTG GCAATGTGCC GCCAGGTTTG CCGCTTACCC 
CCTGCTGCCG GAGACGAGCC ACCAGATCCA TCTCTCTTCA TTGAGCCAAT TTCAGAAGAG 
CGAGCATCTC GCGCCCTGTT TCGCTTGGAT CTTCTTCGTA GGGTGCGTGA GCAAGTACTT 
TGTCATCCTT TGCTTATTCC TCGTTTGTCA CTGTGCCGGC CAGACCCAGA ACTCCCAGAA 
TGGTGGGAGA GTGGACGTCA TGACAATGAG CTTCTTCAGG GAGCAGCACG TTATGGTCTG 
AGCCGTACAG ACCTCACTAT ACTGCAGGAC CATGCATTTT CTTTTTTGCG GTGTCAGATC 
AGTTATTTCC AGTCAAGGGG AATATCTGGC CATTCTCGCC CTTCTACTCC AACTACTGCC 
CCTTCAGTGC CTACTGAACG TCAGCCTTCT CATCTAGCCT CCCTTTCCTC ATCTGTCAGC 
TGTTCTCCTG CTCCACGCAG ATCCTCTTCC TGCTCATCAT CATCTCACTC ATCAAACTCT 
GAGGATTCTT CAGATGAAAG CAATGGTGTA AAAGTAAAAC CTAATGCAGG TCTTTCGCAC 
GCTCGACTGT ATGATGAGGA GAGCCGGCTT TCTCTTACTG CCTCCCCTGC AGATCTCACT 
ACAGAGGACA GTATTCAGAC AGCGCTGGAG ACCCCGCACA GTACAGACTG GCCAAAGGAC 
CGTGTCCTTA TCTGCAGAAT TGAGCAAGTA TGCAGTGCCG TTCTTACTGG CAAATGGTCC 
TCACCACGAC GACTGTCAGA TCCACCAAGT GACAGCCCTG ATAGCTTGCC TCCAACCCCA 
GAGCAGCAGA GTCCAGCCCA CTTCACACAG ATAAGACCTG CACCGGACCC CAAAGAGTTT 
ACCATTCAAA TAAAAAGTGA AGAAGGCCTG AAGATTAAAT TTCAGAAGCA CAAATTTATG 
GGTAATGGAG CTCTTGATTC TGCACCTCAT ACCCAGAAGA AAAAGAGCAA GAAAAAGATG 
GATCTTGACA TGGACACACG AATTCCCGTT GTAAACCAGA AGGATGGGAC CTTGTTGTTA 
GGGGAAGAAG CCCCTTTACG CTGCCAGCTG CCCGAGTGGT TACACAGAAA TCCTGACTTC 
ATGGTTGACC CACGCTTTAT TGCGTTTATG GAAGACCGCA GGAAAAGAAA AAAACATCGA 
AGCAAAAAGT CCAAATCCTC CTCCCAGATT GGAAACGTAC AGAATCCCCA CTCCCACAAT 
GGAAAGAAGT CTTCCCACAC AGAAAACTCT TTCGAGACGC AACACCTTCC TACTGCAGCC 
AAAAAAAGGA AGAAACGCCC TCCTTCAAAA ATAATAATGC CATCTGCACT ACATGGAGCT 
GGTGATGTTA CACATTCCTT CTCAGACCTT CAACCTGACC ATTCTGGTCA CCCCATGCGG 
TCAGTGTATG GACCATCAGA TGGAGGGCTG TCCCATCAAG CAGGACTCAG CGGGTTGGGA 
TTTACCCGCT TTCATTCATC GCAGACATCT TCAGTGGGCA GAATCCATGG CACTACCTTT 
GATGAGTTAC AAAGTTTTGA GCCCCCACAA CAACATTTTT CTCTGCTTGA TGATCCTATG 
ATGCCAGCAA ATTCCTCCTC CAGCGGTGAT GAACTGGACA GACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012812626.1
CDS	301..7308
Translation	MADPIMDLFDDPNLFGLDSLTGDSFGRDGPDTIDDALGLGNVLGPLEPITDRVGLPGADVGNNEVKQQTESQVLPDNPALMSATEIMQPIQLPTNQETLNQGNPFMGASNAGAPKIMILKAPAGMTVGACPVTQIQTLTPHQAANGGKVAFAKVLTGTPLRPGVSIVSGNTVLAKMPSTGVAGQVGAVRPVRQLLLQPVRASAAPGSSESNTGIKPAITLTSAPQQGDPKRITLVLQQPSQVGATAQQGQRHVVLGGLPGKIVLQGNQLAALTQAKTPQGQPAKVVTIQLQVQQQPGAAGQTPQKFQIVQQAPGGVAIAPSGQHTHMLNQQGVQRLSVPLKVVLQPQAGSSQGTAQGLSVVKVLNASEVANLTASQTVVKTSTGGGESRKLDSQKKQEKANRIVAEAIARARARGEQNIPRVLNEDELPSVNPEDDDGSRRKRKKKKGETSDRSKDEKPKKVKGSGSLRSRSKGKPSTITPIVGKKRKRNPSSDNSDAEIMASQASPAEDEDSVQKRRSNRQVKRKKYTEDLDIKITDDEDDEEVDVTGPVKTEPVLLPEPMPLPDSEAVPSMQFFVENPSEEDAAIVDKILSMRLAKKELPTGEYVEVEEYFVKYKNYSYLHCEWATIEQLERDKRIHQKLKRFKTKMTQMQHFLQEDEESFNPDYVEVDRILDESHSTDKDNGEPVVYYLVKWCSLPYEDSTWELKEDVDDGKIEEFKRIEARQPNLKRVARPAATSWKKLELSREYQNGNQLREYQLEGVNWLLFNWYNRQNCILADEMGLGKTIQSITFLQEVYNVGIRGPFLVIAPLSTITNWEREFGSWTQMNTIVYHGSLASRQMIQQYEMYCKDSKGRLIPGAYKFDALITTFEMVLSDCPELREIEWRCVIIDEAHRLKNRNCKLLDSLKHMDLEHKVLLTGTPLQNTVEELFSLLHFLEPTQFSSEAEFLKDFGDLKTEEQVQKLQAILKPMMLRRLKEDVEKNLAPKQETIIEVELTNIQKKYYRAILEKNFSFLTKGASQSNTPNLLNTMMELRKCCNHPYLITGAEEKIISEFREATPVVPPDFHVQAMVRSSGKLVLIDKLLPKLRAGGHKVLIFSQMVRCLDILEDYLIQRRYLYERIDGRVRGNMRQAAIDRFSRPDSDRFVFLLCTRAGGLGINLTAADTCIIFDSDWNPQNDLQAQARCHRIGQSKAVKIYRLITRNSYEREMFDKASLKLGLDKAVLQSMSGRDNHLSGPIQQFTKKEIEDLLRKGAYAAIMDEDDEGSKFCEEDIDQILLRRTTTITIESEGKGSTFSKASFVASENRTDISLDDPNFWQKWAKKADLDLDLLSSKNTLVIDTPRIRKQTRHFTNKDDDMVEFSDLESDDDDRPKARRQDRKHGYGRTDCFRVEKHLLVYGWGRWRDILTHGRFKRGMNERDVEKICRAILVYCLLHYRGDENIKSFIWDLITPAENGKTKQLQNHSGLSIPVPRGRKGKKVKSQSSFDIHKADWIHKYNPDTLFQDEGYKKHLKHQCNKVLLRVRMLYFLRQEVIGNQAMKVLSGVEARQIDIWFPQVDQVEVPSRWWDREADKSLLIGVFKHGYEKYNTMRADPALCFLEKVGGPDEQAIAAEHHAQDFSELPDGGDFDRDIEDPEYKPLHAQKDPEDEIDSLMMDEEISVVDGEEATAMPSGMLWPPGSALTARLRRLITAYQRSYKREQLKLEAEERGDKRRKRCEAAFKLKEIARREKQQRWTRREACDFFRVLSSFGVEYDPDTQLYDWQRFRGLARLDKKTDESLLKYFHGFVAMCRQVCRLPPAAGDEPPDPSLFIEPISEERASRALFRLDLLRRVREQVLCHPLLIPRLSLCRPDPELPEWWESGRHDNELLQGAARYGLSRTDLTILQDHAFSFLRCQISYFQSRGISGHSRPSTPTTAPSVPTERQPSHLASLSSSVSCSPAPRRSSSCSSSSHSSNSEDSSDESNGVKVKPNAGLSHARLYDEESRLSLTASPADLTTEDSIQTALETPHSTDWPKDRVLICRIEQVCSAVLTGKWSSPRRLSDPPSDSPDSLPPTPEQQSPAHFTQIRPAPDPKEFTIQIKSEEGLKIKFQKHKFMGNGALDSAPHTQKKKSKKKMDLDMDTRIPVVNQKDGTLLLGEEAPLRCQLPEWLHRNPDFMVDPRFIAFMEDRRKRKKHRSKKSKSSSQIGNVQNPHSHNGKKSSHTENSFETQHLPTAAKKRKKRPPSKIIMPSALHGAGDVTHSFSDLQPDHSGHPMRSVYGPSDGGLSHQAGLSGLGFTRFHSSQTSSVGRIHGTTFDELQSFEPPQQHFSLLDDPMMPANSSSSGDELDRP

Target ORF information:

RefSeq Version	XM_012957172.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis chromodomain helicase DNA binding protein 8 (chd8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957172.2

ORF Insert Sequence:

ATGGCTGACC CTATCATGGA CCTCTTTGAT GATCCAAACC TATTTGGCCT TGACTCTCTC 
ACTGGAGATT CCTTTGGACG GGATGGGCCA GACACTATAG ATGATGCTCT AGGCCTGGGT 
AATGTCTTGG GACCTTTGGA GCCGATAACA GACAGAGTTG GCCTGCCTGG TGCTGATGTT 
GGCAATAATG AAGTTAAACA ACAAACAGAG TCACAGGTTC TCCCGGACAA TCCAGCTCTT 
ATGTCAGCTA CTGAGATCAT GCAGCCCATT CAACTTCCCA CAAACCAGGA AACACTGAAC 
CAAGGAAATC CCTTTATGGG TGCCTCAAAT GCTGGAGCCC CAAAAATTAT GATTCTGAAA 
GCTCCTGCTG GAATGACAGT TGGTGCATGT CCTGTAACTC AGATACAAAC TCTGACACCT 
CACCAAGCTG CTAATGGTGG CAAGGTGGCA TTTGCTAAGG TGCTTACAGG AACCCCATTG 
CGTCCTGGGG TCTCAATTGT ATCTGGAAAC ACTGTCCTTG CCAAAATGCC AAGCACTGGA 
GTTGCAGGGC AGGTAGGAGC AGTGAGGCCT GTCCGTCAGC TACTTCTTCA ACCAGTGCGT 
GCCTCCGCAG CCCCAGGAAG CTCCGAGAGT AATACAGGGA TCAAGCCTGC AATCACACTA 
ACGTCAGCAC CTCAACAGGG GGACCCTAAA AGAATTACTC TCGTCCTACA GCAGCCTTCC 
CAAGTTGGTG CAACTGCACA ACAAGGGCAG CGTCATGTTG TGCTGGGTGG GCTTCCAGGA 
AAGATTGTCC TTCAGGGAAA CCAACTGGCA GCTCTCACAC AAGCTAAAAC CCCTCAAGGT 
CAGCCAGCTA AAGTAGTGAC TATCCAGCTG CAAGTCCAGC AGCAGCCTGG GGCTGCAGGT 
CAGACTCCTC AAAAGTTTCA GATTGTACAA CAAGCCCCAG GAGGTGTGGC AATTGCTCCC 
AGTGGGCAAC ATACACACAT GCTTAATCAA CAAGGAGTTC AGAGGCTTTC TGTTCCACTC 
AAAGTTGTTC TGCAACCACA GGCTGGTTCC TCTCAGGGTA CTGCGCAGGG CCTGTCAGTT 
GTGAAAGTTC TAAATGCAAG TGAAGTTGCC AATTTGACTG CAAGTCAGAC TGTGGTTAAA 
ACATCCACTG GAGGTGGTGA ATCTCGTAAG CTGGACAGCC AGAAGAAGCA AGAGAAAGCT 
AACCGTATTG TAGCTGAAGC TATTGCCAGG GCCCGAGCCA GAGGTGAACA GAATATTCCT 
CGGGTCCTGA ATGAGGATGA GCTTCCCAGT GTGAATCCTG AAGATGATGA TGGAAGCAGG 
CGGAAAAGGA AGAAGAAGAA AGGAGAGACT AGTGATAGGT CCAAGGATGA GAAACCTAAG 
AAGGTCAAAG GTAGTGGAAG CTTAAGGTCT AGGAGCAAAG GCAAACCAAG TACTATCACT 
CCTATTGTGG GTAAGAAAAG GAAGAGAAAC CCATCTTCCG ATAATTCTGA TGCTGAGATC 
ATGGCATCAC AGGCTTCTCC TGCAGAGGAT GAAGACAGTG TCCAGAAGAG ACGTTCAAAT 
CGCCAGGTGA AGCGCAAGAA GTATACAGAG GATTTGGATA TTAAAATAAC AGATGATGAA 
GATGATGAGG AGGTAGATGT GACGGGACCT GTGAAAACAG AACCTGTGCT GCTTCCTGAG 
CCCATGCCTT TACCTGACAG TGAGGCAGTC CCTTCAATGC AGTTCTTCGT GGAGAACCCA 
AGTGAAGAAG ATGCTGCTAT TGTTGACAAA ATACTCTCTA TGCGCCTAGC TAAAAAAGAG 
CTGCCCACTG GAGAGTATGT TGAAGTTGAA GAGTACTTTG TCAAATATAA AAATTATTCC 
TACCTACACT GTGAATGGGC CACAATTGAG CAACTGGAAA GGGACAAGCG TATTCATCAG 
AAACTGAAAA GATTTAAGAC AAAGATGACC CAGATGCAGC ATTTCTTGCA AGAAGATGAA 
GAATCTTTCA ATCCAGACTA TGTAGAGGTA GATCGAATCC TAGACGAATC TCACAGCACT 
GACAAGGACA ATGGTGAACC GGTGGTGTAT TACCTAGTAA AATGGTGCTC TTTACCTTAT 
GAAGATAGCA CATGGGAGCT GAAAGAAGAT GTTGATGATG GAAAGATAGA GGAATTCAAA 
CGAATAGAGG CGAGGCAGCC AAATCTCAAA CGCGTGGCCC GCCCAGCTGC TACATCATGG 
AAGAAGCTGG AATTGTCTCG GGAGTATCAG AATGGCAACC AGTTGAGAGA ATACCAGCTG 
GAAGGTGTTA ATTGGCTTCT CTTTAACTGG TACAATAGGC AAAACTGCAT TTTGGCAGAT 
GAAATGGGTT TGGGGAAGAC CATCCAATCT ATCACATTCT TACAAGAAGT ATATAATGTG 
GGAATACGGG GACCTTTTTT AGTAATTGCT CCACTTTCCA CCATCACGAA CTGGGAGAGA 
GAGTTTGGCA GTTGGACTCA AATGAATACT ATTGTGTATC ATGGCAGCCT TGCTAGTCGC 
CAAATGATCC AGCAATATGA GATGTACTGT AAGGACTCCA AGGGTCGTCT TATTCCTGGT 
GCGTACAAGT TTGATGCTCT TATTACAACT TTTGAAATGG TGCTGTCTGA TTGCCCAGAG 
CTGCGTGAAA TTGAGTGGCG CTGTGTCATA ATTGATGAGG CTCATCGGCT CAAAAACAGA 
AACTGCAAAC TACTGGACAG CCTAAAGCAC ATGGACTTGG AGCACAAAGT CCTTTTAACA 
GGCACTCCAT TACAGAACAC CGTGGAAGAG TTGTTCAGTC TTTTGCATTT CCTGGAGCCA 
ACACAATTTT CCTCTGAAGC AGAGTTTCTT AAGGACTTTG GAGACCTGAA GACAGAGGAA 
CAGGTGCAGA AATTACAGGC CATCTTAAAA CCTATGATGT TGAGGCGATT AAAGGAAGAT 
GTTGAGAAAA ACCTGGCCCC GAAACAAGAA ACTATAATTG AGGTTGAGCT TACTAACATC 
CAGAAGAAGT ATTACCGAGC AATACTGGAG AAGAATTTTT CTTTTCTGAC CAAAGGAGCT 
AGTCAATCCA ACACACCCAA CTTGCTCAAT ACCATGATGG AACTACGCAA ATGCTGCAAC 
CACCCATACC TCATCACTGG AGCAGAAGAA AAGATCATAT CTGAGTTCCG AGAAGCAACT 
CCAGTAGTTC CCCCTGACTT TCACGTCCAA GCAATGGTAC GCTCCTCTGG CAAACTGGTG 
CTAATAGATA AGCTGCTCCC TAAACTAAGG GCTGGAGGCC ACAAAGTTCT CATCTTTTCT 
CAGATGGTAC GGTGCCTGGA CATCCTGGAA GACTATCTCA TTCAGAGAAG GTACCTTTAT 
GAGCGTATTG ATGGACGTGT CCGTGGCAAC ATGCGTCAGG CAGCCATTGA TAGATTCAGC 
CGCCCAGATT CTGACCGATT TGTGTTTCTA CTCTGTACAC GAGCTGGTGG GCTTGGAATT 
AACTTGACAG CTGCTGATAC TTGTATTATA TTTGACTCGG ACTGGAACCC TCAAAATGAC 
CTTCAGGCTC AAGCTCGATG TCATCGTATT GGGCAAAGCA AAGCCGTTAA AATTTACAGA 
CTCATCACTC GCAACTCCTA TGAGAGAGAG ATGTTTGATA AGGCCAGTCT CAAACTAGGA 
CTAGACAAAG CTGTATTGCA GTCTATGAGT GGTCGGGATA ATCACCTTAG TGGGCCTATT 
CAGCAATTCA CAAAGAAAGA AATTGAAGAC TTGCTAAGGA AAGGAGCTTA TGCTGCAATC 
ATGGATGAAG ATGACGAAGG CTCTAAATTC TGTGAAGAAG ATATTGACCA AATTTTACTG 
AGGCGTACCA CGACTATTAC CATAGAGTCA GAGGGAAAGG GCTCAACCTT CTCCAAGGCA 
AGTTTTGTTG CTTCTGAGAA CAGGACTGAT ATATCCCTAG ATGATCCTAA TTTCTGGCAA 
AAGTGGGCCA AGAAAGCAGA CTTAGACTTG GATCTTCTTA GCAGCAAGAA TACACTGGTT 
ATTGATACCC CACGTATCAG GAAGCAAACA CGGCACTTTA CTAACAAAGA TGATGACATG 
GTTGAGTTCT CAGATTTGGA GAGTGATGAT GATGATCGCC CCAAAGCTCG GCGTCAAGAC 
CGCAAGCATG GATATGGACG TACAGATTGC TTCCGTGTGG AGAAGCATTT GCTTGTTTAT 
GGATGGGGTC GCTGGCGAGA TATCCTTACT CATGGTCGTT TCAAGCGTGG TATGAATGAG 
CGTGACGTGG AGAAAATATG TAGAGCCATA CTGGTTTATT GTCTTTTGCA CTACCGGGGA 
GATGAAAACA TAAAGAGCTT TATCTGGGAT CTAATTACAC CAGCTGAGAA TGGCAAAACT 
AAGCAGCTAC AGAACCACTC TGGATTGTCT ATTCCTGTCC CCCGTGGTCG TAAGGGGAAA 
AAAGTGAAAT CCCAAAGCAG CTTTGACATA CATAAAGCCG ATTGGATACA TAAGTACAAC 
CCAGATACCT TGTTCCAAGA TGAAGGGTAC AAGAAACATC TCAAGCATCA ATGTAACAAG 
GTGCTGCTTC GCGTTCGTAT GTTATATTTT CTTCGTCAGG AAGTTATTGG AAACCAGGCA 
ATGAAAGTTT TAAGTGGTGT AGAGGCCAGG CAAATTGACA TCTGGTTCCC ACAGGTGGAC 
CAGGTTGAAG TTCCCTCACG CTGGTGGGAC AGAGAAGCTG ATAAATCTCT TCTTATCGGG 
GTCTTTAAAC ATGGTTATGA GAAGTATAAC ACCATGCGAG CTGACCCTGC ACTCTGTTTT 
TTGGAGAAGG TGGGGGGACC AGATGAACAG GCTATTGCAG CTGAGCACCA TGCTCAAGAC 
TTTTCTGAAC TCCCTGATGG TGGGGACTTT GATCGAGACA TAGAAGATCC AGAATACAAG 
CCACTTCATG CACAGAAAGA TCCTGAGGAT GAGATCGACT CTTTAATGAT GGATGAAGAA 
ATCTCTGTTG TTGACGGTGA AGAAGCAACA GCAATGCCCA GTGGTATGTT ATGGCCACCT 
GGCTCTGCTC TCACAGCTCG TCTACGGCGT CTAATTACTG CCTACCAACG CAGCTACAAA 
AGAGAACAAC TAAAATTGGA GGCTGAAGAG CGTGGGGACA AACGTAGAAA ACGATGTGAG 
GCAGCCTTTA AACTGAAAGA AATTGCCCGT AGGGAGAAAC AGCAAAGGTG GACCCGAAGG 
GAGGCATGTG ACTTTTTTCG TGTGCTGTCC AGCTTTGGTG TCGAGTATGA CCCCGATACT 
CAACTGTATG ACTGGCAGAG GTTCCGTGGT CTGGCAAGAC TGGACAAGAA GACAGATGAG 
TCACTGCTCA AATACTTCCA TGGATTTGTG GCAATGTGCC GCCAGGTTTG CCGCTTACCC 
CCTGCTGCCG GAGACGAGCC ACCAGATCCA TCTCTCTTCA TTGAGCCAAT TTCAGAAGAG 
CGAGCATCTC GCGCCCTGTT TCGCTTGGAT CTTCTTCGTA GGGTGCGTGA GCAAGTACTT 
TGTCATCCTT TGCTTATTCC TCGTTTGTCA CTGTGCCGGC CAGACCCAGA ACTCCCAGAA 
TGGTGGGAGA GTGGACGTCA TGACAATGAG CTTCTTCAGG GAGCAGCACG TTATGGTCTG 
AGCCGTACAG ACCTCACTAT ACTGCAGGAC CATGCATTTT CTTTTTTGCG GTGTCAGATC 
AGTTATTTCC AGTCAAGGGG AATATCTGGC CATTCTCGCC CTTCTACTCC AACTACTGCC 
CCTTCAGTGC CTACTGAACG TCAGCCTTCT CATCTAGCCT CCCTTTCCTC ATCTGTCAGC 
TGTTCTCCTG CTCCACGCAG ATCCTCTTCC TGCTCATCAT CATCTCACTC ATCAAACTCT 
GAGGATTCTT CAGATGAAAG CAATGGTGTA AAAGTAAAAC CTAATGCAGG TCTTTCGCAC 
GCTCGACTGT ATGATGAGGA GAGCCGGCTT TCTCTTACTG CCTCCCCTGC AGATCTCACT 
ACAGAGGACA GTATTCAGAC AGCGCTGGAG ACCCCGCACA GTACAGACTG GCCAAAGGAC 
CGTGTCCTTA TCTGCAGAAT TGAGCAAGTA TGCAGTGCCG TTCTTACTGG CAAATGGTCC 
TCACCACGAC GACTGTCAGA TCCACCAAGT GACAGCCCTG ATAGCTTGCC TCCAACCCCA 
GAGCAGCAGA GTCCAGCCCA CTTCACACAG ATAAGACCTG CACCGGACCC CAAAGAGTTT 
ACCATTCAAA TAAAAAGTGA AGAAGGCCTG AAGATTAAAT TTCAGAAGCA CAAATTTATG 
GGTAATGGAG CTCTTGATTC TGCACCTCAT ACCCAGAAGA AAAAGAGCAA GAAAAAGATG 
GATCTTGACA TGGACACACG AATTCCCGTT GTAAACCAGA AGGATGGGAC CTTGTTGTTA 
GGGGAAGAAG CCCCTTTACG CTGCCAGCTG CCCGAGTGGT TACACAGAAA TCCTGACTTC 
ATGGTTGACC CACGCTTTAT TGCGTTTATG GAAGACCGCA GGAAAAGAAA AAAACATCGA 
AGCAAAAAGT CCAAATCCTC CTCCCAGATT GGAAACGTAC AGAATCCCCA CTCCCACAAT 
GGAAAGAAGT CTTCCCACAC AGAAAACTCT TTCGAGACGC AACACCTTCC TACTGCAGCC 
AAAAAAAGGA AGAAACGCCC TCCTTCAAAA ATAATAATGC CATCTGCACT ACATGGAGCT 
GGTGATGTTA CACATTCCTT CTCAGACCTT CAACCTGACC ATTCTGGTCA CCCCATGCGG 
TCAGTGTATG GACCATCAGA TGGAGGGCTG TCCCATCAAG CAGGACTCAG CGGGTTGGGA 
TTTACCCGCT TTCATTCATC GCAGACATCT TCAGTGGGCA GAATCCATGG CACTACCTTT 
GATGAGTTAC AAAGTTTTGA GCCCCCACAA CAACATTTTT CTCTGCTTGA TGATCCTATG 
ATGCCAGCAA ATTCCTCCTC CAGCGGTGAT GAACTGGACA GACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chd8 ( XM_012957182.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012812636.1 Xenopus tropicalis chromodomain helicase DNA binding protein 8 (chd8), transcript variant X3, mRNA.

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CloneID	OXa32500
Clone ID Related Accession (Same CDS sequence)	XM_012957172.3 , XM_012957178.3 , XM_031896082.1 , XM_012957178.2 , XM_012957172.2 , XM_012957182.2 , XM_012957182.3
Accession Version	XM_012957182.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7008bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	chromodomain-helicase-DNA-binding protein 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957182.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACC CTATCATGGA CCTCTTTGAT GATCCAAACC TATTTGGCCT TGACTCTCTC 
ACTGGAGATT CCTTTGGACG GGATGGGCCA GACACTATAG ATGATGCTCT AGGCCTGGGT 
AATGTCTTGG GACCTTTGGA GCCGATAACA GACAGAGTTG GCCTGCCTGG TGCTGATGTT 
GGCAATAATG AAGTTAAACA ACAAACAGAG TCACAGGTTC TCCCGGACAA TCCAGCTCTT 
ATGTCAGCTA CTGAGATCAT GCAGCCCATT CAACTTCCCA CAAACCAGGA AACACTGAAC 
CAAGGAAATC CCTTTATGGG TGCCTCAAAT GCTGGAGCCC CAAAAATTAT GATTCTGAAA 
GCTCCTGCTG GAATGACAGT TGGTGCATGT CCTGTAACTC AGATACAAAC TCTGACACCT 
CACCAAGCTG CTAATGGTGG CAAGGTGGCA TTTGCTAAGG TGCTTACAGG AACCCCATTG 
CGTCCTGGGG TCTCAATTGT ATCTGGAAAC ACTGTCCTTG CCAAAATGCC AAGCACTGGA 
GTTGCAGGGC AGGTAGGAGC AGTGAGGCCT GTCCGTCAGC TACTTCTTCA ACCAGTGCGT 
GCCTCCGCAG CCCCAGGAAG CTCCGAGAGT AATACAGGGA TCAAGCCTGC AATCACACTA 
ACGTCAGCAC CTCAACAGGG GGACCCTAAA AGAATTACTC TCGTCCTACA GCAGCCTTCC 
CAAGTTGGTG CAACTGCACA ACAAGGGCAG CGTCATGTTG TGCTGGGTGG GCTTCCAGGA 
AAGATTGTCC TTCAGGGAAA CCAACTGGCA GCTCTCACAC AAGCTAAAAC CCCTCAAGGT 
CAGCCAGCTA AAGTAGTGAC TATCCAGCTG CAAGTCCAGC AGCAGCCTGG GGCTGCAGGT 
CAGACTCCTC AAAAGTTTCA GATTGTACAA CAAGCCCCAG GAGGTGTGGC AATTGCTCCC 
AGTGGGCAAC ATACACACAT GCTTAATCAA CAAGGAGTTC AGAGGCTTTC TGTTCCACTC 
AAAGTTGTTC TGCAACCACA GGCTGGTTCC TCTCAGGGTA CTGCGCAGGG CCTGTCAGTT 
GTGAAAGTTC TAAATGCAAG TGAAGTTGCC AATTTGACTG CAAGTCAGAC TGTGGTTAAA 
ACATCCACTG GAGGTGGTGA ATCTCGTAAG CTGGACAGCC AGAAGAAGCA AGAGAAAGCT 
AACCGTATTG TAGCTGAAGC TATTGCCAGG GCCCGAGCCA GAGGTGAACA GAATATTCCT 
CGGGTCCTGA ATGAGGATGA GCTTCCCAGT GTGAATCCTG AAGATGATGA TGGAAGCAGG 
CGGAAAAGGA AGAAGAAGAA AGGAGAGACT AGTGATAGGT CCAAGGATGA GAAACCTAAG 
AAGGTCAAAG GTAGTGGAAG CTTAAGGTCT AGGAGCAAAG GCAAACCAAG TACTATCACT 
CCTATTGTGG GTAAGAAAAG GAAGAGAAAC CCATCTTCCG ATAATTCTGA TGCTGAGATC 
ATGGCATCAC AGGCTTCTCC TGCAGAGGAT GAAGACAGTG TCCAGAAGAG ACGTTCAAAT 
CGCCAGGTGA AGCGCAAGAA GTATACAGAG GATTTGGATA TTAAAATAAC AGATGATGAA 
GATGATGAGG AGGTAGATGT GACGGGACCT GTGAAAACAG AACCTGTGCT GCTTCCTGAG 
CCCATGCCTT TACCTGACAG TGAGGCAGTC CCTTCAATGC AGTTCTTCGT GGAGAACCCA 
AGTGAAGAAG ATGCTGCTAT TGTTGACAAA ATACTCTCTA TGCGCCTAGC TAAAAAAGAG 
CTGCCCACTG GAGAGTATGT TGAAGTTGAA GAGTACTTTG TCAAATATAA AAATTATTCC 
TACCTACACT GTGAATGGGC CACAATTGAG CAACTGGAAA GGGACAAGCG TATTCATCAG 
AAACTGAAAA GATTTAAGAC AAAGATGACC CAGATGCAGC ATTTCTTGCA AGAAGATGAA 
GAATCTTTCA ATCCAGACTA TGTAGAGGTA GATCGAATCC TAGACGAATC TCACAGCACT 
GACAAGGACA ATGGTGAACC GGTGGTGTAT TACCTAGTAA AATGGTGCTC TTTACCTTAT 
GAAGATAGCA CATGGGAGCT GAAAGAAGAT GTTGATGATG GAAAGATAGA GGAATTCAAA 
CGAATAGAGG CGAGGCAGCC AAATCTCAAA CGCGTGGCCC GCCCAGCTGC TACATCATGG 
AAGAAGCTGG AATTGTCTCG GGAGTATCAG AATGGCAACC AGTTGAGAGA ATACCAGCTG 
GAAGGTGTTA ATTGGCTTCT CTTTAACTGG TACAATAGGC AAAACTGCAT TTTGGCAGAT 
GAAATGGGTT TGGGGAAGAC CATCCAATCT ATCACATTCT TACAAGAAGT ATATAATGTG 
GGAATACGGG GACCTTTTTT AGTAATTGCT CCACTTTCCA CCATCACGAA CTGGGAGAGA 
GAGTTTGGCA GTTGGACTCA AATGAATACT ATTGTGTATC ATGGCAGCCT TGCTAGTCGC 
CAAATGATCC AGCAATATGA GATGTACTGT AAGGACTCCA AGGGTCGTCT TATTCCTGGT 
GCGTACAAGT TTGATGCTCT TATTACAACT TTTGAAATGG TGCTGTCTGA TTGCCCAGAG 
CTGCGTGAAA TTGAGTGGCG CTGTGTCATA ATTGATGAGG CTCATCGGCT CAAAAACAGA 
AACTGCAAAC TACTGGACAG CCTAAAGCAC ATGGACTTGG AGCACAAAGT CCTTTTAACA 
GGCACTCCAT TACAGAACAC CGTGGAAGAG TTGTTCAGTC TTTTGCATTT CCTGGAGCCA 
ACACAATTTT CCTCTGAAGC AGAGTTTCTT AAGGACTTTG GAGACCTGAA GACAGAGGAA 
CAGGTGCAGA AATTACAGGC CATCTTAAAA CCTATGATGT TGAGGCGATT AAAGGAAGAT 
GTTGAGAAAA ACCTGGCCCC GAAACAAGAA ACTATAATTG AGGTTGAGCT TACTAACATC 
CAGAAGAAGT ATTACCGAGC AATACTGGAG AAGAATTTTT CTTTTCTGAC CAAAGGAGCT 
AGTCAATCCA ACACACCCAA CTTGCTCAAT ACCATGATGG AACTACGCAA ATGCTGCAAC 
CACCCATACC TCATCACTGG AGCAGAAGAA AAGATCATAT CTGAGTTCCG AGAAGCAACT 
CCAGTAGTTC CCCCTGACTT TCACGTCCAA GCAATGGTAC GCTCCTCTGG CAAACTGGTG 
CTAATAGATA AGCTGCTCCC TAAACTAAGG GCTGGAGGCC ACAAAGTTCT CATCTTTTCT 
CAGATGGTAC GGTGCCTGGA CATCCTGGAA GACTATCTCA TTCAGAGAAG GTACCTTTAT 
GAGCGTATTG ATGGACGTGT CCGTGGCAAC ATGCGTCAGG CAGCCATTGA TAGATTCAGC 
CGCCCAGATT CTGACCGATT TGTGTTTCTA CTCTGTACAC GAGCTGGTGG GCTTGGAATT 
AACTTGACAG CTGCTGATAC TTGTATTATA TTTGACTCGG ACTGGAACCC TCAAAATGAC 
CTTCAGGCTC AAGCTCGATG TCATCGTATT GGGCAAAGCA AAGCCGTTAA AATTTACAGA 
CTCATCACTC GCAACTCCTA TGAGAGAGAG ATGTTTGATA AGGCCAGTCT CAAACTAGGA 
CTAGACAAAG CTGTATTGCA GTCTATGAGT GGTCGGGATA ATCACCTTAG TGGGCCTATT 
CAGCAATTCA CAAAGAAAGA AATTGAAGAC TTGCTAAGGA AAGGAGCTTA TGCTGCAATC 
ATGGATGAAG ATGACGAAGG CTCTAAATTC TGTGAAGAAG ATATTGACCA AATTTTACTG 
AGGCGTACCA CGACTATTAC CATAGAGTCA GAGGGAAAGG GCTCAACCTT CTCCAAGGCA 
AGTTTTGTTG CTTCTGAGAA CAGGACTGAT ATATCCCTAG ATGATCCTAA TTTCTGGCAA 
AAGTGGGCCA AGAAAGCAGA CTTAGACTTG GATCTTCTTA GCAGCAAGAA TACACTGGTT 
ATTGATACCC CACGTATCAG GAAGCAAACA CGGCACTTTA CTAACAAAGA TGATGACATG 
GTTGAGTTCT CAGATTTGGA GAGTGATGAT GATGATCGCC CCAAAGCTCG GCGTCAAGAC 
CGCAAGCATG GATATGGACG TACAGATTGC TTCCGTGTGG AGAAGCATTT GCTTGTTTAT 
GGATGGGGTC GCTGGCGAGA TATCCTTACT CATGGTCGTT TCAAGCGTGG TATGAATGAG 
CGTGACGTGG AGAAAATATG TAGAGCCATA CTGGTTTATT GTCTTTTGCA CTACCGGGGA 
GATGAAAACA TAAAGAGCTT TATCTGGGAT CTAATTACAC CAGCTGAGAA TGGCAAAACT 
AAGCAGCTAC AGAACCACTC TGGATTGTCT ATTCCTGTCC CCCGTGGTCG TAAGGGGAAA 
AAAGTGAAAT CCCAAAGCAG CTTTGACATA CATAAAGCCG ATTGGATACA TAAGTACAAC 
CCAGATACCT TGTTCCAAGA TGAAGGGTAC AAGAAACATC TCAAGCATCA ATGTAACAAG 
GTGCTGCTTC GCGTTCGTAT GTTATATTTT CTTCGTCAGG AAGTTATTGG AAACCAGGCA 
ATGAAAGTTT TAAGTGGTGT AGAGGCCAGG CAAATTGACA TCTGGTTCCC ACAGGTGGAC 
CAGGTTGAAG TTCCCTCACG CTGGTGGGAC AGAGAAGCTG ATAAATCTCT TCTTATCGGG 
GTCTTTAAAC ATGGTTATGA GAAGTATAAC ACCATGCGAG CTGACCCTGC ACTCTGTTTT 
TTGGAGAAGG TGGGGGGACC AGATGAACAG GCTATTGCAG CTGAGCACCA TGCTCAAGAC 
TTTTCTGAAC TCCCTGATGG TGGGGACTTT GATCGAGACA TAGAAGATCC AGAATACAAG 
CCACTTCATG CACAGAAAGA TCCTGAGGAT GAGATCGACT CTTTAATGAT GGATGAAGAA 
ATCTCTGTTG TTGACGGTGA AGAAGCAACA GCAATGCCCA GTGGTATGTT ATGGCCACCT 
GGCTCTGCTC TCACAGCTCG TCTACGGCGT CTAATTACTG CCTACCAACG CAGCTACAAA 
AGAGAACAAC TAAAATTGGA GGCTGAAGAG CGTGGGGACA AACGTAGAAA ACGATGTGAG 
GCAGCCTTTA AACTGAAAGA AATTGCCCGT AGGGAGAAAC AGCAAAGGTG GACCCGAAGG 
GAGGCATGTG ACTTTTTTCG TGTGCTGTCC AGCTTTGGTG TCGAGTATGA CCCCGATACT 
CAACTGTATG ACTGGCAGAG GTTCCGTGGT CTGGCAAGAC TGGACAAGAA GACAGATGAG 
TCACTGCTCA AATACTTCCA TGGATTTGTG GCAATGTGCC GCCAGGTTTG CCGCTTACCC 
CCTGCTGCCG GAGACGAGCC ACCAGATCCA TCTCTCTTCA TTGAGCCAAT TTCAGAAGAG 
CGAGCATCTC GCGCCCTGTT TCGCTTGGAT CTTCTTCGTA GGGTGCGTGA GCAAGTACTT 
TGTCATCCTT TGCTTATTCC TCGTTTGTCA CTGTGCCGGC CAGACCCAGA ACTCCCAGAA 
TGGTGGGAGA GTGGACGTCA TGACAATGAG CTTCTTCAGG GAGCAGCACG TTATGGTCTG 
AGCCGTACAG ACCTCACTAT ACTGCAGGAC CATGCATTTT CTTTTTTGCG GTGTCAGATC 
AGTTATTTCC AGTCAAGGGG AATATCTGGC CATTCTCGCC CTTCTACTCC AACTACTGCC 
CCTTCAGTGC CTACTGAACG TCAGCCTTCT CATCTAGCCT CCCTTTCCTC ATCTGTCAGC 
TGTTCTCCTG CTCCACGCAG ATCCTCTTCC TGCTCATCAT CATCTCACTC ATCAAACTCT 
GAGGATTCTT CAGATGAAAG CAATGGTGTA AAAGTAAAAC CTAATGCAGG TCTTTCGCAC 
GCTCGACTGT ATGATGAGGA GAGCCGGCTT TCTCTTACTG CCTCCCCTGC AGATCTCACT 
ACAGAGGACA GTATTCAGAC AGCGCTGGAG ACCCCGCACA GTACAGACTG GCCAAAGGAC 
CGTGTCCTTA TCTGCAGAAT TGAGCAAGTA TGCAGTGCCG TTCTTACTGG CAAATGGTCC 
TCACCACGAC GACTGTCAGA TCCACCAAGT GACAGCCCTG ATAGCTTGCC TCCAACCCCA 
GAGCAGCAGA GTCCAGCCCA CTTCACACAG ATAAGACCTG CACCGGACCC CAAAGAGTTT 
ACCATTCAAA TAAAAAGTGA AGAAGGCCTG AAGATTAAAT TTCAGAAGCA CAAATTTATG 
GGTAATGGAG CTCTTGATTC TGCACCTCAT ACCCAGAAGA AAAAGAGCAA GAAAAAGATG 
GATCTTGACA TGGACACACG AATTCCCGTT GTAAACCAGA AGGATGGGAC CTTGTTGTTA 
GGGGAAGAAG CCCCTTTACG CTGCCAGCTG CCCGAGTGGT TACACAGAAA TCCTGACTTC 
ATGGTTGACC CACGCTTTAT TGCGTTTATG GAAGACCGCA GGAAAAGAAA AAAACATCGA 
AGCAAAAAGT CCAAATCCTC CTCCCAGATT GGAAACGTAC AGAATCCCCA CTCCCACAAT 
GGAAAGAAGT CTTCCCACAC AGAAAACTCT TTCGAGACGC AACACCTTCC TACTGCAGCC 
AAAAAAAGGA AGAAACGCCC TCCTTCAAAA ATAATAATGC CATCTGCACT ACATGGAGCT 
GGTGATGTTA CACATTCCTT CTCAGACCTT CAACCTGACC ATTCTGGTCA CCCCATGCGG 
TCAGTGTATG GACCATCAGA TGGAGGGCTG TCCCATCAAG CAGGACTCAG CGGGTTGGGA 
TTTACCCGCT TTCATTCATC GCAGACATCT TCAGTGGGCA GAATCCATGG CACTACCTTT 
GATGAGTTAC AAAGTTTTGA GCCCCCACAA CAACATTTTT CTCTGCTTGA TGATCCTATG 
ATGCCAGCAA ATTCCTCCTC CAGCGGTGAT GAACTGGACA GACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012812636.1
CDS	253..7260
Translation	MADPIMDLFDDPNLFGLDSLTGDSFGRDGPDTIDDALGLGNVLGPLEPITDRVGLPGADVGNNEVKQQTESQVLPDNPALMSATEIMQPIQLPTNQETLNQGNPFMGASNAGAPKIMILKAPAGMTVGACPVTQIQTLTPHQAANGGKVAFAKVLTGTPLRPGVSIVSGNTVLAKMPSTGVAGQVGAVRPVRQLLLQPVRASAAPGSSESNTGIKPAITLTSAPQQGDPKRITLVLQQPSQVGATAQQGQRHVVLGGLPGKIVLQGNQLAALTQAKTPQGQPAKVVTIQLQVQQQPGAAGQTPQKFQIVQQAPGGVAIAPSGQHTHMLNQQGVQRLSVPLKVVLQPQAGSSQGTAQGLSVVKVLNASEVANLTASQTVVKTSTGGGESRKLDSQKKQEKANRIVAEAIARARARGEQNIPRVLNEDELPSVNPEDDDGSRRKRKKKKGETSDRSKDEKPKKVKGSGSLRSRSKGKPSTITPIVGKKRKRNPSSDNSDAEIMASQASPAEDEDSVQKRRSNRQVKRKKYTEDLDIKITDDEDDEEVDVTGPVKTEPVLLPEPMPLPDSEAVPSMQFFVENPSEEDAAIVDKILSMRLAKKELPTGEYVEVEEYFVKYKNYSYLHCEWATIEQLERDKRIHQKLKRFKTKMTQMQHFLQEDEESFNPDYVEVDRILDESHSTDKDNGEPVVYYLVKWCSLPYEDSTWELKEDVDDGKIEEFKRIEARQPNLKRVARPAATSWKKLELSREYQNGNQLREYQLEGVNWLLFNWYNRQNCILADEMGLGKTIQSITFLQEVYNVGIRGPFLVIAPLSTITNWEREFGSWTQMNTIVYHGSLASRQMIQQYEMYCKDSKGRLIPGAYKFDALITTFEMVLSDCPELREIEWRCVIIDEAHRLKNRNCKLLDSLKHMDLEHKVLLTGTPLQNTVEELFSLLHFLEPTQFSSEAEFLKDFGDLKTEEQVQKLQAILKPMMLRRLKEDVEKNLAPKQETIIEVELTNIQKKYYRAILEKNFSFLTKGASQSNTPNLLNTMMELRKCCNHPYLITGAEEKIISEFREATPVVPPDFHVQAMVRSSGKLVLIDKLLPKLRAGGHKVLIFSQMVRCLDILEDYLIQRRYLYERIDGRVRGNMRQAAIDRFSRPDSDRFVFLLCTRAGGLGINLTAADTCIIFDSDWNPQNDLQAQARCHRIGQSKAVKIYRLITRNSYEREMFDKASLKLGLDKAVLQSMSGRDNHLSGPIQQFTKKEIEDLLRKGAYAAIMDEDDEGSKFCEEDIDQILLRRTTTITIESEGKGSTFSKASFVASENRTDISLDDPNFWQKWAKKADLDLDLLSSKNTLVIDTPRIRKQTRHFTNKDDDMVEFSDLESDDDDRPKARRQDRKHGYGRTDCFRVEKHLLVYGWGRWRDILTHGRFKRGMNERDVEKICRAILVYCLLHYRGDENIKSFIWDLITPAENGKTKQLQNHSGLSIPVPRGRKGKKVKSQSSFDIHKADWIHKYNPDTLFQDEGYKKHLKHQCNKVLLRVRMLYFLRQEVIGNQAMKVLSGVEARQIDIWFPQVDQVEVPSRWWDREADKSLLIGVFKHGYEKYNTMRADPALCFLEKVGGPDEQAIAAEHHAQDFSELPDGGDFDRDIEDPEYKPLHAQKDPEDEIDSLMMDEEISVVDGEEATAMPSGMLWPPGSALTARLRRLITAYQRSYKREQLKLEAEERGDKRRKRCEAAFKLKEIARREKQQRWTRREACDFFRVLSSFGVEYDPDTQLYDWQRFRGLARLDKKTDESLLKYFHGFVAMCRQVCRLPPAAGDEPPDPSLFIEPISEERASRALFRLDLLRRVREQVLCHPLLIPRLSLCRPDPELPEWWESGRHDNELLQGAARYGLSRTDLTILQDHAFSFLRCQISYFQSRGISGHSRPSTPTTAPSVPTERQPSHLASLSSSVSCSPAPRRSSSCSSSSHSSNSEDSSDESNGVKVKPNAGLSHARLYDEESRLSLTASPADLTTEDSIQTALETPHSTDWPKDRVLICRIEQVCSAVLTGKWSSPRRLSDPPSDSPDSLPPTPEQQSPAHFTQIRPAPDPKEFTIQIKSEEGLKIKFQKHKFMGNGALDSAPHTQKKKSKKKMDLDMDTRIPVVNQKDGTLLLGEEAPLRCQLPEWLHRNPDFMVDPRFIAFMEDRRKRKKHRSKKSKSSSQIGNVQNPHSHNGKKSSHTENSFETQHLPTAAKKRKKRPPSKIIMPSALHGAGDVTHSFSDLQPDHSGHPMRSVYGPSDGGLSHQAGLSGLGFTRFHSSQTSSVGRIHGTTFDELQSFEPPQQHFSLLDDPMMPANSSSSGDELDRP

Target ORF information:

RefSeq Version	XM_012957182.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis chromodomain helicase DNA binding protein 8 (chd8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957182.2

ORF Insert Sequence:

ATGGCTGACC CTATCATGGA CCTCTTTGAT GATCCAAACC TATTTGGCCT TGACTCTCTC 
ACTGGAGATT CCTTTGGACG GGATGGGCCA GACACTATAG ATGATGCTCT AGGCCTGGGT 
AATGTCTTGG GACCTTTGGA GCCGATAACA GACAGAGTTG GCCTGCCTGG TGCTGATGTT 
GGCAATAATG AAGTTAAACA ACAAACAGAG TCACAGGTTC TCCCGGACAA TCCAGCTCTT 
ATGTCAGCTA CTGAGATCAT GCAGCCCATT CAACTTCCCA CAAACCAGGA AACACTGAAC 
CAAGGAAATC CCTTTATGGG TGCCTCAAAT GCTGGAGCCC CAAAAATTAT GATTCTGAAA 
GCTCCTGCTG GAATGACAGT TGGTGCATGT CCTGTAACTC AGATACAAAC TCTGACACCT 
CACCAAGCTG CTAATGGTGG CAAGGTGGCA TTTGCTAAGG TGCTTACAGG AACCCCATTG 
CGTCCTGGGG TCTCAATTGT ATCTGGAAAC ACTGTCCTTG CCAAAATGCC AAGCACTGGA 
GTTGCAGGGC AGGTAGGAGC AGTGAGGCCT GTCCGTCAGC TACTTCTTCA ACCAGTGCGT 
GCCTCCGCAG CCCCAGGAAG CTCCGAGAGT AATACAGGGA TCAAGCCTGC AATCACACTA 
ACGTCAGCAC CTCAACAGGG GGACCCTAAA AGAATTACTC TCGTCCTACA GCAGCCTTCC 
CAAGTTGGTG CAACTGCACA ACAAGGGCAG CGTCATGTTG TGCTGGGTGG GCTTCCAGGA 
AAGATTGTCC TTCAGGGAAA CCAACTGGCA GCTCTCACAC AAGCTAAAAC CCCTCAAGGT 
CAGCCAGCTA AAGTAGTGAC TATCCAGCTG CAAGTCCAGC AGCAGCCTGG GGCTGCAGGT 
CAGACTCCTC AAAAGTTTCA GATTGTACAA CAAGCCCCAG GAGGTGTGGC AATTGCTCCC 
AGTGGGCAAC ATACACACAT GCTTAATCAA CAAGGAGTTC AGAGGCTTTC TGTTCCACTC 
AAAGTTGTTC TGCAACCACA GGCTGGTTCC TCTCAGGGTA CTGCGCAGGG CCTGTCAGTT 
GTGAAAGTTC TAAATGCAAG TGAAGTTGCC AATTTGACTG CAAGTCAGAC TGTGGTTAAA 
ACATCCACTG GAGGTGGTGA ATCTCGTAAG CTGGACAGCC AGAAGAAGCA AGAGAAAGCT 
AACCGTATTG TAGCTGAAGC TATTGCCAGG GCCCGAGCCA GAGGTGAACA GAATATTCCT 
CGGGTCCTGA ATGAGGATGA GCTTCCCAGT GTGAATCCTG AAGATGATGA TGGAAGCAGG 
CGGAAAAGGA AGAAGAAGAA AGGAGAGACT AGTGATAGGT CCAAGGATGA GAAACCTAAG 
AAGGTCAAAG GTAGTGGAAG CTTAAGGTCT AGGAGCAAAG GCAAACCAAG TACTATCACT 
CCTATTGTGG GTAAGAAAAG GAAGAGAAAC CCATCTTCCG ATAATTCTGA TGCTGAGATC 
ATGGCATCAC AGGCTTCTCC TGCAGAGGAT GAAGACAGTG TCCAGAAGAG ACGTTCAAAT 
CGCCAGGTGA AGCGCAAGAA GTATACAGAG GATTTGGATA TTAAAATAAC AGATGATGAA 
GATGATGAGG AGGTAGATGT GACGGGACCT GTGAAAACAG AACCTGTGCT GCTTCCTGAG 
CCCATGCCTT TACCTGACAG TGAGGCAGTC CCTTCAATGC AGTTCTTCGT GGAGAACCCA 
AGTGAAGAAG ATGCTGCTAT TGTTGACAAA ATACTCTCTA TGCGCCTAGC TAAAAAAGAG 
CTGCCCACTG GAGAGTATGT TGAAGTTGAA GAGTACTTTG TCAAATATAA AAATTATTCC 
TACCTACACT GTGAATGGGC CACAATTGAG CAACTGGAAA GGGACAAGCG TATTCATCAG 
AAACTGAAAA GATTTAAGAC AAAGATGACC CAGATGCAGC ATTTCTTGCA AGAAGATGAA 
GAATCTTTCA ATCCAGACTA TGTAGAGGTA GATCGAATCC TAGACGAATC TCACAGCACT 
GACAAGGACA ATGGTGAACC GGTGGTGTAT TACCTAGTAA AATGGTGCTC TTTACCTTAT 
GAAGATAGCA CATGGGAGCT GAAAGAAGAT GTTGATGATG GAAAGATAGA GGAATTCAAA 
CGAATAGAGG CGAGGCAGCC AAATCTCAAA CGCGTGGCCC GCCCAGCTGC TACATCATGG 
AAGAAGCTGG AATTGTCTCG GGAGTATCAG AATGGCAACC AGTTGAGAGA ATACCAGCTG 
GAAGGTGTTA ATTGGCTTCT CTTTAACTGG TACAATAGGC AAAACTGCAT TTTGGCAGAT 
GAAATGGGTT TGGGGAAGAC CATCCAATCT ATCACATTCT TACAAGAAGT ATATAATGTG 
GGAATACGGG GACCTTTTTT AGTAATTGCT CCACTTTCCA CCATCACGAA CTGGGAGAGA 
GAGTTTGGCA GTTGGACTCA AATGAATACT ATTGTGTATC ATGGCAGCCT TGCTAGTCGC 
CAAATGATCC AGCAATATGA GATGTACTGT AAGGACTCCA AGGGTCGTCT TATTCCTGGT 
GCGTACAAGT TTGATGCTCT TATTACAACT TTTGAAATGG TGCTGTCTGA TTGCCCAGAG 
CTGCGTGAAA TTGAGTGGCG CTGTGTCATA ATTGATGAGG CTCATCGGCT CAAAAACAGA 
AACTGCAAAC TACTGGACAG CCTAAAGCAC ATGGACTTGG AGCACAAAGT CCTTTTAACA 
GGCACTCCAT TACAGAACAC CGTGGAAGAG TTGTTCAGTC TTTTGCATTT CCTGGAGCCA 
ACACAATTTT CCTCTGAAGC AGAGTTTCTT AAGGACTTTG GAGACCTGAA GACAGAGGAA 
CAGGTGCAGA AATTACAGGC CATCTTAAAA CCTATGATGT TGAGGCGATT AAAGGAAGAT 
GTTGAGAAAA ACCTGGCCCC GAAACAAGAA ACTATAATTG AGGTTGAGCT TACTAACATC 
CAGAAGAAGT ATTACCGAGC AATACTGGAG AAGAATTTTT CTTTTCTGAC CAAAGGAGCT 
AGTCAATCCA ACACACCCAA CTTGCTCAAT ACCATGATGG AACTACGCAA ATGCTGCAAC 
CACCCATACC TCATCACTGG AGCAGAAGAA AAGATCATAT CTGAGTTCCG AGAAGCAACT 
CCAGTAGTTC CCCCTGACTT TCACGTCCAA GCAATGGTAC GCTCCTCTGG CAAACTGGTG 
CTAATAGATA AGCTGCTCCC TAAACTAAGG GCTGGAGGCC ACAAAGTTCT CATCTTTTCT 
CAGATGGTAC GGTGCCTGGA CATCCTGGAA GACTATCTCA TTCAGAGAAG GTACCTTTAT 
GAGCGTATTG ATGGACGTGT CCGTGGCAAC ATGCGTCAGG CAGCCATTGA TAGATTCAGC 
CGCCCAGATT CTGACCGATT TGTGTTTCTA CTCTGTACAC GAGCTGGTGG GCTTGGAATT 
AACTTGACAG CTGCTGATAC TTGTATTATA TTTGACTCGG ACTGGAACCC TCAAAATGAC 
CTTCAGGCTC AAGCTCGATG TCATCGTATT GGGCAAAGCA AAGCCGTTAA AATTTACAGA 
CTCATCACTC GCAACTCCTA TGAGAGAGAG ATGTTTGATA AGGCCAGTCT CAAACTAGGA 
CTAGACAAAG CTGTATTGCA GTCTATGAGT GGTCGGGATA ATCACCTTAG TGGGCCTATT 
CAGCAATTCA CAAAGAAAGA AATTGAAGAC TTGCTAAGGA AAGGAGCTTA TGCTGCAATC 
ATGGATGAAG ATGACGAAGG CTCTAAATTC TGTGAAGAAG ATATTGACCA AATTTTACTG 
AGGCGTACCA CGACTATTAC CATAGAGTCA GAGGGAAAGG GCTCAACCTT CTCCAAGGCA 
AGTTTTGTTG CTTCTGAGAA CAGGACTGAT ATATCCCTAG ATGATCCTAA TTTCTGGCAA 
AAGTGGGCCA AGAAAGCAGA CTTAGACTTG GATCTTCTTA GCAGCAAGAA TACACTGGTT 
ATTGATACCC CACGTATCAG GAAGCAAACA CGGCACTTTA CTAACAAAGA TGATGACATG 
GTTGAGTTCT CAGATTTGGA GAGTGATGAT GATGATCGCC CCAAAGCTCG GCGTCAAGAC 
CGCAAGCATG GATATGGACG TACAGATTGC TTCCGTGTGG AGAAGCATTT GCTTGTTTAT 
GGATGGGGTC GCTGGCGAGA TATCCTTACT CATGGTCGTT TCAAGCGTGG TATGAATGAG 
CGTGACGTGG AGAAAATATG TAGAGCCATA CTGGTTTATT GTCTTTTGCA CTACCGGGGA 
GATGAAAACA TAAAGAGCTT TATCTGGGAT CTAATTACAC CAGCTGAGAA TGGCAAAACT 
AAGCAGCTAC AGAACCACTC TGGATTGTCT ATTCCTGTCC CCCGTGGTCG TAAGGGGAAA 
AAAGTGAAAT CCCAAAGCAG CTTTGACATA CATAAAGCCG ATTGGATACA TAAGTACAAC 
CCAGATACCT TGTTCCAAGA TGAAGGGTAC AAGAAACATC TCAAGCATCA ATGTAACAAG 
GTGCTGCTTC GCGTTCGTAT GTTATATTTT CTTCGTCAGG AAGTTATTGG AAACCAGGCA 
ATGAAAGTTT TAAGTGGTGT AGAGGCCAGG CAAATTGACA TCTGGTTCCC ACAGGTGGAC 
CAGGTTGAAG TTCCCTCACG CTGGTGGGAC AGAGAAGCTG ATAAATCTCT TCTTATCGGG 
GTCTTTAAAC ATGGTTATGA GAAGTATAAC ACCATGCGAG CTGACCCTGC ACTCTGTTTT 
TTGGAGAAGG TGGGGGGACC AGATGAACAG GCTATTGCAG CTGAGCACCA TGCTCAAGAC 
TTTTCTGAAC TCCCTGATGG TGGGGACTTT GATCGAGACA TAGAAGATCC AGAATACAAG 
CCACTTCATG CACAGAAAGA TCCTGAGGAT GAGATCGACT CTTTAATGAT GGATGAAGAA 
ATCTCTGTTG TTGACGGTGA AGAAGCAACA GCAATGCCCA GTGGTATGTT ATGGCCACCT 
GGCTCTGCTC TCACAGCTCG TCTACGGCGT CTAATTACTG CCTACCAACG CAGCTACAAA 
AGAGAACAAC TAAAATTGGA GGCTGAAGAG CGTGGGGACA AACGTAGAAA ACGATGTGAG 
GCAGCCTTTA AACTGAAAGA AATTGCCCGT AGGGAGAAAC AGCAAAGGTG GACCCGAAGG 
GAGGCATGTG ACTTTTTTCG TGTGCTGTCC AGCTTTGGTG TCGAGTATGA CCCCGATACT 
CAACTGTATG ACTGGCAGAG GTTCCGTGGT CTGGCAAGAC TGGACAAGAA GACAGATGAG 
TCACTGCTCA AATACTTCCA TGGATTTGTG GCAATGTGCC GCCAGGTTTG CCGCTTACCC 
CCTGCTGCCG GAGACGAGCC ACCAGATCCA TCTCTCTTCA TTGAGCCAAT TTCAGAAGAG 
CGAGCATCTC GCGCCCTGTT TCGCTTGGAT CTTCTTCGTA GGGTGCGTGA GCAAGTACTT 
TGTCATCCTT TGCTTATTCC TCGTTTGTCA CTGTGCCGGC CAGACCCAGA ACTCCCAGAA 
TGGTGGGAGA GTGGACGTCA TGACAATGAG CTTCTTCAGG GAGCAGCACG TTATGGTCTG 
AGCCGTACAG ACCTCACTAT ACTGCAGGAC CATGCATTTT CTTTTTTGCG GTGTCAGATC 
AGTTATTTCC AGTCAAGGGG AATATCTGGC CATTCTCGCC CTTCTACTCC AACTACTGCC 
CCTTCAGTGC CTACTGAACG TCAGCCTTCT CATCTAGCCT CCCTTTCCTC ATCTGTCAGC 
TGTTCTCCTG CTCCACGCAG ATCCTCTTCC TGCTCATCAT CATCTCACTC ATCAAACTCT 
GAGGATTCTT CAGATGAAAG CAATGGTGTA AAAGTAAAAC CTAATGCAGG TCTTTCGCAC 
GCTCGACTGT ATGATGAGGA GAGCCGGCTT TCTCTTACTG CCTCCCCTGC AGATCTCACT 
ACAGAGGACA GTATTCAGAC AGCGCTGGAG ACCCCGCACA GTACAGACTG GCCAAAGGAC 
CGTGTCCTTA TCTGCAGAAT TGAGCAAGTA TGCAGTGCCG TTCTTACTGG CAAATGGTCC 
TCACCACGAC GACTGTCAGA TCCACCAAGT GACAGCCCTG ATAGCTTGCC TCCAACCCCA 
GAGCAGCAGA GTCCAGCCCA CTTCACACAG ATAAGACCTG CACCGGACCC CAAAGAGTTT 
ACCATTCAAA TAAAAAGTGA AGAAGGCCTG AAGATTAAAT TTCAGAAGCA CAAATTTATG 
GGTAATGGAG CTCTTGATTC TGCACCTCAT ACCCAGAAGA AAAAGAGCAA GAAAAAGATG 
GATCTTGACA TGGACACACG AATTCCCGTT GTAAACCAGA AGGATGGGAC CTTGTTGTTA 
GGGGAAGAAG CCCCTTTACG CTGCCAGCTG CCCGAGTGGT TACACAGAAA TCCTGACTTC 
ATGGTTGACC CACGCTTTAT TGCGTTTATG GAAGACCGCA GGAAAAGAAA AAAACATCGA 
AGCAAAAAGT CCAAATCCTC CTCCCAGATT GGAAACGTAC AGAATCCCCA CTCCCACAAT 
GGAAAGAAGT CTTCCCACAC AGAAAACTCT TTCGAGACGC AACACCTTCC TACTGCAGCC 
AAAAAAAGGA AGAAACGCCC TCCTTCAAAA ATAATAATGC CATCTGCACT ACATGGAGCT 
GGTGATGTTA CACATTCCTT CTCAGACCTT CAACCTGACC ATTCTGGTCA CCCCATGCGG 
TCAGTGTATG GACCATCAGA TGGAGGGCTG TCCCATCAAG CAGGACTCAG CGGGTTGGGA 
TTTACCCGCT TTCATTCATC GCAGACATCT TCAGTGGGCA GAATCCATGG CACTACCTTT 
GATGAGTTAC AAAGTTTTGA GCCCCCACAA CAACATTTTT CTCTGCTTGA TGATCCTATG 
ATGCCAGCAA ATTCCTCCTC CAGCGGTGAT GAACTGGACA GACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chd8 ( XM_012957198.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012812652.1 Xenopus tropicalis chromodomain helicase DNA binding protein 8 (chd8), transcript variant X4, mRNA.

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CloneID	OXa32501
Clone ID Related Accession (Same CDS sequence)	XM_012957198.3 , XM_012957198.2
Accession Version	XM_012957198.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7005bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	chromodomain-helicase-DNA-binding protein 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957198.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACC CTATCATGGA CCTCTTTGAT GATCCAAACC TATTTGGCCT TGACTCTCTC 
ACTGGAGATT CCTTTGGACG GGATGGGCCA GACACTATAG ATGATGCTCT AGGCCTGGGT 
AATGTCTTGG GACCTTTGGA GCCGATAACA GACAGAGTTG GCCTGCCTGG TGCTGATGTT 
GGCAATAATG AAGTTAAACA ACAAACAGAG TCACAGGTTC TCCCGGACAA TCCAGCTCTT 
ATGTCAGCTA CTGAGATCAT GCAGCCCATT CAACTTCCCA CAAACCAGGA AACACTGAAC 
CAAGGAAATC CCTTTATGGG TGCCTCAAAT GCTGGAGCCC CAAAAATTAT GATTCTGAAA 
GCTCCTGCTG GAATGACAGT TGGTGCATGT CCTGTAACTC AGATACAAAC TCTGACACCT 
CACCAAGCTG CTAATGGTGG CAAGGTGGCA TTTGCTAAGG TGCTTACAGG AACCCCATTG 
CGTCCTGGGG TCTCAATTGT ATCTGGAAAC ACTGTCCTTG CCAAAATGCC AAGCACTGGA 
GTTGCAGGGC AGGTAGGAGC AGTGAGGCCT GTCCGTCAGC TACTTCTTCA ACCAGTGCGT 
GCCTCCGCAG CCCCAGGAAG CTCCGAGAGT AATACAGGGA TCAAGCCTGC AATCACACTA 
ACGTCAGCAC CTCAACAGGG GGACCCTAAA AGAATTACTC TCGTCCTACA GCAGCCTTCC 
CAAGTTGGTG CAACTGCACA ACAAGGGCAG CGTCATGTTG TGCTGGGTGG GCTTCCAGGA 
AAGATTGTCC TTCAGGGAAA CCAACTGGCA GCTCTCACAC AAGCTAAAAC CCCTCAAGGT 
CAGCCAGCTA AAGTAGTGAC TATCCAGCTG CAAGTCCAGC AGCAGCCTGG GGCTGCAGGT 
CAGACTCCTC AAAAGTTTCA GATTGTACAA CAAGCCCCAG GAGGTGTGGC AATTGCTCCC 
AGTGGGCAAC ATACACACAT GCTTAATCAA CAAGGAGTTC AGAGGCTTTC TGTTCCACTC 
AAAGTTGTTC TGCAACCACA GGCTGGTTCC TCTCAGGGTA CTGCGCAGGG CCTGTCAGTT 
GTGAAAGTTC TAAATGCAAG TGAAGTTGCC AATTTGACTG CAAGTCAGAC TGTGGTTAAA 
ACATCCACTG GAGGTGGTGA ATCTCGTAAG CTGGACAGCC AGAAGAAGCA AGAGAAAGCT 
AACCGTATTG TAGCTGAAGC TATTGCCAGG GCCCGAGCCA GAGGTGAACA GAATATTCCT 
CGGGTCCTGA ATGAGGATGA GCTTCCCAGT GTGAATCCTG AAGATGATGA TGGAAGCAGG 
CGGAAAAGGA AGAAGAAGAA AGGAGAGACT AGTGATAGGT CCAAGGATGA GAAACCTAAG 
AAGGTCAAAG GTAGTGGAAG CTTAAGGTCT AGGAGCAAAG GCAAACCAAG TACTATCACT 
CCTATTGTGG GTAAGAAAAG GAAGAGAAAC CCATCTTCCG ATAATTCTGA TGCTGAGATC 
ATGGCATCAC AGGCTTCTCC TGCAGAGGAT GAAGACAGTG TCCAGAAGAG ACGTTCAAAT 
CGCCAGGTGA AGCGCAAGAA GTATACAGAG GATTTGGATA TTAAAATAAC AGATGATGAA 
GATGATGAGG AGGTAGATGT GACGGGACCT GTGAAAACAG AACCTGTGCT GCTTCCTGAG 
CCCATGCCTT TACCTGACAG TGAGGCAGTC CCTTCAATGC AGTTCTTCGT GGAGAACCCA 
AGTGAAGAAG ATGCTGCTAT TGTTGACAAA ATACTCTCTA TGCGCCTAGC TAAAAAAGAG 
CTGCCCACTG GAGAGTATGT TGAAGTTGAA GAGTACTTTG TCAAATATAA AAATTATTCC 
TACCTACACT GTGAATGGGC CACAATTGAG CAACTGGAAA GGGACAAGCG TATTCATCAG 
AAACTGAAAA GATTTAAGAC AAAGATGACC CAGATGCAGC ATTTCTTGCA AGAAGATGAA 
GAATCTTTCA ATCCAGACTA TGTAGAGGTA GATCGAATCC TAGACGAATC TCACAGCACT 
GACAAGGACA ATGGTGAACC GGTGGTGTAT TACCTAGTAA AATGGTGCTC TTTACCTTAT 
GAAGATAGCA CATGGGAGCT GAAAGAAGAT GTTGATGATG GAAAGATAGA GGAATTCAAA 
CGAATAGAGG CGAGGCAGCC AAATCTCAAA CGCGTGGCCC GCCCAGCTGC TACATCATGG 
AAGAAGCTGG AATTGTCTCG GGAGTATCAG AATGGCAACC AGTTGAGAGA ATACCAGCTG 
GAAGGTGTTA ATTGGCTTCT CTTTAACTGG TACAATAGGC AAAACTGCAT TTTGGCAGAT 
GAAATGGGTT TGGGGAAGAC CATCCAATCT ATCACATTCT TACAAGAAGT ATATAATGTG 
GGAATACGGG GACCTTTTTT AGTAATTGCT CCACTTTCCA CCATCACGAA CTGGGAGAGA 
GAGTTTGGCA GTTGGACTCA AATGAATACT ATTGTGTATC ATGGCAGCCT TGCTAGTCGC 
CAAATGATCC AGCAATATGA GATGTACTGT AAGGACTCCA AGGGTCGTCT TATTCCTGGT 
GCGTACAAGT TTGATGCTCT TATTACAACT TTTGAAATGG TGCTGTCTGA TTGCCCAGAG 
CTGCGTGAAA TTGAGTGGCG CTGTGTCATA ATTGATGAGG CTCATCGGCT CAAAAACAGA 
AACTGCAAAC TACTGGACAG CCTAAAGCAC ATGGACTTGG AGCACAAAGT CCTTTTAACA 
GGCACTCCAT TACAGAACAC CGTGGAAGAG TTGTTCAGTC TTTTGCATTT CCTGGAGCCA 
ACACAATTTT CCTCTGAAGC AGAGTTTCTT AAGGACTTTG GAGACCTGAA GACAGAGGAA 
CAGGTGCAGA AATTACAGGC CATCTTAAAA CCTATGATGT TGAGGCGATT AAAGGAAGAT 
GTTGAGAAAA ACCTGGCCCC GAAACAAGAA ACTATAATTG AGGTTGAGCT TACTAACATC 
CAGAAGAAGT ATTACCGAGC AATACTGGAG AAGAATTTTT CTTTTCTGAC CAAAGGAGCT 
AGTCAATCCA ACACACCCAA CTTGCTCAAT ACCATGATGG AACTACGCAA ATGCTGCAAC 
CACCCATACC TCATCACTGG AGCAGAAGAA AAGATCATAT CTGAGTTCCG AGAAGCAACT 
CCAGTAGTTC CCCCTGACTT TCACGTCCAA GCAATGGTAC GCTCCTCTGG CAAACTGGTG 
CTAATAGATA AGCTGCTCCC TAAACTAAGG GCTGGAGGCC ACAAAGTTCT CATCTTTTCT 
CAGATGGTAC GGTGCCTGGA CATCCTGGAA GACTATCTCA TTCAGAGAAG GTACCTTTAT 
GAGCGTATTG ATGGACGTGT CCGTGGCAAC ATGCGTCAGG CAGCCATTGA TAGATTCAGC 
CGCCCAGATT CTGACCGATT TGTGTTTCTA CTCTGTACAC GAGCTGGTGG GCTTGGAATT 
AACTTGACAG CTGCTGATAC TTGTATTATA TTTGACTCGG ACTGGAACCC TCAAAATGAC 
CTTCAGGCTC AAGCTCGATG TCATCGTATT GGGCAAAGCA AAGCCGTTAA AATTTACAGA 
CTCATCACTC GCAACTCCTA TGAGAGAGAG ATGTTTGATA AGGCCAGTCT CAAACTAGGA 
CTAGACAAAG CTGTATTGCA GTCTATGAGT GGTCGGGATA ATCACCTTAG TGGGCCTATT 
CAGCAATTCA CAAAGAAAGA AATTGAAGAC TTGCTAAGGA AAGGAGCTTA TGCTGCAATC 
ATGGATGAAG ATGACGAAGG CTCTAAATTC TGTGAAGAAG ATATTGACCA AATTTTACTG 
AGGCGTACCA CGACTATTAC CATAGAGTCA GAGGGAAAGG GCTCAACCTT CTCCAAGGCA 
AGTTTTGTTG CTTCTGAGAA CAGGACTGAT ATATCCCTAG ATGATCCTAA TTTCTGGCAA 
AAGTGGGCCA AGAAAGCAGA CTTAGACTTG GATCTTCTTA GCAGCAAGAA TACACTGGTT 
ATTGATACCC CACGTATCAG GAAGCAAACA CGGCACTTTA CTAACAAAGA TGATGACATG 
GTTGAGTTCT CAGATTTGGA GAGTGATGAT GATGATCGCC CCAAAGCTCG GCGTCAAGAC 
CGCAAGCATG GATATGGACG TACAGATTGC TTCCGTGTGG AGAAGCATTT GCTTGTTTAT 
GGATGGGGTC GCTGGCGAGA TATCCTTACT CATGGTCGTT TCAAGCGTGG TATGAATGAG 
CGTGACGTGG AGAAAATATG TAGAGCCATA CTGGTTTATT GTCTTTTGCA CTACCGGGGA 
GATGAAAACA TAAAGAGCTT TATCTGGGAT CTAATTACAC CAGCTGAGAA TGGCAAAACT 
AAGCAGCTAC AGAACCACTC TGGATTGTCT ATTCCTGTCC CCCGTGGTCG TAAGGGGAAA 
AAAGTGAAAT CCCAAAGCAG CTTTGACATA CATAAAGCCG ATTGGATACA TAAGTACAAC 
CCAGATACCT TGTTCCAAGA TGAAGGGTAC AAGAAACATC TCAAGCATCA ATGTAACAAG 
GTGCTGCTTC GCGTTCGTAT GTTATATTTT CTTCGTCAGG AAGTTATTGG AAACCAGGCA 
ATGAAAGTTT TAAGTGGTGT AGAGGCCAGG CAAATTGACA TCTGGTTCCC ACAGGTGGAC 
CAGGTTGAAG TTCCCTCACG CTGGTGGGAC AGAGAAGCTG ATAAATCTCT TCTTATCGGG 
GTCTTTAAAC ATGGTTATGA GAAGTATAAC ACCATGCGAG CTGACCCTGC ACTCTGTTTT 
TTGGAGAAGG TGGGGGGACC AGATGAACAG GCTATTGCAG CTGAGCACCA TGCTCAAGAC 
TTTTCTGAAC TCCCTGATGG TGGGGACTTT GATCGAGACA TAGAAGATCC AGAATACAAG 
CCACTTCATG CACAGAAAGA TCCTGAGGAT GAGATCGACT CTTTAATGAT GGATGAAGAA 
ATCTCTGTTG TTGACGGTGA AGAAGCAACA GCAATGCCCA GTGGTATGTT ATGGCCACCT 
GGCTCTGCTC TCACAGCTCG TCTACGGCGT CTAATTACTG CCTACCAACG CAGCTACAAA 
AGAGAACAAC TAAAATTGGA GGCTGAAGAG CGTGGGGACA AACGTAGAAA ACGATGTGAG 
GCAGCCTTTA AACTGAAAGA AATTGCCCGT AGGGAGAAAC AGCAAAGGTG GACCCGAAGG 
GAGGCATGTG ACTTTTTTCG TGTGCTGTCC AGCTTTGGTG TCGAGTATGA CCCCGATACT 
CAACTGTATG ACTGGCAGAG GTTCCGTGGT CTGGCAAGAC TGGACAAGAA GACAGATGAG 
TCACTGCTCA AATACTTCCA TGGATTTGTG GCAATGTGCC GCCAGGTTTG CCGCTTACCC 
CCTGCTGCCG GAGACGAGCC ACCAGATCCA TCTCTCTTCA TTGAGCCAAT TTCAGAAGAG 
CGAGCATCTC GCGCCCTGTT TCGCTTGGAT CTTCTTCGTA GGGTGCGTGA GCAAGTACTT 
TGTCATCCTT TGCTTATTCC TCGTTTGTCA CTGTGCCGGC CAGACCCAGA ACTCCCAGAA 
TGGTGGGAGA GTGGACGTCA TGACAATGAG CTTCTTCAGG GAGCAGCACG TTATGGTCTG 
AGCCGTACAG ACCTCACTAT ACTGCAGGAC CATGCATTTT CTTTTTTGCG GTGTCAGATC 
AGTTATTTCC AGTCAAGGGG AATATCTGGC CATTCTCGCC CTTCTACTCC AACTACTGCC 
CCTTCAGTGC CTACTGAACG TCAGCCTTCT CATCTAGCCT CCCTTTCCTC ATCTGTCAGC 
TGTTCTCCTG CTCCACGCAG ATCCTCTTCC TGCTCATCAT CATCTCACTC ATCAAACTCT 
GAGGATTCTT CAGATGAAAG CAATGGTGTA AAAGTAAAAC CTAATGGTCT TTCGCACGCT 
CGACTGTATG ATGAGGAGAG CCGGCTTTCT CTTACTGCCT CCCCTGCAGA TCTCACTACA 
GAGGACAGTA TTCAGACAGC GCTGGAGACC CCGCACAGTA CAGACTGGCC AAAGGACCGT 
GTCCTTATCT GCAGAATTGA GCAAGTATGC AGTGCCGTTC TTACTGGCAA ATGGTCCTCA 
CCACGACGAC TGTCAGATCC ACCAAGTGAC AGCCCTGATA GCTTGCCTCC AACCCCAGAG 
CAGCAGAGTC CAGCCCACTT CACACAGATA AGACCTGCAC CGGACCCCAA AGAGTTTACC 
ATTCAAATAA AAAGTGAAGA AGGCCTGAAG ATTAAATTTC AGAAGCACAA ATTTATGGGT 
AATGGAGCTC TTGATTCTGC ACCTCATACC CAGAAGAAAA AGAGCAAGAA AAAGATGGAT 
CTTGACATGG ACACACGAAT TCCCGTTGTA AACCAGAAGG ATGGGACCTT GTTGTTAGGG 
GAAGAAGCCC CTTTACGCTG CCAGCTGCCC GAGTGGTTAC ACAGAAATCC TGACTTCATG 
GTTGACCCAC GCTTTATTGC GTTTATGGAA GACCGCAGGA AAAGAAAAAA ACATCGAAGC 
AAAAAGTCCA AATCCTCCTC CCAGATTGGA AACGTACAGA ATCCCCACTC CCACAATGGA 
AAGAAGTCTT CCCACACAGA AAACTCTTTC GAGACGCAAC ACCTTCCTAC TGCAGCCAAA 
AAAAGGAAGA AACGCCCTCC TTCAAAAATA ATAATGCCAT CTGCACTACA TGGAGCTGGT 
GATGTTACAC ATTCCTTCTC AGACCTTCAA CCTGACCATT CTGGTCACCC CATGCGGTCA 
GTGTATGGAC CATCAGATGG AGGGCTGTCC CATCAAGCAG GACTCAGCGG GTTGGGATTT 
ACCCGCTTTC ATTCATCGCA GACATCTTCA GTGGGCAGAA TCCATGGCAC TACCTTTGAT 
GAGTTACAAA GTTTTGAGCC CCCACAACAA CATTTTTCTC TGCTTGATGA TCCTATGATG 
CCAGCAAATT CCTCCTCCAG CGGTGATGAA CTGGACAGAC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012812652.1
CDS	301..7305
Translation	MADPIMDLFDDPNLFGLDSLTGDSFGRDGPDTIDDALGLGNVLGPLEPITDRVGLPGADVGNNEVKQQTESQVLPDNPALMSATEIMQPIQLPTNQETLNQGNPFMGASNAGAPKIMILKAPAGMTVGACPVTQIQTLTPHQAANGGKVAFAKVLTGTPLRPGVSIVSGNTVLAKMPSTGVAGQVGAVRPVRQLLLQPVRASAAPGSSESNTGIKPAITLTSAPQQGDPKRITLVLQQPSQVGATAQQGQRHVVLGGLPGKIVLQGNQLAALTQAKTPQGQPAKVVTIQLQVQQQPGAAGQTPQKFQIVQQAPGGVAIAPSGQHTHMLNQQGVQRLSVPLKVVLQPQAGSSQGTAQGLSVVKVLNASEVANLTASQTVVKTSTGGGESRKLDSQKKQEKANRIVAEAIARARARGEQNIPRVLNEDELPSVNPEDDDGSRRKRKKKKGETSDRSKDEKPKKVKGSGSLRSRSKGKPSTITPIVGKKRKRNPSSDNSDAEIMASQASPAEDEDSVQKRRSNRQVKRKKYTEDLDIKITDDEDDEEVDVTGPVKTEPVLLPEPMPLPDSEAVPSMQFFVENPSEEDAAIVDKILSMRLAKKELPTGEYVEVEEYFVKYKNYSYLHCEWATIEQLERDKRIHQKLKRFKTKMTQMQHFLQEDEESFNPDYVEVDRILDESHSTDKDNGEPVVYYLVKWCSLPYEDSTWELKEDVDDGKIEEFKRIEARQPNLKRVARPAATSWKKLELSREYQNGNQLREYQLEGVNWLLFNWYNRQNCILADEMGLGKTIQSITFLQEVYNVGIRGPFLVIAPLSTITNWEREFGSWTQMNTIVYHGSLASRQMIQQYEMYCKDSKGRLIPGAYKFDALITTFEMVLSDCPELREIEWRCVIIDEAHRLKNRNCKLLDSLKHMDLEHKVLLTGTPLQNTVEELFSLLHFLEPTQFSSEAEFLKDFGDLKTEEQVQKLQAILKPMMLRRLKEDVEKNLAPKQETIIEVELTNIQKKYYRAILEKNFSFLTKGASQSNTPNLLNTMMELRKCCNHPYLITGAEEKIISEFREATPVVPPDFHVQAMVRSSGKLVLIDKLLPKLRAGGHKVLIFSQMVRCLDILEDYLIQRRYLYERIDGRVRGNMRQAAIDRFSRPDSDRFVFLLCTRAGGLGINLTAADTCIIFDSDWNPQNDLQAQARCHRIGQSKAVKIYRLITRNSYEREMFDKASLKLGLDKAVLQSMSGRDNHLSGPIQQFTKKEIEDLLRKGAYAAIMDEDDEGSKFCEEDIDQILLRRTTTITIESEGKGSTFSKASFVASENRTDISLDDPNFWQKWAKKADLDLDLLSSKNTLVIDTPRIRKQTRHFTNKDDDMVEFSDLESDDDDRPKARRQDRKHGYGRTDCFRVEKHLLVYGWGRWRDILTHGRFKRGMNERDVEKICRAILVYCLLHYRGDENIKSFIWDLITPAENGKTKQLQNHSGLSIPVPRGRKGKKVKSQSSFDIHKADWIHKYNPDTLFQDEGYKKHLKHQCNKVLLRVRMLYFLRQEVIGNQAMKVLSGVEARQIDIWFPQVDQVEVPSRWWDREADKSLLIGVFKHGYEKYNTMRADPALCFLEKVGGPDEQAIAAEHHAQDFSELPDGGDFDRDIEDPEYKPLHAQKDPEDEIDSLMMDEEISVVDGEEATAMPSGMLWPPGSALTARLRRLITAYQRSYKREQLKLEAEERGDKRRKRCEAAFKLKEIARREKQQRWTRREACDFFRVLSSFGVEYDPDTQLYDWQRFRGLARLDKKTDESLLKYFHGFVAMCRQVCRLPPAAGDEPPDPSLFIEPISEERASRALFRLDLLRRVREQVLCHPLLIPRLSLCRPDPELPEWWESGRHDNELLQGAARYGLSRTDLTILQDHAFSFLRCQISYFQSRGISGHSRPSTPTTAPSVPTERQPSHLASLSSSVSCSPAPRRSSSCSSSSHSSNSEDSSDESNGVKVKPNGLSHARLYDEESRLSLTASPADLTTEDSIQTALETPHSTDWPKDRVLICRIEQVCSAVLTGKWSSPRRLSDPPSDSPDSLPPTPEQQSPAHFTQIRPAPDPKEFTIQIKSEEGLKIKFQKHKFMGNGALDSAPHTQKKKSKKKMDLDMDTRIPVVNQKDGTLLLGEEAPLRCQLPEWLHRNPDFMVDPRFIAFMEDRRKRKKHRSKKSKSSSQIGNVQNPHSHNGKKSSHTENSFETQHLPTAAKKRKKRPPSKIIMPSALHGAGDVTHSFSDLQPDHSGHPMRSVYGPSDGGLSHQAGLSGLGFTRFHSSQTSSVGRIHGTTFDELQSFEPPQQHFSLLDDPMMPANSSSSGDELDRP

Target ORF information:

RefSeq Version	XM_012957198.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis chromodomain helicase DNA binding protein 8 (chd8), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957198.2

ORF Insert Sequence:

ATGGCTGACC CTATCATGGA CCTCTTTGAT GATCCAAACC TATTTGGCCT TGACTCTCTC 
ACTGGAGATT CCTTTGGACG GGATGGGCCA GACACTATAG ATGATGCTCT AGGCCTGGGT 
AATGTCTTGG GACCTTTGGA GCCGATAACA GACAGAGTTG GCCTGCCTGG TGCTGATGTT 
GGCAATAATG AAGTTAAACA ACAAACAGAG TCACAGGTTC TCCCGGACAA TCCAGCTCTT 
ATGTCAGCTA CTGAGATCAT GCAGCCCATT CAACTTCCCA CAAACCAGGA AACACTGAAC 
CAAGGAAATC CCTTTATGGG TGCCTCAAAT GCTGGAGCCC CAAAAATTAT GATTCTGAAA 
GCTCCTGCTG GAATGACAGT TGGTGCATGT CCTGTAACTC AGATACAAAC TCTGACACCT 
CACCAAGCTG CTAATGGTGG CAAGGTGGCA TTTGCTAAGG TGCTTACAGG AACCCCATTG 
CGTCCTGGGG TCTCAATTGT ATCTGGAAAC ACTGTCCTTG CCAAAATGCC AAGCACTGGA 
GTTGCAGGGC AGGTAGGAGC AGTGAGGCCT GTCCGTCAGC TACTTCTTCA ACCAGTGCGT 
GCCTCCGCAG CCCCAGGAAG CTCCGAGAGT AATACAGGGA TCAAGCCTGC AATCACACTA 
ACGTCAGCAC CTCAACAGGG GGACCCTAAA AGAATTACTC TCGTCCTACA GCAGCCTTCC 
CAAGTTGGTG CAACTGCACA ACAAGGGCAG CGTCATGTTG TGCTGGGTGG GCTTCCAGGA 
AAGATTGTCC TTCAGGGAAA CCAACTGGCA GCTCTCACAC AAGCTAAAAC CCCTCAAGGT 
CAGCCAGCTA AAGTAGTGAC TATCCAGCTG CAAGTCCAGC AGCAGCCTGG GGCTGCAGGT 
CAGACTCCTC AAAAGTTTCA GATTGTACAA CAAGCCCCAG GAGGTGTGGC AATTGCTCCC 
AGTGGGCAAC ATACACACAT GCTTAATCAA CAAGGAGTTC AGAGGCTTTC TGTTCCACTC 
AAAGTTGTTC TGCAACCACA GGCTGGTTCC TCTCAGGGTA CTGCGCAGGG CCTGTCAGTT 
GTGAAAGTTC TAAATGCAAG TGAAGTTGCC AATTTGACTG CAAGTCAGAC TGTGGTTAAA 
ACATCCACTG GAGGTGGTGA ATCTCGTAAG CTGGACAGCC AGAAGAAGCA AGAGAAAGCT 
AACCGTATTG TAGCTGAAGC TATTGCCAGG GCCCGAGCCA GAGGTGAACA GAATATTCCT 
CGGGTCCTGA ATGAGGATGA GCTTCCCAGT GTGAATCCTG AAGATGATGA TGGAAGCAGG 
CGGAAAAGGA AGAAGAAGAA AGGAGAGACT AGTGATAGGT CCAAGGATGA GAAACCTAAG 
AAGGTCAAAG GTAGTGGAAG CTTAAGGTCT AGGAGCAAAG GCAAACCAAG TACTATCACT 
CCTATTGTGG GTAAGAAAAG GAAGAGAAAC CCATCTTCCG ATAATTCTGA TGCTGAGATC 
ATGGCATCAC AGGCTTCTCC TGCAGAGGAT GAAGACAGTG TCCAGAAGAG ACGTTCAAAT 
CGCCAGGTGA AGCGCAAGAA GTATACAGAG GATTTGGATA TTAAAATAAC AGATGATGAA 
GATGATGAGG AGGTAGATGT GACGGGACCT GTGAAAACAG AACCTGTGCT GCTTCCTGAG 
CCCATGCCTT TACCTGACAG TGAGGCAGTC CCTTCAATGC AGTTCTTCGT GGAGAACCCA 
AGTGAAGAAG ATGCTGCTAT TGTTGACAAA ATACTCTCTA TGCGCCTAGC TAAAAAAGAG 
CTGCCCACTG GAGAGTATGT TGAAGTTGAA GAGTACTTTG TCAAATATAA AAATTATTCC 
TACCTACACT GTGAATGGGC CACAATTGAG CAACTGGAAA GGGACAAGCG TATTCATCAG 
AAACTGAAAA GATTTAAGAC AAAGATGACC CAGATGCAGC ATTTCTTGCA AGAAGATGAA 
GAATCTTTCA ATCCAGACTA TGTAGAGGTA GATCGAATCC TAGACGAATC TCACAGCACT 
GACAAGGACA ATGGTGAACC GGTGGTGTAT TACCTAGTAA AATGGTGCTC TTTACCTTAT 
GAAGATAGCA CATGGGAGCT GAAAGAAGAT GTTGATGATG GAAAGATAGA GGAATTCAAA 
CGAATAGAGG CGAGGCAGCC AAATCTCAAA CGCGTGGCCC GCCCAGCTGC TACATCATGG 
AAGAAGCTGG AATTGTCTCG GGAGTATCAG AATGGCAACC AGTTGAGAGA ATACCAGCTG 
GAAGGTGTTA ATTGGCTTCT CTTTAACTGG TACAATAGGC AAAACTGCAT TTTGGCAGAT 
GAAATGGGTT TGGGGAAGAC CATCCAATCT ATCACATTCT TACAAGAAGT ATATAATGTG 
GGAATACGGG GACCTTTTTT AGTAATTGCT CCACTTTCCA CCATCACGAA CTGGGAGAGA 
GAGTTTGGCA GTTGGACTCA AATGAATACT ATTGTGTATC ATGGCAGCCT TGCTAGTCGC 
CAAATGATCC AGCAATATGA GATGTACTGT AAGGACTCCA AGGGTCGTCT TATTCCTGGT 
GCGTACAAGT TTGATGCTCT TATTACAACT TTTGAAATGG TGCTGTCTGA TTGCCCAGAG 
CTGCGTGAAA TTGAGTGGCG CTGTGTCATA ATTGATGAGG CTCATCGGCT CAAAAACAGA 
AACTGCAAAC TACTGGACAG CCTAAAGCAC ATGGACTTGG AGCACAAAGT CCTTTTAACA 
GGCACTCCAT TACAGAACAC CGTGGAAGAG TTGTTCAGTC TTTTGCATTT CCTGGAGCCA 
ACACAATTTT CCTCTGAAGC AGAGTTTCTT AAGGACTTTG GAGACCTGAA GACAGAGGAA 
CAGGTGCAGA AATTACAGGC CATCTTAAAA CCTATGATGT TGAGGCGATT AAAGGAAGAT 
GTTGAGAAAA ACCTGGCCCC GAAACAAGAA ACTATAATTG AGGTTGAGCT TACTAACATC 
CAGAAGAAGT ATTACCGAGC AATACTGGAG AAGAATTTTT CTTTTCTGAC CAAAGGAGCT 
AGTCAATCCA ACACACCCAA CTTGCTCAAT ACCATGATGG AACTACGCAA ATGCTGCAAC 
CACCCATACC TCATCACTGG AGCAGAAGAA AAGATCATAT CTGAGTTCCG AGAAGCAACT 
CCAGTAGTTC CCCCTGACTT TCACGTCCAA GCAATGGTAC GCTCCTCTGG CAAACTGGTG 
CTAATAGATA AGCTGCTCCC TAAACTAAGG GCTGGAGGCC ACAAAGTTCT CATCTTTTCT 
CAGATGGTAC GGTGCCTGGA CATCCTGGAA GACTATCTCA TTCAGAGAAG GTACCTTTAT 
GAGCGTATTG ATGGACGTGT CCGTGGCAAC ATGCGTCAGG CAGCCATTGA TAGATTCAGC 
CGCCCAGATT CTGACCGATT TGTGTTTCTA CTCTGTACAC GAGCTGGTGG GCTTGGAATT 
AACTTGACAG CTGCTGATAC TTGTATTATA TTTGACTCGG ACTGGAACCC TCAAAATGAC 
CTTCAGGCTC AAGCTCGATG TCATCGTATT GGGCAAAGCA AAGCCGTTAA AATTTACAGA 
CTCATCACTC GCAACTCCTA TGAGAGAGAG ATGTTTGATA AGGCCAGTCT CAAACTAGGA 
CTAGACAAAG CTGTATTGCA GTCTATGAGT GGTCGGGATA ATCACCTTAG TGGGCCTATT 
CAGCAATTCA CAAAGAAAGA AATTGAAGAC TTGCTAAGGA AAGGAGCTTA TGCTGCAATC 
ATGGATGAAG ATGACGAAGG CTCTAAATTC TGTGAAGAAG ATATTGACCA AATTTTACTG 
AGGCGTACCA CGACTATTAC CATAGAGTCA GAGGGAAAGG GCTCAACCTT CTCCAAGGCA 
AGTTTTGTTG CTTCTGAGAA CAGGACTGAT ATATCCCTAG ATGATCCTAA TTTCTGGCAA 
AAGTGGGCCA AGAAAGCAGA CTTAGACTTG GATCTTCTTA GCAGCAAGAA TACACTGGTT 
ATTGATACCC CACGTATCAG GAAGCAAACA CGGCACTTTA CTAACAAAGA TGATGACATG 
GTTGAGTTCT CAGATTTGGA GAGTGATGAT GATGATCGCC CCAAAGCTCG GCGTCAAGAC 
CGCAAGCATG GATATGGACG TACAGATTGC TTCCGTGTGG AGAAGCATTT GCTTGTTTAT 
GGATGGGGTC GCTGGCGAGA TATCCTTACT CATGGTCGTT TCAAGCGTGG TATGAATGAG 
CGTGACGTGG AGAAAATATG TAGAGCCATA CTGGTTTATT GTCTTTTGCA CTACCGGGGA 
GATGAAAACA TAAAGAGCTT TATCTGGGAT CTAATTACAC CAGCTGAGAA TGGCAAAACT 
AAGCAGCTAC AGAACCACTC TGGATTGTCT ATTCCTGTCC CCCGTGGTCG TAAGGGGAAA 
AAAGTGAAAT CCCAAAGCAG CTTTGACATA CATAAAGCCG ATTGGATACA TAAGTACAAC 
CCAGATACCT TGTTCCAAGA TGAAGGGTAC AAGAAACATC TCAAGCATCA ATGTAACAAG 
GTGCTGCTTC GCGTTCGTAT GTTATATTTT CTTCGTCAGG AAGTTATTGG AAACCAGGCA 
ATGAAAGTTT TAAGTGGTGT AGAGGCCAGG CAAATTGACA TCTGGTTCCC ACAGGTGGAC 
CAGGTTGAAG TTCCCTCACG CTGGTGGGAC AGAGAAGCTG ATAAATCTCT TCTTATCGGG 
GTCTTTAAAC ATGGTTATGA GAAGTATAAC ACCATGCGAG CTGACCCTGC ACTCTGTTTT 
TTGGAGAAGG TGGGGGGACC AGATGAACAG GCTATTGCAG CTGAGCACCA TGCTCAAGAC 
TTTTCTGAAC TCCCTGATGG TGGGGACTTT GATCGAGACA TAGAAGATCC AGAATACAAG 
CCACTTCATG CACAGAAAGA TCCTGAGGAT GAGATCGACT CTTTAATGAT GGATGAAGAA 
ATCTCTGTTG TTGACGGTGA AGAAGCAACA GCAATGCCCA GTGGTATGTT ATGGCCACCT 
GGCTCTGCTC TCACAGCTCG TCTACGGCGT CTAATTACTG CCTACCAACG CAGCTACAAA 
AGAGAACAAC TAAAATTGGA GGCTGAAGAG CGTGGGGACA AACGTAGAAA ACGATGTGAG 
GCAGCCTTTA AACTGAAAGA AATTGCCCGT AGGGAGAAAC AGCAAAGGTG GACCCGAAGG 
GAGGCATGTG ACTTTTTTCG TGTGCTGTCC AGCTTTGGTG TCGAGTATGA CCCCGATACT 
CAACTGTATG ACTGGCAGAG GTTCCGTGGT CTGGCAAGAC TGGACAAGAA GACAGATGAG 
TCACTGCTCA AATACTTCCA TGGATTTGTG GCAATGTGCC GCCAGGTTTG CCGCTTACCC 
CCTGCTGCCG GAGACGAGCC ACCAGATCCA TCTCTCTTCA TTGAGCCAAT TTCAGAAGAG 
CGAGCATCTC GCGCCCTGTT TCGCTTGGAT CTTCTTCGTA GGGTGCGTGA GCAAGTACTT 
TGTCATCCTT TGCTTATTCC TCGTTTGTCA CTGTGCCGGC CAGACCCAGA ACTCCCAGAA 
TGGTGGGAGA GTGGACGTCA TGACAATGAG CTTCTTCAGG GAGCAGCACG TTATGGTCTG 
AGCCGTACAG ACCTCACTAT ACTGCAGGAC CATGCATTTT CTTTTTTGCG GTGTCAGATC 
AGTTATTTCC AGTCAAGGGG AATATCTGGC CATTCTCGCC CTTCTACTCC AACTACTGCC 
CCTTCAGTGC CTACTGAACG TCAGCCTTCT CATCTAGCCT CCCTTTCCTC ATCTGTCAGC 
TGTTCTCCTG CTCCACGCAG ATCCTCTTCC TGCTCATCAT CATCTCACTC ATCAAACTCT 
GAGGATTCTT CAGATGAAAG CAATGGTGTA AAAGTAAAAC CTAATGGTCT TTCGCACGCT 
CGACTGTATG ATGAGGAGAG CCGGCTTTCT CTTACTGCCT CCCCTGCAGA TCTCACTACA 
GAGGACAGTA TTCAGACAGC GCTGGAGACC CCGCACAGTA CAGACTGGCC AAAGGACCGT 
GTCCTTATCT GCAGAATTGA GCAAGTATGC AGTGCCGTTC TTACTGGCAA ATGGTCCTCA 
CCACGACGAC TGTCAGATCC ACCAAGTGAC AGCCCTGATA GCTTGCCTCC AACCCCAGAG 
CAGCAGAGTC CAGCCCACTT CACACAGATA AGACCTGCAC CGGACCCCAA AGAGTTTACC 
ATTCAAATAA AAAGTGAAGA AGGCCTGAAG ATTAAATTTC AGAAGCACAA ATTTATGGGT 
AATGGAGCTC TTGATTCTGC ACCTCATACC CAGAAGAAAA AGAGCAAGAA AAAGATGGAT 
CTTGACATGG ACACACGAAT TCCCGTTGTA AACCAGAAGG ATGGGACCTT GTTGTTAGGG 
GAAGAAGCCC CTTTACGCTG CCAGCTGCCC GAGTGGTTAC ACAGAAATCC TGACTTCATG 
GTTGACCCAC GCTTTATTGC GTTTATGGAA GACCGCAGGA AAAGAAAAAA ACATCGAAGC 
AAAAAGTCCA AATCCTCCTC CCAGATTGGA AACGTACAGA ATCCCCACTC CCACAATGGA 
AAGAAGTCTT CCCACACAGA AAACTCTTTC GAGACGCAAC ACCTTCCTAC TGCAGCCAAA 
AAAAGGAAGA AACGCCCTCC TTCAAAAATA ATAATGCCAT CTGCACTACA TGGAGCTGGT 
GATGTTACAC ATTCCTTCTC AGACCTTCAA CCTGACCATT CTGGTCACCC CATGCGGTCA 
GTGTATGGAC CATCAGATGG AGGGCTGTCC CATCAAGCAG GACTCAGCGG GTTGGGATTT 
ACCCGCTTTC ATTCATCGCA GACATCTTCA GTGGGCAGAA TCCATGGCAC TACCTTTGAT 
GAGTTACAAA GTTTTGAGCC CCCACAACAA CATTTTTCTC TGCTTGATGA TCCTATGATG 
CCAGCAAATT CCTCCTCCAG CGGTGATGAA CTGGACAGAC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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